*** Entries to program alignframes *** InputFile = 639_001.tif OutputImageFile = W:/Modis_02July2018/images/639_001_ali.mrc AlignAndSumBinning = 4 1 ScalingOfSum = 12.225000 GainReferenceFile = CountRef_58-4_001.dm4 RotationAndFlip = -1 CameraDefectFile = defects_455_001.txt PairwiseFrames = 40 ShiftLimit = 20 GroupSize = 1 FilterRadius2 = 0.060000 VaryFilter = 0.060000 RefineAlignment = 5 StopIterationsAtShift = 0.100000 RefineRadius2 = 0.060000 MinForSplineSmoothing = 0 UseGPU = 0 AntialiasFilter = 4 FilterSigma2 = 0.008574 *** End of entries *** Assuming binning of 1.0 instead of a small subarea; use the -imagebinned option to set a binning if this is incorrect. File 1 (639_001.tif): 40 frames Weighted residual mean = 0.728, SD = 0.319, mean max = 1.18, max max = 1.18 Max unweighted resid mean = 3.51, max = 3.51 Dist = 11.97, smoothed = 7.90 FRC crossings 0.5: 0.0095 0.25: 0.0527 0.125: 0.0579 is 0.0610 at 0.25/pix SUCCESSFULLY COMPLETED