The original displacements in the overlap zones have mean 8.88 maximum 12.61 pixels. After shifting pieces into register, the displacements have mean 1.55 maximum 1.55 pixels. Running script 6 at item 8 (194.82, -27.17) Trimming A by 75 80, reference by 80 85 Trimming A by 70 70, reference by 80 85 Trimming A by 50 55, reference by 80 85 Rough eucentricity: changing Z by 24.77 to 124.78, continuing... Trimming A by 35 35, reference by 35 35 Trimming A by 35 35, reference by 35 35 Rough eucentricity: changing Z by -0.46 to 124.32, finished. Z = 0: The original displacements in the overlap zones have mean 16.22 maximum 29.60 pixels. After shifting pieces into register, the displacements have mean 4.13 maximum 4.13 pixels. Running script 1 at item 10 (196.15, -23.80) Remaining time to fill dewars is 3660 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 160 22 175 20, reference by 155 22 20 160 Trimming A by 160 22 175 20, reference by 165 20 175 20 Trimming A by 160 22 175 20, reference by 23 220 20 205 Trimming A by 160 22 175 20, reference by 23 254 210 20 Disparity in stage position after aligning to center of map frame: 0.07 0.01 Trimming A by 30 20 30 20, reference by 165 20 175 20 Disparity in stage position after aligning to target position: 0.10 -0.21 (2nd move error 0.02 -0.25) Finished aligning to item in 2 rounds Trimming A by 30 20 25 20, reference by 30 20 30 20 -27.0 0.2 unbinned pixels; 35.8 96.9 nm along two shift axes; 5.1 -105.0 nm on specimen axes Total_Shift = 53.945734 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -1.89 microns changed by -0.61 to target drift = 0.14 nm/sec Item 9: Stage: 196.15 -23.80 124 Label: 10 9 Tick time from program start = 87909.044 Running script 1 at item 11 (194.89, -20.42) Remaining time to fill dewars is 3480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 75 20 55 20, reference by 165 20 175 20 Disparity in stage position after aligning to target position: 0.09 -0.22 (2nd move error 0.01 -0.25) Finished aligning to item in 1 rounds Trimming A by 75 24 50 20, reference by 75 20 55 20 -29.2 2.2 unbinned pixels; 46.2 101.7 nm along two shift axes; -2.4 -114.0 nm on specimen axes Total_Shift = 58.511708 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.51 microns changed by -2.09 to target drift = 0.15 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.10 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot3_0001.tif Item 10: Stage: 194.89 -20.42 124 Label: 11 10 Tick time from program start = 88079.132 The original displacements in the overlap zones have mean 15.30 maximum 20.02 pixels. After shifting pieces into register, the displacements have mean 2.40 maximum 2.40 pixels. Running script 6 at item 6 (-738.80, -171.55) Trimming A by 80 85, reference by 85 85 Trimming A by 70 75, reference by 85 85 Trimming A by 45 45, reference by 85 85 Rough eucentricity: changing Z by -23.53 to 76.47, continuing... Trimming A by 30 30, reference by 30 35 Trimming A by 30 30, reference by 30 35 Rough eucentricity: changing Z by -1.25 to 75.23, finished. Z = 0: The original displacements in the overlap zones have mean 20.80 maximum 33.68 pixels. After shifting pieces into register, the displacements have mean 2.68 maximum 2.68 pixels. Running script 1 at item 8 (-738.49, -175.26) Remaining time to fill dewars is 2160 Dewars are NOT busy filling Trimming A by 24 107 150 20, reference by 255 22 20 205 Trimming A by 24 107 150 20, reference by 266 22 180 20 Trimming A by 24 107 150 20, reference by 20 110 20 140 Trimming A by 24 107 150 20, reference by 23 116 145 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.03 Trimming A by 20 20 20 20, reference by 23 116 145 20 Disparity in stage position after aligning to target position: 0.01 -0.32 (2nd move error -0.03 -0.31) Finished aligning to item in 2 rounds -1059.2 185.6 unbinned pixels; 45.3 78.8 nm along two shift axes; -8.0 -91.3 nm on specimen axes Total_Shift = 2150.756145 868.0 398.1 unbinned pixels; 7.0 81.2 nm along two shift axes; -40.1 70.8 nm on specimen axes Total_Shift = 1909.808112 Target defocus is: -2.60 um 0.00 degrees Running script 1 at item 8 (-738.49, -175.26) Remaining time to fill dewars is 2040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 116 145 20 Disparity in stage position after aligning to target position: 0.06 -0.31 (2nd move error 0.03 -0.29) Finished aligning to item in 1 rounds -35.6 1.9 unbinned pixels; 53.4 125.5 nm along two shift axes; 0.3 -139.0 nm on specimen axes Total_Shift = 71.379404 -7.4 0.2 unbinned pixels; 10.5 26.5 nm along two shift axes; 0.8 -29.0 nm on specimen axes Total_Shift = 14.898674 Target defocus is: -2.70 um 0.00 degrees Target defocus is: -2.80 um Measured defocus = -0.83 microns changed by -1.97 to target drift = 0.14 nm/sec Measured defocus = -2.91 microns changed by 0.11 to target drift = 0.10 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot4_0001.tif Item 8: Stage: -738.49 -175.26 75 Label: 8 8 Tick time from program start = 89455.466 Running script 1 at item 9 (-737.40, -179.00) Remaining time to fill dewars is 1920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 45 30 30, reference by 23 116 145 20 Disparity in stage position after aligning to target position: 0.12 -0.27 (2nd move error 0.09 -0.26) Finished aligning to item in 1 rounds Trimming A by 20 50 30 25, reference by 21 45 30 30 -27.3 1.4 unbinned pixels; 40.9 96.2 nm along two shift axes; 0.2 -106.6 nm on specimen axes Total_Shift = 54.700696 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Measured defocus = -1.07 microns changed by -1.83 to target drift = 0.17 nm/sec Measured defocus = -2.93 microns changed by 0.03 to target drift = 0.10 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot4_0002.tif Item 9: Stage: -737.40 -179.00 75 Label: 9 9 Tick time from program start = 89592.123 The original displacements in the overlap zones have mean 10.71 maximum 16.50 pixels. After shifting pieces into register, the displacements have mean 2.72 maximum 2.72 pixels. Running script 6 at item 6 (6.82, -241.35) Trimming A by 41 75, reference by 42 85 Trimming A by 27 65, reference by 42 85 Trimming A by 30 45, reference by 42 85 Rough eucentricity: changing Z by -44.41 to 55.59, continuing... Rough eucentricity: changing Z by -1.33 to 54.26, finished. Z = 0: The original displacements in the overlap zones have mean 25.74 maximum 34.07 pixels. After shifting pieces into register, the displacements have mean 0.51 maximum 0.51 pixels. Running script 1 at item 8 (11.76, -241.84) Remaining time to fill dewars is 240 Dewars are NOT busy filling Trimming A by 62 24 115 20, reference by 185 21 20 215 Trimming A by 62 24 115 20, reference by 75 22 105 20 Trimming A by 62 24 115 20, reference by 22 165 20 185 Trimming A by 62 24 115 20, reference by 22 77 180 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.04 Trimming A by 20 30 60 20, reference by 75 22 105 20 Disparity in stage position after aligning to target position: 0.15 -0.09 (2nd move error 0.09 -0.08) Finished aligning to item in 2 rounds Trimming A by 23 35 60 20, reference by 20 30 60 20 -7.3 -2.4 unbinned pixels; 0.7 29.8 nm along two shift axes; 10.8 -27.8 nm on specimen axes Total_Shift = 15.340136 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Measured defocus = -0.86 microns changed by -2.14 to target drift = 0.08 nm/sec Measured defocus = -3.03 microns changed by 0.03 to target drift = 0.05 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot6_0001.tif Item 8: Stage: 11.76 -241.84 54 Label: 8 8 Tick time from program start = 91196.375 Running script 1 at item 9 (7.56, -237.55) Remaining time to fill dewars is 120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 40 21 20 40, reference by 75 22 105 20 Disparity in stage position after aligning to target position: 0.14 -0.25 (2nd move error 0.08 -0.23) Finished aligning to item in 1 rounds Trimming A by 35 21 20 40, reference by 40 21 20 40 -29.2 -1.9 unbinned pixels; 31.1 108.1 nm along two shift axes; 13.7 -113.3 nm on specimen axes Total_Shift = 58.581248 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -3.10 um Measured defocus = -2.71 microns changed by -0.39 to target drift = 0.16 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot6_0002.tif Item 9: Stage: 7.56 -237.55 54 Label: 9 9 Tick time from program start = 91263.768 Running script 1 at item 10 (8.49, -245.32) Remaining time to fill dewars is -1860 Dewars ARE busy filling Dewars ARE busy filling Dewars are NOT busy filling Trimming A by 22 90 165 20, reference by 185 21 20 215 Trimming A by 22 90 165 20, reference by 75 22 105 20 Trimming A by 22 90 165 20, reference by 22 165 20 185 Trimming A by 22 90 165 20, reference by 22 77 180 20 Disparity in stage position after aligning to center of map frame: 0.24 -0.04 Trimming A by 20 20 20 20, reference by 22 77 180 20 Disparity in stage position after aligning to target position: 0.20 -0.29 (2nd move error -0.04 -0.28) Finished aligning to item in 2 rounds -37.0 10.5 unbinned pixels; 86.9 117.3 nm along two shift axes; -33.1 -146.1 nm on specimen axes Total_Shift = 76.900891 -7.8 6.4 unbinned pixels; 33.7 18.6 nm along two shift axes; -23.1 -31.9 nm on specimen axes Total_Shift = 20.21327 Target defocus is: -3.10 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.22 microns changed by -2.32 to target drift = 0.09 nm/sec Measured defocus = -2.07 microns changed by -0.03 to target drift = 0.06 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot6_0003.tif Item 10: Stage: 8.49 -245.32 54 Label: 10 10 Tick time from program start = 91811.331 Running script 1 at item 11 (8.32, -243.22) Remaining time to fill dewars is 31380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 22 77 180 20 Disparity in stage position after aligning to target position: 0.20 -0.31 (2nd move error -0.05 -0.29) Finished aligning to item in 1 rounds -34.1 10.5 unbinned pixels; 83.2 106.8 nm along two shift axes; -33.8 -134.8 nm on specimen axes Total_Shift = 71.336088 -5.0 6.4 unbinned pixels; 30.2 8.4 nm along two shift axes; -24.0 -20.9 nm on specimen axes Total_Shift = 16.335429 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.16 microns changed by -2.36 to target drift = 0.05 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.06 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot6_0004.tif Item 11: Stage: 8.32 -243.22 54 Label: 11 11 Tick time from program start = 91894.854 The original displacements in the overlap zones have mean 16.57 maximum 20.41 pixels. After shifting pieces into register, the displacements have mean 2.77 maximum 2.77 pixels. Running script 6 at item 6 (-673.10, 191.09) Rough eucentricity: changing Z by -53.60 to 46.40, continuing... Rough eucentricity: changing Z by -2.25 to 44.15, finished. Z = 0: The original displacements in the overlap zones have mean 19.75 maximum 31.95 pixels. After shifting pieces into register, the displacements have mean 0.36 maximum 0.71 pixels. Running script 1 at item 8 (-689.03, 193.59) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 230 23 20 175 Trimming A by 20 20 20 20, reference by 55 21 40 55 Disparity in stage position after aligning to center of map frame: 0.09 -0.01 Disparity in stage position after aligning to target position: 0.19 0.10 (2nd move error 0.10 0.09) Finished aligning to item in 2 rounds 2.2 -3.8 unbinned pixels; 16.9 2.1 nm along two shift axes; 14.4 9.2 nm on specimen axes Total_Shift = 8.788128 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.69 microns changed by -1.61 to target drift = 0.07 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target drift = 0.02 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot9_0001.tif Item 8: Stage: -689.03 193.59 44 Label: 8 8 Tick time from program start = 93307.552 Running script 1 at item 9 (-687.12, 196.57) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 0.19 (2nd move error 0.13 0.18) Finished aligning to item in 1 rounds 5.0 3.3 unbinned pixels; 5.4 23.0 nm along two shift axes; -13.7 18.8 nm on specimen axes Total_Shift = 11.969208 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.90 microns changed by -1.50 to target drift = 0.07 nm/sec Measured defocus = -2.45 microns changed by 0.06 to target drift = 0.04 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot9_0002.tif Item 9: Stage: -687.12 196.57 44 Label: 9 9 Tick time from program start = 93385.600 Running script 1 at item 10 (-684.49, 202.18) Remaining time to fill dewars is 30300 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 230 23 20 175 Trimming A by 20 20 20 20, reference by 55 21 40 55 Disparity in stage position after aligning to center of map frame: 0.19 -0.00 Disparity in stage position after aligning to target position: 0.15 -0.12 (2nd move error -0.05 -0.14) Finished aligning to item in 2 rounds -18.5 13.4 unbinned pixels; 73.4 46.5 nm along two shift axes; -48.2 -74.8 nm on specimen axes Total_Shift = 45.690691 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -1.52 microns changed by -0.98 to target drift = 0.08 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot9_0003.tif Item 10: Stage: -684.49 202.18 44 Label: 10 10 Tick time from program start = 93461.463 Running script 1 at item 11 (-696.10, 198.48) Remaining time to fill dewars is 30240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 21 20 40, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.10 -0.13 (2nd move error 0.01 -0.13) Finished aligning to item in 1 rounds Trimming A by 35 21 20 40, reference by 35 21 20 40 -15.5 0.2 unbinned pixels; 21.0 55.5 nm along two shift axes; 2.5 -60.3 nm on specimen axes Total_Shift = 31.002531 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -1.62 microns changed by -0.98 to target drift = 0.11 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot9_0004.tif Item 11: Stage: -696.10 198.48 44 Label: 11 11 Tick time from program start = 93529.745 Running script 1 at item 12 (-693.95, 201.45) Remaining time to fill dewars is 30240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 20 20 70, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.07 -0.06 (2nd move error -0.02 -0.06) Finished aligning to item in 1 rounds Trimming A by 65 20 20 70, reference by 65 20 20 70 -6.5 4.5 unbinned pixels; 25.0 16.9 nm along two shift axes; -16.0 -26.4 nm on specimen axes Total_Shift = 15.851052 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -2.70 um Measured defocus = -1.72 microns changed by -0.98 to target drift = 0.03 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot9_0005.tif Item 12: Stage: -693.95 201.45 44 Label: 12 12 Tick time from program start = 93596.591 The original displacements in the overlap zones have mean 86.82 maximum 295.09 pixels. After shifting pieces into register, the displacements have mean 68.19 maximum 68.19 pixels. Running script 6 at item 4 (-48.41, -686.73) Rough eucentricity: changing Z by 18.92 to 118.92, continuing... Rough eucentricity: changing Z by -0.87 to 118.05, finished. Z = 0: The original displacements in the overlap zones have mean 30.91 maximum 44.53 pixels. After shifting pieces into register, the displacements have mean 0.03 maximum 0.03 pixels. Running script 1 at item 6 (-48.31, -690.53) Remaining time to fill dewars is 29580 Dewars are NOT busy filling Trimming A by 22 86 20 130, reference by 175 21 80 135 Trimming A by 22 86 20 130, reference by 135 21 200 20 Trimming A by 22 86 20 130, reference by 21 81 20 130 Trimming A by 22 86 20 130, reference by 22 165 150 20 Disparity in stage position after aligning to center of map frame: 0.07 -0.00 Trimming A by 20 25 20 20, reference by 21 81 20 130 Disparity in stage position after aligning to target position: 0.12 0.41 (2nd move error 0.05 0.39) Finished aligning to item in 2 rounds Trimming A by 20 20 20 20, reference by 20 25 20 20 3.3 -4.8 unbinned pixels; 22.0 4.4 nm along two shift axes; 18.1 13.7 nm on specimen axes Total_Shift = 11.65128 Target defocus is: -2.70 um 0.00 degrees Target defocus is: -2.80 um Measured defocus = -0.81 microns changed by -1.99 to target drift = 0.06 nm/sec Measured defocus = -2.81 microns changed by 0.01 to target drift = 0.03 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot10_0001.tif Item 6: Stage: -48.31 -690.53 118 Label: 6 6 Tick time from program start = 94462.225 Running script 1 at item 7 (-48.47, -692.99) Remaining time to fill dewars is 29520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 80 55 20, reference by 21 81 20 130 Disparity in stage position after aligning to target position: 0.21 0.32 (2nd move error 0.14 0.31) Finished aligning to item in 1 rounds Trimming A by 20 70 55 20, reference by 24 80 55 20 10.5 -4.5 unbinned pixels; 30.1 30.9 nm along two shift axes; 15.2 41.6 nm on specimen axes Total_Shift = 22.739544 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Measured defocus = -0.82 microns changed by -2.08 to target drift = 0.09 nm/sec Measured defocus = -2.91 microns changed by 0.01 to target drift = 0.03 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot10_0002.tif Item 7: Stage: -48.47 -692.99 118 Label: 7 7 Tick time from program start = 94540.537 The original displacements in the overlap zones have mean 11.25 maximum 15.51 pixels. After shifting pieces into register, the displacements have mean 2.29 maximum 2.29 pixels. The original displacements in the overlap zones have mean 28.21 maximum 224.08 pixels. After shifting pieces into register, the displacements have mean 17.54 maximum 70.56 pixels. Running script 6 at item 12 (227.79, -222.45) Rough eucentricity: changing Z by 18.64 to 93.86, continuing... Rough eucentricity: changing Z by -1.22 to 92.64, finished. Z = 0: The original displacements in the overlap zones have mean 24.41 maximum 37.26 pixels. After shifting pieces into register, the displacements have mean 7.50 maximum 7.50 pixels. Running script 6 at item 13 (257.83, -166.82) Rough eucentricity: changing Z by 17.56 to 92.79, continuing... Rough eucentricity: changing Z by -1.36 to 91.43, finished. Z = 1: The original displacements in the overlap zones have mean 19.85 maximum 36.41 pixels. After shifting pieces into register, the displacements have mean 7.95 maximum 7.95 pixels. Running script 6 at item 14 (311.39, -198.84) Trimming A by 20 25, reference by 25 30 Trimming A by 20 20, reference by 25 30 Trimming A by 20 20, reference by 25 30 Rough eucentricity: changing Z by 16.45 to 91.69, continuing... Rough eucentricity: changing Z by -1.36 to 90.33, finished. Z = 2: The original displacements in the overlap zones have mean 29.88 maximum 37.32 pixels. After shifting pieces into register, the displacements have mean 5.00 maximum 5.00 pixels. Running script 6 at item 15 (282.17, -253.73) Rough eucentricity: changing Z by 17.46 to 92.69, continuing... Rough eucentricity: changing Z by -1.60 to 91.10, finished. Z = 3: The original displacements in the overlap zones have mean 25.17 maximum 38.27 pixels. After shifting pieces into register, the displacements have mean 2.00 maximum 2.00 pixels. Running script 6 at item 16 (252.13, -309.64) Rough eucentricity: changing Z by 18.09 to 93.32, continuing... Rough eucentricity: changing Z by -1.58 to 91.75, finished. Z = 4: The original displacements in the overlap zones have mean 21.67 maximum 35.59 pixels. After shifting pieces into register, the displacements have mean 15.50 maximum 15.50 pixels. Running script 6 at item 17 (197.79, -277.27) Rough eucentricity: changing Z by 19.69 to 94.93, continuing... Rough eucentricity: changing Z by -1.66 to 93.27, finished. Z = 5: The original displacements in the overlap zones have mean 30.58 maximum 39.50 pixels. After shifting pieces into register, the displacements have mean 2.80 maximum 2.80 pixels. Running script 6 at item 18 (335.53, -285.27) Rough eucentricity: changing Z by 15.91 to 91.14, finished. Z = 6: The original displacements in the overlap zones have mean 19.65 maximum 39.78 pixels. After shifting pieces into register, the displacements have mean 6.84 maximum 6.84 pixels. Running script 6 at item 19 (365.28, -230.15) Trimming A by 30 30, reference by 35 35 Trimming A by 25 25, reference by 35 35 Trimming A by 20 20, reference by 35 35 Trimming A by 20 20, reference by 35 35 Rough eucentricity: changing Z by 14.55 to 89.78, finished. Z = 7: The original displacements in the overlap zones have mean 32.88 maximum 51.62 pixels. After shifting pieces into register, the displacements have mean 22.98 maximum 22.98 pixels. Running script 6 at item 20 (389.98, -314.51) Trimming A by 30 35, reference by 20 20 Rough eucentricity: changing Z by 13.89 to 89.12, finished. Ending Navigator acquisition after current item Z = 8: The original displacements in the overlap zones have mean 17.89 maximum 31.12 pixels. After shifting pieces into register, the displacements have mean 8.28 maximum 8.28 pixels. Rough eucentricity: changing Z by 5.43 to 94.56, finished. Running script 3 at item 43 (-212.55, -263.09) Z = 9: The original displacements in the overlap zones have mean 21.06 maximum 31.59 pixels. After shifting pieces into register, the displacements have mean 1.06 maximum 1.06 pixels. Rough eucentricity: changing Z by 18.67 to 93.91, continuing... Rough eucentricity: changing Z by -1.18 to 92.72, finished. Full View shift offset is set to 0.154, -0.087 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to 0.174, -0.101 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Measured defocus = 0.51 microns drift = 0.05 nm/sec Changing defocus to -0.90 to get an acceptable number of CTF rings Ctffind: defocus: -0.952, astig: 0.046 um, angle: 51.6, score 0.2649, fit to 3.2 A Objective stigmator was adjusted by -0.0011 -0.0005 Changing defocus to -0.90 to get an acceptable number of CTF rings Ctffind: defocus: -0.925, astig: 0.004 um, angle: -69.0, score 0.6934, fit to 3.0 A Objective stigmator was adjusted by 0.0000 0.0001 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.488, astig: 0.008 um, angle: -56.2, score 0.7689, fit to 3.3 A Ctffind: defocus: -1.126, astig: 0.395 um, angle: 65.9, score 0.7390, fit to 2.9 A Ctffind: defocus: -1.076, astig: 0.444 um, angle: -71.9, score 0.7717, fit to 2.7 A Ctffind: defocus: -1.055, astig: 0.466 um, angle: -30.9, score 0.7723, fit to 2.5 A Ctffind: defocus: -1.084, astig: 0.445 um, angle: 9.8, score 0.7683, fit to 2.7 A Ctffind: defocus: -1.152, astig: 0.375 um, angle: 52.5, score 0.7864, fit to 2.7 A Ctffind: defocus: -1.220, astig: 0.318 um, angle: -80.9, score 0.7788, fit to 3.0 A Ctffind: defocus: -1.243, astig: 0.286 um, angle: -30.5, score 0.7702, fit to 2.9 A Ctffind: defocus: -1.210, astig: 0.321 um, angle: 20.5, score 0.7763, fit to 2.8 A Ctffind: defocus: -1.531, astig: 0.008 um, angle: -50.2, score 0.7764, fit to 3.4 A Beam tilt was adjusted by 0.11 1.06 milliradians Iterating because beam tilt is above threshold Ctffind: defocus: -1.538, astig: 0.005 um, angle: -67.6, score 0.7734, fit to 3.3 A Ctffind: defocus: -1.165, astig: 0.378 um, angle: 58.4, score 0.7745, fit to 2.9 A Ctffind: defocus: -1.176, astig: 0.361 um, angle: -76.3, score 0.7820, fit to 2.9 A Ctffind: defocus: -1.174, astig: 0.363 um, angle: -30.1, score 0.7811, fit to 2.8 A Ctffind: defocus: -1.165, astig: 0.376 um, angle: 15.2, score 0.7819, fit to 2.9 A Ctffind: defocus: -1.158, astig: 0.380 um, angle: 60.2, score 0.7843, fit to 2.7 A Ctffind: defocus: -1.156, astig: 0.390 um, angle: -75.7, score 0.7824, fit to 2.9 A Ctffind: defocus: -1.155, astig: 0.381 um, angle: -31.4, score 0.7780, fit to 2.7 A Ctffind: defocus: -1.158, astig: 0.379 um, angle: 13.9, score 0.7782, fit to 2.9 A Ctffind: defocus: -1.541, astig: 0.005 um, angle: -74.7, score 0.7770, fit to 3.4 A Beam tilt was adjusted by -0.04 -0.10 milliradians Changing defocus to -0.90 to get an acceptable number of CTF rings Ctffind: defocus: -0.922, astig: 0.010 um, angle: 32.7, score 0.6821, fit to 3.0 A Objective stigmator was adjusted by -0.0001 -0.0002 Measuring at image shift -1.1 0.0 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.480, astig: 0.054 um, angle: -71.1, score 0.5016, fit to 3.5 A Ctffind: defocus: -1.094, astig: 0.407 um, angle: 64.6, score 0.4996, fit to 4.3 A Ctffind: defocus: -0.995, astig: 0.211 um, angle: -85.7, score 0.1355, fit to 4.7 A Measuring at image shift -1.1 0.0 Measured defocus = 0.39 microns drift = 0.01 nm/sec Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.456, astig: 0.059 um, angle: -73.2, score 0.6276, fit to 3.0 A Ctffind: defocus: -1.063, astig: 0.414 um, angle: 63.9, score 0.5997, fit to 2.8 A Ctffind: defocus: -1.125, astig: 0.343 um, angle: 62.9, score 0.6433, fit to 2.7 A Ctffind: defocus: -1.097, astig: 0.387 um, angle: -36.0, score 0.6206, fit to 2.5 A Ctffind: defocus: -1.109, astig: 0.371 um, angle: -32.7, score 0.6138, fit to 2.5 A Beam tilt needs to be adjusted by 0.34 0.08 milliradians Measuring at image shift 1.1 0.0 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.533, astig: 0.039 um, angle: 10.0, score 0.6646, fit to 3.1 A Ctffind: defocus: -1.165, astig: 0.378 um, angle: 54.3, score 0.6777, fit to 2.5 A Ctffind: defocus: -1.154, astig: 0.375 um, angle: 57.8, score 0.6608, fit to 2.5 A Ctffind: defocus: -1.194, astig: 0.351 um, angle: -26.2, score 0.6451, fit to 2.8 A Ctffind: defocus: -1.133, astig: 0.413 um, angle: -28.4, score 0.6534, fit to 2.8 A Beam tilt needs to be adjusted by -0.07 -0.32 milliradians Measuring at image shift 0.0 -1.1 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.502, astig: 0.009 um, angle: 21.3, score 0.7037, fit to 3.2 A Ctffind: defocus: -1.098, astig: 0.419 um, angle: 56.7, score 0.7045, fit to 2.9 A Ctffind: defocus: -1.157, astig: 0.350 um, angle: 60.6, score 0.7173, fit to 2.8 A Ctffind: defocus: -1.163, astig: 0.338 um, angle: -31.3, score 0.7100, fit to 2.7 A Ctffind: defocus: -1.101, astig: 0.402 um, angle: -27.9, score 0.7075, fit to 2.6 A Beam tilt needs to be adjusted by 0.30 -0.34 milliradians Measuring at image shift 0.0 1.1 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.500, astig: 0.027 um, angle: -73.0, score 0.3045, fit to 4.0 A Ctffind: defocus: -1.132, astig: 0.370 um, angle: 61.8, score 0.2668, fit to 3.9 A Ctffind: defocus: -1.124, astig: 0.379 um, angle: 60.4, score 0.3130, fit to 4.0 A Ctffind: defocus: -1.255, astig: 0.099 um, angle: -8.1, score 0.0881, fit to 5.1 A Measuring at image shift -1.1 0.0 Measured defocus = 0.58 microns drift = 0.09 nm/sec Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.580, astig: 0.057 um, angle: -69.3, score 0.5433, fit to 3.8 A Ctffind: defocus: -1.206, astig: 0.400 um, angle: 64.8, score 0.5995, fit to 3.3 A Ctffind: defocus: -1.248, astig: 0.352 um, angle: 61.8, score 0.6440, fit to 3.0 A Ctffind: defocus: -1.221, astig: 0.400 um, angle: -35.1, score 0.6369, fit to 3.1 A Ctffind: defocus: -1.260, astig: 0.364 um, angle: -33.8, score 0.6345, fit to 2.9 A Beam tilt needs to be adjusted by 0.25 0.24 milliradians Measuring at image shift 1.1 0.0 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.635, astig: 0.032 um, angle: 10.0, score 0.4073, fit to 3.5 A Ctffind: defocus: -1.280, astig: 0.370 um, angle: 55.5, score 0.4380, fit to 3.1 A Ctffind: defocus: -1.248, astig: 0.381 um, angle: 57.5, score 0.3926, fit to 3.5 A Ctffind: defocus: -1.290, astig: 0.359 um, angle: -26.1, score 0.3767, fit to 3.9 A Ctffind: defocus: -1.256, astig: 0.399 um, angle: -29.2, score 0.4164, fit to 3.9 A Beam tilt needs to be adjusted by -0.19 -0.18 milliradians Measuring at image shift 0.0 -1.1 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.608, astig: 0.004 um, angle: 21.8, score 0.6288, fit to 3.2 A Ctffind: defocus: -1.219, astig: 0.401 um, angle: 57.7, score 0.6242, fit to 2.9 A Ctffind: defocus: -1.255, astig: 0.359 um, angle: 59.6, score 0.6258, fit to 2.5 A Ctffind: defocus: -1.263, astig: 0.354 um, angle: -30.7, score 0.6359, fit to 2.9 A Ctffind: defocus: -1.226, astig: 0.392 um, angle: -29.2, score 0.6164, fit to 2.9 A Beam tilt needs to be adjusted by 0.19 -0.20 milliradians Measuring at image shift 0.0 1.1 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.644, astig: 0.032 um, angle: -49.9, score 0.5718, fit to 3.4 A Ctffind: defocus: -1.286, astig: 0.352 um, angle: 62.8, score 0.5688, fit to 3.1 A Ctffind: defocus: -1.251, astig: 0.379 um, angle: 59.1, score 0.5811, fit to 2.9 A Ctffind: defocus: -1.258, astig: 0.405 um, angle: -30.1, score 0.5630, fit to 3.1 A Ctffind: defocus: -1.293, astig: 0.378 um, angle: -33.8, score 0.5952, fit to 3.0 A Beam tilt needs to be adjusted by -0.19 0.19 milliradians IS to beam tilt matrix: -0.198061 -0.174371 -0.188801 0.179353 Running script 1 at item 49 (-216.49, -412.20) Remaining time to fill dewars is 24900 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Running script 1 at item 50 (-206.97, -259.97) Remaining time to fill dewars is 24900 Dewars are NOT busy filling Trimming A by 205 22 155 20, reference by 160 22 20 170 Trimming A by 205 22 155 20, reference by 195 23 150 20 Trimming A by 205 22 155 20, reference by 21 120 20 125 Trimming A by 205 22 155 20, reference by 23 104 155 20 Disparity in stage position after aligning to center of map frame: -0.00 -0.17 Trimming A by 95 24 85 20, reference by 195 23 150 20 Disparity in stage position after aligning to target position: 0.08 -0.29 (2nd move error 0.07 -0.14) Finished aligning to item in 2 rounds Trimming A by 90 24 90 20, reference by 95 24 85 20 -18.4 1.7 unbinned pixels; 30.3 63.8 nm along two shift axes; -2.7 -72.1 nm on specimen axes Total_Shift = 37.040473 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Measured defocus = -1.17 microns changed by -1.83 to target drift = 0.18 nm/sec Measured defocus = -3.02 microns changed by 0.02 to target drift = 0.12 nm/sec 24 frames are being saved to X:\DoseFractions\20190717\slot4_0011.tif Item 47: Stage: -206.97 -259.97 95 Label: 50 47 Tick time from program start = 100015.159 Running script 17 at item 48 (-214.18, -255.64) Remaining time to fill dewars is 24600 Dewars are NOT busy filling Trimming A by 170 21 20 175, reference by 160 22 20 170 Trimming A by 170 21 20 175, reference by 195 23 150 20 Trimming A by 170 21 20 175, reference by 21 120 20 125 Trimming A by 170 21 20 175, reference by 23 104 155 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.18 Trimming A by 120 20 45 60, reference by 160 22 20 170 Disparity in stage position after aligning to target position: 0.10 0.06 (2nd move error 0.05 0.22) Finished aligning to item in 2 rounds Trimming A by 130 20 45 55, reference by 120 20 45 60 -5.6 -0.4 unbinned pixels; 5.9 20.9 nm along two shift axes; 2.7 -21.9 nm on specimen axes Total_Shift = 11.323943 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Measured defocus = 0.61 microns changed by -3.41 to target drift = 0.12 nm/sec Measured defocus = -2.88 microns changed by 0.08 to target drift = 0.09 nm/sec 27 frames were saved to X:\DoseFractions\20190717\slot4_0012.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0013.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0014.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0015.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0016.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0017.tif Drawn line is 135.0 pixels, 2.104 um at -44.5 degrees 27 frames were saved to X:\DoseFractions\20190717\slot4_0018.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0019.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0020.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0021.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0022.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0023.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0024.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0025.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0026.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0027.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0028.tif 27 frames were saved to X:\DoseFractions\20190717\slot4_0029.tif Running script 6 at item 21 (443.20, -345.06) Rough eucentricity: changing Z by 11.89 to 87.12, finished. Z = 0: The original displacements in the overlap zones have mean 12.57 maximum 19.91 pixels. After shifting pieces into register, the displacements have mean 4.17 maximum 4.17 pixels. Running script 6 at item 22 (463.93, -430.70) Trimming A by 25 25, reference by 20 25 Trimming A by 30 30, reference by 20 25 Trimming A by 40 40, reference by 20 25 Trimming A by 50 50, reference by 20 25 Rough eucentricity: changing Z by 9.91 to 85.14, finished. Z = 1: The original displacements in the overlap zones have mean 12.85 maximum 23.16 pixels. After shifting pieces into register, the displacements have mean 1.80 maximum 1.80 pixels. Running script 6 at item 23 (496.98, -378.09) Trimming A by 25 25, reference by 25 30 Trimming A by 20 20, reference by 25 30 Trimming A by 20 20, reference by 25 30 Trimming A by 20 20, reference by 25 30 Rough eucentricity: changing Z by 9.26 to 84.49, finished. Z = 2: The original displacements in the overlap zones have mean 8.54 maximum 14.71 pixels. After shifting pieces into register, the displacements have mean 6.29 maximum 6.29 pixels. Running script 6 at item 24 (517.75, -461.95) Trimming A by 20 25, reference by 20 20 Trimming A by 25 30, reference by 20 20 Rough eucentricity: changing Z by 6.73 to 81.96, finished. Z = 3: The original displacements in the overlap zones have mean 28.18 maximum 39.53 pixels. After shifting pieces into register, the displacements have mean 9.36 maximum 9.36 pixels. Running script 6 at item 25 (488.23, -517.91) Rough eucentricity: changing Z by 6.76 to 81.99, finished. Z = 4: The original displacements in the overlap zones have mean 21.49 maximum 36.50 pixels. After shifting pieces into register, the displacements have mean 9.50 maximum 9.50 pixels. Running script 6 at item 26 (540.60, -550.70) Trimming A by 45 50, reference by 45 50 Trimming A by 45 45, reference by 45 50 Trimming A by 40 45, reference by 45 50 Trimming A by 40 40, reference by 45 50 Rough eucentricity: changing Z by 2.72 to 77.95, finished. Z = 5: The original displacements in the overlap zones have mean 26.11 maximum 46.53 pixels. After shifting pieces into register, the displacements have mean 14.36 maximum 14.36 pixels. Running script 6 at item 27 (602.06, -435.81) Trimming A by 60 65, reference by 50 70 Trimming A by 60 65, reference by 50 70 Trimming A by 65 65, reference by 55 65 Trimming A by 65 70, reference by 50 70 Rough eucentricity: changing Z by 3.53 to 78.76, finished. Z = 6: The original displacements in the overlap zones have mean 16.61 maximum 32.60 pixels. After shifting pieces into register, the displacements have mean 6.27 maximum 6.27 pixels. Running script 6 at item 28 (580.61, -351.76) Trimming A by 30 35, reference by 35 35 Trimming A by 30 30, reference by 35 35 Trimming A by 25 30, reference by 30 35 Trimming A by 30 30, reference by 35 35 Rough eucentricity: changing Z by 6.38 to 81.61, finished. Z = 7: The original displacements in the overlap zones have mean 31.55 maximum 49.70 pixels. After shifting pieces into register, the displacements have mean 9.31 maximum 9.31 pixels. Running script 6 at item 29 (175.33, -194.38) Trimming A by 25 25, reference by 25 40 Trimming A by 20 20, reference by 25 40 Trimming A by 20 20, reference by 25 40 Rough eucentricity: changing Z by 19.19 to 94.43, continuing... Rough eucentricity: changing Z by -1.40 to 93.02, finished. Z = 8: The original displacements in the overlap zones have mean 19.09 maximum 34.59 pixels. After shifting pieces into register, the displacements have mean 12.11 maximum 12.11 pixels. Running script 6 at item 30 (91.16, -219.30) Trimming A by 20 20, reference by 25 25 Trimming A by 20 20, reference by 25 25 Trimming A by 20 20, reference by 25 25 Rough eucentricity: changing Z by 20.77 to 96.01, continuing... Rough eucentricity: changing Z by -1.56 to 94.45, finished. Z = 9: The original displacements in the overlap zones have mean 19.09 maximum 28.54 pixels. After shifting pieces into register, the displacements have mean 13.77 maximum 13.77 pixels. Running script 6 at item 31 (6.93, -243.54) Rough eucentricity: changing Z by 21.05 to 96.28, continuing... Rough eucentricity: changing Z by -1.52 to 94.77, finished. Z = 10: The original displacements in the overlap zones have mean 23.28 maximum 34.68 pixels. After shifting pieces into register, the displacements have mean 0.93 maximum 0.93 pixels. Running script 6 at item 32 (-47.12, -214.19) Trimming A by 20 20, reference by 25 25 Trimming A by 20 20, reference by 25 25 Trimming A by 20 20, reference by 25 25 Rough eucentricity: changing Z by 20.76 to 96.00, continuing... Rough eucentricity: changing Z by -1.61 to 94.39, finished. Z = 11: The original displacements in the overlap zones have mean 16.24 maximum 24.01 pixels. After shifting pieces into register, the displacements have mean 13.40 maximum 13.40 pixels. Running script 6 at item 229 (-404.45, -226.60) Rough eucentricity: changing Z by 13.97 to 89.21, finished. Z = 0: The original displacements in the overlap zones have mean 22.73 maximum 32.75 pixels. After shifting pieces into register, the displacements have mean 0.64 maximum 0.64 pixels. Running script 6 at item 230 (-260.90, -90.87) Rough eucentricity: changing Z by 16.63 to 91.87, continuing... Rough eucentricity: changing Z by -1.22 to 90.64, finished. Z = 1: The original displacements in the overlap zones have mean 15.56 maximum 31.50 pixels. After shifting pieces into register, the displacements have mean 8.31 maximum 8.31 pixels. Running script 6 at item 231 (-128.92, -238.20) Rough eucentricity: changing Z by 20.03 to 95.27, continuing... Rough eucentricity: changing Z by -1.22 to 94.04, finished. Z = 2: The original displacements in the overlap zones have mean 20.61 maximum 36.09 pixels. After shifting pieces into register, the displacements have mean 13.88 maximum 13.88 pixels. Running script 6 at item 232 (-181.59, -719.21) Rough eucentricity: changing Z by 14.45 to 89.69, finished. Z = 3: The original displacements in the overlap zones have mean 26.38 maximum 63.55 pixels. After shifting pieces into register, the displacements have mean 17.90 maximum 17.90 pixels. Running script 6 at item 233 (-258.23, -604.77) Rough eucentricity: changing Z by 14.04 to 89.28, finished. Z = 4: The original displacements in the overlap zones have mean 23.69 maximum 44.03 pixels. After shifting pieces into register, the displacements have mean 6.73 maximum 6.73 pixels. Running script 6 at item 234 (-619.80, -105.37) Rough eucentricity: changing Z by 6.41 to 81.64, finished. Z = 5: The original displacements in the overlap zones have mean 9.96 maximum 14.51 pixels. After shifting pieces into register, the displacements have mean 2.29 maximum 2.29 pixels. Running script 6 at item 235 (-763.44, -243.76) Rough eucentricity: changing Z by -1.58 to 73.65, finished. Z = 6: The original displacements in the overlap zones have mean 19.46 maximum 30.70 pixels. After shifting pieces into register, the displacements have mean 8.39 maximum 8.39 pixels. Running script 6 at item 236 (-733.21, -187.28) Rough eucentricity: changing Z by 1.32 to 76.54, finished. Z = 7: The original displacements in the overlap zones have mean 20.09 maximum 31.95 pixels. After shifting pieces into register, the displacements have mean 2.18 maximum 2.18 pixels. Running script 6 at item 237 (-787.63, -156.46) Rough eucentricity: changing Z by -1.04 to 74.19, finished. Z = 8: The original displacements in the overlap zones have mean 20.55 maximum 32.96 pixels. After shifting pieces into register, the displacements have mean 0.30 maximum 0.30 pixels. Running script 6 at item 238 (-727.92, -44.17) Rough eucentricity: changing Z by 2.48 to 77.70, finished. Z = 9: The original displacements in the overlap zones have mean 19.81 maximum 30.44 pixels. After shifting pieces into register, the displacements have mean 9.85 maximum 9.85 pixels. Running script 6 at item 239 (-452.70, -56.03) Trimming A by 20 20, reference by 20 25 Trimming A by 20 20, reference by 20 25 Trimming A by 20 20, reference by 20 25 Trimming A by 20 20, reference by 20 25 Rough eucentricity: changing Z by 12.11 to 87.34, finished. Ending Navigator acquisition after current item Z = 10: The original displacements in the overlap zones have mean 13.82 maximum 16.89 pixels. After shifting pieces into register, the displacements have mean 8.08 maximum 8.08 pixels. Running script 17 at item 51-1 (235.62, -221.91) Remaining time to fill dewars is 21120 Dewars are NOT busy filling Trimming A by 24 115 125 20, reference by 227 20 20 240 Trimming A by 24 115 125 20, reference by 241 20 165 20 Trimming A by 24 115 125 20, reference by 22 110 20 155 Trimming A by 24 115 125 20, reference by 22 120 120 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.25 Trimming A by 100 20 130 20, reference by 22 120 120 20 Disparity in stage position after aligning to target position: -0.03 -0.15 (2nd move error -0.10 0.08) Finished aligning to item in 2 rounds Trimming A by 100 20 130 20, reference by 100 20 130 20 4.4 18.9 unbinned pixels; 63.8 44.2 nm along two shift axes; -74.5 13.0 nm on specimen axes Total_Shift = 38.818261 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = -0.57 microns changed by -0.93 to target drift = 0.14 nm/sec 27 frames were saved to U:\krios\20190717\PilA_0001.tif 27 frames were saved to U:\krios\20190717\PilA_0002.tif 27 frames were saved to U:\krios\20190717\PilA_0003.tif 27 frames were saved to U:\krios\20190717\PilA_0004.tif 27 frames were saved to U:\krios\20190717\PilA_0005.tif Ending Navigator acquisition after current item 27 frames were saved to U:\krios\20190717\PilA_0006.tif Measured defocus = -0.18 microns drift = 0.02 nm/sec The current defocus is computed to be -0.18 microns Running script 17 at item 51-1 (235.62, -221.91) Remaining time to fill dewars is 20820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 100 20 130 20, reference by 22 120 120 20 Disparity in stage position after aligning to target position: -0.00 -0.17 (2nd move error -0.07 0.06) Finished aligning to item in 1 rounds Trimming A by 100 20 130 20, reference by 100 20 130 20 2.6 13.8 unbinned pixels; 47.3 29.9 nm along two shift axes; -54.1 7.0 nm on specimen axes Total_Shift = 28.020208 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.63 microns changed by -2.23 to target drift = 0.10 nm/sec Measured defocus = -1.58 microns changed by -0.02 to target drift = 0.03 nm/sec 27 frames were saved to U:\krios\20190717\PilA_0007.tif 27 frames were saved to U:\krios\20190717\PilA_0008.tif 27 frames were saved to U:\krios\20190717\PilA_0009.tif 27 frames were saved to U:\krios\20190717\PilA_0010.tif 27 frames were saved to U:\krios\20190717\PilA_0011.tif 27 frames were saved to U:\krios\20190717\PilA_0012.tif 27 frames were saved to U:\krios\20190717\PilA_0013.tif Min = 3205.0, max = 7757.0, mean = 5208.35, SD = 391.74 Pixel size = 0.170 nm 7.750 electrons (241.13 counts) per unbinned pixel per second Running script 17 at item 52-1 (232.84, -220.23) Remaining time to fill dewars is 20580 Dewars are NOT busy filling Trimming A by 250 20 170 20, reference by 227 20 20 240 Trimming A by 250 20 170 20, reference by 241 20 165 20 Trimming A by 250 20 170 20, reference by 22 110 20 155 Trimming A by 250 20 170 20, reference by 22 120 120 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.19 Trimming A by 110 22 100 20, reference by 241 20 165 20 Disparity in stage position after aligning to target position: 0.29 -0.34 (2nd move error 0.14 -0.17) Finished aligning to item in 2 rounds Trimming A by 110 23 100 20, reference by 110 22 100 20 -19.4 -1.9 unbinned pixels; 18.3 72.6 nm along two shift axes; 11.5 -74.9 nm on specimen axes Total_Shift = 38.915155 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.49 microns changed by -2.19 to target drift = 0.16 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames were saved to U:\krios\20190717\PilA_0014.tif 27 frames were saved to U:\krios\20190717\PilA_0015.tif 27 frames were saved to U:\krios\20190717\PilA_0016.tif 27 frames were saved to U:\krios\20190717\PilA_0017.tif 27 frames were saved to U:\krios\20190717\PilA_0018.tif 27 frames were saved to U:\krios\20190717\PilA_0019.tif Running script 17 at item 53-1 (230.41, -218.54) Remaining time to fill dewars is 20280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Ending Navigator acquisition after current item Trimming A by 125 24 85 20, reference by 241 20 165 20 Disparity in stage position after aligning to target position: 0.22 -0.38 (2nd move error 0.07 -0.21) Finished aligning to item in 1 rounds Trimming A by 120 24 85 20, reference by 125 24 85 20 -27.4 -2.0 unbinned pixels; 28.4 101.8 nm along two shift axes; 13.7 -106.3 nm on specimen axes Total_Shift = 55.030353 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.43 microns changed by -2.23 to target drift = 0.16 nm/sec Measured defocus = -1.89 microns changed by 0.09 to target drift = 0.13 nm/sec 27 frames were saved to U:\krios\20190717\PilA_0020.tif 27 frames were saved to U:\krios\20190717\PilA_0021.tif 27 frames were saved to U:\krios\20190717\PilA_0022.tif 27 frames were saved to U:\krios\20190717\PilA_0023.tif 27 frames were saved to U:\krios\20190717\PilA_0024.tif 27 frames were saved to U:\krios\20190717\PilA_0025.tif Running script 17 at item 54-1 (227.88, -216.83) Remaining time to fill dewars is 19620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 20 60 70, reference by 241 20 165 20 Disparity in stage position after aligning to target position: 0.15 -0.49 (2nd move error 0.01 -0.32) Finished aligning to item in 1 rounds Trimming A by 140 20 65 70, reference by 150 20 60 70 -31.6 2.4 unbinned pixels; 50.1 110.0 nm along two shift axes; -2.7 -123.3 nm on specimen axes Total_Shift = 63.317981 Trimming A by 140 20 60 70, reference by 150 20 60 70 -5.0 1.2 unbinned pixels; 10.8 16.4 nm along two shift axes; -3.4 -19.8 nm on specimen axes Total_Shift = 10.334335 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.28 microns changed by -2.18 to target drift = 0.11 nm/sec Measured defocus = -1.88 microns changed by -0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0026.tif 27 frames are being saved to U:\krios\20190717\PilA_0027.tif 27 frames are being saved to U:\krios\20190717\PilA_0028.tif 27 frames are being saved to U:\krios\20190717\PilA_0029.tif 27 frames are being saved to U:\krios\20190717\PilA_0030.tif 27 frames are being saved to U:\krios\20190717\PilA_0031.tif Running script 17 at item 55-1 (226.07, -219.98) Remaining time to fill dewars is 19440 Dewars are NOT busy filling Trimming A by 250 20 20 235, reference by 227 20 20 240 Trimming A by 250 20 20 235, reference by 241 20 165 20 Trimming A by 250 20 20 235, reference by 22 110 20 155 Trimming A by 250 20 20 235, reference by 22 120 120 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.14 Trimming A by 95 20 20 85, reference by 227 20 20 240 Disparity in stage position after aligning to target position: 0.34 0.12 (2nd move error 0.20 0.23) Finished aligning to item in 2 rounds Trimming A by 95 20 20 85, reference by 95 20 20 85 5.0 6.5 unbinned pixels; 17.3 27.6 nm along two shift axes; -26.2 17.9 nm on specimen axes Total_Shift = 16.285317 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.07 microns changed by -2.07 to target drift = 0.16 nm/sec Measured defocus = -1.94 microns changed by -0.06 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0032.tif 27 frames are being saved to U:\krios\20190717\PilA_0033.tif 27 frames are being saved to U:\krios\20190717\PilA_0034.tif 27 frames are being saved to U:\krios\20190717\PilA_0035.tif 27 frames are being saved to U:\krios\20190717\PilA_0036.tif 27 frames are being saved to U:\krios\20190717\PilA_0037.tif Running script 17 at item 56-1 (223.67, -218.24) Remaining time to fill dewars is 19260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 160 22 20 150, reference by 227 20 20 240 Disparity in stage position after aligning to target position: 0.24 -0.03 (2nd move error 0.10 0.08) Finished aligning to item in 1 rounds Trimming A by 160 23 20 150, reference by 160 22 20 150 1.3 -0.2 unbinned pixels; 2.5 4.2 nm along two shift axes; 0.6 4.9 nm on specimen axes Total_Shift = 2.545008 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 3.86 microns changed by -5.96 to target drift = 2.00 nm/sec Measured defocus = -6.15 microns changed by 4.06 to target drift = 0.09 nm/sec Measured defocus = -3.99 microns changed by 1.89 to target drift = 0.06 nm/sec Measured defocus = -2.25 microns changed by 0.15 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0038.tif 27 frames are being saved to U:\krios\20190717\PilA_0039.tif 27 frames are being saved to U:\krios\20190717\PilA_0040.tif 27 frames are being saved to U:\krios\20190717\PilA_0041.tif 27 frames are being saved to U:\krios\20190717\PilA_0042.tif 27 frames are being saved to U:\krios\20190717\PilA_0043.tif Running script 17 at item 57-1 (228.70, -221.13) Remaining time to fill dewars is 19080 Dewars are NOT busy filling Trimming A by 255 20 170 20, reference by 227 20 20 240 Trimming A by 255 20 170 20, reference by 241 20 165 20 Trimming A by 255 20 170 20, reference by 22 110 20 155 Trimming A by 255 20 170 20, reference by 22 120 120 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.19 Trimming A by 50 20 20 20, reference by 241 20 165 20 Disparity in stage position after aligning to target position: 0.22 -0.44 (2nd move error 0.09 -0.28) Finished aligning to item in 2 rounds Trimming A by 50 20 20 20, reference by 50 20 20 20 -27.9 -2.1 unbinned pixels; 28.9 103.6 nm along two shift axes; 13.9 -108.2 nm on specimen axes Total_Shift = 56.001294 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.01 microns changed by -2.21 to target drift = 0.19 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0044.tif 27 frames are being saved to U:\krios\20190717\PilA_0045.tif 27 frames are being saved to U:\krios\20190717\PilA_0046.tif 27 frames are being saved to U:\krios\20190717\PilA_0047.tif 27 frames are being saved to U:\krios\20190717\PilA_0048.tif 27 frames are being saved to U:\krios\20190717\PilA_0049.tif Running script 17 at item 58-1 (231.49, -222.72) Remaining time to fill dewars is 18900 Dewars are NOT busy filling Trimming A by 22 102 135 20, reference by 227 20 20 240 Trimming A by 22 102 135 20, reference by 241 20 165 20 Trimming A by 22 102 135 20, reference by 22 110 20 155 Trimming A by 22 102 135 20, reference by 22 120 120 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.23 Trimming A by 45 20 40 20, reference by 22 120 120 20 Disparity in stage position after aligning to target position: -0.05 -0.36 (2nd move error -0.18 -0.15) Finished aligning to item in 2 rounds Trimming A by 40 21 40 20, reference by 45 20 40 20 -16.9 35.7 unbinned pixels; 153.3 7.2 nm along two shift axes; -135.3 -73.3 nm on specimen axes Total_Shift = 79.003268 Trimming A by 40 21 40 20, reference by 45 20 40 20 -4.9 24.1 unbinned pixels; 95.1 18.8 nm along two shift axes; -92.8 -24.0 nm on specimen axes Total_Shift = 49.217526 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.06 microns changed by -2.24 to target drift = 0.02 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0050.tif 27 frames are being saved to U:\krios\20190717\PilA_0051.tif 27 frames are being saved to U:\krios\20190717\PilA_0052.tif 27 frames are being saved to U:\krios\20190717\PilA_0053.tif 27 frames are being saved to U:\krios\20190717\PilA_0054.tif 27 frames are being saved to U:\krios\20190717\PilA_0055.tif Running script 17 at item 59-1 (234.02, -224.51) Remaining time to fill dewars is 18660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 36 95 20, reference by 22 120 120 20 Disparity in stage position after aligning to target position: 0.07 -0.25 (2nd move error -0.06 -0.04) Finished aligning to item in 1 rounds Trimming A by 22 27 100 20, reference by 22 36 95 20 -3.7 12.3 unbinned pixels; 49.9 5.1 nm along two shift axes; -46.9 -17.0 nm on specimen axes Total_Shift = 25.619685 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.19 microns changed by -2.21 to target drift = 0.04 nm/sec Measured defocus = -2.46 microns changed by 0.06 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0056.tif 27 frames are being saved to U:\krios\20190717\PilA_0057.tif 27 frames are being saved to U:\krios\20190717\PilA_0058.tif 27 frames are being saved to U:\krios\20190717\PilA_0059.tif 27 frames are being saved to U:\krios\20190717\PilA_0060.tif 27 frames are being saved to U:\krios\20190717\PilA_0061.tif Running script 17 at item 60-1 (232.32, -227.09) Remaining time to fill dewars is 18480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 51 75 20, reference by 22 120 120 20 Disparity in stage position after aligning to target position: 0.14 -0.37 (2nd move error 0.00 -0.17) Finished aligning to item in 1 rounds Trimming A by 23 47 80 20, reference by 20 51 75 20 -22.2 1.6 unbinned pixels; 34.8 77.6 nm along two shift axes; -1.4 -86.8 nm on specimen axes Total_Shift = 44.53913 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.32 microns changed by -2.18 to target drift = 0.09 nm/sec Measured defocus = -2.52 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0062.tif 27 frames are being saved to U:\krios\20190717\PilA_0063.tif 27 frames are being saved to U:\krios\20190717\PilA_0064.tif 27 frames are being saved to U:\krios\20190717\PilA_0065.tif 27 frames are being saved to U:\krios\20190717\PilA_0066.tif 27 frames are being saved to U:\krios\20190717\PilA_0067.tif Running script 17 at item 61-1 (229.83, -225.31) Remaining time to fill dewars is 18300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 22 120 120 20 Disparity in stage position after aligning to target position: 0.05 -0.47 (2nd move error -0.08 -0.26) Finished aligning to item in 1 rounds -29.1 20.3 unbinned pixels; 112.8 74.1 nm along two shift axes; -73.0 -117.5 nm on specimen axes Total_Shift = 71.00025 -2.8 13.8 unbinned pixels; 54.3 10.8 nm along two shift axes; -53.0 -13.6 nm on specimen axes Total_Shift = 28.110176 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.45 microns changed by -2.15 to target drift = 0.03 nm/sec Measured defocus = -2.58 microns changed by -0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0068.tif 27 frames are being saved to U:\krios\20190717\PilA_0069.tif 27 frames are being saved to U:\krios\20190717\PilA_0070.tif 27 frames are being saved to U:\krios\20190717\PilA_0071.tif 27 frames are being saved to U:\krios\20190717\PilA_0072.tif 27 frames are being saved to U:\krios\20190717\PilA_0073.tif Running script 17 at item 62-1 (227.27, -224.15) Remaining time to fill dewars is 18060 Dewars are NOT busy filling Trimming A by 23 120 20 180, reference by 227 20 20 240 Trimming A by 23 120 20 180, reference by 241 20 165 20 Trimming A by 23 120 20 180, reference by 22 110 20 155 Trimming A by 23 120 20 180, reference by 22 120 120 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.19 Trimming A by 20 20 20 20, reference by 22 110 20 155 Disparity in stage position after aligning to target position: 0.16 0.05 (2nd move error 0.02 0.22) Finished aligning to item in 2 rounds Trimming A by 26 20 20 20, reference by 20 20 20 20 0.9 -0.4 unbinned pixels; 2.7 2.5 nm along two shift axes; 1.4 3.5 nm on specimen axes Total_Shift = 1.935651 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.82 microns changed by -2.12 to target drift = 0.13 nm/sec Measured defocus = -1.22 microns changed by -0.08 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0074.tif 27 frames are being saved to U:\krios\20190717\PilA_0075.tif 27 frames are being saved to U:\krios\20190717\PilA_0076.tif 27 frames are being saved to U:\krios\20190717\PilA_0077.tif 27 frames are being saved to U:\krios\20190717\PilA_0078.tif 27 frames are being saved to U:\krios\20190717\PilA_0079.tif Running script 17 at item 63 (224.74, -222.51) Remaining time to fill dewars is 17940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 53 23 20 115, reference by 22 110 20 155 Disparity in stage position after aligning to target position: 0.13 -0.09 (2nd move error -0.02 0.07) Finished aligning to item in 1 rounds Trimming A by 37 22 25 115, reference by 53 23 20 115 1.4 4.8 unbinned pixels; 15.6 12.3 nm along two shift axes; -18.8 4.6 nm on specimen axes Total_Shift = 9.943554 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.68 microns changed by -2.08 to target drift = 0.13 nm/sec Measured defocus = -1.34 microns changed by -0.06 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0080.tif Ending Navigator acquisition after current item 27 frames are being saved to U:\krios\20190717\PilA_0081.tif 27 frames are being saved to U:\krios\20190717\PilA_0082.tif 27 frames are being saved to U:\krios\20190717\PilA_0083.tif 27 frames are being saved to U:\krios\20190717\PilA_0084.tif 27 frames are being saved to U:\krios\20190717\PilA_0085.tif Running script 17 at item 64 (223.15, -225.06) Remaining time to fill dewars is 17580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 34 24 20 135, reference by 22 110 20 155 Disparity in stage position after aligning to target position: 0.18 -0.19 (2nd move error 0.04 -0.02) Finished aligning to item in 1 rounds Trimming A by 34 24 20 135, reference by 34 24 20 135 -5.2 -1.2 unbinned pixels; 2.5 20.7 nm along two shift axes; 5.7 -20.2 nm on specimen axes Total_Shift = 10.766163 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.62 microns changed by -2.12 to target drift = 0.10 nm/sec Measured defocus = -1.51 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0086.tif 27 frames are being saved to U:\krios\20190717\PilA_0087.tif 27 frames are being saved to U:\krios\20190717\PilA_0088.tif 27 frames are being saved to U:\krios\20190717\PilA_0089.tif 27 frames are being saved to U:\krios\20190717\PilA_0090.tif 27 frames are being saved to U:\krios\20190717\PilA_0091.tif Running script 17 at item 65 (225.67, -226.81) Remaining time to fill dewars is 17340 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 20 41 20 85, reference by 22 110 20 155 Disparity in stage position after aligning to target position: 0.26 -0.02 (2nd move error 0.12 0.14) Finished aligning to item in 1 rounds Trimming A by 24 41 20 80, reference by 20 41 20 85 2.3 0.4 unbinned pixels; 1.4 8.8 nm along two shift axes; -2.2 8.8 nm on specimen axes Total_Shift = 4.632993 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.56 microns changed by -2.16 to target drift = 0.15 nm/sec Measured defocus = -1.64 microns changed by 0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0092.tif 27 frames are being saved to U:\krios\20190717\PilA_0093.tif 27 frames are being saved to U:\krios\20190717\PilA_0094.tif 27 frames are being saved to U:\krios\20190717\PilA_0095.tif 27 frames are being saved to U:\krios\20190717\PilA_0096.tif 27 frames are being saved to U:\krios\20190717\PilA_0097.tif Running script 17 at item 66 (228.17, -227.90) Remaining time to fill dewars is 17160 Dewars are NOT busy filling Trimming A by 23 93 140 20, reference by 227 20 20 240 Trimming A by 23 93 140 20, reference by 241 20 165 20 Trimming A by 23 93 140 20, reference by 22 110 20 155 Trimming A by 23 93 140 20, reference by 22 120 120 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.23 Trimming A by 22 45 20 60, reference by 22 120 120 20 Disparity in stage position after aligning to target position: 0.12 -0.54 (2nd move error -0.05 -0.34) Finished aligning to item in 2 rounds Trimming A by 21 50 20 60, reference by 22 45 20 60 -40.6 16.3 unbinned pixels; 113.0 121.4 nm along two shift axes; -55.0 -161.2 nm on specimen axes Total_Shift = 87.42511 Trimming A by 21 55 20 60, reference by 22 45 20 60 -5.8 9.9 unbinned pixels; 43.9 5.9 nm along two shift axes; -37.3 -24.5 nm on specimen axes Total_Shift = 22.902281 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.53 microns changed by -2.23 to target drift = 0.04 nm/sec Measured defocus = -1.73 microns changed by 0.03 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0098.tif 27 frames are being saved to U:\krios\20190717\PilA_0099.tif 27 frames are being saved to U:\krios\20190717\PilA_0100.tif 27 frames are being saved to U:\krios\20190717\PilA_0101.tif 27 frames are being saved to U:\krios\20190717\PilA_0102.tif 27 frames are being saved to U:\krios\20190717\PilA_0103.tif Running script 17 at item 67 (230.67, -229.64) Remaining time to fill dewars is 16980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 50 30, reference by 22 120 120 20 Disparity in stage position after aligning to target position: 0.20 -0.44 (2nd move error 0.02 -0.23) Finished aligning to item in 1 rounds Trimming A by 20 85 55 30, reference by 20 80 50 30 -26.9 -1.0 unbinned pixels; 31.6 98.3 nm along two shift axes; 9.4 -104.4 nm on specimen axes Total_Shift = 53.830325 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.39 microns changed by -2.19 to target drift = 0.14 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0104.tif 27 frames are being saved to U:\krios\20190717\PilA_0105.tif 27 frames are being saved to U:\krios\20190717\PilA_0106.tif 27 frames are being saved to U:\krios\20190717\PilA_0107.tif 27 frames are being saved to U:\krios\20190717\PilA_0108.tif 27 frames are being saved to U:\krios\20190717\PilA_0109.tif Running script 17 at item 68 (226.45, -231.09) Remaining time to fill dewars is 16800 Dewars are NOT busy filling Trimming A by 22 120 20 180, reference by 227 20 20 240 Trimming A by 22 120 20 180, reference by 241 20 165 20 Trimming A by 22 120 20 180, reference by 22 110 20 155 Trimming A by 22 120 20 180, reference by 22 120 120 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.21 Trimming A by 20 140 20 125, reference by 22 110 20 155 Disparity in stage position after aligning to target position: 0.27 0.05 (2nd move error 0.12 0.23) Finished aligning to item in 2 rounds Trimming A by 23 130 20 120, reference by 20 140 20 125 3.7 1.2 unbinned pixels; 0.3 15.2 nm along two shift axes; -5.5 14.1 nm on specimen axes Total_Shift = 7.790259 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.10 microns changed by -2.00 to target drift = 0.13 nm/sec Measured defocus = -1.87 microns changed by -0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0110.tif 27 frames are being saved to U:\krios\20190717\PilA_0111.tif 27 frames are being saved to U:\krios\20190717\PilA_0112.tif 27 frames are being saved to U:\krios\20190717\PilA_0113.tif 27 frames are being saved to U:\krios\20190717\PilA_0114.tif 27 frames are being saved to U:\krios\20190717\PilA_0115.tif Running script 17 at item 69 (223.94, -229.45) Remaining time to fill dewars is 16560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 115 20 145, reference by 22 110 20 155 Disparity in stage position after aligning to target position: 0.21 -0.13 (2nd move error 0.05 0.05) Finished aligning to item in 1 rounds Trimming A by 23 110 20 145, reference by 22 115 20 145 -2.9 -2.5 unbinned pixels; 5.5 14.1 nm along two shift axes; 10.4 -10.6 nm on specimen axes Total_Shift = 7.644507 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.02 microns changed by -2.02 to target drift = 0.15 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0116.tif 27 frames are being saved to U:\krios\20190717\PilA_0117.tif 27 frames are being saved to U:\krios\20190717\PilA_0118.tif 27 frames are being saved to U:\krios\20190717\PilA_0119.tif 27 frames are being saved to U:\krios\20190717\PilA_0120.tif 27 frames are being saved to U:\krios\20190717\PilA_0121.tif Running script 17 at item 70 (221.49, -227.67) Remaining time to fill dewars is 16440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 84 20 185, reference by 22 110 20 155 Disparity in stage position after aligning to target position: 0.17 -0.19 (2nd move error 0.02 -0.01) Finished aligning to item in 1 rounds Trimming A by 22 84 20 185, reference by 22 84 20 185 -2.8 -1.7 unbinned pixels; 2.6 12.5 nm along two shift axes; 7.1 -10.4 nm on specimen axes Total_Shift = 6.460022 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = -0.12 microns changed by -1.98 to target drift = 0.12 nm/sec Measured defocus = -2.12 microns changed by 0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0122.tif 27 frames are being saved to U:\krios\20190717\PilA_0123.tif 27 frames are being saved to U:\krios\20190717\PilA_0124.tif 27 frames are being saved to U:\krios\20190717\PilA_0125.tif 27 frames are being saved to U:\krios\20190717\PilA_0126.tif 27 frames are being saved to U:\krios\20190717\PilA_0127.tif Running script 17 at item 71 (266.16, -165.52) Remaining time to fill dewars is 16260 Dewars are NOT busy filling Trimming A by 255 21 180 20, reference by 235 23 20 235 Trimming A by 255 21 180 20, reference by 241 22 170 20 Trimming A by 255 21 180 20, reference by 20 145 20 175 Trimming A by 255 21 180 20, reference by 21 117 145 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.28 Trimming A by 120 20 155 20, reference by 241 22 170 20 Disparity in stage position after aligning to target position: 0.26 -0.16 (2nd move error 0.17 0.09) Finished aligning to item in 2 rounds Trimming A by 111 20 170 20, reference by 120 20 155 20 0.2 0.5 unbinned pixels; 1.7 1.5 nm along two shift axes; -2.2 0.7 nm on specimen axes Total_Shift = 1.16002 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.23 microns changed by -2.43 to target drift = 0.24 nm/sec Measured defocus = -2.14 microns changed by -0.06 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0128.tif 27 frames are being saved to U:\krios\20190717\PilA_0129.tif 27 frames are being saved to U:\krios\20190717\PilA_0130.tif 27 frames are being saved to U:\krios\20190717\PilA_0131.tif 27 frames are being saved to U:\krios\20190717\PilA_0132.tif 27 frames are being saved to U:\krios\20190717\PilA_0133.tif Running script 17 at item 72 (263.56, -163.75) Remaining time to fill dewars is 16020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 22 130 20, reference by 241 22 170 20 Disparity in stage position after aligning to target position: 0.19 -0.35 (2nd move error 0.10 -0.10) Finished aligning to item in 1 rounds Trimming A by 135 22 130 20, reference by 135 22 130 20 -11.3 -3.0 unbinned pixels; 3.9 45.1 nm along two shift axes; 13.9 -43.3 nm on specimen axes Total_Shift = 23.339977 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.12 microns changed by -2.42 to target drift = 0.17 nm/sec Measured defocus = -2.32 microns changed by 0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0134.tif 27 frames are being saved to U:\krios\20190717\PilA_0135.tif 27 frames are being saved to U:\krios\20190717\PilA_0136.tif 27 frames are being saved to U:\krios\20190717\PilA_0137.tif 27 frames are being saved to U:\krios\20190717\PilA_0138.tif 27 frames are being saved to U:\krios\20190717\PilA_0139.tif Running script 17 at item 73 (261.05, -162.10) Remaining time to fill dewars is 15840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 23 105 20, reference by 241 22 170 20 Disparity in stage position after aligning to target position: 0.21 -0.43 (2nd move error 0.12 -0.17) Finished aligning to item in 1 rounds Trimming A by 150 21 105 20, reference by 150 23 105 20 -21.3 -4.7 unbinned pixels; 10.7 83.7 nm along two shift axes; 22.6 -81.9 nm on specimen axes Total_Shift = 43.626257 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.03 microns changed by -2.43 to target drift = 0.16 nm/sec Measured defocus = -2.43 microns changed by 0.03 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0140.tif 27 frames are being saved to U:\krios\20190717\PilA_0141.tif 27 frames are being saved to U:\krios\20190717\PilA_0142.tif 27 frames are being saved to U:\krios\20190717\PilA_0143.tif 27 frames are being saved to U:\krios\20190717\PilA_0144.tif 27 frames are being saved to U:\krios\20190717\PilA_0145.tif Running script 17 at item 74 (258.53, -160.35) Remaining time to fill dewars is 15660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 180 20 80 55, reference by 241 22 170 20 Disparity in stage position after aligning to target position: 0.18 -0.54 (2nd move error 0.09 -0.29) Finished aligning to item in 1 rounds Trimming A by 180 20 70 50, reference by 180 20 80 55 -32.6 -0.8 unbinned pixels; 39.6 118.4 nm along two shift axes; 9.9 -126.6 nm on specimen axes Total_Shift = 65.1684 Trimming A by 170 20 85 55, reference by 180 20 80 55 -2.9 -0.4 unbinned pixels; 2.2 11.1 nm along two shift axes; 2.3 -11.1 nm on specimen axes Total_Shift = 5.84173 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.09 microns changed by -2.41 to target drift = 0.13 nm/sec Measured defocus = -2.57 microns changed by 0.07 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0146.tif 27 frames are being saved to U:\krios\20190717\PilA_0147.tif 27 frames are being saved to U:\krios\20190717\PilA_0148.tif 27 frames are being saved to U:\krios\20190717\PilA_0149.tif 27 frames are being saved to U:\krios\20190717\PilA_0150.tif 27 frames are being saved to U:\krios\20190717\PilA_0151.tif Running script 17 at item 75 (256.80, -163.36) Remaining time to fill dewars is 15420 Dewars are NOT busy filling Trimming A by 255 21 20 240, reference by 235 23 20 235 Trimming A by 255 21 20 240, reference by 241 22 170 20 Trimming A by 255 21 20 240, reference by 20 145 20 175 Trimming A by 255 21 20 240, reference by 21 117 145 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.21 Trimming A by 110 24 20 85, reference by 235 23 20 235 Disparity in stage position after aligning to target position: 0.42 0.02 (2nd move error 0.24 0.21) Finished aligning to item in 2 rounds Trimming A by 115 23 20 80, reference by 110 24 20 85 -0.8 4.0 unbinned pixels; 16.0 3.0 nm along two shift axes; -15.5 -4.1 nm on specimen axes Total_Shift = 8.254212 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.31 microns changed by -2.29 to target drift = 0.12 nm/sec Measured defocus = -2.61 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0152.tif 27 frames are being saved to U:\krios\20190717\PilA_0153.tif 27 frames are being saved to U:\krios\20190717\PilA_0154.tif 27 frames are being saved to U:\krios\20190717\PilA_0155.tif 27 frames are being saved to U:\krios\20190717\PilA_0156.tif 27 frames are being saved to U:\krios\20190717\PilA_0157.tif Running script 17 at item 76 (259.46, -164.63) Remaining time to fill dewars is 15300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 75 24 50 20, reference by 241 22 170 20 Disparity in stage position after aligning to target position: 0.25 -0.47 (2nd move error 0.16 -0.22) Finished aligning to item in 1 rounds Trimming A by 70 20 55 20, reference by 75 24 50 20 -24.6 -1.9 unbinned pixels; 25.2 91.5 nm along two shift axes; 12.6 -95.4 nm on specimen axes Total_Shift = 49.410665 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.05 microns changed by -2.34 to target drift = 0.17 nm/sec Measured defocus = -1.33 microns changed by 0.03 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0158.tif 27 frames are being saved to U:\krios\20190717\PilA_0159.tif 27 frames are being saved to U:\krios\20190717\PilA_0160.tif 27 frames are being saved to U:\krios\20190717\PilA_0161.tif 27 frames are being saved to U:\krios\20190717\PilA_0162.tif 27 frames are being saved to U:\krios\20190717\PilA_0163.tif Running script 17 at item 77 (262.08, -166.22) Remaining time to fill dewars is 15120 Dewars are NOT busy filling Trimming A by 22 130 135 20, reference by 235 23 20 235 Trimming A by 22 130 135 20, reference by 241 22 170 20 Trimming A by 22 130 135 20, reference by 20 145 20 175 Trimming A by 22 130 135 20, reference by 21 117 145 20 Disparity in stage position after aligning to center of map frame: 0.18 -0.29 Trimming A by 70 20 65 20, reference by 21 117 145 20 Disparity in stage position after aligning to target position: 0.06 -0.40 (2nd move error -0.11 -0.13) Finished aligning to item in 2 rounds Trimming A by 65 20 70 20, reference by 70 20 65 20 -17.8 35.5 unbinned pixels; 153.8 10.6 nm along two shift axes; -134.4 -76.7 nm on specimen axes Total_Shift = 79.419893 Trimming A by 65 20 65 20, reference by 70 20 65 20 -7.7 23.6 unbinned pixels; 96.8 7.7 nm along two shift axes; -90.1 -35.0 nm on specimen axes Total_Shift = 49.619856 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.07 microns changed by -2.47 to target drift = 0.03 nm/sec Measured defocus = -1.45 microns changed by 0.05 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0164.tif 27 frames are being saved to U:\krios\20190717\PilA_0165.tif 27 frames are being saved to U:\krios\20190717\PilA_0166.tif 27 frames are being saved to U:\krios\20190717\PilA_0167.tif 27 frames are being saved to U:\krios\20190717\PilA_0168.tif 27 frames are being saved to U:\krios\20190717\PilA_0169.tif Running script 17 at item 78 (264.66, -167.97) Remaining time to fill dewars is 14820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 32 33 120 20, reference by 21 117 145 20 Disparity in stage position after aligning to target position: 0.13 -0.27 (2nd move error -0.05 0.00) Finished aligning to item in 1 rounds Trimming A by 25 25 125 20, reference by 32 33 120 20 0.3 10.7 unbinned pixels; 39.0 17.2 nm along two shift axes; -41.7 -1.1 nm on specimen axes Total_Shift = 21.418754 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.89 microns changed by -2.39 to target drift = 0.12 nm/sec Measured defocus = -1.45 microns changed by -0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0170.tif 27 frames are being saved to U:\krios\20190717\PilA_0171.tif 27 frames are being saved to U:\krios\20190717\PilA_0172.tif 27 frames are being saved to U:\krios\20190717\PilA_0173.tif 27 frames are being saved to U:\krios\20190717\PilA_0174.tif 27 frames are being saved to U:\krios\20190717\PilA_0175.tif Running script 17 at item 79 (265.48, -172.32) Remaining time to fill dewars is 14580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 135 160 20, reference by 21 117 145 20 Disparity in stage position after aligning to target position: 0.19 -0.27 (2nd move error 0.01 0.00) Finished aligning to item in 1 rounds Trimming A by 20 135 160 20, reference by 20 135 160 20 -0.0 0.5 unbinned pixels; 2.0 0.7 nm along two shift axes; -2.1 -0.2 nm on specimen axes Total_Shift = 1.063496 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.80 microns changed by -2.40 to target drift = 0.11 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0176.tif 27 frames are being saved to U:\krios\20190717\PilA_0177.tif 27 frames are being saved to U:\krios\20190717\PilA_0178.tif 27 frames are being saved to U:\krios\20190717\PilA_0179.tif 27 frames are being saved to U:\krios\20190717\PilA_0180.tif 27 frames are being saved to U:\krios\20190717\PilA_0181.tif Running script 17 at item 80 (262.96, -170.57) Remaining time to fill dewars is 14400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 65 90 20, reference by 21 117 145 20 Disparity in stage position after aligning to target position: 0.20 -0.40 (2nd move error 0.01 -0.13) Finished aligning to item in 1 rounds Trimming A by 20 61 95 20, reference by 22 65 90 20 -17.1 0.2 unbinned pixels; 23.1 61.4 nm along two shift axes; 2.9 -66.7 nm on specimen axes Total_Shift = 34.26594 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.66 microns changed by -2.36 to target drift = 0.08 nm/sec Measured defocus = -1.65 microns changed by -0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0182.tif 