*** Entries to program alignframes *** InputFile = 58-88_003.tif OutputImageFile = W:/Modis_02July2018/images/58-88_003_ali.mrc AlignAndSumBinning = 4 1 ScalingOfSum = 12.225000 GainReferenceFile = CountRef_58-4_001.dm4 RotationAndFlip = -1 CameraDefectFile = defects_58-4_001.txt PairwiseFrames = 40 ShiftLimit = 20 GroupSize = 1 FilterRadius2 = 0.060000 VaryFilter = 0.060000 RefineAlignment = 5 StopIterationsAtShift = 0.100000 RefineRadius2 = 0.060000 MinForSplineSmoothing = 0 UseGPU = 0 AntialiasFilter = 4 FilterSigma2 = 0.008574 *** End of entries *** Assuming binning of 1.0 instead of a small subarea; use the -imagebinned option to set a binning if this is incorrect. File 1 (58-88_003.tif): 40 frames Weighted residual mean = 0.736, SD = 0.347, mean max = 1.25, max max = 1.25 Max unweighted resid mean = 4.44, max = 4.44 Dist = 11.65, smoothed = 4.88 FRC crossings 0.5: 0.0065 0.25: 0.0122 0.125: 0.0659 is 0.0680 at 0.25/pix SUCCESSFULLY COMPLETED