DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0030/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0030/defocus_estimation/series0030_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0030/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 46.2 34500.0 47000.0 45500.0 1 44.0 34500.0 35000.0 41000.0 2 41.8 44500.0 42500.0 38500.0 3 39.6 38500.0 33500.0 41500.0 4 37.4 38500.0 48000.0 40500.0 5 35.2 32000.0 41500.0 33000.0 6 33.0 32500.0 37500.0 43000.0 7 30.8 34000.0 32500.0 37000.0 8 28.6 33000.0 32500.0 32000.0 9 26.4 32000.0 35500.0 32000.0 10 24.2 36000.0 34500.0 34500.0 11 22.0 33000.0 34500.0 32000.0 12 19.8 35000.0 32000.0 32000.0 13 17.6 36500.0 40500.0 34500.0 14 15.4 36500.0 33000.0 36500.0 15 13.2 40000.0 34000.0 33000.0 16 11.0 36500.0 36500.0 39000.0 17 8.8 36500.0 34500.0 34000.0 18 6.6 33500.0 37000.0 34500.0 19 4.4 35000.0 36500.0 36500.0 20 2.2 35500.0 34500.0 34000.0 21 0.0 33500.0 34000.0 36000.0 22 -0.0 33500.0 33000.0 34000.0 23 -2.2 32500.0 34000.0 34000.0 24 -4.4 34500.0 32000.0 33000.0 25 -6.6 33500.0 33500.0 33000.0 26 -8.8 32500.0 32000.0 32000.0 27 -11.0 34000.0 32000.0 33000.0 28 -13.2 32500.0 32000.0 34000.0 29 -15.4 32500.0 33000.0 33000.0 30 -17.6 33500.0 32000.0 33500.0 31 -19.8 32000.0 32500.0 34000.0 32 -22.0 32000.0 35000.0 33000.0 33 -24.2 32000.0 32000.0 34000.0 34 -26.4 33000.0 32000.0 32000.0 35 -28.6 33000.0 34000.0 32000.0 36 -30.8 36500.0 33000.0 44000.0 37 -33.0 32000.0 33000.0 32000.0 38 -35.2 45500.0 34500.0 32000.0 39 -37.4 40000.0 47000.0 44000.0 40 -39.6 47000.0 46000.0 34500.0 41 -41.8 37500.0 35000.0 32000.0 42 -44.0 38500.0 42000.0 43500.0 43 -46.2 41500.0 35000.0 36000.0 44 -48.4 38500.0 40000.0 34500.0 45 -50.6 40000.0 46500.0 37500.0 46 -52.8 40000.0 32000.0 42500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 94.792u 1.291s 1:36.21 99.8% 0+0k 0+0io 0pf+0w