27 frames are being saved to U:\krios\20190717\PilA_0183.tif 27 frames are being saved to U:\krios\20190717\PilA_0184.tif 27 frames are being saved to U:\krios\20190717\PilA_0185.tif 27 frames are being saved to U:\krios\20190717\PilA_0186.tif 27 frames are being saved to U:\krios\20190717\PilA_0187.tif Running script 17 at item 81 (260.51, -168.81) Remaining time to fill dewars is 14280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 21 117 145 20 Disparity in stage position after aligning to target position: 0.12 -0.53 (2nd move error -0.07 -0.26) Finished aligning to item in 1 rounds -32.6 13.9 unbinned pixels; 93.7 96.5 nm along two shift axes; -47.2 -129.8 nm on specimen axes Total_Shift = 70.910588 -4.1 8.2 unbinned pixels; 35.4 2.6 nm along two shift axes; -30.9 -17.8 nm on specimen axes Total_Shift = 18.301249 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.54 microns changed by -2.34 to target drift = 0.03 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0188.tif 27 frames are being saved to U:\krios\20190717\PilA_0189.tif 27 frames are being saved to U:\krios\20190717\PilA_0190.tif 27 frames are being saved to U:\krios\20190717\PilA_0191.tif 27 frames are being saved to U:\krios\20190717\PilA_0192.tif 27 frames are being saved to U:\krios\20190717\PilA_0193.tif Running script 17 at item 82 (257.98, -167.09) Remaining time to fill dewars is 14160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 31 20 20 20, reference by 21 117 145 20 Disparity in stage position after aligning to target position: 0.07 -0.61 (2nd move error -0.11 -0.34) Finished aligning to item in 1 rounds Trimming A by 21 20 20 25, reference by 31 20 20 20 -34.7 25.2 unbinned pixels; 137.8 87.1 nm along two shift axes; -90.6 -140.4 nm on specimen axes Total_Shift = 85.744481 Trimming A by 26 20 20 20, reference by 31 20 20 20 -2.3 16.5 unbinned pixels; 63.6 16.3 nm along two shift axes; -63.5 -12.6 nm on specimen axes Total_Shift = 33.253452 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.44 microns changed by -2.34 to target drift = 0.01 nm/sec Measured defocus = -1.94 microns changed by 0.04 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0194.tif 27 frames are being saved to U:\krios\20190717\PilA_0195.tif 27 frames are being saved to U:\krios\20190717\PilA_0196.tif 27 frames are being saved to U:\krios\20190717\PilA_0197.tif 27 frames are being saved to U:\krios\20190717\PilA_0198.tif 27 frames are being saved to U:\krios\20190717\PilA_0199.tif Running script 17 at item 83 (255.25, -166.02) Remaining time to fill dewars is 13920 Dewars are NOT busy filling Trimming A by 250 23 20 245, reference by 235 23 20 235 Trimming A by 250 23 20 245, reference by 241 22 170 20 Trimming A by 250 23 20 245, reference by 20 145 20 175 Trimming A by 250 23 20 245, reference by 21 117 145 20 Disparity in stage position after aligning to center of map frame: 0.19 -0.24 Trimming A by 67 24 20 110, reference by 235 23 20 235 Disparity in stage position after aligning to target position: 0.41 0.03 (2nd move error 0.21 0.23) Finished aligning to item in 2 rounds Trimming A by 61 21 20 110, reference by 67 24 20 110 2.7 -2.0 unbinned pixels; 11.0 6.6 nm along two shift axes; 7.4 10.9 nm on specimen axes Total_Shift = 6.766947 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.21 microns changed by -2.21 to target drift = 0.16 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0200.tif 27 frames are being saved to U:\krios\20190717\PilA_0201.tif 27 frames are being saved to U:\krios\20190717\PilA_0202.tif 27 frames are being saved to U:\krios\20190717\PilA_0203.tif 27 frames are being saved to U:\krios\20190717\PilA_0204.tif 27 frames are being saved to U:\krios\20190717\PilA_0205.tif Running script 17 at item 84 (253.88, -168.60) Remaining time to fill dewars is 13680 Dewars are NOT busy filling Trimming A by 21 145 20 190, reference by 235 23 20 235 Trimming A by 21 145 20 190, reference by 241 22 170 20 Trimming A by 21 145 20 190, reference by 20 145 20 175 Trimming A by 21 145 20 190, reference by 21 117 145 20 Disparity in stage position after aligning to center of map frame: 0.23 -0.31 Trimming A by 41 20 20 115, reference by 20 145 20 175 Disparity in stage position after aligning to target position: 0.20 -0.17 (2nd move error -0.03 0.12) Finished aligning to item in 2 rounds Trimming A by 37 23 20 120, reference by 41 20 20 115 1.1 8.2 unbinned pixels; 28.8 16.3 nm along two shift axes; -32.2 2.5 nm on specimen axes Total_Shift = 16.590447 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.09 microns changed by -2.19 to target drift = 0.13 nm/sec Measured defocus = -2.08 microns changed by -0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0206.tif 27 frames are being saved to U:\krios\20190717\PilA_0207.tif 27 frames are being saved to U:\krios\20190717\PilA_0208.tif 27 frames are being saved to U:\krios\20190717\PilA_0209.tif 27 frames are being saved to U:\krios\20190717\PilA_0210.tif 27 frames are being saved to U:\krios\20190717\PilA_0211.tif Running script 17 at item 85 (256.38, -170.34) Remaining time to fill dewars is 13500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 50 20 60, reference by 20 145 20 175 Disparity in stage position after aligning to target position: 0.25 -0.05 (2nd move error 0.03 0.24) Finished aligning to item in 1 rounds Trimming A by 21 45 20 55, reference by 21 50 20 60 0.6 -0.9 unbinned pixels; 4.1 0.6 nm along two shift axes; 3.5 2.3 nm on specimen axes Total_Shift = 2.143401 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.02 microns changed by -2.18 to target drift = 0.12 nm/sec Measured defocus = -2.25 microns changed by 0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0212.tif 27 frames are being saved to U:\krios\20190717\PilA_0213.tif 27 frames are being saved to U:\krios\20190717\PilA_0214.tif 27 frames are being saved to U:\krios\20190717\PilA_0215.tif 27 frames are being saved to U:\krios\20190717\PilA_0216.tif 27 frames are being saved to U:\krios\20190717\PilA_0217.tif Running script 17 at item 86 (258.89, -171.43) Remaining time to fill dewars is 13380 Dewars are NOT busy filling Trimming A by 20 112 150 20, reference by 235 23 20 235 Trimming A by 20 112 150 20, reference by 241 22 170 20 Trimming A by 20 112 150 20, reference by 20 145 20 175 Trimming A by 20 112 150 20, reference by 21 117 145 20 Disparity in stage position after aligning to center of map frame: 0.20 -0.28 Trimming A by 21 45 20 40, reference by 21 117 145 20 Disparity in stage position after aligning to target position: 0.14 -0.60 (2nd move error -0.06 -0.34) Finished aligning to item in 2 rounds Trimming A by 21 50 30 45, reference by 21 45 20 40 -29.7 12.9 unbinned pixels; 86.3 87.4 nm along two shift axes; -44.0 -118.2 nm on specimen axes Total_Shift = 64.730387 Trimming A by 21 50 20 50, reference by 21 45 20 40 -2.3 6.8 unbinned pixels; 28.0 2.1 nm along two shift axes; -26.0 -10.3 nm on specimen axes Total_Shift = 14.362275 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.05 microns changed by -2.35 to target drift = 0.04 nm/sec Measured defocus = -2.40 microns changed by 0.10 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0218.tif 27 frames are being saved to U:\krios\20190717\PilA_0219.tif 27 frames are being saved to U:\krios\20190717\PilA_0220.tif 27 frames are being saved to U:\krios\20190717\PilA_0221.tif 27 frames are being saved to U:\krios\20190717\PilA_0222.tif 27 frames are being saved to U:\krios\20190717\PilA_0223.tif Running script 17 at item 87 (261.40, -173.14) Remaining time to fill dewars is 13140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 95 70 20, reference by 21 117 145 20 Disparity in stage position after aligning to target position: 0.23 -0.48 (2nd move error 0.03 -0.22) Finished aligning to item in 1 rounds Trimming A by 21 100 70 20, reference by 22 95 70 20 -30.4 -1.7 unbinned pixels; 33.6 112.1 nm along two shift axes; 13.0 -118.1 nm on specimen axes Total_Shift = 60.986123 Trimming A by 22 100 70 20, reference by 22 95 70 20 -6.7 -1.4 unbinned pixels; 3.5 26.1 nm along two shift axes; 6.9 -25.6 nm on specimen axes Total_Shift = 13.627509 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.09 microns changed by -2.31 to target drift = 0.15 nm/sec Measured defocus = -2.43 microns changed by 0.03 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0224.tif 27 frames are being saved to U:\krios\20190717\PilA_0225.tif 27 frames are being saved to U:\krios\20190717\PilA_0226.tif 27 frames are being saved to U:\krios\20190717\PilA_0227.tif 27 frames are being saved to U:\krios\20190717\PilA_0228.tif 27 frames are being saved to U:\krios\20190717\PilA_0229.tif Running script 17 at item 88 (259.76, -175.68) Remaining time to fill dewars is 12960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 157 20 105, reference by 21 117 145 20 Disparity in stage position after aligning to target position: 0.26 -0.56 (2nd move error 0.05 -0.30) Finished aligning to item in 1 rounds Trimming A by 20 165 20 105, reference by 20 157 20 105 -28.7 -2.2 unbinned pixels; 29.5 106.4 nm along two shift axes; 14.4 -111.0 nm on specimen axes Total_Shift = 57.478176 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.29 microns changed by -2.21 to target drift = 0.22 nm/sec Measured defocus = -2.54 microns changed by 0.04 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0230.tif 27 frames are being saved to U:\krios\20190717\PilA_0231.tif 27 frames are being saved to U:\krios\20190717\PilA_0232.tif 27 frames are being saved to U:\krios\20190717\PilA_0233.tif 27 frames are being saved to U:\krios\20190717\PilA_0234.tif 27 frames are being saved to U:\krios\20190717\PilA_0235.tif Running script 17 at item 89 (257.23, -174.57) Remaining time to fill dewars is 12720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 135 20 110, reference by 20 145 20 175 Disparity in stage position after aligning to target position: 0.30 -0.02 (2nd move error 0.07 0.26) Finished aligning to item in 1 rounds Trimming A by 22 125 20 110, reference by 20 135 20 110 0.6 -0.8 unbinned pixels; 3.9 0.7 nm along two shift axes; 3.2 2.4 nm on specimen axes Total_Shift = 2.033821 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.53 microns changed by -2.07 to target drift = 0.11 nm/sec Ending Navigator acquisition after current item Measured defocus = -2.52 microns changed by -0.08 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0236.tif 27 frames are being saved to U:\krios\20190717\PilA_0237.tif 27 frames are being saved to U:\krios\20190717\PilA_0238.tif 27 frames are being saved to U:\krios\20190717\PilA_0239.tif 27 frames are being saved to U:\krios\20190717\PilA_0240.tif 27 frames are being saved to U:\krios\20190717\PilA_0241.tif Running script 17 at item 90 (254.74, -172.92) Remaining time to fill dewars is 12540 Dewars are NOT busy filling Trimming A by 23 132 20 200, reference by 235 23 20 235 Trimming A by 23 132 20 200, reference by 241 22 170 20 Trimming A by 23 132 20 200, reference by 20 145 20 175 Trimming A by 23 132 20 200, reference by 21 117 145 20 Disparity in stage position after aligning to center of map frame: 0.20 -0.28 Trimming A by 24 115 20 125, reference by 20 145 20 175 Disparity in stage position after aligning to target position: 0.26 -0.11 (2nd move error 0.06 0.15) Finished aligning to item in 2 rounds Trimming A by 24 110 20 125, reference by 24 115 20 125 -2.8 -1.0 unbinned pixels; 0.0 11.6 nm along two shift axes; 4.5 -10.7 nm on specimen axes Total_Shift = 5.952736 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.92 microns changed by -2.22 to target drift = 0.12 nm/sec Measured defocus = -1.30 microns changed by 0.00 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0242.tif 27 frames are being saved to U:\krios\20190717\PilA_0243.tif 27 frames are being saved to U:\krios\20190717\PilA_0244.tif 27 frames are being saved to U:\krios\20190717\PilA_0245.tif 27 frames are being saved to U:\krios\20190717\PilA_0246.tif 27 frames are being saved to U:\krios\20190717\PilA_0247.tif Running script 17 at item 91 (252.21, -171.20) Remaining time to fill dewars is 12240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 95 20 155, reference by 20 145 20 175 Disparity in stage position after aligning to target position: 0.24 -0.25 (2nd move error 0.03 -0.00) Finished aligning to item in 1 rounds Trimming A by 21 95 20 155, reference by 21 95 20 155 -2.3 -2.9 unbinned pixels; 7.8 12.6 nm along two shift axes; 11.9 -8.2 nm on specimen axes Total_Shift = 7.412632 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.84 microns changed by -2.24 to target drift = 0.14 nm/sec Measured defocus = -1.46 microns changed by 0.06 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0248.tif 27 frames are being saved to U:\krios\20190717\PilA_0249.tif 27 frames are being saved to U:\krios\20190717\PilA_0250.tif 27 frames are being saved to U:\krios\20190717\PilA_0251.tif 27 frames are being saved to U:\krios\20190717\PilA_0252.tif 27 frames are being saved to U:\krios\20190717\PilA_0253.tif Running script 17 at item 92 (317.91, -194.89) Remaining time to fill dewars is 12180 Dewars are NOT busy filling Trimming A by 230 21 175 20, reference by 221 22 20 240 Trimming A by 230 21 175 20, reference by 215 21 170 20 Trimming A by 230 21 175 20, reference by 21 190 20 220 Trimming A by 230 21 175 20, reference by 22 170 160 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.31 Trimming A by 140 23 150 20, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.19 -0.34 (2nd move error 0.13 -0.05) Finished aligning to item in 2 rounds Trimming A by 140 24 155 20, reference by 140 23 150 20 -4.9 -2.4 unbinned pixels; 2.6 21.3 nm along two shift axes; 10.5 -18.6 nm on specimen axes Total_Shift = 10.954486 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.05 microns changed by -2.55 to target drift = 0.18 nm/sec Measured defocus = -1.50 microns changed by -0.00 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0254.tif 27 frames are being saved to U:\krios\20190717\PilA_0255.tif 27 frames are being saved to U:\krios\20190717\PilA_0256.tif 27 frames are being saved to U:\krios\20190717\PilA_0257.tif 27 frames are being saved to U:\krios\20190717\PilA_0258.tif 27 frames are being saved to U:\krios\20190717\PilA_0259.tif Running script 17 at item 93 (315.38, -193.26) Remaining time to fill dewars is 11940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 155 24 120 20, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.17 -0.42 (2nd move error 0.10 -0.13) Finished aligning to item in 1 rounds Trimming A by 155 22 120 20, reference by 155 24 120 20 -16.0 -1.6 unbinned pixels; 15.2 59.9 nm along two shift axes; 9.4 -61.9 nm on specimen axes Total_Shift = 32.142395 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.96 microns changed by -2.56 to target drift = 0.12 nm/sec Measured defocus = -1.67 microns changed by 0.07 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0260.tif 27 frames are being saved to U:\krios\20190717\PilA_0261.tif 27 frames are being saved to U:\krios\20190717\PilA_0262.tif 27 frames are being saved to U:\krios\20190717\PilA_0263.tif 27 frames are being saved to U:\krios\20190717\PilA_0264.tif 27 frames are being saved to U:\krios\20190717\PilA_0265.tif Running script 17 at item 94 (312.89, -191.54) Remaining time to fill dewars is 11760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 180 20 95 40, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.11 -0.53 (2nd move error 0.05 -0.24) Finished aligning to item in 1 rounds Trimming A by 180 20 85 35, reference by 180 20 95 40 -30.6 -0.6 unbinned pixels; 37.7 111.0 nm along two shift axes; 8.8 -118.9 nm on specimen axes Total_Shift = 61.200429 Trimming A by 180 20 80 30, reference by 180 20 95 40 -5.1 0.2 unbinned pixels; 7.5 18.0 nm along two shift axes; 0.2 -19.8 nm on specimen axes Total_Shift = 10.183644 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.77 microns changed by -2.47 to target drift = 0.11 nm/sec Measured defocus = -1.70 microns changed by 0.00 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0266.tif 27 frames are being saved to U:\krios\20190717\PilA_0267.tif 27 frames are being saved to U:\krios\20190717\PilA_0268.tif 27 frames are being saved to U:\krios\20190717\PilA_0269.tif 27 frames are being saved to U:\krios\20190717\PilA_0270.tif 27 frames are being saved to U:\krios\20190717\PilA_0271.tif Running script 17 at item 95 (308.67, -192.96) Remaining time to fill dewars is 11580 Dewars are NOT busy filling Trimming A by 232 21 20 255, reference by 221 22 20 240 Trimming A by 232 21 20 255, reference by 215 21 170 20 Trimming A by 232 21 20 255, reference by 21 190 20 220 Trimming A by 232 21 20 255, reference by 22 170 160 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.25 Trimming A by 170 20 20 140, reference by 221 22 20 240 Disparity in stage position after aligning to target position: 0.29 -0.10 (2nd move error 0.10 0.12) Finished aligning to item in 2 rounds Trimming A by 170 21 20 140, reference by 170 20 20 140 -0.0 -1.7 unbinned pixels; 6.2 2.7 nm along two shift axes; 6.6 0.2 nm on specimen axes Total_Shift = 3.371068 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.57 microns changed by -2.37 to target drift = 0.15 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0272.tif 27 frames are being saved to U:\krios\20190717\PilA_0273.tif 27 frames are being saved to U:\krios\20190717\PilA_0274.tif 27 frames are being saved to U:\krios\20190717\PilA_0275.tif 27 frames are being saved to U:\krios\20190717\PilA_0276.tif 27 frames are being saved to U:\krios\20190717\PilA_0277.tif Running script 17 at item 96 (311.27, -194.11) Remaining time to fill dewars is 11340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 23 20 80, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.15 -0.56 (2nd move error 0.09 -0.27) Finished aligning to item in 1 rounds Trimming A by 110 20 20 75, reference by 115 23 20 80 -29.9 -2.8 unbinned pixels; 28.6 112.0 nm along two shift axes; 17.3 -115.9 nm on specimen axes Total_Shift = 60.140392 Trimming A by 105 24 20 80, reference by 115 23 20 80 -5.0 -1.9 unbinned pixels; 0.3 21.0 nm along two shift axes; 8.4 -19.2 nm on specimen axes Total_Shift = 10.770298 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.55 microns changed by -2.45 to target drift = 0.16 nm/sec Measured defocus = -1.95 microns changed by 0.05 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0278.tif 27 frames are being saved to U:\krios\20190717\PilA_0279.tif 27 frames are being saved to U:\krios\20190717\PilA_0280.tif 27 frames are being saved to U:\krios\20190717\PilA_0281.tif 27 frames are being saved to U:\krios\20190717\PilA_0282.tif 27 frames are being saved to U:\krios\20190717\PilA_0283.tif Running script 17 at item 97 (313.84, -195.83) Remaining time to fill dewars is 11280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 80 24 55 20, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.18 -0.43 (2nd move error 0.13 -0.14) Finished aligning to item in 1 rounds Trimming A by 80 23 60 20, reference by 80 24 55 20 -15.7 -0.9 unbinned pixels; 17.1 58.0 nm along two shift axes; 7.0 -61.0 nm on specimen axes Total_Shift = 31.508412 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.42 microns changed by -2.42 to target drift = 0.14 nm/sec Measured defocus = -2.02 microns changed by 0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0284.tif 27 frames are being saved to U:\krios\20190717\PilA_0285.tif 27 frames are being saved to U:\krios\20190717\PilA_0286.tif 27 frames are being saved to U:\krios\20190717\PilA_0287.tif 27 frames are being saved to U:\krios\20190717\PilA_0288.tif 27 frames are being saved to U:\krios\20190717\PilA_0289.tif Running script 17 at item 98 (316.28, -197.41) Remaining time to fill dewars is 11100 Dewars are NOT busy filling Trimming A by 224 22 180 20, reference by 221 22 20 240 Trimming A by 224 22 180 20, reference by 215 21 170 20 Trimming A by 224 22 180 20, reference by 21 190 20 220 Trimming A by 224 22 180 20, reference by 22 170 160 20 Disparity in stage position after aligning to center of map frame: 0.11 -0.28 Trimming A by 70 20 85 20, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.24 -0.38 (2nd move error 0.12 -0.12) Finished aligning to item in 2 rounds Trimming A by 70 20 85 20, reference by 70 20 85 20 -10.7 1.9 unbinned pixels; 20.9 35.6 nm along two shift axes; -5.1 -41.9 nm on specimen axes Total_Shift = 21.678312 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.43 microns changed by -2.53 to target drift = 0.15 nm/sec Measured defocus = -2.18 microns changed by 0.08 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0290.tif 27 frames are being saved to U:\krios\20190717\PilA_0291.tif 27 frames are being saved to U:\krios\20190717\PilA_0292.tif 27 frames are being saved to U:\krios\20190717\PilA_0293.tif 27 frames are being saved to U:\krios\20190717\PilA_0294.tif 27 frames are being saved to U:\krios\20190717\PilA_0295.tif Running script 17 at item 99 (319.09, -199.18) Remaining time to fill dewars is 10860 Dewars are NOT busy filling Trimming A by 22 170 160 20, reference by 221 22 20 240 Trimming A by 22 170 160 20, reference by 215 21 170 20 Trimming A by 22 170 160 20, reference by 21 190 20 220 Trimming A by 22 170 160 20, reference by 22 170 160 20 Disparity in stage position after aligning to center of map frame: 0.11 -0.37 Trimming A by 22 66 160 20, reference by 22 170 160 20 Disparity in stage position after aligning to target position: 0.01 -0.35 (2nd move error -0.12 -0.00) Finished aligning to item in 2 rounds Trimming A by 23 75 155 20, reference by 22 66 160 20 0.5 21.9 unbinned pixels; 79.8 34.9 nm along two shift axes; -85.3 -2.5 nm on specimen axes Total_Shift = 43.811913 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.36 microns changed by -2.56 to target drift = 0.12 nm/sec Measured defocus = -2.20 microns changed by 0.00 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0296.tif 27 frames are being saved to U:\krios\20190717\PilA_0297.tif 27 frames are being saved to U:\krios\20190717\PilA_0298.tif 27 frames are being saved to U:\krios\20190717\PilA_0299.tif 27 frames are being saved to U:\krios\20190717\PilA_0300.tif 27 frames are being saved to U:\krios\20190717\PilA_0301.tif Running script 17 at item 100 (317.41, -201.78) Remaining time to fill dewars is 10620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 110 125 20, reference by 22 170 160 20 Disparity in stage position after aligning to target position: 0.06 -0.42 (2nd move error -0.06 -0.08) Finished aligning to item in 1 rounds Trimming A by 20 101 135 20, reference by 24 110 125 20 -10.0 13.4 unbinned pixels; 62.5 15.7 nm along two shift axes; -50.2 -41.6 nm on specimen axes Total_Shift = 33.483109 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.15 microns changed by -2.45 to target drift = 0.05 nm/sec Measured defocus = -2.28 microns changed by -0.02 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0302.tif 27 frames are being saved to U:\krios\20190717\PilA_0303.tif 27 frames are being saved to U:\krios\20190717\PilA_0304.tif 27 frames are being saved to U:\krios\20190717\PilA_0305.tif 27 frames are being saved to U:\krios\20190717\PilA_0306.tif 27 frames are being saved to U:\krios\20190717\PilA_0307.tif Running script 17 at item 101 (314.82, -199.93) Remaining time to fill dewars is 10440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 40 50 20, reference by 22 170 160 20 Disparity in stage position after aligning to target position: 0.04 -0.54 (2nd move error -0.08 -0.19) Finished aligning to item in 1 rounds Trimming A by 21 45 50 20, reference by 21 40 50 20 -25.3 18.8 unbinned pixels; 102.2 62.8 nm along two shift axes; -67.8 -102.4 nm on specimen axes Total_Shift = 63.059985 Trimming A by 21 45 50 20, reference by 21 40 50 20 -7.7 11.9 unbinned pixels; 53.7 10.0 nm along two shift axes; -44.5 -32.6 nm on specimen axes Total_Shift = 28.333509 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.05 microns changed by -2.45 to target drift = 0.02 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0308.tif 27 frames are being saved to U:\krios\20190717\PilA_0309.tif 27 frames are being saved to U:\krios\20190717\PilA_0310.tif 27 frames are being saved to U:\krios\20190717\PilA_0311.tif 27 frames are being saved to U:\krios\20190717\PilA_0312.tif 27 frames are being saved to U:\krios\20190717\PilA_0313.tif Running script 17 at item 102 (312.08, -198.19) Remaining time to fill dewars is 10320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 31 20 20 20, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.25 -0.59 (2nd move error 0.13 -0.33) Finished aligning to item in 1 rounds Trimming A by 20 20 20 20, reference by 31 20 20 20 -29.1 -2.4 unbinned pixels; 29.2 108.5 nm along two shift axes; 15.5 -112.9 nm on specimen axes Total_Shift = 58.494679 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.10 microns changed by -2.40 to target drift = 0.19 nm/sec Measured defocus = -2.54 microns changed by 0.04 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0314.tif 27 frames are being saved to U:\krios\20190717\PilA_0315.tif 27 frames are being saved to U:\krios\20190717\PilA_0316.tif 27 frames are being saved to U:\krios\20190717\PilA_0317.tif 27 frames are being saved to U:\krios\20190717\PilA_0318.tif 27 frames are being saved to U:\krios\20190717\PilA_0319.tif Running script 17 at item 103 (309.53, -197.23) Remaining time to fill dewars is 10020 Dewars are NOT busy filling Trimming A by 228 21 20 260, reference by 221 22 20 240 Trimming A by 228 21 20 260, reference by 215 21 170 20 Trimming A by 228 21 20 260, reference by 21 190 20 220 Trimming A by 228 21 20 260, reference by 22 170 160 20 Disparity in stage position after aligning to center of map frame: 0.18 -0.26 Trimming A by 53 23 20 110, reference by 221 22 20 240 Disparity in stage position after aligning to target position: 0.50 -0.16 (2nd move error 0.31 0.07) Finished aligning to item in 2 rounds Trimming A by 60 20 20 95, reference by 53 23 20 110 1.1 2.0 unbinned pixels; 5.8 6.9 nm along two shift axes; -7.9 3.9 nm on specimen axes Total_Shift = 4.49115 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.29 microns changed by -2.31 to target drift = 0.14 nm/sec Measured defocus = -2.55 microns changed by -0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0320.tif 27 frames are being saved to U:\krios\20190717\PilA_0321.tif 27 frames are being saved to U:\krios\20190717\PilA_0322.tif 27 frames are being saved to U:\krios\20190717\PilA_0323.tif 27 frames are being saved to U:\krios\20190717\PilA_0324.tif 27 frames are being saved to U:\krios\20190717\PilA_0325.tif Running script 17 at item 104 (307.08, -195.52) Remaining time to fill dewars is 9840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 131 20 20 170, reference by 221 22 20 240 Disparity in stage position after aligning to target position: 0.35 -0.25 (2nd move error 0.16 -0.01) Finished aligning to item in 1 rounds Trimming A by 121 21 20 170, reference by 131 20 20 170 -0.8 4.3 unbinned pixels; 16.8 3.4 nm along two shift axes; -16.4 -4.2 nm on specimen axes Total_Shift = 8.704104 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = -0.28 microns changed by -1.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0326.tif 27 frames are being saved to U:\krios\20190717\PilA_0327.tif 27 frames are being saved to U:\krios\20190717\PilA_0328.tif 27 frames are being saved to U:\krios\20190717\PilA_0329.tif 27 frames are being saved to U:\krios\20190717\PilA_0330.tif 27 frames are being saved to U:\krios\20190717\PilA_0331.tif Running script 17 at item 105 (308.15, -199.77) Remaining time to fill dewars is 9660 Dewars are NOT busy filling Trimming A by 23 190 20 230, reference by 221 22 20 240 Trimming A by 23 190 20 230, reference by 215 21 170 20 Trimming A by 23 190 20 230, reference by 21 190 20 220 Trimming A by 23 190 20 230, reference by 22 170 160 20 Disparity in stage position after aligning to center of map frame: 0.21 -0.32 Trimming A by 55 55 20 100, reference by 21 190 20 220 Disparity in stage position after aligning to target position: 0.18 -0.17 (2nd move error -0.04 0.13) Finished aligning to item in 2 rounds Trimming A by 45 45 20 105, reference by 55 55 20 100 -2.6 8.6 unbinned pixels; 35.0 3.8 nm along two shift axes; -33.0 -11.8 nm on specimen axes Total_Shift = 17.972012 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.00 microns changed by -2.40 to target drift = 0.11 nm/sec Measured defocus = -1.36 microns changed by -0.04 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0332.tif 27 frames are being saved to U:\krios\20190717\PilA_0333.tif 27 frames are being saved to U:\krios\20190717\PilA_0334.tif 27 frames are being saved to U:\krios\20190717\PilA_0335.tif 27 frames are being saved to U:\krios\20190717\PilA_0336.tif 27 frames are being saved to U:\krios\20190717\PilA_0337.tif Running script 17 at item 106 (310.67, -201.40) Remaining time to fill dewars is 9480 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 21 65 20 80, reference by 21 190 20 220 Disparity in stage position after aligning to target position: 0.22 -0.09 (2nd move error 0.01 0.20) Finished aligning to item in 1 rounds Trimming A by 21 60 20 75, reference by 21 65 20 80 1.1 0.2 unbinned pixels; 0.6 4.3 nm along two shift axes; -1.1 4.2 nm on specimen axes Total_Shift = 2.240284 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.88 microns changed by -2.38 to target drift = 0.13 nm/sec Measured defocus = -1.45 microns changed by -0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0338.tif 27 frames are being saved to U:\krios\20190717\PilA_0339.tif 27 frames are being saved to U:\krios\20190717\PilA_0340.tif 27 frames are being saved to U:\krios\20190717\PilA_0341.tif 27 frames are being saved to U:\krios\20190717\PilA_0342.tif 27 frames are being saved to U:\krios\20190717\PilA_0343.tif Running script 17 at item 107 (313.23, -202.61) Remaining time to fill dewars is 9360 Dewars are NOT busy filling Trimming A by 20 175 160 20, reference by 221 22 20 240 Trimming A by 20 175 160 20, reference by 215 21 170 20 Trimming A by 20 175 160 20, reference by 21 190 20 220 Trimming A by 20 175 160 20, reference by 22 170 160 20 Disparity in stage position after aligning to center of map frame: 0.11 -0.30 Trimming A by 21 75 45 50, reference by 22 170 160 20 Disparity in stage position after aligning to target position: 0.04 -0.62 (2nd move error -0.08 -0.35) Finished aligning to item in 2 rounds Trimming A by 21 80 50 55, reference by 21 75 45 50 -31.8 17.2 unbinned pixels; 104.9 88.5 nm along two shift axes; -60.4 -127.3 nm on specimen axes Total_Shift = 72.354445 Trimming A by 20 80 35 60, reference by 21 75 45 50 -3.2 9.7 unbinned pixels; 40.0 2.9 nm along two shift axes; -37.1 -14.7 nm on specimen axes Total_Shift = 20.483174 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.96 microns changed by -2.56 to target drift = 0.04 nm/sec Measured defocus = -1.66 microns changed by 0.06 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0344.tif 27 frames are being saved to U:\krios\20190717\PilA_0345.tif 27 frames are being saved to U:\krios\20190717\PilA_0346.tif 27 frames are being saved to U:\krios\20190717\PilA_0347.tif 27 frames are being saved to U:\krios\20190717\PilA_0348.tif 27 frames are being saved to U:\krios\20190717\PilA_0349.tif Running script 17 at item 108 (315.82, -204.37) Remaining time to fill dewars is 9180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 140 100 20, reference by 22 170 160 20 Disparity in stage position after aligning to target position: 0.16 -0.45 (2nd move error 0.04 -0.17) Finished aligning to item in 1 rounds Trimming A by 21 140 105 20, reference by 20 140 100 20 -22.2 0.0 unbinned pixels; 29.1 79.8 nm along two shift axes; 4.5 -86.2 nm on specimen axes Total_Shift = 44.336353 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.70 microns changed by -2.40 to target drift = 0.15 nm/sec Measured defocus = -1.66 microns changed by -0.04 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0350.tif 27 frames are being saved to U:\krios\20190717\PilA_0351.tif 27 frames are being saved to U:\krios\20190717\PilA_0352.tif 27 frames are being saved to U:\krios\20190717\PilA_0353.tif 27 frames are being saved to U:\krios\20190717\PilA_0354.tif 27 frames are being saved to U:\krios\20190717\PilA_0355.tif Running script 17 at item 109 (311.54, -205.74) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 160 20 120, reference by 21 190 20 220 Disparity in stage position after aligning to target position: 0.32 -0.10 (2nd move error 0.10 0.19) Finished aligning to item in 1 rounds Trimming A by 24 155 20 115, reference by 24 160 20 120 1.4 -1.6 unbinned pixels; 7.6 2.8 nm along two shift axes; 5.8 5.9 nm on specimen axes Total_Shift = 4.240389 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.56 microns changed by -2.36 to target drift = 0.13 nm/sec Measured defocus = -1.76 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0356.tif 27 frames are being saved to U:\krios\20190717\PilA_0357.tif 27 frames are being saved to U:\krios\20190717\PilA_0358.tif 27 frames are being saved to U:\krios\20190717\PilA_0359.tif 27 frames are being saved to U:\krios\20190717\PilA_0360.tif 27 frames are being saved to U:\krios\20190717\PilA_0361.tif Running script 17 at item 110 (309.01, -204.05) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 140 20 140, reference by 21 190 20 220 Disparity in stage position after aligning to target position: 0.29 -0.19 (2nd move error 0.08 0.10) Finished aligning to item in 1 rounds Trimming A by 21 140 60 135, reference by 24 140 20 140 -0.3 -1.3 unbinned pixels; 4.3 2.9 nm along two shift axes; 5.0 -0.8 nm on specimen axes Total_Shift = 2.614475 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.38 microns changed by -2.28 to target drift = 0.14 nm/sec Measured defocus = -1.84 microns changed by -0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0362.tif 27 frames are being saved to U:\krios\20190717\PilA_0363.tif 27 frames are being saved to U:\krios\20190717\PilA_0364.tif 27 frames are being saved to U:\krios\20190717\PilA_0365.tif 27 frames are being saved to U:\krios\20190717\PilA_0366.tif 27 frames are being saved to U:\krios\20190717\PilA_0367.tif Running script 17 at item 111 (289.97, -252.91) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Trimming A by 249 22 190 20, reference by 230 21 20 210 Trimming A by 249 22 190 20, reference by 229 20 185 20 Trimming A by 249 22 190 20, reference by 21 165 55 170 Trimming A by 249 22 190 20, reference by 20 160 150 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.34 Trimming A by 95 36 145 20, reference by 229 20 185 20 Disparity in stage position after aligning to target position: 0.19 -0.23 (2nd move error 0.14 0.08) Finished aligning to item in 2 rounds Trimming A by 95 35 150 20, reference by 95 36 145 20 0.5 0.9 unbinned pixels; 2.5 3.2 nm along two shift axes; -3.5 1.8 nm on specimen axes Total_Shift = 2.024309 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.24 microns changed by -2.24 to target drift = 0.17 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0368.tif 27 frames are being saved to U:\krios\20190717\PilA_0369.tif 27 frames are being saved to U:\krios\20190717\PilA_0370.tif 27 frames are being saved to U:\krios\20190717\PilA_0371.tif 27 frames are being saved to U:\krios\20190717\PilA_0372.tif 27 frames are being saved to U:\krios\20190717\PilA_0373.tif Running script 17 at item 112 (287.25, -251.36) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 105 24 110 20, reference by 229 20 185 20 Disparity in stage position after aligning to target position: 0.19 -0.38 (2nd move error 0.14 -0.06) Finished aligning to item in 1 rounds Trimming A by 105 24 115 20, reference by 105 24 110 20 -5.2 -1.1 unbinned pixels; 2.8 20.5 nm along two shift axes; 5.4 -20.1 nm on specimen axes Total_Shift = 10.693035 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.07 microns changed by -2.17 to target drift = 0.10 nm/sec Measured defocus = -2.18 microns changed by 0.08 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0374.tif 27 frames are being saved to U:\krios\20190717\PilA_0375.tif 27 frames are being saved to U:\krios\20190717\PilA_0376.tif 27 frames are being saved to U:\krios\20190717\PilA_0377.tif 27 frames are being saved to U:\krios\20190717\PilA_0378.tif 27 frames are being saved to U:\krios\20190717\PilA_0379.tif Running script 17 at item 113 (284.73, -249.71) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 22 90 20, reference by 229 20 185 20 Disparity in stage position after aligning to target position: 0.16 -0.50 (2nd move error 0.10 -0.17) Finished aligning to item in 1 rounds Trimming A by 115 22 90 20, reference by 115 22 90 20 -20.7 -5.2 unbinned pixels; 7.8 82.2 nm along two shift axes; 24.6 -79.3 nm on specimen axes Total_Shift = 42.611382 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.11 microns changed by -2.09 to target drift = 0.23 nm/sec Measured defocus = -2.25 microns changed by 0.05 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0380.tif 27 frames are being saved to U:\krios\20190717\PilA_0381.tif 27 frames are being saved to U:\krios\20190717\PilA_0382.tif 27 frames are being saved to U:\krios\20190717\PilA_0383.tif 27 frames are being saved to U:\krios\20190717\PilA_0384.tif 27 frames are being saved to U:\krios\20190717\PilA_0385.tif Running script 17 at item 114 (282.25, -247.99) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 20 55 50, reference by 229 20 185 20 Disparity in stage position after aligning to target position: 0.11 -0.57 (2nd move error 0.06 -0.25) Finished aligning to item in 1 rounds Trimming A by 130 20 75 55, reference by 140 20 55 50 -32.1 -1.9 unbinned pixels; 35.1 118.4 nm along two shift axes; 14.0 -124.5 nm on specimen axes Total_Shift = 64.331549 Trimming A by 130 20 65 55, reference by 140 20 55 50 -6.2 -1.0 unbinned pixels; 4.4 23.9 nm along two shift axes; 5.3 -23.9 nm on specimen axes Total_Shift = 12.580619 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.35 microns changed by -1.95 to target drift = 0.19 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0386.tif 27 frames are being saved to U:\krios\20190717\PilA_0387.tif 27 frames are being saved to U:\krios\20190717\PilA_0388.tif 27 frames are being saved to U:\krios\20190717\PilA_0389.tif 27 frames are being saved to U:\krios\20190717\PilA_0390.tif 27 frames are being saved to U:\krios\20190717\PilA_0391.tif Running script 17 at item 115 (279.61, -246.87) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Trimming A by 231 21 20 235, reference by 230 21 20 210 Trimming A by 231 21 20 235, reference by 229 20 185 20 Trimming A by 231 21 20 235, reference by 21 165 55 170 Trimming A by 231 21 20 235, reference by 20 160 150 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.25 Trimming A by 190 20 20 125, reference by 230 21 20 210 Disparity in stage position after aligning to target position: 0.28 -0.03 (2nd move error 0.14 0.20) Finished aligning to item in 2 rounds Trimming A by 194 24 20 125, reference by 190 20 20 125 -5.4 -0.7 unbinned pixels; 4.4 20.4 nm along two shift axes; 3.9 -20.8 nm on specimen axes Total_Shift = 10.849159 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.58 microns changed by -1.82 to target drift = 0.12 nm/sec Measured defocus = -2.35 microns changed by -0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0392.tif 27 frames are being saved to U:\krios\20190717\PilA_0393.tif 27 frames are being saved to U:\krios\20190717\PilA_0394.tif 27 frames are being saved to U:\krios\20190717\PilA_0395.tif 27 frames are being saved to U:\krios\20190717\PilA_0396.tif 27 frames are being saved to U:\krios\20190717\PilA_0397.tif Running script 17 at item 116 (277.95, -249.51) Remaining time to fill dewars is 8880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 20 20 150, reference by 230 21 20 210 Disparity in stage position after aligning to target position: 0.35 -0.16 (2nd move error 0.21 0.06) Finished aligning to item in 1 rounds Trimming A by 141 24 20 150, reference by 145 20 20 150 2.6 4.3 unbinned pixels; 12.5 15.7 nm along two shift axes; -17.3 9.1 nm on specimen axes Total_Shift = 10.016855 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.75 microns changed by -1.75 to target drift = 0.10 nm/sec Measured defocus = -2.54 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0398.tif 27 frames are being saved to U:\krios\20190717\PilA_0399.tif Ending Navigator acquisition after current item 27 frames are being saved to U:\krios\20190717\PilA_0400.tif 27 frames are being saved to U:\krios\20190717\PilA_0401.tif 27 frames are being saved to U:\krios\20190717\PilA_0402.tif 27 frames are being saved to U:\krios\20190717\PilA_0403.tif Running script 17 at item 117 (280.47, -251.07) Remaining time to fill dewars is 8820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 24 20 80, reference by 230 21 20 210 Disparity in stage position after aligning to target position: 0.48 -0.07 (2nd move error 0.34 0.16) Finished aligning to item in 1 rounds Trimming A by 90 24 20 70, reference by 90 24 20 80 2.6 7.0 unbinned pixels; 22.3 19.7 nm along two shift axes; -27.7 8.5 nm on specimen axes Total_Shift = 14.851546 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.83 microns changed by -1.77 to target drift = 0.12 nm/sec Measured defocus = -2.56 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0404.tif 27 frames are being saved to U:\krios\20190717\PilA_0405.tif 27 frames are being saved to U:\krios\20190717\PilA_0406.tif 27 frames are being saved to U:\krios\20190717\PilA_0407.tif 27 frames are being saved to U:\krios\20190717\PilA_0408.tif 27 frames are being saved to U:\krios\20190717\PilA_0409.tif Running script 17 at item 118 (283.21, -252.16) Remaining time to fill dewars is 7680 Dewars are NOT busy filling Trimming A by 250 24 180 20, reference by 230 21 20 210 Trimming A by 250 24 180 20, reference by 229 20 185 20 Trimming A by 250 24 180 20, reference by 21 165 55 170 Trimming A by 250 24 180 20, reference by 20 160 150 20 Disparity in stage position after aligning to center of map frame: 0.11 -0.25 Trimming A by 50 20 35 20, reference by 229 20 185 20 Disparity in stage position after aligning to target position: 0.15 -0.53 (2nd move error 0.04 -0.30) Finished aligning to item in 2 rounds Trimming A by 45 20 35 20, reference by 50 20 35 20 -31.2 -3.0 unbinned pixels; 29.6 116.9 nm along two shift axes; 18.3 -120.8 nm on specimen axes Total_Shift = 62.701719 Trimming A by 40 20 35 20, reference by 50 20 35 20 -4.4 -1.8 unbinned pixels; 1.0 18.6 nm along two shift axes; 8.1 -16.7 nm on specimen axes Total_Shift = 9.54822 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.70 microns changed by -2.00 to target drift = 0.17 nm/sec Measured defocus = -1.29 microns changed by -0.01 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0410.tif 27 frames are being saved to U:\krios\20190717\PilA_0411.tif 27 frames are being saved to U:\krios\20190717\PilA_0412.tif 27 frames are being saved to U:\krios\20190717\PilA_0413.tif 27 frames are being saved to U:\krios\20190717\PilA_0414.tif 27 frames are being saved to U:\krios\20190717\PilA_0415.tif Running script 17 at item 119 (285.73, -253.83) Remaining time to fill dewars is 7500 Dewars are NOT busy filling Trimming A by 22 155 155 20, reference by 230 21 20 210 Trimming A by 22 155 155 20, reference by 229 20 185 20 Trimming A by 22 155 155 20, reference by 21 165 55 170 Trimming A by 22 155 155 20, reference by 20 160 150 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.34 Trimming A by 40 21 50 20, reference by 20 160 150 20 Disparity in stage position after aligning to target position: 0.05 -0.57 (2nd move error -0.10 -0.25) Finished aligning to item in 2 rounds Trimming A by 40 21 45 20, reference by 40 21 50 20 -30.3 19.8 unbinned pixels; 112.3 79.4 nm along two shift axes; -70.6 -122.2 nm on specimen axes Total_Shift = 72.423805 Trimming A by 35 21 45 20, reference by 40 21 50 20 -3.1 11.5 unbinned pixels; 46.2 5.9 nm along two shift axes; -43.9 -14.6 nm on specimen axes Total_Shift = 23.757824 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.73 microns changed by -2.13 to target drift = 0.02 nm/sec Measured defocus = -1.43 microns changed by 0.03 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0416.tif 27 frames are being saved to U:\krios\20190717\PilA_0417.tif 27 frames are being saved to U:\krios\20190717\PilA_0418.tif 27 frames are being saved to U:\krios\20190717\PilA_0419.tif 27 frames are being saved to U:\krios\20190717\PilA_0420.tif 27 frames are being saved to U:\krios\20190717\PilA_0421.tif Running script 17 at item 120 (288.21, -255.62) Remaining time to fill dewars is 7320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 41 135 20, reference by 20 160 150 20 Disparity in stage position after aligning to target position: 0.18 -0.32 (2nd move error 0.03 -0.00) Finished aligning to item in 1 rounds Trimming A by 20 48 130 20, reference by 21 41 135 20 -2.7 -1.4 unbinned pixels; 1.8 11.8 nm along two shift axes; 6.2 -10.1 nm on specimen axes Total_Shift = 6.066126 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.66 microns changed by -2.16 to target drift = 0.13 nm/sec Measured defocus = -1.53 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0422.tif 27 frames are being saved to U:\krios\20190717\PilA_0423.tif 27 frames are being saved to U:\krios\20190717\PilA_0424.tif 27 frames are being saved to U:\krios\20190717\PilA_0425.tif 27 frames are being saved to U:\krios\20190717\PilA_0426.tif 27 frames are being saved to U:\krios\20190717\PilA_0427.tif Running script 17 at item 121 (286.61, -258.21) Remaining time to fill dewars is 7080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 100 90 20, reference by 20 160 150 20 Disparity in stage position after aligning to target position: 0.22 -0.50 (2nd move error 0.07 -0.19) Finished aligning to item in 1 rounds Trimming A by 23 105 90 20, reference by 23 100 90 20 -24.1 -4.4 unbinned pixels; 15.2 93.4 nm along two shift axes; 22.3 -92.9 nm on specimen axes Total_Shift = 49.027702 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.52 microns changed by -2.12 to target drift = 0.20 nm/sec Measured defocus = -1.67 microns changed by 0.07 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0428.tif 27 frames are being saved to U:\krios\20190717\PilA_0429.tif 27 frames are being saved to U:\krios\20190717\PilA_0430.tif 27 frames are being saved to U:\krios\20190717\PilA_0431.tif 27 frames are being saved to U:\krios\20190717\PilA_0432.tif 27 frames are being saved to U:\krios\20190717\PilA_0433.tif Running script 17 at item 122 (284.08, -256.42) Remaining time to fill dewars is 6900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 40 20 20, reference by 20 160 150 20 Disparity in stage position after aligning to target position: 0.16 -0.56 (2nd move error 0.01 -0.25) Finished aligning to item in 1 rounds Trimming A by 20 40 20 20, reference by 20 40 20 20 -30.8 1.0 unbinned pixels; 43.8 109.3 nm along two shift axes; 2.7 -119.9 nm on specimen axes Total_Shift = 61.585203 Trimming A by 20 45 30 20, reference by 20 40 20 20 -5.8 -0.5 unbinned pixels; 5.8 21.4 nm along two shift axes; 3.1 -22.3 nm on specimen axes Total_Shift = 11.55613 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.32 microns changed by -2.02 to target drift = 0.11 nm/sec Measured defocus = -1.71 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0434.tif 27 frames are being saved to U:\krios\20190717\PilA_0435.tif 27 frames are being saved to U:\krios\20190717\PilA_0436.tif 27 frames are being saved to U:\krios\20190717\PilA_0437.tif 27 frames are being saved to U:\krios\20190717\PilA_0438.tif 27 frames are being saved to U:\krios\20190717\PilA_0439.tif Running script 17 at item 123 (281.62, -255.27) Remaining time to fill dewars is 6720 Dewars are NOT busy filling Trimming A by 20 165 20 185, reference by 230 21 20 210 Trimming A by 20 165 20 185, reference by 229 20 185 20 Trimming A by 20 165 20 185, reference by 21 165 55 170 Trimming A by 20 165 20 185, reference by 20 160 150 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.29 Trimming A by 20 20 20 20, reference by 21 165 55 170 Disparity in stage position after aligning to target position: 0.10 -0.06 (2nd move error 0.02 0.21) Finished aligning to item in 2 rounds -4.5 0.7 unbinned pixels; 8.4 15.0 nm along two shift axes; -1.8 -17.5 nm on specimen axes Total_Shift = 9.017946 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.13 microns changed by -1.93 to target drift = 0.11 nm/sec Measured defocus = -1.77 microns changed by -0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0440.tif 27 frames are being saved to U:\krios\20190717\PilA_0441.tif 27 frames are being saved to U:\krios\20190717\PilA_0442.tif 27 frames are being saved to U:\krios\20190717\PilA_0443.tif 27 frames are being saved to U:\krios\20190717\PilA_0444.tif 27 frames are being saved to U:\krios\20190717\PilA_0445.tif Running script 17 at item 124 (279.14, -253.63) Remaining time to fill dewars is 6600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 46 21 20 95, reference by 21 165 55 170 Disparity in stage position after aligning to target position: 0.09 -0.22 (2nd move error 0.01 0.05) Finished aligning to item in 1 rounds Trimming A by 46 21 20 95, reference by 46 21 20 95 -1.5 -1.2 unbinned pixels; 2.6 7.1 nm along two shift axes; 5.1 -5.4 nm on specimen axes Total_Shift = 3.810078 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.26 microns changed by -2.16 to target drift = 0.72 nm/sec Measured defocus = -2.23 microns changed by 0.33 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0446.tif 27 frames are being saved to U:\krios\20190717\PilA_0447.tif 27 frames are being saved to U:\krios\20190717\PilA_0448.tif 27 frames are being saved to U:\krios\20190717\PilA_0449.tif 27 frames are being saved to U:\krios\20190717\PilA_0450.tif 27 frames are being saved to U:\krios\20190717\PilA_0451.tif Running script 17 at item 125 (276.31, -252.00) Remaining time to fill dewars is 6420 Dewars are NOT busy filling Trimming A by 245 22 20 225, reference by 230 21 20 210 Trimming A by 245 22 20 225, reference by 229 20 185 20 Trimming A by 245 22 20 225, reference by 21 165 55 170 Trimming A by 245 22 20 225, reference by 20 160 150 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.28 Trimming A by 96 22 20 185, reference by 230 21 20 210 Disparity in stage position after aligning to target position: 0.39 -0.20 (2nd move error 0.21 0.05) Finished aligning to item in 2 rounds Trimming A by 96 22 20 180, reference by 96 22 20 185 -1.7 -7.0 unbinned pixels; 23.5 16.8 nm along two shift axes; 27.6 -5.3 nm on specimen axes Total_Shift = 14.415271 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = -0.30 microns changed by -1.70 to target drift = 0.16 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0452.tif 27 frames are being saved to U:\krios\20190717\PilA_0453.tif 27 frames are being saved to U:\krios\20190717\PilA_0454.tif 27 frames are being saved to U:\krios\20190717\PilA_0455.tif 27 frames are being saved to U:\krios\20190717\PilA_0456.tif 27 frames are being saved to U:\krios\20190717\PilA_0457.tif Running script 17 at item 126 (277.49, -256.18) Remaining time to fill dewars is 6180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 65 20 110, reference by 21 165 55 170 Disparity in stage position after aligning to target position: 0.17 -0.28 (2nd move error 0.09 -0.02) Finished aligning to item in 1 rounds Trimming A by 30 65 20 110, reference by 35 65 20 110 -2.7 -1.7 unbinned pixels; 2.8 12.3 nm along two shift axes; 7.3 -10.1 nm on specimen axes Total_Shift = 6.374711 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = -0.34 microns changed by -1.76 to target drift = 0.12 nm/sec Measured defocus = -2.09 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0458.tif 27 frames are being saved to U:\krios\20190717\PilA_0459.tif 27 frames are being saved to U:\krios\20190717\PilA_0460.tif 27 frames are being saved to U:\krios\20190717\PilA_0461.tif 27 frames are being saved to U:\krios\20190717\PilA_0462.tif 27 frames are being saved to U:\krios\20190717\PilA_0463.tif Running script 17 at item 127 (280.02, -257.94) Remaining time to fill dewars is 6000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 85 20 95, reference by 21 165 55 170 Disparity in stage position after aligning to target position: 0.21 -0.13 (2nd move error 0.12 0.13) Finished aligning to item in 1 rounds Trimming A by 21 85 40 85, reference by 20 85 20 95 0.1 -3.0 unbinned pixels; 11.0 4.2 nm along two shift axes; 11.5 1.0 nm on specimen axes Total_Shift = 5.930848 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.40 microns changed by -1.80 to target drift = 0.16 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0464.tif 27 frames are being saved to U:\krios\20190717\PilA_0465.tif 27 frames are being saved to U:\krios\20190717\PilA_0466.tif 27 frames are being saved to U:\krios\20190717\PilA_0467.tif 27 frames are being saved to U:\krios\20190717\PilA_0468.tif 27 frames are being saved to U:\krios\20190717\PilA_0469.tif Running script 17 at item 128 (282.46, -259.59) Remaining time to fill dewars is 5820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 125 20 110, reference by 21 165 55 170 Disparity in stage position after aligning to target position: 0.21 -0.01 (2nd move error 0.12 0.26) Finished aligning to item in 1 rounds Trimming A by 20 120 70 75, reference by 24 125 20 110 -2.9 4.6 unbinned pixels; 20.7 3.7 nm along two shift axes; -17.2 -12.4 nm on specimen axes Total_Shift = 10.880811 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.41 microns changed by -1.89 to target drift = 0.11 nm/sec Measured defocus = -2.32 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0470.tif 27 frames are being saved to U:\krios\20190717\PilA_0471.tif 27 frames are being saved to U:\krios\20190717\PilA_0472.tif 27 frames are being saved to U:\krios\20190717\PilA_0473.tif 27 frames are being saved to U:\krios\20190717\PilA_0474.tif 27 frames are being saved to U:\krios\20190717\PilA_0475.tif Running script 17 at item 129 (284.87, -260.76) Remaining time to fill dewars is 5640 Dewars are NOT busy filling Trimming A by 22 155 155 20, reference by 230 21 20 210 Trimming A by 22 155 155 20, reference by 229 20 185 20 Trimming A by 22 155 155 20, reference by 21 165 55 170 Trimming A by 22 155 155 20, reference by 20 160 150 20 Disparity in stage position after aligning to center of map frame: 0.16 -0.31 Trimming A by 20 130 65 80, reference by 20 160 150 20 Disparity in stage position after aligning to target position: 0.25 -0.53 (2nd move error 0.09 -0.24) Finished aligning to item in 2 rounds Trimming A by 20 130 70 80, reference by 20 130 65 80 -29.2 -2.8 unbinned pixels; 27.8 109.2 nm along two shift axes; 17.0 -112.9 nm on specimen axes Total_Shift = 58.599166 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.38 microns changed by -2.01 to target drift = 0.20 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0476.tif 27 frames are being saved to U:\krios\20190717\PilA_0477.tif 27 frames are being saved to U:\krios\20190717\PilA_0478.tif 27 frames are being saved to U:\krios\20190717\PilA_0479.tif 27 frames are being saved to U:\krios\20190717\PilA_0480.tif 27 frames are being saved to U:\krios\20190717\PilA_0481.tif Running script 17 at item 130 (260.52, -307.05) Remaining time to fill dewars is 5520 Dewars are NOT busy filling Trimming A by 230 24 175 20, reference by 185 22 20 200 Trimming A by 230 24 175 20, reference by 215 24 165 20 Trimming A by 230 24 175 20, reference by 23 135 20 150 Trimming A by 230 24 175 20, reference by 24 130 145 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.37 Trimming A by 111 21 175 20, reference by 215 24 165 20 Disparity in stage position after aligning to target position: 0.14 -0.17 (2nd move error 0.09 0.17) Finished aligning to item in 2 rounds Trimming A by 130 20 165 20, reference by 111 21 175 20 -4.2 -0.9 unbinned pixels; 2.3 16.3 nm along two shift axes; 4.2 -16.0 nm on specimen axes Total_Shift = 8.50532 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.73 microns changed by -1.77 to target drift = 0.14 nm/sec Measured defocus = -2.44 microns changed by -0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0482.tif 27 frames are being saved to U:\krios\20190717\PilA_0483.tif 27 frames are being saved to U:\krios\20190717\PilA_0484.tif 27 frames are being saved to U:\krios\20190717\PilA_0485.tif 27 frames are being saved to U:\krios\20190717\PilA_0486.tif 27 frames are being saved to U:\krios\20190717\PilA_0487.tif Running script 17 at item 131 (258.02, -305.39) Remaining time to fill dewars is 5340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 20 135 20, reference by 215 24 165 20 Disparity in stage position after aligning to target position: 0.13 -0.40 (2nd move error 0.08 -0.06) Finished aligning to item in 1 rounds Trimming A by 140 20 135 20, reference by 140 20 135 20 -6.5 -5.3 unbinned pixels; 11.0 31.5 nm along two shift axes; 22.1 -24.3 nm on specimen axes Total_Shift = 16.853237 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.90 microns changed by -1.70 to target drift = 0.18 nm/sec Measured defocus = -2.58 microns changed by -0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0488.tif 27 frames are being saved to U:\krios\20190717\PilA_0489.tif 27 frames are being saved to U:\krios\20190717\PilA_0490.tif 27 frames are being saved to U:\krios\20190717\PilA_0491.tif 27 frames are being saved to U:\krios\20190717\PilA_0492.tif 27 frames are being saved to U:\krios\20190717\PilA_0493.tif Running script 17 at item 132 (255.54, -303.68) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 155 21 115 20, reference by 215 24 165 20 Disparity in stage position after aligning to target position: 0.11 -0.47 (2nd move error 0.06 -0.13) Finished aligning to item in 1 rounds Trimming A by 150 21 120 20, reference by 155 21 115 20 -17.5 -3.7 unbinned pixels; 9.2 68.5 nm along two shift axes; 18.1 -67.2 nm on specimen axes Total_Shift = 35.711204 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.44 microns changed by -1.74 to target drift = 0.17 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0494.tif 27 frames are being saved to U:\krios\20190717\PilA_0495.tif 27 frames are being saved to U:\krios\20190717\PilA_0496.tif 27 frames are being saved to U:\krios\20190717\PilA_0497.tif 27 frames are being saved to U:\krios\20190717\PilA_0498.tif 27 frames are being saved to U:\krios\20190717\PilA_0499.tif Running script 17 at item 133 (253.00, -301.92) Remaining time to fill dewars is 4920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 175 22 100 20, reference by 215 24 165 20 Disparity in stage position after aligning to target position: 0.06 -0.59 (2nd move error 0.01 -0.25) Finished aligning to item in 1 rounds Trimming A by 170 22 105 20, reference by 175 22 100 20 -31.6 1.2 unbinned pixels; 45.7 112.0 nm along two shift axes; 1.9 -123.2 nm on specimen axes Total_Shift = 63.276822 Trimming A by 165 24 105 20, reference by 175 22 100 20 -4.4 -0.1 unbinned pixels; 5.4 15.9 nm along two shift axes; 1.3 -17.0 nm on specimen axes Total_Shift = 8.729876 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.29 microns changed by -1.69 to target drift = 0.11 nm/sec Measured defocus = -1.39 microns changed by -0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0500.tif 27 frames are being saved to U:\krios\20190717\PilA_0501.tif 27 frames are being saved to U:\krios\20190717\PilA_0502.tif 27 frames are being saved to U:\krios\20190717\PilA_0503.tif 27 frames are being saved to U:\krios\20190717\PilA_0504.tif 27 frames are being saved to U:\krios\20190717\PilA_0505.tif Running script 17 at item 134 (248.91, -303.39) Remaining time to fill dewars is 4860 Dewars are NOT busy filling Trimming A by 215 20 20 200, reference by 185 22 20 200 Trimming A by 215 20 20 200, reference by 215 24 165 20 Trimming A by 215 20 20 200, reference by 23 135 20 150 Trimming A by 215 20 20 200, reference by 24 130 145 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.26 Trimming A by 155 20 20 120, reference by 185 22 20 200 Disparity in stage position after aligning to target position: 0.20 -0.09 (2nd move error 0.16 0.15) Finished aligning to item in 2 rounds Trimming A by 155 20 20 120, reference by 155 20 20 120 1.2 -1.9 unbinned pixels; 8.5 1.6 nm along two shift axes; 7.0 5.2 nm on specimen axes Total_Shift = 4.465325 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.00 microns changed by -1.50 to target drift = 0.16 nm/sec Measured defocus = -1.48 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0506.tif 27 frames are being saved to U:\krios\20190717\PilA_0507.tif 27 frames are being saved to U:\krios\20190717\PilA_0508.tif 27 frames are being saved to U:\krios\20190717\PilA_0509.tif 27 frames are being saved to U:\krios\20190717\PilA_0510.tif 27 frames are being saved to U:\krios\20190717\PilA_0511.tif Running script 17 at item 135 (251.62, -304.61) Remaining time to fill dewars is 4620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 20 55 55, reference by 185 22 20 200 Disparity in stage position after aligning to target position: 0.22 -0.04 (2nd move error 0.18 0.19) Finished aligning to item in 1 rounds Trimming A by 100 20 55 45, reference by 90 20 55 55 1.0 -2.7 unbinned pixels; 11.4 0.6 nm along two shift axes; 10.4 4.4 nm on specimen axes Total_Shift = 5.817856 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.03 microns changed by -1.63 to target drift = 0.14 nm/sec Measured defocus = -1.65 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0512.tif 27 frames are being saved to U:\krios\20190717\PilA_0513.tif 27 frames are being saved to U:\krios\20190717\PilA_0514.tif 27 frames are being saved to U:\krios\20190717\PilA_0515.tif 27 frames are being saved to U:\krios\20190717\PilA_0516.tif 27 frames are being saved to U:\krios\20190717\PilA_0517.tif Running script 17 at item 136 (256.38, -307.94) Remaining time to fill dewars is 4440 Dewars are NOT busy filling Trimming A by 230 24 175 20, reference by 185 22 20 200 Trimming A by 230 24 175 20, reference by 215 24 165 20 Trimming A by 230 24 175 20, reference by 23 135 20 150 Trimming A by 230 24 175 20, reference by 24 130 145 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.28 Trimming A by 70 20 65 20, reference by 215 24 165 20 Disparity in stage position after aligning to target position: 0.12 -0.43 (2nd move error 0.07 -0.17) Finished aligning to item in 2 rounds Trimming A by 65 20 70 20, reference by 70 20 65 20 -19.6 -4.8 unbinned pixels; 7.7 77.7 nm along two shift axes; 23.0 -75.1 nm on specimen axes Total_Shift = 40.297269 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = -0.06 microns changed by -1.64 to target drift = 0.21 nm/sec Measured defocus = -1.66 microns changed by -0.04 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0518.tif 27 frames are being saved to U:\krios\20190717\PilA_0519.tif 27 frames are being saved to U:\krios\20190717\PilA_0520.tif 27 frames are being saved to U:\krios\20190717\PilA_0521.tif 27 frames are being saved to U:\krios\20190717\PilA_0522.tif 27 frames are being saved to U:\krios\20190717\PilA_0523.tif Running script 17 at item 137 (259.14, -309.54) Remaining time to fill dewars is 4200 Dewars are NOT busy filling Trimming A by 20 140 140 20, reference by 185 22 20 200 Trimming A by 20 140 140 20, reference by 215 24 165 20 Trimming A by 20 140 140 20, reference by 23 135 20 150 Trimming A by 20 140 140 20, reference by 24 130 145 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.41 Trimming A by 42 42 115 20, reference by 24 130 145 20 Disparity in stage position after aligning to target position: -0.02 -0.31 (2nd move error -0.10 0.07) Finished aligning to item in 2 rounds Trimming A by 55 55 105 20, reference by 42 42 115 20 -2.4 18.5 unbinned pixels; 71.0 19.3 nm along two shift axes; -71.4 -13.1 nm on specimen axes Total_Shift = 37.246426 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = -0.08 microns changed by -1.72 to target drift = 0.13 nm/sec Measured defocus = -1.77 microns changed by -0.03 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0524.tif 27 frames are being saved to U:\krios\20190717\PilA_0525.tif 27 frames are being saved to U:\krios\20190717\PilA_0526.tif 27 frames are being saved to U:\krios\20190717\PilA_0527.tif 27 frames are being saved to U:\krios\20190717\PilA_0528.tif 27 frames are being saved to U:\krios\20190717\PilA_0529.tif Running script 17 at item 138 (257.49, -312.11) Remaining time to fill dewars is 4080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 52 100 20, reference by 24 130 145 20 Disparity in stage position after aligning to target position: -0.01 -0.46 (2nd move error -0.09 -0.08) Finished aligning to item in 1 rounds Trimming A by 20 52 100 20, reference by 20 52 100 20 -7.6 16.2 unbinned pixels; 69.4 3.1 nm along two shift axes; -61.3 -33.0 nm on specimen axes Total_Shift = 35.734995 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = -0.28 microns changed by -1.62 to target drift = 0.04 nm/sec Measured defocus = -1.81 microns changed by -0.09 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0530.tif 27 frames are being saved to U:\krios\20190717\PilA_0531.tif 27 frames are being saved to U:\krios\20190717\PilA_0532.tif 27 frames are being saved to U:\krios\20190717\PilA_0533.tif 27 frames are being saved to U:\krios\20190717\PilA_0534.tif 27 frames are being saved to U:\krios\20190717\PilA_0535.tif Running script 17 at item 139 (254.93, -310.33) Remaining time to fill dewars is 3900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 130 145 20 Disparity in stage position after aligning to target position: -0.06 -0.58 (2nd move error -0.12 -0.19) Finished aligning to item in 1 rounds -21.5 23.3 unbinned pixels; 114.0 42.4 nm along two shift axes; -86.3 -88.7 nm on specimen axes Total_Shift = 63.53359 -3.3 14.1 unbinned pixels; 56.1 9.2 nm along two shift axes; -54.1 -15.9 nm on specimen axes Total_Shift = 28.947835 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = -0.33 microns changed by -1.67 to target drift = 0.04 nm/sec Measured defocus = -2.03 microns changed by 0.03 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0536.tif 27 frames are being saved to U:\krios\20190717\PilA_0537.tif 27 frames are being saved to U:\krios\20190717\PilA_0538.tif 27 frames are being saved to U:\krios\20190717\PilA_0539.tif 27 frames are being saved to U:\krios\20190717\PilA_0540.tif 27 frames are being saved to U:\krios\20190717\PilA_0541.tif Running script 17 at item 140 (245.68, -308.43) Remaining time to fill dewars is 3720 Dewars are NOT busy filling Trimming A by 205 24 20 210, reference by 185 22 20 200 Trimming A by 205 24 20 210, reference by 215 24 165 20 Trimming A by 205 24 20 210, reference by 23 135 20 150 Trimming A by 205 24 20 210, reference by 24 130 145 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.28 Trimming A by 81 24 20 170, reference by 185 22 20 200 Disparity in stage position after aligning to target position: 0.24 -0.29 (2nd move error 0.20 -0.04) Finished aligning to item in 2 rounds Trimming A by 101 21 20 150, reference by 81 24 20 170 -4.1 -7.3 unbinned pixels; 21.6 25.9 nm along two shift axes; 29.5 -14.5 nm on specimen axes Total_Shift = 16.850062 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = -0.75 microns changed by -1.35 to target drift = 0.18 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0542.tif 27 frames are being saved to U:\krios\20190717\PilA_0543.tif 27 frames are being saved to U:\krios\20190717\PilA_0544.tif 27 frames are being saved to U:\krios\20190717\PilA_0545.tif 27 frames are being saved to U:\krios\20190717\PilA_0546.tif 27 frames are being saved to U:\krios\20190717\PilA_0547.tif Running script 17 at item 141 (248.41, -310.15) Remaining time to fill dewars is 3480 Dewars are NOT busy filling Trimming A by 21 140 55 150, reference by 185 22 20 200 Trimming A by 21 140 55 150, reference by 215 24 165 20 Trimming A by 21 140 55 150, reference by 23 135 20 150 Trimming A by 21 140 55 150, reference by 24 130 145 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.33 Trimming A by 40 20 20 70, reference by 23 135 20 150 Disparity in stage position after aligning to target position: 0.01 -0.20 (2nd move error -0.08 0.11) Finished aligning to item in 2 rounds Trimming A by 40 20 20 70, reference by 40 20 20 70 -2.6 28.1 unbinned pixels; 106.7 33.0 nm along two shift axes; -108.8 -15.9 nm on specimen axes Total_Shift = 56.447761 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.76 microns changed by -1.44 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0548.tif 27 frames are being saved to U:\krios\20190717\PilA_0549.tif 27 frames are being saved to U:\krios\20190717\PilA_0550.tif 27 frames are being saved to U:\krios\20190717\PilA_0551.tif 27 frames are being saved to U:\krios\20190717\PilA_0552.tif 27 frames are being saved to U:\krios\20190717\PilA_0553.tif Running script 17 at item 142 (250.93, -311.87) Remaining time to fill dewars is 3360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 135 20 150 Disparity in stage position after aligning to target position: 0.00 -0.08 (2nd move error -0.09 0.22) Finished aligning to item in 1 rounds 3.6 17.9 unbinned pixels; 61.2 39.9 nm along two shift axes; -70.5 10.3 nm on specimen axes Total_Shift = 36.548591 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.85 microns changed by -1.45 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0554.tif 27 frames are being saved to U:\krios\20190717\PilA_0555.tif 27 frames are being saved to U:\krios\20190717\PilA_0556.tif 27 frames are being saved to U:\krios\20190717\PilA_0557.tif 27 frames are being saved to U:\krios\20190717\PilA_0558.tif 27 frames are being saved to U:\krios\20190717\PilA_0559.tif Running script 17 at item 143 (253.34, -312.98) Remaining time to fill dewars is 3240 Dewars are NOT busy filling Trimming A by 23 126 155 20, reference by 185 22 20 200 Trimming A by 23 126 155 20, reference by 215 24 165 20 Trimming A by 23 126 155 20, reference by 23 135 20 150 Trimming A by 23 126 155 20, reference by 24 130 145 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.32 Trimming A by 20 20 20 20, reference by 24 130 145 20 Disparity in stage position after aligning to target position: -0.04 -0.64 (2nd move error -0.09 -0.34) Finished aligning to item in 2 rounds -27.0 16.2 unbinned pixels; 95.0 72.8 nm along two shift axes; -57.5 -108.5 nm on specimen axes Total_Shift = 63.051793 1.0 9.4 unbinned pixels; 33.2 17.6 nm along two shift axes; -36.6 1.8 nm on specimen axes Total_Shift = 18.832627 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.83 microns changed by -1.57 to target drift = 0.06 nm/sec Measured defocus = -2.36 microns changed by -0.04 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0560.tif 27 frames are being saved to U:\krios\20190717\PilA_0561.tif 27 frames are being saved to U:\krios\20190717\PilA_0562.tif 27 frames are being saved to U:\krios\20190717\PilA_0563.tif 27 frames are being saved to U:\krios\20190717\PilA_0564.tif 27 frames are being saved to U:\krios\20190717\PilA_0565.tif Running script 17 at item 144 (254.19, -317.29) Remaining time to fill dewars is 3060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 100 60 55, reference by 24 130 145 20 Disparity in stage position after aligning to target position: 0.09 -0.61 (2nd move error 0.04 -0.31) Finished aligning to item in 1 rounds Trimming A by 20 110 60 45, reference by 20 100 60 55 -26.0 -4.0 unbinned pixels; 19.2 99.4 nm along two shift axes; 21.0 -100.1 nm on specimen axes Total_Shift = 52.522039 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.99 microns changed by -1.51 to target drift = 0.25 nm/sec Measured defocus = -2.42 microns changed by -0.08 to target drift = 0.16 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0566.tif 27 frames are being saved to U:\krios\20190717\PilA_0567.tif 27 frames are being saved to U:\krios\20190717\PilA_0568.tif 27 frames are being saved to U:\krios\20190717\PilA_0569.tif 27 frames are being saved to U:\krios\20190717\PilA_0570.tif 27 frames are being saved to U:\krios\20190717\PilA_0571.tif Running script 17 at item 145 (256.73, -318.98) Remaining time to fill dewars is 2940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 175 125 20, reference by 24 130 145 20 Disparity in stage position after aligning to target position: 0.12 -0.45 (2nd move error 0.08 -0.16) Finished aligning to item in 1 rounds Trimming A by 20 180 125 20, reference by 20 175 125 20 -17.6 -4.3 unbinned pixels; 7.3 69.9 nm along two shift axes; 20.4 -67.6 nm on specimen axes Total_Shift = 36.253789 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.98 microns changed by -1.62 to target drift = 0.21 nm/sec Measured defocus = -2.60 microns changed by -0.00 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0572.tif 27 frames are being saved to U:\krios\20190717\PilA_0573.tif 27 frames are being saved to U:\krios\20190717\PilA_0574.tif 27 frames are being saved to U:\krios\20190717\PilA_0575.tif 27 frames are being saved to U:\krios\20190717\PilA_0576.tif 27 frames are being saved to U:\krios\20190717\PilA_0577.tif Running script 17 at item 146 (251.73, -316.14) Remaining time to fill dewars is 2700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 90 20 80, reference by 23 135 20 150 Disparity in stage position after aligning to target position: 0.08 -0.09 (2nd move error -0.02 0.21) Finished aligning to item in 1 rounds Trimming A by 24 85 20 75, reference by 24 90 20 80 0.8 5.2 unbinned pixels; 18.3 10.7 nm along two shift axes; -20.6 2.0 nm on specimen axes Total_Shift = 10.615542 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.25 microns changed by -1.55 to target drift = 0.13 nm/sec Measured defocus = -1.21 microns changed by -0.09 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0578.tif 27 frames are being saved to U:\krios\20190717\PilA_0579.tif 27 frames are being saved to U:\krios\20190717\PilA_0580.tif 27 frames are being saved to U:\krios\20190717\PilA_0581.tif 27 frames are being saved to U:\krios\20190717\PilA_0582.tif 27 frames are being saved to U:\krios\20190717\PilA_0583.tif Running script 17 at item 147 (249.19, -314.44) Remaining time to fill dewars is 2460 Dewars are NOT busy filling Trimming A by 24 135 20 155, reference by 185 22 20 200 Trimming A by 24 135 20 155, reference by 215 24 165 20 Trimming A by 24 135 20 155, reference by 23 135 20 150 Trimming A by 24 135 20 155, reference by 24 130 145 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.32 Trimming A by 20 80 20 85, reference by 23 135 20 150 Disparity in stage position after aligning to target position: 0.09 -0.15 (2nd move error 0.03 0.14) Finished aligning to item in 2 rounds Trimming A by 20 75 20 85, reference by 20 80 20 85 -3.6 -0.4 unbinned pixels; 3.3 13.4 nm along two shift axes; 2.2 -13.8 nm on specimen axes Total_Shift = 7.192865 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.12 microns changed by -1.52 to target drift = 0.13 