Running script 17 at item 567 (-257.48, -605.82) Remaining time to fill dewars is 26580 Dewars are NOT busy filling Trimming A by 21 102 125 20, reference by 139 23 20 195 Trimming A by 21 102 125 20, reference by 125 24 110 20 Trimming A by 21 102 125 20, reference by 21 160 20 165 Trimming A by 21 102 125 20, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.07 -0.36 Trimming A by 20 20 20 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.27 -0.73 (2nd move error -0.21 -0.39) Finished aligning to item in 2 rounds -30.8 38.5 unbinned pixels; 182.0 52.8 nm along two shift axes; -143.5 -127.9 nm on specimen axes Total_Shift = 98.683752 -0.9 25.8 unbinned pixels; 96.1 35.4 nm along two shift axes; -100.2 -9.1 nm on specimen axes Total_Shift = 51.660833 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 2.03 microns changed by -4.03 to target drift = 0.04 nm/sec Measured defocus = -2.06 microns changed by 0.06 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2984.tif 27 frames are being saved to U:\krios\20190717\PilA_2985.tif 27 frames are being saved to U:\krios\20190717\PilA_2986.tif 27 frames are being saved to U:\krios\20190717\PilA_2987.tif 27 frames are being saved to U:\krios\20190717\PilA_2988.tif 27 frames are being saved to U:\krios\20190717\PilA_2989.tif Running script 17 at item 568 (-259.86, -604.87) Remaining time to fill dewars is 26400 Dewars are NOT busy filling Trimming A by 21 150 20 155, reference by 139 23 20 195 Trimming A by 21 150 20 155, reference by 125 24 110 20 Trimming A by 21 150 20 155, reference by 21 160 20 165 Trimming A by 21 150 20 155, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.35 Trimming A by 20 20 20 20, reference by 21 160 20 165 Disparity in stage position after aligning to target position: -0.20 -0.07 (2nd move error 0.14 0.26) Finished aligning to item in 2 rounds 2.9 0.6 unbinned pixels; 1.4 11.4 nm along two shift axes; -3.1 11.1 nm on specimen axes Total_Shift = 5.927085 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.69 microns changed by -2.79 to target drift = 0.14 nm/sec Measured defocus = -2.08 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2990.tif 27 frames are being saved to U:\krios\20190717\PilA_2991.tif 27 frames are being saved to U:\krios\20190717\PilA_2992.tif 27 frames are being saved to U:\krios\20190717\PilA_2993.tif 27 frames are being saved to U:\krios\20190717\PilA_2994.tif 27 frames are being saved to U:\krios\20190717\PilA_2995.tif Running script 17 at item 569 (-262.63, -603.09) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Trimming A by 155 20 20 185, reference by 139 23 20 195 Trimming A by 155 20 20 185, reference by 125 24 110 20 Trimming A by 155 20 20 185, reference by 21 160 20 165 Trimming A by 155 20 20 185, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.22 -0.37 Trimming A by 42 22 20 85, reference by 139 23 20 195 Disparity in stage position after aligning to target position: -0.06 -0.19 (2nd move error 0.15 0.15) Finished aligning to item in 2 rounds Trimming A by 50 23 20 75, reference by 42 22 20 85 -2.9 5.8 unbinned pixels; 25.0 1.5 nm along two shift axes; -21.9 -12.3 nm on specimen axes Total_Shift = 12.916703 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.72 microns changed by -2.92 to target drift = 0.09 nm/sec Measured defocus = -2.24 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_2996.tif 27 frames are being saved to U:\krios\20190717\PilA_2997.tif 27 frames are being saved to U:\krios\20190717\PilA_2998.tif 27 frames are being saved to U:\krios\20190717\PilA_2999.tif 27 frames are being saved to U:\krios\20190717\PilA_3000.tif 27 frames are being saved to U:\krios\20190717\PilA_3001.tif Running script 17 at item 570 (-265.07, -601.36) Remaining time to fill dewars is 26100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 89 24 20 165, reference by 139 23 20 195 Disparity in stage position after aligning to target position: -0.13 -0.35 (2nd move error 0.08 -0.00) Finished aligning to item in 1 rounds Trimming A by 101 22 20 155, reference by 89 24 20 165 -3.0 -2.2 unbinned pixels; 4.3 14.4 nm along two shift axes; 9.4 -11.4 nm on specimen axes Total_Shift = 7.569716 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.73 microns changed by -3.03 to target drift = 0.10 nm/sec Measured defocus = -2.39 microns changed by 0.10 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3002.tif 27 frames are being saved to U:\krios\20190717\PilA_3003.tif 27 frames are being saved to U:\krios\20190717\PilA_3004.tif 27 frames are being saved to U:\krios\20190717\PilA_3005.tif 27 frames are being saved to U:\krios\20190717\PilA_3006.tif 27 frames are being saved to U:\krios\20190717\PilA_3007.tif Running script 17 at item 571 (-266.84, -603.92) Remaining time to fill dewars is 25980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 79 22 20 185, reference by 139 23 20 195 Disparity in stage position after aligning to target position: -0.01 -0.41 (2nd move error 0.20 -0.06) Finished aligning to item in 1 rounds Trimming A by 71 21 20 185, reference by 79 22 20 185 -3.6 -2.0 unbinned pixels; 2.6 15.9 nm along two shift axes; 8.5 -13.5 nm on specimen axes Total_Shift = 8.193767 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.73 microns changed by -3.13 to target drift = 0.09 nm/sec Measured defocus = -2.47 microns changed by 0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3008.tif 27 frames are being saved to U:\krios\20190717\PilA_3009.tif 27 frames are being saved to U:\krios\20190717\PilA_3010.tif 27 frames are being saved to U:\krios\20190717\PilA_3011.tif 27 frames are being saved to U:\krios\20190717\PilA_3012.tif 27 frames are being saved to U:\krios\20190717\PilA_3013.tif Running script 17 at item 572 (-264.04, -605.63) Remaining time to fill dewars is 25740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 35 20 95, reference by 21 160 20 165 Disparity in stage position after aligning to target position: -0.29 -0.30 (2nd move error 0.05 0.02) Finished aligning to item in 1 rounds Trimming A by 23 23 20 105, reference by 35 35 20 95 0.1 -2.3 unbinned pixels; 8.5 2.9 nm along two shift axes; 8.8 1.0 nm on specimen axes Total_Shift = 4.540041 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.46 microns changed by -2.96 to target drift = 0.08 nm/sec Measured defocus = -2.52 microns changed by 0.02 to target drift = 0.07 nm/sec Ending Navigator acquisition after current item 27 frames are being saved to U:\krios\20190717\PilA_3014.tif 27 frames are being saved to U:\krios\20190717\PilA_3015.tif 27 frames are being saved to U:\krios\20190717\PilA_3016.tif 27 frames are being saved to U:\krios\20190717\PilA_3017.tif 27 frames are being saved to U:\krios\20190717\PilA_3018.tif 27 frames are being saved to U:\krios\20190717\PilA_3019.tif Running script 17 at item 573 (-261.46, -607.41) Remaining time to fill dewars is 25560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 55 20 70, reference by 21 160 20 165 Disparity in stage position after aligning to target position: -0.25 -0.15 (2nd move error 0.09 0.18) Finished aligning to item in 1 rounds Trimming A by 21 50 20 65, reference by 21 55 20 70 -4.8 -0.7 unbinned pixels; 3.6 18.4 nm along two shift axes; 3.8 -18.5 nm on specimen axes Total_Shift = 9.71002 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.28 microns changed by -2.88 to target drift = 0.10 nm/sec Measured defocus = -2.64 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3020.tif 27 frames are being saved to U:\krios\20190717\PilA_3021.tif 27 frames are being saved to U:\krios\20190717\PilA_3022.tif 27 frames are being saved to U:\krios\20190717\PilA_3023.tif 27 frames are being saved to U:\krios\20190717\PilA_3024.tif 27 frames are being saved to U:\krios\20190717\PilA_3025.tif Running script 17 at item 574 (-258.97, -609.05) Remaining time to fill dewars is 25440 Dewars are NOT busy filling Trimming A by 21 150 20 160, reference by 139 23 20 195 Trimming A by 21 150 20 160, reference by 125 24 110 20 Trimming A by 21 150 20 160, reference by 21 160 20 165 Trimming A by 21 150 20 160, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.22 -0.34 Trimming A by 20 60 20 65, reference by 21 160 20 165 Disparity in stage position after aligning to target position: -0.22 0.01 (2nd move error -0.01 0.33) Finished aligning to item in 2 rounds Trimming A by 21 55 40 55, reference by 20 60 20 65 5.2 5.7 unbinned pixels; 14.2 27.4 nm along two shift axes; -23.4 19.1 nm on specimen axes Total_Shift = 15.504332 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.78 microns changed by -2.88 to target drift = 0.17 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3026.tif 27 frames are being saved to U:\krios\20190717\PilA_3027.tif 27 frames are being saved to U:\krios\20190717\PilA_3028.tif 27 frames are being saved to U:\krios\20190717\PilA_3029.tif 27 frames are being saved to U:\krios\20190717\PilA_3030.tif 27 frames are being saved to U:\krios\20190717\PilA_3031.tif Running script 17 at item 575 (-256.61, -610.16) Remaining time to fill dewars is 25380 Dewars are NOT busy filling Trimming A by 20 120 110 20, reference by 139 23 20 195 Trimming A by 20 120 110 20, reference by 125 24 110 20 Trimming A by 20 120 110 20, reference by 21 160 20 165 Trimming A by 20 120 110 20, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.12 -0.32 Trimming A by 20 55 20 55, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.21 -0.64 (2nd move error -0.10 -0.35) Finished aligning to item in 2 rounds Trimming A by 21 60 40 55, reference by 20 55 20 55 -31.8 20.2 unbinned pixels; 115.6 84.0 nm along two shift axes; -71.8 -127.8 nm on specimen axes Total_Shift = 75.260945 Trimming A by 21 65 40 55, reference by 20 55 20 55 -3.2 12.3 unbinned pixels; 49.3 6.9 nm along two shift axes; -47.0 -15.1 nm on specimen axes Total_Shift = 25.350576 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 3.01 microns changed by -4.21 to target drift = 0.04 nm/sec Measured defocus = -1.32 microns changed by 0.12 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3032.tif 27 frames are being saved to U:\krios\20190717\PilA_3033.tif 27 frames are being saved to U:\krios\20190717\PilA_3034.tif 27 frames are being saved to U:\krios\20190717\PilA_3035.tif 27 frames are being saved to U:\krios\20190717\PilA_3036.tif 27 frames are being saved to U:\krios\20190717\PilA_3037.tif Running script 17 at item 576 (-254.11, -611.92) Remaining time to fill dewars is 25200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 95 70 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.14 -0.53 (2nd move error -0.03 -0.23) Finished aligning to item in 1 rounds Trimming A by 22 100 70 20, reference by 22 95 70 20 -23.7 20.4 unbinned pixels; 106.1 54.5 nm along two shift axes; -74.6 -96.5 nm on specimen axes Total_Shift = 62.605323 Trimming A by 21 91 80 35, reference by 22 95 70 20 -2.3 11.8 unbinned pixels; 46.2 9.5 nm along two shift axes; -45.2 -11.3 nm on specimen axes Total_Shift = 23.939575 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.44 microns changed by -2.74 to target drift = 0.03 nm/sec Measured defocus = -1.33 microns changed by 0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3038.tif 27 frames are being saved to U:\krios\20190717\PilA_3039.tif 27 frames are being saved to U:\krios\20190717\PilA_3040.tif 27 frames are being saved to U:\krios\20190717\PilA_3041.tif 27 frames are being saved to U:\krios\20190717\PilA_3042.tif 27 frames are being saved to U:\krios\20190717\PilA_3043.tif Running script 17 at item 577 (-251.67, -613.63) Remaining time to fill dewars is 25080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 157 140 20, reference by 23 106 125 20 Disparity in stage position after aligning to target position: -0.10 -0.40 (2nd move error 0.02 -0.09) Finished aligning to item in 1 rounds Trimming A by 20 170 130 20, reference by 20 157 140 20 -10.1 -2.9 unbinned pixels; 2.4 40.9 nm along two shift axes; 13.6 -38.8 nm on specimen axes Total_Shift = 21.102617 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.53 microns changed by -3.93 to target drift = 0.05 nm/sec Measured defocus = -1.42 microns changed by 0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3044.tif 27 frames are being saved to U:\krios\20190717\PilA_3045.tif 27 frames are being saved to U:\krios\20190717\PilA_3046.tif 27 frames are being saved to U:\krios\20190717\PilA_3047.tif 27 frames are being saved to U:\krios\20190717\PilA_3048.tif 27 frames are being saved to U:\krios\20190717\PilA_3049.tif Running script 17 at item 578 (-258.23, -613.28) Remaining time to fill dewars is 24900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 140 20 125, reference by 21 160 20 165 Disparity in stage position after aligning to target position: -0.16 -0.04 (2nd move error 0.04 0.28) Finished aligning to item in 1 rounds Trimming A by 20 135 20 115, reference by 20 140 20 125 4.0 0.1 unbinned pixels; 4.9 14.6 nm along two shift axes; -1.2 15.6 nm on specimen axes Total_Shift = 8.05386 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.36 microns changed by -2.86 to target drift = 0.09 nm/sec Measured defocus = -1.47 microns changed by -0.03 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3050.tif 27 frames are being saved to U:\krios\20190717\PilA_3051.tif 27 frames are being saved to U:\krios\20190717\PilA_3052.tif 27 frames are being saved to U:\krios\20190717\PilA_3053.tif 27 frames are being saved to U:\krios\20190717\PilA_3054.tif 27 frames are being saved to U:\krios\20190717\PilA_3055.tif Running script 17 at item 579 (-260.69, -611.68) Remaining time to fill dewars is 24780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 130 20 130, reference by 21 160 20 165 Disparity in stage position after aligning to target position: -0.17 -0.17 (2nd move error 0.03 0.16) Finished aligning to item in 1 rounds Trimming A by 23 125 20 125, reference by 22 130 20 130 -5.6 -1.6 unbinned pixels; 1.3 22.7 nm along two shift axes; 7.6 -21.5 nm on specimen axes Total_Shift = 11.724803 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.65 microns changed by -4.25 to target drift = 0.06 nm/sec Measured defocus = -1.65 microns changed by 0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3056.tif 27 frames are being saved to U:\krios\20190717\PilA_3057.tif 27 frames are being saved to U:\krios\20190717\PilA_3058.tif 27 frames are being saved to U:\krios\20190717\PilA_3059.tif 27 frames are being saved to U:\krios\20190717\PilA_3060.tif 27 frames are being saved to U:\krios\20190717\PilA_3061.tif Running script 17 at item 580 (-263.16, -609.95) Remaining time to fill dewars is 24600 Dewars are NOT busy filling Trimming A by 21 150 20 160, reference by 139 23 20 195 Trimming A by 21 150 20 160, reference by 125 24 110 20 Trimming A by 21 150 20 160, reference by 21 160 20 165 Trimming A by 21 150 20 160, reference by 23 106 125 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.35 Trimming A by 22 125 20 130, reference by 21 160 20 165 Disparity in stage position after aligning to target position: -0.15 -0.25 (2nd move error 0.05 0.06) Finished aligning to item in 2 rounds Trimming A by 24 111 20 140, reference by 22 125 20 130 -3.0 0.5 unbinned pixels; 6.0 10.1 nm along two shift axes; -1.5 -11.9 nm on specimen axes Total_Shift = 6.173543 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.40 microns changed by -3.10 to target drift = 0.12 nm/sec Measured defocus = -1.74 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3062.tif 27 frames are being saved to U:\krios\20190717\PilA_3063.tif 27 frames are being saved to U:\krios\20190717\PilA_3064.tif 27 frames are being saved to U:\krios\20190717\PilA_3065.tif 27 frames are being saved to U:\krios\20190717\PilA_3066.tif 27 frames are being saved to U:\krios\20190717\PilA_3067.tif Running script 17 at item 581 (-265.67, -608.21) Remaining time to fill dewars is 24360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 110 20 150, reference by 21 160 20 165 Disparity in stage position after aligning to target position: -0.26 -0.39 (2nd move error -0.06 -0.07) Finished aligning to item in 1 rounds Trimming A by 20 110 20 150, reference by 20 110 20 150 -8.6 12.6 unbinned pixels; 57.4 12.1 nm along two shift axes; -47.0 -36.1 nm on specimen axes Total_Shift = 30.434088 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 2.64 microns changed by -4.44 to target drift = 0.11 nm/sec Measured defocus = -1.82 microns changed by 0.02 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3068.tif 27 frames are being saved to U:\krios\20190717\PilA_3069.tif 27 frames are being saved to U:\krios\20190717\PilA_3070.tif 27 frames are being saved to U:\krios\20190717\PilA_3071.tif 27 frames are being saved to U:\krios\20190717\PilA_3072.tif 27 frames are being saved to U:\krios\20190717\PilA_3073.tif Running script 17 at item 582 (-611.03, -103.33) Remaining time to fill dewars is 24300 Dewars are NOT busy filling Trimming A by 229 20 175 20, reference by 207 24 20 225 Trimming A by 229 20 175 20, reference by 220 22 160 20 Trimming A by 229 20 175 20, reference by 23 145 20 165 Trimming A by 229 20 175 20, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.19 Trimming A by 120 20 145 20, reference by 220 22 160 20 Disparity in stage position after aligning to target position: -0.22 -0.17 (2nd move error 0.14 -0.00) Finished aligning to item in 2 rounds Trimming A by 120 20 155 20, reference by 120 20 145 20 1.1 4.5 unbinned pixels; 15.1 10.7 nm along two shift axes; -17.7 3.3 nm on specimen axes Total_Shift = 9.247336 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.47 microns changed by -2.37 to target drift = 0.18 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3074.tif 27 frames are being saved to U:\krios\20190717\PilA_3075.tif 27 frames are being saved to U:\krios\20190717\PilA_3076.tif 27 frames are being saved to U:\krios\20190717\PilA_3077.tif 27 frames are being saved to U:\krios\20190717\PilA_3078.tif 27 frames are being saved to U:\krios\20190717\PilA_3079.tif Running script 17 at item 583 (-608.36, -105.03) Remaining time to fill dewars is 24060 Dewars are NOT busy filling Trimming A by 23 116 145 20, reference by 207 24 20 225 Trimming A by 23 116 145 20, reference by 220 22 160 20 Trimming A by 23 116 145 20, reference by 23 145 20 165 Trimming A by 23 116 145 20, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.36 -0.26 Trimming A by 87 67 205 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.30 -0.07 (2nd move error 0.05 0.17) Finished aligning to item in 2 rounds Trimming A by 100 75 200 20, reference by 87 67 205 20 -2.9 -2.0 unbinned pixels; 3.5 13.4 nm along two shift axes; 8.3 -10.8 nm on specimen axes Total_Shift = 7.016302 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.62 microns changed by -3.62 to target drift = 0.17 nm/sec Measured defocus = -2.09 microns changed by 0.09 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3080.tif 27 frames are being saved to U:\krios\20190717\PilA_3081.tif 27 frames are being saved to U:\krios\20190717\PilA_3082.tif 27 frames are being saved to U:\krios\20190717\PilA_3083.tif 27 frames are being saved to U:\krios\20190717\PilA_3084.tif 27 frames are being saved to U:\krios\20190717\PilA_3085.tif Running script 17 at item 584 (-613.57, -101.60) Remaining time to fill dewars is 23940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 20 120 20, reference by 220 22 160 20 Disparity in stage position after aligning to target position: -0.28 -0.31 (2nd move error 0.09 -0.15) Finished aligning to item in 1 rounds Trimming A by 130 20 120 20, reference by 130 20 120 20 -17.2 0.1 unbinned pixels; 22.8 61.6 nm along two shift axes; 3.2 -66.8 nm on specimen axes Total_Shift = 34.32776 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.28 microns changed by -2.38 to target drift = 0.10 nm/sec Measured defocus = -2.11 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3086.tif 27 frames are being saved to U:\krios\20190717\PilA_3087.tif 27 frames are being saved to U:\krios\20190717\PilA_3088.tif 27 frames are being saved to U:\krios\20190717\PilA_3089.tif 27 frames are being saved to U:\krios\20190717\PilA_3090.tif 27 frames are being saved to U:\krios\20190717\PilA_3091.tif Running script 17 at item 585 (-616.08, -99.98) Remaining time to fill dewars is 23760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 22 110 20, reference by 220 22 160 20 Disparity in stage position after aligning to target position: -0.25 -0.37 (2nd move error 0.12 -0.21) Finished aligning to item in 1 rounds Trimming A by 145 22 110 20, reference by 145 22 110 20 -24.7 -0.7 unbinned pixels; 29.5 90.0 nm along two shift axes; 8.0 -95.9 nm on specimen axes Total_Shift = 49.396155 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.62 microns changed by -3.82 to target drift = 0.11 nm/sec Measured defocus = -2.21 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3092.tif 27 frames are being saved to U:\krios\20190717\PilA_3093.tif 27 frames are being saved to U:\krios\20190717\PilA_3094.tif 27 frames are being saved to U:\krios\20190717\PilA_3095.tif 27 frames are being saved to U:\krios\20190717\PilA_3096.tif 27 frames are being saved to U:\krios\20190717\PilA_3097.tif Running script 17 at item 586 (-618.60, -98.24) Remaining time to fill dewars is 23580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 165 24 100 20, reference by 220 22 160 20 Disparity in stage position after aligning to target position: -0.33 -0.41 (2nd move error 0.05 -0.24) Finished aligning to item in 1 rounds Trimming A by 160 24 100 20, reference by 165 24 100 20 -29.9 -0.4 unbinned pixels; 37.7 108.3 nm along two shift axes; 7.8 -116.4 nm on specimen axes Total_Shift = 59.874381 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.38 microns changed by -2.68 to target drift = 0.13 nm/sec Measured defocus = -2.40 microns changed by 0.10 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3098.tif 27 frames are being saved to U:\krios\20190717\PilA_3099.tif 27 frames are being saved to U:\krios\20190717\PilA_3100.tif 27 frames are being saved to U:\krios\20190717\PilA_3101.tif 27 frames are being saved to U:\krios\20190717\PilA_3102.tif 27 frames are being saved to U:\krios\20190717\PilA_3103.tif Running script 17 at item 587 (-622.82, -99.68) Remaining time to fill dewars is 23460 Dewars are NOT busy filling Trimming A by 215 21 20 215, reference by 207 24 20 225 Trimming A by 215 21 20 215, reference by 220 22 160 20 Trimming A by 215 21 20 215, reference by 23 145 20 165 Trimming A by 215 21 20 215, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.18 Trimming A by 141 22 20 140, reference by 207 24 20 225 Disparity in stage position after aligning to target position: -0.13 -0.05 (2nd move error 0.14 0.11) Finished aligning to item in 2 rounds Trimming A by 155 24 20 125, reference by 141 22 20 140 0.4 -3.3 unbinned pixels; 12.9 3.4 nm along two shift axes; 12.9 2.4 nm on specimen axes Total_Shift = 6.741501 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.56 microns changed by -3.96 to target drift = 0.15 nm/sec Measured defocus = -2.44 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3104.tif 27 frames are being saved to U:\krios\20190717\PilA_3105.tif 27 frames are being saved to U:\krios\20190717\PilA_3106.tif 27 frames are being saved to U:\krios\20190717\PilA_3107.tif 27 frames are being saved to U:\krios\20190717\PilA_3108.tif 27 frames are being saved to U:\krios\20190717\PilA_3109.tif Running script 17 at item 588 (-620.15, -100.86) Remaining time to fill dewars is 23340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 95 24 25 65, reference by 207 24 20 225 Disparity in stage position after aligning to target position: -0.09 0.02 (2nd move error 0.17 0.17) Finished aligning to item in 1 rounds Trimming A by 95 20 40 65, reference by 95 24 25 65 -0.2 -0.3 unbinned pixels; 0.8 1.3 nm along two shift axes; 1.2 -0.9 nm on specimen axes Total_Shift = 0.780622 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.06 microns changed by -2.56 to target drift = 0.15 nm/sec Measured defocus = -2.46 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3110.tif 27 frames are being saved to U:\krios\20190717\PilA_3111.tif 27 frames are being saved to U:\krios\20190717\PilA_3112.tif 27 frames are being saved to U:\krios\20190717\PilA_3113.tif 27 frames are being saved to U:\krios\20190717\PilA_3114.tif 27 frames are being saved to U:\krios\20190717\PilA_3115.tif Running script 17 at item 589 (-617.69, -102.52) Remaining time to fill dewars is 23040 Dewars are NOT busy filling Trimming A by 224 20 175 20, reference by 207 24 20 225 Trimming A by 224 20 175 20, reference by 220 22 160 20 Trimming A by 224 20 175 20, reference by 23 145 20 165 Trimming A by 224 20 175 20, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.16 Trimming A by 70 24 55 20, reference by 220 22 160 20 Disparity in stage position after aligning to target position: -0.20 -0.46 (2nd move error 0.08 -0.32) Finished aligning to item in 2 rounds Trimming A by 65 24 50 20, reference by 70 24 55 20 -30.8 -0.7 unbinned pixels; 37.7 111.9 nm along two shift axes; 9.2 -119.7 nm on specimen axes Total_Shift = 61.616957 Trimming A by 65 24 50 20, reference by 70 24 55 20 -1.3 -0.6 unbinned pixels; 0.4 5.6 nm along two shift axes; 2.5 -5.0 nm on specimen axes Total_Shift = 2.868649 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.04 microns changed by -2.56 to target drift = 0.10 nm/sec Measured defocus = -2.68 microns changed by 0.08 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3116.tif 27 frames are being saved to U:\krios\20190717\PilA_3117.tif 27 frames are being saved to U:\krios\20190717\PilA_3118.tif 27 frames are being saved to U:\krios\20190717\PilA_3119.tif 27 frames are being saved to U:\krios\20190717\PilA_3120.tif 27 frames are being saved to U:\krios\20190717\PilA_3121.tif Running script 17 at item 590 (-612.49, -105.81) Remaining time to fill dewars is 22980 Dewars are NOT busy filling Trimming A by 23 104 150 20, reference by 207 24 20 225 Trimming A by 23 104 150 20, reference by 220 22 160 20 Trimming A by 23 104 150 20, reference by 23 145 20 165 Trimming A by 23 104 150 20, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.27 Trimming A by 41 41 95 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.29 -0.29 (2nd move error -0.02 -0.04) Finished aligning to item in 2 rounds Trimming A by 40 40 95 20, reference by 41 41 95 20 -6.6 5.3 unbinned pixels; 28.2 15.6 nm along two shift axes; -19.4 -26.6 nm on specimen axes Total_Shift = 16.922537 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.26 microns changed by -2.36 to target drift = 0.06 nm/sec Measured defocus = -1.07 microns changed by -0.03 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3122.tif 27 frames are being saved to U:\krios\20190717\PilA_3123.tif 27 frames are being saved to U:\krios\20190717\PilA_3124.tif 27 frames are being saved to U:\krios\20190717\PilA_3125.tif 27 frames are being saved to U:\krios\20190717\PilA_3126.tif 27 frames are being saved to U:\krios\20190717\PilA_3127.tif Running script 17 at item 591 (-609.97, -107.58) Remaining time to fill dewars is 22860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 77 190 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.36 -0.12 (2nd move error -0.09 0.12) Finished aligning to item in 1 rounds Trimming A by 21 96 175 20, reference by 20 77 190 20 0.4 17.5 unbinned pixels; 63.9 27.9 nm along two shift axes; -68.3 -2.0 nm on specimen axes Total_Shift = 35.075808 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.43 microns changed by -3.63 to target drift = 0.16 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3128.tif 27 frames are being saved to U:\krios\20190717\PilA_3129.tif 27 frames are being saved to U:\krios\20190717\PilA_3130.tif 27 frames are being saved to U:\krios\20190717\PilA_3131.tif 27 frames are being saved to U:\krios\20190717\PilA_3132.tif 27 frames are being saved to U:\krios\20190717\PilA_3133.tif Running script 17 at item 592 (-611.58, -110.23) Remaining time to fill dewars is 22680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 103 165 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.18 -0.22 (2nd move error 0.09 0.02) Finished aligning to item in 1 rounds Trimming A by 20 120 155 20, reference by 21 103 165 20 0.9 -0.2 unbinned pixels; 1.8 2.8 nm along two shift axes; 0.5 3.4 nm on specimen axes Total_Shift = 1.765281 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.33 microns changed by -3.63 to target drift = 0.12 nm/sec Measured defocus = -1.28 microns changed by -0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3134.tif 27 frames are being saved to U:\krios\20190717\PilA_3135.tif 27 frames are being saved to U:\krios\20190717\PilA_3136.tif 27 frames are being saved to U:\krios\20190717\PilA_3137.tif 27 frames are being saved to U:\krios\20190717\PilA_3138.tif 27 frames are being saved to U:\krios\20190717\PilA_3139.tif Running script 17 at item 594 (-614.17, -108.44) Remaining time to fill dewars is 22560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 42 85 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.21 -0.36 (2nd move error 0.06 -0.12) Finished aligning to item in 1 rounds Trimming A by 21 46 85 20, reference by 20 42 85 20 -14.2 0.5 unbinned pixels; 20.3 50.5 nm along two shift axes; 1.1 -55.4 nm on specimen axes Total_Shift = 28.468492 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.06 microns changed by -2.46 to target drift = 0.10 nm/sec Measured defocus = -1.43 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3140.tif 27 frames are being saved to U:\krios\20190717\PilA_3141.tif 27 frames are being saved to U:\krios\20190717\PilA_3142.tif 27 frames are being saved to U:\krios\20190717\PilA_3143.tif 27 frames are being saved to U:\krios\20190717\PilA_3144.tif 27 frames are being saved to U:\krios\20190717\PilA_3145.tif Running script 17 at item 595 (-616.67, -106.74) Remaining time to fill dewars is 22260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.31 -0.47 (2nd move error -0.03 -0.23) Finished aligning to item in 1 rounds -29.6 9.4 unbinned pixels; 73.2 92.5 nm along two shift axes; -30.3 -117.3 nm on specimen axes Total_Shift = 62.170138 -4.7 5.3 unbinned pixels; 25.8 8.9 nm along two shift axes; -19.8 -19.4 nm on specimen axes Total_Shift = 14.219427 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.36 microns changed by -3.86 to target drift = 0.00 nm/sec Measured defocus = -1.55 microns changed by 0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3146.tif 27 frames are being saved to U:\krios\20190717\PilA_3147.tif 27 frames are being saved to U:\krios\20190717\PilA_3148.tif 27 frames are being saved to U:\krios\20190717\PilA_3149.tif 27 frames are being saved to U:\krios\20190717\PilA_3150.tif 27 frames are being saved to U:\krios\20190717\PilA_3151.tif Running script 17 at item 596 (-619.46, -105.02) Remaining time to fill dewars is 22140 Dewars are NOT busy filling Trimming A by 224 20 175 20, reference by 207 24 20 225 Trimming A by 224 20 175 20, reference by 220 22 160 20 Trimming A by 224 20 175 20, reference by 23 145 20 165 Trimming A by 224 20 175 20, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.22 -0.18 Trimming A by 20 20 20 20, reference by 220 22 160 20 Disparity in stage position after aligning to target position: -0.13 -0.59 (2nd move error 0.08 -0.42) Finished aligning to item in 2 rounds -21.0 -2.8 unbinned pixels; 17.1 79.7 nm along two shift axes; 15.3 -81.0 nm on specimen axes Total_Shift = 42.291444 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.02 microns changed by -2.62 to target drift = 0.14 nm/sec Measured defocus = -1.60 microns changed by -0.00 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3152.tif 27 frames are being saved to U:\krios\20190717\PilA_3153.tif 27 frames are being saved to U:\krios\20190717\PilA_3154.tif 27 frames are being saved to U:\krios\20190717\PilA_3155.tif 27 frames are being saved to U:\krios\20190717\PilA_3156.tif 27 frames are being saved to U:\krios\20190717\PilA_3157.tif Running script 17 at item 597 (-621.92, -103.90) Remaining time to fill dewars is 21960 Dewars are NOT busy filling Trimming A by 207 24 20 225, reference by 207 24 20 225 Trimming A by 207 24 20 225, reference by 220 22 160 20 Trimming A by 207 24 20 225, reference by 23 145 20 165 Trimming A by 207 24 20 225, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.26 -0.19 Trimming A by 47 22 20 85, reference by 207 24 20 225 Disparity in stage position after aligning to target position: -0.03 -0.01 (2nd move error 0.23 0.16) Finished aligning to item in 2 rounds Trimming A by 47 22 20 80, reference by 47 22 20 85 1.3 5.9 unbinned pixels; 19.9 13.7 nm along two shift axes; -23.2 4.0 nm on specimen axes Total_Shift = 12.101865 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 2.32 microns changed by -4.02 to target drift = 0.17 nm/sec Measured defocus = -1.74 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3158.tif 27 frames are being saved to U:\krios\20190717\PilA_3159.tif 27 frames are being saved to U:\krios\20190717\PilA_3160.tif 27 frames are being saved to U:\krios\20190717\PilA_3161.tif 27 frames are being saved to U:\krios\20190717\PilA_3162.tif 27 frames are being saved to U:\krios\20190717\PilA_3163.tif Running script 17 at item 598 (-624.36, -102.18) Remaining time to fill dewars is 21840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 116 24 20 150, reference by 207 24 20 225 Disparity in stage position after aligning to target position: -0.11 -0.13 (2nd move error 0.14 0.04) Finished aligning to item in 1 rounds Trimming A by 112 22 20 150, reference by 116 24 20 150 -1.2 -3.4 unbinned pixels; 10.9 9.2 nm along two shift axes; 13.3 -3.8 nm on specimen axes Total_Shift = 7.124775 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.89 microns changed by -2.69 to target drift = 0.11 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3164.tif 27 frames are being saved to U:\krios\20190717\PilA_3165.tif 27 frames are being saved to U:\krios\20190717\PilA_3166.tif 27 frames are being saved to U:\krios\20190717\PilA_3167.tif 27 frames are being saved to U:\krios\20190717\PilA_3168.tif 27 frames are being saved to U:\krios\20190717\PilA_3169.tif Running script 17 at item 599 (-626.05, -104.80) Remaining time to fill dewars is 21660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 105 23 20 160, reference by 207 24 20 225 Disparity in stage position after aligning to target position: -0.06 -0.24 (2nd move error 0.19 -0.07) Finished aligning to item in 1 rounds Trimming A by 80 21 20 170, reference by 105 23 20 160 -5.7 -2.4 unbinned pixels; 1.6 24.1 nm along two shift axes; 10.7 -21.5 nm on specimen axes Total_Shift = 12.349572 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 2.22 microns changed by -4.12 to target drift = 0.08 nm/sec Measured defocus = -1.93 microns changed by 0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3170.tif 27 frames are being saved to U:\krios\20190717\PilA_3171.tif 27 frames are being saved to U:\krios\20190717\PilA_3172.tif 27 frames are being saved to U:\krios\20190717\PilA_3173.tif 27 frames are being saved to U:\krios\20190717\PilA_3174.tif 27 frames are being saved to U:\krios\20190717\PilA_3175.tif Running script 17 at item 600 (-623.26, -106.40) Remaining time to fill dewars is 21480 Dewars are NOT busy filling Trimming A by 24 140 20 160, reference by 207 24 20 225 Trimming A by 24 140 20 160, reference by 220 22 160 20 Trimming A by 24 140 20 160, reference by 23 145 20 165 Trimming A by 24 140 20 160, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.16 -0.25 Trimming A by 35 24 20 80, reference by 23 145 20 165 Disparity in stage position after aligning to target position: -0.14 -0.06 (2nd move error 0.02 0.17) Finished aligning to item in 2 rounds Trimming A by 32 22 20 85, reference by 35 24 20 80 -3.8 0.9 unbinned pixels; 8.4 12.2 nm along two shift axes; -2.8 -14.9 nm on specimen axes Total_Shift = 7.77605 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.66 microns changed by -2.66 to target drift = 0.10 nm/sec Measured defocus = -2.00 microns changed by 0.00 to target drift = 0.08 nm/sec Ending Navigator acquisition after current item 27 frames are being saved to U:\krios\20190717\PilA_3176.tif 27 frames are being saved to U:\krios\20190717\PilA_3177.tif 27 frames are being saved to U:\krios\20190717\PilA_3178.tif 27 frames are being saved to U:\krios\20190717\PilA_3179.tif 27 frames are being saved to U:\krios\20190717\PilA_3180.tif 27 frames are being saved to U:\krios\20190717\PilA_3181.tif Running script 17 at item 601 (-620.81, -108.16) Remaining time to fill dewars is 21060 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 20 20 20 25, reference by 23 145 20 165 Disparity in stage position after aligning to target position: -0.14 -0.05 (2nd move error 0.01 0.17) Finished aligning to item in 1 rounds Trimming A by 20 20 20 20, reference by 20 20 20 25 1.1 0.9 unbinned pixels; 2.0 5.2 nm along two shift axes; -3.8 3.9 nm on specimen axes Total_Shift = 2.813261 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.71 microns changed by -3.81 to target drift = 0.12 nm/sec Measured defocus = -2.10 microns changed by -0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3182.tif 27 frames are being saved to U:\krios\20190717\PilA_3183.tif 27 frames are being saved to U:\krios\20190717\PilA_3184.tif 27 frames are being saved to U:\krios\20190717\PilA_3185.tif 27 frames are being saved to U:\krios\20190717\PilA_3186.tif 27 frames are being saved to U:\krios\20190717\PilA_3187.tif Running script 17 at item 602 (-618.30, -109.38) Remaining time to fill dewars is 20820 Dewars are NOT busy filling Trimming A by 23 104 150 20, reference by 207 24 20 225 Trimming A by 23 104 150 20, reference by 220 22 160 20 Trimming A by 23 104 150 20, reference by 23 145 20 165 Trimming A by 23 104 150 20, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.24 -0.23 Trimming A by 20 20 20 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.19 -0.53 (2nd move error 0.05 -0.32) Finished aligning to item in 2 rounds -23.5 -1.3 unbinned pixels; 26.0 86.7 nm along two shift axes; 10.0 -91.3 nm on specimen axes Total_Shift = 47.149498 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.32 microns changed by -2.52 to target drift = 0.14 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3188.tif 27 frames are being saved to U:\krios\20190717\PilA_3189.tif 27 frames are being saved to U:\krios\20190717\PilA_3190.tif 27 frames are being saved to U:\krios\20190717\PilA_3191.tif 27 frames are being saved to U:\krios\20190717\PilA_3192.tif 27 frames are being saved to U:\krios\20190717\PilA_3193.tif Running script 17 at item 603 (-615.74, -111.08) Remaining time to fill dewars is 20700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 61 75 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.16 -0.38 (2nd move error 0.08 -0.17) Finished aligning to item in 1 rounds Trimming A by 22 65 75 20, reference by 24 61 75 20 -20.5 0.7 unbinned pixels; 29.2 72.9 nm along two shift axes; 1.7 -80.0 nm on specimen axes Total_Shift = 41.089242 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.48 microns changed by -3.78 to target drift = 0.09 nm/sec Measured defocus = -2.39 microns changed by 0.09 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3194.tif 27 frames are being saved to U:\krios\20190717\PilA_3195.tif 27 frames are being saved to U:\krios\20190717\PilA_3196.tif 27 frames are being saved to U:\krios\20190717\PilA_3197.tif 27 frames are being saved to U:\krios\20190717\PilA_3198.tif 27 frames are being saved to U:\krios\20190717\PilA_3199.tif Running script 17 at item 604 (-613.25, -112.74) Remaining time to fill dewars is 20520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 145 130 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.20 -0.25 (2nd move error 0.03 -0.04) Finished aligning to item in 1 rounds Trimming A by 22 145 130 20, reference by 22 145 130 20 -6.1 -0.8 unbinned pixels; 5.1 23.3 nm along two shift axes; 4.4 -23.7 nm on specimen axes Total_Shift = 12.398415 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.15 microns changed by -2.25 to target drift = 0.10 nm/sec Measured defocus = -2.44 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3200.tif 27 frames are being saved to U:\krios\20190717\PilA_3201.tif 27 frames are being saved to U:\krios\20190717\PilA_3202.tif 27 frames are being saved to U:\krios\20190717\PilA_3203.tif 27 frames are being saved to U:\krios\20190717\PilA_3204.tif 27 frames are being saved to U:\krios\20190717\PilA_3205.tif Running script 17 at item 605 (-614.85, -115.35) Remaining time to fill dewars is 20280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 170 115 20, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.05 -0.34 (2nd move error 0.19 -0.13) Finished aligning to item in 1 rounds Trimming A by 21 170 120 20, reference by 24 170 115 20 -12.6 -2.5 unbinned pixels; 7.1 49.2 nm along two shift axes; 12.6 -48.5 nm on specimen axes Total_Shift = 25.730056 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.14 microns changed by -3.64 to target drift = 0.06 nm/sec Measured defocus = -2.51 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3206.tif 27 frames are being saved to U:\krios\20190717\PilA_3207.tif 27 frames are being saved to U:\krios\20190717\PilA_3208.tif 27 frames are being saved to U:\krios\20190717\PilA_3209.tif 27 frames are being saved to U:\krios\20190717\PilA_3210.tif 27 frames are being saved to U:\krios\20190717\PilA_3211.tif Running script 17 at item 606 (-617.39, -113.66) Remaining time to fill dewars is 20040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 105 60 65, reference by 21 116 135 20 Disparity in stage position after aligning to target position: -0.14 -0.47 (2nd move error 0.09 -0.26) Finished aligning to item in 1 rounds Trimming A by 21 105 55 70, reference by 21 105 60 65 -23.1 1.2 unbinned pixels; 34.7 81.4 nm along two shift axes; 0.2 -90.2 nm on specimen axes Total_Shift = 46.323065 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.16 microns changed by -2.44 to target drift = 0.14 nm/sec Measured defocus = -2.63 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3212.tif 27 frames are being saved to U:\krios\20190717\PilA_3213.tif 27 frames are being saved to U:\krios\20190717\PilA_3214.tif 27 frames are being saved to U:\krios\20190717\PilA_3215.tif 27 frames are being saved to U:\krios\20190717\PilA_3216.tif 27 frames are being saved to U:\krios\20190717\PilA_3217.tif Running script 17 at item 607 (-619.88, -112.22) Remaining time to fill dewars is 19920 Dewars are NOT busy filling Trimming A by 24 140 20 160, reference by 207 24 20 225 Trimming A by 24 140 20 160, reference by 220 22 160 20 Trimming A by 24 140 20 160, reference by 23 145 20 165 Trimming A by 24 140 20 160, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.21 -0.20 Trimming A by 24 90 20 85, reference by 23 145 20 165 Disparity in stage position after aligning to target position: -0.05 0.03 (2nd move error 0.15 0.21) Finished aligning to item in 2 rounds Trimming A by 24 85 20 80, reference by 24 90 20 85 1.5 -2.3 unbinned pixels; 10.2 1.9 nm along two shift axes; 8.4 6.2 nm on specimen axes Total_Shift = 5.367032 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.69 microns changed by -2.59 to target drift = 0.14 nm/sec Measured defocus = -0.86 microns changed by -0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3218.tif 27 frames are being saved to U:\krios\20190717\PilA_3219.tif 27 frames are being saved to U:\krios\20190717\PilA_3220.tif 27 frames are being saved to U:\krios\20190717\PilA_3221.tif 27 frames are being saved to U:\krios\20190717\PilA_3222.tif 27 frames are being saved to U:\krios\20190717\PilA_3223.tif Running script 17 at item 608 (-622.39, -110.72) Remaining time to fill dewars is 19740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 85 20 95, reference by 23 145 20 165 Disparity in stage position after aligning to target position: -0.10 -0.11 (2nd move error 0.10 0.07) Finished aligning to item in 1 rounds Trimming A by 24 85 20 90, reference by 24 85 20 95 0.4 1.0 unbinned pixels; 3.4 2.8 nm along two shift axes; -4.1 1.1 nm on specimen axes Total_Shift = 2.196331 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.89 microns changed by -3.89 to target drift = 0.15 nm/sec Measured defocus = -0.91 microns changed by -0.09 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3224.tif 27 frames are being saved to U:\krios\20190717\PilA_3225.tif 27 frames are being saved to U:\krios\20190717\PilA_3226.tif 27 frames are being saved to U:\krios\20190717\PilA_3227.tif 27 frames are being saved to U:\krios\20190717\PilA_3228.tif 27 frames are being saved to U:\krios\20190717\PilA_3229.tif Running script 17 at item 609 (-624.86, -108.98) Remaining time to fill dewars is 19440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 70 20 105, reference by 23 145 20 165 Disparity in stage position after aligning to target position: -0.22 -0.22 (2nd move error -0.02 -0.04) Finished aligning to item in 1 rounds Trimming A by 21 70 20 105, reference by 21 70 20 105 -5.8 5.1 unbinned pixels; 26.4 13.2 nm along two shift axes; -18.7 -23.7 nm on specimen axes Total_Shift = 15.507017 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.69 microns changed by -2.79 to target drift = 0.08 nm/sec Measured defocus = -1.06 microns changed by -0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3230.tif 27 frames are being saved to U:\krios\20190717\PilA_3231.tif 27 frames are being saved to U:\krios\20190717\PilA_3232.tif 27 frames are being saved to U:\krios\20190717\PilA_3233.tif 27 frames are being saved to U:\krios\20190717\PilA_3234.tif 27 frames are being saved to U:\krios\20190717\PilA_3235.tif Running script 17 at item 611 (-627.46, -107.23) Remaining time to fill dewars is 19140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 69 24 20 185, reference by 23 145 20 165 Disparity in stage position after aligning to target position: -0.25 -0.26 (2nd move error -0.05 -0.08) Finished aligning to item in 1 rounds Trimming A by 60 24 20 190, reference by 69 24 20 185 -9.1 10.7 unbinned pixels; 51.1 16.5 nm along two shift axes; -39.6 -37.5 nm on specimen axes Total_Shift = 27.995217 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 3.04 microns changed by -4.24 to target drift = 0.11 nm/sec Measured defocus = -1.24 microns changed by 0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3236.tif 27 frames are being saved to U:\krios\20190717\PilA_3237.tif 27 frames are being saved to U:\krios\20190717\PilA_3238.tif 27 frames are being saved to U:\krios\20190717\PilA_3239.tif 27 frames are being saved to U:\krios\20190717\PilA_3240.tif 27 frames are being saved to U:\krios\20190717\PilA_3241.tif Running script 17 at item 612 (-626.52, -111.63) Remaining time to fill dewars is 19020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 135 20 180, reference by 23 145 20 165 Disparity in stage position after aligning to target position: -0.15 -0.26 (2nd move error 0.05 -0.08) Finished aligning to item in 1 rounds Trimming A by 20 140 20 175, reference by 21 135 20 180 -8.4 -0.6 unbinned pixels; 9.0 31.1 nm along two shift axes; 3.9 -32.6 nm on specimen axes Total_Shift = 16.878147 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.56 microns changed by -2.86 to target drift = 0.08 nm/sec Measured defocus = -1.29 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3242.tif 27 frames are being saved to U:\krios\20190717\PilA_3243.tif 27 frames are being saved to U:\krios\20190717\PilA_3244.tif 27 frames are being saved to U:\krios\20190717\PilA_3245.tif 27 frames are being saved to U:\krios\20190717\PilA_3246.tif 27 frames are being saved to U:\krios\20190717\PilA_3247.tif Running script 17 at item 613 (-624.03, -113.33) Remaining time to fill dewars is 18780 Dewars are NOT busy filling Trimming A by 24 140 20 160, reference by 207 24 20 225 Trimming A by 24 140 20 160, reference by 220 22 160 20 Trimming A by 24 140 20 160, reference by 23 145 20 165 Trimming A by 24 140 20 160, reference by 21 116 135 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.24 Trimming A by 21 155 20 160, reference by 23 145 20 165 Disparity in stage position after aligning to target position: -0.07 -0.18 (2nd move error 0.03 0.04) Finished aligning to item in 2 rounds Trimming A by 21 160 75 150, reference by 21 155 20 160 -3.2 -0.1 unbinned pixels; 3.9 11.6 nm along two shift axes; 1.0 -12.4 nm on specimen axes Total_Shift = 6.404991 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.62 microns changed by -4.02 to target drift = 0.07 nm/sec Measured defocus = -1.42 microns changed by 0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3248.tif 27 frames are being saved to U:\krios\20190717\PilA_3249.tif 27 frames are being saved to U:\krios\20190717\PilA_3250.tif 27 frames are being saved to U:\krios\20190717\PilA_3251.tif 27 frames are being saved to U:\krios\20190717\PilA_3252.tif 27 frames are being saved to U:\krios\20190717\PilA_3253.tif Running script 17 at item 614 (-621.53, -115.00) Remaining time to fill dewars is 18540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 170 20 145, reference by 23 145 20 165 Disparity in stage position after aligning to target position: -0.07 -0.02 (2nd move error 0.04 0.20) Finished aligning to item in 1 rounds Trimming A by 24 165 20 140, reference by 22 170 20 145 -2.4 -0.4 unbinned pixels; 1.9 9.3 nm along two shift axes; 1.9 -9.4 nm on specimen axes Total_Shift = 4.931546 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.12 microns changed by -2.62 to target drift = 0.10 nm/sec Measured defocus = -1.53 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3254.tif 27 frames are being saved to U:\krios\20190717\PilA_3255.tif 27 frames are being saved to U:\krios\20190717\PilA_3256.tif 27 frames are being saved to U:\krios\20190717\PilA_3257.tif 27 frames are being saved to U:\krios\20190717\PilA_3258.tif 27 frames are being saved to U:\krios\20190717\PilA_3259.tif Running script 17 at item 615 (-755.19, -243.48) Remaining time to fill dewars is 18420 Dewars are NOT busy filling Trimming A by 21 175 170 20, reference by 205 23 20 210 Trimming A by 21 175 170 20, reference by 210 23 170 20 Trimming A by 21 175 170 20, reference by 23 185 20 185 Trimming A by 21 175 170 20, reference by 24 180 160 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.26 Trimming A by 56 70 155 20, reference by 24 180 160 20 Disparity in stage position after aligning to target position: -0.45 -0.21 (2nd move error -0.09 0.03) Finished aligning to item in 2 rounds Trimming A by 56 70 155 20, reference by 56 70 155 20 -9.9 27.4 unbinned pixels; 113.5 5.7 nm along two shift axes; -104.4 -44.2 nm on specimen axes Total_Shift = 58.200453 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.95 microns changed by -3.55 to target drift = 0.10 nm/sec Measured defocus = -1.65 microns changed by 0.05 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3260.tif 27 frames are being saved to U:\krios\20190717\PilA_3261.tif 27 frames are being saved to U:\krios\20190717\PilA_3262.tif 27 frames are being saved to U:\krios\20190717\PilA_3263.tif 27 frames are being saved to U:\krios\20190717\PilA_3264.tif 27 frames are being saved to U:\krios\20190717\PilA_3265.tif Running script 17 at item 616 (-757.83, -241.81) Remaining time to fill dewars is 18120 Dewars are NOT busy filling Trimming A by 207 23 195 20, reference by 205 23 20 210 Trimming A by 207 23 195 20, reference by 210 23 170 20 Trimming A by 207 23 195 20, reference by 23 185 20 185 Trimming A by 207 23 195 20, reference by 24 180 160 20 Disparity in stage position after aligning to center of map frame: -0.39 -0.27 Trimming A by 85 20 100 20, reference by 210 23 170 20 Disparity in stage position after aligning to target position: -0.30 -0.32 (2nd move error 0.07 -0.07) Finished aligning to item in 2 rounds Trimming A by 85 20 100 20, reference by 85 20 100 20 -7.7 -0.2 unbinned pixels; 9.2 27.9 nm along two shift axes; 2.5 -29.7 nm on specimen axes Total_Shift = 15.31844 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.55 microns changed by -2.25 to target drift = 0.08 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3266.tif 27 frames are being saved to U:\krios\20190717\PilA_3267.tif 27 frames are being saved to U:\krios\20190717\PilA_3268.tif 27 frames are being saved to U:\krios\20190717\PilA_3269.tif 27 frames are being saved to U:\krios\20190717\PilA_3270.tif 27 frames are being saved to U:\krios\20190717\PilA_3271.tif Running script 17 at item 617 (-760.31, -240.08) Remaining time to fill dewars is 17940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 95 23 85 20, reference by 210 23 170 20 Disparity in stage position after aligning to target position: -0.37 -0.41 (2nd move error 0.01 -0.17) Finished aligning to item in 1 rounds Trimming A by 95 23 80 20, reference by 95 23 85 20 -20.2 0.7 unbinned pixels; 28.9 71.9 nm along two shift axes; 1.7 -78.9 nm on specimen axes Total_Shift = 40.503347 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.83 microns changed by -3.63 to target drift = 0.05 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3272.tif 27 frames are being saved to U:\krios\20190717\PilA_3273.tif 27 frames are being saved to U:\krios\20190717\PilA_3274.tif 27 frames are being saved to U:\krios\20190717\PilA_3275.tif 27 frames are being saved to U:\krios\20190717\PilA_3276.tif 27 frames are being saved to U:\krios\20190717\PilA_3277.tif Running script 17 at item 618 (-762.80, -238.44) Remaining time to fill dewars is 17700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 110 21 70 20, reference by 210 23 170 20 Disparity in stage position after aligning to target position: -0.42 -0.48 (2nd move error -0.05 -0.24) Finished aligning to item in 1 rounds Trimming A by 105 22 70 20, reference by 110 21 70 20 -26.1 15.6 unbinned pixels; 91.3 70.4 nm along two shift axes; -55.1 -104.7 nm on specimen axes Total_Shift = 60.73114 Trimming A by 105 22 65 20, reference by 110 21 70 20 -3.6 10.1 unbinned pixels; 41.9 2.3 nm along two shift axes; -38.6 -16.1 nm on specimen axes Total_Shift = 21.484912 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.56 microns changed by -2.46 to target drift = 0.05 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3278.tif 27 frames are being saved to U:\krios\20190717\PilA_3279.tif 27 frames are being saved to U:\krios\20190717\PilA_3280.tif 27 frames are being saved to U:\krios\20190717\PilA_3281.tif 27 frames are being saved to U:\krios\20190717\PilA_3282.tif 27 frames are being saved to U:\krios\20190717\PilA_3283.tif Running script 17 at item 619 (-765.35, -237.25) Remaining time to fill dewars is 17580 Dewars are NOT busy filling Trimming A by 205 22 20 205, reference by 205 23 20 210 Trimming A by 205 22 20 205, reference by 210 23 170 20 Trimming A by 205 22 20 205, reference by 23 185 20 185 Trimming A by 205 22 20 205, reference by 24 180 160 20 Disparity in stage position after aligning to center of map frame: -0.36 -0.17 Trimming A by 150 24 20 105, reference by 205 23 20 210 Disparity in stage position after aligning to target position: -0.31 0.03 (2nd move error 0.05 0.17) Finished aligning to item in 2 rounds Trimming A by 155 21 75 100, reference by 150 24 20 105 -1.5 1.7 unbinned pixels; 8.4 2.9 nm along two shift axes; -6.4 -6.3 nm on specimen axes Total_Shift = 4.629583 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.76 microns changed by -3.76 to target drift = 0.18 nm/sec Measured defocus = -2.07 microns changed by 0.07 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3284.tif 27 frames are being saved to U:\krios\20190717\PilA_3285.tif 27 frames are being saved to U:\krios\20190717\PilA_3286.tif 27 frames are being saved to U:\krios\20190717\PilA_3287.tif 27 frames are being saved to U:\krios\20190717\PilA_3288.tif 27 frames are being saved to U:\krios\20190717\PilA_3289.tif Running script 17 at item 620 (-766.97, -239.84) Remaining time to fill dewars is 17280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 102 22 20 130, reference by 205 23 20 210 Disparity in stage position after aligning to target position: -0.31 -0.09 (2nd move error 0.04 0.06) Finished aligning to item in 1 rounds Trimming A by 102 23 25 130, reference by 102 22 20 130 -1.0 -1.5 unbinned pixels; 4.1 5.6 nm along two shift axes; 5.8 -3.4 nm on specimen axes Total_Shift = 3.47633 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.47 microns changed by -2.57 to target drift = 0.13 nm/sec Measured defocus = -2.11 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3290.tif 27 frames are being saved to U:\krios\20190717\PilA_3291.tif 27 frames are being saved to U:\krios\20190717\PilA_3292.tif 27 frames are being saved to U:\krios\20190717\PilA_3293.tif 27 frames are being saved to U:\krios\20190717\PilA_3294.tif 27 frames are being saved to U:\krios\20190717\PilA_3295.tif Running script 17 at item 621 (-764.60, -241.42) Remaining time to fill dewars is 17100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 21 20 50, reference by 205 23 20 210 Disparity in stage position after aligning to target position: -0.27 0.04 (2nd move error 0.08 0.19) Finished aligning to item in 1 rounds Trimming A by 55 21 20 55, reference by 55 21 20 50 2.0 -0.5 unbinned pixels; 4.6 6.3 nm along two shift axes; 1.7 7.8 nm on specimen axes Total_Shift = 4.093765 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.58 microns changed by -3.78 to target drift = 0.16 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3296.tif 27 frames are being saved to U:\krios\20190717\PilA_3297.tif 27 frames are being saved to U:\krios\20190717\PilA_3298.tif 27 frames are being saved to U:\krios\20190717\PilA_3299.tif 27 frames are being saved to U:\krios\20190717\PilA_3300.tif 27 frames are being saved to U:\krios\20190717\PilA_3301.tif Running script 17 at item 622 (-761.98, -242.67) Remaining time to fill dewars is 16920 Dewars are NOT busy filling Trimming A by 220 23 180 20, reference by 205 23 20 210 Trimming A by 220 23 180 20, reference by 210 23 170 20 Trimming A by 220 23 180 20, reference by 23 185 20 185 Trimming A by 220 23 180 20, reference by 24 180 160 20 Disparity in stage position after aligning to center of map frame: -0.39 -0.21 Trimming A by 25 20 20 20, reference by 210 23 170 20 Disparity in stage position after aligning to target position: -0.36 -0.43 (2nd move error 0.01 -0.23) Finished aligning to item in 2 rounds Trimming A by 20 20 20 20, reference by 25 20 20 20 -27.3 0.4 unbinned pixels; 37.1 97.5 nm along two shift axes; 4.1 -106.1 nm on specimen axes Total_Shift = 54.522095 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.20 microns changed by -2.50 to target drift = 0.10 nm/sec Measured defocus = -2.41 microns changed by 0.11 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3302.tif 27 frames are being saved to U:\krios\20190717\PilA_3303.tif 27 frames are being saved to U:\krios\20190717\PilA_3304.tif 27 frames are being saved to U:\krios\20190717\PilA_3305.tif 27 frames are being saved to U:\krios\20190717\PilA_3306.tif 27 frames are being saved to U:\krios\20190717\PilA_3307.tif Running script 17 at item 623 (-759.29, -244.31) Remaining time to fill dewars is 16680 Dewars are NOT busy filling Trimming A by 24 180 170 20, reference by 205 23 20 210 Trimming A by 24 180 170 20, reference by 210 23 170 20 Trimming A by 24 180 170 20, reference by 23 185 20 185 Trimming A by 24 180 170 20, reference by 24 180 160 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.23 Trimming A by 21 21 70 20, reference by 24 180 160 20 Disparity in stage position after aligning to target position: -0.47 -0.38 (2nd move error -0.11 -0.18) Finished aligning to item in 2 rounds Trimming A by 21 21 70 20, reference by 21 21 70 20 -20.8 22.8 unbinned pixels; 111.2 40.5 nm along two shift axes; -84.5 -85.8 nm on specimen axes Total_Shift = 61.806497 Trimming A by 21 21 70 20, reference by 21 21 70 20 -4.8 14.2 unbinned pixels; 58.4 4.0 nm along two shift axes; -54.2 -21.7 nm on specimen axes Total_Shift = 29.958093 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.29 microns changed by -3.69 to target drift = 0.04 nm/sec Measured defocus = -2.49 microns changed by 0.09 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3308.tif 27 frames are being saved to U:\krios\20190717\PilA_3309.tif 27 frames are being saved to U:\krios\20190717\PilA_3310.tif 27 frames are being saved to U:\krios\20190717\PilA_3311.tif 27 frames are being saved to U:\krios\20190717\PilA_3312.tif 27 frames are being saved to U:\krios\20190717\PilA_3313.tif Running script 17 at item 624 (-756.75, -246.07) Remaining time to fill dewars is 16440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 66 155 20, reference by 24 180 160 20 Disparity in stage position after aligning to target position: -0.39 -0.28 (2nd move error -0.04 -0.08) Finished aligning to item in 1 rounds Trimming A by 22 64 155 20, reference by 23 66 155 20 -8.8 8.9 unbinned pixels; 44.3 18.2 nm along two shift axes; -32.9 -36.1 nm on specimen axes Total_Shift = 25.058308 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.32 microns changed by -2.18 to target drift = 0.06 nm/sec Measured defocus = -2.49 microns changed by -0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3314.tif 27 frames are being saved to U:\krios\20190717\PilA_3315.tif 27 frames are being saved to U:\krios\20190717\PilA_3316.tif 27 frames are being saved to U:\krios\20190717\PilA_3317.tif 27 frames are being saved to U:\krios\20190717\PilA_3318.tif 27 frames are being saved to U:\krios\20190717\PilA_3319.tif Running script 17 at item 625 (-758.42, -248.67) Remaining time to fill dewars is 16260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 125 110 20, reference by 24 180 160 20 Disparity in stage position after aligning to target position: -0.36 -0.38 (2nd move error 0.01 -0.18) Finished aligning