18-Nov-21  17:07:16

Rough eucentricity: changing Z by -1.89 to -60.08, finished.
7836.562: (Initial) alignment to map 29 (43)
7836.562: Target X, Y: -357.141 185.333
7836.562: Map matches low dose View; staying in low dose but with exp 0.405 intensity 0.19459 bin 4
Trimming A by 85  122  105  130,  reference by 80  127  105  120
Disparity in stage position after aligning to center of map frame: 0.03 0.38
Aligning to target with image shift equivalent to stage shift: 0.03 0.38
7843.672: Map matches low dose Preview; staying in low dose but with exp 0.202 intensity 0.12073 bin 4
7855.687: Realigned to map item itself with image shift of 31.8 141.9 pixels
Disparity in stage position after aligning to map item itself: -0.02 0.60  (align shift -0.06 0.19)
Finished aligning to item in 2 rounds
Opened new file W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037.mrc

STARTING TILT SERIES WITH AUTOMATIC TERMINATION AFTER ERRORS.
7863.469: Skipping reverse tilt: dir -1 start 0.00 revang 3.00  revtilt 2.98 maxtilt 69.90  maxerr 0.10
Measured defocus = -8.43 microns   changed by 8.33 to target
Measured defocus = -0.17 microns   changed by 0.07 to target
7898.125: SetIntensity called with factor 0.9962
7898.125: Changing (ideal) Record exposure by 0.9962 to 1.5918
7898.125: Frame time for constraint is 0.1598
7898.125: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
7898.125: Skipping reverse tilt: dir -1 start -10.00 revang -7.00  revtilt 2.98 maxtilt 69.90  maxerr 0.10
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.001, -0.025 um;  position 0.071, -0.156
Measured defocus = -0.05 microns   changed by -0.05 to target
Tracking image shifted 0.001, -0.002 um;  position 0.070, -0.154
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0000_-10.0.tif
Frame alignment results:          distance raw = 14.1 smoothed = 12.8
 Weighted resid mean = 0.06  max max = 0.12  Mean unweighted max = 0.55
Saved Z = 0  at 0.00 min  Tilt = -10.00  Mean = 47  Exp = 1.592  X/Y/Z =   0.070   -0.154    3.453
7917.922: Saved state 0 at -10.0: def 3.353  IS 0.013 -0.174  int 0.12073  exp 1.592
7917.937: SetIntensity called with factor 1.0002
7917.937: Changing (ideal) Record exposure by 1.0002 to 1.5920
7917.937: Frame time for constraint is 0.1598
7917.937: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.008, 0.004 um;  position 0.079, -0.158
Measured defocus = -0.23 microns   changed by 0.13 to target
Tracking image shifted 0.005, -0.006 um;  position 0.074, -0.152
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0001_-12.0.tif
Frame alignment results:          distance raw = 11.4 smoothed = 10.4
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.37
Record image shifted 0.002, -0.011 um
Tracking reference shifted by 0.5,8.5 pixels, 0.002,-0.011 um
Saved Z = 1  at 0.47 min  Tilt = -11.99  Mean = 47  Exp = 1.592  X/Y/Z =   0.072   -0.141    3.584
7946.390: Saved state 1 at -12.0: def 3.484  IS 0.005 -0.163  int 0.12073  exp 1.592
7946.406: SetIntensity called with factor 1.0005
7946.406: Changing (ideal) Record exposure by 1.0005 to 1.5927
7946.406: Frame time for constraint is 0.1598
7946.406: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Predicted X =   0.074  StdErr =   0.000  nFit = 2  nDrop = 0
Predicted Y =  -0.127  StdErr =   0.000  nFit = 2  nDrop = 0
Predicted Z =   3.716  StdErr =   0.000  nFit = 2  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.003, -0.021 um;  position 0.072, -0.106
Measured defocus = -0.02 microns   changed by -0.08 to target
Tracking image shifted 0.004, -0.004 um;  position 0.068, -0.102
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0002_-14.0.tif
Frame alignment results:          distance raw = 11.0 smoothed = 10.1
 Weighted resid mean = 0.07  max max = 0.10  Mean unweighted max = 0.19
Record image shifted 0.001, -0.010 um
Tracking reference shifted by 0.3,7.5 pixels, 0.001,-0.010 um
Saved Z = 2  at 0.96 min  Tilt = -13.99  Mean = 47  Exp = 1.593  X/Y/Z =   0.066   -0.092    3.639
7975.453: Saved state 2 at -14.0: def 3.539  IS -0.013 -0.117  int 0.12073  exp 1.593
7975.531: Restored state 0 at -10.0 for tiltInd 3  def 3.353  IS 0.013 -0.174
7975.547: SetIntensity called with factor 1.0002
7975.547: Changing (ideal) Record exposure by 1.0002 to 1.5920
7975.547: Frame time for constraint is 0.1598
7975.547: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
7979.765: recentlyReversed: dir 1 ang -7.998020 revtilt -5.000387 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.008    0.035 um,   0.6   2.5 %   Error in Z Prediction   -0.077
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.063, -0.106 um;  position 0.007, -0.048
Measured defocus = -0.00 microns   changed by -0.10 to target
Tracking image shifted 0.004, -0.006 um;  position 0.003, -0.042
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0003_-8.0.tif
Frame alignment results:          distance raw = 12.2 smoothed = 11.2
 Weighted resid mean = 0.07  max max = 0.12  Mean unweighted max = 0.39
Record image shifted 0.000, 0.006 um
Tracking reference shifted by -1.1,-4.0 pixels, 0.000,0.006 um
Saved Z = 3  at 1.49 min  Tilt = -8.00  Mean = 47  Exp = 1.592  X/Y/Z =   0.003   -0.048    3.358
8007.265: Saved state 3 at -8.0: def 3.258  IS 0.020 -0.043  int 0.12073  exp 1.592
8007.265: SetIntensity called with factor 1.0005
8007.281: Changing (ideal) Record exposure by 1.0005 to 1.5927
8007.281: Frame time for constraint is 0.1598
8007.281: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8010.687: recentlyReversed: dir 1 ang -5.999265 revtilt -3.000632 back 3.000000 3mte 0.300000
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.003, 0.003 um;  position -0.000, -0.051
Measured defocus = -0.02 microns   changed by -0.08 to target
Tracking image shifted 0.003, -0.006 um;  position -0.003, -0.045
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0004_-6.0.tif
Frame alignment results:          distance raw = 12.3 smoothed = 11.3
 Weighted resid mean = 0.07  max max = 0.12  Mean unweighted max = 0.44
Record image shifted 0.000, 0.008 um
Tracking reference shifted by -1.2,-6.1 pixels, 0.000,0.008 um
Saved Z = 4  at 2.01 min  Tilt = -6.00  Mean = 47  Exp = 1.593  X/Y/Z =  -0.003   -0.054    3.275
8038.656: Saved state 4 at -6.0: def 3.175  IS 0.028 -0.046  int 0.12073  exp 1.593
8038.734: Restored state 2 at -14.0 for tiltInd 5  def 3.539  IS -0.013 -0.117
8038.734: SetIntensity called with factor 1.0008
8038.734: Changing (ideal) Record exposure by 1.0008 to 1.5940
8038.734: Frame time for constraint is 0.1598
8038.750: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Predicted X =   0.065  StdErr =   0.006  nFit = 3  nDrop = 0
Predicted Y =  -0.067  StdErr =   0.026  nFit = 3  nDrop = 0
Predicted Z =   3.745  StdErr =   0.057  nFit = 3  nDrop = 0
  Need autofocus
Measured defocus = -0.08 microns   changed by -0.02 to target
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0005_-16.0.tif
Frame alignment results:          distance raw = 9.9 smoothed = 9.2
 Weighted resid mean = 0.06  max max = 0.11  Mean unweighted max = 0.29
Record image shifted 0.062, -0.067 um
Saved Z = 5  at 2.48 min  Tilt = -16.00  Mean = 47  Exp = 1.594  X/Y/Z =   0.004   -0.000    3.726
8067.031: Saved state 5 at -16.0: def 3.626  IS -0.003 -0.002  int 0.12073  exp 1.594
8067.031: SetIntensity called with factor 1.0011
8067.047: Changing (ideal) Record exposure by 1.0011 to 1.5957
8067.047: Frame time for constraint is 0.1598
8067.047: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.062    0.067 um,   4.5   4.8 %   Error in Z Prediction   -0.019
Predicted X =  -0.021  StdErr =   0.042  nFit = 3  nDrop = 0
Predicted Y =   0.063  StdErr =   0.032  nFit = 3  nDrop = 0
Predicted Z =   3.791  StdErr =   0.024  nFit = 3  nDrop = 0
  Need regular tracking  -
Tracking image shifted -0.023, -0.016 um;  position 0.001, 0.079
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0006_-18.0.tif
Frame alignment results:          distance raw = 8.1 smoothed = 7.3
 Weighted resid mean = 0.08  max max = 0.13  Mean unweighted max = 0.18
Record image shifted 0.005, -0.018 um
Tracking reference shifted by -1.0,13.7 pixels, 0.005,-0.018 um
Saved Z = 6  at 2.87 min  Tilt = -17.99  Mean = 47  Exp = 1.596  X/Y/Z =  -0.004    0.097    3.791
8090.281: Saved state 6 at -18.0: def 3.691  IS -0.043 0.087  int 0.12073  exp 1.596
8090.359: Restored state 4 at -6.0 for tiltInd 7  def 3.175  IS 0.028 -0.046
8090.359: SetIntensity called with factor 1.0008
8090.375: Changing (ideal) Record exposure by 1.0008 to 1.5940
8090.375: Frame time for constraint is 0.1598
8090.375: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8095.125: recentlyReversed: dir 1 ang -3.998510 revtilt -1.005377 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.018    0.034 um,   1.3   2.5 %   
Predicted X =  -0.009  StdErr =   0.000  nFit = 2  nDrop = 0
Predicted Y =  -0.059  StdErr =   0.000  nFit = 2  nDrop = 0
Predicted Z =   3.192  StdErr =   0.000  nFit = 2  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.007, 0.019 um;  position -0.016, -0.078
Measured defocus = -0.03 microns   changed by -0.07 to target
Tracking image shifted 0.002, -0.006 um;  position -0.018, -0.072