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0584.tif 27 frames are being saved to U:\krios\20190717\PilA_0585.tif 27 frames are being saved to U:\krios\20190717\PilA_0586.tif 27 frames are being saved to U:\krios\20190717\PilA_0587.tif 27 frames are being saved to U:\krios\20190717\PilA_0588.tif 27 frames are being saved to U:\krios\20190717\PilA_0589.tif Running script 17 at item 148 (246.75, -312.73) Remaining time to fill dewars is 2400 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 21 65 20 100, reference by 23 135 20 150 Disparity in stage position after aligning to target position: -0.00 -0.28 (2nd move error -0.06 0.01) Finished aligning to item in 1 rounds Trimming A by 21 65 20 100, reference by 21 65 20 100 2.4 13.4 unbinned pixels; 46.1 28.6 nm along two shift axes; -52.6 6.3 nm on specimen axes Total_Shift = 27.174445 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.12 microns changed by -1.62 to target drift = 2.58 nm/sec Measured defocus = -1.64 microns changed by 0.14 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0590.tif 27 frames are being saved to U:\krios\20190717\PilA_0591.tif 27 frames are being saved to U:\krios\20190717\PilA_0592.tif 27 frames are being saved to U:\krios\20190717\PilA_0593.tif 27 frames are being saved to U:\krios\20190717\PilA_0594.tif 27 frames are being saved to U:\krios\20190717\PilA_0595.tif Running script 17 at item 149 (244.25, -310.95) Remaining time to fill dewars is 2220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 68 22 20 175, reference by 23 135 20 150 Disparity in stage position after aligning to target position: -0.12 -0.40 (2nd move error -0.19 -0.10) Finished aligning to item in 1 rounds Trimming A by 76 22 20 170, reference by 68 22 20 175 -8.1 35.1 unbinned pixels; 139.6 23.8 nm along two shift axes; -134.9 -38.8 nm on specimen axes Total_Shift = 72.050006 Trimming A by 64 22 20 180, reference by 68 22 20 175 -0.8 23.0 unbinned pixels; 85.4 31.8 nm along two shift axes; -89.2 -7.8 nm on specimen axes Total_Shift = 45.955737 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = -0.29 microns changed by -1.31 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0596.tif 27 frames are being saved to U:\krios\20190717\PilA_0597.tif 27 frames are being saved to U:\krios\20190717\PilA_0598.tif 27 frames are being saved to U:\krios\20190717\PilA_0599.tif 27 frames are being saved to U:\krios\20190717\PilA_0600.tif 27 frames are being saved to U:\krios\20190717\PilA_0601.tif Running script 17 at item 150 (245.11, -315.31) Remaining time to fill dewars is 2040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 130 20 170, reference by 23 135 20 150 Disparity in stage position after aligning to target position: 0.05 -0.37 (2nd move error -0.02 -0.07) Finished aligning to item in 1 rounds Trimming A by 21 130 20 170, reference by 21 130 20 170 -7.8 3.7 unbinned pixels; 23.8 22.5 nm along two shift axes; -12.8 -31.1 nm on specimen axes Total_Shift = 17.254656 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = -0.34 microns changed by -1.36 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0602.tif 27 frames are being saved to U:\krios\20190717\PilA_0603.tif 27 frames are being saved to U:\krios\20190717\PilA_0604.tif 27 frames are being saved to U:\krios\20190717\PilA_0605.tif 27 frames are being saved to U:\krios\20190717\PilA_0606.tif 27 frames are being saved to U:\krios\20190717\PilA_0607.tif Running script 17 at item 151 (247.58, -317.02) Remaining time to fill dewars is 1920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 150 20 150, reference by 23 135 20 150 Disparity in stage position after aligning to target position: 0.06 -0.23 (2nd move error 0.01 0.06) Finished aligning to item in 1 rounds Trimming A by 21 150 20 145, reference by 21 150 20 150 0.0 0.1 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.3 0.1 nm on specimen axes Total_Shift = 0.134462 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = -0.41 microns changed by -1.39 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0608.tif 27 frames are being saved to U:\krios\20190717\PilA_0609.tif 27 frames are being saved to U:\krios\20190717\PilA_0610.tif 27 frames are being saved to U:\krios\20190717\PilA_0611.tif 27 frames are being saved to U:\krios\20190717\PilA_0612.tif 27 frames are being saved to U:\krios\20190717\PilA_0613.tif Running script 17 at item 152 (250.12, -318.65) Remaining time to fill dewars is 1680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 165 20 135, reference by 23 135 20 150 Disparity in stage position after aligning to target position: 0.10 -0.11 (2nd move error 0.04 0.18) Finished aligning to item in 1 rounds Trimming A by 21 165 80 125, reference by 22 165 20 135 -2.1 2.2 unbinned pixels; 11.0 4.3 nm along two shift axes; -8.3 -8.8 nm on specimen axes Total_Shift = 6.196715 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = -0.50 microns changed by -1.40 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0614.tif 27 frames are being saved to U:\krios\20190717\PilA_0615.tif 27 frames are being saved to U:\krios\20190717\PilA_0616.tif 27 frames are being saved to U:\krios\20190717\PilA_0617.tif 27 frames are being saved to U:\krios\20190717\PilA_0618.tif 27 frames are being saved to U:\krios\20190717\PilA_0619.tif Running script 17 at item 153 (252.65, -320.04) Remaining time to fill dewars is 1560 Dewars are NOT busy filling Trimming A by 23 140 20 155, reference by 185 22 20 200 Trimming A by 23 140 20 155, reference by 215 24 165 20 Trimming A by 23 140 20 155, reference by 23 135 20 150 Trimming A by 23 140 20 155, reference by 24 130 145 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.31 Trimming A by 24 185 20 110, reference by 23 135 20 150 Disparity in stage position after aligning to target position: 0.19 0.07 (2nd move error 0.09 0.36) Finished aligning to item in 2 rounds Trimming A by 20 170 20 110, reference by 24 185 20 110 -6.7 1.4 unbinned pixels; 13.8 22.2 nm along two shift axes; -3.9 -26.5 nm on specimen axes Total_Shift = 13.751411 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = -0.50 microns changed by -1.50 to target drift = 0.14 nm/sec Measured defocus = -1.94 microns changed by -0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0620.tif 27 frames are being saved to U:\krios\20190717\PilA_0621.tif 27 frames are being saved to U:\krios\20190717\PilA_0622.tif 27 frames are being saved to U:\krios\20190717\PilA_0623.tif 27 frames are being saved to U:\krios\20190717\PilA_0624.tif 27 frames are being saved to U:\krios\20190717\PilA_0625.tif Running script 17 at item 154 (206.18, -275.81) Remaining time to fill dewars is 1260 Dewars are NOT busy filling Trimming A by 266 23 170 20, reference by 219 20 20 240 Trimming A by 266 23 170 20, reference by 245 22 160 20 Trimming A by 266 23 170 20, reference by 23 90 20 135 Trimming A by 266 23 170 20, reference by 23 95 90 20 Disparity in stage position after aligning to center of map frame: -0.00 -0.35 Trimming A by 125 24 145 20, reference by 245 22 160 20 Disparity in stage position after aligning to target position: 0.08 -0.21 (2nd move error 0.07 0.12) Finished aligning to item in 2 rounds Trimming A by 130 23 145 20, reference by 125 24 145 20 -1.7 -3.7 unbinned pixels; 11.3 11.8 nm along two shift axes; 14.7 -6.0 nm on specimen axes Total_Shift = 8.146259 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.21 microns changed by -2.31 to target drift = 0.20 nm/sec Measured defocus = -2.11 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0626.tif 27 frames are being saved to U:\krios\20190717\PilA_0627.tif 27 frames are being saved to U:\krios\20190717\PilA_0628.tif 27 frames are being saved to U:\krios\20190717\PilA_0629.tif 27 frames are being saved to U:\krios\20190717\PilA_0630.tif 27 frames are being saved to U:\krios\20190717\PilA_0631.tif Running script 17 at item 155 (203.65, -274.10) Remaining time to fill dewars is 1140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 20 125 20, reference by 245 22 160 20 Disparity in stage position after aligning to target position: 0.10 -0.41 (2nd move error 0.09 -0.08) Finished aligning to item in 1 rounds Trimming A by 140 20 125 20, reference by 140 20 125 20 -8.8 -2.3 unbinned pixels; 3.0 35.1 nm along two shift axes; 10.8 -33.7 nm on specimen axes Total_Shift = 18.154546 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.01 microns changed by -2.19 to target drift = 0.11 nm/sec Measured defocus = -2.17 microns changed by -0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0632.tif 27 frames are being saved to U:\krios\20190717\PilA_0633.tif 27 frames are being saved to U:\krios\20190717\PilA_0634.tif 27 frames are being saved to U:\krios\20190717\PilA_0635.tif 27 frames are being saved to U:\krios\20190717\PilA_0636.tif 27 frames are being saved to U:\krios\20190717\PilA_0637.tif Running script 17 at item 156 (201.20, -272.41) Remaining time to fill dewars is 1020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 155 22 110 20, reference by 245 22 160 20 Disparity in stage position after aligning to target position: 0.11 -0.49 (2nd move error 0.11 -0.17) Finished aligning to item in 1 rounds Trimming A by 150 23 115 20, reference by 155 22 110 20 -16.1 -1.2 unbinned pixels; 16.5 59.7 nm along two shift axes; 8.1 -62.2 nm on specimen axes Total_Shift = 32.217515 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.11 microns changed by -2.19 to target drift = 0.15 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0638.tif 27 frames are being saved to U:\krios\20190717\PilA_0639.tif 27 frames are being saved to U:\krios\20190717\PilA_0640.tif 27 frames are being saved to U:\krios\20190717\PilA_0641.tif 27 frames are being saved to U:\krios\20190717\PilA_0642.tif 27 frames are being saved to U:\krios\20190717\PilA_0643.tif Running script 17 at item 157 (194.51, -272.13) Remaining time to fill dewars is 900 Dewars are NOT busy filling Trimming A by 241 21 20 245, reference by 219 20 20 240 Trimming A by 241 21 20 245, reference by 245 22 160 20 Trimming A by 241 21 20 245, reference by 23 90 20 135 Trimming A by 241 21 20 245, reference by 23 95 90 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.22 Trimming A by 170 24 20 140, reference by 219 20 20 240 Disparity in stage position after aligning to target position: 0.21 -0.12 (2nd move error 0.17 0.08) Finished aligning to item in 2 rounds Trimming A by 165 23 20 140, reference by 170 24 20 140 0.4 1.2 unbinned pixels; 3.7 3.3 nm along two shift axes; -4.6 1.4 nm on specimen axes Total_Shift = 2.490695 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.32 microns changed by -2.08 to target drift = 0.15 nm/sec Measured defocus = -2.46 microns changed by 0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0644.tif 27 frames are being saved to U:\krios\20190717\PilA_0645.tif 27 frames are being saved to U:\krios\20190717\PilA_0646.tif 27 frames are being saved to U:\krios\20190717\PilA_0647.tif 27 frames are being saved to U:\krios\20190717\PilA_0648.tif 27 frames are being saved to U:\krios\20190717\PilA_0649.tif Running script 17 at item 158 (197.02, -273.82) Remaining time to fill dewars is 720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 100 20 20 70, reference by 219 20 20 240 Disparity in stage position after aligning to target position: 0.29 -0.03 (2nd move error 0.24 0.16) Finished aligning to item in 1 rounds Trimming A by 100 20 25 70, reference by 100 20 20 70 0.9 2.2 unbinned pixels; 6.8 6.5 nm along two shift axes; -8.6 3.0 nm on specimen axes Total_Shift = 4.664044 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.50 microns changed by -2.00 to target drift = 0.17 nm/sec Measured defocus = -2.45 microns changed by -0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0650.tif 27 frames are being saved to U:\krios\20190717\PilA_0651.tif 27 frames are being saved to U:\krios\20190717\PilA_0652.tif 27 frames are being saved to U:\krios\20190717\PilA_0653.tif 27 frames are being saved to U:\krios\20190717\PilA_0654.tif 27 frames are being saved to U:\krios\20190717\PilA_0655.tif Running script 17 at item 159 (199.56, -274.92) Remaining time to fill dewars is 540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 75 24 55 20, reference by 245 22 160 20 Disparity in stage position after aligning to target position: 0.13 -0.47 (2nd move error 0.13 -0.14) Finished aligning to item in 1 rounds Trimming A by 75 24 55 20, reference by 75 24 55 20 -14.2 -1.3 unbinned pixels; 13.7 53.0 nm along two shift axes; 8.1 -54.8 nm on specimen axes Total_Shift = 28.457315 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.54 microns changed by -2.06 to target drift = 0.17 nm/sec Measured defocus = -2.59 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0656.tif 27 frames are being saved to U:\krios\20190717\PilA_0657.tif 27 frames are being saved to U:\krios\20190717\PilA_0658.tif 27 frames are being saved to U:\krios\20190717\PilA_0659.tif 27 frames are being saved to U:\krios\20190717\PilA_0660.tif 27 frames are being saved to U:\krios\20190717\PilA_0661.tif Running script 17 at item 160 (202.02, -276.56) Remaining time to fill dewars is 300 Dewars are NOT busy filling Trimming A by 265 20 165 20, reference by 219 20 20 240 Trimming A by 265 20 165 20, reference by 245 22 160 20 Trimming A by 265 20 165 20, reference by 23 90 20 135 Trimming A by 265 20 165 20, reference by 23 95 90 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.28 Trimming A by 70 24 60 20, reference by 245 22 160 20 Disparity in stage position after aligning to target position: 0.17 -0.44 (2nd move error 0.12 -0.19) Finished aligning to item in 2 rounds Trimming A by 70 24 60 20, reference by 70 24 60 20 -18.0 -4.8 unbinned pixels; 5.7 72.0 nm along two shift axes; 22.6 -68.9 nm on specimen axes Total_Shift = 37.238591 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.84 microns changed by -2.14 to target drift = 0.19 nm/sec Measured defocus = -1.33 microns changed by 0.03 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0662.tif 27 frames are being saved to U:\krios\20190717\PilA_0663.tif 27 frames are being saved to U:\krios\20190717\PilA_0664.tif 27 frames are being saved to U:\krios\20190717\PilA_0665.tif 27 frames are being saved to U:\krios\20190717\PilA_0666.tif 27 frames are being saved to U:\krios\20190717\PilA_0667.tif Running script 17 at item 161 (204.77, -278.34) Remaining time to fill dewars is 180 Dewars are NOT busy filling Trimming A by 21 81 110 40, reference by 219 20 20 240 Trimming A by 21 81 110 40, reference by 245 22 160 20 Trimming A by 21 81 110 40, reference by 23 90 20 135 Trimming A by 21 81 110 40, reference by 23 95 90 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.38 Trimming A by 60 20 75 20, reference by 23 95 90 20 Disparity in stage position after aligning to target position: 0.01 -0.37 (2nd move error -0.05 -0.02) Finished aligning to item in 2 rounds Trimming A by 60 20 75 20, reference by 60 20 75 20 -2.8 9.6 unbinned pixels; 39.1 4.3 nm along two shift axes; -36.9 -13.0 nm on specimen axes Total_Shift = 20.087263 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.55 microns changed by -2.95 to target drift = 1.87 nm/sec Measured defocus = -1.95 microns changed by 0.55 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0668.tif 27 frames are being saved to U:\krios\20190717\PilA_0669.tif 27 frames are being saved to U:\krios\20190717\PilA_0670.tif 27 frames are being saved to U:\krios\20190717\PilA_0671.tif 27 frames are being saved to U:\krios\20190717\PilA_0672.tif 27 frames are being saved to U:\krios\20190717\PilA_0673.tif Running script 17 at item 162 (205.63, -282.70) Remaining time to fill dewars is 540 Dewars ARE busy filling Dewars ARE busy filling Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 105 125 20, reference by 23 95 90 20 Disparity in stage position after aligning to target position: -0.01 -0.29 (2nd move error -0.07 0.07) Finished aligning to item in 1 rounds Trimming A by 24 105 120 20, reference by 24 105 125 20 1.2 19.7 unbinned pixels; 71.0 33.9 nm along two shift axes; -77.0 0.4 nm on specimen axes Total_Shift = 39.52883 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.75 microns changed by -2.25 to target drift = 0.16 nm/sec Measured defocus = -1.54 microns changed by 0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0674.tif 27 frames are being saved to U:\krios\20190717\PilA_0675.tif 27 frames are being saved to U:\krios\20190717\PilA_0676.tif 27 frames are being saved to U:\krios\20190717\PilA_0677.tif 27 frames are being saved to U:\krios\20190717\PilA_0678.tif 27 frames are being saved to U:\krios\20190717\PilA_0679.tif Running script 17 at item 163 (203.13, -280.98) Remaining time to fill dewars is 31560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 45 45 20, reference by 23 95 90 20 Disparity in stage position after aligning to target position: -0.02 -0.46 (2nd move error -0.07 -0.10) Finished aligning to item in 1 rounds Trimming A by 21 45 45 20, reference by 21 45 45 20 -9.1 14.5 unbinned pixels; 65.3 10.9 nm along two shift axes; -54.6 -38.4 nm on specimen axes Total_Shift = 34.266543 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.60 microns changed by -2.20 to target drift = 0.06 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0680.tif 27 frames are being saved to U:\krios\20190717\PilA_0681.tif 27 frames are being saved to U:\krios\20190717\PilA_0682.tif 27 frames are being saved to U:\krios\20190717\PilA_0683.tif 27 frames are being saved to U:\krios\20190717\PilA_0684.tif 27 frames are being saved to U:\krios\20190717\PilA_0685.tif Running script 17 at item 164 (195.26, -276.38) Remaining time to fill dewars is 31440 Dewars are NOT busy filling Trimming A by 250 24 20 240, reference by 219 20 20 240 Trimming A by 250 24 20 240, reference by 245 22 160 20 Trimming A by 250 24 20 240, reference by 23 90 20 135 Trimming A by 250 24 20 240, reference by 23 95 90 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.25 Trimming A by 71 20 20 95, reference by 219 20 20 240 Disparity in stage position after aligning to target position: 0.31 -0.12 (2nd move error 0.26 0.10) Finished aligning to item in 2 rounds Trimming A by 80 24 20 80, reference by 71 20 20 95 1.2 -0.2 unbinned pixels; 2.1 3.9 nm along two shift axes; 0.4 4.6 nm on specimen axes Total_Shift = 2.347356 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.41 microns changed by -2.11 to target drift = 0.14 nm/sec Measured defocus = -1.72 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0686.tif 27 frames are being saved to U:\krios\20190717\PilA_0687.tif 27 frames are being saved to U:\krios\20190717\PilA_0688.tif 27 frames are being saved to U:\krios\20190717\PilA_0689.tif 27 frames are being saved to U:\krios\20190717\PilA_0690.tif 27 frames are being saved to U:\krios\20190717\PilA_0691.tif Running script 17 at item 165 (192.88, -274.70) Remaining time to fill dewars is 31380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 136 23 20 165, reference by 219 20 20 240 Disparity in stage position after aligning to target position: 0.26 -0.24 (2nd move error 0.22 -0.01) Finished aligning to item in 1 rounds Trimming A by 145 21 20 150, reference by 136 23 20 165 0.8 -3.4 unbinned pixels; 13.6 2.5 nm along two shift axes; 13.2 3.7 nm on specimen axes Total_Shift = 7.036152 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.16 microns changed by -1.96 to target drift = 0.18 nm/sec Measured defocus = -1.75 microns changed by -0.05 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0692.tif 27 frames are being saved to U:\krios\20190717\PilA_0693.tif 27 frames are being saved to U:\krios\20190717\PilA_0694.tif 27 frames are being saved to U:\krios\20190717\PilA_0695.tif 27 frames are being saved to U:\krios\20190717\PilA_0696.tif 27 frames are being saved to U:\krios\20190717\PilA_0697.tif Running script 17 at item 166 (193.99, -278.91) Remaining time to fill dewars is 31260 Dewars are NOT busy filling Trimming A by 21 82 20 155, reference by 219 20 20 240 Trimming A by 21 82 20 155, reference by 245 22 160 20 Trimming A by 21 82 20 155, reference by 23 90 20 135 Trimming A by 21 82 20 155, reference by 23 95 90 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.30 Trimming A by 43 23 20 105, reference by 23 90 20 135 Disparity in stage position after aligning to target position: 0.07 -0.18 (2nd move error 0.01 0.09) Finished aligning to item in 2 rounds Trimming A by 52 23 20 100, reference by 43 23 20 105 -3.5 0.5 unbinned pixels; 6.4 11.9 nm along two shift axes; -1.2 -13.7 nm on specimen axes Total_Shift = 7.078872 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.13 microns changed by -2.03 to target drift = 0.09 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0698.tif 27 frames are being saved to U:\krios\20190717\PilA_0699.tif 27 frames are being saved to U:\krios\20190717\PilA_0700.tif 27 frames are being saved to U:\krios\20190717\PilA_0701.tif 27 frames are being saved to U:\krios\20190717\PilA_0702.tif 27 frames are being saved to U:\krios\20190717\PilA_0703.tif Running script 17 at item 167 (196.48, -280.67) Remaining time to fill dewars is 31020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 90 20 135 Disparity in stage position after aligning to target position: 0.10 -0.11 (2nd move error 0.05 0.17) Finished aligning to item in 1 rounds -0.1 -1.5 unbinned pixels; 5.3 2.7 nm along two shift axes; 5.8 -0.2 nm on specimen axes Total_Shift = 2.970008 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = -0.05 microns changed by -1.95 to target drift = 0.14 nm/sec Measured defocus = -1.96 microns changed by -0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0704.tif 27 frames are being saved to U:\krios\20190717\PilA_0705.tif 27 frames are being saved to U:\krios\20190717\PilA_0706.tif 27 frames are being saved to U:\krios\20190717\PilA_0707.tif 27 frames are being saved to U:\krios\20190717\PilA_0708.tif 27 frames are being saved to U:\krios\20190717\PilA_0709.tif Running script 17 at item 168 (199.01, -281.78) Remaining time to fill dewars is 31020 Dewars are NOT busy filling Trimming A by 20 86 110 45, reference by 219 20 20 240 Trimming A by 20 86 110 45, reference by 245 22 160 20 Trimming A by 20 86 110 45, reference by 23 90 20 135 Trimming A by 20 86 110 45, reference by 23 95 90 20 Disparity in stage position after aligning to center of map frame: 0.02 -0.31 Trimming A by 20 20 20 20, reference by 23 95 90 20 Disparity in stage position after aligning to target position: -0.00 -0.60 (2nd move error -0.03 -0.31) Finished aligning to item in 2 rounds -29.8 6.0 unbinned pixels; 60.8 98.1 nm along two shift axes; -17.0 -117.0 nm on specimen axes Total_Shift = 60.702673 1.3 3.3 unbinned pixels; 10.6 9.6 nm along two shift axes; -13.3 4.2 nm on specimen axes Total_Shift = 7.148745 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = -0.01 microns changed by -2.09 to target drift = 0.04 nm/sec Measured defocus = -2.12 microns changed by 0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0710.tif 27 frames are being saved to U:\krios\20190717\PilA_0711.tif 27 frames are being saved to U:\krios\20190717\PilA_0712.tif 27 frames are being saved to U:\krios\20190717\PilA_0713.tif 27 frames are being saved to U:\krios\20190717\PilA_0714.tif 27 frames are being saved to U:\krios\20190717\PilA_0715.tif Running script 17 at item 169 (201.50, -283.54) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 50 45 20, reference by 23 95 90 20 Disparity in stage position after aligning to target position: 0.02 -0.47 (2nd move error -0.01 -0.18) Finished aligning to item in 1 rounds Trimming A by 20 50 45 20, reference by 20 50 45 20 -22.7 4.0 unbinned pixels; 44.4 75.7 nm along two shift axes; -10.8 -89.2 nm on specimen axes Total_Shift = 46.108194 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.06 microns changed by -2.14 to target drift = 0.09 nm/sec Measured defocus = -2.26 microns changed by 0.06 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0716.tif 27 frames are being saved to U:\krios\20190717\PilA_0717.tif 27 frames are being saved to U:\krios\20190717\PilA_0718.tif 27 frames are being saved to U:\krios\20190717\PilA_0719.tif 27 frames are being saved to U:\krios\20190717\PilA_0720.tif 27 frames are being saved to U:\krios\20190717\PilA_0721.tif Running script 17 at item 170 (199.92, -286.12) Remaining time to fill dewars is 30720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 85 25 65, reference by 23 95 90 20 Disparity in stage position after aligning to target position: 0.06 -0.56 (2nd move error 0.04 -0.27) Finished aligning to item in 1 rounds Trimming A by 24 90 25 65, reference by 20 85 25 65 -28.5 -4.6 unbinned pixels; 20.4 109.5 nm along two shift axes; 23.7 -109.9 nm on specimen axes Total_Shift = 57.722544 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.20 microns changed by -2.10 to target drift = 0.25 nm/sec Measured defocus = -2.36 microns changed by 0.06 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0722.tif 27 frames are being saved to U:\krios\20190717\PilA_0723.tif 27 frames are being saved to U:\krios\20190717\PilA_0724.tif 27 frames are being saved to U:\krios\20190717\PilA_0725.tif 27 frames are being saved to U:\krios\20190717\PilA_0726.tif 27 frames are being saved to U:\krios\20190717\PilA_0727.tif Running script 17 at item 171 (197.38, -284.96) Remaining time to fill dewars is 30660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 75 20 75, reference by 23 90 20 135 Disparity in stage position after aligning to target position: 0.15 -0.08 (2nd move error 0.09 0.19) Finished aligning to item in 1 rounds Trimming A by 20 70 20 70, reference by 20 75 20 75 2.9 1.9 unbinned pixels; 3.4 13.3 nm along two shift axes; -8.2 10.8 nm on specimen axes Total_Shift = 6.942696 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.38 microns changed by -2.02 to target drift = 0.13 nm/sec Measured defocus = -2.41 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0728.tif 27 frames are being saved to U:\krios\20190717\PilA_0729.tif 27 frames are being saved to U:\krios\20190717\PilA_0730.tif 27 frames are being saved to U:\krios\20190717\PilA_0731.tif 27 frames are being saved to U:\krios\20190717\PilA_0732.tif 27 frames are being saved to U:\krios\20190717\PilA_0733.tif Running script 17 at item 172 (194.88, -283.23) Remaining time to fill dewars is 30540 Dewars are NOT busy filling Trimming A by 21 82 20 155, reference by 219 20 20 240 Trimming A by 21 82 20 155, reference by 245 22 160 20 Trimming A by 21 82 20 155, reference by 23 90 20 135 Trimming A by 21 82 20 155, reference by 23 95 90 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.29 Trimming A by 21 70 20 80, reference by 23 90 20 135 Disparity in stage position after aligning to target position: 0.09 -0.12 (2nd move error 0.07 0.15) Finished aligning to item in 2 rounds Trimming A by 20 65 20 75, reference by 21 70 20 80 -3.8 1.1 unbinned pixels; 9.1 12.0 nm along two shift axes; -3.6 -15.1 nm on specimen axes Total_Shift = 7.955942 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.51 microns changed by -1.99 to target drift = 0.15 nm/sec Measured defocus = -2.49 microns changed by -0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0734.tif 27 frames are being saved to U:\krios\20190717\PilA_0735.tif 27 frames are being saved to U:\krios\20190717\PilA_0736.tif 27 frames are being saved to U:\krios\20190717\PilA_0737.tif 27 frames are being saved to U:\krios\20190717\PilA_0738.tif 27 frames are being saved to U:\krios\20190717\PilA_0739.tif Running script 17 at item 173 (192.35, -281.43) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 45 45 20 110, reference by 23 90 20 135 Disparity in stage position after aligning to target position: 0.04 -0.26 (2nd move error 0.02 0.00) Finished aligning to item in 1 rounds Trimming A by 45 45 20 110, reference by 45 45 20 110 0.5 -2.0 unbinned pixels; 7.9 1.1 nm along two shift axes; 7.6 2.4 nm on specimen axes Total_Shift = 4.083552 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.73 microns changed by -1.87 to target drift = 0.11 nm/sec Measured defocus = -2.53 microns changed by -0.07 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0740.tif 27 frames are being saved to U:\krios\20190717\PilA_0741.tif 27 frames are being saved to U:\krios\20190717\PilA_0742.tif 27 frames are being saved to U:\krios\20190717\PilA_0743.tif 27 frames are being saved to U:\krios\20190717\PilA_0744.tif 27 frames are being saved to U:\krios\20190717\PilA_0745.tif Running script 17 at item 174 (193.17, -285.78) Remaining time to fill dewars is 30240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 140 20 145, reference by 23 90 20 135 Disparity in stage position after aligning to target position: 0.17 -0.21 (2nd move error 0.15 0.05) Finished aligning to item in 1 rounds Trimming A by 24 135 20 140, reference by 22 140 20 145 -0.2 -1.0 unbinned pixels; 3.6 2.1 nm along two shift axes; 4.1 -0.4 nm on specimen axes Total_Shift = 2.101579 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.62 microns changed by -1.92 to target drift = 0.17 nm/sec Measured defocus = -1.23 microns changed by -0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0746.tif 27 frames are being saved to U:\krios\20190717\PilA_0747.tif 27 frames are being saved to U:\krios\20190717\PilA_0748.tif 27 frames are being saved to U:\krios\20190717\PilA_0749.tif 27 frames are being saved to U:\krios\20190717\PilA_0750.tif 27 frames are being saved to U:\krios\20190717\PilA_0751.tif Running script 17 at item 175 (195.67, -287.45) Remaining time to fill dewars is 30240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 150 20 135, reference by 23 90 20 135 Disparity in stage position after aligning to target position: 0.17 -0.08 (2nd move error 0.14 0.19) Finished aligning to item in 1 rounds Trimming A by 22 145 20 130, reference by 20 150 20 135 0.2 -0.4 unbinned pixels; 2.0 0.2 nm along two shift axes; 1.7 1.0 nm on specimen axes Total_Shift = 1.011188 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.58 microns changed by -1.98 to target drift = 0.16 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0752.tif 27 frames are being saved to U:\krios\20190717\PilA_0753.tif 27 frames are being saved to U:\krios\20190717\PilA_0754.tif 27 frames are being saved to U:\krios\20190717\PilA_0755.tif 27 frames are being saved to U:\krios\20190717\PilA_0756.tif 27 frames are being saved to U:\krios\20190717\PilA_0757.tif Running script 17 at item 176 (343.92, -284.42) Remaining time to fill dewars is 30000 Dewars are NOT busy filling Trimming A by 229 24 195 20, reference by 210 21 20 210 Trimming A by 229 24 195 20, reference by 210 20 175 20 Trimming A by 229 24 195 20, reference by 24 170 20 185 Trimming A by 229 24 195 20, reference by 24 175 160 20 Disparity in stage position after aligning to center of map frame: -0.17 -0.33 Trimming A by 51 46 190 20, reference by 210 20 175 20 Disparity in stage position after aligning to target position: 0.05 -0.25 (2nd move error 0.21 0.04) Finished aligning to item in 2 rounds Trimming A by 59 49 195 20, reference by 51 46 190 20 -1.2 6.4 unbinned pixels; 25.1 5.2 nm along two shift axes; -24.6 -6.1 nm on specimen axes Total_Shift = 13.017214 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.41 microns changed by -3.91 to target drift = 0.24 nm/sec Measured defocus = -1.51 microns changed by 0.01 to target drift = 0.16 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0758.tif 27 frames are being saved to U:\krios\20190717\PilA_0759.tif 27 frames are being saved to U:\krios\20190717\PilA_0760.tif 27 frames are being saved to U:\krios\20190717\PilA_0761.tif 27 frames are being saved to U:\krios\20190717\PilA_0762.tif 27 frames are being saved to U:\krios\20190717\PilA_0763.tif Running script 17 at item 177 (341.43, -282.62) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 105 20 125 20, reference by 210 20 175 20 Disparity in stage position after aligning to target position: -0.03 -0.42 (2nd move error 0.13 -0.12) Finished aligning to item in 1 rounds Trimming A by 105 20 125 20, reference by 105 20 125 20 -14.0 -0.8 unbinned pixels; 15.3 51.6 nm along two shift axes; 6.1 -54.2 nm on specimen axes Total_Shift = 28.019192 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.88 microns changed by -2.48 to target drift = 0.13 nm/sec Measured defocus = -1.57 microns changed by -0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0764.tif 27 frames are being saved to U:\krios\20190717\PilA_0765.tif 27 frames are being saved to U:\krios\20190717\PilA_0766.tif 27 frames are being saved to U:\krios\20190717\PilA_0767.tif 27 frames are being saved to U:\krios\20190717\PilA_0768.tif 27 frames are being saved to U:\krios\20190717\PilA_0769.tif Running script 17 at item 178 (338.93, -280.91) Remaining time to fill dewars is 29820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 120 20 100 20, reference by 210 20 175 20 Disparity in stage position after aligning to target position: -0.03 -0.54 (2nd move error 0.13 -0.22) Finished aligning to item in 1 rounds Trimming A by 115 22 100 20, reference by 120 20 100 20 -21.6 -9.4 unbinned pixels; 6.5 92.0 nm along two shift axes; 41.2 -82.1 nm on specimen axes Total_Shift = 47.148619 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 2.05 microns changed by -3.75 to target drift = 0.17 nm/sec Measured defocus = -1.77 microns changed by 0.07 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0770.tif 27 frames are being saved to U:\krios\20190717\PilA_0771.tif 27 frames are being saved to U:\krios\20190717\PilA_0772.tif 27 frames are being saved to U:\krios\20190717\PilA_0773.tif 27 frames are being saved to U:\krios\20190717\PilA_0774.tif 27 frames are being saved to U:\krios\20190717\PilA_0775.tif Running script 17 at item 179 (336.47, -279.25) Remaining time to fill dewars is 29580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 24 80 25, reference by 210 20 175 20 Disparity in stage position after aligning to target position: 0.00 -0.60 (2nd move error 0.17 -0.29) Finished aligning to item in 1 rounds Trimming A by 135 23 80 20, reference by 135 24 80 25 -27.8 -6.9 unbinned pixels; 11.1 110.5 nm along two shift axes; 32.6 -106.8 nm on specimen axes Total_Shift = 57.295737 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.47 microns changed by -2.27 to target drift = 0.22 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0776.tif 27 frames are being saved to U:\krios\20190717\PilA_0777.tif 27 frames are being saved to U:\krios\20190717\PilA_0778.tif 27 frames are being saved to U:\krios\20190717\PilA_0779.tif 27 frames are being saved to U:\krios\20190717\PilA_0780.tif 27 frames are being saved to U:\krios\20190717\PilA_0781.tif Running script 17 at item 180 (333.98, -278.05) Remaining time to fill dewars is 29520 Dewars are NOT busy filling Trimming A by 230 21 20 220, reference by 210 21 20 210 Trimming A by 230 21 20 220, reference by 210 20 175 20 Trimming A by 230 21 20 220, reference by 24 170 20 185 Trimming A by 230 21 20 220, reference by 24 175 160 20 Disparity in stage position after aligning to center of map frame: -0.01 -0.29 Trimming A by 170 20 20 105, reference by 210 21 20 210 Disparity in stage position after aligning to target position: -0.02 -0.04 (2nd move error -0.01 0.23) Finished aligning to item in 2 rounds Trimming A by 180 20 20 100, reference by 170 20 20 105 -1.6 8.0 unbinned pixels; 31.6 6.4 nm along two shift axes; -30.9 -7.8 nm on specimen axes Total_Shift = 16.35492 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.63 microns changed by -3.53 to target drift = 0.15 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0782.tif 27 frames are being saved to U:\krios\20190717\PilA_0783.tif 27 frames are being saved to U:\krios\20190717\PilA_0784.tif 27 frames are being saved to U:\krios\20190717\PilA_0785.tif 27 frames are being saved to U:\krios\20190717\PilA_0786.tif 27 frames are being saved to U:\krios\20190717\PilA_0787.tif Running script 17 at item 181 (332.35, -280.67) Remaining time to fill dewars is 29400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 22 20 120, reference by 210 21 20 210 Disparity in stage position after aligning to target position: 0.06 -0.21 (2nd move error 0.07 0.06) Finished aligning to item in 1 rounds Trimming A by 135 22 20 120, reference by 135 22 20 120 -1.7 -1.4 unbinned pixels; 2.8 8.1 nm along two shift axes; 5.6 -6.2 nm on specimen axes Total_Shift = 4.320381 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.20 microns changed by -2.20 to target drift = 0.16 nm/sec Measured defocus = -2.03 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0788.tif 27 frames are being saved to U:\krios\20190717\PilA_0789.tif 27 frames are being saved to U:\krios\20190717\PilA_0790.tif 27 frames are being saved to U:\krios\20190717\PilA_0791.tif 27 frames are being saved to U:\krios\20190717\PilA_0792.tif 27 frames are being saved to U:\krios\20190717\PilA_0793.tif Running script 17 at item 182 (334.83, -282.09) Remaining time to fill dewars is 29160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 20 20 55, reference by 210 21 20 210 Disparity in stage position after aligning to target position: 0.05 -0.02 (2nd move error 0.06 0.25) Finished aligning to item in 1 rounds Trimming A by 75 20 20 55, reference by 70 20 20 55 -2.3 1.7 unbinned pixels; 9.2 5.5 nm along two shift axes; -6.2 -9.1 nm on specimen axes Total_Shift = 5.648712 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.35 microns changed by -3.45 to target drift = 0.12 nm/sec Measured defocus = -2.07 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0794.tif 27 frames are being saved to U:\krios\20190717\PilA_0795.tif 27 frames are being saved to U:\krios\20190717\PilA_0796.tif 27 frames are being saved to U:\krios\20190717\PilA_0797.tif 27 frames are being saved to U:\krios\20190717\PilA_0798.tif 27 frames are being saved to U:\krios\20190717\PilA_0799.tif Running script 17 at item 183 (337.37, -283.45) Remaining time to fill dewars is 29100 Dewars are NOT busy filling Trimming A by 229 22 190 20, reference by 210 21 20 210 Trimming A by 229 22 190 20, reference by 210 20 175 20 Trimming A by 229 22 190 20, reference by 24 170 20 185 Trimming A by 229 22 190 20, reference by 24 175 160 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.29 Trimming A by 45 21 40 20, reference by 210 20 175 20 Disparity in stage position after aligning to target position: 0.06 -0.57 (2nd move error 0.14 -0.30) Finished aligning to item in 2 rounds Trimming A by 45 20 35 20, reference by 45 21 40 20 -24.6 -1.0 unbinned pixels; 28.5 90.0 nm along two shift axes; 9.0 -95.4 nm on specimen axes Total_Shift = 49.192653 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.12 microns changed by -2.32 to target drift = 0.20 nm/sec Measured defocus = -2.17 microns changed by -0.03 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0800.tif 27 frames are being saved to U:\krios\20190717\PilA_0801.tif 27 frames are being saved to U:\krios\20190717\PilA_0802.tif 27 frames are being saved to U:\krios\20190717\PilA_0803.tif 27 frames are being saved to U:\krios\20190717\PilA_0804.tif 27 frames are being saved to U:\krios\20190717\PilA_0805.tif Running script 17 at item 184 (340.11, -285.16) Remaining time to fill dewars is 28980 Dewars are NOT busy filling Trimming A by 24 180 170 20, reference by 210 21 20 210 Trimming A by 24 180 170 20, reference by 210 20 175 20 Trimming A by 24 180 170 20, reference by 24 170 20 185 Trimming A by 24 180 170 20, reference by 24 175 160 20 Disparity in stage position after aligning to center of map frame: -0.07 -0.35 Trimming A by 27 40 75 20, reference by 24 175 160 20 Disparity in stage position after aligning to target position: -0.11 -0.52 (2nd move error -0.05 -0.20) Finished aligning to item in 2 rounds Trimming A by 24 27 90 20, reference by 27 40 75 20 -22.9 31.1 unbinned pixels; 144.2 35.6 nm along two shift axes; -116.1 -95.6 nm on specimen axes Total_Shift = 77.213863 Trimming A by 22 40 80 20, reference by 27 40 75 20 -1.2 19.8 unbinned pixels; 74.2 25.5 nm along two shift axes; -76.7 -8.7 nm on specimen axes Total_Shift = 39.613045 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.42 microns changed by -3.72 to target drift = 0.02 nm/sec Measured defocus = -2.36 microns changed by 0.06 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0806.tif 27 frames are being saved to U:\krios\20190717\PilA_0807.tif 27 frames are being saved to U:\krios\20190717\PilA_0808.tif 27 frames are being saved to U:\krios\20190717\PilA_0809.tif 27 frames are being saved to U:\krios\20190717\PilA_0810.tif 27 frames are being saved to U:\krios\20190717\PilA_0811.tif Running script 17 at item 185 (342.58, -286.95) Remaining time to fill dewars is 28800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 92 160 20, reference by 24 175 160 20 Disparity in stage position after aligning to target position: -0.08 -0.39 (2nd move error -0.03 -0.07) Finished aligning to item in 1 rounds Trimming A by 20 96 160 20, reference by 23 92 160 20 -10.2 21.3 unbinned pixels; 91.6 4.8 nm along two shift axes; -80.6 -44.3 nm on specimen axes Total_Shift = 47.235043 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.09 microns changed by -2.49 to target drift = 0.07 nm/sec Measured defocus = -2.48 microns changed by 0.08 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0812.tif 27 frames are being saved to U:\krios\20190717\PilA_0813.tif 27 frames are being saved to U:\krios\20190717\PilA_0814.tif 27 frames are being saved to U:\krios\20190717\PilA_0815.tif 27 frames are being saved to U:\krios\20190717\PilA_0816.tif 27 frames are being saved to U:\krios\20190717\PilA_0817.tif Running script 17 at item 186 (341.00, -289.46) Remaining time to fill dewars is 28800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 116 140 20, reference by 24 175 160 20 Disparity in stage position after aligning to target position: -0.09 -0.50 (2nd move error -0.03 -0.17) Finished aligning to item in 1 rounds Trimming A by 21 135 125 20, reference by 24 116 140 20 -18.1 5.7 unbinned pixels; 44.6 56.7 nm along two shift axes; -18.3 -71.7 nm on specimen axes Total_Shift = 37.983028 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.28 microns changed by -3.78 to target drift = 0.03 nm/sec Measured defocus = -2.59 microns changed by 0.09 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0818.tif 27 frames are being saved to U:\krios\20190717\PilA_0819.tif 27 frames are being saved to U:\krios\20190717\PilA_0820.tif 27 frames are being saved to U:\krios\20190717\PilA_0821.tif 27 frames are being saved to U:\krios\20190717\PilA_0822.tif 27 frames are being saved to U:\krios\20190717\PilA_0823.tif Running script 17 at item 187 (338.42, -287.69) Remaining time to fill dewars is 28560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 60 60 20, reference by 24 175 160 20 Disparity in stage position after aligning to target position: -0.25 -0.54 (2nd move error -0.19 -0.22) Finished aligning to item in 1 rounds Trimming A by 24 51 70 20, reference by 20 60 60 20 -22.7 36.3 unbinned pixels; 163.0 27.1 nm along two shift axes; -136.3 -95.9 nm on specimen axes Total_Shift = 85.57841 Trimming A by 24 51 70 20, reference by 20 60 60 20 -1.9 22.2 unbinned pixels; 84.0 26.4 nm along two shift axes; -85.8 -12.2 nm on specimen axes Total_Shift = 44.468982 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.27 microns changed by -2.33 to target drift = 0.05 nm/sec Measured defocus = -2.61 microns changed by 0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0824.tif 27 frames are being saved to U:\krios\20190717\PilA_0825.tif 27 frames are being saved to U:\krios\20190717\PilA_0826.tif 27 frames are being saved to U:\krios\20190717\PilA_0827.tif 27 frames are being saved to U:\krios\20190717\PilA_0828.tif 27 frames are being saved to U:\krios\20190717\PilA_0829.tif Running script 17 at item 188 (336.05, -285.99) Remaining time to fill dewars is 28440 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 175 160 20 Disparity in stage position after aligning to target position: -0.16 -0.71 (2nd move error -0.10 -0.39) Finished aligning to item in 1 rounds -32.6 20.5 unbinned pixels; 117.9 86.7 nm along two shift axes; -72.8 -131.3 nm on specimen axes Total_Shift = 77.081496 -0.7 13.3 unbinned pixels; 49.7 17.6 nm along two shift axes; -51.6 -5.4 nm on specimen axes Total_Shift = 26.621156 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.03 microns changed by -2.33 to target drift = 0.03 nm/sec Measured defocus = -1.30 microns changed by -0.00 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0830.tif 27 frames are being saved to U:\krios\20190717\PilA_0831.tif 27 frames are being saved to U:\krios\20190717\PilA_0832.tif 27 frames are being saved to U:\krios\20190717\PilA_0833.tif 27 frames are being saved to U:\krios\20190717\PilA_0834.tif 27 frames are being saved to U:\krios\20190717\PilA_0835.tif Running script 17 at item 189 (333.24, -284.74) Remaining time to fill dewars is 28260 Dewars are NOT busy filling Trimming A by 230 21 20 220, reference by 210 21 20 210 Trimming A by 230 21 20 220, reference by 210 20 175 20 Trimming A by 230 21 20 220, reference by 24 170 20 185 Trimming A by 230 21 20 220, reference by 24 175 160 20 Disparity in stage position after aligning to center of map frame: -0.03 -0.29 Trimming A by 36 20 20 75, reference by 210 21 20 210 Disparity in stage position after aligning to target position: 0.11 -0.06 (2nd move error 0.13 0.20) Finished aligning to item in 2 rounds Trimming A by 40 20 20 70, reference by 36 20 20 75 3.1 5.1 unbinned pixels; 14.6 18.8 nm along two shift axes; -20.4 10.9 nm on specimen axes Total_Shift = 11.889717 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.21 microns changed by -3.61 to target drift = 0.14 nm/sec Measured defocus = -1.46 microns changed by 0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0836.tif 27 frames are being saved to U:\krios\20190717\PilA_0837.tif 27 frames are being saved to U:\krios\20190717\PilA_0838.tif 27 frames are being saved to U:\krios\20190717\PilA_0839.tif 27 frames are being saved to U:\krios\20190717\PilA_0840.tif 27 frames are being saved to U:\krios\20190717\PilA_0841.tif Running script 17 at item 190 (330.66, -283.21) Remaining time to fill dewars is 28140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 20 20 165, reference by 210 21 20 210 Disparity in stage position after aligning to target position: -0.02 -0.24 (2nd move error 0.00 0.04) Finished aligning to item in 1 rounds Trimming A by 80 21 20 160, reference by 70 20 20 165 -1.8 0.8 unbinned pixels; 5.3 5.3 nm along two shift axes; -2.7 -7.2 nm on specimen axes Total_Shift = 3.945462 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.57 microns changed by -2.07 to target drift = 0.14 nm/sec Measured defocus = -1.45 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0842.tif 27 frames are being saved to U:\krios\20190717\PilA_0843.tif 27 frames are being saved to U:\krios\20190717\PilA_0844.tif 27 frames are being saved to U:\krios\20190717\PilA_0845.tif 27 frames are being saved to U:\krios\20190717\PilA_0846.tif 27 frames are being saved to U:\krios\20190717\PilA_0847.tif Running script 17 at item 191 (329.27, -285.77) Remaining time to fill dewars is 27960 Dewars are NOT busy filling Trimming A by 21 180 50 185, reference by 210 21 20 210 Trimming A by 21 180 50 185, reference by 210 20 175 20 Trimming A by 21 180 50 185, reference by 24 170 20 185 Trimming A by 21 180 50 185, reference by 24 175 160 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.32 Trimming A by 71 25 20 165, reference by 24 170 20 185 Disparity in stage position after aligning to target position: -0.11 -0.24 (2nd move error -0.07 0.05) Finished aligning to item in 2 rounds Trimming A by 59 24 20 175, reference by 71 25 20 165 -0.7 12.6 unbinned pixels; 47.2 16.5 nm along two shift axes; -48.9 -5.3 nm on specimen axes Total_Shift = 25.232762 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.83 microns changed by -3.43 to target drift = 0.20 nm/sec Measured defocus = -1.66 microns changed by 0.06 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0848.tif 27 frames are being saved to U:\krios\20190717\PilA_0849.tif 27 frames are being saved to U:\krios\20190717\PilA_0850.tif 27 frames are being saved to U:\krios\20190717\PilA_0851.tif 27 frames are being saved to U:\krios\20190717\PilA_0852.tif 27 frames are being saved to U:\krios\20190717\PilA_0853.tif Running script 17 at item 192 (331.76, -287.48) Remaining time to fill dewars is 27840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 70 20 105, reference by 24 170 20 185 Disparity in stage position after aligning to target position: 0.03 -0.20 (2nd move error 0.07 0.09) Finished aligning to item in 1 rounds Trimming A by 22 52 20 115, reference by 21 70 20 105 -3.2 -2.3 unbinned pixels; 4.3 14.9 nm along two shift axes; 9.6 -11.9 nm on specimen axes Total_Shift = 7.858088 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.43 microns changed by -2.13 to target drift = 0.12 nm/sec Measured defocus = -1.65 microns changed by -0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0854.tif 27 frames are being saved to U:\krios\20190717\PilA_0855.tif Ending Navigator acquisition after current item 27 frames are being saved to U:\krios\20190717\PilA_0856.tif 27 frames are being saved to U:\krios\20190717\PilA_0857.tif 27 frames are being saved to U:\krios\20190717\PilA_0858.tif 27 frames are being saved to U:\krios\20190717\PilA_0859.tif Running script 17 at item 193 (334.30, -289.15) Remaining time to fill dewars is 27660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 85 20 85, reference by 24 170 20 185 Disparity in stage position after aligning to target position: 0.05 -0.12 (2nd move error 0.09 0.18) Finished aligning to item in 1 rounds Trimming A by 20 80 20 80, reference by 20 85 20 85 1.2 -1.2 unbinned pixels; 6.0 2.3 nm along two shift axes; 4.5 4.8 nm on specimen axes Total_Shift = 3.378345 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.71 microns changed by -3.51 to target drift = 0.15 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0860.tif 27 frames are being saved to U:\krios\20190717\PilA_0861.tif 27 frames are being saved to U:\krios\20190717\PilA_0862.tif 27 frames are being saved to U:\krios\20190717\PilA_0863.tif 27 frames are being saved to U:\krios\20190717\PilA_0864.tif 27 frames are being saved to U:\krios\20190717\PilA_0865.tif Running script 17 at item 194 (336.84, -290.33) Remaining time to fill dewars is 27540 Dewars are NOT busy filling Trimming A by 24 185 170 20, reference by 210 21 20 210 Trimming A by 24 185 170 20, reference by 210 20 175 20 Trimming A by 24 185 170 20, reference by 24 170 20 185 Trimming A by 24 185 170 20, reference by 24 175 160 20 Disparity in stage position after aligning to center of map frame: -0.10 -0.31 Trimming A by 21 95 55 60, reference by 24 175 160 20 Disparity in stage position after aligning to target position: -0.08 -0.63 (2nd move error 0.01 -0.35) Finished aligning to item in 2 rounds Trimming A by 20 100 40 65, reference by 21 95 55 60 -32.4 1.5 unbinned pixels; 47.9 114.5 nm along two shift axes; 1.0 -126.5 nm on specimen axes Total_Shift = 64.965828 Trimming A by 21 105 60 65, reference by 21 95 55 60 -0.3 -0.8 unbinned pixels; 2.3 2.4 nm along two shift axes; 3.0 -1.2 nm on specimen axes Total_Shift = 1.661241 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.41 microns changed by -2.31 to target drift = 0.11 nm/sec Measured defocus = -1.87 microns changed by -0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0866.tif 27 frames are being saved to U:\krios\20190717\PilA_0867.tif 27 frames are being saved to U:\krios\20190717\PilA_0868.tif 27 frames are being saved to U:\krios\20190717\PilA_0869.tif 27 frames are being saved to U:\krios\20190717\PilA_0870.tif 27 frames are being saved to U:\krios\20190717\PilA_0871.tif Running script 17 at item 195 (339.31, -292.02) Remaining time to fill dewars is 27300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 160 105 20, reference by 24 175 160 20 Disparity in stage position after aligning to target position: -0.00 -0.53 (2nd move error 0.08 -0.25) Finished aligning to item in 1 rounds Trimming A by 21 160 110 20, reference by 21 160 105 20 -28.0 -7.8 unbinned pixels; 7.8 112.7 nm along two shift axes; 36.4 -107.5 nm on specimen axes Total_Shift = 58.234646 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.69 microns changed by -3.69 to target drift = 0.20 nm/sec Measured defocus = -2.04 microns changed by 0.04 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0872.tif 27 frames are being saved to U:\krios\20190717\PilA_0873.tif 27 frames are being saved to U:\krios\20190717\PilA_0874.tif 27 frames are being saved to U:\krios\20190717\PilA_0875.tif 27 frames are being saved to U:\krios\20190717\PilA_0876.tif 27 frames are being saved to U:\krios\20190717\PilA_0877.tif Running script 17 at item 196 (372.25, -226.14) Remaining time to fill dewars is 27120 Dewars are NOT busy filling Trimming A by 225 23 185 20, reference by 210 23 20 225 Trimming A by 225 23 185 20, reference by 200 21 170 20 Trimming A by 225 23 185 20, reference by 20 205 20 220 Trimming A by 225 23 185 20, reference by 21 200 170 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.36 Trimming A by 135 20 165 20, reference by 200 21 170 20 Disparity in stage position after aligning to target position: -0.04 -0.32 (2nd move error 0.09 0.02) Finished aligning to item in 2 rounds Trimming A by 140 20 165 20, reference by 135 20 165 20 0.4 -2.9 unbinned pixels; 11.2 2.8 nm along two shift axes; 11.1 2.3 nm on specimen axes Total_Shift = 5.834007 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.24 microns changed by -2.34 to target drift = 0.21 nm/sec Measured defocus = -2.11 microns changed by 0.01 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0878.tif 27 frames are being saved to U:\krios\20190717\PilA_0879.tif 27 frames are being saved to U:\krios\20190717\PilA_0880.tif 27 frames are being saved to U:\krios\20190717\PilA_0881.tif 27 frames are being saved to U:\krios\20190717\PilA_0882.tif 27 frames are being saved to U:\krios\20190717\PilA_0883.tif Running script 17 at item 197 (369.69, -224.47) Remaining time to fill dewars is 27000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 160 23 135 20, reference by 200 21 170 20 Disparity in stage position after aligning to target position: -0.07 -0.49 (2nd move error 0.06 -0.16) Finished aligning to item in 1 rounds Trimming A by 160 23 135 20, reference by 160 23 135 20 -21.6 -1.5 unbinned pixels; 22.7 79.8 nm along two shift axes; 10.3 -83.5 nm on specimen axes Total_Shift = 43.203566 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.36 microns changed by -3.56 to target drift = 0.15 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0884.tif 27 frames are being saved to U:\krios\20190717\PilA_0885.tif 27 frames are being saved to U:\krios\20190717\PilA_0886.tif 27 frames are being saved to U:\krios\20190717\PilA_0887.tif 27 frames are being saved to U:\krios\20190717\PilA_0888.tif 27 frames are being saved to U:\krios\20190717\PilA_0889.tif Running script 17 at item 198 (367.18, -222.73) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 158 22 145 20, reference by 200 21 170 20 Disparity in stage position after aligning to target position: -0.09 -0.59 (2nd move error 0.04 -0.26) Finished aligning to item in 1 rounds Trimming A by 153 23 145 20, reference by 158 22 145 20 -28.9 -0.1 unbinned pixels; 37.2 104.1 nm along two shift axes; 6.6 -112.3 nm on specimen axes Total_Shift = 57.738285 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.15 microns changed by -2.15 to target drift = 0.18 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0890.tif 27 frames are being saved to U:\krios\20190717\PilA_0891.tif 27 frames are being saved to U:\krios\20190717\PilA_0892.tif 27 frames are being saved to U:\krios\20190717\PilA_0893.tif 27 frames are being saved to U:\krios\20190717\PilA_0894.tif 27 frames are being saved to U:\krios\20190717\PilA_0895.tif Running script 17 at item 199 (363.05, -224.25) Remaining time to fill dewars is 26700 Dewars are NOT busy filling Trimming A by 220 21 20 240, reference by 210 23 20 225 Trimming A by 220 21 20 240, reference by 200 21 170 20 Trimming A by 220 21 20 240, reference by 20 205 20 220 Trimming A by 220 21 20 240, reference by 21 200 170 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.28 Trimming A by 150 22 20 120, reference by 210 23 20 225 Disparity in stage position after aligning to target position: 0.01 -0.08 (2nd move error 0.08 0.17) Finished aligning to item in 2 rounds Trimming A by 155 21 70 115, reference by 150 22 20 120 -1.9 0.6 unbinned pixels; 4.7 5.8 nm along two shift axes; -2.0 -7.4 nm on specimen axes Total_Shift = 3.93839 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.99 microns changed by -3.39 to target drift = 0.15 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0896.tif 27 frames are being saved to U:\krios\20190717\PilA_0897.tif 27 frames are being saved to U:\krios\20190717\PilA_0898.tif 27 frames are being saved to U:\krios\20190717\PilA_0899.tif 27 frames are being saved to U:\krios\20190717\PilA_0900.tif 27 frames are being saved to U:\krios\20190717\PilA_0901.tif Running script 17 at item 200 (365.62, -225.28) Remaining time to fill dewars is 26580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 20 85 20, reference by 200 21 170 20 Disparity in stage position after aligning to target position: -0.10 -0.63 (2nd move error 0.03 -0.29) Finished aligning to item in 1 rounds Trimming A by 123 21 85 20, reference by 130 20 85 20 -26.2 -0.7 unbinned pixels; 31.6 95.5 nm along two shift axes; 8.3 -101.9 nm on specimen axes Total_Shift = 52.4841 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.35 microns changed by -2.15 to target drift = 0.22 nm/sec Measured defocus = -2.52 microns changed by 0.02 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0902.tif 27 frames are being saved to U:\krios\20190717\PilA_0903.tif 27 frames are being saved to U:\krios\20190717\PilA_0904.tif 27 frames are being saved to U:\krios\20190717\PilA_0905.tif 27 frames are being saved to U:\krios\20190717\PilA_0906.tif 27 frames are being saved to U:\krios\20190717\PilA_0907.tif Running script 17 at item 201 (368.14, -226.98) Remaining time to fill dewars is 26400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 80 20 75 20, reference by 200 21 170 20 Disparity in stage position after aligning to target position: -0.03 -0.51 (2nd move error 0.10 -0.17) Finished aligning to item in 1 rounds Trimming A by 80 24 70 20, reference by 80 20 75 20 -20.8 -4.7 unbinned pixels; 9.7 82.0 nm along two shift axes; 22.8 -79.9 nm on specimen axes Total_Shift = 42.647069 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.96 microns changed by -3.56 to target drift = 0.21 nm/sec Measured defocus = -2.66 microns changed by 0.06 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0908.tif 27 frames are being saved to U:\krios\20190717\PilA_0909.tif 27 frames are being saved to U:\krios\20190717\PilA_0910.tif 27 frames are being saved to U:\krios\20190717\PilA_0911.tif 27 frames are being saved to U:\krios\20190717\PilA_0912.tif 27 frames are being saved to U:\krios\20190717\PilA_0913.tif Running script 17 at item 202 (370.66, -228.71) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Trimming A by 225 23 185 20, reference by 210 23 20 225 Trimming A by 225 23 185 20, reference by 200 21 170 20 Trimming A by 225 23 185 20, reference by 20 205 20 220 Trimming A by 225 23 185 20, reference by 21 200 170 20 Disparity in stage position after aligning to center of map frame: -0.10 -0.31 Trimming A by 70 50 95 20, reference by 200 21 170 20 Disparity in stage position after aligning to target position: 0.05 -0.40 (2nd move error 0.14 -0.11) Finished aligning to item in 2 rounds Trimming A by 65 55 100 20, reference by 70 50 95 20 -12.2 2.3 unbinned pixels; 24.6 40.6 nm along two shift axes; -6.5 -48.1 nm on specimen axes Total_Shift = 24.936817 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.19 microns changed by -2.29 to target drift = 0.09 nm/sec Measured defocus = -1.12 microns changed by 0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0914.tif 27 frames are being saved to U:\krios\20190717\PilA_0915.tif 27 frames are being saved to U:\krios\20190717\PilA_0916.tif 27 frames are being saved to U:\krios\20190717\PilA_0917.tif 27 frames are being saved to U:\krios\20190717\PilA_0918.tif 27 frames are being saved to U:\krios\20190717\PilA_0919.tif Running script 17 at item 203 (373.36, -230.34) Remaining time to fill dewars is 26100 Dewars are NOT busy filling Trimming A by 24 191 195 20, reference by 210 23 20 225 Trimming A by 24 191 195 20, reference by 200 21 170 20 Trimming A by 24 191 195 20, reference by 20 205 20 220 Trimming A by 24 191 195 20, reference by 21 200 170 20 Disparity in stage position after aligning to center of map frame: -0.07 -0.36 Trimming A by 24 97 190 20, reference by 21 200 170 20 Disparity in stage position after aligning to target position: -0.10 -0.30 (2nd move error -0.04 0.03) Finished aligning to item in 2 rounds Trimming A by 24 97 190 20, reference by 24 97 190 20 -1.0 9.8 unbinned pixels; 37.4 11.3 nm along two shift axes; -38.0 -5.9 nm on specimen axes Total_Shift = 19.741194 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.60 microns changed by -3.80 to target drift = 0.18 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0920.tif 27 frames are being saved to U:\krios\20190717\PilA_0921.tif 27 frames are being saved to U:\krios\20190717\PilA_0922.tif 27 frames are being saved to U:\krios\20190717\PilA_0923.tif 27 frames are being saved to U:\krios\20190717\PilA_0924.tif 27 frames are being saved to U:\krios\20190717\PilA_0925.tif Running script 17 at item 204 (371.68, -232.95) Remaining time to fill dewars is 25980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 135 155 20, reference by 21 200 170 20 Disparity in stage position after aligning to target position: -0.11 -0.43 (2nd move error -0.04 -0.09) Finished aligning to item in 1 rounds Trimming A by 21 127 165 20, reference by 24 135 155 20 -11.6 9.3 unbinned pixels; 49.2 28.0 nm along two shift axes; -33.6 -47.3 nm on specimen axes Total_Shift = 29.750988 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.07 microns changed by -2.37 to target drift = 0.05 nm/sec Measured defocus = -1.29 microns changed by -0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0926.tif 27 frames are being saved to U:\krios\20190717\PilA_0927.tif 27 frames are being saved to U:\krios\20190717\PilA_0928.tif 27 frames are being saved to U:\krios\20190717\PilA_0929.tif 27 frames are being saved to U:\krios\20190717\PilA_0930.tif 27 frames are being saved to U:\krios\20190717\PilA_0931.tif Running script 17 at item 205 (369.16, -231.21) Remaining time to fill dewars is 25860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 75 75 20, reference by 21 200 170 20 Disparity in stage position after aligning to target position: -0.13 -0.58 (2nd move error -0.07 -0.24) Finished aligning to item in 1 rounds Trimming A by 22 65 85 20, reference by 24 75 75 20 -28.8 13.8 unbinned pixels; 88.3 82.8 nm along two shift axes; -47.6 -114.8 nm on specimen axes Total_Shift = 63.816195 Trimming A by 24 80 75 20, reference by 24 75 75 20 -1.4 7.1 unbinned pixels; 27.8 5.7 nm along two shift axes; -27.2 -6.8 nm on specimen axes Total_Shift = 14.384118 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.25 microns changed by -3.65 to target drift = 0.05 nm/sec Measured defocus = -1.39 microns changed by -0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0932.tif 27 frames are being saved to U:\krios\20190717\PilA_0933.tif 27 frames are being saved to U:\krios\20190717\PilA_0934.tif 27 frames are being saved to U:\krios\20190717\PilA_0935.tif 27 frames are being saved to U:\krios\20190717\PilA_0936.tif 27 frames are being saved to U:\krios\20190717\PilA_0937.tif Running script 17 at item 206 (366.60, -229.56) Remaining time to fill dewars is 25680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 200 21 170 20 Disparity in stage position after aligning to target position: 0.04 -0.61 (2nd move error 0.13 -0.32) Finished aligning to item in 1 rounds -24.0 -3.5 unbinned pixels; 18.3 91.6 nm along two shift axes; 18.8 -92.5 nm on specimen axes Total_Shift = 48.434137 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.78 microns changed by -2.28 to target drift = 0.19 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0938.tif 27 frames are being saved to U:\krios\20190717\PilA_0939.tif 27 frames are being saved to U:\krios\20190717\PilA_0940.tif 27 frames are being saved to U:\krios\20190717\PilA_0941.tif 27 frames are being saved to U:\krios\20190717\PilA_0942.tif 27 frames are being saved to U:\krios\20190717\PilA_0943.tif Running script 17 at item 207 (363.79, -228.46) Remaining time to fill dewars is 25560 Dewars are NOT busy filling Trimming A by 209 24 20 250, reference by 210 23 20 225 Trimming A by 209 24 20 250, reference by 200 21 170 20 Trimming A by 209 24 20 250, reference by 20 205 20 220 Trimming A by 209 24 20 250, reference by 21 200 170 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.29 Trimming A by 50 23 20 75, reference by 210 23 20 225 Disparity in stage position after aligning to target position: 0.21 -0.10 (2nd move error 0.28 0.17) Finished aligning to item in 2 rounds Trimming A by 46 24 20 75, reference by 50 23 20 75 1.4 -9.2 unbinned pixels; 35.8 8.8 nm along two shift axes; 35.7 7.5 nm on specimen axes Total_Shift = 18.705152 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.85 microns changed by -3.45 to target drift = 0.17 nm/sec Measured defocus = -1.60 microns changed by -0.00 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0944.tif 27 frames are being saved to U:\krios\20190717\PilA_0945.tif 27 frames are being saved to U:\krios\20190717\PilA_0946.tif 27 frames are being saved to U:\krios\20190717\PilA_0947.tif 27 frames are being saved to U:\krios\20190717\PilA_0948.tif 27 frames are being saved to U:\krios\20190717\PilA_0949.tif Running script 17 at item 208 (361.47, -226.78) Remaining time to fill dewars is 25380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 120 20 20 140, reference by 210 23 20 225 Disparity in stage position after aligning to target position: 0.08 -0.19 (2nd move error 0.16 0.08) Finished aligning to item in 1 rounds Trimming A by 106 21 20 150, reference by 120 20 20 140 0.6 -5.5 unbinned pixels; 21.0 6.3 nm along two shift axes; 21.3 3.3 nm on specimen axes Total_Shift = 11.086271 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.29 microns changed by -1.99 to target drift = 0.15 nm/sec Measured defocus = -1.66 microns changed by -0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0950.tif 27 frames are being saved to U:\krios\20190717\PilA_0951.tif 27 frames are being saved to U:\krios\20190717\PilA_0952.tif 27 frames are being saved to U:\krios\20190717\PilA_0953.tif 27 frames are being saved to U:\krios\20190717\PilA_0954.tif 27 frames are being saved to U:\krios\20190717\PilA_0955.tif Running script 17 at item 209 (362.52, -230.99) Remaining time to fill dewars is 25200 Dewars are NOT busy filling Trimming A by 20 210 20 230, reference by 210 23 20 225 Trimming A by 20 210 20 230, reference by 200 21 170 20 Trimming A by 20 210 20 230, reference by 20 205 20 220 Trimming A by 20 210 20 230, reference by 21 200 170 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.32 Trimming A by 21 65 20 95, reference by 20 205 20 220 Disparity in stage position after aligning to target position: 0.00 -0.16 (2nd move error 0.03 0.13) Finished aligning to item in 2 rounds Trimming A by 21 60 20 95, reference by 21 65 20 95 -0.4 -1.1 unbinned pixels; 3.4 3.2 nm along two shift axes; 4.3 -1.4 nm on specimen axes Total_Shift = 2.320127 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.57 microns changed by -3.37 to target drift = 0.17 nm/sec Measured defocus = -1.80 microns changed by -0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0956.tif 27 frames are being saved to U:\krios\20190717\PilA_0957.tif 27 frames are being saved to U:\krios\20190717\PilA_0958.tif 27 frames are being saved to U:\krios\20190717\PilA_0959.tif 27 frames are being saved to U:\krios\20190717\PilA_0960.tif 27 frames are being saved to U:\krios\20190717\PilA_0961.tif Running script 17 at item 210 (364.66, -232.57) Remaining time to fill dewars is 25080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 20 80, reference by 20 205 20 220 Disparity in stage position after aligning to target position: 0.03 -0.09 (2nd move error 0.07 0.20) Finished aligning to item in 1 rounds Trimming A by 20 75 20 75, reference by 20 80 20 80 -0.7 0.5 unbinned pixels; 2.7 1.8 nm along two shift axes; -1.7 -2.8 nm on specimen axes Total_Shift = 1.705109 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.07 microns changed by -1.97 to target drift = 0.15 nm/sec Measured defocus = -1.83 microns changed by -0.07 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0962.tif 27 frames are being saved to U:\krios\20190717\PilA_0963.tif 27 frames are being saved to U:\krios\20190717\PilA_0964.tif 27 frames are being saved to U:\krios\20190717\PilA_0965.tif 27 frames are being saved to U:\krios\20190717\PilA_0966.tif 27 frames are being saved to U:\krios\20190717\PilA_0967.tif Running script 17 at item 211 (367.54, -233.79) Remaining time to fill dewars is 25020 Dewars are NOT busy filling Trimming A by 22 200 190 20, reference by 210 23 20 225 Trimming A by 22 200 190 20, reference by 200 21 170 20 Trimming A by 22 200 190 20, reference by 20 205 20 220 Trimming A by 22 200 190 20, reference by 21 200 170 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.30 Trimming A by 23 100 65 25, reference by 21 200 170 20 Disparity in stage position after aligning to target position: -0.13 -0.63 (2nd move error -0.10 -0.35) Finished aligning to item in 2 rounds Trimming A by 24 105 65 30, reference by 23 100 65 25 -23.6 17.9 unbinned pixels; 96.5 57.9 nm along two shift axes; -64.6 -95.4 nm on specimen axes Total_Shift = 59.152682 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.55 microns changed by -3.55 to target drift = 0.05 nm/sec Measured defocus = -2.03 microns changed by 0.03 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0968.tif 27 frames are being saved to U:\krios\20190717\PilA_0969.tif 27 frames are being saved to U:\krios\20190717\PilA_0970.tif 27 frames are being saved to U:\krios\20190717\PilA_0971.tif 27 frames are being saved to U:\krios\20190717\PilA_0972.tif 27 frames are being saved to U:\krios\20190717\PilA_0973.tif Running script 17 at item 212 (370.05, -235.54) Remaining time to fill dewars is 24720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 170 130 20, reference by 21 200 170 20 Disparity in stage position after aligning to target position: 0.00 -0.48 (2nd move error 0.04 -0.20) Finished aligning to item in 1 rounds Trimming A by 24 165 135 20, reference by 22 170 130 20 -25.4 -1.8 unbinned pixels; 26.5 94.0 nm along two shift axes; 12.3 -98.3 nm on specimen axes Total_Shift = 50.877587 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.15 microns changed by -2.25 to target drift = 0.17 nm/sec Measured defocus = -2.07 microns changed by -0.03 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0974.tif 27 frames are being saved to U:\krios\20190717\PilA_0975.tif 27 frames are being saved to U:\krios\20190717\PilA_0976.tif 27 frames are being saved to U:\krios\20190717\PilA_0977.tif 27 frames are being saved to U:\krios\20190717\PilA_0978.tif 27 frames are being saved to U:\krios\20190717\PilA_0979.tif Running script 17 at item 213 (396.81, -317.96) Remaining time to fill dewars is 24600 Dewars are NOT busy filling Trimming A by 23 170 185 20, reference by 217 23 20 225 Trimming A by 23 170 185 20, reference by 249 22 200 20 Trimming A by 23 170 185 20, reference by 24 145 20 165 Trimming A by 23 170 185 20, reference by 22 160 170 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.27 Trimming A by 23 110 180 20, reference by 22 160 170 20 Disparity in stage position after aligning to target position: -0.11 -0.30 (2nd move error -0.02 -0.06) Finished aligning to item in 2 rounds Trimming A by 20 111 180 20, reference by 23 110 180 20 -7.1 5.0 unbinned pixels; 27.5 18.0 nm along two shift axes; -17.8 -28.6 nm on specimen axes Total_Shift = 17.292051 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.70 microns changed by -3.90 to target drift = 0.12 nm/sec Measured defocus = -2.25 microns changed by 0.05 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0980.tif 27 frames are being saved to U:\krios\20190717\PilA_0981.tif 27 frames are being saved to U:\krios\20190717\PilA_0982.tif 27 frames are being saved to U:\krios\20190717\PilA_0983.tif 27 frames are being saved to U:\krios\20190717\PilA_0984.tif 27 frames are being saved to U:\krios\20190717\PilA_0985.tif Running script 17 at item 214 (394.26, -316.22) Remaining time to fill dewars is 24480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 42 105 20, reference by 22 160 170 20 Disparity in stage position after aligning to target position: -0.11 -0.49 (2nd move error -0.02 -0.25) Finished aligning to item in 1 rounds Trimming A by 22 38 110 20, reference by 22 42 105 20 -27.0 5.2 unbinned pixels; 54.4 89.5 nm along two shift axes; -14.6 -106.3 nm on specimen axes Total_Shift = 55.08119 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.12 microns changed by -2.42 to target drift = 0.08 nm/sec Measured defocus = -2.32 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0986.tif 27 frames are being saved to U:\krios\20190717\PilA_0987.tif 27 frames are being saved to U:\krios\20190717\PilA_0988.tif 27 frames are being saved to U:\krios\20190717\PilA_0989.tif 27 frames are being saved to U:\krios\20190717\PilA_0990.tif 27 frames are being saved to U:\krios\20190717\PilA_0991.tif Running script 17 at item 215 (393.21, -312.03) Remaining time to fill dewars is 24360 Dewars are NOT busy filling Trimming A by 276 23 210 20, reference by 217 23 20 225 Trimming A by 276 23 210 20, reference by 249 22 200 20 Trimming A by 276 23 210 20, reference by 24 145 20 165 Trimming A by 276 23 210 20, reference by 22 160 170 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.30 Trimming A by 110 20 110 20, reference by 249 22 200 20 Disparity in stage position after aligning to target position: -0.04 -0.51 (2nd move error 0.04 -0.24) Finished aligning to item in 2 rounds Trimming A by 115 23 105 20, reference by 110 20 110 20 -25.4 -3.1 unbinned pixels; 21.7 96.2 nm along two shift axes; 17.5 -98.2 nm on specimen axes Total_Shift = 51.220472 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.33 microns changed by -3.73 to target drift = 0.16 nm/sec Measured defocus = -2.43 microns changed by 0.03 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0992.tif 27 frames are being saved to U:\krios\20190717\PilA_0993.tif 27 frames are being saved to U:\krios\20190717\PilA_0994.tif 27 frames are being saved to U:\krios\20190717\PilA_0995.tif 