17-Nov-21 19:44:30 Trimming A by 65 65, reference by 65 65 Trimming A by 65 65, reference by 65 65 Trimming A by 60 65, reference by 65 65 Trimming A by 65 65, reference by 65 65 Rough eucentricity: changing Z by 0.35 to -6.20, finished. 24693.703: (Initial) alignment to map 32 (54) 24693.703: Target X, Y: -200.550 332.714 24693.718: Map matches low dose View; staying in low dose but with exp 0.405 intensity 0.19459 bin 4 Trimming A by 60 24 60 20, reference by 47 22 70 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.01 Aligning to target with image shift equivalent to stage shift: -0.11 -0.02 24701.187: Map matches low dose Preview; staying in low dose but with exp 0.202 intensity 0.12072 bin 4 24713.328: Realigned to map item itself with image shift of -3.7 -20.8 pixels Disparity in stage position after aligning to map item itself: -0.11 -0.02 (align shift 0.01 -0.03) Finished aligning to item in 2 rounds Opened new file W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004.mrc STARTING TILT SERIES WITH AUTOMATIC TERMINATION AFTER ERRORS. 24721.218: Skipping reverse tilt: dir -1 start 0.00 revang 3.00 revtilt 2.99 maxtilt 69.90 maxerr 0.10 Measured defocus = -9.29 microns changed by 9.19 to target Measured defocus = 0.19 microns changed by -0.29 to target 24755.671: SetIntensity called with factor 0.9962 24755.671: Changing (ideal) Record exposure by 0.9962 to 1.5918 24755.671: Frame time for constraint is 0.1598 24755.687: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 24755.687: Skipping reverse tilt: dir -1 start -9.99 revang -6.99 revtilt 2.99 maxtilt 69.90 maxerr 0.10 Need regular tracking - Need autofocus Tracking image shifted 0.003, 0.005 um; position 0.125, 0.327 Measured defocus = -0.01 microns changed by -0.09 to target Tracking image shifted 0.000, -0.002 um; position 0.125, 0.329 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0000_-10.0.tif Frame alignment results: distance raw = 8.2 smoothed = 7.7 Weighted resid mean = 0.04 max max = 0.07 Mean unweighted max = 0.19 Saved Z = 0 at 0.00 min Tilt = -9.99 Mean = 41 Exp = 1.592 X/Y/Z = 0.125 0.329 3.914 24775.359: Saved state 0 at -10.0: def 3.814 IS -0.263 0.224 int 0.12072 exp 1.592 24775.359: SetIntensity called with factor 1.0002 24775.359: Changing (ideal) Record exposure by 1.0002 to 1.5920 24775.359: Frame time for constraint is 0.1598 24775.375: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Need regular tracking - Need autofocus Tracking image shifted -0.008, 0.016 um; position 0.132, 0.313 Measured defocus = -0.21 microns changed by 0.11 to target Tracking image shifted -0.000, -0.002 um; position 0.132, 0.315 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0001_-12.0.tif Frame alignment results: distance raw = 5.0 smoothed = 4.1 Weighted resid mean = 0.04 max max = 0.06 Mean unweighted max = 0.15 Record image shifted 0.001, -0.007 um Tracking reference shifted by 0.0,5.5 pixels, 0.001,-0.007 um Saved Z = 1 at 0.48 min Tilt = -11.99 Mean = 42 Exp = 1.592 X/Y/Z = 0.131 0.322 4.027 24804.156: Saved state 1 at -12.0: def 3.927 IS -0.264 0.214 int 0.12072 exp 1.592 24804.156: SetIntensity called with factor 1.0005 24804.156: Changing (ideal) Record exposure by 1.0005 to 1.5927 24804.156: Frame time for constraint is 0.1598 24804.156: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Predicted X = 0.137 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.315 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 4.140 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.025 um; position 0.136, 0.289 Measured defocus = -0.16 microns changed by 0.06 to target Tracking image shifted -0.000, -0.002 um; position 0.137, 0.292 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0002_-14.0.tif Frame alignment results: distance raw = 3.5 smoothed = 2.8 Weighted resid mean = 0.04 max max = 0.06 Mean unweighted max = 0.11 Record image shifted 0.001, -0.007 um Tracking reference shifted by 0.1,5.2 pixels, 0.001,-0.007 um Saved Z = 2 at 0.96 min Tilt = -13.99 Mean = 42 Exp = 1.593 X/Y/Z = 0.135 0.299 4.200 24833.062: Saved state 2 at -14.0: def 4.100 IS -0.257 0.191 int 0.12072 exp 1.593 24833.140: Restored state 0 at -10.0 for tiltInd 3 def 3.814 IS -0.263 0.224 24833.140: SetIntensity called with factor 1.0002 24833.140: Changing (ideal) Record exposure by 1.0002 to 1.5920 24833.140: Frame time for constraint is 0.1598 24833.156: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 24836.765: recentlyReversed: dir 1 ang -7.991521 revtilt -5.000387 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.002 -0.016 um, 0.1 1.2 % Error in Z Prediction 0.060 Need regular tracking - Need autofocus Tracking image shifted 0.054, -0.100 um; position 0.071, 0.429 Measured defocus = 0.05 microns changed by -0.15 to target Tracking image shifted 0.000, -0.003 um; position 0.071, 0.431 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0003_-8.0.tif Frame alignment results: distance raw = 4.0 smoothed = 4.0 Weighted resid mean = 0.05 max max = 0.07 Mean unweighted max = 0.18 Record image shifted 0.001, 0.006 um Tracking reference shifted by -1.2,-4.4 pixels, 0.001,0.006 um Saved Z = 3 at 1.48 min Tilt = -7.99 Mean = 41 Exp = 1.592 X/Y/Z = 0.070 0.425 3.764 24864.125: Saved state 3 at -8.0: def 3.664 IS -0.262 0.338 int 0.12072 exp 1.592 24864.125: SetIntensity called with factor 1.0005 24864.140: Changing (ideal) Record exposure by 1.0005 to 1.5927 24864.140: Frame time for constraint is 0.1598 24864.140: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 24867.625: recentlyReversed: dir 1 ang -5.997765 revtilt -3.000132 back 3.000000 3mte 0.300000 Need regular tracking - Need autofocus Tracking image shifted 0.003, 0.012 um; position 0.067, 0.413 Measured defocus = -0.01 microns changed by -0.09 to target Tracking image shifted -0.000, -0.001 um; position 0.068, 0.414 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0004_-6.0.tif Frame alignment results: distance raw = 4.7 smoothed = 4.2 Weighted resid mean = 0.04 max max = 0.06 Mean unweighted max = 0.15 Record image shifted 0.001, 0.005 um Tracking reference shifted by -1.0,-3.4 pixels, 0.001,0.005 um Saved Z = 4 at 1.99 min Tilt = -6.00 Mean = 41 Exp = 1.593 X/Y/Z = 0.067 0.409 3.672 24894.953: Saved state 4 at -6.0: def 3.572 IS -0.252 0.325 int 0.12072 exp 1.593 24895.031: Restored state 2 at -14.0 for tiltInd 5 def 4.100 IS -0.257 0.191 24895.031: SetIntensity called with factor 1.0008 24895.031: Changing (ideal) Record exposure by 1.0008 to 1.5940 24895.031: Frame time for constraint is 0.1598 24895.046: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Predicted X = 0.141 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.286 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Z = 4.333 StdErr = 0.045 nFit = 3 nDrop = 0 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0005_-16.0.tif Frame alignment results: distance raw = 5.5 smoothed = 4.5 Weighted resid mean = 0.04 max max = 0.06 Mean unweighted max = 0.21 Record image shifted 0.051, -0.034 um Saved Z = 5 at 2.29 min Tilt = -15.99 Mean = 42 Exp = 1.594 X/Y/Z = 0.090 0.321 4.333 24913.000: Saved state 5 at -16.0: def 4.233 IS -0.229 0.235 int 0.12072 exp 1.594 24913.000: SetIntensity called with factor 1.0011 24913.000: Changing (ideal) Record exposure by 1.0011 to 1.5957 24913.015: Frame time for constraint is 0.1598 24913.015: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.051 0.034 um, 3.7 2.5 % Predicted X = 0.078 StdErr = 0.037 nFit = 3 nDrop = 0 Predicted Y = 0.312 StdErr = 0.034 nFit = 3 nDrop = 0 Predicted Z = 4.476 StdErr = 0.059 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.012, -0.049 um; position 0.090, 0.361 Measured defocus = 0.01 microns changed by -0.11 to target Tracking image shifted 0.000, -0.003 um; position 0.089, 0.365 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0006_-18.0.tif Frame alignment results: distance raw = 3.0 smoothed = 2.0 Weighted resid mean = 0.04 max max = 0.07 Mean unweighted max = 0.20 Record image shifted 0.002, -0.014 um Tracking reference shifted by 0.4,10.2 pixels, 0.002,-0.014 um Saved Z = 6 at 2.77 min Tilt = -17.99 Mean = 42 Exp = 1.596 X/Y/Z = 0.087 0.378 4.371 24941.750: Saved state 6 at -18.0: def 4.271 IS -0.254 0.287 int 0.12072 exp 1.596 24941.828: Restored state 4 at -6.0 for tiltInd 7 def 3.572 IS -0.252 0.325 24941.828: SetIntensity called with factor 1.0008 24941.828: Changing (ideal) Record exposure by 1.0008 to 1.5939 24941.828: Frame time for constraint is 0.1598 24941.843: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 24946.671: recentlyReversed: dir 1 ang -3.998510 revtilt -1.004377 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.009 0.066 um, 0.7 4.8 % Error in Z Prediction -0.104 Predicted X = 0.064 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.394 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.579 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, 0.012 um; position 0.055, 0.381 Measured defocus = -0.06 microns changed by -0.04 to target Tracking image shifted -0.000, -0.002 