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0007_-4.0.tif
Frame alignment results:          distance raw = 11.5 smoothed = 10.6
 Weighted resid mean = 0.06  max max = 0.10  Mean unweighted max = 0.23
Record image shifted -0.001, 0.008 um
Tracking reference shifted by -0.3,-5.9 pixels, -0.001,0.008 um
Saved Z = 7  at 3.42 min  Tilt = -4.00  Mean = 47  Exp = 1.594  X/Y/Z =  -0.017   -0.080    3.121
8122.969: Saved state 7 at -4.0: def 3.021  IS 0.053 -0.062  int 0.12073  exp 1.594
8122.984: SetIntensity called with factor 1.0011
8122.984: Changing (ideal) Record exposure by 1.0011 to 1.5957
8122.984: Frame time for constraint is 0.1598
8123.000: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8126.359: recentlyReversed: dir 1 ang -1.998755 revtilt 0.994378 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.008   -0.021 um,   0.6   1.5 %   Error in Z Prediction   -0.071
Predicted X =  -0.026  StdErr =   0.006  nFit = 3  nDrop = 0
Predicted Y =  -0.093  StdErr =   0.015  nFit = 3  nDrop = 0
Predicted Z =   3.015  StdErr =   0.053  nFit = 3  nDrop = 0
  Need regular tracking  -
Tracking image shifted -0.014, 0.060 um;  position -0.011, -0.153
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0008_-2.0.tif
Frame alignment results:          distance raw = 9.7 smoothed = 8.5
 Weighted resid mean = 0.06  max max = 0.09  Mean unweighted max = 0.21
Record image shifted 0.000, 0.005 um
Tracking reference shifted by -1.1,-3.9 pixels, 0.000,0.005 um
Saved Z = 8  at 3.84 min  Tilt = -2.00  Mean = 47  Exp = 1.596  X/Y/Z =  -0.012   -0.158    3.015
8148.656: Saved state 8 at -2.0: def 2.915  IS 0.085 -0.134  int 0.12073  exp 1.596
8148.734: Restored state 6 at -18.0 for tiltInd 9  def 3.691  IS -0.043 0.087
8148.734: SetIntensity called with factor 1.0014
8148.734: Changing (ideal) Record exposure by 1.0014 to 1.5979
8148.734: Frame time for constraint is 0.1598
8148.750: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.014   -0.065 um,   1.0   4.7 %   
Predicted X =  -0.048  StdErr =   0.041  nFit = 3  nDrop = 0
Predicted Y =   0.190  StdErr =   0.004  nFit = 3  nDrop = 0
Predicted Z =   3.862  StdErr =   0.031  nFit = 3  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.024, 0.038 um;  position -0.023, 0.152
Measured defocus = -0.05 microns   changed by -0.05 to target
Tracking image shifted 0.004, 0.000 um;  position -0.028, 0.151
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0009_-20.0.tif
Frame alignment results:          distance raw = 7.9 smoothed = 7.2
 Weighted resid mean = 0.08  max max = 0.11  Mean unweighted max = 0.33
Record image shifted -0.002, -0.005 um
Tracking reference shifted by 2.3,3.2 pixels, -0.002,-0.005 um
Saved Z = 9  at 4.36 min  Tilt = -19.99  Mean = 47  Exp = 1.598  X/Y/Z =  -0.025    0.156    3.808
8179.875: Saved state 9 at -20.0: def 3.708  IS -0.053 0.152  int 0.12073  exp 1.598
8179.890: SetIntensity called with factor 1.0017
8179.890: Changing (ideal) Record exposure by 1.0017 to 1.6006
8179.890: Frame time for constraint is 0.1598
8179.906: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.022   -0.034 um,   1.6   2.5 %   Error in Z Prediction   -0.054
Predicted X =  -0.038  StdErr =   0.011  nFit = 3  nDrop = 0
Predicted Y =   0.241  StdErr =   0.028  nFit = 3  nDrop = 0
Predicted Z =   3.868  StdErr =   0.041  nFit = 3  nDrop = 0
  Need regular tracking  -
Tracking image shifted -0.020, 0.087 um;  position -0.018, 0.154
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0010_-22.0.tif
Frame alignment results:          distance raw = 7.7 smoothed = 7.1
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.21
Record image shifted 0.003, -0.004 um
Tracking reference shifted by -1.7,3.6 pixels, 0.003,-0.004 um
Saved Z = 10  at 4.76 min  Tilt = -21.99  Mean = 47  Exp = 1.601  X/Y/Z =  -0.021    0.158    3.868
8203.359: Saved state 10 at -22.0: def 3.768  IS -0.057 0.151  int 0.12073  exp 1.601
8203.437: Restored state 8 at -2.0 for tiltInd 11  def 2.915  IS 0.085 -0.134
8203.437: SetIntensity called with factor 1.0014
8203.437: Changing (ideal) Record exposure by 1.0014 to 1.5979
8203.453: Frame time for constraint is 0.1598
8203.453: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8208.844: recentlyReversed: dir 1 ang 0.001000 revtilt 2.993633 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.016   -0.083 um,   1.2   6.0 %   
Predicted X =  -0.019  StdErr =   0.014  nFit = 3  nDrop = 0
Predicted Y =  -0.202  StdErr =   0.038  nFit = 3  nDrop = 0
Predicted Z =   2.897  StdErr =   0.070  nFit = 3  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.007, -0.007 um;  position -0.026, -0.195
Measured defocus = -0.29 microns   changed by 0.19 to target
Tracking image shifted 0.002, -0.009 um;  position -0.029, -0.186
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0011_0.0.tif
Frame alignment results:          distance raw = 13.2 smoothed = 12.1
 Weighted resid mean = 0.06  max max = 0.11  Mean unweighted max = 0.37
Record image shifted -0.004, 0.016 um
Tracking reference shifted by 0.9,-12.1 pixels, -0.004,0.016 um
Saved Z = 11  at 5.31 min  Tilt = 0.00  Mean = 47  Exp = 1.598  X/Y/Z =  -0.025   -0.202    3.090
8236.594: Saved state 11 at 0.0: def 2.990  IS 0.117 -0.165  int 0.12073  exp 1.598
8236.609: SetIntensity called with factor 1.0017
8236.609: Changing (ideal) Record exposure by 1.0017 to 1.6006
8236.609: Frame time for constraint is 0.1598
8236.625: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8240.015: recentlyReversed: dir 1 ang 1.999255 revtilt 4.993888 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.005    0.000 um,   0.4   0.0 %   Error in Z Prediction    0.194
Predicted X =  -0.025  StdErr =   0.013  nFit = 3  nDrop = 0
Predicted Y =  -0.269  StdErr =   0.026  nFit = 3  nDrop = 0
Predicted Z =   3.015  StdErr =   0.125  nFit = 3  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.003, -0.054 um;  position -0.023, -0.215
Measured defocus = 0.01 microns   changed by -0.11 to target
Tracking image shifted 0.003, -0.008 um;  position -0.026, -0.207
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0012_2.0.tif
Frame alignment results:          distance raw = 13.4 smoothed = 12.3
 Weighted resid mean = 0.06  max max = 0.09  Mean unweighted max = 0.28
Record image shifted -0.002, 0.010 um
Tracking reference shifted by -0.2,-7.2 pixels, -0.002,0.010 um
Saved Z = 12  at 5.83 min  Tilt = 2.00  Mean = 46  Exp = 1.601  X/Y/Z =  -0.024   -0.217    2.903
8267.531: Saved state 12 at 2.0: def 2.803  IS 0.123 -0.178  int 0.12073  exp 1.601
8267.609: Restored state 10 at -22.0 for tiltInd 13  def 3.768  IS -0.057 0.151
8267.609: SetIntensity called with factor 1.0020
8267.625: Changing (ideal) Record exposure by 1.0020 to 1.6039
8267.625: Frame time for constraint is 0.1598
8267.640: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.001    0.052 um,   0.1   3.8 %   Error in Z Prediction   -0.113
Predicted X =  -0.021  StdErr =   0.027  nFit = 4  nDrop = 0
Predicted Y =   0.117  StdErr =   0.014  nFit = 4  nDrop = 0
Predicted Z =   3.922  StdErr =   0.050  nFit = 3  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.013, -0.047 um;  position -0.034, 0.164
Measured defocus = -0.10 microns   changed by -0.00 to target
Tracking image shifted 0.003, 0.005 um;  position -0.038, 0.159
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0013_-24.0.tif
Frame alignment results:          distance raw = 9.5 smoothed = 8.9
 Weighted resid mean = 0.06  max max = 0.10  Mean unweighted max = 0.27
Record image shifted -0.002, -0.009 um
Tracking reference shifted by 2.7,6.5 pixels, -0.002,-0.009 um
Saved Z = 13  at 6.35 min  Tilt = -23.99  Mean = 47  Exp = 1.604  X/Y/Z =  -0.036    0.168    3.916
8298.953: Saved state 13 at -24.0: def 3.816  IS -0.049 0.168  int 0.12073  exp 1.604
8298.953: SetIntensity called with factor 1.0024
8298.969: Changing (ideal) Record exposure by 1.0024 to 1.6076
8298.969: Frame time for constraint is 0.1598
8298.984: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.015    0.051 um,   1.1   3.7 %   Error in Z Prediction   -0.006
Predicted X =  -0.038  StdErr =   0.014  nFit = 3  nDrop = 0
Predicted Y =   0.120  StdErr =   0.030  nFit = 5  nDrop = 0
Predicted Z =   3.970  StdErr =   0.026  nFit = 4  nDrop = 0
  Need regular tracking  -
Tracking image shifted -0.014, -0.014 um;  position -0.024, 0.134
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0014_-26.0.tif
Frame alignment results:          distance raw = 9.8 smoothed = 8.7
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.38
Record image shifted 0.003, -0.001 um
Tracking reference shifted by -2.4,1.4 pixels, 0.003,-0.001 um
Saved Z = 14  at 6.74 min  Tilt = -25.99  Mean = 47  Exp = 1.608  X/Y/Z =  -0.027    0.135    3.970
8322.515: Saved state 14 at -26.0: def 3.870  IS -0.041 0.134  int 0.12073  exp 1.608
8322.594: Restored state 12 at 2.0 for tiltInd 15  def 2.803  IS 0.123 -0.178
8322.594: SetIntensity called with factor 1.0020
8322.594: Changing (ideal) Record exposure by 1.0020 to 1.6038
8322.609: Frame time for constraint is 0.1598
8322.609: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8328.531: recentlyReversed: dir 1 ang 3.999010 revtilt 6.998143 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.010    0.016 um,   0.7   1.1 %   
