DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0049/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0049/defocus_estimation/series0049_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/18nov10g/protomo_alignments/tiltseries0049/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 32000.0, 48000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 44.0 33000.0 39000.0 38500.0 1 41.8 33500.0 36500.0 36500.0 2 39.6 36500.0 40000.0 37500.0 3 37.4 43500.0 38000.0 33500.0 4 35.2 33000.0 41500.0 36000.0 5 33.0 33000.0 32500.0 32000.0 6 30.8 32000.0 33500.0 38500.0 7 28.6 35500.0 41500.0 40500.0 8 26.4 44500.0 36000.0 39500.0 9 24.2 46500.0 33500.0 38500.0 10 22.0 40000.0 38500.0 36500.0 11 19.8 46000.0 33000.0 38500.0 12 17.6 37500.0 41000.0 36500.0 13 15.4 32000.0 33000.0 36500.0 14 13.2 40000.0 41000.0 36000.0 15 11.0 32000.0 33500.0 38000.0 16 8.8 43500.0 41500.0 38500.0 17 6.6 32000.0 38000.0 38500.0 18 4.4 36500.0 41000.0 33500.0 19 2.2 36500.0 32000.0 32000.0 20 0.0 38000.0 32000.0 32000.0 21 -0.0 32000.0 32000.0 32000.0 22 -2.2 38000.0 32000.0 32000.0 23 -4.4 33000.0 33000.0 32000.0 24 -6.6 44500.0 32000.0 32000.0 25 -8.8 32000.0 32000.0 32000.0 26 -11.0 32500.0 43500.0 34500.0 27 -13.2 36500.0 35000.0 32500.0 28 -15.4 39500.0 38500.0 32000.0 29 -17.6 32000.0 32000.0 32000.0 30 -19.8 42500.0 32000.0 32000.0 31 -22.0 46500.0 32000.0 32000.0 32 -24.2 39500.0 40000.0 40500.0 33 -26.4 37500.0 38500.0 32000.0 34 -28.6 38000.0 39500.0 40000.0 35 -30.8 39000.0 42000.0 32000.0 36 -33.0 32500.0 41500.0 32000.0 37 -35.2 34500.0 38000.0 32000.0 38 -37.4 40500.0 45500.0 32500.0 39 -39.6 41000.0 37000.0 32000.0 40 -41.8 36000.0 38500.0 32000.0 41 -44.0 33500.0 40000.0 34000.0 42 -46.2 33000.0 38000.0 32000.0 43 -48.4 48000.0 35500.0 32000.0 44 -50.6 36000.0 40000.0 32000.0 45 -52.8 48000.0 33500.0 36500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 105.683u 1.114s 1:48.28 98.6% 0+0k 0+0io 0pf+0w