*** Entries to program alignframes *** InputFile = 76-30_002.tif OutputImageFile = W:/Modis_02July2018/images/76-30_002_ali.mrc AlignAndSumBinning = 4 1 ScalingOfSum = 12.225000 GainReferenceFile = CountRef_58-4_001.dm4 RotationAndFlip = -1 CameraDefectFile = defects_64-104_001.txt PairwiseFrames = 40 ShiftLimit = 20 GroupSize = 1 FilterRadius2 = 0.060000 VaryFilter = 0.060000 RefineAlignment = 5 StopIterationsAtShift = 0.100000 RefineRadius2 = 0.060000 MinForSplineSmoothing = 0 UseGPU = 0 AntialiasFilter = 4 FilterSigma2 = 0.008574 *** End of entries *** Assuming binning of 1.0 instead of a small subarea; use the -imagebinned option to set a binning if this is incorrect. File 1 (76-30_002.tif): 40 frames Weighted residual mean = 0.619, SD = 0.284, mean max = 1.02, max max = 1.02 Max unweighted resid mean = 3.02, max = 3.02 Dist = 11.10, smoothed = 7.09 FRC crossings 0.5: 0.0167 0.25: 0.0455 0.125: 0.0490 is 0.0739 at 0.25/pix SUCCESSFULLY COMPLETED