Predicted X =  -0.032  StdErr =   0.010  nFit = 3  nDrop = 0
Predicted Y =  -0.251  StdErr =   0.022  nFit = 3  nDrop = 0
Predicted Z =   2.887  StdErr =   0.145  nFit = 3  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.008, -0.040 um;  position -0.041, -0.211
Measured defocus = 0.05 microns   changed by -0.15 to target
Tracking image shifted 0.001, -0.005 um;  position -0.042, -0.206
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0015_4.0.tif
Frame alignment results:          distance raw = 14.7 smoothed = 13.7
 Weighted resid mean = 0.08  max max = 0.13  Mean unweighted max = 0.38
Record image shifted -0.001, 0.008 um
Tracking reference shifted by -0.6,-6.0 pixels, -0.001,0.008 um
Saved Z = 15  at 7.30 min  Tilt = 4.00  Mean = 46  Exp = 1.604  X/Y/Z =  -0.041   -0.215    2.733
8356.094: Saved state 15 at 4.0: def 2.633  IS 0.137 -0.168  int 0.12073  exp 1.604
8356.094: SetIntensity called with factor 1.0023
8356.109: Changing (ideal) Record exposure by 1.0023 to 1.6076
8356.109: Frame time for constraint is 0.1598
8356.125: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8359.953: recentlyReversed: dir 1 ang 5.998765 revtilt 8.999397 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.009    0.036 um,   0.6   2.6 %   Error in Z Prediction   -0.154
Predicted X =  -0.052  StdErr =   0.020  nFit = 4  nDrop = 0
Predicted Y =  -0.187  StdErr =   0.008  nFit = 4  nDrop = 0
Predicted Z =   2.699  StdErr =   0.137  nFit = 4  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.015, 0.042 um;  position -0.038, -0.229
Measured defocus = -0.11 microns   changed by 0.01 to target
Tracking image shifted 0.002, -0.005 um;  position -0.039, -0.223
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0016_6.0.tif
Frame alignment results:          distance raw = 13.0 smoothed = 11.9
 Weighted resid mean = 0.09  max max = 0.15  Mean unweighted max = 0.31
Record image shifted -0.001, 0.012 um
Tracking reference shifted by -0.5,-8.8 pixels, -0.001,0.012 um
Saved Z = 16  at 7.83 min  Tilt = 6.00  Mean = 46  Exp = 1.608  X/Y/Z =  -0.038   -0.235    2.711
8387.734: Saved state 16 at 6.0: def 2.611  IS 0.144 -0.188  int 0.12073  exp 1.608
8387.812: Restored state 14 at -26.0 for tiltInd 17  def 3.870  IS -0.041 0.134
8387.828: SetIntensity called with factor 1.0027
8387.828: Changing (ideal) Record exposure by 1.0027 to 1.6120
8387.828: Frame time for constraint is 0.1598
8387.844: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.015   -0.049 um,   1.1   3.5 %   Error in Z Prediction    0.012
Predicted X =  -0.028  StdErr =   0.028  nFit = 4  nDrop = 0
Predicted Y =   0.067  StdErr =   0.022  nFit = 6  nDrop = 0
Predicted Z =   4.023  StdErr =   0.029  nFit = 4  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.014, -0.090 um;  position -0.042, 0.157
Measured defocus = -0.21 microns   changed by 0.11 to target
Tracking image shifted 0.003, 0.006 um;  position -0.045, 0.151
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0017_-28.0.tif
Frame alignment results:          distance raw = 8.9 smoothed = 8.3
 Weighted resid mean = 0.06  max max = 0.11  Mean unweighted max = 0.33
Record image shifted -0.002, -0.008 um
Tracking reference shifted by 2.4,5.6 pixels, -0.002,-0.008 um
Saved Z = 17  at 8.37 min  Tilt = -27.99  Mean = 47  Exp = 1.612  X/Y/Z =  -0.043    0.159    4.126
8420.328: Saved state 17 at -28.0: def 4.026  IS -0.039 0.163  int 0.12073  exp 1.612
8420.344: SetIntensity called with factor 1.0030
8420.344: Changing (ideal) Record exposure by 1.0030 to 1.6168
8420.359: Frame time for constraint is 0.1598
8420.359: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.015    0.092 um,   1.1   6.6 %   Error in Z Prediction    0.103
Predicted X =  -0.048  StdErr =   0.031  nFit = 4  nDrop = 0
Predicted Y =   0.150  StdErr =   0.040  nFit = 5  nDrop = 0
Predicted Z =   4.156  StdErr =   0.067  nFit = 4  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.005, -0.085 um;  position -0.042, 0.235
Measured defocus = -0.02 microns   changed by -0.08 to target
Tracking image shifted 0.003, 0.007 um;  position -0.045, 0.228
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0018_-30.0.tif
Frame alignment results:          distance raw = 12.8 smoothed = 12.1
 Weighted resid mean = 0.06  max max = 0.11  Mean unweighted max = 0.31
Record image shifted 0.001, -0.006 um
Tracking reference shifted by 0.2,4.4 pixels, 0.001,-0.006 um
Saved Z = 18  at 8.86 min  Tilt = -29.99  Mean = 47  Exp = 1.617  X/Y/Z =  -0.045    0.234    4.073
8449.453: Saved state 18 at -30.0: def 3.973  IS -0.072 0.231  int 0.12073  exp 1.617
8449.515: Restored state 16 at 6.0 for tiltInd 19  def 2.611  IS 0.144 -0.188
8449.515: SetIntensity called with factor 1.0027
8449.531: Changing (ideal) Record exposure by 1.0027 to 1.6119
8449.531: Frame time for constraint is 0.1598
8449.547: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8456.422: recentlyReversed: dir 1 ang 7.999520 revtilt 10.998653 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.002    0.084 um,   0.2   6.0 %   Error in Z Prediction   -0.083
Predicted X =  -0.042  StdErr =   0.025  nFit = 4  nDrop = 0
Predicted Y =  -0.224  StdErr =   0.028  nFit = 5  nDrop = 0
Predicted Z =   2.612  StdErr =   0.106  nFit = 5  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.008, 0.034 um;  position -0.050, -0.258
Measured defocus = -0.03 microns   changed by -0.07 to target
Tracking image shifted 0.001, -0.001 um;  position -0.051, -0.257
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0019_8.0.tif
Frame alignment results:          distance raw = 18.2 smoothed = 17.1
 Weighted resid mean = 0.06  max max = 0.11  Mean unweighted max = 0.36
Record image shifted -0.002, 0.010 um
Tracking reference shifted by 0.1,-7.6 pixels, -0.002,0.010 um
Saved Z = 19  at 9.43 min  Tilt = 8.00  Mean = 46  Exp = 1.612  X/Y/Z =  -0.049   -0.267    2.542
8483.969: Saved state 19 at 8.0: def 2.442  IS 0.168 -0.209  int 0.12073  exp 1.612
8483.984: SetIntensity called with factor 1.0030
8483.984: Changing (ideal) Record exposure by 1.0030 to 1.6167
8484.000: Frame time for constraint is 0.1598
8484.000: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8487.390: recentlyReversed: dir 1 ang 9.999275 revtilt 12.978910 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.007   -0.043 um,   0.5   3.1 %   Error in Z Prediction   -0.070
Predicted X =  -0.051  StdErr =   0.011  nFit = 3  nDrop = 0
Predicted Y =  -0.299  StdErr =   0.018  nFit = 5  nDrop = 1
Predicted Z =   2.477  StdErr =   0.095  nFit = 6  nDrop = 0
  Need regular tracking  -
Tracking image shifted -0.012, 0.031 um;  position -0.039, -0.331
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0020_10.0.tif
Frame alignment results:          distance raw = 12.3 smoothed = 10.9
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.17
Record image shifted 0.001, 0.009 um
Tracking reference shifted by -1.7,-6.6 pixels, 0.001,0.009 um
Saved Z = 20  at 9.86 min  Tilt = 10.00  Mean = 46  Exp = 1.617  X/Y/Z =  -0.039   -0.340    2.477
8509.656: Saved state 20 at 10.0: def 2.377  IS 0.195 -0.279  int 0.12073  exp 1.617
8509.734: Restored state 18 at -30.0 for tiltInd 21  def 3.973  IS -0.072 0.231
8509.750: SetIntensity called with factor 1.0034
8509.750: Changing (ideal) Record exposure by 1.0034 to 1.6223
8509.765: Frame time for constraint is 0.1598
8509.765: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.011   -0.041 um,   0.8   2.9 %   
Predicted X =  -0.057  StdErr =   0.010  nFit = 3  nDrop = 0
Predicted Y =   0.274  StdErr =   0.050  nFit = 6  nDrop = 0
Predicted Z =   4.182  StdErr =   0.084  nFit = 4  nDrop = 0
  Need regular tracking  -
Tracking image shifted 0.004, -0.006 um;  position -0.060, 0.280
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0021_-32.0.tif
Frame alignment results:          distance raw = 8.6 smoothed = 7.8
 Weighted resid mean = 0.05  max max = 0.10  Mean unweighted max = 0.31
Record image shifted 0.002, 0.002 um
Tracking reference shifted by -1.9,-1.3 pixels, 0.002,0.002 um
Saved Z = 21  at 10.32 min  Tilt = -31.99  Mean = 47  Exp = 1.622  X/Y/Z =  -0.063    0.277    4.182
8537.297: Saved state 21 at -32.0: def 4.082  IS -0.078 0.279  int 0.12073  exp 1.622
8537.297: SetIntensity called with factor 1.0037
8537.312: Changing (ideal) Record exposure by 1.0037 to 1.6283
8537.312: Frame time for constraint is 0.1598
8537.328: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.006    0.003 um,   0.4   0.3 %   
Predicted X =  -0.070  StdErr =   0.011  nFit = 3  nDrop = 0
Predicted Y =   0.392  StdErr =   0.049  nFit = 5  nDrop = 0
Predicted Z =   4.244  StdErr =   0.095  nFit = 4  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.010, 0.106 um;  position -0.059, 0.286
Measured defocus = -0.13 microns   changed by 0.03 to target
Tracking image shifted 0.002, 0.005 um;  position -0.061, 0.281
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0022_-34.0.tif
Frame alignment results:          distance raw = 11.1 smoothed = 10.3
 Weighted resid mean = 0.08  max max = 0.14  Mean unweighted max = 0.21
Record image shifted -0.001, -0.006 um
Tracking reference shifted by 1.6,4.5 pixels, -0.001,-0.006 um