to item in 1 rounds Trimming A by 22 125 110 20, reference by 20 125 110 20 -22.0 1.0 unbinned pixels; 32.5 77.8 nm along two shift axes; 0.7 -86.0 nm on specimen axes Total_Shift = 44.125533 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 1.04 microns changed by -3.64 to target drift = 0.08 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3320.tif 27 frames are being saved to U:\krios\20190717\PilA_3321.tif 27 frames are being saved to U:\krios\20190717\PilA_3322.tif 27 frames are being saved to U:\krios\20190717\PilA_3323.tif 27 frames are being saved to U:\krios\20190717\PilA_3324.tif 27 frames are being saved to U:\krios\20190717\PilA_3325.tif Running script 17 at item 626 (-760.95, -246.95) Remaining time to fill dewars is 16080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 55 45 20, reference by 24 180 160 20 Disparity in stage position after aligning to target position: -0.43 -0.45 (2nd move error -0.07 -0.24) Finished aligning to item in 1 rounds Trimming A by 20 60 45 20, reference by 20 55 45 20 -27.8 16.3 unbinned pixels; 96.1 75.6 nm along two shift axes; -57.4 -111.6 nm on specimen axes Total_Shift = 64.412601 Trimming A by 20 60 45 20, reference by 20 55 45 20 -2.3 10.9 unbinned pixels; 43.3 8.1 nm along two shift axes; -42.1 -11.3 nm on specimen axes Total_Shift = 22.377401 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.53 microns changed by -2.43 to target drift = 0.06 nm/sec Measured defocus = -0.91 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3326.tif 27 frames are being saved to U:\krios\20190717\PilA_3327.tif 27 frames are being saved to U:\krios\20190717\PilA_3328.tif 27 frames are being saved to U:\krios\20190717\PilA_3329.tif 27 frames are being saved to U:\krios\20190717\PilA_3330.tif 27 frames are being saved to U:\krios\20190717\PilA_3331.tif Running script 17 at item 627 (-763.77, -245.48) Remaining time to fill dewars is 15900 Dewars are NOT busy filling Trimming A by 22 180 20 180, reference by 205 23 20 210 Trimming A by 22 180 20 180, reference by 210 23 170 20 Trimming A by 22 180 20 180, reference by 23 185 20 185 Trimming A by 22 180 20 180, reference by 24 180 160 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.21 Trimming A by 21 25 20 25, reference by 23 185 20 185 Disparity in stage position after aligning to target position: -0.30 0.01 (2nd move error 0.06 0.20) Finished aligning to item in 2 rounds Trimming A by 20 20 20 20, reference by 21 25 20 25 1.5 1.3 unbinned pixels; 2.7 7.3 nm along two shift axes; -5.2 5.6 nm on specimen axes Total_Shift = 3.929731 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.91 microns changed by -3.91 to target drift = 0.15 nm/sec Measured defocus = -1.07 microns changed by 0.07 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3332.tif 27 frames are being saved to U:\krios\20190717\PilA_3333.tif 27 frames are being saved to U:\krios\20190717\PilA_3334.tif 27 frames are being saved to U:\krios\20190717\PilA_3335.tif 27 frames are being saved to U:\krios\20190717\PilA_3336.tif 27 frames are being saved to U:\krios\20190717\PilA_3337.tif Running script 17 at item 628 (-766.00, -243.94) Remaining time to fill dewars is 15600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 30 20 20 60, reference by 23 185 20 185 Disparity in stage position after aligning to target position: -0.35 -0.12 (2nd move error 0.01 0.07) Finished aligning to item in 1 rounds Trimming A by 30 20 20 60, reference by 30 20 20 60 -0.1 0.3 unbinned pixels; 1.4 0.2 nm along two shift axes; -1.3 -0.4 nm on specimen axes Total_Shift = 0.717932 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.87 microns changed by -3.97 to target drift = 0.18 nm/sec Measured defocus = -1.12 microns changed by 0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3338.tif 27 frames are being saved to U:\krios\20190717\PilA_3339.tif 27 frames are being saved to U:\krios\20190717\PilA_3340.tif 27 frames are being saved to U:\krios\20190717\PilA_3341.tif 27 frames are being saved to U:\krios\20190717\PilA_3342.tif 27 frames are being saved to U:\krios\20190717\PilA_3343.tif Running script 17 at item 629 (-768.60, -242.34) Remaining time to fill dewars is 15480 Dewars are NOT busy filling Trimming A by 197 24 20 215, reference by 205 23 20 210 Trimming A by 197 24 20 215, reference by 210 23 170 20 Trimming A by 197 24 20 215, reference by 23 185 20 185 Trimming A by 197 24 20 215, reference by 24 180 160 20 Disparity in stage position after aligning to center of map frame: -0.36 -0.21 Trimming A by 64 21 20 150, reference by 205 23 20 210 Disparity in stage position after aligning to target position: -0.29 -0.16 (2nd move error 0.06 0.02) Finished aligning to item in 2 rounds Trimming A by 56 23 20 155, reference by 64 21 20 150 1.4 -1.2 unbinned pixels; 6.1 3.2 nm along two shift axes; 4.2 5.6 nm on specimen axes Total_Shift = 3.599506 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.53 microns changed by -2.73 to target drift = 0.15 nm/sec Measured defocus = -1.17 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3344.tif 27 frames are being saved to U:\krios\20190717\PilA_3345.tif 27 frames are being saved to U:\krios\20190717\PilA_3346.tif 27 frames are being saved to U:\krios\20190717\PilA_3347.tif 27 frames are being saved to U:\krios\20190717\PilA_3348.tif 27 frames are being saved to U:\krios\20190717\PilA_3349.tif Running script 17 at item 630 (-770.15, -244.92) Remaining time to fill dewars is 15300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 48 34 20 165, reference by 23 185 20 185 Disparity in stage position after aligning to target position: -0.39 -0.29 (2nd move error -0.03 -0.10) Finished aligning to item in 1 rounds Trimming A by 40 30 20 170, reference by 48 34 20 165 -12.5 8.6 unbinned pixels; 48.1 32.0 nm along two shift axes; -31.0 -50.4 nm on specimen axes Total_Shift = 30.368978 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.89 microns changed by -4.19 to target drift = 0.09 nm/sec Measured defocus = -1.35 microns changed by 0.05 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3350.tif 27 frames are being saved to U:\krios\20190717\PilA_3351.tif 27 frames are being saved to U:\krios\20190717\PilA_3352.tif 27 frames are being saved to U:\krios\20190717\PilA_3353.tif 27 frames are being saved to U:\krios\20190717\PilA_3354.tif 27 frames are being saved to U:\krios\20190717\PilA_3355.tif Running script 17 at item 631 (-767.56, -246.65) Remaining time to fill dewars is 15060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 20 105, reference by 23 185 20 185 Disparity in stage position after aligning to target position: -0.41 -0.17 (2nd move error -0.05 0.02) Finished aligning to item in 1 rounds Trimming A by 20 80 20 105, reference by 20 80 20 105 2.0 10.2 unbinned pixels; 35.1 22.4 nm along two shift axes; -40.2 5.4 nm on specimen axes Total_Shift = 20.833115 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.32 microns changed by -2.72 to target drift = 0.13 nm/sec Measured defocus = -1.35 microns changed by -0.05 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3356.tif 27 frames are being saved to U:\krios\20190717\PilA_3357.tif 27 frames are being saved to U:\krios\20190717\PilA_3358.tif 27 frames are being saved to U:\krios\20190717\PilA_3359.tif 27 frames are being saved to U:\krios\20190717\PilA_3360.tif 27 frames are being saved to U:\krios\20190717\PilA_3361.tif Running script 17 at item 632 (-765.04, -248.34) Remaining time to fill dewars is 14880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 95 20 90, reference by 23 185 20 185 Disparity in stage position after aligning to target position: -0.33 -0.00 (2nd move error 0.03 0.19) Finished aligning to item in 1 rounds Trimming A by 24 90 20 90, reference by 24 95 20 90 1.9 -0.1 unbinned pixels; 3.0 6.4 nm along two shift axes; 0.2 7.2 nm on specimen axes Total_Shift = 3.713915 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.46 microns changed by -3.96 to target drift = 0.17 nm/sec Measured defocus = -1.54 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3362.tif 27 frames are being saved to U:\krios\20190717\PilA_3363.tif 27 frames are being saved to U:\krios\20190717\PilA_3364.tif 27 frames are being saved to U:\krios\20190717\PilA_3365.tif 27 frames are being saved to U:\krios\20190717\PilA_3366.tif 27 frames are being saved to U:\krios\20190717\PilA_3367.tif Running script 17 at item 633 (-762.53, -249.53) Remaining time to fill dewars is 14700 Dewars are NOT busy filling Trimming A by 24 180 170 20, reference by 205 23 20 210 Trimming A by 24 180 170 20, reference by 210 23 170 20 Trimming A by 24 180 170 20, reference by 23 185 20 185 Trimming A by 24 180 170 20, reference by 24 180 160 20 Disparity in stage position after aligning to center of map frame: -0.34 -0.21 Trimming A by 24 100 20 80, reference by 24 180 160 20 Disparity in stage position after aligning to target position: -0.44 -0.49 (2nd move error -0.10 -0.30) Finished aligning to item in 2 rounds Trimming A by 21 110 60 75, reference by 24 100 20 80 -30.6 20.1 unbinned pixels; 113.8 79.8 nm along two shift axes; -71.7 -123.1 nm on specimen axes Total_Shift = 73.133385 Trimming A by 23 110 20 80, reference by 24 100 20 80 -1.0 13.1 unbinned pixels; 49.4 16.0 nm along two shift axes; -50.6 -6.7 nm on specimen axes Total_Shift = 26.207235 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.02 microns changed by -2.62 to target drift = 0.05 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3368.tif 27 frames are being saved to U:\krios\20190717\PilA_3369.tif 27 frames are being saved to U:\krios\20190717\PilA_3370.tif 27 frames are being saved to U:\krios\20190717\PilA_3371.tif 27 frames are being saved to U:\krios\20190717\PilA_3372.tif 27 frames are being saved to U:\krios\20190717\PilA_3373.tif Running script 17 at item 634 (-759.97, -251.29) Remaining time to fill dewars is 14520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 150 105 30, reference by 24 180 160 20 Disparity in stage position after aligning to target position: -0.32 -0.37 (2nd move error 0.01 -0.19) Finished aligning to item in 1 rounds Trimming A by 20 160 100 20, reference by 20 150 105 30 -22.0 -0.3 unbinned pixels; 27.6 79.5 nm along two shift axes; 5.8 -85.4 nm on specimen axes Total_Shift = 43.945837 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 2.14 microns changed by -3.84 to target drift = 0.06 nm/sec Measured defocus = -1.77 microns changed by 0.07 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3374.tif 27 frames are being saved to U:\krios\20190717\PilA_3375.tif 27 frames are being saved to U:\krios\20190717\PilA_3376.tif 27 frames are being saved to U:\krios\20190717\PilA_3377.tif 27 frames are being saved to U:\krios\20190717\PilA_3378.tif 27 frames are being saved to U:\krios\20190717\PilA_3379.tif Running script 17 at item 635 (-724.36, -187.10) Remaining time to fill dewars is 14280 Dewars are NOT busy filling Trimming A by 24 160 160 20, reference by 215 20 20 200 Trimming A by 24 160 160 20, reference by 220 24 165 20 Trimming A by 24 160 160 20, reference by 23 155 20 165 Trimming A by 24 160 160 20, reference by 24 145 160 20 Disparity in stage position after aligning to center of map frame: -0.47 -0.29 Trimming A by 62 62 160 20, reference by 24 145 160 20 Disparity in stage position after aligning to target position: -0.51 -0.22 (2nd move error -0.05 0.05) Finished aligning to item in 2 rounds Trimming A by 62 62 160 20, reference by 62 62 160 20 -0.0 11.6 unbinned pixels; 42.7 17.3 nm along two shift axes; -45.1 -2.6 nm on specimen axes Total_Shift = 23.192865 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.43 microns changed by -2.23 to target drift = 0.18 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3380.tif 27 frames are being saved to U:\krios\20190717\PilA_3381.tif 27 frames are being saved to U:\krios\20190717\PilA_3382.tif 27 frames are being saved to U:\krios\20190717\PilA_3383.tif 27 frames are being saved to U:\krios\20190717\PilA_3384.tif 27 frames are being saved to U:\krios\20190717\PilA_3385.tif Running script 17 at item 636 (-727.11, -185.36) Remaining time to fill dewars is 14100 Dewars are NOT busy filling Trimming A by 229 23 180 20, reference by 215 20 20 200 Trimming A by 229 23 180 20, reference by 220 24 165 20 Trimming A by 229 23 180 20, reference by 23 155 20 165 Trimming A by 229 23 180 20, reference by 24 145 160 20 Disparity in stage position after aligning to center of map frame: -0.46 -0.29 Trimming A by 90 24 105 20, reference by 220 24 165 20 Disparity in stage position after aligning to target position: -0.35 -0.29 (2nd move error 0.11 -0.02) Finished aligning to item in 2 rounds Trimming A by 90 20 110 20, reference by 90 24 105 20 -2.3 3.3 unbinned pixels; 15.2 3.2 nm along two shift axes; -12.5 -9.6 nm on specimen axes Total_Shift = 8.071616 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.83 microns changed by -3.73 to target drift = 0.07 nm/sec Measured defocus = -1.99 microns changed by 0.09 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3386.tif 27 frames are being saved to U:\krios\20190717\PilA_3387.tif 27 frames are being saved to U:\krios\20190717\PilA_3388.tif 27 frames are being saved to U:\krios\20190717\PilA_3389.tif 27 frames are being saved to U:\krios\20190717\PilA_3390.tif 27 frames are being saved to U:\krios\20190717\PilA_3391.tif Running script 17 at item 637 (-729.58, -183.67) Remaining time to fill dewars is 13920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 105 22 85 20, reference by 220 24 165 20 Disparity in stage position after aligning to target position: -0.39 -0.36 (2nd move error 0.06 -0.09) Finished aligning to item in 1 rounds Trimming A by 105 22 85 20, reference by 105 22 85 20 -12.3 0.0 unbinned pixels; 16.2 44.2 nm along two shift axes; 2.5 -47.8 nm on specimen axes Total_Shift = 24.593928 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.40 microns changed by -2.40 to target drift = 0.08 nm/sec Measured defocus = -2.00 microns changed by 0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3392.tif 27 frames are being saved to U:\krios\20190717\PilA_3393.tif 27 frames are being saved to U:\krios\20190717\PilA_3394.tif 27 frames are being saved to U:\krios\20190717\PilA_3395.tif 27 frames are being saved to U:\krios\20190717\PilA_3396.tif 27 frames are being saved to U:\krios\20190717\PilA_3397.tif Running script 17 at item 638 (-732.07, -181.95) Remaining time to fill dewars is 13680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 120 20 75 20, reference by 220 24 165 20 Disparity in stage position after aligning to target position: -0.47 -0.49 (2nd move error -0.02 -0.22) Finished aligning to item in 1 rounds Trimming A by 120 20 75 20, reference by 120 20 75 20 -29.1 7.3 unbinned pixels; 64.8 93.7 nm along two shift axes; -22.3 -114.7 nm on specimen axes Total_Shift = 59.997989 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.73 microns changed by -3.83 to target drift = 0.02 nm/sec Measured defocus = -2.14 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3398.tif 27 frames are being saved to U:\krios\20190717\PilA_3399.tif 27 frames are being saved to U:\krios\20190717\PilA_3400.tif 27 frames are being saved to U:\krios\20190717\PilA_3401.tif 27 frames are being saved to U:\krios\20190717\PilA_3402.tif 27 frames are being saved to U:\krios\20190717\PilA_3403.tif Running script 17 at item 639 (-734.72, -180.78) Remaining time to fill dewars is 13500 Dewars are NOT busy filling Trimming A by 215 23 20 195, reference by 215 20 20 200 Trimming A by 215 23 20 195, reference by 220 24 165 20 Trimming A by 215 23 20 195, reference by 23 155 20 165 Trimming A by 215 23 20 195, reference by 24 145 160 20 Disparity in stage position after aligning to center of map frame: -0.33 -0.18 Trimming A by 150 20 20 100, reference by 215 20 20 200 Disparity in stage position after aligning to target position: -0.19 0.01 (2nd move error 0.13 0.16) Finished aligning to item in 2 rounds Trimming A by 150 20 25 100, reference by 150 20 20 100 2.2 0.8 unbinned pixels; 0.2 9.2 nm along two shift axes; -3.4 8.6 nm on specimen axes Total_Shift = 4.744447 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.30 microns changed by -2.50 to target drift = 0.16 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3404.tif 27 frames are being saved to U:\krios\20190717\PilA_3405.tif 27 frames are being saved to U:\krios\20190717\PilA_3406.tif 27 frames are being saved to U:\krios\20190717\PilA_3407.tif 27 frames are being saved to U:\krios\20190717\PilA_3408.tif 27 frames are being saved to U:\krios\20190717\PilA_3409.tif Running script 17 at item 640 (-736.35, -183.36) Remaining time to fill dewars is 13260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 23 20 105, reference by 215 20 20 200 Disparity in stage position after aligning to target position: -0.26 -0.09 (2nd move error 0.06 0.06) Finished aligning to item in 1 rounds Trimming A by 115 23 20 105, reference by 115 23 20 105 -0.4 -1.4 unbinned pixels; 4.7 3.7 nm along two shift axes; 5.7 -1.4 nm on specimen axes Total_Shift = 2.995336 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.58 microns changed by -3.88 to target drift = 0.14 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3410.tif 27 frames are being saved to U:\krios\20190717\PilA_3411.tif 27 frames are being saved to U:\krios\20190717\PilA_3412.tif 27 frames are being saved to U:\krios\20190717\PilA_3413.tif 27 frames are being saved to U:\krios\20190717\PilA_3414.tif 27 frames are being saved to U:\krios\20190717\PilA_3415.tif Running script 17 at item 641 (-733.83, -185.09) Remaining time to fill dewars is 13140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 20 35 30, reference by 215 20 20 200 Disparity in stage position after aligning to target position: -0.12 0.02 (2nd move error 0.21 0.17) Finished aligning to item in 1 rounds Trimming A by 60 24 40 20, reference by 55 20 35 30 3.6 0.9 unbinned pixels; 1.3 14.5 nm along two shift axes; -4.4 13.9 nm on specimen axes Total_Shift = 7.502201 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.06 microns changed by -2.46 to target drift = 0.16 nm/sec Measured defocus = -2.37 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3416.tif 27 frames are being saved to U:\krios\20190717\PilA_3417.tif 27 frames are being saved to U:\krios\20190717\PilA_3418.tif 27 frames are being saved to U:\krios\20190717\PilA_3419.tif 27 frames are being saved to U:\krios\20190717\PilA_3420.tif 27 frames are being saved to U:\krios\20190717\PilA_3421.tif Running script 17 at item 642 (-731.15, -186.19) Remaining time to fill dewars is 12960 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 230 24 170 20, reference by 215 20 20 200 Trimming A by 230 24 170 20, reference by 220 24 165 20 Trimming A by 230 24 170 20, reference by 23 155 20 165 Trimming A by 230 24 170 20, reference by 24 145 160 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.20 Trimming A by 35 21 30 20, reference by 220 24 165 20 Disparity in stage position after aligning to target position: -0.38 -0.42 (2nd move error -0.00 -0.22) Finished aligning to item in 2 rounds Trimming A by 30 20 30 20, reference by 35 21 30 20 -27.0 2.2 unbinned pixels; 43.4 93.7 nm along two shift axes; -3.0 -105.3 nm on specimen axes Total_Shift = 54.08734 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.27 microns changed by -3.77 to target drift = 0.01 nm/sec Measured defocus = -2.58 microns changed by 0.08 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3422.tif 27 frames are being saved to U:\krios\20190717\PilA_3423.tif 27 frames are being saved to U:\krios\20190717\PilA_3424.tif 27 frames are being saved to U:\krios\20190717\PilA_3425.tif 27 frames are being saved to U:\krios\20190717\PilA_3426.tif 27 frames are being saved to U:\krios\20190717\PilA_3427.tif Running script 17 at item 643 (-728.54, -187.83) Remaining time to fill dewars is 12720 Dewars are NOT busy filling Trimming A by 24 137 175 20, reference by 215 20 20 200 Trimming A by 24 137 175 20, reference by 220 24 165 20 Trimming A by 24 137 175 20, reference by 23 155 20 165 Trimming A by 24 137 175 20, reference by 24 145 160 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.23 Trimming A by 21 21 60 20, reference by 24 145 160 20 Disparity in stage position after aligning to target position: -0.46 -0.40 (2nd move error -0.08 -0.20) Finished aligning to item in 2 rounds Trimming A by 23 25 60 20, reference by 21 21 60 20 -24.6 17.0 unbinned pixels; 94.5 63.0 nm along two shift axes; -60.8 -99.3 nm on specimen axes Total_Shift = 59.759852 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.21 microns changed by -2.39 to target drift = 0.01 nm/sec Measured defocus = -2.62 microns changed by 0.02 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3428.tif 27 frames are being saved to U:\krios\20190717\PilA_3429.tif 27 frames are being saved to U:\krios\20190717\PilA_3430.tif 27 frames are being saved to U:\krios\20190717\PilA_3431.tif 27 frames are being saved to U:\krios\20190717\PilA_3432.tif 27 frames are being saved to U:\krios\20190717\PilA_3433.tif Running script 17 at item 644 (-726.01, -189.62) Remaining time to fill dewars is 12480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 77 140 20, reference by 24 145 160 20 Disparity in stage position after aligning to target position: -0.41 -0.30 (2nd move error -0.04 -0.09) Finished aligning to item in 1 rounds Trimming A by 24 77 140 20, reference by 21 77 140 20 -14.1 12.0 unbinned pixels; 62.4 32.6 nm along two shift axes; -43.6 -57.2 nm on specimen axes Total_Shift = 36.943592 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.46 microns changed by -2.36 to target drift = 0.05 nm/sec Measured defocus = -0.88 microns changed by -0.02 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3434.tif 27 frames are being saved to U:\krios\20190717\PilA_3435.tif 27 frames are being saved to U:\krios\20190717\PilA_3436.tif 27 frames are being saved to U:\krios\20190717\PilA_3437.tif 27 frames are being saved to U:\krios\20190717\PilA_3438.tif 27 frames are being saved to U:\krios\20190717\PilA_3439.tif Running script 17 at item 645 (-727.63, -192.21) Remaining time to fill dewars is 12240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 101 120 20, reference by 24 145 160 20 Disparity in stage position after aligning to target position: -0.33 -0.35 (2nd move error 0.05 -0.15) Finished aligning to item in 1 rounds Trimming A by 21 97 125 20, reference by 22 101 120 20 -17.6 0.0 unbinned pixels; 23.0 63.4 nm along two shift axes; 3.7 -68.5 nm on specimen axes Total_Shift = 35.212555 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.85 microns changed by -3.85 to target drift = 0.05 nm/sec Measured defocus = -1.10 microns changed by 0.10 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3440.tif 27 frames are being saved to U:\krios\20190717\PilA_3441.tif 27 frames are being saved to U:\krios\20190717\PilA_3442.tif 27 frames are being saved to U:\krios\20190717\PilA_3443.tif 27 frames are being saved to U:\krios\20190717\PilA_3444.tif 27 frames are being saved to U:\krios\20190717\PilA_3445.tif Running script 17 at item 646 (-730.15, -190.50) Remaining time to fill dewars is 12180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 40 45 20, reference by 24 145 160 20 Disparity in stage position after aligning to target position: -0.41 -0.51 (2nd move error -0.04 -0.31) Finished aligning to item in 1 rounds Trimming A by 20 45 45 20, reference by 21 40 45 20 -30.8 9.7 unbinned pixels; 75.9 96.2 nm along two shift axes; -31.4 -121.8 nm on specimen axes Total_Shift = 64.556156 Trimming A by 20 50 45 20, reference by 21 40 45 20 0.8 5.5 unbinned pixels; 19.2 11.2 nm along two shift axes; -21.6 2.0 nm on specimen axes Total_Shift = 11.139478 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.84 microns changed by -3.94 to target drift = 0.08 nm/sec Measured defocus = -1.22 microns changed by 0.12 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3446.tif 27 frames are being saved to U:\krios\20190717\PilA_3447.tif 27 frames are being saved to U:\krios\20190717\PilA_3448.tif 27 frames are being saved to U:\krios\20190717\PilA_3449.tif 27 frames are being saved to U:\krios\20190717\PilA_3450.tif 27 frames are being saved to U:\krios\20190717\PilA_3451.tif Running script 17 at item 647 (-732.63, -188.71) Remaining time to fill dewars is 11880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 145 160 20 Disparity in stage position after aligning to target position: -0.51 -0.60 (2nd move error -0.14 -0.39) Finished aligning to item in 1 rounds -24.6 26.1 unbinned pixels; 128.2 49.3 nm along two shift axes; -96.4 -101.3 nm on specimen axes Total_Shift = 71.801001 0.5 16.4 unbinned pixels; 59.7 26.6 nm along two shift axes; -64.0 -1.5 nm on specimen axes Total_Shift = 32.854672 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.50 microns changed by -2.70 to target drift = 0.06 nm/sec Measured defocus = -1.25 microns changed by 0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3452.tif 27 frames are being saved to U:\krios\20190717\PilA_3453.tif 27 frames are being saved to U:\krios\20190717\PilA_3454.tif 27 frames are being saved to U:\krios\20190717\PilA_3455.tif 27 frames are being saved to U:\krios\20190717\PilA_3456.tif 27 frames are being saved to U:\krios\20190717\PilA_3457.tif Running script 17 at item 648 (-735.24, -187.60) Remaining time to fill dewars is 11700 Dewars are NOT busy filling Trimming A by 22 150 20 160, reference by 215 20 20 200 Trimming A by 22 150 20 160, reference by 220 24 165 20 Trimming A by 22 150 20 160, reference by 23 155 20 165 Trimming A by 22 150 20 160, reference by 24 145 160 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.20 Trimming A by 25 20 20 40, reference by 23 155 20 165 Disparity in stage position after aligning to target position: -0.28 0.00 (2nd move error 0.05 0.18) Finished aligning to item in 2 rounds Trimming A by 30 20 20 40, reference by 25 20 20 40 0.4 1.1 unbinned pixels; 3.4 3.2 nm along two shift axes; -4.3 1.5 nm on specimen axes Total_Shift = 2.319863 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.72 microns changed by -4.02 to target drift = 0.14 nm/sec Measured defocus = -1.38 microns changed by 0.08 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3458.tif 27 frames are being saved to U:\krios\20190717\PilA_3459.tif 27 frames are being saved to U:\krios\20190717\PilA_3460.tif 27 frames are being saved to U:\krios\20190717\PilA_3461.tif 27 frames are being saved to U:\krios\20190717\PilA_3462.tif 27 frames are being saved to U:\krios\20190717\PilA_3463.tif Running script 17 at item 649 (-738.01, -185.92) Remaining time to fill dewars is 11520 Dewars are NOT busy filling Trimming A by 220 23 20 195, reference by 215 20 20 200 Trimming A by 220 23 20 195, reference by 220 24 165 20 Trimming A by 220 23 20 195, reference by 23 155 20 165 Trimming A by 220 23 20 195, reference by 24 145 160 20 Disparity in stage position after aligning to center of map frame: -0.27 -0.20 Trimming A by 65 20 20 135, reference by 215 20 20 200 Disparity in stage position after aligning to target position: -0.16 -0.12 (2nd move error 0.10 0.05) Finished aligning to item in 2 rounds Trimming A by 77 24 20 125, reference by 65 20 20 135 -1.4 -2.1 unbinned pixels; 5.8 8.3 nm along two shift axes; 8.4 -5.1 nm on specimen axes Total_Shift = 5.054732 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.32 microns changed by -2.72 to target drift = 0.13 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3464.tif 27 frames are being saved to U:\krios\20190717\PilA_3465.tif 27 frames are being saved to U:\krios\20190717\PilA_3466.tif 27 frames are being saved to U:\krios\20190717\PilA_3467.tif 27 frames are being saved to U:\krios\20190717\PilA_3468.tif 27 frames are being saved to U:\krios\20190717\PilA_3469.tif Running script 17 at item 650 (-739.43, -188.46) Remaining time to fill dewars is 11340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 41 21 20 155, reference by 23 155 20 165 Disparity in stage position after aligning to target position: -0.38 -0.20 (2nd move error -0.03 -0.03) Finished aligning to item in 1 rounds Trimming A by 41 21 20 155, reference by 41 21 20 155 -5.4 6.5 unbinned pixels; 30.7 9.6 nm along two shift axes; -24.0 -22.2 nm on specimen axes Total_Shift = 16.766554 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.69 microns changed by -4.19 to target drift = 0.10 nm/sec Measured defocus = -1.54 microns changed by 0.04 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3470.tif 27 frames are being saved to U:\krios\20190717\PilA_3471.tif 27 frames are being saved to U:\krios\20190717\PilA_3472.tif 27 frames are being saved to U:\krios\20190717\PilA_3473.tif 27 frames are being saved to U:\krios\20190717\PilA_3474.tif 27 frames are being saved to U:\krios\20190717\PilA_3475.tif Running script 17 at item 651 (-736.86, -190.18) Remaining time to fill dewars is 11100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 55 20 70, reference by 23 155 20 165 Disparity in stage position after aligning to target position: -0.26 -0.10 (2nd move error 0.09 0.08) Finished aligning to item in 1 rounds Trimming A by 21 50 20 70, reference by 21 55 20 70 -0.0 -0.3 unbinned pixels; 1.2 0.7 nm along two shift axes; 1.3 -0.1 nm on specimen axes Total_Shift = 0.682669 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.14 microns changed by -2.74 to target drift = 0.16 nm/sec Measured defocus = -1.59 microns changed by -0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3476.tif 27 frames are being saved to U:\krios\20190717\PilA_3477.tif 27 frames are being saved to U:\krios\20190717\PilA_3478.tif 27 frames are being saved to U:\krios\20190717\PilA_3479.tif 27 frames are being saved to U:\krios\20190717\PilA_3480.tif 27 frames are being saved to U:\krios\20190717\PilA_3481.tif Running script 17 at item 652 (-734.37, -191.92) Remaining time to fill dewars is 10920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 60 20 65, reference by 23 155 20 165 Disparity in stage position after aligning to target position: -0.23 0.03 (2nd move error 0.11 0.21) Finished aligning to item in 1 rounds Trimming A by 21 55 20 60, reference by 20 60 20 65 1.1 -1.2 unbinned pixels; 6.0 2.1 nm along two shift axes; 4.6 4.6 nm on specimen axes Total_Shift = 3.34181 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 2.26 microns changed by -3.96 to target drift = 0.19 nm/sec Measured defocus = -1.71 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3482.tif 27 frames are being saved to U:\krios\20190717\PilA_3483.tif 27 frames are being saved to U:\krios\20190717\PilA_3484.tif 27 frames are being saved to U:\krios\20190717\PilA_3485.tif 27 frames are being saved to U:\krios\20190717\PilA_3486.tif 27 frames are being saved to U:\krios\20190717\PilA_3487.tif Running script 17 at item 653 (-731.76, -193.06) Remaining time to fill dewars is 10800 Dewars are NOT busy filling Trimming A by 24 137 175 20, reference by 215 20 20 200 Trimming A by 24 137 175 20, reference by 220 24 165 20 Trimming A by 24 137 175 20, reference by 23 155 20 165 Trimming A by 24 137 175 20, reference by 24 145 160 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.20 Trimming A by 20 60 40 45, reference by 24 145 160 20 Disparity in stage position after aligning to target position: -0.43 -0.51 (2nd move error -0.09 -0.33) Finished aligning to item in 2 rounds Trimming A by 20 65 40 50, reference by 20 60 40 45 -22.6 22.6 unbinned pixels; 112.6 47.4 nm along two shift axes; -83.1 -92.8 nm on specimen axes Total_Shift = 63.953091 Trimming A by 20 65 45 55, reference by 20 60 40 45 1.5 14.8 unbinned pixels; 52.4 27.7 nm along two shift axes; -57.8 2.8 nm on specimen axes Total_Shift = 29.713536 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.89 microns changed by -2.69 to target drift = 0.01 nm/sec Measured defocus = -1.86 microns changed by 0.06 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3488.tif 27 frames are being saved to U:\krios\20190717\PilA_3489.tif 27 frames are being saved to U:\krios\20190717\PilA_3490.tif 27 frames are being saved to U:\krios\20190717\PilA_3491.tif 27 frames are being saved to U:\krios\20190717\PilA_3492.tif 27 frames are being saved to U:\krios\20190717\PilA_3493.tif Running script 17 at item 654 (-729.30, -194.76) Remaining time to fill dewars is 10560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 130 95 20, reference by 24 145 160 20 Disparity in stage position after aligning to target position: -0.34 -0.39 (2nd move error -0.01 -0.21) Finished aligning to item in 1 rounds Trimming A by 24 117 115 20, reference by 24 130 95 20 -27.6 4.5 unbinned pixels; 52.6 92.6 nm along two shift axes; -11.7 -108.4 nm on specimen axes Total_Shift = 55.952772 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 2.01 microns changed by -3.91 to target drift = 0.01 nm/sec Measured defocus = -2.02 microns changed by 0.12 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3494.tif 27 frames are being saved to U:\krios\20190717\PilA_3495.tif 27 frames are being saved to U:\krios\20190717\PilA_3496.tif 27 frames are being saved to U:\krios\20190717\PilA_3497.tif 27 frames are being saved to U:\krios\20190717\PilA_3498.tif 27 frames are being saved to U:\krios\20190717\PilA_3499.tif Running script 17 at item 655 (-730.84, -197.37) Remaining time to fill dewars is 10380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 170 75 80, reference by 24 145 160 20 Disparity in stage position after aligning to target position: -0.25 -0.44 (2nd move error 0.09 -0.26) Finished aligning to item in 1 rounds Trimming A by 20 203 80 25, reference by 20 170 75 80 -24.2 -2.9 unbinned pixels; 21.0 91.3 nm along two shift axes; 16.2 -93.4 nm on specimen axes Total_Shift = 48.662694 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.63 microns changed by -2.63 to target drift = 0.13 nm/sec Measured defocus = -2.07 microns changed by 0.07 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3500.tif 27 frames are being saved to U:\krios\20190717\PilA_3501.tif 27 frames are being saved to U:\krios\20190717\PilA_3502.tif 27 frames are being saved to U:\krios\20190717\PilA_3503.tif 27 frames are being saved to U:\krios\20190717\PilA_3504.tif 27 frames are being saved to U:\krios\20190717\PilA_3505.tif Running script 17 at item 656 (-733.47, -196.15) Remaining time to fill dewars is 10140 Dewars are NOT busy filling Trimming A by 22 150 20 160, reference by 215 20 20 200 Trimming A by 22 150 20 160, reference by 220 24 165 20 Trimming A by 22 150 20 160, reference by 23 155 20 165 Trimming A by 22 150 20 160, reference by 24 145 160 20 Disparity in stage position after aligning to center of map frame: -0.34 -0.21 Trimming A by 20 150 20 115, reference by 23 155 20 165 Disparity in stage position after aligning to target position: -0.14 0.11 (2nd move error 0.19 0.30) Finished aligning to item in 2 rounds Trimming A by 24 145 20 105, reference by 20 150 20 115 3.1 6.4 unbinned pixels; 19.6 21.0 nm along two shift axes; -25.7 10.9 nm on specimen axes Total_Shift = 14.351028 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.84 microns changed by -3.94 to target drift = 0.17 nm/sec Measured defocus = -2.12 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3506.tif 27 frames are being saved to U:\krios\20190717\PilA_3507.tif 27 frames are being saved to U:\krios\20190717\PilA_3508.tif 27 frames are being saved to U:\krios\20190717\PilA_3509.tif 27 frames are being saved to U:\krios\20190717\PilA_3510.tif 27 frames are being saved to U:\krios\20190717\PilA_3511.tif Running script 17 at item 657 (-735.96, -194.50) Remaining time to fill dewars is 10020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 130 20 125, reference by 23 155 20 165 Disparity in stage position after aligning to target position: -0.26 -0.07 (2nd move error 0.07 0.12) Finished aligning to item in 1 rounds Trimming A by 20 130 20 120, reference by 23 130 20 125 0.6 -2.3 unbinned pixels; 9.4 1.4 nm along two shift axes; 9.0 2.8 nm on specimen axes Total_Shift = 4.820961 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.51 microns changed by -2.71 to target drift = 0.11 nm/sec Measured defocus = -2.23 microns changed by 0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3512.tif 27 frames are being saved to U:\krios\20190717\PilA_3513.tif 27 frames are being saved to U:\krios\20190717\PilA_3514.tif 27 frames are being saved to U:\krios\20190717\PilA_3515.tif 27 frames are being saved to U:\krios\20190717\PilA_3516.tif 27 frames are being saved to U:\krios\20190717\PilA_3517.tif Running script 17 at item 658 (-738.48, -192.77) Remaining time to fill dewars is 9780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 120 20 140, reference by 23 155 20 165 Disparity in stage position after aligning to target position: -0.31 -0.18 (2nd move error 0.02 0.01) Finished aligning to item in 1 rounds Trimming A by 24 120 20 140, reference by 24 120 20 140 1.8 -3.0 unbinned pixels; 13.4 1.8 nm along two shift axes; 11.3 7.5 nm on specimen axes Total_Shift = 6.990003 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.85 microns changed by -4.15 to target drift = 0.14 nm/sec Measured defocus = -2.36 microns changed by 0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3518.tif 27 frames are being saved to U:\krios\20190717\PilA_3519.tif 27 frames are being saved to U:\krios\20190717\PilA_3520.tif 27 frames are being saved to U:\krios\20190717\PilA_3521.tif 27 frames are being saved to U:\krios\20190717\PilA_3522.tif 27 frames are being saved to U:\krios\20190717\PilA_3523.tif Running script 17 at item 659 (-779.15, -155.71) Remaining time to fill dewars is 9600 Dewars are NOT busy filling Trimming A by 245 21 190 20, reference by 221 21 20 235 Trimming A by 245 21 190 20, reference by 230 21 180 20 Trimming A by 245 21 190 20, reference by 20 165 20 185 Trimming A by 245 21 190 20, reference by 23 165 160 20 Disparity in stage position after aligning to center of map frame: -0.44 -0.29 Trimming A by 91 21 175 20, reference by 230 21 180 20 Disparity in stage position after aligning to target position: -0.32 -0.18 (2nd move error 0.12 0.09) Finished aligning to item in 2 rounds Trimming A by 91 21 180 20, reference by 91 21 175 20 0.4 -1.5 unbinned pixels; 6.3 0.7 nm along two shift axes; 5.9 2.0 nm on specimen axes Total_Shift = 3.216474 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.05 microns changed by -2.45 to target drift = 0.12 nm/sec Measured defocus = -2.51 microns changed by 0.11 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3524.tif 27 frames are being saved to U:\krios\20190717\PilA_3525.tif 27 frames are being saved to U:\krios\20190717\PilA_3526.tif 27 frames are being saved to U:\krios\20190717\PilA_3527.tif 27 frames are being saved to U:\krios\20190717\PilA_3528.tif 27 frames are being saved to U:\krios\20190717\PilA_3529.tif Running script 17 at item 660 (-781.71, -154.04) Remaining time to fill dewars is 9420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 101 20 140 20, reference by 230 21 180 20 Disparity in stage position after aligning to target position: -0.31 -0.33 (2nd move error 0.12 -0.06) Finished aligning to item in 1 rounds Trimming A by 115 24 130 20, reference by 101 20 140 20 -5.7 -0.7 unbinned pixels; 4.8 21.8 nm along two shift axes; 4.1 -22.2 nm on specimen axes Total_Shift = 11.587764 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.32 microns changed by -3.82 to target drift = 0.09 nm/sec Measured defocus = -2.56 microns changed by 0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3530.tif 27 frames are being saved to U:\krios\20190717\PilA_3531.tif 27 frames are being saved to U:\krios\20190717\PilA_3532.tif 27 frames are being saved to U:\krios\20190717\PilA_3533.tif 27 frames are being saved to U:\krios\20190717\PilA_3534.tif 27 frames are being saved to U:\krios\20190717\PilA_3535.tif Running script 17 at item 661 (-784.16, -152.35) Remaining time to fill dewars is 9180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 24 110 20, reference by 230 21 180 20 Disparity in stage position after aligning to target position: -0.42 -0.41 (2nd move error 0.02 -0.14) Finished aligning to item in 1 rounds Trimming A by 130 24 105 20, reference by 130 24 110 20 -16.9 -0.6 unbinned pixels; 20.0 61.6 nm along two shift axes; 5.7 -65.5 nm on specimen axes Total_Shift = 33.759311 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.02 microns changed by -2.62 to target drift = 0.10 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3536.tif 27 frames are being saved to U:\krios\20190717\PilA_3537.tif 27 frames are being saved to U:\krios\20190717\PilA_3538.tif 27 frames are being saved to U:\krios\20190717\PilA_3539.tif 27 frames are being saved to U:\krios\20190717\PilA_3540.tif 27 frames are being saved to U:\krios\20190717\PilA_3541.tif Running script 17 at item 662 (-786.68, -150.63) Remaining time to fill dewars is 9120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 20 90 30, reference by 230 21 180 20 Disparity in stage position after aligning to target position: -0.51 -0.47 (2nd move error -0.08 -0.21) Finished aligning to item in 1 rounds Trimming A by 150 21 90 25, reference by 150 20 90 30 -24.6 17.5 unbinned pixels; 96.3 62.3 nm along two shift axes; -62.7 -99.4 nm on specimen axes Total_Shift = 60.330403 Trimming A by 145 24 90 30, reference by 150 20 90 30 -4.5 10.7 unbinned pixels; 45.1 0.2 nm along two shift axes; -40.5 -19.8 nm on specimen axes Total_Shift = 23.14059 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.91 microns changed by -2.81 to target drift = 0.04 nm/sec Measured defocus = -0.90 microns changed by -0.00 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3542.tif 27 frames are being saved to U:\krios\20190717\PilA_3543.tif 27 frames are being saved to U:\krios\20190717\PilA_3544.tif 27 frames are being saved to U:\krios\20190717\PilA_3545.tif 27 frames are being saved to U:\krios\20190717\PilA_3546.tif 27 frames are being saved to U:\krios\20190717\PilA_3547.tif Running script 17 at item 663 (-790.98, -152.05) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 235 24 20 220, reference by 221 21 20 235 Trimming A by 235 24 20 220, reference by 230 21 180 20 Trimming A by 235 24 20 220, reference by 20 165 20 185 Trimming A by 235 24 20 220, reference by 23 165 160 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.20 Trimming A by 155 23 20 135, reference by 221 21 20 235 Disparity in stage position after aligning to target position: -0.20 -0.11 (2nd move error 0.13 0.07) Finished aligning to item in 2 rounds Trimming A by 150 20 20 135, reference by 155 23 20 135 -0.2 5.1 unbinned pixels; 19.1 6.8 nm along two shift axes; -19.9 -2.0 nm on specimen axes Total_Shift = 10.241603 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 3.28 microns changed by -4.28 to target drift = 0.18 nm/sec Measured defocus = -1.06 microns changed by 0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3548.tif 27 frames are being saved to U:\krios\20190717\PilA_3549.tif 27 frames are being saved to U:\krios\20190717\PilA_3550.tif 27 frames are being saved to U:\krios\20190717\PilA_3551.tif 27 frames are being saved to U:\krios\20190717\PilA_3552.tif 27 frames are being saved to U:\krios\20190717\PilA_3553.tif Running script 17 at item 664 (-788.49, -153.74) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 85 24 20 70, reference by 221 21 20 235 Disparity in stage position after aligning to target position: -0.12 0.03 (2nd move error 0.21 0.21) Finished aligning to item in 1 rounds Trimming A by 85 24 20 70, reference by 85 24 20 70 2.4 -4.1 unbinned pixels; 18.1 2.4 nm along two shift axes; 15.4 10.1 nm on specimen axes Total_Shift = 9.439657 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.70 microns changed by -2.80 to target drift = 0.17 nm/sec Measured defocus = -1.11 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3554.tif 27 frames are being saved to U:\krios\20190717\PilA_3555.tif 27 frames are being saved to U:\krios\20190717\PilA_3556.tif 27 frames are being saved to U:\krios\20190717\PilA_3557.tif 27 frames are being saved to U:\krios\20190717\PilA_3558.tif 27 frames are being saved to U:\krios\20190717\PilA_3559.tif Running script 17 at item 665 (-785.83, -154.92) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 239 20 190 20, reference by 221 21 20 235 Trimming A by 239 20 190 20, reference by 230 21 180 20 Trimming A by 239 20 190 20, reference by 20 165 20 185 Trimming A by 239 20 190 20, reference by 23 165 160 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.22 Trimming A by 55 20 45 20, reference by 230 21 180 20 Disparity in stage position after aligning to target position: -0.36 -0.42 (2nd move error -0.00 -0.23) Finished aligning to item in 2 rounds Trimming A by 55 20 45 20, reference by 55 20 45 20 -26.1 3.2 unbinned pixels; 45.6 89.1 nm along two shift axes; -6.8 -102.1 nm on specimen axes Total_Shift = 52.517555 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.88 microns changed by -4.08 to target drift = 0.05 nm/sec Measured defocus = -1.27 microns changed by 0.07 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3560.tif 27 frames are being saved to U:\krios\20190717\PilA_3561.tif 27 frames are being saved to U:\krios\20190717\PilA_3562.tif 27 frames are being saved to U:\krios\20190717\PilA_3563.tif 27 frames are being saved to U:\krios\20190717\PilA_3564.tif 27 frames are being saved to U:\krios\20190717\PilA_3565.tif Running script 17 at item 666 (-783.17, -156.63) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 23 160 175 20, reference by 221 21 20 235 Trimming A by 23 160 175 20, reference by 230 21 180 20 Trimming A by 23 160 175 20, reference by 20 165 20 185 Trimming A by 23 160 175 20, reference by 23 165 160 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.25 Trimming A by 50 21 65 20, reference by 23 165 160 20 Disparity in stage position after aligning to target position: -0.43 -0.41 (2nd move error -0.07 -0.18) Finished aligning to item in 2 rounds Trimming A by 45 21 65 20, reference by 50 21 65 20 -18.6 22.8 unbinned pixels; 108.0 32.6 nm along two shift axes; -84.7 -77.1 nm on specimen axes Total_Shift = 58.787095 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.20 microns changed by -2.50 to target drift = 0.04 nm/sec Measured defocus = -1.24 microns changed by -0.06 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3566.tif 27 frames are being saved to U:\krios\20190717\PilA_3567.tif 27 frames are being saved to U:\krios\20190717\PilA_3568.tif 27 frames are being saved to U:\krios\20190717\PilA_3569.tif 27 frames are being saved to U:\krios\20190717\PilA_3570.tif 27 frames are being saved to U:\krios\20190717\PilA_3571.tif Running script 17 at item 667 (-780.69, -158.29) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 85 120 20, reference by 23 165 160 20 Disparity in stage position after aligning to target position: -0.41 -0.30 (2nd move error -0.04 -0.07) Finished aligning to item in 1 rounds Trimming A by 21 85 120 20, reference by 21 85 120 20 -8.8 9.4 unbinned pixels; 46.2 17.4 nm along two shift axes; -34.9 -36.1 nm on specimen axes Total_Shift = 25.770808 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.36 microns changed by -3.76 to target drift = 0.08 nm/sec Measured defocus = -1.39 microns changed by -0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3572.tif 27 frames are being saved to U:\krios\20190717\PilA_3573.tif 27 frames are being saved to U:\krios\20190717\PilA_3574.tif 27 frames are being saved to U:\krios\20190717\PilA_3575.tif 27 frames are being saved to U:\krios\20190717\PilA_3576.tif 27 frames are being saved to U:\krios\20190717\PilA_3577.tif Running script 17 at item 668 (-782.32, -160.96) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 110 105 20, reference by 23 165 160 20 Disparity in stage position after aligning to target position: -0.28 -0.40 (2nd move error 0.09 -0.17) Finished aligning to item in 1 rounds Trimming A by 22 110 105 20, reference by 22 110 105 20 -15.4 -2.3 unbinned pixels; 11.7 58.8 nm along two shift axes; 12.1 -59.3 nm on specimen axes Total_Shift = 31.089651 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.98 microns changed by -2.48 to target drift = 0.11 nm/sec Measured defocus = -1.48 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3578.tif 27 frames are being saved to U:\krios\20190717\PilA_3579.tif 27 frames are being saved to U:\krios\20190717\PilA_3580.tif 27 frames are being saved to U:\krios\20190717\PilA_3581.tif 27 frames are being saved to U:\krios\20190717\PilA_3582.tif 27 frames are being saved to U:\krios\20190717\PilA_3583.tif Running script 17 at item 669 (-790.07, -156.29) Remaining time to fill dewars is 8880 Dewars are NOT busy filling Trimming A by 235 24 20 220, reference by 221 21 20 235 Trimming A by 235 24 20 220, reference by 230 21 180 20 Trimming A by 235 24 20 220, reference by 20 165 20 185 Trimming A by 235 24 20 220, reference by 23 165 160 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.22 Trimming A by 51 20 20 85, reference by 221 21 20 235 Disparity in stage position after aligning to target position: -0.20 -0.09 (2nd move error 0.15 0.10) Finished aligning to item in 2 rounds Trimming A by 46 21 20 85, reference by 51 20 20 85 -0.3 -4.6 unbinned pixels; 16.7 8.1 nm along two shift axes; 18.1 -0.2 nm on specimen axes Total_Shift = 9.31088 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.55 microns changed by -4.15 to target drift = 0.17 nm/sec Measured defocus = -1.60 microns changed by 0.00 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3584.tif 27 frames are being saved to U:\krios\20190717\PilA_3585.tif 27 frames are being saved to U:\krios\20190717\PilA_3586.tif 27 frames are being saved to U:\krios\20190717\PilA_3587.tif 27 frames are being saved to U:\krios\20190717\PilA_3588.tif 27 frames are being saved to U:\krios\20190717\PilA_3589.tif Running script 17 at item 670 (-792.55, -154.64) Remaining time to fill dewars is 8880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 111 22 45 160, reference by 221 21 20 235 Disparity in stage position after aligning to target position: -0.22 -0.19 (2nd move error 0.13 0.01) Finished aligning to item in 1 rounds Trimming A by 99 22 50 165, reference by 111 22 45 160 1.6 -4.5 unbinned pixels; 18.7 0.9 nm along two shift axes; 17.2 7.3 nm on specimen axes Total_Shift = 9.608915 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.22 microns changed by -2.92 to target drift = 0.13 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3590.tif 27 frames are being saved to U:\krios\20190717\PilA_3591.tif 27 frames are being saved to U:\krios\20190717\PilA_3592.tif 27 frames are being saved to U:\krios\20190717\PilA_3593.tif 27 frames are being saved to U:\krios\20190717\PilA_3594.tif 27 frames are being saved to U:\krios\20190717\PilA_3595.tif Running script 17 at item 671 (-793.99, -157.10) Remaining time to fill dewars is 7620 Dewars are NOT busy filling Trimming A by 20 155 20 180, reference by 221 21 20 235 Trimming A by 20 155 20 180, reference by 230 21 180 20 Trimming A by 20 155 20 180, reference by 20 165 20 185 Trimming A by 20 155 20 180, reference by 23 165 160 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.23 Trimming A by 74 22 20 185, reference by 20 165 20 185 Disparity in stage position after aligning to target position: -0.42 -0.27 (2nd move error -0.06 -0.06) Finished aligning to item in 2 rounds Trimming A by 56 22 20 195, reference by 74 22 20 185 -8.9 20.9 unbinned pixels; 88.2 0.5 nm along two shift axes; -79.3 -38.9 nm on specimen axes Total_Shift = 45.315433 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 2.64 microns changed by -4.43 to target drift = 0.14 nm/sec Measured defocus = -1.92 microns changed by 0.12 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3596.tif 27 frames are being saved to U:\krios\20190717\PilA_3597.tif 27 frames are being saved to U:\krios\20190717\PilA_3598.tif 27 frames are being saved to U:\krios\20190717\PilA_3599.tif 27 frames are being saved to U:\krios\20190717\PilA_3600.tif 27 frames are being saved to U:\krios\20190717\PilA_3601.tif Running script 17 at item 672 (-791.47, -158.89) Remaining time to fill dewars is 7500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 55 20 85, reference by 20 165 20 185 Disparity in stage position after aligning to target position: -0.30 -0.18 (2nd move error 0.05 0.04) Finished aligning to item in 1 rounds Trimming A by 26 37 20 100, reference by 55 55 20 85 -1.3 -0.1 unbinned pixels; 1.4 4.7 nm along two shift axes; 0.5 -5.0 nm on specimen axes Total_Shift = 2.586028 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.09 microns changed by -2.99 to target drift = 0.13 nm/sec Measured defocus = -1.99 microns changed by 0.09 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3602.tif 27 frames are being saved to U:\krios\20190717\PilA_3603.tif 27 frames are being saved to U:\krios\20190717\PilA_3604.tif 27 frames are being saved to U:\krios\20190717\PilA_3605.tif 27 frames are being saved to U:\krios\20190717\PilA_3606.tif 27 frames are being saved to U:\krios\20190717\PilA_3607.tif Running script 17 at item 673 (-788.95, -160.61) Remaining time to fill dewars is 7260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 65 20 70, reference by 20 165 20 185 Disparity in stage position after aligning to target position: -0.27 -0.09 (2nd move error 0.09 0.12) Finished aligning to item in 1 rounds Trimming A by 20 60 20 65, reference by 20 65 20 70 0.7 -2.1 unbinned pixels; 8.5 0.5 nm along two shift axes; 7.8 3.3 nm on specimen axes Total_Shift = 4.359096 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 2.21 microns changed by -4.21 to target drift = 0.16 nm/sec Measured defocus = -2.10 microns changed by 0.10 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3608.tif 27 frames are being saved to U:\krios\20190717\PilA_3609.tif 27 frames are being saved to U:\krios\20190717\PilA_3610.tif 27 frames are being saved to U:\krios\20190717\PilA_3611.tif 27 frames are being saved to U:\krios\20190717\PilA_3612.tif 27 frames are being saved to U:\krios\20190717\PilA_3613.tif Running script 17 at item 674 (-786.46, -161.85) Remaining time to fill dewars is 7140 Dewars are NOT busy filling Trimming A by 23 160 175 20, reference by 221 21 20 235 Trimming A by 23 160 175 20, reference by 230 21 180 20 Trimming A by 23 160 175 20, reference by 20 165 20 185 Trimming A by 23 160 175 20, reference by 23 165 160 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.23 Trimming A by 21 65 40 45, reference by 23 165 160 20 Disparity in stage position after aligning to target position: -0.40 -0.50 (2nd move error -0.07 -0.29) Finished aligning to item in 2 rounds Trimming A by 20 70 40 50, reference by 21 65 40 45 -28.3 27.0 unbinned pixels; 136.2 61.1 nm along two shift axes; -99.2 -115.6 nm on specimen axes Total_Shift = 78.196844 Trimming A by 21 75 45 50, reference by 21 65 40 45 1.7 17.7 unbinned pixels; 62.7 32.7 nm along two shift axes; -69.1 2.9 nm on specimen axes Total_Shift = 35.496536 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.65 microns changed by -2.75 to target drift = 0.02 nm/sec Measured defocus = -2.07 microns changed by -0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3614.tif 27 frames are being saved to U:\krios\20190717\PilA_3615.tif 27 frames are being saved to U:\krios\20190717\PilA_3616.tif 27 frames are being saved to U:\krios\20190717\PilA_3617.tif 27 frames are being saved to U:\krios\20190717\PilA_3618.tif 27 frames are being saved to U:\krios\20190717\PilA_3619.tif Running script 17 at item 675 (-790.62, -163.22) Remaining time to fill dewars is 6900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 140 70 125, reference by 20 165 20 185 Disparity in stage position after aligning to target position: -0.31 -0.14 (2nd move error 0.04 0.07) Finished aligning to item in 1 rounds Trimming A by 22 135 20 130, reference by 21 140 70 125 -4.7 -1.7 unbinned pixels; 0.0 19.5 nm along two shift axes; 7.5 -18.0 nm on specimen axes Total_Shift = 10.000444 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 2.01 microns changed by -4.21 to target drift = 0.11 nm/sec Measured defocus = -2.19 microns changed by -0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3620.tif 27 frames are being saved to U:\krios\20190717\PilA_3621.tif 27 frames are being saved to U:\krios\20190717\PilA_3622.tif 27 frames are being saved to U:\krios\20190717\PilA_3623.tif 27 frames are being saved to U:\krios\20190717\PilA_3624.tif 27 frames are being saved to U:\krios\20190717\PilA_3625.tif Running script 17 at item 676 (-717.87, -42.67) Remaining time to fill dewars is 6780 Dewars are NOT busy filling Trimming A by 249 20 185 20, reference by 235 20 20 230 Trimming A by 249 20 185 20, reference by 240 24 165 20 Trimming A by 249 20 185 20, reference by 20 140 20 165 Trimming A by 249 20 185 20, reference by 23 125 125 20 Disparity in stage position after aligning to center of map frame: -0.50 -0.24 Trimming A by 130 22 195 20, reference by 240 24 165 20 Disparity in stage position after aligning to target position: -0.36 -0.11 (2nd move error 0.14 0.11) Finished aligning to item in 2 rounds Trimming A by 135 23 200 20, reference by 130 22 195 20 0.3 -2.2 unbinned pixels; 8.4 2.0 nm along two shift axes; 8.3 1.8 nm on specimen axes Total_Shift = 4.382119 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Using peak at -6.8,-7.0, rejecting 1.12 times higher peak at -0.3,0.3 Measured defocus = 0.21 microns changed by -2.51 to target drift = 0.64 nm/sec Measured defocus = -2.61 microns changed by 0.31 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3626.tif 27 frames are being saved to U:\krios\20190717\PilA_3627.tif 27 frames are being saved to U:\krios\20190717\PilA_3628.tif 27 frames are being saved to U:\krios\20190717\PilA_3629.tif 27 frames are being saved to U:\krios\20190717\PilA_3630.tif 27 frames are being saved to U:\krios\20190717\PilA_3631.tif Running script 17 at item 677 (-720.41, -41.04) Remaining time to fill dewars is 6540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 22 155 20, reference by 240 24 165 20 Disparity in stage position after aligning to target position: -0.46 -0.25 (2nd move error 0.03 -0.03) Finished aligning to item in 1 rounds Trimming A by 150 22 155 20, reference by 150 22 155 20 -5.0 0.2 unbinned pixels; 7.3 17.8 nm along two shift axes; 0.3 -19.7 nm on specimen axes Total_Shift = 10.090886 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.20 microns changed by -3.60 to target drift = 0.07 nm/sec Measured defocus = -2.41 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3632.tif 27 frames are being saved to U:\krios\20190717\PilA_3633.tif 27 frames are being saved to U:\krios\20190717\PilA_3634.tif 27 frames are being saved to U:\krios\20190717\PilA_3635.tif 27 frames are being saved to U:\krios\20190717\PilA_3636.tif 27 frames are being saved to U:\krios\20190717\PilA_3637.tif Running script 17 at item 678 (-722.90, -39.40) Remaining time to fill dewars is 6360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 170 23 130 20, reference by 240 24 165 20 Disparity in stage position after aligning to target position: -0.46 -0.33 (2nd move error 0.04 -0.12) Finished aligning to item in 1 rounds Trimming A by 165 22 135 20, reference by 170 23 130 20 -14.9 -1.3 unbinned pixels; 14.9 55.6 nm along two shift axes; 8.0 -57.8 nm on specimen axes Total_Shift = 29.97134 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.07 microns changed by -2.43 to target drift = 0.09 nm/sec Measured defocus = -2.53 microns changed by 0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3638.tif 27 frames are being saved to U:\krios\20190717\PilA_3639.tif 27 frames are being saved to U:\krios\20190717\PilA_3640.tif 27 frames are being saved to U:\krios\20190717\PilA_3641.tif 27 frames are being saved to U:\krios\20190717\PilA_3642.tif 27 frames are being saved to U:\krios\20190717\PilA_3643.tif Running script 17 at item 679 (-725.41, -37.77) Remaining time to fill dewars is 6240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 180 22 115 20, reference by 240 24 165 20 Disparity in stage position after aligning to target position: -0.42 -0.40 (2nd move error 0.07 -0.17) Finished aligning to item in 1 rounds Trimming A by 180 22 115 20, reference by 180 22 115 20 -21.9 -1.0 unbinned pixels; 24.9 80.3 nm along two shift axes; 8.5 -84.9 nm on specimen axes Total_Shift = 43.810425 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.03 microns changed by -2.57 to target drift = 0.10 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3644.tif 27 frames are being saved to U:\krios\20190717\PilA_3645.tif 27 frames are being saved to U:\krios\20190717\PilA_3646.tif 27 frames are being saved to U:\krios\20190717\PilA_3647.tif 27 frames are being saved to U:\krios\20190717\PilA_3648.tif 27 frames are being saved to U:\krios\20190717\PilA_3649.tif Running script 17 at item 680 (-729.74, -39.05) Remaining time to fill dewars is 6060 Dewars are NOT busy filling Trimming A by 240 24 20 220, reference by 235 20 20 230 Trimming A by 240 24 20 220, reference by 240 24 165 20 Trimming A by 240 24 20 220, reference by 20 140 20 165 Trimming A by 240 24 20 220, reference by 23 125 125 20 Disparity in stage position after aligning to center of map frame: -0.34 -0.16 Trimming A by 150 20 20 110, reference by 235 20 20 230 Disparity in stage position after aligning to target position: -0.18 0.04 (2nd move error 0.16 0.17) Finished aligning to item in 2 rounds Trimming A by 150 24 20 110, reference by 150 20 20 110 2.1 3.2 unbinned pixels; 9.0 12.4 nm along two shift axes; -12.8 7.6 nm on specimen axes Total_Shift = 7.652093 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.80 microns changed by -2.70 to target drift = 0.10 nm/sec Measured defocus = -0.87 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3650.tif 