17-Nov-21 21:31:45 Trimming A by 45 50, reference by 47 50 Trimming A by 45 50, reference by 45 50 Trimming A by 40 55, reference by 45 50 Trimming A by 38 60, reference by 45 50 Rough eucentricity: changing Z by -0.98 to -7.40, finished. 31135.015: (Initial) alignment to map 42 (70) 31135.015: Target X, Y: 329.554 -2.367 31135.015: Map matches low dose View; staying in low dose but with exp 0.405 intensity 0.19459 bin 4 Trimming A by 30 20 60 20, reference by 45 20 75 20 Disparity in stage position after aligning to center of map frame: -0.50 0.15 Aligning to target with image shift equivalent to stage shift: -0.50 0.14 31142.484: Map matches low dose Preview; staying in low dose but with exp 0.202 intensity 0.12072 bin 4 31154.609: Realigned to map item itself with image shift of -2.9 -26.3 pixels Disparity in stage position after aligning to map item itself: -0.50 0.16 (align shift 0.01 -0.04) Finished aligning to item in 2 rounds Opened new file W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009.mrc STARTING TILT SERIES WITH AUTOMATIC TERMINATION AFTER ERRORS. 31162.515: Skipping reverse tilt: dir -1 start 0.00 revang 3.00 revtilt 2.99 maxtilt 69.90 maxerr 0.10 Trimming A by 70 70, reference by 20 20 Trimming A by 20 20, reference by 70 70 Measured defocus = -9.57 microns changed by 9.47 to target Measured defocus = 0.43 microns changed by -0.53 to target 31197.203: SetIntensity called with factor 0.9962 31197.203: Changing (ideal) Record exposure by 0.9962 to 1.5918 31197.203: Frame time for constraint is 0.1598 31197.203: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31197.203: Skipping reverse tilt: dir -1 start -10.00 revang -7.00 revtilt 2.99 maxtilt 69.90 maxerr 0.10 Need regular tracking - Need autofocus Tracking image shifted 0.015, 0.023 um; position 0.537, 0.085 Measured defocus = -0.01 microns changed by -0.09 to target Tracking image shifted 0.007, -0.003 um; position 0.530, 0.089 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0000_-10.0.tif Frame alignment results: distance raw = 16.7 smoothed = 16.3 Weighted resid mean = 0.08 max max = 0.12 Mean unweighted max = 0.31 Saved Z = 0 at 0.00 min Tilt = -10.00 Mean = 45 Exp = 1.592 X/Y/Z = 0.530 0.089 3.957 31216.937: Saved state 0 at -10.0: def 3.857 IS -0.494 -0.207 int 0.12072 exp 1.592 31216.937: SetIntensity called with factor 1.0002 31216.937: Changing (ideal) Record exposure by 1.0002 to 1.5920 31216.937: Frame time for constraint is 0.1598 31216.953: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Need regular tracking - Need autofocus Tracking image shifted -0.012, 0.041 um; position 0.542, 0.048 Measured defocus = -0.13 microns changed by 0.03 to target Tracking image shifted -0.002, -0.000 um; position 0.545, 0.048 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0001_-12.0.tif Frame alignment results: distance raw = 8.1 smoothed = 8.0 Weighted resid mean = 0.06 max max = 0.10 Mean unweighted max = 0.25 Record image shifted -0.003, -0.012 um Tracking reference shifted by 3.6,8.5 pixels, -0.003,-0.012 um Saved Z = 1 at 0.47 min Tilt = -11.99 Mean = 45 Exp = 1.592 X/Y/Z = 0.547 0.060 3.989 31245.156: Saved state 1 at -12.0: def 3.889 IS -0.495 -0.242 int 0.12072 exp 1.592 31245.156: SetIntensity called with factor 1.0005 31245.156: Changing (ideal) Record exposure by 1.0005 to 1.5927 31245.156: Frame time for constraint is 0.1598 31245.156: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Predicted X = 0.565 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.032 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 4.021 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, -0.004 um; position 0.558, 0.036 Measured defocus = -0.16 microns changed by 0.06 to target Tracking image shifted -0.003, -0.000 um; position 0.561, 0.036 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0002_-14.0.tif Frame alignment results: distance raw = 10.8 smoothed = 10.7 Weighted resid mean = 0.06 max max = 0.09 Mean unweighted max = 0.22 Record image shifted -0.001, -0.009 um Tracking reference shifted by 1.7,6.3 pixels, -0.001,-0.009 um Saved Z = 2 at 0.94 min Tilt = -13.99 Mean = 45 Exp = 1.593 X/Y/Z = 0.562 0.045 4.085 31273.609: Saved state 2 at -14.0: def 3.985 IS -0.500 -0.263 int 0.12072 exp 1.593 31273.687: Restored state 0 at -10.0 for tiltInd 3 def 3.857 IS -0.494 -0.207 31273.687: SetIntensity called with factor 1.0002 31273.703: Changing (ideal) Record exposure by 1.0002 to 1.5920 31273.703: Frame time for constraint is 0.1598 31273.703: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31277.687: recentlyReversed: dir 1 ang -7.995021 revtilt -5.002887 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.003 0.013 um, 0.2 1.0 % Error in Z Prediction 0.064 Need regular tracking - Need autofocus Tracking image shifted 0.011, -0.107 um; position 0.519, 0.196 Measured defocus = 0.07 microns changed by -0.17 to target Tracking image shifted -0.002, -0.001 um; position 0.521, 0.197 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0003_-8.0.tif Frame alignment results: distance raw = 6.4 smoothed = 6.2 Weighted resid mean = 0.07 max max = 0.10 Mean unweighted max = 0.22 Record image shifted -0.006, 0.003 um Tracking reference shifted by 4.1,-2.8 pixels, -0.006,0.003 um Saved Z = 3 at 1.47 min Tilt = -8.00 Mean = 45 Exp = 1.592 X/Y/Z = 0.527 0.194 3.783 31305.281: Saved state 3 at -8.0: def 3.683 IS -0.542 -0.113 int 0.12072 exp 1.592 31305.296: SetIntensity called with factor 1.0005 31305.296: Changing (ideal) Record exposure by 1.0005 to 1.5927 31305.296: Frame time for constraint is 0.1598 31305.296: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31308.687: recentlyReversed: dir 1 ang -5.999265 revtilt -3.000632 back 3.000000 3mte 0.300000 Need regular tracking - Need autofocus Tracking image shifted -0.000, -0.003 um; position 0.527, 0.197 Measured defocus = -0.03 microns changed by -0.07 to target Tracking image shifted -0.001, -0.001 um; position 0.529, 0.198 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0004_-6.0.tif Frame alignment results: distance raw = 4.1 smoothed = 3.7 Weighted resid mean = 0.05 max max = 0.09 Mean unweighted max = 0.15 Record image shifted 0.001, 0.006 um Tracking reference shifted by -1.6,-4.5 pixels, 0.001,0.006 um Saved Z = 4 at 1.99 min Tilt = -6.00 Mean = 45 Exp = 1.593 X/Y/Z = 0.527 0.192 3.717 31336.140: Saved state 4 at -6.0: def 3.617 IS -0.541 -0.115 int 0.12072 exp 1.593 31336.218: Restored state 2 at -14.0 for tiltInd 5 def 3.985 IS -0.500 -0.263 31336.218: SetIntensity called with factor 1.0008 31336.218: Changing (ideal) Record exposure by 1.0008 to 1.5940 31336.234: Frame time for constraint is 0.1598 31336.234: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Predicted X = 0.578 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.021 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Z = 4.139 StdErr = 0.048 nFit = 3 nDrop = 0 Need autofocus Measured defocus = -0.27 microns changed by 0.17 to target 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0005_-16.0.tif Frame alignment results: distance raw = 7.2 smoothed = 6.8 Weighted resid mean = 0.05 max max = 0.08 Mean unweighted max = 0.23 Record image shifted 0.030, -0.059 um Saved Z = 5 at 2.48 min Tilt = -15.99 Mean = 45 Exp = 1.594 X/Y/Z = 0.548 0.080 4.307 31365.531: Saved state 5 at -16.0: def 4.207 IS -0.505 -0.225 int 0.12072 exp 1.594 31365.531: SetIntensity called with factor 1.0011 31365.531: Changing (ideal) Record exposure by 1.0011 to 1.5957 31365.546: Frame time for constraint is 0.1598 31365.546: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.030 0.059 um, 2.2 4.3 % Error in Z Prediction 0.169 Predicted X = 0.553 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Y = 0.081 StdErr = 0.037 nFit = 3 nDrop = 0 Predicted Z = 4.446 StdErr = 0.094 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, -0.040 um; position 0.548, 0.120 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0006_-18.0.tif Frame alignment results: distance raw = 14.4 smoothed = 13.0 Weighted resid mean = 0.08 max max = 0.13 Mean unweighted max = 0.39 Record image shifted 0.007, -0.022 um Tracking reference shifted by -2.2,17.2 pixels, 0.007,-0.022 um Saved Z = 6 at 2.86 min Tilt = -17.99 Mean = 45 Exp = 1.596 X/Y/Z = 0.541 0.143 4.446 31388.734: Saved state 6 at -18.0: def 4.346 IS -0.529 -0.166 int 0.12072 exp 1.596 31388.812: Restored state 4 at -6.0 for tiltInd 7 def 3.617 IS -0.541 -0.115 31388.812: SetIntensity called with factor 1.0008 31388.828: Changing (ideal) Record exposure by 1.0008 to 1.5940 31388.828: Frame time for constraint is 0.1598 31388.828: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31393.281: recentlyReversed: dir 1 ang -3.997010 revtilt -1.004377 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.012 0.062 um, 0.8 4.5 % Predicted X = 0.528 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.190 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.650 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.021, -0.003 