To test the overall performance of the Krios G4 at The University of Tokyo, five datasets were collected from the same apoferritin grid in the beginning of July 2020.
This was the second dataset in the series.

The dataset was semi-intentionally collected with ~1.2 mrad beam tilt. This served as a good test for the 3D reconstruction software's ability to measure and correct strong aberrations.

The illumination conditions (beam diameter & spot size) were changed for set2 without re-doing the coma-free alignment, which would have required unloading the apoF grid and loading a carbon film grid.

Sample: mouse heavy chain apoferritin
Grid: UltrAuFoil 300 mesh R1.2/1.3
Cemera: Gatan K3 post a BioQuantum energy filter
Camera mode: super resolution, CDS
TEM mode: EFTEM NanoProbe
Mag: x215k
C2 aperture: 50 um
Spot: 6
Beam diameter: 0.65 um
EF slit: 15 eV
Data collection software: SerialEM
Acquisition method: beam-image shift with beam-tilt compensation
Acquisition pattern: 3 x 3 holes, 4 images around the periphery of each hole
Physical Pixel: 0.3994 A/pix
SuperRes pixel: 0.1997 A/pix
Exposure rate:  3.54 e/pix/s (CDS)
Exposure time:  3.04 s
Total exposure: 67.1 e/A2 
Frames: 76
Defocus: 0.2 - 0.8 um
Movies: 2484
Data format: non-gain-normalized LZW compressed TIFFs
Gain reference: "apoF_set2_gain.mrc" prepared from the data
Final particles: 136k
Particles file: "apoF_set2_data.star"
Optics groups: per-image-shift position
Resolution (with Ewald correction): 1.35 A
Rosenthal-Henderson B-factor: 38.3 A^2


Rado
March 25, 2021