27 frames are being saved to U:\krios\20190717\PilA_0996.tif 27 frames are being saved to U:\krios\20190717\PilA_0997.tif Running script 17 at item 216 (390.67, -310.32) Remaining time to fill dewars is 24180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 24 85 20, reference by 249 22 200 20 Disparity in stage position after aligning to target position: -0.03 -0.59 (2nd move error 0.05 -0.32) Finished aligning to item in 1 rounds Trimming A by 130 20 80 20, reference by 135 24 85 20 -33.4 -2.1 unbinned pixels; 35.8 123.5 nm along two shift axes; 15.3 -129.5 nm on specimen axes Total_Shift = 66.963757 Trimming A by 125 21 80 20, reference by 135 24 85 20 -2.5 -2.9 unbinned pixels; 7.3 13.5 nm along two shift axes; 11.8 -9.3 nm on specimen axes Total_Shift = 7.696219 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.17 microns changed by -2.33 to target drift = 0.19 nm/sec Measured defocus = -2.53 microns changed by 0.03 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_0998.tif 27 frames are being saved to U:\krios\20190717\PilA_0999.tif 27 frames are being saved to U:\krios\20190717\PilA_1000.tif 27 frames are being saved to U:\krios\20190717\PilA_1001.tif 27 frames are being saved to U:\krios\20190717\PilA_1002.tif 27 frames are being saved to U:\krios\20190717\PilA_1003.tif Running script 17 at item 217 (386.43, -311.82) Remaining time to fill dewars is 23940 Dewars are NOT busy filling Trimming A by 249 23 20 230, reference by 217 23 20 225 Trimming A by 249 23 20 230, reference by 249 22 200 20 Trimming A by 249 23 20 230, reference by 24 145 20 165 Trimming A by 249 23 20 230, reference by 22 160 170 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.28 Trimming A by 104 24 20 140, reference by 217 23 20 225 Disparity in stage position after aligning to target position: 0.13 -0.15 (2nd move error 0.18 0.11) Finished aligning to item in 2 rounds Trimming A by 110 22 20 135, reference by 104 24 20 140 0.6 -0.7 unbinned pixels; 3.3 1.2 nm along two shift axes; 2.5 2.6 nm on specimen axes Total_Shift = 1.849016 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.88 microns changed by -3.48 to target drift = 0.12 nm/sec Measured defocus = -2.62 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1004.tif 27 frames are being saved to U:\krios\20190717\PilA_1005.tif 27 frames are being saved to U:\krios\20190717\PilA_1006.tif 27 frames are being saved to U:\krios\20190717\PilA_1007.tif 27 frames are being saved to U:\krios\20190717\PilA_1008.tif 27 frames are being saved to U:\krios\20190717\PilA_1009.tif Running script 17 at item 218 (389.10, -313.36) Remaining time to fill dewars is 23760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 20 20 55, reference by 217 23 20 225 Disparity in stage position after aligning to target position: 0.21 -0.02 (2nd move error 0.25 0.24) Finished aligning to item in 1 rounds Trimming A by 60 20 20 50, reference by 65 20 20 55 -1.2 0.9 unbinned pixels; 4.9 3.1 nm along two shift axes; -3.2 -5.0 nm on specimen axes Total_Shift = 3.059959 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.22 microns changed by -2.32 to target drift = 0.16 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1010.tif 27 frames are being saved to U:\krios\20190717\PilA_1011.tif 27 frames are being saved to U:\krios\20190717\PilA_1012.tif 27 frames are being saved to U:\krios\20190717\PilA_1013.tif 27 frames are being saved to U:\krios\20190717\PilA_1014.tif 27 frames are being saved to U:\krios\20190717\PilA_1015.tif Running script 17 at item 219 (391.79, -314.47) Remaining time to fill dewars is 23640 Dewars are NOT busy filling Trimming A by 24 165 185 20, reference by 217 23 20 225 Trimming A by 24 165 185 20, reference by 249 22 200 20 Trimming A by 24 165 185 20, reference by 24 145 20 165 Trimming A by 24 165 185 20, reference by 22 160 170 20 Disparity in stage position after aligning to center of map frame: -0.02 -0.27 Trimming A by 50 20 45 20, reference by 22 160 170 20 Disparity in stage position after aligning to target position: -0.15 -0.65 (2nd move error -0.13 -0.40) Finished aligning to item in 2 rounds Trimming A by 40 20 45 20, reference by 50 20 45 20 -35.8 24.5 unbinned pixels; 136.8 91.9 nm along two shift axes; -87.8 -144.4 nm on specimen axes Total_Shift = 86.736611 Trimming A by 45 21 40 20, reference by 50 20 45 20 -3.0 14.8 unbinned pixels; 58.4 11.5 nm along two shift axes; -57.0 -14.8 nm on specimen axes Total_Shift = 30.231606 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.60 microns changed by -3.80 to target drift = 0.03 nm/sec Measured defocus = -1.25 microns changed by 0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1016.tif 27 frames are being saved to U:\krios\20190717\PilA_1017.tif 27 frames are being saved to U:\krios\20190717\PilA_1018.tif 27 frames are being saved to U:\krios\20190717\PilA_1019.tif 27 frames are being saved to U:\krios\20190717\PilA_1020.tif 27 frames are being saved to U:\krios\20190717\PilA_1021.tif Running script 17 at item 220 (392.63, -318.82) Remaining time to fill dewars is 23460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 80 75 20, reference by 22 160 170 20 Disparity in stage position after aligning to target position: -0.08 -0.61 (2nd move error -0.06 -0.36) Finished aligning to item in 1 rounds Trimming A by 24 85 75 20, reference by 24 80 75 20 -32.3 11.8 unbinned pixels; 85.3 98.4 nm along two shift axes; -38.9 -127.9 nm on specimen axes Total_Shift = 68.652625 Trimming A by 24 85 80 20, reference by 24 80 75 20 -3.8 5.1 unbinned pixels; 23.8 5.8 nm along two shift axes; -19.2 -15.7 nm on specimen axes Total_Shift = 12.73934 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.21 microns changed by -2.51 to target drift = 0.02 nm/sec Measured defocus = -1.36 microns changed by 0.06 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1022.tif 27 frames are being saved to U:\krios\20190717\PilA_1023.tif 27 frames are being saved to U:\krios\20190717\PilA_1024.tif 27 frames are being saved to U:\krios\20190717\PilA_1025.tif 27 frames are being saved to U:\krios\20190717\PilA_1026.tif 27 frames are being saved to U:\krios\20190717\PilA_1027.tif Running script 17 at item 221 (395.12, -320.56) Remaining time to fill dewars is 23220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 150 145 20, reference by 22 160 170 20 Disparity in stage position after aligning to target position: -0.05 -0.42 (2nd move error -0.03 -0.17) Finished aligning to item in 1 rounds Trimming A by 20 137 160 20, reference by 21 150 145 20 -20.3 6.7 unbinned pixels; 51.3 63.1 nm along two shift axes; -21.9 -80.5 nm on specimen axes Total_Shift = 42.828954 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.49 microns changed by -3.89 to target drift = 0.02 nm/sec Measured defocus = -1.43 microns changed by 0.03 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1028.tif 27 frames are being saved to U:\krios\20190717\PilA_1029.tif 27 frames are being saved to U:\krios\20190717\PilA_1030.tif 27 frames are being saved to U:\krios\20190717\PilA_1031.tif 27 frames are being saved to U:\krios\20190717\PilA_1032.tif 27 frames are being saved to U:\krios\20190717\PilA_1033.tif Running script 17 at item 222 (390.10, -317.03) Remaining time to fill dewars is 23040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 22 160 170 20 Disparity in stage position after aligning to target position: -0.07 -0.76 (2nd move error -0.06 -0.51) Finished aligning to item in 1 rounds Trimming A by 20 20 20 25, reference by 20 20 20 20 -33.8 9.7 unbinned pixels; 79.6 107.0 nm along two shift axes; -30.5 -133.4 nm on specimen axes Total_Shift = 70.243133 Trimming A by 20 31 20 20, reference by 20 20 20 20 1.9 4.2 unbinned pixels; 13.1 13.1 nm along two shift axes; -16.8 6.4 nm on specimen axes Total_Shift = 9.222566 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.86 microns changed by -2.36 to target drift = 0.06 nm/sec Measured defocus = -1.47 microns changed by -0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1034.tif 27 frames are being saved to U:\krios\20190717\PilA_1035.tif 27 frames are being saved to U:\krios\20190717\PilA_1036.tif 27 frames are being saved to U:\krios\20190717\PilA_1037.tif 27 frames are being saved to U:\krios\20190717\PilA_1038.tif 27 frames are being saved to U:\krios\20190717\PilA_1039.tif Running script 17 at item 223 (387.62, -316.07) Remaining time to fill dewars is 22980 Dewars are NOT busy filling Trimming A by 20 150 20 170, reference by 217 23 20 225 Trimming A by 20 150 20 170, reference by 249 22 200 20 Trimming A by 20 150 20 170, reference by 24 145 20 165 Trimming A by 20 150 20 170, reference by 22 160 170 20 Disparity in stage position after aligning to center of map frame: -0.07 -0.32 Trimming A by 40 20 20 50, reference by 24 145 20 165 Disparity in stage position after aligning to target position: -0.05 -0.14 (2nd move error 0.02 0.15) Finished aligning to item in 2 rounds Trimming A by 40 21 20 55, reference by 40 20 20 50 -1.0 0.8 unbinned pixels; 4.1 2.4 nm along two shift axes; -2.8 -4.0 nm on specimen axes Total_Shift = 2.487957 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.96 microns changed by -3.56 to target drift = 0.15 nm/sec Measured defocus = -1.58 microns changed by -0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1040.tif 27 frames are being saved to U:\krios\20190717\PilA_1041.tif 27 frames are being saved to U:\krios\20190717\PilA_1042.tif 27 frames are being saved to U:\krios\20190717\PilA_1043.tif 27 frames are being saved to U:\krios\20190717\PilA_1044.tif 27 frames are being saved to U:\krios\20190717\PilA_1045.tif Running script 17 at item 224 (385.12, -314.20) Remaining time to fill dewars is 22680 Dewars are NOT busy filling Trimming A by 246 21 20 235, reference by 217 23 20 225 Trimming A by 246 21 20 235, reference by 249 22 200 20 Trimming A by 246 21 20 235, reference by 24 145 20 165 Trimming A by 246 21 20 235, reference by 22 160 170 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.30 Trimming A by 105 20 20 125, reference by 217 23 20 225 Disparity in stage position after aligning to target position: 0.19 -0.24 (2nd move error 0.24 0.02) Finished aligning to item in 2 rounds Trimming A by 105 24 20 120, reference by 105 20 20 125 -1.1 -1.5 unbinned pixels; 3.9 6.2 nm along two shift axes; 5.9 -4.0 nm on specimen axes Total_Shift = 3.656888 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.47 microns changed by -2.17 to target drift = 0.12 nm/sec Measured defocus = -1.70 microns changed by 0.00 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1046.tif 27 frames are being saved to U:\krios\20190717\PilA_1047.tif 27 frames are being saved to U:\krios\20190717\PilA_1048.tif 27 frames are being saved to U:\krios\20190717\PilA_1049.tif 27 frames are being saved to U:\krios\20190717\PilA_1050.tif 27 frames are being saved to U:\krios\20190717\PilA_1051.tif Running script 17 at item 225 (383.52, -316.86) Remaining time to fill dewars is 22440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 65 20 140, reference by 24 145 20 165 Disparity in stage position after aligning to target position: -0.11 -0.37 (2nd move error -0.05 -0.08) Finished aligning to item in 1 rounds Trimming A by 56 56 20 150, reference by 65 65 20 140 -9.5 9.8 unbinned pixels; 48.4 19.4 nm along two shift axes; -36.1 -39.0 nm on specimen axes Total_Shift = 27.261146 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.62 microns changed by -3.42 to target drift = 0.08 nm/sec Measured defocus = -1.80 microns changed by 0.00 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1052.tif 27 frames are being saved to U:\krios\20190717\PilA_1053.tif 27 frames are being saved to U:\krios\20190717\PilA_1054.tif 27 frames are being saved to U:\krios\20190717\PilA_1055.tif 27 frames are being saved to U:\krios\20190717\PilA_1056.tif 27 frames are being saved to U:\krios\20190717\PilA_1057.tif Running script 17 at item 226 (386.02, -318.57) Remaining time to fill dewars is 22320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 85 20 110, reference by 24 145 20 165 Disparity in stage position after aligning to target position: -0.03 -0.25 (2nd move error 0.04 0.04) Finished aligning to item in 1 rounds Trimming A by 21 80 20 110, reference by 21 85 20 110 -2.5 -1.5 unbinned pixels; 2.1 11.2 nm along two shift axes; 6.2 -9.4 nm on specimen axes Total_Shift = 5.781867 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.24 microns changed by -2.14 to target drift = 0.14 nm/sec Measured defocus = -1.86 microns changed by -0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1058.tif 27 frames are being saved to U:\krios\20190717\PilA_1059.tif 27 frames are being saved to U:\krios\20190717\PilA_1060.tif 27 frames are being saved to U:\krios\20190717\PilA_1061.tif 27 frames are being saved to U:\krios\20190717\PilA_1062.tif 27 frames are being saved to U:\krios\20190717\PilA_1063.tif Running script 17 at item 227 (388.51, -320.33) Remaining time to fill dewars is 22140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 105 55 90, reference by 24 145 20 165 Disparity in stage position after aligning to target position: 0.03 -0.08 (2nd move error 0.10 0.21) Finished aligning to item in 1 rounds Trimming A by 24 100 20 85, reference by 21 105 55 90 0.6 -2.4 unbinned pixels; 9.5 1.4 nm along two shift axes; 9.1 2.9 nm on specimen axes Total_Shift = 4.89967 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.53 microns changed by -3.52 to target drift = 0.14 nm/sec Measured defocus = -2.02 microns changed by 0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1064.tif 27 frames are being saved to U:\krios\20190717\PilA_1065.tif 27 frames are being saved to U:\krios\20190717\PilA_1066.tif 27 frames are being saved to U:\krios\20190717\PilA_1067.tif 27 frames are being saved to U:\krios\20190717\PilA_1068.tif 27 frames are being saved to U:\krios\20190717\PilA_1069.tif Running script 17 at item 228 (391.02, -321.43) Remaining time to fill dewars is 21960 Dewars are NOT busy filling Trimming A by 21 160 190 20, reference by 217 23 20 225 Trimming A by 21 160 190 20, reference by 249 22 200 20 Trimming A by 21 160 190 20, reference by 24 145 20 165 Trimming A by 21 160 190 20, reference by 22 160 170 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.26 Trimming A by 20 110 60 65, reference by 22 160 170 20 Disparity in stage position after aligning to target position: -0.05 -0.66 (2nd move error -0.07 -0.43) Finished aligning to item in 2 rounds Trimming A by 20 120 60 60, reference by 20 110 60 65 -32.2 14.1 unbinned pixels; 93.9 94.7 nm along two shift axes; -48.1 -128.3 nm on specimen axes Total_Shift = 70.328471 Trimming A by 20 120 60 70, reference by 20 110 60 65 0.7 7.2 unbinned pixels; 25.8 13.2 nm along two shift axes; -28.3 1.0 nm on specimen axes Total_Shift = 14.540006 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.21 microns changed by -2.31 to target drift = 0.06 nm/sec Measured defocus = -2.08 microns changed by -0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1070.tif 27 frames are being saved to U:\krios\20190717\PilA_1071.tif 27 frames are being saved to U:\krios\20190717\PilA_1072.tif 27 frames are being saved to U:\krios\20190717\PilA_1073.tif 27 frames are being saved to U:\krios\20190717\PilA_1074.tif 27 frames are being saved to U:\krios\20190717\PilA_1075.tif Running script 17 at item 248-1 (450.11, -344.80) Remaining time to fill dewars is 21780 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 22 166 190 20, reference by 225 22 20 220 Trimming A by 22 166 190 20, reference by 240 21 180 20 Trimming A by 22 166 190 20, reference by 20 155 20 175 Trimming A by 22 166 190 20, reference by 24 170 170 20 Disparity in stage position after aligning to center of map frame: -0.24 -0.25 Trimming A by 75 70 130 20, reference by 24 170 170 20 Disparity in stage position after aligning to target position: -0.32 -0.28 (2nd move error -0.09 -0.05) Finished aligning to item in 2 rounds Trimming A by 75 70 130 20, reference by 75 70 130 20 -9.1 22.6 unbinned pixels; 94.9 1.2 nm along two shift axes; -85.9 -40.2 nm on specimen axes Total_Shift = 48.687993 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.96 microns changed by -4.16 to target drift = 0.09 nm/sec Measured defocus = -2.28 microns changed by 0.08 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1076.tif 27 frames are being saved to U:\krios\20190717\PilA_1077.tif 27 frames are being saved to U:\krios\20190717\PilA_1078.tif 27 frames are being saved to U:\krios\20190717\PilA_1079.tif 27 frames are being saved to U:\krios\20190717\PilA_1080.tif 27 frames are being saved to U:\krios\20190717\PilA_1081.tif Running script 17 at item 249-1 (447.37, -343.18) Remaining time to fill dewars is 21540 Dewars are NOT busy filling Trimming A by 254 24 195 20, reference by 225 22 20 220 Trimming A by 254 24 195 20, reference by 240 21 180 20 Trimming A by 254 24 195 20, reference by 20 155 20 175 Trimming A by 254 24 195 20, reference by 24 170 170 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.25 Trimming A by 95 23 95 20, reference by 240 21 180 20 Disparity in stage position after aligning to target position: -0.07 -0.37 (2nd move error 0.12 -0.14) Finished aligning to item in 2 rounds Trimming A by 95 23 95 20, reference by 95 23 95 20 -13.0 -7.7 unbinned pixels; 11.4 58.5 nm along two shift axes; 32.8 -49.0 nm on specimen axes Total_Shift = 30.286301 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.37 microns changed by -2.67 to target drift = 0.18 nm/sec Measured defocus = -2.27 microns changed by -0.03 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1082.tif 27 frames are being saved to U:\krios\20190717\PilA_1083.tif 27 frames are being saved to U:\krios\20190717\PilA_1084.tif 27 frames are being saved to U:\krios\20190717\PilA_1085.tif 27 frames are being saved to U:\krios\20190717\PilA_1086.tif 27 frames are being saved to U:\krios\20190717\PilA_1087.tif Running script 17 at item 250-1 (444.82, -341.43) Remaining time to fill dewars is 21240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 110 22 80 20, reference by 240 21 180 20 Disparity in stage position after aligning to target position: -0.03 -0.49 (2nd move error 0.17 -0.26) Finished aligning to item in 1 rounds Trimming A by 110 22 75 20, reference by 110 22 80 20 -23.5 -2.8 unbinned pixels; 20.5 88.9 nm along two shift axes; 15.8 -90.9 nm on specimen axes Total_Shift = 47.369677 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.24 microns changed by -2.64 to target drift = 0.19 nm/sec Measured defocus = -2.41 microns changed by 0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1088.tif 27 frames are being saved to U:\krios\20190717\PilA_1089.tif 27 frames are being saved to U:\krios\20190717\PilA_1090.tif 27 frames are being saved to U:\krios\20190717\PilA_1091.tif 27 frames are being saved to U:\krios\20190717\PilA_1092.tif 27 frames are being saved to U:\krios\20190717\PilA_1093.tif Running script 17 at item 251-1 (443.25, -344.04) Remaining time to fill dewars is 21120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 40 20 20 20, reference by 240 21 180 20 Disparity in stage position after aligning to target position: -0.05 -0.54 (2nd move error 0.15 -0.32) Finished aligning to item in 1 rounds Trimming A by 30 20 20 20, reference by 40 20 20 20 -26.4 -4.9 unbinned pixels; 16.5 102.4 nm along two shift axes; 24.6 -101.7 nm on specimen axes Total_Shift = 53.689819 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.11 microns changed by -2.61 to target drift = 0.22 nm/sec Measured defocus = -2.53 microns changed by 0.03 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1094.tif 27 frames are being saved to U:\krios\20190717\PilA_1095.tif 27 frames are being saved to U:\krios\20190717\PilA_1096.tif 27 frames are being saved to U:\krios\20190717\PilA_1097.tif 27 frames are being saved to U:\krios\20190717\PilA_1098.tif 27 frames are being saved to U:\krios\20190717\PilA_1099.tif Running script 17 at item 252-1 (445.95, -345.65) Remaining time to fill dewars is 20880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 25 21 35 20, reference by 24 170 170 20 Disparity in stage position after aligning to target position: -0.25 -0.47 (2nd move error -0.02 -0.25) Finished aligning to item in 1 rounds Trimming A by 20 20 35 20, reference by 25 21 35 20 -25.0 3.0 unbinned pixels; 43.8 85.6 nm along two shift axes; -6.5 -98.0 nm on specimen axes Total_Shift = 50.439245 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.07 microns changed by -2.67 to target drift = 0.11 nm/sec Measured defocus = -2.67 microns changed by 0.07 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1100.tif 27 frames are being saved to U:\krios\20190717\PilA_1101.tif 27 frames are being saved to U:\krios\20190717\PilA_1102.tif 27 frames are being saved to U:\krios\20190717\PilA_1103.tif 27 frames are being saved to U:\krios\20190717\PilA_1104.tif 27 frames are being saved to U:\krios\20190717\PilA_1105.tif Running script 17 at item 253-1 (448.51, -347.39) Remaining time to fill dewars is 20760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 65 115 20, reference by 24 170 170 20 Disparity in stage position after aligning to target position: -0.15 -0.39 (2nd move error 0.09 -0.17) Finished aligning to item in 1 rounds Trimming A by 24 70 115 20, reference by 21 65 115 20 -18.8 -2.3 unbinned pixels; 16.1 71.1 nm along two shift axes; 12.8 -72.6 nm on specimen axes Total_Shift = 37.842209 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.74 microns changed by -2.84 to target drift = 0.12 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1106.tif 27 frames are being saved to U:\krios\20190717\PilA_1107.tif 27 frames are being saved to U:\krios\20190717\PilA_1108.tif 27 frames are being saved to U:\krios\20190717\PilA_1109.tif 27 frames are being saved to U:\krios\20190717\PilA_1110.tif 27 frames are being saved to U:\krios\20190717\PilA_1111.tif Running script 17 at item 254-1 (446.78, -349.99) Remaining time to fill dewars is 20580 Dewars are NOT busy filling Trimming A by 23 170 185 20, reference by 225 22 20 220 Trimming A by 23 170 185 20, reference by 240 21 180 20 Trimming A by 23 170 185 20, reference by 20 155 20 175 Trimming A by 23 170 185 20, reference by 24 170 170 20 Disparity in stage position after aligning to center of map frame: -0.18 -0.25 Trimming A by 22 105 95 20, reference by 24 170 170 20 Disparity in stage position after aligning to target position: -0.22 -0.47 (2nd move error -0.05 -0.24) Finished aligning to item in 2 rounds Trimming A by 24 87 110 20, reference by 22 105 95 20 -27.4 8.8 unbinned pixels; 68.2 85.4 nm along two shift axes; -28.5 -108.5 nm on specimen axes Total_Shift = 57.584388 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 3.00 microns changed by -4.20 to target drift = 0.03 nm/sec Measured defocus = -1.26 microns changed by 0.06 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1112.tif 27 frames are being saved to U:\krios\20190717\PilA_1113.tif 27 frames are being saved to U:\krios\20190717\PilA_1114.tif 27 frames are being saved to U:\krios\20190717\PilA_1115.tif 27 frames are being saved to U:\krios\20190717\PilA_1116.tif 27 frames are being saved to U:\krios\20190717\PilA_1117.tif Running script 17 at item 255-1 (444.32, -348.25) Remaining time to fill dewars is 20400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 36 30 20, reference by 24 170 170 20 Disparity in stage position after aligning to target position: -0.20 -0.57 (2nd move error -0.03 -0.34) Finished aligning to item in 1 rounds Trimming A by 20 40 30 20, reference by 20 36 30 20 -32.8 7.0 unbinned pixels; 68.6 107.7 nm along two shift axes; -20.3 -129.2 nm on specimen axes Total_Shift = 67.160705 Trimming A by 20 40 30 20, reference by 20 36 30 20 -2.6 4.2 unbinned pixels; 18.9 3.0 nm along two shift axes; -15.9 -10.9 nm on specimen axes Total_Shift = 9.88848 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.43 microns changed by -2.73 to target drift = 0.06 nm/sec Measured defocus = -1.30 microns changed by -0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1118.tif 27 frames are being saved to U:\krios\20190717\PilA_1119.tif 27 frames are being saved to U:\krios\20190717\PilA_1120.tif 27 frames are being saved to U:\krios\20190717\PilA_1121.tif 27 frames are being saved to U:\krios\20190717\PilA_1122.tif 27 frames are being saved to U:\krios\20190717\PilA_1123.tif Running script 17 at item 256-1 (474.95, -430.09) Remaining time to fill dewars is 20100 Dewars are NOT busy filling Trimming A by 23 76 95 20, reference by 270 20 20 270 Trimming A by 23 76 95 20, reference by 210 21 135 20 Trimming A by 23 76 95 20, reference by 20 136 20 205 Trimming A by 23 76 95 20, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: -0.24 -0.28 Trimming A by 95 22 160 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.28 -0.04 (2nd move error -0.04 0.21) Finished aligning to item in 2 rounds Trimming A by 105 21 160 20, reference by 95 22 160 20 3.3 8.9 unbinned pixels; 28.4 25.3 nm along two shift axes; -35.3 10.9 nm on specimen axes Total_Shift = 18.98991 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.82 microns changed by -4.22 to target drift = 0.20 nm/sec Measured defocus = -1.46 microns changed by 0.06 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1124.tif 27 frames are being saved to U:\krios\20190717\PilA_1125.tif 27 frames are being saved to U:\krios\20190717\PilA_1126.tif 27 frames are being saved to U:\krios\20190717\PilA_1127.tif 27 frames are being saved to U:\krios\20190717\PilA_1128.tif 27 frames are being saved to U:\krios\20190717\PilA_1129.tif Running script 17 at item 257-1 (472.31, -428.41) Remaining time to fill dewars is 19920 Dewars are NOT busy filling Trimming A by 230 21 145 20, reference by 270 20 20 270 Trimming A by 230 21 145 20, reference by 210 21 135 20 Trimming A by 230 21 145 20, reference by 20 136 20 205 Trimming A by 230 21 145 20, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: -0.26 -0.27 Trimming A by 110 24 120 20, reference by 210 21 135 20 Disparity in stage position after aligning to target position: -0.21 -0.12 (2nd move error 0.05 0.12) Finished aligning to item in 2 rounds Trimming A by 115 24 125 20, reference by 110 24 120 20 -0.6 -1.2 unbinned pixels; 3.4 4.0 nm along two shift axes; 4.6 -2.2 nm on specimen axes Total_Shift = 2.634297 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.22 microns changed by -2.72 to target drift = 0.10 nm/sec Measured defocus = -1.49 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1130.tif 27 frames are being saved to U:\krios\20190717\PilA_1131.tif 27 frames are being saved to U:\krios\20190717\PilA_1132.tif 27 frames are being saved to U:\krios\20190717\PilA_1133.tif 27 frames are being saved to U:\krios\20190717\PilA_1134.tif 27 frames are being saved to U:\krios\20190717\PilA_1135.tif Running script 17 at item 258-1 (469.86, -426.75) Remaining time to fill dewars is 19740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 125 21 105 20, reference by 210 21 135 20 Disparity in stage position after aligning to target position: -0.19 -0.29 (2nd move error 0.07 -0.05) Finished aligning to item in 1 rounds Trimming A by 125 21 105 20, reference by 125 21 105 20 -5.7 -3.1 unbinned pixels; 4.0 25.2 nm along two shift axes; 13.3 -21.5 nm on specimen axes Total_Shift = 12.966653 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.36 microns changed by -3.96 to target drift = 0.13 nm/sec Measured defocus = -1.66 microns changed by 0.06 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1136.tif 27 frames are being saved to U:\krios\20190717\PilA_1137.tif 27 frames are being saved to U:\krios\20190717\PilA_1138.tif 27 frames are being saved to U:\krios\20190717\PilA_1139.tif 27 frames are being saved to U:\krios\20190717\PilA_1140.tif 27 frames are being saved to U:\krios\20190717\PilA_1141.tif Running script 17 at item 259 (467.34, -425.01) Remaining time to fill dewars is 19500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 24 90 30, reference by 210 21 135 20 Disparity in stage position after aligning to target position: -0.24 -0.36 (2nd move error 0.03 -0.12) Finished aligning to item in 1 rounds Trimming A by 145 24 85 30, reference by 145 24 90 30 -14.6 -1.7 unbinned pixels; 12.9 55.2 nm along two shift axes; 9.6 -56.5 nm on specimen axes Total_Shift = 29.440709 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.83 microns changed by -2.53 to target drift = 0.13 nm/sec Measured defocus = -1.70 microns changed by -0.00 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1142.tif 27 frames are being saved to U:\krios\20190717\PilA_1143.tif 27 frames are being saved to U:\krios\20190717\PilA_1144.tif 27 frames are being saved to U:\krios\20190717\PilA_1145.tif 27 frames are being saved to U:\krios\20190717\PilA_1146.tif 27 frames are being saved to U:\krios\20190717\PilA_1147.tif Running script 17 at item 260 (464.81, -423.25) Remaining time to fill dewars is 19320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 199 20 20 115, reference by 210 21 135 20 Disparity in stage position after aligning to target position: -0.26 -0.46 (2nd move error -0.00 -0.22) Finished aligning to item in 1 rounds Trimming A by 200 20 20 110, reference by 199 20 20 115 -25.9 2.3 unbinned pixels; 42.1 89.7 nm along two shift axes; -3.4 -101.1 nm on specimen axes Total_Shift = 51.936149 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.86 microns changed by -3.66 to target drift = 0.06 nm/sec Measured defocus = -1.80 microns changed by -0.00 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1148.tif 27 frames are being saved to U:\krios\20190717\PilA_1149.tif 27 frames are being saved to U:\krios\20190717\PilA_1150.tif 27 frames are being saved to U:\krios\20190717\PilA_1151.tif 27 frames are being saved to U:\krios\20190717\PilA_1152.tif 27 frames are being saved to U:\krios\20190717\PilA_1153.tif Running script 17 at item 261 (463.17, -426.27) Remaining time to fill dewars is 19140 Dewars are NOT busy filling Trimming A by 280 20 20 285, reference by 270 20 20 270 Trimming A by 280 20 20 285, reference by 210 21 135 20 Trimming A by 280 20 20 285, reference by 20 136 20 205 Trimming A by 280 20 20 285, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.26 Trimming A by 210 20 110 60, reference by 270 20 20 270 Disparity in stage position after aligning to target position: 0.23 -1.07 (2nd move error 0.05 -0.83) Finished aligning to item in 2 rounds Trimming A by 203 20 110 45, reference by 210 20 110 60 -38.1 -6.1 unbinned pixels; 27.5 146.5 nm along two shift axes; 31.7 -147.1 nm on specimen axes Total_Shift = 77.258161 Trimming A by 206 20 110 35, reference by 210 20 110 60 4.3 -2.4 unbinned pixels; 14.4 12.0 nm along two shift axes; 8.4 17.3 nm on specimen axes Total_Shift = 9.862349 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.45 microns changed by -2.35 to target drift = 0.13 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1154.tif 27 frames are being saved to U:\krios\20190717\PilA_1155.tif 27 frames are being saved to U:\krios\20190717\PilA_1156.tif 27 frames are being saved to U:\krios\20190717\PilA_1157.tif 27 frames are being saved to U:\krios\20190717\PilA_1158.tif 27 frames are being saved to U:\krios\20190717\PilA_1159.tif Running script 17 at item 262 (465.70, -427.55) Remaining time to fill dewars is 18900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 61 20 75 95, reference by 210 21 135 20 Disparity in stage position after aligning to target position: -0.15 -0.39 (2nd move error 0.11 -0.15) Finished aligning to item in 1 rounds Trimming A by 55 20 70 95, reference by 61 20 75 95 -15.0 -1.0 unbinned pixels; 15.9 55.4 nm along two shift axes; 7.0 -58.0 nm on specimen axes Total_Shift = 30.011671 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.69 microns changed by -3.69 to target drift = 0.11 nm/sec Measured defocus = -2.03 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1160.tif 27 frames are being saved to U:\krios\20190717\PilA_1161.tif 27 frames are being saved to U:\krios\20190717\PilA_1162.tif 27 frames are being saved to U:\krios\20190717\PilA_1163.tif 27 frames are being saved to U:\krios\20190717\PilA_1164.tif 27 frames are being saved to U:\krios\20190717\PilA_1165.tif Running script 17 at item 263 (468.22, -429.29) Remaining time to fill dewars is 18780 Dewars are NOT busy filling Trimming A by 225 23 145 20, reference by 270 20 20 270 Trimming A by 225 23 145 20, reference by 210 21 135 20 Trimming A by 225 23 145 20, reference by 20 136 20 205 Trimming A by 225 23 145 20, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.19 Trimming A by 55 20 40 20, reference by 210 21 135 20 Disparity in stage position after aligning to target position: -0.16 -0.30 (2nd move error 0.07 -0.13) Finished aligning to item in 2 rounds Trimming A by 50 20 40 20, reference by 55 20 40 20 -13.8 -6.2 unbinned pixels; 4.9 59.2 nm along two shift axes; 27.2 -52.5 nm on specimen axes Total_Shift = 30.340068 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.42 microns changed by -2.52 to target drift = 0.18 nm/sec Measured defocus = -2.11 microns changed by 0.01 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1166.tif 27 frames are being saved to U:\krios\20190717\PilA_1167.tif 27 frames are being saved to U:\krios\20190717\PilA_1168.tif 27 frames are being saved to U:\krios\20190717\PilA_1169.tif 27 frames are being saved to U:\krios\20190717\PilA_1170.tif 27 frames are being saved to U:\krios\20190717\PilA_1171.tif Running script 17 at item 264 (470.81, -430.88) Remaining time to fill dewars is 18540 Dewars are NOT busy filling Trimming A by 20 80 90 20, reference by 270 20 20 270 Trimming A by 20 80 90 20, reference by 210 21 135 20 Trimming A by 20 80 90 20, reference by 20 136 20 205 Trimming A by 20 80 90 20, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: -0.19 -0.28 Trimming A by 50 20 55 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.25 -0.33 (2nd move error -0.06 -0.08) Finished aligning to item in 2 rounds Trimming A by 35 23 65 20, reference by 50 20 55 20 -10.1 12.1 unbinned pixels; 57.9 18.2 nm along two shift axes; -45.1 -42.0 nm on specimen axes Total_Shift = 31.622561 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.71 microns changed by -3.91 to target drift = 0.07 nm/sec Measured defocus = -2.16 microns changed by -0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1172.tif 27 frames are being saved to U:\krios\20190717\PilA_1173.tif 27 frames are being saved to U:\krios\20190717\PilA_1174.tif 27 frames are being saved to U:\krios\20190717\PilA_1175.tif 27 frames are being saved to U:\krios\20190717\PilA_1176.tif 27 frames are being saved to U:\krios\20190717\PilA_1177.tif Running script 17 at item 265 (473.31, -432.61) Remaining time to fill dewars is 18420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 26 135 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.20 -0.13 (2nd move error -0.01 0.13) Finished aligning to item in 1 rounds Trimming A by 23 29 130 20, reference by 24 26 135 20 -0.9 3.7 unbinned pixels; 14.9 2.4 nm along two shift axes; -14.3 -4.3 nm on specimen axes Total_Shift = 7.678258 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.51 microns changed by -2.81 to target drift = 0.10 nm/sec Measured defocus = -2.39 microns changed by 0.09 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1178.tif 27 frames are being saved to U:\krios\20190717\PilA_1179.tif 27 frames are being saved to U:\krios\20190717\PilA_1180.tif 27 frames are being saved to U:\krios\20190717\PilA_1181.tif 27 frames are being saved to U:\krios\20190717\PilA_1182.tif 27 frames are being saved to U:\krios\20190717\PilA_1183.tif Running script 17 at item 266 (475.81, -434.38) Remaining time to fill dewars is 18120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 98 205 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.23 -0.02 (2nd move error -0.04 0.24) Finished aligning to item in 1 rounds Trimming A by 21 93 205 20, reference by 24 98 205 20 4.3 8.5 unbinned pixels; 25.7 28.5 nm along two shift axes; -34.1 15.1 nm on specimen axes Total_Shift = 19.149434 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.53 microns changed by -2.93 to target drift = 0.16 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1184.tif 27 frames are being saved to U:\krios\20190717\PilA_1185.tif 27 frames are being saved to U:\krios\20190717\PilA_1186.tif 27 frames are being saved to U:\krios\20190717\PilA_1187.tif 27 frames are being saved to U:\krios\20190717\PilA_1188.tif 27 frames are being saved to U:\krios\20190717\PilA_1189.tif Running script 17 at item 267 (474.15, -436.95) Remaining time to fill dewars is 18000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 110 190 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.10 -0.12 (2nd move error 0.09 0.14) Finished aligning to item in 1 rounds Trimming A by 23 118 185 20, reference by 21 110 190 20 0.0 -0.4 unbinned pixels; 1.5 0.5 nm along two shift axes; 1.6 0.2 nm on specimen axes Total_Shift = 0.814164 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.26 microns changed by -2.76 to target drift = 0.11 nm/sec Measured defocus = -2.51 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1190.tif 27 frames are being saved to U:\krios\20190717\PilA_1191.tif 27 frames are being saved to U:\krios\20190717\PilA_1192.tif 27 frames are being saved to U:\krios\20190717\PilA_1193.tif 27 frames are being saved to U:\krios\20190717\PilA_1194.tif 27 frames are being saved to U:\krios\20190717\PilA_1195.tif Running script 17 at item 268 (471.67, -435.17) Remaining time to fill dewars is 17820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 66 100 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.16 -0.33 (2nd move error 0.03 -0.07) Finished aligning to item in 1 rounds Trimming A by 24 53 110 20, reference by 20 66 100 20 -10.1 -3.3 unbinned pixels; 1.1 41.1 nm along two shift axes; 14.8 -38.4 nm on specimen axes Total_Shift = 21.141319 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.12 microns changed by -2.72 to target drift = 0.12 nm/sec Measured defocus = -2.61 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1196.tif 27 frames are being saved to U:\krios\20190717\PilA_1197.tif 27 frames are being saved to U:\krios\20190717\PilA_1198.tif 27 frames are being saved to U:\krios\20190717\PilA_1199.tif 27 frames are being saved to U:\krios\20190717\PilA_1200.tif 27 frames are being saved to U:\krios\20190717\PilA_1201.tif Running script 17 at item 269 (469.14, -433.45) Remaining time to fill dewars is 17580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.22 -0.46 (2nd move error -0.03 -0.20) Finished aligning to item in 1 rounds -29.7 14.1 unbinned pixels; 90.7 85.8 nm along two shift axes; -48.7 -118.7 nm on specimen axes Total_Shift = 65.831912 -5.4 7.7 unbinned pixels; 35.3 7.9 nm along two shift axes; -28.8 -22.6 nm on specimen axes Total_Shift = 18.799803 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.59 microns changed by -2.69 to target drift = 0.02 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1202.tif 27 frames are being saved to U:\krios\20190717\PilA_1203.tif 27 frames are being saved to U:\krios\20190717\PilA_1204.tif 27 frames are being saved to U:\krios\20190717\PilA_1205.tif 27 frames are being saved to U:\krios\20190717\PilA_1206.tif 27 frames are being saved to U:\krios\20190717\PilA_1207.tif Running script 17 at item 270 (466.66, -431.74) Remaining time to fill dewars is 17340 Dewars are NOT busy filling Trimming A by 24 71 95 20, reference by 270 20 20 270 Trimming A by 24 71 95 20, reference by 210 21 135 20 Trimming A by 24 71 95 20, reference by 20 136 20 205 Trimming A by 24 71 95 20, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.31 Trimming A by 20 20 20 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.24 -0.58 (2nd move error -0.10 -0.30) Finished aligning to item in 2 rounds -26.5 18.0 unbinned pixels; 100.7 68.2 nm along two shift axes; -64.4 -106.7 nm on specimen axes Total_Shift = 64.005487 0.1 10.8 unbinned pixels; 39.6 16.5 nm along two shift axes; -42.0 -2.0 nm on specimen axes Total_Shift = 21.590277 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.74 microns changed by -3.94 to target drift = 0.05 nm/sec Measured defocus = -1.30 microns changed by 0.10 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1208.tif 27 frames are being saved to U:\krios\20190717\PilA_1209.tif 27 frames are being saved to U:\krios\20190717\PilA_1210.tif 27 frames are being saved to U:\krios\20190717\PilA_1211.tif 27 frames are being saved to U:\krios\20190717\PilA_1212.tif 27 frames are being saved to U:\krios\20190717\PilA_1213.tif Running script 17 at item 271 (464.09, -429.98) Remaining time to fill dewars is 17160 Dewars are NOT busy filling Trimming A by 224 22 150 20, reference by 270 20 20 270 Trimming A by 224 22 150 20, reference by 210 21 135 20 Trimming A by 224 22 150 20, reference by 20 136 20 205 Trimming A by 224 22 150 20, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: -0.17 -0.25 Trimming A by 80 21 50 75, reference by 210 21 135 20 Disparity in stage position after aligning to target position: -0.16 -0.55 (2nd move error 0.00 -0.34) Finished aligning to item in 2 rounds Trimming A by 75 21 45 70, reference by 80 21 50 75 -30.6 0.1 unbinned pixels; 40.3 109.8 nm along two shift axes; 6.0 -118.9 nm on specimen axes Total_Shift = 61.126169 Trimming A by 70 20 20 75, reference by 80 21 50 75 -0.6 -2.5 unbinned pixels; 8.3 6.0 nm along two shift axes; 9.8 -2.0 nm on specimen axes Total_Shift = 5.122789 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.13 microns changed by -2.43 to target drift = 0.08 nm/sec Measured defocus = -1.25 microns changed by -0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1214.tif 27 frames are being saved to U:\krios\20190717\PilA_1215.tif 27 frames are being saved to U:\krios\20190717\PilA_1216.tif 27 frames are being saved to U:\krios\20190717\PilA_1217.tif 27 frames are being saved to U:\krios\20190717\PilA_1218.tif 27 frames are being saved to U:\krios\20190717\PilA_1219.tif Running script 17 at item 272 (461.40, -428.93) Remaining time to fill dewars is 16920 Dewars are NOT busy filling Trimming A by 285 21 65 280, reference by 270 20 20 270 Trimming A by 285 21 65 280, reference by 210 21 135 20 Trimming A by 285 21 65 280, reference by 20 136 20 205 Trimming A by 285 21 65 280, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.39 Trimming A by 125 22 20 130, reference by 270 20 20 270 Disparity in stage position after aligning to target position: 0.17 -1.12 (2nd move error 0.00 -0.75) Finished aligning to item in 2 rounds Trimming A by 115 23 20 125, reference by 125 22 20 130 -32.8 -4.0 unbinned pixels; 28.0 123.9 nm along two shift axes; 22.5 -126.6 nm on specimen axes Total_Shift = 65.98902 Trimming A by 110 24 20 125, reference by 125 22 20 130 -0.5 -4.6 unbinned pixels; 16.2 8.9 nm along two shift axes; 18.1 -1.1 nm on specimen axes Total_Shift = 9.288571 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.40 microns changed by -3.80 to target drift = 0.15 nm/sec Measured defocus = -1.52 microns changed by 0.12 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1220.tif 27 frames are being saved to U:\krios\20190717\PilA_1221.tif 27 frames are being saved to U:\krios\20190717\PilA_1222.tif 27 frames are being saved to U:\krios\20190717\PilA_1223.tif 27 frames are being saved to U:\krios\20190717\PilA_1224.tif 27 frames are being saved to U:\krios\20190717\PilA_1225.tif Running script 17 at item 273 (460.08, -431.52) Remaining time to fill dewars is 16680 Dewars are NOT busy filling Trimming A by 23 126 20 215, reference by 270 20 20 270 Trimming A by 23 126 20 215, reference by 210 21 135 20 Trimming A by 23 126 20 215, reference by 20 136 20 205 Trimming A by 23 126 20 215, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: -0.16 -0.23 Trimming A by 100 22 20 145, reference by 20 136 20 205 Disparity in stage position after aligning to target position: -0.15 -0.12 (2nd move error 0.01 0.09) Finished aligning to item in 2 rounds Trimming A by 92 23 20 155, reference by 100 22 20 145 -2.2 0.3 unbinned pixels; 3.9 7.6 nm along two shift axes; -0.6 -8.8 nm on specimen axes Total_Shift = 4.508815 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.81 microns changed by -2.31 to target drift = 0.11 nm/sec Measured defocus = -1.49 microns changed by -0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1226.tif 27 frames are being saved to U:\krios\20190717\PilA_1227.tif 27 frames are being saved to U:\krios\20190717\PilA_1228.tif 27 frames are being saved to U:\krios\20190717\PilA_1229.tif 27 frames are being saved to U:\krios\20190717\PilA_1230.tif 27 frames are being saved to U:\krios\20190717\PilA_1231.tif Running script 17 at item 274 (462.61, -433.25) Remaining time to fill dewars is 16560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 22 20 80, reference by 20 136 20 205 Disparity in stage position after aligning to target position: -0.12 -0.02 (2nd move error 0.04 0.19) Finished aligning to item in 1 rounds Trimming A by 31 21 20 75, reference by 22 22 20 80 0.4 -0.8 unbinned pixels; 3.5 0.3 nm along two shift axes; 3.0 1.8 nm on specimen axes Total_Shift = 1.813474 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.09 microns changed by -3.69 to target drift = 0.15 nm/sec Measured defocus = -1.58 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1232.tif 27 frames are being saved to U:\krios\20190717\PilA_1233.tif 27 frames are being saved to U:\krios\20190717\PilA_1234.tif 27 frames are being saved to U:\krios\20190717\PilA_1235.tif 27 frames are being saved to U:\krios\20190717\PilA_1236.tif 27 frames are being saved to U:\krios\20190717\PilA_1237.tif Running script 17 at item 275 (464.98, -434.31) Remaining time to fill dewars is 16440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 30 20 45, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.07 -0.61 (2nd move error 0.07 -0.33) Finished aligning to item in 1 rounds Trimming A by 21 35 20 45, reference by 21 30 20 45 -26.6 -7.1 unbinned pixels; 8.8 106.5 nm along two shift axes; 33.1 -102.1 nm on specimen axes Total_Shift = 55.084027 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.81 microns changed by -2.51 to target drift = 0.22 nm/sec Measured defocus = -1.73 microns changed by 0.03 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1238.tif 27 frames are being saved to U:\krios\20190717\PilA_1239.tif 27 frames are being saved to U:\krios\20190717\PilA_1240.tif 27 frames are being saved to U:\krios\20190717\PilA_1241.tif 27 frames are being saved to U:\krios\20190717\PilA_1242.tif 27 frames are being saved to U:\krios\20190717\PilA_1243.tif Running script 17 at item 276 (467.54, -436.05) Remaining time to fill dewars is 16140 Dewars are NOT busy filling Trimming A by 21 80 90 20, reference by 270 20 20 270 Trimming A by 21 80 90 20, reference by 210 21 135 20 Trimming A by 21 80 90 20, reference by 20 136 20 205 Trimming A by 21 80 90 20, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: -0.17 -0.24 Trimming A by 21 35 20 35, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.15 -0.48 (2nd move error 0.02 -0.26) Finished aligning to item in 2 rounds Trimming A by 21 35 20 40, reference by 21 35 20 35 -28.8 -1.8 unbinned pixels; 31.1 106.2 nm along two shift axes; 12.9 -111.6 nm on specimen axes Total_Shift = 57.650146 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 2.06 microns changed by -3.86 to target drift = 0.12 nm/sec Measured defocus = -1.76 microns changed by -0.04 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1244.tif 27 frames are being saved to U:\krios\20190717\PilA_1245.tif 27 frames are being saved to U:\krios\20190717\PilA_1246.tif 27 frames are being saved to U:\krios\20190717\PilA_1247.tif 27 frames are being saved to U:\krios\20190717\PilA_1248.tif 27 frames are being saved to U:\krios\20190717\PilA_1249.tif Running script 17 at item 277 (470.04, -437.82) Remaining time to fill dewars is 15960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 95 80 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.07 -0.32 (2nd move error 0.11 -0.11) Finished aligning to item in 1 rounds Trimming A by 22 100 80 20, reference by 22 95 80 20 -8.5 -1.4 unbinned pixels; 5.9 32.9 nm along two shift axes; 7.3 -32.9 nm on specimen axes Total_Shift = 17.323205 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.82 microns changed by -2.72 to target drift = 0.11 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1250.tif 27 frames are being saved to U:\krios\20190717\PilA_1251.tif 27 frames are being saved to U:\krios\20190717\PilA_1252.tif 27 frames are being saved to U:\krios\20190717\PilA_1253.tif 27 frames are being saved to U:\krios\20190717\PilA_1254.tif 27 frames are being saved to U:\krios\20190717\PilA_1255.tif Running script 17 at item 278 (472.53, -439.53) Remaining time to fill dewars is 15840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 160 150 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.10 -0.18 (2nd move error 0.07 0.03) Finished aligning to item in 1 rounds Trimming A by 23 152 160 20, reference by 20 160 150 20 -1.9 -2.6 unbinned pixels; 7.1 10.8 nm along two shift axes; 10.5 -6.9 nm on specimen axes Total_Shift = 6.445829 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 2.13 microns changed by -4.13 to target drift = 0.12 nm/sec Measured defocus = -2.00 microns changed by -0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1256.tif 27 frames are being saved to U:\krios\20190717\PilA_1257.tif 27 frames are being saved to U:\krios\20190717\PilA_1258.tif 27 frames are being saved to U:\krios\20190717\PilA_1259.tif 27 frames are being saved to U:\krios\20190717\PilA_1260.tif 27 frames are being saved to U:\krios\20190717\PilA_1261.tif Running script 17 at item 279 (470.80, -442.06) Remaining time to fill dewars is 15600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 199 125 20, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.05 -0.28 (2nd move error 0.12 -0.06) Finished aligning to item in 1 rounds Trimming A by 22 205 120 20, reference by 23 199 125 20 -4.8 -4.4 unbinned pixels; 10.0 24.0 nm along two shift axes; 18.3 -17.8 nm on specimen axes Total_Shift = 13.090996 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.70 microns changed by -2.80 to target drift = 0.15 nm/sec Measured defocus = -2.10 microns changed by -0.00 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1262.tif 27 frames are being saved to U:\krios\20190717\PilA_1263.tif 27 frames are being saved to U:\krios\20190717\PilA_1264.tif 27 frames are being saved to U:\krios\20190717\PilA_1265.tif 27 frames are being saved to U:\krios\20190717\PilA_1266.tif 27 frames are being saved to U:\krios\20190717\PilA_1267.tif Running script 17 at item 280 (468.34, -440.36) Remaining time to fill dewars is 15420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 130 50 85, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.02 -0.44 (2nd move error 0.15 -0.21) Finished aligning to item in 1 rounds Trimming A by 21 135 75 80, reference by 20 130 50 85 -21.0 -3.5 unbinned pixels; 14.4 80.9 nm along two shift axes; 18.2 -80.9 nm on specimen axes Total_Shift = 42.579109 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.47 microns changed by -2.67 to target drift = 0.16 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1268.tif 27 frames are being saved to U:\krios\20190717\PilA_1269.tif 27 frames are being saved to U:\krios\20190717\PilA_1270.tif 27 frames are being saved to U:\krios\20190717\PilA_1271.tif 27 frames are being saved to U:\krios\20190717\PilA_1272.tif 27 frames are being saved to U:\krios\20190717\PilA_1273.tif Running script 17 at item 281 (465.83, -438.56) Remaining time to fill dewars is 15240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 105 20 95, reference by 23 62 95 20 Disparity in stage position after aligning to target position: -0.03 -0.57 (2nd move error 0.14 -0.35) Finished aligning to item in 1 rounds Trimming A by 21 115 65 90, reference by 23 105 20 95 -27.5 -3.0 unbinned pixels; 25.0 103.6 nm along two shift axes; 17.3 -106.5 nm on specimen axes Total_Shift = 55.368669 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.55 microns changed by -3.85 to target drift = 0.15 nm/sec Measured defocus = -2.35 microns changed by 0.05 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1274.tif 27 frames are being saved to U:\krios\20190717\PilA_1275.tif 27 frames are being saved to U:\krios\20190717\PilA_1276.tif 27 frames are being saved to U:\krios\20190717\PilA_1277.tif 27 frames are being saved to U:\krios\20190717\PilA_1278.tif 27 frames are being saved to U:\krios\20190717\PilA_1279.tif Running script 17 at item 282 (463.28, -437.43) Remaining time to fill dewars is 15060 Dewars are NOT busy filling Trimming A by 21 131 20 210, reference by 270 20 20 270 Trimming A by 21 131 20 210, reference by 210 21 135 20 Trimming A by 21 131 20 210, reference by 20 136 20 205 Trimming A by 21 131 20 210, reference by 23 62 95 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.20 Trimming A by 20 84 20 135, reference by 20 136 20 205 Disparity in stage position after aligning to target position: -0.07 0.09 (2nd move error 0.12 0.26) Finished aligning to item in 2 rounds Trimming A by 24 91 20 120, reference by 20 84 20 135 4.4 1.1 unbinned pixels; 1.7 17.4 nm along two shift axes; -5.2 16.8 nm on specimen axes Total_Shift = 9.032873 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.00 microns changed by -2.40 to target drift = 0.12 nm/sec Measured defocus = -2.45 microns changed by 0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1280.tif 27 frames are being saved to U:\krios\20190717\PilA_1281.tif 27 frames are being saved to U:\krios\20190717\PilA_1282.tif 27 frames are being saved to U:\krios\20190717\PilA_1283.tif 27 frames are being saved to U:\krios\20190717\PilA_1284.tif 27 frames are being saved to U:\krios\20190717\PilA_1285.tif Running script 17 at item 283 (460.90, -435.84) Remaining time to fill dewars is 14820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 95 20 130, reference by 20 136 20 205 Disparity in stage position after aligning to target position: -0.10 -0.11 (2nd move error 0.09 0.07) Finished aligning to item in 1 rounds Trimming A by 20 95 20 125, reference by 21 95 20 130 -1.8 -1.5 unbinned pixels; 3.0 8.8 nm along two shift axes; 6.1 -6.8 nm on specimen axes Total_Shift = 4.677804 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.10 microns changed by -3.60 to target drift = 0.11 nm/sec Measured defocus = -2.51 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1286.tif 27 frames are being saved to U:\krios\20190717\PilA_1287.tif 27 frames are being saved to U:\krios\20190717\PilA_1288.tif 27 frames are being saved to U:\krios\20190717\PilA_1289.tif 27 frames are being saved to U:\krios\20190717\PilA_1290.tif 27 frames are being saved to U:\krios\20190717\PilA_1291.tif Running script 17 at item 284 (458.41, -434.10) Remaining time to fill dewars is 14580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 75 20 170, reference by 20 136 20 205 Disparity in stage position after aligning to target position: -0.17 -0.20 (2nd move error 0.02 -0.03) Finished aligning to item in 1 rounds Trimming A by 70 75 20 170, reference by 70 75 20 170 -3.5 -1.6 unbinned pixels; 1.2 15.1 nm along two shift axes; 6.9 -13.4 nm on specimen axes Total_Shift = 7.722563 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.45 microns changed by -2.15 to target drift = 0.09 nm/sec Measured defocus = -2.59 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1292.tif 27 frames are being saved to U:\krios\20190717\PilA_1293.tif 27 frames are being saved to U:\krios\20190717\PilA_1294.tif 27 frames are being saved to U:\krios\20190717\PilA_1295.tif 27 frames are being saved to U:\krios\20190717\PilA_1296.tif 27 frames are being saved to U:\krios\20190717\PilA_1297.tif Running script 17 at item 285 (505.66, -373.68) Remaining time to fill dewars is 14400 Dewars are NOT busy filling Trimming A by 190 24 180 20, reference by 162 22 20 200 Trimming A by 190 24 180 20, reference by 175 20 165 20 Trimming A by 190 24 180 20, reference by 20 220 20 205 Trimming A by 190 24 180 20, reference by 20 196 180 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.26 Trimming A by 121 21 210 20, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.19 -0.08 (2nd move error 0.12 0.16) Finished aligning to item in 2 rounds Trimming A by 130 24 210 20, reference by 121 21 210 20 -1.5 -1.1 unbinned pixels; 2.0 6.9 nm along two shift axes; 4.5 -5.5 nm on specimen axes Total_Shift = 3.653556 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.92 microns changed by -4.02 to target drift = 0.16 nm/sec Measured defocus = -1.15 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1298.tif 27 frames are being saved to U:\krios\20190717\PilA_1299.tif 27 frames are being saved to U:\krios\20190717\PilA_1300.tif 27 frames are being saved to U:\krios\20190717\PilA_1301.tif 27 frames are being saved to U:\krios\20190717\PilA_1302.tif 27 frames are being saved to U:\krios\20190717\PilA_1303.tif Running script 17 at item 286 (503.23, -371.99) Remaining time to fill dewars is 14220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 24 165 20, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.25 -0.23 (2nd move error 0.05 0.00) Finished aligning to item in 1 rounds Trimming A by 150 24 165 20, reference by 150 24 165 20 -0.4 -3.4 unbinned pixels; 11.9 6.5 nm along two shift axes; 13.2 -0.8 nm on specimen axes Total_Shift = 6.814119 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.38 microns changed by -2.58 to target drift = 0.13 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1304.tif 27 frames are being saved to U:\krios\20190717\PilA_1305.tif 27 frames are being saved to U:\krios\20190717\PilA_1306.tif 27 frames are being saved to U:\krios\20190717\PilA_1307.tif 27 frames are being saved to U:\krios\20190717\PilA_1308.tif 27 frames are being saved to U:\krios\20190717\PilA_1309.tif Running script 17 at item 287 (500.73, -370.28) Remaining time to fill dewars is 14040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 175 20 135 20, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.21 -0.36 (2nd move error 0.10 -0.12) Finished aligning to item in 1 rounds Trimming A by 170 20 140 20, reference by 175 20 135 20 -13.5 -1.2 unbinned pixels; 13.4 50.4 nm along two shift axes; 7.4 -52.3 nm on specimen axes Total_Shift = 27.123592 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.47 microns changed by -3.77 to target drift = 0.09 nm/sec Measured defocus = -1.33 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1310.tif 27 frames are being saved to U:\krios\20190717\PilA_1311.tif 27 frames are being saved to U:\krios\20190717\PilA_1312.tif 27 frames are being saved to U:\krios\20190717\PilA_1313.tif 27 frames are being saved to U:\krios\20190717\PilA_1314.tif 27 frames are being saved to U:\krios\20190717\PilA_1315.tif Running script 17 at item 288 (498.17, -368.55) Remaining time to fill dewars is 13860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 195 24 115 20, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.25 -0.45 (2nd move error 0.06 -0.22) Finished aligning to item in 1 rounds Trimming A by 195 22 115 20, reference by 195 24 115 20 -26.1 -1.3 unbinned pixels; 29.4 95.9 nm along two shift axes; 10.4 -101.3 nm on specimen axes Total_Shift = 52.252663 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.92 microns changed by -2.32 to target drift = 0.16 nm/sec Measured defocus = -1.51 microns changed by 0.11 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1316.tif 27 frames are being saved to U:\krios\20190717\PilA_1317.tif 27 frames are being saved to U:\krios\20190717\PilA_1318.tif 27 frames are being saved to U:\krios\20190717\PilA_1319.tif 27 frames are being saved to U:\krios\20190717\PilA_1320.tif 27 frames are being saved to U:\krios\20190717\PilA_1321.tif Running script 17 at item 289 (496.56, -371.09) Remaining time to fill dewars is 13680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 110 20 70 60, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.16 -0.59 (2nd move error 0.15 -0.35) Finished aligning to item in 1 rounds Trimming A by 110 20 55 45, reference by 110 20 70 60 -17.1 -4.4 unbinned pixels; 6.2 68.0 nm along two shift axes; 20.6 -65.5 nm on specimen axes Total_Shift = 35.242027 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.11 microns changed by -3.61 to target drift = 0.13 nm/sec Measured defocus = -1.51 microns changed by 0.01 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1322.tif 27 frames are being saved to U:\krios\20190717\PilA_1323.tif 27 frames are being saved to U:\krios\20190717\PilA_1324.tif 27 frames are being saved to U:\krios\20190717\PilA_1325.tif 27 frames are being saved to U:\krios\20190717\PilA_1326.tif 27 frames are being saved to U:\krios\20190717\PilA_1327.tif Running script 17 at item 290 (499.02, -372.84) Remaining time to fill dewars is 13500 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 95 22 75 20, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.17 -0.43 (2nd move error 0.14 -0.19) Finished aligning to item in 1 rounds Trimming A by 95 22 75 20, reference by 95 22 75 20 -21.3 -3.8 unbinned pixels; 13.8 82.5 nm along two shift axes; 19.3 -82.1 nm on specimen axes Total_Shift = 43.311953 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.73 microns changed by -2.33 to target drift = 0.14 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1328.tif 27 frames are being saved to U:\krios\20190717\PilA_1329.tif 27 frames are being saved to U:\krios\20190717\PilA_1330.tif 27 frames are being saved to U:\krios\20190717\PilA_1331.tif 27 frames are being saved to U:\krios\20190717\PilA_1332.tif 27 frames are being saved to U:\krios\20190717\PilA_1333.tif Running script 17 at item 291 (501.58, -374.56) Remaining time to fill dewars is 13260 Dewars are NOT busy filling Trimming A by 190 24 180 20, reference by 162 22 20 200 Trimming A by 190 24 180 20, reference by 175 20 165 20 Trimming A by 190 24 180 20, reference by 20 220 20 205 Trimming A by 190 24 180 20, reference by 20 196 180 20 Disparity in stage position after aligning to center of map frame: -0.24 -0.22 Trimming A by 80 20 95 20, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.07 -0.30 (2nd move error 0.17 -0.10) Finished aligning to item in 2 rounds Trimming A by 80 20 95 20, reference by 80 20 95 20 -9.1 -0.9 unbinned pixels; 8.6 34.3 nm along two shift axes; 5.4 -35.4 nm on specimen axes Total_Shift = 18.366109 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 2.17 microns changed by -3.87 to target drift = 0.07 nm/sec Measured defocus = -1.78 microns changed by 0.08 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1334.tif 27 frames are being saved to U:\krios\20190717\PilA_1335.tif 27 frames are being saved to U:\krios\20190717\PilA_1336.tif 27 frames are being saved to U:\krios\20190717\PilA_1337.tif 27 frames are being saved to U:\krios\20190717\PilA_1338.tif 27 frames are being saved to U:\krios\20190717\PilA_1339.tif Running script 17 at item 292 (504.03, -376.24) Remaining time to fill dewars is 13020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 50 56 145 20, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.03 -0.15 (2nd move error 0.21 0.04) Finished aligning to item in 1 rounds Trimming A by 42 45 160 20, reference by 50 56 145 20 0.2 -6.0 unbinned pixels; 22.5 8.4 nm along two shift axes; 23.5 2.1 nm on specimen axes Total_Shift = 12.105953 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.75 microns changed by -2.55 to target drift = 0.18 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1340.tif 27 frames are being saved to U:\krios\20190717\PilA_1341.tif 27 frames are being saved to U:\krios\20190717\PilA_1342.tif 27 frames are being saved to U:\krios\20190717\PilA_1343.tif 27 frames are being saved to U:\krios\20190717\PilA_1344.tif 27 frames are being saved to U:\krios\20190717\PilA_1345.tif Running script 17 at item 293 (502.67, -378.77) Remaining time to fill dewars is 12960 Dewars are NOT busy filling Trimming A by 20 220 180 20, reference by 162 22 20 200 Trimming A by 20 220 180 20, reference by 175 20 165 20 Trimming A by 20 220 180 20, reference by 20 220 20 205 Trimming A by 20 220 180 20, reference by 20 196 180 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.25 Trimming A by 22 78 140 20, reference by 20 196 180 20 Disparity in stage position after aligning to target position: -0.36 -0.32 (2nd move error -0.16 -0.08) Finished aligning to item in 2 rounds Trimming A by 24 66 145 20, reference by 22 78 140 20 -6.1 29.3 unbinned pixels; 115.7 22.3 nm along two shift axes; -112.8 -29.8 nm on specimen axes Total_Shift = 59.888033 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.90 microns changed by -3.80 to target drift = 0.06 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1346.tif 27 frames are being saved to U:\krios\20190717\PilA_1347.tif 27 frames are being saved to U:\krios\20190717\PilA_1348.tif 27 frames are being saved to U:\krios\20190717\PilA_1349.tif 27 frames are being saved to U:\krios\20190717\PilA_1350.tif 27 frames are being saved to U:\krios\20190717\PilA_1351.tif Running script 17 at item 294 (499.96, -377.08) Remaining time to fill dewars is 12660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 21 55 20, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.02 -0.39 (2nd move error 0.22 -0.20) Finished aligning to item in 1 rounds Trimming A by 21 21 60 20, reference by 21 21 55 20 -21.0 -3.9 unbinned pixels; 12.9 81.4 nm along two shift axes; 19.7 -80.8 nm on specimen axes Total_Shift = 42.676848 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.32 microns changed by -2.32 to target drift = 0.20 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1352.tif 27 frames are being saved to U:\krios\20190717\PilA_1353.tif 27 frames are being saved to U:\krios\20190717\PilA_1354.tif 27 frames are being saved to U:\krios\20190717\PilA_1355.tif 27 frames are being saved to U:\krios\20190717\PilA_1356.tif 27 frames are being saved to U:\krios\20190717\PilA_1357.tif Running script 17 at item 295 (497.50, -375.34) Remaining time to fill dewars is 12540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 25 20 20 20, reference by 175 20 165 20 Disparity in stage position after aligning to target position: -0.14 -0.53 (2nd move error 0.11 -0.33) Finished aligning to item in 1 rounds Trimming A by 20 20 20 20, reference by 25 20 20 20 -20.9 -5.8 unbinned pixels; 6.0 84.0 nm along two shift axes; 27.0 -80.2 nm on specimen axes Total_Shift = 43.420104 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.65 microns changed by -3.75 to target drift = 0.18 nm/sec Measured defocus = -2.22 microns changed by 0.12 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1358.tif 27 frames are being saved to U:\krios\20190717\PilA_1359.tif 27 frames are being saved to U:\krios\20190717\PilA_1360.tif 27 frames are being saved to U:\krios\20190717\PilA_1361.tif 27 frames are being saved to U:\krios\20190717\PilA_1362.tif 27 frames are being saved to U:\krios\20190717\PilA_1363.tif Running script 17 at item 296 (494.84, -374.25) Remaining time to fill dewars is 12240 Dewars are NOT busy filling Trimming A by 172 21 20 205, reference by 162 22 20 200 Trimming A by 172 21 20 205, reference by 175 20 165 20 Trimming A by 172 21 20 205, reference by 20 220 20 205 Trimming A by 172 21 20 205, reference by 20 196 180 20 Disparity in stage position after aligning to center of map frame: -0.18 -0.19 Trimming A by 65 20 20 65, reference by 162 22 20 200 Disparity in stage position after aligning to target position: -0.07 0.08 (2nd move error 0.10 0.24) Finished aligning to item in 2 rounds Trimming A by 56 24 20 75, reference by 65 20 20 65 2.3 0.4 unbinned pixels; 1.5 8.7 nm along two shift axes; -2.0 8.7 nm on specimen axes Total_Shift = 4.577039 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.01 microns changed by -2.19 to target drift = 0.12 nm/sec Measured defocus = -2.17 microns changed by -0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1364.tif 27 frames are being saved to U:\krios\20190717\PilA_1365.tif 27 frames are being saved to U:\krios\20190717\PilA_1366.tif 27 frames are being saved to U:\krios\20190717\PilA_1367.tif 27 frames are being saved to U:\krios\20190717\PilA_1368.tif 27 frames are being saved to U:\krios\20190717\PilA_1369.tif Running script 17 at item 297 (492.39, -372.54) Remaining time to fill dewars is 12180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 24 20 140, reference by 162 22 20 200 Disparity in stage position after aligning to target position: -0.06 -0.11 (2nd move error 0.11 0.06) Finished aligning to item in 1 rounds Trimming A by 116 20 20 150, reference by 130 24 20 140 -1.6 -4.0 unbinned pixels; 12.7 11.7 nm along two shift axes; 15.9 -5.2 nm on specimen axes Total_Shift = 8.58659 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.06 microns changed by -3.36 to target drift = 0.15 nm/sec Measured defocus = -2.26 microns changed by -0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1370.tif 27 frames are being saved to U:\krios\20190717\PilA_1371.tif 27 frames are being saved to U:\krios\20190717\PilA_1372.tif 27 frames are being saved to U:\krios\20190717\PilA_1373.tif 27 frames are being saved to U:\krios\20190717\PilA_1374.tif 27 frames are being saved to U:\krios\20190717\PilA_1375.tif Running script 17 at item 298 (490.73, -375.09) Remaining time to fill dewars is 11880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 79 23 20 180, reference by 162 22 20 200 Disparity in stage position after aligning to target position: -0.07 -0.22 (2nd move error 0.11 -0.05) Finished aligning to item in 1 rounds Trimming A by 86 23 20 170, reference by 79 23 20 180 -5.7 -1.2 unbinned pixels; 3.0 22.3 nm along two shift axes; 5.9 -21.9 nm on specimen axes Total_Shift = 11.625315 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.34 microns changed by -2.06 to target drift = 0.09 nm/sec Measured defocus = -2.44 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1376.tif 27 frames are being saved to U:\krios\20190717\PilA_1377.tif 27 frames are being saved to U:\krios\20190717\PilA_1378.tif 27 frames are being saved to U:\krios\20190717\PilA_1379.tif 27 frames are being saved to U:\krios\20190717\PilA_1380.tif 27 frames are being saved to U:\krios\20190717\PilA_1381.tif Running script 17 at item 299 (493.29, -376.82) Remaining time to fill dewars is 11760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 23 20 100, reference by 162 22 20 200 Disparity in stage position after aligning to target position: 0.05 -0.02 (2nd move error 0.23 0.15) Finished aligning to item in 1 rounds Trimming A by 31 21 20 90, reference by 23 23 20 100 1.4 2.6 unbinned pixels; 7.6 9.1 nm along two shift axes; -10.3 5.1 nm on specimen axes Total_Shift = 5.895893 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.91 microns changed by -3.41 to target drift = 0.10 nm/sec Measured defocus = -2.47 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1382.tif 27 frames are being saved to U:\krios\20190717\PilA_1383.tif 27 frames are being saved to U:\krios\20190717\PilA_1384.tif 27 frames are being saved to U:\krios\20190717\PilA_1385.tif 27 frames are being saved to U:\krios\20190717\PilA_1386.tif 27 frames are being saved to U:\krios\20190717\PilA_1387.tif Running script 17 at item 300 (496.00, -378.45) Remaining time to fill dewars is 11520 Dewars are NOT busy filling Trimming A by 23 225 20 200, reference by 162 22 20 200 Trimming A by 23 225 20 200, reference by 175 20 165 20 Trimming A by 23 225 20 200, reference by 20 220 20 205 Trimming A by 23 225 20 200, reference by 20 196 180 20 Disparity in stage position after aligning to center of map frame: -0.15 -0.25 Trimming A by 21 40 20 50, reference by 20 220 20 205 Disparity in stage position after aligning to target position: -0.08 0.01 (2nd move error 0.07 0.23) Finished aligning to item in 2 rounds Trimming A by 21 35 20 45, reference by 21 40 20 50 1.6 -1.5 unbinned pixels; 7.5 3.5 nm along two shift axes; 5.4 6.5 nm on specimen axes Total_Shift = 4.353716 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.36 microns changed by -2.24 to target drift = 0.18 nm/sec Measured defocus = -2.57 microns changed by -0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1388.tif 27 frames are being saved to U:\krios\20190717\PilA_1389.tif 27 frames are being saved to U:\krios\20190717\PilA_1390.tif 27 frames are being saved to U:\krios\20190717\PilA_1391.tif 27 frames are being saved to U:\krios\20190717\PilA_1392.tif 27 frames are being saved to U:\krios\20190717\PilA_1393.tif Running script 17 at item 301 (498.57, -379.62) Remaining time to fill dewars is 11400 Dewars are NOT busy filling Trimming A by 20 220 180 20, reference by 162 22 20 200 Trimming A by 20 220 180 20, reference by 175 20 165 20 Trimming A by 20 220 180 20, reference by 20 220 20 205 Trimming A by 20 220 180 20, reference by 20 196 180 20 Disparity in stage position after aligning to center of map frame: -0.19 -0.18 Trimming A by 20 50 35 30, reference by 20 196 180 20 Disparity in stage position after aligning to target position: -0.33 -0.50 (2nd move error -0.15 -0.34) Finished aligning to item in 2 rounds Trimming A by 20 50 40 25, reference by 20 50 35 30 -26.7 43.9 unbinned pixels; 196.2 30.1 nm along two shift axes; -165.1 -113.1 nm on specimen axes Total_Shift = 102.742464 Trimming A by 20 55 40 25, reference by 20 50 35 30 4.6 28.5 unbinned pixels; 98.8 59.6 nm along two shift axes; -111.9 12.0 nm on specimen axes Total_Shift = 57.771849 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.63 microns changed by -3.73 to target drift = 0.05 nm/sec Measured defocus = -1.15 microns changed by 0.05 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1394.tif 27 frames are being saved to U:\krios\20190717\PilA_1395.tif 27 frames are being saved to U:\krios\20190717\PilA_1396.tif 27 frames are being saved to U:\krios\20190717\PilA_1397.tif 27 frames are being saved to U:\krios\20190717\PilA_1398.tif 27 frames are being saved to U:\krios\20190717\PilA_1399.tif Running script 17 at item 302 (501.02, -381.41) Remaining time to fill dewars is 11160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 120 95 20, reference by 20 196 180 20 Disparity in stage position after aligning to target position: -0.25 -0.39 (2nd move error -0.06 -0.23) Finished aligning to item in 1 rounds Trimming A by 22 120 100 20, reference by 22 120 95 20 -34.7 20.8 unbinned pixels; 121.6 93.6 nm along two shift axes; -73.4 -139.2 nm on specimen axes Total_Shift = 80.811903 Trimming A by 20 125 100 20, reference by 22 120 95 20 -4.7 12.1 unbinned pixels; 50.7 1.5 nm along two shift axes; -46.3 -20.7 nm on specimen axes Total_Shift = 26.021233 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.56 microns changed by -3.76 to target drift = 0.02 nm/sec Measured defocus = -1.20 microns changed by 0.00 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1400.tif 27 frames are being saved to U:\krios\20190717\PilA_1401.tif 