um; position 0.506, 0.193 Measured defocus = -0.09 microns changed by -0.01 to target Tracking image shifted 0.001, -0.001 um; position 0.506, 0.194 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0007_-4.0.tif Frame alignment results: distance raw = 6.6 smoothed = 5.9 Weighted resid mean = 0.06 max max = 0.10 Mean unweighted max = 0.17 Record image shifted 0.002, 0.014 um Tracking reference shifted by -3.3,-9.7 pixels, 0.002,0.014 um Saved Z = 7 at 3.40 min Tilt = -4.00 Mean = 45 Exp = 1.594 X/Y/Z = 0.504 0.181 3.640 31420.828: Saved state 7 at -4.0: def 3.540 IS -0.515 -0.112 int 0.12072 exp 1.594 31420.828: SetIntensity called with factor 1.0011 31420.828: Changing (ideal) Record exposure by 1.0011 to 1.5957 31420.828: Frame time for constraint is 0.1598 31420.843: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31423.968: recentlyReversed: dir 1 ang -1.997255 revtilt 0.998378 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.024 -0.009 um, 1.7 0.7 % Error in Z Prediction -0.010 Predicted X = 0.496 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.175 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Z = 3.571 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, 0.038 um; position 0.499, 0.138 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0008_-2.0.tif Frame alignment results: distance raw = 7.0 smoothed = 6.1 Weighted resid mean = 0.05 max max = 0.08 Mean unweighted max = 0.21 Record image shifted -0.002, 0.009 um Tracking reference shifted by 0.1,-6.4 pixels, -0.002,0.009 um Saved Z = 8 at 3.82 min Tilt = -2.00 Mean = 45 Exp = 1.596 X/Y/Z = 0.501 0.129 3.571 31446.203: Saved state 8 at -2.0: def 3.471 IS -0.489 -0.156 int 0.12072 exp 1.596 31446.281: Restored state 6 at -18.0 for tiltInd 9 def 4.346 IS -0.529 -0.166 31446.281: SetIntensity called with factor 1.0014 31446.281: Changing (ideal) Record exposure by 1.0014 to 1.5979 31446.281: Frame time for constraint is 0.1598 31446.296: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.005 -0.046 um, 0.4 3.3 % Predicted X = 0.530 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = 0.187 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Z = 4.605 StdErr = 0.124 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.022, 0.020 um; position 0.508, 0.167 Measured defocus = -0.09 microns changed by -0.01 to target Tracking image shifted 0.000, -0.002 um; position 0.508, 0.168 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0009_-20.0.tif Frame alignment results: distance raw = 9.5 smoothed = 9.8 Weighted resid mean = 0.06 max max = 0.09 Mean unweighted max = 0.28 Record image shifted 0.003, -0.012 um Tracking reference shifted by -0.6,9.0 pixels, 0.003,-0.012 um Saved Z = 9 at 4.33 min Tilt = -19.99 Mean = 45 Exp = 1.598 X/Y/Z = 0.505 0.180 4.597 31476.937: Saved state 9 at -20.0: def 4.497 IS -0.516 -0.113 int 0.12072 exp 1.598 31476.953: SetIntensity called with factor 1.0017 31476.953: Changing (ideal) Record exposure by 1.0017 to 1.6006 31476.953: Frame time for constraint is 0.1598 31476.968: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.025 -0.007 um, 1.8 0.5 % Error in Z Prediction -0.008 Predicted X = 0.488 StdErr = 0.022 nFit = 3 nDrop = 0 Predicted Y = 0.235 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Z = 4.774 StdErr = 0.070 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.019, 0.065 um; position 0.508, 0.169 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0010_-22.0.tif Frame alignment results: distance raw = 5.0 smoothed = 5.0 Weighted resid mean = 0.05 max max = 0.08 Mean unweighted max = 0.18 Record image shifted 0.002, -0.012 um Tracking reference shifted by 0.4,9.1 pixels, 0.002,-0.012 um Saved Z = 10 at 4.72 min Tilt = -21.99 Mean = 45 Exp = 1.601 X/Y/Z = 0.506 0.182 4.774 31500.421: Saved state 10 at -22.0: def 4.674 IS -0.518 -0.112 int 0.12072 exp 1.601 31500.500: Restored state 8 at -2.0 for tiltInd 11 def 3.471 IS -0.489 -0.156 31500.500: SetIntensity called with factor 1.0014 31500.500: Changing (ideal) Record exposure by 1.0014 to 1.5979 31500.515: Frame time for constraint is 0.1598 31500.515: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31505.562: recentlyReversed: dir 1 ang 0.002000 revtilt 2.997633 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.018 -0.053 um, 1.3 3.8 % Predicted X = 0.484 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.105 StdErr = 0.030 nFit = 3 nDrop = 0 Predicted Z = 3.499 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, -0.030 um; position 0.471, 0.135 Measured defocus = -0.11 microns changed by 0.01 to target Tracking image shifted 0.000, -0.001 um; position 0.471, 0.136 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0011_0.0.tif Frame alignment results: distance raw = 5.4 smoothed = 4.3 Weighted resid mean = 0.05 max max = 0.09 Mean unweighted max = 0.21 Record image shifted 0.000, 0.013 um Tracking reference shifted by -2.1,-9.6 pixels, 0.000,0.013 um Saved Z = 11 at 5.27 min Tilt = 0.00 Mean = 45 Exp = 1.598 X/Y/Z = 0.471 0.123 3.504 31533.046: Saved state 11 at 0.0: def 3.404 IS -0.460 -0.145 int 0.12072 exp 1.598 31533.046: SetIntensity called with factor 1.0017 31533.046: Changing (ideal) Record exposure by 1.0017 to 1.6006 31533.062: Frame time for constraint is 0.1598 31533.062: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31536.421: recentlyReversed: dir 1 ang 1.999755 revtilt 4.989889 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.014 0.018 um, 1.0 1.3 % Error in Z Prediction 0.005 Predicted X = 0.459 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Y = 0.087 StdErr = 0.033 nFit = 3 nDrop = 0 Predicted Z = 3.433 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.000, -0.090 um; position 0.459, 0.177 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0012_2.0.tif Frame alignment results: distance raw = 6.3 smoothed = 5.8 Weighted resid mean = 0.05 max max = 0.08 Mean unweighted max = 0.26 Record image shifted -0.002, 0.008 um Tracking reference shifted by 0.1,-5.9 pixels, -0.002,0.008 um Saved Z = 12 at 5.69 min Tilt = 2.00 Mean = 45 Exp = 1.601 X/Y/Z = 0.460 0.169 3.433 31558.609: Saved state 12 at 2.0: def 3.333 IS -0.473 -0.099 int 0.12072 exp 1.601 31558.703: Restored state 10 at -22.0 for tiltInd 13 def 4.674 IS -0.518 -0.112 31558.703: SetIntensity called with factor 1.0020 31558.703: Changing (ideal) Record exposure by 1.0020 to 1.6039 31558.718: Frame time for constraint is 0.1598 31558.718: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.001 0.082 um, 0.1 6.0 % Predicted X = 0.494 StdErr = 0.045 nFit = 4 nDrop = 0 Predicted Y = 0.155 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Z = 4.941 StdErr = 0.085 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, 0.020 um; position 0.485, 0.135 Measured defocus = 0.05 microns changed by -0.15 to target Tracking image shifted -0.000, -0.001 um; position 0.486, 0.136 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0013_-24.0.tif Frame alignment results: distance raw = 7.6 smoothed = 7.3 Weighted resid mean = 0.06 max max = 0.10 Mean unweighted max = 0.21 Record image shifted 0.002, -0.012 um Tracking reference shifted by -0.1,8.8 pixels, 0.002,-0.012 um Saved Z = 13 at 6.22 min Tilt = -23.99 Mean = 45 Exp = 1.604 X/Y/Z = 0.483 0.147 4.790 31590.187: Saved state 13 at -24.0: def 4.690 IS -0.482 -0.130 int 0.12072 exp 1.604 31590.203: SetIntensity called with factor 1.0024 31590.203: Changing (ideal) Record exposure by 1.0024 to 1.6076 31590.203: Frame time for constraint is 0.1598 31590.218: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.011 -0.008 um, 0.8 0.5 % Error in Z Prediction -0.151 Predicted X = 0.483 StdErr = 0.040 nFit = 4 nDrop = 0 Predicted Y = 0.083 StdErr = 0.006 nFit = 5 nDrop = 0 Predicted Z = 4.964 StdErr = 0.089 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.011, 0.007 um; position 0.494, 0.076 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0014_-26.0.tif Frame alignment results: distance raw = 4.3 smoothed = 3.9 Weighted resid mean = 0.05 max max = 0.09 Mean unweighted max = 0.18 Record image shifted 0.002, -0.015 um Tracking reference shifted by 0.9,10.9 pixels, 0.002,-0.015 um Saved Z = 14 at 6.61 min Tilt = -25.99 Mean = 45 Exp = 1.608 X/Y/Z = 0.492 0.091 4.964 31613.609: Saved state 14 at -26.0: def 4.864 IS -0.463 -0.185 int 0.12072 exp 1.608 31613.687: Restored state 12 at 2.0 for tiltInd 15 def 3.333 IS -0.473 -0.099 31613.703: SetIntensity called with factor 1.0020 31613.703: Changing (ideal) Record exposure by 1.0020 to 1.6038 31613.718: Frame time for constraint is 0.1598 31613.718: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31619.640: recentlyReversed: dir 1 ang 3.999010 revtilt 6.998643 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.009 0.007 um, 0.6 0.5 % Predicted X = 0.437 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = 0.180 StdErr = 0.039 nFit = 3 nDrop = 0 Predicted Z = 3.362 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, -0.030 