*** Entries to program alignframes *** InputFile = 58-28_001.tif OutputImageFile = W:/Modis_02July2018/images/58-28_001_ali.mrc AlignAndSumBinning = 4 1 ScalingOfSum = 12.225000 GainReferenceFile = CountRef_58-4_001.dm4 RotationAndFlip = -1 CameraDefectFile = defects_58-4_001.txt PairwiseFrames = 40 ShiftLimit = 20 GroupSize = 1 FilterRadius2 = 0.060000 VaryFilter = 0.060000 RefineAlignment = 5 StopIterationsAtShift = 0.100000 RefineRadius2 = 0.060000 MinForSplineSmoothing = 0 UseGPU = 0 AntialiasFilter = 4 FilterSigma2 = 0.008574 *** End of entries *** Assuming binning of 1.0 instead of a small subarea; use the -imagebinned option to set a binning if this is incorrect. File 1 (58-28_001.tif): 40 frames Weighted residual mean = 0.135, SD = 0.058, mean max = 0.21, max max = 0.21 Max unweighted resid mean = 0.47, max = 0.47 Dist = 11.78, smoothed = 10.73 FRC crossings 0.5: 0.0635 0.25: 0.0686 0.125: 0.0712 is 0.1892 at 0.25/pix SUCCESSFULLY COMPLETED