*** Entries to program alignframes *** InputFile = 70-77_002.tif OutputImageFile = W:/Modis_02July2018/images/70-77_002_ali.mrc AlignAndSumBinning = 4 1 ScalingOfSum = 12.225000 GainReferenceFile = CountRef_58-4_001.dm4 RotationAndFlip = -1 CameraDefectFile = defects_64-104_001.txt PairwiseFrames = 40 ShiftLimit = 20 GroupSize = 1 FilterRadius2 = 0.060000 VaryFilter = 0.060000 RefineAlignment = 5 StopIterationsAtShift = 0.100000 RefineRadius2 = 0.060000 MinForSplineSmoothing = 0 UseGPU = 0 AntialiasFilter = 4 FilterSigma2 = 0.008574 *** End of entries *** Assuming binning of 1.0 instead of a small subarea; use the -imagebinned option to set a binning if this is incorrect. File 1 (70-77_002.tif): 40 frames Weighted residual mean = 0.651, SD = 0.293, mean max = 1.07, max max = 1.07 Max unweighted resid mean = 2.96, max = 2.96 Dist = 10.52, smoothed = 4.39 SUCCESSFULLY COMPLETED