um; position 0.423, 0.210 Measured defocus = -0.03 microns changed by -0.07 to target Tracking image shifted 0.000, -0.002 um; position 0.423, 0.212 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0015_4.0.tif Frame alignment results: distance raw = 4.2 smoothed = 3.1 Weighted resid mean = 0.05 max max = 0.09 Mean unweighted max = 0.22 Record image shifted -0.000, 0.011 um Tracking reference shifted by -1.3,-7.9 pixels, -0.000,0.011 um Saved Z = 15 at 7.17 min Tilt = 4.00 Mean = 45 Exp = 1.604 X/Y/Z = 0.423 0.201 3.292 31647.250: Saved state 15 at 4.0: def 3.192 IS -0.456 -0.050 int 0.12072 exp 1.604 31647.250: SetIntensity called with factor 1.0023 31647.265: Changing (ideal) Record exposure by 1.0023 to 1.6076 31647.265: Frame time for constraint is 0.1598 31647.265: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31650.625: recentlyReversed: dir 1 ang 5.999765 revtilt 8.994398 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.014 0.021 um, 1.0 1.5 % Error in Z Prediction -0.071 Predicted X = 0.394 StdErr = 0.031 nFit = 4 nDrop = 0 Predicted Y = 0.269 StdErr = 0.044 nFit = 4 nDrop = 0 Predicted Z = 3.217 StdErr = 0.049 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.023, 0.060 um; position 0.417, 0.208 Measured defocus = -0.02 microns changed by -0.08 to target Tracking image shifted 0.001, -0.003 um; position 0.416, 0.211 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0016_6.0.tif Frame alignment results: distance raw = 4.4 smoothed = 2.9 Weighted resid mean = 0.04 max max = 0.07 Mean unweighted max = 0.19 Record image shifted -0.001, 0.008 um Tracking reference shifted by -0.2,-5.7 pixels, -0.001,0.008 um Saved Z = 16 at 7.69 min Tilt = 6.00 Mean = 45 Exp = 1.608 X/Y/Z = 0.417 0.203 3.140 31678.171: Saved state 16 at 6.0: def 3.040 IS -0.452 -0.045 int 0.12072 exp 1.608 31678.250: Restored state 14 at -26.0 for tiltInd 17 def 4.864 IS -0.463 -0.185 31678.265: SetIntensity called with factor 1.0027 31678.265: Changing (ideal) Record exposure by 1.0027 to 1.6120 31678.281: Frame time for constraint is 0.1598 31678.281: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.023 -0.065 um, 1.7 4.7 % Error in Z Prediction -0.077 Predicted X = 0.491 StdErr = 0.036 nFit = 4 nDrop = 0 Predicted Y = -0.003 StdErr = 0.006 nFit = 6 nDrop = 0 Predicted Z = 5.103 StdErr = 0.100 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.032, -0.066 um; position 0.459, 0.063 Measured defocus = 0.02 microns changed by -0.12 to target Tracking image shifted 0.001, -0.004 um; position 0.458, 0.067 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0017_-28.0.tif Frame alignment results: distance raw = 7.7 smoothed = 7.4 Weighted resid mean = 0.05 max max = 0.09 Mean unweighted max = 0.22 Record image shifted 0.001, -0.011 um Tracking reference shifted by 0.6,8.1 pixels, 0.001,-0.011 um Saved Z = 17 at 8.22 min Tilt = -27.99 Mean = 44 Exp = 1.612 X/Y/Z = 0.457 0.078 4.985 31710.515: Saved state 17 at -28.0: def 4.885 IS -0.427 -0.177 int 0.12072 exp 1.612 31710.531: SetIntensity called with factor 1.0030 31710.531: Changing (ideal) Record exposure by 1.0030 to 1.6168 31710.531: Frame time for constraint is 0.1598 31710.546: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.034 0.081 um, 2.5 5.9 % Error in Z Prediction -0.118 Predicted X = 0.434 StdErr = 0.050 nFit = 4 nDrop = 0 Predicted Y = -0.006 StdErr = 0.041 nFit = 6 nDrop = 0 Predicted Z = 5.115 StdErr = 0.053 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.025, -0.104 um; position 0.459, 0.098 Measured defocus = -0.07 microns changed by -0.03 to target Tracking image shifted 0.001, -0.003 um; position 0.458, 0.100 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0018_-30.0.tif Frame alignment results: distance raw = 8.1 smoothed = 7.8 Weighted resid mean = 0.07 max max = 0.11 Mean unweighted max = 0.23 Record image shifted 0.002, -0.016 um Tracking reference shifted by 1.0,11.6 pixels, 0.002,-0.016 um Saved Z = 18 at 8.71 min Tilt = -29.99 Mean = 44 Exp = 1.617 X/Y/Z = 0.457 0.116 5.083 31739.328: Saved state 18 at -30.0: def 4.983 IS -0.445 -0.144 int 0.12072 exp 1.617 31739.390: Restored state 16 at 6.0 for tiltInd 19 def 3.040 IS -0.452 -0.045 31739.406: SetIntensity called with factor 1.0027 31739.406: Changing (ideal) Record exposure by 1.0027 to 1.6119 31739.421: Frame time for constraint is 0.1598 31739.421: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31745.734: recentlyReversed: dir 1 ang 7.999520 revtilt 10.997653 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.022 0.123 um, 1.6 8.9 % Error in Z Prediction -0.032 Predicted X = 0.399 StdErr = 0.038 nFit = 4 nDrop = 0 Predicted Y = 0.235 StdErr = 0.045 nFit = 5 nDrop = 0 Predicted Z = 3.072 StdErr = 0.058 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.037, 0.066 um; position 0.362, 0.169 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0019_8.0.tif Frame alignment results: distance raw = 3.9 smoothed = 3.2 Weighted resid mean = 0.04 max max = 0.07 Mean unweighted max = 0.22 Record image shifted -0.001, 0.006 um Tracking reference shifted by 0.1,-4.8 pixels, -0.001,0.006 um Saved Z = 19 at 9.18 min Tilt = 8.00 Mean = 45 Exp = 1.612 X/Y/Z = 0.363 0.162 3.072 31767.875: Saved state 19 at 8.0: def 2.972 IS -0.387 -0.052 int 0.12072 exp 1.612 31767.890: SetIntensity called with factor 1.0030 31767.890: Changing (ideal) Record exposure by 1.0030 to 1.6167 31767.906: Frame time for constraint is 0.1598 31767.906: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31771.140: recentlyReversed: dir 1 ang 9.999275 revtilt 12.981410 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.035 -0.072 um, 2.6 5.2 % Predicted X = 0.321 StdErr = 0.052 nFit = 4 nDrop = 0 Predicted Y = 0.104 StdErr = 0.015 nFit = 5 nDrop = 1 Predicted Z = 2.973 StdErr = 0.065 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.036, -0.011 um; position 0.357, 0.115 Measured defocus = -0.10 microns changed by -0.00 to target Tracking image shifted 0.000, -0.001 um; position 0.356, 0.116 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0020_10.0.tif Frame alignment results: distance raw = 6.9 smoothed = 5.2 Weighted resid mean = 0.06 max max = 0.09 Mean unweighted max = 0.21 Record image shifted -0.001, 0.007 um Tracking reference shifted by -0.3,-5.3 pixels, -0.001,0.007 um Saved Z = 20 at 9.69 min Tilt = 10.00 Mean = 45 Exp = 1.617 X/Y/Z = 0.357 0.109 2.970 31798.640: Saved state 20 at 10.0: def 2.870 IS -0.357 -0.097 int 0.12072 exp 1.617 31798.718: Restored state 18 at -30.0 for tiltInd 21 def 4.983 IS -0.445 -0.144 31798.718: SetIntensity called with factor 1.0034 31798.734: Changing (ideal) Record exposure by 1.0034 to 1.6223 31798.750: Frame time for constraint is 0.1598 31798.750: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.036 0.005 um, 2.6 0.4 % Error in Z Prediction -0.004 Predicted X = 0.432 StdErr = 0.052 nFit = 4 nDrop = 0 Predicted Y = 0.157 StdErr = 0.041 nFit = 5 nDrop = 1 Predicted Z = 5.180 StdErr = 0.001 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.040, 0.065 um; position 0.392, 0.092 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0021_-32.0.tif Frame alignment results: distance raw = 4.6 smoothed = 4.2 Weighted resid mean = 0.05 max max = 0.09 Mean unweighted max = 0.26 Record image shifted 0.002, -0.015 um Tracking reference shifted by 0.5,11.2 pixels, 0.002,-0.015 um Saved Z = 21 at 10.15 min Tilt = -31.99 Mean = 44 Exp = 1.622 X/Y/Z = 0.390 0.107 5.180 31825.796: Saved state 21 at -32.0: def 5.080 IS -0.383 -0.115 int 0.12072 exp 1.622 31825.796: SetIntensity called with factor 1.0037 31825.812: Changing (ideal) Record exposure by 1.0037 to 1.6284 31825.812: Frame time for constraint is 0.1598 31825.828: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.043 -0.049 um, 3.1 3.6 % Predicted X = 0.367 StdErr = 0.050 nFit = 3 nDrop = 0 Predicted Y = 0.157 StdErr = 0.038 nFit = 6 nDrop = 0 Predicted Z = 5.278 StdErr = 0.001 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.029, 0.061 um; position 0.396, 0.095 Measured defocus = -0.16 microns changed by 0.06 to target Tracking image shifted 0.001, -0.000 um; position 0.394, 0.096 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0022_-34.0.tif Frame alignment results: distance raw = 9.5 smoothed = 9.0 Weighted resid mean = 0.07 max max = 0.11 Mean unweighted max = 0.30 Record image shifted 0.001, -0.016 um Tracking reference shifted by 1.2,11.7 pixels, 0.001,-0.016 um Saved Z = 22 at 10.63 min Tilt = -33.99 Mean = 43 Exp = 1.628 X/Y/Z = 0.393 0.111 5.339 31854.500: Saved state 22 at -34.0: def 5.239 IS -0.388 -0.113 int 0.12072 exp 1.628 31854.578: Restored state 20 at 10.0 for tiltInd 23 def 2.870 IS -0.357 -0.097 31854.578: SetIntensity called with factor 1.0034 31854.593: Changing (ideal) Record exposure by 1.0034 to 1.6221 31854.593: Frame time for constraint is 0.1598 31854.609: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31861.281: recentlyReversed: dir 1 ang 11.999030 revtilt 14.993163 