27 frames are being saved to U:\krios\20190717\PilA_3651.tif 27 frames are being saved to U:\krios\20190717\PilA_3652.tif 27 frames are being saved to U:\krios\20190717\PilA_3653.tif 27 frames are being saved to U:\krios\20190717\PilA_3654.tif 27 frames are being saved to U:\krios\20190717\PilA_3655.tif Running script 17 at item 681 (-727.03, -40.26) Remaining time to fill dewars is 5820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 100 20 65 35, reference by 240 24 165 20 Disparity in stage position after aligning to target position: -0.46 -0.46 (2nd move error 0.04 -0.24) Finished aligning to item in 1 rounds Trimming A by 95 20 65 35, reference by 100 20 65 35 -26.9 0.8 unbinned pixels; 38.0 95.7 nm along two shift axes; 2.6 -104.8 nm on specimen axes Total_Shift = 53.81357 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.97 microns changed by -3.97 to target drift = 0.07 nm/sec Measured defocus = -1.03 microns changed by 0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3656.tif 27 frames are being saved to U:\krios\20190717\PilA_3657.tif 27 frames are being saved to U:\krios\20190717\PilA_3658.tif 27 frames are being saved to U:\krios\20190717\PilA_3659.tif 27 frames are being saved to U:\krios\20190717\PilA_3660.tif 27 frames are being saved to U:\krios\20190717\PilA_3661.tif Running script 17 at item 682 (-724.52, -41.94) Remaining time to fill dewars is 5640 Dewars are NOT busy filling Trimming A by 250 24 175 20, reference by 235 20 20 230 Trimming A by 250 24 175 20, reference by 240 24 165 20 Trimming A by 250 24 175 20, reference by 20 140 20 165 Trimming A by 250 24 175 20, reference by 23 125 125 20 Disparity in stage position after aligning to center of map frame: -0.39 -0.17 Trimming A by 90 23 75 20, reference by 240 24 165 20 Disparity in stage position after aligning to target position: -0.43 -0.33 (2nd move error -0.04 -0.18) Finished aligning to item in 2 rounds Trimming A by 85 23 75 20, reference by 90 23 75 20 -24.8 9.2 unbinned pixels; 66.2 75.5 nm along two shift axes; -30.6 -98.5 nm on specimen axes Total_Shift = 52.944582 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.52 microns changed by -2.62 to target drift = 0.02 nm/sec Measured defocus = -1.16 microns changed by 0.06 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3662.tif 27 frames are being saved to U:\krios\20190717\PilA_3663.tif 27 frames are being saved to U:\krios\20190717\PilA_3664.tif 27 frames are being saved to U:\krios\20190717\PilA_3665.tif 27 frames are being saved to U:\krios\20190717\PilA_3666.tif 27 frames are being saved to U:\krios\20190717\PilA_3667.tif Running script 17 at item 683 (-722.01, -43.54) Remaining time to fill dewars is 5400 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 80 20 90 20, reference by 240 24 165 20 Disparity in stage position after aligning to target position: -0.29 -0.27 (2nd move error 0.09 -0.13) Finished aligning to item in 1 rounds Trimming A by 80 20 90 20, reference by 80 20 90 20 -15.7 -0.8 unbinned pixels; 17.4 57.9 nm along two shift axes; 6.6 -61.1 nm on specimen axes Total_Shift = 31.529036 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.57 microns changed by -3.77 to target drift = 0.09 nm/sec Measured defocus = -1.25 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3668.tif 27 frames are being saved to U:\krios\20190717\PilA_3669.tif 27 frames are being saved to U:\krios\20190717\PilA_3670.tif 27 frames are being saved to U:\krios\20190717\PilA_3671.tif 27 frames are being saved to U:\krios\20190717\PilA_3672.tif 27 frames are being saved to U:\krios\20190717\PilA_3673.tif Running script 17 at item 684 (-719.27, -45.31) Remaining time to fill dewars is 5220 Dewars are NOT busy filling Trimming A by 21 120 140 20, reference by 235 20 20 230 Trimming A by 21 120 140 20, reference by 240 24 165 20 Trimming A by 21 120 140 20, reference by 20 140 20 165 Trimming A by 21 120 140 20, reference by 23 125 125 20 Disparity in stage position after aligning to center of map frame: -0.44 -0.24 Trimming A by 45 51 145 20, reference by 23 125 125 20 Disparity in stage position after aligning to target position: -0.47 -0.17 (2nd move error -0.04 0.04) Finished aligning to item in 2 rounds Trimming A by 51 51 145 20, reference by 45 51 145 20 -7.3 24.5 unbinned pixels; 99.6 10.7 nm along two shift axes; -93.8 -33.4 nm on specimen axes Total_Shift = 51.121329 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.05 microns changed by -2.35 to target drift = 0.08 nm/sec Measured defocus = -1.29 microns changed by -0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3674.tif 27 frames are being saved to U:\krios\20190717\PilA_3675.tif 27 frames are being saved to U:\krios\20190717\PilA_3676.tif 27 frames are being saved to U:\krios\20190717\PilA_3677.tif 27 frames are being saved to U:\krios\20190717\PilA_3678.tif 27 frames are being saved to U:\krios\20190717\PilA_3679.tif Running script 17 at item 685 (-720.93, -47.89) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 50 135 20, reference by 23 125 125 20 Disparity in stage position after aligning to target position: -0.43 -0.25 (2nd move error -0.00 -0.03) Finished aligning to item in 1 rounds Trimming A by 22 55 135 20, reference by 20 50 135 20 -6.0 1.2 unbinned pixels; 12.3 20.0 nm along two shift axes; -3.4 -23.8 nm on specimen axes Total_Shift = 12.320038 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.27 microns changed by -3.67 to target drift = 0.06 nm/sec Measured defocus = -1.40 microns changed by -0.00 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3680.tif 27 frames are being saved to U:\krios\20190717\PilA_3681.tif 27 frames are being saved to U:\krios\20190717\PilA_3682.tif 27 frames are being saved to U:\krios\20190717\PilA_3683.tif 27 frames are being saved to U:\krios\20190717\PilA_3684.tif 27 frames are being saved to U:\krios\20190717\PilA_3685.tif Running script 17 at item 686 (-723.45, -46.22) Remaining time to fill dewars is 4860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 25 45 20, reference by 23 125 125 20 Disparity in stage position after aligning to target position: -0.49 -0.36 (2nd move error -0.07 -0.14) Finished aligning to item in 1 rounds Trimming A by 22 25 45 20, reference by 22 25 45 20 -17.2 11.9 unbinned pixels; 66.4 44.1 nm along two shift axes; -42.8 -69.6 nm on specimen axes Total_Shift = 41.962588 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.89 microns changed by -2.39 to target drift = 0.05 nm/sec Measured defocus = -1.50 microns changed by 0.00 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3686.tif 27 frames are being saved to U:\krios\20190717\PilA_3687.tif 27 frames are being saved to U:\krios\20190717\PilA_3688.tif 27 frames are being saved to U:\krios\20190717\PilA_3689.tif 27 frames are being saved to U:\krios\20190717\PilA_3690.tif 27 frames are being saved to U:\krios\20190717\PilA_3691.tif Running script 17 at item 687 (-725.99, -44.45) Remaining time to fill dewars is 4620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 125 125 20 Disparity in stage position after aligning to target position: -0.53 -0.49 (2nd move error -0.11 -0.27) Finished aligning to item in 1 rounds -30.3 26.4 unbinned pixels; 136.8 69.3 nm along two shift axes; -96.5 -123.5 nm on specimen axes Total_Shift = 80.442323 -0.3 15.7 unbinned pixels; 58.0 22.6 nm along two shift axes; -60.9 -4.3 nm on specimen axes Total_Shift = 31.352538 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.33 microns changed by -3.93 to target drift = 0.06 nm/sec Measured defocus = -1.67 microns changed by 0.07 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3692.tif 27 frames are being saved to U:\krios\20190717\PilA_3693.tif 27 frames are being saved to U:\krios\20190717\PilA_3694.tif 27 frames are being saved to U:\krios\20190717\PilA_3695.tif 27 frames are being saved to U:\krios\20190717\PilA_3696.tif 27 frames are being saved to U:\krios\20190717\PilA_3697.tif Running script 17 at item 688 (-728.87, -43.28) Remaining time to fill dewars is 4560 Dewars are NOT busy filling Trimming A by 235 21 20 225, reference by 235 20 20 230 Trimming A by 235 21 20 225, reference by 240 24 165 20 Trimming A by 235 21 20 225, reference by 20 140 20 165 Trimming A by 235 21 20 225, reference by 23 125 125 20 Disparity in stage position after aligning to center of map frame: -0.33 -0.16 Trimming A by 55 20 20 55, reference by 235 20 20 230 Disparity in stage position after aligning to target position: -0.11 0.04 (2nd move error 0.21 0.19) Finished aligning to item in 2 rounds Trimming A by 45 22 20 60, reference by 55 20 20 55 0.4 -3.5 unbinned pixels; 13.5 4.0 nm along two shift axes; 13.7 2.2 nm on specimen axes Total_Shift = 7.129795 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.88 microns changed by -2.58 to target drift = 0.14 nm/sec Measured defocus = -1.68 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3698.tif 27 frames are being saved to U:\krios\20190717\PilA_3699.tif 27 frames are being saved to U:\krios\20190717\PilA_3700.tif 27 frames are being saved to U:\krios\20190717\PilA_3701.tif 27 frames are being saved to U:\krios\20190717\PilA_3702.tif 27 frames are being saved to U:\krios\20190717\PilA_3703.tif Running script 17 at item 689 (-731.38, -41.66) Remaining time to fill dewars is 4320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 21 20 130, reference by 235 20 20 230 Disparity in stage position after aligning to target position: -0.17 -0.11 (2nd move error 0.15 0.03) Finished aligning to item in 1 rounds Trimming A by 111 24 20 130, reference by 115 21 20 130 -1.1 -6.6 unbinned pixels; 22.8 13.8 nm along two shift axes; 25.8 -2.9 nm on specimen axes Total_Shift = 13.329366 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 2.18 microns changed by -3.98 to target drift = 0.14 nm/sec Measured defocus = -1.84 microns changed by 0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3704.tif 27 frames are being saved to U:\krios\20190717\PilA_3705.tif 27 frames are being saved to U:\krios\20190717\PilA_3706.tif 27 frames are being saved to U:\krios\20190717\PilA_3707.tif 27 frames are being saved to U:\krios\20190717\PilA_3708.tif 27 frames are being saved to U:\krios\20190717\PilA_3709.tif Running script 17 at item 690 (-732.72, -44.25) Remaining time to fill dewars is 4140 Dewars are NOT busy filling Trimming A by 20 130 20 165, reference by 235 20 20 230 Trimming A by 20 130 20 165, reference by 240 24 165 20 Trimming A by 20 130 20 165, reference by 20 140 20 165 Trimming A by 20 130 20 165, reference by 23 125 125 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.18 Trimming A by 69 24 20 150, reference by 20 140 20 165 Disparity in stage position after aligning to target position: -0.41 -0.15 (2nd move error -0.11 0.01) Finished aligning to item in 2 rounds Trimming A by 69 24 20 150, reference by 69 24 20 150 2.7 28.6 unbinned pixels; 101.4 52.8 nm along two shift axes; -111.7 4.6 nm on specimen axes Total_Shift = 57.372269 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.86 microns changed by -2.76 to target drift = 0.14 nm/sec Measured defocus = -1.94 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3710.tif 27 frames are being saved to U:\krios\20190717\PilA_3711.tif 27 frames are being saved to U:\krios\20190717\PilA_3712.tif 27 frames are being saved to U:\krios\20190717\PilA_3713.tif 27 frames are being saved to U:\krios\20190717\PilA_3714.tif 27 frames are being saved to U:\krios\20190717\PilA_3715.tif Running script 17 at item 691 (-730.25, -45.94) Remaining time to fill dewars is 3960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 21 20 60, reference by 20 140 20 165 Disparity in stage position after aligning to target position: -0.29 -0.04 (2nd move error 0.01 0.12) Finished aligning to item in 1 rounds Trimming A by 30 20 20 65, reference by 35 21 20 60 -2.0 -2.1 unbinned pixels; 5.2 10.5 nm along two shift axes; 8.7 -7.4 nm on specimen axes Total_Shift = 5.868875 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.91 microns changed by -3.91 to target drift = 0.16 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3716.tif 27 frames are being saved to U:\krios\20190717\PilA_3717.tif 27 frames are being saved to U:\krios\20190717\PilA_3718.tif 27 frames are being saved to U:\krios\20190717\PilA_3719.tif 27 frames are being saved to U:\krios\20190717\PilA_3720.tif 27 frames are being saved to U:\krios\20190717\PilA_3721.tif Running script 17 at item 692 (-727.64, -47.05) Remaining time to fill dewars is 3720 Dewars are NOT busy filling Trimming A by 21 120 140 20, reference by 235 20 20 230 Trimming A by 21 120 140 20, reference by 240 24 165 20 Trimming A by 21 120 140 20, reference by 20 140 20 165 Trimming A by 21 120 140 20, reference by 23 125 125 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.17 Trimming A by 20 20 20 20, reference by 23 125 125 20 Disparity in stage position after aligning to target position: -0.60 -0.35 (2nd move error -0.23 -0.21) Finished aligning to item in 2 rounds -17.3 42.2 unbinned pixels; 177.7 1.1 nm along two shift axes; -160.4 -76.3 nm on specimen axes Total_Shift = 91.20167 6.3 29.3 unbinned pixels; 99.5 66.7 nm along two shift axes; -115.3 18.2 nm on specimen axes Total_Shift = 59.900698 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.52 microns changed by -2.62 to target drift = 0.02 nm/sec Measured defocus = -2.16 microns changed by 0.06 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3722.tif 27 frames are being saved to U:\krios\20190717\PilA_3723.tif 27 frames are being saved to U:\krios\20190717\PilA_3724.tif 27 frames are being saved to U:\krios\20190717\PilA_3725.tif 27 frames are being saved to U:\krios\20190717\PilA_3726.tif 27 frames are being saved to U:\krios\20190717\PilA_3727.tif Running script 17 at item 693 (-725.05, -48.81) Remaining time to fill dewars is 3540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 35 35 20, reference by 23 125 125 20 Disparity in stage position after aligning to target position: -0.42 -0.35 (2nd move error -0.05 -0.21) Finished aligning to item in 1 rounds Trimming A by 20 40 35 20, reference by 21 35 35 20 -25.3 10.1 unbinned pixels; 70.4 75.9 nm along two shift axes; -34.1 -100.7 nm on specimen axes Total_Shift = 54.578432 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.55 microns changed by -3.75 to target drift = 0.03 nm/sec Measured defocus = -2.23 microns changed by 0.03 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3728.tif 27 frames are being saved to U:\krios\20190717\PilA_3729.tif 27 frames are being saved to U:\krios\20190717\PilA_3730.tif 27 frames are being saved to U:\krios\20190717\PilA_3731.tif 27 frames are being saved to U:\krios\20190717\PilA_3732.tif 27 frames are being saved to U:\krios\20190717\PilA_3733.tif Running script 17 at item 694 (-722.54, -50.56) Remaining time to fill dewars is 3420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 110 95 20, reference by 23 125 125 20 Disparity in stage position after aligning to target position: -0.34 -0.25 (2nd move error 0.03 -0.11) Finished aligning to item in 1 rounds Trimming A by 22 110 95 20, reference by 22 110 95 20 -14.9 -1.8 unbinned pixels; 12.7 56.3 nm along two shift axes; 10.3 -57.5 nm on specimen axes Total_Shift = 29.987458 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.04 microns changed by -2.34 to target drift = 0.12 nm/sec Measured defocus = -2.34 microns changed by 0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3734.tif 27 frames are being saved to U:\krios\20190717\PilA_3735.tif 27 frames are being saved to U:\krios\20190717\PilA_3736.tif 27 frames are being saved to U:\krios\20190717\PilA_3737.tif 27 frames are being saved to U:\krios\20190717\PilA_3738.tif 27 frames are being saved to U:\krios\20190717\PilA_3739.tif Running script 17 at item 695 (-724.16, -53.11) Remaining time to fill dewars is 3240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 130 85 20, reference by 23 125 125 20 Disparity in stage position after aligning to target position: -0.34 -0.37 (2nd move error 0.02 -0.23) Finished aligning to item in 1 rounds Trimming A by 24 135 85 20, reference by 20 130 85 20 -27.5 -0.4 unbinned pixels; 34.4 99.5 nm along two shift axes; 7.3 -106.8 nm on specimen axes Total_Shift = 54.975263 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.34 microns changed by -3.74 to target drift = 0.08 nm/sec Measured defocus = -2.52 microns changed by 0.12 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3740.tif 27 frames are being saved to U:\krios\20190717\PilA_3741.tif 27 frames are being saved to U:\krios\20190717\PilA_3742.tif 27 frames are being saved to U:\krios\20190717\PilA_3743.tif 27 frames are being saved to U:\krios\20190717\PilA_3744.tif 27 frames are being saved to U:\krios\20190717\PilA_3745.tif Running script 17 at item 696 (-726.68, -51.42) Remaining time to fill dewars is 3060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 85 20 70, reference by 23 125 125 20 Disparity in stage position after aligning to target position: -0.46 -0.45 (2nd move error -0.09 -0.30) Finished aligning to item in 1 rounds Trimming A by 24 90 20 70, reference by 24 85 20 70 -21.4 20.4 unbinned pixels; 103.0 46.2 nm along two shift axes; -75.0 -87.5 nm on specimen axes Total_Shift = 59.1352 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.02 microns changed by -2.48 to target drift = 0.01 nm/sec Measured defocus = -2.53 microns changed by 0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3746.tif 27 frames are being saved to U:\krios\20190717\PilA_3747.tif 27 frames are being saved to U:\krios\20190717\PilA_3748.tif 27 frames are being saved to U:\krios\20190717\PilA_3749.tif 27 frames are being saved to U:\krios\20190717\PilA_3750.tif 27 frames are being saved to U:\krios\20190717\PilA_3751.tif Running script 17 at item 697 (-729.31, -50.20) Remaining time to fill dewars is 2940 Dewars are NOT busy filling Trimming A by 24 135 20 160, reference by 235 20 20 230 Trimming A by 24 135 20 160, reference by 240 24 165 20 Trimming A by 24 135 20 160, reference by 20 140 20 165 Trimming A by 24 135 20 160, reference by 23 125 125 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.18 Trimming A by 21 85 55 80, reference by 20 140 20 165 Disparity in stage position after aligning to target position: -0.22 0.06 (2nd move error 0.11 0.22) Finished aligning to item in 2 rounds Trimming A by 20 75 20 80, reference by 21 85 55 80 -2.4 1.3 unbinned pixels; 7.7 6.7 nm along two shift axes; -4.4 -9.5 nm on specimen axes Total_Shift = 5.389439 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.04 microns changed by -2.56 to target drift = 0.11 nm/sec Measured defocus = -2.61 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3752.tif 27 frames are being saved to U:\krios\20190717\PilA_3753.tif 27 frames are being saved to U:\krios\20190717\PilA_3754.tif 27 frames are being saved to U:\krios\20190717\PilA_3755.tif 27 frames are being saved to U:\krios\20190717\PilA_3756.tif 27 frames are being saved to U:\krios\20190717\PilA_3757.tif Running script 17 at item 698 (-731.82, -48.53) Remaining time to fill dewars is 2700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 70 20 95, reference by 20 140 20 165 Disparity in stage position after aligning to target position: -0.35 -0.13 (2nd move error -0.01 0.02) Finished aligning to item in 1 rounds Trimming A by 20 70 20 95, reference by 20 70 20 95 -0.7 1.3 unbinned pixels; 5.8 0.7 nm along two shift axes; -4.9 -3.2 nm on specimen axes Total_Shift = 3.012652 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.79 microns changed by -2.69 to target drift = 0.14 nm/sec Measured defocus = -0.86 microns changed by -0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3758.tif 27 frames are being saved to U:\krios\20190717\PilA_3759.tif 27 frames are being saved to U:\krios\20190717\PilA_3760.tif 27 frames are being saved to U:\krios\20190717\PilA_3761.tif 27 frames are being saved to U:\krios\20190717\PilA_3762.tif 27 frames are being saved to U:\krios\20190717\PilA_3763.tif Running script 17 at item 699 (-734.36, -46.79) Remaining time to fill dewars is 2640 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 54 21 20 170, reference by 20 140 20 165 Disparity in stage position after aligning to target position: -0.36 -0.22 (2nd move error -0.03 -0.07) Finished aligning to item in 1 rounds Trimming A by 46 23 20 175, reference by 54 21 20 170 -8.2 7.3 unbinned pixels; 37.4 18.7 nm along two shift axes; -26.5 -33.6 nm on specimen axes Total_Shift = 21.946713 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 3.09 microns changed by -4.09 to target drift = 0.12 nm/sec Measured defocus = -1.07 microns changed by 0.07 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3764.tif 27 frames are being saved to U:\krios\20190717\PilA_3765.tif 27 frames are being saved to U:\krios\20190717\PilA_3766.tif 27 frames are being saved to U:\krios\20190717\PilA_3767.tif 27 frames are being saved to U:\krios\20190717\PilA_3768.tif 27 frames are being saved to U:\krios\20190717\PilA_3769.tif Running script 17 at item 700 (-733.41, -51.13) Remaining time to fill dewars is 2340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 135 20 165, reference by 20 140 20 165 Disparity in stage position after aligning to target position: -0.27 -0.17 (2nd move error 0.07 -0.01) Finished aligning to item in 1 rounds Trimming A by 20 135 20 165, reference by 20 135 20 165 -3.1 -1.2 unbinned pixels; 0.5 12.9 nm along two shift axes; 5.4 -11.7 nm on specimen axes Total_Shift = 6.613418 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.68 microns changed by -2.78 to target drift = 0.09 nm/sec Measured defocus = -1.16 microns changed by 0.07 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3770.tif 27 frames are being saved to U:\krios\20190717\PilA_3771.tif 27 frames are being saved to U:\krios\20190717\PilA_3772.tif 27 frames are being saved to U:\krios\20190717\PilA_3773.tif 27 frames are being saved to U:\krios\20190717\PilA_3774.tif 27 frames are being saved to U:\krios\20190717\PilA_3775.tif Running script 17 at item 701 (-730.97, -52.82) Remaining time to fill dewars is 2160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 160 20 145, reference by 20 140 20 165 Disparity in stage position after aligning to target position: -0.18 -0.01 (2nd move error 0.16 0.14) Finished aligning to item in 1 rounds Trimming A by 20 155 20 140, reference by 20 160 20 145 3.3 0.0 unbinned pixels; 4.3 12.0 nm along two shift axes; -0.8 13.0 nm on specimen axes Total_Shift = 6.681023 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.82 microns changed by -4.02 to target drift = 0.16 nm/sec Measured defocus = -1.29 microns changed by 0.09 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3776.tif 27 frames are being saved to U:\krios\20190717\PilA_3777.tif 27 frames are being saved to U:\krios\20190717\PilA_3778.tif 27 frames are being saved to U:\krios\20190717\PilA_3779.tif 27 frames are being saved to U:\krios\20190717\PilA_3780.tif 27 frames are being saved to U:\krios\20190717\PilA_3781.tif Running script 17 at item 702 (-728.40, -54.39) Remaining time to fill dewars is 1980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 175 20 130, reference by 20 140 20 165 Disparity in stage position after aligning to target position: -0.13 0.08 (2nd move error 0.21 0.23) Finished aligning to item in 1 rounds Trimming A by 20 170 20 125, reference by 20 175 20 130 2.6 -6.0 unbinned pixels; 25.3 0.4 nm along two shift axes; 22.6 11.4 nm on specimen axes Total_Shift = 13.014485 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.23 microns changed by -2.53 to target drift = 0.17 nm/sec Measured defocus = -1.27 microns changed by -0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3782.tif 27 frames are being saved to U:\krios\20190717\PilA_3783.tif 27 frames are being saved to U:\krios\20190717\PilA_3784.tif 27 frames are being saved to U:\krios\20190717\PilA_3785.tif 27 frames are being saved to U:\krios\20190717\PilA_3786.tif 27 frames are being saved to U:\krios\20190717\PilA_3787.tif Running script 17 at item 703 (-444.74, -53.84) Remaining time to fill dewars is 1800 Dewars are NOT busy filling Trimming A by 226 24 170 20, reference by 226 21 20 255 Trimming A by 226 24 170 20, reference by 215 22 160 20 Trimming A by 226 24 170 20, reference by 22 190 20 225 Trimming A by 226 24 170 20, reference by 20 175 155 20 Disparity in stage position after aligning to center of map frame: -0.50 -0.22 Trimming A by 105 21 150 20, reference by 215 22 160 20 Disparity in stage position after aligning to target position: -0.40 -0.11 (2nd move error 0.10 0.08) Finished aligning to item in 2 rounds Trimming A by 110 21 150 20, reference by 105 21 150 20 -2.8 -1.4 unbinned pixels; 1.6 12.2 nm along two shift axes; 6.2 -10.5 nm on specimen axes Total_Shift = 6.263754 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.09 microns changed by -3.49 to target drift = 0.28 nm/sec Measured defocus = -1.34 microns changed by -0.06 to target drift = 0.18 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3788.tif 27 frames are being saved to U:\krios\20190717\PilA_3789.tif 27 frames are being saved to U:\krios\20190717\PilA_3790.tif 27 frames are being saved to U:\krios\20190717\PilA_3791.tif 27 frames are being saved to U:\krios\20190717\PilA_3792.tif 27 frames are being saved to U:\krios\20190717\PilA_3793.tif Running script 17 at item 704 (-447.22, -52.22) Remaining time to fill dewars is 1680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 20 120 20, reference by 215 22 160 20 Disparity in stage position after aligning to target position: -0.39 -0.28 (2nd move error 0.10 -0.07) Finished aligning to item in 1 rounds Trimming A by 125 21 115 20, reference by 115 20 120 20 -7.6 -0.7 unbinned pixels; 7.5 28.3 nm along two shift axes; 4.2 -29.4 nm on specimen axes Total_Shift = 15.243361 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.77 microns changed by -2.27 to target drift = 0.07 nm/sec Measured defocus = -1.54 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3794.tif 27 frames are being saved to U:\krios\20190717\PilA_3795.tif 27 frames are being saved to U:\krios\20190717\PilA_3796.tif 27 frames are being saved to U:\krios\20190717\PilA_3797.tif 27 frames are being saved to U:\krios\20190717\PilA_3798.tif 27 frames are being saved to U:\krios\20190717\PilA_3799.tif Running script 17 at item 705 (-449.67, -50.49) Remaining time to fill dewars is 1500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 23 95 20, reference by 215 22 160 20 Disparity in stage position after aligning to target position: -0.39 -0.34 (2nd move error 0.11 -0.13) Finished aligning to item in 1 rounds Trimming A by 140 23 95 20, reference by 140 23 95 20 -14.8 -0.2 unbinned pixels; 18.7 53.5 nm along two shift axes; 3.8 -57.5 nm on specimen axes Total_Shift = 29.598664 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.12 microns changed by -3.72 to target drift = 0.05 nm/sec Measured defocus = -1.65 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3800.tif 27 frames are being saved to U:\krios\20190717\PilA_3801.tif 27 frames are being saved to U:\krios\20190717\PilA_3802.tif 27 frames are being saved to U:\krios\20190717\PilA_3803.tif 27 frames are being saved to U:\krios\20190717\PilA_3804.tif 27 frames are being saved to U:\krios\20190717\PilA_3805.tif Running script 17 at item 706 (-452.22, -48.79) Remaining time to fill dewars is 1320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 180 20 45 90, reference by 215 22 160 20 Disparity in stage position after aligning to target position: -0.46 -0.43 (2nd move error 0.04 -0.23) Finished aligning to item in 1 rounds Trimming A by 180 20 40 85, reference by 180 20 45 90 -27.0 -2.0 unbinned pixels; 27.7 100.1 nm along two shift axes; 13.6 -104.4 nm on specimen axes Total_Shift = 54.057322 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.73 microns changed by -2.43 to target drift = 0.11 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3806.tif 27 frames are being saved to U:\krios\20190717\PilA_3807.tif 27 frames are being saved to U:\krios\20190717\PilA_3808.tif 27 frames are being saved to U:\krios\20190717\PilA_3809.tif 27 frames are being saved to U:\krios\20190717\PilA_3810.tif 27 frames are being saved to U:\krios\20190717\PilA_3811.tif Running script 17 at item 707 (-454.02, -51.87) Remaining time to fill dewars is 1140 Dewars are NOT busy filling Trimming A by 235 23 20 245, reference by 226 21 20 255 Trimming A by 235 23 20 245, reference by 215 22 160 20 Trimming A by 235 23 20 245, reference by 22 190 20 225 Trimming A by 235 23 20 245, reference by 20 175 155 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.14 Trimming A by 125 22 20 110, reference by 226 21 20 255 Disparity in stage position after aligning to target position: -0.10 0.08 (2nd move error 0.24 0.20) Finished aligning to item in 2 rounds Trimming A by 130 22 20 105, reference by 125 22 20 110 0.1 2.8 unbinned pixels; 10.4 4.6 nm along two shift axes; -11.1 -0.3 nm on specimen axes Total_Shift = 5.700812 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.91 microns changed by -3.71 to target drift = 0.11 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3812.tif 27 frames are being saved to U:\krios\20190717\PilA_3813.tif 27 frames are being saved to U:\krios\20190717\PilA_3814.tif 27 frames are being saved to U:\krios\20190717\PilA_3815.tif 27 frames are being saved to U:\krios\20190717\PilA_3816.tif 27 frames are being saved to U:\krios\20190717\PilA_3817.tif Running script 17 at item 708 (-451.29, -53.02) Remaining time to fill dewars is 900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 21 30 50, reference by 215 22 160 20 Disparity in stage position after aligning to target position: -0.35 -0.38 (2nd move error 0.14 -0.18) Finished aligning to item in 1 rounds Trimming A by 60 20 40 45, reference by 65 21 30 50 -20.7 -0.8 unbinned pixels; 24.2 75.6 nm along two shift axes; 7.3 -80.4 nm on specimen axes Total_Shift = 41.418684 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.42 microns changed by -2.32 to target drift = 0.13 nm/sec Measured defocus = -1.90 microns changed by -0.00 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3818.tif 27 frames are being saved to U:\krios\20190717\PilA_3819.tif 27 frames are being saved to U:\krios\20190717\PilA_3820.tif 27 frames are being saved to U:\krios\20190717\PilA_3821.tif 27 frames are being saved to U:\krios\20190717\PilA_3822.tif 27 frames are being saved to U:\krios\20190717\PilA_3823.tif Running script 17 at item 709 (-448.79, -54.73) Remaining time to fill dewars is 780 Dewars are NOT busy filling Trimming A by 225 22 165 20, reference by 226 21 20 255 Trimming A by 225 22 165 20, reference by 215 22 160 20 Trimming A by 225 22 165 20, reference by 22 190 20 225 Trimming A by 225 22 165 20, reference by 20 175 155 20 Disparity in stage position after aligning to center of map frame: -0.36 -0.18 Trimming A by 55 20 50 20, reference by 215 22 160 20 Disparity in stage position after aligning to target position: -0.30 -0.27 (2nd move error 0.04 -0.11) Finished aligning to item in 2 rounds Trimming A by 50 20 55 20, reference by 55 20 50 20 -13.6 -1.5 unbinned pixels; 12.0 51.2 nm along two shift axes; 8.9 -52.5 nm on specimen axes Total_Shift = 27.322572 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.53 microns changed by -3.53 to target drift = 0.08 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3824.tif 27 frames are being saved to U:\krios\20190717\PilA_3825.tif 27 frames are being saved to U:\krios\20190717\PilA_3826.tif 27 frames are being saved to U:\krios\20190717\PilA_3827.tif 27 frames are being saved to U:\krios\20190717\PilA_3828.tif 27 frames are being saved to U:\krios\20190717\PilA_3829.tif Running script 17 at item 710 (-446.13, -56.40) Remaining time to fill dewars is 600 Dewars are NOT busy filling Trimming A by 23 180 160 20, reference by 226 21 20 255 Trimming A by 23 180 160 20, reference by 215 22 160 20 Trimming A by 23 180 160 20, reference by 22 190 20 225 Trimming A by 23 180 160 20, reference by 20 175 155 20 Disparity in stage position after aligning to center of map frame: -0.34 -0.26 Trimming A by 22 56 115 20, reference by 20 175 155 20 Disparity in stage position after aligning to target position: -0.39 -0.30 (2nd move error -0.06 -0.07) Finished aligning to item in 2 rounds Trimming A by 22 56 115 20, reference by 22 56 115 20 -10.2 15.2 unbinned pixels; 69.1 14.1 nm along two shift axes; -56.8 -43.1 nm on specimen axes Total_Shift = 36.581061 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.01 microns changed by -2.10 to target drift = 0.06 nm/sec Measured defocus = -2.04 microns changed by -0.06 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3830.tif 27 frames are being saved to U:\krios\20190717\PilA_3831.tif 27 frames are being saved to U:\krios\20190717\PilA_3832.tif 27 frames are being saved to U:\krios\20190717\PilA_3833.tif 27 frames are being saved to U:\krios\20190717\PilA_3834.tif 27 frames are being saved to U:\krios\20190717\PilA_3835.tif Running script 17 at item 711 (-445.25, -60.75) Remaining time to fill dewars is 540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 147 170 20, reference by 20 175 155 20 Disparity in stage position after aligning to target position: -0.32 -0.20 (2nd move error 0.01 0.04) Finished aligning to item in 1 rounds Trimming A by 20 147 170 20, reference by 20 147 170 20 -1.2 -0.3 unbinned pixels; 0.4 4.8 nm along two shift axes; 1.5 -4.7 nm on specimen axes Total_Shift = 2.50811 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.25 microns changed by -3.45 to target drift = 0.12 nm/sec Measured defocus = -2.26 microns changed by 0.06 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3836.tif 27 frames are being saved to U:\krios\20190717\PilA_3837.tif 27 frames are being saved to U:\krios\20190717\PilA_3838.tif 27 frames are being saved to U:\krios\20190717\PilA_3839.tif 27 frames are being saved to U:\krios\20190717\PilA_3840.tif 27 frames are being saved to U:\krios\20190717\PilA_3841.tif Running script 17 at item 712 (-447.79, -59.00) Remaining time to fill dewars is 300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 81 95 20, reference by 20 175 155 20 Disparity in stage position after aligning to target position: -0.35 -0.33 (2nd move error -0.02 -0.09) Finished aligning to item in 1 rounds Trimming A by 20 76 100 20, reference by 23 81 95 20 -11.3 5.3 unbinned pixels; 34.2 32.8 nm along two shift axes; -18.2 -45.1 nm on specimen axes Total_Shift = 24.981402 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.16 microns changed by -2.14 to target drift = 0.03 nm/sec Measured defocus = -2.30 microns changed by 0.00 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3842.tif 27 frames are being saved to U:\krios\20190717\PilA_3843.tif 27 frames are being saved to U:\krios\20190717\PilA_3844.tif 27 frames are being saved to U:\krios\20190717\PilA_3845.tif 27 frames are being saved to U:\krios\20190717\PilA_3846.tif 27 frames are being saved to U:\krios\20190717\PilA_3847.tif Running script 17 at item 713 (-450.37, -57.26) Remaining time to fill dewars is 60 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 175 155 20 Disparity in stage position after aligning to target position: -0.39 -0.41 (2nd move error -0.06 -0.17) Finished aligning to item in 1 rounds -21.6 11.6 unbinned pixels; 70.8 60.2 nm along two shift axes; -40.6 -86.4 nm on specimen axes Total_Shift = 48.983222 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 3.60 microns changed by -6.00 to target drift = 1.18 nm/sec Measured defocus = -5.07 microns changed by 2.67 to target drift = 0.04 nm/sec Measured defocus = -3.43 microns changed by 1.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3848.tif 27 frames are being saved to U:\krios\20190717\PilA_3849.tif 27 frames are being saved to U:\krios\20190717\PilA_3850.tif 27 frames are being saved to U:\krios\20190717\PilA_3851.tif 27 frames are being saved to U:\krios\20190717\PilA_3852.tif 27 frames are being saved to U:\krios\20190717\PilA_3853.tif Running script 17 at item 714 (-452.95, -55.55) Remaining time to fill dewars is 3780 Dewars ARE busy filling Dewars ARE busy filling Dewars are NOT busy filling Trimming A by 235 23 20 245, reference by 226 21 20 255 Trimming A by 235 23 20 245, reference by 215 22 160 20 Trimming A by 235 23 20 245, reference by 22 190 20 225 Trimming A by 235 23 20 245, reference by 20 175 155 20 Disparity in stage position after aligning to center of map frame: -0.30 -0.15 Trimming A by 35 21 20 60, reference by 226 21 20 255 Disparity in stage position after aligning to target position: -0.02 0.14 (2nd move error 0.27 0.26) Finished aligning to item in 2 rounds Trimming A by 45 21 20 50, reference by 35 21 20 60 1.7 0.8 unbinned pixels; 0.8 7.2 nm along two shift axes; -3.5 6.3 nm on specimen axes Total_Shift = 3.707346 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.22 microns changed by -2.28 to target drift = 0.16 nm/sec Measured defocus = -2.48 microns changed by -0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3854.tif 27 frames are being saved to U:\krios\20190717\PilA_3855.tif 27 frames are being saved to U:\krios\20190717\PilA_3856.tif 27 frames are being saved to U:\krios\20190717\PilA_3857.tif 27 frames are being saved to U:\krios\20190717\PilA_3858.tif 27 frames are being saved to U:\krios\20190717\PilA_3859.tif Running script 17 at item 715 (-455.69, -54.38) Remaining time to fill dewars is 31440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 73 20 20 145, reference by 226 21 20 255 Disparity in stage position after aligning to target position: -0.14 -0.02 (2nd move error 0.15 0.10) Finished aligning to item in 1 rounds Trimming A by 91 24 20 130, reference by 73 20 20 145 -0.3 -1.6 unbinned pixels; 5.4 3.6 nm along two shift axes; 6.3 -1.0 nm on specimen axes Total_Shift = 3.270389 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 1.07 microns changed by -3.67 to target drift = 0.15 nm/sec Measured defocus = -2.63 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3860.tif 27 frames are being saved to U:\krios\20190717\PilA_3861.tif 27 frames are being saved to U:\krios\20190717\PilA_3862.tif 27 frames are being saved to U:\krios\20190717\PilA_3863.tif 27 frames are being saved to U:\krios\20190717\PilA_3864.tif 27 frames are being saved to U:\krios\20190717\PilA_3865.tif Running script 17 at item 716 (-457.07, -56.97) Remaining time to fill dewars is 31260 Dewars are NOT busy filling Trimming A by 20 190 20 215, reference by 226 21 20 255 Trimming A by 20 190 20 215, reference by 215 22 160 20 Trimming A by 20 190 20 215, reference by 22 190 20 225 Trimming A by 20 190 20 215, reference by 20 175 155 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.20 Trimming A by 61 30 20 145, reference by 22 190 20 225 Disparity in stage position after aligning to target position: -0.22 -0.12 (2nd move error -0.00 0.05) Finished aligning to item in 2 rounds Trimming A by 57 28 20 150, reference by 61 30 20 145 -2.7 1.3 unbinned pixels; 8.2 7.7 nm along two shift axes; -4.4 -10.7 nm on specimen axes Total_Shift = 5.939612 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.59 microns changed by -2.49 to target drift = 0.09 nm/sec Measured defocus = -0.88 microns changed by -0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3866.tif 27 frames are being saved to U:\krios\20190717\PilA_3867.tif 27 frames are being saved to U:\krios\20190717\PilA_3868.tif 27 frames are being saved to U:\krios\20190717\PilA_3869.tif 27 frames are being saved to U:\krios\20190717\PilA_3870.tif 27 frames are being saved to U:\krios\20190717\PilA_3871.tif Running script 17 at item 717 (-454.53, -58.68) Remaining time to fill dewars is 31140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 70 20 95, reference by 22 190 20 225 Disparity in stage position after aligning to target position: -0.12 -0.05 (2nd move error 0.09 0.12) Finished aligning to item in 1 rounds Trimming A by 21 70 20 90, reference by 21 70 20 95 -0.3 0.8 unbinned pixels; 3.2 0.1 nm along two shift axes; -2.9 -1.3 nm on specimen axes Total_Shift = 1.639192 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.69 microns changed by -3.69 to target drift = 0.17 nm/sec Measured defocus = -1.04 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3872.tif 27 frames are being saved to U:\krios\20190717\PilA_3873.tif 27 frames are being saved to U:\krios\20190717\PilA_3874.tif 27 frames are being saved to U:\krios\20190717\PilA_3875.tif 27 frames are being saved to U:\krios\20190717\PilA_3876.tif 27 frames are being saved to U:\krios\20190717\PilA_3877.tif Running script 17 at item 718 (-451.96, -59.92) Remaining time to fill dewars is 31020 Dewars are NOT busy filling Trimming A by 23 180 160 20, reference by 226 21 20 255 Trimming A by 23 180 160 20, reference by 215 22 160 20 Trimming A by 23 180 160 20, reference by 22 190 20 225 Trimming A by 23 180 160 20, reference by 20 175 155 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.18 Trimming A by 24 80 20 70, reference by 20 175 155 20 Disparity in stage position after aligning to target position: -0.37 -0.47 (2nd move error -0.08 -0.32) Finished aligning to item in 2 rounds Trimming A by 24 85 20 70, reference by 24 80 20 70 -24.8 15.9 unbinned pixels; 90.7 65.4 nm along two shift axes; -56.5 -99.9 nm on specimen axes Total_Shift = 58.901789 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.62 microns changed by -3.72 to target drift = 0.04 nm/sec Measured defocus = -1.20 microns changed by 0.10 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3878.tif 27 frames are being saved to U:\krios\20190717\PilA_3879.tif 27 frames are being saved to U:\krios\20190717\PilA_3880.tif 27 frames are being saved to U:\krios\20190717\PilA_3881.tif 27 frames are being saved to U:\krios\20190717\PilA_3882.tif 27 frames are being saved to U:\krios\20190717\PilA_3883.tif Running script 17 at item 719 (-456.12, -61.24) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 140 20 155, reference by 22 190 20 225 Disparity in stage position after aligning to target position: -0.24 -0.13 (2nd move error -0.02 0.04) Finished aligning to item in 1 rounds Trimming A by 24 140 20 155, reference by 24 140 20 155 -0.9 5.2 unbinned pixels; 20.5 4.5 nm along two shift axes; -20.2 -4.8 nm on specimen axes Total_Shift = 10.649697 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.45 microns changed by -3.65 to target drift = 0.14 nm/sec Measured defocus = -1.01 microns changed by -0.19 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3884.tif 27 frames are being saved to U:\krios\20190717\PilA_3885.tif 27 frames are being saved to U:\krios\20190717\PilA_3886.tif 27 frames are being saved to U:\krios\20190717\PilA_3887.tif 27 frames are being saved to U:\krios\20190717\PilA_3888.tif 27 frames are being saved to U:\krios\20190717\PilA_3889.tif Running script 17 at item 742-1 (-9.20, -159.23) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Trimming A by 24 71 185 20, reference by 308 22 20 235 Trimming A by 24 71 185 20, reference by 20 20 20 20 Trimming A by 24 71 185 20, reference by 21 75 20 125 Trimming A by 24 71 185 20, reference by 23 95 165 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.10 Trimming A by 20 44 160 20, reference by 23 95 165 20 Disparity in stage position after aligning to target position: -0.10 -0.11 (2nd move error -0.00 -0.04) Finished aligning to item in 2 rounds Trimming A by 22 46 160 20, reference by 20 44 160 20 -6.5 2.4 unbinned pixels; 17.1 19.7 nm along two shift axes; -7.8 -25.7 nm on specimen axes Total_Shift = 13.7805 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = -0.17 microns changed by -1.13 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3890.tif 27 frames are being saved to U:\krios\20190717\PilA_3891.tif 27 frames are being saved to U:\krios\20190717\PilA_3892.tif 27 frames are being saved to U:\krios\20190717\PilA_3893.tif 27 frames are being saved to U:\krios\20190717\PilA_3894.tif 27 frames are being saved to U:\krios\20190717\PilA_3895.tif Running script 17 at item 743-1 (-10.09, -154.84) Remaining time to fill dewars is 30720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 20 135 20, reference by 23 95 165 20 Disparity in stage position after aligning to target position: -0.19 -0.17 (2nd move error -0.09 -0.10) Finished aligning to item in 1 rounds Trimming A by 115 24 130 20, reference by 115 20 135 20 -11.5 16.4 unbinned pixels; 75.4 16.7 nm along two shift axes; -61.4 -48.2 nm on specimen axes Total_Shift = 40.088205 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.09 microns changed by -2.49 to target drift = 0.07 nm/sec Measured defocus = -1.44 microns changed by 0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3896.tif 27 frames are being saved to U:\krios\20190717\PilA_3897.tif 27 frames are being saved to U:\krios\20190717\PilA_3898.tif 27 frames are being saved to U:\krios\20190717\PilA_3899.tif 27 frames are being saved to U:\krios\20190717\PilA_3900.tif 27 frames are being saved to U:\krios\20190717\PilA_3901.tif Running script 17 at item 744-1 (-11.76, -157.49) Remaining time to fill dewars is 30660 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 40 21 60 20, reference by 23 95 165 20 Disparity in stage position after aligning to target position: -0.14 -0.22 (2nd move error -0.03 -0.15) Finished aligning to item in 1 rounds Trimming A by 45 31 55 20, reference by 40 21 60 20 -19.9 8.7 unbinned pixels; 58.0 58.7 nm along two shift axes; -29.6 -79.4 nm on specimen axes Total_Shift = 43.525132 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.94 microns changed by -2.44 to target drift = 0.02 nm/sec Measured defocus = -1.50 microns changed by -0.00 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3902.tif 27 frames are being saved to U:\krios\20190717\PilA_3903.tif 27 frames are being saved to U:\krios\20190717\PilA_3904.tif 27 frames are being saved to U:\krios\20190717\PilA_3905.tif 27 frames are being saved to U:\krios\20190717\PilA_3906.tif 27 frames are being saved to U:\krios\20190717\PilA_3907.tif Running script 17 at item 745-1 (-12.73, -153.15) Remaining time to fill dewars is 30540 Dewars are NOT busy filling Trimming A by 324 20 255 20, reference by 308 22 20 235 Trimming A by 324 20 255 20, reference by 20 20 20 20 Trimming A by 324 20 255 20, reference by 21 75 20 125 Trimming A by 324 20 255 20, reference by 23 95 165 20 Disparity in stage position after aligning to center of map frame: -0.37 -0.85 Trimming A by 96 23 105 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -1.60 -2.34 (2nd move error -1.24 -1.51) Finished aligning to item in 2 rounds Trimming A by 70 20 65 20, reference by 96 23 105 20 -8.1 200.2 unbinned pixels; 746.6 272.1 nm along two shift axes; -777.0 -73.7 nm on specimen axes Total_Shift = 400.647788 Trimming A by 60 20 55 20, reference by 96 23 105 20 25.0 63.1 unbinned pixels; 199.2 185.1 nm along two shift axes; -250.7 84.2 nm on specimen axes Total_Shift = 135.735297 Total_shift never < 120: Skiping ................................................ Running script 17 at item 746-1 (-14.19, -155.73) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 20 45 20, reference by 23 95 165 20 Disparity in stage position after aligning to target position: -0.19 -0.35 (2nd move error -0.09 -0.28) Finished aligning to item in 1 rounds Trimming A by 50 20 45 20, reference by 55 20 45 20 -29.5 19.7 unbinned pixels; 111.0 76.7 nm along two shift axes; -70.5 -119.1 nm on specimen axes Total_Shift = 71.019705 Trimming A by 50 20 40 20, reference by 55 20 45 20 -2.8 11.8 unbinned pixels; 46.8 7.8 nm along two shift axes; -45.1 -13.2 nm on specimen axes Total_Shift = 24.141648 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.91 microns changed by -2.51 to target drift = 0.01 nm/sec Measured defocus = -1.63 microns changed by 0.03 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3908.tif 27 frames are being saved to U:\krios\20190717\PilA_3909.tif 27 frames are being saved to U:\krios\20190717\PilA_3910.tif 27 frames are being saved to U:\krios\20190717\PilA_3911.tif 27 frames are being saved to U:\krios\20190717\PilA_3912.tif 27 frames are being saved to U:\krios\20190717\PilA_3913.tif Running script 17 at item 747-1 (-15.22, -151.46) Remaining time to fill dewars is 30300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 20 70 50, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 1.54 -2.09 (2nd move error 1.91 -1.26) Finished aligning to item in 1 rounds Trimming A by 115 22 85 20, reference by 130 20 70 50 -1.1 -19.4 unbinned pixels; 70.1 33.1 nm along two shift axes; 75.9 -0.0 nm on specimen axes Total_Shift = 38.947161 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.77 microns changed by -2.47 to target drift = 0.16 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3914.tif 27 frames are being saved to U:\krios\20190717\PilA_3915.tif 27 frames are being saved to U:\krios\20190717\PilA_3916.tif 27 frames are being saved to U:\krios\20190717\PilA_3917.tif 27 frames are being saved to U:\krios\20190717\PilA_3918.tif 27 frames are being saved to U:\krios\20190717\PilA_3919.tif Running script 17 at item 748 (-16.75, -154.68) Remaining time to fill dewars is 30240 Dewars are NOT busy filling Trimming A by 21 75 20 125, reference by 308 22 20 235 Trimming A by 21 75 20 125, reference by 20 20 20 20 Trimming A by 21 75 20 125, reference by 21 75 20 125 Trimming A by 21 75 20 125, reference by 23 95 165 20 Disparity in stage position after aligning to center of map frame: -0.10 -0.01 Trimming A by 80 20 20 70, reference by 21 75 20 125 Disparity in stage position after aligning to target position: -0.10 0.20 (2nd move error -0.01 0.19) Finished aligning to item in 2 rounds Trimming A by 85 24 20 70, reference by 80 20 20 70 -2.0 4.0 unbinned pixels; 17.3 1.4 nm along two shift axes; -15.1 -8.8 nm on specimen axes Total_Shift = 8.955474 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.68 microns changed by -2.48 to target drift = 0.13 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3920.tif 27 frames are being saved to U:\krios\20190717\PilA_3921.tif 27 frames are being saved to U:\krios\20190717\PilA_3922.tif 27 frames are being saved to U:\krios\20190717\PilA_3923.tif 27 frames are being saved to U:\krios\20190717\PilA_3924.tif 27 frames are being saved to U:\krios\20190717\PilA_3925.tif Running script 17 at item 749 (-19.36, -152.89) Remaining time to fill dewars is 30000 Dewars are NOT busy filling Trimming A by 308 22 20 235, reference by 308 22 20 235 Trimming A by 308 22 20 235, reference by 20 20 20 20 Trimming A by 308 22 20 235, reference by 21 75 20 125 Trimming A by 308 22 20 235, reference by 23 95 165 20 Disparity in stage position after aligning to center of map frame: -0.03 -0.02 Trimming A by 150 20 20 135, reference by 308 22 20 235 Disparity in stage position after aligning to target position: 0.10 0.07 (2nd move error 0.12 0.06) Finished aligning to item in 2 rounds Trimming A by 150 20 20 135, reference by 150 20 20 135 -1.8 -0.4 unbinned pixels; 0.9 6.9 nm along two shift axes; 1.8 -6.8 nm on specimen axes Total_Shift = 3.61953 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.58 microns changed by -2.48 to target drift = 0.13 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3926.tif 27 frames are being saved to U:\krios\20190717\PilA_3927.tif 27 frames are being saved to U:\krios\20190717\PilA_3928.tif 27 frames are being saved to U:\krios\20190717\PilA_3929.tif 27 frames are being saved to U:\krios\20190717\PilA_3930.tif 27 frames are being saved to U:\krios\20190717\PilA_3931.tif Running script 17 at item 750 (-20.87, -155.45) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 111 21 20 160, reference by 21 75 20 125 Disparity in stage position after aligning to target position: -0.14 0.02 (2nd move error -0.06 0.00) Finished aligning to item in 1 rounds Trimming A by 111 21 20 160, reference by 111 21 20 160 1.0 13.2 unbinned pixels; 47.4 23.3 nm along two shift axes; -51.7 0.9 nm on specimen axes Total_Shift = 26.529127 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.43 microns changed by -2.43 to target drift = 0.11 nm/sec Measured defocus = -2.03 microns changed by 0.03 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3932.tif 27 frames are being saved to U:\krios\20190717\PilA_3933.tif 27 frames are being saved to U:\krios\20190717\PilA_3934.tif 27 frames are being saved to U:\krios\20190717\PilA_3935.tif 27 frames are being saved to U:\krios\20190717\PilA_3936.tif 27 frames are being saved to U:\krios\20190717\PilA_3937.tif Running script 17 at item 751 (-18.34, -157.18) Remaining time to fill dewars is 29820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 46 20 20 85, reference by 21 75 20 125 Disparity in stage position after aligning to target position: -0.08 0.16 (2nd move error 0.01 0.14) Finished aligning to item in 1 rounds Trimming A by 55 22 20 80, reference by 46 20 20 85 -1.4 1.4 unbinned pixels; 6.9 3.1 nm along two shift axes; -5.0 -5.8 nm on specimen axes Total_Shift = 3.936265 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.30 microns changed by -2.40 to target drift = 0.15 nm/sec Measured defocus = -2.13 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3938.tif 27 frames are being saved to U:\krios\20190717\PilA_3939.tif 27 frames are being saved to U:\krios\20190717\PilA_3940.tif 27 frames are being saved to U:\krios\20190717\PilA_3941.tif 27 frames are being saved to U:\krios\20190717\PilA_3942.tif 27 frames are being saved to U:\krios\20190717\PilA_3943.tif Running script 17 at item 752 (-15.87, -158.95) Remaining time to fill dewars is 29580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 21 75 20 125 Disparity in stage position after aligning to target position: -0.05 0.23 (2nd move error 0.04 0.21) Finished aligning to item in 1 rounds -1.8 0.3 unbinned pixels; 3.3 6.0 nm along two shift axes; -0.7 -7.0 nm on specimen axes Total_Shift = 3.608304 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.13 microns changed by -2.33 to target drift = 0.17 nm/sec Measured defocus = -2.21 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3944.tif 27 frames are being saved to U:\krios\20190717\PilA_3945.tif 27 frames are being saved to U:\krios\20190717\PilA_3946.tif 27 frames are being saved to U:\krios\20190717\PilA_3947.tif 27 frames are being saved to U:\krios\20190717\PilA_3948.tif 27 frames are being saved to U:\krios\20190717\PilA_3949.tif Running script 17 at item 753 (-13.32, -160.04) Remaining time to fill dewars is 29520 Dewars are NOT busy filling Trimming A by 23 79 180 20, reference by 308 22 20 235 Trimming A by 23 79 180 20, reference by 20 20 20 20 Trimming A by 23 79 180 20, reference by 21 75 20 125 Trimming A by 23 79 180 20, reference by 23 95 165 20 Disparity in stage position after aligning to center of map frame: -0.09 0.01 Trimming A by 21 40 45 20, reference by 23 95 165 20 Disparity in stage position after aligning to target position: -0.11 -0.26 (2nd move error -0.03 -0.29) Finished aligning to item in 2 rounds Trimming A by 20 45 45 20, reference by 21 40 45 20 -27.5 7.1 unbinned pixels; 62.0 88.4 nm along two shift axes; -21.8 -108.5 nm on specimen axes Total_Shift = 56.829583 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.08 microns changed by -2.38 to target drift = 0.04 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3950.tif 27 frames are being saved to U:\krios\20190717\PilA_3951.tif 27 frames are being saved to U:\krios\20190717\PilA_3952.tif 27 frames are being saved to U:\krios\20190717\PilA_3953.tif 27 frames are being saved to U:\krios\20190717\PilA_3954.tif 27 frames are being saved to U:\krios\20190717\PilA_3955.tif Running script 17 at item 754 (-10.83, -161.81) Remaining time to fill dewars is 29400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 61 145 20, reference by 23 95 165 20 Disparity in stage position after aligning to target position: -0.02 -0.13 (2nd move error 0.05 -0.16) Finished aligning to item in 1 rounds Trimming A by 21 100 120 20, reference by 21 61 145 20 -18.4 -0.3 unbinned pixels; 22.7 66.6 nm along two shift axes; 5.2 -71.3 nm on specimen axes Total_Shift = 36.709654 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 12.49 microns changed by -14.89 to target drift = 6.86 nm/sec Measured defocus = -15.69 microns changed by 13.29 to target drift = 0.10 nm/sec Measured defocus = -9.10 microns changed by 6.70 to target drift = 0.06 nm/sec Measured defocus = -2.73 microns changed by 0.33 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3956.tif 27 frames are being saved to U:\krios\20190717\PilA_3957.tif 27 frames are being saved to U:\krios\20190717\PilA_3958.tif 27 frames are being saved to U:\krios\20190717\PilA_3959.tif 27 frames are being saved to U:\krios\20190717\PilA_3960.tif 27 frames are being saved to U:\krios\20190717\PilA_3961.tif Running script 17 at item 755 (-12.45, -164.34) Remaining time to fill dewars is 29280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 125 90 20, reference by 23 95 165 20 Disparity in stage position after aligning to target position: -0.02 -0.23 (2nd move error 0.06 -0.26) Finished aligning to item in 1 rounds Trimming A by 21 130 95 20, reference by 21 125 90 20 -28.0 -3.0 unbinned pixels; 25.6 105.2 nm along two shift axes; 17.4 -108.3 nm on specimen axes Total_Shift = 56.299178 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.15 microns changed by -2.35 to target drift = 0.18 nm/sec Measured defocus = -2.52 microns changed by 0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3962.tif 27 frames are being saved to U:\krios\20190717\PilA_3963.tif 27 frames are being saved to U:\krios\20190717\PilA_3964.tif 27 frames are being saved to U:\krios\20190717\PilA_3965.tif 27 frames are being saved to U:\krios\20190717\PilA_3966.tif 27 frames are being saved to U:\krios\20190717\PilA_3967.tif Running script 17 at item 756 (-14.77, -162.85) Remaining time to fill dewars is 29100 Dewars are NOT busy filling Trimming A by 21 75 20 125, reference by 308 22 20 235 Trimming A by 21 75 20 125, reference by 20 20 20 20 Trimming A by 21 75 20 125, reference by 21 75 20 125 Trimming A by 21 75 20 125, reference by 23 95 165 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.01 Trimming A by 20 75 25 60, reference by 21 75 20 125 Disparity in stage position after aligning to target position: 0.04 0.29 (2nd move error 0.15 0.27) Finished aligning to item in 2 rounds Trimming A by 21 65 35 55, reference by 20 75 25 60 3.8 4.3 unbinned pixels; 10.9 20.1 nm along two shift axes; -17.6 13.8 nm on specimen axes Total_Shift = 11.480779 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.30 microns changed by -2.30 to target drift = 0.13 nm/sec Measured defocus = -2.57 microns changed by -0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3968.tif 27 frames are being saved to U:\krios\20190717\PilA_3969.tif 27 frames are being saved to U:\krios\20190717\PilA_3970.tif 27 frames are being saved to U:\krios\20190717\PilA_3971.tif 27 frames are being saved to U:\krios\20190717\PilA_3972.tif 27 frames are being saved to U:\krios\20190717\PilA_3973.tif Running script 17 at item 757 (-17.49, -161.52) Remaining time to fill dewars is 28980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 65 20 80, reference by 21 75 20 125 Disparity in stage position after aligning to target position: -0.02 0.15 (2nd move error 0.09 0.14) Finished aligning to item in 1 rounds Trimming A by 20 65 20 75, reference by 21 65 20 80 0.6 0.5 unbinned pixels; 1.2 3.0 nm along two shift axes; -2.2 2.3 nm on specimen axes Total_Shift = 1.638674 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 0.19 microns changed by -1.09 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3974.tif 27 frames are being saved to U:\krios\20190717\PilA_3975.tif 27 frames are being saved to U:\krios\20190717\PilA_3976.tif 27 frames are being saved to U:\krios\20190717\PilA_3977.tif 27 frames are being saved to U:\krios\20190717\PilA_3978.tif 27 frames are being saved to U:\krios\20190717\PilA_3979.tif Running script 17 at item 758 (-19.98, -159.80) Remaining time to fill dewars is 28860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 42 20 110, reference by 21 75 20 125 Disparity in stage position after aligning to target position: -0.05 0.04 (2nd move error 0.06 0.03) Finished aligning to item in 1 rounds Trimming A by 31 55 20 95, reference by 21 42 20 110 -2.4 0.2 unbinned pixels; 3.8 8.5 nm along two shift axes; -0.2 -9.5 nm on specimen axes Total_Shift = 4.866436 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.40 microns changed by -2.40 to target drift = 0.14 nm/sec Measured defocus = -0.97 microns changed by -0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3980.tif 27 frames are being saved to U:\krios\20190717\PilA_3981.tif 27 frames are being saved to U:\krios\20190717\PilA_3982.tif 27 frames are being saved to U:\krios\20190717\PilA_3983.tif 27 frames are being saved to U:\krios\20190717\PilA_3984.tif 27 frames are being saved to U:\krios\20190717\PilA_3985.tif Running script 17 at item 759 (-19.09, -164.05) Remaining time to fill dewars is 28680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 140 20 135, reference by 21 75 20 125 Disparity in stage position after aligning to target position: -0.01 0.07 (2nd move error 0.09 0.05) Finished aligning to item in 1 rounds Trimming A by 21 140 65 130, reference by 21 140 20 135 -0.6 0.1 unbinned pixels; 1.2 2.1 nm along two shift axes; -0.3 -2.4 nm on specimen axes Total_Shift = 1.264702 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.27 microns changed by -2.37 to target drift = 0.14 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3986.tif 27 frames are being saved to U:\krios\20190717\PilA_3987.tif 27 frames are being saved to U:\krios\20190717\PilA_3988.tif 27 frames