GLP-1R-GLP-1 dataset

Sample: GLP-1R in complex woith GLP-1 and Gs
Grid: UltrAuFoil 300 mesh R1.2/1.3
Cemera: Gatan K3 post a BioQuantum energy filter
Camera mode: counting (hardware binning by 2)
TEM mode: EFTEM NanoProbe
Mag: x105k
C2 aperture: 50 um
Spot: 4
Beam diameter: 1.45 um
EF slit: 25 eV
Data collection software: SerialEM
Acquisition method: beam-image shift WITHOUT beam-tilt compensation
Acquisition pattern: 3 x 3 holes, 1 image in the center of each hole
Physical Pixel: 0.83 A/pix
Exposure rate:  15.0 e/pix/s
Exposure time:  2.996 s
Total exposure: 65.3 e/A2 
Frames: 75
Defocus: 0.6 - 1.4 um
Movies: 5739
Data format: non-gain-normalized LZW compressed TIFFs
Gain reference: "GLP-1_gain.mrc" prepared from the data
Final particles: 636k
Particles file: "GLP-1_data.star"
Optics groups: per-image-shift position
Final resolution: 2.11 A

Rado
March 23, 2021