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.026 -0.045 um, 1.9 3.3 % Error in Z Prediction 0.061 Predicted X = 0.320 StdErr = 0.036 nFit = 3 nDrop = 0 Predicted Y = 0.021 StdErr = 0.011 nFit = 6 nDrop = 0 Predicted Z = 2.846 StdErr = 0.039 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.010, -0.066 um; position 0.310, 0.087 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0023_12.0.tif Frame alignment results: distance raw = 4.2 smoothed = 2.0 Weighted resid mean = 0.07 max max = 0.11 Mean unweighted max = 0.18 Record image shifted -0.002, 0.006 um Tracking reference shifted by 0.4,-4.3 pixels, -0.002,0.006 um Saved Z = 23 at 11.11 min Tilt = 12.00 Mean = 45 Exp = 1.622 X/Y/Z = 0.311 0.081 2.846 31883.453: Saved state 23 at 12.0: def 2.746 IS -0.304 -0.096 int 0.12072 exp 1.622 31883.468: SetIntensity called with factor 1.0037 31883.484: Changing (ideal) Record exposure by 1.0037 to 1.6282 31883.484: Frame time for constraint is 0.1598 31883.500: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31886.703: recentlyReversed: dir 1 ang 13.999285 revtilt 16.998417 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.009 0.061 um, 0.6 4.4 % Predicted X = 0.290 StdErr = 0.058 nFit = 4 nDrop = 0 Predicted Y = -0.008 StdErr = 0.035 nFit = 6 nDrop = 0 Predicted Z = 2.737 StdErr = 0.045 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.013, -0.104 um; position 0.304, 0.096 Measured defocus = -0.09 microns changed by -0.01 to target Tracking image shifted 0.001, -0.001 um; position 0.303, 0.097 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0024_14.0.tif Frame alignment results: distance raw = 4.4 smoothed = 3.3 Weighted resid mean = 0.06 max max = 0.09 Mean unweighted max = 0.22 Record image shifted -0.001, 0.005 um Tracking reference shifted by 0.4,-3.9 pixels, -0.001,0.005 um Saved Z = 24 at 11.62 min Tilt = 14.00 Mean = 44 Exp = 1.628 X/Y/Z = 0.304 0.092 2.723 31914.203: Saved state 24 at 14.0: def 2.623 IS -0.303 -0.083 int 0.12072 exp 1.628 31914.281: Restored state 22 at -34.0 for tiltInd 25 def 5.239 IS -0.388 -0.113 31914.312: SetIntensity called with factor 1.0041 31914.312: Changing (ideal) Record exposure by 1.0041 to 1.6350 31914.328: Frame time for constraint is 0.1598 31914.328: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.014 0.100 um, 1.0 7.2 % Error in Z Prediction -0.015 Predicted X = 0.350 StdErr = 0.053 nFit = 3 nDrop = 0 Predicted Y = 0.116 StdErr = 0.037 nFit = 5 nDrop = 0 Predicted Z = 5.432 StdErr = 0.032 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, 0.077 um; position 0.346, 0.038 Measured defocus = -0.12 microns changed by 0.02 to target Tracking image shifted 0.001, -0.001 um; position 0.345, 0.039 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0025_-36.0.tif Frame alignment results: distance raw = 11.7 smoothed = 11.5 Weighted resid mean = 0.08 max max = 0.13 Mean unweighted max = 0.28 Record image shifted 0.001, -0.014 um Tracking reference shifted by 1.4,10.1 pixels, 0.001,-0.014 um Saved Z = 25 at 12.17 min Tilt = -35.99 Mean = 43 Exp = 1.635 X/Y/Z = 0.344 0.053 5.455 31947.250: Saved state 25 at -36.0: def 5.355 IS -0.318 -0.139 int 0.12072 exp 1.635 31947.265: SetIntensity called with factor 1.0045 31947.281: Changing (ideal) Record exposure by 1.0045 to 1.6424 31947.281: Frame time for constraint is 0.1598 31947.296: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.006 -0.063 um, 0.4 4.5 % Error in Z Prediction 0.023 Predicted X = 0.330 StdErr = 0.039 nFit = 3 nDrop = 0 Predicted Y = -0.014 StdErr = 0.028 nFit = 5 nDrop = 0 Predicted Z = 5.575 StdErr = 0.016 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.029, -0.017 um; position 0.359, 0.004 Measured defocus = 0.64 microns changed by -0.74 to target Tracking image shifted -0.004, -0.005 um; position 0.362, 0.008 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0026_-38.0.tif Frame alignment results: distance raw = 20.4 smoothed = 20.3 Weighted resid mean = 0.19 max max = 0.34 Mean unweighted max = 0.73 Record image shifted -0.004, -0.013 um Tracking reference shifted by 4.6,9.1 pixels, -0.004,-0.013 um Saved Z = 26 at 12.70 min Tilt = -37.99 Mean = 42 Exp = 1.642 X/Y/Z = 0.366 0.022 4.838 31979.046: Saved state 26 at -38.0: def 4.738 IS -0.323 -0.178 int 0.12072 exp 1.642 31979.125: Restored state 24 at 14.0 for tiltInd 27 def 2.623 IS -0.303 -0.083 31979.140: SetIntensity called with factor 1.0041 31979.140: Changing (ideal) Record exposure by 1.0041 to 1.6348 31979.156: Frame time for constraint is 0.1598 31979.156: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 31986.218: recentlyReversed: dir 1 ang 15.999040 revtilt 18.998672 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.036 0.035 um, 2.6 2.5 % Error in Z Prediction -0.737 Predicted X = 0.271 StdErr = 0.030 nFit = 3 nDrop = 0 Predicted Y = 0.103 StdErr = 0.027 nFit = 5 nDrop = 0 Predicted Z = 2.620 StdErr = 0.040 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.047, -0.021 um; position 0.318, 0.124 Measured defocus = -0.04 microns changed by -0.06 to target Tracking image shifted -0.009, -0.002 um; position 0.328, 0.125 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0027_16.0.tif Frame alignment results: distance raw = 19.4 smoothed = 19.4 Weighted resid mean = 0.08 max max = 0.15 Mean unweighted max = 0.39 Record image shifted -0.016, 0.002 um Tracking reference shifted by 11.5,-3.8 pixels, -0.016,0.002 um Saved Z = 27 at 13.28 min Tilt = 16.00 Mean = 44 Exp = 1.635 X/Y/Z = 0.344 0.123 2.562 32013.781: Saved state 27 at 16.0: def 2.462 IS -0.352 -0.077 int 0.12072 exp 1.635 32013.781: SetIntensity called with factor 1.0045 32013.796: Changing (ideal) Record exposure by 1.0045 to 1.6421 32013.796: Frame time for constraint is 0.1598 32013.812: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 32017.015: recentlyReversed: dir 1 ang 17.999295 revtilt 20.998427 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.073 0.020 um, 5.3 1.5 % Error in Z Prediction -0.058 Predicted X = 0.353 StdErr = 0.035 nFit = 3 nDrop = 0 Predicted Y = 0.188 StdErr = 0.006 nFit = 5 nDrop = 1 Predicted Z = 2.475 StdErr = 0.054 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.040, 0.028 um; position 0.393, 0.161 Measured defocus = -0.20 microns changed by 0.10 to target Tracking image shifted -0.013, -0.009 um; position 0.406, 0.170 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0028_18.0.tif Frame alignment results: distance raw = 32.7 smoothed = 32.5 Weighted resid mean = 0.11 max max = 0.20 Mean unweighted max = 0.39 Record image shifted -0.008, -0.007 um Tracking reference shifted by 6.9,4.1 pixels, -0.008,-0.007 um Saved Z = 28 at 13.79 min Tilt = 18.00 Mean = 44 Exp = 1.642 X/Y/Z = 0.414 0.177 2.574 32044.437: Saved state 28 at 18.0: def 2.474 IS -0.437 -0.066 int 0.12072 exp 1.642 32044.515: Restored state 26 at -38.0 for tiltInd 29 def 4.738 IS -0.323 -0.178 32044.531: SetIntensity called with factor 1.0048 32044.531: Changing (ideal) Record exposure by 1.0048 to 1.6503 32044.546: Frame time for constraint is 0.1598 32044.562: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.061 -0.011 um, 4.4 0.8 % Error in Z Prediction 0.099 Predicted X = 0.341 StdErr = 0.053 nFit = 3 nDrop = 0 Predicted Y = -0.045 StdErr = 0.034 nFit = 5 nDrop = 0 Predicted Z = 5.197 StdErr = 0.397 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.245, 0.013 um; position 0.586, -0.058 Measured defocus = -0.49 microns changed by 0.39 to target Tracking image shifted -0.012, 0.000 um; position 0.598, -0.058 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0029_-40.0.tif Frame alignment results: distance raw = 20.7 smoothed = 20.7 Weighted resid mean = 0.36 max max = 0.57 Mean unweighted max = 0.94 Record image shifted -0.010, -0.019 um Tracking reference shifted by 9.7,12.2 pixels, -0.010,-0.019 um Saved Z = 29 at 14.35 min Tilt = -39.99 Mean = 41 Exp = 1.650 X/Y/Z = 0.608 -0.039 5.583 32078.000: Saved state 29 at -40.0: def 5.483 IS -0.500 -0.362 int 0.12072 exp 1.650 32078.015: SetIntensity called with factor 1.0053 32078.031: Changing (ideal) Record exposure by 1.0053 to 1.6591 32078.031: Frame time for constraint is 0.1598 32078.046: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.267 0.005 um, 19.3 0.4 % Error in Z Prediction 0.386 Predicted X = 0.703 StdErr = 0.164 nFit = 3 nDrop = 0 Predicted Y = -0.116 StdErr = 0.034 nFit = 5 nDrop = 0 Predicted Z = 5.405 StdErr = 0.454 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.044, -0.070 um; position 0.659, -0.046 Measured defocus = -0.45 microns changed by 0.35 to target Tracking image shifted -0.005, -0.003 um; position 0.663, -0.043 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0030_-42.0.tif Frame alignment results: distance raw = 15.0 smoothed = 14.9 Weighted resid mean = 0.60 max max = 1.10 Mean unweighted max = 1.42 Record image shifted 0.010, -0.018 um Tracking reference shifted by -5.1,14.8 pixels, 0.010,-0.018 um Saved Z = 30 at 14.83 min Tilt = -41.99 Mean = 40 Exp = 