Saved Z = 22  at 10.81 min  Tilt = -33.99  Mean = 47  Exp = 1.628  X/Y/Z =  -0.060    0.287    4.266
8566.625: Saved state 22 at -34.0: def 4.166  IS -0.085 0.286  int 0.12073  exp 1.628
8566.719: Restored state 20 at 10.0 for tiltInd 23  def 2.377  IS 0.195 -0.279
8566.734: SetIntensity called with factor 1.0034
8566.734: Changing (ideal) Record exposure by 1.0034 to 1.6221
8566.750: Frame time for constraint is 0.1598
8566.750: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8573.937: recentlyReversed: dir 1 ang 11.999530 revtilt 14.995163 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.010   -0.104 um,   0.7   7.5 %   Error in Z Prediction    0.022
Predicted X =  -0.044  StdErr =   0.016  nFit = 3  nDrop = 0
Predicted Y =  -0.425  StdErr =   0.013  nFit = 5  nDrop = 1
Predicted Z =   2.376  StdErr =   0.104  nFit = 6  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.006, -0.039 um;  position -0.050, -0.386
Measured defocus = -0.09 microns   changed by -0.01 to target
Tracking image shifted -0.001, 0.001 um;  position -0.048, -0.387
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0023_12.0.tif
Frame alignment results:          distance raw = 16.7 smoothed = 15.8
 Weighted resid mean = 0.09  max max = 0.15  Mean unweighted max = 0.34
Record image shifted -0.003, 0.012 um
Tracking reference shifted by 0.4,-9.0 pixels, -0.003,0.012 um
Saved Z = 23  at 11.40 min  Tilt = 12.00  Mean = 45  Exp = 1.622  X/Y/Z =  -0.046   -0.399    2.370
8601.734: Saved state 23 at 12.0: def 2.270  IS 0.228 -0.328  int 0.12073  exp 1.622
8601.734: SetIntensity called with factor 1.0037
8601.750: Changing (ideal) Record exposure by 1.0037 to 1.6282
8601.765: Frame time for constraint is 0.1598
8601.765: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8605.062: recentlyReversed: dir 1 ang 13.999285 revtilt 16.998417 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.002    0.026 um,   0.2   1.9 %   Error in Z Prediction   -0.006
Predicted X =  -0.041  StdErr =   0.012  nFit = 3  nDrop = 0
Predicted Y =  -0.492  StdErr =   0.022  nFit = 5  nDrop = 0
Predicted Z =   2.222  StdErr =   0.072  nFit = 6  nDrop = 0
  Need regular tracking  -
Tracking image shifted -0.006, -0.047 um;  position -0.036, -0.445
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0024_14.0.tif
Frame alignment results:          distance raw = 10.5 smoothed = 9.6
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.35
Record image shifted -0.001, 0.011 um
Tracking reference shifted by -0.9,-7.9 pixels, -0.001,0.011 um
Saved Z = 24  at 11.82 min  Tilt = 14.00  Mean = 45  Exp = 1.628  X/Y/Z =  -0.035   -0.456    2.222
8627.390: Saved state 24 at 14.0: def 2.122  IS 0.246 -0.384  int 0.12073  exp 1.628
8627.484: Restored state 22 at -34.0 for tiltInd 25  def 4.166  IS -0.085 0.286
8627.484: SetIntensity called with factor 1.0041
8627.500: Changing (ideal) Record exposure by 1.0041 to 1.6350
8627.500: Frame time for constraint is 0.1598
8627.515: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.006    0.037 um,   0.5   2.6 %   
Predicted X =  -0.063  StdErr =   0.023  nFit = 4  nDrop = 0
Predicted Y =   0.316  StdErr =   0.048  nFit = 5  nDrop = 1
Predicted Z =   4.324  StdErr =   0.090  nFit = 4  nDrop = 0
  Need regular tracking  -
Tracking image shifted 0.010, 0.025 um;  position -0.073, 0.292
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0025_-36.0.tif
Frame alignment results:          distance raw = 6.8 smoothed = 6.0
 Weighted resid mean = 0.06  max max = 0.10  Mean unweighted max = 0.31
Record image shifted 0.002, 0.004 um
Tracking reference shifted by -1.8,-2.9 pixels, 0.002,0.004 um
Saved Z = 25  at 12.29 min  Tilt = -35.99  Mean = 47  Exp = 1.635  X/Y/Z =  -0.074    0.287    4.324
8655.234: Saved state 25 at -36.0: def 4.224  IS -0.073 0.294  int 0.12073  exp 1.635
8655.250: SetIntensity called with factor 1.0045
8655.250: Changing (ideal) Record exposure by 1.0045 to 1.6424
8655.265: Frame time for constraint is 0.1598
8655.265: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.011   -0.029 um,   0.8   2.1 %   
Predicted X =  -0.078  StdErr =   0.025  nFit = 4  nDrop = 0
Predicted Y =   0.251  StdErr =   0.012  nFit = 5  nDrop = 0
Predicted Z =   4.389  StdErr =   0.102  nFit = 4  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.011, -0.026 um;  position -0.067, 0.277
Measured defocus = -0.37 microns   changed by 0.27 to target
Tracking image shifted -0.000, -0.000 um;  position -0.067, 0.277
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0026_-38.0.tif
Frame alignment results:          distance raw = 7.4 smoothed = 6.9
 Weighted resid mean = 0.06  max max = 0.11  Mean unweighted max = 0.29
Record image shifted 0.001, -0.000 um
Tracking reference shifted by -0.4,0.3 pixels, 0.001,-0.000 um
Saved Z = 26  at 12.77 min  Tilt = -37.99  Mean = 47  Exp = 1.642  X/Y/Z =  -0.068    0.277    4.656
8684.234: Saved state 26 at -38.0: def 4.556  IS -0.074 0.281  int 0.12073  exp 1.642
8684.344: Restored state 24 at 14.0 for tiltInd 27  def 2.122  IS 0.246 -0.384
8684.344: SetIntensity called with factor 1.0041
8684.359: Changing (ideal) Record exposure by 1.0041 to 1.6348
8684.359: Frame time for constraint is 0.1598
8684.375: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8691.828: recentlyReversed: dir 1 ang 15.999040 revtilt 18.998672 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.011    0.027 um,   0.8   1.9 %   Error in Z Prediction    0.267
Predicted X =  -0.032  StdErr =   0.022  nFit = 4  nDrop = 0
Predicted Y =  -0.529  StdErr =   0.027  nFit = 5  nDrop = 0
Predicted Z =   2.106  StdErr =   0.079  nFit = 6  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.020, -0.088 um;  position -0.052, -0.441
Measured defocus = 0.11 microns   changed by -0.21 to target
Tracking image shifted -0.001, 0.001 um;  position -0.051, -0.442
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0027_16.0.tif
Frame alignment results:          distance raw = 12.0 smoothed = 11.2
 Weighted resid mean = 0.06  max max = 0.11  Mean unweighted max = 0.41
Record image shifted -0.003, 0.010 um
Tracking reference shifted by 1.1,-7.8 pixels, -0.003,0.010 um
Saved Z = 27  at 13.36 min  Tilt = 16.00  Mean = 45  Exp = 1.635  X/Y/Z =  -0.048   -0.452    1.893
8719.531: Saved state 27 at 16.0: def 1.793  IS 0.256 -0.374  int 0.12073  exp 1.635
8719.531: SetIntensity called with factor 1.0045
8719.547: Changing (ideal) Record exposure by 1.0045 to 1.6421
8719.562: Frame time for constraint is 0.1598
8719.578: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8722.875: recentlyReversed: dir 1 ang 17.999795 revtilt 20.994428 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.016    0.077 um,   1.1   5.5 %   Error in Z Prediction   -0.214
Predicted X =  -0.045  StdErr =   0.018  nFit = 3  nDrop = 0
Predicted Y =  -0.451  StdErr =   0.028  nFit = 5  nDrop = 0
Predicted Z =   1.795  StdErr =   0.134  nFit = 4  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.006, 0.004 um;  position -0.039, -0.455
Measured defocus = -0.10 microns   changed by 0.00 to target
Tracking image shifted -0.001, 0.000 um;  position -0.039, -0.456
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0028_18.0.tif
Frame alignment results:          distance raw = 5.0 smoothed = 4.0
 Weighted resid mean = 0.05  max max = 0.10  Mean unweighted max = 0.20
Record image shifted -0.000, 0.012 um
Tracking reference shifted by -1.5,-9.0 pixels, -0.000,0.012 um
Saved Z = 28  at 13.87 min  Tilt = 18.00  Mean = 44  Exp = 1.642  X/Y/Z =  -0.038   -0.468    1.797
8750.500: Saved state 28 at 18.0: def 1.697  IS 0.255 -0.393  int 0.12073  exp 1.642
8750.594: Restored state 26 at -38.0 for tiltInd 29  def 4.556  IS -0.074 0.281
8750.594: SetIntensity called with factor 1.0049
8750.609: Changing (ideal) Record exposure by 1.0049 to 1.6504
8750.625: Frame time for constraint is 0.1598
8750.625: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.007   -0.017 um,   0.5   1.2 %   Error in Z Prediction    0.002
Predicted X =  -0.068  StdErr =   0.026  nFit = 4  nDrop = 0
Predicted Y =   0.230  StdErr =   0.016  nFit = 6  nDrop = 0
Predicted Z =   4.694  StdErr =   0.177  nFit = 4  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.015, -0.092 um;  position -0.084, 0.322
Measured defocus = 0.17 microns   changed by -0.27 to target
Tracking image shifted 0.002, 0.013 um;  position -0.085, 0.310
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0029_-40.0.tif
Frame alignment results:          distance raw = 7.4 smoothed = 6.1
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.19
Record image shifted -0.002, -0.009 um
Tracking reference shifted by 2.5,6.1 pixels, -0.002,-0.009 um
Saved Z = 29  at 14.43 min  Tilt = -39.99  Mean = 47  Exp = 1.650  X/Y/Z =  -0.084    0.318    4.413
8784.015: Saved state 29 at -40.0: def 4.313  IS -0.080 0.326  int 0.12073  exp 1.650
8784.031: SetIntensity called with factor 1.0053
8784.031: Changing (ideal) Record exposure by 1.0053 to 1.6591
8784.047: Frame time for constraint is 0.1598
8784.047: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.015    0.088 um,   1.1   6.4 %   Error in Z Prediction   -0.281
Predicted X =  -0.089  StdErr =   0.029  nFit = 4  nDrop = 0
