DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0023/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0023/defocus_estimation/series0023_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0023/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -52.8 40500.0 35000.0 34500.0 1 -50.6 36000.0 33500.0 41500.0 2 -48.4 33000.0 33500.0 44500.0 3 -46.2 32000.0 39500.0 40000.0 4 -44.0 32000.0 33000.0 40500.0 5 -41.8 32000.0 48000.0 32000.0 6 -39.6 32500.0 32000.0 32500.0 7 -37.4 32000.0 32000.0 47500.0 8 -35.2 33500.0 37500.0 47000.0 9 -33.0 33000.0 40500.0 45000.0 10 -30.8 32000.0 32000.0 33500.0 11 -28.6 32000.0 48000.0 42500.0 12 -26.4 32000.0 47000.0 48000.0 13 -24.2 45500.0 48000.0 48000.0 14 -22.0 39500.0 44000.0 46000.0 15 -19.8 47500.0 47000.0 44500.0 16 -17.6 33500.0 32000.0 33000.0 17 -15.4 32000.0 32000.0 33500.0 18 -13.2 32000.0 32000.0 32000.0 19 -11.0 32000.0 38500.0 41000.0 20 -8.8 32000.0 32500.0 32000.0 21 -6.6 32500.0 32500.0 32000.0 22 -4.4 43500.0 48000.0 36000.0 23 -2.2 32000.0 32000.0 32000.0 24 -0.0 38000.0 32000.0 32000.0 25 0.0 32500.0 33000.0 32000.0 26 2.2 33500.0 32500.0 32000.0 27 4.4 46500.0 32000.0 34000.0 28 6.6 32000.0 32000.0 32000.0 29 8.8 32500.0 33500.0 37000.0 30 11.0 32000.0 32000.0 32500.0 31 13.2 39000.0 45000.0 34500.0 32 15.4 43000.0 32500.0 32000.0 33 17.6 48000.0 48000.0 45500.0 34 19.8 36000.0 36500.0 32000.0 35 22.0 36500.0 47500.0 32000.0 36 24.2 46000.0 46000.0 32500.0 37 26.4 47500.0 48000.0 32500.0 38 28.6 48000.0 47500.0 34500.0 39 30.8 47500.0 34000.0 39000.0 40 33.0 46000.0 40500.0 32500.0 41 35.2 46500.0 41000.0 34000.0 42 37.4 46000.0 48000.0 33500.0 43 39.6 47500.0 47000.0 33000.0 44 41.8 43000.0 44500.0 33000.0 45 44.0 43000.0 41000.0 33000.0 46 46.2 45000.0 41000.0 48000.0 47 48.4 39500.0 34000.0 44500.0 48 50.6 41500.0 48000.0 32000.0 49 52.8 33000.0 34000.0 36000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)