1.659 X/Y/Z = 0.653 -0.024 5.755 32107.062: Saved state 30 at -42.0: def 5.655 IS -0.545 -0.373 int 0.12072 exp 1.659 32107.140: Restored state 28 at 18.0 for tiltInd 31 def 2.474 IS -0.437 -0.066 32107.156: SetIntensity called with factor 1.0049 32107.156: Changing (ideal) Record exposure by 1.0049 to 1.6501 32107.171: Frame time for constraint is 0.1598 32107.187: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 32114.609: recentlyReversed: dir 1 ang 19.999050 revtilt 22.992683 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.050 0.092 um, 3.6 6.6 % Error in Z Prediction 0.350 Predicted X = 0.464 StdErr = 0.023 nFit = 3 nDrop = 0 Predicted Y = 0.262 StdErr = 0.009 nFit = 5 nDrop = 0 Predicted Z = 2.420 StdErr = 0.068 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.133, 0.093 um; position 0.597, 0.169 Measured defocus = -0.01 microns changed by -0.09 to target Tracking image shifted 0.001, -0.001 um; position 0.595, 0.170 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0031_20.0.tif Frame alignment results: distance raw = 16.0 smoothed = 15.2 Weighted resid mean = 0.08 max max = 0.14 Mean unweighted max = 0.44 Record image shifted 0.021, 0.011 um Tracking reference shifted by -16.5,-5.1 pixels, 0.021,0.011 um Saved Z = 31 at 15.42 min Tilt = 20.00 Mean = 44 Exp = 1.650 X/Y/Z = 0.575 0.160 2.327 32142.234: Saved state 31 at 20.0: def 2.227 IS -0.566 -0.169 int 0.12072 exp 1.650 32142.250: SetIntensity called with factor 1.0053 32142.250: Changing (ideal) Record exposure by 1.0053 to 1.6587 32142.265: Frame time for constraint is 0.1598 32142.281: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 32145.500: recentlyReversed: dir 1 ang 21.999305 revtilt 24.993438 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.111 -0.103 um, 8.0 7.4 % Error in Z Prediction -0.093 Predicted X = 0.675 StdErr = 0.068 nFit = 3 nDrop = 0 Predicted Y = 0.182 StdErr = 0.053 nFit = 5 nDrop = 0 Predicted Z = 2.248 StdErr = 0.084 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.099, 0.062 um; position 0.576, 0.121 Measured defocus = -0.17 microns changed by 0.07 to target Tracking image shifted 0.003, -0.003 um; position 0.573, 0.123 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0032_22.0.tif Frame alignment results: distance raw = 17.3 smoothed = 16.7 Weighted resid mean = 0.10 max max = 0.16 Mean unweighted max = 0.40 Record image shifted 0.000, -0.004 um Tracking reference shifted by 0.5,3.0 pixels, 0.000,-0.004 um Saved Z = 32 at 15.94 min Tilt = 22.00 Mean = 44 Exp = 1.659 X/Y/Z = 0.573 0.127 2.315 32173.421: Saved state 32 at 22.0: def 2.215 IS -0.549 -0.196 int 0.12072 exp 1.659 32173.515: Restored state 30 at -42.0 for tiltInd 33 def 5.655 IS -0.545 -0.373 32173.515: SetIntensity called with factor 1.0057 32173.531: Changing (ideal) Record exposure by 1.0057 to 1.6686 32173.546: Frame time for constraint is 0.1598 32173.562: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.102 -0.055 um, 7.4 4.0 % Error in Z Prediction 0.067 Predicted X = 0.814 StdErr = 0.275 nFit = 4 nDrop = 0 Predicted Y = -0.026 StdErr = 0.042 nFit = 5 nDrop = 0 Predicted Z = 5.681 StdErr = 0.522 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.169, -0.026 um; position 0.644, 0.001 Measured defocus = -0.29 microns changed by 0.19 to target Tracking image shifted 0.004, -0.002 um; position 0.641, 0.003 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0033_-44.0.tif Frame alignment results: distance raw = 10.2 smoothed = 10.2 Weighted resid mean = 0.92 max max = 1.62 Mean unweighted max = 2.23 Record image shifted 0.009, -0.012 um Tracking reference shifted by -4.8,10.3 pixels, 0.009,-0.012 um Saved Z = 33 at 16.51 min Tilt = -43.99 Mean = 38 Exp = 1.669 X/Y/Z = 0.632 0.015 5.871 32207.343: Saved state 33 at -44.0: def 5.771 IS -0.546 -0.327 int 0.12072 exp 1.669 32207.359: SetIntensity called with factor 1.0062 32207.375: Changing (ideal) Record exposure by 1.0062 to 1.6789 32207.390: Frame time for constraint is 0.1598 32207.406: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.182 0.041 um, 13.2 3.0 % Error in Z Prediction 0.189 Predicted X = 0.654 StdErr = 0.050 nFit = 3 nDrop = 0 Predicted Y = 0.078 StdErr = 0.043 nFit = 5 nDrop = 0 Predicted Z = 5.904 StdErr = 0.436 nFit = 6 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.029, 0.074 um; position 0.626, 0.004 Measured defocus = -0.19 microns changed by 0.09 to target Tracking image shifted 0.003, -0.002 um; position 0.622, 0.006 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0034_-46.0.tif Frame alignment results: distance raw = 14.0 smoothed = 13.8 Weighted resid mean = 1.16 max max = 1.90 Mean unweighted max = 3.53 Record image shifted 0.002, -0.020 um Tracking reference shifted by 1.5,14.8 pixels, 0.002,-0.020 um Saved Z = 34 at 17.00 min Tilt = -45.99 Mean = 37 Exp = 1.679 X/Y/Z = 0.621 0.026 5.992 32236.828: Saved state 34 at -46.0: def 5.892 IS -0.541 -0.312 int 0.12072 exp 1.679 32236.906: Restored state 32 at 22.0 for tiltInd 35 def 2.215 IS -0.549 -0.196 32236.921: SetIntensity called with factor 1.0057 32236.937: Changing (ideal) Record exposure by 1.0057 to 1.6682 32236.953: Frame time for constraint is 0.1598 32236.953: Will be constrained to 1.5979 (frame 0.1598, constraint 0.1598) 32244.750: recentlyReversed: dir 1 ang 23.999560 revtilt 26.993693 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.034 -0.052 um, 2.5 3.8 % Error in Z Prediction 0.088 Predicted X = 0.680 StdErr = 0.121 nFit = 3 nDrop = 0 Predicted Y = 0.069 StdErr = 0.036 nFit = 5 nDrop = 0 Predicted Z = 2.185 StdErr = 0.094 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.128, -0.029 um; position 0.552, 0.098 Measured defocus = -0.04 microns changed by -0.06 to target Tracking image shifted 0.002, -0.003 um; position 0.550, 0.101 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0035_24.0.tif Frame alignment results: distance raw = 24.1 smoothed = 23.5 Weighted resid mean = 0.12 max max = 0.21 Mean unweighted max = 0.58 Record image shifted -0.003, 0.002 um Tracking reference shifted by 2.3,-1.6 pixels, -0.003,0.002 um Saved Z = 35 at 17.59 min Tilt = 24.00 Mean = 44 Exp = 1.668 X/Y/Z = 0.553 0.099 2.124 32272.375: Saved state 35 at 24.0: def 2.024 IS -0.519 -0.210 int 0.12072 exp 1.668 32272.390: SetIntensity called with factor 1.0061 32272.406: Changing (ideal) Record exposure by 1.0061 to 1.6784 32272.421: Frame time for constraint is 0.1598 32272.421: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32275.625: recentlyReversed: dir 1 ang 25.999315 revtilt 28.997947 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.127 0.030 um, 9.2 2.2 % Error in Z Prediction -0.061 Predicted X = 0.545 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.018 StdErr = 0.041 nFit = 5 nDrop = 0 Predicted Z = 2.039 StdErr = 0.082 nFit = 6 nDrop = 0 Need regular tracking - Tracking image shifted -0.012, -0.036 um; position 0.557, 0.054 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0036_26.0.tif Frame alignment results: distance raw = 20.3 smoothed = 19.8 Weighted resid mean = 0.13 max max = 0.20 Mean unweighted max = 0.34 Record image shifted 0.000, -0.005 um Tracking reference shifted by 0.6,3.4 pixels, 0.000,-0.005 um Saved Z = 36 at 18.02 min Tilt = 26.00 Mean = 43 Exp = 1.678 X/Y/Z = 0.557 0.059 2.039 32298.328: Saved state 36 at 26.0: def 1.939 IS -0.503 -0.249 int 0.12072 exp 1.678 32298.406: Restored state 34 at -46.0 for tiltInd 37 def 5.892 IS -0.541 -0.312 32298.421: SetIntensity called with factor 1.0066 32298.421: Changing (ideal) Record exposure by 1.0066 to 1.6900 32298.437: Frame time for constraint is 0.1598 32298.453: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.012 0.040 um, 0.9 2.9 % Predicted X = 0.562 StdErr = 0.051 nFit = 4 nDrop = 0 Predicted Y = 0.096 StdErr = 0.040 nFit = 6 nDrop = 0 Predicted Z = 6.136 StdErr = 0.032 nFit = 4 nDrop = 2 Need regular tracking - Tracking image shifted -0.021, 0.128 um; position 0.583, -0.032 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0037_-48.0.tif Frame alignment results: distance raw = 22.4 smoothed = 12.0 Weighted resid mean = 0.61 max max = 2.10 Mean unweighted max = 8.73 Record image shifted 0.000, -0.012 um Tracking reference shifted by 1.5,9.1 pixels, 0.000,-0.012 um Saved Z = 37 at 18.51 min Tilt = -47.99 Mean = 36 Exp = 1.690 X/Y/Z = 0.582 -0.020 6.136 32327.656: Saved state 37 at -48.0: def 6.036 IS -0.487 -0.331 int 0.12072 exp 1.690 32327.671: SetIntensity called with factor 1.0071 32327.687: Changing (ideal) Record exposure by 1.0071 to 1.7021 32327.703: Frame time for constraint is 0.1598 32327.734: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.020 -0.116 um, 1.5 8.4 % Predicted X = 0.545 StdErr = 0.025 nFit = 4 nDrop = 0 Predicted Y = -0.057 StdErr = 0.043 nFit = 5 nDrop = 1 Predicted Z = 6.271 StdErr = 0.039 nFit = 4 nDrop = 2 Need regular tracking - Need autofocus Tracking image shifted -0.044, 0.034 um; position 0.589, -0.091 Measured defocus = -0.07 microns changed by -0.03 to