are being saved to U:\krios\20190717\PilA_3989.tif 27 frames are being saved to U:\krios\20190717\PilA_3990.tif 27 frames are being saved to U:\krios\20190717\PilA_3991.tif Running script 17 at item 760 (-16.58, -165.71) Remaining time to fill dewars is 28440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 160 20 120, reference by 21 75 20 125 Disparity in stage position after aligning to target position: 0.09 0.22 (2nd move error 0.19 0.21) Finished aligning to item in 1 rounds Trimming A by 21 155 75 110, reference by 24 160 20 120 -1.0 0.6 unbinned pixels; 3.4 2.7 nm along two shift axes; -2.0 -3.9 nm on specimen axes Total_Shift = 2.268635 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.04 microns changed by -2.24 to target drift = 0.16 nm/sec Measured defocus = -1.11 microns changed by -0.09 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3992.tif 27 frames are being saved to U:\krios\20190717\PilA_3993.tif 27 frames are being saved to U:\krios\20190717\PilA_3994.tif 27 frames are being saved to U:\krios\20190717\PilA_3995.tif 27 frames are being saved to U:\krios\20190717\PilA_3996.tif 27 frames are being saved to U:\krios\20190717\PilA_3997.tif Running script 17 at item 761 (73.23, -132.30) Remaining time to fill dewars is 28380 Dewars are NOT busy filling Trimming A by 20 165 180 20, reference by 259 22 20 225 Trimming A by 20 165 180 20, reference by 270 22 190 20 Trimming A by 20 165 180 20, reference by 23 142 20 220 Trimming A by 20 165 180 20, reference by 22 170 170 20 Disparity in stage position after aligning to center of map frame: -0.17 -0.09 Trimming A by 65 55 105 20, reference by 22 170 170 20 Disparity in stage position after aligning to target position: -0.16 -0.15 (2nd move error -0.00 -0.09) Finished aligning to item in 2 rounds Trimming A by 61 55 105 20, reference by 65 55 105 20 -13.7 2.7 unbinned pixels; 28.0 45.3 nm along two shift axes; -7.7 -54.0 nm on specimen axes Total_Shift = 28.005709 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.51 microns changed by -2.81 to target drift = 0.04 nm/sec Measured defocus = -1.34 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_3998.tif 27 frames are being saved to U:\krios\20190717\PilA_3999.tif 27 frames are being saved to U:\krios\20190717\PilA_4000.tif 27 frames are being saved to U:\krios\20190717\PilA_4001.tif 27 frames are being saved to U:\krios\20190717\PilA_4002.tif 27 frames are being saved to U:\krios\20190717\PilA_4003.tif Running script 17 at item 762 (72.13, -128.14) Remaining time to fill dewars is 28140 Dewars are NOT busy filling Trimming A by 270 23 200 20, reference by 259 22 20 225 Trimming A by 270 23 200 20, reference by 270 22 190 20 Trimming A by 270 23 200 20, reference by 23 142 20 220 Trimming A by 270 23 200 20, reference by 22 170 170 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.07 Trimming A by 155 20 135 20, reference by 270 22 190 20 Disparity in stage position after aligning to target position: -0.12 -0.12 (2nd move error -0.04 -0.06) Finished aligning to item in 2 rounds Trimming A by 155 20 135 20, reference by 155 20 135 20 -8.2 9.9 unbinned pixels; 47.0 14.5 nm along two shift axes; -36.8 -33.8 nm on specimen axes Total_Shift = 25.651047 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.44 microns changed by -2.84 to target drift = 0.07 nm/sec Measured defocus = -1.38 microns changed by -0.02 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4004.tif 27 frames are being saved to U:\krios\20190717\PilA_4005.tif 27 frames are being saved to U:\krios\20190717\PilA_4006.tif 27 frames are being saved to U:\krios\20190717\PilA_4007.tif 27 frames are being saved to U:\krios\20190717\PilA_4008.tif 27 frames are being saved to U:\krios\20190717\PilA_4009.tif Running script 17 at item 763 (69.67, -126.44) Remaining time to fill dewars is 27960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 180 22 110 20, reference by 270 22 190 20 Disparity in stage position after aligning to target position: -0.08 -0.24 (2nd move error -0.01 -0.19) Finished aligning to item in 1 rounds Trimming A by 180 20 105 20, reference by 180 22 110 20 -25.6 5.5 unbinned pixels; 53.8 83.9 nm along two shift axes; -16.1 -100.9 nm on specimen axes Total_Shift = 52.426159 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.41 microns changed by -2.91 to target drift = 0.01 nm/sec Measured defocus = -1.57 microns changed by 0.07 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4010.tif 27 frames are being saved to U:\krios\20190717\PilA_4011.tif 27 frames are being saved to U:\krios\20190717\PilA_4012.tif 27 frames are being saved to U:\krios\20190717\PilA_4013.tif 27 frames are being saved to U:\krios\20190717\PilA_4014.tif 27 frames are being saved to U:\krios\20190717\PilA_4015.tif Running script 17 at item 764 (68.08, -128.96) Remaining time to fill dewars is 27840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 100 20 45 40, reference by 270 22 190 20 Disparity in stage position after aligning to target position: -0.07 -0.25 (2nd move error 0.01 -0.20) Finished aligning to item in 1 rounds Trimming A by 100 20 40 35, reference by 100 20 45 40 -22.8 0.8 unbinned pixels; 32.9 80.9 nm along two shift axes; 1.6 -89.0 nm on specimen axes Total_Shift = 45.702819 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.22 microns changed by -2.82 to target drift = 0.08 nm/sec Measured defocus = -1.61 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4016.tif 27 frames are being saved to U:\krios\20190717\PilA_4017.tif 27 frames are being saved to U:\krios\20190717\PilA_4018.tif 27 frames are being saved to U:\krios\20190717\PilA_4019.tif 27 frames are being saved to U:\krios\20190717\PilA_4020.tif 27 frames are being saved to U:\krios\20190717\PilA_4021.tif Running script 17 at item 765 (65.37, -127.75) Remaining time to fill dewars is 27660 Dewars are NOT busy filling Trimming A by 259 22 20 225, reference by 259 22 20 225 Trimming A by 259 22 20 225, reference by 270 22 190 20 Trimming A by 259 22 20 225, reference by 23 142 20 220 Trimming A by 259 22 20 225, reference by 22 170 170 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.01 Trimming A by 150 21 20 115, reference by 259 22 20 225 Disparity in stage position after aligning to target position: 0.15 0.13 (2nd move error 0.25 0.12) Finished aligning to item in 2 rounds Trimming A by 150 20 20 110, reference by 150 21 20 115 0.2 -2.9 unbinned pixels; 10.8 3.5 nm along two shift axes; 11.1 1.5 nm on specimen axes Total_Shift = 5.75825 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.08 microns changed by -2.78 to target drift = 0.16 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4022.tif 27 frames are being saved to U:\krios\20190717\PilA_4023.tif 27 frames are being saved to U:\krios\20190717\PilA_4024.tif 27 frames are being saved to U:\krios\20190717\PilA_4025.tif 27 frames are being saved to U:\krios\20190717\PilA_4026.tif 27 frames are being saved to U:\krios\20190717\PilA_4027.tif Running script 17 at item 766 (70.62, -130.53) Remaining time to fill dewars is 27420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 80 20 75 20, reference by 270 22 190 20 Disparity in stage position after aligning to target position: 0.05 -0.14 (2nd move error 0.12 -0.09) Finished aligning to item in 1 rounds Trimming A by 85 24 75 20, reference by 80 20 75 20 -10.0 -0.7 unbinned pixels; 10.7 37.2 nm along two shift axes; 4.7 -39.0 nm on specimen axes Total_Shift = 20.139748 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.92 microns changed by -2.72 to target drift = 0.13 nm/sec Measured defocus = -1.76 microns changed by -0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4028.tif 27 frames are being saved to U:\krios\20190717\PilA_4029.tif 27 frames are being saved to U:\krios\20190717\PilA_4030.tif 27 frames are being saved to U:\krios\20190717\PilA_4031.tif 27 frames are being saved to U:\krios\20190717\PilA_4032.tif 27 frames are being saved to U:\krios\20190717\PilA_4033.tif Running script 17 at item 767 (71.60, -134.90) Remaining time to fill dewars is 27420 Dewars are NOT busy filling Trimming A by 22 170 175 20, reference by 259 22 20 225 Trimming A by 22 170 175 20, reference by 270 22 190 20 Trimming A by 22 170 175 20, reference by 23 142 20 220 Trimming A by 22 170 175 20, reference by 22 170 170 20 Disparity in stage position after aligning to center of map frame: -0.13 -0.07 Trimming A by 23 57 100 20, reference by 22 170 170 20 Disparity in stage position after aligning to target position: -0.17 -0.22 (2nd move error -0.04 -0.18) Finished aligning to item in 2 rounds Trimming A by 20 66 95 20, reference by 23 57 100 20 -22.1 9.4 unbinned pixels; 63.5 65.5 nm along two shift axes; -32.0 -88.1 nm on specimen axes Total_Shift = 48.113067 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.86 microns changed by -2.76 to target drift = 0.02 nm/sec Measured defocus = -1.90 microns changed by 0.00 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4034.tif 27 frames are being saved to U:\krios\20190717\PilA_4035.tif 27 frames are being saved to U:\krios\20190717\PilA_4036.tif 27 frames are being saved to U:\krios\20190717\PilA_4037.tif 27 frames are being saved to U:\krios\20190717\PilA_4038.tif 27 frames are being saved to U:\krios\20190717\PilA_4039.tif Running script 17 at item 768 (69.10, -133.18) Remaining time to fill dewars is 27240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 22 170 170 20 Disparity in stage position after aligning to target position: -0.28 -0.23 (2nd move error -0.16 -0.19) Finished aligning to item in 1 rounds -18.9 32.6 unbinned pixels; 144.6 18.8 nm along two shift axes; -123.0 -80.3 nm on specimen axes Total_Shift = 75.384903 1.7 21.4 unbinned pixels; 76.4 38.2 nm along two shift axes; -83.5 2.0 nm on specimen axes Total_Shift = 42.882224 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.80 microns changed by -2.80 to target drift = 0.02 nm/sec Measured defocus = -2.08 microns changed by 0.08 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4040.tif 27 frames are being saved to U:\krios\20190717\PilA_4041.tif 27 frames are being saved to U:\krios\20190717\PilA_4042.tif 27 frames are being saved to U:\krios\20190717\PilA_4043.tif 27 frames are being saved to U:\krios\20190717\PilA_4044.tif 27 frames are being saved to U:\krios\20190717\PilA_4045.tif Running script 17 at item 769 (66.56, -131.99) Remaining time to fill dewars is 27120 Dewars are NOT busy filling Trimming A by 24 147 20 215, reference by 259 22 20 225 Trimming A by 24 147 20 215, reference by 270 22 190 20 Trimming A by 24 147 20 215, reference by 23 142 20 220 Trimming A by 24 147 20 215, reference by 22 170 170 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.05 Trimming A by 40 20 20 55, reference by 23 142 20 220 Disparity in stage position after aligning to target position: -0.08 0.14 (2nd move error 0.01 0.16) Finished aligning to item in 2 rounds Trimming A by 45 21 20 55, reference by 40 20 20 55 -0.8 0.3 unbinned pixels; 2.2 2.4 nm along two shift axes; -1.1 -3.2 nm on specimen axes Total_Shift = 1.745793 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.62 microns changed by -2.72 to target drift = 0.12 nm/sec Measured defocus = -2.14 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4046.tif 27 frames are being saved to U:\krios\20190717\PilA_4047.tif 27 frames are being saved to U:\krios\20190717\PilA_4048.tif 27 frames are being saved to U:\krios\20190717\PilA_4049.tif 27 frames are being saved to U:\krios\20190717\PilA_4050.tif 27 frames are being saved to U:\krios\20190717\PilA_4051.tif Running script 17 at item 770 (62.46, -132.81) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 66 30 20 145, reference by 23 142 20 220 Disparity in stage position after aligning to target position: -0.10 -0.02 (2nd move error -0.01 0.00) Finished aligning to item in 1 rounds Trimming A by 66 30 20 145, reference by 66 30 20 145 -0.9 3.4 unbinned pixels; 13.7 2.0 nm along two shift axes; -13.1 -4.1 nm on specimen axes Total_Shift = 7.039466 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.48 microns changed by -2.68 to target drift = 0.07 nm/sec Measured defocus = -2.23 microns changed by 0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4052.tif 27 frames are being saved to U:\krios\20190717\PilA_4053.tif 27 frames are being saved to U:\krios\20190717\PilA_4054.tif 27 frames are being saved to U:\krios\20190717\PilA_4055.tif 27 frames are being saved to U:\krios\20190717\PilA_4056.tif 27 frames are being saved to U:\krios\20190717\PilA_4057.tif Running script 17 at item 771 (65.00, -134.58) Remaining time to fill dewars is 26700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 45 20 100, reference by 23 142 20 220 Disparity in stage position after aligning to target position: -0.05 0.08 (2nd move error 0.04 0.10) Finished aligning to item in 1 rounds Trimming A by 21 40 20 100, reference by 20 45 20 100 -0.5 -2.3 unbinned pixels; 7.8 5.1 nm along two shift axes; 9.0 -1.4 nm on specimen axes Total_Shift = 4.668788 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.27 microns changed by -2.57 to target drift = 0.13 nm/sec Measured defocus = -2.30 microns changed by 0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4058.tif 27 frames are being saved to U:\krios\20190717\PilA_4059.tif 27 frames are being saved to U:\krios\20190717\PilA_4060.tif 27 frames are being saved to U:\krios\20190717\PilA_4061.tif 27 frames are being saved to U:\krios\20190717\PilA_4062.tif 27 frames are being saved to U:\krios\20190717\PilA_4063.tif Running script 17 at item 772 (67.50, -136.25) Remaining time to fill dewars is 26580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 75 20 70, reference by 23 142 20 220 Disparity in stage position after aligning to target position: 0.06 0.16 (2nd move error 0.14 0.19) Finished aligning to item in 1 rounds Trimming A by 21 70 45 60, reference by 20 75 20 70 2.9 2.2 unbinned pixels; 4.3 13.8 nm along two shift axes; -9.2 10.8 nm on specimen axes Total_Shift = 7.284998 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.25 microns changed by -2.65 to target drift = 0.16 nm/sec Measured defocus = -2.39 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4064.tif 27 frames are being saved to U:\krios\20190717\PilA_4065.tif 27 frames are being saved to U:\krios\20190717\PilA_4066.tif 27 frames are being saved to U:\krios\20190717\PilA_4067.tif 27 frames are being saved to U:\krios\20190717\PilA_4068.tif 27 frames are being saved to U:\krios\20190717\PilA_4069.tif Running script 17 at item 773 (69.98, -137.52) Remaining time to fill dewars is 26400 Dewars are NOT busy filling Trimming A by 22 170 175 20, reference by 259 22 20 225 Trimming A by 22 170 175 20, reference by 270 22 190 20 Trimming A by 22 170 175 20, reference by 23 142 20 220 Trimming A by 22 170 175 20, reference by 22 170 170 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.04 Trimming A by 22 100 70 20, reference by 22 170 170 20 Disparity in stage position after aligning to target position: -0.06 -0.25 (2nd move error 0.04 -0.23) Finished aligning to item in 2 rounds Trimming A by 22 105 70 20, reference by 22 100 70 20 -24.3 -3.1 unbinned pixels; 20.4 92.1 nm along two shift axes; 17.1 -93.9 nm on specimen axes Total_Shift = 48.991523 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -1.10 microns changed by -1.40 to target drift = 0.16 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4070.tif 27 frames are being saved to U:\krios\20190717\PilA_4071.tif 27 frames are being saved to U:\krios\20190717\PilA_4072.tif 27 frames are being saved to U:\krios\20190717\PilA_4073.tif 27 frames are being saved to U:\krios\20190717\PilA_4074.tif 27 frames are being saved to U:\krios\20190717\PilA_4075.tif Running script 17 at item 774 (68.31, -140.08) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 160 25 100, reference by 22 170 170 20 Disparity in stage position after aligning to target position: -0.06 -0.29 (2nd move error 0.04 -0.28) Finished aligning to item in 1 rounds Trimming A by 21 165 85 100, reference by 20 160 25 100 -28.4 -0.3 unbinned pixels; 36.1 102.6 nm along two shift axes; 7.0 -110.4 nm on specimen axes Total_Shift = 56.784044 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.11 microns changed by -2.71 to target drift = 0.13 nm/sec Measured defocus = -2.67 microns changed by 0.07 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4076.tif 27 frames are being saved to U:\krios\20190717\PilA_4077.tif 27 frames are being saved to U:\krios\20190717\PilA_4078.tif 27 frames are being saved to U:\krios\20190717\PilA_4079.tif 27 frames are being saved to U:\krios\20190717\PilA_4080.tif 27 frames are being saved to U:\krios\20190717\PilA_4081.tif Running script 17 at item 775 (65.82, -138.90) Remaining time to fill dewars is 26100 Dewars are NOT busy filling Trimming A by 24 147 20 215, reference by 259 22 20 225 Trimming A by 24 147 20 215, reference by 270 22 190 20 Trimming A by 24 147 20 215, reference by 23 142 20 220 Trimming A by 24 147 20 215, reference by 22 170 170 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.05 Trimming A by 22 150 20 130, reference by 23 142 20 220 Disparity in stage position after aligning to target position: 0.10 0.12 (2nd move error 0.15 0.14) Finished aligning to item in 2 rounds Trimming A by 22 145 20 130, reference by 22 150 20 130 1.5 2.2 unbinned pixels; 6.2 8.9 nm along two shift axes; -9.0 5.5 nm on specimen axes Total_Shift = 5.422257 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 0.51 microns changed by -1.41 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4082.tif 27 frames are being saved to U:\krios\20190717\PilA_4083.tif 27 frames are being saved to U:\krios\20190717\PilA_4084.tif 27 frames are being saved to U:\krios\20190717\PilA_4085.tif 27 frames are being saved to U:\krios\20190717\PilA_4086.tif 27 frames are being saved to U:\krios\20190717\PilA_4087.tif Running script 17 at item 776 (63.32, -137.15) Remaining time to fill dewars is 25860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 116 20 165, reference by 23 142 20 220 Disparity in stage position after aligning to target position: 0.02 0.01 (2nd move error 0.07 0.03) Finished aligning to item in 1 rounds Trimming A by 23 111 20 165, reference by 21 116 20 165 -1.0 3.5 unbinned pixels; 14.0 1.6 nm along two shift axes; -13.3 -4.6 nm on specimen axes Total_Shift = 7.209369 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.74 microns changed by -2.74 to target drift = 0.12 nm/sec Measured defocus = -1.01 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4088.tif 27 frames are being saved to U:\krios\20190717\PilA_4089.tif 27 frames are being saved to U:\krios\20190717\PilA_4090.tif 27 frames are being saved to U:\krios\20190717\PilA_4091.tif 27 frames are being saved to U:\krios\20190717\PilA_4092.tif 27 frames are being saved to U:\krios\20190717\PilA_4093.tif Running script 17 at item 777 (580.05, -491.11) Remaining time to fill dewars is 25740 Dewars are NOT busy filling Trimming A by 210 24 160 20, reference by 221 24 20 260 Trimming A by 210 24 160 20, reference by 205 24 155 20 Trimming A by 210 24 160 20, reference by 23 210 20 240 Trimming A by 210 24 160 20, reference by 23 171 155 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.05 Trimming A by 115 21 155 30, reference by 205 24 155 20 Disparity in stage position after aligning to target position: -0.15 0.01 (2nd move error 0.07 0.03) Finished aligning to item in 2 rounds Trimming A by 115 21 160 35, reference by 115 21 155 30 -1.0 -0.1 unbinned pixels; 1.0 3.9 nm along two shift axes; 0.6 -4.0 nm on specimen axes Total_Shift = 2.078446 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 3.03 microns changed by -4.13 to target drift = 0.21 nm/sec Measured defocus = -1.13 microns changed by 0.03 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4094.tif 27 frames are being saved to U:\krios\20190717\PilA_4095.tif 27 frames are being saved to U:\krios\20190717\PilA_4096.tif 27 frames are being saved to U:\krios\20190717\PilA_4097.tif 27 frames are being saved to U:\krios\20190717\PilA_4098.tif 27 frames are being saved to U:\krios\20190717\PilA_4099.tif Running script 17 at item 778 (577.60, -489.42) Remaining time to fill dewars is 25680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 21 125 20, reference by 205 24 155 20 Disparity in stage position after aligning to target position: -0.17 -0.14 (2nd move error 0.05 -0.11) Finished aligning to item in 1 rounds Trimming A by 135 21 125 20, reference by 135 21 125 20 -12.6 -4.6 unbinned pixels; 0.3 52.4 nm along two shift axes; 20.5 -48.2 nm on specimen axes Total_Shift = 26.864956 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.39 microns changed by -2.59 to target drift = 0.07 nm/sec Measured defocus = -1.18 microns changed by -0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4100.tif 27 frames are being saved to U:\krios\20190717\PilA_4101.tif 27 frames are being saved to U:\krios\20190717\PilA_4102.tif 27 frames are being saved to U:\krios\20190717\PilA_4103.tif 27 frames are being saved to U:\krios\20190717\PilA_4104.tif 27 frames are being saved to U:\krios\20190717\PilA_4105.tif Running script 17 at item 779 (575.13, -487.66) Remaining time to fill dewars is 25440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 23 105 20, reference by 205 24 155 20 Disparity in stage position after aligning to target position: -0.13 -0.23 (2nd move error 0.09 -0.20) Finished aligning to item in 1 rounds Trimming A by 150 23 100 20, reference by 150 23 105 20 -23.6 -4.0 unbinned pixels; 16.0 91.1 nm along two shift axes; 20.7 -91.1 nm on specimen axes Total_Shift = 47.931591 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.45 microns changed by -3.75 to target drift = 0.06 nm/sec Measured defocus = -1.33 microns changed by 0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4106.tif 27 frames are being saved to U:\krios\20190717\PilA_4107.tif 27 frames are being saved to U:\krios\20190717\PilA_4108.tif 27 frames are being saved to U:\krios\20190717\PilA_4109.tif 27 frames are being saved to U:\krios\20190717\PilA_4110.tif 27 frames are being saved to U:\krios\20190717\PilA_4111.tif Running script 17 at item 780 (570.85, -488.96) Remaining time to fill dewars is 25320 Dewars are NOT busy filling Trimming A by 225 24 20 255, reference by 221 24 20 260 Trimming A by 225 24 20 255, reference by 205 24 155 20 Trimming A by 225 24 20 255, reference by 23 210 20 240 Trimming A by 225 24 20 255, reference by 23 171 155 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.04 Trimming A by 125 23 20 105, reference by 221 24 20 260 Disparity in stage position after aligning to target position: 0.05 0.24 (2nd move error 0.14 0.26) Finished aligning to item in 2 rounds Trimming A by 130 23 20 105, reference by 125 23 20 105 5.1 6.8 unbinned pixels; 18.4 28.6 nm along two shift axes; -27.6 18.4 nm on specimen axes Total_Shift = 17.021398 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.82 microns changed by -2.22 to target drift = 0.15 nm/sec Measured defocus = -1.36 microns changed by -0.04 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4112.tif 27 frames are being saved to U:\krios\20190717\PilA_4113.tif 27 frames are being saved to U:\krios\20190717\PilA_4114.tif 27 frames are being saved to U:\krios\20190717\PilA_4115.tif 27 frames are being saved to U:\krios\20190717\PilA_4116.tif 27 frames are being saved to U:\krios\20190717\PilA_4117.tif Running script 17 at item 781 (573.54, -490.22) Remaining time to fill dewars is 25200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 75 20 45 35, reference by 205 24 155 20 Disparity in stage position after aligning to target position: -0.02 -0.31 (2nd move error 0.21 -0.28) Finished aligning to item in 1 rounds Trimming A by 70 20 45 25, reference by 75 20 45 35 -24.3 5.9 unbinned pixels; 53.3 78.7 nm along two shift axes; -17.7 -95.8 nm on specimen axes Total_Shift = 50.009936 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.26 microns changed by -3.76 to target drift = 0.08 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4118.tif 27 frames are being saved to U:\krios\20190717\PilA_4119.tif 27 frames are being saved to U:\krios\20190717\PilA_4120.tif 27 frames are being saved to U:\krios\20190717\PilA_4121.tif 27 frames are being saved to U:\krios\20190717\PilA_4122.tif 27 frames are being saved to U:\krios\20190717\PilA_4123.tif Running script 17 at item 782 (575.95, -491.95) Remaining time to fill dewars is 25080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 60 20 55 20, reference by 205 24 155 20 Disparity in stage position after aligning to target position: 0.02 -0.21 (2nd move error 0.24 -0.18) Finished aligning to item in 1 rounds Trimming A by 60 20 60 20, reference by 60 20 55 20 -16.7 -5.9 unbinned pixels; 0.0 68.9 nm along two shift axes; 26.5 -63.6 nm on specimen axes Total_Shift = 35.345818 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.00 microns changed by -2.60 to target drift = 0.16 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4124.tif 27 frames are being saved to U:\krios\20190717\PilA_4125.tif 27 frames are being saved to U:\krios\20190717\PilA_4126.tif 27 frames are being saved to U:\krios\20190717\PilA_4127.tif 27 frames are being saved to U:\krios\20190717\PilA_4128.tif 27 frames are being saved to U:\krios\20190717\PilA_4129.tif Running script 17 at item 783 (578.67, -493.58) Remaining time to fill dewars is 24900 Dewars are NOT busy filling Trimming A by 23 167 160 20, reference by 221 24 20 260 Trimming A by 23 167 160 20, reference by 205 24 155 20 Trimming A by 23 167 160 20, reference by 23 210 20 240 Trimming A by 23 167 160 20, reference by 23 171 155 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.11 Trimming A by 23 38 125 20, reference by 23 171 155 20 Disparity in stage position after aligning to target position: -0.18 -0.12 (2nd move error -0.07 -0.03) Finished aligning to item in 2 rounds Trimming A by 28 47 120 20, reference by 23 38 125 20 -6.2 25.4 unbinned pixels; 101.6 15.9 nm along two shift axes; -97.6 -29.5 nm on specimen axes Total_Shift = 52.36082 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 2.38 microns changed by -4.08 to target drift = 0.11 nm/sec Measured defocus = -1.71 microns changed by 0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4130.tif 27 frames are being saved to U:\krios\20190717\PilA_4131.tif 27 frames are being saved to U:\krios\20190717\PilA_4132.tif 27 frames are being saved to U:\krios\20190717\PilA_4133.tif 27 frames are being saved to U:\krios\20190717\PilA_4134.tif 27 frames are being saved to U:\krios\20190717\PilA_4135.tif Running script 17 at item 784 (579.53, -497.89) Remaining time to fill dewars is 24720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 155 160 20, reference by 23 171 155 20 Disparity in stage position after aligning to target position: -0.16 -0.06 (2nd move error -0.05 0.03) Finished aligning to item in 1 rounds Trimming A by 20 142 170 20, reference by 22 155 160 20 1.2 10.4 unbinned pixels; 36.8 19.9 nm along two shift axes; -40.8 2.4 nm on specimen axes Total_Shift = 20.997196 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.15 microns changed by -2.95 to target drift = 0.16 nm/sec Measured defocus = -1.84 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4136.tif 27 frames are being saved to U:\krios\20190717\PilA_4137.tif 27 frames are being saved to U:\krios\20190717\PilA_4138.tif 27 frames are being saved to U:\krios\20190717\PilA_4139.tif 27 frames are being saved to U:\krios\20190717\PilA_4140.tif 27 frames are being saved to U:\krios\20190717\PilA_4141.tif Running script 17 at item 785 (577.01, -496.15) Remaining time to fill dewars is 24600 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 22 72 100 20, reference by 23 171 155 20 Disparity in stage position after aligning to target position: -0.24 -0.22 (2nd move error -0.13 -0.13) Finished aligning to item in 1 rounds Trimming A by 22 72 100 20, reference by 22 72 100 20 -14.1 24.0 unbinned pixels; 106.8 14.5 nm along two shift axes; -90.6 -59.9 nm on specimen axes Total_Shift = 55.749973 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 2.13 microns changed by -4.03 to target drift = 0.08 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4142.tif 27 frames are being saved to U:\krios\20190717\PilA_4143.tif 27 frames are being saved to U:\krios\20190717\PilA_4144.tif 27 frames are being saved to U:\krios\20190717\PilA_4145.tif 27 frames are being saved to U:\krios\20190717\PilA_4146.tif 27 frames are being saved to U:\krios\20190717\PilA_4147.tif Running script 17 at item 786 (574.53, -494.39) Remaining time to fill dewars is 24360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 171 155 20 Disparity in stage position after aligning to target position: -0.23 -0.36 (2nd move error -0.12 -0.28) Finished aligning to item in 1 rounds -33.0 25.3 unbinned pixels; 136.1 80.8 nm along two shift axes; -91.4 -133.8 nm on specimen axes Total_Shift = 83.190454 -3.0 15.7 unbinned pixels; 61.7 13.0 nm along two shift axes; -60.6 -14.9 nm on specimen axes Total_Shift = 32.019783 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.57 microns changed by -2.57 to target drift = 0.05 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4148.tif 27 frames are being saved to U:\krios\20190717\PilA_4149.tif 27 frames are being saved to U:\krios\20190717\PilA_4150.tif 27 frames are being saved to U:\krios\20190717\PilA_4151.tif 27 frames are being saved to U:\krios\20190717\PilA_4152.tif 27 frames are being saved to U:\krios\20190717\PilA_4153.tif Running script 17 at item 787 (571.89, -492.68) Remaining time to fill dewars is 24300 Dewars are NOT busy filling Trimming A by 210 24 160 20, reference by 221 24 20 260 Trimming A by 210 24 160 20, reference by 205 24 155 20 Trimming A by 210 24 160 20, reference by 23 210 20 240 Trimming A by 210 24 160 20, reference by 23 171 155 20 Disparity in stage position after aligning to center of map frame: -0.07 -0.10 Trimming A by 30 20 20 55, reference by 205 24 155 20 Disparity in stage position after aligning to target position: -0.05 -0.42 (2nd move error 0.02 -0.34) Finished aligning to item in 2 rounds Trimming A by 30 20 20 50, reference by 30 20 20 55 -31.2 -2.7 unbinned pixels; 30.8 116.3 nm along two shift axes; 17.0 -120.7 nm on specimen axes Total_Shift = 62.584482 Trimming A by 35 21 20 45, reference by 30 20 20 55 -4.7 -4.5 unbinned pixels; 10.3 23.6 nm along two shift axes; 18.4 -17.3 nm on specimen axes Total_Shift = 12.966456 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.64 microns changed by -3.74 to target drift = 0.13 nm/sec Measured defocus = -2.16 microns changed by 0.06 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4154.tif 27 frames are being saved to U:\krios\20190717\PilA_4155.tif 27 frames are being saved to U:\krios\20190717\PilA_4156.tif 27 frames are being saved to U:\krios\20190717\PilA_4157.tif 27 frames are being saved to U:\krios\20190717\PilA_4158.tif 27 frames are being saved to U:\krios\20190717\PilA_4159.tif Running script 17 at item 788 (569.28, -491.64) Remaining time to fill dewars is 24060 Dewars are NOT busy filling Trimming A by 221 24 20 260, reference by 221 24 20 260 Trimming A by 221 24 20 260, reference by 205 24 155 20 Trimming A by 221 24 20 260, reference by 23 210 20 240 Trimming A by 221 24 20 260, reference by 23 171 155 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.06 Trimming A by 68 24 20 135, reference by 221 24 20 260 Disparity in stage position after aligning to target position: 0.10 0.11 (2nd move error 0.13 0.16) Finished aligning to item in 2 rounds Trimming A by 68 24 20 135, reference by 68 24 20 135 -1.2 0.4 unbinned pixels; 3.1 3.8 nm along two shift axes; -1.3 -4.8 nm on specimen axes Total_Shift = 2.573002 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.02 microns changed by -2.22 to target drift = 0.13 nm/sec Measured defocus = -2.24 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4160.tif 27 frames are being saved to U:\krios\20190717\PilA_4161.tif 27 frames are being saved to U:\krios\20190717\PilA_4162.tif 27 frames are being saved to U:\krios\20190717\PilA_4163.tif 27 frames are being saved to U:\krios\20190717\PilA_4164.tif 27 frames are being saved to U:\krios\20190717\PilA_4165.tif Running script 17 at item 789 (567.76, -494.14) Remaining time to fill dewars is 23880 Dewars are NOT busy filling Trimming A by 24 205 20 240, reference by 221 24 20 260 Trimming A by 24 205 20 240, reference by 205 24 155 20 Trimming A by 24 205 20 240, reference by 23 210 20 240 Trimming A by 24 205 20 240, reference by 23 171 155 20 Disparity in stage position after aligning to center of map frame: -0.03 -0.09 Trimming A by 55 37 20 145, reference by 23 210 20 240 Disparity in stage position after aligning to target position: -0.01 0.07 (2nd move error 0.01 0.14) Finished aligning to item in 2 rounds Trimming A by 51 25 20 150, reference by 55 37 20 145 -0.8 0.9 unbinned pixels; 4.4 1.3 nm along two shift axes; -3.4 -3.2 nm on specimen axes Total_Shift = 2.398454 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.20 microns changed by -3.50 to target drift = 0.14 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4166.tif 27 frames are being saved to U:\krios\20190717\PilA_4167.tif 27 frames are being saved to U:\krios\20190717\PilA_4168.tif 27 frames are being saved to U:\krios\20190717\PilA_4169.tif 27 frames are being saved to U:\krios\20190717\PilA_4170.tif 27 frames are being saved to U:\krios\20190717\PilA_4171.tif Running script 17 at item 790 (570.29, -495.86) Remaining time to fill dewars is 23640 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 75 20 95, reference by 23 210 20 240 Disparity in stage position after aligning to target position: 0.09 0.10 (2nd move error 0.12 0.17) Finished aligning to item in 1 rounds Trimming A by 21 70 20 90, reference by 20 75 20 95 -2.5 1.7 unbinned pixels; 9.6 6.4 nm along two shift axes; -6.2 -10.1 nm on specimen axes Total_Shift = 6.065452 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 3.49 microns changed by -5.89 to target drift = 1.65 nm/sec Measured defocus = -6.08 microns changed by 3.68 to target drift = 0.06 nm/sec Measured defocus = -4.04 microns changed by 1.64 to target drift = 0.05 nm/sec Measured defocus = -2.52 microns changed by 0.12 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4172.tif 27 frames are being saved to U:\krios\20190717\PilA_4173.tif 27 frames are being saved to U:\krios\20190717\PilA_4174.tif 27 frames are being saved to U:\krios\20190717\PilA_4175.tif 27 frames are being saved to U:\krios\20190717\PilA_4176.tif 27 frames are being saved to U:\krios\20190717\PilA_4177.tif Running script 17 at item 791 (575.38, -498.72) Remaining time to fill dewars is 23520 Dewars are NOT busy filling Trimming A by 23 167 160 20, reference by 221 24 20 260 Trimming A by 23 167 160 20, reference by 205 24 155 20 Trimming A by 23 167 160 20, reference by 23 210 20 240 Trimming A by 23 167 160 20, reference by 23 171 155 20 Disparity in stage position after aligning to center of map frame: -0.07 -0.10 Trimming A by 20 110 70 35, reference by 23 171 155 20 Disparity in stage position after aligning to target position: -0.11 -0.29 (2nd move error -0.04 -0.21) Finished aligning to item in 2 rounds Trimming A by 20 110 70 40, reference by 20 110 70 35 -27.5 8.0 unbinned pixels; 65.3 86.8 nm along two shift axes; -25.4 -108.6 nm on specimen axes Total_Shift = 57.234784 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.52 microns changed by -4.02 to target drift = 0.01 nm/sec Measured defocus = -2.55 microns changed by 0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4178.tif 27 frames are being saved to U:\krios\20190717\PilA_4179.tif 27 frames are being saved to U:\krios\20190717\PilA_4180.tif 27 frames are being saved to U:\krios\20190717\PilA_4181.tif 27 frames are being saved to U:\krios\20190717\PilA_4182.tif 27 frames are being saved to U:\krios\20190717\PilA_4183.tif Running script 17 at item 792 (424.78, -259.83) Remaining time to fill dewars is 23280 Dewars are NOT busy filling Trimming A by 22 165 205 20, reference by 288 21 20 245 Trimming A by 22 165 205 20, reference by 313 20 250 20 Trimming A by 22 165 205 20, reference by 22 145 20 190 Trimming A by 22 165 205 20, reference by 20 152 210 20 Disparity in stage position after aligning to center of map frame: -0.13 -0.08 Trimming A by 53 61 140 20, reference by 20 152 210 20 Disparity in stage position after aligning to target position: -0.16 -0.19 (2nd move error -0.04 -0.13) Finished aligning to item in 2 rounds Trimming A by 45 56 145 20, reference by 53 61 140 20 -16.4 8.1 unbinned pixels; 51.2 46.7 nm along two shift axes; -28.1 -65.3 nm on specimen axes Total_Shift = 36.522151 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.33 microns changed by -2.27 to target drift = 0.18 nm/sec Measured defocus = -2.61 microns changed by 0.01 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4184.tif 27 frames are being saved to U:\krios\20190717\PilA_4185.tif 27 frames are being saved to U:\krios\20190717\PilA_4186.tif 27 frames are being saved to U:\krios\20190717\PilA_4187.tif 27 frames are being saved to U:\krios\20190717\PilA_4188.tif 27 frames are being saved to U:\krios\20190717\PilA_4189.tif Running script 17 at item 793 (422.23, -258.14) Remaining time to fill dewars is 23160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 67 24 105 20, reference by 20 152 210 20 Disparity in stage position after aligning to target position: -0.13 -0.33 (2nd move error -0.00 -0.28) Finished aligning to item in 1 rounds Trimming A by 80 20 85 20, reference by 67 24 105 20 -32.2 4.4 unbinned pixels; 58.0 109.3 nm along two shift axes; -10.2 -126.1 nm on specimen axes Total_Shift = 64.944472 Trimming A by 80 20 85 20, reference by 67 24 105 20 -5.0 2.1 unbinned pixels; 14.3 14.8 nm along two shift axes; -7.2 -19.9 nm on specimen axes Total_Shift = 10.853546 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.68 microns changed by -3.58 to target drift = 0.03 nm/sec Measured defocus = -0.94 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4190.tif 27 frames are being saved to U:\krios\20190717\PilA_4191.tif 27 frames are being saved to U:\krios\20190717\PilA_4192.tif 27 frames are being saved to U:\krios\20190717\PilA_4193.tif 27 frames are being saved to U:\krios\20190717\PilA_4194.tif 27 frames are being saved to U:\krios\20190717\PilA_4195.tif Running script 17 at item 794 (419.56, -256.52) Remaining time to fill dewars is 22920 Dewars are NOT busy filling Trimming A by 305 20 20 230, reference by 288 21 20 245 Trimming A by 305 20 20 230, reference by 313 20 250 20 Trimming A by 305 20 20 230, reference by 22 145 20 190 Trimming A by 305 20 20 230, reference by 20 152 210 20 Disparity in stage position after aligning to center of map frame: -0.03 -0.07 Trimming A by 100 20 60 45, reference by 288 21 20 245 Disparity in stage position after aligning to target position: 0.14 0.26 (2nd move error 0.16 0.31) Finished aligning to item in 2 rounds Trimming A by 110 20 65 40, reference by 100 20 60 45 2.1 -2.1 unbinned pixels; 10.5 4.5 nm along two shift axes; 7.8 8.7 nm on specimen axes Total_Shift = 5.999591 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.43 microns changed by -3.43 to target drift = 0.15 nm/sec Measured defocus = -1.01 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4196.tif 27 frames are being saved to U:\krios\20190717\PilA_4197.tif 27 frames are being saved to U:\krios\20190717\PilA_4198.tif 27 frames are being saved to U:\krios\20190717\PilA_4199.tif 27 frames are being saved to U:\krios\20190717\PilA_4200.tif 27 frames are being saved to U:\krios\20190717\PilA_4201.tif Running script 17 at item 795 (416.98, -255.27) Remaining time to fill dewars is 22620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 155 20 20 120, reference by 288 21 20 245 Disparity in stage position after aligning to target position: 0.20 0.04 (2nd move error 0.22 0.09) Finished aligning to item in 1 rounds Trimming A by 150 22 20 120, reference by 155 20 20 120 1.4 -5.4 unbinned pixels; 21.8 3.0 nm along two shift axes; 20.8 6.7 nm on specimen axes Total_Shift = 11.216481 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.17 microns changed by -3.27 to target drift = 0.13 nm/sec Measured defocus = -1.07 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4202.tif 27 frames are being saved to U:\krios\20190717\PilA_4203.tif 27 frames are being saved to U:\krios\20190717\PilA_4204.tif 27 frames are being saved to U:\krios\20190717\PilA_4205.tif 27 frames are being saved to U:\krios\20190717\PilA_4206.tif 27 frames are being saved to U:\krios\20190717\PilA_4207.tif Running script 17 at item 796 (415.69, -257.89) Remaining time to fill dewars is 22440 Dewars are NOT busy filling Trimming A by 23 137 20 200, reference by 288 21 20 245 Trimming A by 23 137 20 200, reference by 313 20 250 20 Trimming A by 23 137 20 200, reference by 22 145 20 190 Trimming A by 23 137 20 200, reference by 20 152 210 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.07 Trimming A by 87 24 20 145, reference by 22 145 20 190 Disparity in stage position after aligning to target position: -0.13 0.07 (2nd move error -0.16 0.11) Finished aligning to item in 2 rounds Trimming A by 105 21 20 135, reference by 87 24 20 145 -1.4 30.3 unbinned pixels; 113.4 40.6 nm along two shift axes; -117.8 -11.8 nm on specimen axes Total_Shift = 60.760714 Trimming A by 96 21 20 145, reference by 87 24 20 145 -1.6 18.7 unbinned pixels; 70.9 22.6 nm along two shift axes; -72.5 -10.0 nm on specimen axes Total_Shift = 37.56193 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.96 microns changed by -3.16 to target drift = 0.14 nm/sec Measured defocus = -1.16 microns changed by -0.04 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4208.tif 27 frames are being saved to U:\krios\20190717\PilA_4209.tif 27 frames are being saved to U:\krios\20190717\PilA_4210.tif 27 frames are being saved to U:\krios\20190717\PilA_4211.tif 27 frames are being saved to U:\krios\20190717\PilA_4212.tif 27 frames are being saved to U:\krios\20190717\PilA_4213.tif Running script 17 at item 797 (418.24, -259.72) Remaining time to fill dewars is 22260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 21 20 60, reference by 22 145 20 190 Disparity in stage position after aligning to target position: -0.02 0.17 (2nd move error -0.05 0.22) Finished aligning to item in 1 rounds Trimming A by 31 21 20 70, reference by 35 21 20 60 -3.1 13.5 unbinned pixels; 53.8 9.1 nm along two shift axes; -52.0 -15.0 nm on specimen axes Total_Shift = 27.778841 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.01 microns changed by -3.31 to target drift = 0.08 nm/sec Measured defocus = -1.28 microns changed by -0.02 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4214.tif 27 frames are being saved to U:\krios\20190717\PilA_4215.tif 27 frames are being saved to U:\krios\20190717\PilA_4216.tif 27 frames are being saved to U:\krios\20190717\PilA_4217.tif 27 frames are being saved to U:\krios\20190717\PilA_4218.tif 27 frames are being saved to U:\krios\20190717\PilA_4219.tif Running script 17 at item 798 (420.61, -260.71) Remaining time to fill dewars is 22140 Dewars are NOT busy filling Trimming A by 21 165 200 20, reference by 288 21 20 245 Trimming A by 21 165 200 20, reference by 313 20 250 20 Trimming A by 21 165 200 20, reference by 22 145 20 190 Trimming A by 21 165 200 20, reference by 20 152 210 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.07 Trimming A by 20 40 35 20, reference by 20 152 210 20 Disparity in stage position after aligning to target position: 0.01 -0.38 (2nd move error 0.00 -0.33) Finished aligning to item in 2 rounds Trimming A by 21 40 40 20, reference by 20 40 35 20 -28.2 2.6 unbinned pixels; 46.4 97.5 nm along two shift axes; -4.2 -110.2 nm on specimen axes Total_Shift = 56.617419 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.11 microns changed by -3.51 to target drift = 0.06 nm/sec Measured defocus = -1.48 microns changed by 0.08 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4220.tif 27 frames are being saved to U:\krios\20190717\PilA_4221.tif 27 frames are being saved to U:\krios\20190717\PilA_4222.tif 27 frames are being saved to U:\krios\20190717\PilA_4223.tif 27 frames are being saved to U:\krios\20190717\PilA_4224.tif 27 frames are being saved to U:\krios\20190717\PilA_4225.tif Running script 17 at item 799 (423.10, -262.46) Remaining time to fill dewars is 21900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 87 120 20, reference by 20 152 210 20 Disparity in stage position after aligning to target position: 0.08 -0.27 (2nd move error 0.07 -0.22) Finished aligning to item in 1 rounds Trimming A by 23 110 105 20, reference by 22 87 120 20 -25.9 0.7 unbinned pixels; 36.5 92.2 nm along two shift axes; 2.6 -100.9 nm on specimen axes Total_Shift = 51.830837 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.08 microns changed by -3.58 to target drift = 0.09 nm/sec Measured defocus = -1.59 microns changed by 0.09 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4226.tif 27 frames are being saved to U:\krios\20190717\PilA_4227.tif 27 frames are being saved to U:\krios\20190717\PilA_4228.tif 27 frames are being saved to U:\krios\20190717\PilA_4229.tif 27 frames are being saved to U:\krios\20190717\PilA_4230.tif 27 frames are being saved to U:\krios\20190717\PilA_4231.tif Running script 17 at item 800 (421.52, -265.10) Remaining time to fill dewars is 21660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 140 85 40, reference by 20 152 210 20 Disparity in stage position after aligning to target position: 0.08 -0.35 (2nd move error 0.07 -0.30) Finished aligning to item in 1 rounds Trimming A by 20 150 80 30, reference by 20 140 85 40 -25.7 -3.0 unbinned pixels; 22.3 97.1 nm along two shift axes; 17.3 -99.3 nm on specimen axes Total_Shift = 51.765663 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.58 microns changed by -2.18 to target drift = 0.18 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4232.tif 27 frames are being saved to U:\krios\20190717\PilA_4233.tif 27 frames are being saved to U:\krios\20190717\PilA_4234.tif 27 frames are being saved to U:\krios\20190717\PilA_4235.tif 27 frames are being saved to U:\krios\20190717\PilA_4236.tif 27 frames are being saved to U:\krios\20190717\PilA_4237.tif Running script 17 at item 801 (419.04, -263.97) Remaining time to fill dewars is 21540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 115 20 90, reference by 22 145 20 190 Disparity in stage position after aligning to target position: 0.16 0.25 (2nd move error 0.13 0.29) Finished aligning to item in 1 rounds Trimming A by 24 105 20 85, reference by 23 115 20 90 0.4 -1.2 unbinned pixels; 5.0 0.2 nm along two shift axes; 4.6 2.0 nm on specimen axes Total_Shift = 2.58127 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.69 microns changed by -3.39 to target drift = 0.15 nm/sec Measured defocus = -1.77 microns changed by 0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4238.tif 27 frames are being saved to U:\krios\20190717\PilA_4239.tif 27 frames are being saved to U:\krios\20190717\PilA_4240.tif 27 frames are being saved to U:\krios\20190717\PilA_4241.tif 27 frames are being saved to U:\krios\20190717\PilA_4242.tif 27 frames are being saved to U:\krios\20190717\PilA_4243.tif Running script 17 at item 802 (416.53, -262.20) Remaining time to fill dewars is 21360 Dewars are NOT busy filling Trimming A by 23 145 20 195, reference by 288 21 20 245 Trimming A by 23 145 20 195, reference by 313 20 250 20 Trimming A by 23 145 20 195, reference by 22 145 20 190 Trimming A by 23 145 20 195, reference by 20 152 210 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.09 Trimming A by 21 90 20 120, reference by 22 145 20 190 Disparity in stage position after aligning to target position: 0.08 0.12 (2nd move error -0.02 0.19) Finished aligning to item in 2 rounds Trimming A by 21 85 20 115, reference by 21 90 20 120 -2.5 7.3 unbinned pixels; 30.0 1.9 nm along two shift axes; -27.8 -11.3 nm on specimen axes Total_Shift = 15.395053 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.14 microns changed by -1.94 to target drift = 0.13 nm/sec Measured defocus = -1.75 microns changed by -0.05 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4244.tif 27 frames are being saved to U:\krios\20190717\PilA_4245.tif 27 frames are being saved to U:\krios\20190717\PilA_4246.tif 27 frames are being saved to U:\krios\20190717\PilA_4247.tif 27 frames are being saved to U:\krios\20190717\PilA_4248.tif 27 frames are being saved to U:\krios\20190717\PilA_4249.tif Running script 17 at item 803 (414.01, -260.46) Remaining time to fill dewars is 21120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 66 58 20 165, reference by 22 145 20 190 Disparity in stage position after aligning to target position: 0.22 -0.01 (2nd move error 0.11 0.05) Finished aligning to item in 1 rounds Trimming A by 39 30 20 180, reference by 66 58 20 165 -0.1 0.1 unbinned pixels; 0.5 0.0 nm along two shift axes; -0.4 -0.3 nm on specimen axes Total_Shift = 0.254965 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.27 microns changed by -3.17 to target drift = 0.14 nm/sec Measured defocus = -1.94 microns changed by 0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4250.tif 27 frames are being saved to U:\krios\20190717\PilA_4251.tif 27 frames are being saved to U:\krios\20190717\PilA_4252.tif 27 frames are being saved to U:\krios\20190717\PilA_4253.tif 27 frames are being saved to U:\krios\20190717\PilA_4254.tif 27 frames are being saved to U:\krios\20190717\PilA_4255.tif Running script 17 at item 804 (401.19, -172.36) Remaining time to fill dewars is 20880 Dewars are NOT busy filling Trimming A by 24 161 190 20, reference by 296 24 20 260 Trimming A by 24 161 190 20, reference by 297 24 225 20 Trimming A by 24 161 190 20, reference by 21 155 20 225 Trimming A by 24 161 190 20, reference by 24 161 190 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.10 Trimming A by 100 51 145 20, reference by 24 161 190 20 Disparity in stage position after aligning to target position: -0.04 -0.13 (2nd move error -0.06 -0.05) Finished aligning to item in 2 rounds Trimming A by 95 51 145 20, reference by 100 51 145 20 -7.0 12.8 unbinned pixels; 56.0 6.0 nm along two shift axes; -48.1 -29.9 nm on specimen axes Total_Shift = 29.104276 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.19 microns changed by -2.19 to target drift = 0.06 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4256.tif 27 frames are being saved to U:\krios\20190717\PilA_4257.tif 27 frames are being saved to U:\krios\20190717\PilA_4258.tif 27 frames are being saved to U:\krios\20190717\PilA_4259.tif 27 frames are being saved to U:\krios\20190717\PilA_4260.tif 27 frames are being saved to U:\krios\20190717\PilA_4261.tif Running script 17 at item 805 (398.42, -170.64) Remaining time to fill dewars is 20760 Dewars are NOT busy filling Trimming A by 305 24 220 20, reference by 296 24 20 260 Trimming A by 305 24 220 20, reference by 297 24 225 20 Trimming A by 305 24 220 20, reference by 21 155 20 225 Trimming A by 305 24 220 20, reference by 24 161 190 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.10 Trimming A by 115 23 105 20, reference by 297 24 225 20 Disparity in stage position after aligning to target position: 0.14 -0.25 (2nd move error 0.10 -0.18) Finished aligning to item in 2 rounds Trimming A by 115 23 105 20, reference by 115 23 105 20 -19.3 1.3 unbinned pixels; 30.0 67.4 nm along two shift axes; -1.0 -75.2 nm on specimen axes Total_Shift = 38.628212 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.33 microns changed by -3.43 to target drift = 0.12 nm/sec Measured defocus = -2.19 microns changed by 0.09 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4262.tif 27 frames are being saved to U:\krios\20190717\PilA_4263.tif 27 frames are being saved to U:\krios\20190717\PilA_4264.tif 27 frames are being saved to U:\krios\20190717\PilA_4265.tif 27 frames are being saved to U:\krios\20190717\PilA_4266.tif 27 frames are being saved to U:\krios\20190717\PilA_4267.tif Running script 17 at item 806 (396.02, -168.97) Remaining time to fill dewars is 20520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 20 80 45, reference by 297 24 225 20 Disparity in stage position after aligning to target position: 0.09 -0.30 (2nd move error 0.04 -0.23) Finished aligning to item in 1 rounds Trimming A by 140 20 75 40, reference by 140 20 80 45 -30.2 -2.6 unbinned pixels; 29.8 112.5 nm along two shift axes; 16.5 -116.8 nm on specimen axes Total_Shift = 60.557257 Trimming A by 135 23 80 40, reference by 140 20 80 45 -1.9 -2.0 unbinned pixels; 4.8 10.0 nm along two shift axes; 8.2 -7.1 nm on specimen axes Total_Shift = 5.557632 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.13 microns changed by -2.07 to target drift = 0.19 nm/sec Measured defocus = -2.24 microns changed by 0.04 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4268.tif 27 frames are being saved to U:\krios\20190717\PilA_4269.tif 27 frames are being saved to U:\krios\20190717\PilA_4270.tif 27 frames are being saved to U:\krios\20190717\PilA_4271.tif 27 frames are being saved to U:\krios\20190717\PilA_4272.tif 27 frames are being saved to U:\krios\20190717\PilA_4273.tif Running script 17 at item 807 (393.50, -167.86) Remaining time to fill dewars is 20280 Dewars are NOT busy filling Trimming A by 294 22 20 270, reference by 296 24 20 260 Trimming A by 294 22 20 270, reference by 297 24 225 20 Trimming A by 294 22 20 270, reference by 21 155 20 225 Trimming A by 294 22 20 270, reference by 24 161 190 20 Disparity in stage position after aligning to center of map frame: 0.02 -0.05 Trimming A by 175 24 20 125, reference by 296 24 20 260 Disparity in stage position after aligning to target position: 0.20 0.17 (2nd move error 0.17 0.19) Finished aligning to item in 2 rounds Trimming A by 185 20 20 115, reference by 175 24 20 125 -0.8 -1.5 unbinned pixels; 4.5 5.0 nm along two shift axes; 6.0 -2.6 nm on specimen axes Total_Shift = 3.347641 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.94 microns changed by -3.24 to target drift = 0.14 nm/sec Measured defocus = -2.32 microns changed by 0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4274.tif 27 frames are being saved to U:\krios\20190717\PilA_4275.tif 27 frames are being saved to U:\krios\20190717\PilA_4276.tif 27 frames are being saved to U:\krios\20190717\PilA_4277.tif 27 frames are being saved to U:\krios\20190717\PilA_4278.tif 27 frames are being saved to U:\krios\20190717\PilA_4279.tif Running script 17 at item 808 (391.86, -170.34) Remaining time to fill dewars is 20100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 131 24 20 155, reference by 296 24 20 260 Disparity in stage position after aligning to target position: 0.19 0.02 (2nd move error 0.17 0.04) Finished aligning to item in 1 rounds Trimming A by 118 22 20 160, reference by 131 24 20 155 -0.3 0.9 unbinned pixels; 3.7 0.4 nm along two shift axes; -3.5 -1.2 nm on specimen axes Total_Shift = 1.905034 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.57 microns changed by -1.82 to target drift = 0.13 nm/sec Measured defocus = -2.41 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4280.tif 27 frames are being saved to U:\krios\20190717\PilA_4281.tif 27 frames are being saved to U:\krios\20190717\PilA_4282.tif 27 frames are being saved to U:\krios\20190717\PilA_4283.tif 27 frames are being saved to U:\krios\20190717\PilA_4284.tif 27 frames are being saved to U:\krios\20190717\PilA_4285.tif Running script 17 at item 809 (394.52, -171.96) Remaining time to fill dewars is 19920 Dewars are NOT busy filling Trimming A by 52 152 20 230, reference by 296 24 20 260 Trimming A by 52 152 20 230, reference by 297 24 225 20 Trimming A by 52 152 20 230, reference by 21 155 20 225 Trimming A by 52 152 20 230, reference by 24 161 190 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.06 Trimming A by 61 21 20 85, reference by 21 155 20 225 Disparity in stage position after aligning to target position: -0.02 0.23 (2nd move error -0.06 0.28) Finished aligning to item in 2 rounds Trimming A by 80 20 20 75, reference by 61 21 20 85 -4.0 12.5 unbinned pixels; 51.4 4.3 nm along two shift axes; -47.9 -18.4 nm on specimen axes Total_Shift = 26.339263 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.76 microns changed by -3.26 to target drift = 0.11 nm/sec Measured defocus = -2.56 microns changed by 0.06 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4286.tif 27 frames are being saved to U:\krios\20190717\PilA_4287.tif 27 frames are being saved to U:\krios\20190717\PilA_4288.tif 27 frames are being saved to U:\krios\20190717\PilA_4289.tif 27 frames are being saved to U:\krios\20190717\PilA_4290.tif 27 frames are being saved to U:\krios\20190717\PilA_4291.tif Running script 17 at item 810 (397.01, -173.22) Remaining time to fill dewars is 19740 Dewars are NOT busy filling Trimming A by 24 161 190 20, reference by 296 24 20 260 Trimming A by 24 161 190 20, reference by 297 24 225 20 Trimming A by 24 161 190 20, reference by 21 155 20 225 Trimming A by 24 161 190 20, reference by 24 161 190 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.05 Trimming A by 45 20 45 20, reference by 24 161 190 20 Disparity in stage position after aligning to target position: 0.01 -0.35 (2nd move error -0.02 -0.32) Finished aligning to item in 2 rounds Trimming A by 40 21 45 20, reference by 45 20 45 20 -35.4 7.3 unbinned pixels; 73.1 116.4 nm along two shift axes; -21.0 -139.3 nm on specimen axes Total_Shift = 72.294993 Trimming A by 40 30 40 20, reference by 45 20 45 20 -2.1 3.0 unbinned pixels; 13.9 3.2 nm along two shift axes; -11.3 -9.0 nm on specimen axes Total_Shift = 7.394818 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.57 microns changed by -2.03 to target drift = 0.06 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4292.tif 27 frames are being saved to U:\krios\20190717\PilA_4293.tif 27 frames are being saved to U:\krios\20190717\PilA_4294.tif 27 frames are being saved to U:\krios\20190717\PilA_4295.tif 27 frames are being saved to U:\krios\20190717\PilA_4296.tif 27 frames are being saved to U:\krios\20190717\PilA_4297.tif Running script 17 at item 811 (399.54, -175.00) Remaining time to fill dewars is 19560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 90 110 20, reference by 24 161 190 20 Disparity in stage position after aligning to target position: 0.03 -0.22 (2nd move error -0.01 -0.19) Finished aligning to item in 1 rounds Trimming A by 21 76 125 20, reference by 20 90 110 20 -22.8 26.7 unbinned pixels; 127.9 41.9 nm along two shift axes; -99.0 -94.3 nm on specimen axes Total_Shift = 70.183912 Trimming A by 23 77 125 20, reference by 20 90 110 20 -3.2 17.2 unbinned pixels; 67.3 14.3 nm along two shift axes; -66.1 -16.0 nm on specimen axes Total_Shift = 34.918247 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.59 microns changed by -3.49 to target drift = 0.05 nm/sec Measured defocus = -0.92 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4298.tif 27 frames are being saved to U:\krios\20190717\PilA_4299.tif 27 frames are being saved to U:\krios\20190717\PilA_4300.tif 27 frames are being saved to U:\krios\20190717\PilA_4301.tif 27 frames are being saved to U:\krios\20190717\PilA_4302.tif 27 frames are being saved to U:\krios\20190717\PilA_4303.tif Running script 17 at item 812 (397.89, -177.55) Remaining time to fill dewars is 19380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 120 80 20, reference by 24 161 190 20 Disparity in stage position after aligning to target position: 0.11 -0.31 (2nd move error 0.08 -0.29) Finished aligning to item in 1 rounds Trimming A by 21 120 85 20, reference by 21 120 80 20 -30.8 -2.6 unbinned pixels; 30.6 115.0 nm along two shift axes; 16.8 -119.4 nm on specimen axes Total_Shift = 61.91009 Trimming A by 24 125 85 20, reference by 21 120 80 20 -0.9 -1.7 unbinned pixels; 5.3 5.7 nm along two shift axes; 7.0 -3.0 nm on specimen axes Total_Shift = 3.89824 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.41 microns changed by -3.41 to target drift = 0.11 nm/sec Measured defocus = -1.02 microns changed by 0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4304.tif 27 frames are being saved to U:\krios\20190717\PilA_4305.tif 27 frames are being saved to U:\krios\20190717\PilA_4306.tif 27 frames are being saved to U:\krios\20190717\PilA_4307.tif 27 frames are being saved to U:\krios\20190717\PilA_4308.tif 27 frames are being saved to U:\krios\20190717\PilA_4309.tif Running script 17 at item 813 (395.44, -175.79) Remaining time to fill dewars is 19080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 65 20 60, reference by 24 161 190 20 Disparity in stage position after aligning to target position: 0.01 -0.44 (2nd move error -0.02 -0.41) Finished aligning to item in 1 rounds Trimming A by 21 61 20 75, reference by 20 65 20 60 -34.1 9.3 unbinned pixels; 78.5 108.7 nm along two shift axes; -28.8 -134.5 nm on specimen axes Total_Shift = 70.591086 Trimming A by 20 75 20 65, reference by 20 65 20 60 2.1 4.6 unbinned pixels; 14.2 14.4 nm along two shift axes; -18.3 7.1 nm on specimen axes Total_Shift = 10.091673 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.20 microns changed by -3.30 to target drift = 0.03 nm/sec Measured defocus = -1.12 microns changed by 0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4310.tif 27 frames are being saved to U:\krios\20190717\PilA_4311.tif 27 frames are being saved to U:\krios\20190717\PilA_4312.tif 27 frames are being saved to U:\krios\20190717\PilA_4313.tif 27 frames are being saved to U:\krios\20190717\PilA_4314.tif 27 frames are being saved to U:\krios\20190717\PilA_4315.tif Running script 17 at item 814 (393.00, -174.69) Remaining time to fill dewars is 