DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0041/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0041/defocus_estimation/series0041_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0041/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 33.0 36000.0 33500.0 33000.0 1 30.8 35500.0 34500.0 32500.0 2 28.6 36000.0 34000.0 32500.0 3 26.4 36000.0 36500.0 33500.0 4 24.2 36000.0 34000.0 32500.0 5 22.0 35500.0 34500.0 33000.0 6 19.8 37500.0 34500.0 34500.0 7 17.6 37000.0 36000.0 33500.0 8 15.4 36500.0 35500.0 33000.0 9 13.2 37500.0 36500.0 33500.0 10 11.0 37000.0 36000.0 38000.0 11 8.8 37500.0 37000.0 36000.0 12 6.6 37000.0 37000.0 35000.0 13 4.4 36500.0 38000.0 37500.0 14 2.2 37000.0 37500.0 39000.0 15 0.0 37000.0 38500.0 36500.0 16 -0.0 33500.0 33500.0 32500.0 17 -2.2 34000.0 33500.0 34500.0 18 -4.4 33000.0 33500.0 32000.0 19 -6.6 33000.0 33500.0 33000.0 20 -8.8 33000.0 33500.0 32500.0 21 -11.0 33000.0 33000.0 33000.0 22 -13.2 32500.0 34000.0 32500.0 23 -15.4 32000.0 33000.0 35000.0 24 -17.6 32000.0 33000.0 33000.0 25 -19.8 32000.0 33500.0 33000.0 26 -22.0 32000.0 32500.0 33000.0 27 -24.2 32000.0 34000.0 33000.0 28 -26.4 32000.0 32500.0 33000.0 29 -28.6 32000.0 33000.0 32500.0 30 -30.8 32000.0 32000.0 32000.0 31 -33.0 32000.0 32000.0 33000.0 32 -35.2 32000.0 32000.0 33000.0 33 -37.4 32500.0 32000.0 33000.0 34 -39.6 34500.0 32000.0 33000.0 35 -41.8 44500.0 34500.0 47500.0 36 -44.0 43500.0 36000.0 36500.0 37 -46.2 44500.0 40500.0 33500.0 38 -48.4 43000.0 46500.0 33000.0 39 -50.6 45500.0 45000.0 33000.0 40 -52.8 41500.0 47500.0 48000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 94.461u 1.002s 1:35.58 99.8% 0+0k 0+0io 0pf+0w