target Tracking image shifted 0.001, -0.000 um; position 0.588, -0.091 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0038_-50.0.tif Frame alignment results: distance raw = 16.7 smoothed = 15.5 Weighted resid mean = 1.93 max max = 3.54 Mean unweighted max = 7.28 Record image shifted 0.002, -0.021 um Tracking reference shifted by 1.4,15.4 pixels, 0.002,-0.021 um Saved Z = 38 at 18.99 min Tilt = -49.98 Mean = 34 Exp = 1.702 X/Y/Z = 0.586 -0.070 6.244 32356.609: Saved state 38 at -50.0: def 6.144 IS -0.467 -0.378 int 0.12072 exp 1.702 32356.718: Restored state 36 at 26.0 for tiltInd 39 def 1.939 IS -0.503 -0.249 32356.718: SetIntensity called with factor 1.0066 32356.734: Changing (ideal) Record exposure by 1.0066 to 1.6895 32356.750: Frame time for constraint is 0.1598 32356.765: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32364.859: recentlyReversed: dir 1 ang 27.999070 revtilt 30.983704 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.041 -0.013 um, 2.9 1.0 % Error in Z Prediction -0.027 Predicted X = 0.545 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.014 StdErr = 0.007 nFit = 5 nDrop = 0 Predicted Z = 1.925 StdErr = 0.092 nFit = 6 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.036, -0.033 um; position 0.509, 0.047 Measured defocus = -0.08 microns changed by -0.02 to target Tracking image shifted -0.000, -0.001 um; position 0.510, 0.048 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0039_28.0.tif Frame alignment results: distance raw = 30.8 smoothed = 30.2 Weighted resid mean = 0.16 max max = 0.37 Mean unweighted max = 30.57 Record image shifted -0.001, -0.001 um Tracking reference shifted by 1.3,0.8 pixels, -0.001,-0.001 um Saved Z = 39 at 19.60 min Tilt = 28.00 Mean = 43 Exp = 1.689 X/Y/Z = 0.511 0.050 1.908 32393.218: Saved state 39 at 28.0: def 1.808 IS -0.460 -0.231 int 0.12072 exp 1.689 32393.234: SetIntensity called with factor 1.0071 32393.250: Changing (ideal) Record exposure by 1.0071 to 1.7015 32393.265: Frame time for constraint is 0.1598 32393.265: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32396.437: recentlyReversed: dir 1 ang 29.999325 revtilt 32.997957 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.034 0.036 um, 2.5 2.6 % Error in Z Prediction -0.017 Predicted X = 0.499 StdErr = 0.038 nFit = 3 nDrop = 0 Predicted Y = 0.030 StdErr = 0.019 nFit = 5 nDrop = 0 Predicted Z = 1.768 StdErr = 0.085 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.018, 0.017 um; position 0.517, 0.013 Measured defocus = -0.17 microns changed by 0.07 to target Tracking image shifted 0.001, -0.003 um; position 0.516, 0.016 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0040_30.0.tif Frame alignment results: distance raw = 25.2 smoothed = 24.7 Weighted resid mean = 0.09 max max = 0.15 Mean unweighted max = 0.47 Record image shifted -0.000, -0.006 um Tracking reference shifted by 0.9,4.1 pixels, -0.000,-0.006 um Saved Z = 40 at 20.12 min Tilt = 30.00 Mean = 43 Exp = 1.701 X/Y/Z = 0.516 0.022 1.838 32424.421: Saved state 40 at 30.0: def 1.738 IS -0.450 -0.259 int 0.12072 exp 1.701 32424.515: Restored state 38 at -50.0 for tiltInd 41 def 6.144 IS -0.467 -0.378 32424.515: SetIntensity called with factor 1.0077 32424.531: Changing (ideal) Record exposure by 1.0077 to 1.7151 32424.546: Frame time for constraint is 0.1598 32424.562: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.017 -0.008 um, 1.2 0.6 % Error in Z Prediction 0.070 Predicted X = 0.579 StdErr = 0.045 nFit = 4 nDrop = 0 Predicted Y = -0.170 StdErr = 0.020 nFit = 5 nDrop = 1 Predicted Z = 6.364 StdErr = 0.007 nFit = 4 nDrop = 2 Need regular tracking - Need autofocus Tracking image shifted 0.041, -0.024 um; position 0.539, -0.146 Measured defocus = -0.09 microns changed by -0.01 to target Tracking image shifted 0.001, -0.002 um; position 0.537, -0.144 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0041_-52.0.tif Frame alignment results: distance raw = 46.9 smoothed = 25.6 Weighted resid mean = 1.70 max max = 4.00 Mean unweighted max = 20.84 Record image shifted -0.001, -0.013 um Tracking reference shifted by 2.5,9.1 pixels, -0.001,-0.013 um Saved Z = 41 at 20.70 min Tilt = -51.99 Mean = 32 Exp = 1.715 X/Y/Z = 0.538 -0.131 6.358 32459.296: Saved state 41 at -52.0: def 6.258 IS -0.397 -0.406 int 0.12072 exp 1.715 32459.312: SetIntensity called with factor 1.0082 32459.328: Changing (ideal) Record exposure by 1.0082 to 1.7292 32459.343: Frame time for constraint is 0.1598 32459.359: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.041 0.039 um, 3.0 2.8 % Error in Z Prediction -0.006 Predicted X = 0.509 StdErr = 0.061 nFit = 4 nDrop = 0 Predicted Y = -0.242 StdErr = 0.024 nFit = 6 nDrop = 0 Predicted Z = 6.482 StdErr = 0.006 nFit = 5 nDrop = 1 Need regular tracking - Tracking image shifted -0.035, -0.070 um; position 0.544, -0.172 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0042_-54.0.tif Frame alignment results: distance raw = 35.5 smoothed = 35.4 Weighted resid mean = 0.29 max max = 0.71 Mean unweighted max = 4.36 Record image shifted -0.011, 0.082 um Tracking reference shifted by -2.9,-61.1 pixels, -0.011,0.082 um Saved Z = 42 at 21.10 min Tilt = -53.99 Mean = 31 Exp = 1.729 X/Y/Z = 0.555 -0.254 6.482 32483.234: Saved state 42 at -54.0: def 6.382 IS -0.353 -0.524 int 0.12072 exp 1.729 32483.328: Restored state 40 at 30.0 for tiltInd 43 def 1.738 IS -0.450 -0.259 32483.328: SetIntensity called with factor 1.0076 32483.343: Changing (ideal) Record exposure by 1.0076 to 1.7144 32483.359: Frame time for constraint is 0.1598 32483.375: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32491.781: recentlyReversed: dir 1 ang 31.999080 revtilt 34.997712 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.046 -0.012 um, 3.3 0.9 % Predicted X = 0.495 StdErr = 0.067 nFit = 4 nDrop = 0 Predicted Y = 0.009 StdErr = 0.020 nFit = 5 nDrop = 0 Predicted Z = 1.716 StdErr = 0.063 nFit = 5 nDrop = 0 Need regular tracking - Tracking image shifted 0.060, -0.014 um; position 0.436, 0.023 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0043_32.0.tif Frame alignment results: distance raw = 28.3 smoothed = 28.2 Weighted resid mean = 0.16 max max = 0.44 Mean unweighted max = 28.25 Record image shifted -0.002, -0.002 um Tracking reference shifted by 2.0,1.0 pixels, -0.002,-0.002 um Saved Z = 43 at 21.63 min Tilt = 32.00 Mean = 42 Exp = 1.714 X/Y/Z = 0.438 0.025 1.716 32514.500: Saved state 43 at 32.0: def 1.616 IS -0.386 -0.214 int 0.12072 exp 1.714 32514.515: SetIntensity called with factor 1.0082 32514.531: Changing (ideal) Record exposure by 1.0082 to 1.7284 32514.546: Frame time for constraint is 0.1598 32514.562: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32517.796: recentlyReversed: dir 1 ang 33.999335 revtilt 36.997967 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.057 0.016 um, 4.1 1.2 % Predicted X = 0.415 StdErr = 0.061 nFit = 3 nDrop = 0 Predicted Y = 0.030 StdErr = 0.021 nFit = 5 nDrop = 0 Predicted Z = 1.609 StdErr = 0.070 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.029, 0.058 um; position 0.444, -0.029 Measured defocus = -0.09 microns changed by -0.01 to target Tracking image shifted 0.001, -0.002 um; position 0.443, -0.027 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0044_34.0.tif Frame alignment results: distance raw = 27.2 smoothed = 26.7 Weighted resid mean = 0.09 max max = 0.16 Mean unweighted max = 0.49 Record image shifted 0.000, -0.007 um Tracking reference shifted by 0.7,4.8 pixels, 0.000,-0.007 um Saved Z = 44 at 22.15 min Tilt = 34.00 Mean = 42 Exp = 1.728 X/Y/Z = 0.443 -0.020 1.596 32545.843: Saved state 44 at 34.0: def 1.496 IS -0.368 -0.257 int 0.12072 exp 1.728 32545.921: Restored state 42 at -54.0 for tiltInd 45 def 6.382 IS -0.353 -0.524 32545.937: SetIntensity called with factor 1.0088 32545.953: Changing (ideal) Record exposure by 1.0088 to 1.7445 32545.968: Frame time for constraint is 0.1598 32545.984: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.027 -0.050 um, 2.0 3.6 % Error in Z Prediction -0.013 Predicted X = 0.529 StdErr = 0.048 nFit = 3 nDrop = 0 Predicted Y = -0.374 StdErr = 0.031 nFit = 5 nDrop = 0 Predicted Z = 6.604 StdErr = 0.007 nFit = 5 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted 0.058, -0.067 um; position 0.471, -0.307 Measured defocus = 0.00 microns changed by -0.10 to target Tracking image shifted 0.001, -0.000 um; position 0.470, -0.307 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0045_-56.0.tif Frame alignment results: distance raw = 105.7 smoothed = 57.2 Weighted resid mean = 1.37 max max = 7.86 Mean unweighted max = 45.96 Record image shifted -0.016, 0.109 um Tracking reference shifted by -3.3,-81.1 pixels, -0.016,0.109 um Saved Z = 45 at 22.74 min Tilt = -55.99 Mean = 31 Exp = 1.745 X/Y/Z = 0.486 -0.416 6.502 32581.078: Saved state 45 at -56.0: def 6.402 IS -0.217 -0.629 int 0.12072 exp 1.745 32581.093: SetIntensity called with factor 1.0095 32581.109: Changing (ideal) Record exposure by 1.0095 to 1.7610 