18900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 42 20 110, reference by 21 155 20 225 Disparity in stage position after aligning to target position: 0.02 0.09 (2nd move error -0.03 0.13) Finished aligning to item in 1 rounds Trimming A by 36 55 20 95, reference by 21 42 20 110 0.7 6.6 unbinned pixels; 23.4 12.5 nm along two shift axes; -25.9 1.3 nm on specimen axes Total_Shift = 13.321009 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.99 microns changed by -3.19 to target drift = 0.16 nm/sec Measured defocus = -1.16 microns changed by -0.04 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4316.tif 27 frames are being saved to U:\krios\20190717\PilA_4317.tif 27 frames are being saved to U:\krios\20190717\PilA_4318.tif 27 frames are being saved to U:\krios\20190717\PilA_4319.tif 27 frames are being saved to U:\krios\20190717\PilA_4320.tif 27 frames are being saved to U:\krios\20190717\PilA_4321.tif Running script 17 at item 815 (390.48, -172.96) Remaining time to fill dewars is 18660 Dewars are NOT busy filling Trimming A by 23 160 20 225, reference by 296 24 20 260 Trimming A by 23 160 20 225, reference by 297 24 225 20 Trimming A by 23 160 20 225, reference by 21 155 20 225 Trimming A by 23 160 20 225, reference by 24 161 190 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.09 Trimming A by 72 22 20 185, reference by 21 155 20 225 Disparity in stage position after aligning to target position: -0.02 -0.00 (2nd move error -0.09 0.06) Finished aligning to item in 2 rounds Trimming A by 76 20 20 185, reference by 72 22 20 185 1.1 18.3 unbinned pixels; 65.8 31.3 nm along two shift axes; -71.3 0.3 nm on specimen axes Total_Shift = 36.625503 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.78 microns changed by -3.08 to target drift = 0.15 nm/sec Measured defocus = -1.28 microns changed by -0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4322.tif 27 frames are being saved to U:\krios\20190717\PilA_4323.tif 27 frames are being saved to U:\krios\20190717\PilA_4324.tif 27 frames are being saved to U:\krios\20190717\PilA_4325.tif 27 frames are being saved to U:\krios\20190717\PilA_4326.tif 27 frames are being saved to U:\krios\20190717\PilA_4327.tif Running script 17 at item 816 (372.20, -743.65) Remaining time to fill dewars is 18480 Dewars are NOT busy filling Trimming A by 140 21 170 20, reference by 147 22 20 245 Trimming A by 140 21 170 20, reference by 135 21 170 20 Trimming A by 140 21 170 20, reference by 24 330 20 285 Trimming A by 140 21 170 20, reference by 20 335 220 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.05 Trimming A by 23 97 155 20, reference by 135 21 170 20 Disparity in stage position after aligning to target position: -0.03 -0.04 (2nd move error 0.05 -0.02) Finished aligning to item in 2 rounds Trimming A by 21 110 145 20, reference by 23 97 155 20 -3.5 -3.2 unbinned pixels; 7.2 17.4 nm along two shift axes; 13.2 -12.9 nm on specimen axes Total_Shift = 9.483562 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.27 microns changed by -3.67 to target drift = 0.09 nm/sec Measured defocus = -1.49 microns changed by 0.10 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4328.tif 27 frames are being saved to U:\krios\20190717\PilA_4329.tif 27 frames are being saved to U:\krios\20190717\PilA_4330.tif 27 frames are being saved to U:\krios\20190717\PilA_4331.tif 27 frames are being saved to U:\krios\20190717\PilA_4332.tif 27 frames are being saved to U:\krios\20190717\PilA_4333.tif Running script 17 at item 817 (369.70, -741.91) Remaining time to fill dewars is 18300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 50 46 70 20, reference by 135 21 170 20 Disparity in stage position after aligning to target position: -0.06 -0.23 (2nd move error 0.02 -0.20) Finished aligning to item in 1 rounds Trimming A by 36 36 80 20, reference by 50 46 70 20 -24.1 -0.8 unbinned pixels; 28.4 88.1 nm along two shift axes; 8.3 -93.6 nm on specimen axes Total_Shift = 48.24504 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.61 microns changed by -2.11 to target drift = 0.11 nm/sec Measured defocus = -1.55 microns changed by 0.05 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4334.tif 27 frames are being saved to U:\krios\20190717\PilA_4335.tif 27 frames are being saved to U:\krios\20190717\PilA_4336.tif 27 frames are being saved to U:\krios\20190717\PilA_4337.tif 27 frames are being saved to U:\krios\20190717\PilA_4338.tif 27 frames are being saved to U:\krios\20190717\PilA_4339.tif Running script 17 at item 818 (371.42, -739.37) Remaining time to fill dewars is 18060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 120 21 140 20, reference by 135 21 170 20 Disparity in stage position after aligning to target position: -0.09 -0.15 (2nd move error -0.01 -0.12) Finished aligning to item in 1 rounds Trimming A by 125 20 135 20, reference by 120 21 140 20 -16.6 4.8 unbinned pixels; 39.3 52.7 nm along two shift axes; -15.1 -65.7 nm on specimen axes Total_Shift = 34.600219 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.78 microns changed by -3.38 to target drift = 0.03 nm/sec Measured defocus = -1.67 microns changed by 0.07 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4340.tif 27 frames are being saved to U:\krios\20190717\PilA_4341.tif 27 frames are being saved to U:\krios\20190717\PilA_4342.tif 27 frames are being saved to U:\krios\20190717\PilA_4343.tif 27 frames are being saved to U:\krios\20190717\PilA_4344.tif 27 frames are being saved to U:\krios\20190717\PilA_4345.tif Running script 17 at item 819 (368.95, -737.55) Remaining time to fill dewars is 17940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 155 23 100 20, reference by 135 21 170 20 Disparity in stage position after aligning to target position: -0.09 -0.27 (2nd move error -0.02 -0.23) Finished aligning to item in 1 rounds Trimming A by 150 20 100 20, reference by 155 23 100 20 -33.4 16.1 unbinned pixels; 103.0 96.1 nm along two shift axes; -55.8 -133.5 nm on specimen axes Total_Shift = 74.276487 Trimming A by 150 23 100 20, reference by 155 23 100 20 -9.1 8.0 unbinned pixels; 41.5 20.8 nm along two shift axes; -29.4 -37.2 nm on specimen axes Total_Shift = 24.348234 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.03 microns changed by -1.73 to target drift = 0.01 nm/sec Measured defocus = -1.65 microns changed by -0.05 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4346.tif 27 frames are being saved to U:\krios\20190717\PilA_4347.tif 27 frames are being saved to U:\krios\20190717\PilA_4348.tif 27 frames are being saved to U:\krios\20190717\PilA_4349.tif 27 frames are being saved to U:\krios\20190717\PilA_4350.tif 27 frames are being saved to U:\krios\20190717\PilA_4351.tif Running script 17 at item 820 (367.24, -740.20) Remaining time to fill dewars is 17700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 85 20 25 65, reference by 135 21 170 20 Disparity in stage position after aligning to target position: -0.05 -0.45 (2nd move error 0.03 -0.42) Finished aligning to item in 1 rounds Trimming A by 70 20 30 70, reference by 85 20 25 65 -32.1 -3.3 unbinned pixels; 29.7 120.7 nm along two shift axes; 19.7 -124.3 nm on specimen axes Total_Shift = 64.621369 Trimming A by 75 20 20 60, reference by 85 20 25 65 -1.4 -0.3 unbinned pixels; 0.6 5.6 nm along two shift axes; 1.7 -5.4 nm on specimen axes Total_Shift = 2.92107 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.27 microns changed by -3.07 to target drift = 0.14 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4352.tif 27 frames are being saved to U:\krios\20190717\PilA_4353.tif 27 frames are being saved to U:\krios\20190717\PilA_4354.tif 27 frames are being saved to U:\krios\20190717\PilA_4355.tif 27 frames are being saved to U:\krios\20190717\PilA_4356.tif 27 frames are being saved to U:\krios\20190717\PilA_4357.tif Running script 17 at item 821 (364.64, -739.24) Remaining time to fill dewars is 17520 Dewars are NOT busy filling Trimming A by 152 21 20 245, reference by 147 22 20 245 Trimming A by 152 21 20 245, reference by 135 21 170 20 Trimming A by 152 21 20 245, reference by 24 330 20 285 Trimming A by 152 21 20 245, reference by 20 335 220 20 Disparity in stage position after aligning to center of map frame: -0.00 -0.00 Trimming A by 130 20 20 130, reference by 147 22 20 245 Disparity in stage position after aligning to target position: 0.13 0.34 (2nd move error 0.13 0.32) Finished aligning to item in 2 rounds Trimming A by 140 23 20 125, reference by 130 20 20 130 5.8 2.9 unbinned pixels; 3.2 25.2 nm along two shift axes; -12.5 21.8 nm on specimen axes Total_Shift = 12.929918 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.88 microns changed by -2.78 to target drift = 0.14 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4358.tif 27 frames are being saved to U:\krios\20190717\PilA_4359.tif 27 frames are being saved to U:\krios\20190717\PilA_4360.tif 27 frames are being saved to U:\krios\20190717\PilA_4361.tif 27 frames are being saved to U:\krios\20190717\PilA_4362.tif 27 frames are being saved to U:\krios\20190717\PilA_4363.tif Running script 17 at item 822 (363.04, -741.74) Remaining time to fill dewars is 17280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 77 22 20 165, reference by 147 22 20 245 Disparity in stage position after aligning to target position: 0.23 0.15 (2nd move error 0.22 0.13) Finished aligning to item in 1 rounds Trimming A by 86 24 20 155, reference by 77 22 20 165 2.6 -3.7 unbinned pixels; 17.1 3.6 nm along two shift axes; 14.0 10.7 nm on specimen axes Total_Shift = 9.07943 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.76 microns changed by -2.76 to target drift = 0.15 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4364.tif 27 frames are being saved to U:\krios\20190717\PilA_4365.tif 27 frames are being saved to U:\krios\20190717\PilA_4366.tif 27 frames are being saved to U:\krios\20190717\PilA_4367.tif 27 frames are being saved to U:\krios\20190717\PilA_4368.tif 27 frames are being saved to U:\krios\20190717\PilA_4369.tif Running script 17 at item 823 (365.34, -743.51) Remaining time to fill dewars is 17100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 70 20 100, reference by 147 22 20 245 Disparity in stage position after aligning to target position: 0.40 0.41 (2nd move error 0.40 0.39) Finished aligning to item in 1 rounds Trimming A by 22 42 20 105, reference by 21 70 20 100 9.8 0.8 unbinned pixels; 9.9 36.5 nm along two shift axes; -5.1 38.0 nm on specimen axes Total_Shift = 19.680524 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.94 microns changed by -3.04 to target drift = 0.13 nm/sec Measured defocus = -2.10 microns changed by 0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4370.tif 27 frames are being saved to U:\krios\20190717\PilA_4371.tif 27 frames are being saved to U:\krios\20190717\PilA_4372.tif 27 frames are being saved to U:\krios\20190717\PilA_4373.tif 27 frames are being saved to U:\krios\20190717\PilA_4374.tif 27 frames are being saved to U:\krios\20190717\PilA_4375.tif Running script 17 at item 824 (368.13, -744.40) Remaining time to fill dewars is 16800 Dewars are NOT busy filling Trimming A by 140 21 170 20, reference by 147 22 20 245 Trimming A by 140 21 170 20, reference by 135 21 170 20 Trimming A by 140 21 170 20, reference by 24 330 20 285 Trimming A by 140 21 170 20, reference by 20 335 220 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.03 Trimming A by 20 75 50 60, reference by 135 21 170 20 Disparity in stage position after aligning to target position: -0.03 -0.41 (2nd move error 0.05 -0.40) Finished aligning to item in 2 rounds Trimming A by 21 85 50 55, reference by 20 75 50 60 -35.6 -2.2 unbinned pixels; 38.4 131.3 nm along two shift axes; 16.0 -137.9 nm on specimen axes Total_Shift = 71.251853 Trimming A by 21 85 50 65, reference by 20 75 50 60 -1.1 -5.2 unbinned pixels; 17.8 11.7 nm along two shift axes; 20.5 -3.1 nm on specimen axes Total_Shift = 10.633566 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.17 microns changed by -2.03 to target drift = 0.21 nm/sec Measured defocus = -2.27 microns changed by 0.07 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4376.tif 27 frames are being saved to U:\krios\20190717\PilA_4377.tif 27 frames are being saved to U:\krios\20190717\PilA_4378.tif 27 frames are being saved to U:\krios\20190717\PilA_4379.tif 27 frames are being saved to U:\krios\20190717\PilA_4380.tif 27 frames are being saved to U:\krios\20190717\PilA_4381.tif Running script 17 at item 825 (363.98, -745.96) Remaining time to fill dewars is 16560 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 20 140 20 155, reference by 147 22 20 245 Disparity in stage position after aligning to target position: 0.77 -0.98 (2nd move error 0.77 -1.00) Finished aligning to item in 1 rounds Trimming A by 21 155 50 145, reference by 20 140 20 155 -34.9 5.0 unbinned pixels; 63.9 118.2 nm along two shift axes; -12.0 -136.8 nm on specimen axes Total_Shift = 70.515461 Trimming A by 24 155 20 155, reference by 20 140 20 155 0.2 -3.5 unbinned pixels; 13.2 4.7 nm along two shift axes; 13.7 1.4 nm on specimen axes Total_Shift = 7.049693 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.85 microns changed by -3.15 to target drift = 0.08 nm/sec Measured defocus = -2.35 microns changed by 0.05 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4382.tif 27 frames are being saved to U:\krios\20190717\PilA_4383.tif 27 frames are being saved to U:\krios\20190717\PilA_4384.tif 27 frames are being saved to U:\krios\20190717\PilA_4385.tif 27 frames are being saved to U:\krios\20190717\PilA_4386.tif 27 frames are being saved to U:\krios\20190717\PilA_4387.tif Running script 17 at item 826 (-766.31, -381.95) Remaining time to fill dewars is 16320 Dewars are NOT busy filling Trimming A by 278 21 190 20, reference by 290 23 20 290 Trimming A by 278 21 190 20, reference by 275 21 180 20 Trimming A by 278 21 190 20, reference by 24 210 20 280 Trimming A by 278 21 190 20, reference by 23 195 160 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.16 Trimming A by 165 21 150 20, reference by 275 21 180 20 Disparity in stage position after aligning to target position: 0.03 -0.28 (2nd move error 0.07 -0.13) Finished aligning to item in 2 rounds Trimming A by 152 20 160 20, reference by 165 21 150 20 -12.5 0.5 unbinned pixels; 18.2 44.1 nm along two shift axes; 0.6 -48.6 nm on specimen axes Total_Shift = 24.937491 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.38 microns changed by -2.02 to target drift = 0.26 nm/sec Measured defocus = -2.45 microns changed by 0.05 to target drift = 0.25 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4388.tif 27 frames are being saved to U:\krios\20190717\PilA_4389.tif 27 frames are being saved to U:\krios\20190717\PilA_4390.tif 27 frames are being saved to U:\krios\20190717\PilA_4391.tif 27 frames are being saved to U:\krios\20190717\PilA_4392.tif 27 frames are being saved to U:\krios\20190717\PilA_4393.tif Running script 17 at item 827 (-772.92, -381.52) Remaining time to fill dewars is 16140 Dewars are NOT busy filling Trimming A by 285 20 20 295, reference by 290 23 20 290 Trimming A by 285 20 20 295, reference by 275 21 180 20 Trimming A by 285 20 20 295, reference by 24 210 20 280 Trimming A by 285 20 20 295, reference by 23 195 160 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.05 Trimming A by 170 22 20 145, reference by 290 23 20 290 Disparity in stage position after aligning to target position: 0.07 0.16 (2nd move error 0.11 0.18) Finished aligning to item in 2 rounds Trimming A by 175 20 20 140, reference by 170 22 20 145 4.4 0.4 unbinned pixels; 4.3 16.3 nm along two shift axes; -2.4 16.9 nm on specimen axes Total_Shift = 8.775351 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.08 microns changed by -3.58 to target drift = 0.17 nm/sec Measured defocus = -2.54 microns changed by 0.04 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4394.tif 27 frames are being saved to U:\krios\20190717\PilA_4395.tif 27 frames are being saved to U:\krios\20190717\PilA_4396.tif 27 frames are being saved to U:\krios\20190717\PilA_4397.tif 27 frames are being saved to U:\krios\20190717\PilA_4398.tif 27 frames are being saved to U:\krios\20190717\PilA_4399.tif Running script 17 at item 828 (-770.41, -382.74) Remaining time to fill dewars is 15960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 120 20 45 75, reference by 275 21 180 20 Disparity in stage position after aligning to target position: -0.07 -0.27 (2nd move error -0.03 -0.13) Finished aligning to item in 1 rounds Trimming A by 120 20 40 70, reference by 120 20 45 75 -18.6 8.1 unbinned pixels; 54.0 54.9 nm along two shift axes; -27.5 -74.2 nm on specimen axes Total_Shift = 40.609091 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.41 microns changed by -2.19 to target drift = 0.05 nm/sec Measured defocus = -2.63 microns changed by 0.03 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4400.tif 27 frames are being saved to U:\krios\20190717\PilA_4401.tif 27 frames are being saved to U:\krios\20190717\PilA_4402.tif 27 frames are being saved to U:\krios\20190717\PilA_4403.tif 27 frames are being saved to U:\krios\20190717\PilA_4404.tif 27 frames are being saved to U:\krios\20190717\PilA_4405.tif Running script 17 at item 829 (-767.98, -384.45) Remaining time to fill dewars is 15780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 20 85 20, reference by 275 21 180 20 Disparity in stage position after aligning to target position: -0.07 -0.22 (2nd move error -0.02 -0.09) Finished aligning to item in 1 rounds Trimming A by 90 20 85 20, reference by 90 20 85 20 -11.7 6.6 unbinned pixels; 39.4 32.1 nm along two shift axes; -23.1 -46.7 nm on specimen axes Total_Shift = 26.764707 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.64 microns changed by -3.54 to target drift = 0.05 nm/sec Measured defocus = -0.96 microns changed by 0.06 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4406.tif 27 frames are being saved to U:\krios\20190717\PilA_4407.tif 27 frames are being saved to U:\krios\20190717\PilA_4408.tif 27 frames are being saved to U:\krios\20190717\PilA_4409.tif 27 frames are being saved to U:\krios\20190717\PilA_4410.tif 27 frames are being saved to U:\krios\20190717\PilA_4411.tif Running script 17 at item 830 (-765.37, -386.17) Remaining time to fill dewars is 15600 Dewars are NOT busy filling Trimming A by 21 190 160 20, reference by 290 23 20 290 Trimming A by 21 190 160 20, reference by 275 21 180 20 Trimming A by 21 190 160 20, reference by 24 210 20 280 Trimming A by 21 190 160 20, reference by 23 195 160 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.11 Trimming A by 56 56 140 20, reference by 23 195 160 20 Disparity in stage position after aligning to target position: -0.15 -0.11 (2nd move error -0.08 -0.03) Finished aligning to item in 2 rounds Trimming A by 56 51 140 20, reference by 56 56 140 20 -3.8 16.7 unbinned pixels; 66.4 11.3 nm along two shift axes; -64.1 -18.4 nm on specimen axes Total_Shift = 34.246671 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.42 microns changed by -3.42 to target drift = 0.09 nm/sec Measured defocus = -1.04 microns changed by 0.04 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4412.tif 27 frames are being saved to U:\krios\20190717\PilA_4413.tif 27 frames are being saved to U:\krios\20190717\PilA_4414.tif 27 frames are being saved to U:\krios\20190717\PilA_4415.tif 27 frames are being saved to U:\krios\20190717\PilA_4416.tif 27 frames are being saved to U:\krios\20190717\PilA_4417.tif Running script 17 at item 831 (-766.97, -388.71) Remaining time to fill dewars is 15360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 100 95 20, reference by 23 195 160 20 Disparity in stage position after aligning to target position: -0.06 -0.21 (2nd move error 0.02 -0.12) Finished aligning to item in 1 rounds Trimming A by 23 105 95 20, reference by 24 100 95 20 -15.6 -1.3 unbinned pixels; 15.6 58.3 nm along two shift axes; 8.4 -60.6 nm on specimen axes Total_Shift = 31.39983 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.51 microns changed by -3.61 to target drift = 0.06 nm/sec Measured defocus = -1.14 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4418.tif 27 frames are being saved to U:\krios\20190717\PilA_4419.tif 27 frames are being saved to U:\krios\20190717\PilA_4420.tif 27 frames are being saved to U:\krios\20190717\PilA_4421.tif 27 frames are being saved to U:\krios\20190717\PilA_4422.tif 27 frames are being saved to U:\krios\20190717\PilA_4423.tif Running script 17 at item 832 (-769.46, -386.98) Remaining time to fill dewars is 15120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 25 45 20 45, reference by 23 195 160 20 Disparity in stage position after aligning to target position: -0.16 -0.26 (2nd move error -0.09 -0.18) Finished aligning to item in 1 rounds Trimming A by 25 45 20 45, reference by 25 45 20 45 -21.6 17.8 unbinned pixels; 93.6 51.2 nm along two shift axes; -64.6 -87.9 nm on specimen axes Total_Shift = 56.015748 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.54 microns changed by -3.74 to target drift = 0.06 nm/sec Measured defocus = -1.26 microns changed by 0.06 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4424.tif 27 frames are being saved to U:\krios\20190717\PilA_4425.tif 27 frames are being saved to U:\krios\20190717\PilA_4426.tif 27 frames are being saved to U:\krios\20190717\PilA_4427.tif 27 frames are being saved to U:\krios\20190717\PilA_4428.tif 27 frames are being saved to U:\krios\20190717\PilA_4429.tif Running script 17 at item 833 (-771.93, -385.75) Remaining time to fill dewars is 14940 Dewars are NOT busy filling Trimming A by 21 205 20 280, reference by 290 23 20 290 Trimming A by 21 205 20 280, reference by 275 21 180 20 Trimming A by 21 205 20 280, reference by 24 210 20 280 Trimming A by 21 205 20 280, reference by 23 195 160 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.09 Trimming A by 47 24 20 110, reference by 24 210 20 280 Disparity in stage position after aligning to target position: -0.07 0.20 (2nd move error 0.00 0.27) Finished aligning to item in 2 rounds Trimming A by 52 22 20 110, reference by 47 24 20 110 0.9 2.3 unbinned pixels; 7.5 6.6 nm along two shift axes; -9.3 2.9 nm on specimen axes Total_Shift = 4.974892 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.47 microns changed by -3.77 to target drift = 0.16 nm/sec Measured defocus = -1.38 microns changed by 0.08 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4430.tif 27 frames are being saved to U:\krios\20190717\PilA_4431.tif 27 frames are being saved to U:\krios\20190717\PilA_4432.tif 27 frames are being saved to U:\krios\20190717\PilA_4433.tif 27 frames are being saved to U:\krios\20190717\PilA_4434.tif 27 frames are being saved to U:\krios\20190717\PilA_4435.tif Running script 17 at item 834 (-773.54, -388.42) Remaining time to fill dewars is 14760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 105 20 145, reference by 24 210 20 280 Disparity in stage position after aligning to target position: -0.06 0.03 (2nd move error 0.02 0.10) Finished aligning to item in 1 rounds Trimming A by 21 100 20 145, reference by 21 105 20 145 -1.8 0.6 unbinned pixels; 4.5 5.5 nm along two shift axes; -1.9 -7.0 nm on specimen axes Total_Shift = 3.726307 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.16 microns changed by -2.56 to target drift = 0.11 nm/sec Measured defocus = -1.45 microns changed by 0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4436.tif 27 frames are being saved to U:\krios\20190717\PilA_4437.tif 27 frames are being saved to U:\krios\20190717\PilA_4438.tif 27 frames are being saved to U:\krios\20190717\PilA_4439.tif 27 frames are being saved to U:\krios\20190717\PilA_4440.tif 27 frames are being saved to U:\krios\20190717\PilA_4441.tif Running script 17 at item 835 (-771.10, -389.59) Remaining time to fill dewars is 14580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 102 20 120, reference by 23 195 160 20 Disparity in stage position after aligning to target position: -0.08 -0.35 (2nd move error -0.01 -0.26) Finished aligning to item in 1 rounds Trimming A by 24 120 20 110, reference by 22 102 20 120 -26.5 5.1 unbinned pixels; 53.5 87.6 nm along two shift axes; -14.4 -104.1 nm on specimen axes Total_Shift = 53.975098 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.41 microns changed by -3.91 to target drift = 0.05 nm/sec Measured defocus = -1.57 microns changed by 0.08 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4442.tif 27 frames are being saved to U:\krios\20190717\PilA_4443.tif 27 frames are being saved to U:\krios\20190717\PilA_4444.tif 27 frames are being saved to U:\krios\20190717\PilA_4445.tif 27 frames are being saved to U:\krios\20190717\PilA_4446.tif 27 frames are being saved to U:\krios\20190717\PilA_4447.tif Running script 17 at item 836 (-528.46, -76.58) Remaining time to fill dewars is 14400 Dewars are NOT busy filling Trimming A by 234 23 170 20, reference by 207 22 20 230 Trimming A by 234 23 170 20, reference by 229 24 160 20 Trimming A by 234 23 170 20, reference by 22 145 20 185 Trimming A by 234 23 170 20, reference by 21 140 135 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.08 Trimming A by 160 21 165 20, reference by 229 24 160 20 Disparity in stage position after aligning to target position: 0.68 -0.15 (2nd move error 0.95 -0.09) Finished aligning to item in 2 rounds Trimming A by 165 22 180 20, reference by 160 21 165 20 -1.3 -18.2 unbinned pixels; 65.2 32.2 nm along two shift axes; 71.1 -1.3 nm on specimen axes Total_Shift = 36.511896 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.39 microns changed by -1.99 to target drift = 0.27 nm/sec Measured defocus = -1.57 microns changed by -0.03 to target drift = 0.17 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4448.tif 27 frames are being saved to U:\krios\20190717\PilA_4449.tif 27 frames are being saved to U:\krios\20190717\PilA_4450.tif 27 frames are being saved to U:\krios\20190717\PilA_4451.tif 27 frames are being saved to U:\krios\20190717\PilA_4452.tif 27 frames are being saved to U:\krios\20190717\PilA_4453.tif Running script 17 at item 837 (-530.99, -74.92) Remaining time to fill dewars is 14220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 175 23 130 20, reference by 229 24 160 20 Disparity in stage position after aligning to target position: -0.19 -0.14 (2nd move error 0.09 -0.08) Finished aligning to item in 1 rounds Trimming A by 175 23 130 20, reference by 175 23 130 20 -9.3 -0.0 unbinned pixels; 12.0 33.4 nm along two shift axes; 2.1 -36.0 nm on specimen axes Total_Shift = 18.511293 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.74 microns changed by -3.44 to target drift = 0.08 nm/sec Measured defocus = -1.71 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4454.tif 27 frames are being saved to U:\krios\20190717\PilA_4455.tif 27 frames are being saved to U:\krios\20190717\PilA_4456.tif 27 frames are being saved to U:\krios\20190717\PilA_4457.tif 27 frames are being saved to U:\krios\20190717\PilA_4458.tif 27 frames are being saved to U:\krios\20190717\PilA_4459.tif Running script 17 at item 838 (-537.74, -74.43) Remaining time to fill dewars is 13980 Dewars are NOT busy filling Trimming A by 220 22 20 215, reference by 207 22 20 230 Trimming A by 220 22 20 215, reference by 229 24 160 20 Trimming A by 220 22 20 215, reference by 22 145 20 185 Trimming A by 220 22 20 215, reference by 21 140 135 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.04 Trimming A by 140 20 20 90, reference by 207 22 20 230 Disparity in stage position after aligning to target position: -0.01 0.11 (2nd move error 0.19 0.13) Finished aligning to item in 2 rounds Trimming A by 140 20 20 85, reference by 140 20 20 90 1.4 5.9 unbinned pixels; 19.8 14.0 nm along two shift axes; -23.2 4.3 nm on specimen axes Total_Shift = 12.112543 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.63 microns changed by -2.43 to target drift = 0.10 nm/sec Measured defocus = -1.85 microns changed by 0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4460.tif 27 frames are being saved to U:\krios\20190717\PilA_4461.tif 27 frames are being saved to U:\krios\20190717\PilA_4462.tif 27 frames are being saved to U:\krios\20190717\PilA_4463.tif 27 frames are being saved to U:\krios\20190717\PilA_4464.tif 27 frames are being saved to U:\krios\20190717\PilA_4465.tif Running script 17 at item 839 (-532.52, -77.56) Remaining time to fill dewars is 13740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 23 75 20, reference by 229 24 160 20 Disparity in stage position after aligning to target position: -0.22 -0.10 (2nd move error 0.06 -0.04) Finished aligning to item in 1 rounds Trimming A by 90 23 75 20, reference by 90 23 75 20 -4.6 0.1 unbinned pixels; 6.4 16.6 nm along two shift axes; 0.6 -18.1 nm on specimen axes Total_Shift = 9.295729 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.53 microns changed by -3.42 to target drift = 0.07 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4466.tif 27 frames are being saved to U:\krios\20190717\PilA_4467.tif 27 frames are being saved to U:\krios\20190717\PilA_4468.tif 27 frames are being saved to U:\krios\20190717\PilA_4469.tif 27 frames are being saved to U:\krios\20190717\PilA_4470.tif 27 frames are being saved to U:\krios\20190717\PilA_4471.tif Running script 17 at item 840 (-530.08, -79.25) Remaining time to fill dewars is 13500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 85 24 90 20, reference by 229 24 160 20 Disparity in stage position after aligning to target position: -0.13 -0.10 (2nd move error 0.14 -0.04) Finished aligning to item in 1 rounds Trimming A by 85 24 90 20, reference by 85 24 90 20 -2.8 1.5 unbinned pixels; 9.2 7.6 nm along two shift axes; -5.4 -11.0 nm on specimen axes Total_Shift = 6.300891 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = -0.02 microns changed by -1.98 to target drift = 0.11 nm/sec Measured defocus = -1.96 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4472.tif 27 frames are being saved to U:\krios\20190717\PilA_4473.tif 27 frames are being saved to U:\krios\20190717\PilA_4474.tif 27 frames are being saved to U:\krios\20190717\PilA_4475.tif 27 frames are being saved to U:\krios\20190717\PilA_4476.tif 27 frames are being saved to U:\krios\20190717\PilA_4477.tif Running script 17 at item 841 (-529.01, -83.44) Remaining time to fill dewars is 13320 Dewars are NOT busy filling Trimming A by 20 145 140 20, reference by 207 22 20 230 Trimming A by 20 145 140 20, reference by 229 24 160 20 Trimming A by 20 145 140 20, reference by 22 145 20 185 Trimming A by 20 145 140 20, reference by 21 140 135 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.09 Trimming A by 20 70 145 20, reference by 21 140 135 20 Disparity in stage position after aligning to target position: -0.26 -0.09 (2nd move error -0.04 -0.03) Finished aligning to item in 2 rounds Trimming A by 23 78 140 20, reference by 20 70 145 20 -5.4 9.0 unbinned pixels; 39.9 5.8 nm along two shift axes; -33.7 -22.7 nm on specimen axes Total_Shift = 20.881943 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.25 microns changed by -3.35 to target drift = 0.07 nm/sec Measured defocus = -2.21 microns changed by 0.11 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4478.tif 27 frames are being saved to U:\krios\20190717\PilA_4479.tif 27 frames are being saved to U:\krios\20190717\PilA_4480.tif 27 frames are being saved to U:\krios\20190717\PilA_4481.tif 27 frames are being saved to U:\krios\20190717\PilA_4482.tif 27 frames are being saved to U:\krios\20190717\PilA_4483.tif Running script 17 at item 842 (-531.50, -81.72) Remaining time to fill dewars is 13140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 25 60 20, reference by 21 140 135 20 Disparity in stage position after aligning to target position: -0.21 -0.16 (2nd move error 0.00 -0.09) Finished aligning to item in 1 rounds Trimming A by 21 21 65 20, reference by 24 25 60 20 -13.3 1.5 unbinned pixels; 23.0 45.7 nm along two shift axes; -3.1 -52.2 nm on specimen axes Total_Shift = 26.851675 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.17 microns changed by -2.03 to target drift = 0.06 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4484.tif 27 frames are being saved to U:\krios\20190717\PilA_4485.tif 27 frames are being saved to U:\krios\20190717\PilA_4486.tif 27 frames are being saved to U:\krios\20190717\PilA_4487.tif 27 frames are being saved to U:\krios\20190717\PilA_4488.tif 27 frames are being saved to U:\krios\20190717\PilA_4489.tif Running script 17 at item 843 (-534.04, -79.94) Remaining time to fill dewars is 12840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 21 140 135 20 Disparity in stage position after aligning to target position: -0.33 -0.27 (2nd move error -0.11 -0.18) Finished aligning to item in 1 rounds -22.2 20.4 unbinned pixels; 103.9 49.4 nm along two shift axes; -74.5 -90.8 nm on specimen axes Total_Shift = 60.287266 -3.7 13.1 unbinned pixels; 53.0 6.2 nm along two shift axes; -50.1 -17.3 nm on specimen axes Total_Shift = 27.212764 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.18 microns changed by -3.48 to target drift = 0.04 nm/sec Measured defocus = -2.34 microns changed by 0.04 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4490.tif 27 frames are being saved to U:\krios\20190717\PilA_4491.tif 27 frames are being saved to U:\krios\20190717\PilA_4492.tif 27 frames are being saved to U:\krios\20190717\PilA_4493.tif 27 frames are being saved to U:\krios\20190717\PilA_4494.tif 27 frames are being saved to U:\krios\20190717\PilA_4495.tif Running script 17 at item 844 (-536.63, -78.57) Remaining time to fill dewars is 12660 Dewars are NOT busy filling Trimming A by 204 22 20 230, reference by 207 22 20 230 Trimming A by 204 22 20 230, reference by 229 24 160 20 Trimming A by 204 22 20 230, reference by 22 145 20 185 Trimming A by 204 22 20 230, reference by 21 140 135 20 Disparity in stage position after aligning to center of map frame: -0.15 -0.03 Trimming A by 40 20 20 40, reference by 207 22 20 230 Disparity in stage position after aligning to target position: 0.05 0.19 (2nd move error 0.18 0.20) Finished aligning to item in 2 rounds Trimming A by 40 20 20 40, reference by 40 20 20 40 -0.2 3.5 unbinned pixels; 12.9 4.6 nm along two shift axes; -13.4 -1.3 nm on specimen axes Total_Shift = 6.917141 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.22 microns changed by -2.18 to target drift = 0.10 nm/sec Measured defocus = -2.35 microns changed by -0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4496.tif 27 frames are being saved to U:\krios\20190717\PilA_4497.tif 27 frames are being saved to U:\krios\20190717\PilA_4498.tif 27 frames are being saved to U:\krios\20190717\PilA_4499.tif 27 frames are being saved to U:\krios\20190717\PilA_4500.tif 27 frames are being saved to U:\krios\20190717\PilA_4501.tif Running script 17 at item 845 (-539.36, -77.04) Remaining time to fill dewars is 12480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 110 20 20 110, reference by 207 22 20 230 Disparity in stage position after aligning to target position: 0.00 0.02 (2nd move error 0.14 0.03) Finished aligning to item in 1 rounds Trimming A by 110 24 20 105, reference by 110 20 20 110 0.5 -5.3 unbinned pixels; 20.1 6.3 nm along two shift axes; 20.5 2.9 nm on specimen axes Total_Shift = 10.65217 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.10 microns changed by -3.60 to target drift = 0.11 nm/sec Measured defocus = -2.54 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4502.tif 27 frames are being saved to U:\krios\20190717\PilA_4503.tif 27 frames are being saved to U:\krios\20190717\PilA_4504.tif 27 frames are being saved to U:\krios\20190717\PilA_4505.tif 27 frames are being saved to U:\krios\20190717\PilA_4506.tif 27 frames are being saved to U:\krios\20190717\PilA_4507.tif Running script 17 at item 846 (-540.87, -79.52) Remaining time to fill dewars is 12240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 63 23 20 140, reference by 207 22 20 230 Disparity in stage position after aligning to target position: 0.07 -0.02 (2nd move error 0.20 -0.01) Finished aligning to item in 1 rounds Trimming A by 63 23 20 135, reference by 63 23 20 140 -1.0 -3.9 unbinned pixels; 13.0 9.5 nm along two shift axes; 15.4 -3.1 nm on specimen axes Total_Shift = 8.062239 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.31 microns changed by -2.29 to target drift = 0.11 nm/sec Measured defocus = -2.61 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4508.tif 27 frames are being saved to U:\krios\20190717\PilA_4509.tif 27 frames are being saved to U:\krios\20190717\PilA_4510.tif 27 frames are being saved to U:\krios\20190717\PilA_4511.tif 27 frames are being saved to U:\krios\20190717\PilA_4512.tif 27 frames are being saved to U:\krios\20190717\PilA_4513.tif Running script 17 at item 847 (-538.27, -81.38) Remaining time to fill dewars is 12060 Dewars are NOT busy filling Trimming A by 21 141 20 190, reference by 207 22 20 230 Trimming A by 21 141 20 190, reference by 229 24 160 20 Trimming A by 21 141 20 190, reference by 22 145 20 185 Trimming A by 21 141 20 190, reference by 21 140 135 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.08 Trimming A by 21 20 20 55, reference by 22 145 20 185 Disparity in stage position after aligning to target position: 0.02 0.09 (2nd move error 0.09 0.15) Finished aligning to item in 2 rounds Trimming A by 20 25 20 50, reference by 21 20 20 55 3.8 1.3 unbinned pixels; 0.3 15.8 nm along two shift axes; -5.8 14.7 nm on specimen axes Total_Shift = 8.104428 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.72 microns changed by -3.62 to target drift = 0.17 nm/sec Measured defocus = -0.96 microns changed by 0.06 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4514.tif 27 frames are being saved to U:\krios\20190717\PilA_4515.tif 27 frames are being saved to U:\krios\20190717\PilA_4516.tif 27 frames are being saved to U:\krios\20190717\PilA_4517.tif 27 frames are being saved to U:\krios\20190717\PilA_4518.tif 27 frames are being saved to U:\krios\20190717\PilA_4519.tif Running script 17 at item 848 (-535.66, -82.56) Remaining time to fill dewars is 11940 Dewars are NOT busy filling Trimming A by 20 145 140 20, reference by 207 22 20 230 Trimming A by 20 145 140 20, reference by 229 24 160 20 Trimming A by 20 145 140 20, reference by 22 145 20 185 Trimming A by 20 145 140 20, reference by 21 140 135 20 Disparity in stage position after aligning to center of map frame: -0.16 -0.04 Trimming A by 20 20 20 20, reference by 21 140 135 20 Disparity in stage position after aligning to target position: -0.26 -0.28 (2nd move error -0.10 -0.26) Finished aligning to item in 2 rounds -28.3 20.8 unbinned pixels; 113.5 70.4 nm along two shift axes; -75.0 -114.4 nm on specimen axes Total_Shift = 70.217452 -1.1 13.9 unbinned pixels; 52.5 16.8 nm along two shift axes; -53.7 -7.4 nm on specimen axes Total_Shift = 27.849593 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.53 microns changed by -3.53 to target drift = 0.04 nm/sec Measured defocus = -1.05 microns changed by 0.05 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4520.tif 27 frames are being saved to U:\krios\20190717\PilA_4521.tif 27 frames are being saved to U:\krios\20190717\PilA_4522.tif 27 frames are being saved to U:\krios\20190717\PilA_4523.tif 27 frames are being saved to U:\krios\20190717\PilA_4524.tif 27 frames are being saved to U:\krios\20190717\PilA_4525.tif Running script 17 at item 849 (-533.19, -84.31) Remaining time to fill dewars is 11700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 50 40 20, reference by 21 140 135 20 Disparity in stage position after aligning to target position: -0.18 -0.23 (2nd move error -0.02 -0.21) Finished aligning to item in 1 rounds Trimming A by 20 50 45 20, reference by 20 50 40 20 -26.1 5.9 unbinned pixels; 55.7 85.1 nm along two shift axes; -17.4 -102.8 nm on specimen axes Total_Shift = 53.508509 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.36 microns changed by -3.46 to target drift = 0.04 nm/sec Measured defocus = -1.15 microns changed by 0.05 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4526.tif 27 frames are being saved to U:\krios\20190717\PilA_4527.tif 27 frames are being saved to U:\krios\20190717\PilA_4528.tif 27 frames are being saved to U:\krios\20190717\PilA_4529.tif 27 frames are being saved to U:\krios\20190717\PilA_4530.tif 27 frames are being saved to U:\krios\20190717\PilA_4531.tif Running script 17 at item 850 (-530.58, -86.09) Remaining time to fill dewars is 11580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 106 120 20, reference by 21 140 135 20 Disparity in stage position after aligning to target position: -0.05 -0.13 (2nd move error 0.11 -0.11) Finished aligning to item in 1 rounds Trimming A by 21 120 115 20, reference by 22 106 120 20 -9.8 14.6 unbinned pixels; 66.4 13.5 nm along two shift axes; -54.6 -41.3 nm on specimen axes Total_Shift = 35.156648 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.15 microns changed by -3.35 to target drift = 0.09 nm/sec Measured defocus = -1.19 microns changed by -0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4532.tif 27 frames are being saved to U:\krios\20190717\PilA_4533.tif 27 frames are being saved to U:\krios\20190717\PilA_4534.tif 27 frames are being saved to U:\krios\20190717\PilA_4535.tif 27 frames are being saved to U:\krios\20190717\PilA_4536.tif 27 frames are being saved to U:\krios\20190717\PilA_4537.tif Running script 17 at item 851 (-532.23, -88.62) Remaining time to fill dewars is 11340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 135 100 20, reference by 21 140 135 20 Disparity in stage position after aligning to target position: -0.12 -0.20 (2nd move error 0.04 -0.18) Finished aligning to item in 1 rounds Trimming A by 24 140 95 20, reference by 24 135 100 20 -23.6 -1.3 unbinned pixels; 25.8 86.8 nm along two shift axes; 10.2 -91.4 nm on specimen axes Total_Shift = 47.193571 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.15 microns changed by -3.45 to target drift = 0.09 nm/sec Measured defocus = -1.38 microns changed by 0.08 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4538.tif 27 frames are being saved to U:\krios\20190717\PilA_4539.tif 27 frames are being saved to U:\krios\20190717\PilA_4540.tif 27 frames are being saved to U:\krios\20190717\PilA_4541.tif 27 frames are being saved to U:\krios\20190717\PilA_4542.tif 27 frames are being saved to U:\krios\20190717\PilA_4543.tif Running script 17 at item 852 (-534.79, -86.94) Remaining time to fill dewars is 11100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 80 20 60, reference by 21 140 135 20 Disparity in stage position after aligning to target position: -0.09 -0.31 (2nd move error 0.06 -0.29) Finished aligning to item in 1 rounds Trimming A by 20 80 20 65, reference by 24 80 20 60 -28.0 -3.2 unbinned pixels; 25.0 105.6 nm along two shift axes; 18.2 -108.4 nm on specimen axes Total_Shift = 56.399336 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.05 microns changed by -3.45 to target drift = 0.13 nm/sec Measured defocus = -1.48 microns changed by 0.08 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4544.tif 27 frames are being saved to U:\krios\20190717\PilA_4545.tif 27 frames are being saved to U:\krios\20190717\PilA_4546.tif 27 frames are being saved to U:\krios\20190717\PilA_4547.tif 27 frames are being saved to U:\krios\20190717\PilA_4548.tif 27 frames are being saved to U:\krios\20190717\PilA_4549.tif Running script 17 at item 853 (-537.41, -85.69) Remaining time to fill dewars is 10920 Dewars are NOT busy filling Trimming A by 21 141 20 190, reference by 207 22 20 230 Trimming A by 21 141 20 190, reference by 229 24 160 20 Trimming A by 21 141 20 190, reference by 22 145 20 185 Trimming A by 21 141 20 190, reference by 21 140 135 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.07 Trimming A by 20 80 20 85, reference by 22 145 20 185 Disparity in stage position after aligning to target position: 0.03 0.11 (2nd move error 0.11 0.18) Finished aligning to item in 2 rounds Trimming A by 20 75 20 80, reference by 20 80 20 85 2.4 2.3 unbinned pixels; 5.3 12.3 nm along two shift axes; -9.5 9.0 nm on specimen axes Total_Shift = 6.742255 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.08 microns changed by -3.58 to target drift = 0.15 nm/sec Measured defocus = -1.51 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4550.tif 27 frames are being saved to U:\krios\20190717\PilA_4551.tif 27 frames are being saved to U:\krios\20190717\PilA_4552.tif 27 frames are being saved to U:\krios\20190717\PilA_4553.tif 27 frames are being saved to U:\krios\20190717\PilA_4554.tif 27 frames are being saved to U:\krios\20190717\PilA_4555.tif Running script 17 at item 854 (-542.41, -82.19) Remaining time to fill dewars is 10740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 22 20 155, reference by 22 145 20 185 Disparity in stage position after aligning to target position: -0.11 -0.06 (2nd move error -0.04 0.01) Finished aligning to item in 1 rounds Trimming A by 43 21 20 150, reference by 35 22 20 155 1.7 8.3 unbinned pixels; 28.2 18.5 nm along two shift axes; -32.5 4.8 nm on specimen axes Total_Shift = 16.872314 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.84 microns changed by -2.44 to target drift = 0.13 nm/sec Measured defocus = -1.63 microns changed by 0.03 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4556.tif 27 frames are being saved to U:\krios\20190717\PilA_4557.tif 27 frames are being saved to U:\krios\20190717\PilA_4558.tif 27 frames are being saved to U:\krios\20190717\PilA_4559.tif 27 frames are being saved to U:\krios\20190717\PilA_4560.tif 27 frames are being saved to U:\krios\20190717\PilA_4561.tif Running script 17 at item 855 (-541.51, -86.54) Remaining time to fill dewars is 10500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 130 20 170, reference by 22 145 20 185 Disparity in stage position after aligning to target position: -0.01 -0.04 (2nd move error 0.06 0.01) Finished aligning to item in 1 rounds Trimming A by 21 121 20 175, reference by 21 130 20 170 1.8 -1.3 unbinned pixels; 7.2 4.5 nm along two shift axes; 4.8 7.3 nm on specimen axes Total_Shift = 4.476046 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.91 microns changed by -3.61 to target drift = 0.11 nm/sec Measured defocus = -1.78 microns changed by 0.08 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4562.tif 27 frames are being saved to U:\krios\20190717\PilA_4563.tif 27 frames are being saved to U:\krios\20190717\PilA_4564.tif 27 frames are being saved to U:\krios\20190717\PilA_4565.tif 27 frames are being saved to U:\krios\20190717\PilA_4566.tif 27 frames are being saved to U:\krios\20190717\PilA_4567.tif Running script 17 at item 856 (-539.07, -88.24) Remaining time to fill dewars is 10380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 150 20 145, reference by 22 145 20 185 Disparity in stage position after aligning to target position: 0.08 0.05 (2nd move error 0.15 0.11) Finished aligning to item in 1 rounds Trimming A by 20 150 20 140, reference by 21 150 20 145 -2.1 -4.9 unbinned pixels; 15.3 15.0 nm along two shift axes; 19.5 -7.1 nm on specimen axes Total_Shift = 10.677551 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.38 microns changed by -2.18 to target drift = 0.12 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4568.tif 27 frames are being saved to U:\krios\20190717\PilA_4569.tif 27 frames are being saved to U:\krios\20190717\PilA_4570.tif 27 frames are being saved to U:\krios\20190717\PilA_4571.tif 27 frames are being saved to U:\krios\20190717\PilA_4572.tif 27 frames are being saved to U:\krios\20190717\PilA_4573.tif Running script 17 at item 857 (-536.51, -89.97) Remaining time to fill dewars is 10140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 160 20 135, reference by 22 145 20 185 Disparity in stage position after aligning to target position: 0.11 0.06 (2nd move error 0.19 0.13) Finished aligning to item in 1 rounds Trimming A by 23 155 20 130, reference by 21 160 20 135 5.0 -13.4 unbinned pixels; 55.6 2.3 nm along two shift axes; 51.0 22.1 nm on specimen axes Total_Shift = 28.514197 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.50 microns changed by -3.40 to target drift = 0.16 nm/sec Measured defocus = -1.93 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4574.tif 27 frames are being saved to U:\krios\20190717\PilA_4575.tif 27 frames are being saved to U:\krios\20190717\PilA_4576.tif 27 frames are being saved to U:\krios\20190717\PilA_4577.tif 27 frames are being saved to U:\krios\20190717\PilA_4578.tif 27 frames are being saved to U:\krios\20190717\PilA_4579.tif Running script 17 at item 867-1 (-890.57, -95.93) Remaining time to fill dewars is 9960 Dewars are NOT busy filling Trimming A by 250 23 210 20, reference by 228 21 20 260 Trimming A by 250 23 210 20, reference by 239 22 205 20 Trimming A by 250 23 210 20, reference by 20 240 20 250 Trimming A by 250 23 210 20, reference by 22 239 205 20 Disparity in stage position after aligning to center of map frame: -0.18 -0.17 Trimming A by 90 65 140 20, reference by 239 22 205 20 Disparity in stage position after aligning to target position: -0.10 -0.21 (2nd move error 0.07 -0.07) Finished aligning to item in 2 rounds Trimming A by 90 61 145 20, reference by 90 65 140 20 -8.4 -0.3 unbinned pixels; 9.8 30.5 nm along two shift axes; 2.9 -32.5 nm on specimen axes Total_Shift = 16.726417 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.34 microns changed by -2.34 to target drift = 0.10 nm/sec Measured defocus = -2.03 microns changed by 0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4580.tif 27 frames are being saved to U:\krios\20190717\PilA_4581.tif 27 frames are being saved to U:\krios\20190717\PilA_4582.tif 27 frames are being saved to U:\krios\20190717\PilA_4583.tif 27 frames are being saved to U:\krios\20190717\PilA_4584.tif 27 frames are being saved to U:\krios\20190717\PilA_4585.tif Running script 17 at item 868-1 (-893.11, -94.32) Remaining time to fill dewars is 9780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 110 24 100 20, reference by 239 22 205 20 Disparity in stage position after aligning to target position: -0.16 -0.28 (2nd move error 0.01 -0.14) Finished aligning to item in 1 rounds Trimming A by 105 24 100 20, reference by 110 24 100 20 -17.2 -0.4 unbinned pixels; 20.8 62.6 nm along two shift axes; 5.4 -66.9 nm on specimen axes Total_Shift = 34.437053 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.70 microns changed by -3.80 to target drift = 0.08 nm/sec Measured defocus = -2.21 microns changed by 0.11 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4586.tif 27 frames are being saved to U:\krios\20190717\PilA_4587.tif 27 frames are being saved to U:\krios\20190717\PilA_4588.tif 27 frames are being saved to U:\krios\20190717\PilA_4589.tif 27 frames are being saved to U:\krios\20190717\PilA_4590.tif 27 frames are being saved to U:\krios\20190717\PilA_4591.tif Running script 17 at item 869-1 (-895.60, -92.58) Remaining time to fill dewars is 9600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 20 75 65, reference by 239 22 205 20 Disparity in stage position after aligning to target position: -0.14 -0.33 (2nd move error 0.03 -0.19) Finished aligning to item in 1 rounds Trimming A by 130 20 70 60, reference by 130 20 75 65 -22.6 -3.4 unbinned pixels; 17.1 86.5 nm along two shift axes; 17.9 -87.3 nm on specimen axes Total_Shift = 45.739702 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.30 microns changed by -2.50 to target drift = 0.10 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4592.tif 27 frames are being saved to U:\krios\20190717\PilA_4593.tif 27 frames are being saved to U:\krios\20190717\PilA_4594.tif 27 frames are being saved to U:\krios\20190717\PilA_4595.tif 27 frames are being saved to U:\krios\20190717\PilA_4596.tif 27 frames are being saved to U:\krios\20190717\PilA_4597.tif Running script 17 at item 870-1 (-899.91, -93.92) Remaining time to fill dewars is 9420 Dewars are NOT busy filling Trimming A by 245 24 20 250, reference by 228 21 20 260 Trimming A by 245 24 20 250, reference by 239 22 205 20 Trimming A by 245 24 20 250, reference by 20 240 20 250 Trimming A by 245 24 20 250, reference by 22 239 205 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.10 Trimming A by 140 21 20 160, reference by 228 21 20 260 Disparity in stage position after aligning to target position: -0.05 -0.01 (2nd move error 0.08 0.06) Finished aligning to item in 2 rounds Trimming A by 127 24 20 170, reference by 140 21 20 160 0.5 -0.9 unbinned pixels; 4.0 0.4 nm along two shift axes; 3.4 2.1 nm on specimen axes Total_Shift = 2.076488 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.58 microns changed by -3.88 to target drift = 0.18 nm/sec Measured defocus = -2.28 microns changed by -0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4598.tif 27 frames are being saved to U:\krios\20190717\PilA_4599.tif 27 frames are being saved to U:\krios\20190717\PilA_4600.tif 27 frames are being saved to U:\krios\20190717\PilA_4601.tif 27 frames are being saved to U:\krios\20190717\PilA_4602.tif 27 frames are being saved to U:\krios\20190717\PilA_4603.tif Running script 17 at item 871-1 (-897.28, -95.60) Remaining time to fill dewars is 9180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 62 22 20 100, reference by 228 21 20 260 Disparity in stage position after aligning to target position: 0.16 -0.01 (2nd move error 0.28 0.06) Finished aligning to item in 1 rounds Trimming A by 75 21 20 80, reference by 62 22 20 100 2.9 7.8 unbinned pixels; 24.8 22.2 nm along two shift axes; -30.9 9.7 nm on specimen axes Total_Shift = 16.632992 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.12 microns changed by -2.52 to target drift = 0.14 nm/sec Measured defocus = -2.40 microns changed by -0.00 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4604.tif 27 frames are being saved to U:\krios\20190717\PilA_4605.tif 27 frames are being saved to U:\krios\20190717\PilA_4606.tif 27 frames are being saved to U:\krios\20190717\PilA_4607.tif 27 frames are being saved to U:\krios\20190717\PilA_4608.tif 27 frames are being saved to U:\krios\20190717\PilA_4609.tif Running script 17 at item 872-1 (-894.67, -96.76) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 40 40 20, reference by 239 22 205 20 Disparity in stage position after aligning to target position: -0.10 -0.28 (2nd move error 0.07 -0.13) Finished aligning to item in 1 rounds Trimming A by 25 26 55 30, reference by 35 40 40 20 -15.5 0.3 unbinned pixels; 21.3 55.2 nm along two shift axes; 2.1 -60.3 nm on specimen axes Total_Shift = 30.953218 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.09 microns changed by -2.41 to target drift = 0.13 nm/sec Measured defocus = -2.51 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4610.tif 27 frames are being saved to U:\krios\20190717\PilA_4611.tif 27 frames are being saved to U:\krios\20190717\PilA_4612.tif 27 frames are being saved to U:\krios\20190717\PilA_4613.tif 27 frames are being saved to U:\krios\20190717\PilA_4614.tif 27 frames are being saved to U:\krios\20190717\PilA_4615.tif Running script 17 at item 873-1 (-892.04, -98.48) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Trimming A by 23 245 205 20, reference by 228 21 20 260 Trimming A by 23 245 205 20, reference by 239 22 205 20 Trimming A by 23 245 205 20, reference by 20 240 20 250 Trimming A by 23 245 205 20, reference by 22 239 205 20 Disparity in stage position after aligning to center of map frame: -0.10 -0.12 Trimming A by 20 100 120 20, reference by 22 239 205 20 Disparity in stage position after aligning to target position: -0.23 -0.30 (2nd move error -0.15 -0.21) Finished aligning to item in 2 rounds Trimming A by 21 100 120 20, reference by 20 100 120 20 -23.4 27.8 unbinned pixels; 132.8 42.2 nm along two shift axes; -103.3 -96.8 nm on specimen axes Total_Shift = 72.678886 Trimming A by 21 100 120 20, reference by 20 100 120 20 1.0 16.6 unbinned pixels; 59.6 28.6 nm along two shift axes; -64.6 0.5 nm on specimen axes Total_Shift = 33.187923 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.27 microns changed by -2.33 to target drift = 0.03 nm/sec Measured defocus = -2.57 microns changed by -0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4616.tif 27 frames are being saved to U:\krios\20190717\PilA_4617.tif 27 frames are being saved to U:\krios\20190717\PilA_4618.tif 27 frames are being saved to U:\krios\20190717\PilA_4619.tif 27 frames are being saved to U:\krios\20190717\PilA_4620.tif 27 frames are being saved to U:\krios\20190717\PilA_4621.tif Running script 17 at item 874-1 (-893.66, -101.17) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 140 90 35, reference by 22 239 205 20 Disparity in stage position after aligning to target position: -0.17 -0.36 (2nd move error -0.08 -0.27) Finished aligning to item in 1 rounds Trimming A by 22 145 90 35, reference by 20 140 90 35 -24.1 16.9 unbinned pixels; 93.6 61.4 nm along two shift axes; -60.6 -97.4 nm on specimen axes Total_Shift = 58.887479 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.69 microns changed by -2.59 to target drift = 0.03 nm/sec Measured defocus = -0.94 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4622.tif 27 frames are being saved to U:\krios\20190717\PilA_4623.tif 27 frames are being saved to U:\krios\20190717\PilA_4624.tif 27 frames are being saved to U:\krios\20190717\PilA_4625.tif 27 frames are being saved to U:\krios\20190717\PilA_4626.tif 27 frames are being saved to U:\krios\20190717\PilA_4627.tif Running script 17 at item 875 (-896.27, -99.84) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 21 240 50 245, reference by 228 21 20 260 Trimming A by 21 240 50 245, reference by 239 22 205 20 Trimming A by 21 240 50 245, reference by 20 240 20 250 Trimming A by 21 240 50 245, reference by 22 239 205 20 Disparity in stage position after aligning to center of map frame: -0.15 -0.13 Trimming A by 23 110 20 90, reference by 20 240 20 250 Disparity in stage position after aligning to target position: -0.14 0.10 (2nd move error -0.01 0.20) Finished aligning to item in 2 rounds Trimming A by 24 105 20 90, reference by 23 110 20 90 -2.0 13.3 unbinned pixels; 51.6 12.8 nm along two shift axes; -51.4 -10.7 nm on specimen axes Total_Shift = 26.956588 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.95 microns changed by -3.95 to target drift = 0.18 nm/sec Measured defocus = -1.05 microns changed by 0.05 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4628.tif 27 frames are being saved to U:\krios\20190717\PilA_4629.tif 27 frames are being saved to U:\krios\20190717\PilA_4630.tif 27 frames are being saved to U:\krios\20190717\PilA_4631.tif 27 frames are being saved to U:\krios\20190717\PilA_4632.tif 27 frames are being saved to U:\krios\20190717\PilA_4633.tif Running script 17 at item 876 (-898.73, -98.24) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 80 20 125, reference by 20 240 20 250 Disparity in stage position after aligning to target position: -0.09 -0.02 (2nd move error 0.05 0.08) Finished aligning to item in 1 rounds Trimming A by 22 67 20 135, reference by 21 80 20 125 -0.5 -2.2 unbinned pixels; 7.4 5.2 nm along two shift axes; 8.6 -1.6 nm on specimen axes Total_Shift = 4.507732 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.47 microns changed by -2.57 to target drift = 0.14 nm/sec Measured defocus = -1.07 microns changed by -0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4634.tif 27 frames are being saved to U:\krios\20190717\PilA_4635.tif 27 frames are being saved to U:\krios\20190717\PilA_4636.tif 27 frames are being saved to U:\krios\20190717\PilA_4637.tif 27 frames are being saved to U:\krios\20190717\PilA_4638.tif 27 frames are being saved to U:\krios\20190717\PilA_4639.tif Running script 17 at item 877 (-502.01, -21.10) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 270 23 200 20, reference by 270 20 20 260 Trimming A by 270 23 200 20, reference by 258 23 200 20 Trimming A by 270 23 200 20, reference by 23 155 20 215 Trimming A by 270 23 200 20, reference by 20 114 185 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.08 Trimming A by 140 24 140 20, reference by 258 23 200 20 Disparity in stage position after aligning to target position: -0.18 -0.19 (2nd move error 0.04 -0.13) Finished aligning to item in 2 rounds Trimming A by 140 23 135 20, reference by 140 24 140 20 -18.1 -0.4 unbinned pixels; 22.0 65.8 nm along two shift axes; 5.5 -70.3 nm on specimen axes Total_Shift = 36.202943 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.35 microns changed by -3.55 to target drift = 0.15 nm/sec Measured defocus = -1.18 microns changed by -0.02 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4640.tif 27 frames are being saved to U:\krios\20190717\PilA_4641.tif 27 frames are being saved to U:\krios\20190717\PilA_4642.tif 27 frames are being saved to U:\krios\20190717\PilA_4643.tif 27 frames are being saved to U:\krios\20190717\PilA_4644.tif 27 frames are being saved to U:\krios\20190717\PilA_4645.tif Running script 17 at item 878 (-501.08, -25.33) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 22 118 190 20, reference by 270 20 20 260 Trimming A by 22 118 190 20, reference by 258 23 200 20 Trimming A by 22 118 190 20, reference by 23 155 20 215 Trimming A by 22 118 190 20, reference by 20 114 185 20 Disparity in stage position after aligning to center of map frame: -0.21 -0.13 Trimming A by 36 40 125 20, reference by 20 114 185 20 Disparity in stage position after aligning to target position: -0.21 -0.19 (2nd move error -0.01 -0.09) Finished aligning to item in 2 rounds Trimming A by 27 37 130 20, reference by 36 40 125 20 -12.9 5.5 unbinned pixels; 37.0 38.3 nm along two shift axes; -18.6 -51.4 nm on specimen axes Total_Shift = 28.074207 