um; position 0.056, 0.383 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0007_-4.0.tif Frame alignment results: distance raw = 5.3 smoothed = 5.1 Weighted resid mean = 0.04 max max = 0.08 Mean unweighted max = 0.27 Record image shifted 0.000, 0.006 um Tracking reference shifted by -1.0,-4.6 pixels, 0.000,0.006 um Saved Z = 7 at 3.31 min Tilt = -4.00 Mean = 41 Exp = 1.594 X/Y/Z = 0.055 0.377 3.536 24974.250: Saved state 7 at -4.0: def 3.436 IS -0.226 0.303 int 0.12072 exp 1.594 24974.250: SetIntensity called with factor 1.0011 24974.250: Changing (ideal) Record exposure by 1.0011 to 1.5957 24974.250: Frame time for constraint is 0.1598 24974.265: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 24977.390: recentlyReversed: dir 1 ang -1.994756 revtilt 0.990379 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.009 -0.017 um, 0.6 1.2 % Error in Z Prediction -0.043 Predicted X = 0.049 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = 0.355 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Z = 3.428 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, 0.014 um; position 0.056, 0.342 Measured defocus = -0.11 microns changed by 0.01 to target Tracking image shifted -0.000, -0.002 um; position 0.056, 0.343 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0008_-2.0.tif Frame alignment results: distance raw = 6.4 smoothed = 6.1 Weighted resid mean = 0.06 max max = 0.09 Mean unweighted max = 0.18 Record image shifted -0.000, 0.006 um Tracking reference shifted by -0.6,-4.4 pixels, -0.000,0.006 um Saved Z = 8 at 3.82 min Tilt = -1.99 Mean = 41 Exp = 1.596 X/Y/Z = 0.057 0.337 3.442 25004.843: Saved state 8 at -2.0: def 3.342 IS -0.209 0.267 int 0.12072 exp 1.596 25004.937: Restored state 6 at -18.0 for tiltInd 9 def 4.271 IS -0.254 0.287 25004.937: SetIntensity called with factor 1.0014 25004.937: Changing (ideal) Record exposure by 1.0014 to 1.5979 25004.953: Frame time for constraint is 0.1598 25004.953: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.008 -0.018 um, 0.5 1.3 % Error in Z Prediction 0.014 Predicted X = 0.056 StdErr = 0.032 nFit = 3 nDrop = 0 Predicted Y = 0.412 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Z = 4.495 StdErr = 0.079 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.015, -0.004 um; position 0.071, 0.417 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0009_-20.0.tif Frame alignment results: distance raw = 3.5 smoothed = 2.9 Weighted resid mean = 0.04 max max = 0.06 Mean unweighted max = 0.17 Record image shifted 0.002, -0.009 um Tracking reference shifted by -0.1,6.9 pixels, 0.002,-0.009 um Saved Z = 9 at 4.25 min Tilt = -19.99 Mean = 42 Exp = 1.598 X/Y/Z = 0.069 0.426 4.495 25030.250: Saved state 9 at -20.0: def 4.395 IS -0.261 0.339 int 0.12072 exp 1.598 25030.265: SetIntensity called with factor 1.0017 25030.265: Changing (ideal) Record exposure by 1.0017 to 1.6006 25030.265: Frame time for constraint is 0.1598 25030.281: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.013 0.013 um, 0.9 1.0 % Predicted X = 0.061 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = 0.480 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Z = 4.606 StdErr = 0.096 nFit = 3 nDrop = 0 Need autofocus Measured defocus = -0.12 microns changed by 0.02 to target 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0010_-22.0.tif Frame alignment results: distance raw = 4.5 smoothed = 3.4 Weighted resid mean = 0.05 max max = 0.08 Mean unweighted max = 0.22 Record image shifted -0.012, 0.043 um Saved Z = 10 at 4.66 min Tilt = -21.99 Mean = 42 Exp = 1.601 X/Y/Z = 0.073 0.437 4.623 25055.093: Saved state 10 at -22.0: def 4.523 IS -0.270 0.347 int 0.12072 exp 1.601 25055.171: Restored state 8 at -2.0 for tiltInd 11 def 3.342 IS -0.209 0.267 25055.187: SetIntensity called with factor 1.0014 25055.187: Changing (ideal) Record exposure by 1.0014 to 1.5979 25055.187: Frame time for constraint is 0.1598 25055.203: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25060.265: recentlyReversed: dir 1 ang 0.001000 revtilt 2.998133 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.012 -0.043 um, 0.9 3.1 % Error in Z Prediction 0.017 Predicted X = 0.049 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.303 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Z = 3.321 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.011, -0.045 um; position 0.039, 0.348 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0011_0.0.tif Frame alignment results: distance raw = 7.0 smoothed = 6.7 Weighted resid mean = 0.05 max max = 0.08 Mean unweighted max = 0.19 Record image shifted -0.000, 0.005 um Tracking reference shifted by -0.3,-3.6 pixels, -0.000,0.005 um Saved Z = 11 at 5.12 min Tilt = 0.00 Mean = 41 Exp = 1.598 X/Y/Z = 0.039 0.343 3.321 25082.437: Saved state 11 at 0.0: def 3.221 IS -0.196 0.282 int 0.12072 exp 1.598 25082.437: SetIntensity called with factor 1.0017 25082.453: Changing (ideal) Record exposure by 1.0017 to 1.6006 25082.453: Frame time for constraint is 0.1598 25082.453: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25085.843: recentlyReversed: dir 1 ang 1.999255 revtilt 4.998388 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.010 0.040 um, 0.7 2.9 % Predicted X = 0.034 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.318 StdErr = 0.033 nFit = 3 nDrop = 0 Predicted Z = 3.206 StdErr = 0.042 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, -0.061 um; position 0.034, 0.379 Measured defocus = -0.13 microns changed by 0.03 to target Tracking image shifted 0.000, -0.001 um; position 0.034, 0.381 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0012_2.0.tif Frame alignment results: distance raw = 10.3 smoothed = 10.0 Weighted resid mean = 0.05 max max = 0.08 Mean unweighted max = 0.21 Record image shifted 0.000, 0.005 um Tracking reference shifted by -0.8,-3.9 pixels, 0.000,0.005 um Saved Z = 12 at 5.63 min Tilt = 2.00 Mean = 41 Exp = 1.601 X/Y/Z = 0.034 0.375 3.231 25113.421: Saved state 12 at 2.0: def 3.131 IS -0.207 0.313 int 0.12072 exp 1.601 25113.500: Restored state 10 at -22.0 for tiltInd 13 def 4.523 IS -0.270 0.347 25113.500: SetIntensity called with factor 1.0020 25113.500: Changing (ideal) Record exposure by 1.0020 to 1.6039 25113.515: Frame time for constraint is 0.1598 25113.515: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.000 0.057 um, 0.0 4.1 % Error in Z Prediction 0.026 Predicted X = 0.072 StdErr = 0.025 nFit = 4 nDrop = 0 Predicted Y = 0.432 StdErr = 0.017 nFit = 4 nDrop = 0 Predicted Z = 4.715 StdErr = 0.051 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.033 um; position 0.066, 0.399 Measured defocus = -0.12 microns changed by 0.02 to target Tracking image shifted 0.000, -0.000 um; position 0.066, 0.399 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0013_-24.0.tif Frame alignment results: distance raw = 3.9 smoothed = 3.2 Weighted resid mean = 0.07 max max = 0.10 Mean unweighted max = 0.21 Record image shifted 0.002, -0.013 um Tracking reference shifted by 0.4,9.6 pixels, 0.002,-0.013 um Saved Z = 13 at 6.16 min Tilt = -23.99 Mean = 42 Exp = 1.604 X/Y/Z = 0.064 0.412 4.734 25144.890: Saved state 13 at -24.0: def 4.634 IS -0.250 0.329 int 0.12072 exp 1.604 25144.890: SetIntensity called with factor 1.0024 25144.890: Changing (ideal) Record exposure by 1.0024 to 1.6076 25144.906: Frame time for constraint is 0.1598 25144.906: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.008 -0.020 um, 0.6 1.5 % Error in Z Prediction 0.019 Predicted X = 0.068 StdErr = 0.024 nFit = 4 nDrop = 0 Predicted Y = 0.366 StdErr = 0.015 nFit = 5 nDrop = 0 Predicted Z = 4.835 StdErr = 0.034 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.009, 0.017 um; position 0.076, 0.349 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0014_-26.0.tif Frame alignment results: distance raw = 4.2 smoothed = 3.5 Weighted resid mean = 0.05 max max = 0.09 Mean unweighted max = 0.22 Record image shifted 0.001, -0.009 um Tracking reference shifted by 0.6,6.3 pixels, 0.001,-0.009 um Saved Z = 14 at 6.55 min Tilt = -25.99 Mean = 42 Exp = 1.608 X/Y/Z = 0.076 0.357 4.835 25168.203: Saved state 14 at -26.0: def 4.735 IS -0.234 0.275 int 0.12072 exp 1.608 25168.281: Restored state 12 at 2.0 for tiltInd 15 def 3.131 IS -0.207 0.313 25168.296: SetIntensity called with factor 1.0020 25168.296: Changing (ideal) Record exposure by 1.0020 to 1.6038 25168.296: Frame time for constraint is 0.1598 25168.312: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25174.250: recentlyReversed: dir 1 ang 3.999010 revtilt 6.998143 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.008 -0.009 um, 0.6 0.6 % Predicted X = 0.020 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = 0.390 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Z = 3.131 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.006, 0.000 um; position 0.015, 0.390 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0015_4.0.tif Frame alignment results: distance raw = 9.8 smoothed = 9.8 Weighted resid mean = 0.05 max max = 0.07 Mean unweighted max = 0.19 Record