CGRP receptor bound to CGRP dataset

Sample: RAMP1-CLR-CGRP (CGRPR-CGRP)
Grid: UltrAuFoil 300 mesh R1.2/1.3
Cemera: Gatan K3 post a BioQuantum energy filter
Camera mode: CDS, counting (hardware binning by 2)
TEM mode: EFTEM NanoProbe
Mag: x105k
C2 aperture: 50 um
Spot: 5
Beam diameter: 1.6 um
EF slit: 25 eV
Data collection software: SerialEM
Acquisition method: beam-image shift with beam-tilt compensation
Acquisition pattern: 3 x 3 holes, 1 image in the center of each hole
Physical Pixel: 0.83 A/pix
Exposure rate:  7.2 e/pix/s (CDS)
Exposure time:  5.011 s
Total exposure: 52.4 e/A2 
Frames: 71
Defocus: 0.7 - 1.6 um
Movies: 5598
Data format: non-gain-normalized LZW compressed TIFFs
Gain reference: "RAMP1-CGRP_gain.mrc" prepared from the data
Final particles: 388k
Particles file: "CGRPR-CGRP_data.star"
Optics groups: per-acquisition position
Final resolution: 3.5 A

Rado
March 19, 2021