*** Entries to program alignframes *** InputFile = 556_001.tif OutputImageFile = W:/Modis_02July2018/images/556_001_ali.mrc AlignAndSumBinning = 4 1 ScalingOfSum = 12.225000 GainReferenceFile = CountRef_58-4_001.dm4 RotationAndFlip = -1 CameraDefectFile = defects_455_001.txt PairwiseFrames = 40 ShiftLimit = 20 GroupSize = 1 FilterRadius2 = 0.060000 VaryFilter = 0.060000 RefineAlignment = 5 StopIterationsAtShift = 0.100000 RefineRadius2 = 0.060000 MinForSplineSmoothing = 0 UseGPU = 0 AntialiasFilter = 4 FilterSigma2 = 0.008574 *** End of entries *** Assuming binning of 1.0 instead of a small subarea; use the -imagebinned option to set a binning if this is incorrect. File 1 (556_001.tif): 40 frames Weighted residual mean = 0.646, SD = 0.285, mean max = 1.05, max max = 1.05 Max unweighted resid mean = 2.95, max = 2.95 Dist = 16.43, smoothed = 14.22 FRC crossings 0.5: 0.0352 0.25: 0.0413 0.125: 0.0447 is 0.0634 at 0.25/pix SUCCESSFULLY COMPLETED