Predicted Y =   0.307  StdErr =   0.039  nFit = 6  nDrop = 0
Predicted Z =   4.649  StdErr =   0.239  nFit = 4  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.007, -0.069 um;  position -0.082, 0.375
Measured defocus = -0.08 microns   changed by -0.02 to target
Tracking image shifted 0.001, 0.006 um;  position -0.083, 0.370
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0030_-42.0.tif
Frame alignment results:          distance raw = 9.8 smoothed = 9.4
 Weighted resid mean = 0.06  max max = 0.10  Mean unweighted max = 0.22
Record image shifted 0.001, -0.003 um
Tracking reference shifted by -0.1,2.4 pixels, 0.001,-0.003 um
Saved Z = 30  at 14.92 min  Tilt = -41.99  Mean = 47  Exp = 1.659  X/Y/Z =  -0.084    0.373    4.625
8813.187: Saved state 30 at -42.0: def 4.525  IS -0.106 0.375  int 0.12073  exp 1.659
8813.265: Restored state 28 at 18.0 for tiltInd 31  def 1.697  IS 0.255 -0.393
8813.281: SetIntensity called with factor 1.0049
8813.297: Changing (ideal) Record exposure by 1.0049 to 1.6501
8813.312: Frame time for constraint is 0.1598
8813.312: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8821.156: recentlyReversed: dir 1 ang 19.998050 revtilt 22.995183 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.006    0.066 um,   0.4   4.8 %   Error in Z Prediction   -0.024
Predicted X =  -0.041  StdErr =   0.031  nFit = 4  nDrop = 0
Predicted Y =  -0.448  StdErr =   0.021  nFit = 6  nDrop = 0
Predicted Z =   1.631  StdErr =   0.124  nFit = 4  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.021, -0.025 um;  position -0.062, -0.423
Measured defocus = -0.27 microns   changed by 0.17 to target
Tracking image shifted -0.003, 0.006 um;  position -0.058, -0.429
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0031_20.0.tif
Frame alignment results:          distance raw = 8.7 smoothed = 8.2
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.22
Record image shifted -0.004, 0.013 um
Tracking reference shifted by 0.9,-9.7 pixels, -0.004,0.013 um
Saved Z = 31  at 15.52 min  Tilt = 20.00  Mean = 44  Exp = 1.650  X/Y/Z =  -0.055   -0.441    1.804
8849.078: Saved state 31 at 20.0: def 1.704  IS 0.256 -0.360  int 0.12073  exp 1.650
8849.078: SetIntensity called with factor 1.0053
8849.094: Changing (ideal) Record exposure by 1.0053 to 1.6587
8849.109: Frame time for constraint is 0.1598
8849.109: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8852.344: recentlyReversed: dir 1 ang 21.999305 revtilt 24.998937 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.014    0.006 um,   1.0   0.5 %   Error in Z Prediction    0.173
Predicted X =  -0.054  StdErr =   0.019  nFit = 3  nDrop = 0
Predicted Y =  -0.398  StdErr =   0.030  nFit = 5  nDrop = 0
Predicted Z =   1.575  StdErr =   0.135  nFit = 5  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.013, 0.099 um;  position -0.040, -0.497
Measured defocus = -0.44 microns   changed by 0.34 to target
Tracking image shifted -0.001, 0.002 um;  position -0.040, -0.500
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0032_22.0.tif
Frame alignment results:          distance raw = 4.1 smoothed = 3.2
 Weighted resid mean = 0.10  max max = 0.16  Mean unweighted max = 0.30
Record image shifted -0.002, 0.016 um
Tracking reference shifted by -0.7,-11.7 pixels, -0.002,0.016 um
Saved Z = 32  at 16.03 min  Tilt = 22.00  Mean = 43  Exp = 1.659  X/Y/Z =  -0.038   -0.515    1.916
8879.875: Saved state 32 at 22.0: def 1.816  IS 0.277 -0.435  int 0.12073  exp 1.659
8879.953: Restored state 30 at -42.0 for tiltInd 33  def 4.525  IS -0.106 0.375
8879.969: SetIntensity called with factor 1.0057
8879.984: Changing (ideal) Record exposure by 1.0057 to 1.6686
8879.984: Frame time for constraint is 0.1598
8880.000: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.016   -0.117 um,   1.2   8.5 %   Error in Z Prediction    0.341
Predicted X =  -0.097  StdErr =   0.026  nFit = 4  nDrop = 0
Predicted Y =   0.450  StdErr =   0.023  nFit = 5  nDrop = 1
Predicted Z =   4.726  StdErr =   0.185  nFit = 5  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.003, 0.010 um;  position -0.100, 0.440
Measured defocus = -0.31 microns   changed by 0.21 to target
Tracking image shifted 0.000, -0.001 um;  position -0.100, 0.440
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0033_-44.0.tif
Frame alignment results:          distance raw = 5.7 smoothed = 4.9
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.21
Record image shifted -0.000, 0.004 um
Tracking reference shifted by -0.6,-3.1 pixels, -0.000,0.004 um
Saved Z = 33  at 16.60 min  Tilt = -43.98  Mean = 47  Exp = 1.669  X/Y/Z =  -0.100    0.436    4.939
8913.828: Saved state 33 at -44.0: def 4.839  IS -0.122 0.439  int 0.12073  exp 1.669
8913.844: SetIntensity called with factor 1.0062
8913.859: Changing (ideal) Record exposure by 1.0062 to 1.6789
8913.875: Frame time for constraint is 0.1598
8913.875: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.003   -0.014 um,   0.2   1.0 %   Error in Z Prediction    0.213
Predicted X =  -0.108  StdErr =   0.021  nFit = 4  nDrop = 0
Predicted Y =   0.530  StdErr =   0.018  nFit = 6  nDrop = 0
Predicted Z =   4.932  StdErr =   0.230  nFit = 5  nDrop = 0
  Need autofocus
Measured defocus = 0.21 microns   changed by -0.31 to target
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0034_-46.0.tif
Frame alignment results:          distance raw = 16.2 smoothed = 15.0
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.34
Record image shifted -0.007, 0.071 um
Saved Z = 34  at 17.02 min  Tilt = -45.99  Mean = 47  Exp = 1.679  X/Y/Z =  -0.101    0.459    4.621
8939.515: Saved state 34 at -46.0: def 4.521  IS -0.132 0.460  int 0.12073  exp 1.679
8939.594: Restored state 32 at 22.0 for tiltInd 35  def 1.816  IS 0.277 -0.435
8939.609: SetIntensity called with factor 1.0057
8939.625: Changing (ideal) Record exposure by 1.0057 to 1.6682
8939.625: Frame time for constraint is 0.1598
8939.640: Will be constrained to 1.5979  (frame 0.1598, constraint 0.1598)
8947.422: recentlyReversed: dir 1 ang 23.999060 revtilt 26.994193 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.007   -0.071 um,   0.5   5.1 %   Error in Z Prediction   -0.311
Predicted X =  -0.043  StdErr =   0.025  nFit = 3  nDrop = 0
Predicted Y =  -0.509  StdErr =   0.062  nFit = 6  nDrop = 0
Predicted Z =   1.871  StdErr =   0.116  nFit = 4  nDrop = 1
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.009, 0.034 um;  position -0.052, -0.543
Measured defocus = -0.21 microns   changed by 0.11 to target
Tracking image shifted -0.004, 0.008 um;  position -0.048, -0.551
 10 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0035_24.0.tif
Frame alignment results:          distance raw = 6.7 smoothed = 6.1
 Weighted resid mean = 0.11  max max = 0.18  Mean unweighted max = 0.24
Record image shifted -0.004, 0.012 um
Tracking reference shifted by 1.1,-9.3 pixels, -0.004,0.012 um
Saved Z = 35  at 17.62 min  Tilt = 24.00  Mean = 43  Exp = 1.668  X/Y/Z =  -0.045   -0.563    1.985
8975.109: Saved state 35 at 24.0: def 1.885  IS 0.305 -0.473  int 0.12073  exp 1.668
8975.125: SetIntensity called with factor 1.0061
8975.140: Changing (ideal) Record exposure by 1.0061 to 1.6784
8975.156: Frame time for constraint is 0.1598
8975.156: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
8978.344: recentlyReversed: dir 1 ang 25.999315 revtilt 28.986449 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.002   -0.054 um,   0.1   3.9 %   Error in Z Prediction    0.114
Predicted X =  -0.033  StdErr =   0.038  nFit = 4  nDrop = 0
Predicted Y =  -0.651  StdErr =   0.055  nFit = 5  nDrop = 0
Predicted Z =   2.044  StdErr =   0.051  nFit = 4  nDrop = 2
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.002, -0.030 um;  position -0.031, -0.621
Measured defocus = -0.22 microns   changed by 0.12 to target
Tracking image shifted -0.002, 0.002 um;  position -0.029, -0.622
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0036_26.0.tif
Frame alignment results:          distance raw = 6.3 smoothed = 5.4
 Weighted resid mean = 0.09  max max = 0.14  Mean unweighted max = 0.31
Record image shifted -0.001, 0.017 um
Tracking reference shifted by -1.6,-12.6 pixels, -0.001,0.017 um
Saved Z = 36  at 18.14 min  Tilt = 26.00  Mean = 42  Exp = 1.678  X/Y/Z =  -0.028   -0.639    2.165
9006.640: Saved state 36 at 26.0: def 2.065  IS 0.328 -0.550  int 0.12073  exp 1.678
9006.719: Restored state 34 at -46.0 for tiltInd 37  def 4.521  IS -0.132 0.460
9006.734: SetIntensity called with factor 1.0066
9006.750: Changing (ideal) Record exposure by 1.0066 to 1.6901
9006.765: Frame time for constraint is 0.1598
9006.781: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.004    0.012 um,   0.3   0.8 %   Error in Z Prediction    0.121
Predicted X =  -0.110  StdErr =   0.021  nFit = 4  nDrop = 0
Predicted Y =   0.503  StdErr =   0.030  nFit = 5  nDrop = 0
Predicted Z =   4.860  StdErr =   0.243  nFit = 6  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.013, 0.052 um;  position -0.124, 0.451
Measured defocus = -0.09 microns   changed by -0.01 to target
Tracking image shifted 0.000, 0.007 um;  position -0.124, 0.444
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0037_-48.0.tif