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.15 microns changed by -3.45 to target drift = 0.06 nm/sec Measured defocus = -1.30 microns changed by 0.00 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4646.tif 27 frames are being saved to U:\krios\20190717\PilA_4647.tif 27 frames are being saved to U:\krios\20190717\PilA_4648.tif 27 frames are being saved to U:\krios\20190717\PilA_4649.tif 27 frames are being saved to U:\krios\20190717\PilA_4650.tif 27 frames are being saved to U:\krios\20190717\PilA_4651.tif Running script 17 at item 879 (-506.17, -21.94) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 80 20 50 60, reference by 258 23 200 20 Disparity in stage position after aligning to target position: -0.22 -0.22 (2nd move error -0.00 -0.17) Finished aligning to item in 1 rounds Trimming A by 80 20 50 60, reference by 80 20 50 60 -22.6 3.2 unbinned pixels; 41.2 76.7 nm along two shift axes; -7.5 -88.7 nm on specimen axes Total_Shift = 45.704137 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.42 microns changed by -3.82 to target drift = 0.03 nm/sec Measured defocus = -1.50 microns changed by 0.10 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4652.tif 27 frames are being saved to U:\krios\20190717\PilA_4653.tif 27 frames are being saved to U:\krios\20190717\PilA_4654.tif 27 frames are being saved to U:\krios\20190717\PilA_4655.tif 27 frames are being saved to U:\krios\20190717\PilA_4656.tif 27 frames are being saved to U:\krios\20190717\PilA_4657.tif Running script 17 at item 880 (-508.85, -20.66) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Trimming A by 270 20 20 260, reference by 270 20 20 260 Trimming A by 270 20 20 260, reference by 258 23 200 20 Trimming A by 270 20 20 260, reference by 23 155 20 215 Trimming A by 270 20 20 260, reference by 20 114 185 20 Disparity in stage position after aligning to center of map frame: -0.12 -0.04 Trimming A by 145 22 20 120, reference by 270 20 20 260 Disparity in stage position after aligning to target position: 0.08 0.12 (2nd move error 0.19 0.14) Finished aligning to item in 2 rounds Trimming A by 145 22 20 120, reference by 145 22 20 120 -1.1 0.3 unbinned pixels; 2.7 3.6 nm along two shift axes; -1.0 -4.5 nm on specimen axes Total_Shift = 2.358 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.98 microns changed by -2.48 to target drift = 0.14 nm/sec Measured defocus = -1.45 microns changed by -0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4658.tif 27 frames are being saved to U:\krios\20190717\PilA_4659.tif 27 frames are being saved to U:\krios\20190717\PilA_4660.tif 27 frames are being saved to U:\krios\20190717\PilA_4661.tif 27 frames are being saved to U:\krios\20190717\PilA_4662.tif 27 frames are being saved to U:\krios\20190717\PilA_4663.tif Running script 17 at item 881 (-510.49, -23.18) Remaining time to fill dewars is 7740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 97 21 20 155, reference by 270 20 20 260 Disparity in stage position after aligning to target position: 0.03 -0.01 (2nd move error 0.15 0.00) Finished aligning to item in 1 rounds Trimming A by 106 20 20 145, reference by 97 21 20 155 0.7 0.2 unbinned pixels; 0.3 2.6 nm along two shift axes; -0.7 2.6 nm on specimen axes Total_Shift = 1.370431 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.15 microns changed by -3.75 to target drift = 0.11 nm/sec Measured defocus = -1.60 microns changed by -0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4664.tif 27 frames are being saved to U:\krios\20190717\PilA_4665.tif 27 frames are being saved to U:\krios\20190717\PilA_4666.tif 27 frames are being saved to U:\krios\20190717\PilA_4667.tif 27 frames are being saved to U:\krios\20190717\PilA_4668.tif 27 frames are being saved to U:\krios\20190717\PilA_4669.tif Running script 17 at item 882 (-507.73, -24.68) Remaining time to fill dewars is 7560 Dewars are NOT busy filling Trimming A by 23 155 20 215, reference by 270 20 20 260 Trimming A by 23 155 20 215, reference by 258 23 200 20 Trimming A by 23 155 20 215, reference by 23 155 20 215 Trimming A by 23 155 20 215, reference by 20 114 185 20 Disparity in stage position after aligning to center of map frame: -0.10 -0.09 Trimming A by 40 20 20 70, reference by 23 155 20 215 Disparity in stage position after aligning to target position: -0.15 0.14 (2nd move error -0.07 0.21) Finished aligning to item in 2 rounds Trimming A by 41 21 20 75, reference by 40 20 20 70 1.4 13.5 unbinned pixels; 48.0 25.3 nm along two shift axes; -53.0 2.5 nm on specimen axes Total_Shift = 27.226302 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.68 microns changed by -2.38 to target drift = 0.17 nm/sec Measured defocus = -1.74 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4670.tif 27 frames are being saved to U:\krios\20190717\PilA_4671.tif 27 frames are being saved to U:\krios\20190717\PilA_4672.tif 27 frames are being saved to U:\krios\20190717\PilA_4673.tif 27 frames are being saved to U:\krios\20190717\PilA_4674.tif 27 frames are being saved to U:\krios\20190717\PilA_4675.tif Running script 17 at item 883 (-505.15, -26.21) Remaining time to fill dewars is 7320 Dewars are NOT busy filling Trimming A by 20 114 190 20, reference by 270 20 20 260 Trimming A by 20 114 190 20, reference by 258 23 200 20 Trimming A by 20 114 190 20, reference by 23 155 20 215 Trimming A by 20 114 190 20, reference by 20 114 185 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.07 Trimming A by 20 30 20 20, reference by 20 114 185 20 Disparity in stage position after aligning to target position: -0.20 -0.25 (2nd move error -0.06 -0.20) Finished aligning to item in 2 rounds Trimming A by 20 30 25 20, reference by 20 30 20 20 -24.2 12.4 unbinned pixels; 77.3 68.4 nm along two shift axes; -43.3 -96.7 nm on specimen axes Total_Shift = 54.407498 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.87 microns changed by -3.67 to target drift = 0.04 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4676.tif 27 frames are being saved to U:\krios\20190717\PilA_4677.tif 27 frames are being saved to U:\krios\20190717\PilA_4678.tif 27 frames are being saved to U:\krios\20190717\PilA_4679.tif 27 frames are being saved to U:\krios\20190717\PilA_4680.tif 27 frames are being saved to U:\krios\20190717\PilA_4681.tif Running script 17 at item 884 (-502.64, -27.92) Remaining time to fill dewars is 7080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 57 110 20, reference by 20 114 185 20 Disparity in stage position after aligning to target position: -0.09 -0.20 (2nd move error 0.04 -0.14) Finished aligning to item in 1 rounds Trimming A by 22 52 115 20, reference by 20 57 110 20 -15.5 -0.1 unbinned pixels; 19.8 56.0 nm along two shift axes; 3.8 -60.3 nm on specimen axes Total_Shift = 31.004019 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.30 microns changed by -2.20 to target drift = 0.12 nm/sec Measured defocus = -1.93 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4682.tif 27 frames are being saved to U:\krios\20190717\PilA_4683.tif 27 frames are being saved to U:\krios\20190717\PilA_4684.tif 27 frames are being saved to U:\krios\20190717\PilA_4685.tif 27 frames are being saved to U:\krios\20190717\PilA_4686.tif 27 frames are being saved to U:\krios\20190717\PilA_4687.tif Running script 17 at item 885 (-501.71, -32.26) Remaining time to fill dewars is 6900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 156 155 20, reference by 20 114 185 20 Disparity in stage position after aligning to target position: -0.03 -0.15 (2nd move error 0.10 -0.10) Finished aligning to item in 1 rounds Trimming A by 20 157 160 20, reference by 23 156 155 20 -9.1 1.3 unbinned pixels; 16.9 30.9 nm along two shift axes; -3.3 -35.9 nm on specimen axes Total_Shift = 18.484533 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.45 microns changed by -3.45 to target drift = 0.07 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4688.tif 27 frames are being saved to U:\krios\20190717\PilA_4689.tif 27 frames are being saved to U:\krios\20190717\PilA_4690.tif 27 frames are being saved to U:\krios\20190717\PilA_4691.tif 27 frames are being saved to U:\krios\20190717\PilA_4692.tif 27 frames are being saved to U:\krios\20190717\PilA_4693.tif Running script 17 at item 886 (-504.27, -30.57) Remaining time to fill dewars is 6660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 105 70 30, reference by 20 114 185 20 Disparity in stage position after aligning to target position: -0.09 -0.25 (2nd move error 0.05 -0.20) Finished aligning to item in 1 rounds Trimming A by 20 105 75 35, reference by 24 105 70 30 -24.4 1.5 unbinned pixels; 37.4 85.6 nm along two shift axes; -0.7 -95.2 nm on specimen axes Total_Shift = 48.876479 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.16 microns changed by -2.26 to target drift = 0.10 nm/sec Measured defocus = -2.14 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4694.tif 27 frames are being saved to U:\krios\20190717\PilA_4695.tif 27 frames are being saved to U:\krios\20190717\PilA_4696.tif 27 frames are being saved to U:\krios\20190717\PilA_4697.tif 27 frames are being saved to U:\krios\20190717\PilA_4698.tif 27 frames are being saved to U:\krios\20190717\PilA_4699.tif Running script 17 at item 887 (-506.80, -28.83) Remaining time to fill dewars is 6480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 70 20 70, reference by 20 114 185 20 Disparity in stage position after aligning to target position: -0.15 -0.33 (2nd move error -0.02 -0.28) Finished aligning to item in 1 rounds Trimming A by 24 75 20 70, reference by 20 70 20 70 -36.5 14.2 unbinned pixels; 99.9 110.1 nm along two shift axes; -47.6 -145.1 nm on specimen axes Total_Shift = 78.398509 Trimming A by 24 80 20 70, reference by 20 70 20 70 0.8 8.4 unbinned pixels; 29.8 15.5 nm along two shift axes; -32.8 1.3 nm on specimen axes Total_Shift = 16.871546 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.44 microns changed by -3.64 to target drift = 0.03 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4700.tif 27 frames are being saved to U:\krios\20190717\PilA_4701.tif 27 frames are being saved to U:\krios\20190717\PilA_4702.tif 27 frames are being saved to U:\krios\20190717\PilA_4703.tif 27 frames are being saved to U:\krios\20190717\PilA_4704.tif 27 frames are being saved to U:\krios\20190717\PilA_4705.tif Running script 17 at item 888 (-509.28, -27.51) Remaining time to fill dewars is 6300 Dewars are NOT busy filling Trimming A by 23 155 20 215, reference by 270 20 20 260 Trimming A by 23 155 20 215, reference by 258 23 200 20 Trimming A by 23 155 20 215, reference by 23 155 20 215 Trimming A by 23 155 20 215, reference by 20 114 185 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.09 Trimming A by 21 60 20 100, reference by 23 155 20 215 Disparity in stage position after aligning to target position: -0.07 0.05 (2nd move error 0.01 0.12) Finished aligning to item in 2 rounds Trimming A by 20 51 20 105, reference by 21 60 20 100 -1.2 1.6 unbinned pixels; 7.5 2.0 nm along two shift axes; -6.0 -5.1 nm on specimen axes Total_Shift = 4.020016 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.07 microns changed by -2.37 to target drift = 0.15 nm/sec Measured defocus = -2.24 microns changed by -0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4706.tif 27 frames are being saved to U:\krios\20190717\PilA_4707.tif 27 frames are being saved to U:\krios\20190717\PilA_4708.tif 27 frames are being saved to U:\krios\20190717\PilA_4709.tif 27 frames are being saved to U:\krios\20190717\PilA_4710.tif 27 frames are being saved to U:\krios\20190717\PilA_4711.tif Running script 17 at item 889 (-511.78, -25.81) Remaining time to fill dewars is 6120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 51 23 20 175, reference by 23 155 20 215 Disparity in stage position after aligning to target position: -0.18 -0.06 (2nd move error -0.09 0.01) Finished aligning to item in 1 rounds Trimming A by 51 23 20 175, reference by 51 23 20 175 3.9 17.5 unbinned pixels; 59.2 40.4 nm along two shift axes; -68.8 11.5 nm on specimen axes Total_Shift = 35.832725 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.37 microns changed by -3.77 to target drift = 0.15 nm/sec Measured defocus = -2.44 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4712.tif 27 frames are being saved to U:\krios\20190717\PilA_4713.tif 27 frames are being saved to U:\krios\20190717\PilA_4714.tif 27 frames are being saved to U:\krios\20190717\PilA_4715.tif 27 frames are being saved to U:\krios\20190717\PilA_4716.tif 27 frames are being saved to U:\krios\20190717\PilA_4717.tif Running script 17 at item 890 (-510.89, -30.15) Remaining time to fill dewars is 5940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 135 20 165, reference by 23 155 20 215 Disparity in stage position after aligning to target position: -0.01 -0.03 (2nd move error 0.07 0.04) Finished aligning to item in 1 rounds Trimming A by 20 130 20 165, reference by 20 135 20 165 -2.3 -0.7 unbinned pixels; 0.3 9.2 nm along two shift axes; 3.3 -8.6 nm on specimen axes Total_Shift = 4.747544 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.15 microns changed by -2.35 to target drift = 0.13 nm/sec Measured defocus = -2.53 microns changed by 0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4718.tif 27 frames are being saved to U:\krios\20190717\PilA_4719.tif 27 frames are being saved to U:\krios\20190717\PilA_4720.tif 27 frames are being saved to U:\krios\20190717\PilA_4721.tif 27 frames are being saved to U:\krios\20190717\PilA_4722.tif 27 frames are being saved to U:\krios\20190717\PilA_4723.tif Running script 17 at item 891 (-508.41, -31.80) Remaining time to fill dewars is 5760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 155 70 135, reference by 23 155 20 215 Disparity in stage position after aligning to target position: 0.01 0.07 (2nd move error 0.09 0.13) Finished aligning to item in 1 rounds Trimming A by 20 150 20 135, reference by 21 155 70 135 0.8 1.5 unbinned pixels; 4.5 5.2 nm along two shift axes; -6.1 2.9 nm on specimen axes Total_Shift = 3.443582 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 1.01 microns changed by -3.61 to target drift = 0.13 nm/sec Measured defocus = -2.64 microns changed by 0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4724.tif 27 frames are being saved to U:\krios\20190717\PilA_4725.tif 27 frames are being saved to U:\krios\20190717\PilA_4726.tif 27 frames are being saved to U:\krios\20190717\PilA_4727.tif 27 frames are being saved to U:\krios\20190717\PilA_4728.tif 27 frames are being saved to U:\krios\20190717\PilA_4729.tif Running script 17 at item 892 (-505.85, -33.13) Remaining time to fill dewars is 5520 Dewars are NOT busy filling Trimming A by 20 118 185 20, reference by 270 20 20 260 Trimming A by 20 118 185 20, reference by 258 23 200 20 Trimming A by 20 118 185 20, reference by 23 155 20 215 Trimming A by 20 118 185 20, reference by 20 114 185 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.05 Trimming A by 23 165 25 110, reference by 20 114 185 20 Disparity in stage position after aligning to target position: -0.05 -0.29 (2nd move error 0.04 -0.26) Finished aligning to item in 2 rounds Trimming A by 22 170 30 110, reference by 23 165 25 110 -24.7 -1.9 unbinned pixels; 25.2 91.7 nm along two shift axes; 12.6 -95.6 nm on specimen axes Total_Shift = 49.486867 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.42 microns changed by -2.32 to target drift = 0.15 nm/sec Measured defocus = -0.91 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4730.tif 27 frames are being saved to U:\krios\20190717\PilA_4731.tif 27 frames are being saved to U:\krios\20190717\PilA_4732.tif 27 frames are being saved to U:\krios\20190717\PilA_4733.tif 27 frames are being saved to U:\krios\20190717\PilA_4734.tif 27 frames are being saved to U:\krios\20190717\PilA_4735.tif Running script 17 at item 893 (-224.31, -35.46) Remaining time to fill dewars is 5400 Dewars are NOT busy filling Trimming A by 22 160 165 20, reference by 231 22 20 240 Trimming A by 22 160 165 20, reference by 239 20 185 20 Trimming A by 22 160 165 20, reference by 22 170 20 205 Trimming A by 22 160 165 20, reference by 21 150 165 25 Disparity in stage position after aligning to center of map frame: -0.27 -0.12 Trimming A by 41 39 150 20, reference by 21 150 165 25 Disparity in stage position after aligning to target position: -0.39 -0.08 (2nd move error -0.13 0.02) Finished aligning to item in 2 rounds Trimming A by 41 41 150 20, reference by 41 39 150 20 2.7 22.4 unbinned pixels; 78.7 43.4 nm along two shift axes; -87.5 5.8 nm on specimen axes Total_Shift = 45.028014 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.67 microns changed by -2.67 to target drift = 0.22 nm/sec Measured defocus = -1.03 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4736.tif 27 frames are being saved to U:\krios\20190717\PilA_4737.tif 27 frames are being saved to U:\krios\20190717\PilA_4738.tif 27 frames are being saved to U:\krios\20190717\PilA_4739.tif 27 frames are being saved to U:\krios\20190717\PilA_4740.tif 27 frames are being saved to U:\krios\20190717\PilA_4741.tif Running script 17 at item 894 (-225.91, -38.09) Remaining time to fill dewars is 5220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 86 120 20, reference by 21 150 165 25 Disparity in stage position after aligning to target position: -0.19 -0.19 (2nd move error 0.08 -0.08) Finished aligning to item in 1 rounds Trimming A by 24 77 130 20, reference by 24 86 120 20 -9.6 -2.4 unbinned pixels; 3.8 38.0 nm along two shift axes; 11.2 -36.7 nm on specimen axes Total_Shift = 19.713376 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.55 microns changed by -2.65 to target drift = 0.09 nm/sec Measured defocus = -1.11 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4742.tif 27 frames are being saved to U:\krios\20190717\PilA_4743.tif 27 frames are being saved to U:\krios\20190717\PilA_4744.tif 27 frames are being saved to U:\krios\20190717\PilA_4745.tif 27 frames are being saved to U:\krios\20190717\PilA_4746.tif 27 frames are being saved to U:\krios\20190717\PilA_4747.tif Running script 17 at item 895 (-227.53, -40.68) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 115 95 20, reference by 21 150 165 25 Disparity in stage position after aligning to target position: -0.22 -0.25 (2nd move error 0.04 -0.15) Finished aligning to item in 1 rounds Trimming A by 22 120 95 20, reference by 22 115 95 20 -15.9 -0.8 unbinned pixels; 17.9 58.5 nm along two shift axes; 6.4 -61.8 nm on specimen axes Total_Shift = 31.897721 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.46 microns changed by -2.66 to target drift = 0.10 nm/sec Measured defocus = -1.28 microns changed by 0.08 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4748.tif 27 frames are being saved to U:\krios\20190717\PilA_4749.tif 27 frames are being saved to U:\krios\20190717\PilA_4750.tif 27 frames are being saved to U:\krios\20190717\PilA_4751.tif 27 frames are being saved to U:\krios\20190717\PilA_4752.tif 27 frames are being saved to U:\krios\20190717\PilA_4753.tif Running script 17 at item 896 (-231.56, -41.94) Remaining time to fill dewars is 4860 Dewars are NOT busy filling Trimming A by 20 170 20 205, reference by 231 22 20 240 Trimming A by 20 170 20 205, reference by 239 20 185 20 Trimming A by 20 170 20 205, reference by 22 170 20 205 Trimming A by 20 170 20 205, reference by 21 150 165 25 Disparity in stage position after aligning to center of map frame: -0.20 -0.05 Trimming A by 22 140 20 110, reference by 22 170 20 205 Disparity in stage position after aligning to target position: -0.19 0.20 (2nd move error 0.00 0.23) Finished aligning to item in 2 rounds Trimming A by 21 140 75 100, reference by 22 140 20 110 -1.0 3.3 unbinned pixels; 13.6 1.4 nm along two shift axes; -12.8 -4.6 nm on specimen axes Total_Shift = 6.969408 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.41 microns changed by -2.71 to target drift = 0.08 nm/sec Measured defocus = -1.34 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4754.tif 27 frames are being saved to U:\krios\20190717\PilA_4755.tif 27 frames are being saved to U:\krios\20190717\PilA_4756.tif 27 frames are being saved to U:\krios\20190717\PilA_4757.tif 27 frames are being saved to U:\krios\20190717\PilA_4758.tif 27 frames are being saved to U:\krios\20190717\PilA_4759.tif Running script 17 at item 897 (-233.67, -33.40) Remaining time to fill dewars is 4620 Dewars are NOT busy filling Trimming A by 235 23 20 235, reference by 231 22 20 240 Trimming A by 235 23 20 235, reference by 239 20 185 20 Trimming A by 235 23 20 235, reference by 22 170 20 205 Trimming A by 235 23 20 235, reference by 21 150 165 25 Disparity in stage position after aligning to center of map frame: -0.16 -0.06 Trimming A by 52 22 20 100, reference by 231 22 20 240 Disparity in stage position after aligning to target position: 0.01 0.12 (2nd move error 0.16 0.16) Finished aligning to item in 2 rounds Trimming A by 66 20 20 90, reference by 52 22 20 100 -1.9 -0.1 unbinned pixels; 2.2 7.0 nm along two shift axes; 0.8 -7.4 nm on specimen axes Total_Shift = 3.820958 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.38 microns changed by -2.78 to target drift = 0.16 nm/sec Measured defocus = -1.45 microns changed by 0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4760.tif 27 frames are being saved to U:\krios\20190717\PilA_4761.tif 27 frames are being saved to U:\krios\20190717\PilA_4762.tif 27 frames are being saved to U:\krios\20190717\PilA_4763.tif 27 frames are being saved to U:\krios\20190717\PilA_4764.tif 27 frames are being saved to U:\krios\20190717\PilA_4765.tif Running script 17 at item 898 (-234.94, -35.97) Remaining time to fill dewars is 4440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 37 21 20 105, reference by 22 170 20 205 Disparity in stage position after aligning to target position: -0.21 -0.01 (2nd move error -0.01 0.02) Finished aligning to item in 1 rounds Trimming A by 37 21 20 105, reference by 37 21 20 105 -0.9 3.1 unbinned pixels; 12.4 1.3 nm along two shift axes; -11.7 -4.2 nm on specimen axes Total_Shift = 6.376115 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.19 microns changed by -2.69 to target drift = 0.12 nm/sec Measured defocus = -1.46 microns changed by -0.04 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4766.tif 27 frames are being saved to U:\krios\20190717\PilA_4767.tif 27 frames are being saved to U:\krios\20190717\PilA_4768.tif 27 frames are being saved to U:\krios\20190717\PilA_4769.tif 27 frames are being saved to U:\krios\20190717\PilA_4770.tif 27 frames are being saved to U:\krios\20190717\PilA_4771.tif Running script 17 at item 899 (-236.64, -38.61) Remaining time to fill dewars is 4200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 105 20 150, reference by 22 170 20 205 Disparity in stage position after aligning to target position: -0.26 -0.07 (2nd move error -0.06 -0.04) Finished aligning to item in 1 rounds Trimming A by 21 105 20 150, reference by 21 105 20 150 -5.4 12.7 unbinned pixels; 53.8 0.3 nm along two shift axes; -48.3 -23.7 nm on specimen axes Total_Shift = 27.607874 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.15 microns changed by -2.75 to target drift = 0.07 nm/sec Measured defocus = -1.63 microns changed by 0.03 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4772.tif 27 frames are being saved to U:\krios\20190717\PilA_4773.tif 27 frames are being saved to U:\krios\20190717\PilA_4774.tif 27 frames are being saved to U:\krios\20190717\PilA_4775.tif 27 frames are being saved to U:\krios\20190717\PilA_4776.tif 27 frames are being saved to U:\krios\20190717\PilA_4777.tif Running script 17 at item 900 (76.59, 6.71) Remaining time to fill dewars is 4080 Dewars are NOT busy filling Trimming A by 21 165 160 20, reference by 295 23 20 290 Trimming A by 21 165 160 20, reference by 290 24 180 20 Trimming A by 21 165 160 20, reference by 20 175 20 265 Trimming A by 21 165 160 20, reference by 24 152 165 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.10 Trimming A by 24 95 90 20, reference by 24 152 165 20 Disparity in stage position after aligning to target position: -0.20 -0.20 (2nd move error 0.03 -0.13) Finished aligning to item in 2 rounds Trimming A by 22 82 105 20, reference by 24 95 90 20 -16.5 0.2 unbinned pixels; 22.1 59.0 nm along two shift axes; 2.8 -64.1 nm on specimen axes Total_Shift = 32.938722 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.60 microns changed by -2.30 to target drift = 0.20 nm/sec Measured defocus = -3.05 microns changed by 1.35 to target drift = 0.28 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4778.tif 27 frames are being saved to U:\krios\20190717\PilA_4779.tif 27 frames are being saved to U:\krios\20190717\PilA_4780.tif 27 frames are being saved to U:\krios\20190717\PilA_4781.tif 27 frames are being saved to U:\krios\20190717\PilA_4782.tif 27 frames are being saved to U:\krios\20190717\PilA_4783.tif Running script 17 at item 901 (74.95, 4.11) Remaining time to fill dewars is 3900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 130 65 60, reference by 24 152 165 20 Disparity in stage position after aligning to target position: -0.20 -0.20 (2nd move error 0.03 -0.13) Finished aligning to item in 1 rounds Trimming A by 20 130 70 65, reference by 20 130 65 60 -15.9 -1.5 unbinned pixels; 15.1 59.7 nm along two shift axes; 9.4 -61.7 nm on specimen axes Total_Shift = 32.043497 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.00 microns changed by -1.80 to target drift = 0.00 nm/sec Using peak at -13.8,-13.8, rejecting 2.07 times higher peak at -0.0,-0.0 Using peak at 18.4,7.3, rejecting 2.42 times higher peak at 0.0,0.0 Measured defocus = -0.93 microns changed by -0.87 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4784.tif 27 frames are being saved to U:\krios\20190717\PilA_4785.tif 27 frames are being saved to U:\krios\20190717\PilA_4786.tif 27 frames are being saved to U:\krios\20190717\PilA_4787.tif 27 frames are being saved to U:\krios\20190717\PilA_4788.tif 27 frames are being saved to U:\krios\20190717\PilA_4789.tif Running script 17 at item 902 (-774.48, -14.23) Remaining time to fill dewars is 3660 Dewars are NOT busy filling Trimming A by 245 21 180 20, reference by 245 22 20 265 Trimming A by 245 21 180 20, reference by 234 20 180 20 Trimming A by 245 21 180 20, reference by 22 215 50 245 Trimming A by 245 21 180 20, reference by 23 182 180 20 Disparity in stage position after aligning to center of map frame: -0.17 -0.16 Trimming A by 100 60 180 20, reference by 234 20 180 20 Disparity in stage position after aligning to target position: -0.04 -0.09 (2nd move error 0.12 0.06) Finished aligning to item in 2 rounds Trimming A by 96 49 190 20, reference by 100 60 180 20 -1.2 -0.5 unbinned pixels; 0.4 5.0 nm along two shift axes; 2.3 -4.5 nm on specimen axes Total_Shift = 2.58642 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.18 microns changed by -3.08 to target drift = 0.19 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4790.tif 27 frames are being saved to U:\krios\20190717\PilA_4791.tif 27 frames are being saved to U:\krios\20190717\PilA_4792.tif 27 frames are being saved to U:\krios\20190717\PilA_4793.tif 27 frames are being saved to U:\krios\20190717\PilA_4794.tif 27 frames are being saved to U:\krios\20190717\PilA_4795.tif Running script 17 at item 903 (-777.03, -12.52) Remaining time to fill dewars is 3480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 125 24 130 20, reference by 234 20 180 20 Disparity in stage position after aligning to target position: -0.07 -0.17 (2nd move error 0.08 -0.02) Finished aligning to item in 1 rounds Trimming A by 125 20 135 20, reference by 125 24 130 20 -4.1 1.4 unbinned pixels; 10.5 12.7 nm along two shift axes; -4.5 -16.3 nm on specimen axes Total_Shift = 8.678515 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = -0.15 microns changed by -1.85 to target drift = 0.09 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4796.tif 27 frames are being saved to U:\krios\20190717\PilA_4797.tif 27 frames are being saved to U:\krios\20190717\PilA_4798.tif 27 frames are being saved to U:\krios\20190717\PilA_4799.tif 27 frames are being saved to U:\krios\20190717\PilA_4800.tif 27 frames are being saved to U:\krios\20190717\PilA_4801.tif Running script 17 at item 904 (-779.54, -10.84) Remaining time to fill dewars is 3360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 21 105 20, reference by 234 20 180 20 Disparity in stage position after aligning to target position: -0.09 -0.24 (2nd move error 0.07 -0.10) Finished aligning to item in 1 rounds Trimming A by 140 21 105 20, reference by 140 21 105 20 -10.7 -1.4 unbinned pixels; 9.0 40.7 nm along two shift axes; 7.6 -41.5 nm on specimen axes Total_Shift = 21.654887 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.26 microns changed by -3.36 to target drift = 0.07 nm/sec Measured defocus = -2.17 microns changed by 0.07 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4802.tif 27 frames are being saved to U:\krios\20190717\PilA_4803.tif 27 frames are being saved to U:\krios\20190717\PilA_4804.tif 27 frames are being saved to U:\krios\20190717\PilA_4805.tif 27 frames are being saved to U:\krios\20190717\PilA_4806.tif 27 frames are being saved to U:\krios\20190717\PilA_4807.tif Running script 17 at item 905 (-782.07, -9.13) Remaining time to fill dewars is 3060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 170 20 65 85, reference by 234 20 180 20 Disparity in stage position after aligning to target position: -0.13 -0.30 (2nd move error 0.03 -0.16) Finished aligning to item in 1 rounds Trimming A by 170 20 60 80, reference by 170 20 65 85 -21.5 -1.7 unbinned pixels; 21.8 79.8 nm along two shift axes; 11.1 -83.1 nm on specimen axes Total_Shift = 43.051166 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.09 microns changed by -2.11 to target drift = 0.12 nm/sec Measured defocus = -2.26 microns changed by 0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4808.tif 27 frames are being saved to U:\krios\20190717\PilA_4809.tif 27 frames are being saved to U:\krios\20190717\PilA_4810.tif 27 frames are being saved to U:\krios\20190717\PilA_4811.tif 27 frames are being saved to U:\krios\20190717\PilA_4812.tif 27 frames are being saved to U:\krios\20190717\PilA_4813.tif Running script 17 at item 906 (-783.73, -12.04) Remaining time to fill dewars is 2940 Dewars are NOT busy filling Trimming A by 250 20 20 260, reference by 245 22 20 265 Trimming A by 250 20 20 260, reference by 234 20 180 20 Trimming A by 250 20 20 260, reference by 22 215 50 245 Trimming A by 250 20 20 260, reference by 23 182 180 20 Disparity in stage position after aligning to center of map frame: -0.13 -0.07 Trimming A by 115 24 20 100, reference by 245 22 20 265 Disparity in stage position after aligning to target position: -0.00 0.11 (2nd move error 0.12 0.17) Finished aligning to item in 2 rounds Trimming A by 120 23 20 95, reference by 115 24 20 100 -1.6 -0.9 unbinned pixels; 1.3 7.2 nm along two shift axes; 3.9 -6.1 nm on specimen axes Total_Shift = 3.69521 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.10 microns changed by -3.40 to target drift = 0.16 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4814.tif 27 frames are being saved to U:\krios\20190717\PilA_4815.tif 27 frames are being saved to U:\krios\20190717\PilA_4816.tif 27 frames are being saved to U:\krios\20190717\PilA_4817.tif 27 frames are being saved to U:\krios\20190717\PilA_4818.tif 27 frames are being saved to U:\krios\20190717\PilA_4819.tif Running script 17 at item 907 (-781.11, -13.38) Remaining time to fill dewars is 2760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 20 45 20, reference by 234 20 180 20 Disparity in stage position after aligning to target position: -0.16 -0.23 (2nd move error -0.00 -0.09) Finished aligning to item in 1 rounds Trimming A by 65 20 45 20, reference by 65 20 45 20 -12.2 2.3 unbinned pixels; 24.3 40.6 nm along two shift axes; -6.3 -48.0 nm on specimen axes Total_Shift = 24.86776 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.40 microns changed by -2.00 to target drift = 0.07 nm/sec Measured defocus = -2.43 microns changed by 0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4820.tif 27 frames are being saved to U:\krios\20190717\PilA_4821.tif 27 frames are being saved to U:\krios\20190717\PilA_4822.tif 27 frames are being saved to U:\krios\20190717\PilA_4823.tif 27 frames are being saved to U:\krios\20190717\PilA_4824.tif 27 frames are being saved to U:\krios\20190717\PilA_4825.tif Running script 17 at item 908 (-778.52, -15.02) Remaining time to fill dewars is 2700 Dewars are NOT busy filling Trimming A by 24 195 170 20, reference by 245 22 20 265 Trimming A by 24 195 170 20, reference by 234 20 180 20 Trimming A by 24 195 170 20, reference by 22 215 50 245 Trimming A by 24 195 170 20, reference by 23 182 180 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.14 Trimming A by 55 36 75 20, reference by 23 182 180 20 Disparity in stage position after aligning to target position: -0.21 -0.29 (2nd move error -0.11 -0.17) Finished aligning to item in 2 rounds Trimming A by 55 41 70 20, reference by 55 36 75 20 -17.5 21.6 unbinned pixels; 102.1 30.4 nm along two shift axes; -80.2 -72.5 nm on specimen axes Total_Shift = 55.520529 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.77 microns changed by -3.27 to target drift = 0.04 nm/sec Measured defocus = -2.58 microns changed by 0.08 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4826.tif 27 frames are being saved to U:\krios\20190717\PilA_4827.tif 27 frames are being saved to U:\krios\20190717\PilA_4828.tif 27 frames are being saved to U:\krios\20190717\PilA_4829.tif 27 frames are being saved to U:\krios\20190717\PilA_4830.tif 27 frames are being saved to U:\krios\20190717\PilA_4831.tif Running script 17 at item 909 (-775.98, -16.77) Remaining time to fill dewars is 2460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 78 165 20, reference by 23 182 180 20 Disparity in stage position after aligning to target position: -0.16 -0.15 (2nd move error -0.06 -0.04) Finished aligning to item in 1 rounds Trimming A by 24 82 160 20, reference by 23 78 165 20 -4.4 13.0 unbinned pixels; 53.5 3.5 nm along two shift axes; -49.5 -20.0 nm on specimen axes Total_Shift = 27.417974 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.86 microns changed by -1.74 to target drift = 0.06 nm/sec Measured defocus = -2.59 microns changed by -0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4832.tif 27 frames are being saved to U:\krios\20190717\PilA_4833.tif 27 frames are being saved to U:\krios\20190717\PilA_4834.tif 27 frames are being saved to U:\krios\20190717\PilA_4835.tif 27 frames are being saved to U:\krios\20190717\PilA_4836.tif 27 frames are being saved to U:\krios\20190717\PilA_4837.tif Running script 17 at item 910 (-777.60, -19.41) Remaining time to fill dewars is 2220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 135 120 20, reference by 23 182 180 20 Disparity in stage position after aligning to target position: -0.10 -0.25 (2nd move error -0.00 -0.13) Finished aligning to item in 1 rounds Trimming A by 22 135 120 20, reference by 22 135 120 20 -16.0 2.0 unbinned pixels; 28.3 54.6 nm along two shift axes; -4.5 -62.7 nm on specimen axes Total_Shift = 32.258669 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.41 microns changed by -3.31 to target drift = 0.02 nm/sec Measured defocus = -0.93 microns changed by 0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4838.tif 27 frames are being saved to U:\krios\20190717\PilA_4839.tif 27 frames are being saved to U:\krios\20190717\PilA_4840.tif 27 frames are being saved to U:\krios\20190717\PilA_4841.tif 27 frames are being saved to U:\krios\20190717\PilA_4842.tif 27 frames are being saved to U:\krios\20190717\PilA_4843.tif Running script 17 at item 911 (-780.11, -17.65) Remaining time to fill dewars is 2040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 65 50 20, reference by 23 182 180 20 Disparity in stage position after aligning to target position: -0.14 -0.32 (2nd move error -0.04 -0.20) Finished aligning to item in 1 rounds Trimming A by 24 70 50 20, reference by 24 65 50 20 -23.7 9.0 unbinned pixels; 64.2 71.7 nm along two shift axes; -30.2 -94.1 nm on specimen axes Total_Shift = 50.710457 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.44 microns changed by -3.44 to target drift = 0.04 nm/sec Measured defocus = -1.08 microns changed by 0.08 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4844.tif 27 frames are being saved to U:\krios\20190717\PilA_4845.tif 27 frames are being saved to U:\krios\20190717\PilA_4846.tif 27 frames are being saved to U:\krios\20190717\PilA_4847.tif 27 frames are being saved to U:\krios\20190717\PilA_4848.tif 27 frames are being saved to U:\krios\20190717\PilA_4849.tif Running script 17 at item 912 (-782.66, -15.88) Remaining time to fill dewars is 1920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 35 20 50, reference by 23 182 180 20 Disparity in stage position after aligning to target position: -0.18 -0.39 (2nd move error -0.08 -0.28) Finished aligning to item in 1 rounds Trimming A by 21 35 20 55, reference by 20 35 20 50 -24.2 15.9 unbinned pixels; 89.9 63.2 nm along two shift axes; -56.6 -97.4 nm on specimen axes Total_Shift = 57.846143 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.43 microns changed by -3.53 to target drift = 0.04 nm/sec Measured defocus = -1.19 microns changed by 0.09 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4850.tif 27 frames are being saved to U:\krios\20190717\PilA_4851.tif 27 frames are being saved to U:\krios\20190717\PilA_4852.tif 27 frames are being saved to U:\krios\20190717\PilA_4853.tif 27 frames are being saved to U:\krios\20190717\PilA_4854.tif 27 frames are being saved to U:\krios\20190717\PilA_4855.tif Running script 17 at item 913 (-785.38, -14.66) Remaining time to fill dewars is 1680 Dewars are NOT busy filling Trimming A by 241 22 20 270, reference by 245 22 20 265 Trimming A by 241 22 20 270, reference by 234 20 180 20 Trimming A by 241 22 20 270, reference by 22 215 50 245 Trimming A by 241 22 20 270, reference by 23 182 180 20 Disparity in stage position after aligning to center of map frame: 0.06 0.06 Trimming A by 61 23 20 135, reference by 245 22 20 265 Disparity in stage position after aligning to target position: 0.19 0.02 (2nd move error 0.12 -0.07) Finished aligning to item in 2 rounds Trimming A by 61 23 20 130, reference by 61 23 20 135 -8.5 2.6 unbinned pixels; 20.8 26.5 nm along two shift axes; -8.5 -33.5 nm on specimen axes Total_Shift = 17.731412 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.24 microns changed by -3.44 to target drift = 0.08 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4856.tif 27 frames are being saved to U:\krios\20190717\PilA_4857.tif 27 frames are being saved to U:\krios\20190717\PilA_4858.tif 27 frames are being saved to U:\krios\20190717\PilA_4859.tif 27 frames are being saved to U:\krios\20190717\PilA_4860.tif 27 frames are being saved to U:\krios\20190717\PilA_4861.tif Running script 17 at item 914 (-786.76, -17.32) Remaining time to fill dewars is 1620 Dewars are NOT busy filling Trimming A by 22 215 50 245, reference by 245 22 20 265 Trimming A by 22 215 50 245, reference by 234 20 180 20 Trimming A by 22 215 50 245, reference by 22 215 50 245 Trimming A by 22 215 50 245, reference by 23 182 180 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.12 Trimming A by 21 77 20 165, reference by 22 215 50 245 Disparity in stage position after aligning to target position: -0.20 -0.00 (2nd move error -0.15 0.10) Finished aligning to item in 2 rounds Trimming A by 30 90 20 150, reference by 21 77 20 165 -1.6 31.7 unbinned pixels; 118.6 41.8 nm along two shift axes; -122.9 -13.1 nm on specimen axes Total_Shift = 63.445465 Trimming A by 23 81 20 160, reference by 21 77 20 165 0.1 21.5 unbinned pixels; 78.9 32.6 nm along two shift axes; -83.6 -4.2 nm on specimen axes Total_Shift = 42.974545 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.29 microns changed by -3.59 to target drift = 0.17 nm/sec Measured defocus = -1.33 microns changed by 0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4862.tif 27 frames are being saved to U:\krios\20190717\PilA_4863.tif 27 frames are being saved to U:\krios\20190717\PilA_4864.tif 27 frames are being saved to U:\krios\20190717\PilA_4865.tif 27 frames are being saved to U:\krios\20190717\PilA_4866.tif 27 frames are being saved to U:\krios\20190717\PilA_4867.tif Running script 17 at item 915 (-784.19, -19.02) Remaining time to fill dewars is 1260 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 20 110 20 120, reference by 22 215 50 245 Disparity in stage position after aligning to target position: -0.17 0.02 (2nd move error -0.12 0.12) Finished aligning to item in 1 rounds Trimming A by 20 110 20 115, reference by 20 110 20 120 2.9 21.9 unbinned pixels; 76.7 43.2 nm along two shift axes; -85.7 6.5 nm on specimen axes Total_Shift = 44.104569 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.07 microns changed by -3.47 to target drift = 0.19 nm/sec Measured defocus = -1.41 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4868.tif 27 frames are being saved to U:\krios\20190717\PilA_4869.tif 27 frames are being saved to U:\krios\20190717\PilA_4870.tif 27 frames are being saved to U:\krios\20190717\PilA_4871.tif 27 frames are being saved to U:\krios\20190717\PilA_4872.tif 27 frames are being saved to U:\krios\20190717\PilA_4873.tif Running script 17 at item 916 (-781.73, -20.25) Remaining time to fill dewars is 1140 Dewars are NOT busy filling Trimming A by 24 195 170 20, reference by 245 22 20 265 Trimming A by 24 195 170 20, reference by 234 20 180 20 Trimming A by 24 195 170 20, reference by 22 215 50 245 Trimming A by 24 195 170 20, reference by 23 182 180 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.12 Trimming A by 23 125 20 90, reference by 23 182 180 20 Disparity in stage position after aligning to target position: -0.13 -0.34 (2nd move error -0.05 -0.25) Finished aligning to item in 2 rounds Trimming A by 21 130 65 90, reference by 23 125 20 90 -25.3 10.7 unbinned pixels; 72.3 74.9 nm along two shift axes; -36.3 -100.6 nm on specimen axes Total_Shift = 54.878519 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.88 microns changed by -3.38 to target drift = 0.05 nm/sec Measured defocus = -1.51 microns changed by 0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4874.tif 27 frames are being saved to U:\krios\20190717\PilA_4875.tif 27 frames are being saved to U:\krios\20190717\PilA_4876.tif 27 frames are being saved to U:\krios\20190717\PilA_4877.tif 27 frames are being saved to U:\krios\20190717\PilA_4878.tif 27 frames are being saved to U:\krios\20190717\PilA_4879.tif Running script 17 at item 917 (-64.62, -123.66) Remaining time to fill dewars is 900 Dewars are NOT busy filling Trimming A by 290 23 215 20, reference by 295 23 20 280 Trimming A by 290 23 215 20, reference by 285 20 210 20 Trimming A by 290 23 215 20, reference by 23 180 20 255 Trimming A by 290 23 215 20, reference by 20 210 205 20 Disparity in stage position after aligning to center of map frame: -0.15 -0.11 Trimming A by 150 24 170 20, reference by 285 20 210 20 Disparity in stage position after aligning to target position: -0.12 -0.20 (2nd move error 0.02 -0.10) Finished aligning to item in 2 rounds Trimming A by 150 24 170 20, reference by 150 24 170 20 -16.8 -2.1 unbinned pixels; 14.3 63.8 nm along two shift axes; 11.7 -65.1 nm on specimen axes Total_Shift = 33.930205 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.77 microns changed by -2.37 to target drift = 0.13 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4880.tif 27 frames are being saved to U:\krios\20190717\PilA_4881.tif 27 frames are being saved to U:\krios\20190717\PilA_4882.tif 27 frames are being saved to U:\krios\20190717\PilA_4883.tif 27 frames are being saved to U:\krios\20190717\PilA_4884.tif 27 frames are being saved to U:\krios\20190717\PilA_4885.tif Running script 17 at item 918 (-66.09, -126.11) Remaining time to fill dewars is 720 Dewars are NOT busy filling Trimming A by 20 197 220 20, reference by 295 23 20 280 Trimming A by 20 197 220 20, reference by 285 20 210 20 Trimming A by 20 197 220 20, reference by 23 180 20 255 Trimming A by 20 197 220 20, reference by 20 210 205 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.20 Trimming A by 80 60 105 20, reference by 20 210 205 20 Disparity in stage position after aligning to target position: -0.15 -0.32 (2nd move error -0.02 -0.13) Finished aligning to item in 2 rounds Trimming A by 80 60 105 20, reference by 80 60 105 20 -19.7 5.3 unbinned pixels; 45.2 63.0 nm along two shift axes; -16.5 -77.8 nm on specimen axes Total_Shift = 40.808629 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.60 microns changed by -2.30 to target drift = 0.01 nm/sec Measured defocus = -1.71 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4886.tif 27 frames are being saved to U:\krios\20190717\PilA_4887.tif 27 frames are being saved to U:\krios\20190717\PilA_4888.tif 27 frames are being saved to U:\krios\20190717\PilA_4889.tif 27 frames are being saved to U:\krios\20190717\PilA_4890.tif 27 frames are being saved to U:\krios\20190717\PilA_4891.tif Running script 17 at item 919 (-71.17, -123.14) Remaining time to fill dewars is 600 Dewars are NOT busy filling Trimming A by 295 23 20 280, reference by 295 23 20 280 Trimming A by 295 23 20 280, reference by 285 20 210 20 Trimming A by 295 23 20 280, reference by 23 180 20 255 Trimming A by 295 23 20 280, reference by 20 210 205 20 Disparity in stage position after aligning to center of map frame: -0.02 -0.00 Trimming A by 150 22 20 130, reference by 295 23 20 280 Disparity in stage position after aligning to target position: 0.15 0.02 (2nd move error 0.16 0.00) Finished aligning to item in 2 rounds Trimming A by 150 21 20 125, reference by 150 22 20 130 0.8 1.3 unbinned pixels; 3.9 4.9 nm along two shift axes; -5.3 2.8 nm on specimen axes Total_Shift = 3.099982 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.48 microns changed by -2.28 to target drift = 0.11 nm/sec Measured defocus = -1.80 microns changed by -0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4892.tif 27 frames are being saved to U:\krios\20190717\PilA_4893.tif 27 frames are being saved to U:\krios\20190717\PilA_4894.tif 27 frames are being saved to U:\krios\20190717\PilA_4895.tif 27 frames are being saved to U:\krios\20190717\PilA_4896.tif 27 frames are being saved to U:\krios\20190717\PilA_4897.tif Running script 17 at item 920 (-72.81, -125.86) Remaining time to fill dewars is 420 Dewars are NOT busy filling Trimming A by 21 180 20 250, reference by 295 23 20 280 Trimming A by 21 180 20 250, reference by 285 20 210 20 Trimming A by 21 180 20 250, reference by 23 180 20 255 Trimming A by 21 180 20 250, reference by 20 210 205 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.13 Trimming A by 86 21 20 165, reference by 23 180 20 255 Disparity in stage position after aligning to target position: 0.84 1.18 (2nd move error 0.91 1.29) Finished aligning to item in 2 rounds Trimming A by 125 20 20 135, reference by 86 21 20 165 4.7 -8.5 unbinned pixels; 37.4 4.3 nm along two shift axes; 32.0 20.3 nm on specimen axes Total_Shift = 19.441879 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.40 microns changed by -2.30 to target drift = 0.19 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4898.tif 27 frames are being saved to U:\krios\20190717\PilA_4899.tif 27 frames are being saved to U:\krios\20190717\PilA_4900.tif 27 frames are being saved to U:\krios\20190717\PilA_4901.tif 27 frames are being saved to U:\krios\20190717\PilA_4902.tif 27 frames are being saved to U:\krios\20190717\PilA_4903.tif Running script 17 at item 921 (-74.36, -128.40) Remaining time to fill dewars is 240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 75 20 170, reference by 23 180 20 255 Disparity in stage position after aligning to target position: -0.01 -0.10 (2nd move error 0.05 0.01) Finished aligning to item in 1 rounds Trimming A by 60 80 20 160, reference by 55 75 20 170 0.4 2.1 unbinned pixels; 7.3 4.5 nm along two shift axes; -8.3 1.0 nm on specimen axes Total_Shift = 4.293023 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.24 microns changed by -2.24 to target drift = 0.10 nm/sec Measured defocus = -2.04 microns changed by 0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4904.tif 27 frames are being saved to U:\krios\20190717\PilA_4905.tif 27 frames are being saved to U:\krios\20190717\PilA_4906.tif 27 frames are being saved to U:\krios\20190717\PilA_4907.tif 27 frames are being saved to U:\krios\20190717\PilA_4908.tif 27 frames are being saved to U:\krios\20190717\PilA_4909.tif Running script 17 at item 922 (-71.88, -130.16) Remaining time to fill dewars is 120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 115 20 130, reference by 23 180 20 255 Disparity in stage position after aligning to target position: 0.10 0.01 (2nd move error 0.17 0.13) Finished aligning to item in 1 rounds Trimming A by 24 92 20 145, reference by 20 115 20 130 -0.5 0.8 unbinned pixels; 3.6 0.4 nm along two shift axes; -3.1 -2.0 nm on specimen axes Total_Shift = 1.886236 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Using peak at -92.3,191.0, rejecting 1.25 times higher peak at 0.0,0.3 Using peak at -46.3,33.3, rejecting 1.11 times higher peak at -0.2,-0.0 Measured defocus = 1.02 microns changed by -3.12 to target drift = 2.93 nm/sec Measured defocus = -3.58 microns changed by 1.48 to target drift = 0.24 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4910.tif 27 frames are being saved to U:\krios\20190717\PilA_4911.tif 27 frames are being saved to U:\krios\20190717\PilA_4912.tif 27 frames are being saved to U:\krios\20190717\PilA_4913.tif 27 frames are being saved to U:\krios\20190717\PilA_4914.tif 27 frames are being saved to U:\krios\20190717\PilA_4915.tif Running script 17 at item 923 (-196.71, 22.73) Remaining time to fill dewars is 2580 Dewars ARE busy filling Dewars ARE busy filling Dewars are NOT busy filling Trimming A by 297 22 220 20, reference by 285 23 20 255 Trimming A by 297 22 220 20, reference by 283 20 225 20 Trimming A by 297 22 220 20, reference by 24 180 20 230 Trimming A by 297 22 220 20, reference by 23 180 180 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.14 Trimming A by 120 22 155 20, reference by 283 20 225 20 Disparity in stage position after aligning to target position: -0.00 -0.20 (2nd move error 0.08 -0.08) Finished aligning to item in 2 rounds Trimming A by 120 21 155 20, reference by 120 22 155 20 -10.0 -1.3 unbinned pixels; 8.3 38.1 nm along two shift axes; 7.1 -38.8 nm on specimen axes Total_Shift = 20.232462 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.28 microns changed by -3.48 to target drift = 0.10 nm/sec Measured defocus = -2.25 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4916.tif 27 frames are being saved to U:\krios\20190717\PilA_4917.tif 27 frames are being saved to U:\krios\20190717\PilA_4918.tif 27 frames are being saved to U:\krios\20190717\PilA_4919.tif 27 frames are being saved to U:\krios\20190717\PilA_4920.tif 27 frames are being saved to U:\krios\20190717\PilA_4921.tif Running script 17 at item 924 (-199.21, 24.41) Remaining time to fill dewars is 31440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 20 110 20, reference by 283 20 225 20 Disparity in stage position after aligning to target position: 0.00 -0.28 (2nd move error 0.08 -0.16) Finished aligning to item in 1 rounds Trimming A by 145 20 110 20, reference by 145 20 110 20 -19.6 -1.6 unbinned pixels; 19.8 72.8 nm along two shift axes; 10.2 -75.8 nm on specimen axes Total_Shift = 39.276307 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.17 microns changed by -2.13 to target drift = 0.15 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4922.tif 27 frames are being saved to U:\krios\20190717\PilA_4923.tif 27 frames are being saved to U:\krios\20190717\PilA_4924.tif 27 frames are being saved to U:\krios\20190717\PilA_4925.tif 27 frames are being saved to U:\krios\20190717\PilA_4926.tif 27 frames are being saved to U:\krios\20190717\PilA_4927.tif Running script 17 at item 925 (-201.72, 26.11) Remaining time to fill dewars is 31380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 170 20 75 80, reference by 283 20 225 20 Disparity in stage position after aligning to target position: 0.08 -0.32 (2nd move error 0.16 -0.21) Finished aligning to item in 1 rounds Trimming A by 170 20 75 70, reference by 170 20 75 80 -23.5 -7.6 unbinned pixels; 2.8 95.8 nm along two shift axes; 34.4 -89.6 nm on specimen axes Total_Shift = 49.290267 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.18 microns changed by -3.58 to target drift = 0.16 nm/sec Measured defocus = -2.48 microns changed by 0.08 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4928.tif 27 frames are being saved to U:\krios\20190717\PilA_4929.tif 27 frames are being saved to U:\krios\20190717\PilA_4930.tif 27 frames are being saved to U:\krios\20190717\PilA_4931.tif 27 frames are being saved to U:\krios\20190717\PilA_4932.tif 27 frames are being saved to U:\krios\20190717\PilA_4933.tif Running script 17 at item 926 (-203.40, 23.16) Remaining time to fill dewars is 31260 Dewars are NOT busy filling Trimming A by 284 22 20 260, reference by 285 23 20 255 Trimming A by 284 22 20 260, reference by 283 20 225 20 Trimming A by 284 22 20 260, reference by 24 180 20 230 Trimming A by 284 22 20 260, reference by 23 180 180 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.06 Trimming A by 110 24 20 100, reference by 285 23 20 255 Disparity in stage position after aligning to target position: 0.10 0.10 (2nd move error 0.14 0.14) Finished aligning to item in 2 rounds Trimming A by 110 20 20 100, reference by 110 24 20 100 -0.9 3.6 unbinned pixels; 14.2 2.1 nm along two shift axes; -13.6 -4.2 nm on specimen axes Total_Shift = 7.331198 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.38 microns changed by -2.12 to target drift = 0.16 nm/sec Measured defocus = -2.51 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4934.tif 27 frames are being saved to U:\krios\20190717\PilA_4935.tif 27 frames are being saved to U:\krios\20190717\PilA_4936.tif 27 frames are being saved to U:\krios\20190717\PilA_4937.tif 27 frames are being saved to U:\krios\20190717\PilA_4938.tif 27 frames are being saved to U:\krios\20190717\PilA_4939.tif Running script 17 at item 927 (-200.65, 21.95) Remaining time to fill dewars is 31020 Dewars are NOT busy filling Trimming A by 21 170 190 20, reference by 285 23 20 255 Trimming A by 21 170 190 20, reference by 283 20 225 20 Trimming A by 21 170 190 20, reference by 24 180 20 230 Trimming A by 21 170 190 20, reference by 23 180 180 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.12 Trimming A by 70 20 55 20, reference by 23 180 180 20 Disparity in stage position after aligning to target position: -0.17 -0.38 (2nd move error -0.07 -0.28) Finished aligning to item in 2 rounds Trimming A by 70 24 50 20, reference by 70 20 55 20 -30.7 14.1 unbinned pixels; 92.1 89.4 nm along two shift axes; -48.5 -122.6 nm on specimen axes Total_Shift = 67.681358 Trimming A by 65 24 50 20, reference by 70 20 55 20 -2.3 7.1 unbinned pixels; 29.0 2.4 nm along two shift axes; -27.0 -10.5 nm on specimen axes Total_Shift = 14.879635 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.80 microns changed by -3.40 to target drift = 0.02 nm/sec Measured defocus = -2.64 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4940.tif 27 frames are being saved to U:\krios\20190717\PilA_4941.tif 27 frames are being saved to U:\krios\20190717\PilA_4942.tif 27 frames are being saved to U:\krios\20190717\PilA_4943.tif 27 frames are being saved to U:\krios\20190717\PilA_4944.tif 27 frames are being saved to U:\krios\20190717\PilA_4945.tif Running script 17 at item 928 (-198.10, 20.21) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 45 43 95 20, reference by 23 180 180 20 Disparity in stage position after aligning to target position: -0.06 -0.25 (2nd move error 0.04 -0.14) Finished aligning to item in 1 rounds Trimming A by 42 37 100 20, reference by 45 43 95 20 -18.8 0.3 unbinned pixels; 25.6 67.4 nm along two shift axes; 2.9 -73.4 nm on specimen axes Total_Shift = 37.689112 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.26 microns changed by -2.16 to target drift = 0.12 nm/sec Measured defocus = -0.93 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4946.tif 27 frames are being saved to U:\krios\20190717\PilA_4947.tif 27 frames are being saved to U:\krios\20190717\PilA_4948.tif 27 frames are being saved to U:\krios\20190717\PilA_4949.tif 27 frames are being saved to U:\krios\20190717\PilA_4950.tif 27 frames are being saved to U:\krios\20190717\PilA_4951.tif Running script 17 at item 929 (-199.72, 17.61) Remaining time to fill dewars is 30840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 85 60 20, reference by 23 180 180 20 Disparity in stage position after aligning to target position: -0.10 -0.35 (2nd move error -0.00 -0.25) Finished aligning to item in 1 rounds Trimming A by 23 90 60 20, reference by 24 85 60 20 -28.7 3.3 unbinned pixels; 49.6 98.3 nm along two shift axes; -6.8 -112.3 nm on specimen axes Total_Shift = 57.753715 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.55 microns changed by -3.55 to target drift = 0.07 nm/sec Measured defocus = -1.07 microns changed by 0.07 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4952.tif 27 frames are being saved to U:\krios\20190717\PilA_4953.tif 27 frames are being saved to U:\krios\20190717\PilA_4954.tif 27 frames are being saved to U:\krios\20190717\PilA_4955.tif 27 frames are being saved to U:\krios\20190717\PilA_4956.tif 27 frames are being saved to U:\krios\20190717\PilA_4957.tif Running script 17 at item 930 (-202.15, 18.97) Remaining time to fill dewars is 30720 Dewars are NOT busy filling Trimming A by 23 171 20 235, reference by 285 23 20 255 Trimming A by 23 171 20 235, reference by 283 20 225 20 Trimming A by 23 171 20 235, reference by 24 180 20 230 Trimming A by 23 171 20 235, reference by 23 180 180 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.11 Trimming A by 21 55 20 70, reference by 24 180 20 230 Disparity in stage position after aligning to target position: -0.14 0.16 (2nd move error -0.11 0.24) Finished aligning to item in 2 rounds Trimming A by 20 50 20 60, reference by 21 55 20 70 -16.0 43.7 unbinned pixels; 181.6 8.0 nm along two shift axes; -166.6 -71.6 nm on specimen axes Total_Shift = 93.11184 Trimming A by 21 50 20 65, reference by 21 55 20 70 -5.2 32.1 unbinned pixels; 124.8 29.7 nm along two shift axes; -123.8 -26.9 nm on specimen axes Total_Shift = 65.026725 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.32 microns changed by -3.42 to target drift = 0.13 nm/sec Measured defocus = -1.10 microns changed by -0.00 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4958.tif 27 frames are being saved to U:\krios\20190717\PilA_4959.tif 27 frames are being saved to U:\krios\20190717\PilA_4960.tif 27 frames are being saved to U:\krios\20190717\PilA_4961.tif 27 frames are being saved to U:\krios\20190717\PilA_4962.tif 27 frames are being saved to U:\krios\20190717\PilA_4963.tif Running script 17 at item 931 (-204.58, 20.58) Remaining time to fill dewars is 30540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 51 20 20 125, reference by 24 180 20 230 Disparity in stage position after aligning to target position: -0.09 0.00 (2nd move error -0.06 0.09) Finished aligning to item in 1 rounds Trimming A by 55 23 20 125, reference by 51 20 20 125 1.8 12.2 unbinned pixels; 42.5 24.7 nm along two shift axes; -47.8 4.3 nm on specimen axes Total_Shift = 24.629439 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.22 microns changed by -3.42 to target drift = 0.18 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4964.tif 27 frames are being saved to U:\krios\20190717\PilA_4965.tif 27 frames are being saved to U:\krios\20190717\PilA_4966.tif 27 frames are being saved to U:\krios\20190717\PilA_4967.tif 27 frames are being saved to U:\krios\20190717\PilA_4968.tif 27 frames are being saved to U:\krios\20190717\PilA_4969.tif Running script 17 at item 932 (-206.25, 17.95) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 105 20 160, reference by 24 180 20 230 Disparity in stage position after aligning to target position: -0.15 -0.04 (2nd move error -0.11 0.05) Finished aligning to item in 1 rounds Trimming A by 20 105 20 160, reference by 20 105 20 160 3.7 22.2 unbinned pixels; 76.8 46.7 nm along two shift axes; -87.1 9.6 nm on specimen axes Total_Shift = 45.006915 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.13 microns changed by -3.43 to target drift = 0.19 nm/sec Measured defocus = -1.25 microns changed by -0.05 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4970.tif 27 frames are being saved to U:\krios\20190717\PilA_4971.tif 27 frames are being saved to U:\krios\20190717\PilA_4972.tif 27 frames are being saved to U:\krios\20190717\PilA_4973.tif 27 frames are being saved to U:\krios\20190717\PilA_4974.tif 27 frames are being saved to U:\krios\20190717\PilA_4975.tif Running script 17 at item 933 (420.56, -398.96) Remaining time to fill dewars is 30300 Dewars are NOT busy filling Trimming A by 70 90 120 70, reference by 245 30 20 220 Trimming A by 70 90 120 70, reference by 244 24 180 20 Trimming A by 70 90 120 70, reference by 20 105 35 140 Trimming A by 70 90 120 70, reference by 23 95 115 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.15 Trimming A by 95 21 130 20, reference by 23 95 115 20 Disparity in stage position after aligning to target position: -0.14 -0.05 (2nd move error -0.02 0.07) Finished aligning to item in 2 rounds Trimming A by 95 21 130 20, reference by 95 21 130 20 0.7 5.6 unbinned