image shifted 0.000, 0.004 um Tracking reference shifted by -0.7,-2.8 pixels, 0.000,0.004 um Saved Z = 15 at 7.02 min Tilt = 4.00 Mean = 40 Exp = 1.604 X/Y/Z = 0.015 0.386 3.131 25196.515: Saved state 15 at 4.0: def 3.031 IS -0.195 0.333 int 0.12072 exp 1.604 25196.515: SetIntensity called with factor 1.0023 25196.531: Changing (ideal) Record exposure by 1.0023 to 1.6076 25196.531: Frame time for constraint is 0.1598 25196.546: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25199.921: recentlyReversed: dir 1 ang 5.999265 revtilt 8.998898 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.006 -0.004 um, 0.4 0.3 % Predicted X = 0.001 StdErr = 0.018 nFit = 4 nDrop = 0 Predicted Y = 0.411 StdErr = 0.033 nFit = 4 nDrop = 0 Predicted Z = 3.029 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.016, 0.029 um; position 0.017, 0.381 Measured defocus = -0.01 microns changed by -0.09 to target Tracking image shifted -0.001, -0.002 um; position 0.018, 0.383 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0016_6.0.tif Frame alignment results: distance raw = 12.3 smoothed = 12.0 Weighted resid mean = 0.07 max max = 0.10 Mean unweighted max = 0.21 Record image shifted 0.001, 0.007 um Tracking reference shifted by -1.3,-4.9 pixels, 0.001,0.007 um Saved Z = 16 at 7.53 min Tilt = 6.00 Mean = 40 Exp = 1.608 X/Y/Z = 0.017 0.377 2.941 25227.515: Saved state 16 at 6.0: def 2.841 IS -0.194 0.323 int 0.12072 exp 1.608 25227.593: Restored state 14 at -26.0 for tiltInd 17 def 4.735 IS -0.234 0.275 25227.593: SetIntensity called with factor 1.0027 25227.609: Changing (ideal) Record exposure by 1.0027 to 1.6120 25227.609: Frame time for constraint is 0.1598 25227.609: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.016 -0.034 um, 1.2 2.5 % Error in Z Prediction -0.088 Predicted X = 0.083 StdErr = 0.023 nFit = 4 nDrop = 0 Predicted Y = 0.264 StdErr = 0.004 nFit = 5 nDrop = 1 Predicted Z = 4.944 StdErr = 0.039 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, -0.027 um; position 0.070, 0.291 Measured defocus = -0.10 microns changed by -0.00 to target Tracking image shifted 0.000, -0.003 um; position 0.069, 0.294 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0017_-28.0.tif Frame alignment results: distance raw = 6.4 smoothed = 5.3 Weighted resid mean = 0.06 max max = 0.09 Mean unweighted max = 0.29 Record image shifted 0.002, -0.008 um Tracking reference shifted by -0.3,5.9 pixels, 0.002,-0.008 um Saved Z = 17 at 8.06 min Tilt = -27.99 Mean = 42 Exp = 1.612 X/Y/Z = 0.068 0.302 4.943 25259.328: Saved state 17 at -28.0: def 4.843 IS -0.201 0.231 int 0.12072 exp 1.612 25259.343: SetIntensity called with factor 1.0030 25259.343: Changing (ideal) Record exposure by 1.0030 to 1.6168 25259.359: Frame time for constraint is 0.1598 25259.359: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.015 0.038 um, 1.1 2.7 % Error in Z Prediction -0.000 Predicted X = 0.071 StdErr = 0.027 nFit = 4 nDrop = 0 Predicted Y = 0.195 StdErr = 0.019 nFit = 6 nDrop = 0 Predicted Z = 5.068 StdErr = 0.022 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, -0.130 um; position 0.072, 0.325 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0018_-30.0.tif Frame alignment results: distance raw = 4.7 smoothed = 3.4 Weighted resid mean = 0.07 max max = 0.11 Mean unweighted max = 0.26 Record image shifted 0.002, -0.012 um Tracking reference shifted by 0.4,8.7 pixels, 0.002,-0.012 um Saved Z = 18 at 8.45 min Tilt = -29.99 Mean = 42 Exp = 1.617 X/Y/Z = 0.070 0.336 5.068 25282.609: Saved state 18 at -30.0: def 4.968 IS -0.220 0.259 int 0.12072 exp 1.617 25282.687: Restored state 16 at 6.0 for tiltInd 19 def 2.841 IS -0.194 0.323 25282.703: SetIntensity called with factor 1.0027 25282.703: Changing (ideal) Record exposure by 1.0027 to 1.6119 25282.718: Frame time for constraint is 0.1598 25282.718: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25289.140: recentlyReversed: dir 1 ang 7.999020 revtilt 10.994153 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.000 0.141 um, 0.0 10.2 % Predicted X = 0.015 StdErr = 0.024 nFit = 4 nDrop = 0 Predicted Y = 0.374 StdErr = 0.027 nFit = 5 nDrop = 0 Predicted Z = 2.844 StdErr = 0.045 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.009, 0.028 um; position 0.006, 0.347 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0019_8.0.tif Frame alignment results: distance raw = 12.6 smoothed = 12.4 Weighted resid mean = 0.08 max max = 0.13 Mean unweighted max = 0.18 Record image shifted 0.000, 0.004 um Tracking reference shifted by -0.5,-2.6 pixels, 0.000,0.004 um Saved Z = 19 at 8.93 min Tilt = 8.00 Mean = 40 Exp = 1.612 X/Y/Z = 0.006 0.343 2.844 25311.359: Saved state 19 at 8.0: def 2.744 IS -0.168 0.300 int 0.12072 exp 1.612 25311.375: SetIntensity called with factor 1.0030 25311.375: Changing (ideal) Record exposure by 1.0030 to 1.6167 25311.390: Frame time for constraint is 0.1598 25311.390: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25314.796: recentlyReversed: dir 1 ang 9.999275 revtilt 12.998907 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.009 -0.031 um, 0.7 2.2 % Predicted X = 0.004 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = 0.290 StdErr = 0.002 nFit = 5 nDrop = 1 Predicted Z = 2.725 StdErr = 0.051 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.011, 0.001 um; position 0.014, 0.288 Measured defocus = -0.15 microns changed by 0.05 to target Tracking image shifted -0.001, -0.002 um; position 0.015, 0.290 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0020_10.0.tif Frame alignment results: distance raw = 14.4 smoothed = 13.7 Weighted resid mean = 0.05 max max = 0.08 Mean unweighted max = 0.23 Record image shifted 0.001, 0.005 um Tracking reference shifted by -1.2,-3.7 pixels, 0.001,0.005 um Saved Z = 20 at 9.45 min Tilt = 10.00 Mean = 39 Exp = 1.617 X/Y/Z = 0.014 0.285 2.776 25342.359: Saved state 20 at 10.0: def 2.676 IS -0.147 0.244 int 0.12072 exp 1.617 25342.437: Restored state 18 at -30.0 for tiltInd 21 def 4.968 IS -0.220 0.259 25342.437: SetIntensity called with factor 1.0034 25342.453: Changing (ideal) Record exposure by 1.0034 to 1.6223 25342.453: Frame time for constraint is 0.1598 25342.468: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.011 -0.005 um, 0.8 0.4 % Error in Z Prediction 0.051 Predicted X = 0.061 StdErr = 0.021 nFit = 4 nDrop = 0 Predicted Y = 0.287 StdErr = 0.066 nFit = 6 nDrop = 0 Predicted Z = 5.182 StdErr = 0.025 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, -0.080 um; position 0.054, 0.367 Measured defocus = -0.11 microns changed by 0.01 to target Tracking image shifted 0.001, -0.005 um; position 0.053, 0.372 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0021_-32.0.tif Frame alignment results: distance raw = 7.7 smoothed = 7.4 Weighted resid mean = 0.07 max max = 0.11 Mean unweighted max = 0.17 Record image shifted 0.002, -0.007 um Tracking reference shifted by -0.2,5.6 pixels, 0.002,-0.007 um Saved Z = 21 at 9.99 min Tilt = -31.99 Mean = 42 Exp = 1.622 X/Y/Z = 0.051 0.379 5.192 25374.765: Saved state 21 at -32.0: def 5.092 IS -0.224 0.308 int 0.12072 exp 1.622 25374.765: SetIntensity called with factor 1.0037 25374.781: Changing (ideal) Record exposure by 1.0037 to 1.6284 25374.781: Frame time for constraint is 0.1598 25374.796: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.010 0.092 um, 0.7 6.7 % Error in Z Prediction 0.010 Predicted X = 0.046 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = 0.474 StdErr = 0.034 nFit = 5 nDrop = 0 Predicted Z = 5.297 StdErr = 0.018 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.010, 0.085 um; position 0.057, 0.389 Measured defocus = 0.06 microns changed by -0.16 to target Tracking image shifted 0.000, -0.001 um; position 0.056, 0.389 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0022_-34.0.tif Frame alignment results: distance raw = 8.9 smoothed = 7.4 Weighted resid mean = 0.06 max max = 0.11 Mean unweighted max = 0.33 Record image shifted 0.001, -0.010 um Tracking reference shifted by 0.4,7.5 pixels, 0.001,-0.010 um Saved Z = 22 at 10.47 min Tilt = -33.99 Mean = 42 Exp = 1.628 X/Y/Z = 0.055 0.399 5.140 25403.781: Saved state 22 at -34.0: def 5.040 IS -0.236 0.323 int 0.12072 exp 1.628 25403.843: Restored state 20 at 10.0 for tiltInd 23 def 2.676 IS -0.147 0.244 25403.859: SetIntensity called with factor 1.0034 25403.859: Changing (ideal) Record exposure by 1.0034 to 1.6221 25403.875: Frame time for constraint is 0.1598 25403.875: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25410.734: recentlyReversed: dir 1 ang 11.999530 revtilt 14.998662 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.008 -0.074 um, 0.6 5.4 % Error in Z Prediction -0.157 Predicted X = 0.009 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = 0.207 StdErr = 0.003 nFit = 6 nDrop = 0 Predicted Z = 2.640 StdErr = 0.052 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, -0.062 um; position 0.001, 0.269 Measured defocus = -0.15 microns changed by 0.05 to target Tracking image shifted -0.001, -0.002 um; position 0.002, 0.271 