*** Entries to program alignframes *** InputFile = 58-89_003.tif OutputImageFile = W:/Modis_02July2018/images/58-89_003_ali.mrc AlignAndSumBinning = 4 1 ScalingOfSum = 12.225000 GainReferenceFile = CountRef_58-4_001.dm4 RotationAndFlip = -1 CameraDefectFile = defects_58-4_001.txt PairwiseFrames = 40 ShiftLimit = 20 GroupSize = 1 FilterRadius2 = 0.060000 VaryFilter = 0.060000 RefineAlignment = 5 StopIterationsAtShift = 0.100000 RefineRadius2 = 0.060000 MinForSplineSmoothing = 0 UseGPU = 0 AntialiasFilter = 4 FilterSigma2 = 0.008574 *** End of entries *** Assuming binning of 1.0 instead of a small subarea; use the -imagebinned option to set a binning if this is incorrect. File 1 (58-89_003.tif): 40 frames Weighted residual mean = 0.664, SD = 0.303, mean max = 1.09, max max = 1.09 Max unweighted resid mean = 3.58, max = 3.58 Dist = 10.04, smoothed = 4.21 FRC crossings 0.5: 0.0065 0.25: 0.0572 0.125: 0.0633 is 0.0710 at 0.25/pix SUCCESSFULLY COMPLETED