DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/jbrasch/19mar29h/protomo_alignments/tiltseries0012/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/jbrasch/19mar29h/protomo_alignments/tiltseries0012/defocus_estimation/series0012_tilts.txt Output diagnostic file name: /gpfs/appion/jbrasch/19mar29h/protomo_alignments/tiltseries0012/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 27702.9, 41554.29, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -58.0 32000.0 30500.0 29000.0 1 -56.0 33500.0 32000.0 29500.0 2 -54.0 32000.0 33000.0 29500.0 3 -52.0 32000.0 30500.0 27500.0 4 -50.0 32000.0 29000.0 27500.0 5 -48.0 31500.0 29500.0 28500.0 6 -46.0 29000.0 28000.0 27500.0 7 -44.0 29000.0 28000.0 39000.0 8 -42.0 28000.0 27500.0 27500.0 9 -40.0 29500.0 27500.0 34500.0 10 -38.0 28000.0 27500.0 38500.0 11 -36.0 36500.0 27500.0 29500.0 12 -34.0 28000.0 27500.0 34500.0 13 -32.0 29500.0 27500.0 27500.0 14 -30.0 27500.0 27500.0 32000.0 15 -28.0 27500.0 27500.0 36000.0 16 -26.0 36500.0 27500.0 30000.0 17 -24.0 28000.0 27500.0 30500.0 18 -22.0 28000.0 35500.0 34500.0 19 -20.0 27500.0 27500.0 38500.0 20 -18.0 38000.0 27500.0 32500.0 21 -16.0 28500.0 27500.0 36000.0 22 -14.0 27500.0 27500.0 27500.0 23 -12.0 32000.0 27500.0 35000.0 24 -10.0 30500.0 27500.0 39000.0 25 -10.0 28000.0 27500.0 28000.0 26 -8.0 28000.0 29500.0 28000.0 27 -6.0 27500.0 28000.0 27500.0 28 -4.0 27500.0 29500.0 28500.0 29 -2.0 30500.0 27500.0 27500.0 30 0.0 27500.0 27500.0 27500.0 31 2.0 27500.0 29500.0 27500.0 32 4.0 27500.0 28000.0 28500.0 33 6.0 27500.0 28000.0 28000.0 34 8.0 27500.0 28500.0 28000.0 35 10.0 29500.0 28500.0 36500.0 36 12.0 28000.0 27500.0 27500.0 37 14.0 28500.0 27500.0 28000.0 38 16.0 29000.0 28500.0 28000.0 39 18.0 34500.0 27500.0 27500.0 40 20.0 27500.0 27500.0 27500.0 41 22.0 27500.0 34500.0 29000.0 42 24.0 33500.0 37000.0 28000.0 43 26.0 38000.0 28500.0 36500.0 44 28.0 27500.0 27500.0 27500.0 45 30.0 28500.0 28500.0 28000.0 46 32.0 30000.0 27500.0 28000.0 47 34.0 27500.0 28000.0 27500.0 48 36.0 35500.0 28500.0 27500.0 49 38.0 38000.0 30000.0 38000.0 50 40.0 36000.0 36000.0 28000.0 51 42.0 32000.0 29500.0 36000.0 52 44.0 27500.0 35000.0 27500.0 53 46.0 27500.0 34500.0 27500.0 54 48.0 27500.0 27500.0 28500.0 55 50.0 34000.0 33500.0 28000.0 56 52.0 32000.0 33500.0 29000.0 57 54.0 31000.0 29000.0 34000.0 58 56.0 30000.0 34500.0 29500.0 59 58.0 41500.0 32500.0 29500.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right) 130.560u 1.417s 2:13.27 99.0% 0+0k 0+0io 0pf+0w