27 frames are being saved to U:\krios\20190717\PilA_1402.tif 27 frames are being saved to U:\krios\20190717\PilA_1403.tif 27 frames are being saved to U:\krios\20190717\PilA_1404.tif 27 frames are being saved to U:\krios\20190717\PilA_1405.tif Running script 17 at item 303 (499.44, -383.95) Remaining time to fill dewars is 10980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 160 80 40, reference by 20 196 180 20 Disparity in stage position after aligning to target position: -0.19 -0.50 (2nd move error -0.00 -0.35) Finished aligning to item in 1 rounds Trimming A by 20 170 75 35, reference by 20 160 80 40 -22.0 0.1 unbinned pixels; 28.9 79.0 nm along two shift axes; 4.4 -85.5 nm on specimen axes Total_Shift = 43.926755 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.25 microns changed by -2.55 to target drift = 0.14 nm/sec Measured defocus = -1.35 microns changed by 0.05 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1406.tif 27 frames are being saved to U:\krios\20190717\PilA_1407.tif 27 frames are being saved to U:\krios\20190717\PilA_1408.tif 27 frames are being saved to U:\krios\20190717\PilA_1409.tif 27 frames are being saved to U:\krios\20190717\PilA_1410.tif 27 frames are being saved to U:\krios\20190717\PilA_1411.tif Running script 17 at item 304 (496.90, -382.37) Remaining time to fill dewars is 10800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 125 65 85, reference by 20 220 20 205 Disparity in stage position after aligning to target position: -0.05 -0.01 (2nd move error 0.10 0.21) Finished aligning to item in 1 rounds Trimming A by 22 115 20 85, reference by 21 125 65 85 -1.2 2.4 unbinned pixels; 10.5 0.7 nm along two shift axes; -9.2 -5.2 nm on specimen axes Total_Shift = 5.441852 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.22 microns changed by -3.62 to target drift = 0.13 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1412.tif 27 frames are being saved to U:\krios\20190717\PilA_1413.tif 27 frames are being saved to U:\krios\20190717\PilA_1414.tif 27 frames are being saved to U:\krios\20190717\PilA_1415.tif 27 frames are being saved to U:\krios\20190717\PilA_1416.tif 27 frames are being saved to U:\krios\20190717\PilA_1417.tif Running script 17 at item 305 (494.38, -381.03) Remaining time to fill dewars is 10560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 110 20 110, reference by 20 220 20 205 Disparity in stage position after aligning to target position: -0.11 -0.11 (2nd move error 0.04 0.11) Finished aligning to item in 1 rounds Trimming A by 21 110 55 100, reference by 24 110 20 110 -2.4 -0.6 unbinned pixels; 0.8 9.6 nm along two shift axes; 3.0 -9.2 nm on specimen axes Total_Shift = 4.946047 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.68 microns changed by -2.18 to target drift = 0.12 nm/sec Measured defocus = -1.48 microns changed by -0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1418.tif 27 frames are being saved to U:\krios\20190717\PilA_1419.tif 27 frames are being saved to U:\krios\20190717\PilA_1420.tif 27 frames are being saved to U:\krios\20190717\PilA_1421.tif 27 frames are being saved to U:\krios\20190717\PilA_1422.tif 27 frames are being saved to U:\krios\20190717\PilA_1423.tif Running script 17 at item 306 (491.83, -379.31) Remaining time to fill dewars is 10440 Dewars are NOT busy filling Trimming A by 23 225 20 200, reference by 162 22 20 200 Trimming A by 23 225 20 200, reference by 175 20 165 20 Trimming A by 23 225 20 200, reference by 20 220 20 205 Trimming A by 23 225 20 200, reference by 20 196 180 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.24 Trimming A by 21 90 20 125, reference by 20 220 20 205 Disparity in stage position after aligning to target position: -0.19 -0.23 (2nd move error -0.06 -0.01) Finished aligning to item in 2 rounds Trimming A by 21 90 20 125, reference by 21 90 20 125 -2.8 12.5 unbinned pixels; 49.7 8.6 nm along two shift axes; -48.1 -13.7 nm on specimen axes Total_Shift = 25.672115 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.91 microns changed by -3.51 to target drift = 0.08 nm/sec Measured defocus = -1.63 microns changed by 0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1424.tif 27 frames are being saved to U:\krios\20190717\PilA_1425.tif 27 frames are being saved to U:\krios\20190717\PilA_1426.tif 27 frames are being saved to U:\krios\20190717\PilA_1427.tif 27 frames are being saved to U:\krios\20190717\PilA_1428.tif 27 frames are being saved to U:\krios\20190717\PilA_1429.tif Running script 17 at item 307 (528.97, -461.38) Remaining time to fill dewars is 10140 Dewars are NOT busy filling Trimming A by 23 97 120 20, reference by 241 24 20 265 Trimming A by 23 97 120 20, reference by 210 23 140 20 Trimming A by 23 97 120 20, reference by 20 153 20 230 Trimming A by 23 97 120 20, reference by 21 100 100 20 Disparity in stage position after aligning to center of map frame: -0.32 -0.25 Trimming A by 90 56 175 20, reference by 21 100 100 20 Disparity in stage position after aligning to target position: -0.17 -0.05 (2nd move error 0.14 0.19) Finished aligning to item in 2 rounds Trimming A by 87 38 190 20, reference by 90 56 175 20 -0.7 -0.1 unbinned pixels; 0.5 2.7 nm along two shift axes; 0.6 -2.7 nm on specimen axes Total_Shift = 1.427838 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.07 microns changed by -2.77 to target drift = 0.15 nm/sec Measured defocus = -1.66 microns changed by -0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1430.tif 27 frames are being saved to U:\krios\20190717\PilA_1431.tif 27 frames are being saved to U:\krios\20190717\PilA_1432.tif 27 frames are being saved to U:\krios\20190717\PilA_1433.tif 27 frames are being saved to U:\krios\20190717\PilA_1434.tif 27 frames are being saved to U:\krios\20190717\PilA_1435.tif Running script 17 at item 308 (526.32, -459.71) Remaining time to fill dewars is 10020 Dewars are NOT busy filling Trimming A by 230 22 155 20, reference by 241 24 20 265 Trimming A by 230 22 155 20, reference by 210 23 140 20 Trimming A by 230 22 155 20, reference by 20 153 20 230 Trimming A by 230 22 155 20, reference by 21 100 100 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.30 Trimming A by 115 24 130 20, reference by 210 23 140 20 Disparity in stage position after aligning to target position: -0.05 -0.19 (2nd move error 0.24 0.09) Finished aligning to item in 2 rounds Trimming A by 115 20 140 20, reference by 115 24 130 20 3.9 11.4 unbinned pixels; 36.6 31.2 nm along two shift axes; -45.0 12.8 nm on specimen axes Total_Shift = 24.024872 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 2.08 microns changed by -3.88 to target drift = 0.18 nm/sec Measured defocus = -1.72 microns changed by -0.08 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1436.tif 27 frames are being saved to U:\krios\20190717\PilA_1437.tif 27 frames are being saved to U:\krios\20190717\PilA_1438.tif 27 frames are being saved to U:\krios\20190717\PilA_1439.tif 27 frames are being saved to U:\krios\20190717\PilA_1440.tif 27 frames are being saved to U:\krios\20190717\PilA_1441.tif Running script 17 at item 309 (523.88, -458.00) Remaining time to fill dewars is 9840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 20 110 20, reference by 210 23 140 20 Disparity in stage position after aligning to target position: -0.21 -0.32 (2nd move error 0.07 -0.04) Finished aligning to item in 1 rounds Trimming A by 130 20 115 20, reference by 130 20 110 20 -4.7 1.3 unbinned pixels; 11.0 14.7 nm along two shift axes; -4.3 -18.4 nm on specimen axes Total_Shift = 9.701675 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.60 microns changed by -2.50 to target drift = 0.08 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1442.tif 27 frames are being saved to U:\krios\20190717\PilA_1443.tif 27 frames are being saved to U:\krios\20190717\PilA_1444.tif 27 frames are being saved to U:\krios\20190717\PilA_1445.tif 27 frames are being saved to U:\krios\20190717\PilA_1446.tif 27 frames are being saved to U:\krios\20190717\PilA_1447.tif Running script 17 at item 310 (521.39, -456.27) Remaining time to fill dewars is 9660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 21 95 20, reference by 210 23 140 20 Disparity in stage position after aligning to target position: -0.22 -0.41 (2nd move error 0.06 -0.13) Finished aligning to item in 1 rounds Trimming A by 150 21 95 20, reference by 150 21 95 20 -15.7 -1.9 unbinned pixels; 13.6 59.4 nm along two shift axes; 10.6 -60.8 nm on specimen axes Total_Shift = 31.664442 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.67 microns changed by -3.67 to target drift = 0.09 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1448.tif 27 frames are being saved to U:\krios\20190717\PilA_1449.tif 27 frames are being saved to U:\krios\20190717\PilA_1450.tif 27 frames are being saved to U:\krios\20190717\PilA_1451.tif 27 frames are being saved to U:\krios\20190717\PilA_1452.tif 27 frames are being saved to U:\krios\20190717\PilA_1453.tif Running script 17 at item 311 (518.83, -454.56) Remaining time to fill dewars is 9480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 180 20 65 95, reference by 210 23 140 20 Disparity in stage position after aligning to target position: -0.32 -0.53 (2nd move error -0.03 -0.24) Finished aligning to item in 1 rounds Trimming A by 180 20 60 95, reference by 180 20 65 95 -34.9 12.2 unbinned pixels; 90.5 107.3 nm along two shift axes; -40.1 -138.4 nm on specimen axes Total_Shift = 73.977511 Trimming A by 180 20 50 90, reference by 180 20 65 95 0.9 5.1 unbinned pixels; 17.7 11.0 nm along two shift axes; -20.1 2.5 nm on specimen axes Total_Shift = 10.411708 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.12 microns changed by -2.22 to target drift = 0.03 nm/sec Measured defocus = -2.12 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1454.tif 27 frames are being saved to U:\krios\20190717\PilA_1455.tif 27 frames are being saved to U:\krios\20190717\PilA_1456.tif 27 frames are being saved to U:\krios\20190717\PilA_1457.tif 27 frames are being saved to U:\krios\20190717\PilA_1458.tif 27 frames are being saved to U:\krios\20190717\PilA_1459.tif Running script 17 at item 312 (517.30, -457.77) Remaining time to fill dewars is 9300 Dewars are NOT busy filling Trimming A by 250 22 20 270, reference by 241 24 20 265 Trimming A by 250 22 20 270, reference by 210 23 140 20 Trimming A by 250 22 20 270, reference by 20 153 20 230 Trimming A by 250 22 20 270, reference by 21 100 100 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.20 Trimming A by 125 23 20 105, reference by 241 24 20 265 Disparity in stage position after aligning to target position: -0.03 0.13 (2nd move error 0.16 0.31) Finished aligning to item in 2 rounds Trimming A by 130 22 20 105, reference by 125 23 20 105 0.7 -2.4 unbinned pixels; 9.7 1.2 nm along two shift axes; 9.2 3.1 nm on specimen axes Total_Shift = 4.980015 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.31 microns changed by -3.51 to target drift = 0.15 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1460.tif 27 frames are being saved to U:\krios\20190717\PilA_1461.tif 27 frames are being saved to U:\krios\20190717\PilA_1462.tif 27 frames are being saved to U:\krios\20190717\PilA_1463.tif 27 frames are being saved to U:\krios\20190717\PilA_1464.tif 27 frames are being saved to U:\krios\20190717\PilA_1465.tif Running script 17 at item 313 (519.75, -458.83) Remaining time to fill dewars is 9120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 20 35 50, reference by 210 23 140 20 Disparity in stage position after aligning to target position: -0.15 -0.41 (2nd move error 0.13 -0.13) Finished aligning to item in 1 rounds Trimming A by 70 20 35 45, reference by 70 20 35 50 -15.0 -1.8 unbinned pixels; 12.8 56.7 nm along two shift axes; 10.3 -57.9 nm on specimen axes Total_Shift = 30.204053 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.04 microns changed by -2.26 to target drift = 0.14 nm/sec Measured defocus = -2.27 microns changed by -0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1466.tif 27 frames are being saved to U:\krios\20190717\PilA_1467.tif 27 frames are being saved to U:\krios\20190717\PilA_1468.tif 27 frames are being saved to U:\krios\20190717\PilA_1469.tif 27 frames are being saved to U:\krios\20190717\PilA_1470.tif 27 frames are being saved to U:\krios\20190717\PilA_1471.tif Running script 17 at item 314 (522.21, -460.55) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Trimming A by 224 24 160 20, reference by 241 24 20 265 Trimming A by 224 24 160 20, reference by 210 23 140 20 Trimming A by 224 24 160 20, reference by 20 153 20 230 Trimming A by 224 24 160 20, reference by 21 100 100 20 Disparity in stage position after aligning to center of map frame: -0.24 -0.23 Trimming A by 60 24 50 20, reference by 210 23 140 20 Disparity in stage position after aligning to target position: -0.15 -0.41 (2nd move error 0.08 -0.20) Finished aligning to item in 2 rounds Trimming A by 55 20 50 20, reference by 60 24 50 20 -24.5 -6.9 unbinned pixels; 6.7 98.5 nm along two shift axes; 31.9 -93.8 nm on specimen axes Total_Shift = 50.87239 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.34 microns changed by -3.74 to target drift = 0.15 nm/sec Measured defocus = -2.43 microns changed by 0.03 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1472.tif 27 frames are being saved to U:\krios\20190717\PilA_1473.tif 27 frames are being saved to U:\krios\20190717\PilA_1474.tif 27 frames are being saved to U:\krios\20190717\PilA_1475.tif 27 frames are being saved to U:\krios\20190717\PilA_1476.tif 27 frames are being saved to U:\krios\20190717\PilA_1477.tif Running script 17 at item 315 (524.84, -462.24) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 21 105 110 20, reference by 241 24 20 265 Trimming A by 21 105 110 20, reference by 210 23 140 20 Trimming A by 21 105 110 20, reference by 20 153 20 230 Trimming A by 21 105 110 20, reference by 21 100 100 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.26 Trimming A by 50 45 65 20, reference by 21 100 100 20 Disparity in stage position after aligning to target position: -0.30 -0.21 (2nd move error -0.06 0.02) Finished aligning to item in 2 rounds Trimming A by 50 40 65 20, reference by 50 45 65 20 0.8 13.9 unbinned pixels; 50.2 23.7 nm along two shift axes; -54.3 0.0 nm on specimen axes Total_Shift = 27.861044 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.07 microns changed by -2.57 to target drift = 0.16 nm/sec Measured defocus = -2.52 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1478.tif 27 frames are being saved to U:\krios\20190717\PilA_1479.tif 27 frames are being saved to U:\krios\20190717\PilA_1480.tif 27 frames are being saved to U:\krios\20190717\PilA_1481.tif 27 frames are being saved to U:\krios\20190717\PilA_1482.tif 27 frames are being saved to U:\krios\20190717\PilA_1483.tif Running script 17 at item 316 (527.34, -463.96) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 69 165 20, reference by 21 100 100 20 Disparity in stage position after aligning to target position: -0.38 -0.11 (2nd move error -0.14 0.13) Finished aligning to item in 1 rounds Trimming A by 20 68 160 20, reference by 23 69 165 20 4.4 26.7 unbinned pixels; 92.4 55.8 nm along two shift axes; -104.7 11.3 nm on specimen axes Total_Shift = 54.061665 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.09 microns changed by -2.69 to target drift = 0.16 nm/sec Measured defocus = -2.63 microns changed by 0.03 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1484.tif 27 frames are being saved to U:\krios\20190717\PilA_1485.tif 27 frames are being saved to U:\krios\20190717\PilA_1486.tif 27 frames are being saved to U:\krios\20190717\PilA_1487.tif 27 frames are being saved to U:\krios\20190717\PilA_1488.tif 27 frames are being saved to U:\krios\20190717\PilA_1489.tif Running script 17 at item 317 (525.67, -466.59) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 73 135 20, reference by 21 100 100 20 Disparity in stage position after aligning to target position: -0.07 -0.23 (2nd move error 0.17 0.00) Finished aligning to item in 1 rounds Trimming A by 23 73 140 20, reference by 23 73 135 20 1.2 -6.1 unbinned pixels; 24.1 4.7 nm along two shift axes; 23.5 6.1 nm on specimen axes Total_Shift = 12.452992 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.50 microns changed by -2.60 to target drift = 0.13 nm/sec Measured defocus = -1.04 microns changed by -0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1490.tif 27 frames are being saved to U:\krios\20190717\PilA_1491.tif 27 frames are being saved to U:\krios\20190717\PilA_1492.tif 27 frames are being saved to U:\krios\20190717\PilA_1493.tif 27 frames are being saved to U:\krios\20190717\PilA_1494.tif 27 frames are being saved to U:\krios\20190717\PilA_1495.tif Running script 17 at item 318 (523.16, -464.81) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 35 50 20, reference by 21 100 100 20 Disparity in stage position after aligning to target position: -0.12 -0.37 (2nd move error 0.12 -0.14) Finished aligning to item in 1 rounds Trimming A by 22 35 55 20, reference by 22 35 50 20 -15.2 -4.9 unbinned pixels; 1.8 62.1 nm along two shift axes; 22.3 -58.1 nm on specimen axes Total_Shift = 31.9223 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.77 microns changed by -3.97 to target drift = 0.10 nm/sec Measured defocus = -1.25 microns changed by 0.05 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1496.tif 27 frames are being saved to U:\krios\20190717\PilA_1497.tif 27 frames are being saved to U:\krios\20190717\PilA_1498.tif 27 frames are being saved to U:\krios\20190717\PilA_1499.tif 27 frames are being saved to U:\krios\20190717\PilA_1500.tif 27 frames are being saved to U:\krios\20190717\PilA_1501.tif Running script 17 at item 319 (520.67, -463.01) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 21 100 100 20 Disparity in stage position after aligning to target position: -0.22 -0.46 (2nd move error 0.03 -0.23) Finished aligning to item in 1 rounds -29.8 -3.0 unbinned pixels; 28.0 111.9 nm along two shift axes; 17.8 -115.5 nm on specimen axes Total_Shift = 59.98214 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.20 microns changed by -2.50 to target drift = 0.14 nm/sec Measured defocus = -1.36 microns changed by 0.06 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1502.tif 27 frames are being saved to U:\krios\20190717\PilA_1503.tif 27 frames are being saved to U:\krios\20190717\PilA_1504.tif 27 frames are being saved to U:\krios\20190717\PilA_1505.tif 27 frames are being saved to U:\krios\20190717\PilA_1506.tif 27 frames are being saved to U:\krios\20190717\PilA_1507.tif Running script 17 at item 320 (518.00, -461.32) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Trimming A by 225 20 155 20, reference by 241 24 20 265 Trimming A by 225 20 155 20, reference by 210 23 140 20 Trimming A by 225 20 155 20, reference by 20 153 20 230 Trimming A by 225 20 155 20, reference by 21 100 100 20 Disparity in stage position after aligning to center of map frame: -0.19 -0.27 Trimming A by 50 21 20 55, reference by 210 23 140 20 Disparity in stage position after aligning to target position: -0.10 -0.63 (2nd move error 0.09 -0.38) Finished aligning to item in 2 rounds Trimming A by 45 21 20 50, reference by 50 21 20 55 -18.8 -6.3 unbinned pixels; 1.4 77.3 nm along two shift axes; 28.5 -71.9 nm on specimen axes Total_Shift = 39.715139 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.28 microns changed by -3.68 to target drift = 0.18 nm/sec Measured defocus = -1.48 microns changed by 0.08 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1508.tif 27 frames are being saved to U:\krios\20190717\PilA_1509.tif 27 frames are being saved to U:\krios\20190717\PilA_1510.tif 27 frames are being saved to U:\krios\20190717\PilA_1511.tif 27 frames are being saved to U:\krios\20190717\PilA_1512.tif 27 frames are being saved to U:\krios\20190717\PilA_1513.tif Running script 17 at item 321 (515.45, -460.28) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Trimming A by 250 22 20 270, reference by 241 24 20 265 Trimming A by 250 22 20 270, reference by 210 23 140 20 Trimming A by 250 22 20 270, reference by 20 153 20 230 Trimming A by 250 22 20 270, reference by 21 100 100 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.22 Trimming A by 92 23 20 135, reference by 241 24 20 265 Disparity in stage position after aligning to target position: -0.02 0.01 (2nd move error 0.17 0.20) Finished aligning to item in 2 rounds Trimming A by 105 20 20 120, reference by 92 23 20 135 2.5 0.5 unbinned pixels; 1.4 9.8 nm along two shift axes; -2.5 9.6 nm on specimen axes Total_Shift = 5.099845 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.71 microns changed by -2.21 to target drift = 0.13 nm/sec Measured defocus = -1.50 microns changed by -0.00 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1514.tif 27 frames are being saved to U:\krios\20190717\PilA_1515.tif 27 frames are being saved to U:\krios\20190717\PilA_1516.tif 27 frames are being saved to U:\krios\20190717\PilA_1517.tif 27 frames are being saved to U:\krios\20190717\PilA_1518.tif 27 frames are being saved to U:\krios\20190717\PilA_1519.tif Running script 17 at item 322 (514.12, -462.86) Remaining time to fill dewars is 8880 Dewars are NOT busy filling Trimming A by 22 156 20 225, reference by 241 24 20 265 Trimming A by 22 156 20 225, reference by 210 23 140 20 Trimming A by 22 156 20 225, reference by 20 153 20 230 Trimming A by 22 156 20 225, reference by 21 100 100 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.25 Trimming A by 61 23 20 145, reference by 20 153 20 230 Disparity in stage position after aligning to target position: -0.20 -0.13 (2nd move error -0.01 0.09) Finished aligning to item in 2 rounds Trimming A by 58 23 20 150, reference by 61 23 20 145 1.2 2.5 unbinned pixels; 7.7 8.1 nm along two shift axes; -10.0 4.1 nm on specimen axes Total_Shift = 5.573099 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.91 microns changed by -3.51 to target drift = 0.14 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1520.tif 27 frames are being saved to U:\krios\20190717\PilA_1521.tif 27 frames are being saved to U:\krios\20190717\PilA_1522.tif 27 frames are being saved to U:\krios\20190717\PilA_1523.tif 27 frames are being saved to U:\krios\20190717\PilA_1524.tif 27 frames are being saved to U:\krios\20190717\PilA_1525.tif Running script 17 at item 323 (516.54, -464.48) Remaining time to fill dewars is 7680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 50 20 70, reference by 20 153 20 230 Disparity in stage position after aligning to target position: -0.23 -0.02 (2nd move error -0.04 0.20) Finished aligning to item in 1 rounds Trimming A by 21 45 20 70, reference by 21 50 20 70 3.6 8.0 unbinned pixels; 24.7 25.1 nm along two shift axes; -31.9 12.4 nm on specimen axes Total_Shift = 17.546564 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.52 microns changed by -2.22 to target drift = 0.17 nm/sec Measured defocus = -1.62 microns changed by -0.08 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1526.tif 27 frames are being saved to U:\krios\20190717\PilA_1527.tif 27 frames are being saved to U:\krios\20190717\PilA_1528.tif 27 frames are being saved to U:\krios\20190717\PilA_1529.tif 27 frames are being saved to U:\krios\20190717\PilA_1530.tif 27 frames are being saved to U:\krios\20190717\PilA_1531.tif Running script 17 at item 324 (519.04, -465.64) Remaining time to fill dewars is 7500 Dewars are NOT busy filling Trimming A by 20 101 115 20, reference by 241 24 20 265 Trimming A by 20 101 115 20, reference by 210 23 140 20 Trimming A by 20 101 115 20, reference by 20 153 20 230 Trimming A by 20 101 115 20, reference by 21 100 100 20 Disparity in stage position after aligning to center of map frame: -0.22 -0.21 Trimming A by 21 45 20 55, reference by 21 100 100 20 Disparity in stage position after aligning to target position: -0.25 -0.58 (2nd move error -0.04 -0.39) Finished aligning to item in 2 rounds Trimming A by 21 50 20 60, reference by 21 45 20 55 -28.6 33.8 unbinned pixels; 161.7 51.9 nm along two shift axes; -125.6 -118.3 nm on specimen axes Total_Shift = 88.55752 Trimming A by 20 55 20 55, reference by 21 45 20 55 0.1 22.5 unbinned pixels; 82.6 34.2 nm along two shift axes; -87.6 -4.4 nm on specimen axes Total_Shift = 45.004786 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 2.02 microns changed by -3.82 to target drift = 0.05 nm/sec Measured defocus = -1.89 microns changed by 0.09 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1532.tif 27 frames are being saved to U:\krios\20190717\PilA_1533.tif 27 frames are being saved to U:\krios\20190717\PilA_1534.tif 27 frames are being saved to U:\krios\20190717\PilA_1535.tif 27 frames are being saved to U:\krios\20190717\PilA_1536.tif 27 frames are being saved to U:\krios\20190717\PilA_1537.tif Running script 17 at item 325 (521.51, -467.39) Remaining time to fill dewars is 7320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 65 40 45, reference by 21 100 100 20 Disparity in stage position after aligning to target position: -0.19 -0.44 (2nd move error 0.02 -0.25) Finished aligning to item in 1 rounds Trimming A by 21 65 40 55, reference by 21 65 40 45 -30.5 -1.5 unbinned pixels; 34.2 112.2 nm along two shift axes; 12.4 -118.4 nm on specimen axes Total_Shift = 61.108698 Trimming A by 21 70 35 55, reference by 21 65 40 45 -1.2 -3.8 unbinned pixels; 12.5 9.9 nm along two shift axes; 15.1 -3.7 nm on specimen axes Total_Shift = 7.982043 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.70 microns changed by -2.60 to target drift = 0.14 nm/sec Measured defocus = -1.95 microns changed by 0.05 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1538.tif 27 frames are being saved to U:\krios\20190717\PilA_1539.tif 27 frames are being saved to U:\krios\20190717\PilA_1540.tif 27 frames are being saved to U:\krios\20190717\PilA_1541.tif 27 frames are being saved to U:\krios\20190717\PilA_1542.tif 27 frames are being saved to U:\krios\20190717\PilA_1543.tif Running script 17 at item 326 (523.99, -469.14) Remaining time to fill dewars is 7140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 130 90 20, reference by 21 100 100 20 Disparity in stage position after aligning to target position: -0.14 -0.29 (2nd move error 0.07 -0.10) Finished aligning to item in 1 rounds Trimming A by 22 130 95 20, reference by 21 130 90 20 -10.8 1.4 unbinned pixels; 19.3 36.9 nm along two shift axes; -3.2 -42.5 nm on specimen axes Total_Shift = 21.86013 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 2.01 microns changed by -4.01 to target drift = 0.10 nm/sec Measured defocus = -2.04 microns changed by 0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1544.tif 27 frames are being saved to U:\krios\20190717\PilA_1545.tif 27 frames are being saved to U:\krios\20190717\PilA_1546.tif 27 frames are being saved to U:\krios\20190717\PilA_1547.tif 27 frames are being saved to U:\krios\20190717\PilA_1548.tif 27 frames are being saved to U:\krios\20190717\PilA_1549.tif Running script 17 at item 327 (499.30, -515.25) Remaining time to fill dewars is 6960 Dewars are NOT busy filling Trimming A by 200 20 155 20, reference by 200 24 20 220 Trimming A by 200 20 155 20, reference by 180 23 145 20 Trimming A by 200 20 155 20, reference by 20 145 20 170 Trimming A by 200 20 155 20, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.28 Trimming A by 130 20 170 20, reference by 180 23 145 20 Disparity in stage position after aligning to target position: -0.14 -0.02 (2nd move error 0.15 0.24) Finished aligning to item in 2 rounds Trimming A by 135 21 180 20, reference by 130 20 170 20 -2.8 -3.2 unbinned pixels; 8.0 15.0 nm along two shift axes; 13.0 -10.4 nm on specimen axes Total_Shift = 8.544357 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.73 microns changed by -2.83 to target drift = 0.13 nm/sec Measured defocus = -2.09 microns changed by -0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1550.tif 27 frames are being saved to U:\krios\20190717\PilA_1551.tif 27 frames are being saved to U:\krios\20190717\PilA_1552.tif 27 frames are being saved to U:\krios\20190717\PilA_1553.tif 27 frames are being saved to U:\krios\20190717\PilA_1554.tif 27 frames are being saved to U:\krios\20190717\PilA_1555.tif Running script 17 at item 328 (496.91, -513.57) Remaining time to fill dewars is 6780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 22 140 20, reference by 180 23 145 20 Disparity in stage position after aligning to target position: -0.10 -0.20 (2nd move error 0.17 0.05) Finished aligning to item in 1 rounds Trimming A by 145 22 140 20, reference by 140 22 140 20 2.0 5.2 unbinned pixels; 16.3 14.9 nm along two shift axes; -20.5 6.7 nm on specimen axes Total_Shift = 11.053877 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.45 microns changed by -2.65 to target drift = 0.10 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1556.tif 27 frames are being saved to U:\krios\20190717\PilA_1557.tif 27 frames are being saved to U:\krios\20190717\PilA_1558.tif 27 frames are being saved to U:\krios\20190717\PilA_1559.tif 27 frames are being saved to U:\krios\20190717\PilA_1560.tif 27 frames are being saved to U:\krios\20190717\PilA_1561.tif Running script 17 at item 329 (494.41, -511.91) Remaining time to fill dewars is 6600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 160 22 130 20, reference by 180 23 145 20 Disparity in stage position after aligning to target position: -0.18 -0.31 (2nd move error 0.09 -0.06) Finished aligning to item in 1 rounds Trimming A by 160 22 130 20, reference by 160 22 130 20 -6.2 0.3 unbinned pixels; 9.0 21.8 nm along two shift axes; 0.3 -24.0 nm on specimen axes Total_Shift = 12.32021 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.48 microns changed by -3.78 to target drift = 0.09 nm/sec Measured defocus = -2.47 microns changed by 0.17 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1562.tif 27 frames are being saved to U:\krios\20190717\PilA_1563.tif 27 frames are being saved to U:\krios\20190717\PilA_1564.tif 27 frames are being saved to U:\krios\20190717\PilA_1565.tif 27 frames are being saved to U:\krios\20190717\PilA_1566.tif 27 frames are being saved to U:\krios\20190717\PilA_1567.tif Running script 17 at item 330 (489.39, -508.37) Remaining time to fill dewars is 6420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 200 20 105 40, reference by 180 23 145 20 Disparity in stage position after aligning to target position: -0.26 -0.47 (2nd move error 0.02 -0.21) Finished aligning to item in 1 rounds Trimming A by 200 20 95 35, reference by 200 20 105 40 -23.6 -2.3 unbinned pixels; 22.4 88.6 nm along two shift axes; 14.0 -91.5 nm on specimen axes Total_Shift = 47.513206 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.16 microns changed by -2.24 to target drift = 0.11 nm/sec Measured defocus = -2.45 microns changed by 0.05 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1568.tif 27 frames are being saved to U:\krios\20190717\PilA_1569.tif 27 frames are being saved to U:\krios\20190717\PilA_1570.tif 27 frames are being saved to U:\krios\20190717\PilA_1571.tif 27 frames are being saved to U:\krios\20190717\PilA_1572.tif 27 frames are being saved to U:\krios\20190717\PilA_1573.tif Running script 17 at item 331 (487.69, -511.48) Remaining time to fill dewars is 6240 Dewars are NOT busy filling Trimming A by 215 23 20 220, reference by 200 24 20 220 Trimming A by 215 23 20 220, reference by 180 23 145 20 Trimming A by 215 23 20 220, reference by 20 145 20 170 Trimming A by 215 23 20 220, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.19 -0.21 Trimming A by 120 20 20 80, reference by 200 24 20 220 Disparity in stage position after aligning to target position: -0.04 0.07 (2nd move error 0.14 0.25) Finished aligning to item in 2 rounds Trimming A by 125 22 25 80, reference by 120 20 20 80 3.6 -0.8 unbinned pixels; 7.4 11.7 nm along two shift axes; 2.2 14.0 nm on specimen axes Total_Shift = 7.284967 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.88 microns changed by -3.38 to target drift = 0.12 nm/sec Measured defocus = -2.47 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1574.tif 27 frames are being saved to U:\krios\20190717\PilA_1575.tif 27 frames are being saved to U:\krios\20190717\PilA_1576.tif 27 frames are being saved to U:\krios\20190717\PilA_1577.tif 27 frames are being saved to U:\krios\20190717\PilA_1578.tif 27 frames are being saved to U:\krios\20190717\PilA_1579.tif Running script 17 at item 332 (490.30, -512.71) Remaining time to fill dewars is 6060 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 90 24 70 20, reference by 180 23 145 20 Disparity in stage position after aligning to target position: -0.17 -0.45 (2nd move error 0.12 -0.20) Finished aligning to item in 1 rounds Trimming A by 90 24 70 20, reference by 90 24 70 20 -17.6 -0.9 unbinned pixels; 19.6 64.8 nm along two shift axes; 7.3 -68.3 nm on specimen axes Total_Shift = 35.283516 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.39 microns changed by -2.21 to target drift = 0.15 nm/sec Measured defocus = -2.64 microns changed by 0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1580.tif 27 frames are being saved to U:\krios\20190717\PilA_1581.tif 27 frames are being saved to U:\krios\20190717\PilA_1582.tif 27 frames are being saved to U:\krios\20190717\PilA_1583.tif 27 frames are being saved to U:\krios\20190717\PilA_1584.tif 27 frames are being saved to U:\krios\20190717\PilA_1585.tif Running script 17 at item 333 (492.75, -514.41) Remaining time to fill dewars is 5820 Dewars are NOT busy filling Trimming A by 200 20 150 20, reference by 200 24 20 220 Trimming A by 200 20 150 20, reference by 180 23 145 20 Trimming A by 200 20 150 20, reference by 20 145 20 170 Trimming A by 200 20 150 20, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.21 -0.23 Trimming A by 80 20 75 20, reference by 180 23 145 20 Disparity in stage position after aligning to target position: -0.10 -0.33 (2nd move error 0.10 -0.13) Finished aligning to item in 2 rounds Trimming A by 80 20 75 20, reference by 80 20 75 20 -14.9 -3.3 unbinned pixels; 7.3 58.5 nm along two shift axes; 16.0 -57.2 nm on specimen axes Total_Shift = 30.467219 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.74 microns changed by -3.84 to target drift = 0.10 nm/sec Measured defocus = -1.15 microns changed by 0.05 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1586.tif 27 frames are being saved to U:\krios\20190717\PilA_1587.tif 27 frames are being saved to U:\krios\20190717\PilA_1588.tif 27 frames are being saved to U:\krios\20190717\PilA_1589.tif 27 frames are being saved to U:\krios\20190717\PilA_1590.tif 27 frames are being saved to U:\krios\20190717\PilA_1591.tif Running script 17 at item 334 (497.87, -517.87) Remaining time to fill dewars is 5700 Dewars are NOT busy filling Trimming A by 20 80 135 20, reference by 200 24 20 220 Trimming A by 20 80 135 20, reference by 180 23 145 20 Trimming A by 20 80 135 20, reference by 20 145 20 170 Trimming A by 20 80 135 20, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.21 -0.32 Trimming A by 33 43 135 20, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.24 -0.13 (2nd move error -0.04 0.17) Finished aligning to item in 2 rounds Trimming A by 51 51 125 20, reference by 33 43 135 20 0.3 9.8 unbinned pixels; 35.8 15.8 nm along two shift axes; -38.3 -1.0 nm on specimen axes Total_Shift = 19.678662 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 3.03 microns changed by -4.23 to target drift = 0.17 nm/sec Measured defocus = -1.27 microns changed by 0.07 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1592.tif 27 frames are being saved to U:\krios\20190717\PilA_1593.tif 27 frames are being saved to U:\krios\20190717\PilA_1594.tif 27 frames are being saved to U:\krios\20190717\PilA_1595.tif 27 frames are being saved to U:\krios\20190717\PilA_1596.tif 27 frames are being saved to U:\krios\20190717\PilA_1597.tif Running script 17 at item 335 (498.73, -522.09) Remaining time to fill dewars is 5460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 115 200 20, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.18 -0.11 (2nd move error 0.02 0.18) Finished aligning to item in 1 rounds Trimming A by 21 119 195 20, reference by 22 115 200 20 -1.0 -0.2 unbinned pixels; 0.5 4.0 nm along two shift axes; 1.0 -3.9 nm on specimen axes Total_Shift = 2.071432 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.57 microns changed by -2.87 to target drift = 0.13 nm/sec Measured defocus = -1.27 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1598.tif 27 frames are being saved to U:\krios\20190717\PilA_1599.tif 27 frames are being saved to U:\krios\20190717\PilA_1600.tif 27 frames are being saved to U:\krios\20190717\PilA_1601.tif 27 frames are being saved to U:\krios\20190717\PilA_1602.tif 27 frames are being saved to U:\krios\20190717\PilA_1603.tif Running script 17 at item 336 (496.23, -520.37) Remaining time to fill dewars is 5280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 46 125 20, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.12 -0.32 (2nd move error 0.08 -0.02) Finished aligning to item in 1 rounds Trimming A by 22 50 125 20, reference by 24 46 125 20 -3.2 -4.0 unbinned pixels; 10.6 17.5 nm along two shift axes; 16.3 -11.5 nm on specimen axes Total_Shift = 10.245569 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.79 microns changed by -4.19 to target drift = 0.11 nm/sec Measured defocus = -1.49 microns changed by 0.09 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1604.tif 27 frames are being saved to U:\krios\20190717\PilA_1605.tif 27 frames are being saved to U:\krios\20190717\PilA_1606.tif 27 frames are being saved to U:\krios\20190717\PilA_1607.tif 27 frames are being saved to U:\krios\20190717\PilA_1608.tif 27 frames are being saved to U:\krios\20190717\PilA_1609.tif Running script 17 at item 337 (493.74, -518.60) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 25 25 20, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.20 -0.40 (2nd move error -0.00 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 20 30 20, reference by 21 25 25 20 -14.2 2.9 unbinned pixels; 29.2 46.7 nm along two shift axes; -8.3 -55.8 nm on specimen axes Total_Shift = 28.953107 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.06 microns changed by -2.56 to target drift = 0.05 nm/sec Measured defocus = -1.48 microns changed by -0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1610.tif 27 frames are being saved to U:\krios\20190717\PilA_1611.tif 27 frames are being saved to U:\krios\20190717\PilA_1612.tif 27 frames are being saved to U:\krios\20190717\PilA_1613.tif 27 frames are being saved to U:\krios\20190717\PilA_1614.tif 27 frames are being saved to U:\krios\20190717\PilA_1615.tif Running script 17 at item 338 (491.07, -516.88) Remaining time to fill dewars is 4920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 180 23 145 20 Disparity in stage position after aligning to target position: -0.16 -0.44 (2nd move error 0.05 -0.24) Finished aligning to item in 1 rounds -26.8 -6.2 unbinned pixels; 12.0 105.9 nm along two shift axes; 29.9 -102.9 nm on specimen axes Total_Shift = 55.036854 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.23 microns changed by -3.83 to target drift = 0.19 nm/sec Measured defocus = -1.71 microns changed by 0.11 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1616.tif 27 frames are being saved to U:\krios\20190717\PilA_1617.tif 27 frames are being saved to U:\krios\20190717\PilA_1618.tif 27 frames are being saved to U:\krios\20190717\PilA_1619.tif 27 frames are being saved to U:\krios\20190717\PilA_1620.tif 27 frames are being saved to U:\krios\20190717\PilA_1621.tif Running script 17 at item 339 (488.64, -515.17) Remaining time to fill dewars is 4680 Dewars are NOT busy filling Trimming A by 200 20 150 20, reference by 200 24 20 220 Trimming A by 200 20 150 20, reference by 180 23 145 20 Trimming A by 200 20 150 20, reference by 20 145 20 170 Trimming A by 200 20 150 20, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.16 -0.26 Trimming A by 40 20 20 30, reference by 180 23 145 20 Disparity in stage position after aligning to target position: -0.15 -0.52 (2nd move error 0.01 -0.28) Finished aligning to item in 2 rounds Trimming A by 40 20 20 20, reference by 40 20 20 30 -24.0 0.2 unbinned pixels; 32.0 86.1 nm along two shift axes; 4.3 -93.3 nm on specimen axes Total_Shift = 47.968438 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.69 microns changed by -2.39 to target drift = 0.13 nm/sec Measured defocus = -1.77 microns changed by 0.07 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1622.tif 27 frames are being saved to U:\krios\20190717\PilA_1623.tif 27 frames are being saved to U:\krios\20190717\PilA_1624.tif 27 frames are being saved to U:\krios\20190717\PilA_1625.tif 27 frames are being saved to U:\krios\20190717\PilA_1626.tif 27 frames are being saved to U:\krios\20190717\PilA_1627.tif Running script 17 at item 340 (486.08, -514.08) Remaining time to fill dewars is 4560 Dewars are NOT busy filling Trimming A by 215 23 20 220, reference by 200 24 20 220 Trimming A by 215 23 20 220, reference by 180 23 145 20 Trimming A by 215 23 20 220, reference by 20 145 20 170 Trimming A by 215 23 20 220, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.17 -0.22 Trimming A by 100 21 60 85, reference by 200 24 20 220 Disparity in stage position after aligning to target position: 0.05 -0.06 (2nd move error 0.21 0.14) Finished aligning to item in 2 rounds Trimming A by 100 21 60 85, reference by 100 21 60 85 0.1 -3.7 unbinned pixels; 13.7 5.3 nm along two shift axes; 14.4 1.0 nm on specimen axes Total_Shift = 7.409622 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.70 microns changed by -3.50 to target drift = 0.14 nm/sec Measured defocus = -1.84 microns changed by 0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1628.tif 27 frames are being saved to U:\krios\20190717\PilA_1629.tif 27 frames are being saved to U:\krios\20190717\PilA_1630.tif 27 frames are being saved to U:\krios\20190717\PilA_1631.tif 27 frames are being saved to U:\krios\20190717\PilA_1632.tif 27 frames are being saved to U:\krios\20190717\PilA_1633.tif Running script 17 at item 341 (481.93, -514.92) Remaining time to fill dewars is 4380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 113 21 20 205, reference by 200 24 20 220 Disparity in stage position after aligning to target position: 0.00 -0.29 (2nd move error 0.17 -0.09) Finished aligning to item in 1 rounds Trimming A by 107 20 20 200, reference by 113 21 20 205 -7.9 -1.5 unbinned pixels; 4.8 30.5 nm along two shift axes; 7.5 -30.3 nm on specimen axes Total_Shift = 16.00105 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.06 microns changed by -1.96 to target drift = 0.13 nm/sec Measured defocus = -1.91 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1634.tif 27 frames are being saved to U:\krios\20190717\PilA_1635.tif 27 frames are being saved to U:\krios\20190717\PilA_1636.tif 27 frames are being saved to U:\krios\20190717\PilA_1637.tif 27 frames are being saved to U:\krios\20190717\PilA_1638.tif 27 frames are being saved to U:\krios\20190717\PilA_1639.tif Running script 17 at item 342 (483.63, -512.30) Remaining time to fill dewars is 4200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 165 22 20 160, reference by 200 24 20 220 Disparity in stage position after aligning to target position: -0.03 -0.21 (2nd move error 0.14 -0.01) Finished aligning to item in 1 rounds Trimming A by 165 21 20 160, reference by 165 22 20 160 -2.3 -5.4 unbinned pixels; 16.7 16.2 nm along two shift axes; 21.3 -7.7 nm on specimen axes Total_Shift = 11.630899 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.26 microns changed by -3.26 to target drift = 0.13 nm/sec Measured defocus = -2.03 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1640.tif 27 frames are being saved to U:\krios\20190717\PilA_1641.tif 27 frames are being saved to U:\krios\20190717\PilA_1642.tif 27 frames are being saved to U:\krios\20190717\PilA_1643.tif 27 frames are being saved to U:\krios\20190717\PilA_1644.tif 27 frames are being saved to U:\krios\20190717\PilA_1645.tif Running script 17 at item 343 (484.42, -516.65) Remaining time to fill dewars is 4020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 51 21 20 130, reference by 200 24 20 220 Disparity in stage position after aligning to target position: 0.14 -0.22 (2nd move error 0.31 -0.02) Finished aligning to item in 1 rounds Trimming A by 53 23 20 120, reference by 51 21 20 130 0.3 -3.3 unbinned pixels; 12.5 4.0 nm along two shift axes; 12.8 1.7 nm on specimen axes Total_Shift = 6.605879 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.02 microns changed by -2.12 to target drift = 0.14 nm/sec Measured defocus = -2.12 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1646.tif 27 frames are being saved to U:\krios\20190717\PilA_1647.tif 27 frames are being saved to U:\krios\20190717\PilA_1648.tif 27 frames are being saved to U:\krios\20190717\PilA_1649.tif 27 frames are being saved to U:\krios\20190717\PilA_1650.tif 27 frames are being saved to U:\krios\20190717\PilA_1651.tif Running script 17 at item 344 (487.11, -518.35) Remaining time to fill dewars is 3840 Dewars are NOT busy filling Trimming A by 24 150 20 170, reference by 200 24 20 220 Trimming A by 24 150 20 170, reference by 180 23 145 20 Trimming A by 24 150 20 170, reference by 20 145 20 170 Trimming A by 24 150 20 170, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.28 Trimming A by 26 20 20 20, reference by 20 145 20 170 Disparity in stage position after aligning to target position: -0.13 0.03 (2nd move error 0.00 0.28) Finished aligning to item in 2 rounds Trimming A by 21 20 20 30, reference by 26 20 20 20 -2.4 2.2 unbinned pixels; 11.1 5.4 nm along two shift axes; -7.9 -9.8 nm on specimen axes Total_Shift = 6.468955 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.40 microns changed by -3.60 to target drift = 0.08 nm/sec Measured defocus = -2.21 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1652.tif 27 frames are being saved to U:\krios\20190717\PilA_1653.tif 27 frames are being saved to U:\krios\20190717\PilA_1654.tif 27 frames are being saved to U:\krios\20190717\PilA_1655.tif 27 frames are being saved to U:\krios\20190717\PilA_1656.tif 27 frames are being saved to U:\krios\20190717\PilA_1657.tif Running script 17 at item 345 (489.58, -519.39) Remaining time to fill dewars is 3720 Dewars are NOT busy filling Trimming A by 24 84 135 20, reference by 200 24 20 220 Trimming A by 24 84 135 20, reference by 180 23 145 20 Trimming A by 24 84 135 20, reference by 20 145 20 170 Trimming A by 24 84 135 20, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.19 -0.23 Trimming A by 20 20 20 20, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.16 -0.59 (2nd move error 0.02 -0.38) Finished aligning to item in 2 rounds -26.6 -3.7 unbinned pixels; 21.3 101.2 nm along two shift axes; 19.8 -102.7 nm on specimen axes Total_Shift = 53.685513 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.16 microns changed by -2.46 to target drift = 0.19 nm/sec Measured defocus = -2.36 microns changed by 0.06 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1658.tif 27 frames are being saved to U:\krios\20190717\PilA_1659.tif 27 frames are being saved to U:\krios\20190717\PilA_1660.tif 27 frames are being saved to U:\krios\20190717\PilA_1661.tif 27 frames are being saved to U:\krios\20190717\PilA_1662.tif 27 frames are being saved to U:\krios\20190717\PilA_1663.tif Running script 17 at item 346 (492.03, -521.18) Remaining time to fill dewars is 3480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 25 20, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.16 -0.50 (2nd move error 0.02 -0.30) Finished aligning to item in 1 rounds Trimming A by 21 25 25 20, reference by 20 20 25 20 -24.9 -3.5 unbinned pixels; 19.8 94.8 nm along two shift axes; 18.7 -96.1 nm on specimen axes Total_Shift = 50.259537 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.58 microns changed by -3.98 to target drift = 0.15 nm/sec Measured defocus = -2.51 microns changed by 0.11 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1664.tif 27 frames are being saved to U:\krios\20190717\PilA_1665.tif 27 frames are being saved to U:\krios\20190717\PilA_1666.tif 27 frames are being saved to U:\krios\20190717\PilA_1667.tif 27 frames are being saved to U:\krios\20190717\PilA_1668.tif 27 frames are being saved to U:\krios\20190717\PilA_1669.tif Running script 17 at item 347 (494.55, -522.95) Remaining time to fill dewars is 3360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 52 120 20, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.15 -0.31 (2nd move error 0.04 -0.11) Finished aligning to item in 1 rounds Trimming A by 21 51 120 20, reference by 22 52 120 20 -14.8 -2.7 unbinned pixels; 9.3 57.2 nm along two shift axes; 13.7 -56.8 nm on specimen axes Total_Shift = 30.009499 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.17 microns changed by -2.67 to target drift = 0.15 nm/sec Measured defocus = -2.48 microns changed by -0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1670.tif 27 frames are being saved to U:\krios\20190717\PilA_1671.tif 27 frames are being saved to U:\krios\20190717\PilA_1672.tif 27 frames are being saved to U:\krios\20190717\PilA_1673.tif 27 frames are being saved to U:\krios\20190717\PilA_1674.tif 27 frames are being saved to U:\krios\20190717\PilA_1675.tif Running script 17 at item 348 (497.09, -524.68) Remaining time to fill dewars is 3180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 160 160 20, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.11 -0.18 (2nd move error 0.07 0.02) Finished aligning to item in 1 rounds Trimming A by 24 147 175 20, reference by 24 160 160 20 -0.2 -1.3 unbinned pixels; 4.7 2.7 nm along two shift axes; 5.3 -0.4 nm on specimen axes Total_Shift = 2.724738 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.26 microns changed by -2.86 to target drift = 0.15 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1676.tif 27 frames are being saved to U:\krios\20190717\PilA_1677.tif 27 frames are being saved to U:\krios\20190717\PilA_1678.tif 27 frames are being saved to U:\krios\20190717\PilA_1679.tif 27 frames are being saved to U:\krios\20190717\PilA_1680.tif 27 frames are being saved to U:\krios\20190717\PilA_1681.tif Running script 17 at item 349 (492.87, -525.53) Remaining time to fill dewars is 2940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 95 75 20, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.03 -0.47 (2nd move error 0.15 -0.27) Finished aligning to item in 1 rounds Trimming A by 23 105 75 20, reference by 24 95 75 20 -21.3 -5.3 unbinned pixels; 8.4 84.5 nm along two shift axes; 24.9 -81.6 nm on specimen axes Total_Shift = 43.813608 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.66 microns changed by -2.76 to target drift = 0.19 nm/sec Measured defocus = -1.07 microns changed by -0.03 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1682.tif 27 frames are being saved to U:\krios\20190717\PilA_1683.tif 27 frames are being saved to U:\krios\20190717\PilA_1684.tif 27 frames are being saved to U:\krios\20190717\PilA_1685.tif 27 frames are being saved to U:\krios\20190717\PilA_1686.tif 27 frames are being saved to U:\krios\20190717\PilA_1687.tif Running script 17 at item 350 (490.38, -523.78) Remaining time to fill dewars is 2760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 55 20 50, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.10 -0.57 (2nd move error 0.09 -0.36) Finished aligning to item in 1 rounds Trimming A by 20 60 20 55, reference by 20 55 20 50 -24.7 -4.5 unbinned pixels; 15.7 95.6 nm along two shift axes; 22.6 -95.1 nm on specimen axes Total_Shift = 50.190605 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.79 microns changed by -3.99 to target drift = 0.16 nm/sec Measured defocus = -1.26 microns changed by 0.06 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1688.tif 27 frames are being saved to U:\krios\20190717\PilA_1689.tif 27 frames are being saved to U:\krios\20190717\PilA_1690.tif 27 frames are being saved to U:\krios\20190717\PilA_1691.tif 27 frames are being saved to U:\krios\20190717\PilA_1692.tif 27 frames are being saved to U:\krios\20190717\PilA_1693.tif Running script 17 at item 351 (487.96, -522.42) Remaining time to fill dewars is 2640 Dewars are NOT busy filling Trimming A by 20 145 20 170, reference by 200 24 20 220 Trimming A by 20 145 20 170, reference by 180 23 145 20 Trimming A by 20 145 20 170, reference by 20 145 20 170 Trimming A by 20 145 20 170, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.21 -0.27 Trimming A by 21 55 20 60, reference by 20 145 20 170 Disparity in stage position after aligning to target position: -0.07 0.04 (2nd move error 0.14 0.28) Finished aligning to item in 2 rounds Trimming A by 21 50 35 50, reference by 21 55 20 60 0.9 -1.3 unbinned pixels; 6.1 1.1 nm along two shift axes; 5.0 3.7 nm on specimen axes Total_Shift = 3.20794 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.26 microns changed by -2.56 to target drift = 0.14 nm/sec Measured defocus = -1.34 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1694.tif 27 frames are being saved to U:\krios\20190717\PilA_1695.tif 27 frames are being saved to U:\krios\20190717\PilA_1696.tif 27 frames are being saved to U:\krios\20190717\PilA_1697.tif 27 frames are being saved to U:\krios\20190717\PilA_1698.tif 27 frames are being saved to U:\krios\20190717\PilA_1699.tif Running script 17 at item 352 (485.49, -520.86) Remaining time to fill dewars is 2460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 50 20 65, reference by 20 145 20 170 Disparity in stage position after aligning to target position: -0.10 -0.19 (2nd move error 0.11 0.05) Finished aligning to item in 1 rounds Trimming A by 21 50 20 60, reference by 21 50 20 65 -2.1 -1.9 unbinned pixels; 4.2 10.6 nm along two shift axes; 7.9 -8.0 nm on specimen axes Total_Shift = 5.747595 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.25 microns changed by -3.65 to target drift = 0.14 nm/sec Measured defocus = -1.45 microns changed by 0.05 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1700.tif 27 frames are being saved to U:\krios\20190717\PilA_1701.tif 27 frames are being saved to U:\krios\20190717\PilA_1702.tif 27 frames are being saved to U:\krios\20190717\PilA_1703.tif 27 frames are being saved to U:\krios\20190717\PilA_1704.tif 27 frames are being saved to U:\krios\20190717\PilA_1705.tif Running script 17 at item 353 (482.95, -519.10) Remaining time to fill dewars is 2280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 47 20 20 130, reference by 20 145 20 170 Disparity in stage position after aligning to target position: -0.26 -0.26 (2nd move error -0.04 -0.02) Finished aligning to item in 1 rounds Trimming A by 47 20 20 130, reference by 47 20 20 130 -2.5 9.6 unbinned pixels; 38.4 5.6 nm along two shift axes; -36.7 -11.6 nm on specimen axes Total_Shift = 19.75915 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.64 microns changed by -2.14 to target drift = 0.05 nm/sec Measured defocus = -1.48 microns changed by -0.02 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1706.tif 27 frames are being saved to U:\krios\20190717\PilA_1707.tif 27 frames are being saved to U:\krios\20190717\PilA_1708.tif 27 frames are being saved to U:\krios\20190717\PilA_1709.tif 27 frames are being saved to U:\krios\20190717\PilA_1710.tif 27 frames are being saved to U:\krios\20190717\PilA_1711.tif Running script 17 at item 354 (481.32, -521.71) Remaining time to fill dewars is 2040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 100 20 165, reference by 20 145 20 170 Disparity in stage position after aligning to target position: -0.13 -0.32 (2nd move error 0.08 -0.08) Finished aligning to item in 1 rounds Trimming A by 21 100 20 165, reference by 21 100 20 165 -9.3 -5.8 unbinned pixels; 9.1 42.2 nm along two shift axes; 24.4 -35.0 nm on specimen axes Total_Shift = 21.896726 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.92 microns changed by -3.52 to target drift = 0.12 nm/sec Measured defocus = -1.66 microns changed by 0.06 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1712.tif 27 frames are being saved to U:\krios\20190717\PilA_1713.tif 27 frames are being saved to U:\krios\20190717\PilA_1714.tif 27 frames are being saved to U:\krios\20190717\PilA_1715.tif 27 frames are being saved to U:\krios\20190717\PilA_1716.tif 27 frames are being saved to U:\krios\20190717\PilA_1717.tif Running script 17 at item 355 (483.83, -523.47) Remaining time to fill dewars is 1920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 120 20 135, reference by 20 145 20 170 Disparity in stage position after aligning to target position: -0.04 -0.23 (2nd move error 0.17 0.01) Finished aligning to item in 1 rounds Trimming A by 24 115 20 135, reference by 23 120 20 135 3.8 -5.8 unbinned pixels; 26.3 5.0 nm along two shift axes; 21.8 16.1 nm on specimen axes Total_Shift = 13.880792 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.60 microns changed by -2.30 to target drift = 0.14 nm/sec Measured defocus = -1.67 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1718.tif 27 frames are being saved to U:\krios\20190717\PilA_1719.tif 27 frames are being saved to U:\krios\20190717\PilA_1720.tif 27 frames are being saved to U:\krios\20190717\PilA_1721.tif 27 frames are being saved to U:\krios\20190717\PilA_1722.tif 27 frames are being saved to U:\krios\20190717\PilA_1723.tif Running script 17 at item 356 (486.36, -525.23) Remaining time to fill dewars is 1860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 135 75 120, reference by 20 145 20 170 Disparity in stage position after aligning to target position: 0.03 -0.10 (2nd move error 0.25 0.13) Finished aligning to item in 1 rounds Trimming A by 21 130 70 115, reference by 21 135 75 120 5.4 0.8 unbinned pixels; 3.9 20.6 nm along two shift axes; -4.4 20.7 nm on specimen axes Total_Shift = 10.876895 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.98 microns changed by -3.78 to target drift = 0.11 nm/sec Measured defocus = -1.85 microns changed by 0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1724.tif 27 frames are being saved to U:\krios\20190717\PilA_1725.tif 27 frames are being saved to U:\krios\20190717\PilA_1726.tif 27 frames are being saved to U:\krios\20190717\PilA_1727.tif 27 frames are being saved to U:\krios\20190717\PilA_1728.tif 27 frames are being saved to U:\krios\20190717\PilA_1729.tif Running script 17 at item 357 (488.73, -526.65) Remaining time to fill dewars is 1620 Dewars are NOT busy filling Trimming A by 24 150 20 170, reference by 200 24 20 220 Trimming A by 24 150 20 170, reference by 180 23 145 20 Trimming A by 24 150 20 170, reference by 20 145 20 170 Trimming A by 24 150 20 170, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.15 -0.26 Trimming A by 20 140 20 115, reference by 20 145 20 170 Disparity in stage position after aligning to target position: 0.03 0.13 (2nd move error 0.16 0.36) Finished aligning to item in 2 rounds Trimming A by 21 135 75 100, reference by 20 140 20 115 3.0 2.4 unbinned pixels; 5.0 14.6 nm along two shift axes; -10.1 11.3 nm on specimen axes Total_Shift = 7.794703 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.68 microns changed by -2.58 to target drift = 0.13 nm/sec Measured defocus = -1.88 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1730.tif 27 frames are being saved to U:\krios\20190717\PilA_1731.tif 27 frames are being saved to U:\krios\20190717\PilA_1732.tif 27 frames are being saved to U:\krios\20190717\PilA_1733.tif 27 frames are being saved to U:\krios\20190717\PilA_1734.tif 27 frames are being saved to U:\krios\20190717\PilA_1735.tif Running script 17 at item 358 (491.28, -528.00) Remaining time to fill dewars is 1500 Dewars are NOT busy filling Trimming A by 24 84 135 20, reference by 200 24 20 220 Trimming A by 24 84 135 20, reference by 180 23 145 20 Trimming A by 24 84 135 20, reference by 20 145 20 170 Trimming A by 24 84 135 20, reference by 21 82 120 20 Disparity in stage position after aligning to center of map frame: -0.17 -0.20 Trimming A by 20 150 50 95, reference by 21 82 120 20 Disparity in stage position after aligning to target position: -0.10 -0.47 (2nd move error 0.07 -0.29) Finished aligning to item in 2 rounds Trimming A by 20 160 50 85, reference by 20 150 50 95 -22.5 -8.9 unbinned pixels; 3.4 94.3 nm along two shift axes; 39.4 -85.5 nm on specimen axes Total_Shift = 48.334358 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 2.13 microns changed by -4.13 to target drift = 0.16 nm/sec Measured defocus = -2.11 microns changed by 0.11 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1736.tif 27 frames are being saved to U:\krios\20190717\PilA_1737.tif 27 frames are being saved to U:\krios\20190717\PilA_1738.tif 27 frames are being saved to U:\krios\20190717\PilA_1739.tif 27 frames are being saved to U:\krios\20190717\PilA_1740.tif 27 frames are being saved to U:\krios\20190717\PilA_1741.tif Running script 17 at item 359 (549.26, -547.37) Remaining time to fill dewars is 1200 Dewars are NOT busy filling Trimming A by 170 24 140 20, reference by 210 24 20 270 Trimming A by 170 24 140 20, reference by 155 22 120 20 Trimming A by 170 24 140 20, reference by 20 245 20 265 Trimming A by 170 24 140 20, reference by 21 200 125 20 Disparity in stage position after aligning to center of map frame: -0.34 -0.25 Trimming A by 95 26 140 20, reference by 155 22 120 20 Disparity in stage position after aligning to target position: -0.33 -0.10 (2nd move error 0.00 0.12) Finished aligning to item in 2 rounds Trimming A by 95 21 145 20, reference by 95 26 140 20 -2.4 2.9 unbinned pixels; 13.7 4.4 nm along two shift axes; -10.6 -10.0 nm on specimen axes Total_Shift = 7.486926 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.58 microns changed by -2.68 to target drift = 0.11 nm/sec Measured defocus = -2.10 microns changed by 0.00 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1742.tif 27 frames are being saved to U:\krios\20190717\PilA_1743.tif 27 frames are being saved to U:\krios\20190717\PilA_1744.tif 27 frames are being saved to U:\krios\20190717\PilA_1745.tif 27 frames are being saved to U:\krios\20190717\PilA_1746.tif 27 frames are being saved to U:\krios\20190717\PilA_1747.tif Running script 17 at item 360 (546.80, -545.68) Remaining time to fill dewars is 1020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 110 23 105 20, reference by 155 22 120 20 Disparity in stage position after aligning to target position: -0.27 -0.26 (2nd move error 0.06 -0.04) Finished aligning to item in 1 rounds Trimming A by 110 23 105 20, reference by 110 23 105 20 -3.4 -0.0 unbinned pixels; 4.3 12.1 nm along two shift axes; 0.8 -13.1 nm on specimen axes Total_Shift = 6.719747 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.62 microns changed by -3.82 to target drift = 0.10 nm/sec Measured defocus = -2.26 microns changed by 0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1748.tif 27 frames are being saved to U:\krios\20190717\PilA_1749.tif 27 frames are being saved to U:\krios\20190717\PilA_1750.tif 27 frames are being saved to U:\krios\20190717\PilA_1751.tif 27 frames are being saved to U:\krios\20190717\PilA_1752.tif 27 frames are being saved to U:\krios\20190717\PilA_1753.tif Running script 17 at item 361 (544.32, -543.91) Remaining time to fill dewars is 900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 24 85 20, reference by 155 22 120 20 Disparity in stage position after aligning to target position: -0.36 -0.37 (2nd move error -0.02 -0.15) Finished aligning to item in 1 rounds Trimming A by 135 24 85 20, reference by 135 24 85 20 -16.8 6.2 unbinned pixels; 44.9 51.1 nm along two shift axes; -20.8 -66.7 nm on specimen axes Total_Shift = 35.837414 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.01 microns changed by -2.29 to target drift = 0.05 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1754.tif 27 frames are being saved to U:\krios\20190717\PilA_1755.tif 27 frames are being saved to U:\krios\20190717\PilA_1756.tif 27 frames are being saved to U:\krios\20190717\PilA_1757.tif 27 frames are being saved to U:\krios\20190717\PilA_1758.tif 27 frames are being saved to U:\krios\20190717\PilA_1759.tif Running script 17 at item 362 (541.82, -542.19) Remaining time to fill dewars is 720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 180 20 30 90, reference by 155 22 120 20 Disparity in stage position after aligning to target position: -0.43 -0.49 (2nd move error -0.10 -0.27) Finished aligning to item in 1 rounds Trimming A by 180 20 20 90, reference by 180 20 30 90 -31.8 17.8 unbinned pixels; 107.0 87.9 nm along two shift axes; -62.5 -127.6 nm on specimen axes Total_Shift = 72.960735 Trimming A by 170 20 30 95, reference by 180 20 30 90 0.1 10.7 unbinned pixels; 39.2 16.3 nm along two shift axes; -41.6 -2.0 nm on specimen axes Total_Shift = 21.358266 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.01 microns changed by -3.41 to target drift = 0.01 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1760.tif 27 frames are being saved to U:\krios\20190717\PilA_1761.tif 27 frames are being saved to U:\krios\20190717\PilA_1762.tif 27 frames are being saved to U:\krios\20190717\PilA_1763.tif 27 frames are being saved to U:\krios\20190717\PilA_1764.tif 27 frames are being saved to U:\krios\20190717\PilA_1765.tif Running script 17 at item 363 (539.92, -545.28) Remaining time to fill dewars is 600 Dewars are NOT busy filling Trimming A by 215 24 20 275, reference by 210 24 20 270 Trimming A by 215 24 20 275, reference by 155 22 120 20 Trimming A by 215 24 20 275, reference by 20 245 20 265 Trimming A by 215 24 20 275, reference by 21 200 125 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.16 Trimming A by 125 22 20 110, reference by 210 24 20 270 Disparity in stage position after aligning to target position: -0.06 0.12 (2nd move error -0.09 0.26) Finished aligning to item in 2 rounds Trimming A by 117 22 20 130, reference by 125 22 20 110 0.0 19.0 unbinned pixels; 70.0 28.7 nm along two shift axes; -74.1 -3.9 nm on specimen axes Total_Shift = 38.084492 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.47 microns changed by -2.03 to target drift = 0.11 nm/sec Measured defocus = -2.52 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1766.tif 27 frames are being saved to U:\krios\20190717\PilA_1767.tif 27 frames are being saved to U:\krios\20190717\PilA_1768.tif 27 frames are being saved to U:\krios\20190717\PilA_1769.tif 27 frames are being saved to U:\krios\20190717\PilA_1770.tif 27 frames are being saved to U:\krios\20190717\PilA_1771.tif Running script 17 at item 364 (542.70, -546.49) Remaining time to fill dewars is 420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 20 20 50, reference by 155 22 120 20 Disparity in stage position after aligning to target position: -0.26 -0.45 (2nd move error 0.07 -0.23) Finished aligning to item in 1 rounds Trimming A by 60 20 20 50, reference by 65 20 20 50 -26.1 -4.7 unbinned pixels; 16.9 101.1 nm along two shift axes; 23.7 -100.7 nm on specimen axes Total_Shift = 53.091794 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.97 microns changed by -3.57 to target drift = 0.17 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1772.tif 27 frames are being saved to U:\krios\20190717\PilA_1773.tif 27 frames are being saved to U:\krios\20190717\PilA_1774.tif 27 frames are being saved to U:\krios\20190717\PilA_1775.tif 27 frames are being saved to U:\krios\20190717\PilA_1776.tif 27 frames are being saved to U:\krios\20190717\PilA_1777.tif Running script 17 at item 365 (545.15, -548.19) Remaining time to fill dewars is 240 Dewars are NOT busy filling Trimming A by 170 23 135 20, reference by 210 24 20 270 Trimming A by 170 23 135 20, reference by 155 22 120 20 Trimming A by 170 23 135 20, reference by 20 245 20 265 Trimming A by 170 23 135 20, reference by 21 200 125 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.22 Trimming A by 40 21 45 20, reference by 155 22 120 20 Disparity in stage position after aligning to target position: -0.27 -0.33 (2nd move error 0.01 -0.12) Finished aligning to item in 2 rounds Trimming A by 40 21 40 20, reference by 40 21 45 20 -14.1 -0.7 unbinned pixels; 16.0 51.9 nm along two shift axes; 5.6 -54.9 nm on specimen axes Total_Shift = 28.32007 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.48 microns changed by -2.58 to target drift = 0.07 nm/sec Measured defocus = -1.15 microns changed by 0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1778.tif 27 frames are being saved to U:\krios\20190717\PilA_1779.tif 27 frames are being saved to U:\krios\20190717\PilA_1780.tif 27 frames are being saved to U:\krios\20190717\PilA_1781.tif 27 frames are being saved to U:\krios\20190717\PilA_1782.tif 27 frames are being saved to U:\krios\20190717\PilA_1783.tif Running script 17 at item 366 (548.21, -549.77) Remaining time to fill dewars is 60 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 66 120 20, reference by 155 22 120 20 Disparity in stage position after aligning to target position: -0.24 -0.10 (2nd move error 0.03 0.10) Finished aligning to item in 1 rounds Trimming A by 21 41 135 20, reference by 20 66 120 20 -1.5 0.5 unbinned pixels; 3.8 4.7 nm along two shift axes; -1.6 -6.0 nm on specimen axes Total_Shift = 3.186355 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 3.18 microns changed by -4.38 to target drift = 0.19 nm/sec Measured defocus = -0.29 microns changed by -0.91 to target drift = 0.44 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1784.tif 27 frames are being saved to U:\krios\20190717\PilA_1785.tif 27 frames are being saved to U:\krios\20190717\PilA_1786.tif 27 frames are being saved to U:\krios\20190717\PilA_1787.tif 27 frames are being saved to U:\krios\20190717\PilA_1788.tif 27 frames are being saved to U:\krios\20190717\PilA_1789.tif Running script 17 at item 367 (550.30, -551.69) Remaining time to fill dewars is 4140 Dewars ARE busy filling Dewars ARE busy filling Dewars are NOT busy filling Trimming A by 21 210 135 20, reference by 210 24 20 270 Trimming A by 21 210 135 20, reference by 155 22 120 20 Trimming A by 21 210 135 20, reference by 20 245 20 265 Trimming A by 21 210 135 20, reference by 21 200 125 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.25 Trimming A by 23 110 195 20, reference by 21 200 125 20 Disparity in stage position after aligning to target position: -0.26 -0.10 (2nd move error -0.02 0.13) Finished aligning to item in 2 rounds Trimming A by 21 106 195 20, reference by 23 110 195 20 -3.6 5.8 unbinned pixels; 25.9 4.1 nm along two shift axes; -21.7 -15.1 nm on specimen axes Total_Shift = 13.585587 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.59 microns changed by -2.89 to target drift = 0.10 nm/sec Measured defocus = -1.30 microns changed by -0.00 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1790.tif 27 frames are being saved to U:\krios\20190717\PilA_1791.tif 27 frames are being saved to U:\krios\20190717\PilA_1792.tif 27 frames are being saved to U:\krios\20190717\PilA_1793.tif 27 frames are being saved to U:\krios\20190717\PilA_1794.tif 27 frames are being saved to U:\krios\20190717\PilA_1795.tif Running script 17 at item 368 (548.66, -554.21) Remaining time to fill dewars is 31440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 165 150 20, reference by 21 200 125 20 Disparity in stage position after aligning to target position: -0.35 -0.20 (2nd move error -0.11 0.02) Finished aligning to item in 1 rounds Trimming A by 20 165 150 20, reference by 20 165 150 20 -1.7 21.9 unbinned pixels; 82.8 26.8 nm along two shift axes; -84.9 -11.3 nm on specimen axes Total_Shift = 43.965629 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.88 microns changed by -4.28 to target drift = 0.12 nm/sec Measured defocus = -1.44 microns changed by 0.04 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1796.tif 27 frames are being saved to U:\krios\20190717\PilA_1797.tif 27 frames are being saved to U:\krios\20190717\PilA_1798.tif 27 frames are being saved to U:\krios\20190717\PilA_1799.tif 27 frames are being saved to U:\krios\20190717\PilA_1800.tif 27 frames are being saved to U:\krios\20190717\PilA_1801.tif Running script 17 at item 369 (546.20, -552.45) Remaining time to fill dewars is 31380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 95 80 20, reference by 21 200 125 20 Disparity in stage position after aligning to target position: -0.28 -0.35 (2nd move error -0.04 -0.12) Finished aligning to item in 1 rounds Trimming A by 24 95 80 20, reference by 23 95 80 20 -15.5 8.4 unbinned pixels; 51.0 43.1 nm along two shift axes; -29.3 -62.0 nm on specimen axes Total_Shift = 35.200089 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.20 microns changed by -2.70 to target drift = 0.03 nm/sec Measured defocus = -1.49 microns changed by -0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1802.tif 27 frames are being saved to U:\krios\20190717\PilA_1803.tif 27 frames are being saved to U:\krios\20190717\PilA_1804.tif 27 frames are being saved to U:\krios\20190717\PilA_1805.tif 27 frames are being saved to U:\krios\20190717\PilA_1806.tif 27 frames are being saved to U:\krios\20190717\PilA_1807.tif Running script 17 at item 370 (543.66, -550.75) Remaining time to fill dewars is 31260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 30 20 25, reference by 21 200 125 20 Disparity in stage position after aligning to target position: -0.36 -0.44 (2nd move error -0.11 -0.22) Finished aligning to item in 1 rounds Trimming A by 20 40 20 20, reference by 20 30 20 25 -26.6 20.8 unbinned pixels; 111.2 64.3 nm along two shift axes; -75.3 -107.8 nm on specimen axes Total_Shift = 67.49076 Trimming A by 20 40 20 20, reference by 20 30 20 25 -3.4 11.3 unbinned pixels; 46.1 5.0 nm along two shift axes; -43.4 -15.5 nm on specimen axes Total_Shift = 23.670465 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.29 microns changed by -3.89 to target drift = 0.02 nm/sec Measured defocus = -1.70 microns changed by 0.10 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1808.tif 27 frames are being saved to U:\krios\20190717\PilA_1809.tif 27 frames are being saved to U:\krios\20190717\PilA_1810.tif 27 frames are being saved to U:\krios\20190717\PilA_1811.tif 27 frames are being saved to U:\krios\20190717\PilA_1812.tif 27 frames are being saved to U:\krios\20190717\PilA_1813.tif Running script 17 at item 371 (541.01, -549.03) Remaining time to fill dewars is 31140 Dewars are NOT busy filling Trimming A by 170 23 135 20, reference by 210 24 20 270 Trimming A by 170 23 135 20, reference by 155 22 120 20 Trimming A by 170 23 135 20, reference by 20 245 20 265 Trimming A by 170 23 135 20, reference by 21 200 125 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.26 Trimming A by 40 21 20 60, reference by 155 22 120 20 Disparity in stage position after aligning to target position: -0.25 -0.56 (2nd move error -0.03 -0.34) Finished aligning to item in 2 rounds Trimming A by 20 20 20 70, reference