Frame alignment results:          distance raw = 7.1 smoothed = 5.9
 Weighted resid mean = 0.07  max max = 0.11  Mean unweighted max = 0.30
Record image shifted -0.002, -0.003 um
Tracking reference shifted by 2.0,2.2 pixels, -0.002,-0.003 um
Saved Z = 37  at 18.73 min  Tilt = -47.99  Mean = 46  Exp = 1.690  X/Y/Z =  -0.122    0.448    4.837
9041.922: Saved state 37 at -48.0: def 4.737  IS -0.109 0.461  int 0.12073  exp 1.690
9041.937: SetIntensity called with factor 1.0071
9041.953: Changing (ideal) Record exposure by 1.0071 to 1.7021
9041.969: Frame time for constraint is 0.1598
9041.984: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.012   -0.056 um,   0.8   4.0 %   Error in Z Prediction   -0.022
Predicted X =  -0.136  StdErr =   0.023  nFit = 4  nDrop = 0
Predicted Y =   0.424  StdErr =   0.025  nFit = 5  nDrop = 1
Predicted Z =   4.866  StdErr =   0.238  nFit = 6  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.023, 0.016 um;  position -0.112, 0.408
Measured defocus = -0.22 microns   changed by 0.12 to target
Tracking image shifted -0.000, 0.008 um;  position -0.112, 0.400
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0038_-50.0.tif
Frame alignment results:          distance raw = 11.7 smoothed = 10.7
 Weighted resid mean = 0.09  max max = 0.15  Mean unweighted max = 0.33
Record image shifted 0.001, -0.003 um
Tracking reference shifted by -0.1,2.3 pixels, 0.001,-0.003 um
Saved Z = 38  at 19.22 min  Tilt = -49.98  Mean = 45  Exp = 1.702  X/Y/Z =  -0.113    0.403    4.981
9071.078: Saved state 38 at -50.0: def 4.881  IS -0.095 0.417  int 0.12073  exp 1.702
9071.156: Restored state 36 at 26.0 for tiltInd 39  def 2.065  IS 0.328 -0.550
9071.172: SetIntensity called with factor 1.0066
9071.187: Changing (ideal) Record exposure by 1.0066 to 1.6895
9071.203: Frame time for constraint is 0.1598
9071.219: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
9079.312: recentlyReversed: dir 1 ang 27.999070 revtilt 30.998202 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.023   -0.021 um,   1.6   1.5 %   Error in Z Prediction    0.115
Predicted X =  -0.027  StdErr =   0.018  nFit = 3  nDrop = 0
Predicted Y =  -0.755  StdErr =   0.053  nFit = 5  nDrop = 0
Predicted Z =   2.256  StdErr =   0.054  nFit = 4  nDrop = 2
  Need regular tracking  -
Tracking image shifted 0.037, -0.108 um;  position -0.064, -0.646
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0039_28.0.tif
Frame alignment results:          distance raw = 10.8 smoothed = 9.8
 Weighted resid mean = 0.11  max max = 0.19  Mean unweighted max = 0.32
Record image shifted -0.007, 0.028 um
Tracking reference shifted by 1.4,-21.0 pixels, -0.007,0.028 um
Saved Z = 39  at 19.73 min  Tilt = 28.00  Mean = 42  Exp = 1.689  X/Y/Z =  -0.057   -0.674    2.256
9102.031: Saved state 39 at 28.0: def 2.156  IS 0.368 -0.565  int 0.12073  exp 1.689
9102.047: SetIntensity called with factor 1.0071
9102.047: Changing (ideal) Record exposure by 1.0071 to 1.7015
9102.062: Frame time for constraint is 0.1598
9102.078: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
9105.265: recentlyReversed: dir 1 ang 29.999325 revtilt 32.997957 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.029    0.081 um,   2.1   5.8 %   
Predicted X =  -0.055  StdErr =   0.034  nFit = 3  nDrop = 0
Predicted Y =  -0.718  StdErr =   0.026  nFit = 5  nDrop = 1
Predicted Z =   2.371  StdErr =   0.066  nFit = 4  nDrop = 2
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.012, -0.003 um;  position -0.044, -0.715
Measured defocus = 0.25 microns   changed by -0.35 to target
Tracking image shifted -0.003, 0.010 um;  position -0.041, -0.725
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0040_30.0.tif
Frame alignment results:          distance raw = 5.7 smoothed = 4.1
 Weighted resid mean = 0.09  max max = 0.14  Mean unweighted max = 0.37
Record image shifted 0.000, 0.012 um
Tracking reference shifted by -1.7,-8.3 pixels, 0.000,0.012 um
Saved Z = 40  at 20.26 min  Tilt = 30.00  Mean = 41  Exp = 1.701  X/Y/Z =  -0.041   -0.736    2.028
9133.672: Saved state 40 at 30.0: def 1.928  IS 0.384 -0.628  int 0.12073  exp 1.701
9133.750: Restored state 38 at -50.0 for tiltInd 41  def 4.881  IS -0.095 0.417
9133.765: SetIntensity called with factor 1.0077
9133.781: Changing (ideal) Record exposure by 1.0077 to 1.7152
9133.797: Frame time for constraint is 0.1598
9133.797: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.014   -0.018 um,   1.0   1.3 %   Error in Z Prediction   -0.343
Predicted X =  -0.124  StdErr =   0.022  nFit = 3  nDrop = 0
Predicted Y =   0.320  StdErr =   0.004  nFit = 5  nDrop = 1
Predicted Z =   4.985  StdErr =   0.210  nFit = 7  nDrop = 0
  Need autofocus
Measured defocus = -0.48 microns   changed by 0.38 to target
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0041_-52.0.tif
Frame alignment results:          distance raw = 8.2 smoothed = 6.4
 Weighted resid mean = 0.09  max max = 0.14  Mean unweighted max = 0.51
Trimming A by 36  36,  reference by 21  26
Record image shifted 0.020, -0.063 um
Saved Z = 41  at 20.81 min  Tilt = -51.99  Mean = 44  Exp = 1.715  X/Y/Z =  -0.144    0.383    5.362
9166.969: Saved state 41 at -52.0: def 5.262  IS -0.059 0.416  int 0.12073  exp 1.715
9166.984: SetIntensity called with factor 1.0082
9167.000: Changing (ideal) Record exposure by 1.0082 to 1.7293
9167.031: Frame time for constraint is 0.1598
9167.031: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.020    0.063 um,   1.5   4.6 %   Error in Z Prediction    0.377
Predicted X =  -0.163  StdErr =   0.046  nFit = 4  nDrop = 0
Predicted Y =   0.297  StdErr =   0.032  nFit = 6  nDrop = 0
Predicted Z =   5.542  StdErr =   0.120  nFit = 4  nDrop = 3
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.034, -0.028 um;  position -0.129, 0.325
Measured defocus = -1.07 microns   changed by 0.97 to target
Tracking image shifted 0.013, -0.060 um;  position -0.142, 0.385
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0042_-54.0.tif
Frame alignment results:          distance raw = 9.6 smoothed = 8.6
 Weighted resid mean = 0.11  max max = 0.20  Mean unweighted max = 0.63
Trimming A by 23  56,  reference by 36  41
Record image shifted -0.001, 0.006 um
Tracking reference shifted by -0.1,-4.4 pixels, -0.001,0.006 um
Saved Z = 42  at 21.30 min  Tilt = -53.98  Mean = 44  Exp = 1.729  X/Y/Z =  -0.141    0.379    6.599
9196.359: Saved state 42 at -54.0: def 6.499  IS -0.060 0.411  int 0.12073  exp 1.729
9196.453: Restored state 40 at 30.0 for tiltInd 43  def 1.928  IS 0.384 -0.628
9196.469: SetIntensity called with factor 1.0076
9196.484: Changing (ideal) Record exposure by 1.0076 to 1.7144
9196.500: Frame time for constraint is 0.1598
9196.500: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
9204.922: recentlyReversed: dir 1 ang 31.999080 revtilt 34.999212 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.022    0.082 um,   1.6   5.9 %   Error in Z Prediction    1.057
Predicted X =  -0.055  StdErr =   0.033  nFit = 3  nDrop = 0
Predicted Y =  -0.785  StdErr =   0.028  nFit = 5  nDrop = 0
Predicted Z =   2.178  StdErr =   0.130  nFit = 6  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.013, -0.074 um;  position -0.068, -0.711
Measured defocus = 0.16 microns   changed by -0.26 to target
Tracking image shifted -0.004, 0.013 um;  position -0.064, -0.724
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0043_32.0.tif
Frame alignment results:          distance raw = 5.7 smoothed = 4.4
 Weighted resid mean = 0.13  max max = 0.20  Mean unweighted max = 0.30
Record image shifted -0.006, 0.017 um
Tracking reference shifted by 1.7,-13.4 pixels, -0.006,0.017 um
Saved Z = 43  at 21.92 min  Tilt = 32.00  Mean = 41  Exp = 1.714  X/Y/Z =  -0.058   -0.742    1.927
9233.047: Saved state 43 at 32.0: def 1.827  IS 0.402 -0.624  int 0.12073  exp 1.714
9233.062: SetIntensity called with factor 1.0082
9233.078: Changing (ideal) Record exposure by 1.0082 to 1.7285
9233.094: Frame time for constraint is 0.1598
9233.109: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
9236.281: recentlyReversed: dir 1 ang 33.999335 revtilt 36.997467 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.003    0.043 um,   0.2   3.1 %   Error in Z Prediction   -0.251
Predicted X =  -0.053  StdErr =   0.025  nFit = 3  nDrop = 0
Predicted Y =  -0.750  StdErr =   0.032  nFit = 5  nDrop = 0
Predicted Z =   2.007  StdErr =   0.163  nFit = 5  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.008, 0.020 um;  position -0.045, -0.770
Measured defocus = -0.13 microns   changed by 0.03 to target
Tracking image shifted -0.002, 0.005 um;  position -0.043, -0.776
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0044_34.0.tif
Frame alignment results:          distance raw = 7.3 smoothed = 6.6
 Weighted resid mean = 0.13  max max = 0.20  Mean unweighted max = 0.49
Record image shifted -0.001, 0.019 um
Tracking reference shifted by -1.5,-13.7 pixels, -0.001,0.019 um
Saved Z = 44  at 22.44 min  Tilt = 34.00  Mean = 40  Exp = 1.728  X/Y/Z =  -0.042   -0.794    2.040
9264.328: Saved state 44 at 34.0: def 1.940  IS 0.413 -0.679  int 0.12073  exp 1.728
9264.422: Restored state 42 at -54.0 for tiltInd 45  def 6.499  IS -0.060 0.411
9264.437: SetIntensity called with factor 1.0088