pixels; 19.5 10.8 nm along two shift axes; -21.8 1.5 nm on specimen axes Total_Shift = 11.201324 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 3.02 microns changed by -4.42 to target drift = 0.31 nm/sec Measured defocus = -1.46 microns changed by 0.06 to target drift = 0.21 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4976.tif 27 frames are being saved to U:\krios\20190717\PilA_4977.tif 27 frames are being saved to U:\krios\20190717\PilA_4978.tif 27 frames are being saved to U:\krios\20190717\PilA_4979.tif 27 frames are being saved to U:\krios\20190717\PilA_4980.tif 27 frames are being saved to U:\krios\20190717\PilA_4981.tif Running script 17 at item 934 (415.38, -395.54) Remaining time to fill dewars is 30240 Dewars are NOT busy filling Trimming A by 255 23 175 20, reference by 245 30 20 220 Trimming A by 255 23 175 20, reference by 244 24 180 20 Trimming A by 255 23 175 20, reference by 20 105 35 140 Trimming A by 255 23 175 20, reference by 23 95 115 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.14 Trimming A by 125 55 105 75, reference by 244 24 180 20 Disparity in stage position after aligning to target position: -0.08 -0.35 (2nd move error -0.02 -0.23) Finished aligning to item in 2 rounds Trimming A by 111 31 115 20, reference by 125 55 105 75 -30.2 6.4 unbinned pixels; 62.8 99.3 nm along two shift axes; -18.3 -118.9 nm on specimen axes Total_Shift = 61.782424 Trimming A by 156 60 40 75, reference by 125 55 105 75 -4.8 0.8 unbinned pixels; 9.3 16.1 nm along two shift axes; -2.2 -19.0 nm on specimen axes Total_Shift = 9.799403 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.25 microns changed by -2.75 to target drift = 0.07 nm/sec Measured defocus = -1.54 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4982.tif 27 frames are being saved to U:\krios\20190717\PilA_4983.tif 27 frames are being saved to U:\krios\20190717\PilA_4984.tif 27 frames are being saved to U:\krios\20190717\PilA_4985.tif 27 frames are being saved to U:\krios\20190717\PilA_4986.tif Ending Navigator acquisition after current item 27 frames are being saved to U:\krios\20190717\PilA_4987.tif Running script 17 at item 935 (412.93, -393.87) Remaining time to fill dewars is 30120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 23 90 30, reference by 244 24 180 20 Disparity in stage position after aligning to target position: -0.13 -0.50 (2nd move error -0.07 -0.38) Finished aligning to item in 1 rounds Trimming A by 140 23 85 25, reference by 145 23 90 30 -35.3 12.7 unbinned pixels; 92.8 107.8 nm along two shift axes; -42.0 -139.9 nm on specimen axes Total_Shift = 74.984439 Trimming A by 135 24 85 30, reference by 145 23 90 30 -6.1 6.6 unbinned pixels; 32.1 12.0 nm along two shift axes; -24.3 -25.0 nm on specimen axes Total_Shift = 17.884544 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.50 microns changed by -4.10 to target drift = 0.10 nm/sec Measured defocus = -1.71 microns changed by 0.11 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4988.tif 27 frames are being saved to U:\krios\20190717\PilA_4989.tif 27 frames are being saved to U:\krios\20190717\PilA_4990.tif 27 frames are being saved to U:\krios\20190717\PilA_4991.tif 27 frames are being saved to U:\krios\20190717\PilA_4992.tif 27 frames are being saved to U:\krios\20190717\PilA_4993.tif Running script 17 at item 936 (410.32, -392.82) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Trimming A by 245 25 20 220, reference by 245 30 20 220 Trimming A by 245 25 20 220, reference by 244 24 180 20 Trimming A by 245 25 20 220, reference by 20 105 35 140 Trimming A by 245 25 20 220, reference by 23 95 115 20 Disparity in stage position after aligning to center of map frame: -0.02 -0.11 Trimming A by 180 24 20 115, reference by 245 30 20 220 Disparity in stage position after aligning to target position: 0.15 0.13 (2nd move error 0.17 0.22) Finished aligning to item in 2 rounds Trimming A by 185 24 20 110, reference by 180 24 20 115 0.9 2.8 unbinned pixels; 9.2 7.5 nm along two shift axes; -11.2 2.9 nm on specimen axes Total_Shift = 5.921776 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.85 microns changed by -2.55 to target drift = 0.13 nm/sec Measured defocus = -1.67 microns changed by -0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_4994.tif 27 frames are being saved to U:\krios\20190717\PilA_4995.tif 27 frames are being saved to U:\krios\20190717\PilA_4996.tif 27 frames are being saved to U:\krios\20190717\PilA_4997.tif 27 frames are being saved to U:\krios\20190717\PilA_4998.tif 27 frames are being saved to U:\krios\20190717\PilA_4999.tif Running script 17 at item 937 (408.70, -395.36) Remaining time to fill dewars is 29820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 24 20 130, reference by 245 30 20 220 Disparity in stage position after aligning to target position: 0.17 0.03 (2nd move error 0.18 0.12) Finished aligning to item in 1 rounds Trimming A by 131 22 20 140, reference by 145 24 20 130 2.4 1.2 unbinned pixels; 1.3 10.4 nm along two shift axes; -5.2 9.0 nm on specimen axes Total_Shift = 5.355036 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 2.02 microns changed by -3.82 to target drift = 0.14 nm/sec Measured defocus = -1.82 microns changed by 0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5000.tif 27 frames are being saved to U:\krios\20190717\PilA_5001.tif 27 frames are being saved to U:\krios\20190717\PilA_5002.tif 27 frames are being saved to U:\krios\20190717\PilA_5003.tif 27 frames are being saved to U:\krios\20190717\PilA_5004.tif 27 frames are being saved to U:\krios\20190717\PilA_5005.tif Running script 17 at item 938 (411.17, -397.06) Remaining time to fill dewars is 29700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 75 20 20 65, reference by 245 30 20 220 Disparity in stage position after aligning to target position: 0.20 0.15 (2nd move error 0.21 0.24) Finished aligning to item in 1 rounds Trimming A by 70 21 20 70, reference by 75 20 20 65 3.2 0.9 unbinned pixels; 0.7 12.8 nm along two shift axes; -4.3 12.1 nm on specimen axes Total_Shift = 6.596732 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.69 microns changed by -2.58 to target drift = 0.16 nm/sec Measured defocus = -1.89 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5006.tif 27 frames are being saved to U:\krios\20190717\PilA_5007.tif 27 frames are being saved to U:\krios\20190717\PilA_5008.tif 27 frames are being saved to U:\krios\20190717\PilA_5009.tif 27 frames are being saved to U:\krios\20190717\PilA_5010.tif 27 frames are being saved to U:\krios\20190717\PilA_5011.tif Running script 17 at item 939 (416.36, -399.74) Remaining time to fill dewars is 29580 Dewars are NOT busy filling Trimming A by 75 93 115 20, reference by 245 30 20 220 Trimming A by 75 93 115 20, reference by 244 24 180 20 Trimming A by 75 93 115 20, reference by 20 105 35 140 Trimming A by 75 93 115 20, reference by 23 95 115 20 Disparity in stage position after aligning to center of map frame: -0.01 -0.15 Trimming A by 45 20 45 20, reference by 23 95 115 20 Disparity in stage position after aligning to target position: -0.11 -0.32 (2nd move error -0.11 -0.19) Finished aligning to item in 2 rounds Trimming A by 40 21 45 20, reference by 45 20 45 20 -23.8 21.0 unbinned pixels; 108.4 54.2 nm along two shift axes; -76.8 -97.2 nm on specimen axes Total_Shift = 63.584237 Trimming A by 40 21 40 20, reference by 45 20 45 20 -5.9 13.0 unbinned pixels; 55.3 1.6 nm along two shift axes; -49.2 -25.6 nm on specimen axes Total_Shift = 28.463957 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 2.24 microns changed by -4.24 to target drift = 0.03 nm/sec Measured defocus = -2.09 microns changed by 0.09 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5012.tif 27 frames are being saved to U:\krios\20190717\PilA_5013.tif 27 frames are being saved to U:\krios\20190717\PilA_5014.tif 27 frames are being saved to U:\krios\20190717\PilA_5015.tif 27 frames are being saved to U:\krios\20190717\PilA_5016.tif 27 frames are being saved to U:\krios\20190717\PilA_5017.tif Running script 17 at item 940 (419.74, -405.87) Remaining time to fill dewars is 29400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 56 96 145 30, reference by 23 95 115 20 Disparity in stage position after aligning to target position: 0.06 -0.12 (2nd move error 0.06 0.02) Finished aligning to item in 1 rounds Trimming A by 47 97 145 20, reference by 56 96 145 30 1.4 -2.7 unbinned pixels; 11.7 0.9 nm along two shift axes; 10.2 5.9 nm on specimen axes Total_Shift = 6.022881 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.85 microns changed by -2.95 to target drift = 0.17 nm/sec Measured defocus = -2.04 microns changed by -0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5018.tif 27 frames are being saved to U:\krios\20190717\PilA_5019.tif 27 frames are being saved to U:\krios\20190717\PilA_5020.tif 27 frames are being saved to U:\krios\20190717\PilA_5021.tif 27 frames are being saved to U:\krios\20190717\PilA_5022.tif 27 frames are being saved to U:\krios\20190717\PilA_5023.tif Running script 17 at item 941 (414.69, -402.34) Remaining time to fill dewars is 29280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 95 115 20 Disparity in stage position after aligning to target position: -0.00 -0.44 (2nd move error -0.00 -0.31) Finished aligning to item in 1 rounds -28.7 2.5 unbinned pixels; 46.6 99.4 nm along two shift axes; -3.7 -112.0 nm on specimen axes Total_Shift = 57.54307 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.63 microns changed by -2.83 to target drift = 0.14 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5024.tif 27 frames are being saved to U:\krios\20190717\PilA_5025.tif 27 frames are being saved to U:\krios\20190717\PilA_5026.tif 27 frames are being saved to U:\krios\20190717\PilA_5027.tif 27 frames are being saved to U:\krios\20190717\PilA_5028.tif 27 frames are being saved to U:\krios\20190717\PilA_5029.tif Running script 17 at item 942 (409.79, -399.59) Remaining time to fill dewars is 29160 Dewars are NOT busy filling Trimming A by 21 105 30 135, reference by 245 30 20 220 Trimming A by 21 105 30 135, reference by 244 24 180 20 Trimming A by 21 105 30 135, reference by 20 105 35 140 Trimming A by 21 105 30 135, reference by 23 95 115 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.15 Trimming A by 46 20 20 75, reference by 20 105 35 140 Disparity in stage position after aligning to target position: 0.02 0.04 (2nd move error -0.00 0.18) Finished aligning to item in 2 rounds Trimming A by 50 23 20 75, reference by 46 20 20 75 -0.4 3.8 unbinned pixels; 14.5 4.4 nm along two shift axes; -14.7 -2.3 nm on specimen axes Total_Shift = 7.62938 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.25 microns changed by -2.55 to target drift = 0.09 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5030.tif 27 frames are being saved to U:\krios\20190717\PilA_5031.tif 27 frames are being saved to U:\krios\20190717\PilA_5032.tif 27 frames are being saved to U:\krios\20190717\PilA_5033.tif 27 frames are being saved to U:\krios\20190717\PilA_5034.tif 27 frames are being saved to U:\krios\20190717\PilA_5035.tif Running script 17 at item 943 (407.01, -397.91) Remaining time to fill dewars is 28980 Dewars are NOT busy filling Trimming A by 245 22 20 225, reference by 245 30 20 220 Trimming A by 245 22 20 225, reference by 244 24 180 20 Trimming A by 245 22 20 225, reference by 20 105 35 140 Trimming A by 245 22 20 225, reference by 23 95 115 20 Disparity in stage position after aligning to center of map frame: 0.07 -0.16 Trimming A by 116 22 20 150, reference by 245 30 20 220 Disparity in stage position after aligning to target position: 0.20 -0.06 (2nd move error 0.13 0.08) Finished aligning to item in 2 rounds Trimming A by 111 24 20 150, reference by 116 22 20 150 0.1 -0.8 unbinned pixels; 3.2 0.9 nm along two shift axes; 3.2 0.6 nm on specimen axes Total_Shift = 1.671673 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.35 microns changed by -3.75 to target drift = 0.15 nm/sec Measured defocus = -2.46 microns changed by 0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5036.tif 27 frames are being saved to U:\krios\20190717\PilA_5037.tif 27 frames are being saved to U:\krios\20190717\PilA_5038.tif 27 frames are being saved to U:\krios\20190717\PilA_5039.tif 27 frames are being saved to U:\krios\20190717\PilA_5040.tif 27 frames are being saved to U:\krios\20190717\PilA_5041.tif Running script 17 at item 944 (405.62, -400.37) Remaining time to fill dewars is 28800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 92 22 20 165, reference by 20 105 35 140 Disparity in stage position after aligning to target position: -0.08 -0.20 (2nd move error -0.10 -0.07) Finished aligning to item in 1 rounds Trimming A by 101 20 40 160, reference by 92 22 20 165 -7.3 20.2 unbinned pixels; 84.0 4.0 nm along two shift axes; -77.2 -32.9 nm on specimen axes Total_Shift = 43.080599 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.15 microns changed by -2.35 to target drift = 0.05 nm/sec Measured defocus = -2.48 microns changed by -0.02 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5042.tif 27 frames are being saved to U:\krios\20190717\PilA_5043.tif 27 frames are being saved to U:\krios\20190717\PilA_5044.tif 27 frames are being saved to U:\krios\20190717\PilA_5045.tif 27 frames are being saved to U:\krios\20190717\PilA_5046.tif 27 frames are being saved to U:\krios\20190717\PilA_5047.tif Running script 17 at item 945 (408.13, -402.19) Remaining time to fill dewars is 28800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 20 20 85, reference by 20 105 35 140 Disparity in stage position after aligning to target position: 0.06 -0.07 (2nd move error 0.04 0.05) Finished aligning to item in 1 rounds Trimming A by 32 22 20 90, reference by 35 20 20 85 -2.2 -1.9 unbinned pixels; 4.2 10.6 nm along two shift axes; 7.9 -8.0 nm on specimen axes Total_Shift = 5.744664 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 1.22 microns changed by -3.82 to target drift = 0.15 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5048.tif 27 frames are being saved to U:\krios\20190717\PilA_5049.tif 27 frames are being saved to U:\krios\20190717\PilA_5050.tif 27 frames are being saved to U:\krios\20190717\PilA_5051.tif 27 frames are being saved to U:\krios\20190717\PilA_5052.tif 27 frames are being saved to U:\krios\20190717\PilA_5053.tif Running script 17 at item 946 (415.54, -406.66) Remaining time to fill dewars is 28560 Dewars are NOT busy filling Trimming A by 75 95 110 20, reference by 245 30 20 220 Trimming A by 75 95 110 20, reference by 244 24 180 20 Trimming A by 75 95 110 20, reference by 20 105 35 140 Trimming A by 75 95 110 20, reference by 23 95 115 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.15 Trimming A by 24 41 65 20, reference by 23 95 115 20 Disparity in stage position after aligning to target position: 0.02 -0.37 (2nd move error 0.00 -0.24) Finished aligning to item in 2 rounds Trimming A by 20 55 55 20, reference by 24 41 65 20 -26.9 1.1 unbinned pixels; 39.2 95.2 nm along two shift axes; 1.4 -104.9 nm on specimen axes Total_Shift = 53.861646 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.03 microns changed by -2.93 to target drift = 0.08 nm/sec Measured defocus = -0.93 microns changed by 0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5054.tif 27 frames are being saved to U:\krios\20190717\PilA_5055.tif 27 frames are being saved to U:\krios\20190717\PilA_5056.tif 27 frames are being saved to U:\krios\20190717\PilA_5057.tif 27 frames are being saved to U:\krios\20190717\PilA_5058.tif 27 frames are being saved to U:\krios\20190717\PilA_5059.tif Running script 17 at item 947 (418.06, -408.32) Remaining time to fill dewars is 28440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 75 112 130 20, reference by 23 95 115 20 Disparity in stage position after aligning to target position: 0.17 -0.21 (2nd move error 0.15 -0.08) Finished aligning to item in 1 rounds Trimming A by 75 115 135 20, reference by 75 112 130 20 -6.5 3.0 unbinned pixels; 19.6 19.0 nm along two shift axes; -10.3 -26.0 nm on specimen axes Total_Shift = 14.385863 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 3.32 microns changed by -4.32 to target drift = 0.07 nm/sec Measured defocus = -1.02 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5060.tif 27 frames are being saved to U:\krios\20190717\PilA_5061.tif 27 frames are being saved to U:\krios\20190717\PilA_5062.tif 27 frames are being saved to U:\krios\20190717\PilA_5063.tif 27 frames are being saved to U:\krios\20190717\PilA_5064.tif 27 frames are being saved to U:\krios\20190717\PilA_5065.tif Running script 17 at item 948 (416.45, -410.87) Remaining time to fill dewars is 28260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 150 105 20, reference by 23 95 115 20 Disparity in stage position after aligning to target position: 0.17 -0.30 (2nd move error 0.15 -0.18) Finished aligning to item in 1 rounds Trimming A by 23 155 105 20, reference by 23 150 105 20 -19.2 -6.7 unbinned pixels; 0.5 79.2 nm along two shift axes; 30.1 -73.3 nm on specimen axes Total_Shift = 40.660688 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.94 microns changed by -3.04 to target drift = 0.16 nm/sec Measured defocus = -1.15 microns changed by 0.05 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5066.tif 27 frames are being saved to U:\krios\20190717\PilA_5067.tif 27 frames are being saved to U:\krios\20190717\PilA_5068.tif 27 frames are being saved to U:\krios\20190717\PilA_5069.tif 27 frames are being saved to U:\krios\20190717\PilA_5070.tif 27 frames are being saved to U:\krios\20190717\PilA_5071.tif Running script 17 at item 949 (413.88, -409.21) Remaining time to fill dewars is 28140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 105 35 75, reference by 23 95 115 20 Disparity in stage position after aligning to target position: 0.18 -0.48 (2nd move error 0.17 -0.36) Finished aligning to item in 1 rounds Trimming A by 24 105 40 80, reference by 24 105 35 75 -30.7 -6.2 unbinned pixels; 17.2 119.9 nm along two shift axes; 30.7 -118.2 nm on specimen axes Total_Shift = 62.674351 Trimming A by 21 110 70 75, reference by 24 105 35 75 -1.8 -0.7 unbinned pixels; 0.4 7.5 nm along two shift axes; 3.3 -6.8 nm on specimen axes Total_Shift = 3.865406 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 3.04 microns changed by -4.24 to target drift = 0.09 nm/sec Measured defocus = -1.30 microns changed by 0.10 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5072.tif 27 frames are being saved to U:\krios\20190717\PilA_5073.tif 27 frames are being saved to U:\krios\20190717\PilA_5074.tif 27 frames are being saved to U:\krios\20190717\PilA_5075.tif 27 frames are being saved to U:\krios\20190717\PilA_5076.tif 27 frames are being saved to U:\krios\20190717\PilA_5077.tif Running script 17 at item 950 (411.48, -408.12) Remaining time to fill dewars is 27960 Dewars are NOT busy filling Trimming A by 21 91 30 150, reference by 245 30 20 220 Trimming A by 21 91 30 150, reference by 244 24 180 20 Trimming A by 21 91 30 150, reference by 20 105 35 140 Trimming A by 21 91 30 150, reference by 23 95 115 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.16 Trimming A by 23 95 20 95, reference by 20 105 35 140 Disparity in stage position after aligning to target position: 0.15 0.17 (2nd move error 0.10 0.31) Finished aligning to item in 2 rounds Trimming A by 24 90 20 85, reference by 23 95 20 95 -6.0 0.6 unbinned pixels; 10.0 20.8 nm along two shift axes; -0.9 -23.6 nm on specimen axes Total_Shift = 12.101393 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.43 microns changed by -2.73 to target drift = 0.12 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5078.tif 27 frames are being saved to U:\krios\20190717\PilA_5079.tif 27 frames are being saved to U:\krios\20190717\PilA_5080.tif 27 frames are being saved to U:\krios\20190717\PilA_5081.tif 27 frames are being saved to U:\krios\20190717\PilA_5082.tif 27 frames are being saved to U:\krios\20190717\PilA_5083.tif Running script 17 at item 951 (409.01, -406.41) Remaining time to fill dewars is 27840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 105 60 80, reference by 20 105 35 140 Disparity in stage position after aligning to target position: 0.15 -0.04 (2nd move error 0.10 0.10) Finished aligning to item in 1 rounds Trimming A by 70 109 60 70, reference by 70 105 60 80 -0.4 -1.6 unbinned pixels; 5.2 3.8 nm along two shift axes; 6.1 -1.3 nm on specimen axes Total_Shift = 3.22207 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.45 microns changed by -3.85 to target drift = 0.14 nm/sec Measured defocus = -1.39 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5084.tif 27 frames are being saved to U:\krios\20190717\PilA_5085.tif 27 frames are being saved to U:\krios\20190717\PilA_5086.tif 27 frames are being saved to U:\krios\20190717\PilA_5087.tif 27 frames are being saved to U:\krios\20190717\PilA_5088.tif 27 frames are being saved to U:\krios\20190717\PilA_5089.tif Running script 17 at item 952 (406.52, -404.73) Remaining time to fill dewars is 27720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 20 115, reference by 20 105 35 140 Disparity in stage position after aligning to target position: 0.06 -0.17 (2nd move error 0.02 -0.03) Finished aligning to item in 1 rounds Trimming A by 21 80 25 115, reference by 20 80 20 115 -5.5 -1.3 unbinned pixels; 2.3 22.0 nm along two shift axes; 6.4 -21.3 nm on specimen axes Total_Shift = 11.394902 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.97 microns changed by -2.47 to target drift = 0.09 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5090.tif 27 frames are being saved to U:\krios\20190717\PilA_5091.tif 27 frames are being saved to U:\krios\20190717\PilA_5092.tif 27 frames are being saved to U:\krios\20190717\PilA_5093.tif 27 frames are being saved to U:\krios\20190717\PilA_5094.tif 27 frames are being saved to U:\krios\20190717\PilA_5095.tif Running script 17 at item 953 (409.81, -410.68) Remaining time to fill dewars is 27540 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 21 175 20 150, reference by 20 105 35 140 Disparity in stage position after aligning to target position: 0.22 0.06 (2nd move error 0.17 0.20) Finished aligning to item in 1 rounds Trimming A by 20 165 20 145, reference by 21 175 20 150 2.1 0.7 unbinned pixels; 0.3 8.6 nm along two shift axes; -3.0 8.1 nm on specimen axes Total_Shift = 4.426813 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.33 microns changed by -3.93 to target drift = 0.13 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5096.tif 27 frames are being saved to U:\krios\20190717\PilA_5097.tif 27 frames are being saved to U:\krios\20190717\PilA_5098.tif 27 frames are being saved to U:\krios\20190717\PilA_5099.tif 27 frames are being saved to U:\krios\20190717\PilA_5100.tif 27 frames are being saved to U:\krios\20190717\PilA_5101.tif Running script 17 at item 954 (-95.02, -693.24) Remaining time to fill dewars is 27420 Dewars are NOT busy filling Trimming A by 273 24 225 20, reference by 285 21 20 275 Trimming A by 273 24 225 20, reference by 268 21 225 20 Trimming A by 273 24 225 20, reference by 21 207 20 270 Trimming A by 273 24 225 20, reference by 21 225 205 20 Disparity in stage position after aligning to center of map frame: -0.03 -0.12 Trimming A by 120 23 105 20, reference by 268 21 225 20 Disparity in stage position after aligning to target position: -0.05 -0.43 (2nd move error -0.03 -0.33) Finished aligning to item in 2 rounds Trimming A by 115 24 105 20, reference by 120 23 105 20 -25.0 5.4 unbinned pixels; 52.6 81.9 nm along two shift axes; -15.9 -98.5 nm on specimen axes Total_Shift = 51.197791 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = -0.54 microns changed by -1.16 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5102.tif 27 frames are being saved to U:\krios\20190717\PilA_5103.tif 27 frames are being saved to U:\krios\20190717\PilA_5104.tif 27 frames are being saved to U:\krios\20190717\PilA_5105.tif 27 frames are being saved to U:\krios\20190717\PilA_5106.tif 27 frames are being saved to U:\krios\20190717\PilA_5107.tif Running script 17 at item 955 (-97.46, -691.48) Remaining time to fill dewars is 27240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 20 65 80, reference by 268 21 225 20 Disparity in stage position after aligning to target position: -0.05 -0.50 (2nd move error -0.02 -0.40) Finished aligning to item in 1 rounds Trimming A by 140 20 70 85, reference by 150 20 65 80 -38.5 7.5 unbinned pixels; 78.0 127.4 nm along two shift axes; -21.2 -151.5 nm on specimen axes Total_Shift = 78.538223 Trimming A by 140 20 65 80, reference by 150 20 65 80 -5.6 3.4 unbinned pixels; 19.7 15.2 nm along two shift axes; -11.9 -22.5 nm on specimen axes Total_Shift = 13.078673 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.75 microns changed by -2.55 to target drift = 0.06 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5108.tif 27 frames are being saved to U:\krios\20190717\PilA_5109.tif 27 frames are being saved to U:\krios\20190717\PilA_5110.tif 27 frames are being saved to U:\krios\20190717\PilA_5111.tif 27 frames are being saved to U:\krios\20190717\PilA_5112.tif 27 frames are being saved to U:\krios\20190717\PilA_5113.tif Running script 17 at item 956 (-94.05, -697.41) Remaining time to fill dewars is 27120 Dewars are NOT busy filling Trimming A by 21 225 205 20, reference by 285 21 20 275 Trimming A by 21 225 205 20, reference by 268 21 225 20 Trimming A by 21 225 205 20, reference by 21 207 20 270 Trimming A by 21 225 205 20, reference by 21 225 205 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.14 Trimming A by 22 90 120 20, reference by 21 225 205 20 Disparity in stage position after aligning to target position: -0.08 -0.36 (2nd move error 0.00 -0.24) Finished aligning to item in 2 rounds Trimming A by 20 100 115 20, reference by 22 90 120 20 -22.2 2.5 unbinned pixels; 38.0 76.0 nm along two shift axes; -5.0 -86.7 nm on specimen axes Total_Shift = 44.582752 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.65 microns changed by -2.55 to target drift = 0.11 nm/sec Measured defocus = -1.93 microns changed by 0.03 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5114.tif 27 frames are being saved to U:\krios\20190717\PilA_5115.tif 27 frames are being saved to U:\krios\20190717\PilA_5116.tif 27 frames are being saved to U:\krios\20190717\PilA_5117.tif 27 frames are being saved to U:\krios\20190717\PilA_5118.tif 27 frames are being saved to U:\krios\20190717\PilA_5119.tif Running script 17 at item 957 (-98.96, -694.51) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Trimming A by 276 22 20 280, reference by 285 21 20 275 Trimming A by 276 22 20 280, reference by 268 21 225 20 Trimming A by 276 22 20 280, reference by 21 207 20 270 Trimming A by 276 22 20 280, reference by 21 225 205 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.03 Trimming A by 72 22 20 120, reference by 285 21 20 275 Disparity in stage position after aligning to target position: 0.55 0.03 (2nd move error 0.62 0.04) Finished aligning to item in 2 rounds Trimming A by 76 20 20 100, reference by 72 22 20 120 7.0 0.7 unbinned pixels; 6.8 26.3 nm along two shift axes; -4.0 27.2 nm on specimen axes Total_Shift = 14.12794 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Running script 17 at item 958 (-98.11, -698.35) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Trimming A by 20 215 20 260, reference by 285 21 20 275 Trimming A by 20 215 20 260, reference by 268 21 225 20 Trimming A by 20 215 20 260, reference by 21 207 20 270 Trimming A by 20 215 20 260, reference by 21 225 205 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.07 Trimming A by 20 100 20 95, reference by 21 207 20 270 Disparity in stage position after aligning to target position: -0.16 0.24 (2nd move error -0.06 0.29) Finished aligning to item in 2 rounds Trimming A by 20 90 20 95, reference by 20 100 20 95 1.7 14.5 unbinned pixels; 51.1 28.0 nm along two shift axes; -56.7 3.6 nm on specimen axes Total_Shift = 29.18487 Target defocus is: -2.00 um 0.00 degrees Trimming A by 22 122 155 20, reference by 255 24 20 240 Trimming A by 22 122 155 20, reference by 265 20 175 20 Trimming A by 22 122 155 20, reference by 20 145 20 185 Trimming A by 22 122 155 20, reference by 20 130 145 20 Disparity in stage position after aligning to center of map frame: -0.17 -0.16 Trimming A by 80 55 130 20, reference by 20 130 145 20 Disparity in stage position after aligning to target position: -0.17 -0.12 (2nd move error -0.01 0.02) Finished aligning to item in 2 rounds Running script 17 at item 961 (-582.14, -49.46) Remaining time to fill dewars is 26820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 80 55 130 20, reference by 20 130 145 20 Disparity in stage position after aligning to target position: -0.18 -0.09 (2nd move error -0.02 0.06) Finished aligning to item in 1 rounds Trimming A by 85 55 130 20, reference by 80 55 130 20 -2.1 4.8 unbinned pixels; 20.6 0.2 nm along two shift axes; -18.4 -9.2 nm on specimen axes Total_Shift = 10.56601 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.26 microns changed by -3.46 to target drift = 0.17 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5120.tif 27 frames are being saved to U:\krios\20190717\PilA_5121.tif 27 frames are being saved to U:\krios\20190717\PilA_5122.tif 27 frames are being saved to U:\krios\20190717\PilA_5123.tif 27 frames are being saved to U:\krios\20190717\PilA_5124.tif 27 frames are being saved to U:\krios\20190717\PilA_5125.tif Running script 17 at item 962 (-584.72, -47.72) Remaining time to fill dewars is 26700 Dewars are NOT busy filling Trimming A by 270 22 180 20, reference by 255 24 20 240 Trimming A by 270 22 180 20, reference by 265 20 175 20 Trimming A by 270 22 180 20, reference by 20 145 20 185 Trimming A by 270 22 180 20, reference by 20 130 145 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.12 Trimming A by 100 24 105 20, reference by 265 20 175 20 Disparity in stage position after aligning to target position: -0.04 -0.12 (2nd move error 0.06 -0.02) Finished aligning to item in 2 rounds Trimming A by 91 24 115 20, reference by 100 24 105 20 -4.0 -1.3 unbinned pixels; 0.4 16.3 nm along two shift axes; 5.9 -15.2 nm on specimen axes Total_Shift = 8.384791 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.18 microns changed by -2.12 to target drift = 0.10 nm/sec Measured defocus = -2.32 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5126.tif 27 frames are being saved to U:\krios\20190717\PilA_5127.tif 27 frames are being saved to U:\krios\20190717\PilA_5128.tif 27 frames are being saved to U:\krios\20190717\PilA_5129.tif 27 frames are being saved to U:\krios\20190717\PilA_5130.tif 27 frames are being saved to U:\krios\20190717\PilA_5131.tif Running script 17 at item 963 (-587.28, -46.06) Remaining time to fill dewars is 26520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 24 85 20, reference by 265 20 175 20 Disparity in stage position after aligning to target position: -0.06 -0.19 (2nd move error 0.03 -0.09) Finished aligning to item in 1 rounds Trimming A by 115 24 85 20, reference by 115 24 85 20 -11.3 -1.5 unbinned pixels; 9.3 43.1 nm along two shift axes; 8.2 -43.8 nm on specimen axes Total_Shift = 22.872428 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.15 microns changed by -3.55 to target drift = 0.11 nm/sec Measured defocus = -2.44 microns changed by 0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5132.tif 27 frames are being saved to U:\krios\20190717\PilA_5133.tif 27 frames are being saved to U:\krios\20190717\PilA_5134.tif 27 frames are being saved to U:\krios\20190717\PilA_5135.tif 27 frames are being saved to U:\krios\20190717\PilA_5136.tif 27 frames are being saved to U:\krios\20190717\PilA_5137.tif Running script 17 at item 964 (-589.82, -44.34) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 20 55 55, reference by 265 20 175 20 Disparity in stage position after aligning to target position: -0.15 -0.29 (2nd move error -0.05 -0.20) Finished aligning to item in 1 rounds Trimming A by 130 20 70 65, reference by 140 20 55 55 -26.4 11.1 unbinned pixels; 75.3 78.4 nm along two shift axes; -37.6 -105.1 nm on specimen axes Total_Shift = 57.302403 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.15 microns changed by -2.35 to target drift = 0.02 nm/sec Measured defocus = -2.52 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5138.tif 27 frames are being saved to U:\krios\20190717\PilA_5139.tif 27 frames are being saved to U:\krios\20190717\PilA_5140.tif 27 frames are being saved to U:\krios\20190717\PilA_5141.tif 27 frames are being saved to U:\krios\20190717\PilA_5142.tif 27 frames are being saved to U:\krios\20190717\PilA_5143.tif Running script 17 at item 965 (-594.07, -45.61) Remaining time to fill dewars is 26160 Dewars are NOT busy filling Trimming A by 255 24 20 240, reference by 255 24 20 240 Trimming A by 255 24 20 240, reference by 265 20 175 20 Trimming A by 255 24 20 240, reference by 20 145 20 185 Trimming A by 255 24 20 240, reference by 20 130 145 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.09 Trimming A by 142 21 20 165, reference by 255 24 20 240 Disparity in stage position after aligning to target position: 0.14 -0.05 (2nd move error 0.19 0.01) Finished aligning to item in 2 rounds Trimming A by 155 21 20 145, reference by 142 21 20 165 2.5 4.8 unbinned pixels; 14.5 16.1 nm along two shift axes; -19.3 8.6 nm on specimen axes Total_Shift = 10.822926 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 1.19 microns changed by -3.79 to target drift = 0.14 nm/sec Measured defocus = -2.64 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5144.tif 27 frames are being saved to U:\krios\20190717\PilA_5145.tif 27 frames are being saved to U:\krios\20190717\PilA_5146.tif 27 frames are being saved to U:\krios\20190717\PilA_5147.tif 27 frames are being saved to U:\krios\20190717\PilA_5148.tif 27 frames are being saved to U:\krios\20190717\PilA_5149.tif Running script 17 at item 966 (-591.50, -47.33) Remaining time to fill dewars is 25980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 72 22 20 95, reference by 255 24 20 240 Disparity in stage position after aligning to target position: 0.25 -0.06 (2nd move error 0.29 0.01) Finished aligning to item in 1 rounds Trimming A by 85 20 20 75, reference by 72 22 20 95 3.9 -1.4 unbinned pixels; 10.1 12.0 nm along two shift axes; 4.5 15.4 nm on specimen axes Total_Shift = 8.247825 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.54 microns changed by -2.44 to target drift = 0.16 nm/sec Measured defocus = -0.93 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5150.tif 27 frames are being saved to U:\krios\20190717\PilA_5151.tif 27 frames are being saved to U:\krios\20190717\PilA_5152.tif 27 frames are being saved to U:\krios\20190717\PilA_5153.tif 27 frames are being saved to U:\krios\20190717\PilA_5154.tif 27 frames are being saved to U:\krios\20190717\PilA_5155.tif Running script 17 at item 967 (-588.77, -48.49) Remaining time to fill dewars is 25860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 45 20 30 20, reference by 265 20 175 20 Disparity in stage position after aligning to target position: -0.05 -0.21 (2nd move error 0.05 -0.12) Finished aligning to item in 1 rounds Trimming A by 40 20 30 20, reference by 45 20 30 20 -14.2 -0.9 unbinned pixels; 15.3 52.3 nm along two shift axes; 6.4 -54.9 nm on specimen axes Total_Shift = 28.379213 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.67 microns changed by -3.67 to target drift = 0.06 nm/sec Measured defocus = -1.06 microns changed by 0.06 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5156.tif 27 frames are being saved to U:\krios\20190717\PilA_5157.tif 27 frames are being saved to U:\krios\20190717\PilA_5158.tif 27 frames are being saved to U:\krios\20190717\PilA_5159.tif 27 frames are being saved to U:\krios\20190717\PilA_5160.tif 27 frames are being saved to U:\krios\20190717\PilA_5161.tif Running script 17 at item 968 (-586.26, -50.28) Remaining time to fill dewars is 25740 Dewars are NOT busy filling Trimming A by 24 126 150 20, reference by 255 24 20 240 Trimming A by 24 126 150 20, reference by 265 20 175 20 Trimming A by 24 126 150 20, reference by 20 145 20 185 Trimming A by 24 126 150 20, reference by 20 130 145 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.12 Trimming A by 40 20 35 20, reference by 20 130 145 20 Disparity in stage position after aligning to target position: -0.18 -0.19 (2nd move error -0.10 -0.09) Finished aligning to item in 2 rounds Trimming A by 35 21 35 20, reference by 40 20 35 20 -12.2 18.8 unbinned pixels; 85.1 15.4 nm along two shift axes; -70.7 -51.3 nm on specimen axes Total_Shift = 44.846117 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.44 microns changed by -3.54 to target drift = 0.07 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5162.tif 27 frames are being saved to U:\krios\20190717\PilA_5163.tif 27 frames are being saved to U:\krios\20190717\PilA_5164.tif 27 frames are being saved to U:\krios\20190717\PilA_5165.tif 27 frames are being saved to U:\krios\20190717\PilA_5166.tif 27 frames are being saved to U:\krios\20190717\PilA_5167.tif Running script 17 at item 969 (-583.74, -51.98) Remaining time to fill dewars is 25560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 56 120 20, reference by 20 130 145 20 Disparity in stage position after aligning to target position: -0.08 -0.14 (2nd move error 0.01 -0.04) Finished aligning to item in 1 rounds Trimming A by 20 65 115 20, reference by 24 56 120 20 -6.5 -0.3 unbinned pixels; 7.2 23.8 nm along two shift axes; 2.7 -25.0 nm on specimen axes Total_Shift = 12.930481 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.21 microns changed by -3.41 to target drift = 0.06 nm/sec Measured defocus = -1.18 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5168.tif 27 frames are being saved to U:\krios\20190717\PilA_5169.tif 27 frames are being saved to U:\krios\20190717\PilA_5170.tif 27 frames are being saved to U:\krios\20190717\PilA_5171.tif 27 frames are being saved to U:\krios\20190717\PilA_5172.tif 27 frames are being saved to U:\krios\20190717\PilA_5173.tif Running script 17 at item 970 (-585.34, -54.62) Remaining time to fill dewars is 25380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 100 90 20, reference by 20 130 145 20 Disparity in stage position after aligning to target position: -0.04 -0.24 (2nd move error 0.04 -0.14) Finished aligning to item in 1 rounds Trimming A by 21 87 105 20, reference by 22 100 90 20 -16.9 -1.6 unbinned pixels; 16.1 63.2 nm along two shift axes; 9.9 -65.3 nm on specimen axes Total_Shift = 33.907912 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.13 microns changed by -3.43 to target drift = 0.07 nm/sec Measured defocus = -1.28 microns changed by -0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5174.tif 27 frames are being saved to U:\krios\20190717\PilA_5175.tif 27 frames are being saved to U:\krios\20190717\PilA_5176.tif 27 frames are being saved to U:\krios\20190717\PilA_5177.tif 27 frames are being saved to U:\krios\20190717\PilA_5178.tif 27 frames are being saved to U:\krios\20190717\PilA_5179.tif Running script 17 at item 971 (-587.93, -52.95) Remaining time to fill dewars is 25320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 30 25 20, reference by 20 130 145 20 Disparity in stage position after aligning to target position: -0.08 -0.31 (2nd move error 0.00 -0.21) Finished aligning to item in 1 rounds Trimming A by 20 30 25 20, reference by 20 30 25 20 -28.7 2.8 unbinned pixels; 47.9 99.1 nm along two shift axes; -5.0 -112.3 nm on specimen axes Total_Shift = 57.706891 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.21 microns changed by -3.61 to target drift = 0.08 nm/sec Measured defocus = -1.45 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5180.tif 27 frames are being saved to U:\krios\20190717\PilA_5181.tif 27 frames are being saved to U:\krios\20190717\PilA_5182.tif 27 frames are being saved to U:\krios\20190717\PilA_5183.tif 27 frames are being saved to U:\krios\20190717\PilA_5184.tif 27 frames are being saved to U:\krios\20190717\PilA_5185.tif Running script 17 at item 972 (-590.49, -51.36) Remaining time to fill dewars is 25080 Dewars are NOT busy filling Trimming A by 20 140 20 185, reference by 255 24 20 240 Trimming A by 20 140 20 185, reference by 265 20 175 20 Trimming A by 20 140 20 185, reference by 20 145 20 185 Trimming A by 20 140 20 185, reference by 20 130 145 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.13 Trimming A by 20 20 20 20, reference by 20 145 20 185 Disparity in stage position after aligning to target position: -0.02 0.09 (2nd move error 0.02 0.19) Finished aligning to item in 2 rounds 0.5 0.7 unbinned pixels; 1.9 2.9 nm along two shift axes; -2.8 1.8 nm on specimen axes Total_Shift = 1.731828 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.77 microns changed by -2.27 to target drift = 0.16 nm/sec Measured defocus = -1.44 microns changed by -0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5186.tif 27 frames are being saved to U:\krios\20190717\PilA_5187.tif 27 frames are being saved to U:\krios\20190717\PilA_5188.tif 27 frames are being saved to U:\krios\20190717\PilA_5189.tif 27 frames are being saved to U:\krios\20190717\PilA_5190.tif 27 frames are being saved to U:\krios\20190717\PilA_5191.tif Running script 17 at item 973 (-595.66, -48.24) Remaining time to fill dewars is 25020 Dewars are NOT busy filling Trimming A by 260 24 20 240, reference by 255 24 20 240 Trimming A by 260 24 20 240, reference by 265 20 175 20 Trimming A by 260 24 20 240, reference by 20 145 20 185 Trimming A by 260 24 20 240, reference by 20 130 145 20 Disparity in stage position after aligning to center of map frame: -0.02 -0.14 Trimming A by 99 20 20 180, reference by 255 24 20 240 Disparity in stage position after aligning to target position: 0.18 -0.18 (2nd move error 0.19 -0.07) Finished aligning to item in 2 rounds Trimming A by 102 23 20 170, reference by 99 20 20 180 -6.1 2.0 unbinned pixels; 15.4 18.7 nm along two shift axes; -6.7 -24.0 nm on specimen axes Total_Shift = 12.778233 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.27 microns changed by -3.87 to target drift = 0.07 nm/sec Measured defocus = -1.64 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5192.tif 27 frames are being saved to U:\krios\20190717\PilA_5193.tif 27 frames are being saved to U:\krios\20190717\PilA_5194.tif 27 frames are being saved to U:\krios\20190717\PilA_5195.tif 27 frames are being saved to U:\krios\20190717\PilA_5196.tif 27 frames are being saved to U:\krios\20190717\PilA_5197.tif Running script 17 at item 974 (-594.52, -52.56) Remaining time to fill dewars is 24780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 31 65 20 115, reference by 20 145 20 185 Disparity in stage position after aligning to target position: -0.01 -0.08 (2nd move error 0.04 0.02) Finished aligning to item in 1 rounds Trimming A by 31 65 20 115, reference by 31 65 20 115 -1.1 -2.4 unbinned pixels; 7.4 7.8 nm along two shift axes; 9.7 -3.9 nm on specimen axes Total_Shift = 5.371867 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.79 microns changed by -2.49 to target drift = 0.13 nm/sec Measured defocus = -1.73 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5198.tif 27 frames are being saved to U:\krios\20190717\PilA_5199.tif 27 frames are being saved to U:\krios\20190717\PilA_5200.tif 27 frames are being saved to U:\krios\20190717\PilA_5201.tif 27 frames are being saved to U:\krios\20190717\PilA_5202.tif 27 frames are being saved to U:\krios\20190717\PilA_5203.tif Running script 17 at item 975 (-592.01, -54.28) Remaining time to fill dewars is 24720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 20 90, reference by 20 145 20 185 Disparity in stage position after aligning to target position: 0.00 0.01 (2nd move error 0.06 0.11) Finished aligning to item in 1 rounds Trimming A by 20 75 20 90, reference by 20 80 20 90 0.1 -0.1 unbinned pixels; 0.4 0.3 nm along two shift axes; 0.2 0.4 nm on specimen axes Total_Shift = 0.250418 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.84 microns changed by -3.64 to target drift = 0.16 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5204.tif 27 frames are being saved to U:\krios\20190717\PilA_5205.tif 27 frames are being saved to U:\krios\20190717\PilA_5206.tif 27 frames are being saved to U:\krios\20190717\PilA_5207.tif 27 frames are being saved to U:\krios\20190717\PilA_5208.tif 27 frames are being saved to U:\krios\20190717\PilA_5209.tif Running script 17 at item 976 (-589.48, -55.51) Remaining time to fill dewars is 24480 Dewars are NOT busy filling Trimming A by 24 135 140 20, reference by 255 24 20 240 Trimming A by 24 135 140 20, reference by 265 20 175 20 Trimming A by 24 135 140 20, reference by 20 145 20 185 Trimming A by 24 135 140 20, reference by 20 130 145 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.11 Trimming A by 24 85 20 70, reference by 20 130 145 20 Disparity in stage position after aligning to target position: -0.10 -0.37 (2nd move error -0.06 -0.29) Finished aligning to item in 2 rounds Trimming A by 21 90 55 70, reference by 24 85 20 70 -30.4 12.1 unbinned pixels; 84.1 91.3 nm along two shift axes; -40.6 -120.8 nm on specimen axes Total_Shift = 65.441844 Trimming A by 24 90 20 75, reference by 24 85 20 70 0.2 5.5 unbinned pixels; 19.9 9.0 nm along two shift axes; -21.4 -0.4 nm on specimen axes Total_Shift = 10.994624 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.34 microns changed by -2.24 to target drift = 0.05 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5210.tif 27 frames are being saved to U:\krios\20190717\PilA_5211.tif 27 frames are being saved to U:\krios\20190717\PilA_5212.tif 27 frames are being saved to U:\krios\20190717\PilA_5213.tif 27 frames are being saved to U:\krios\20190717\PilA_5214.tif 27 frames are being saved to U:\krios\20190717\PilA_5215.tif Running script 17 at item 977 (-586.97, -57.19) Remaining time to fill dewars is 24300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 120 75 30, reference by 20 130 145 20 Disparity in stage position after aligning to target position: 0.08 -0.30 (2nd move error 0.13 -0.21) Finished aligning to item in 1 rounds Trimming A by 20 125 75 20, reference by 20 120 75 30 -23.1 -0.5 unbinned pixels; 28.3 84.0 nm along two shift axes; 6.8 -89.8 nm on specimen axes Total_Shift = 46.254777 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.54 microns changed by -3.54 to target drift = 0.12 nm/sec Measured defocus = -2.10 microns changed by 0.10 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5216.tif 27 frames are being saved to U:\krios\20190717\PilA_5217.tif 27 frames are being saved to U:\krios\20190717\PilA_5218.tif 27 frames are being saved to U:\krios\20190717\PilA_5219.tif 27 frames are being saved to U:\krios\20190717\PilA_5220.tif 27 frames are being saved to U:\krios\20190717\PilA_5221.tif Running script 17 at item 978 (-591.14, -58.53) Remaining time to fill dewars is 24180 Dewars are NOT busy filling Trimming A by 20 140 20 185, reference by 255 24 20 240 Trimming A by 20 140 20 185, reference by 265 20 175 20 Trimming A by 20 140 20 185, reference by 20 145 20 185 Trimming A by 20 140 20 185, reference by 20 130 145 20 Disparity in stage position after aligning to center of map frame: -0.03 -0.16 Trimming A by 22 175 20 135, reference by 20 145 20 185 Disparity in stage position after aligning to target position: 0.09 0.06 (2nd move error 0.11 0.20) Finished aligning to item in 2 rounds Trimming A by 22 170 20 130, reference by 22 175 20 135 0.3 0.3 unbinned pixels; 0.8 1.4 nm along two shift axes; -1.3 0.9 nm on specimen axes Total_Shift = 0.803177 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.14 microns changed by -2.24 to target drift = 0.16 nm/sec Measured defocus = -2.10 microns changed by -0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5222.tif 27 frames are being saved to U:\krios\20190717\PilA_5223.tif 27 frames are being saved to U:\krios\20190717\PilA_5224.tif 27 frames are being saved to U:\krios\20190717\PilA_5225.tif 27 frames are being saved to U:\krios\20190717\PilA_5226.tif 27 frames are being saved to U:\krios\20190717\PilA_5227.tif Running script 17 at item 979 (-593.55, -56.80) Remaining time to fill dewars is 23940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 150 20 150, reference by 20 145 20 185 Disparity in stage position after aligning to target position: -0.01 -0.08 (2nd move error 0.01 0.06) Finished aligning to item in 1 rounds Trimming A by 21 150 60 145, reference by 22 150 20 150 -2.1 -0.7 unbinned pixels; 0.0 8.5 nm along two shift axes; 3.2 -7.8 nm on specimen axes Total_Shift = 4.349153 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.37 microns changed by -3.57 to target drift = 0.13 nm/sec Measured defocus = -2.16 microns changed by -0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5228.tif 27 frames are being saved to U:\krios\20190717\PilA_5229.tif 27 frames are being saved to U:\krios\20190717\PilA_5230.tif 27 frames are being saved to U:\krios\20190717\PilA_5231.tif 27 frames are being saved to U:\krios\20190717\PilA_5232.tif 27 frames are being saved to U:\krios\20190717\PilA_5233.tif Running script 17 at item 980 (-636.99, -18.35) Remaining time to fill dewars is 23820 Dewars are NOT busy filling Trimming A by 250 24 165 20, reference by 260 23 20 285 Trimming A by 250 24 165 20, reference by 245 22 160 20 Trimming A by 250 24 165 20, reference by 22 195 50 245 Trimming A by 250 24 165 20, reference by 24 155 125 20 Disparity in stage position after aligning to center of map frame: -0.16 -0.10 Trimming A by 110 21 150 20, reference by 245 22 160 20 Disparity in stage position after aligning to target position: -0.09 -0.10 (2nd move error 0.06 -0.02) Finished aligning to item in 2 rounds Trimming A by 110 21 150 20, reference by 110 21 150 20 -4.4 0.9 unbinned pixels; 9.1 14.5 nm along two shift axes; -2.6 -17.4 nm on specimen axes Total_Shift = 9.009734 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.12 microns changed by -2.18 to target drift = 0.11 nm/sec Measured defocus = -2.34 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5234.tif 27 frames are being saved to U:\krios\20190717\PilA_5235.tif 27 frames are being saved to U:\krios\20190717\PilA_5236.tif 27 frames are being saved to U:\krios\20190717\PilA_5237.tif 27 frames are being saved to U:\krios\20190717\PilA_5238.tif 27 frames are being saved to U:\krios\20190717\PilA_5239.tif Running script 17 at item 981 (-638.43, -20.90) Remaining time to fill dewars is 23640 Dewars are NOT busy filling Trimming A by 24 155 125 20, reference by 260 23 20 285 Trimming A by 24 155 125 20, reference by 245 22 160 20 Trimming A by 24 155 125 20, reference by 22 195 50 245 Trimming A by 24 155 125 20, reference by 24 155 125 20 Disparity in stage position after aligning to center of map frame: -0.12 -0.16 Trimming A by 23 36 130 20, reference by 24 155 125 20 Disparity in stage position after aligning to target position: -0.16 -0.08 (2nd move error -0.05 0.05) Finished aligning to item in 2 rounds Trimming A by 21 47 120 20, reference by 23 36 130 20 0.5 10.6 unbinned pixels; 38.4 17.7 nm along two shift axes; -41.4 -0.3 nm on specimen axes Total_Shift = 21.254638 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 1.06 microns changed by -3.46 to target drift = 0.16 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5240.tif 27 frames are being saved to U:\krios\20190717\PilA_5241.tif 27 frames are being saved to U:\krios\20190717\PilA_5242.tif 27 frames are being saved to U:\krios\20190717\PilA_5243.tif 27 frames are being saved to U:\krios\20190717\PilA_5244.tif 27 frames are being saved to U:\krios\20190717\PilA_5245.tif Running script 17 at item 982 (-640.10, -23.51) Remaining time to fill dewars is 23400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 95 85 20, reference by 24 155 125 20 Disparity in stage position after aligning to target position: -0.13 -0.20 (2nd move error -0.02 -0.07) Finished aligning to item in 1 rounds Trimming A by 20 90 90 20, reference by 23 95 85 20 -7.0 18.4 unbinned pixels; 76.9 2.5 nm along two shift axes; -70.2 -31.1 nm on specimen axes Total_Shift = 39.428736 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.30 microns changed by -2.20 to target drift = 0.05 nm/sec Measured defocus = -2.55 microns changed by 0.05 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5246.tif 27 frames are being saved to U:\krios\20190717\PilA_5247.tif 27 frames are being saved to U:\krios\20190717\PilA_5248.tif 27 frames are being saved to U:\krios\20190717\PilA_5249.tif 27 frames are being saved to U:\krios\20190717\PilA_5250.tif 27 frames are being saved to U:\krios\20190717\PilA_5251.tif Running script 17 at item 983 (-641.70, -26.17) Remaining time to fill dewars is 23280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 130 70 55, reference by 24 155 125 20 Disparity in stage position after aligning to target position: -0.08 -0.23 (2nd move error 0.04 -0.10) Finished aligning to item in 1 rounds Trimming A by 20 130 70 60, reference by 20 130 70 55 -13.0 0.3 unbinned pixels; 18.1 46.3 nm along two shift axes; 1.5 -50.6 nm on specimen axes Total_Shift = 25.975683 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.96 microns changed by -3.56 to target drift = 0.08 nm/sec Measured defocus = -2.68 microns changed by 0.08 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5252.tif 27 frames are being saved to U:\krios\20190717\PilA_5253.tif 27 frames are being saved to U:\krios\20190717\PilA_5254.tif 27 frames are being saved to U:\krios\20190717\PilA_5255.tif 27 frames are being saved to U:\krios\20190717\PilA_5256.tif 27 frames are being saved to U:\krios\20190717\PilA_5257.tif Running script 17 at item 984 (-639.54, -16.65) Remaining time to fill dewars is 23160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 125 21 115 20, reference by 245 22 160 20 Disparity in stage position after aligning to target position: -0.02 -0.19 (2nd move error 0.14 -0.12) Finished aligning to item in 1 rounds Trimming A by 125 21 115 20, reference by 125 21 115 20 -13.1 -2.2 unbinned pixels; 8.8 50.4 nm along two shift axes; 11.5 -50.4 nm on specimen axes Total_Shift = 26.527032 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.36 microns changed by -2.26 to target drift = 0.09 nm/sec Measured defocus = -0.91 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5258.tif 27 frames are being saved to U:\krios\20190717\PilA_5259.tif 27 frames are being saved to U:\krios\20190717\PilA_5260.tif 27 frames are being saved to U:\krios\20190717\PilA_5261.tif 27 frames are being saved to U:\krios\20190717\PilA_5262.tif 27 frames are being saved to U:\krios\20190717\PilA_5263.tif Running script 17 at item 985 (-640.94, -19.22) Remaining time to fill dewars is 22860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 20 55 20, reference by 24 155 125 20 Disparity in stage position after aligning to target position: -0.22 -0.27 (2nd move error -0.11 -0.14) Finished aligning to item in 1 rounds Trimming A by 55 20 55 20, reference by 55 20 55 20 -13.7 21.5 unbinned pixels; 97.0 16.8 nm along two shift axes; -80.9 -57.7 nm on specimen axes Total_Shift = 50.994232 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.68 microns changed by -3.68 to target drift = 0.05 nm/sec Measured defocus = -1.05 microns changed by 0.05 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5264.tif 27 frames are being saved to U:\krios\20190717\PilA_5265.tif 27 frames are being saved to U:\krios\20190717\PilA_5266.tif 27 frames are being saved to U:\krios\20190717\PilA_5267.tif 27 frames are being saved to U:\krios\20190717\PilA_5268.tif 27 frames are being saved to U:\krios\20190717\PilA_5269.tif Running script 17 at item 986 (-642.59, -21.84) Remaining time to fill dewars is 22680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 30 20 30, reference by 24 155 125 20 Disparity in stage position after aligning to target position: -0.17 -0.24 (2nd move error -0.05 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 45 20 20, reference by 20 30 20 30 -14.6 11.5 unbinned pixels; 61.2 35.2 nm along two shift axes; -41.5 -59.1 nm on specimen axes Total_Shift = 37.078568 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.57 microns changed by -3.67 to target drift = 0.09 nm/sec Measured defocus = -1.15 microns changed by 0.05 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5270.tif 27 frames are being saved to U:\krios\20190717\PilA_5271.tif 27 frames are being saved to U:\krios\20190717\PilA_5272.tif 27 frames are being saved to U:\krios\20190717\PilA_5273.tif 27 frames are being saved to U:\krios\20190717\PilA_5274.tif 27 frames are being saved to U:\krios\20190717\PilA_5275.tif Running script 17 at item 987 (-644.18, -24.45) Remaining time to fill dewars is 22440 Dewars are NOT busy filling Trimming A by 24 155 125 20, reference by 260 23 20 285 Trimming A by 24 155 125 20, reference by 245 22 160 20 Trimming A by 24 155 125 20, reference by 22 195 50 245 Trimming A by 24 155 125 20, reference by 24 155 125 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.13 Trimming A by 20 86 20 105, reference by 24 155 125 20 Disparity in stage position after aligning to target position: -0.10 -0.31 (2nd move error -0.05 -0.21) Finished aligning to item in 2 rounds Trimming A by 21 91 20 105, reference by 20 86 20 105 -25.3 10.9 unbinned pixels; 73.0 74.8 nm along two shift axes; -37.0 -100.8 nm on specimen axes Total_Shift = 55.109295 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.47 microns changed by -3.67 to target drift = 0.03 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5276.tif 27 frames are being saved to U:\krios\20190717\PilA_5277.tif 27 frames are being saved to U:\krios\20190717\PilA_5278.tif 27 frames are being saved to U:\krios\20190717\PilA_5279.tif 27 frames are being saved to U:\krios\20190717\PilA_5280.tif 27 frames are being saved to U:\krios\20190717\PilA_5281.tif Running script 17 at item 988 (-641.97, -14.95) Remaining time to fill dewars is 22260 Dewars are NOT busy filling Trimming A by 251 21 160 20, reference by 260 23 20 285 Trimming A by 251 21 160 20, reference by 245 22 160 20 Trimming A by 251 21 160 20, reference by 22 195 50 245 Trimming A by 251 21 160 20, reference by 24 155 125 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.10 Trimming A by 150 21 95 20, reference by 245 22 160 20 Disparity in stage position after aligning to target position: 0.00 -0.27 (2nd move error 0.04 -0.19) Finished aligning to item in 2 rounds Trimming A by 145 21 95 20, reference by 150 21 95 20 -18.3 -1.9 unbinned pixels; 16.8 68.7 nm along two shift axes; 11.3 -70.7 nm on specimen axes Total_Shift = 36.753366 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.35 microns changed by -3.65 to target drift = 0.08 nm/sec Measured defocus = -1.39 microns changed by 0.09 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5282.tif 27 frames are being saved to U:\krios\20190717\PilA_5283.tif 27 frames are being saved to U:\krios\20190717\PilA_5284.tif 27 frames are being saved to U:\krios\20190717\PilA_5285.tif 27 frames are being saved to U:\krios\20190717\PilA_5286.tif 27 frames are being saved to U:\krios\20190717\PilA_5287.tif Running script 17 at item 989 (-643.64, -17.54) Remaining time to fill dewars is 22080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 21 40 55, reference by 245 22 160 20 Disparity in stage position after aligning to target position: -0.07 -0.25 (2nd move error -0.02 -0.17) Finished aligning to item in 1 rounds Trimming A by 65 21 35 55, reference by 65 21 40 55 -22.5 6.5 unbinned pixels; 53.2 71.1 nm along two shift axes; -20.5 -88.7 nm on specimen axes Total_Shift = 46.752621 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 2.26 microns changed by -3.66 to target drift = 0.04 nm/sec Measured defocus = -1.45 microns changed by 0.05 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5288.tif 27 frames are being saved to U:\krios\20190717\PilA_5289.tif 27 frames are being saved to U:\krios\20190717\PilA_5290.tif 27 frames are being saved to U:\krios\20190717\PilA_5291.tif 27 frames are being saved to U:\krios\20190717\PilA_5292.tif 27 frames are being saved to U:\krios\20190717\PilA_5293.tif Running script 17 at item 990 (-645.05, -20.04) Remaining time to fill dewars is 21900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 21 20 75, reference by 24 155 125 20 Disparity in stage position after aligning to target position: -0.18 -0.32 (2nd move error -0.14 -0.22) Finished aligning to item in 1 rounds Trimming A by 25 22 20 70, reference by 21 21 20 75 -22.4 27.4 unbinned pixels; 130.1 39.5 nm along two shift axes; -101.9 -93.1 nm on specimen axes Total_Shift = 70.865249 Trimming A by 35 20 20 60, reference by 21 21 20 75 0.7 17.4 unbinned pixels; 63.1 28.7 nm along two shift axes; -67.9 -1.0 nm on specimen axes Total_Shift = 34.837734 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.90 microns changed by -2.40 to target drift = 0.04 nm/sec Measured defocus = -1.55 microns changed by 0.05 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5294.tif 27 frames are being saved to U:\krios\20190717\PilA_5295.tif 27 frames are being saved to U:\krios\20190717\PilA_5296.tif 27 frames are being saved to U:\krios\20190717\PilA_5297.tif 27 frames are being saved to U:\krios\20190717\PilA_5298.tif 27 frames are being saved to U:\krios\20190717\PilA_5299.tif Running script 17 at item 991 (-646.74, -23.10) Remaining time to fill dewars is 21660 Dewars are NOT busy filling Trimming A by 22 190 50 245, reference by 260 23 20 285 Trimming A by 22 190 50 245, reference by 245 22 160 20 Trimming A by 22 190 50 245, reference by 22 195 50 245 Trimming A by 22 190 50 245, reference by 24 155 125 