by 40 21 20 60 -30.0 5.6 unbinned pixels; 59.9 99.5 nm along two shift axes; -15.6 -117.9 nm on specimen axes Total_Shift = 61.041288 Trimming A by 25 22 20 65, reference by 40 21 20 60 0.2 3.7 unbinned pixels; 13.5 6.2 nm along two shift axes; -14.5 -0.2 nm on specimen axes Total_Shift = 7.452706 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.67 microns changed by -2.37 to target drift = 0.04 nm/sec Measured defocus = -1.75 microns changed by 0.05 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1814.tif 27 frames are being saved to U:\krios\20190717\PilA_1815.tif 27 frames are being saved to U:\krios\20190717\PilA_1816.tif 27 frames are being saved to U:\krios\20190717\PilA_1817.tif 27 frames are being saved to U:\krios\20190717\PilA_1818.tif 27 frames are being saved to U:\krios\20190717\PilA_1819.tif Running script 17 at item 372 (538.50, -547.95) Remaining time to fill dewars is 31020 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 215 24 20 275, reference by 210 24 20 270 Trimming A by 215 24 20 275, reference by 155 22 120 20 Trimming A by 215 24 20 275, reference by 20 245 20 265 Trimming A by 215 24 20 275, reference by 21 200 125 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.20 Trimming A by 60 21 20 155, reference by 210 24 20 270 Disparity in stage position after aligning to target position: -0.14 0.00 (2nd move error 0.14 0.18) Finished aligning to item in 2 rounds Trimming A by 64 21 20 150, reference by 60 21 20 155 0.3 3.1 unbinned pixels; 11.1 5.8 nm along two shift axes; -12.3 0.5 nm on specimen axes Total_Shift = 6.303505 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.73 microns changed by -3.53 to target drift = 0.12 nm/sec Measured defocus = -1.88 microns changed by 0.08 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1820.tif 27 frames are being saved to U:\krios\20190717\PilA_1821.tif 27 frames are being saved to U:\krios\20190717\PilA_1822.tif 27 frames are being saved to U:\krios\20190717\PilA_1823.tif 27 frames are being saved to U:\krios\20190717\PilA_1824.tif 27 frames are being saved to U:\krios\20190717\PilA_1825.tif Running script 17 at item 373 (537.14, -550.53) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 50 62 20 160, reference by 210 24 20 270 Disparity in stage position after aligning to target position: -0.14 -0.13 (2nd move error 0.13 0.05) Finished aligning to item in 1 rounds Trimming A by 58 66 20 150, reference by 50 62 20 160 0.5 -1.4 unbinned pixels; 5.8 0.5 nm along two shift axes; 5.4 2.1 nm on specimen axes Total_Shift = 2.992682 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.20 microns changed by -2.10 to target drift = 0.13 nm/sec Measured defocus = -1.91 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1826.tif 27 frames are being saved to U:\krios\20190717\PilA_1827.tif 27 frames are being saved to U:\krios\20190717\PilA_1828.tif 27 frames are being saved to U:\krios\20190717\PilA_1829.tif 27 frames are being saved to U:\krios\20190717\PilA_1830.tif 27 frames are being saved to U:\krios\20190717\PilA_1831.tif Running script 17 at item 374 (539.50, -552.22) Remaining time to fill dewars is 30720 Dewars are NOT busy filling Trimming A by 22 241 20 270, reference by 210 24 20 270 Trimming A by 22 241 20 270, reference by 155 22 120 20 Trimming A by 22 241 20 270, reference by 20 245 20 265 Trimming A by 22 241 20 270, reference by 21 200 125 20 Disparity in stage position after aligning to center of map frame: -0.21 -0.22 Trimming A by 20 95 20 115, reference by 20 245 20 265 Disparity in stage position after aligning to target position: -0.14 0.06 (2nd move error 0.06 0.25) Finished aligning to item in 2 rounds Trimming A by 21 85 20 115, reference by 20 95 20 115 1.1 -2.5 unbinned pixels; 10.7 0.3 nm along two shift axes; 9.6 4.9 nm on specimen axes Total_Shift = 5.525352 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.70 microns changed by -3.70 to target drift = 0.16 nm/sec Measured defocus = -2.09 microns changed by 0.09 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1832.tif 27 frames are being saved to U:\krios\20190717\PilA_1833.tif 27 frames are being saved to U:\krios\20190717\PilA_1834.tif 27 frames are being saved to U:\krios\20190717\PilA_1835.tif 27 frames are being saved to U:\krios\20190717\PilA_1836.tif 27 frames are being saved to U:\krios\20190717\PilA_1837.tif Running script 17 at item 375 (542.02, -553.28) Remaining time to fill dewars is 30660 Dewars are NOT busy filling Trimming A by 20 209 140 20, reference by 210 24 20 270 Trimming A by 20 209 140 20, reference by 155 22 120 20 Trimming A by 20 209 140 20, reference by 20 245 20 265 Trimming A by 20 209 140 20, reference by 21 200 125 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.20 Trimming A by 21 100 55 80, reference by 21 200 125 20 Disparity in stage position after aligning to target position: -0.39 -0.56 (2nd move error -0.14 -0.39) Finished aligning to item in 2 rounds Trimming A by 24 105 20 90, reference by 21 100 55 80 -31.4 26.3 unbinned pixels; 137.5 73.5 nm along two shift axes; -95.5 -127.6 nm on specimen axes Total_Shift = 81.839437 Trimming A by 23 110 20 90, reference by 21 100 55 80 1.9 17.2 unbinned pixels; 60.9 32.9 nm along two shift axes; -67.5 3.9 nm on specimen axes Total_Shift = 34.69042 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.42 microns changed by -2.52 to target drift = 0.01 nm/sec Measured defocus = -2.10 microns changed by 0.00 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1838.tif 27 frames are being saved to U:\krios\20190717\PilA_1839.tif 27 frames are being saved to U:\krios\20190717\PilA_1840.tif 27 frames are being saved to U:\krios\20190717\PilA_1841.tif 27 frames are being saved to U:\krios\20190717\PilA_1842.tif 27 frames are being saved to U:\krios\20190717\PilA_1843.tif Running script 17 at item 376 (610.15, -437.11) Remaining time to fill dewars is 30540 Dewars are NOT busy filling Trimming A by 20 155 165 20, reference by 286 22 20 290 Trimming A by 20 155 165 20, reference by 275 23 175 20 Trimming A by 20 155 165 20, reference by 22 165 20 250 Trimming A by 20 155 165 20, reference by 23 151 130 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.18 Trimming A by 48 46 185 20, reference by 23 151 130 20 Disparity in stage position after aligning to target position: -0.46 -0.12 (2nd move error -0.09 0.04) Finished aligning to item in 2 rounds Trimming A by 62 57 175 20, reference by 48 46 185 20 1.3 16.9 unbinned pixels; 60.5 30.1 nm along two shift axes; -66.1 1.5 nm on specimen axes Total_Shift = 33.934989 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.74 microns changed by -3.94 to target drift = 0.19 nm/sec Measured defocus = -2.21 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1844.tif 27 frames are being saved to U:\krios\20190717\PilA_1845.tif 27 frames are being saved to U:\krios\20190717\PilA_1846.tif 27 frames are being saved to U:\krios\20190717\PilA_1847.tif 27 frames are being saved to U:\krios\20190717\PilA_1848.tif 27 frames are being saved to U:\krios\20190717\PilA_1849.tif Running script 17 at item 377 (607.63, -435.33) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 105 20 115 20, reference by 23 151 130 20 Disparity in stage position after aligning to target position: -0.54 -0.28 (2nd move error -0.15 -0.12) Finished aligning to item in 1 rounds Trimming A by 100 20 115 20, reference by 105 20 115 20 -12.2 27.4 unbinned pixels; 116.8 2.6 nm along two shift axes; -104.1 -53.2 nm on specimen axes Total_Shift = 60.02954 Trimming A by 100 20 115 20, reference by 105 20 115 20 -4.6 17.9 unbinned pixels; 71.9 10.4 nm along two shift axes; -68.8 -21.7 nm on specimen axes Total_Shift = 37.015475 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.14 microns changed by -2.44 to target drift = 0.03 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1850.tif 27 frames are being saved to U:\krios\20190717\PilA_1851.tif 27 frames are being saved to U:\krios\20190717\PilA_1852.tif 27 frames are being saved to U:\krios\20190717\PilA_1853.tif 27 frames are being saved to U:\krios\20190717\PilA_1854.tif 27 frames are being saved to U:\krios\20190717\PilA_1855.tif Running script 17 at item 378 (604.86, -433.69) Remaining time to fill dewars is 30300 Dewars are NOT busy filling Trimming A by 288 20 200 20, reference by 286 22 20 290 Trimming A by 288 20 200 20, reference by 275 23 175 20 Trimming A by 288 20 200 20, reference by 22 165 20 250 Trimming A by 288 20 200 20, reference by 23 151 130 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.17 Trimming A by 125 21 80 20, reference by 275 23 175 20 Disparity in stage position after aligning to target position: -0.33 -0.35 (2nd move error 0.04 -0.20) Finished aligning to item in 2 rounds Trimming A by 120 20 80 20, reference by 125 21 80 20 -22.8 -2.7 unbinned pixels; 19.8 86.4 nm along two shift axes; 15.5 -88.3 nm on specimen axes Total_Shift = 46.017738 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.20 microns changed by -3.60 to target drift = 0.12 nm/sec Measured defocus = -2.46 microns changed by 0.06 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1856.tif 27 frames are being saved to U:\krios\20190717\PilA_1857.tif 27 frames are being saved to U:\krios\20190717\PilA_1858.tif 27 frames are being saved to U:\krios\20190717\PilA_1859.tif 27 frames are being saved to U:\krios\20190717\PilA_1860.tif 27 frames are being saved to U:\krios\20190717\PilA_1861.tif Running script 17 at item 379 (605.88, -438.03) Remaining time to fill dewars is 30120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 21 70 20, reference by 23 151 130 20 Disparity in stage position after aligning to target position: -0.44 -0.32 (2nd move error -0.06 -0.17) Finished aligning to item in 1 rounds Trimming A by 21 21 70 20, reference by 21 21 70 20 -19.1 12.9 unbinned pixels; 72.2 49.3 nm along two shift axes; -46.0 -77.0 nm on specimen axes Total_Shift = 46.034371 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.03 microns changed by -2.53 to target drift = 0.02 nm/sec Measured defocus = -2.57 microns changed by 0.07 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1862.tif 27 frames are being saved to U:\krios\20190717\PilA_1863.tif 27 frames are being saved to U:\krios\20190717\PilA_1864.tif 27 frames are being saved to U:\krios\20190717\PilA_1865.tif 27 frames are being saved to U:\krios\20190717\PilA_1866.tif 27 frames are being saved to U:\krios\20190717\PilA_1867.tif Running script 17 at item 380 (608.41, -439.70) Remaining time to fill dewars is 30000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 77 145 20, reference by 23 151 130 20 Disparity in stage position after aligning to target position: -0.37 -0.24 (2nd move error 0.01 -0.08) Finished aligning to item in 1 rounds Trimming A by 22 77 145 20, reference by 22 77 145 20 -11.0 -0.9 unbinned pixels; 11.1 40.8 nm along two shift axes; 5.7 -42.4 nm on specimen axes Total_Shift = 21.983132 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.02 microns changed by -2.62 to target drift = 0.08 nm/sec Measured defocus = -2.63 microns changed by 0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1868.tif 27 frames are being saved to U:\krios\20190717\PilA_1869.tif 27 frames are being saved to U:\krios\20190717\PilA_1870.tif 27 frames are being saved to U:\krios\20190717\PilA_1871.tif 27 frames are being saved to U:\krios\20190717\PilA_1872.tif 27 frames are being saved to U:\krios\20190717\PilA_1873.tif Running script 17 at item 381 (604.28, -440.56) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 80 20 60, reference by 23 151 130 20 Disparity in stage position after aligning to target position: -0.36 -0.42 (2nd move error 0.02 -0.27) Finished aligning to item in 1 rounds Trimming A by 24 85 20 60, reference by 24 80 20 60 -28.8 -0.5 unbinned pixels; 35.6 104.6 nm along two shift axes; 8.2 -112.1 nm on specimen axes Total_Shift = 57.677069 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.49 microns changed by -2.59 to target drift = 0.12 nm/sec Measured defocus = -1.12 microns changed by 0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1874.tif 27 frames are being saved to U:\krios\20190717\PilA_1875.tif 27 frames are being saved to U:\krios\20190717\PilA_1876.tif 27 frames are being saved to U:\krios\20190717\PilA_1877.tif 27 frames are being saved to U:\krios\20190717\PilA_1878.tif 27 frames are being saved to U:\krios\20190717\PilA_1879.tif Running script 17 at item 382 (601.64, -439.30) Remaining time to fill dewars is 29820 Dewars are NOT busy filling Trimming A by 21 170 20 250, reference by 286 22 20 290 Trimming A by 21 170 20 250, reference by 275 23 175 20 Trimming A by 21 170 20 250, reference by 22 165 20 250 Trimming A by 21 170 20 250, reference by 23 151 130 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.13 Trimming A by 21 70 20 90, reference by 22 165 20 250 Disparity in stage position after aligning to target position: -0.30 0.09 (2nd move error 0.01 0.20) Finished aligning to item in 2 rounds Trimming A by 21 65 20 90, reference by 21 70 20 90 -1.0 2.5 unbinned pixels; 10.4 0.2 nm along two shift axes; -9.4 -4.4 nm on specimen axes Total_Shift = 5.319474 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.52 microns changed by -3.72 to target drift = 0.15 nm/sec Measured defocus = -1.20 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1880.tif 27 frames are being saved to U:\krios\20190717\PilA_1881.tif 27 frames are being saved to U:\krios\20190717\PilA_1882.tif 27 frames are being saved to U:\krios\20190717\PilA_1883.tif 27 frames are being saved to U:\krios\20190717\PilA_1884.tif 27 frames are being saved to U:\krios\20190717\PilA_1885.tif Running script 17 at item 383 (586.72, -349.40) Remaining time to fill dewars is 29580 Dewars are NOT busy filling Trimming A by 260 22 230 20, reference by 240 22 20 230 Trimming A by 260 22 230 20, reference by 239 20 210 20 Trimming A by 260 22 230 20, reference by 23 225 20 230 Trimming A by 260 22 230 20, reference by 21 229 200 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.18 Trimming A by 125 22 180 20, reference by 239 20 210 20 Disparity in stage position after aligning to target position: -0.26 -0.25 (2nd move error 0.08 -0.09) Finished aligning to item in 2 rounds Trimming A by 125 22 185 20, reference by 125 22 180 20 -11.6 -1.5 unbinned pixels; 9.7 43.8 nm along two shift axes; 8.2 -44.6 nm on specimen axes Total_Shift = 23.291774 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.31 microns changed by -2.61 to target drift = 0.11 nm/sec Measured defocus = -1.24 microns changed by -0.06 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1886.tif 27 frames are being saved to U:\krios\20190717\PilA_1887.tif 27 frames are being saved to U:\krios\20190717\PilA_1888.tif 27 frames are being saved to U:\krios\20190717\PilA_1889.tif 27 frames are being saved to U:\krios\20190717\PilA_1890.tif 27 frames are being saved to U:\krios\20190717\PilA_1891.tif Running script 17 at item 384 (584.14, -347.75) Remaining time to fill dewars is 29520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 21 135 20, reference by 239 20 210 20 Disparity in stage position after aligning to target position: -0.26 -0.34 (2nd move error 0.08 -0.18) Finished aligning to item in 1 rounds Trimming A by 150 21 135 20, reference by 150 21 135 20 -19.9 -2.3 unbinned pixels; 17.5 75.3 nm along two shift axes; 13.3 -77.1 nm on specimen axes Total_Shift = 40.14733 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.41 microns changed by -3.81 to target drift = 0.11 nm/sec Measured defocus = -1.40 microns changed by -0.00 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1892.tif 27 frames are being saved to U:\krios\20190717\PilA_1893.tif 27 frames are being saved to U:\krios\20190717\PilA_1894.tif 27 frames are being saved to U:\krios\20190717\PilA_1895.tif 27 frames are being saved to U:\krios\20190717\PilA_1896.tif 27 frames are being saved to U:\krios\20190717\PilA_1897.tif Running script 17 at item 385 (581.66, -346.03) Remaining time to fill dewars is 29280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 175 21 105 30, reference by 239 20 210 20 Disparity in stage position after aligning to target position: -0.25 -0.39 (2nd move error 0.10 -0.23) Finished aligning to item in 1 rounds Trimming A by 170 20 105 30, reference by 175 21 105 30 -26.8 0.3 unbinned pixels; 36.2 96.0 nm along two shift axes; 4.4 -104.3 nm on specimen axes Total_Shift = 53.594634 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.85 microns changed by -2.35 to target drift = 0.12 nm/sec Measured defocus = -1.50 microns changed by -0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1898.tif 27 frames are being saved to U:\krios\20190717\PilA_1899.tif 27 frames are being saved to U:\krios\20190717\PilA_1900.tif 27 frames are being saved to U:\krios\20190717\PilA_1901.tif 27 frames are being saved to U:\krios\20190717\PilA_1902.tif 27 frames are being saved to U:\krios\20190717\PilA_1903.tif Running script 17 at item 386 (579.09, -344.92) Remaining time to fill dewars is 29160 Dewars are NOT busy filling Trimming A by 250 20 20 235, reference by 240 22 20 230 Trimming A by 250 20 20 235, reference by 239 20 210 20 Trimming A by 250 20 20 235, reference by 23 225 20 230 Trimming A by 250 20 20 235, reference by 21 229 200 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.12 Trimming A by 210 20 30 115, reference by 240 22 20 230 Disparity in stage position after aligning to target position: -0.07 0.12 (2nd move error 0.22 0.21) Finished aligning to item in 2 rounds Trimming A by 210 20 45 115, reference by 210 20 30 115 4.4 1.7 unbinned pixels; 0.5 18.3 nm along two shift axes; -7.5 16.7 nm on specimen axes Total_Shift = 9.366093 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.91 microns changed by -3.51 to target drift = 0.19 nm/sec Measured defocus = -1.59 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1904.tif 27 frames are being saved to U:\krios\20190717\PilA_1905.tif 27 frames are being saved to U:\krios\20190717\PilA_1906.tif 27 frames are being saved to U:\krios\20190717\PilA_1907.tif 27 frames are being saved to U:\krios\20190717\PilA_1908.tif 27 frames are being saved to U:\krios\20190717\PilA_1909.tif Running script 17 at item 387 (577.46, -347.39) Remaining time to fill dewars is 29100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 160 22 20 145, reference by 240 22 20 230 Disparity in stage position after aligning to target position: -0.15 -0.05 (2nd move error 0.13 0.05) Finished aligning to item in 1 rounds Trimming A by 165 21 70 135, reference by 160 22 20 145 0.4 -6.4 unbinned pixels; 24.0 8.2 nm along two shift axes; 24.8 2.8 nm on specimen axes Total_Shift = 12.791745 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.48 microns changed by -2.18 to target drift = 0.13 nm/sec Measured defocus = -1.71 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1910.tif 27 frames are being saved to U:\krios\20190717\PilA_1911.tif 27 frames are being saved to U:\krios\20190717\PilA_1912.tif 27 frames are being saved to U:\krios\20190717\PilA_1913.tif 27 frames are being saved to U:\krios\20190717\PilA_1914.tif 27 frames are being saved to U:\krios\20190717\PilA_1915.tif Running script 17 at item 388 (579.98, -349.25) Remaining time to fill dewars is 28860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 20 25 75, reference by 240 22 20 230 Disparity in stage position after aligning to target position: -0.04 0.07 (2nd move error 0.25 0.17) Finished aligning to item in 1 rounds Trimming A by 95 20 30 65, reference by 90 20 25 75 6.9 0.9 unbinned pixels; 5.9 26.3 nm along two shift axes; -4.8 26.8 nm on specimen axes Total_Shift = 13.993547 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.78 microns changed by -3.58 to target drift = 0.16 nm/sec Measured defocus = -1.76 microns changed by -0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1916.tif 27 frames are being saved to U:\krios\20190717\PilA_1917.tif 27 frames are being saved to U:\krios\20190717\PilA_1918.tif 27 frames are being saved to U:\krios\20190717\PilA_1919.tif 27 frames are being saved to U:\krios\20190717\PilA_1920.tif 27 frames are being saved to U:\krios\20190717\PilA_1921.tif Running script 17 at item 389 (582.60, -350.21) Remaining time to fill dewars is 28800 Dewars are NOT busy filling Trimming A by 260 20 225 20, reference by 240 22 20 230 Trimming A by 260 20 225 20, reference by 239 20 210 20 Trimming A by 260 20 225 20, reference by 23 225 20 230 Trimming A by 260 20 225 20, reference by 21 229 200 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.13 Trimming A by 75 20 80 20, reference by 239 20 210 20 Disparity in stage position after aligning to target position: -0.22 -0.35 (2nd move error 0.08 -0.25) Finished aligning to item in 2 rounds Trimming A by 75 20 75 20, reference by 75 20 80 20 -26.9 -2.6 unbinned pixels; 25.7 100.7 nm along two shift axes; 15.7 -104.2 nm on specimen axes Total_Shift = 54.068676 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.49 microns changed by -2.39 to target drift = 0.18 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1922.tif 27 frames are being saved to U:\krios\20190717\PilA_1923.tif 27 frames are being saved to U:\krios\20190717\PilA_1924.tif 27 frames are being saved to U:\krios\20190717\PilA_1925.tif 27 frames are being saved to U:\krios\20190717\PilA_1926.tif 27 frames are being saved to U:\krios\20190717\PilA_1927.tif Running script 17 at item 390 (585.21, -351.92) Remaining time to fill dewars is 28680 Dewars are NOT busy filling Trimming A by 21 235 215 20, reference by 240 22 20 230 Trimming A by 21 235 215 20, reference by 239 20 210 20 Trimming A by 21 235 215 20, reference by 23 225 20 230 Trimming A by 21 235 215 20, reference by 21 229 200 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.16 Trimming A by 41 61 135 20, reference by 21 229 200 20 Disparity in stage position after aligning to target position: -0.38 -0.37 (2nd move error -0.09 -0.24) Finished aligning to item in 2 rounds Trimming A by 35 65 135 20, reference by 41 61 135 20 -22.5 19.2 unbinned pixels; 99.9 52.1 nm along two shift axes; -69.9 -91.5 nm on specimen axes Total_Shift = 59.100166 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.89 microns changed by -3.89 to target drift = 0.01 nm/sec Measured defocus = -2.07 microns changed by 0.07 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1928.tif 27 frames are being saved to U:\krios\20190717\PilA_1929.tif 27 frames are being saved to U:\krios\20190717\PilA_1930.tif 27 frames are being saved to U:\krios\20190717\PilA_1931.tif 27 frames are being saved to U:\krios\20190717\PilA_1932.tif 27 frames are being saved to U:\krios\20190717\PilA_1933.tif Running script 17 at item 391 (583.57, -354.53) Remaining time to fill dewars is 28560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 110 95 20, reference by 21 229 200 20 Disparity in stage position after aligning to target position: -0.26 -0.44 (2nd move error 0.02 -0.30) Finished aligning to item in 1 rounds Trimming A by 23 115 95 20, reference by 23 110 95 20 -28.6 -1.7 unbinned pixels; 31.1 105.6 nm along two shift axes; 12.7 -111.0 nm on specimen axes Total_Shift = 57.370543 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.43 microns changed by -2.53 to target drift = 0.15 nm/sec Measured defocus = -2.16 microns changed by 0.06 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1934.tif 27 frames are being saved to U:\krios\20190717\PilA_1935.tif 27 frames are being saved to U:\krios\20190717\PilA_1936.tif 27 frames are being saved to U:\krios\20190717\PilA_1937.tif 27 frames are being saved to U:\krios\20190717\PilA_1938.tif 27 frames are being saved to U:\krios\20190717\PilA_1939.tif Running script 17 at item 392 (581.04, -352.75) Remaining time to fill dewars is 28380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 55 40 45, reference by 21 229 200 20 Disparity in stage position after aligning to target position: -0.26 -0.56 (2nd move error 0.03 -0.43) Finished aligning to item in 1 rounds Trimming A by 20 60 20 55, reference by 21 55 40 45 -31.5 -3.0 unbinned pixels; 30.3 117.9 nm along two shift axes; 18.1 -122.0 nm on specimen axes Total_Shift = 63.316152 Trimming A by 21 65 45 50, reference by 21 55 40 45 -0.1 -2.9 unbinned pixels; 10.5 4.8 nm along two shift axes; 11.3 0.1 nm on specimen axes Total_Shift = 5.787477 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.47 microns changed by -3.67 to target drift = 0.15 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1940.tif 27 frames are being saved to U:\krios\20190717\PilA_1941.tif 27 frames are being saved to U:\krios\20190717\PilA_1942.tif 27 frames are being saved to U:\krios\20190717\PilA_1943.tif 27 frames are being saved to U:\krios\20190717\PilA_1944.tif 27 frames are being saved to U:\krios\20190717\PilA_1945.tif Running script 17 at item 393 (578.25, -351.59) Remaining time to fill dewars is 28260 Dewars are NOT busy filling Trimming A by 255 20 55 230, reference by 240 22 20 230 Trimming A by 255 20 55 230, reference by 239 20 210 20 Trimming A by 255 20 55 230, reference by 23 225 20 230 Trimming A by 255 20 55 230, reference by 21 229 200 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.15 Trimming A by 42 32 20 110, reference by 240 22 20 230 Disparity in stage position after aligning to target position: 0.07 0.03 (2nd move error 0.33 0.15) Finished aligning to item in 2 rounds Trimming A by 46 25 20 100, reference by 42 32 20 110 2.5 -2.9 unbinned pixels; 13.8 4.8 nm along two shift axes; 10.6 10.4 nm on specimen axes Total_Shift = 7.638609 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.15 microns changed by -2.15 to target drift = 0.14 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1946.tif 27 frames are being saved to U:\krios\20190717\PilA_1947.tif 27 frames are being saved to U:\krios\20190717\PilA_1948.tif 27 frames are being saved to U:\krios\20190717\PilA_1949.tif 27 frames are being saved to U:\krios\20190717\PilA_1950.tif 27 frames are being saved to U:\krios\20190717\PilA_1951.tif Running script 17 at item 394 (575.80, -350.01) Remaining time to fill dewars is 28020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 23 20 175, reference by 240 22 20 230 Disparity in stage position after aligning to target position: 0.03 -0.14 (2nd move error 0.28 -0.01) Finished aligning to item in 1 rounds Trimming A by 97 21 20 180, reference by 115 23 20 175 0.2 -10.6 unbinned pixels; 39.1 15.0 nm along two shift axes; 41.0 3.2 nm on specimen axes Total_Shift = 21.116634 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.00 microns changed by -3.40 to target drift = 0.15 nm/sec Measured defocus = -2.44 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1952.tif 27 frames are being saved to U:\krios\20190717\PilA_1953.tif 27 frames are being saved to U:\krios\20190717\PilA_1954.tif 27 frames are being saved to U:\krios\20190717\PilA_1955.tif 27 frames are being saved to U:\krios\20190717\PilA_1956.tif 27 frames are being saved to U:\krios\20190717\PilA_1957.tif Running script 17 at item 395 (576.96, -354.20) Remaining time to fill dewars is 27960 Dewars are NOT busy filling Trimming A by 24 230 20 235, reference by 240 22 20 230 Trimming A by 24 230 20 235, reference by 239 20 210 20 Trimming A by 24 230 20 235, reference by 23 225 20 230 Trimming A by 24 230 20 235, reference by 21 229 200 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.19 Trimming A by 20 115 20 145, reference by 23 225 20 230 Disparity in stage position after aligning to target position: -0.26 -0.07 (2nd move error -0.11 0.10) Finished aligning to item in 2 rounds Trimming A by 21 110 20 145, reference by 20 115 20 145 -1.1 22.9 unbinned pixels; 85.5 30.5 nm along two shift axes; -88.7 -9.1 nm on specimen axes Total_Shift = 45.798063 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.31 microns changed by -2.19 to target drift = 0.13 nm/sec Measured defocus = -2.51 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1958.tif 27 frames are being saved to U:\krios\20190717\PilA_1959.tif 27 frames are being saved to U:\krios\20190717\PilA_1960.tif 27 frames are being saved to U:\krios\20190717\PilA_1961.tif 27 frames are being saved to U:\krios\20190717\PilA_1962.tif 27 frames are being saved to U:\krios\20190717\PilA_1963.tif Running script 17 at item 396 (579.51, -355.94) Remaining time to fill dewars is 27720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 140 20 120, reference by 23 225 20 230 Disparity in stage position after aligning to target position: -0.14 0.04 (2nd move error 0.01 0.21) Finished aligning to item in 1 rounds Trimming A by 21 140 70 105, reference by 22 140 20 120 2.5 0.8 unbinned pixels; 0.4 10.3 nm along two shift axes; -3.6 9.7 nm on specimen axes Total_Shift = 5.322135 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 1.00 microns changed by -3.60 to target drift = 0.13 nm/sec Measured defocus = -2.61 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1964.tif 27 frames are being saved to U:\krios\20190717\PilA_1965.tif 27 frames are being saved to U:\krios\20190717\PilA_1966.tif 27 frames are being saved to U:\krios\20190717\PilA_1967.tif 27 frames are being saved to U:\krios\20190717\PilA_1968.tif 27 frames are being saved to U:\krios\20190717\PilA_1969.tif Running script 17 at item 397 (581.96, -357.08) Remaining time to fill dewars is 27660 Dewars are NOT busy filling Trimming A by 21 235 215 20, reference by 240 22 20 230 Trimming A by 21 235 215 20, reference by 239 20 210 20 Trimming A by 21 235 215 20, reference by 23 225 20 230 Trimming A by 21 235 215 20, reference by 21 229 200 20 Disparity in stage position after aligning to center of map frame: -0.27 -0.14 Trimming A by 20 160 65 75, reference by 21 229 200 20 Disparity in stage position after aligning to target position: -0.21 -0.49 (2nd move error 0.06 -0.37) Finished aligning to item in 2 rounds Trimming A by 20 160 75 85, reference by 20 160 65 75 -30.0 -2.6 unbinned pixels; 29.5 111.9 nm along two shift axes; 16.6 -116.1 nm on specimen axes Total_Shift = 60.221535 Trimming A by 20 170 65 75, reference by 20 160 65 75 0.5 -1.3 unbinned pixels; 5.3 0.2 nm along two shift axes; 4.9 2.1 nm on specimen axes Total_Shift = 2.73582 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.40 microns changed by -2.50 to target drift = 0.13 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1970.tif 27 frames are being saved to U:\krios\20190717\PilA_1971.tif 27 frames are being saved to U:\krios\20190717\PilA_1972.tif 27 frames are being saved to U:\krios\20190717\PilA_1973.tif 27 frames are being saved to U:\krios\20190717\PilA_1974.tif 27 frames are being saved to U:\krios\20190717\PilA_1975.tif Running script 17 at item 398 (181.15, -190.75) Remaining time to fill dewars is 27420 Dewars are NOT busy filling Trimming A by 235 21 210 20, reference by 215 22 20 215 Trimming A by 235 21 210 20, reference by 220 20 180 20 Trimming A by 235 21 210 20, reference by 24 195 20 205 Trimming A by 235 21 210 20, reference by 22 190 180 20 Disparity in stage position after aligning to center of map frame: -0.45 -0.22 Trimming A by 130 20 155 20, reference by 220 20 180 20 Disparity in stage position after aligning to target position: -0.42 -0.25 (2nd move error 0.02 -0.05) Finished aligning to item in 2 rounds Trimming A by 130 20 155 20, reference by 130 20 155 20 -8.1 -1.0 unbinned pixels; 6.9 30.7 nm along two shift axes; 5.6 -31.3 nm on specimen axes Total_Shift = 16.327912 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.49 microns changed by -2.69 to target drift = 0.19 nm/sec Measured defocus = -1.24 microns changed by 0.04 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1976.tif 27 frames are being saved to U:\krios\20190717\PilA_1977.tif 27 frames are being saved to U:\krios\20190717\PilA_1978.tif 27 frames are being saved to U:\krios\20190717\PilA_1979.tif 27 frames are being saved to U:\krios\20190717\PilA_1980.tif 27 frames are being saved to U:\krios\20190717\PilA_1981.tif Running script 17 at item 399 (178.62, -189.06) Remaining time to fill dewars is 27300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 20 125 20, reference by 220 20 180 20 Disparity in stage position after aligning to target position: -0.36 -0.31 (2nd move error 0.08 -0.12) Finished aligning to item in 1 rounds Trimming A by 145 20 125 20, reference by 145 20 125 20 -14.4 -2.4 unbinned pixels; 9.8 55.4 nm along two shift axes; 12.5 -55.3 nm on specimen axes Total_Shift = 29.124762 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.32 microns changed by -2.62 to target drift = 0.11 nm/sec Measured defocus = -1.26 microns changed by -0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1982.tif 27 frames are being saved to U:\krios\20190717\PilA_1983.tif 27 frames are being saved to U:\krios\20190717\PilA_1984.tif 27 frames are being saved to U:\krios\20190717\PilA_1985.tif 27 frames are being saved to U:\krios\20190717\PilA_1986.tif 27 frames are being saved to U:\krios\20190717\PilA_1987.tif Running script 17 at item 400 (176.12, -187.34) Remaining time to fill dewars is 27120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 170 20 95 20, reference by 220 20 180 20 Disparity in stage position after aligning to target position: -0.42 -0.40 (2nd move error 0.02 -0.20) Finished aligning to item in 1 rounds Trimming A by 170 20 90 20, reference by 170 20 95 20 -25.3 -0.0 unbinned pixels; 32.9 91.0 nm along two shift axes; 5.4 -98.3 nm on specimen axes Total_Shift = 50.530037 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.22 microns changed by -2.62 to target drift = 0.11 nm/sec Measured defocus = -1.44 microns changed by 0.04 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1988.tif 27 frames are being saved to U:\krios\20190717\PilA_1989.tif 27 frames are being saved to U:\krios\20190717\PilA_1990.tif 27 frames are being saved to U:\krios\20190717\PilA_1991.tif 27 frames are being saved to U:\krios\20190717\PilA_1992.tif 27 frames are being saved to U:\krios\20190717\PilA_1993.tif Running script 17 at item 401 (173.64, -186.18) Remaining time to fill dewars is 27000 Dewars are NOT busy filling Trimming A by 220 22 20 215, reference by 215 22 20 215 Trimming A by 220 22 20 215, reference by 220 20 180 20 Trimming A by 220 22 20 215, reference by 24 195 20 205 Trimming A by 220 22 20 215, reference by 22 190 180 20 Disparity in stage position after aligning to center of map frame: -0.40 -0.13 Trimming A by 210 20 25 105, reference by 215 22 20 215 Disparity in stage position after aligning to target position: -0.37 0.07 (2nd move error 0.02 0.19) Finished aligning to item in 2 rounds Trimming A by 210 20 40 110, reference by 210 20 25 105 2.0 1.3 unbinned pixels; 2.0 9.1 nm along two shift axes; -5.3 7.5 nm on specimen axes Total_Shift = 4.721085 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.94 microns changed by -2.44 to target drift = 0.16 nm/sec Measured defocus = -1.47 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_1994.tif 27 frames are being saved to U:\krios\20190717\PilA_1995.tif 27 frames are being saved to U:\krios\20190717\PilA_1996.tif 27 frames are being saved to U:\krios\20190717\PilA_1997.tif 27 frames are being saved to U:\krios\20190717\PilA_1998.tif 27 frames are being saved to U:\krios\20190717\PilA_1999.tif Running script 17 at item 402 (171.98, -188.75) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 160 20 20 135, reference by 215 22 20 215 Disparity in stage position after aligning to target position: -0.35 -0.06 (2nd move error 0.04 0.06) Finished aligning to item in 1 rounds Trimming A by 160 20 20 135, reference by 160 20 20 135 -1.7 -0.2 unbinned pixels; 1.4 6.6 nm along two shift axes; 1.3 -6.7 nm on specimen axes Total_Shift = 3.501438 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.84 microns changed by -2.44 to target drift = 0.10 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2000.tif 27 frames are being saved to U:\krios\20190717\PilA_2001.tif 27 frames are being saved to U:\krios\20190717\PilA_2002.tif 27 frames are being saved to U:\krios\20190717\PilA_2003.tif 27 frames are being saved to U:\krios\20190717\PilA_2004.tif 27 frames are being saved to U:\krios\20190717\PilA_2005.tif Running script 17 at item 403 (174.48, -190.06) Remaining time to fill dewars is 26820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 100 20 20 70, reference by 215 22 20 215 Disparity in stage position after aligning to target position: -0.32 0.09 (2nd move error 0.07 0.20) Finished aligning to item in 1 rounds Trimming A by 105 23 20 70, reference by 100 20 20 70 -0.3 -0.9 unbinned pixels; 2.8 2.2 nm along two shift axes; 3.4 -0.8 nm on specimen axes Total_Shift = 1.796487 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.69 microns changed by -2.39 to target drift = 0.16 nm/sec Measured defocus = -1.63 microns changed by -0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2006.tif 27 frames are being saved to U:\krios\20190717\PilA_2007.tif 27 frames are being saved to U:\krios\20190717\PilA_2008.tif 27 frames are being saved to U:\krios\20190717\PilA_2009.tif 27 frames are being saved to U:\krios\20190717\PilA_2010.tif 27 frames are being saved to U:\krios\20190717\PilA_2011.tif Running script 17 at item 404 (177.05, -191.55) Remaining time to fill dewars is 26580 Dewars are NOT busy filling Trimming A by 235 21 205 20, reference by 215 22 20 215 Trimming A by 235 21 205 20, reference by 220 20 180 20 Trimming A by 235 21 205 20, reference by 24 195 20 205 Trimming A by 235 21 205 20, reference by 22 190 180 20 Disparity in stage position after aligning to center of map frame: -0.39 -0.15 Trimming A by 75 20 65 20, reference by 220 20 180 20 Disparity in stage position after aligning to target position: -0.37 -0.36 (2nd move error 0.02 -0.24) Finished aligning to item in 2 rounds Trimming A by 75 24 60 20, reference by 75 20 65 20 -28.0 0.4 unbinned pixels; 38.1 100.3 nm along two shift axes; 4.4 -109.1 nm on specimen axes Total_Shift = 56.06524 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.71 microns changed by -2.51 to target drift = 0.10 nm/sec Measured defocus = -1.84 microns changed by 0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2012.tif 27 frames are being saved to U:\krios\20190717\PilA_2013.tif 27 frames are being saved to U:\krios\20190717\PilA_2014.tif 27 frames are being saved to U:\krios\20190717\PilA_2015.tif 27 frames are being saved to U:\krios\20190717\PilA_2016.tif 27 frames are being saved to U:\krios\20190717\PilA_2017.tif Running script 17 at item 405 (179.50, -193.35) Remaining time to fill dewars is 26400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 50 27 90 20, reference by 220 20 180 20 Disparity in stage position after aligning to target position: -0.26 -0.23 (2nd move error 0.12 -0.11) Finished aligning to item in 1 rounds Trimming A by 42 23 100 20, reference by 50 27 90 20 -11.4 -0.6 unbinned pixels; 12.9 42.0 nm along two shift axes; 4.6 -44.4 nm on specimen axes Total_Shift = 22.893667 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.46 microns changed by -2.36 to target drift = 0.13 nm/sec Measured defocus = -1.91 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2018.tif 27 frames are being saved to U:\krios\20190717\PilA_2019.tif 27 frames are being saved to U:\krios\20190717\PilA_2020.tif 27 frames are being saved to U:\krios\20190717\PilA_2021.tif 27 frames are being saved to U:\krios\20190717\PilA_2022.tif 27 frames are being saved to U:\krios\20190717\PilA_2023.tif Running script 17 at item 407 (178.08, -195.77) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Trimming A by 23 186 215 20, reference by 215 22 20 215 Trimming A by 23 186 215 20, reference by 220 20 180 20 Trimming A by 23 186 215 20, reference by 24 195 20 205 Trimming A by 23 186 215 20, reference by 22 190 180 20 Disparity in stage position after aligning to center of map frame: -0.36 -0.18 Trimming A by 20 65 65 20, reference by 22 190 180 20 Disparity in stage position after aligning to target position: -0.48 -0.38 (2nd move error -0.12 -0.22) Finished aligning to item in 2 rounds Trimming A by 22 38 80 20, reference by 20 65 65 20 -24.5 24.7 unbinned pixels; 122.6 51.0 nm along two shift axes; -90.8 -100.4 nm on specimen axes Total_Shift = 69.457779 Trimming A by 21 37 80 20, reference by 20 65 65 20 1.0 15.6 unbinned pixels; 55.9 27.1 nm along two shift axes; -60.7 0.7 nm on specimen axes Total_Shift = 31.186206 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.43 microns changed by -2.43 to target drift = 0.03 nm/sec Measured defocus = -2.02 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2024.tif 27 frames are being saved to U:\krios\20190717\PilA_2025.tif 27 frames are being saved to U:\krios\20190717\PilA_2026.tif 27 frames are being saved to U:\krios\20190717\PilA_2027.tif 27 frames are being saved to U:\krios\20190717\PilA_2028.tif 27 frames are being saved to U:\krios\20190717\PilA_2029.tif Running script 17 at item 408 (175.56, -193.96) Remaining time to fill dewars is 26100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 22 190 180 20 Disparity in stage position after aligning to target position: -0.43 -0.57 (2nd move error -0.07 -0.41) Finished aligning to item in 1 rounds -29.8 13.3 unbinned pixels; 87.9 87.2 nm along two shift axes; -45.6 -118.7 nm on specimen axes Total_Shift = 65.28421 -0.7 7.7 unbinned pixels; 29.3 8.9 nm along two shift axes; -29.8 -4.5 nm on specimen axes Total_Shift = 15.493514 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.28 microns changed by -2.38 to target drift = 0.03 nm/sec Measured defocus = -2.11 microns changed by 0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2030.tif 27 frames are being saved to U:\krios\20190717\PilA_2031.tif 27 frames are being saved to U:\krios\20190717\PilA_2032.tif 27 frames are being saved to U:\krios\20190717\PilA_2033.tif 27 frames are being saved to U:\krios\20190717\PilA_2034.tif 27 frames are being saved to U:\krios\20190717\PilA_2035.tif Running script 17 at item 409 (172.88, -192.90) Remaining time to fill dewars is 25980 Dewars are NOT busy filling Trimming A by 225 20 20 215, reference by 215 22 20 215 Trimming A by 225 20 20 215, reference by 220 20 180 20 Trimming A by 225 20 20 215, reference by 24 195 20 205 Trimming A by 225 20 20 215, reference by 22 190 180 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.16 Trimming A by 51 20 20 90, reference by 215 22 20 215 Disparity in stage position after aligning to target position: -0.24 0.06 (2nd move error 0.13 0.20) Finished aligning to item in 2 rounds Trimming A by 52 22 20 90, reference by 51 20 20 90 1.9 1.6 unbinned pixels; 3.3 9.1 nm along two shift axes; -6.5 6.9 nm on specimen axes Total_Shift = 4.858964 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.10 microns changed by -2.30 to target drift = 0.13 nm/sec Measured defocus = -2.16 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2036.tif 27 frames are being saved to U:\krios\20190717\PilA_2037.tif 27 frames are being saved to U:\krios\20190717\PilA_2038.tif 27 frames are being saved to U:\krios\20190717\PilA_2039.tif 27 frames are being saved to U:\krios\20190717\PilA_2040.tif 27 frames are being saved to U:\krios\20190717\PilA_2041.tif Running script 17 at item 410 (170.41, -191.26) Remaining time to fill dewars is 25740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 112 20 20 165, reference by 215 22 20 215 Disparity in stage position after aligning to target position: -0.26 -0.09 (2nd move error 0.11 0.04) Finished aligning to item in 1 rounds Trimming A by 116 21 20 160, reference by 112 20 20 165 -0.8 -4.4 unbinned pixels; 15.1 9.5 nm along two shift axes; 17.3 -2.2 nm on specimen axes Total_Shift = 8.925856 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.03 microns changed by -2.33 to target drift = 0.14 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2042.tif 27 frames are being saved to U:\krios\20190717\PilA_2043.tif 27 frames are being saved to U:\krios\20190717\PilA_2044.tif 27 frames are being saved to U:\krios\20190717\PilA_2045.tif 27 frames are being saved to U:\krios\20190717\PilA_2046.tif 27 frames are being saved to U:\krios\20190717\PilA_2047.tif Running script 17 at item 411 (168.75, -193.61) Remaining time to fill dewars is 25680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 96 20 20 185, reference by 215 22 20 215 Disparity in stage position after aligning to target position: -0.20 -0.17 (2nd move error 0.17 -0.04) Finished aligning to item in 1 rounds Trimming A by 87 22 20 185, reference by 96 20 20 185 -3.1 0.1 unbinned pixels; 4.3 11.1 nm along two shift axes; 0.4 -12.1 nm on specimen axes Total_Shift = 6.197905 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.16 microns changed by -2.24 to target drift = 0.10 nm/sec Measured defocus = -2.38 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2048.tif 27 frames are being saved to U:\krios\20190717\PilA_2049.tif 27 frames are being saved to U:\krios\20190717\PilA_2050.tif 27 frames are being saved to U:\krios\20190717\PilA_2051.tif 27 frames are being saved to U:\krios\20190717\PilA_2052.tif 27 frames are being saved to U:\krios\20190717\PilA_2053.tif Running script 17 at item 412 (171.51, -195.50) Remaining time to fill dewars is 25440 Dewars are NOT busy filling Trimming A by 20 190 20 205, reference by 215 22 20 215 Trimming A by 20 190 20 205, reference by 220 20 180 20 Trimming A by 20 190 20 205, reference by 24 195 20 205 Trimming A by 20 190 20 205, reference by 22 190 180 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.17 Trimming A by 26 65 20 100, reference by 24 195 20 205 Disparity in stage position after aligning to target position: -0.37 -0.09 (2nd move error -0.08 0.05) Finished aligning to item in 2 rounds Trimming A by 26 65 20 100, reference by 26 65 20 100 -0.3 14.9 unbinned pixels; 55.3 21.6 nm along two shift axes; -58.1 -4.1 nm on specimen axes Total_Shift = 29.890001 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.23 microns changed by -2.27 to target drift = 0.14 nm/sec Measured defocus = -2.50 microns changed by 0.00 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2054.tif 27 frames are being saved to U:\krios\20190717\PilA_2055.tif 27 frames are being saved to U:\krios\20190717\PilA_2056.tif 27 frames are being saved to U:\krios\20190717\PilA_2057.tif 27 frames are being saved to U:\krios\20190717\PilA_2058.tif 27 frames are being saved to U:\krios\20190717\PilA_2059.tif Running script 17 at item 413 (173.98, -197.24) Remaining time to fill dewars is 25380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 20 75, reference by 24 195 20 205 Disparity in stage position after aligning to target position: -0.34 0.00 (2nd move error -0.04 0.15) Finished aligning to item in 1 rounds Trimming A by 20 75 20 75, reference by 20 80 20 75 -4.1 15.6 unbinned pixels; 62.6 8.6 nm along two shift axes; -59.7 -19.3 nm on specimen axes Total_Shift = 32.203127 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.34 microns changed by -2.26 to target drift = 0.07 nm/sec Measured defocus = -2.60 microns changed by 0.00 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2060.tif 27 frames are being saved to U:\krios\20190717\PilA_2061.tif 27 frames are being saved to U:\krios\20190717\PilA_2062.tif 27 frames are being saved to U:\krios\20190717\PilA_2063.tif 27 frames are being saved to U:\krios\20190717\PilA_2064.tif 27 frames are being saved to U:\krios\20190717\PilA_2065.tif Running script 17 at item 414 (176.40, -198.38) Remaining time to fill dewars is 25320 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 21 191 215 20, reference by 215 22 20 215 Trimming A by 21 191 215 20, reference by 220 20 180 20 Trimming A by 21 191 215 20, reference by 24 195 20 205 Trimming A by 21 191 215 20, reference by 22 190 180 20 Disparity in stage position after aligning to center of map frame: -0.36 -0.17 Trimming A by 20 90 45 50, reference by 22 190 180 20 Disparity in stage position after aligning to target position: -0.43 -0.48 (2nd move error -0.08 -0.34) Finished aligning to item in 2 rounds Trimming A by 20 90 60 60, reference by 20 90 45 50 -27.8 15.6 unbinned pixels; 93.9 76.7 nm along two shift axes; -55.0 -111.6 nm on specimen axes Total_Shift = 63.888543 Trimming A by 20 100 40 50, reference by 20 90 45 50 0.4 9.3 unbinned pixels; 33.7 15.4 nm along two shift axes; -36.3 -0.4 nm on specimen axes Total_Shift = 18.641542 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.31 microns changed by -2.41 to target drift = 0.02 nm/sec Measured defocus = -1.10 microns changed by 0.00 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2066.tif 27 frames are being saved to U:\krios\20190717\PilA_2067.tif 27 frames are being saved to U:\krios\20190717\PilA_2068.tif 27 frames are being saved to U:\krios\20190717\PilA_2069.tif 27 frames are being saved to U:\krios\20190717\PilA_2070.tif 27 frames are being saved to U:\krios\20190717\PilA_2071.tif Running script 17 at item 415 (-38.93, -208.69) Remaining time to fill dewars is 25020 Dewars are NOT busy filling Trimming A by 180 20 150 20, reference by 186 22 20 230 Trimming A by 180 20 150 20, reference by 165 23 130 20 Trimming A by 180 20 150 20, reference by 23 185 20 200 Trimming A by 180 20 150 20, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.39 -0.26 Trimming A by 150 21 155 20, reference by 165 23 130 20 Disparity in stage position after aligning to target position: -0.40 -0.15 (2nd move error -0.02 0.09) Finished aligning to item in 2 rounds Trimming A by 150 21 155 20, reference by 150 21 155 20 0.8 4.2 unbinned pixels; 14.6 9.1 nm along two shift axes; -16.6 2.1 nm on specimen axes Total_Shift = 8.597494 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.17 microns changed by -2.37 to target drift = 0.12 nm/sec Measured defocus = -1.15 microns changed by -0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2072.tif 27 frames are being saved to U:\krios\20190717\PilA_2073.tif 27 frames are being saved to U:\krios\20190717\PilA_2074.tif 27 frames are being saved to U:\krios\20190717\PilA_2075.tif 27 frames are being saved to U:\krios\20190717\PilA_2076.tif 27 frames are being saved to U:\krios\20190717\PilA_2077.tif Running script 17 at item 416 (-41.49, -206.99) Remaining time to fill dewars is 24900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 160 22 135 20, reference by 165 23 130 20 Disparity in stage position after aligning to target position: -0.35 -0.28 (2nd move error 0.03 -0.05) Finished aligning to item in 1 rounds Trimming A by 160 22 135 20, reference by 160 22 135 20 -7.7 -1.7 unbinned pixels; 3.8 30.2 nm along two shift axes; 8.2 -29.6 nm on specimen axes Total_Shift = 15.749749 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.14 microns changed by -2.44 to target drift = 0.09 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2078.tif 27 frames are being saved to U:\krios\20190717\PilA_2079.tif 27 frames are being saved to U:\krios\20190717\PilA_2080.tif 27 frames are being saved to U:\krios\20190717\PilA_2081.tif 27 frames are being saved to U:\krios\20190717\PilA_2082.tif 27 frames are being saved to U:\krios\20190717\PilA_2083.tif Running script 17 at item 417 (-44.01, -205.31) Remaining time to fill dewars is 24720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 175 22 120 20, reference by 165 23 130 20 Disparity in stage position after aligning to target position: -0.33 -0.35 (2nd move error 0.05 -0.12) Finished aligning to item in 1 rounds Trimming A by 175 22 120 20, reference by 175 22 120 20 -15.1 -0.2 unbinned pixels; 19.1 54.5 nm along two shift axes; 3.8 -58.6 nm on specimen axes Total_Shift = 30.136722 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.09 microns changed by -2.49 to target drift = 0.12 nm/sec Measured defocus = -1.46 microns changed by 0.06 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2084.tif 27 frames are being saved to U:\krios\20190717\PilA_2085.tif 27 frames are being saved to U:\krios\20190717\PilA_2086.tif 27 frames are being saved to U:\krios\20190717\PilA_2087.tif 27 frames are being saved to U:\krios\20190717\PilA_2088.tif 27 frames are being saved to U:\krios\20190717\PilA_2089.tif Running script 17 at item 418 (-48.14, -206.28) Remaining time to fill dewars is 24600 Dewars are NOT busy filling Trimming A by 200 20 20 225, reference by 186 22 20 230 Trimming A by 200 20 20 225, reference by 165 23 130 20 Trimming A by 200 20 20 225, reference by 23 185 20 200 Trimming A by 200 20 20 225, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.17 Trimming A by 150 24 20 110, reference by 186 22 20 230 Disparity in stage position after aligning to target position: -0.24 0.09 (2nd move error 0.10 0.25) Finished aligning to item in 2 rounds Trimming A by 155 24 20 110, reference by 150 24 20 110 -3.9 -0.0 unbinned pixels; 4.9 14.1 nm along two shift axes; 1.0 -15.2 nm on specimen axes Total_Shift = 7.798066 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.95 microns changed by -2.45 to target drift = 0.12 nm/sec Measured defocus = -1.48 microns changed by -0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2090.tif 27 frames are being saved to U:\krios\20190717\PilA_2091.tif 27 frames are being saved to U:\krios\20190717\PilA_2092.tif 27 frames are being saved to U:\krios\20190717\PilA_2093.tif 27 frames are being saved to U:\krios\20190717\PilA_2094.tif 27 frames are being saved to U:\krios\20190717\PilA_2095.tif Running script 17 at item 419 (-45.62, -207.85) Remaining time to fill dewars is 24360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 100 23 65 25, reference by 165 23 130 20 Disparity in stage position after aligning to target position: -0.42 -0.45 (2nd move error -0.05 -0.21) Finished aligning to item in 1 rounds Trimming A by 95 24 60 30, reference by 100 23 65 25 -22.8 10.8 unbinned pixels; 69.6 65.9 nm along two shift axes; -37.3 -91.1 nm on specimen axes Total_Shift = 50.527435 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.78 microns changed by -2.38 to target drift = 0.01 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2096.tif 27 frames are being saved to U:\krios\20190717\PilA_2097.tif 27 frames are being saved to U:\krios\20190717\PilA_2098.tif 27 frames are being saved to U:\krios\20190717\PilA_2099.tif 27 frames are being saved to U:\krios\20190717\PilA_2100.tif 27 frames are being saved to U:\krios\20190717\PilA_2101.tif Running script 17 at item 420 (-43.10, -209.54) Remaining time to fill dewars is 24300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 85 24 75 20, reference by 165 23 130 20 Disparity in stage position after aligning to target position: -0.44 -0.31 (2nd move error -0.06 -0.08) Finished aligning to item in 1 rounds Trimming A by 85 23 70 20, reference by 85 24 75 20 -9.1 16.0 unbinned pixels; 70.8 8.5 nm along two shift axes; -60.4 -38.7 nm on specimen axes Total_Shift = 36.826423 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.61 microns changed by -2.31 to target drift = 0.06 nm/sec Measured defocus = -1.70 microns changed by 0.00 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2102.tif 27 frames are being saved to U:\krios\20190717\PilA_2103.tif 27 frames are being saved to U:\krios\20190717\PilA_2104.tif 27 frames are being saved to U:\krios\20190717\PilA_2105.tif 27 frames are being saved to U:\krios\20190717\PilA_2106.tif 27 frames are being saved to U:\krios\20190717\PilA_2107.tif Running script 17 at item 421 (-40.61, -211.25) Remaining time to fill dewars is 24180 Dewars are NOT busy filling Trimming A by 180 20 150 20, reference by 186 22 20 230 Trimming A by 180 20 150 20, reference by 165 23 130 20 Trimming A by 180 20 150 20, reference by 23 185 20 200 Trimming A by 180 20 150 20, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.25 Trimming A by 62 22 100 20, reference by 165 23 130 20 Disparity in stage position after aligning to target position: -0.31 -0.27 (2nd move error 0.06 -0.04) Finished aligning to item in 2 rounds Trimming A by 80 24 85 20, reference by 62 22 100 20 -5.6 0.5 unbinned pixels; 9.0 19.3 nm along two shift axes; -0.6 -21.7 nm on specimen axes Total_Shift = 11.164178 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.40 microns changed by -2.20 to target drift = 0.11 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2108.tif 27 frames are being saved to U:\krios\20190717\PilA_2109.tif 27 frames are being saved to U:\krios\20190717\PilA_2110.tif 27 frames are being saved to U:\krios\20190717\PilA_2111.tif 27 frames are being saved to U:\krios\20190717\PilA_2112.tif 27 frames are being saved to U:\krios\20190717\PilA_2113.tif Running script 17 at item 422 (-38.10, -212.93) Remaining time to fill dewars is 23880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 60 110 20, reference by 165 23 130 20 Disparity in stage position after aligning to target position: -0.28 -0.10 (2nd move error 0.09 0.13) Finished aligning to item in 1 rounds Trimming A by 50 31 135 20, reference by 65 60 110 20 -0.3 1.3 unbinned pixels; 5.1 0.9 nm along two shift axes; -4.9 -1.4 nm on specimen axes Total_Shift = 2.623301 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.34 microns changed by -2.24 to target drift = 0.13 nm/sec Measured defocus = -1.93 microns changed by 0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2114.tif 27 frames are being saved to U:\krios\20190717\PilA_2115.tif 27 frames are being saved to U:\krios\20190717\PilA_2116.tif 27 frames are being saved to U:\krios\20190717\PilA_2117.tif 27 frames are being saved to U:\krios\20190717\PilA_2118.tif 27 frames are being saved to U:\krios\20190717\PilA_2119.tif Running script 17 at item 423 (-35.50, -214.65) Remaining time to fill dewars is 23760 Dewars are NOT busy filling Trimming A by 22 150 130 20, reference by 186 22 20 230 Trimming A by 22 150 130 20, reference by 165 23 130 20 Trimming A by 22 150 130 20, reference by 23 185 20 200 Trimming A by 22 150 130 20, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.29 Trimming A by 21 106 200 20, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.37 -0.04 (2nd move error -0.01 0.23) Finished aligning to item in 2 rounds Trimming A by 21 101 200 20, reference by 21 106 200 20 -5.6 4.1 unbinned pixels; 22.5 13.9 nm along two shift axes; -14.9 -22.6 nm on specimen axes Total_Shift = 13.909251 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.14 microns changed by -2.14 to target drift = 0.07 nm/sec Measured defocus = -1.95 microns changed by -0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2120.tif 27 frames are being saved to U:\krios\20190717\PilA_2121.tif 27 frames are being saved to U:\krios\20190717\PilA_2122.tif 27 frames are being saved to U:\krios\20190717\PilA_2123.tif 27 frames are being saved to U:\krios\20190717\PilA_2124.tif 27 frames are being saved to U:\krios\20190717\PilA_2125.tif Running script 17 at item 424 (-37.10, -217.23) Remaining time to fill dewars is 23580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 122 190 20, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.38 -0.17 (2nd move error -0.01 0.09) Finished aligning to item in 1 rounds Trimming A by 23 121 190 20, reference by 21 122 190 20 0.1 2.7 unbinned pixels; 9.7 4.3 nm along two shift axes; -10.4 -0.3 nm on specimen axes Total_Shift = 5.33231 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = -0.02 microns changed by -2.08 to target drift = 0.09 nm/sec Measured defocus = -2.06 microns changed by -0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2126.tif 27 frames are being saved to U:\krios\20190717\PilA_2127.tif 27 frames are being saved to U:\krios\20190717\PilA_2128.tif 27 frames are being saved to U:\krios\20190717\PilA_2129.tif 27 frames are being saved to U:\krios\20190717\PilA_2130.tif 27 frames are being saved to U:\krios\20190717\PilA_2131.tif Running script 17 at item 425 (-39.67, -215.47) Remaining time to fill dewars is 23460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 61 110 20, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.40 -0.32 (2nd move error -0.04 -0.05) Finished aligning to item in 1 rounds Trimming A by 23 61 110 20, reference by 23 61 110 20 -7.8 9.0 unbinned pixels; 43.2 14.6 nm along two shift axes; -33.2 -32.3 nm on specimen axes Total_Shift = 23.776867 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.02 microns changed by -2.18 to target drift = 0.03 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2132.tif 27 frames are being saved to U:\krios\20190717\PilA_2133.tif 27 frames are being saved to U:\krios\20190717\PilA_2134.tif 27 frames are being saved to U:\krios\20190717\PilA_2135.tif 27 frames are being saved to U:\krios\20190717\PilA_2136.tif 27 frames are being saved to U:\krios\20190717\PilA_2137.tif Running script 17 at item 426 (-42.14, -213.70) Remaining time to fill dewars is 23220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 25 25 20, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.44 -0.36 (2nd move error -0.07 -0.10) Finished aligning to item in 1 rounds Trimming A by 20 20 30 20, reference by 21 25 25 20 -14.1 27.2 unbinned pixels; 118.5 9.8 nm along two shift axes; -102.9 -60.6 nm on specimen axes Total_Shift = 61.30072 Trimming A by 21 25 25 20, reference by 21 25 25 20 -5.2 20.6 unbinned pixels; 82.4 12.4 nm along two shift axes; -79.0 -24.4 nm on specimen axes Total_Shift = 42.438076 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.28 microns changed by -2.02 to target drift = 1.18 nm/sec Measured defocus = -2.08 microns changed by -0.22 to target drift = 0.00 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2138.tif 27 frames are being saved to U:\krios\20190717\PilA_2139.tif 27 frames are being saved to U:\krios\20190717\PilA_2140.tif 27 frames are being saved to U:\krios\20190717\PilA_2141.tif 27 frames are being saved to U:\krios\20190717\PilA_2142.tif 27 frames are being saved to U:\krios\20190717\PilA_2143.tif Running script 17 at item 427 (-44.76, -212.07) Remaining time to fill dewars is 23160 Dewars are NOT busy filling Trimming A by 180 20 150 20, reference by 186 22 20 230 Trimming A by 180 20 150 20, reference by 165 23 130 20 Trimming A by 180 20 150 20, reference by 23 185 20 200 Trimming A by 180 20 150 20, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.32 -0.23 Trimming A by 20 20 20 20, reference by 165 23 130 20 Disparity in stage position after aligning to target position: -0.28 -0.44 (2nd move error 0.03 -0.23) Finished aligning to item in 2 rounds -28.3 -3.9 unbinned pixels; 22.7 107.6 nm along two shift axes; 21.0 -109.1 nm on specimen axes Total_Shift = 57.053451 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.13 microns changed by -2.27 to target drift = 0.18 nm/sec Measured defocus = -2.43 microns changed by 0.03 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2144.tif 27 frames are being saved to U:\krios\20190717\PilA_2145.tif 27 frames are being saved to U:\krios\20190717\PilA_2146.tif 27 frames are being saved to U:\krios\20190717\PilA_2147.tif 27 frames are being saved to U:\krios\20190717\PilA_2148.tif 27 frames are being saved to U:\krios\20190717\PilA_2149.tif Running script 17 at item 428 (-47.21, -210.38) Remaining time to fill dewars is 22920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 20 20 55, reference by 165 23 130 20 Disparity in stage position after aligning to target position: -0.31 -0.50 (2nd move error -0.00 -0.29) Finished aligning to item in 1 rounds Trimming A by 50 21 20 55, reference by 55 20 20 55 -26.9 4.0 unbinned pixels; 49.9 90.7 nm along two shift axes; -10.0 -105.4 nm on specimen axes Total_Shift = 54.350711 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.16 microns changed by -2.34 to target drift = 0.11 nm/sec Measured defocus = -2.53 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2150.tif 27 frames are being saved to U:\krios\20190717\PilA_2151.tif 27 frames are being saved to U:\krios\20190717\PilA_2152.tif 27 frames are being saved to U:\krios\20190717\PilA_2153.tif 27 frames are being saved to U:\krios\20190717\PilA_2154.tif 27 frames are being saved to U:\krios\20190717\PilA_2155.tif Running script 17 at item 429 (-49.79, -209.24) Remaining time to fill dewars is 22680 Dewars are NOT busy filling Trimming A by 200 20 20 225, reference by 186 22 20 230 Trimming A by 200 20 20 225, reference by 165 23 130 20 Trimming A by 200 20 20 225, reference by 23 185 20 200 Trimming A by 200 20 20 225, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.32 -0.20 Trimming A by 102 22 20 130, reference by 186 22 20 230 Disparity in stage position after aligning to target position: -0.23 -0.02 (2nd move error 0.08 0.16) Finished aligning to item in 2 rounds Trimming A by 120 21 20 115, reference by 102 22 20 130 -2.2 3.4 unbinned pixels; 15.3 2.7 nm along two shift axes; -12.7 -9.2 nm on specimen axes Total_Shift = 8.060253 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.32 microns changed by -2.27 to target drift = 0.13 nm/sec Measured defocus = -2.58 microns changed by -0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2156.tif 27 frames are being saved to U:\krios\20190717\PilA_2157.tif 27 frames are being saved to U:\krios\20190717\PilA_2158.tif 27 frames are being saved to U:\krios\20190717\PilA_2159.tif 27 frames are being saved to U:\krios\20190717\PilA_2160.tif 27 frames are being saved to U:\krios\20190717\PilA_2161.tif Running script 17 at item 430 (-51.46, -211.79) Remaining time to fill dewars is 22560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 66 20 20 155, reference by 186 22 20 230 Disparity in stage position after aligning to target position: -0.17 -0.18 (2nd move error 0.14 -0.01) Finished aligning to item in 1 rounds Trimming A by 65 22 20 150, reference by 66 20 20 155 -0.8 3.5 unbinned pixels; 14.1 2.3 nm along two shift axes; -13.6 -4.0 nm on specimen axes Total_Shift = 7.286769 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.21 microns changed by -2.31 to target drift = 0.08 nm/sec Measured defocus = -1.10 microns changed by 0.00 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2162.tif 27 frames are being saved to U:\krios\20190717\PilA_2163.tif 27 frames are being saved to U:\krios\20190717\PilA_2164.tif 27 frames are being saved to U:\krios\20190717\PilA_2165.tif 27 frames are being saved to U:\krios\20190717\PilA_2166.tif 27 frames are being saved to U:\krios\20190717\PilA_2167.tif Running script 17 at item 431 (-48.77, -213.46) Remaining time to fill dewars is 22380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 40 21 20 50, reference by 186 22 20 230 Disparity in stage position after aligning to target position: -0.08 -0.02 (2nd move error 0.24 0.16) Finished aligning to item in 1 rounds Trimming A by 35 21 20 50, reference by 40 21 20 50 2.5 -4.1 unbinned pixels; 18.4 2.8 nm along two shift axes; 15.5 10.6 nm on specimen axes Total_Shift = 9.643514 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.07 microns changed by -2.27 to target drift = 0.15 nm/sec Measured defocus = -1.17 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2168.tif 27 frames are being saved to U:\krios\20190717\PilA_2169.tif 27 frames are being saved to U:\krios\20190717\PilA_2170.tif 27 frames are being saved to U:\krios\20190717\PilA_2171.tif 27 frames are being saved to U:\krios\20190717\PilA_2172.tif 27 frames are being saved to U:\krios\20190717\PilA_2173.tif Running script 17 at item 432 (-46.28, -214.54) Remaining time to fill dewars is 22200 Dewars are NOT busy filling Trimming A by 22 155 130 20, reference by 186 22 20 230 Trimming A by 22 155 130 20, reference by 165 23 130 20 Trimming A by 22 155 130 20, reference by 23 185 20 200 Trimming A by 22 155 130 20, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.36 -0.22 Trimming A by 20 20 20 20, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.47 -0.53 (2nd move error -0.12 -0.33) Finished aligning to item in 2 rounds -28.6 32.3 unbinned pixels; 156.1 54.2 nm along two shift axes; -119.7 -118.0 nm on specimen axes Total_Shift = 86.286118 0.3 22.2 unbinned pixels; 81.3 34.4 nm along two shift axes; -86.4 -3.6 nm on specimen axes Total_Shift = 44.416236 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.98 microns changed by -2.28 to target drift = 0.02 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2174.tif 27 frames are being saved to U:\krios\20190717\PilA_2175.tif 27 frames are being saved to U:\krios\20190717\PilA_2176.tif 27 frames are being saved to U:\krios\20190717\PilA_2177.tif 27 frames are being saved to U:\krios\20190717\PilA_2178.tif 27 frames are being saved to U:\krios\20190717\PilA_2179.tif Running script 17 at item 433 (-43.72, -216.29) Remaining time to fill dewars is 21900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 25 20, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.34 -0.44 (2nd move error 0.01 -0.25) Finished aligning to item in 1 rounds Trimming A by 21 25 25 20, reference by 20 20 25 20 -26.5 0.7 unbinned pixels; 37.0 94.4 nm along two shift axes; 3.0 -103.2 nm on specimen axes Total_Shift = 53.002074 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.84 microns changed by -2.24 to target drift = 0.13 nm/sec Measured defocus = -1.42 microns changed by 0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2180.tif 27 frames are being saved to U:\krios\20190717\PilA_2181.tif 27 frames are being saved to U:\krios\20190717\PilA_2182.tif 27 frames are being saved to U:\krios\20190717\PilA_2183.tif 27 frames are being saved to U:\krios\20190717\PilA_2184.tif 27 frames are being saved to U:\krios\20190717\PilA_2185.tif Running script 17 at item 434 (-41.25, -218.06) Remaining time to fill dewars is 21780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 85 90 20, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.24 -0.31 (2nd move error 0.11 -0.12) Finished aligning to item in 1 rounds Trimming A by 24 81 100 20, reference by 20 85 90 20 -13.7 0.0 unbinned pixels; 18.0 49.3 nm along two shift axes; 2.8 -53.3 nm on specimen axes Total_Shift = 27.41442 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.67 microns changed by -2.17 to target drift = 0.13 nm/sec Measured defocus = -1.50 microns changed by 0.00 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2186.tif 27 frames are being saved to U:\krios\20190717\PilA_2187.tif 27 frames are being saved to U:\krios\20190717\PilA_2188.tif 27 frames are being saved to U:\krios\20190717\PilA_2189.tif 27 frames are being saved to U:\krios\20190717\PilA_2190.tif 27 frames are being saved to U:\krios\20190717\PilA_2191.tif Running script 17 at item 435 (-38.78, -219.75) Remaining time to fill dewars is 21540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 160 155 20, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.31 -0.22 (2nd move error 0.04 -0.03) Finished aligning to item in 1 rounds Trimming A by 21 155 160 20, reference by 23 160 155 20 -5.8 -1.7 unbinned pixels; 1.5 23.4 nm along two shift axes; 7.6 -22.3 nm on specimen axes Total_Shift = 12.080535 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.53 microns changed by -2.13 to target drift = 0.15 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2192.tif 27 frames are being saved to U:\krios\20190717\PilA_2193.tif 27 frames are being saved to U:\krios\20190717\PilA_2194.tif 27 frames are being saved to U:\krios\20190717\PilA_2195.tif 27 frames are being saved to U:\krios\20190717\PilA_2196.tif 27 frames are being saved to U:\krios\20190717\PilA_2197.tif Running script 17 at item 436 (-40.36, -222.36) Remaining time to fill dewars is 21360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 190 135 20, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.25 -0.27 (2nd move error 0.10 -0.07) Finished aligning to item in 1 rounds Trimming A by 20 190 140 20, reference by 23 190 135 20 -7.3 -0.2 unbinned pixels; 8.9 26.4 nm along two shift axes; 2.2 -28.3 nm on specimen axes Total_Shift = 14.557317 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.41 microns changed by -2.11 to target drift = 0.08 nm/sec Measured defocus = -1.74 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2198.tif 27 frames are being saved to U:\krios\20190717\PilA_2199.tif 27 frames are being saved to U:\krios\20190717\PilA_2200.tif 27 frames are being saved to U:\krios\20190717\PilA_2201.tif 27 frames are being saved to U:\krios\20190717\PilA_2202.tif 27 frames are being saved to U:\krios\20190717\PilA_2203.tif Running script 17 at item 437 (-42.92, -220.66) Remaining time to fill dewars is 21120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 115 75 30, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.32 -0.44 (2nd move error 0.03 -0.24) Finished aligning to item in 1 rounds Trimming A by 21 120 75 25, reference by 23 115 75 30 -27.0 -1.6 unbinned pixels; 29.3 99.7 nm along two shift axes; 12.0 -104.7 nm on specimen axes Total_Shift = 54.110945 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.38 microns changed by -2.18 to target drift = 0.16 nm/sec Measured defocus = -1.86 microns changed by 0.06 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2204.tif 27 frames are being saved to U:\krios\20190717\PilA_2205.tif 27 frames are being saved to U:\krios\20190717\PilA_2206.tif 27 frames are being saved to U:\krios\20190717\PilA_2207.tif 27 frames are being saved to U:\krios\20190717\PilA_2208.tif 27 frames are being saved to U:\krios\20190717\PilA_2209.tif Running script 17 at item 438 (-45.40, -218.90) Remaining time to fill dewars is 21000 Dewars are NOT busy filling Trimming A by 22 150 130 20, reference by 186 22 20 230 Trimming A by 22 150 130 20, reference by 165 23 130 20 Trimming A by 22 150 130 20, reference by 23 185 20 200 Trimming A by 22 150 130 20, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.33 -0.25 Trimming A by 24 80 20 70, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.33 -0.52 (2nd move error -0.02 -0.29) Finished aligning to item in 2 rounds Trimming A by 20 80 20 75, reference by 24 80 20 70 -27.6 5.9 unbinned pixels; 57.7 90.6 nm along two shift axes; -17.1 -108.8 nm on specimen axes Total_Shift = 56.520685 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.35 microns changed by -2.25 to target drift = 0.08 nm/sec Measured defocus = -1.99 microns changed by 0.09 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2210.tif 27 frames are being saved to U:\krios\20190717\PilA_2211.tif 27 frames are being saved to U:\krios\20190717\PilA_2212.tif 27 frames are being saved to U:\krios\20190717\PilA_2213.tif 27 frames are being saved to U:\krios\20190717\PilA_2214.tif 27 frames are being saved to U:\krios\20190717\PilA_2215.tif Running script 17 at item 439 (-47.89, -217.63) Remaining time to fill dewars is 20760 Dewars are NOT busy filling Trimming A by 23 185 20 195, reference by 186 22 20 230 Trimming A by 23 185 20 195, reference by 165 23 130 20 Trimming A by 23 185 20 195, reference by 23 185 20 200 Trimming A by 23 185 20 195, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.21 Trimming A by 20 75 20 80, reference by 23 185 20 200 Disparity in stage position after aligning to target position: -0.28 0.05 (2nd move error 0.06 0.23) Finished aligning to item in 2 rounds Trimming A by 20 70 20 75, reference by 20 75 20 80 1.3 2.3 unbinned pixels; 6.9 8.1 nm along two shift axes; -9.3 4.4 nm on specimen axes Total_Shift = 