9264.453: Changing (ideal) Record exposure by 1.0088 to 1.7446
9264.453: Frame time for constraint is 0.1598
9264.469: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.011   -0.044 um,   0.8   3.2 %   Error in Z Prediction    0.033
Predicted X =  -0.160  StdErr =   0.049  nFit = 4  nDrop = 0
Predicted Y =   0.367  StdErr =   0.029  nFit = 5  nDrop = 0
Predicted Z =   6.156  StdErr =   0.601  nFit = 7  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.005, -0.063 um;  position -0.165, 0.430
Measured defocus = -0.51 microns   changed by 0.41 to target
Trimming A by 25  25,  reference by 20  20
Tracking image shifted 0.000, 0.015 um;  position -0.166, 0.415
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0045_-56.0.tif
Frame alignment results:          distance raw = 8.8 smoothed = 7.1
 Weighted resid mean = 0.15  max max = 0.24  Mean unweighted max = 0.66
Trimming A by 33  66,  reference by 46  51
Record image shifted -0.002, 0.004 um
Tracking reference shifted by 0.6,-3.2 pixels, -0.002,0.004 um
Saved Z = 45  at 23.03 min  Tilt = -55.99  Mean = 42  Exp = 1.745  X/Y/Z =  -0.164    0.411    6.542
9299.844: Saved state 45 at -56.0: def 6.442  IS -0.055 0.452  int 0.12073  exp 1.745
9299.859: SetIntensity called with factor 1.0095
9299.875: Changing (ideal) Record exposure by 1.0095 to 1.7611
9299.890: Frame time for constraint is 0.1598
9299.906: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.004    0.044 um,   0.3   3.2 %   Error in Z Prediction    0.387
Predicted X =  -0.168  StdErr =   0.039  nFit = 4  nDrop = 0
Predicted Y =   0.460  StdErr =   0.008  nFit = 5  nDrop = 1
Predicted Z =   7.016  StdErr =   0.464  nFit = 6  nDrop = 0
  Need regular tracking  -  Need autofocus
Trimming A by 20  30,  reference by 25  35
Tracking image shifted -0.006, 0.029 um;  position -0.163, 0.431
Measured defocus = -0.36 microns   changed by 0.26 to target
Trimming A by 22  35,  reference by 25  35
Tracking image shifted -0.001, -0.003 um;  position -0.162, 0.433
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0046_-58.0.tif
Frame alignment results:          distance raw = 7.5 smoothed = 6.4
 Weighted resid mean = 0.26  max max = 0.38  Mean unweighted max = 0.95
Trimming A by 33  76,  reference by 40  71
Record image shifted 0.001, 0.006 um
Tracking reference shifted by -1.6,-4.1 pixels, 0.001,0.006 um
Saved Z = 46  at 23.51 min  Tilt = -57.98  Mean = 41  Exp = 1.761  X/Y/Z =  -0.163    0.427    7.284
9328.969: Saved state 46 at -58.0: def 7.184  IS -0.064 0.466  int 0.12073  exp 1.761
9329.047: Restored state 44 at 34.0 for tiltInd 47  def 1.940  IS 0.413 -0.679
9329.062: SetIntensity called with factor 1.0088
9329.078: Changing (ideal) Record exposure by 1.0088 to 1.7436
9329.094: Frame time for constraint is 0.1598
9329.109: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
9337.672: recentlyReversed: dir 1 ang 35.999090 revtilt 38.998222 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.005   -0.033 um,   0.4   2.4 %   Error in Z Prediction    0.268
Predicted X =  -0.048  StdErr =   0.025  nFit = 3  nDrop = 0
Predicted Y =  -0.820  StdErr =   0.039  nFit = 5  nDrop = 0
Predicted Z =   1.986  StdErr =   0.133  nFit = 5  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.022, -0.052 um;  position -0.070, -0.768
Measured defocus = 0.01 microns   changed by -0.11 to target
Tracking image shifted -0.005, 0.012 um;  position -0.065, -0.780
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0047_36.0.tif
Frame alignment results:          distance raw = 9.0 smoothed = 8.5
 Weighted resid mean = 0.17  max max = 0.26  Mean unweighted max = 0.47
Record image shifted -0.006, 0.019 um
Tracking reference shifted by 1.7,-14.4 pixels, -0.006,0.019 um
Saved Z = 47  at 24.13 min  Tilt = 36.00  Mean = 39  Exp = 1.744  X/Y/Z =  -0.059   -0.798    1.886
9365.719: Saved state 47 at 36.0: def 1.786  IS 0.429 -0.673  int 0.12073  exp 1.744
9365.734: SetIntensity called with factor 1.0094
9365.750: Changing (ideal) Record exposure by 1.0094 to 1.7600
9365.765: Frame time for constraint is 0.1598
9365.781: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
9368.984: recentlyReversed: dir 1 ang 37.999345 revtilt 40.993978 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.011    0.022 um,   0.8   1.6 %   Error in Z Prediction   -0.100
Predicted X =  -0.054  StdErr =   0.025  nFit = 3  nDrop = 0
Predicted Y =  -0.806  StdErr =   0.040  nFit = 5  nDrop = 0
Predicted Z =   1.858  StdErr =   0.095  nFit = 5  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.009, 0.033 um;  position -0.045, -0.839
Measured defocus = -0.19 microns   changed by 0.09 to target
Tracking image shifted -0.002, 0.006 um;  position -0.043, -0.845
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0048_38.0.tif
Frame alignment results:          distance raw = 12.8 smoothed = 12.7
 Weighted resid mean = 0.17  max max = 0.28  Mean unweighted max = 0.94
Record image shifted -0.002, 0.020 um
Tracking reference shifted by -1.0,-14.8 pixels, -0.002,0.020 um
Saved Z = 48  at 24.65 min  Tilt = 38.00  Mean = 38  Exp = 1.760  X/Y/Z =  -0.040   -0.865    1.948
9396.969: Saved state 48 at 38.0: def 1.848  IS 0.445 -0.742  int 0.12073  exp 1.760
9397.047: Restored state 46 at -58.0 for tiltInd 49  def 7.184  IS -0.064 0.466
9397.062: SetIntensity called with factor 1.0102
9397.078: Changing (ideal) Record exposure by 1.0102 to 1.7790
9397.094: Frame time for constraint is 0.1598
9397.109: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.014   -0.059 um,   1.0   4.2 %   Error in Z Prediction    0.090
Predicted X =  -0.179  StdErr =   0.018  nFit = 3  nDrop = 0
Predicted Y =   0.486  StdErr =   0.017  nFit = 6  nDrop = 0
Predicted Z =   7.607  StdErr =   0.414  nFit = 7  nDrop = 0
  Need regular tracking  -  Need autofocus
Trimming A by 22  35,  reference by 30  40
Tracking image shifted 0.008, 0.011 um;  position -0.187, 0.474
Measured defocus = 0.12 microns   changed by -0.22 to target
Trimming A by 25  30,  reference by 30  40
Tracking image shifted -0.000, 0.008 um;  position -0.186, 0.466
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0049_-60.0.tif
Frame alignment results:          distance raw = 13.2 smoothed = 13.1
 Weighted resid mean = 0.27  max max = 0.46  Mean unweighted max = 1.26
Trimming A by 61  61,  reference by 34  81
Record image shifted -0.003, 0.017 um
Tracking reference shifted by -0.4,-12.6 pixels, -0.003,0.017 um
Saved Z = 49  at 25.25 min  Tilt = -59.99  Mean = 39  Exp = 1.779  X/Y/Z =  -0.184    0.449    7.371
9432.906: Saved state 49 at -60.0: def 7.271  IS -0.057 0.496  int 0.12073  exp 1.779
9432.922: SetIntensity called with factor 1.0109
9432.937: Changing (ideal) Record exposure by 1.0109 to 1.7984
9432.953: Frame time for constraint is 0.1598
9432.969: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction   -0.005   -0.036 um,   0.4   2.6 %   Error in Z Prediction   -0.236
Predicted X =  -0.190  StdErr =   0.016  nFit = 3  nDrop = 0
Predicted Y =   0.482  StdErr =   0.022  nFit = 5  nDrop = 0
Predicted Z =   8.052  StdErr =   0.389  nFit = 7  nDrop = 0
  Need regular tracking  -  Need autofocus
Trimming A by 20  20,  reference by 30  40
Tracking image shifted -0.015, 0.095 um;  position -0.175, 0.387
Measured defocus = 0.64 microns   changed by -0.74 to target
Trimming A by 25  30,  reference by 30  40
Tracking image shifted -0.000, -0.006 um;  position -0.174, 0.394
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0050_-62.0.tif
Frame alignment results:          distance raw = 10.9 smoothed = 9.3
 Weighted resid mean = 0.58  max max = 0.98  Mean unweighted max = 3.50
Trimming A by 23  76,  reference by 21  86
Record image shifted -0.002, 0.006 um
Tracking reference shifted by 0.7,-4.9 pixels, -0.002,0.006 um
Saved Z = 50  at 25.74 min  Tilt = -61.99  Mean = 36  Exp = 1.798  X/Y/Z =  -0.172    0.387    7.322
9462.578: Saved state 50 at -62.0: def 7.222  IS -0.037 0.435  int 0.12073  exp 1.798
9462.672: Restored state 48 at 38.0 for tiltInd 51  def 1.848  IS 0.445 -0.742
9462.687: SetIntensity called with factor 1.0101
9462.703: Changing (ideal) Record exposure by 1.0101 to 1.7778
9462.719: Frame time for constraint is 0.1598
9462.734: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
9472.172: recentlyReversed: dir 1 ang 39.999600 revtilt 42.998732 back 3.000000 3mte 0.300000
Errors in X/Y Prediction    0.017   -0.095 um,   1.3   6.9 %   Error in Z Prediction   -0.730
Predicted X =  -0.046  StdErr =   0.027  nFit = 3  nDrop = 0
Predicted Y =  -0.918  StdErr =   0.042  nFit = 5  nDrop = 0
Predicted Z =   1.905  StdErr =   0.097  nFit = 5  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.020, -0.077 um;  position -0.065, -0.841
Using peak at -6.6,0.8, rejecting 2.36 times higher peak at 0.0,-0.1
Measured defocus = -0.10 microns   changed by -0.00 to target
Tracking image shifted -0.004, 0.008 um;  position -0.061, -0.849
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0051_40.0.tif
Frame alignment results:          distance raw = 14.4 smoothed = 14.3
 Weighted resid mean = 0.19  max max = 0.33  Mean unweighted max = 0.86
Record image shifted -0.006, 0.016 um
Tracking reference shifted by 2.1,-12.2 pixels, -0.006,0.016 um