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.11 Trimming A by 20 100 20 120, reference by 22 195 50 245 Disparity in stage position after aligning to target position: -0.01 0.05 (2nd move error 0.04 0.14) Finished aligning to item in 2 rounds Trimming A by 21 95 20 120, reference by 20 100 20 120 1.6 1.4 unbinned pixels; 2.9 7.8 nm along two shift axes; -5.7 5.9 nm on specimen axes Total_Shift = 4.192016 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 2.10 microns changed by -3.70 to target drift = 0.15 nm/sec Measured defocus = -1.65 microns changed by 0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5300.tif 27 frames are being saved to U:\krios\20190717\PilA_5301.tif 27 frames are being saved to U:\krios\20190717\PilA_5302.tif 27 frames are being saved to U:\krios\20190717\PilA_5303.tif 27 frames are being saved to U:\krios\20190717\PilA_5304.tif 27 frames are being saved to U:\krios\20190717\PilA_5305.tif Running script 17 at item 992 (-647.78, -18.84) Remaining time to fill dewars is 21480 Dewars are NOT busy filling Trimming A by 270 24 20 280, reference by 260 23 20 285 Trimming A by 270 24 20 280, reference by 245 22 160 20 Trimming A by 270 24 20 280, reference by 22 195 50 245 Trimming A by 270 24 20 280, reference by 24 155 125 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.12 Trimming A by 77 21 20 150, reference by 260 23 20 285 Disparity in stage position after aligning to target position: 0.19 0.02 (2nd move error 0.23 0.11) Finished aligning to item in 2 rounds Trimming A by 65 22 20 150, reference by 77 21 20 150 1.0 1.0 unbinned pixels; 2.5 5.3 nm along two shift axes; -4.3 3.8 nm on specimen axes Total_Shift = 2.934511 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.63 microns changed by -2.33 to target drift = 0.15 nm/sec Measured defocus = -1.65 microns changed by -0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5306.tif 27 frames are being saved to U:\krios\20190717\PilA_5307.tif 27 frames are being saved to U:\krios\20190717\PilA_5308.tif 27 frames are being saved to U:\krios\20190717\PilA_5309.tif 27 frames are being saved to U:\krios\20190717\PilA_5310.tif 27 frames are being saved to U:\krios\20190717\PilA_5311.tif Running script 17 at item 993 (-664.89, -76.63) Remaining time to fill dewars is 21360 Dewars are NOT busy filling Trimming A by 24 125 110 20, reference by 210 20 20 235 Trimming A by 24 125 110 20, reference by 180 21 135 20 Trimming A by 24 125 110 20, reference by 21 170 20 200 Trimming A by 24 125 110 20, reference by 21 130 105 20 Disparity in stage position after aligning to center of map frame: -0.12 -0.16 Trimming A by 21 76 150 20, reference by 21 130 105 20 Disparity in stage position after aligning to target position: -0.11 -0.01 (2nd move error -0.01 0.13) Finished aligning to item in 2 rounds Trimming A by 21 36 175 20, reference by 21 76 150 20 -4.4 15.8 unbinned pixels; 63.9 7.8 nm along two shift axes; -60.5 -20.6 nm on specimen axes Total_Shift = 32.803463 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.45 microns changed by -3.25 to target drift = 0.11 nm/sec Measured defocus = -1.82 microns changed by 0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5312.tif 27 frames are being saved to U:\krios\20190717\PilA_5313.tif 27 frames are being saved to U:\krios\20190717\PilA_5314.tif 27 frames are being saved to U:\krios\20190717\PilA_5315.tif 27 frames are being saved to U:\krios\20190717\PilA_5316.tif 27 frames are being saved to U:\krios\20190717\PilA_5317.tif Running script 17 at item 994 (-665.98, -72.34) Remaining time to fill dewars is 21060 Dewars are NOT busy filling Trimming A by 185 21 140 20, reference by 210 20 20 235 Trimming A by 185 21 140 20, reference by 180 21 135 20 Trimming A by 185 21 140 20, reference by 21 170 20 200 Trimming A by 185 21 140 20, reference by 21 130 105 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.15 Trimming A by 125 24 140 20, reference by 180 21 135 20 Disparity in stage position after aligning to target position: 0.02 -0.05 (2nd move error 0.12 0.08) Finished aligning to item in 2 rounds Trimming A by 125 24 145 20, reference by 125 24 140 20 1.0 0.7 unbinned pixels; 1.1 4.6 nm along two shift axes; -2.8 3.8 nm on specimen axes Total_Shift = 2.410634 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = -0.01 microns changed by -1.89 to target drift = 0.13 nm/sec Measured defocus = -1.94 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5318.tif 27 frames are being saved to U:\krios\20190717\PilA_5319.tif 27 frames are being saved to U:\krios\20190717\PilA_5320.tif 27 frames are being saved to U:\krios\20190717\PilA_5321.tif 27 frames are being saved to U:\krios\20190717\PilA_5322.tif 27 frames are being saved to U:\krios\20190717\PilA_5323.tif Running script 17 at item 995 (-668.47, -70.72) Remaining time to fill dewars is 20880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 22 125 20, reference by 180 21 135 20 Disparity in stage position after aligning to target position: -0.06 -0.20 (2nd move error 0.04 -0.07) Finished aligning to item in 1 rounds Trimming A by 145 20 125 20, reference by 140 22 125 20 -8.3 -2.2 unbinned pixels; 2.8 33.4 nm along two shift axes; 10.4 -32.0 nm on specimen axes Total_Shift = 17.27098 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 1.27 microns changed by -3.27 to target drift = 0.11 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5324.tif 27 frames are being saved to U:\krios\20190717\PilA_5325.tif 27 frames are being saved to U:\krios\20190717\PilA_5326.tif 27 frames are being saved to U:\krios\20190717\PilA_5327.tif 27 frames are being saved to U:\krios\20190717\PilA_5328.tif 27 frames are being saved to U:\krios\20190717\PilA_5329.tif Running script 17 at item 996 (-670.96, -69.02) Remaining time to fill dewars is 20700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 155 24 110 20, reference by 180 21 135 20 Disparity in stage position after aligning to target position: -0.11 -0.19 (2nd move error -0.01 -0.05) Finished aligning to item in 1 rounds Trimming A by 155 24 110 20, reference by 155 24 110 20 -8.6 3.6 unbinned pixels; 24.4 25.7 nm along two shift axes; -12.0 -34.3 nm on specimen axes Total_Shift = 18.671136 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 2.49 microns changed by -4.59 to target drift = 1.16 nm/sec Measured defocus = -4.75 microns changed by 2.65 to target drift = 0.03 nm/sec Measured defocus = -3.25 microns changed by 1.15 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5330.tif 27 frames are being saved to U:\krios\20190717\PilA_5331.tif 27 frames are being saved to U:\krios\20190717\PilA_5332.tif 27 frames are being saved to U:\krios\20190717\PilA_5333.tif 27 frames are being saved to U:\krios\20190717\PilA_5334.tif 27 frames are being saved to U:\krios\20190717\PilA_5335.tif Running script 17 at item 997 (-672.55, -71.61) Remaining time to fill dewars is 20400 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 75 24 55 20, reference by 180 21 135 20 Disparity in stage position after aligning to target position: -0.02 -0.23 (2nd move error 0.08 -0.10) Finished aligning to item in 1 rounds Trimming A by 75 24 55 20, reference by 75 24 55 20 -12.4 -2.5 unbinned pixels; 6.9 48.3 nm along two shift axes; 12.4 -47.5 nm on specimen axes Total_Shift = 25.212179 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 1.34 microns changed by -3.54 to target drift = 0.10 nm/sec Measured defocus = -2.27 microns changed by 0.07 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5336.tif 27 frames are being saved to U:\krios\20190717\PilA_5337.tif 27 frames are being saved to U:\krios\20190717\PilA_5338.tif 27 frames are being saved to U:\krios\20190717\PilA_5339.tif 27 frames are being saved to U:\krios\20190717\PilA_5340.tif 27 frames are being saved to U:\krios\20190717\PilA_5341.tif Running script 17 at item 998 (-670.11, -73.29) Remaining time to fill dewars is 20220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 24 55 20, reference by 180 21 135 20 Disparity in stage position after aligning to target position: 0.05 -0.19 (2nd move error 0.15 -0.07) Finished aligning to item in 1 rounds Trimming A by 65 24 60 20, reference by 65 24 55 20 -5.5 -2.3 unbinned pixels; 1.5 23.2 nm along two shift axes; 10.3 -20.8 nm on specimen axes Total_Shift = 11.912733 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.31 microns changed by -1.99 to target drift = 0.14 nm/sec Measured defocus = -2.28 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5342.tif 27 frames are being saved to U:\krios\20190717\PilA_5343.tif 27 frames are being saved to U:\krios\20190717\PilA_5344.tif 27 frames are being saved to U:\krios\20190717\PilA_5345.tif 27 frames are being saved to U:\krios\20190717\PilA_5346.tif 27 frames are being saved to U:\krios\20190717\PilA_5347.tif Running script 17 at item 999 (-669.08, -77.45) Remaining time to fill dewars is 20040 Dewars are NOT busy filling Trimming A by 24 116 115 20, reference by 210 20 20 235 Trimming A by 24 116 115 20, reference by 180 21 135 20 Trimming A by 24 116 115 20, reference by 21 170 20 200 Trimming A by 24 116 115 20, reference by 21 130 105 20 Disparity in stage position after aligning to center of map frame: -0.02 -0.19 Trimming A by 24 35 60 20, reference by 21 130 105 20 Disparity in stage position after aligning to target position: -0.06 -0.21 (2nd move error -0.04 -0.04) Finished aligning to item in 2 rounds Trimming A by 24 35 60 20, reference by 24 35 60 20 -5.8 8.7 unbinned pixels; 39.5 7.7 nm along two shift axes; -32.6 -24.3 nm on specimen axes Total_Shift = 20.86277 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.96 microns changed by -3.36 to target drift = 0.07 nm/sec Measured defocus = -2.48 microns changed by 0.08 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5348.tif 27 frames are being saved to U:\krios\20190717\PilA_5349.tif 27 frames are being saved to U:\krios\20190717\PilA_5350.tif 27 frames are being saved to U:\krios\20190717\PilA_5351.tif 27 frames are being saved to U:\krios\20190717\PilA_5352.tif 27 frames are being saved to U:\krios\20190717\PilA_5353.tif Running script 17 at item 1000 (-671.55, -75.78) Remaining time to fill dewars is 19860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 21 130 105 20 Disparity in stage position after aligning to target position: -0.11 -0.29 (2nd move error -0.10 -0.13) Finished aligning to item in 1 rounds -15.0 19.6 unbinned pixels; 91.8 24.3 nm along two shift axes; -73.3 -62.3 nm on specimen axes Total_Shift = 49.381058 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.43 microns changed by -2.07 to target drift = 0.01 nm/sec Measured defocus = -2.51 microns changed by 0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5354.tif 27 frames are being saved to U:\krios\20190717\PilA_5355.tif 27 frames are being saved to U:\krios\20190717\PilA_5356.tif 27 frames are being saved to U:\krios\20190717\PilA_5357.tif 27 frames are being saved to U:\krios\20190717\PilA_5358.tif 27 frames are being saved to U:\krios\20190717\PilA_5359.tif Running script 17 at item 1001 (-674.17, -74.08) Remaining time to fill dewars is 19620 Dewars are NOT busy filling Trimming A by 180 21 140 20, reference by 210 20 20 235 Trimming A by 180 21 140 20, reference by 180 21 135 20 Trimming A by 180 21 140 20, reference by 21 170 20 200 Trimming A by 180 21 140 20, reference by 21 130 105 20 Disparity in stage position after aligning to center of map frame: -0.01 -0.14 Trimming A by 20 20 20 20, reference by 180 21 135 20 Disparity in stage position after aligning to target position: -0.05 -0.38 (2nd move error -0.04 -0.27) Finished aligning to item in 2 rounds -25.8 8.2 unbinned pixels; 63.7 80.6 nm along two shift axes; -26.3 -102.1 nm on specimen axes Total_Shift = 54.1442 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.95 microns changed by -3.55 to target drift = 0.03 nm/sec Measured defocus = -2.69 microns changed by 0.09 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5360.tif 27 frames are being saved to U:\krios\20190717\PilA_5361.tif 27 frames are being saved to U:\krios\20190717\PilA_5362.tif 27 frames are being saved to U:\krios\20190717\PilA_5363.tif 27 frames are being saved to U:\krios\20190717\PilA_5364.tif 27 frames are being saved to U:\krios\20190717\PilA_5365.tif Running script 17 at item 1002 (-676.78, -72.82) Remaining time to fill dewars is 19440 Dewars are NOT busy filling Trimming A by 210 20 20 235, reference by 210 20 20 235 Trimming A by 210 20 20 235, reference by 180 21 135 20 Trimming A by 210 20 20 235, reference by 21 170 20 200 Trimming A by 210 20 20 235, reference by 21 130 105 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.11 Trimming A by 62 22 20 100, reference by 210 20 20 235 Disparity in stage position after aligning to target position: 0.12 0.04 (2nd move error 0.16 0.13) Finished aligning to item in 2 rounds Trimming A by 66 24 20 95, reference by 62 22 20 100 0.1 -0.5 unbinned pixels; 1.9 0.2 nm along two shift axes; 1.8 0.7 nm on specimen axes Total_Shift = 0.999068 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.39 microns changed by -2.29 to target drift = 0.15 nm/sec Measured defocus = -0.90 microns changed by 0.00 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5366.tif 27 frames are being saved to U:\krios\20190717\PilA_5367.tif 27 frames are being saved to U:\krios\20190717\PilA_5368.tif 27 frames are being saved to U:\krios\20190717\PilA_5369.tif 27 frames are being saved to U:\krios\20190717\PilA_5370.tif 27 frames are being saved to U:\krios\20190717\PilA_5371.tif Running script 17 at item 1003 (-679.28, -71.13) Remaining time to fill dewars is 19260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 132 21 20 165, reference by 210 20 20 235 Disparity in stage position after aligning to target position: 0.03 -0.06 (2nd move error 0.09 0.03) Finished aligning to item in 1 rounds Trimming A by 132 21 20 165, reference by 132 21 20 165 -0.9 -1.4 unbinned pixels; 4.1 5.3 nm along two shift axes; 5.7 -3.1 nm on specimen axes Total_Shift = 3.325616 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.67 microns changed by -3.67 to target drift = 0.16 nm/sec Measured defocus = -0.97 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5372.tif 27 frames are being saved to U:\krios\20190717\PilA_5373.tif 27 frames are being saved to U:\krios\20190717\PilA_5374.tif 27 frames are being saved to U:\krios\20190717\PilA_5375.tif 27 frames are being saved to U:\krios\20190717\PilA_5376.tif 27 frames are being saved to U:\krios\20190717\PilA_5377.tif Running script 17 at item 1004 (-678.26, -75.38) Remaining time to fill dewars is 19020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 43 23 20 110, reference by 210 20 20 235 Disparity in stage position after aligning to target position: 0.23 0.01 (2nd move error 0.29 0.11) Finished aligning to item in 1 rounds Trimming A by 42 22 20 105, reference by 43 23 20 110 -0.4 -6.3 unbinned pixels; 22.7 11.1 nm along two shift axes; 24.7 -0.3 nm on specimen axes Total_Shift = 12.696308 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.54 microns changed by -3.64 to target drift = 0.16 nm/sec Measured defocus = -1.10 microns changed by 0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5378.tif 27 frames are being saved to U:\krios\20190717\PilA_5379.tif 27 frames are being saved to U:\krios\20190717\PilA_5380.tif 27 frames are being saved to U:\krios\20190717\PilA_5381.tif 27 frames are being saved to U:\krios\20190717\PilA_5382.tif 27 frames are being saved to U:\krios\20190717\PilA_5383.tif Running script 17 at item 1005 (-675.75, -77.13) Remaining time to fill dewars is 18900 Dewars are NOT busy filling Trimming A by 21 165 20 200, reference by 210 20 20 235 Trimming A by 21 165 20 200, reference by 180 21 135 20 Trimming A by 21 165 20 200, reference by 21 170 20 200 Trimming A by 21 165 20 200, reference by 21 130 105 20 Disparity in stage position after aligning to center of map frame: -0.02 -0.15 Trimming A by 20 20 20 20, reference by 21 170 20 200 Disparity in stage position after aligning to target position: -0.01 0.09 (2nd move error -0.02 0.22) Finished aligning to item in 2 rounds -4.9 11.2 unbinned pixels; 47.7 0.7 nm along two shift axes; -42.7 -21.4 nm on specimen axes Total_Shift = 24.505147 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.37 microns changed by -3.57 to target drift = 0.13 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5384.tif 27 frames are being saved to U:\krios\20190717\PilA_5385.tif 27 frames are being saved to U:\krios\20190717\PilA_5386.tif 27 frames are being saved to U:\krios\20190717\PilA_5387.tif 27 frames are being saved to U:\krios\20190717\PilA_5388.tif 27 frames are being saved to U:\krios\20190717\PilA_5389.tif Running script 17 at item 1006 (-673.17, -78.29) Remaining time to fill dewars is 18600 Dewars are NOT busy filling Trimming A by 20 120 110 20, reference by 210 20 20 235 Trimming A by 20 120 110 20, reference by 180 21 135 20 Trimming A by 20 120 110 20, reference by 21 170 20 200 Trimming A by 20 120 110 20, reference by 21 130 105 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.16 Trimming A by 20 20 20 20, reference by 21 130 105 20 Disparity in stage position after aligning to target position: -0.11 -0.38 (2nd move error -0.07 -0.25) Finished aligning to item in 2 rounds -27.0 14.5 unbinned pixels; 88.7 75.4 nm along two shift axes; -50.8 -108.1 nm on specimen axes Total_Shift = 61.342051 0.9 10.0 unbinned pixels; 35.5 18.3 nm along two shift axes; -39.0 1.4 nm on specimen axes Total_Shift = 20.039691 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.23 microns changed by -3.53 to target drift = 0.05 nm/sec Measured defocus = -1.39 microns changed by 0.09 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5390.tif 27 frames are being saved to U:\krios\20190717\PilA_5391.tif 27 frames are being saved to U:\krios\20190717\PilA_5392.tif 27 frames are being saved to U:\krios\20190717\PilA_5393.tif 27 frames are being saved to U:\krios\20190717\PilA_5394.tif 27 frames are being saved to U:\krios\20190717\PilA_5395.tif Running script 17 at item 1007 (-670.67, -80.07) Remaining time to fill dewars is 18420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 45 50 20, reference by 21 130 105 20 Disparity in stage position after aligning to target position: -0.08 -0.26 (2nd move error -0.04 -0.12) Finished aligning to item in 1 rounds Trimming A by 20 55 45 20, reference by 22 45 50 20 -20.6 13.2 unbinned pixels; 75.5 54.2 nm along two shift axes; -47.2 -82.8 nm on specimen axes Total_Shift = 48.933841 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.97 microns changed by -3.37 to target drift = 0.05 nm/sec Measured defocus = -1.41 microns changed by 0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5396.tif 27 frames are being saved to U:\krios\20190717\PilA_5397.tif 27 frames are being saved to U:\krios\20190717\PilA_5398.tif 27 frames are being saved to U:\krios\20190717\PilA_5399.tif 27 frames are being saved to U:\krios\20190717\PilA_5400.tif 27 frames are being saved to U:\krios\20190717\PilA_5401.tif Running script 17 at item 1008 (-668.18, -81.79) Remaining time to fill dewars is 18300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 107 125 20, reference by 21 130 105 20 Disparity in stage position after aligning to target position: -0.04 -0.14 (2nd move error -0.00 -0.00) Finished aligning to item in 1 rounds Trimming A by 20 111 120 20, reference by 24 107 125 20 -0.2 0.1 unbinned pixels; 0.6 0.7 nm along two shift axes; -0.3 -0.9 nm on specimen axes Total_Shift = 0.499875 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.75 microns changed by -3.25 to target drift = 0.15 nm/sec Measured defocus = -1.54 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5402.tif 27 frames are being saved to U:\krios\20190717\PilA_5403.tif 27 frames are being saved to U:\krios\20190717\PilA_5404.tif 27 frames are being saved to U:\krios\20190717\PilA_5405.tif 27 frames are being saved to U:\krios\20190717\PilA_5406.tif 27 frames are being saved to U:\krios\20190717\PilA_5407.tif Running script 17 at item 1009 (-669.74, -84.40) Remaining time to fill dewars is 18000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 150 95 20, reference by 21 130 105 20 Disparity in stage position after aligning to target position: 0.05 -0.21 (2nd move error 0.10 -0.08) Finished aligning to item in 1 rounds Trimming A by 23 155 95 20, reference by 20 150 95 20 -6.7 0.0 unbinned pixels; 8.8 24.2 nm along two shift axes; 1.4 -26.1 nm on specimen axes Total_Shift = 13.428018 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.69 microns changed by -3.29 to target drift = 0.07 nm/sec Measured defocus = -1.64 microns changed by 0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5408.tif 27 frames are being saved to U:\krios\20190717\PilA_5409.tif 27 frames are being saved to U:\krios\20190717\PilA_5410.tif 27 frames are being saved to U:\krios\20190717\PilA_5411.tif 27 frames are being saved to U:\krios\20190717\PilA_5412.tif 27 frames are being saved to U:\krios\20190717\PilA_5413.tif Running script 17 at item 1010 (-672.25, -82.64) Remaining time to fill dewars is 17820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 90 50 65, reference by 21 130 105 20 Disparity in stage position after aligning to target position: 0.04 -0.35 (2nd move error 0.09 -0.21) Finished aligning to item in 1 rounds Trimming A by 24 90 20 70, reference by 21 90 50 65 -22.1 -1.1 unbinned pixels; 24.7 81.2 nm along two shift axes; 9.1 -85.7 nm on specimen axes Total_Shift = 44.229226 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.74 microns changed by -3.44 to target drift = 0.09 nm/sec Measured defocus = -1.83 microns changed by 0.13 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5414.tif 27 frames are being saved to U:\krios\20190717\PilA_5415.tif 27 frames are being saved to U:\krios\20190717\PilA_5416.tif 27 frames are being saved to U:\krios\20190717\PilA_5417.tif 27 frames are being saved to U:\krios\20190717\PilA_5418.tif 27 frames are being saved to U:\krios\20190717\PilA_5419.tif Running script 17 at item 1011 (-677.22, -79.74) Remaining time to fill dewars is 17640 Dewars are NOT busy filling Trimming A by 21 165 20 200, reference by 210 20 20 235 Trimming A by 21 165 20 200, reference by 180 21 135 20 Trimming A by 21 165 20 200, reference by 21 170 20 200 Trimming A by 21 165 20 200, reference by 21 130 105 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.14 Trimming A by 20 75 20 80, reference by 21 170 20 200 Disparity in stage position after aligning to target position: -0.04 0.05 (2nd move error -0.00 0.16) Finished aligning to item in 2 rounds Trimming A by 20 70 20 80, reference by 20 75 20 80 -5.5 3.6 unbinned pixels; 20.3 14.3 nm along two shift axes; -12.8 -22.0 nm on specimen axes Total_Shift = 13.06995 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.49 microns changed by -2.29 to target drift = 0.08 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5420.tif 27 frames are being saved to U:\krios\20190717\PilA_5421.tif 27 frames are being saved to U:\krios\20190717\PilA_5422.tif 27 frames are being saved to U:\krios\20190717\PilA_5423.tif 27 frames are being saved to U:\krios\20190717\PilA_5424.tif 27 frames are being saved to U:\krios\20190717\PilA_5425.tif Running script 17 at item 1012 (-680.84, -73.68) Remaining time to fill dewars is 17460 Dewars are NOT busy filling Trimming A by 198 23 20 245, reference by 210 20 20 235 Trimming A by 198 23 20 245, reference by 180 21 135 20 Trimming A by 198 23 20 245, reference by 21 170 20 200 Trimming A by 198 23 20 245, reference by 21 130 105 20 Disparity in stage position after aligning to center of map frame: -0.03 -0.15 Trimming A by 120 23 20 175, reference by 210 20 20 235 Disparity in stage position after aligning to target position: 0.19 -0.12 (2nd move error 0.20 0.01) Finished aligning to item in 2 rounds Trimming A by 110 24 20 175, reference by 120 23 20 175 3.1 -4.3 unbinned pixels; 19.6 4.6 nm along two shift axes; 15.9 12.8 nm on specimen axes Total_Shift = 10.47851 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.76 microns changed by -3.66 to target drift = 0.14 nm/sec Measured defocus = -1.91 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5426.tif 27 frames are being saved to U:\krios\20190717\PilA_5427.tif 27 frames are being saved to U:\krios\20190717\PilA_5428.tif 27 frames are being saved to U:\krios\20190717\PilA_5429.tif 27 frames are being saved to U:\krios\20190717\PilA_5430.tif 27 frames are being saved to U:\krios\20190717\PilA_5431.tif Running script 17 at item 1013 (-678.86, -82.32) Remaining time to fill dewars is 17160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 140 20 150, reference by 21 170 20 200 Disparity in stage position after aligning to target position: -0.03 -0.04 (2nd move error -0.00 0.08) Finished aligning to item in 1 rounds Trimming A by 23 140 20 145, reference by 23 140 20 150 -2.8 14.5 unbinned pixels; 56.7 11.8 nm along two shift axes; -55.6 -13.8 nm on specimen axes Total_Shift = 29.42323 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.31 microns changed by -2.31 to target drift = 0.09 nm/sec Measured defocus = -2.03 microns changed by 0.03 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5432.tif 27 frames are being saved to U:\krios\20190717\PilA_5433.tif 27 frames are being saved to U:\krios\20190717\PilA_5434.tif 27 frames are being saved to U:\krios\20190717\PilA_5435.tif 27 frames are being saved to U:\krios\20190717\PilA_5436.tif 27 frames are being saved to U:\krios\20190717\PilA_5437.tif Running script 17 at item 1014 (-676.35, -83.99) Remaining time to fill dewars is 17040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 155 20 135, reference by 21 170 20 200 Disparity in stage position after aligning to target position: 0.43 0.07 (2nd move error 0.46 0.19) Finished aligning to item in 1 rounds Trimming A by 21 145 20 125, reference by 20 155 20 135 -2.7 -11.3 unbinned pixels; 38.2 26.7 nm along two shift axes; 44.7 -8.0 nm on specimen axes Total_Shift = 23.300385 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.42 microns changed by -3.52 to target drift = 0.15 nm/sec Measured defocus = -2.20 microns changed by 0.10 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5438.tif 27 frames are being saved to U:\krios\20190717\PilA_5439.tif 27 frames are being saved to U:\krios\20190717\PilA_5440.tif 27 frames are being saved to U:\krios\20190717\PilA_5441.tif 27 frames are being saved to U:\krios\20190717\PilA_5442.tif 27 frames are being saved to U:\krios\20190717\PilA_5443.tif Running script 17 at item 1015 (-371.98, -314.54) Remaining time to fill dewars is 16800 Dewars are NOT busy filling Trimming A by 22 76 115 20, reference by 230 21 20 210 Trimming A by 22 76 115 20, reference by 250 23 145 20 Trimming A by 22 76 115 20, reference by 24 115 20 140 Trimming A by 22 76 115 20, reference by 24 80 110 20 Disparity in stage position after aligning to center of map frame: -0.15 -0.19 Trimming A by 21 57 150 20, reference by 24 80 110 20 Disparity in stage position after aligning to target position: -0.19 -0.04 (2nd move error -0.06 0.13) Finished aligning to item in 2 rounds Trimming A by 21 52 150 20, reference by 21 57 150 20 -0.2 14.3 unbinned pixels; 52.7 20.8 nm along two shift axes; -55.5 -3.7 nm on specimen axes Total_Shift = 28.534387 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.04 microns changed by -2.16 to target drift = 0.14 nm/sec Measured defocus = -2.15 microns changed by -0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5444.tif 27 frames are being saved to U:\krios\20190717\PilA_5445.tif 27 frames are being saved to U:\krios\20190717\PilA_5446.tif 27 frames are being saved to U:\krios\20190717\PilA_5447.tif 27 frames are being saved to U:\krios\20190717\PilA_5448.tif 27 frames are being saved to U:\krios\20190717\PilA_5449.tif Running script 17 at item 1016 (-374.51, -312.76) Remaining time to fill dewars is 16560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 75 20 75 20, reference by 24 80 110 20 Disparity in stage position after aligning to target position: -0.18 -0.19 (2nd move error -0.04 -0.02) Finished aligning to item in 1 rounds Trimming A by 75 20 75 20, reference by 75 20 75 20 -4.9 12.0 unbinned pixels; 50.5 0.4 nm along two shift axes; -45.6 -21.6 nm on specimen axes Total_Shift = 25.909719 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.31 microns changed by -3.61 to target drift = 0.07 nm/sec Measured defocus = -2.34 microns changed by 0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5450.tif 27 frames are being saved to U:\krios\20190717\PilA_5451.tif 27 frames are being saved to U:\krios\20190717\PilA_5452.tif 27 frames are being saved to U:\krios\20190717\PilA_5453.tif 27 frames are being saved to U:\krios\20190717\PilA_5454.tif 27 frames are being saved to U:\krios\20190717\PilA_5455.tif Running script 17 at item 1017 (-377.15, -311.12) Remaining time to fill dewars is 16440 Dewars are NOT busy filling Trimming A by 255 20 145 20, reference by 230 21 20 210 Trimming A by 255 20 145 20, reference by 250 23 145 20 Trimming A by 255 20 145 20, reference by 24 115 20 140 Trimming A by 255 20 145 20, reference by 24 80 110 20 Disparity in stage position after aligning to center of map frame: -0.12 -0.14 Trimming A by 85 24 70 20, reference by 250 23 145 20 Disparity in stage position after aligning to target position: -0.01 -0.23 (2nd move error 0.09 -0.12) Finished aligning to item in 2 rounds Trimming A by 85 24 65 20, reference by 85 24 70 20 -12.1 -4.0 unbinned pixels; 1.1 49.5 nm along two shift axes; 18.1 -46.1 nm on specimen axes Total_Shift = 25.442061 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.10 microns changed by -2.29 to target drift = 0.11 nm/sec Measured defocus = -2.39 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5456.tif 27 frames are being saved to U:\krios\20190717\PilA_5457.tif 27 frames are being saved to U:\krios\20190717\PilA_5458.tif 27 frames are being saved to U:\krios\20190717\PilA_5459.tif 27 frames are being saved to U:\krios\20190717\PilA_5460.tif 27 frames are being saved to U:\krios\20190717\PilA_5461.tif Running script 17 at item 1018 (-381.20, -311.96) Remaining time to fill dewars is 16260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 250 23 145 20 Disparity in stage position after aligning to target position: -0.10 -0.32 (2nd move error 0.01 -0.21) Finished aligning to item in 1 rounds -26.4 -0.1 unbinned pixels; 34.0 95.1 nm along two shift axes; 6.0 -102.5 nm on specimen axes Total_Shift = 52.73016 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 1.33 microns changed by -3.83 to target drift = 0.09 nm/sec Measured defocus = -2.55 microns changed by 0.06 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5462.tif 27 frames are being saved to U:\krios\20190717\PilA_5463.tif 27 frames are being saved to U:\krios\20190717\PilA_5464.tif 27 frames are being saved to U:\krios\20190717\PilA_5465.tif 27 frames are being saved to U:\krios\20190717\PilA_5466.tif 27 frames are being saved to U:\krios\20190717\PilA_5467.tif Running script 17 at item 1019 (-378.58, -313.58) Remaining time to fill dewars is 15960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 80 110 20 Disparity in stage position after aligning to target position: -0.14 -0.37 (2nd move error -0.00 -0.19) Finished aligning to item in 1 rounds -24.2 3.2 unbinned pixels; 43.2 82.2 nm along two shift axes; -7.3 -94.7 nm on specimen axes Total_Shift = 48.758322 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.18 microns changed by -2.42 to target drift = 0.11 nm/sec Measured defocus = -2.66 microns changed by 0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5468.tif 27 frames are being saved to U:\krios\20190717\PilA_5469.tif 27 frames are being saved to U:\krios\20190717\PilA_5470.tif 27 frames are being saved to U:\krios\20190717\PilA_5471.tif 27 frames are being saved to U:\krios\20190717\PilA_5472.tif 27 frames are being saved to U:\krios\20190717\PilA_5473.tif Running script 17 at item 1020 (-375.24, -319.68) Remaining time to fill dewars is 15840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 95 100 20, reference by 24 80 110 20 Disparity in stage position after aligning to target position: -0.05 -0.19 (2nd move error 0.08 -0.02) Finished aligning to item in 1 rounds Trimming A by 20 69 125 20, reference by 24 95 100 20 -2.4 1.7 unbinned pixels; 9.2 6.1 nm along two shift axes; -6.0 -9.6 nm on specimen axes Total_Shift = 5.813092 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.59 microns changed by -2.49 to target drift = 0.09 nm/sec Measured defocus = -0.92 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5474.tif 27 frames are being saved to U:\krios\20190717\PilA_5475.tif 27 frames are being saved to U:\krios\20190717\PilA_5476.tif 27 frames are being saved to U:\krios\20190717\PilA_5477.tif 27 frames are being saved to U:\krios\20190717\PilA_5478.tif 27 frames are being saved to U:\krios\20190717\PilA_5479.tif Running script 17 at item 1021 (-376.90, -322.28) Remaining time to fill dewars is 15600 Dewars are NOT busy filling Trimming A by 24 72 115 20, reference by 230 21 20 210 Trimming A by 24 72 115 20, reference by 250 23 145 20 Trimming A by 24 72 115 20, reference by 24 115 20 140 Trimming A by 24 72 115 20, reference by 24 80 110 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.16 Trimming A by 21 103 140 20, reference by 24 80 110 20 Disparity in stage position after aligning to target position: 0.03 -0.24 (2nd move error 0.08 -0.11) Finished aligning to item in 2 rounds Trimming A by 22 140 115 20, reference by 21 103 140 20 -12.4 0.0 unbinned pixels; 16.2 44.7 nm along two shift axes; 2.6 -48.4 nm on specimen axes Total_Shift = 24.863282 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.83 microns changed by -3.83 to target drift = 0.06 nm/sec Measured defocus = -1.06 microns changed by 0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5480.tif 27 frames are being saved to U:\krios\20190717\PilA_5481.tif 27 frames are being saved to U:\krios\20190717\PilA_5482.tif 27 frames are being saved to U:\krios\20190717\PilA_5483.tif 27 frames are being saved to U:\krios\20190717\PilA_5484.tif 27 frames are being saved to U:\krios\20190717\PilA_5485.tif Running script 17 at item 1022 (-379.38, -320.55) Remaining time to fill dewars is 15360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 41 65 75, reference by 24 80 110 20 Disparity in stage position after aligning to target position: -0.03 -0.37 (2nd move error 0.02 -0.22) Finished aligning to item in 1 rounds Trimming A by 22 50 60 75, reference by 21 41 65 75 -24.9 -2.5 unbinned pixels; 23.1 93.4 nm along two shift axes; 15.1 -96.3 nm on specimen axes Total_Shift = 50.015963 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.87 microns changed by -3.97 to target drift = 0.09 nm/sec Measured defocus = -1.25 microns changed by 0.15 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5486.tif 27 frames are being saved to U:\krios\20190717\PilA_5487.tif 27 frames are being saved to U:\krios\20190717\PilA_5488.tif 27 frames are being saved to U:\krios\20190717\PilA_5489.tif 27 frames are being saved to U:\krios\20190717\PilA_5490.tif 27 frames are being saved to U:\krios\20190717\PilA_5491.tif Running script 17 at item 1023 (-381.90, -319.41) Remaining time to fill dewars is 15120 Dewars are NOT busy filling Trimming A by 20 110 20 140, reference by 230 21 20 210 Trimming A by 20 110 20 140, reference by 250 23 145 20 Trimming A by 20 110 20 140, reference by 24 115 20 140 Trimming A by 20 110 20 140, reference by 24 80 110 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.09 Trimming A by 20 60 20 70, reference by 24 115 20 140 Disparity in stage position after aligning to target position: -0.02 0.19 (2nd move error 0.11 0.26) Finished aligning to item in 2 rounds Trimming A by 21 50 20 70, reference by 20 60 20 70 3.3 0.9 unbinned pixels; 1.1 13.3 nm along two shift axes; -4.1 12.8 nm on specimen axes Total_Shift = 6.897127 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.41 microns changed by -2.61 to target drift = 0.15 nm/sec Measured defocus = -1.20 microns changed by -0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5492.tif 27 frames are being saved to U:\krios\20190717\PilA_5493.tif 27 frames are being saved to U:\krios\20190717\PilA_5494.tif 27 frames are being saved to U:\krios\20190717\PilA_5495.tif 27 frames are being saved to U:\krios\20190717\PilA_5496.tif 27 frames are being saved to U:\krios\20190717\PilA_5497.tif Running script 17 at item 1024 (-386.91, -315.91) Remaining time to fill dewars is 15060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 37 22 20 100, reference by 24 115 20 140 Disparity in stage position after aligning to target position: -0.11 -0.02 (2nd move error 0.03 0.05) Finished aligning to item in 1 rounds Trimming A by 37 22 20 100, reference by 37 22 20 100 -3.4 -1.4 unbinned pixels; 0.8 14.2 nm along two shift axes; 6.2 -12.8 nm on specimen axes Total_Shift = 7.293677 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 2.80 microns changed by -4.10 to target drift = 0.07 nm/sec Measured defocus = -1.33 microns changed by 0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5498.tif 27 frames are being saved to U:\krios\20190717\PilA_5499.tif 27 frames are being saved to U:\krios\20190717\PilA_5500.tif 27 frames are being saved to U:\krios\20190717\PilA_5501.tif 27 frames are being saved to U:\krios\20190717\PilA_5502.tif 27 frames are being saved to U:\krios\20190717\PilA_5503.tif Running script 17 at item 1025 (-383.55, -321.96) Remaining time to fill dewars is 14820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 135 20 130, reference by 24 115 20 140 Disparity in stage position after aligning to target position: 0.05 0.15 (2nd move error 0.18 0.21) Finished aligning to item in 1 rounds Trimming A by 24 130 20 125, reference by 24 135 20 130 1.3 -6.3 unbinned pixels; 25.0 4.9 nm along two shift axes; 24.4 6.3 nm on specimen axes Total_Shift = 12.936597 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.31 microns changed by -2.71 to target drift = 0.11 nm/sec Measured defocus = -1.47 microns changed by 0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5504.tif 27 frames are being saved to U:\krios\20190717\PilA_5505.tif 27 frames are being saved to U:\krios\20190717\PilA_5506.tif 27 frames are being saved to U:\krios\20190717\PilA_5507.tif 27 frames are being saved to U:\krios\20190717\PilA_5508.tif 27 frames are being saved to U:\krios\20190717\PilA_5509.tif Running script 17 at item 1026 (-380.96, -323.09) Remaining time to fill dewars is 14580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 118 20 135, reference by 24 80 110 20 Disparity in stage position after aligning to target position: -0.00 -0.35 (2nd move error 0.05 -0.21) Finished aligning to item in 1 rounds Trimming A by 20 118 20 135, reference by 20 118 20 135 -26.9 -3.6 unbinned pixels; 22.1 102.2 nm along two shift axes; 19.5 -104.0 nm on specimen axes Total_Shift = 54.29751 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 2.40 microns changed by -3.90 to target drift = 0.13 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5510.tif 27 frames are being saved to U:\krios\20190717\PilA_5511.tif 27 frames are being saved to U:\krios\20190717\PilA_5512.tif 27 frames are being saved to U:\krios\20190717\PilA_5513.tif 27 frames are being saved to U:\krios\20190717\PilA_5514.tif 27 frames are being saved to U:\krios\20190717\PilA_5515.tif Running script 17 at item 1027 (-378.45, -324.76) Remaining time to fill dewars is 14400 Dewars are NOT busy filling Trimming A by 22 76 115 20, reference by 230 21 20 210 Trimming A by 22 76 115 20, reference by 250 23 145 20 Trimming A by 22 76 115 20, reference by 24 115 20 140 Trimming A by 22 76 115 20, reference by 24 80 110 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.11 Trimming A by 20 160 90 75, reference by 24 80 110 20 Disparity in stage position after aligning to target position: 0.01 -0.26 (2nd move error 0.05 -0.17) Finished aligning to item in 2 rounds Trimming A by 20 179 90 55, reference by 20 160 90 75 -20.7 -1.0 unbinned pixels; 23.2 76.2 nm along two shift axes; 8.4 -80.5 nm on specimen axes Total_Shift = 41.526364 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.90 microns changed by -2.50 to target drift = 0.13 nm/sec Measured defocus = -1.65 microns changed by 0.05 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5516.tif 27 frames are being saved to U:\krios\20190717\PilA_5517.tif 27 frames are being saved to U:\krios\20190717\PilA_5518.tif 27 frames are being saved to U:\krios\20190717\PilA_5519.tif 27 frames are being saved to U:\krios\20190717\PilA_5520.tif 27 frames are being saved to U:\krios\20190717\PilA_5521.tif The original displacements in the overlap zones have mean 26.71 maximum 260.14 pixels. After shifting pieces into register, the displacements have mean 12.63 maximum 55.76 pixels. Running script 6 at item 14 (-892.66, 725.92) Trimming A by 95 100, reference by 110 110 Trimming A by 75 80, reference by 110 110 Rough eucentricity: changing Z by -66.44 to 54.17, continuing... Rough eucentricity: changing Z by -1.05 to 53.12, finished. Running script 6 at item 14 (-892.66, 725.92) Rough eucentricity: changing Z by 0.96 to 54.08, finished. Z = 0: The original displacements in the overlap zones have mean 16.24 maximum 20.89 pixels. After shifting pieces into register, the displacements have mean 1.06 maximum 1.06 pixels. Running script 6 at item 15 (-711.21, 645.55) Trimming A by 80 85, reference by 95 95 Trimming A by 55 60, reference by 95 95 Rough eucentricity: changing Z by -60.72 to 59.90, continuing... Rough eucentricity: changing Z by -1.25 to 58.64, finished. Z = 1: The original displacements in the overlap zones have mean 14.71 maximum 17.85 pixels. After shifting pieces into register, the displacements have mean 2.93 maximum 2.93 pixels. Running script 6 at item 16 (-623.22, 652.09) Trimming A by 90 95, reference by 105 105 Trimming A by 70 70, reference by 105 105 Rough eucentricity: changing Z by -59.89 to 60.73, continuing... Rough eucentricity: changing Z by 0.23 to 60.95, finished. Z = 2: The original displacements in the overlap zones have mean 14.87 maximum 25.39 pixels. After shifting pieces into register, the displacements have mean 9.15 maximum 9.15 pixels. Running script 6 at item 17 (-536.45, 656.21) Trimming A by 55 60, reference by 65 70 Trimming A by 35 35, reference by 65 70 Rough eucentricity: changing Z by -55.53 to 65.08, continuing... Rough eucentricity: changing Z by -1.79 to 63.29, finished. Z = 3: The original displacements in the overlap zones have mean 17.56 maximum 28.91 pixels. After shifting pieces into register, the displacements have mean 11.50 maximum 11.50 pixels. Running script 6 at item 18 (-579.38, 608.48) Trimming A by 105 105, reference by 115 115 Trimming A by 85 85, reference by 115 115 Rough eucentricity: changing Z by -57.80 to 62.82, continuing... Rough eucentricity: changing Z by -1.43 to 61.38, finished. Z = 4: The original displacements in the overlap zones have mean 17.75 maximum 29.54 pixels. After shifting pieces into register, the displacements have mean 9.00 maximum 9.00 pixels. Running script 6 at item 19 (-533.33, 566.93) Trimming A by 140 140, reference by 150 150 Trimming A by 120 120, reference by 150 150 Rough eucentricity: changing Z by -57.11 to 63.50, continuing... Trimming A by 25 50, reference by 30 50 Trimming A by 30 45, reference by 30 50 Trimming A by 30 45, reference by 30 50 Rough eucentricity: changing Z by -1.06 to 62.44, finished. Z = 5: The original displacements in the overlap zones have mean 18.42 maximum 21.64 pixels. After shifting pieces into register, the displacements have mean 5.94 maximum 5.94 pixels. Running script 6 at item 20 (-489.85, 614.98) Trimming A by 100 100, reference by 110 115 Trimming A by 75 80, reference by 110 115 Rough eucentricity: changing Z by -55.33 to 65.29, continuing... Rough eucentricity: changing Z by -1.62 to 63.67, finished. Z = 6: The original displacements in the overlap zones have mean 15.68 maximum 24.42 pixels. After shifting pieces into register, the displacements have mean 8.35 maximum 8.35 pixels. Running script 6 at item 21 (-270.95, 582.80) Trimming A by 35 35, reference by 40 45 Trimming A by 20 20, reference by 40 45 Rough eucentricity: changing Z by -49.68 to 70.94, continuing... Rough eucentricity: changing Z by -2.24 to 68.70, finished. Z = 7: The original displacements in the overlap zones have mean 12.92 maximum 25.35 pixels. After shifting pieces into register, the displacements have mean 3.06 maximum 3.06 pixels. Running script 6 at item 22 (-225.46, 541.42) Trimming A by 75 75, reference by 85 85 Trimming A by 46 70, reference by 85 85 Rough eucentricity: changing Z by -49.95 to 70.66, continuing... Ending Navigator acquisition after current item Rough eucentricity: changing Z by -1.54 to 69.12, finished. Z = 8: The original displacements in the overlap zones have mean 13.20 maximum 16.87 pixels. After shifting pieces into register, the displacements have mean 3.05 maximum 3.05 pixels. Rough eucentricity: changing Z by -4.17 to 64.95, finished. Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.02 microns and lateral displacement of -1.74 microns Drawn line is 133.2 pixels, 2.076 um at -43.5 degrees Measured defocus = 1.68 microns drift = 0.05 nm/sec Changing defocus to -0.90 to get an acceptable number of CTF rings Ctffind: defocus: -1.070, astig: 0.006 um, angle: -27.1, score 0.7911, fit to 3.0 A Objective stigmator was adjusted by 0.0002 0.0000 Measured defocus = -0.19 microns drift = 0.05 nm/sec Changing defocus to -0.90 to get an acceptable number of CTF rings Ctffind: defocus: -0.910, astig: 0.005 um, angle: 46.8, score 0.7966, fit to 2.9 A Objective stigmator was adjusted by -0.0001 -0.0001 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.395, astig: 0.010 um, angle: -51.7, score 0.7425, fit to 3.1 A Ctffind: defocus: -1.003, astig: 0.395 um, angle: 58.3, score 0.7716, fit to 2.7 A Ctffind: defocus: -1.031, astig: 0.369 um, angle: -78.5, score 0.7834, fit to 2.6 A Ctffind: defocus: -1.048, astig: 0.352 um, angle: -31.5, score 0.7675, fit to 2.7 A Ctffind: defocus: -1.049, astig: 0.351 um, angle: 14.0, score 0.7709, fit to 2.7 A Ctffind: defocus: -1.041, astig: 0.351 um, angle: 60.9, score 0.7757, fit to 2.9 A Ctffind: defocus: -1.024, astig: 0.385 um, angle: -73.0, score 0.7734, fit to 2.7 A Ctffind: defocus: -1.005, astig: 0.401 um, angle: -29.6, score 0.7705, fit to 2.6 A Ctffind: defocus: -0.994, astig: 0.400 um, angle: 13.7, score 0.7624, fit to 2.9 A Ctffind: defocus: -1.408, astig: 0.000 um, angle: 9.2, score 0.7622, fit to 3.5 A Beam tilt was adjusted by 0.19 -0.23 milliradians Measuring at image shift -0.9 0.0 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.379, astig: 0.047 um, angle: -72.4, score 0.7081, fit to 2.8 A Ctffind: defocus: -0.988, astig: 0.399 um, angle: 63.7, score 0.7151, fit to 2.8 A Ctffind: defocus: -1.020, astig: 0.357 um, angle: 61.5, score 0.7114, fit to 3.2 A Ctffind: defocus: -0.994, astig: 0.390 um, angle: -34.3, score 0.6879, fit to 2.5 A Ctffind: defocus: -1.021, astig: 0.366 um, angle: -33.0, score 0.7085, fit to 2.8 A Beam tilt needs to be adjusted by 0.16 0.15 milliradians Measuring at image shift 0.9 0.0 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.408, astig: 0.035 um, angle: 8.4, score 0.7694, fit to 2.9 A Ctffind: defocus: -1.047, astig: 0.365 um, angle: 55.6, score 0.7786, fit to 2.7 A Ctffind: defocus: -1.016, astig: 0.388 um, angle: 57.4, score 0.7723, fit to 2.8 A Ctffind: defocus: -1.051, astig: 0.363 um, angle: -25.8, score 0.7710, fit to 2.8 A Ctffind: defocus: -1.019, astig: 0.396 um, angle: -29.3, score 0.7744, fit to 2.6 A Beam tilt needs to be adjusted by -0.17 -0.17 milliradians Measuring at image shift 0.0 -0.9 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.418, astig: 0.006 um, angle: 15.2, score 0.7724, fit to 3.0 A Ctffind: defocus: -1.026, astig: 0.400 um, angle: 57.8, score 0.7842, fit to 2.8 A Ctffind: defocus: -1.053, astig: 0.363 um, angle: 59.5, score 0.7870, fit to 2.7 A Ctffind: defocus: -1.061, astig: 0.351 um, angle: -30.2, score 0.7852, fit to 2.7 A Ctffind: defocus: -1.029, astig: 0.388 um, angle: -29.1, score 0.7614, fit to 2.5 A Beam tilt needs to be adjusted by 0.14 -0.17 milliradians Measuring at image shift 0.0 0.9 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.376, astig: 0.020 um, angle: -56.9, score 0.6953, fit to 3.0 A Ctffind: defocus: -1.014, astig: 0.359 um, angle: 62.0, score 0.6864, fit to 2.6 A Ctffind: defocus: -0.985, astig: 0.379 um, angle: 59.0, score 0.6695, fit to 3.1 A Ctffind: defocus: -0.986, astig: 0.393 um, angle: -29.9, score 0.6614, fit to 2.8 A Ctffind: defocus: -1.015, astig: 0.373 um, angle: -32.9, score 0.6877, fit to 2.7 A Beam tilt needs to be adjusted by -0.16 0.16 milliradians IS to beam tilt matrix: -0.187170 -0.164258 -0.174351 0.182729 Running script 17 at item 47 (-888.40, 728.34) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 190 24 160 20, reference by 215 23 20 210 Trimming A by 190 24 160 20, reference by 185 22 155 20 Trimming A by 190 24 160 20, reference by 23 160 20 185 Trimming A by 190 24 160 20, reference by 24 130 120 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.09 Trimming A by 57 22 80 20, reference by 185 22 155 20 Disparity in stage position after aligning to target position: -0.01 -0.11 (2nd move error -0.08 -0.04) Finished aligning to item in 2 rounds Trimming A by 52 22 85 20, reference by 57 22 80 20 -1.3 41.2 unbinned pixels; 153.2 57.5 nm along two shift axes; -160.1 -13.6 nm on specimen axes Total_Shift = 82.46263 Trimming A by 57 22 80 20, reference by 57 22 80 20 0.2 29.5 unbinned pixels; 108.2 45.2 nm along two shift axes; -114.8 -5.3 nm on specimen axes Total_Shift = 58.997498 Target defocus is: -1.60 um -0.00 degrees Target defocus is: -1.70 um Using peak at -29.3,86.8, rejecting 1.93 times higher peak at -0.1,0.4 Measured defocus = -0.48 microns changed by -1.22 to target drift = 1.03 nm/sec 27 frames were saved to U:\krios\20190717\PilA_5523.tif 27 frames were saved to U:\krios\20190717\PilA_5524.tif 27 frames were saved to U:\krios\20190717\PilA_5525.tif 27 frames were saved to U:\krios\20190717\PilA_5526.tif 27 frames were saved to U:\krios\20190717\PilA_5527.tif 27 frames were saved to U:\krios\20190717\PilA_5528.tif Running script 6 at item 23 (53.10, 285.67) Trimming A by 95 95, reference by 105 105 Trimming A by 66 90, reference by 105 105 Rough eucentricity: changing Z by -52.06 to 68.56, continuing... Rough eucentricity: changing Z by -0.98 to 67.57, finished. Z = 9: The original displacements in the overlap zones have mean 15.16 maximum 22.19 pixels. After shifting pieces into register, the displacements have mean 5.15 maximum 5.15 pixels. Running script 6 at item 24 (58.64, 195.70) Trimming A by 135 135, reference by 150 150 Trimming A by 80 80, reference by 150 150 Rough eucentricity: changing Z by -54.34 to 66.28, continuing... Rough eucentricity: changing Z by -0.82 to 65.46, finished. Z = 10: The original displacements in the overlap zones have mean 22.45 maximum 29.74 pixels. After shifting pieces into register, the displacements have mean 14.18 maximum 14.18 pixels. Running script 6 at item 25 (-85.08, 412.65) Trimming A by 105 105, reference by 115 115 Trimming A by 85 85, reference by 115 115 Rough eucentricity: changing Z by -49.71 to 70.91, continuing... Rough eucentricity: changing Z by -1.35 to 69.56, finished. Z = 11: The original displacements in the overlap zones have mean 13.31 maximum 18.56 pixels. After shifting pieces into register, the displacements have mean 4.04 maximum 4.04 pixels. Running script 6 at item 26 (-126.35, 365.52) Trimming A by 110 115, reference by 120 125 Trimming A by 90 95, reference by 120 125 Rough eucentricity: changing Z by -51.47 to 69.15, continuing... Rough eucentricity: changing Z by -1.32 to 67.83, finished. Z = 12: The original displacements in the overlap zones have mean 13.46 maximum 17.51 pixels. After shifting pieces into register, the displacements have mean 4.20 maximum 4.20 pixels. Running script 6 at item 27 (-666.28, 604.32) Trimming A by 120 125, reference by 130 135 Trimming A by 100 100, reference by 130 135 Rough eucentricity: changing Z by -59.65 to 60.97, continuing... Trimming A by 20 35, reference by 20 35 Trimming A by 20 35, reference by 20 35 Trimming A by 20 35, reference by 20 35 Rough eucentricity: changing Z by -1.69 to 59.28, finished. Z = 13: The original displacements in the overlap zones have mean 10.72 maximum 17.97 pixels. After shifting pieces into register, the displacements have mean 7.30 maximum 7.30 pixels. Running script 6 at item 28 (154.51, 21.21) Trimming A by 125 130, reference by 140 140 Trimming A by 105 105, reference by 140 140 Trimming A by 75 75, reference by 140 140 Rough eucentricity: changing Z by -33.85 to 86.76, continuing... Trimming A by 20 20 20 20, reference by 40 20 20 40 Rough eucentricity: changing Z by -27.51 to 59.25, continuing... Rough eucentricity: changing Z by 0.79 to 60.04, finished. Z = 14: The original displacements in the overlap zones have mean 23.82 maximum 46.95 pixels. After shifting pieces into register, the displacements have mean 11.40 maximum 11.40 pixels. Running script 6 at item 29 (-311.68, 534.98) Trimming A by 70 70, reference by 80 80 Trimming A by 40 60, reference by 80 80 Rough eucentricity: changing Z by -51.41 to 69.21, continuing... Rough eucentricity: changing Z by -2.09 to 67.12, finished. Z = 15: The original displacements in the overlap zones have mean 22.81 maximum 44.12 pixels. After shifting pieces into register, the displacements have mean 12.61 maximum 12.61 pixels. Running script 6 at item 30 (-212.93, 361.12) Trimming A by 125 130, reference by 135 135 Trimming A by 105 110, reference by 135 135 Rough eucentricity: changing Z by -52.25 to 68.37, continuing... Trimming A by 25 25, reference by 25 25 Trimming A by 20 25, reference by 25 25 Trimming A by 20 25, reference by 20 30 Rough eucentricity: changing Z by -1.69 to 66.68, finished. Z = 16: The original displacements in the overlap zones have mean 15.28 maximum 18.56 pixels. After shifting pieces into register, the displacements have mean 5.20 maximum 5.20 pixels. Running script 6 at item 31 (12.76, 237.55) Trimming A by 105 105, reference by 115 115 Trimming A by 80 80, reference by 115 115 Rough eucentricity: changing Z by -52.85 to 67.77, continuing... Rough eucentricity: changing Z by -1.36 to 66.40, finished. Z = 17: The original displacements in the overlap zones have mean 14.42 maximum 20.89 pixels. After shifting pieces into register, the displacements have mean 5.88 maximum 5.88 pixels. Running script 6 at item 214 (200.09, -21.32) Trimming A by 135 135, reference by 145 145 Trimming A by 110 110, reference by 145 145 Rough eucentricity: changing Z by -61.46 to 59.16, continuing... Rough eucentricity: changing Z by -0.77 to 58.39, finished. Z = 0: The original displacements in the overlap zones have mean 42.18 maximum 102.21 pixels. After shifting pieces into register, the displacements have mean 26.69 maximum 26.69 pixels. Running script 6 at item 215 (26.12, -31.19) Trimming A by 170 170, reference by 180 180 Trimming A by 145 145, reference by 180 180 Trimming A by 95 95, reference by 180 180 Rough eucentricity: changing Z by 67.56 to 188.18, continuing... Trimming A by 110 115, reference by 135 140 Rough eucentricity: changing Z by 57.15 to 245.33, continuing... Trimming A by 20 20, reference by 175 175 Rough eucentricity: changing Z by -62.80 to 182.53, finished. Z = 1: The original displacements in the overlap zones have mean 11.80 maximum 18.67 pixels. After shifting pieces into register, the displacements have mean 5.34 maximum 5.34 pixels. Running script 6 at item 216 (72.68, -73.96) Trimming A by 150 155, reference by 160 165 Trimming A by 130 130, reference by 160 165 Rough eucentricity: changing Z by -61.97 to 58.66, continuing... Trimming A by 25 30, reference by 25 30 Trimming A by 20 30, reference by 25 30 Trimming A by 25 30, reference by 30 30 Rough eucentricity: changing Z by -1.00 to 57.66, finished. Z = 2: The original displacements in the overlap zones have mean 19.51 maximum 28.54 pixels. After shifting pieces into register, the displacements have mean 3.31 maximum 3.31 pixels. Running script 6 at item 217 (113.90, -26.76) Trimming A by 115 115, reference by 130 130 Trimming A by 90 95, reference by 130 130 Rough eucentricity: changing Z by -60.87 to 59.75, continuing... Rough eucentricity: changing Z by -1.02 to 58.73, finished. Z = 3: The original displacements in the overlap zones have mean 17.43 maximum 28.50 pixels. After shifting pieces into register, the displacements have mean 0.25 maximum 0.25 pixels. Running script 6 at item 218 (17.67, 147.31) Trimming A by 115 120, reference by 125 130 Trimming A by 75 80, reference by 125 130 Rough eucentricity: changing Z by -55.37 to 65.25, continuing... Rough eucentricity: changing Z by -1.37 to 63.88, finished. Z = 4: The original displacements in the overlap zones have mean 23.01 maximum 40.06 pixels. After shifting pieces into register, the displacements have mean 15.00 maximum 15.00 pixels. Running script 6 at item 219 (-28.35, 190.86) Trimming A by 130 130, reference by 140 140 Trimming A by 105 105, reference by 140 140 Rough eucentricity: changing Z by -53.98 to 66.63, continuing... Rough eucentricity: changing Z by -1.96 to 64.67, finished. Z = 5: The original displacements in the overlap zones have mean 16.66 maximum 25.62 pixels. After shifting pieces into register, the displacements have mean 9.29 maximum 9.29 pixels. Running script 6 at item 220 (-73.79, 232.96) Trimming A by 130 130, reference by 140 140 Trimming A by 105 105, reference by 140 140 Rough eucentricity: changing Z by -53.85 to 66.77, continuing... Trimming A by 20 20, reference by 20 25 Trimming A by 20 20, reference by 20 25 Trimming A by 20 20, reference by 20 25 Rough eucentricity: changing Z by -1.24 to 65.53, finished. Ending Navigator acquisition after current item Z = 6: The original displacements in the overlap zones have mean 20.65 maximum 24.14 pixels. After shifting pieces into register, the displacements have mean 5.43 maximum 5.43 pixels. Running script 17 at item 48-1 (-887.13, 732.44) Remaining time to fill dewars is 6900 Dewars are NOT busy filling Trimming A by 190 24 160 20, reference by 215 23 20 210 Trimming A by 190 24 160 20, reference by 185 22 155 20 Trimming A by 190 24 160 20, reference by 23 160 20 185 Trimming A by 190 24 160 20, reference by 24 130 120 20 Disparity in stage position after aligning to center of map frame: 0.00 -0.10 Aligning to target with image shift equivalent to stage shift: -0.33 -0.74 Finished aligning to item in 1 rounds Trimming A by 180 20 150 20, reference by 190 24 160 20 -63.9 69.0 unbinned pixels; 337.1 126.1 nm along two shift axes; -255.0 -263.1 nm on specimen axes Total_Shift = 188.064139 Trimming A by 175 20 145 20, reference by 190 24 160 20 -5.7 46.5 unbinned pixels; 178.4 49.4 nm along two shift axes; -179.7 -32.0 nm on specimen axes Total_Shift = 93.686918 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.34 microns changed by -3.14 to target drift = 0.07 nm/sec Measured defocus = -1.89 microns changed by 0.09 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5529.tif 27 frames are being saved to U:\krios\20190717\PilA_5530.tif 27 frames are being saved to U:\krios\20190717\PilA_5531.tif 27 frames are being saved to U:\krios\20190717\PilA_5532.tif 27 frames are being saved to U:\krios\20190717\PilA_5533.tif 27 frames are being saved to U:\krios\20190717\PilA_5534.tif Running script 17 at item 49-1 (-885.77, 729.72) Remaining time to fill dewars is 6720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 20 130 20, reference by 185 22 155 20 Disparity in stage position after aligning to target position: 0.03 -0.08 (2nd move error 0.02 -0.00) Finished aligning to item in 1 rounds Trimming A by 125 20 140 20, reference by 135 20 130 20 -1.0 -1.1 unbinned pixels; 2.9 5.2 nm along two shift axes; 4.6 -3.5 nm on specimen axes Total_Shift = 2.966703 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.12 microns changed by -3.02 to target drift = 0.11 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5535.tif 27 frames are being saved to U:\krios\20190717\PilA_5536.tif 27 frames are being saved to U:\krios\20190717\PilA_5537.tif 27 frames are being saved to U:\krios\20190717\PilA_5538.tif 27 frames are being saved to U:\krios\20190717\PilA_5539.tif 27 frames are being saved to U:\krios\20190717\PilA_5540.tif Running script 17 at item 50-1 (-884.42, 727.02) Remaining time to fill dewars is 6480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 21 125 20, reference by 185 22 155 20 Disparity in stage position after aligning to target position: 0.05 -0.06 (2nd move error 0.05 0.02) Finished aligning to item in 1 rounds Trimming A by 95 20 125 20, reference by 90 21 125 20 0.1 -0.8 unbinned pixels; 3.1 1.1 nm along two shift axes; 3.2 0.4 nm on specimen axes Total_Shift = 1.677571 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.87 microns changed by -2.87 to target drift = 0.12 nm/sec Measured defocus = -2.00 microns changed by -0.00 to target drift = 0.08 nm/sec Ending Navigator acquisition after current item 27 frames are being saved to U:\krios\20190717\PilA_5541.tif 27 frames are being saved to U:\krios\20190717\PilA_5542.tif 27 frames are being saved to U:\krios\20190717\PilA_5543.tif 27 frames are being saved to U:\krios\20190717\PilA_5544.tif 27 frames are being saved to U:\krios\20190717\PilA_5545.tif 27 frames are being saved to U:\krios\20190717\PilA_5546.tif Running script 17 at item 51-1 (-882.77, 724.22) Remaining time to fill dewars is 6180 Dewars are NOT busy filling