32581.125: Frame time for constraint is 0.1598 32581.140: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction -0.043 -0.042 um, 3.1 3.0 % Error in Z Prediction -0.102 Predicted X = 0.443 StdErr = 0.100 nFit = 4 nDrop = 0 Predicted Y = -0.613 StdErr = 0.021 nFit = 5 nDrop = 1 Predicted Z = 6.632 StdErr = 0.057 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.041, -0.208 um; position 0.483, -0.406 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0046_-58.0.tif Frame alignment results: distance raw = 45.7 smoothed = 45.3 Weighted resid mean = 0.23 max max = 0.56 Mean unweighted max = 4.11 Record image shifted -0.013, 0.088 um Tracking reference shifted by -2.8,-65.4 pixels, -0.013,0.088 um Saved Z = 46 at 23.14 min Tilt = -57.99 Mean = 30 Exp = 1.761 X/Y/Z = 0.496 -0.494 6.632 32605.078: Saved state 46 at -58.0: def 6.532 IS -0.189 -0.703 int 0.12072 exp 1.761 32605.187: Restored state 44 at 34.0 for tiltInd 47 def 1.496 IS -0.368 -0.257 32605.203: SetIntensity called with factor 1.0088 32605.218: Changing (ideal) Record exposure by 1.0088 to 1.7436 32605.234: Frame time for constraint is 0.1598 32605.250: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32613.984: recentlyReversed: dir 1 ang 35.999090 revtilt 38.980724 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.053 0.120 um, 3.8 8.7 % Predicted X = 0.393 StdErr = 0.061 nFit = 3 nDrop = 0 Predicted Y = -0.029 StdErr = 0.029 nFit = 6 nDrop = 0 Predicted Z = 1.502 StdErr = 0.029 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.010, 0.013 um; position 0.383, -0.042 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0047_36.0.tif Frame alignment results: distance raw = 34.8 smoothed = 34.6 Weighted resid mean = 0.18 max max = 0.38 Mean unweighted max = 100.19 Record image shifted -0.005, -0.008 um Tracking reference shifted by 5.0,4.9 pixels, -0.005,-0.008 um Saved Z = 47 at 23.66 min Tilt = 36.00 Mean = 41 Exp = 1.744 X/Y/Z = 0.388 -0.035 1.502 32636.546: Saved state 47 at 36.0: def 1.402 IS -0.315 -0.240 int 0.12072 exp 1.744 32636.562: SetIntensity called with factor 1.0094 32636.578: Changing (ideal) Record exposure by 1.0094 to 1.7600 32636.593: Frame time for constraint is 0.1598 32636.609: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32639.812: recentlyReversed: dir 1 ang 37.999345 revtilt 40.997977 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.004 -0.005 um, 0.3 0.4 % Predicted X = 0.373 StdErr = 0.044 nFit = 3 nDrop = 0 Predicted Y = -0.065 StdErr = 0.032 nFit = 5 nDrop = 0 Predicted Z = 1.397 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.037, 0.029 um; position 0.410, -0.094 Measured defocus = -0.10 microns changed by 0.00 to target Tracking image shifted -0.000, -0.001 um; position 0.410, -0.093 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0048_38.0.tif Frame alignment results: distance raw = 27.7 smoothed = 27.5 Weighted resid mean = 0.13 max max = 0.21 Mean unweighted max = 0.62 Record image shifted 0.002, -0.003 um Tracking reference shifted by -0.8,2.7 pixels, 0.002,-0.003 um Saved Z = 48 at 24.18 min Tilt = 38.00 Mean = 41 Exp = 1.760 X/Y/Z = 0.408 -0.090 1.397 32667.843: Saved state 48 at 38.0: def 1.297 IS -0.306 -0.299 int 0.12072 exp 1.760 32667.937: Restored state 46 at -58.0 for tiltInd 49 def 6.532 IS -0.189 -0.703 32667.953: SetIntensity called with factor 1.0102 32667.968: Changing (ideal) Record exposure by 1.0102 to 1.7789 32667.984: Frame time for constraint is 0.1598 32668.000: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.035 -0.024 um, 2.5 1.8 % Error in Z Prediction 0.000 Predicted X = 0.462 StdErr = 0.110 nFit = 4 nDrop = 0 Predicted Y = -0.674 StdErr = 0.062 nFit = 6 nDrop = 0 Predicted Z = 6.735 StdErr = 0.065 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.115 um; position 0.456, -0.559 Measured defocus = 0.20 microns changed by -0.30 to target Tracking image shifted -0.000, 0.004 um; position 0.456, -0.563 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0049_-60.0.tif Frame alignment results: distance raw = 71.5 smoothed = 44.5 Weighted resid mean = 1.16 max max = 3.76 Mean unweighted max = 26.93 Record image shifted -0.013, 0.125 um Tracking reference shifted by -8.0,-92.7 pixels, -0.013,0.125 um Saved Z = 49 at 24.77 min Tilt = -59.98 Mean = 29 Exp = 1.779 X/Y/Z = 0.469 -0.688 6.431 32703.390: Saved state 49 at -60.0: def 6.331 IS -0.073 -0.861 int 0.12072 exp 1.779 32703.406: SetIntensity called with factor 1.0109 32703.421: Changing (ideal) Record exposure by 1.0109 to 1.7984 32703.453: Frame time for constraint is 0.1598 32703.468: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.007 -0.014 um, 0.5 1.0 % Error in Z Prediction -0.304 Predicted X = 0.493 StdErr = 0.078 nFit = 4 nDrop = 0 Predicted Y = -0.832 StdErr = 0.077 nFit = 5 nDrop = 0 Predicted Z = 6.528 StdErr = 0.127 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.017, -0.073 um; position 0.476, -0.759 Measured defocus = -0.08 microns changed by -0.02 to target Tracking image shifted 0.001, -0.003 um; position 0.475, -0.755 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0050_-62.0.tif Frame alignment results: distance raw = 68.3 smoothed = 68.3 Weighted resid mean = 0.75 max max = 3.78 Mean unweighted max = 99.43 Record image shifted -0.009, 0.104 um Tracking reference shifted by -7.4,-76.9 pixels, -0.009,0.104 um Saved Z = 50 at 25.26 min Tilt = -61.98 Mean = 28 Exp = 1.798 X/Y/Z = 0.485 -0.859 6.517 32732.765: Saved state 50 at -62.0: def 6.417 IS -0.005 -1.021 int 0.12072 exp 1.798 32732.859: Restored state 48 at 38.0 for tiltInd 51 def 1.297 IS -0.306 -0.299 32732.875: SetIntensity called with factor 1.0101 32732.890: Changing (ideal) Record exposure by 1.0101 to 1.7778 32732.906: Frame time for constraint is 0.1598 32732.921: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32741.953: recentlyReversed: dir 1 ang 39.999100 revtilt 42.993733 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.008 -0.027 um, 0.6 2.0 % Error in Z Prediction -0.011 Predicted X = 0.378 StdErr = 0.055 nFit = 3 nDrop = 0 Predicted Y = -0.147 StdErr = 0.030 nFit = 5 nDrop = 0 Predicted Z = 1.291 StdErr = 0.028 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, -0.060 um; position 0.382, -0.087 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0051_40.0.tif Frame alignment results: distance raw = 30.3 smoothed = 30.0 Weighted resid mean = 0.20 max max = 0.48 Mean unweighted max = 30.15 Record image shifted -0.000, -0.007 um Tracking reference shifted by 1.0,4.8 pixels, -0.000,-0.007 um Saved Z = 51 at 25.79 min Tilt = 40.00 Mean = 40 Exp = 1.778 X/Y/Z = 0.382 -0.081 1.291 32764.515: Saved state 51 at 40.0: def 1.191 IS -0.287 -0.277 int 0.12072 exp 1.778 32764.531: SetIntensity called with factor 1.0108 32764.546: Changing (ideal) Record exposure by 1.0108 to 1.7970 32764.562: Frame time for constraint is 0.1598 32764.578: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32771.281: recentlyReversed: dir 1 ang 41.999355 revtilt 45.001487 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.003 0.066 um, 0.2 4.8 % Predicted X = 0.386 StdErr = 0.035 nFit = 3 nDrop = 0 Predicted Y = -0.091 StdErr = 0.039 nFit = 5 nDrop = 0 Predicted Z = 1.185 StdErr = 0.032 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, 0.019 um; position 0.391, -0.110 Measured defocus = 0.01 microns changed by -0.11 to target Tracking image shifted -0.000, -0.000 um; position 0.391, -0.110 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0052_42.0.tif Frame alignment results: distance raw = 32.3 smoothed = 32.4 Weighted resid mean = 0.19 max max = 0.42 Mean unweighted max = 99.43 Record image shifted -0.002, -0.009 um Tracking reference shifted by 2.9,5.9 pixels, -0.002,-0.009 um Saved Z = 52 at 26.37 min Tilt = 42.00 Mean = 39 Exp = 1.797 X/Y/Z = 0.394 -0.101 1.072 32799.125: Saved state 52 at 42.0: def 0.972 IS -0.288 -0.302 int 0.12072 exp 1.797 32799.203: Restored state 50 at -62.0 for tiltInd 53 def 6.417 IS -0.005 -1.021 32799.218: SetIntensity called with factor 1.0117 32799.234: Changing (ideal) Record exposure by 1.0117 to 1.8195 32799.250: Frame time for constraint is 0.1598 32799.265: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.007 -0.011 um, 0.5 0.8 % Error in Z Prediction -0.113 Predicted X = 0.483 StdErr = 0.053 nFit = 4 nDrop = 0 Predicted Y = -1.038 StdErr = 0.056 nFit = 6 nDrop = 0 Predicted Z = 6.527 StdErr = 0.089 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.015, -0.107 um; position 0.468, -0.931 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0053_-64.0.tif Frame alignment results: distance raw = 62.6 smoothed = 63.2 Weighted resid mean = 0.38 max max = 2.12 Mean unweighted max = 99.53 Trimming A by 31 31, reference by 26 26 Record image shifted -0.017, 0.131 um Tracking reference shifted by -5.9,-97.2 pixels, -0.017,0.131 um Saved Z = 53 at 26.88 min Tilt = -63.99 Mean = 28 Exp = 1.819 X/Y/Z = 0.484 -1.062 6.527 32829.671: Saved state 53 at -64.0: def 6.427 IS 0.091 -1.199 int 0.12072 