5.292883 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.11 microns changed by -2.11 to target drift = 0.14 nm/sec Measured defocus = -1.89 microns changed by -0.11 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2216.tif 27 frames are being saved to U:\krios\20190717\PilA_2217.tif 27 frames are being saved to U:\krios\20190717\PilA_2218.tif 27 frames are being saved to U:\krios\20190717\PilA_2219.tif 27 frames are being saved to U:\krios\20190717\PilA_2220.tif 27 frames are being saved to U:\krios\20190717\PilA_2221.tif Running script 17 at item 440 (-50.31, -215.99) Remaining time to fill dewars is 20580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 65 20 90, reference by 23 185 20 200 Disparity in stage position after aligning to target position: -0.32 -0.13 (2nd move error 0.01 0.06) Finished aligning to item in 1 rounds Trimming A by 21 65 20 90, reference by 21 65 20 90 -0.4 0.4 unbinned pixels; 2.0 0.7 nm along two shift axes; -1.5 -1.5 nm on specimen axes Total_Shift = 1.104708 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.05 microns changed by -2.15 to target drift = 0.12 nm/sec Measured defocus = -2.11 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2222.tif 27 frames are being saved to U:\krios\20190717\PilA_2223.tif 27 frames are being saved to U:\krios\20190717\PilA_2224.tif 27 frames are being saved to U:\krios\20190717\PilA_2225.tif 27 frames are being saved to U:\krios\20190717\PilA_2226.tif 27 frames are being saved to U:\krios\20190717\PilA_2227.tif Running script 17 at item 441 (-52.79, -214.32) Remaining time to fill dewars is 20340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 23 20 150, reference by 23 185 20 200 Disparity in stage position after aligning to target position: -0.40 -0.20 (2nd move error -0.06 -0.01) Finished aligning to item in 1 rounds Trimming A by 65 23 20 150, reference by 65 23 20 150 -0.5 11.9 unbinned pixels; 44.4 16.1 nm along two shift axes; -46.2 -4.5 nm on specimen axes Total_Shift = 23.837381 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.02 microns changed by -2.22 to target drift = 0.10 nm/sec Measured defocus = -2.17 microns changed by -0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2228.tif 27 frames are being saved to U:\krios\20190717\PilA_2229.tif 27 frames are being saved to U:\krios\20190717\PilA_2230.tif 27 frames are being saved to U:\krios\20190717\PilA_2231.tif 27 frames are being saved to U:\krios\20190717\PilA_2232.tif 27 frames are being saved to U:\krios\20190717\PilA_2233.tif Running script 17 at item 442 (-51.95, -218.60) Remaining time to fill dewars is 20100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 135 20 160, reference by 23 185 20 200 Disparity in stage position after aligning to target position: -0.26 -0.19 (2nd move error 0.08 0.00) Finished aligning to item in 1 rounds Trimming A by 20 130 20 160, reference by 24 135 20 160 -1.1 -0.5 unbinned pixels; 0.6 4.8 nm along two shift axes; 2.3 -4.2 nm on specimen axes Total_Shift = 2.448567 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.15 microns changed by -2.15 to target drift = 0.12 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2234.tif 27 frames are being saved to U:\krios\20190717\PilA_2235.tif 27 frames are being saved to U:\krios\20190717\PilA_2236.tif 27 frames are being saved to U:\krios\20190717\PilA_2237.tif 27 frames are being saved to U:\krios\20190717\PilA_2238.tif 27 frames are being saved to U:\krios\20190717\PilA_2239.tif Running script 17 at item 443 (-49.46, -220.35) Remaining time to fill dewars is 19920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 150 20 140, reference by 23 185 20 200 Disparity in stage position after aligning to target position: -0.30 -0.06 (2nd move error 0.04 0.13) Finished aligning to item in 1 rounds Trimming A by 21 145 20 140, reference by 20 150 20 140 1.9 0.2 unbinned pixels; 1.8 7.0 nm along two shift axes; -1.1 7.2 nm on specimen axes Total_Shift = 3.760665 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.35 microns changed by -2.05 to target drift = 0.11 nm/sec Measured defocus = -2.37 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2240.tif 27 frames are being saved to U:\krios\20190717\PilA_2241.tif 27 frames are being saved to U:\krios\20190717\PilA_2242.tif 27 frames are being saved to U:\krios\20190717\PilA_2243.tif 27 frames are being saved to U:\krios\20190717\PilA_2244.tif 27 frames are being saved to U:\krios\20190717\PilA_2245.tif Running script 17 at item 444 (-47.26, -221.60) Remaining time to fill dewars is 19800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 160 20 135, reference by 23 185 20 200 Disparity in stage position after aligning to target position: -0.21 0.05 (2nd move error 0.13 0.25) Finished aligning to item in 1 rounds Trimming A by 24 155 20 130, reference by 22 160 20 135 1.4 8.0 unbinned pixels; 27.4 17.1 nm along two shift axes; -31.3 3.9 nm on specimen axes Total_Shift = 16.182833 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.41 microns changed by -2.09 to target drift = 0.09 nm/sec Measured defocus = -2.55 microns changed by 0.05 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2246.tif 27 frames are being saved to U:\krios\20190717\PilA_2247.tif 27 frames are being saved to U:\krios\20190717\PilA_2248.tif 27 frames are being saved to U:\krios\20190717\PilA_2249.tif 27 frames are being saved to U:\krios\20190717\PilA_2250.tif 27 frames are being saved to U:\krios\20190717\PilA_2251.tif Running script 17 at item 445 (-44.50, -223.19) Remaining time to fill dewars is 19560 Dewars are NOT busy filling Trimming A by 22 150 130 20, reference by 186 22 20 230 Trimming A by 22 150 130 20, reference by 165 23 130 20 Trimming A by 22 150 130 20, reference by 23 185 20 200 Trimming A by 22 150 130 20, reference by 24 140 120 20 Disparity in stage position after aligning to center of map frame: -0.33 -0.22 Trimming A by 20 180 20 110, reference by 24 140 120 20 Disparity in stage position after aligning to target position: -0.17 -0.44 (2nd move error 0.15 -0.24) Finished aligning to item in 2 rounds Trimming A by 24 185 25 105, reference by 20 180 20 110 -21.5 -3.5 unbinned pixels; 15.2 82.8 nm along two shift axes; 18.2 -83.1 nm on specimen axes Total_Shift = 43.648421 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.60 microns changed by -2.00 to target drift = 0.18 nm/sec Measured defocus = -2.58 microns changed by -0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2252.tif 27 frames are being saved to U:\krios\20190717\PilA_2253.tif 27 frames are being saved to U:\krios\20190717\PilA_2254.tif 27 frames are being saved to U:\krios\20190717\PilA_2255.tif 27 frames are being saved to U:\krios\20190717\PilA_2256.tif 27 frames are being saved to U:\krios\20190717\PilA_2257.tif Running script 17 at item 446 (-395.36, -226.46) Remaining time to fill dewars is 19320 Dewars are NOT busy filling Trimming A by 20 150 145 20, reference by 220 23 20 205 Trimming A by 20 150 145 20, reference by 225 24 165 20 Trimming A by 20 150 145 20, reference by 23 145 20 160 Trimming A by 20 150 145 20, reference by 23 103 155 20 Disparity in stage position after aligning to center of map frame: -0.44 -0.25 Trimming A by 56 46 185 20, reference by 23 103 155 20 Disparity in stage position after aligning to target position: -0.40 -0.16 (2nd move error 0.02 0.06) Finished aligning to item in 2 rounds Trimming A by 60 46 185 20, reference by 56 46 185 20 -3.4 0.4 unbinned pixels; 6.1 11.7 nm along two shift axes; -1.0 -13.5 nm on specimen axes Total_Shift = 6.94727 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.43 microns changed by -3.53 to target drift = 0.23 nm/sec Measured defocus = -1.07 microns changed by -0.03 to target drift = 0.16 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2258.tif 27 frames are being saved to U:\krios\20190717\PilA_2259.tif 27 frames are being saved to U:\krios\20190717\PilA_2260.tif 27 frames are being saved to U:\krios\20190717\PilA_2261.tif 27 frames are being saved to U:\krios\20190717\PilA_2262.tif 27 frames are being saved to U:\krios\20190717\PilA_2263.tif Running script 17 at item 447 (-398.01, -224.80) Remaining time to fill dewars is 19260 Dewars are NOT busy filling Trimming A by 244 22 185 20, reference by 220 23 20 205 Trimming A by 244 22 185 20, reference by 225 24 165 20 Trimming A by 244 22 185 20, reference by 23 145 20 160 Trimming A by 244 22 185 20, reference by 23 103 155 20 Disparity in stage position after aligning to center of map frame: -0.41 -0.27 Trimming A by 100 23 115 20, reference by 225 24 165 20 Disparity in stage position after aligning to target position: -0.30 -0.30 (2nd move error 0.10 -0.05) Finished aligning to item in 2 rounds Trimming A by 100 24 120 20, reference by 100 23 115 20 -4.8 1.0 unbinned pixels; 9.8 15.8 nm along two shift axes; -2.8 -18.9 nm on specimen axes Total_Shift = 9.78236 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.40 microns changed by -3.60 to target drift = 0.07 nm/sec Measured defocus = -1.21 microns changed by 0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2264.tif 27 frames are being saved to U:\krios\20190717\PilA_2265.tif 27 frames are being saved to U:\krios\20190717\PilA_2266.tif 27 frames are being saved to U:\krios\20190717\PilA_2267.tif 27 frames are being saved to U:\krios\20190717\PilA_2268.tif 27 frames are being saved to U:\krios\20190717\PilA_2269.tif Running script 17 at item 448 (-400.48, -223.13) Remaining time to fill dewars is 18960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 101 22 110 20, reference by 225 24 165 20 Disparity in stage position after aligning to target position: -0.35 -0.31 (2nd move error 0.05 -0.06) Finished aligning to item in 1 rounds Trimming A by 115 20 100 20, reference by 101 22 110 20 -6.2 -1.1 unbinned pixels; 4.0 23.9 nm along two shift axes; 5.6 -23.8 nm on specimen axes Total_Shift = 12.560788 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.34 microns changed by -3.64 to target drift = 0.06 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2270.tif 27 frames are being saved to U:\krios\20190717\PilA_2271.tif 27 frames are being saved to U:\krios\20190717\PilA_2272.tif 27 frames are being saved to U:\krios\20190717\PilA_2273.tif 27 frames are being saved to U:\krios\20190717\PilA_2274.tif 27 frames are being saved to U:\krios\20190717\PilA_2275.tif Running script 17 at item 449 (-402.99, -221.43) Remaining time to fill dewars is 18780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 23 90 20, reference by 225 24 165 20 Disparity in stage position after aligning to target position: -0.35 -0.42 (2nd move error 0.04 -0.17) Finished aligning to item in 1 rounds Trimming A by 125 20 90 20, reference by 130 23 90 20 -20.6 -1.6 unbinned pixels; 21.1 76.4 nm along two shift axes; 10.5 -79.7 nm on specimen axes Total_Shift = 41.241513 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.07 microns changed by -2.46 to target drift = 0.10 nm/sec Measured defocus = -1.48 microns changed by 0.08 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2276.tif 27 frames are being saved to U:\krios\20190717\PilA_2277.tif 27 frames are being saved to U:\krios\20190717\PilA_2278.tif 27 frames are being saved to U:\krios\20190717\PilA_2279.tif 27 frames are being saved to U:\krios\20190717\PilA_2280.tif 27 frames are being saved to U:\krios\20190717\PilA_2281.tif Running script 17 at item 450 (-405.57, -220.25) Remaining time to fill dewars is 18540 Dewars are NOT busy filling Trimming A by 229 22 20 205, reference by 220 23 20 205 Trimming A by 229 22 20 205, reference by 225 24 165 20 Trimming A by 229 22 20 205, reference by 23 145 20 160 Trimming A by 229 22 20 205, reference by 23 103 155 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.17 Trimming A by 150 20 40 85, reference by 220 23 20 205 Disparity in stage position after aligning to target position: -0.18 0.05 (2nd move error 0.13 0.19) Finished aligning to item in 2 rounds Trimming A by 150 20 55 85, reference by 150 20 40 85 -0.7 -0.1 unbinned pixels; 0.5 2.8 nm along two shift axes; 0.7 -2.8 nm on specimen axes Total_Shift = 1.481887 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.13 microns changed by -3.63 to target drift = 0.17 nm/sec Measured defocus = -1.44 microns changed by -0.06 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2282.tif 27 frames are being saved to U:\krios\20190717\PilA_2283.tif 27 frames are being saved to U:\krios\20190717\PilA_2284.tif 27 frames are being saved to U:\krios\20190717\PilA_2285.tif 27 frames are being saved to U:\krios\20190717\PilA_2286.tif 27 frames are being saved to U:\krios\20190717\PilA_2287.tif Ending Navigator acquisition after current item Running script 17 at item 451 (-407.17, -222.84) Remaining time to fill dewars is 18300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 92 22 20 115, reference by 220 23 20 205 Disparity in stage position after aligning to target position: -0.21 -0.05 (2nd move error 0.10 0.10) Finished aligning to item in 1 rounds Trimming A by 110 23 20 95, reference by 92 22 20 115 1.1 -3.2 unbinned pixels; 13.1 0.9 nm along two shift axes; 12.1 4.8 nm on specimen axes Total_Shift = 6.698132 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.86 microns changed by -2.46 to target drift = 0.10 nm/sec Measured defocus = -1.64 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2288.tif 27 frames are being saved to U:\krios\20190717\PilA_2289.tif 27 frames are being saved to U:\krios\20190717\PilA_2290.tif 27 frames are being saved to U:\krios\20190717\PilA_2291.tif 27 frames are being saved to U:\krios\20190717\PilA_2292.tif 27 frames are being saved to U:\krios\20190717\PilA_2293.tif Running script 17 at item 452 (-404.71, -224.47) Remaining time to fill dewars is 18060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 36 20 20 40, reference by 220 23 20 205 Disparity in stage position after aligning to target position: -0.13 0.11 (2nd move error 0.17 0.25) Finished aligning to item in 1 rounds Trimming A by 40 20 25 35, reference by 36 20 20 40 0.5 5.5 unbinned pixels; 19.8 10.1 nm along two shift axes; -21.7 0.7 nm on specimen axes Total_Shift = 11.133566 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.94 microns changed by -3.64 to target drift = 0.13 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2294.tif 27 frames are being saved to U:\krios\20190717\PilA_2295.tif 27 frames are being saved to U:\krios\20190717\PilA_2296.tif 27 frames are being saved to U:\krios\20190717\PilA_2297.tif 27 frames are being saved to U:\krios\20190717\PilA_2298.tif 27 frames are being saved to U:\krios\20190717\PilA_2299.tif Running script 17 at item 453 (-402.10, -225.61) Remaining time to fill dewars is 17820 Dewars are NOT busy filling Trimming A by 240 22 170 20, reference by 220 23 20 205 Trimming A by 240 22 170 20, reference by 225 24 165 20 Trimming A by 240 22 170 20, reference by 23 145 20 160 Trimming A by 240 22 170 20, reference by 23 103 155 20 Disparity in stage position after aligning to center of map frame: -0.34 -0.22 Trimming A by 35 21 30 20, reference by 225 24 165 20 Disparity in stage position after aligning to target position: -0.25 -0.36 (2nd move error 0.08 -0.16) Finished aligning to item in 2 rounds Trimming A by 35 21 30 20, reference by 35 21 30 20 -18.8 -2.8 unbinned pixels; 14.1 71.8 nm along two shift axes; 14.9 -72.4 nm on specimen axes Total_Shift = 37.9445 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.58 microns changed by -2.38 to target drift = 0.15 nm/sec Measured defocus = -1.84 microns changed by 0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2300.tif 27 frames are being saved to U:\krios\20190717\PilA_2301.tif 27 frames are being saved to U:\krios\20190717\PilA_2302.tif 27 frames are being saved to U:\krios\20190717\PilA_2303.tif 27 frames are being saved to U:\krios\20190717\PilA_2304.tif 27 frames are being saved to U:\krios\20190717\PilA_2305.tif Running script 17 at item 454 (-399.53, -227.29) Remaining time to fill dewars is 17640 Dewars are NOT busy filling Trimming A by 20 140 135 20, reference by 220 23 20 205 Trimming A by 20 140 135 20, reference by 225 24 165 20 Trimming A by 20 140 135 20, reference by 23 145 20 160 Trimming A by 20 140 135 20, reference by 23 103 155 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.24 Trimming A by 22 25 50 20, reference by 23 103 155 20 Disparity in stage position after aligning to target position: -0.30 -0.38 (2nd move error 0.01 -0.16) Finished aligning to item in 2 rounds Trimming A by 20 20 55 20, reference by 22 25 50 20 -19.7 1.5 unbinned pixels; 31.3 68.7 nm along two shift axes; -1.8 -77.0 nm on specimen axes Total_Shift = 39.532934 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.72 microns changed by -3.62 to target drift = 0.06 nm/sec Measured defocus = -1.95 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2306.tif 27 frames are being saved to U:\krios\20190717\PilA_2307.tif 27 frames are being saved to U:\krios\20190717\PilA_2308.tif 27 frames are being saved to U:\krios\20190717\PilA_2309.tif 27 frames are being saved to U:\krios\20190717\PilA_2310.tif 27 frames are being saved to U:\krios\20190717\PilA_2311.tif Running script 17 at item 455 (-397.02, -229.05) Remaining time to fill dewars is 17460 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 24 55 140 20, reference by 23 103 155 20 Disparity in stage position after aligning to target position: -0.28 -0.28 (2nd move error 0.02 -0.06) Finished aligning to item in 1 rounds Trimming A by 24 55 140 20, reference by 24 55 140 20 -8.2 -1.0 unbinned pixels; 6.9 31.0 nm along two shift axes; 5.7 -31.6 nm on specimen axes Total_Shift = 16.478607 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.17 microns changed by -2.17 to target drift = 0.10 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2312.tif 27 frames are being saved to U:\krios\20190717\PilA_2313.tif 27 frames are being saved to U:\krios\20190717\PilA_2314.tif 27 frames are being saved to U:\krios\20190717\PilA_2315.tif 27 frames are being saved to U:\krios\20190717\PilA_2316.tif 27 frames are being saved to U:\krios\20190717\PilA_2317.tif Running script 17 at item 456 (-398.69, -231.65) Remaining time to fill dewars is 17280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 91 105 20, reference by 23 103 155 20 Disparity in stage position after aligning to target position: -0.21 -0.34 (2nd move error 0.10 -0.12) Finished aligning to item in 1 rounds Trimming A by 23 96 105 20, reference by 23 91 105 20 -12.0 -3.0 unbinned pixels; 4.7 47.8 nm along two shift axes; 14.2 -46.1 nm on specimen axes Total_Shift = 24.770286 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.48 microns changed by -3.58 to target drift = 0.11 nm/sec Measured defocus = -2.14 microns changed by 0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2318.tif 27 frames are being saved to U:\krios\20190717\PilA_2319.tif 27 frames are being saved to U:\krios\20190717\PilA_2320.tif 27 frames are being saved to U:\krios\20190717\PilA_2321.tif 27 frames are being saved to U:\krios\20190717\PilA_2322.tif 27 frames are being saved to U:\krios\20190717\PilA_2323.tif Running script 17 at item 457 (-401.17, -229.90) Remaining time to fill dewars is 17040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 40 25 20, reference by 23 103 155 20 Disparity in stage position after aligning to target position: -0.25 -0.44 (2nd move error 0.06 -0.22) Finished aligning to item in 1 rounds Trimming A by 20 40 30 20, reference by 20 40 25 20 -23.9 -0.7 unbinned pixels; 28.5 87.2 nm along two shift axes; 7.9 -92.9 nm on specimen axes Total_Shift = 47.842002 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.09 microns changed by -2.29 to target drift = 0.15 nm/sec Measured defocus = -2.21 microns changed by 0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2324.tif 27 frames are being saved to U:\krios\20190717\PilA_2325.tif 27 frames are being saved to U:\krios\20190717\PilA_2326.tif 27 frames are being saved to U:\krios\20190717\PilA_2327.tif 27 frames are being saved to U:\krios\20190717\PilA_2328.tif 27 frames are being saved to U:\krios\20190717\PilA_2329.tif Running script 17 at item 458 (-403.69, -228.13) Remaining time to fill dewars is 16920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 103 155 20 Disparity in stage position after aligning to target position: -0.33 -0.57 (2nd move error -0.02 -0.35) Finished aligning to item in 1 rounds -31.0 6.5 unbinned pixels; 64.5 101.6 nm along two shift axes; -18.9 -121.8 nm on specimen axes Total_Shift = 63.279714 -0.5 3.2 unbinned pixels; 12.4 2.8 nm along two shift axes; -12.2 -2.8 nm on specimen axes Total_Shift = 6.437311 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.47 microns changed by -3.77 to target drift = 0.05 nm/sec Measured defocus = -2.41 microns changed by 0.11 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2330.tif 27 frames are being saved to U:\krios\20190717\PilA_2331.tif 27 frames are being saved to U:\krios\20190717\PilA_2332.tif 27 frames are being saved to U:\krios\20190717\PilA_2333.tif 27 frames are being saved to U:\krios\20190717\PilA_2334.tif 27 frames are being saved to U:\krios\20190717\PilA_2335.tif Running script 17 at item 459 (-406.12, -227.08) Remaining time to fill dewars is 16680 Dewars are NOT busy filling Trimming A by 21 135 20 160, reference by 220 23 20 205 Trimming A by 21 135 20 160, reference by 225 24 165 20 Trimming A by 21 135 20 160, reference by 23 145 20 160 Trimming A by 21 135 20 160, reference by 23 103 155 20 Disparity in stage position after aligning to center of map frame: -0.26 -0.23 Trimming A by 20 20 20 30, reference by 23 145 20 160 Disparity in stage position after aligning to target position: -0.25 0.02 (2nd move error 0.00 0.22) Finished aligning to item in 2 rounds Trimming A by 25 20 20 30, reference by 20 20 20 30 2.0 3.0 unbinned pixels; 8.5 11.7 nm along two shift axes; -12.2 7.1 nm on specimen axes Total_Shift = 7.241518 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.06 microns changed by -2.34 to target drift = 0.11 nm/sec Measured defocus = -2.40 microns changed by 0.00 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2336.tif 27 frames are being saved to U:\krios\20190717\PilA_2337.tif 27 frames are being saved to U:\krios\20190717\PilA_2338.tif 27 frames are being saved to U:\krios\20190717\PilA_2339.tif 27 frames are being saved to U:\krios\20190717\PilA_2340.tif 27 frames are being saved to U:\krios\20190717\PilA_2341.tif Running script 17 at item 460 (-408.83, -225.46) Remaining time to fill dewars is 16440 Dewars are NOT busy filling Trimming A by 225 23 20 200, reference by 220 23 20 205 Trimming A by 225 23 20 200, reference by 225 24 165 20 Trimming A by 225 23 20 200, reference by 23 145 20 160 Trimming A by 225 23 20 200, reference by 23 103 155 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.21 Trimming A by 55 22 20 130, reference by 220 23 20 205 Disparity in stage position after aligning to target position: -0.06 -0.09 (2nd move error 0.17 0.10) Finished aligning to item in 2 rounds Trimming A by 58 21 20 125, reference by 55 22 20 130 -3.4 -1.8 unbinned pixels; 2.0 15.0 nm along two shift axes; 7.6 -12.9 nm on specimen axes Total_Shift = 7.693662 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.28 microns changed by -3.78 to target drift = 0.12 nm/sec Measured defocus = -2.65 microns changed by 0.15 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2342.tif 27 frames are being saved to U:\krios\20190717\PilA_2343.tif 27 frames are being saved to U:\krios\20190717\PilA_2344.tif 27 frames are being saved to U:\krios\20190717\PilA_2345.tif 27 frames are being saved to U:\krios\20190717\PilA_2346.tif 27 frames are being saved to U:\krios\20190717\PilA_2347.tif Running script 17 at item 461 (-410.27, -227.92) Remaining time to fill dewars is 16320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 43 23 20 135, reference by 23 145 20 160 Disparity in stage position after aligning to target position: -0.32 -0.20 (2nd move error -0.06 0.00) Finished aligning to item in 1 rounds Trimming A by 51 23 20 130, reference by 43 23 20 135 1.1 12.6 unbinned pixels; 44.8 22.8 nm along two shift axes; -49.1 1.6 nm on specimen axes Total_Shift = 25.214711 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.18 microns changed by -2.42 to target drift = 0.10 nm/sec Measured defocus = -2.57 microns changed by -0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2348.tif 27 frames are being saved to U:\krios\20190717\PilA_2349.tif 27 frames are being saved to U:\krios\20190717\PilA_2350.tif 27 frames are being saved to U:\krios\20190717\PilA_2351.tif 27 frames are being saved to U:\krios\20190717\PilA_2352.tif 27 frames are being saved to U:\krios\20190717\PilA_2353.tif Running script 17 at item 462 (-407.71, -229.67) Remaining time to fill dewars is 16080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 45 20 75, reference by 23 145 20 160 Disparity in stage position after aligning to target position: -0.22 -0.13 (2nd move error 0.03 0.08) Finished aligning to item in 1 rounds Trimming A by 22 45 20 70, reference by 22 45 20 75 -3.7 0.9 unbinned pixels; 8.1 11.9 nm along two shift axes; -2.7 -14.4 nm on specimen axes Total_Shift = 7.542424 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.40 microns changed by -2.50 to target drift = 0.08 nm/sec Measured defocus = -1.15 microns changed by 0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2354.tif 27 frames are being saved to U:\krios\20190717\PilA_2355.tif 27 frames are being saved to U:\krios\20190717\PilA_2356.tif 27 frames are being saved to U:\krios\20190717\PilA_2357.tif 27 frames are being saved to U:\krios\20190717\PilA_2358.tif 27 frames are being saved to U:\krios\20190717\PilA_2359.tif Running script 17 at item 463 (-405.24, -231.33) Remaining time to fill dewars is 15900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 50 20 50, reference by 23 145 20 160 Disparity in stage position after aligning to target position: -0.13 -0.02 (2nd move error 0.13 0.18) Finished aligning to item in 1 rounds Trimming A by 20 56 20 35, reference by 20 50 20 50 4.0 -1.6 unbinned pixels; 11.2 12.0 nm along two shift axes; 5.4 16.0 nm on specimen axes Total_Shift = 8.651478 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.48 microns changed by -3.68 to target drift = 0.14 nm/sec Measured defocus = -1.19 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2360.tif 27 frames are being saved to U:\krios\20190717\PilA_2361.tif 27 frames are being saved to U:\krios\20190717\PilA_2362.tif 27 frames are being saved to U:\krios\20190717\PilA_2363.tif 27 frames are being saved to U:\krios\20190717\PilA_2364.tif 27 frames are being saved to U:\krios\20190717\PilA_2365.tif Running script 17 at item 464 (-400.22, -234.23) Remaining time to fill dewars is 15600 Dewars are NOT busy filling Trimming A by 20 140 135 20, reference by 220 23 20 205 Trimming A by 20 140 135 20, reference by 225 24 165 20 Trimming A by 20 140 135 20, reference by 23 145 20 160 Trimming A by 20 140 135 20, reference by 23 103 155 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.21 Trimming A by 21 120 85 20, reference by 23 103 155 20 Disparity in stage position after aligning to target position: -0.23 -0.36 (2nd move error 0.04 -0.18) Finished aligning to item in 2 rounds Trimming A by 24 125 85 20, reference by 21 120 85 20 -21.8 -2.7 unbinned pixels; 18.6 82.3 nm along two shift axes; 14.9 -84.1 nm on specimen axes Total_Shift = 43.830165 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.40 microns changed by -3.70 to target drift = 0.10 nm/sec Measured defocus = -1.39 microns changed by 0.09 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2366.tif 27 frames are being saved to U:\krios\20190717\PilA_2367.tif 27 frames are being saved to U:\krios\20190717\PilA_2368.tif 27 frames are being saved to U:\krios\20190717\PilA_2369.tif 27 frames are being saved to U:\krios\20190717\PilA_2370.tif 27 frames are being saved to U:\krios\20190717\PilA_2371.tif Running script 17 at item 465 (-404.41, -235.49) Remaining time to fill dewars is 15420 Dewars are NOT busy filling Trimming A by 21 135 20 160, reference by 220 23 20 205 Trimming A by 21 135 20 160, reference by 225 24 165 20 Trimming A by 21 135 20 160, reference by 23 145 20 160 Trimming A by 21 135 20 160, reference by 23 103 155 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.25 Trimming A by 24 125 20 100, reference by 23 145 20 160 Disparity in stage position after aligning to target position: -0.11 0.05 (2nd move error 0.13 0.29) Finished aligning to item in 2 rounds Trimming A by 24 115 20 95, reference by 24 125 20 100 5.7 1.6 unbinned pixels; 1.3 22.8 nm along two shift axes; -7.6 21.7 nm on specimen axes Total_Shift = 11.780599 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.01 microns changed by -2.41 to target drift = 0.16 nm/sec Measured defocus = -1.40 microns changed by -0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2372.tif 27 frames are being saved to U:\krios\20190717\PilA_2373.tif 27 frames are being saved to U:\krios\20190717\PilA_2374.tif 27 frames are being saved to U:\krios\20190717\PilA_2375.tif 27 frames are being saved to U:\krios\20190717\PilA_2376.tif 27 frames are being saved to U:\krios\20190717\PilA_2377.tif Running script 17 at item 466 (-406.89, -233.96) Remaining time to fill dewars is 15300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 125 20 110, reference by 23 145 20 160 Disparity in stage position after aligning to target position: -0.15 -0.12 (2nd move error 0.09 0.11) Finished aligning to item in 1 rounds Trimming A by 20 110 20 115, reference by 22 125 20 110 -4.2 0.8 unbinned pixels; 8.4 14.0 nm along two shift axes; -2.2 -16.5 nm on specimen axes Total_Shift = 8.556874 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.23 microns changed by -3.73 to target drift = 0.07 nm/sec Measured defocus = -1.53 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2378.tif 27 frames are being saved to U:\krios\20190717\PilA_2379.tif 27 frames are being saved to U:\krios\20190717\PilA_2380.tif 27 frames are being saved to U:\krios\20190717\PilA_2381.tif 27 frames are being saved to U:\krios\20190717\PilA_2382.tif 27 frames are being saved to U:\krios\20190717\PilA_2383.tif Running script 17 at item 467 (-409.35, -232.19) Remaining time to fill dewars is 15060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 115 20 135, reference by 23 145 20 160 Disparity in stage position after aligning to target position: -0.19 -0.21 (2nd move error 0.04 0.02) Finished aligning to item in 1 rounds Trimming A by 20 115 20 135, reference by 20 115 20 135 -0.4 -4.1 unbinned pixels; 14.7 7.7 nm along two shift axes; 16.2 -0.8 nm on specimen axes Total_Shift = 8.313835 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.91 microns changed by -2.51 to target drift = 0.14 nm/sec Measured defocus = -1.63 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2384.tif 27 frames are being saved to U:\krios\20190717\PilA_2385.tif 27 frames are being saved to U:\krios\20190717\PilA_2386.tif 27 frames are being saved to U:\krios\20190717\PilA_2387.tif 27 frames are being saved to U:\krios\20190717\PilA_2388.tif 27 frames are being saved to U:\krios\20190717\PilA_2389.tif Running script 17 at item 468 (-411.87, -230.41) Remaining time to fill dewars is 14880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 90 20 165, reference by 23 145 20 160 Disparity in stage position after aligning to target position: -0.70 0.33 (2nd move error -0.46 0.56) Finished aligning to item in 1 rounds Trimming A by 30 72 20 170, reference by 21 90 20 165 9.9 86.9 unbinned pixels; 306.6 166.4 nm along two shift axes; -340.1 20.2 nm on specimen axes Total_Shift = 174.915067 Trimming A by 55 85 20 160, reference by 21 90 20 165 8.6 55.5 unbinned pixels; 192.9 114.5 nm along two shift axes; -217.8 21.7 nm on specimen axes Total_Shift = 112.361505 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 2.24 microns changed by -3.94 to target drift = 0.23 nm/sec Measured defocus = -1.74 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2390.tif 27 frames are being saved to U:\krios\20190717\PilA_2391.tif 27 frames are being saved to U:\krios\20190717\PilA_2392.tif 27 frames are being saved to U:\krios\20190717\PilA_2393.tif 27 frames are being saved to U:\krios\20190717\PilA_2394.tif 27 frames are being saved to U:\krios\20190717\PilA_2395.tif Running script 17 at item 469 (-253.15, -89.22) Remaining time to fill dewars is 14700 Dewars are NOT busy filling Trimming A by 239 22 195 20, reference by 220 22 20 215 Trimming A by 239 22 195 20, reference by 231 22 175 20 Trimming A by 239 22 195 20, reference by 23 180 20 185 Trimming A by 239 22 195 20, reference by 24 185 175 20 Disparity in stage position after aligning to center of map frame: -0.47 -0.22 Trimming A by 105 36 155 20, reference by 231 22 175 20 Disparity in stage position after aligning to target position: -0.37 -0.11 (2nd move error 0.08 0.09) Finished aligning to item in 2 rounds Trimming A by 105 31 160 20, reference by 105 36 155 20 -0.3 -0.0 unbinned pixels; 0.3 1.1 nm along two shift axes; 0.2 -1.2 nm on specimen axes Total_Shift = 0.609051 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = -0.79 microns changed by -1.01 to target drift = 0.20 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2396.tif 27 frames are being saved to U:\krios\20190717\PilA_2397.tif 27 frames are being saved to U:\krios\20190717\PilA_2398.tif 27 frames are being saved to U:\krios\20190717\PilA_2399.tif 27 frames are being saved to U:\krios\20190717\PilA_2400.tif 27 frames are being saved to U:\krios\20190717\PilA_2401.tif Running script 17 at item 470 (-255.66, -87.52) Remaining time to fill dewars is 14520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 120 23 125 20, reference by 231 22 175 20 Disparity in stage position after aligning to target position: -0.37 -0.32 (2nd move error 0.09 -0.12) Finished aligning to item in 1 rounds Trimming A by 120 23 125 20, reference by 120 23 125 20 -15.4 -3.4 unbinned pixels; 7.6 60.7 nm along two shift axes; 16.5 -59.3 nm on specimen axes Total_Shift = 31.624054 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.61 microns changed by -2.51 to target drift = 0.13 nm/sec Measured defocus = -1.88 microns changed by -0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2402.tif 27 frames are being saved to U:\krios\20190717\PilA_2403.tif 27 frames are being saved to U:\krios\20190717\PilA_2404.tif 27 frames are being saved to U:\krios\20190717\PilA_2405.tif 27 frames are being saved to U:\krios\20190717\PilA_2406.tif 27 frames are being saved to U:\krios\20190717\PilA_2407.tif Running script 17 at item 471 (-258.20, -85.76) Remaining time to fill dewars is 14280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 23 100 20, reference by 231 22 175 20 Disparity in stage position after aligning to target position: -0.44 -0.34 (2nd move error 0.02 -0.14) Finished aligning to item in 1 rounds Trimming A by 140 23 100 20, reference by 140 23 100 20 -17.1 -0.7 unbinned pixels; 19.8 62.6 nm along two shift axes; 6.3 -66.4 nm on specimen axes Total_Shift = 34.250933 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.54 microns changed by -2.54 to target drift = 0.10 nm/sec Measured defocus = -2.02 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2408.tif 27 frames are being saved to U:\krios\20190717\PilA_2409.tif 27 frames are being saved to U:\krios\20190717\PilA_2410.tif 27 frames are being saved to U:\krios\20190717\PilA_2411.tif 27 frames are being saved to U:\krios\20190717\PilA_2412.tif 27 frames are being saved to U:\krios\20190717\PilA_2413.tif Running script 17 at item 472 (-260.70, -84.08) Remaining time to fill dewars is 14100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 160 20 80 50, reference by 231 22 175 20 Disparity in stage position after aligning to target position: -0.47 -0.38 (2nd move error -0.01 -0.18) Finished aligning to item in 1 rounds Trimming A by 160 20 75 45, reference by 160 20 80 50 -24.2 4.7 unbinned pixels; 48.9 80.1 nm along two shift axes; -13.2 -95.3 nm on specimen axes Total_Shift = 49.363482 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.50 microns changed by -2.60 to target drift = 0.05 nm/sec Measured defocus = -2.14 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2414.tif 27 frames are being saved to U:\krios\20190717\PilA_2415.tif 27 frames are being saved to U:\krios\20190717\PilA_2416.tif 27 frames are being saved to U:\krios\20190717\PilA_2417.tif 27 frames are being saved to U:\krios\20190717\PilA_2418.tif 27 frames are being saved to U:\krios\20190717\PilA_2419.tif Running script 17 at item 473 (-262.31, -87.14) Remaining time to fill dewars is 13920 Dewars are NOT busy filling Trimming A by 225 23 20 205, reference by 220 22 20 215 Trimming A by 225 23 20 205, reference by 231 22 175 20 Trimming A by 225 23 20 205, reference by 23 180 20 185 Trimming A by 225 23 20 205, reference by 24 185 175 20 Disparity in stage position after aligning to center of map frame: -0.39 -0.13 Trimming A by 95 24 20 75, reference by 220 22 20 215 Disparity in stage position after aligning to target position: -0.34 0.01 (2nd move error 0.04 0.13) Finished aligning to item in 2 rounds Trimming A by 95 20 20 75, reference by 95 24 20 75 0.5 1.0 unbinned pixels; 3.2 3.3 nm along two shift axes; -4.2 1.6 nm on specimen axes Total_Shift = 2.302983 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.28 microns changed by -2.48 to target drift = 0.13 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2420.tif 27 frames are being saved to U:\krios\20190717\PilA_2421.tif 27 frames are being saved to U:\krios\20190717\PilA_2422.tif 27 frames are being saved to U:\krios\20190717\PilA_2423.tif 27 frames are being saved to U:\krios\20190717\PilA_2424.tif 27 frames are being saved to U:\krios\20190717\PilA_2425.tif Running script 17 at item 474 (-259.77, -88.37) Remaining time to fill dewars is 13740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 20 45 20, reference by 231 22 175 20 Disparity in stage position after aligning to target position: -0.28 -0.35 (2nd move error 0.18 -0.15) Finished aligning to item in 1 rounds Trimming A by 55 20 45 20, reference by 55 20 45 20 -14.8 0.9 unbinned pixels; 22.5 52.0 nm along two shift axes; -0.2 -57.7 nm on specimen axes Total_Shift = 29.631232 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.20 microns changed by -2.49 to target drift = 0.14 nm/sec Measured defocus = -2.33 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2426.tif 27 frames are being saved to U:\krios\20190717\PilA_2427.tif 27 frames are being saved to U:\krios\20190717\PilA_2428.tif 27 frames are being saved to U:\krios\20190717\PilA_2429.tif 27 frames are being saved to U:\krios\20190717\PilA_2430.tif 27 frames are being saved to U:\krios\20190717\PilA_2431.tif Running script 17 at item 475 (-257.33, -90.10) Remaining time to fill dewars is 13560 Dewars are NOT busy filling Trimming A by 241 24 185 20, reference by 220 22 20 215 Trimming A by 241 24 185 20, reference by 231 22 175 20 Trimming A by 241 24 185 20, reference by 23 180 20 185 Trimming A by 241 24 185 20, reference by 24 185 175 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.19 Trimming A by 50 20 55 20, reference by 231 22 175 20 Disparity in stage position after aligning to target position: -0.34 -0.36 (2nd move error 0.04 -0.18) Finished aligning to item in 2 rounds Trimming A by 50 20 55 20, reference by 50 20 55 20 -19.1 -8.0 unbinned pixels; 4.4 80.6 nm along two shift axes; 35.0 -72.5 nm on specimen axes Total_Shift = 41.330903 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.00 microns changed by -2.40 to target drift = 0.17 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2432.tif 27 frames are being saved to U:\krios\20190717\PilA_2433.tif 27 frames are being saved to U:\krios\20190717\PilA_2434.tif 27 frames are being saved to U:\krios\20190717\PilA_2435.tif 27 frames are being saved to U:\krios\20190717\PilA_2436.tif 27 frames are being saved to U:\krios\20190717\PilA_2437.tif Running script 17 at item 476 (-254.69, -91.65) Remaining time to fill dewars is 13320 Dewars are NOT busy filling Trimming A by 24 185 180 20, reference by 220 22 20 215 Trimming A by 24 185 180 20, reference by 231 22 175 20 Trimming A by 24 185 180 20, reference by 23 180 20 185 Trimming A by 24 185 180 20, reference by 24 185 175 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.25 Trimming A by 24 49 135 20, reference by 24 185 175 20 Disparity in stage position after aligning to target position: -0.37 -0.33 (2nd move error -0.03 -0.11) Finished aligning to item in 2 rounds Trimming A by 24 65 125 20, reference by 24 49 135 20 -16.0 7.8 unbinned pixels; 49.7 45.9 nm along two shift axes; -27.1 -64.0 nm on specimen axes Total_Shift = 35.689572 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.16 microns changed by -2.34 to target drift = 0.02 nm/sec Measured defocus = -2.55 microns changed by 0.05 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2438.tif 27 frames are being saved to U:\krios\20190717\PilA_2439.tif 27 frames are being saved to U:\krios\20190717\PilA_2440.tif 27 frames are being saved to U:\krios\20190717\PilA_2441.tif 27 frames are being saved to U:\krios\20190717\PilA_2442.tif 27 frames are being saved to U:\krios\20190717\PilA_2443.tif Running script 17 at item 477 (-256.26, -94.23) Remaining time to fill dewars is 13140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 95 105 20, reference by 24 185 175 20 Disparity in stage position after aligning to target position: -0.33 -0.41 (2nd move error 0.00 -0.19) Finished aligning to item in 1 rounds Trimming A by 23 105 100 20, reference by 20 95 105 20 -21.3 2.1 unbinned pixels; 35.4 73.6 nm along two shift axes; -3.5 -83.3 nm on specimen axes Total_Shift = 42.816133 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.25 microns changed by -2.35 to target drift = 0.13 nm/sec Measured defocus = -2.64 microns changed by 0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2444.tif 27 frames are being saved to U:\krios\20190717\PilA_2445.tif 27 frames are being saved to U:\krios\20190717\PilA_2446.tif 27 frames are being saved to U:\krios\20190717\PilA_2447.tif 27 frames are being saved to U:\krios\20190717\PilA_2448.tif 27 frames are being saved to U:\krios\20190717\PilA_2449.tif Running script 17 at item 478 (-258.78, -92.59) Remaining time to fill dewars is 12960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 35 35 20, reference by 24 185 175 20 Disparity in stage position after aligning to target position: -0.41 -0.46 (2nd move error -0.07 -0.23) Finished aligning to item in 1 rounds Trimming A by 20 40 35 20, reference by 21 35 35 20 -26.7 14.1 unbinned pixels; 86.8 75.1 nm along two shift axes; -49.2 -107.0 nm on specimen axes Total_Shift = 60.469691 Trimming A by 20 40 35 20, reference by 21 35 35 20 -0.3 7.3 unbinned pixels; 27.3 9.9 nm along two shift axes; -28.4 -2.7 nm on specimen axes Total_Shift = 14.640083 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 0.12 microns changed by -1.22 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2450.tif 27 frames are being saved to U:\krios\20190717\PilA_2451.tif 27 frames are being saved to U:\krios\20190717\PilA_2452.tif 27 frames are being saved to U:\krios\20190717\PilA_2453.tif 27 frames are being saved to U:\krios\20190717\PilA_2454.tif 27 frames are being saved to U:\krios\20190717\PilA_2455.tif Running script 17 at item 479 (-261.26, -91.33) Remaining time to fill dewars is 12780 Dewars are NOT busy filling Trimming A by 22 157 20 195, reference by 220 22 20 215 Trimming A by 22 157 20 195, reference by 231 22 175 20 Trimming A by 22 157 20 195, reference by 23 180 20 185 Trimming A by 22 157 20 195, reference by 24 185 175 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.20 Trimming A by 20 20 20 20, reference by 23 180 20 185 Disparity in stage position after aligning to target position: -0.41 0.05 (2nd move error -0.04 0.22) Finished aligning to item in 2 rounds -6.7 27.8 unbinned pixels; 110.8 17.6 nm along two shift axes; -106.6 -32.0 nm on specimen axes Total_Shift = 57.11543 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.35 microns changed by -2.55 to target drift = 0.09 nm/sec Measured defocus = -1.19 microns changed by -0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2456.tif 27 frames are being saved to U:\krios\20190717\PilA_2457.tif 27 frames are being saved to U:\krios\20190717\PilA_2458.tif 27 frames are being saved to U:\krios\20190717\PilA_2459.tif 27 frames are being saved to U:\krios\20190717\PilA_2460.tif 27 frames are being saved to U:\krios\20190717\PilA_2461.tif Running script 17 at item 480 (-263.94, -89.68) Remaining time to fill dewars is 12540 Dewars are NOT busy filling Trimming A by 211 22 20 220, reference by 220 22 20 215 Trimming A by 211 22 20 220, reference by 231 22 175 20 Trimming A by 211 22 20 220, reference by 23 180 20 185 Trimming A by 211 22 20 220, reference by 24 185 175 20 Disparity in stage position after aligning to center of map frame: -0.33 -0.17 Trimming A by 61 20 20 95, reference by 220 22 20 215 Disparity in stage position after aligning to target position: -0.20 -0.04 (2nd move error 0.12 0.10) Finished aligning to item in 2 rounds Trimming A by 52 22 20 100, reference by 61 20 20 95 1.0 -2.9 unbinned pixels; 11.7 0.9 nm along two shift axes; 10.9 4.3 nm on specimen axes Total_Shift = 6.01196 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.22 microns changed by -2.52 to target drift = 0.12 nm/sec Measured defocus = -1.26 microns changed by -0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2462.tif 27 frames are being saved to U:\krios\20190717\PilA_2463.tif 27 frames are being saved to U:\krios\20190717\PilA_2464.tif 27 frames are being saved to U:\krios\20190717\PilA_2465.tif 27 frames are being saved to U:\krios\20190717\PilA_2466.tif 27 frames are being saved to U:\krios\20190717\PilA_2467.tif Running script 17 at item 481 (-266.43, -88.04) Remaining time to fill dewars is 12360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 112 22 20 175, reference by 220 22 20 215 Disparity in stage position after aligning to target position: -0.24 -0.19 (2nd move error 0.08 -0.03) Finished aligning to item in 1 rounds Trimming A by 109 20 20 175, reference by 112 22 20 175 -2.9 -1.9 unbinned pixels; 3.3 13.2 nm along two shift axes; 8.1 -10.8 nm on specimen axes Total_Shift = 6.921517 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.16 microns changed by -2.56 to target drift = 0.09 nm/sec Measured defocus = -1.41 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2468.tif 27 frames are being saved to U:\krios\20190717\PilA_2469.tif 27 frames are being saved to U:\krios\20190717\PilA_2470.tif 27 frames are being saved to U:\krios\20190717\PilA_2471.tif 27 frames are being saved to U:\krios\20190717\PilA_2472.tif 27 frames are being saved to U:\krios\20190717\PilA_2473.tif Running script 17 at item 482 (-265.35, -92.28) Remaining time to fill dewars is 12240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 36 36 20 110, reference by 23 180 20 185 Disparity in stage position after aligning to target position: -0.40 -0.18 (2nd move error -0.03 0.00) Finished aligning to item in 1 rounds Trimming A by 36 36 20 110, reference by 36 36 20 110 1.0 6.4 unbinned pixels; 22.3 13.4 nm along two shift axes; -25.2 2.7 nm on specimen axes Total_Shift = 13.029283 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.11 microns changed by -2.61 to target drift = 0.11 nm/sec Measured defocus = -1.57 microns changed by 0.08 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2474.tif 27 frames are being saved to U:\krios\20190717\PilA_2475.tif 27 frames are being saved to U:\krios\20190717\PilA_2476.tif 27 frames are being saved to U:\krios\20190717\PilA_2477.tif 27 frames are being saved to U:\krios\20190717\PilA_2478.tif 27 frames are being saved to U:\krios\20190717\PilA_2479.tif Running script 17 at item 483 (-262.88, -93.99) Remaining time to fill dewars is 12000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 65 20 80, reference by 23 180 20 185 Disparity in stage position after aligning to target position: -0.27 -0.08 (2nd move error 0.09 0.10) Finished aligning to item in 1 rounds Trimming A by 21 60 20 75, reference by 21 65 20 80 0.8 4.4 unbinned pixels; 15.2 9.4 nm along two shift axes; -17.3 2.1 nm on specimen axes Total_Shift = 8.95377 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.79 microns changed by -2.39 to target drift = 0.12 nm/sec Measured defocus = -1.54 microns changed by -0.06 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2480.tif 27 frames are being saved to U:\krios\20190717\PilA_2481.tif 27 frames are being saved to U:\krios\20190717\PilA_2482.tif 27 frames are being saved to U:\krios\20190717\PilA_2483.tif 27 frames are being saved to U:\krios\20190717\PilA_2484.tif 27 frames are being saved to U:\krios\20190717\PilA_2485.tif Running script 17 at item 484 (-260.46, -95.17) Remaining time to fill dewars is 11760 Dewars are NOT busy filling Trimming A by 21 185 180 20, reference by 220 22 20 215 Trimming A by 21 185 180 20, reference by 231 22 175 20 Trimming A by 21 185 180 20, reference by 23 180 20 185 Trimming A by 21 185 180 20, reference by 24 185 175 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.20 Trimming A by 20 70 25 55, reference by 24 185 175 20 Disparity in stage position after aligning to target position: -0.29 -0.53 (2nd move error 0.02 -0.35) Finished aligning to item in 2 rounds Trimming A by 20 75 25 60, reference by 20 70 25 55 -30.9 0.6 unbinned pixels; 42.5 110.5 nm along two shift axes; 4.3 -120.4 nm on specimen axes Total_Shift = 61.863796 Trimming A by 20 80 25 60, reference by 20 70 25 55 -0.8 -2.1 unbinned pixels; 6.6 6.0 nm along two shift axes; 8.3 -2.7 nm on specimen axes Total_Shift = 4.459676 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.74 microns changed by -2.44 to target drift = 0.08 nm/sec Measured defocus = -1.68 microns changed by -0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2486.tif 27 frames are being saved to U:\krios\20190717\PilA_2487.tif 27 frames are being saved to U:\krios\20190717\PilA_2488.tif 27 frames are being saved to U:\krios\20190717\PilA_2489.tif 27 frames are being saved to U:\krios\20190717\PilA_2490.tif 27 frames are being saved to U:\krios\20190717\PilA_2491.tif Running script 17 at item 485 (-257.92, -96.88) Remaining time to fill dewars is 11580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 135 90 20, reference by 24 185 175 20 Disparity in stage position after aligning to target position: -0.29 -0.44 (2nd move error 0.01 -0.24) Finished aligning to item in 1 rounds Trimming A by 21 121 105 20, reference by 23 135 90 20 -26.7 -0.7 unbinned pixels; 32.4 97.0 nm along two shift axes; 8.2 -103.6 nm on specimen axes Total_Shift = 53.368175 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.54 microns changed by -2.34 to target drift = 0.12 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2492.tif 27 frames are being saved to U:\krios\20190717\PilA_2493.tif 27 frames are being saved to U:\krios\20190717\PilA_2494.tif 27 frames are being saved to U:\krios\20190717\PilA_2495.tif 27 frames are being saved to U:\krios\20190717\PilA_2496.tif 27 frames are being saved to U:\krios\20190717\PilA_2497.tif Running script 17 at item 486 (-261.95, -98.26) Remaining time to fill dewars is 11400 Dewars are NOT busy filling Trimming A by 24 170 20 185, reference by 220 22 20 215 Trimming A by 24 170 20 185, reference by 231 22 175 20 Trimming A by 24 170 20 185, reference by 23 180 20 185 Trimming A by 24 170 20 185, reference by 24 185 175 20 Disparity in stage position after aligning to center of map frame: -0.33 -0.23 Trimming A by 21 160 80 115, reference by 23 180 20 185 Disparity in stage position after aligning to target position: -0.30 0.01 (2nd move error 0.02 0.21) Finished aligning to item in 2 rounds Trimming A by 24 155 20 115, reference by 21 160 80 115 1.4 0.7 unbinned pixels; 0.8 6.0 nm along two shift axes; -3.0 5.1 nm on specimen axes Total_Shift = 3.063472 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.50 microns changed by -2.40 to target drift = 0.09 nm/sec Measured defocus = -1.93 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2498.tif 27 frames are being saved to U:\krios\20190717\PilA_2499.tif 27 frames are being saved to U:\krios\20190717\PilA_2500.tif 27 frames are being saved to U:\krios\20190717\PilA_2501.tif 27 frames are being saved to U:\krios\20190717\PilA_2502.tif 27 frames are being saved to U:\krios\20190717\PilA_2503.tif Running script 17 at item 487 (-264.50, -96.55) Remaining time to fill dewars is 11280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 135 20 135, reference by 23 180 20 185 Disparity in stage position after aligning to target position: -0.31 -0.17 (2nd move error 0.01 0.04) Finished aligning to item in 1 rounds Trimming A by 23 135 20 135, reference by 23 135 20 135 -2.7 -0.5 unbinned pixels; 1.8 10.4 nm along two shift axes; 2.4 -10.4 nm on specimen axes Total_Shift = 5.476546 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.40 microns changed by -2.40 to target drift = 0.10 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2504.tif 27 frames are being saved to U:\krios\20190717\PilA_2505.tif 27 frames are being saved to U:\krios\20190717\PilA_2506.tif 27 frames are being saved to U:\krios\20190717\PilA_2507.tif 27 frames are being saved to U:\krios\20190717\PilA_2508.tif 27 frames are being saved to U:\krios\20190717\PilA_2509.tif Running script 17 at item 488 (-266.97, -94.83) Remaining time to fill dewars is 10980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 102 20 180, reference by 23 180 20 185 Disparity in stage position after aligning to target position: -0.28 -0.27 (2nd move error 0.04 -0.04) Finished aligning to item in 1 rounds Trimming A by 22 110 20 170, reference by 23 102 20 180 -6.4 -0.1 unbinned pixels; 7.9 23.3 nm along two shift axes; 1.9 -24.9 nm on specimen axes Total_Shift = 12.845083 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.38 microns changed by -2.48 to target drift = 0.10 nm/sec Measured defocus = -2.14 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2510.tif 27 frames are being saved to U:\krios\20190717\PilA_2511.tif 27 frames are being saved to U:\krios\20190717\PilA_2512.tif 27 frames are being saved to U:\krios\20190717\PilA_2513.tif 27 frames are being saved to U:\krios\20190717\PilA_2514.tif 27 frames are being saved to U:\krios\20190717\PilA_2515.tif Running script 17 at item 489 (-267.96, -90.39) Remaining time to fill dewars is 10800 Dewars are NOT busy filling Trimming A by 220 22 20 215, reference by 220 22 20 215 Trimming A by 220 22 20 215, reference by 231 22 175 20 Trimming A by 220 22 20 215, reference by 23 180 20 185 Trimming A by 220 22 20 215, reference by 24 185 175 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.18 Trimming A by 83 23 20 195, reference by 220 22 20 215 Disparity in stage position after aligning to target position: -0.16 -0.21 (2nd move error 0.06 -0.06) Finished aligning to item in 2 rounds Trimming A by 84 22 20 195, reference by 83 23 20 195 -8.8 -5.1 unbinned pixels; 7.1 39.5 nm along two shift axes; 21.6 -33.3 nm on specimen axes Total_Shift = 20.387541 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.33 microns changed by -2.53 to target drift = 0.13 nm/sec Measured defocus = -2.25 microns changed by 0.05 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2516.tif 27 frames are being saved to U:\krios\20190717\PilA_2517.tif 27 frames are being saved to U:\krios\20190717\PilA_2518.tif 27 frames are being saved to U:\krios\20190717\PilA_2519.tif 27 frames are being saved to U:\krios\20190717\PilA_2520.tif 27 frames are being saved to U:\krios\20190717\PilA_2521.tif Running script 17 at item 490 (-121.40, -235.41) Remaining time to fill dewars is 10620 Dewars are NOT busy filling Trimming A by 255 21 155 20, reference by 215 20 20 220 Trimming A by 255 21 155 20, reference by 240 21 150 20 Trimming A by 255 21 155 20, reference by 21 108 20 185 Trimming A by 255 21 155 20, reference by 23 135 145 20 Disparity in stage position after aligning to center of map frame: -0.40 -0.27 Trimming A by 135 21 135 20, reference by 240 21 150 20 Disparity in stage position after aligning to target position: -0.29 -0.18 (2nd move error 0.10 0.07) Finished aligning to item in 2 rounds Trimming A by 135 22 140 20, reference by 135 21 135 20 -0.8 2.5 unbinned pixels; 10.0 0.9 nm along two shift axes; -9.4 -3.5 nm on specimen axes Total_Shift = 5.13252 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.06 microns changed by -2.36 to target drift = 0.20 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2522.tif 27 frames are being saved to U:\krios\20190717\PilA_2523.tif 27 frames are being saved to U:\krios\20190717\PilA_2524.tif 27 frames are being saved to U:\krios\20190717\PilA_2525.tif 27 frames are being saved to U:\krios\20190717\PilA_2526.tif 27 frames are being saved to U:\krios\20190717\PilA_2527.tif Running script 17 at item 491 (-123.97, -233.72) Remaining time to fill dewars is 10440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 22 110 20, reference by 240 21 150 20 Disparity in stage position after aligning to target position: -0.33 -0.38 (2nd move error 0.06 -0.13) Finished aligning to item in 1 rounds Trimming A by 150 22 110 20, reference by 150 22 110 20 -16.3 -1.0 unbinned pixels; 17.4 60.1 nm along two shift axes; 7.5 -63.0 nm on specimen axes Total_Shift = 32.583059 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.05 microns changed by -2.45 to target drift = 0.12 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2528.tif 27 frames are being saved to U:\krios\20190717\PilA_2529.tif 27 frames are being saved to U:\krios\20190717\PilA_2530.tif 27 frames are being saved to U:\krios\20190717\PilA_2531.tif 27 frames are being saved to U:\krios\20190717\PilA_2532.tif 27 frames are being saved to U:\krios\20190717\PilA_2533.tif Running script 17 at item 492 (-126.41, -232.04) Remaining time to fill dewars is 10320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 165 21 115 20, reference by 240 21 150 20 Disparity in stage position after aligning to target position: -0.32 -0.44 (2nd move error 0.07 -0.19) Finished aligning to item in 1 rounds Trimming A by 160 22 110 20, reference by 165 21 115 20 -24.0 -5.1 unbinned pixels; 12.6 94.0 nm along two shift axes; 24.8 -92.2 nm on specimen axes Total_Shift = 49.038497 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.01 microns changed by -2.49 to target drift = 0.19 nm/sec Measured defocus = -2.52 microns changed by 0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2534.tif 27 frames are being saved to U:\krios\20190717\PilA_2535.tif 27 frames are being saved to U:\krios\20190717\PilA_2536.tif 27 frames are being saved to U:\krios\20190717\PilA_2537.tif 27 frames are being saved to U:\krios\20190717\PilA_2538.tif 27 frames are being saved to U:\krios\20190717\PilA_2539.tif Running script 17 at item 493 (-128.96, -230.32) Remaining time to fill dewars is 10140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 205 23 125 35, reference by 240 21 150 20 Disparity in stage position after aligning to target position: -0.41 -0.53 (2nd move error -0.01 -0.28) Finished aligning to item in 1 rounds Trimming A by 195 20 125 40, reference by 205 23 125 35 -29.5 5.2 unbinned pixels; 57.7 98.4 nm along two shift axes; -14.1 -115.9 nm on specimen axes Total_Shift = 59.93349 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -1.26 microns changed by -1.34 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2540.tif 27 frames are being saved to U:\krios\20190717\PilA_2541.tif 27 frames are being saved to U:\krios\20190717\PilA_2542.tif 27 frames are being saved to U:\krios\20190717\PilA_2543.tif 27 frames are being saved to U:\krios\20190717\PilA_2544.tif 27 frames are being saved to U:\krios\20190717\PilA_2545.tif Running script 17 at item 494 (-130.63, -233.42) Remaining time to fill dewars is 9960 Dewars are NOT busy filling Trimming A by 220 21 20 215, reference by 215 20 20 220 Trimming A by 220 21 20 215, reference by 240 21 150 20 Trimming A by 220 21 20 215, reference by 21 108 20 185 Trimming A by 220 21 20 215, reference by 23 135 145 20 Disparity in stage position after aligning to center of map frame: -0.30 -0.17 Trimming A by 100 20 70 100, reference by 215 20 20 220 Disparity in stage position after aligning to target position: -0.01 -0.04 (2nd move error 0.28 0.10) Finished aligning to item in 2 rounds Trimming A by 115 21 70 85, reference by 100 20 70 100 1.7 -4.4 unbinned pixels; 18.2 0.5 nm along two shift axes; 16.6 7.5 nm on specimen axes Total_Shift = 9.330675 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.46 microns changed by -2.56 to target drift = 0.13 nm/sec Measured defocus = -1.08 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2546.tif 27 frames are being saved to U:\krios\20190717\PilA_2547.tif 27 frames are being saved to U:\krios\20190717\PilA_2548.tif 27 frames are being saved to U:\krios\20190717\PilA_2549.tif 27 frames are being saved to U:\krios\20190717\PilA_2550.tif 27 frames are being saved to U:\krios\20190717\PilA_2551.tif Running script 17 at item 495 (-125.51, -236.27) Remaining time to fill dewars is 9660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 20 55 20, reference by 240 21 150 20 Disparity in stage position after aligning to target position: -0.18 -0.33 (2nd move error 0.21 -0.08) Finished aligning to item in 1 rounds Trimming A by 65 20 60 20, reference by 65 20 55 20 -5.6 0.8 unbinned pixels; 10.1 19.0 nm along two shift axes; -1.8 -21.9 nm on specimen axes Total_Shift = 11.293096 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.30 microns changed by -2.50 to target drift = 0.09 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2552.tif 27 frames are being saved to U:\krios\20190717\PilA_2553.tif 27 frames are being saved to U:\krios\20190717\PilA_2554.tif 27 frames are being saved to U:\krios\20190717\PilA_2555.tif 27 frames are being saved to U:\krios\20190717\PilA_2556.tif 27 frames are being saved to U:\krios\20190717\PilA_2557.tif Running script 17 at item 496 (-122.86, -237.98) Remaining time to fill dewars is 9480 Dewars are NOT busy filling Trimming A by 22 131 150 20, reference by 215 20 20 220 Trimming A by 22 131 150 20, reference by 240 21 150 20 Trimming A by 22 131 150 20, reference by 21 108 20 185 Trimming A by 22 131 150 20, reference by 23 135 145 20 Disparity in stage position after aligning to center of map frame: -0.27 -0.26 Trimming A by 60 36 75 30, reference by 23 135 145 20 Disparity in stage position after aligning to target position: -0.39 -0.34 (2nd move error -0.12 -0.11) Finished aligning to item in 2 rounds Trimming A by 60 36 75 30, reference by 60 36 75 30 -19.0 33.6 unbinned pixels; 148.3 17.8 nm along two shift axes; -126.7 -81.0 nm on specimen axes Total_Shift = 77.173057 Trimming A by 60 45 70 20, reference by 60 36 75 30 -6.0 23.5 unbinned pixels; 94.2 13.7 nm along two shift axes; -90.0 -28.4 nm on specimen axes Total_Shift = 48.462006 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.14 microns changed by -2.44 to target drift = 0.05 nm/sec Measured defocus = -1.29 microns changed by -0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2558.tif 27 frames are being saved to U:\krios\20190717\PilA_2559.tif 27 frames are being saved to U:\krios\20190717\PilA_2560.tif 27 frames are being saved to U:\krios\20190717\PilA_2561.tif 27 frames are being saved to U:\krios\20190717\PilA_2562.tif 27 frames are being saved to U:\krios\20190717\PilA_2563.tif Running script 17 at item 497 (-120.32, -239.68) Remaining time to fill dewars is 9300 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 21 86 155 20, reference by 23 135 145 20 Disparity in stage position after aligning to target position: -0.40 -0.25 (2nd move error -0.13 -0.01) Finished aligning to item in 1 rounds Trimming A by 23 69 165 20, reference by 21 86 155 20 -0.8 26.7 unbinned pixels; 99.1 37.4 nm along two shift axes; -103.7 -8.6 nm on specimen axes Total_Shift = 53.395329 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.00 microns changed by -2.40 to target drift = 0.08 nm/sec Measured defocus = -1.41 microns changed by 0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2564.tif 27 frames are being saved to U:\krios\20190717\PilA_2565.tif 27 frames are being saved to U:\krios\20190717\PilA_2566.tif 27 frames are being saved to U:\krios\20190717\PilA_2567.tif 27 frames are being saved to U:\krios\20190717\PilA_2568.tif 27 frames are being saved to U:\krios\20190717\PilA_2569.tif Running script 17 at item 498 (-121.95, -242.32) Remaining time to fill dewars is 9120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 110 125 20, reference by 23 135 145 20 Disparity in stage position after aligning to target position: -0.27 -0.36 (2nd move error -0.01 -0.13) Finished aligning to item in 1 rounds Trimming A by 22 115 125 20, reference by 24 110 125 20 -14.3 3.3 unbinned pixels; 30.8 46.5 nm along two shift axes; -9.8 -56.3 nm on specimen axes Total_Shift = 29.329235 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.89 microns changed by -2.39 to target drift = 0.05 nm/sec Measured defocus = -1.49 microns changed by -0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2570.tif 27 frames are being saved to U:\krios\20190717\PilA_2571.tif 27 frames are being saved to U:\krios\20190717\PilA_2572.tif 27 frames are being saved to U:\krios\20190717\PilA_2573.tif 27 frames are being saved to U:\krios\20190717\PilA_2574.tif 27 frames are being saved to U:\krios\20190717\PilA_2575.tif Running script 17 at item 499 (-124.48, -240.56) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 32 65 20, reference by 23 135 145 20 Disparity in stage position after aligning to target position: -0.33 -0.45 (2nd move error -0.07 -0.22) Finished aligning to item in 1 rounds Trimming A by 24 41 60 20, reference by 22 32 65 20 -30.1 19.0 unbinned pixels; 109.3 79.9 nm along two shift axes; -67.7 -121.3 nm on specimen axes Total_Shift = 71.288986 Trimming A by 24 41 60 20, reference by 22 32 65 20 -6.0 11.4 unbinned pixels; 49.8 4.4 nm along two shift axes; -43.1 -25.7 nm on specimen axes Total_Shift = 25.766648 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.86 microns changed by -2.46 to target drift = 0.06 nm/sec Measured defocus = -1.64 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2576.tif 27 frames are being saved to U:\krios\20190717\PilA_2577.tif 27 frames are being saved to U:\krios\20190717\PilA_2578.tif 27 frames are being saved to U:\krios\20190717\PilA_2579.tif 27 frames are being saved to U:\krios\20190717\PilA_2580.tif 27 frames are being saved to U:\krios\20190717\PilA_2581.tif Running script 17 at item 500 (-127.05, -238.76) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 135 145 20 Disparity in stage position after aligning to target position: -0.40 -0.54 (2nd move error -0.13 -0.30) Finished aligning to item in 1 rounds -27.8 24.6 unbinned pixels; 126.7 63.3 nm along two shift axes; -89.7 -113.5 nm on specimen axes Total_Shift = 74.258154 -0.5 15.7 unbinned pixels; 58.5 22.0 nm along two shift axes; -61.2 -5.1 nm on specimen axes Total_Shift = 31.512415 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.84 microns changed by -2.54 to target drift = 0.02 nm/sec Measured defocus = -1.77 microns changed by 0.07 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2582.tif 27 frames are being saved to U:\krios\20190717\PilA_2583.tif 27 frames are being saved to U:\krios\20190717\PilA_2584.tif 27 frames are being saved to U:\krios\20190717\PilA_2585.tif 27 frames are being saved to U:\krios\20190717\PilA_2586.tif 27 frames are being saved to U:\krios\20190717\PilA_2587.tif Running script 17 at item 501 (-129.80, -237.38) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 220 21 20 215, reference by 215 20 20 220 Trimming A by 220 21 20 215, reference by 240 21 150 20 Trimming A by 220 21 20 215, reference by 21 108 20 185 Trimming A by 220 21 20 215, reference by 23 135 145 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.16 Trimming A by 30 20 20 30, reference by 215 20 20 220 Disparity in stage position after aligning to target position: 0.01 0.01 (2nd move error 0.29 0.16) Finished aligning to item in 2 rounds Trimming A by 30 20 20 25, reference by 30 20 20 30 2.6 -15.2 unbinned pixels; 59.2 13.5 nm along two shift axes; 58.5 13.3 nm on specimen axes Total_Shift = 30.786109 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.70 microns changed by -2.50 to target drift = 0.16 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2588.tif 27 frames are being saved to U:\krios\20190717\PilA_2589.tif 27 frames are being saved to U:\krios\20190717\PilA_2590.tif 27 frames are being saved to U:\krios\20190717\PilA_2591.tif 27 frames are being saved to U:\krios\20190717\PilA_2592.tif 27 frames are being saved to U:\krios\20190717\PilA_2593.tif Running script 17 at item 502 (-132.31, -236.02) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 85 20 20 100, reference by 215 20 20 220 Disparity in stage position after aligning to target position: -0.05 -0.15 (2nd move error 0.22 -0.00) Finished aligning to item in 1 rounds Trimming A by 80 20 20 95, reference by 85 20 20 100 1.4 2.9 unbinned pixels; 8.8 9.2 nm along two shift axes; -11.5 4.7 nm on specimen axes Total_Shift = 6.37552 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.63 microns changed by -2.53 to target drift = 0.11 nm/sec Measured defocus = -1.86 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2594.tif 27 frames are being saved to U:\krios\20190717\PilA_2595.tif 27 frames are being saved to U:\krios\20190717\PilA_2596.tif 27 frames are being saved to U:\krios\20190717\PilA_2597.tif 27 frames are being saved to U:\krios\20190717\PilA_2598.tif 27 frames are being saved to U:\krios\20190717\PilA_2599.tif Running script 17 at item 503 (-133.70, -238.56) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 21 125 20 165, reference by 215 20 20 220 Trimming A by 21 125 20 165, reference by 240 21 150 20 Trimming A by 21 125 20 165, reference by 21 108 20 185 Trimming A by 21 125 20 165, reference by 23 135 145 20 Disparity in stage position after aligning to center of map frame: -0.26 -0.24 Trimming A by 26 21 20 135, reference by 21 108 20 185 Disparity in stage position after aligning to target position: -0.30 -0.11 (2nd move error -0.05 0.11) Finished aligning to item in 2 rounds Trimming A by 26 21 20 135, reference by 26 21 20 135 1.8 10.9 unbinned pixels; 37.8 22.9 nm along two shift axes; -42.9 4.7 nm on specimen axes Total_Shift = 22.130442 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.52 microns changed by -2.52 to target drift = 0.15 nm/sec Measured defocus = -1.94 microns changed by -0.06 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2600.tif 27 frames are being saved to U:\krios\20190717\PilA_2601.tif 27 frames are being saved to U:\krios\20190717\PilA_2602.tif 27 frames are being saved to U:\krios\20190717\PilA_2603.tif 27 frames are being saved to U:\krios\20190717\PilA_2604.tif 27 frames are being saved to U:\krios\20190717\PilA_2605.tif Running script 17 at item 504 (-131.15, -240.30) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 40, reference by 21 108 20 185 Disparity in stage position after aligning to target position: -0.22 -0.08 (2nd move error 0.03 0.14) Finished aligning to item in 1 rounds Trimming A by 25 20 20 40, reference by 20 20 20 40 -2.9 -0.7 unbinned pixels; 1.2 11.6 nm along two shift axes; 3.4 -11.3 nm on specimen axes Total_Shift = 6.045979 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.43 microns changed by -2.53 to target drift = 0.09 nm/sec Measured defocus = -2.14 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2606.tif 27 frames are being saved to U:\krios\20190717\PilA_2607.tif 27 frames are being saved to U:\krios\20190717\PilA_2608.tif 27 frames are being saved to U:\krios\20190717\PilA_2609.tif 27 frames are being saved to U:\krios\20190717\PilA_2610.tif 27 frames are being saved to U:\krios\20190717\PilA_2611.tif Running script 17 at item 505 (-128.62, -241.42) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Trimming A by 22 131 150 20, reference by 215 20 20 220 Trimming A by 22 131 150 20, reference by 240 21 150 20 Trimming A by 22 131 150 20, reference by 21 108 20 185 Trimming A by 22 131 150 20, reference by 23 135 145 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.22 Trimming A by 20 20 20 20, reference by 23 135 145 20 Disparity in stage position after aligning to target position: -0.36 -0.58 (2nd move error -0.12 -0.39) Finished aligning to item in 2 rounds -39.8 23.9 unbinned pixels; 139.7 107.3 nm along two shift axes; -84.5 -159.8 nm on specimen axes Total_Shift = 92.778569 -3.2 14.1 unbinned pixels; 56.1 9.6 nm along two shift axes; -54.2 -15.6 nm on specimen axes Total_Shift = 28.967903 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.34 microns changed by -2.54 to target drift = 0.04 nm/sec Measured defocus = -2.27 microns changed by 0.07 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2612.tif 27 frames are being saved to U:\krios\20190717\PilA_2613.tif 27 frames are being saved to U:\krios\20190717\PilA_2614.tif 27 frames are being saved to U:\krios\20190717\PilA_2615.tif 27 frames are being saved to U:\krios\20190717\PilA_2616.tif 27 frames are being saved to U:\krios\20190717\PilA_2617.tif Running script 17 at item 506 (-123.62, -244.87) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 112 125 20, reference by 23 135 145 20 Disparity in stage position after aligning to target position: -0.27 -0.33 (2nd move error -0.03 -0.13) Finished aligning to item in 1 rounds Trimming A by 21 112 125 20, reference by 21 112 125 20 -16.8 9.1 unbinned pixels; 55.3 46.9 nm along two shift axes; -31.7 -67.4 nm on specimen axes Total_Shift = 38.230076 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.01 microns changed by -2.31 to target drift = 0.04 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2618.tif 27 frames are being saved to U:\krios\20190717\PilA_2619.tif 27 frames are being saved to U:\krios\20190717\PilA_2620.tif 27 frames are being saved to U:\krios\20190717\PilA_2621.tif 27 frames are being saved to U:\krios\20190717\PilA_2622.tif 27 frames are being saved to U:\krios\20190717\PilA_2623.tif Running script 17 at item 507 (-127.77, -245.74) Remaining time to fill dewars is 8880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 95 50 55, reference by 23 135 145 20 Disparity in stage position after aligning to target position: -0.24 -0.56 (2nd move error 0.01 -0.35) Finished aligning to item in 1 rounds Trimming A by 20 100 20 60, reference by 20 95 50 55 -33.4 -0.2 unbinned pixels; 43.0 120.4 nm along two shift axes; 7.6 -129.8 nm on specimen axes Total_Shift = 66.76447 Trimming A by 20 100 20 75, reference by 20 95 50 55 -2.8 -2.0 unbinned pixels; 3.6 13.2 nm along two shift axes; 8.3 -10.6 nm on specimen axes Total_Shift = 6.914167 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.01 