DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0039/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0039/defocus_estimation/series0039_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0039/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 30.8 33000.0 32000.0 32000.0 1 28.6 34500.0 34000.0 33500.0 2 26.4 35000.0 33000.0 32500.0 3 24.2 32000.0 33500.0 32500.0 4 22.0 34000.0 34500.0 32000.0 5 19.8 34500.0 32500.0 32500.0 6 17.6 33500.0 35000.0 33500.0 7 15.4 33000.0 34000.0 34500.0 8 13.2 39000.0 34000.0 33000.0 9 11.0 36000.0 35000.0 35500.0 10 8.8 38000.0 35000.0 34000.0 11 6.6 35500.0 34500.0 34500.0 12 4.4 35500.0 35000.0 35000.0 13 2.2 35000.0 34500.0 35000.0 14 0.0 35500.0 35000.0 34500.0 15 -0.0 35000.0 34500.0 34000.0 16 -2.2 34500.0 34500.0 33500.0 17 -4.4 34500.0 34500.0 34500.0 18 -6.6 34500.0 34500.0 32500.0 19 -8.8 34500.0 33000.0 32500.0 20 -11.0 33000.0 34000.0 34500.0 21 -13.2 33000.0 34500.0 32500.0 22 -15.4 33500.0 34000.0 34500.0 23 -17.6 34000.0 33500.0 34500.0 24 -19.8 33000.0 33500.0 34500.0 25 -22.0 33000.0 34000.0 32000.0 26 -24.2 33000.0 33000.0 32000.0 27 -26.4 32500.0 32500.0 34500.0 28 -28.6 33000.0 33500.0 32500.0 29 -30.8 32000.0 33000.0 33500.0 30 -33.0 32500.0 33500.0 34500.0 31 -35.2 32000.0 32500.0 33000.0 32 -37.4 32000.0 33000.0 37000.0 33 -39.6 32000.0 32000.0 34000.0 34 -41.8 32000.0 43500.0 32000.0 35 -44.0 32500.0 32000.0 32500.0 36 -46.2 45500.0 32000.0 40000.0 37 -48.4 32000.0 33000.0 34000.0 38 -50.6 37500.0 32000.0 34500.0 39 -52.8 46000.0 36000.0 35500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 83.444u 0.972s 1:24.51 99.8% 0+0k 0+0io 0pf+0w