DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0028/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0028/defocus_estimation/series0028_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0028/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 41.8 39500.0 44000.0 43500.0 1 39.6 45000.0 40000.0 33000.0 2 37.4 45000.0 45000.0 33500.0 3 35.2 46000.0 35000.0 32500.0 4 33.0 36500.0 42500.0 34000.0 5 30.8 46000.0 34500.0 37500.0 6 28.6 48000.0 46000.0 37500.0 7 26.4 40000.0 44000.0 41500.0 8 24.2 45500.0 45000.0 36000.0 9 22.0 40000.0 43500.0 32000.0 10 19.8 32000.0 44500.0 34500.0 11 17.6 45000.0 32000.0 37500.0 12 15.4 44000.0 45500.0 43000.0 13 13.2 43500.0 47500.0 32000.0 14 11.0 45000.0 44500.0 32500.0 15 8.8 32000.0 47000.0 33000.0 16 6.6 41500.0 45000.0 34000.0 17 4.4 41000.0 37500.0 33500.0 18 2.2 44500.0 46500.0 37500.0 19 0.0 46000.0 46500.0 41000.0 20 -0.0 32000.0 44500.0 43000.0 21 -2.2 44000.0 42500.0 38000.0 22 -4.4 45000.0 43500.0 36500.0 23 -6.6 45000.0 44500.0 47000.0 24 -8.8 44000.0 43500.0 44500.0 25 -11.0 44000.0 46000.0 44000.0 26 -13.2 43500.0 44500.0 32500.0 27 -15.4 40000.0 44500.0 43500.0 28 -17.6 44500.0 45500.0 46000.0 29 -19.8 38000.0 45500.0 46000.0 30 -22.0 48000.0 44500.0 44500.0 31 -24.2 42500.0 41500.0 35000.0 32 -26.4 45500.0 36000.0 43500.0 33 -28.6 40000.0 39000.0 35500.0 34 -30.8 40000.0 32000.0 48000.0 35 -33.0 43000.0 41500.0 32000.0 36 -35.2 33500.0 40000.0 48000.0 37 -37.4 42000.0 44000.0 47500.0 38 -39.6 41500.0 40500.0 33500.0 39 -41.8 42000.0 34000.0 43000.0 40 -44.0 37000.0 34000.0 33500.0 41 -46.2 38000.0 39500.0 34000.0 42 -48.4 41000.0 40500.0 45500.0 43 -50.6 39000.0 32000.0 45000.0 44 -52.8 32500.0 44000.0 34500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)