microns changed by -2.41 to target drift = 0.13 nm/sec Measured defocus = -2.45 microns changed by 0.05 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2624.tif 27 frames are being saved to U:\krios\20190717\PilA_2625.tif 27 frames are being saved to U:\krios\20190717\PilA_2626.tif 27 frames are being saved to U:\krios\20190717\PilA_2627.tif 27 frames are being saved to U:\krios\20190717\PilA_2628.tif 27 frames are being saved to U:\krios\20190717\PilA_2629.tif Running script 17 at item 508 (-130.28, -244.55) Remaining time to fill dewars is 7680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 71 20 105, reference by 21 108 20 185 Disparity in stage position after aligning to target position: -0.09 -0.10 (2nd move error 0.16 0.12) Finished aligning to item in 1 rounds Trimming A by 24 71 20 100, reference by 20 71 20 105 0.4 -4.6 unbinned pixels; 17.6 5.5 nm along two shift axes; 18.0 2.6 nm on specimen axes Total_Shift = 9.3275 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.11 microns changed by -2.39 to target drift = 0.12 nm/sec Measured defocus = -2.47 microns changed by -0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2630.tif 27 frames are being saved to U:\krios\20190717\PilA_2631.tif 27 frames are being saved to U:\krios\20190717\PilA_2632.tif 27 frames are being saved to U:\krios\20190717\PilA_2633.tif 27 frames are being saved to U:\krios\20190717\PilA_2634.tif 27 frames are being saved to U:\krios\20190717\PilA_2635.tif Running script 17 at item 509 (-132.76, -242.88) Remaining time to fill dewars is 7500 Dewars are NOT busy filling Trimming A by 23 102 20 180, reference by 215 20 20 220 Trimming A by 23 102 20 180, reference by 240 21 150 20 Trimming A by 23 102 20 180, reference by 21 108 20 185 Trimming A by 23 102 20 180, reference by 23 135 145 20 Disparity in stage position after aligning to center of map frame: -0.26 -0.22 Trimming A by 21 80 30 105, reference by 21 108 20 185 Disparity in stage position after aligning to target position: -0.17 -0.09 (2nd move error 0.08 0.11) Finished aligning to item in 2 rounds Trimming A by 55 75 20 110, reference by 21 80 30 105 -2.2 0.9 unbinned pixels; 6.1 6.8 nm along two shift axes; -2.8 -8.9 nm on specimen axes Total_Shift = 4.805779 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -1.49 microns changed by -1.11 to target drift = 0.17 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2636.tif 27 frames are being saved to U:\krios\20190717\PilA_2637.tif 27 frames are being saved to U:\krios\20190717\PilA_2638.tif 27 frames are being saved to U:\krios\20190717\PilA_2639.tif 27 frames are being saved to U:\krios\20190717\PilA_2640.tif 27 frames are being saved to U:\krios\20190717\PilA_2641.tif Running script 17 at item 510 (-171.86, -716.48) Remaining time to fill dewars is 7320 Dewars are NOT busy filling Trimming A by 234 23 165 20, reference by 225 20 20 190 Trimming A by 234 23 165 20, reference by 225 20 155 20 Trimming A by 234 23 165 20, reference by 20 105 20 130 Trimming A by 234 23 165 20, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.30 -0.17 Trimming A by 122 22 180 20, reference by 225 20 155 20 Disparity in stage position after aligning to target position: -0.15 0.03 (2nd move error 0.14 0.19) Finished aligning to item in 2 rounds Trimming A by 145 24 170 20, reference by 122 22 180 20 5.8 -0.3 unbinned pixels; 8.6 20.6 nm along two shift axes; -0.2 22.7 nm on specimen axes Total_Shift = 11.646937 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.26 microns changed by -3.36 to target drift = 0.15 nm/sec Measured defocus = -1.13 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2642.tif 27 frames are being saved to U:\krios\20190717\PilA_2643.tif 27 frames are being saved to U:\krios\20190717\PilA_2644.tif 27 frames are being saved to U:\krios\20190717\PilA_2645.tif 27 frames are being saved to U:\krios\20190717\PilA_2646.tif 27 frames are being saved to U:\krios\20190717\PilA_2647.tif Running script 17 at item 511 (-174.29, -714.79) Remaining time to fill dewars is 7140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 155 24 140 20, reference by 225 20 155 20 Disparity in stage position after aligning to target position: -0.21 -0.21 (2nd move error 0.09 -0.05) Finished aligning to item in 1 rounds Trimming A by 155 20 145 20, reference by 155 24 140 20 -3.9 1.1 unbinned pixels; 9.0 12.5 nm along two shift axes; -3.3 -15.4 nm on specimen axes Total_Shift = 8.098426 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.18 microns changed by -3.38 to target drift = 0.04 nm/sec Measured defocus = -1.23 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2648.tif 27 frames are being saved to U:\krios\20190717\PilA_2649.tif 27 frames are being saved to U:\krios\20190717\PilA_2650.tif 27 frames are being saved to U:\krios\20190717\PilA_2651.tif 27 frames are being saved to U:\krios\20190717\PilA_2652.tif 27 frames are being saved to U:\krios\20190717\PilA_2653.tif Running script 17 at item 512 (-176.77, -713.07) Remaining time to fill dewars is 6900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 170 24 125 20, reference by 225 20 155 20 Disparity in stage position after aligning to target position: -0.24 -0.28 (2nd move error 0.06 -0.13) Finished aligning to item in 1 rounds Trimming A by 170 21 125 20, reference by 170 24 125 20 -16.9 -3.0 unbinned pixels; 11.1 65.3 nm along two shift axes; 15.2 -65.1 nm on specimen axes Total_Shift = 34.324793 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.11 microns changed by -3.41 to target drift = 0.10 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2654.tif 27 frames are being saved to U:\krios\20190717\PilA_2655.tif 27 frames are being saved to U:\krios\20190717\PilA_2656.tif 27 frames are being saved to U:\krios\20190717\PilA_2657.tif 27 frames are being saved to U:\krios\20190717\PilA_2658.tif 27 frames are being saved to U:\krios\20190717\PilA_2659.tif Running script 17 at item 513 (-179.28, -711.30) Remaining time to fill dewars is 6720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 190 20 110 20, reference by 225 20 155 20 Disparity in stage position after aligning to target position: -0.19 -0.40 (2nd move error 0.10 -0.25) Finished aligning to item in 1 rounds Trimming A by 185 24 115 20, reference by 190 20 110 20 -26.5 -1.5 unbinned pixels; 29.3 97.7 nm along two shift axes; 11.3 -102.9 nm on specimen axes Total_Shift = 53.144515 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.04 microns changed by -3.44 to target drift = 0.13 nm/sec Measured defocus = -1.35 microns changed by -0.05 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2660.tif 27 frames are being saved to U:\krios\20190717\PilA_2661.tif 27 frames are being saved to U:\krios\20190717\PilA_2662.tif 27 frames are being saved to U:\krios\20190717\PilA_2663.tif 27 frames are being saved to U:\krios\20190717\PilA_2664.tif 27 frames are being saved to U:\krios\20190717\PilA_2665.tif Running script 17 at item 514 (-183.42, -712.85) Remaining time to fill dewars is 6600 Dewars are NOT busy filling Trimming A by 220 22 20 190, reference by 225 20 20 190 Trimming A by 220 22 20 190, reference by 225 20 155 20 Trimming A by 220 22 20 190, reference by 20 105 20 130 Trimming A by 220 22 20 190, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.33 -0.11 Trimming A by 130 20 20 85, reference by 225 20 20 190 Disparity in stage position after aligning to target position: -0.23 0.08 (2nd move error 0.10 0.18) Finished aligning to item in 2 rounds Trimming A by 140 20 20 80, reference by 130 20 20 85 -0.4 -8.3 unbinned pixels; 29.9 14.0 nm along two shift axes; 32.3 0.1 nm on specimen axes Total_Shift = 16.575595 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.14 microns changed by -3.64 to target drift = 0.15 nm/sec Measured defocus = -1.56 microns changed by 0.06 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2666.tif 27 frames are being saved to U:\krios\20190717\PilA_2667.tif 27 frames are being saved to U:\krios\20190717\PilA_2668.tif 27 frames are being saved to U:\krios\20190717\PilA_2669.tif 27 frames are being saved to U:\krios\20190717\PilA_2670.tif 27 frames are being saved to U:\krios\20190717\PilA_2671.tif Running script 17 at item 515 (-180.91, -713.84) Remaining time to fill dewars is 6420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 105 22 65 20, reference by 225 20 155 20 Disparity in stage position after aligning to target position: -0.28 -0.52 (2nd move error 0.01 -0.36) Finished aligning to item in 1 rounds Trimming A by 100 20 65 20, reference by 105 22 65 20 -38.0 0.8 unbinned pixels; 52.6 135.6 nm along two shift axes; 4.8 -148.1 nm on specimen axes Total_Shift = 76.042924 Trimming A by 95 23 65 20, reference by 105 22 65 20 -5.2 -1.1 unbinned pixels; 2.9 20.4 nm along two shift axes; 5.3 -20.1 nm on specimen axes Total_Shift = 10.659982 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.59 microns changed by -2.19 to target drift = 0.12 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2672.tif 27 frames are being saved to U:\krios\20190717\PilA_2673.tif 27 frames are being saved to U:\krios\20190717\PilA_2674.tif 27 frames are being saved to U:\krios\20190717\PilA_2675.tif 27 frames are being saved to U:\krios\20190717\PilA_2676.tif 27 frames are being saved to U:\krios\20190717\PilA_2677.tif Running script 17 at item 516 (-178.45, -715.55) Remaining time to fill dewars is 6180 Dewars are NOT busy filling Trimming A by 235 24 160 20, reference by 225 20 20 190 Trimming A by 235 24 160 20, reference by 225 20 155 20 Trimming A by 235 24 160 20, reference by 20 105 20 130 Trimming A by 235 24 160 20, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.27 -0.15 Trimming A by 90 23 75 20, reference by 225 20 155 20 Disparity in stage position after aligning to target position: -0.25 -0.42 (2nd move error 0.01 -0.29) Finished aligning to item in 2 rounds Trimming A by 90 23 70 20, reference by 90 23 75 20 -30.5 0.5 unbinned pixels; 41.8 109.1 nm along two shift axes; 4.3 -118.9 nm on specimen axes Total_Shift = 61.063599 Trimming A by 85 24 70 20, reference by 90 23 75 20 -0.6 -1.3 unbinned pixels; 4.1 4.0 nm along two shift axes; 5.2 -1.9 nm on specimen axes Total_Shift = 2.858841 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.81 microns changed by -3.51 to target drift = 0.05 nm/sec Measured defocus = -1.74 microns changed by 0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2678.tif 27 frames are being saved to U:\krios\20190717\PilA_2679.tif 27 frames are being saved to U:\krios\20190717\PilA_2680.tif 27 frames are being saved to U:\krios\20190717\PilA_2681.tif 27 frames are being saved to U:\krios\20190717\PilA_2682.tif 27 frames are being saved to U:\krios\20190717\PilA_2683.tif Running script 17 at item 517 (-176.17, -717.27) Remaining time to fill dewars is 6060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 80 24 75 20, reference by 225 20 155 20 Disparity in stage position after aligning to target position: -0.16 -0.28 (2nd move error 0.10 -0.15) Finished aligning to item in 1 rounds Trimming A by 80 24 75 20, reference by 80 24 75 20 -16.5 -2.5 unbinned pixels; 12.4 63.1 nm along two shift axes; 13.1 -63.6 nm on specimen axes Total_Shift = 33.329031 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.30 microns changed by -2.10 to target drift = 0.15 nm/sec Measured defocus = -1.84 microns changed by 0.04 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2684.tif 27 frames are being saved to U:\krios\20190717\PilA_2685.tif 27 frames are being saved to U:\krios\20190717\PilA_2686.tif 27 frames are being saved to U:\krios\20190717\PilA_2687.tif 27 frames are being saved to U:\krios\20190717\PilA_2688.tif 27 frames are being saved to U:\krios\20190717\PilA_2689.tif Running script 17 at item 518 (-173.27, -718.93) Remaining time to fill dewars is 5820 Dewars are NOT busy filling Trimming A by 23 71 135 20, reference by 225 20 20 190 Trimming A by 23 71 135 20, reference by 225 20 155 20 Trimming A by 23 71 135 20, reference by 20 105 20 130 Trimming A by 23 71 135 20, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.24 Trimming A by 70 31 105 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.32 -0.11 (2nd move error -0.10 0.11) Finished aligning to item in 2 rounds Trimming A by 75 25 105 20, reference by 70 31 105 20 0.2 20.8 unbinned pixels; 76.2 32.1 nm along two shift axes; -81.0 -3.5 nm on specimen axes Total_Shift = 41.602054 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.46 microns changed by -3.36 to target drift = 0.16 nm/sec Measured defocus = -1.90 microns changed by 0.00 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2690.tif 27 frames are being saved to U:\krios\20190717\PilA_2691.tif 27 frames are being saved to U:\krios\20190717\PilA_2692.tif 27 frames are being saved to U:\krios\20190717\PilA_2693.tif 27 frames are being saved to U:\krios\20190717\PilA_2694.tif 27 frames are being saved to U:\krios\20190717\PilA_2695.tif Running script 17 at item 519 (-170.80, -720.75) Remaining time to fill dewars is 5580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 100 170 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.39 -0.03 (2nd move error -0.17 0.19) Finished aligning to item in 1 rounds Trimming A by 24 67 190 20, reference by 21 100 170 20 5.2 32.9 unbinned pixels; 114.1 68.1 nm along two shift axes; -129.0 13.2 nm on specimen axes Total_Shift = 66.553842 Trimming A by 21 62 190 20, reference by 21 100 170 20 2.1 19.0 unbinned pixels; 67.1 36.1 nm along two shift axes; -74.3 4.1 nm on specimen axes Total_Shift = 38.210447 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Using peak at -6.0,-1.6, rejecting 1.19 times higher peak at 0.0,-0.3 Measured defocus = -0.08 microns changed by -1.92 to target drift = 0.12 nm/sec Measured defocus = -1.96 microns changed by -0.04 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2696.tif 27 frames are being saved to U:\krios\20190717\PilA_2697.tif 27 frames are being saved to U:\krios\20190717\PilA_2698.tif 27 frames are being saved to U:\krios\20190717\PilA_2699.tif 27 frames are being saved to U:\krios\20190717\PilA_2700.tif 27 frames are being saved to U:\krios\20190717\PilA_2701.tif Running script 17 at item 520 (-172.44, -723.37) Remaining time to fill dewars is 5460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 101 160 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.26 -0.16 (2nd move error -0.03 0.06) Finished aligning to item in 1 rounds Trimming A by 24 105 155 20, reference by 22 101 160 20 -1.5 6.8 unbinned pixels; 27.0 4.8 nm along two shift axes; -26.1 -7.3 nm on specimen axes Total_Shift = 13.92298 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.32 microns changed by -3.42 to target drift = 0.10 nm/sec Measured defocus = -2.16 microns changed by 0.06 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2702.tif 27 frames are being saved to U:\krios\20190717\PilA_2703.tif 27 frames are being saved to U:\krios\20190717\PilA_2704.tif 27 frames are being saved to U:\krios\20190717\PilA_2705.tif 27 frames are being saved to U:\krios\20190717\PilA_2706.tif 27 frames are being saved to U:\krios\20190717\PilA_2707.tif Running script 17 at item 521 (-174.96, -721.59) Remaining time to fill dewars is 5220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 41 80 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.28 -0.32 (2nd move error -0.06 -0.09) Finished aligning to item in 1 rounds Trimming A by 21 41 80 20, reference by 21 41 80 20 -10.4 11.2 unbinned pixels; 54.9 20.5 nm along two shift axes; -41.5 -42.8 nm on specimen axes Total_Shift = 30.61431 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.05 microns changed by -2.15 to target drift = 0.03 nm/sec Measured defocus = -2.23 microns changed by 0.03 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2708.tif 27 frames are being saved to U:\krios\20190717\PilA_2709.tif 27 frames are being saved to U:\krios\20190717\PilA_2710.tif 27 frames are being saved to U:\krios\20190717\PilA_2711.tif 27 frames are being saved to U:\krios\20190717\PilA_2712.tif 27 frames are being saved to U:\krios\20190717\PilA_2713.tif Ending Navigator acquisition after current item Running script 17 at item 522 (-177.46, -719.77) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Running script 17 at item 522 (-177.46, -719.77) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.34 -0.42 (2nd move error -0.13 -0.19) Finished aligning to item in 1 rounds -20.2 28.0 unbinned pixels; 129.5 30.5 nm along two shift axes; -104.9 -84.5 nm on specimen axes Total_Shift = 69.120117 -1.4 18.3 unbinned pixels; 69.0 22.4 nm along two shift axes; -70.8 -9.4 nm on specimen axes Total_Shift = 36.645169 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.17 microns changed by -3.47 to target drift = 0.04 nm/sec Measured defocus = -2.34 microns changed by 0.04 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2714.tif 27 frames are being saved to U:\krios\20190717\PilA_2715.tif 27 frames are being saved to U:\krios\20190717\PilA_2716.tif 27 frames are being saved to U:\krios\20190717\PilA_2717.tif 27 frames are being saved to U:\krios\20190717\PilA_2718.tif 27 frames are being saved to U:\krios\20190717\PilA_2719.tif Running script 17 at item 523 (-180.56, -717.86) Remaining time to fill dewars is 4860 Dewars are NOT busy filling Trimming A by 235 24 160 20, reference by 225 20 20 190 Trimming A by 235 24 160 20, reference by 225 20 155 20 Trimming A by 235 24 160 20, reference by 20 105 20 130 Trimming A by 235 24 160 20, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.22 -0.20 Trimming A by 20 20 20 20, reference by 225 20 155 20 Disparity in stage position after aligning to target position: -0.19 -0.54 (2nd move error 0.03 -0.36) Finished aligning to item in 2 rounds -31.2 -2.8 unbinned pixels; 30.6 116.4 nm along two shift axes; 17.3 -120.8 nm on specimen axes Total_Shift = 62.622534 -5.2 -2.8 unbinned pixels; 3.5 22.8 nm along two shift axes; 11.9 -19.5 nm on specimen axes Total_Shift = 11.741272 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.15 microns changed by -2.25 to target drift = 0.18 nm/sec Measured defocus = -2.41 microns changed by 0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2720.tif 27 frames are being saved to U:\krios\20190717\PilA_2721.tif 27 frames are being saved to U:\krios\20190717\PilA_2722.tif 27 frames are being saved to U:\krios\20190717\PilA_2723.tif 27 frames are being saved to U:\krios\20190717\PilA_2724.tif 27 frames are being saved to U:\krios\20190717\PilA_2725.tif Running script 17 at item 524 (-185.10, -715.28) Remaining time to fill dewars is 4620 Dewars are NOT busy filling Trimming A by 220 22 20 190, reference by 225 20 20 190 Trimming A by 220 22 20 190, reference by 225 20 155 20 Trimming A by 220 22 20 190, reference by 20 105 20 130 Trimming A by 220 22 20 190, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.34 -0.14 Trimming A by 110 23 20 100, reference by 225 20 20 190 Disparity in stage position after aligning to target position: -0.11 -0.01 (2nd move error 0.22 0.12) Finished aligning to item in 2 rounds Trimming A by 110 23 20 95, reference by 110 23 20 100 2.8 4.7 unbinned pixels; 13.7 17.2 nm along two shift axes; -18.9 9.9 nm on specimen axes Total_Shift = 10.964394 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.21 microns changed by -3.71 to target drift = 0.10 nm/sec Measured defocus = -2.56 microns changed by 0.06 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2726.tif 27 frames are being saved to U:\krios\20190717\PilA_2727.tif 27 frames are being saved to U:\krios\20190717\PilA_2728.tif 27 frames are being saved to U:\krios\20190717\PilA_2729.tif 27 frames are being saved to U:\krios\20190717\PilA_2730.tif 27 frames are being saved to U:\krios\20190717\PilA_2731.tif Running script 17 at item 525 (-186.70, -717.90) Remaining time to fill dewars is 4440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 61 21 20 135, reference by 225 20 20 190 Disparity in stage position after aligning to target position: -0.13 -0.12 (2nd move error 0.21 -0.00) Finished aligning to item in 1 rounds Trimming A by 64 24 20 125, reference by 61 21 20 135 1.1 -1.7 unbinned pixels; 7.6 1.2 nm along two shift axes; 6.4 4.5 nm on specimen axes Total_Shift = 4.011397 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.12 microns changed by -2.48 to target drift = 0.11 nm/sec Measured defocus = -2.62 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2732.tif 27 frames are being saved to U:\krios\20190717\PilA_2733.tif 27 frames are being saved to U:\krios\20190717\PilA_2734.tif 27 frames are being saved to U:\krios\20190717\PilA_2735.tif 27 frames are being saved to U:\krios\20190717\PilA_2736.tif 27 frames are being saved to U:\krios\20190717\PilA_2737.tif Running script 17 at item 526 (-184.00, -719.55) Remaining time to fill dewars is 4320 Dewars are NOT busy filling Trimming A by 21 105 50 115, reference by 225 20 20 190 Trimming A by 21 105 50 115, reference by 225 20 155 20 Trimming A by 21 105 50 115, reference by 20 105 20 130 Trimming A by 21 105 50 115, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.17 Trimming A by 30 20 20 30, reference by 20 105 20 130 Disparity in stage position after aligning to target position: -0.34 0.10 (2nd move error -0.04 0.25) Finished aligning to item in 2 rounds Trimming A by 32 20 20 30, reference by 30 20 20 30 -8.3 27.4 unbinned pixels; 111.6 11.2 nm along two shift axes; -104.8 -38.2 nm on specimen axes Total_Shift = 57.248639 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.34 microns changed by -2.44 to target drift = 0.06 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2738.tif 27 frames are being saved to U:\krios\20190717\PilA_2739.tif 27 frames are being saved to U:\krios\20190717\PilA_2740.tif 27 frames are being saved to U:\krios\20190717\PilA_2741.tif 27 frames are being saved to U:\krios\20190717\PilA_2742.tif 27 frames are being saved to U:\krios\20190717\PilA_2743.tif Running script 17 at item 527 (-181.99, -720.29) Remaining time to fill dewars is 4140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 105 20 130 Disparity in stage position after aligning to target position: -0.23 0.12 (2nd move error 0.08 0.28) Finished aligning to item in 1 rounds -1.7 3.6 unbinned pixels; 15.3 0.6 nm along two shift axes; -13.5 -7.2 nm on specimen axes Total_Shift = 7.862277 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.48 microns changed by -3.68 to target drift = 0.09 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2744.tif 27 frames are being saved to U:\krios\20190717\PilA_2745.tif 27 frames are being saved to U:\krios\20190717\PilA_2746.tif 27 frames are being saved to U:\krios\20190717\PilA_2747.tif 27 frames are being saved to U:\krios\20190717\PilA_2748.tif 27 frames are being saved to U:\krios\20190717\PilA_2749.tif Running script 17 at item 528 (-179.09, -722.37) Remaining time to fill dewars is 3900 Dewars are NOT busy filling Trimming A by 20 100 120 20, reference by 225 20 20 190 Trimming A by 20 100 120 20, reference by 225 20 155 20 Trimming A by 20 100 120 20, reference by 20 105 20 130 Trimming A by 20 100 120 20, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.27 -0.16 Trimming A by 20 20 20 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.26 -0.50 (2nd move error 0.00 -0.36) Finished aligning to item in 2 rounds -36.2 2.8 unbinned pixels; 57.5 126.0 nm along two shift axes; -3.2 -141.3 nm on specimen axes Total_Shift = 72.559626 -4.5 0.2 unbinned pixels; 6.7 16.0 nm along two shift axes; 0.1 -17.7 nm on specimen axes Total_Shift = 9.098349 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.35 microns changed by -3.65 to target drift = 0.07 nm/sec Measured defocus = -1.36 microns changed by 0.06 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2750.tif 27 frames are being saved to U:\krios\20190717\PilA_2751.tif 27 frames are being saved to U:\krios\20190717\PilA_2752.tif 27 frames are being saved to U:\krios\20190717\PilA_2753.tif 27 frames are being saved to U:\krios\20190717\PilA_2754.tif 27 frames are being saved to U:\krios\20190717\PilA_2755.tif Running script 17 at item 529 (-176.60, -724.15) Remaining time to fill dewars is 3720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 47 75 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.24 -0.36 (2nd move error 0.03 -0.22) Finished aligning to item in 1 rounds Trimming A by 21 46 75 20, reference by 22 47 75 20 -25.9 -1.5 unbinned pixels; 28.4 95.5 nm along two shift axes; 11.2 -100.5 nm on specimen axes Total_Shift = 51.901497 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.14 microns changed by -3.54 to target drift = 0.12 nm/sec Measured defocus = -1.43 microns changed by 0.03 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2756.tif 27 frames are being saved to U:\krios\20190717\PilA_2757.tif 27 frames are being saved to U:\krios\20190717\PilA_2758.tif 27 frames are being saved to U:\krios\20190717\PilA_2759.tif 27 frames are being saved to U:\krios\20190717\PilA_2760.tif 27 frames are being saved to U:\krios\20190717\PilA_2761.tif Running script 17 at item 530 (-174.18, -725.89) Remaining time to fill dewars is 3600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 104 150 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.23 -0.18 (2nd move error 0.03 -0.04) Finished aligning to item in 1 rounds Trimming A by 20 107 150 20, reference by 23 104 150 20 -5.2 -1.2 unbinned pixels; 2.5 20.5 nm along two shift axes; 5.6 -20.0 nm on specimen axes Total_Shift = 10.654127 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.69 microns changed by -2.19 to target drift = 0.15 nm/sec Measured defocus = -1.50 microns changed by 0.00 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2762.tif 27 frames are being saved to U:\krios\20190717\PilA_2763.tif 27 frames are being saved to U:\krios\20190717\PilA_2764.tif 27 frames are being saved to U:\krios\20190717\PilA_2765.tif 27 frames are being saved to U:\krios\20190717\PilA_2766.tif 27 frames are being saved to U:\krios\20190717\PilA_2767.tif Running script 17 at item 531 (-175.86, -728.35) Remaining time to fill dewars is 3360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 135 115 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.16 -0.28 (2nd move error 0.10 -0.15) Finished aligning to item in 1 rounds Trimming A by 24 140 115 20, reference by 20 135 115 20 -14.1 -1.3 unbinned pixels; 13.8 52.8 nm along two shift axes; 7.9 -54.7 nm on specimen axes Total_Shift = 28.3689 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.04 microns changed by -3.64 to target drift = 0.08 nm/sec Measured defocus = -1.63 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2768.tif 27 frames are being saved to U:\krios\20190717\PilA_2769.tif 27 frames are being saved to U:\krios\20190717\PilA_2770.tif 27 frames are being saved to U:\krios\20190717\PilA_2771.tif 27 frames are being saved to U:\krios\20190717\PilA_2772.tif 27 frames are being saved to U:\krios\20190717\PilA_2773.tif Running script 17 at item 532 (-178.29, -726.68) Remaining time to fill dewars is 3180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 60 60 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.23 -0.46 (2nd move error 0.03 -0.34) Finished aligning to item in 1 rounds Trimming A by 24 75 55 20, reference by 21 60 60 20 -32.8 -2.9 unbinned pixels; 32.2 122.6 nm along two shift axes; 18.2 -127.1 nm on specimen axes Total_Shift = 65.922799 Trimming A by 20 70 60 20, reference by 21 60 60 20 -3.7 -2.9 unbinned pixels; 5.7 17.5 nm along two shift axes; 11.9 -13.7 nm on specimen axes Total_Shift = 9.300619 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.66 microns changed by -2.36 to target drift = 0.20 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2774.tif 27 frames are being saved to U:\krios\20190717\PilA_2775.tif 27 frames are being saved to U:\krios\20190717\PilA_2776.tif 27 frames are being saved to U:\krios\20190717\PilA_2777.tif 27 frames are being saved to U:\krios\20190717\PilA_2778.tif 27 frames are being saved to U:\krios\20190717\PilA_2779.tif Running script 17 at item 533 (-180.75, -724.92) Remaining time to fill dewars is 3000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 31 20 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.26 -0.59 (2nd move error -0.00 -0.45) Finished aligning to item in 1 rounds Trimming A by 20 26 20 35, reference by 20 31 20 20 -37.8 2.7 unbinned pixels; 59.3 132.1 nm along two shift axes; -2.5 -147.7 nm on specimen axes Total_Shift = 75.843059 Trimming A by 20 30 20 35, reference by 20 31 20 20 -0.4 -0.3 unbinned pixels; 0.4 2.0 nm along two shift axes; 1.2 -1.7 nm on specimen axes Total_Shift = 1.040118 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 2.01 microns changed by -3.81 to target drift = 0.08 nm/sec Measured defocus = -1.87 microns changed by 0.07 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2780.tif 27 frames are being saved to U:\krios\20190717\PilA_2781.tif 27 frames are being saved to U:\krios\20190717\PilA_2782.tif 27 frames are being saved to U:\krios\20190717\PilA_2783.tif 27 frames are being saved to U:\krios\20190717\PilA_2784.tif 27 frames are being saved to U:\krios\20190717\PilA_2785.tif Running script 17 at item 534 (-183.19, -723.89) Remaining time to fill dewars is 2760 Dewars are NOT busy filling Trimming A by 21 105 50 115, reference by 225 20 20 190 Trimming A by 21 105 50 115, reference by 225 20 155 20 Trimming A by 21 105 50 115, reference by 20 105 20 130 Trimming A by 21 105 50 115, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.18 Trimming A by 21 35 20 45, reference by 20 105 20 130 Disparity in stage position after aligning to target position: -0.28 0.12 (2nd move error 0.07 0.28) Finished aligning to item in 2 rounds Trimming A by 20 20 20 45, reference by 21 35 20 45 0.4 -0.7 unbinned pixels; 3.2 0.3 nm along two shift axes; 2.7 1.6 nm on specimen axes Total_Shift = 1.637494 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.52 microns changed by -2.42 to target drift = 0.12 nm/sec Measured defocus = -1.83 microns changed by -0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2786.tif 27 frames are being saved to U:\krios\20190717\PilA_2787.tif 27 frames are being saved to U:\krios\20190717\PilA_2788.tif 27 frames are being saved to U:\krios\20190717\PilA_2789.tif 27 frames are being saved to U:\krios\20190717\PilA_2790.tif 27 frames are being saved to U:\krios\20190717\PilA_2791.tif Running script 17 at item 535 (-185.66, -722.11) Remaining time to fill dewars is 2700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 25 20 45, reference by 20 105 20 130 Disparity in stage position after aligning to target position: -0.26 -0.10 (2nd move error 0.09 0.06) Finished aligning to item in 1 rounds Trimming A by 30 30 20 40, reference by 21 25 20 45 -0.9 -3.6 unbinned pixels; 11.9 8.7 nm along two shift axes; 14.1 -2.8 nm on specimen axes Total_Shift = 7.366446 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.90 microns changed by -3.90 to target drift = 0.09 nm/sec Measured defocus = -2.06 microns changed by 0.06 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2792.tif 27 frames are being saved to U:\krios\20190717\PilA_2793.tif 27 frames are being saved to U:\krios\20190717\PilA_2794.tif 27 frames are being saved to U:\krios\20190717\PilA_2795.tif 27 frames are being saved to U:\krios\20190717\PilA_2796.tif 27 frames are being saved to U:\krios\20190717\PilA_2797.tif Running script 17 at item 536 (-188.14, -720.31) Remaining time to fill dewars is 2460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 57 22 20 135, reference by 20 105 20 130 Disparity in stage position after aligning to target position: -0.34 -0.20 (2nd move error -0.00 -0.04) Finished aligning to item in 1 rounds Trimming A by 61 23 20 130, reference by 57 22 20 135 -4.7 1.9 unbinned pixels; 13.1 14.0 nm along two shift axes; -6.4 -18.6 nm on specimen axes Total_Shift = 10.097116 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.49 microns changed by -2.59 to target drift = 0.07 nm/sec Measured defocus = -2.13 microns changed by 0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2798.tif 27 frames are being saved to U:\krios\20190717\PilA_2799.tif 27 frames are being saved to U:\krios\20190717\PilA_2800.tif 27 frames are being saved to U:\krios\20190717\PilA_2801.tif 27 frames are being saved to U:\krios\20190717\PilA_2802.tif 27 frames are being saved to U:\krios\20190717\PilA_2803.tif Running script 17 at item 537 (-187.33, -724.69) Remaining time to fill dewars is 2340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 85 20 120, reference by 20 105 20 130 Disparity in stage position after aligning to target position: -0.26 -0.17 (2nd move error 0.09 -0.02) Finished aligning to item in 1 rounds Trimming A by 20 90 20 115, reference by 22 85 20 120 -2.9 -2.7 unbinned pixels; 6.1 14.5 nm along two shift axes; 11.1 -10.8 nm on specimen axes Total_Shift = 7.922991 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.69 microns changed by -3.89 to target drift = 0.12 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2804.tif 27 frames are being saved to U:\krios\20190717\PilA_2805.tif 27 frames are being saved to U:\krios\20190717\PilA_2806.tif 27 frames are being saved to U:\krios\20190717\PilA_2807.tif 27 frames are being saved to U:\krios\20190717\PilA_2808.tif 27 frames are being saved to U:\krios\20190717\PilA_2809.tif Running script 17 at item 538 (-184.88, -726.43) Remaining time to fill dewars is 2040 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 24 100 20 110, reference by 20 105 20 130 Disparity in stage position after aligning to target position: -0.18 -0.04 (2nd move error 0.17 0.12) Finished aligning to item in 1 rounds Trimming A by 24 95 20 105, reference by 24 100 20 110 2.6 3.8 unbinned pixels; 10.7 15.0 nm along two shift axes; -15.5 9.2 nm on specimen axes Total_Shift = 9.235931 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.22 microns changed by -2.52 to target drift = 0.07 nm/sec Measured defocus = -2.30 microns changed by -0.00 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2810.tif 27 frames are being saved to U:\krios\20190717\PilA_2811.tif 27 frames are being saved to U:\krios\20190717\PilA_2812.tif 27 frames are being saved to U:\krios\20190717\PilA_2813.tif 27 frames are being saved to U:\krios\20190717\PilA_2814.tif 27 frames are being saved to U:\krios\20190717\PilA_2815.tif Running script 17 at item 539 (-182.43, -728.20) Remaining time to fill dewars is 1920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 110 20 105, reference by 20 105 20 130 Disparity in stage position after aligning to target position: -0.07 0.14 (2nd move error 0.27 0.30) Finished aligning to item in 1 rounds Trimming A by 23 105 20 95, reference by 23 110 20 105 4.7 -3.4 unbinned pixels; 18.6 11.9 nm along two shift axes; 12.1 19.1 nm on specimen axes Total_Shift = 11.61069 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.31 microns changed by -3.71 to target drift = 0.12 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2816.tif 27 frames are being saved to U:\krios\20190717\PilA_2817.tif 27 frames are being saved to U:\krios\20190717\PilA_2818.tif 27 frames are being saved to U:\krios\20190717\PilA_2819.tif 27 frames are being saved to U:\krios\20190717\PilA_2820.tif 27 frames are being saved to U:\krios\20190717\PilA_2821.tif Running script 17 at item 540 (-179.92, -729.17) Remaining time to fill dewars is 1860 Dewars are NOT busy filling Trimming A by 20 75 135 20, reference by 225 20 20 190 Trimming A by 20 75 135 20, reference by 225 20 155 20 Trimming A by 20 75 135 20, reference by 20 105 20 130 Trimming A by 20 75 135 20, reference by 22 78 125 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.17 Trimming A by 23 115 20 85, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.14 -0.48 (2nd move error 0.11 -0.33) Finished aligning to item in 2 rounds Trimming A by 23 120 25 85, reference by 23 115 20 85 -30.9 -6.7 unbinned pixels; 15.7 121.4 nm along two shift axes; 32.6 -118.8 nm on specimen axes Total_Shift = 63.259841 Trimming A by 22 125 20 85, reference by 23 115 20 85 -2.8 -2.3 unbinned pixels; 4.7 13.5 nm along two shift axes; 9.4 -10.4 nm on specimen axes Total_Shift = 7.193957 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.13 microns changed by -2.37 to target drift = 0.16 nm/sec Measured defocus = -2.49 microns changed by -0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2822.tif 27 frames are being saved to U:\krios\20190717\PilA_2823.tif 27 frames are being saved to U:\krios\20190717\PilA_2824.tif 27 frames are being saved to U:\krios\20190717\PilA_2825.tif Ending Navigator acquisition after current item 27 frames are being saved to U:\krios\20190717\PilA_2826.tif 27 frames are being saved to U:\krios\20190717\PilA_2827.tif Running script 17 at item 541 (-177.42, -730.86) Remaining time to fill dewars is 1440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 175 100 20, reference by 22 78 125 20 Disparity in stage position after aligning to target position: -0.10 -0.37 (2nd move error 0.14 -0.22) Finished aligning to item in 1 rounds Trimming A by 20 170 105 20, reference by 22 175 100 20 -22.5 -1.9 unbinned pixels; 22.5 83.7 nm along two shift axes; 11.9 -87.0 nm on specimen axes Total_Shift = 45.082044 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 1.09 microns changed by -3.69 to target drift = 0.18 nm/sec Measured defocus = -2.72 microns changed by 0.12 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2828.tif 27 frames are being saved to U:\krios\20190717\PilA_2829.tif 27 frames are being saved to U:\krios\20190717\PilA_2830.tif 27 frames are being saved to U:\krios\20190717\PilA_2831.tif 27 frames are being saved to U:\krios\20190717\PilA_2832.tif 27 frames are being saved to U:\krios\20190717\PilA_2833.tif EDIT MODE REMINDER: Left-click to select Ctrl-Left-Click to add or remove from selected points Left-double-click to delete point if already selected Middle-click to add a point Right-click to move current point Backspace to delete current point Running script 6 at item 240 (-15.15, -153.79) Trimming A by 55 55, reference by 50 50 Trimming A by 60 65, reference by 50 50 Trimming A by 85 85, reference by 50 50 Rough eucentricity: changing Z by 20.22 to 95.46, continuing... Trimming A by 90 95, reference by 95 95 Trimming A by 90 90, reference by 95 95 Rough eucentricity: changing Z by -1.58 to 93.88, finished. Z = 0: The original displacements in the overlap zones have mean 12.10 maximum 20.51 pixels. After shifting pieces into register, the displacements have mean 7.52 maximum 7.52 pixels. Running script 6 at item 241 (67.60, -131.73) Trimming A by 25 30, reference by 20 20 Rough eucentricity: changing Z by 20.12 to 95.36, continuing... Trimming A by 35 35, reference by 35 40 Trimming A by 30 35, reference by 35 40 Rough eucentricity: changing Z by -1.77 to 93.59, finished. Z = 1: The original displacements in the overlap zones have mean 24.13 maximum 29.91 pixels. After shifting pieces into register, the displacements have mean 5.61 maximum 5.61 pixels. Running script 6 at item 242 (571.70, -493.78) Rough eucentricity: changing Z by 3.16 to 78.40, finished. Z = 2: The original displacements in the overlap zones have mean 23.21 maximum 38.27 pixels. After shifting pieces into register, the displacements have mean 2.98 maximum 2.98 pixels. Running script 6 at item 243 (526.14, -319.97) Trimming A by 20 25, reference by 20 20 Trimming A by 30 35, reference by 20 20 Trimming A by 35 40, reference by 20 20 Rough eucentricity: changing Z by 9.01 to 84.25, finished. Z = 3: The original displacements in the overlap zones have mean 21.99 maximum 33.32 pixels. After shifting pieces into register, the displacements have mean 0.25 maximum 0.25 pixels. Running script 6 at item 244 (419.85, -257.30) Trimming A by 60 60, reference by 55 60 Trimming A by 65 65, reference by 55 60 Trimming A by 80 80, reference by 55 60 Trimming A by 90 95, reference by 55 60 Rough eucentricity: changing Z by 12.57 to 87.81, finished. Z = 4: The original displacements in the overlap zones have mean 18.27 maximum 37.53 pixels. After shifting pieces into register, the displacements have mean 8.38 maximum 8.38 pixels. Running script 6 at item 245 (394.98, -171.56) Trimming A by 40 40, reference by 35 35 Trimming A by 45 45, reference by 35 35 Trimming A by 60 60, reference by 35 35 Trimming A by 70 70, reference by 35 35 Rough eucentricity: changing Z by 13.26 to 88.49, finished. Z = 5: The original displacements in the overlap zones have mean 21.92 maximum 34.63 pixels. After shifting pieces into register, the displacements have mean 7.13 maximum 7.13 pixels. Running script 6 at item 246 (340.96, -143.01) Trimming A by 35 35, reference by 40 40 Trimming A by 30 30, reference by 40 40 Trimming A by 20 20, reference by 40 40 Trimming A by 25 30, reference by 40 40 Rough eucentricity: changing Z by 15.61 to 90.84, finished. Z = 6: The original displacements in the overlap zones have mean 26.71 maximum 50.52 pixels. After shifting pieces into register, the displacements have mean 15.60 maximum 15.60 pixels. Running script 6 at item 247 (-703.25, -131.47) Rough eucentricity: changing Z by 2.56 to 77.79, finished. Z = 7: The original displacements in the overlap zones have mean 37.21 maximum 71.77 pixels. After shifting pieces into register, the displacements have mean 15.03 maximum 15.03 pixels. Running script 6 at item 720 (366.83, -746.56) Trimming A by 125 125, reference by 125 125 Trimming A by 125 125, reference by 125 125 Trimming A by 120 120, reference by 125 125 Trimming A by 115 115, reference by 125 125 Rough eucentricity: changing Z by 3.49 to 78.73, finished. Z = 8: The original displacements in the overlap zones have mean 11.32 maximum 23.14 pixels. After shifting pieces into register, the displacements have mean 3.49 maximum 3.49 pixels. Running script 6 at item 721 (-412.51, -880.35) Rough eucentricity: changing Z by -9.54 to 65.69, finished. Z = 9: The original displacements in the overlap zones have mean 30.76 maximum 49.08 pixels. After shifting pieces into register, the displacements have mean 1.35 maximum 1.35 pixels. Running script 6 at item 722 (-771.87, -385.01) Trimming A by 85 90, reference by 85 90 Trimming A by 85 85, reference by 85 90 Trimming A by 75 80, reference by 85 90 Trimming A by 52 90, reference by 85 90 Rough eucentricity: changing Z by -6.65 to 68.56, finished. Z = 10: The original displacements in the overlap zones have mean 17.06 maximum 33.53 pixels. After shifting pieces into register, the displacements have mean 4.32 maximum 4.32 pixels. Running script 6 at item 723 (-536.32, -80.27) Rough eucentricity: changing Z by 9.24 to 84.46, finished. Z = 11: The original displacements in the overlap zones have mean 14.64 maximum 37.02 pixels. After shifting pieces into register, the displacements have mean 4.85 maximum 4.85 pixels. Ending Navigator acquisition after current item Running script 6 at item 724 (-896.19, -96.86) Trimming A by 40 40, reference by 40 40 Trimming A by 35 40, reference by 40 40 Trimming A by 30 30, reference by 40 40 Trimming A by 35 35, reference by 40 40 Rough eucentricity: changing Z by -6.05 to 69.19, finished. Z = 12: The original displacements in the overlap zones have mean 16.95 maximum 29.14 pixels. After shifting pieces into register, the displacements have mean 4.03 maximum 4.03 pixels. Running script 6 at item 725 (-507.19, -23.81) Trimming A by 20 25, reference by 20 25 Trimming A by 25 25, reference by 20 25 Trimming A by 25 40, reference by 20 20 Trimming A by 40 40, reference by 20 25 Rough eucentricity: changing Z by 10.25 to 85.47, finished. Z = 13: The original displacements in the overlap zones have mean 18.53 maximum 30.37 pixels. After shifting pieces into register, the displacements have mean 10.74 maximum 10.74 pixels. Running script 6 at item 726 (-231.67, -34.59) Rough eucentricity: changing Z by 16.98 to 92.21, continuing... Rough eucentricity: changing Z by -1.53 to 90.68, finished. Z = 14: The original displacements in the overlap zones have mean 20.14 maximum 26.73 pixels. After shifting pieces into register, the displacements have mean 3.88 maximum 3.88 pixels. Running script 6 at item 727 (71.77, 10.83) Trimming A by 21 55, reference by 21 50 Trimming A by 35 55, reference by 21 50 Trimming A by 51 75, reference by 21 50 Rough eucentricity: changing Z by 18.10 to 93.34, continuing... Trimming A by 70 70, reference by 75 75 Trimming A by 70 70, reference by 75 75 Rough eucentricity: changing Z by -1.67 to 91.67, finished. Z = 15: The original displacements in the overlap zones have mean 19.29 maximum 24.99 pixels. After shifting pieces into register, the displacements have mean 0.55 maximum 0.55 pixels. Running script 6 at item 728 (344.31, -656.25) Rough eucentricity: changing Z by 9.11 to 84.35, finished. Z = 16: The original displacements in the overlap zones have mean 25.45 maximum 46.11 pixels. After shifting pieces into register, the displacements have mean 6.68 maximum 6.68 pixels. Running script 6 at item 729 (-783.01, -15.13) Trimming A by 35 40, reference by 35 40 Trimming A by 35 40, reference by 35 40 Trimming A by 35 35, reference by 35 40 Trimming A by 35 35, reference by 35 40 Rough eucentricity: changing Z by -0.59 to 74.65, finished. Z = 17: The original displacements in the overlap zones have mean 15.13 maximum 26.88 pixels. After shifting pieces into register, the displacements have mean 4.92 maximum 4.92 pixels. Running script 6 at item 858 (-70.37, -125.73) Trimming A by 32 65, reference by 32 70 Trimming A by 45 45, reference by 32 70 Trimming A by 60 60, reference by 32 70 Rough eucentricity: changing Z by 19.61 to 94.82, continuing... Trimming A by 70 70, reference by 70 75 Trimming A by 65 70, reference by 70 75 Rough eucentricity: changing Z by -1.73 to 93.09, finished. Z = 0: The original displacements in the overlap zones have mean 8.42 maximum 17.85 pixels. After shifting pieces into register, the displacements have mean 5.55 maximum 5.55 pixels. Running script 6 at item 859 (-202.18, 21.70) Trimming A by 25 30, reference by 25 30 Trimming A by 30 35, reference by 25 30 Trimming A by 50 50, reference by 25 30 Trimming A by 55 60, reference by 25 30 Rough eucentricity: changing Z by 15.58 to 90.81, finished. Z = 1: The original displacements in the overlap zones have mean 16.77 maximum 30.01 pixels. After shifting pieces into register, the displacements have mean 6.21 maximum 6.21 pixels. Running script 6 at item 860 (412.27, -399.46) Rough eucentricity: changing Z by 12.66 to 87.89, finished. Z = 2: The original displacements in the overlap zones have mean 29.15 maximum 44.53 pixels. After shifting pieces into register, the displacements have mean 2.88 maximum 2.88 pixels. Running script 6 at item 861 (-98.03, -695.78) Trimming A by 70 70, reference by 75 75 Trimming A by 60 60, reference by 75 75 Trimming A by 35 45, reference by 75 75 Rough eucentricity: changing Z by 18.41 to 93.65, continuing... Trimming A by 45 55, reference by 50 60 Trimming A by 40 55, reference by 50 60 Rough eucentricity: changing Z by -1.60 to 92.05, finished. Z = 3: The original displacements in the overlap zones have mean 4.48 maximum 6.67 pixels. After shifting pieces into register, the displacements have mean 1.00 maximum 1.00 pixels. Running script 6 at item 862 (-590.14, -48.85) Trimming A by 25 30, reference by 20 20 Trimming A by 30 35, reference by 20 20 Rough eucentricity: changing Z by 6.96 to 82.19, finished. Z = 4: The original displacements in the overlap zones have mean 13.57 maximum 25.38 pixels. After shifting pieces into register, the displacements have mean 10.86 maximum 10.86 pixels. Running script 6 at item 863 (-645.60, -19.17) Trimming A by 45 50, reference by 36 60 Trimming A by 45 55, reference by 36 60 Trimming A by 45 55, reference by 36 60 Trimming A by 50 60, reference by 36 60 Rough eucentricity: changing Z by 4.98 to 80.22, finished. Z = 5: The original displacements in the overlap zones have mean 14.86 maximum 22.46 pixels. After shifting pieces into register, the displacements have mean 1.85 maximum 1.85 pixels. Running script 6 at item 864 (-675.32, -75.95) Rough eucentricity: changing Z by 3.91 to 79.14, finished. Z = 6: The original displacements in the overlap zones have mean 17.64 maximum 29.50 pixels. After shifting pieces into register, the displacements have mean 1.79 maximum 1.79 pixels. Item 865 is being skipped because it is outside the limits for stage movement Running script 6 at item 866 (-381.70, -313.01) Rough eucentricity: changing Z by 14.49 to 89.73, finished. Z = 7: The original displacements in the overlap zones have mean 24.00 maximum 37.07 pixels. After shifting pieces into register, the displacements have mean 0.23 maximum 0.23 pixels. Running script 17 at item 542 (-248.47, -597.47) Remaining time to fill dewars is 30300 Dewars are NOT busy filling Trimming A by 135 24 120 20, reference by 139 23 20 195 Trimming A by 135 24 120 20, reference by 125 24 110 20 Trimming A by 135 24 120 20, reference by 21 160 20 165 Trimming A by 135 24 120 20, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.22 -0.36 Trimming A by 165 24 160 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.16 -0.14 (2nd move error 0.05 0.20) Finished aligning to item in 2 rounds Trimming A by 170 24 160 20, reference by 165 24 160 20 6.9 -2.2 unbinned pixels; 17.2 21.3 nm along two shift axes; 7.3 27.2 nm on specimen axes Total_Shift = 14.430043 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 0.99 microns changed by -2.09 to target drift = 0.15 nm/sec Measured defocus = -1.07 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2834.tif 27 frames are being saved to U:\krios\20190717\PilA_2835.tif 27 frames are being saved to U:\krios\20190717\PilA_2836.tif 27 frames are being saved to U:\krios\20190717\PilA_2837.tif 27 frames are being saved to U:\krios\20190717\PilA_2838.tif 27 frames are being saved to U:\krios\20190717\PilA_2839.tif Running script 17 at item 543 (-250.95, -595.69) Remaining time to fill dewars is 30120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 171 22 160 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.23 -0.26 (2nd move error -0.02 0.07) Finished aligning to item in 1 rounds Trimming A by 185 22 150 20, reference by 171 22 160 20 1.2 5.1 unbinned pixels; 17.1 11.9 nm along two shift axes; -20.0 3.6 nm on specimen axes Total_Shift = 10.409357 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.34 microns changed by -3.54 to target drift = 0.11 nm/sec Measured defocus = -1.21 microns changed by 0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2840.tif 27 frames are being saved to U:\krios\20190717\PilA_2841.tif 27 frames are being saved to U:\krios\20190717\PilA_2842.tif 27 frames are being saved to U:\krios\20190717\PilA_2843.tif 27 frames are being saved to U:\krios\20190717\PilA_2844.tif 27 frames are being saved to U:\krios\20190717\PilA_2845.tif Running script 17 at item 544 (-260.15, -593.66) Remaining time to fill dewars is 30000 Dewars are NOT busy filling Trimming A by 165 20 20 175, reference by 139 23 20 195 Trimming A by 165 20 20 175, reference by 125 24 110 20 Trimming A by 165 20 20 175, reference by 21 160 20 165 Trimming A by 165 20 20 175, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.28 Trimming A by 175 21 20 110, reference by 139 23 20 195 Disparity in stage position after aligning to target position: -0.12 0.00 (2nd move error 0.12 0.26) Finished aligning to item in 2 rounds Trimming A by 175 21 20 115, reference by 175 21 20 110 4.0 -2.1 unbinned pixels; 12.8 11.2 nm along two shift axes; 7.2 15.9 nm on specimen axes Total_Shift = 8.95616 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.54 microns changed by -3.84 to target drift = 0.13 nm/sec Measured defocus = -1.28 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2846.tif 27 frames are being saved to U:\krios\20190717\PilA_2847.tif 27 frames are being saved to U:\krios\20190717\PilA_2848.tif 27 frames are being saved to U:\krios\20190717\PilA_2849.tif 27 frames are being saved to U:\krios\20190717\PilA_2850.tif 27 frames are being saved to U:\krios\20190717\PilA_2851.tif Running script 17 at item 545 (-257.59, -594.70) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 21 80 30, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.30 -0.55 (2nd move error -0.09 -0.22) Finished aligning to item in 1 rounds Trimming A by 130 20 80 30, reference by 135 21 80 30 -27.7 17.1 unbinned pixels; 99.2 73.9 nm along two shift axes; -60.8 -111.4 nm on specimen axes Total_Shift = 65.142115 Trimming A by 130 21 80 30, reference by 135 21 80 30 -5.2 11.4 unbinned pixels; 48.6 1.7 nm along two shift axes; -43.1 -22.7 nm on specimen axes Total_Shift = 25.012579 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Ending Navigator acquisition after current item Measured defocus = 1.15 microns changed by -2.55 to target drift = 0.05 nm/sec Measured defocus = -1.52 microns changed by 0.12 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2852.tif 27 frames are being saved to U:\krios\20190717\PilA_2853.tif 27 frames are being saved to U:\krios\20190717\PilA_2854.tif 27 frames are being saved to U:\krios\20190717\PilA_2855.tif 27 frames are being saved to U:\krios\20190717\PilA_2856.tif 27 frames are being saved to U:\krios\20190717\PilA_2857.tif Running script 17 at item 546 (-255.09, -596.48) Remaining time to fill dewars is 29700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 21 90 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.24 -0.48 (2nd move error -0.02 -0.13) Finished aligning to item in 1 rounds Trimming A by 110 22 90 20, reference by 115 21 90 20 -14.5 5.8 unbinned pixels; 40.2 43.4 nm along two shift axes; -19.5 -57.5 nm on specimen axes Total_Shift = 31.163147 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.24 microns changed by -3.74 to target drift = 0.05 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2858.tif 27 frames are being saved to U:\krios\20190717\PilA_2859.tif 27 frames are being saved to U:\krios\20190717\PilA_2860.tif 27 frames are being saved to U:\krios\20190717\PilA_2861.tif 27 frames are being saved to U:\krios\20190717\PilA_2862.tif 27 frames are being saved to U:\krios\20190717\PilA_2863.tif Running script 17 at item 547 (-252.60, -598.23) Remaining time to fill dewars is 29580 Dewars are NOT busy filling Trimming A by 135 24 115 20, reference by 139 23 20 195 Trimming A by 135 24 115 20, reference by 125 24 110 20 Trimming A by 135 24 115 20, reference by 21 160 20 165 Trimming A by 135 24 115 20, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.18 -0.30 Trimming A by 105 21 90 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.16 -0.41 (2nd move error 0.02 -0.13) Finished aligning to item in 2 rounds Trimming A by 105 21 90 20, reference by 105 21 90 20 -16.0 -0.3 unbinned pixels; 19.7 58.2 nm along two shift axes; 4.7 -62.2 nm on specimen axes Total_Shift = 32.033497 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.71 microns changed by -2.31 to target drift = 0.11 nm/sec Measured defocus = -1.59 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2864.tif 27 frames are being saved to U:\krios\20190717\PilA_2865.tif 27 frames are being saved to U:\krios\20190717\PilA_2866.tif 27 frames are being saved to U:\krios\20190717\PilA_2867.tif 27 frames are being saved to U:\krios\20190717\PilA_2868.tif 27 frames are being saved to U:\krios\20190717\PilA_2869.tif Running script 17 at item 548 (-250.11, -599.99) Remaining time to fill dewars is 29400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 81 20 110 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.18 -0.25 (2nd move error -0.01 0.02) Finished aligning to item in 1 rounds Trimming A by 95 24 100 20, reference by 81 20 110 20 -1.6 10.5 unbinned pixels; 40.7 9.8 nm along two shift axes; -40.4 -8.6 nm on specimen axes Total_Shift = 21.204813 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.88 microns changed by -3.58 to target drift = 0.10 nm/sec Measured defocus = -1.72 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2870.tif 27 frames are being saved to U:\krios\20190717\PilA_2871.tif 27 frames are being saved to U:\krios\20190717\PilA_2872.tif 27 frames are being saved to U:\krios\20190717\PilA_2873.tif 27 frames are being saved to U:\krios\20190717\PilA_2874.tif 27 frames are being saved to U:\krios\20190717\PilA_2875.tif Running script 17 at item 549 (-247.72, -601.66) Remaining time to fill dewars is 29280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 80 41 120 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.10 -0.15 (2nd move error 0.07 0.13) Finished aligning to item in 1 rounds Trimming A by 85 31 125 20, reference by 80 41 120 20 3.7 2.4 unbinned pixels; 3.9 17.1 nm along two shift axes; -10.1 14.1 nm on specimen axes Total_Shift = 8.884949 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.27 microns changed by -2.07 to target drift = 0.14 nm/sec Measured defocus = -1.68 microns changed by -0.12 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2876.tif 27 frames are being saved to U:\krios\20190717\PilA_2877.tif 27 frames are being saved to U:\krios\20190717\PilA_2878.tif 27 frames are being saved to U:\krios\20190717\PilA_2879.tif 27 frames are being saved to U:\krios\20190717\PilA_2880.tif 27 frames are being saved to U:\krios\20190717\PilA_2881.tif Running script 17 at item 550 (-246.68, -605.96) Remaining time to fill dewars is 29100 Dewars are NOT busy filling Trimming A by 21 102 125 20, reference by 139 23 20 195 Trimming A by 21 102 125 20, reference by 125 24 110 20 Trimming A by 21 102 125 20, reference by 21 160 20 165 Trimming A by 21 102 125 20, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.13 -0.39 Trimming A by 21 105 195 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.20 -0.05 (2nd move error -0.08 0.32) Finished aligning to item in 2 rounds Trimming A by 21 104 190 20, reference by 21 105 195 20 2.1 20.7 unbinned pixels; 73.4 38.8 nm along two shift axes; -81.1 3.8 nm on specimen axes Total_Shift = 41.660392 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.67 microns changed by -3.57 to target drift = 0.15 nm/sec Measured defocus = -1.95 microns changed by 0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2882.tif 27 frames are being saved to U:\krios\20190717\PilA_2883.tif 27 frames are being saved to U:\krios\20190717\PilA_2884.tif 27 frames are being saved to U:\krios\20190717\PilA_2885.tif 27 frames are being saved to U:\krios\20190717\PilA_2886.tif 27 frames are being saved to U:\krios\20190717\PilA_2887.tif Running script 17 at item 551 (-249.19, -604.26) Remaining time to fill dewars is 28980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 36 120 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.21 -0.32 (2nd move error -0.08 0.06) Finished aligning to item in 1 rounds Trimming A by 21 31 120 20, reference by 24 36 120 20 1.4 15.6 unbinned pixels; 55.4 28.4 nm along two shift axes; -60.8 2.1 nm on specimen axes Total_Shift = 31.24744 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.26 microns changed by -2.26 to target drift = 0.12 nm/sec Measured defocus = -1.96 microns changed by -0.04 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2888.tif 27 frames are being saved to U:\krios\20190717\PilA_2889.tif 27 frames are being saved to U:\krios\20190717\PilA_2890.tif 27 frames are being saved to U:\krios\20190717\PilA_2891.tif 27 frames are being saved to U:\krios\20190717\PilA_2892.tif 27 frames are being saved to U:\krios\20190717\PilA_2893.tif Running script 17 at item 552 (-251.71, -602.46) Remaining time to fill dewars is 28860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 30 20 30 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.06 -0.44 (2nd move error 0.11 -0.17) Finished aligning to item in 1 rounds Trimming A by 30 20 30 20, reference by 30 20 30 20 -19.2 -0.0 unbinned pixels; 25.0 69.2 nm along two shift axes; 4.1 -74.7 nm on specimen axes Total_Shift = 38.422271 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.60 microns changed by -3.70 to target drift = 0.10 nm/sec Measured defocus = -2.17 microns changed by 0.07 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2894.tif 27 frames are being saved to U:\krios\20190717\PilA_2895.tif 27 frames are being saved to U:\krios\20190717\PilA_2896.tif 27 frames are being saved to U:\krios\20190717\PilA_2897.tif 27 frames are being saved to U:\krios\20190717\PilA_2898.tif 27 frames are being saved to U:\krios\20190717\PilA_2899.tif Running script 17 at item 553 (-254.23, -600.78) Remaining time to fill dewars is 28800 Dewars are NOT busy filling Trimming A by 135 24 120 20, reference by 139 23 20 195 Trimming A by 135 24 120 20, reference by 125 24 110 20 Trimming A by 135 24 120 20, reference by 21 160 20 165 Trimming A by 135 24 120 20, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.15 -0.40 Trimming A by 30 20 30 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.20 -0.53 (2nd move error -0.05 -0.15) Finished aligning to item in 2 rounds Trimming A by 30 20 25 20, reference by 30 20 30 20 -16.8 10.3 unbinned pixels; 59.9 45.0 nm along two shift axes; -36.6 -67.6 nm on specimen axes Total_Shift = 39.470969 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.16 microns changed by -2.36 to target drift = 0.04 nm/sec Measured defocus = -2.15 microns changed by -0.05 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2900.tif 27 frames are being saved to U:\krios\20190717\PilA_2901.tif 27 frames are being saved to U:\krios\20190717\PilA_2902.tif 27 frames are being saved to U:\krios\20190717\PilA_2903.tif 27 frames are being saved to U:\krios\20190717\PilA_2904.tif 27 frames are being saved to U:\krios\20190717\PilA_2905.tif Running script 17 at item 554 (-256.78, -599.07) Remaining time to fill dewars is 28560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 40 20 25 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.25 -0.62 (2nd move error -0.11 -0.23) Finished aligning to item in 1 rounds Trimming A by 40 20 20 20, reference by 40 20 25 20 -28.2 20.5 unbinned pixels; 112.3 70.5 nm along two shift axes; -74.0 -114.0 nm on specimen axes Total_Shift = 69.757812 Trimming A by 35 20 20 20, reference by 40 20 25 20 -4.1 13.3 unbinned pixels; 54.1 5.2 nm along two shift axes; -50.7 -18.7 nm on specimen axes Total_Shift = 27.752945 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.54 microns changed by -3.84 to target drift = 0.03 nm/sec Measured defocus = -2.34 microns changed by 0.04 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2906.tif 27 frames are being saved to U:\krios\20190717\PilA_2907.tif 27 frames are being saved to U:\krios\20190717\PilA_2908.tif 27 frames are being saved to U:\krios\20190717\PilA_2909.tif 27 frames are being saved to U:\krios\20190717\PilA_2910.tif 27 frames are being saved to U:\krios\20190717\PilA_2911.tif Running script 17 at item 555 (-259.33, -597.92) Remaining time to fill dewars is 28440 Dewars are NOT busy filling Trimming A by 139 21 20 195, reference by 139 23 20 195 Trimming A by 139 21 20 195, reference by 125 24 110 20 Trimming A by 139 21 20 195, reference by 21 160 20 165 Trimming A by 139 21 20 195, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.24 -0.30 Trimming A by 70 20 20 65, reference by 139 23 20 195 Disparity in stage position after aligning to target position: -0.04 -0.02 (2nd move error 0.20 0.27) Finished aligning to item in 2 rounds Trimming A by 70 20 20 65, reference by 70 20 20 65 2.4 5.2 unbinned pixels; 16.1 16.4 nm along two shift axes; -20.8 8.1 nm on specimen axes Total_Shift = 11.46803 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.16 microns changed by -2.56 to target drift = 0.12 nm/sec Measured defocus = -2.40 microns changed by 0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2912.tif 27 frames are being saved to U:\krios\20190717\PilA_2913.tif 27 frames are being saved to U:\krios\20190717\PilA_2914.tif 27 frames are being saved to U:\krios\20190717\PilA_2915.tif 27 frames are being saved to U:\krios\20190717\PilA_2916.tif 27 frames are being saved to U:\krios\20190717\PilA_2917.tif Running script 17 at item 556 (-261.79, -596.27) Remaining time to fill dewars is 28260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 20 20 135, reference by 139 23 20 195 Disparity in stage position after aligning to target position: -0.21 -0.19 (2nd move error 0.02 0.10) Finished aligning to item in 1 rounds Trimming A by 140 22 20 130, reference by 130 20 20 135 0.8 0.0 unbinned pixels; 0.9 2.9 nm along two shift axes; -0.4 3.1 nm on specimen axes Total_Shift = 1.58836 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.12 microns changed by -2.62 to target drift = 0.11 nm/sec Measured defocus = -2.53 microns changed by 0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2918.tif 27 frames are being saved to U:\krios\20190717\PilA_2919.tif 27 frames are being saved to U:\krios\20190717\PilA_2920.tif 27 frames are being saved to U:\krios\20190717\PilA_2921.tif 27 frames are being saved to U:\krios\20190717\PilA_2922.tif 27 frames are being saved to U:\krios\20190717\PilA_2923.tif Running script 17 at item 557 (-263.39, -598.83) Remaining time to fill dewars is 28140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 111 20 20 150, reference by 139 23 20 195 Disparity in stage position after aligning to target position: -0.06 -0.27 (2nd move error 0.17 0.01) Finished aligning to item in 1 rounds Trimming A by 111 20 20 145, reference by 111 20 20 150 4.2 -4.4 unbinned pixels; 21.7 8.7 nm along two shift axes; 16.2 17.4 nm on specimen axes Total_Shift = 12.226769 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.15 microns changed by -2.75 to target drift = 0.14 nm/sec Measured defocus = -2.61 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2924.tif 27 frames are being saved to U:\krios\20190717\PilA_2925.tif 27 frames are being saved to U:\krios\20190717\PilA_2926.tif 27 frames are being saved to U:\krios\20190717\PilA_2927.tif 27 frames are being saved to U:\krios\20190717\PilA_2928.tif 27 frames are being saved to U:\krios\20190717\PilA_2929.tif Running script 17 at item 558 (-260.97, -600.53) Remaining time to fill dewars is 27960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 50 23 20 75, reference by 139 23 20 195 Disparity in stage position after aligning to target position: -0.08 -0.17 (2nd move error 0.16 0.13) Finished aligning to item in 1 rounds Trimming A by 50 23 20 75, reference by 50 23 20 75 3.7 -20.9 unbinned pixels; 81.5 18.2 nm along two shift axes; 80.3 18.7 nm on specimen axes Total_Shift = 42.343014 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.63 microns changed by -2.73 to target drift = 0.15 nm/sec Measured defocus = -1.13 microns changed by 0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2930.tif 27 frames are being saved to U:\krios\20190717\PilA_2931.tif 27 frames are being saved to U:\krios\20190717\PilA_2932.tif 27 frames are being saved to U:\krios\20190717\PilA_2933.tif 27 frames are being saved to U:\krios\20190717\PilA_2934.tif 27 frames are being saved to U:\krios\20190717\PilA_2935.tif Running script 17 at item 559 (-258.51, -602.15) Remaining time to fill dewars is 27840 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 139 23 20 195 Disparity in stage position after aligning to target position: 0.01 0.00 (2nd move error 0.24 0.28) Finished aligning to item in 1 rounds 3.9 0.4 unbinned pixels; 3.5 14.8 nm along two shift axes; -2.5 15.2 nm on specimen axes Total_Shift = 7.917477 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.77 microns changed by -3.97 to target drift = 0.12 nm/sec Measured defocus = -1.20 microns changed by 0.00 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2936.tif 27 frames are being saved to U:\krios\20190717\PilA_2937.tif 27 frames are being saved to U:\krios\20190717\PilA_2938.tif 27 frames are being saved to U:\krios\20190717\PilA_2939.tif 27 frames are being saved to U:\krios\20190717\PilA_2940.tif 27 frames are being saved to U:\krios\20190717\PilA_2941.tif Running script 17 at item 560 (-255.91, -603.25) Remaining time to fill dewars is 27720 Dewars are NOT busy filling Trimming A by 135 24 115 20, reference by 139 23 20 195 Trimming A by 135 24 115 20, reference by 125 24 110 20 Trimming A by 135 24 115 20, reference by 21 160 20 165 Trimming A by 135 24 115 20, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.18 -0.32 Trimming A by 20 20 20 20, reference by 125 24 110 20 Disparity in stage position after aligning to target position: -0.13 -0.60 (2nd move error 0.04 -0.29) Finished aligning to item in 2 rounds -32.2 -3.9 unbinned pixels; 27.8 121.8 nm along two shift axes; 21.9 -124.5 nm on specimen axes Total_Shift = 64.898346 -3.4 -1.9 unbinned pixels; 2.5 15.0 nm along two shift axes; 8.0 -12.7 nm on specimen axes Total_Shift = 7.719414 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.29 microns changed by -2.59 to target drift = 0.15 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2942.tif 27 frames are being saved to U:\krios\20190717\PilA_2943.tif 27 frames are being saved to U:\krios\20190717\PilA_2944.tif 27 frames are being saved to U:\krios\20190717\PilA_2945.tif 27 frames are being saved to U:\krios\20190717\PilA_2946.tif 27 frames are being saved to U:\krios\20190717\PilA_2947.tif Running script 17 at item 561 (-253.31, -604.98) Remaining time to fill dewars is 27540 Dewars are NOT busy filling Trimming A by 20 120 110 20, reference by 139 23 20 195 Trimming A by 20 120 110 20, reference by 125 24 110 20 Trimming A by 20 120 110 20, reference by 21 160 20 165 Trimming A by 20 120 110 20, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.16 -0.38 Trimming A by 20 20 20 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.20 -0.52 (2nd move error -0.05 -0.16) Finished aligning to item in 2 rounds -22.7 14.6 unbinned pixels; 83.5 59.8 nm along two shift axes; -52.2 -91.5 nm on specimen axes Total_Shift = 54.084966 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.47 microns changed by -3.87 to target drift = 0.06 nm/sec Measured defocus = -1.47 microns changed by 0.07 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2948.tif 27 frames are being saved to U:\krios\20190717\PilA_2949.tif 27 frames are being saved to U:\krios\20190717\PilA_2950.tif 27 frames are being saved to U:\krios\20190717\PilA_2951.tif 27 frames are being saved to U:\krios\20190717\PilA_2952.tif 27 frames are being saved to U:\krios\20190717\PilA_2953.tif Running script 17 at item 562 (-250.86, -606.78) Remaining time to fill dewars is 27300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 48 100 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.16 -0.37 (2nd move error -0.00 -0.02) Finished aligning to item in 1 rounds Trimming A by 20 61 90 20, reference by 24 48 100 20 -4.5 2.0 unbinned pixels; 13.3 13.0 nm along two shift axes; -7.0 -17.8 nm on specimen axes Total_Shift = 9.802322 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.95 microns changed by -2.45 to target drift = 0.07 nm/sec Measured defocus = -1.53 microns changed by 0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2954.tif 27 frames are being saved to U:\krios\20190717\PilA_2955.tif 27 frames are being saved to U:\krios\20190717\PilA_2956.tif 27 frames are being saved to U:\krios\20190717\PilA_2957.tif 27 frames are being saved to U:\krios\20190717\PilA_2958.tif 27 frames are being saved to U:\krios\20190717\PilA_2959.tif Running script 17 at item 563 (-248.33, -608.55) Remaining time to fill dewars is 27240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 140 155 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.10 -0.20 (2nd move error 0.06 0.15) Finished aligning to item in 1 rounds Trimming A by 23 126 165 20, reference by 21 140 155 20 -1.2 -1.5 unbinned pixels; 4.1 6.5 nm along two shift axes; 6.2 -4.2 nm on specimen axes Total_Shift = 3.822842 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.06 microns changed by -3.66 to target drift = 0.12 nm/sec Measured defocus = -1.69 microns changed by 0.09 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2960.tif 27 frames are being saved to U:\krios\20190717\PilA_2961.tif 27 frames are being saved to U:\krios\20190717\PilA_2962.tif 27 frames are being saved to U:\krios\20190717\PilA_2963.tif 27 frames are being saved to U:\krios\20190717\PilA_2964.tif 27 frames are being saved to U:\krios\20190717\PilA_2965.tif Running script 17 at item 564 (-249.98, -611.09) Remaining time to fill dewars is 27120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 136 155 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.11 -0.36 (2nd move error 0.04 -0.01) Finished aligning to item in 1 rounds Trimming A by 23 150 145 20, reference by 21 136 155 20 -2.9 -3.4 unbinned pixels; 8.6 15.4 nm along two shift axes; 13.7 -10.5 nm on specimen axes Total_Shift = 8.861013 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.78 microns changed by -2.48 to target drift = 0.10 nm/sec Measured defocus = -1.76 microns changed by 0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2966.tif 27 frames are being saved to U:\krios\20190717\PilA_2967.tif 27 frames are being saved to U:\krios\20190717\PilA_2968.tif 27 frames are being saved to U:\krios\20190717\PilA_2969.tif 27 frames are being saved to U:\krios\20190717\PilA_2970.tif 27 frames are being saved to U:\krios\20190717\PilA_2971.tif Running script 17 at item 565 (-252.51, -609.35) Remaining time to fill dewars is 27000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 75 75 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.08 -0.50 (2nd move error 0.08 -0.15) Finished aligning to item in 1 rounds Trimming A by 23 80 75 20, reference by 24 75 75 20 -14.7 -3.9 unbinned pixels; 4.9 58.9 nm along two shift axes; 18.3 -56.4 nm on specimen axes Total_Shift = 30.452094 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 2.04 microns changed by -3.84 to target drift = 0.10 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2972.tif 27 frames are being saved to U:\krios\20190717\PilA_2973.tif 27 frames are being saved to U:\krios\20190717\PilA_2974.tif 27 frames are being saved to U:\krios\20190717\PilA_2975.tif 27 frames are being saved to U:\krios\20190717\PilA_2976.tif 27 frames are being saved to U:\krios\20190717\PilA_2977.tif Running script 17 at item 566 (-254.95, -607.60) Remaining time to fill dewars is 26820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.17 -0.59 (2nd move error -0.01 -0.24) Finished aligning to item in 1 rounds -29.3 5.8 unbinned pixels; 59.4 96.8 nm along two shift axes; -16.3 -115.2 nm on specimen axes Total_Shift = 59.702623 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.73 microns changed by -2.63 to target drift = 0.05 nm/sec Measured defocus = -1.94 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2978.tif Ending Navigator acquisition after current item 27 frames are being saved to U:\krios\20190717\PilA_2979.tif 27 frames are being saved to U:\krios\20190717\PilA_2980.tif 27 frames are being saved to U:\krios\20190717\PilA_2981.tif 27 frames are being saved to U:\krios\20190717\PilA_2982.tif 27 frames are being saved to U:\krios\20190717\PilA_2983.tif