Two datasets were collected at different dates and merged for data processing.
Between the two datasets, 5,159 movies were collected.
All frames were used during frame alignment.
Only particles from dataset 19may24k were used for Bayesian polishing and all subsequent steps, including final C1 reconstruction, which is the particles.star file included.

Sample: Mechanosensitive Ion Channel Flycatcher1 in GDN
Grid: UltrAuFoil 300 mesh R1.2/1.3
Cemera: Gatan K2
Camera mode: Counting
Acquisition method: beam-image shift
Acquisition pattern: 2 x 2 holes, 1 image in the center of each hole
Physical Pixel: 1.03 A/pix
Data format: Frames
Total exposure: 50 e/A2
Defocus: -0.7 to -2.2 um
Exposure time per frame:  0.25 s
Particles for C1 map: 130k
Particles file: run_data.star
Optics groups: per dataset
Final resolution: 2.8 A

>For dataset 19may24k
Dose per frame:  1.16 e/A2
Frames: 43
Movies: 2648
Gain reference: 19may24k_ref.mrc
>For dataset 19jul28d
Dose per frame:  1.19 e/A2
Frames: 42
Movies: 2512
Gain reference: 19jul28d_ref.mrc