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0023_12.0.tif Frame alignment results: distance raw = 15.5 smoothed = 13.8 Weighted resid mean = 0.07 max max = 0.11 Mean unweighted max = 0.22 Record image shifted 0.000, 0.005 um Tracking reference shifted by -0.9,-3.5 pixels, 0.000,0.005 um Saved Z = 23 at 11.05 min Tilt = 12.00 Mean = 39 Exp = 1.622 X/Y/Z = 0.002 0.266 2.689 25438.140: Saved state 23 at 12.0: def 2.589 IS -0.128 0.234 int 0.12072 exp 1.622 25438.140: SetIntensity called with factor 1.0037 25438.156: Changing (ideal) Record exposure by 1.0037 to 1.6282 25438.171: Frame time for constraint is 0.1598 25438.171: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25441.453: recentlyReversed: dir 1 ang 13.999285 revtilt 16.994418 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.007 0.060 um, 0.5 4.3 % Error in Z Prediction 0.049 Predicted X = 0.004 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.210 StdErr = 0.037 nFit = 5 nDrop = 0 Predicted Z = 2.563 StdErr = 0.059 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, -0.074 um; position 0.007, 0.284 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0024_14.0.tif Frame alignment results: distance raw = 18.8 smoothed = 17.6 Weighted resid mean = 0.07 max max = 0.13 Mean unweighted max = 0.34 Record image shifted 0.001, 0.007 um Tracking reference shifted by -1.5,-5.0 pixels, 0.001,0.007 um Saved Z = 24 at 11.47 min Tilt = 14.00 Mean = 39 Exp = 1.628 X/Y/Z = 0.006 0.277 2.563 25463.421: Saved state 24 at 14.0: def 2.463 IS -0.136 0.241 int 0.12072 exp 1.628 25463.500: Restored state 22 at -34.0 for tiltInd 25 def 5.040 IS -0.236 0.323 25463.515: SetIntensity called with factor 1.0041 25463.515: Changing (ideal) Record exposure by 1.0041 to 1.6351 25463.531: Frame time for constraint is 0.1598 25463.531: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.002 0.067 um, 0.2 4.8 % Predicted X = 0.043 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = 0.483 StdErr = 0.060 nFit = 5 nDrop = 0 Predicted Z = 5.295 StdErr = 0.102 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.008, 0.141 um; position 0.051, 0.342 Measured defocus = -0.16 microns changed by 0.06 to target Tracking image shifted 0.001, 0.001 um; position 0.050, 0.341 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0025_-36.0.tif Frame alignment results: distance raw = 13.7 smoothed = 12.8 Weighted resid mean = 0.10 max max = 0.16 Mean unweighted max = 0.29 Record image shifted 0.004, -0.018 um Tracking reference shifted by -0.8,13.4 pixels, 0.004,-0.018 um Saved Z = 25 at 12.02 min Tilt = -35.99 Mean = 42 Exp = 1.635 X/Y/Z = 0.046 0.359 5.356 25496.546: Saved state 25 at -36.0: def 5.256 IS -0.209 0.292 int 0.12072 exp 1.635 25496.562: SetIntensity called with factor 1.0045 25496.562: Changing (ideal) Record exposure by 1.0045 to 1.6424 25496.578: Frame time for constraint is 0.1598 25496.578: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.002 -0.124 um, 0.2 9.0 % Error in Z Prediction 0.060 Predicted X = 0.045 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.322 StdErr = 0.037 nFit = 5 nDrop = 0 Predicted Z = 5.411 StdErr = 0.113 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.016, 0.010 um; position 0.061, 0.312 Measured defocus = -0.16 microns changed by 0.06 to target Tracking image shifted -0.001, 0.001 um; position 0.062, 0.311 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0026_-38.0.tif Frame alignment results: distance raw = 12.2 smoothed = 11.6 Weighted resid mean = 0.06 max max = 0.10 Mean unweighted max = 0.39 Record image shifted 0.003, -0.014 um Tracking reference shifted by -0.2,10.3 pixels, 0.003,-0.014 um Saved Z = 26 at 12.50 min Tilt = -37.99 Mean = 41 Exp = 1.642 X/Y/Z = 0.059 0.324 5.471 25525.296: Saved state 26 at -38.0: def 5.371 IS -0.204 0.255 int 0.12072 exp 1.642 25525.390: Restored state 24 at 14.0 for tiltInd 27 def 2.463 IS -0.136 0.241 25525.390: SetIntensity called with factor 1.0041 25525.406: Changing (ideal) Record exposure by 1.0041 to 1.6348 25525.406: Frame time for constraint is 0.1598 25525.421: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25532.609: recentlyReversed: dir 1 ang 15.999040 revtilt 18.998672 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.014 0.003 um, 1.0 0.2 % Error in Z Prediction 0.060 Predicted X = -0.001 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = 0.290 StdErr = 0.033 nFit = 5 nDrop = 0 Predicted Z = 2.457 StdErr = 0.066 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.011, -0.012 um; position -0.012, 0.302 Measured defocus = -0.08 microns changed by -0.02 to target Tracking image shifted -0.001, -0.005 um; position -0.010, 0.308 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0027_16.0.tif Frame alignment results: distance raw = 14.3 smoothed = 13.0 Weighted resid mean = 0.08 max max = 0.12 Mean unweighted max = 0.27 Record image shifted 0.001, 0.011 um Tracking reference shifted by -2.2,-7.5 pixels, 0.001,0.011 um Saved Z = 27 at 13.08 min Tilt = 16.00 Mean = 38 Exp = 1.635 X/Y/Z = -0.011 0.297 2.432 25560.031: Saved state 27 at 16.0: def 2.332 IS -0.132 0.269 int 0.12072 exp 1.635 25560.046: SetIntensity called with factor 1.0045 25560.062: Changing (ideal) Record exposure by 1.0045 to 1.6421 25560.062: Frame time for constraint is 0.1598 25560.078: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25563.468: recentlyReversed: dir 1 ang 17.999795 revtilt 20.998927 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.010 0.007 um, 0.7 0.5 % Error in Z Prediction -0.025 Predicted X = -0.014 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.357 StdErr = 0.016 nFit = 5 nDrop = 0 Predicted Z = 2.355 StdErr = 0.049 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.010, 0.053 um; position -0.004, 0.305 Measured defocus = -0.10 microns changed by -0.00 to target Tracking image shifted -0.000, -0.003 um; position -0.004, 0.307 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0028_18.0.tif Frame alignment results: distance raw = 13.5 smoothed = 9.5 Weighted resid mean = 0.08 max max = 0.12 Mean unweighted max = 0.24 Record image shifted 0.001, 0.006 um Tracking reference shifted by -1.3,-4.5 pixels, 0.001,0.006 um Saved Z = 28 at 13.59 min Tilt = 18.00 Mean = 38 Exp = 1.642 X/Y/Z = -0.005 0.301 2.350 25590.906: Saved state 28 at 18.0: def 2.250 IS -0.139 0.269 int 0.12072 exp 1.642 25591.000: Restored state 26 at -38.0 for tiltInd 29 def 5.371 IS -0.204 0.255 25591.000: SetIntensity called with factor 1.0049 25591.015: Changing (ideal) Record exposure by 1.0049 to 1.6504 25591.031: Frame time for constraint is 0.1598 25591.031: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.009 -0.056 um, 0.7 4.1 % Error in Z Prediction -0.005 Predicted X = 0.057 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = 0.238 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 5.544 StdErr = 0.093 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.031 um; position 0.053, 0.270 Measured defocus = -0.32 microns changed by 0.22 to target Tracking image shifted 0.001, -0.003 um; position 0.052, 0.273 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0029_-40.0.tif Frame alignment results: distance raw = 14.4 smoothed = 13.7 Weighted resid mean = 0.08 max max = 0.14 Mean unweighted max = 0.34 Record image shifted 0.005, -0.018 um Tracking reference shifted by -1.0,13.6 pixels, 0.005,-0.018 um Saved Z = 29 at 14.15 min Tilt = -39.99 Mean = 41 Exp = 1.650 X/Y/Z = 0.047 0.291 5.767 25624.281: Saved state 29 at -40.0: def 5.667 IS -0.178 0.231 int 0.12072 exp 1.650 25624.296: SetIntensity called with factor 1.0053 25624.296: Changing (ideal) Record exposure by 1.0053 to 1.6591 25624.312: Frame time for constraint is 0.1598 25624.312: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.011 0.052 um, 0.8 3.8 % Error in Z Prediction 0.223 Predicted X = 0.045 StdErr = 0.032 nFit = 4 nDrop = 0 Predicted Y = 0.224 StdErr = 0.036 nFit = 5 nDrop = 0 Predicted Z = 5.932 StdErr = 0.083 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted -0.008, -0.065 um; position 0.054, 0.289 Measured defocus = -0.08 microns changed by -0.02 to target Tracking image shifted 0.001, -0.002 um; position 0.053, 0.291 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0030_-42.0.tif Frame alignment results: distance raw = 12.3 smoothed = 11.9 Weighted resid mean = 0.09 max max = 0.15 Mean unweighted max = 0.44 Record image shifted 0.002, -0.015 um Tracking reference shifted by 0.8,11.2 pixels, 0.002,-0.015 um Saved Z = 30 at 14.63 min Tilt = -41.99 Mean = 41 Exp = 1.659 X/Y/Z = 0.051 0.307 5.916 25653.265: Saved state 30 at -42.0: def 5.816 IS -0.189 0.243 int 0.12072 exp 1.659 25653.343: Restored state 28 at 18.0 for tiltInd 31 def 2.250 IS -0.139 0.269 25653.359: SetIntensity called with factor 1.0049 25653.375: Changing (ideal) Record exposure by 1.0049 to 1.6500 25653.375: Frame time for constraint is 0.1598 25653.390: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25660.937: recentlyReversed: dir 1 ang 19.999050 revtilt 22.998682 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.006 