exp 1.819 32829.687: SetIntensity called with factor 1.0126 32829.703: Changing (ideal) Record exposure by 1.0126 to 1.8425 32829.718: Frame time for constraint is 0.1598 32829.734: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.002 -0.024 um, 0.1 1.7 % Predicted X = 0.512 StdErr = 0.039 nFit = 4 nDrop = 0 Predicted Y = -1.281 StdErr = 0.053 nFit = 6 nDrop = 0 Predicted Z = 6.550 StdErr = 0.100 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.015, -0.140 um; position 0.497, -1.141 Measured defocus = -0.02 microns changed by -0.08 to target Tracking image shifted -0.000, -0.004 um; position 0.497, -1.137 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0054_-66.0.tif Frame alignment results: distance raw = 71.7 smoothed = 66.6 Weighted resid mean = 4.42 max max = 27.21 Mean unweighted max = 103.10 Trimming A by 36 41, reference by 36 36 Record image shifted -0.008, 0.131 um Tracking reference shifted by -11.8,-96.5 pixels, -0.008,0.131 um Saved Z = 54 at 27.37 min Tilt = -65.98 Mean = 28 Exp = 1.842 X/Y/Z = 0.506 -1.268 6.476 32859.312: Saved state 54 at -66.0: def 6.376 IS 0.171 -1.393 int 0.12072 exp 1.842 32859.406: Restored state 52 at 42.0 for tiltInd 55 def 0.972 IS -0.288 -0.302 32859.421: SetIntensity called with factor 1.0117 32859.453: Changing (ideal) Record exposure by 1.0117 to 1.8180 32859.468: Frame time for constraint is 0.1598 32859.484: Will be constrained to 1.7577 (frame 0.1598, constraint 0.1598) 32868.750: recentlyReversed: dir 1 ang 43.999110 revtilt 46.993743 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.006 0.013 um, 0.5 0.9 % Error in Z Prediction -0.075 Predicted X = 0.380 StdErr = 0.029 nFit = 3 nDrop = 0 Predicted Y = -0.102 StdErr = 0.040 nFit = 5 nDrop = 0 Predicted Z = 0.963 StdErr = 0.081 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, -0.009 um; position 0.380, -0.093 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0055_44.0.tif Frame alignment results: distance raw = 32.3 smoothed = 32.2 Weighted resid mean = 0.22 max max = 0.62 Mean unweighted max = 95.52 Record image shifted 0.001, -0.014 um Tracking reference shifted by 1.1,10.6 pixels, 0.001,-0.014 um Saved Z = 55 at 27.91 min Tilt = 44.00 Mean = 39 Exp = 1.818 X/Y/Z = 0.379 -0.078 0.963 32891.390: Saved state 55 at 44.0: def 0.863 IS -0.286 -0.273 int 0.12072 exp 1.818 32891.406: SetIntensity called with factor 1.0126 32891.421: Changing (ideal) Record exposure by 1.0126 to 1.8408 32891.437: Frame time for constraint is 0.1598 32891.453: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) 32894.671: recentlyReversed: dir 1 ang 45.999364 revtilt 48.986498 back 3.000000 3mte 0.300000 Errors in X/Y Prediction -0.001 0.024 um, 0.1 1.7 % Predicted X = 0.382 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = -0.043 StdErr = 0.035 nFit = 5 nDrop = 0 Predicted Z = 0.832 StdErr = 0.094 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, 0.062 um; position 0.389, -0.105 Measured defocus = -0.20 microns changed by 0.10 to target Tracking image shifted 0.001, -0.001 um; position 0.388, -0.104 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0056_46.0.tif Frame alignment results: distance raw = 36.6 smoothed = 36.8 Weighted resid mean = 0.17 max max = 0.29 Mean unweighted max = 94.49 Record image shifted -0.001, -0.011 um Tracking reference shifted by 2.2,7.9 pixels, -0.001,-0.011 um Saved Z = 56 at 28.43 min Tilt = 46.00 Mean = 38 Exp = 1.841 X/Y/Z = 0.389 -0.093 0.927 32923.062: Saved state 56 at 46.0: def 0.827 IS -0.288 -0.292 int 0.12072 exp 1.841 32923.156: Restored state 54 at -66.0 for tiltInd 57 def 6.376 IS 0.171 -1.393 32923.171: SetIntensity called with factor 1.0136 32923.187: Changing (ideal) Record exposure by 1.0136 to 1.8676 32923.203: Frame time for constraint is 0.1598 32923.234: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) Errors in X/Y Prediction 0.007 -0.050 um, 0.5 3.6 % Error in Z Prediction 0.096 Predicted X = 0.513 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = -1.479 StdErr = 0.022 nFit = 5 nDrop = 0 Predicted Z = 6.475 StdErr = 0.068 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.026, -0.170 um; position 0.486, -1.308 Measured defocus = -0.03 microns changed by -0.07 to target Tracking image shifted -0.002, 0.001 um; position 0.488, -1.310 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0057_-68.0.tif Frame alignment results: distance raw = 92.2 smoothed = 81.1 Weighted resid mean = 4.43 max max = 26.27 Mean unweighted max = 97.92 Trimming A by 46 51, reference by 41 46 Record image shifted -0.016, 0.150 um Tracking reference shifted by -8.8,-111.1 pixels, -0.016,0.150 um Saved Z = 57 at 29.04 min Tilt = -67.99 Mean = 27 Exp = 1.868 X/Y/Z = 0.504 -1.460 6.397 32959.671: Saved state 57 at -68.0: def 6.297 IS 0.263 -1.562 int 0.12072 exp 1.868 32959.687: SetIntensity called with factor 1.0147 32959.703: Changing (ideal) Record exposure by 1.0147 to 1.8951 32959.718: Frame time for constraint is 0.1598 32959.734: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) The stage is not at the expected tilt angle (at -69.89, expected -70.00, error 0.11). TILT SERIES CONTINUING WITH THIS ERROR MESSAGE: The stage is not at the expected tilt angle (at -69.89, expected -70.00, error 0.11). This is probably not due to a pole touch. Press: "Stop && Set Limit" to stop and set this as a limiting ('pole touch') angle, "Go On" to try to go on. Errors in X/Y Prediction -0.008 0.019 um, 0.6 1.4 % Error in Z Prediction -0.078 Predicted X = 0.514 StdErr = 0.029 nFit = 4 nDrop = 0 Predicted Y = -1.670 StdErr = 0.018 nFit = 6 nDrop = 0 Predicted Z = 6.423 StdErr = 0.086 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.006, -0.197 um; position 0.508, -1.473 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0058_-69.9.tif Frame alignment results: distance raw = 59.9 smoothed = 60.4 Weighted resid mean = 0.59 max max = 6.05 Mean unweighted max = 97.08 Trimming A by 51 56, reference by 51 56 Record image shifted -0.009, 0.120 um Tracking reference shifted by -9.6,-88.6 pixels, -0.009,0.120 um Saved Z = 58 at 29.54 min Tilt = -69.89 Mean = 27 Exp = 1.895 X/Y/Z = 0.517 -1.593 6.423 32989.218: Saved state 58 at -69.9: def 6.323 IS 0.315 -1.686 int 0.12072 exp 1.895 32989.312: Restored state 56 at 46.0 for tiltInd 59 def 0.827 IS -0.288 -0.292 32989.328: SetIntensity called with factor 1.0135 32989.343: Changing (ideal) Record exposure by 1.0135 to 1.8657 32989.359: Frame time for constraint is 0.1598 32989.375: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) 32998.890: recentlyReversed: dir 1 ang 47.999119 revtilt 50.997752 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.003 0.077 um, 0.2 5.6 % Predicted X = 0.383 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = -0.092 StdErr = 0.031 nFit = 5 nDrop = 0 Predicted Z = 0.780 StdErr = 0.115 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 66 110, reference by 20 20 Tracking image shifted -0.006, 0.020 um; position 0.389, -0.112 Measured defocus = -0.16 microns changed by 0.06 to target Trimming A by 65 115, reference by 20 20 Tracking image shifted 0.005, 0.026 um; position 0.384, -0.139 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009_0059_48.0.tif Frame alignment results: distance raw = 33.2 smoothed = 33.4 Weighted resid mean = 0.19 max max = 0.31 Mean unweighted max = 99.73 Record image shifted -0.001, -0.018 um Tracking reference shifted by 2.8,12.6 pixels, -0.001,-0.018 um Saved Z = 59 at 30.17 min Tilt = 48.00 Mean = 37 Exp = 1.866 X/Y/Z = 0.385 -0.121 0.866 33027.406: Saved state 59 at 48.0: def 0.766 IS -0.271 -0.314 int 0.12072 exp 1.866 33027.437: SetIntensity called with factor 1.0146 33027.453: Changing (ideal) Record exposure by 1.0146 to 1.8930 33027.468: Frame time for constraint is 0.1598 33027.484: Will be constrained to 1.9174 (frame 0.1598, constraint 0.1598) 33030.546: recentlyReversed: dir 1 ang 49.999374 revtilt 52.993508 back 3.000000 3mte 0.300000 Errors in X/Y Prediction 0.002 -0.029 um, 0.1 2.1 % Error in Z Prediction 0.086 Predicted X = 0.390 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -0.143 StdErr = 0.037 nFit = 5 nDrop = 0 Predicted Z = 0.726 StdErr = 0.091 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Redoing image because of insufficient counts Redoing image because of insufficient counts There have been 3 failures to obtain an image with sufficient counts (2 actual vs. 3 criterion unbinned counts per sec) THE SERIES IS CONSIDERED SUCCESSFULLY COMPLETED BASED ON TILT SERIES POLICY SETTINGS 2021/11/17 22:04 W:\Zhiyang\Krios\20211117\Y00000Lamella01\20211117_009.mrc 0 60 11520 8184 11313 -10.0 48.0 2.00 REG 30.2 1x1 Continuum_K3 26000 1 0.17 LD-DS 20.0 0.0 Tilt series acquired at item # 42 with label 69 (item at 329.55, -2.37, TS at 329.61, -0.84) ################# PostTomo Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully ################# PostTomo END Focus area changed from stored position for item, axis position 5.00 angle 0, subarea offset 0 0