DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19mar29h/protomo_alignments/tiltseries0001/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19mar29h/protomo_alignments/tiltseries0001/defocus_estimation/series0001_tilts_negative.txt Output diagnostic file name: /gpfs/appion/jbrasch/19mar29h/protomo_alignments/tiltseries0001/defocus_estimation//diagnostic_negative.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 28344.3, 42516.51, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 58.0 28000.0 31000.0 35500.0 1 56.0 35000.0 30500.0 28000.0 2 54.0 35000.0 31000.0 29000.0 3 52.0 33500.0 31000.0 28000.0 4 50.0 33000.0 30500.0 29500.0 5 48.0 32000.0 29000.0 28500.0 6 46.0 30000.0 29500.0 30500.0 7 44.0 32500.0 29000.0 28000.0 8 42.0 30500.0 28500.0 28500.0 9 40.0 30000.0 28000.0 29000.0 10 38.0 30000.0 28000.0 28000.0 11 36.0 29000.0 28000.0 31000.0 12 34.0 30000.0 28000.0 30500.0 13 32.0 30000.0 28000.0 28000.0 14 30.0 28500.0 28000.0 35000.0 15 28.0 29000.0 28000.0 28000.0 16 26.0 37500.0 28500.0 32000.0 17 24.0 28500.0 28000.0 33500.0 18 22.0 29000.0 28000.0 29000.0 19 20.0 30000.0 28500.0 28000.0 20 18.0 29000.0 28000.0 36000.0 21 16.0 29500.0 28500.0 28000.0 22 14.0 29000.0 28500.0 28000.0 23 12.0 29000.0 28500.0 28000.0 24 10.0 29000.0 28500.0 28000.0 25 10.0 28500.0 28000.0 28000.0 26 8.0 28000.0 28000.0 28000.0 27 6.0 28000.0 28000.0 28000.0 28 4.0 28000.0 28000.0 28000.0 29 2.0 28000.0 28000.0 28000.0 30 0.0 28000.0 28000.0 28000.0 31 -2.0 28000.0 28000.0 28000.0 32 -4.0 28000.0 28000.0 28000.0 33 -6.0 28000.0 28000.0 28000.0 34 -8.0 28000.0 28000.0 28000.0 35 -10.0 28000.0 28000.0 28500.0 36 -12.0 28000.0 28500.0 30500.0 37 -14.0 28000.0 28000.0 28000.0 38 -16.0 35000.0 28000.0 28000.0 39 -18.0 28000.0 28500.0 29000.0 40 -20.0 34000.0 34000.0 28500.0 41 -22.0 36000.0 30000.0 28500.0 42 -24.0 31500.0 28000.0 32500.0 43 -26.0 38500.0 30000.0 28000.0 44 -28.0 34500.0 36000.0 29500.0 45 -30.0 34500.0 28000.0 28500.0 46 -32.0 34500.0 35500.0 28000.0 47 -34.0 33000.0 34500.0 28000.0 48 -36.0 33500.0 36000.0 28000.0 49 -38.0 34500.0 28000.0 28000.0 50 -40.0 33000.0 35000.0 28000.0 51 -42.0 34000.0 34500.0 33000.0 52 -44.0 28000.0 35000.0 28000.0 53 -46.0 32500.0 34500.0 35500.0 54 -48.0 34000.0 31500.0 34500.0 55 -50.0 33000.0 28000.0 28000.0 56 -52.0 32000.0 32500.0 29000.0 57 -54.0 28000.0 30500.0 35000.0 58 -56.0 30000.0 30000.0 28500.0 59 -58.0 31500.0 35500.0 28000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles are according to the convention used in TOMOCTF: i.e., images with positive tilt angle have increasing underfocus magnitude with the X axis (left to right) 123.631u 1.389s 2:05.21 99.8% 0+0k 0+0io 0pf+0w