0.082 um, 0.4 6.0 % Error in Z Prediction -0.016 Predicted X = -0.009 StdErr = 0.029 nFit = 4 nDrop = 0 Predicted Y = 0.331 StdErr = 0.024 nFit = 5 nDrop = 0 Predicted Z = 2.229 StdErr = 0.029 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.012, 0.048 um; position -0.021, 0.284 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0031_20.0.tif Frame alignment results: distance raw = 16.3 smoothed = 15.0 Weighted resid mean = 0.10 max max = 0.16 Mean unweighted max = 0.26 Record image shifted -0.000, 0.002 um Tracking reference shifted by -0.2,-1.4 pixels, -0.000,0.002 um Saved Z = 31 at 15.13 min Tilt = 20.00 Mean = 37 Exp = 1.650 X/Y/Z = -0.021 0.282 2.229 25683.062: Saved state 31 at 20.0: def 2.129 IS -0.116 0.260 int 0.12072 exp 1.650 25683.062: SetIntensity called with factor 1.0053 25683.078: Changing (ideal) Record exposure by 1.0053 to 1.6587 25683.093: Frame time for constraint is 0.1598 25683.093: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25686.375: recentlyReversed: dir 1 ang 21.999305 revtilt 24.998437 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.012 -0.050 um, 0.9 3.6 % Predicted X = -0.022 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.263 StdErr = 0.018 nFit = 5 nDrop = 0 Predicted Z = 2.118 StdErr = 0.034 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.014, 0.015 um; position -0.008, 0.248 Measured defocus = -0.14 microns changed by 0.04 to target Tracking image shifted -0.001, -0.002 um; position -0.007, 0.250 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0032_22.0.tif Frame alignment results: distance raw = 16.7 smoothed = 15.6 Weighted resid mean = 0.07 max max = 0.13 Mean unweighted max = 0.54 Record image shifted 0.003, 0.003 um Tracking reference shifted by -2.3,-2.1 pixels, 0.003,0.003 um Saved Z = 32 at 15.64 min Tilt = 22.00 Mean = 37 Exp = 1.659 X/Y/Z = -0.009 0.247 2.153 25713.968: Saved state 32 at 22.0: def 2.053 IS -0.109 0.223 int 0.12072 exp 1.659 25714.046: Restored state 30 at -42.0 for tiltInd 33 def 5.816 IS -0.189 0.243 25714.062: SetIntensity called with factor 1.0057 25714.062: Changing (ideal) Record exposure by 1.0057 to 1.6686 25714.078: Frame time for constraint is 0.1598 25714.093: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.012 -0.016 um, 0.9 1.2 % Error in Z Prediction 0.035 Predicted X = 0.045 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = 0.317 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 6.119 StdErr = 0.063 nFit = 5 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted 0.002, 0.031 um; position 0.042, 0.286 Measured defocus = 0.10 microns changed by -0.20 to target Tracking image shifted 0.001, -0.002 um; position 0.041, 0.287 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0033_-44.0.tif Frame alignment results: distance raw = 15.1 smoothed = 14.9 Weighted resid mean = 0.14 max max = 0.20 Mean unweighted max = 0.29 Record image shifted 0.004, -0.017 um Tracking reference shifted by -0.9,12.9 pixels, 0.004,-0.017 um Saved Z = 33 at 16.21 min Tilt = -43.99 Mean = 41 Exp = 1.669 X/Y/Z = 0.037 0.304 5.918 25748.109: Saved state 33 at -44.0: def 5.818 IS -0.176 0.249 int 0.12072 exp 1.669 25748.125: SetIntensity called with factor 1.0062 25748.140: Changing (ideal) Record exposure by 1.0062 to 1.6789 25748.140: Frame time for constraint is 0.1598 25748.156: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.008 -0.012 um, 0.5 0.9 % Error in Z Prediction -0.201 Predicted X = 0.031 StdErr = 0.023 nFit = 4 nDrop = 0 Predicted Y = 0.330 StdErr = 0.019 nFit = 6 nDrop = 0 Predicted Z = 6.181 StdErr = 0.108 nFit = 6 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, 0.028 um; position 0.036, 0.301 Measured defocus = 0.15 microns changed by -0.25 to target Tracking image shifted 0.000, 0.003 um; position 0.036, 0.299 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0034_-46.0.tif Frame alignment results: distance raw = 14.9 smoothed = 13.4 Weighted resid mean = 0.12 max max = 0.19 Mean unweighted max = 0.42 Record image shifted 0.001, -0.020 um Tracking reference shifted by 1.7,14.4 pixels, 0.001,-0.020 um Saved Z = 34 at 16.70 min Tilt = -45.99 Mean = 41 Exp = 1.679 X/Y/Z = 0.035 0.318 5.929 25777.343: Saved state 34 at -46.0: def 5.829 IS -0.181 0.263 int 0.12072 exp 1.679 25777.437: Restored state 32 at 22.0 for tiltInd 35 def 2.053 IS -0.109 0.223 25777.437: SetIntensity called with factor 1.0057 25777.453: Changing (ideal) Record exposure by 1.0057 to 1.6682 25777.468: Frame time for constraint is 0.1598 25777.484: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 25785.296: recentlyReversed: dir 1 ang 23.999060 revtilt 26.998192 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.004 -0.011 um, 0.3 0.8 % Error in Z Prediction -0.252 Predicted X = -0.016 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.191 StdErr = 0.003 nFit = 5 nDrop = 1 Predicted Z = 2.035 StdErr = 0.037 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.019, 0.004 um; position -0.035, 0.187 Measured defocus = -0.12 microns changed by 0.02 to target Tracking image shifted -0.003, -0.004 um; position -0.032, 0.191 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0035_24.0.tif Frame alignment results: distance raw = 18.3 smoothed = 17.6 Weighted resid mean = 0.06 max max = 0.10 Mean unweighted max = 0.33 Record image shifted -0.003, 0.002 um Tracking reference shifted by 1.7,-2.0 pixels, -0.003,0.002 um Saved Z = 35 at 17.29 min Tilt = 24.00 Mean = 36 Exp = 1.668 X/Y/Z = -0.029 0.189 2.053 25812.953: Saved state 35 at 24.0: def 1.953 IS -0.065 0.183 int 0.12072 exp 1.668 25812.968: SetIntensity called with factor 1.0061 25812.984: Changing (ideal) Record exposure by 1.0061 to 1.6784 25812.984: Frame time for constraint is 0.1598 25813.000: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 25816.250: recentlyReversed: dir 1 ang 25.999315 revtilt 28.998447 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.013 -0.002 um, 1.0 0.2 % Error in Z Prediction 0.018 Predicted X = -0.037 StdErr = 0.039 nFit = 4 nDrop = 0 Predicted Y = 0.112 StdErr = 0.003 nFit = 5 nDrop = 0 Predicted Z = 1.960 StdErr = 0.011 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted -0.027, -0.052 um; position -0.010, 0.164 Measured defocus = -0.13 microns changed by 0.03 to target Tracking image shifted -0.002, -0.003 um; position -0.008, 0.167 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0036_26.0.tif Frame alignment results: distance raw = 18.7 smoothed = 17.7 Weighted resid mean = 0.10 max max = 0.16 Mean unweighted max = 0.36 Record image shifted 0.003, 0.003 um Tracking reference shifted by -2.5,-1.5 pixels, 0.003,0.003 um Saved Z = 36 at 17.81 min Tilt = 26.00 Mean = 35 Exp = 1.678 X/Y/Z = -0.011 0.164 1.991 25844.156: Saved state 36 at 26.0: def 1.891 IS -0.069 0.151 int 0.12072 exp 1.678 25844.234: Restored state 34 at -46.0 for tiltInd 37 def 5.829 IS -0.181 0.263 25844.250: SetIntensity called with factor 1.0066 25844.265: Changing (ideal) Record exposure by 1.0066 to 1.6901 25844.281: Frame time for constraint is 0.1598 25844.296: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.026 0.052 um, 1.9 3.8 % Error in Z Prediction 0.030 Predicted X = 0.021 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Y = 0.354 StdErr = 0.020 nFit = 6 nDrop = 0 Predicted Z = 6.004 StdErr = 0.087 nFit = 4 nDrop = 3 Need regular tracking - Need autofocus Tracking image shifted -0.017, 0.129 um; position 0.038, 0.225 Measured defocus = -0.27 microns changed by 0.17 to target Tracking image shifted 0.001, 0.003 um; position 0.038, 0.221 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0037_-48.0.tif Frame alignment results: distance raw = 21.3 smoothed = 19.9 Weighted resid mean = 0.12 max max = 0.18 Mean unweighted max = 0.48 Record image shifted 0.003, -0.013 um Tracking reference shifted by -0.5,9.7 pixels, 0.003,-0.013 um Saved Z = 37 at 18.39 min Tilt = -47.99 Mean = 40 Exp = 1.690 X/Y/Z = 0.035 0.234 6.168 25878.828: Saved state 37 at -48.0: def 6.068 IS -0.141 0.188 int 0.12072 exp 1.690 25878.828: SetIntensity called with factor 1.0071 25878.843: Changing (ideal) Record exposure by 1.0071 to 1.7021 25878.859: Frame time for constraint is 0.1598 25878.875: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.013 -0.120 um, 1.0 8.7 % Error in Z Prediction 0.163 Predicted X = 0.044 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = 0.216 StdErr = 0.060 nFit = 6 nDrop = 0 Predicted Z = 6.289 StdErr = 0.127 nFit = 7 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.006, 0.065 um; position 0.050, 0.151 Measured defocus = -0.03 microns changed by -0.07 to target Tracking image shifted -0.001, 0.007 um; position 0.051, 0.144 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0038_-50.0.tif Frame alignment results: distance raw = 19.6 smoothed = 14.1 Weighted resid mean = 0.13 max max = 0.21 Mean unweighted max = 0.73 Record image shifted 0.003, -0.018 um Tracking reference shifted by 0.4,13.6 pixels, 0.003,-0.018 um Saved Z = 38 at 18.88 min Tilt = -49.99 Mean = 40 Exp = 1.702 X/Y/Z = 0.048 0.162 6.207 25908.218: Saved state 38 at -50.0: def 6.107 IS -0.118 0.117 int 0.12072 exp 1.702 25908.312: Restored state 36 at 26.0 for tiltInd 39 def 1.891 IS -0.069 0.151 25908.328: SetIntensity called with factor 1.0066 25908.328: Changing (ideal) Record exposure by 1.0066 to 1.6895 25908.343: Frame time for constraint is 0.1598 25908.359: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 25916.531: recentlyReversed: dir 1 ang 27.999070 revtilt 30.998702 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.004 -0.054 um, 0.3 3.9 % Error in Z Prediction -0.082 Predicted X = -0.019 StdErr = 0.028 nFit = 3 nDrop = 0 Predicted Y = 0.110 StdErr = 0.029 nFit = 5 nDrop = 0 Predicted Z = 1.886 StdErr = 0.018 nFit = 5 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, -0.070 um; position -0.016, 0.180 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0039_28.0.tif Frame alignment results: distance raw = 15.9 smoothed = 14.0 Weighted resid mean = 0.12 max max = 0.18 Mean unweighted max = 0.55 Record image shifted 0.002, 0.002 um Tracking reference shifted by -1.9,-0.8 pixels, 0.002,0.002 um Saved Z = 39 at 19.39 min Tilt = 28.00 Mean = 34 Exp = 1.689 X/Y/Z = -0.018 0.178 1.886 25939.062: Saved state 39 at 28.0: def 1.786 IS -0.070 0.167 int 0.12072 exp 1.689 25939.078: SetIntensity called with factor 1.0071 25939.078: Changing (ideal) Record exposure by 1.0071 to 1.7015 25939.093: Frame time for constraint is 0.1598 25939.109: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 25942.328: recentlyReversed: dir 1 ang 29.999325 revtilt 32.998957 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.001 0.068 um, 0.1 4.9 % Predicted X = -0.008 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.159 StdErr = 0.040 nFit = 6 nDrop = 0 Predicted Z = 1.794 StdErr = 0.021 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.018 um; position -0.011, 0.177 Measured defocus = -0.06 microns changed by -0.04 to target Tracking image shifted -0.000, -0.004 um; position -0.011, 0.181 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0040_30.0.tif Frame alignment results: distance raw = 15.7 smoothed = 14.5 Weighted resid mean = 0.12 max max = 0.19 Mean unweighted max = 0.57 Record image shifted 0.001, 0.017 um Tracking reference shifted by -2.7,-12.5 pixels, 0.001,0.017 um Saved Z = 40 at 19.91 min Tilt = 30.00 Mean = 34 Exp = 1.701 X/Y/Z = -0.011 0.163 1.756 25970.062: Saved state 40 at 30.0: def 1.656 IS -0.068 0.150 int 0.12072 exp 1.701 25970.156: Restored state 38 at -50.0 for tiltInd 41 def 6.107 IS -0.118 0.117 25970.171: SetIntensity called with factor 1.0077 25970.187: Changing (ideal) Record exposure by 1.0077 to 1.7152 25970.187: Frame time for constraint is 0.1598 25970.203: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.003 0.004 um, 0.2 0.3 % Error in Z Prediction -0.038 Predicted X = 0.053 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.038 StdErr = 0.045 nFit = 5 nDrop = 0 Predicted Z = 6.281 StdErr = 0.089 nFit = 6 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.058 um; position 0.049, 0.097 Measured defocus = -0.33 microns changed by 0.23 to target Tracking image shifted -0.002, 0.003 um; position 0.051, 0.094 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0041_-52.0.tif Frame alignment results: distance raw = 28.7 smoothed = 26.8 Weighted resid mean = 0.16 max max = 0.26 Mean unweighted max = 0.53 Record image shifted 0.002, -0.021 um Tracking reference shifted by 1.4,15.4 pixels, 0.002,-0.021 um Saved Z = 41 at 20.50 min Tilt = -51.99 Mean = 40 Exp = 1.715 X/Y/Z = 0.049 0.114 6.508 26005.250: Saved state 41 at -52.0: def 6.408 IS -0.096 0.075 int 0.12072 exp 1.715 26005.265: SetIntensity called with factor 1.0082 26005.281: Changing (ideal) Record exposure by 1.0082 to 1.7293 26005.296: Frame time for constraint is 0.1598 26005.312: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.004 0.076 um, 0.3 5.5 % Error in Z Prediction 0.227 Predicted X = 0.056 StdErr = 0.018 nFit = 4 nDrop = 0 Predicted Y = -0.003 StdErr = 0.050 nFit = 6 nDrop = 0 Predicted Z = 6.514 StdErr = 0.140 nFit = 6 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.015, -0.042 um; position 0.072, 0.039 Measured defocus = -0.18 microns changed by 0.08 to target Tracking image shifted -0.003, 0.003 um; position 0.074, 0.036 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0042_-54.0.tif Frame alignment results: distance raw = 14.1 smoothed = 13.0 Weighted resid mean = 0.11 max max = 0.17 Mean unweighted max = 0.47 Record image shifted 0.003, -0.023 um Tracking reference shifted by 1.3,17.1 pixels, 0.003,-0.023 um Saved Z = 42 at 20.98 min Tilt = -53.98 Mean = 40 Exp = 1.729 X/Y/Z = 0.072 0.059 6.594 26034.281: Saved state 42 at -54.0: def 6.494 IS -0.089 0.013 int 0.12072 exp 1.729 26034.375: Restored state 40 at 30.0 for tiltInd 43 def 1.656 IS -0.068 0.150 26034.390: SetIntensity called with factor 1.0076 26034.406: Changing (ideal) Record exposure by 1.0076 to 1.7144 26034.421: Frame time for constraint is 0.1598 26034.421: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 26042.906: recentlyReversed: dir 1 ang 31.999080 revtilt 34.994213 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.015 0.062 um, 1.1 4.5 % Error in Z Prediction 0.080 Predicted X = -0.013 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.197 StdErr = 0.036 nFit = 5 nDrop = 0 Predicted Z = 1.672 StdErr = 0.026 nFit = 5 nDrop = 0 Need regular tracking - Tracking image shifted -0.031, 0.049 um; position 0.018, 0.148 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0043_32.0.tif Frame alignment results: distance raw = 11.9 smoothed = 10.1 Weighted resid mean = 0.11 max max = 0.19 Mean unweighted max = 0.53 Record image shifted 0.000, -0.002 um Tracking reference shifted by 0.1,1.6 pixels, 0.000,-0.002 um Saved Z = 43 at 21.50 min Tilt = 32.00 Mean = 33 Exp = 1.714 X/Y/Z = 0.018 0.150 1.672 26065.421: Saved state 43 at 32.0: def 1.572 IS -0.086 0.123 int 0.12072 exp 1.714 26065.437: SetIntensity called with factor 1.0082 26065.453: Changing (ideal) Record exposure by 1.0082 to 1.7284 26065.468: Frame time for constraint is 0.1598 26065.484: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 26068.750: recentlyReversed: dir 1 ang 33.999835 revtilt 36.994468 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.031 -0.047 um, 2.2 3.4 % Predicted X = 0.031 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = 0.142 StdErr = 0.027 nFit = 5 nDrop = 0 Predicted Z = 1.574 StdErr = 0.029 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.027 um; position 0.030, 0.115 Measured defocus = -0.20 microns changed by 0.10 to target Tracking image shifted -0.000, -0.005 um; position 0.030, 0.120 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0044_34.0.tif Frame alignment results: distance raw = 9.8 smoothed = 8.5 Weighted resid mean = 0.10 max max = 0.16 Mean unweighted max = 0.23 Record image shifted 0.003, 0.003 um Tracking reference shifted by -2.8,-1.4 pixels, 0.003,0.003 um Saved Z = 44 at 22.02 min Tilt = 34.00 Mean = 32 Exp = 1.728 X/Y/Z = 0.027 0.118 1.667 26096.640: Saved state 44 at 34.0: def 1.567 IS -0.079 0.090 int 0.12072 exp 1.728 26096.718: Restored state 42 at -54.0 for tiltInd 45 def 6.494 IS -0.089 0.013 26096.734: SetIntensity called with factor 1.0088 26096.750: Changing (ideal) Record exposure by 1.0088 to 1.7446 26096.765: Frame time for constraint is 0.1598 26096.781: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.004 -0.025 um, 0.3 1.8 % Error in Z Prediction 0.093 Predicted X = 0.090 StdErr = 0.024 nFit = 4 nDrop = 0 Predicted Y = 0.027 StdErr = 0.014 nFit = 5 nDrop = 1 Predicted Z = 6.736 StdErr = 0.101 nFit = 6 nDrop = 1 Need autofocus Measured defocus = -0.32 microns changed by 0.22 to target 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0045_-56.0.tif Frame alignment results: distance raw = 10.4 smoothed = 9.5 Weighted resid mean = 0.09 max max = 0.15 Mean unweighted max = 0.43 Record image shifted 0.003, -0.057 um Saved Z = 45 at 22.57 min Tilt = -55.98 Mean = 39 Exp = 1.745 X/Y/Z = 0.087 0.084 6.958 26129.937: Saved state 45 at -56.0: def 6.858 IS -0.114 0.027 int 0.12072 exp 1.745 26129.953: SetIntensity called with factor 1.0095 26129.968: Changing (ideal) Record exposure by 1.0095 to 1.7611 26129.984: Frame time for constraint is 0.1598 26130.000: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.003 0.057 um, 0.2 4.1 % Error in Z Prediction 0.222 Predicted X = 0.108 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = 0.103 StdErr = 0.038 nFit = 5 nDrop = 0 Predicted Z = 7.011 StdErr = 0.129 nFit = 7 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, 0.071 um; position 0.110, 0.032 Measured defocus = -0.03 microns changed by -0.07 to target Tracking image shifted -0.004, 0.001 um; position 0.113, 0.031 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0046_-58.0.tif Frame alignment results: distance raw = 15.1 smoothed = 14.3 Weighted resid mean = 0.15 max max = 0.22 Mean unweighted max = 0.34 Record image