Saved Z = 51  at 26.37 min  Tilt = 40.00  Mean = 37  Exp = 1.778  X/Y/Z =  -0.055   -0.865    1.909
9500.219: Saved state 51 at 40.0: def 1.809  IS 0.458 -0.734  int 0.12073  exp 1.778
9500.234: SetIntensity called with factor 1.0108
9500.250: Changing (ideal) Record exposure by 1.0108 to 1.7971
9500.265: Frame time for constraint is 0.1598
9500.281: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
9503.515: recentlyReversed: dir 1 ang 41.999355 revtilt 44.998487 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.010    0.053 um,   0.7   3.8 %   Error in Z Prediction    0.004
Predicted X =  -0.048  StdErr =   0.025  nFit = 3  nDrop = 0
Predicted Y =  -0.887  StdErr =   0.049  nFit = 5  nDrop = 0
Predicted Z =   1.888  StdErr =   0.079  nFit = 6  nDrop = 0
  Need regular tracking  -
Tracking image shifted -0.005, 0.012 um;  position -0.043, -0.898
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0052_42.0.tif
Frame alignment results:          distance raw = 7.6 smoothed = 7.0
 Weighted resid mean = 0.18  max max = 0.31  Mean unweighted max = 0.81
Record image shifted -0.004, 0.029 um
Tracking reference shifted by -0.7,-21.5 pixels, -0.004,0.029 um
Saved Z = 52  at 26.81 min  Tilt = 42.00  Mean = 36  Exp = 1.797  X/Y/Z =  -0.038   -0.927    1.888
9526.375: Saved state 52 at 42.0: def 1.788  IS 0.473 -0.799  int 0.12073  exp 1.797
9526.453: Restored state 50 at -62.0 for tiltInd 53  def 7.222  IS -0.037 0.435
9526.469: SetIntensity called with factor 1.0117
9526.484: Changing (ideal) Record exposure by 1.0117 to 1.8195
9526.500: Frame time for constraint is 0.1598
9526.531: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.009   -0.041 um,   0.7   2.9 %   
Predicted X =  -0.182  StdErr =   0.023  nFit = 3  nDrop = 0
Predicted Y =   0.336  StdErr =   0.042  nFit = 5  nDrop = 0
Predicted Z =   7.706  StdErr =   0.347  nFit = 5  nDrop = 1
  Need regular tracking  -  Need autofocus
Trimming A by 25  25,  reference by 27  40
Tracking image shifted -0.004, 0.001 um;  position -0.178, 0.335
Measured defocus = 0.52 microns   changed by -0.62 to target
Trimming A by 25  25,  reference by 27  40
Tracking image shifted -0.002, 0.005 um;  position -0.176, 0.330
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0053_-64.0.tif
Frame alignment results:          distance raw = 26.1 smoothed = 22.1
 Weighted resid mean = 1.11  max max = 3.50  Mean unweighted max = 16.20
Trimming A by 76  81,  reference by 31  31
Record image shifted -0.003, -0.014 um
Tracking reference shifted by 4.1,9.5 pixels, -0.003,-0.014 um
Saved Z = 53  at 27.41 min  Tilt = -63.99  Mean = 26  Exp = 1.820  X/Y/Z =  -0.173    0.344    7.078
9562.406: Saved state 53 at -64.0: def 6.978  IS -0.016 0.397  int 0.12073  exp 1.820
9562.422: SetIntensity called with factor 1.0126
9562.437: Changing (ideal) Record exposure by 1.0126 to 1.8425
9562.453: Frame time for constraint is 0.1598
9562.469: Will be constrained to 1.9174  (frame 0.1598, constraint 0.1598)
Errors in X/Y Prediction    0.009    0.008 um,   0.7   0.6 %   Error in Z Prediction   -0.628
Predicted X =  -0.153  StdErr =   0.029  nFit = 4  nDrop = 0
Predicted Y =   0.262  StdErr =   0.030  nFit = 6  nDrop = 0
Predicted Z =   7.097  StdErr =   0.185  nFit = 4  nDrop = 2
  Need regular tracking  -  Need autofocus
Trimming A by 20  20,  reference by 30  35
Tracking image shifted 0.003, 0.016 um;  position -0.156, 0.246
Measured defocus = -25.72 microns   changed by 16.00
Measured defocus = -32.99 microns
WARNING: Autofocus aborted after 2 iterations and focus returned to starting value (7.00)
  because of inconsistent behavior. Check focus calibration
  and/or scope alignment if images and specimen look OK
Autofocus has failed to behave consistently after several iterations

ONE SIDE OF THE SERIES IS BEING ENDED;
GOING ON TO FINISH THE OTHER SIDE BASED ON TILT SERIES POLICY SETTINGS
9580.531: Restored state 52 at 42.0 for tiltInd 55  def 1.788  IS 0.473 -0.799
9580.547: SetIntensity called with factor 1.0117
9580.562: Changing (ideal) Record exposure by 1.0117 to 1.8180
9580.578: Frame time for constraint is 0.1598
9580.594: Will be constrained to 1.7577  (frame 0.1598, constraint 0.1598)
9589.734: recentlyReversed: dir 1 ang 43.999110 revtilt 46.993743 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.020    0.082 um,   1.4   5.9 %   Error in Z Prediction   -0.019
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.001, -0.019 um;  position -0.037, -0.908
Measured defocus = 0.46 microns   changed by -0.56 to target
Tracking image shifted -0.010, 0.001 um;  position -0.027, -0.910
 11 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0054_44.0.tif
Frame alignment results:          distance raw = 29.8 smoothed = 30.1
 Weighted resid mean = 0.45  max max = 0.77  Mean unweighted max = 29.02
Record image shifted -0.012, 0.022 um
Tracking reference shifted by 5.8,-17.3 pixels, -0.012,0.022 um
Saved Z = 54  at 28.29 min  Tilt = 44.00  Mean = 34  Exp = 1.818  X/Y/Z =  -0.015   -0.931    1.328
9615.594: Saved state 54 at 44.0: def 1.228  IS 0.455 -0.814  int 0.12073  exp 1.818
9615.594: SetIntensity called with factor 1.0125
9615.625: Changing (ideal) Record exposure by 1.0125 to 1.8408
9615.640: Frame time for constraint is 0.1598
9615.640: Will be constrained to 1.9174  (frame 0.1598, constraint 0.1598)
9618.859: recentlyReversed: dir 1 ang 45.999364 revtilt 48.998497 back 3.000000 3mte 0.300000
  Need regular tracking  -  Need autofocus
Tracking image shifted -0.022, -0.002 um;  position 0.007, -0.929
Measured defocus = -0.13 microns   changed by 0.03 to target
Tracking image shifted -0.004, -0.000 um;  position 0.010, -0.928
 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0055_46.0.tif
Frame alignment results:          distance raw = 33.0 smoothed = 32.7
 Weighted resid mean = 0.59  max max = 1.13  Mean unweighted max = 94.27
Trimming A by 21  31,  reference by 21  21
Record image shifted -0.003, 0.028 um
Tracking reference shifted by -1.6,-21.0 pixels, -0.003,0.028 um
Saved Z = 55  at 28.82 min  Tilt = 46.00  Mean = 33  Exp = 1.841  X/Y/Z =   0.014   -0.956    1.362
9647.078: Saved state 55 at 46.0: def 1.262  IS 0.443 -0.852  int 0.12073  exp 1.841
9647.109: SetIntensity called with factor 1.0135
9647.125: Changing (ideal) Record exposure by 1.0135 to 1.8657
9647.140: Frame time for constraint is 0.1598
9647.156: Will be constrained to 1.9174  (frame 0.1598, constraint 0.1598)
9650.453: recentlyReversed: dir 1 ang 47.999619 revtilt 50.998252 back 3.000000 3mte 0.300000
Predicted X =   0.043  StdErr =   0.000  nFit = 2  nDrop = 0
Predicted Y =  -0.982  StdErr =   0.000  nFit = 2  nDrop = 0
Predicted Z =   1.395  StdErr =   0.000  nFit = 2  nDrop = 0
  Need regular tracking  -  Need autofocus
Tracking image shifted 0.007, -0.018 um;  position 0.036, -0.964
Measured defocus = -0.22 microns   changed by 0.12 to target
Tracking image shifted -0.002, 0.000 um;  position 0.037, -0.964
 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0056_48.0.tif
Frame alignment results:          distance raw = 32.3 smoothed = 31.9
 Weighted resid mean = 0.99  max max = 1.73  Mean unweighted max = 95.88
Trimming A by 28  41,  reference by 26  31
Record image shifted -0.003, 0.031 um
Tracking reference shifted by -2.1,-23.1 pixels, -0.003,0.031 um
Saved Z = 56  at 29.34 min  Tilt = 48.00  Mean = 31  Exp = 1.866  X/Y/Z =   0.040   -0.995    1.516
9678.750: Saved state 56 at 48.0: def 1.416  IS 0.438 -0.901  int 0.12073  exp 1.866
9678.765: SetIntensity called with factor 1.0146
9678.781: Changing (ideal) Record exposure by 1.0146 to 1.8930
9678.797: Frame time for constraint is 0.1598
9678.828: Will be constrained to 1.9174  (frame 0.1598, constraint 0.1598)
9682.375: recentlyReversed: dir 1 ang 49.999374 revtilt 53.000507 back 3.000000 3mte 0.300000
Errors in X/Y Prediction   -0.002   -0.013 um,   0.2   1.0 %   Error in Z Prediction    0.120
Predicted X =   0.068  StdErr =   0.002  nFit = 3  nDrop = 0
Predicted Y =  -1.025  StdErr =   0.010  nFit = 3  nDrop = 0
Predicted Z =   1.589  StdErr =   0.090  nFit = 3  nDrop = 0
  Need regular tracking  -
Tracking image shifted 0.012, -0.014 um;  position 0.057, -1.012
 12 frames were saved to W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037_0057_50.0.tif
Frame alignment results:          distance raw = 17.9 smoothed = 16.9
 Weighted resid mean = 0.74  max max = 1.54  Mean unweighted max = 7.50
Trimming A by 36  51,  reference by 28  41
Record image shifted -0.003, 0.035 um
Tracking reference shifted by -2.3,-26.2 pixels, -0.003,0.035 um
Saved Z = 57  at 29.78 min  Tilt = 50.00  Mean = 29  Exp = 1.893  X/Y/Z =   0.060   -1.047    1.589
2021/11/18 17:39  W:\Zhiyang\Krios\20211117\Y00000Lamella03\20211117_037.mrc  0  58  11520  8184  10936  -10.0  50.0  2.00  REG  29.8  1x1  Continuum_K3  26000  1  0.17  LD-DS  20.0  0.0
Tilt series acquired at item # 29 with label 42 (item at -357.14, 185.33, TS at -357.06, 186.59)
################# PostTomo
Started a long running operation: updating hardware dark reference
Call for updating hardware dark reference finished successfully
################# PostTomo END
Focus area changed from stored position for item, axis position 5.50 angle 0, subarea offset 0 0