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        <keyDates>
            <depositionDate>2020-04-17</depositionDate>
            <releaseDate>2020-09-02</releaseDate>
            <updateDate>2020-09-02</updateDate>
        </keyDates>
        <title>CryoEM reconstruction of ESCRT-III filament composed of IST1 NTD R16E K27E double mutant</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-2231-2577</authorORCID>
            <firstName>Adam</firstName>
            <lastName>Frost</lastName>
            <organization type="academic">Univeristy of California, San Fancisco</organization>
            <street>Genetech Hall S472F (office), 600 16th Street</street>
            <townOrCity>San Francisco</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94158</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-2231-2577</authorORCID>
            <firstName>Adam</firstName>
            <lastName>Frost</lastName>
            <organization type="academic">Univeristy of California, San Fancisco</organization>
            <street>Genetech Hall S472F (office), 600 16th Street</street>
            <townOrCity>San Francisco</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94158</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-6142-8807">Nguyen HC</author>
            <author authorORCID="0000-0002-4603-0120">Talledge N</author>
            <author authorORCID="0000-0001-6836-4394">McCullough J</author>
            <author>Sharma A</author>
            <author authorORCID="0000-0002-6149-6447">Moss FR</author>
            <author>Iwasa JH</author>
            <author>Vershinin MD</author>
            <author>Sundquist WI</author>
            <author authorORCID="0000-0003-2231-2577">Frost A</author>
        </authorsList>
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        <entryDOI>10.6019/EMPIAR-10398</entryDOI>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-20591</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
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                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-6142-8807" order="1">Nguyen HC</author>
                    <author authorORCID="0000-0002-4603-0120" order="2">Talledge N</author>
                    <author authorORCID="0000-0001-6836-4394" order="3">McCullough J</author>
                    <author order="4">Sharma A</author>
                    <author authorORCID="0000-0002-6149-6447" order="5">Moss FR</author>
                    <author order="6">Iwasa JH</author>
                    <author order="7">Vershinin MD</author>
                    <author order="8">Sundquist WI</author>
                    <author authorORCID="0000-0003-2231-2577" order="9">Frost A</author>
                    <title>Membrane constriction and thinning by sequential ESCRT-III polymerization</title>
                    <journal>Nature structural &amp; molecular biology</journal>
                    <journalAbbreviation>Nat. Struct. Mol. Biol.</journalAbbreviation>
                    <country></country>
                    <issue>4</issue>
                    <volume>27</volume>
                    <firstPage>392</firstPage>
                    <lastPage>399</lastPage>
                    <year>2020</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41594-020-0404-x</externalReferences>
                    <externalReferences type="pubmed">32251413</externalReferences>
                    <details>Processed particle stacks and associated metadata for RELION helical reconstruction.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>CryoEM data of ESCRT-III filament composed of IST1 NTD R16E K27E double mutant</name>
        <directory>/data</directory>
        <category>picked particles - single frame - processed</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>4556</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>320</imageWidth>
            <pixelWidth>1.23</pixelWidth>
            <imageHeight>320</imageHeight>
            <pixelHeight>1.23</pixelHeight>
        </dimensions>
        <details>particles used to reconstruct an IST1 double mutant alone using helical reconstruction in RELION</details>
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        <micrographsFilePattern>data/IST1dm_job058.mrcs</micrographsFilePattern>
        <pickedParticlesFilePattern>data/IST1dm_job058.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
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