shifted 0.007, -0.047 um Tracking reference shifted by 1.3,35.3 pixels, 0.007,-0.047 um Saved Z = 46 at 23.06 min Tilt = -57.99 Mean = 39 Exp = 1.761 X/Y/Z = 0.106 0.078 6.934 26159.281: Saved state 46 at -58.0: def 6.834 IS -0.128 0.011 int 0.12072 exp 1.761 26159.375: Restored state 44 at 34.0 for tiltInd 47 def 1.567 IS -0.079 0.090 26159.390: SetIntensity called with factor 1.0088 26159.406: Changing (ideal) Record exposure by 1.0088 to 1.7436 26159.421: Frame time for constraint is 0.1598 26159.437: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 26168.156: recentlyReversed: dir 1 ang 35.999090 revtilt 38.981724 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.001 -0.025 um, 0.1 1.8 % Error in Z Prediction -0.077 Predicted X = 0.049 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.073 StdErr = 0.012 nFit = 5 nDrop = 0 Predicted Z = 1.560 StdErr = 0.063 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.042, -0.006 um; position 0.091, 0.079 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0047_36.0.tif Frame alignment results: distance raw = 12.1 smoothed = 11.0 Weighted resid mean = 0.11 max max = 0.17 Mean unweighted max = 0.41 Record image shifted -0.003, -0.002 um Tracking reference shifted by 2.3,1.2 pixels, -0.003,-0.002 um Saved Z = 47 at 23.58 min Tilt = 36.00 Mean = 30 Exp = 1.744 X/Y/Z = 0.094 0.081 1.560 26190.500: Saved state 47 at 36.0: def 1.460 IS -0.119 0.021 int 0.12072 exp 1.744 26190.515: SetIntensity called with factor 1.0094 26190.531: Changing (ideal) Record exposure by 1.0094 to 1.7600 26190.546: Frame time for constraint is 0.1598 26190.562: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 26193.765: recentlyReversed: dir 1 ang 37.999345 revtilt 40.998477 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.045 0.008 um, 3.2 0.6 % Predicted X = 0.123 StdErr = 0.043 nFit = 3 nDrop = 0 Predicted Y = 0.035 StdErr = 0.008 nFit = 5 nDrop = 0 Predicted Z = 1.479 StdErr = 0.071 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.035 um; position 0.117, 0.070 Measured defocus = -0.04 microns changed by -0.06 to target Tracking image shifted -0.003, -0.011 um; position 0.120, 0.082 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0048_38.0.tif Frame alignment results: distance raw = 9.4 smoothed = 8.1 Weighted resid mean = 0.10 max max = 0.18 Mean unweighted max = 0.70 Record image shifted 0.003, 0.013 um Tracking reference shifted by -3.9,-8.9 pixels, 0.003,0.013 um Saved Z = 48 at 24.10 min Tilt = 38.00 Mean = 29 Exp = 1.760 X/Y/Z = 0.117 0.069 1.412 26221.593: Saved state 48 at 38.0: def 1.312 IS -0.132 -0.002 int 0.12072 exp 1.760 26221.687: Restored state 46 at -58.0 for tiltInd 49 def 6.834 IS -0.128 0.011 26221.703: SetIntensity called with factor 1.0101 26221.718: Changing (ideal) Record exposure by 1.0101 to 1.7790 26221.734: Frame time for constraint is 0.1598 26221.750: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.006 0.033 um, 0.4 2.4 % Error in Z Prediction -0.066 Predicted X = 0.121 StdErr = 0.008 nFit = 4 nDrop = 0 Predicted Y = 0.122 StdErr = 0.042 nFit = 5 nDrop = 0 Predicted Z = 7.179 StdErr = 0.131 nFit = 6 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.107, 0.080 um; position 0.228, 0.041 Measured defocus = 0.21 microns changed by -0.31 to target Tracking image shifted -0.002, 0.009 um; position 0.229, 0.032 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0049_-60.0.tif Frame alignment results: distance raw = 5.2 smoothed = 3.7 Weighted resid mean = 0.12 max max = 0.18 Mean unweighted max = 0.44 Record image shifted 0.015, -0.042 um Tracking reference shifted by -5.4,32.4 pixels, 0.015,-0.042 um Saved Z = 49 at 24.69 min Tilt = -59.99 Mean = 38 Exp = 1.779 X/Y/Z = 0.214 0.074 6.855 26257.156: Saved state 49 at -60.0: def 6.755 IS -0.218 -0.050 int 0.12072 exp 1.779 26257.171: SetIntensity called with factor 1.0109 26257.187: Changing (ideal) Record exposure by 1.0109 to 1.7984 26257.203: Frame time for constraint is 0.1598 26257.218: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.093 -0.048 um, 6.8 3.4 % Error in Z Prediction -0.324 Predicted X = 0.263 StdErr = 0.066 nFit = 3 nDrop = 0 Predicted Y = 0.112 StdErr = 0.038 nFit = 6 nDrop = 0 Predicted Z = 7.164 StdErr = 0.208 nFit = 6 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.033, 0.135 um; position 0.229, -0.023 Measured defocus = -0.04 microns changed by -0.06 to target Tracking image shifted 0.001, 0.009 um; position 0.229, -0.032 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0050_-62.0.tif Frame alignment results: distance raw = 6.7 smoothed = 5.7 Weighted resid mean = 0.12 max max = 0.19 Mean unweighted max = 0.49 Record image shifted 0.006, -0.035 um Tracking reference shifted by 0.5,26.5 pixels, 0.006,-0.035 um Saved Z = 50 at 25.18 min Tilt = -61.99 Mean = 37 Exp = 1.798 X/Y/Z = 0.223 0.003 7.092 26286.453: Saved state 50 at -62.0: def 6.992 IS -0.192 -0.117 int 0.12072 exp 1.798 26286.531: Restored state 48 at 38.0 for tiltInd 51 def 1.312 IS -0.132 -0.002 26286.562: SetIntensity called with factor 1.0101 26286.578: Changing (ideal) Record exposure by 1.0101 to 1.7778 26286.593: Frame time for constraint is 0.1598 26286.609: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 26295.609: recentlyReversed: dir 1 ang 39.999100 revtilt 42.985734 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.040 -0.108 um, 2.9 7.8 % Error in Z Prediction -0.073 Predicted X = 0.169 StdErr = 0.033 nFit = 3 nDrop = 0 Predicted Y = 0.037 StdErr = 0.023 nFit = 5 nDrop = 0 Predicted Z = 1.354 StdErr = 0.107 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.022, -0.034 um; position 0.191, 0.071 Measured defocus = 0.04 microns changed by -0.14 to target Tracking image shifted -0.001, -0.010 um; position 0.192, 0.081 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0051_40.0.tif Frame alignment results: distance raw = 7.4 smoothed = 6.0 Weighted resid mean = 0.14 max max = 0.21 Mean unweighted max = 0.42 Record image shifted 0.007, 0.030 um Tracking reference shifted by -9.1,-20.8 pixels, 0.007,0.030 um Saved Z = 51 at 25.80 min Tilt = 40.00 Mean = 28 Exp = 1.778 X/Y/Z = 0.185 0.051 1.206 26323.453: Saved state 51 at 40.0: def 1.106 IS -0.182 -0.054 int 0.12072 exp 1.778 26323.468: SetIntensity called with factor 1.0108 26323.484: Changing (ideal) Record exposure by 1.0108 to 1.7970 26323.500: Frame time for constraint is 0.1598 26323.515: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 26326.750: recentlyReversed: dir 1 ang 41.999355 revtilt 44.993488 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.016 0.014 um, 1.1 1.0 % Error in Z Prediction -0.148 Predicted X = 0.231 StdErr = 0.059 nFit = 4 nDrop = 0 Predicted Y = 0.046 StdErr = 0.011 nFit = 5 nDrop = 0 Predicted Z = 1.158 StdErr = 0.115 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.040, 0.007 um; position 0.191, 0.040 Measured defocus = -0.16 microns changed by 0.06 to target Tracking image shifted 0.000, -0.009 um; position 0.191, 0.048 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0052_42.0.tif Frame alignment results: distance raw = 7.4 smoothed = 5.8 Weighted resid mean = 0.13 max max = 0.20 Mean unweighted max = 0.65 Trimming A by 28 41, reference by 21 26 Record image shifted 0.003, 0.008 um Tracking reference shifted by -3.2,-5.2 pixels, 0.003,0.008 um Saved Z = 52 at 26.32 min Tilt = 42.00 Mean = 26 Exp = 1.797 X/Y/Z = 0.188 0.041 1.212 26354.546: Saved state 52 at 42.0: def 1.112 IS -0.180 -0.065 int 0.12072 exp 1.797 26354.640: Restored state 50 at -62.0 for tiltInd 53 def 6.992 IS -0.192 -0.117 26354.656: SetIntensity called with factor 1.0117 26354.671: Changing (ideal) Record exposure by 1.0117 to 1.8195 26354.687: Frame time for constraint is 0.1598 26354.703: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.043 -0.006 um, 3.1 0.4 % Error in Z Prediction 0.054 Predicted X = 0.298 StdErr = 0.074 nFit = 3 nDrop = 0 Predicted Y = -0.071 StdErr = 0.028 nFit = 5 nDrop = 0 Predicted Z = 7.192 StdErr = 0.171 nFit = 6 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.079, 0.029 um; position 0.219, -0.101 Measured defocus = -0.29 microns changed by 0.19 to target Tracking image shifted 0.000, 0.004 um; position 0.219, -0.105 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0053_-64.0.tif Frame alignment results: distance raw = 9.7 smoothed = 9.5 Weighted resid mean = 0.15 max max = 0.24 Mean unweighted max = 0.63 Record image shifted 0.011, -0.052 um Tracking reference shifted by -1.0,39.3 pixels, 0.011,-0.052 um Saved Z = 53 at 26.92 min Tilt = -63.99 Mean = 37 Exp = 1.820 X/Y/Z = 0.208 -0.053 7.368 26390.593: Saved state 53 at -64.0: def 7.268 IS -0.152 -0.159 int 0.12072 exp 1.820 26390.609: SetIntensity called with factor 1.0126 26390.625: Changing (ideal) Record exposure by 1.0126 to 1.8425 26390.640: Frame time for constraint is 0.1598 26390.656: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.090 0.018 um, 6.5 1.3 % Error in Z Prediction 0.176 Predicted X = 0.209 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = -0.151 StdErr = 0.030 nFit = 5 nDrop = 0 Predicted Z = 7.386 StdErr = 0.191 nFit = 6 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.013, 0.020 um; position 0.221, -0.171 Measured defocus = -0.27 microns changed by 0.17 to target Tracking image shifted -0.000, 0.002 um; position 0.222, -0.174 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0054_-66.0.tif Frame alignment results: distance raw = 15.0 smoothed = 14.9 Weighted resid mean = 0.20 max max = 0.34 Mean unweighted max = 0.87 Record image shifted 0.005, -0.023 um Tracking reference shifted by -0.9,17.2 pixels, 0.005,-0.023 um Saved Z = 54 at 27.42 min Tilt = -65.99 Mean = 36 Exp = 1.842 X/Y/Z = 0.216 -0.151 7.553 26420.593: Saved state 54 at -66.0: def 7.453 IS -0.113 -0.250 int 0.12072 exp 1.842 26420.687: Restored state 52 at 42.0 for tiltInd 55 def 1.112 IS -0.180 -0.065 26420.703: SetIntensity called with factor 1.0117 26420.718: Changing (ideal) Record exposure by 1.0117 to 1.8180 26420.734: Frame time for constraint is 0.1598 26420.750: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 26430.046: recentlyReversed: dir 1 ang 43.999110 revtilt 46.998242 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.008 0.000 um, 0.6 0.0 % Error in Z Prediction 0.167 Predicted X = 0.234 StdErr = 0.048 nFit = 3 nDrop = 0 Predicted Y = 0.040 StdErr = 0.012 nFit = 5 nDrop = 0 Predicted Z = 1.035 StdErr = 0.097 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.068, 0.009 um; position 0.166, 0.031 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0055_44.0.tif Frame alignment results: distance raw = 7.6 smoothed = 6.9 Weighted resid mean = 0.15 max max = 0.27 Mean unweighted max = 0.58 Trimming A by 36 46, reference by 36 36 Record image shifted 0.003, 0.015 um Tracking reference shifted by -3.9,-10.5 pixels, 0.003,0.015 um Saved Z = 55 at 27.95 min Tilt = 44.00 Mean = 24 Exp = 1.818 X/Y/Z = 0.164 0.016 1.035 26452.375: Saved state 55 at 44.0: def 0.935 IS -0.147 -0.074 int 0.12072 exp 1.818 26452.390: SetIntensity called with factor 1.0126 26452.406: Changing (ideal) Record exposure by 1.0126 to 1.8408 26452.421: Frame time for constraint is 0.1598 26452.437: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) 26455.625: recentlyReversed: dir 1 ang 45.999364 revtilt 48.998497 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.071 -0.024 um, 5.1 1.7 % Predicted X = 0.158 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Y = -0.005 StdErr = 0.008 nFit = 5 nDrop = 1 Predicted Z = 0.912 StdErr = 0.113 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.013, -0.021 um; position 0.171, 0.016 Measured defocus = 0.19 microns changed by -0.29 to target Tracking image shifted -0.001, -0.007 um; position 0.172, 0.023 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0056_46.0.tif Frame alignment results: distance raw = 8.2 smoothed = 6.1 Weighted resid mean = 0.18 max max = 0.29 Mean unweighted max = 0.61 Trimming A by 46 56, reference by 41 46 Record image shifted 0.003, 0.015 um Tracking reference shifted by -4.0,-10.7 pixels, 0.003,0.015 um Saved Z = 56 at 28.47 min Tilt = 46.00 Mean = 23 Exp = 1.841 X/Y/Z = 0.170 0.008 0.618 26483.796: Saved state 56 at 46.0: def 0.518 IS -0.149 -0.084 int 0.12072 exp 1.841 26483.890: Restored state 54 at -66.0 for tiltInd 57 def 7.453 IS -0.113 -0.250 26483.906: SetIntensity called with factor 1.0136 26483.921: Changing (ideal) Record exposure by 1.0136 to 1.8675 26483.937: Frame time for constraint is 0.1598 26483.953: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.012 0.013 um, 0.9 1.0 % Error in Z Prediction -0.294 Predicted X = 0.209 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = -0.272 StdErr = 0.030 nFit = 5 nDrop = 0 Predicted Z = 7.809 StdErr = 0.043 nFit = 4 nDrop = 2 Need regular tracking - Need autofocus Tracking image shifted 0.017, -0.039 um; position 0.192, -0.233 Measured defocus = -0.04 microns changed by -0.06 to target Tracking image shifted -0.002, 0.001 um; position 0.193, -0.235 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0057_-68.0.tif Frame alignment results: distance raw = 15.9 smoothed = 15.7 Weighted resid mean = 0.19 max max = 0.30 Mean unweighted max = 0.84 Trimming A by 21 26, reference by 21 21 Record image shifted 0.011, -0.031 um Tracking reference shifted by -3.8,24.4 pixels, 0.011,-0.031 um Saved Z = 57 at 29.08 min Tilt = -67.99 Mean = 35 Exp = 1.868 X/Y/Z = 0.182 -0.203 7.746 26520.625: Saved state 57 at -68.0: def 7.646 IS -0.059 -0.278 int 0.12072 exp 1.868 26520.640: SetIntensity called with factor 1.0147 26520.656: Changing (ideal) Record exposure by 1.0147 to 1.8949 26520.671: Frame time for constraint is 0.1598 26520.703: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) The stage is not at the expected tilt angle (at -69.89, expected -70.00, error 0.11). TILT SERIES CONTINUING WITH THIS ERROR MESSAGE: The stage is not at the expected tilt angle (at -69.89, expected -70.00, error 0.11). This is probably not due to a pole touch. Press: "Stop && Set Limit" to stop and set this as a limiting ('pole touch') angle, "Go On" to try to go on. Errors in X/Y Prediction -0.027 0.069 um, 1.9 5.0 % Error in Z Prediction -0.063 Predicted X = 0.156 StdErr = 0.044 nFit = 4 nDrop = 0 Predicted Y = -0.314 StdErr = 0.041 nFit = 6 nDrop = 0 Predicted Z = 7.984 StdErr = 0.046 nFit = 5 nDrop = 1 Need regular tracking - Tracking image shifted -0.032, -0.031 um; position 0.188, -0.283 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0058_-69.9.tif Frame alignment results: distance raw = 13.2 smoothed = 12.5 Weighted resid mean = 0.23 max max = 0.37 Mean unweighted max = 0.60 Trimming A by 28 111, reference by 21 31 Record image shifted 0.004, -0.049 um Tracking reference shifted by 4.0,36.2 pixels, 0.004,-0.049 um Saved Z = 58 at 29.58 min Tilt = -69.89 Mean = 34 Exp = 1.895 X/Y/Z = 0.184 -0.234 7.984 26550.437: Saved state 58 at -69.9: def 7.884 IS -0.046 -0.306 int 0.12072 exp 1.895 26550.531: Restored state 56 at 46.0 for tiltInd 59 def 0.518 IS -0.149 -0.084 26550.546: SetIntensity called with factor 1.0135 26550.562: Changing (ideal) Record exposure by 1.0135 to 1.8657 26550.578: Frame time for constraint is 0.1598 26550.593: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) 26560.093: recentlyReversed: dir 1 ang 47.999119 revtilt 50.998252 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.029 0.080 um, 2.1 5.8 % Predicted X = 0.155 StdErr = 0.023 nFit = 3 nDrop = 0 Predicted Y = -0.007 StdErr = 0.012 nFit = 5 nDrop = 0 Predicted Z = 0.554 StdErr = 0.161 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, 0.049 um; position 0.141, -0.056 Measured defocus = 0.07 microns changed by -0.17 to target Tracking image shifted -0.002, -0.001 um; position 0.144, -0.055 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0059_48.0.tif Frame alignment results: distance raw = 5.8 smoothed = 4.8 Weighted resid mean = 0.24 max max = 0.37 Mean unweighted max = 0.87 Trimming A by 61 61, reference by 51 56 Record image shifted 0.005, 0.007 um Tracking reference shifted by -4.3,-4.4 pixels, 0.005,0.007 um Saved Z = 59 at 30.21 min Tilt = 48.00 Mean = 21 Exp = 1.866 X/Y/Z = 0.139 -0.062 0.385 26588.359: Saved state 59 at 48.0: def 0.285 IS -0.089 -0.130 int 0.12072 exp 1.866 26588.375: SetIntensity called with factor 1.0146 26588.390: Changing (ideal) Record exposure by 1.0146 to 1.8930 26588.421: Frame time for constraint is 0.1598 26588.437: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) 26592.140: recentlyReversed: dir 1 ang 49.998874 revtilt 52.998507 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.016 -0.055 um, 1.2 3.9 % Error in Z Prediction -0.169 Predicted X = 0.122 StdErr = 0.044 nFit = 4 nDrop = 0 Predicted Y = -0.102 StdErr = 0.029 nFit = 6 nDrop = 0 Predicted Z = 0.219 StdErr = 0.148 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.033, 0.020 um; position 0.155, -0.122 Measured defocus = -2.27 microns changed by 2.17 to target Measured defocus = -0.41 microns changed by 0.31 to target Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -0.15 microns changed by 0.05 to target Tracking image shifted -0.002, 0.001 um; position 0.158, -0.123 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004_0060_50.0.tif Frame alignment results: distance raw = 7.4 smoothed = 7.0 Weighted resid mean = 0.33 max max = 0.55 Mean unweighted max = 1.45 Trimming A by 61 71, reference by 56 66 Record image shifted 0.004, 0.005 um Tracking reference shifted by -3.6,-2.8 pixels, 0.004,0.005 um Saved Z = 60 at 30.86 min Tilt = 50.00 Mean = 19 Exp = 1.893 X/Y/Z = 0.154 -0.128 2.750 2021/11/17 20:17 W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_004.mrc 0 61 11520 8184 11502 -10.0 50.0 2.00 REG 30.9 1x1 Continuum_K3 26000 1 0.17 LD-DS 20.0 0.0 Tilt series acquired at item # 32 with label 53 (item at -200.55, 332.71, TS at -200.45, 334.02) ################# PostTomo Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully ################# PostTomo END Focus area changed from stored position for item, axis position -4.00 angle 0, subarea offset 0 0