Trimming A by 21 121 135 20, reference by 215 23 20 210 Trimming A by 21 121 135 20, reference by 185 22 155 20 Trimming A by 21 121 135 20, reference by 23 160 20 185 Trimming A by 21 121 135 20, reference by 24 130 120 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.12 Trimming A by 20 76 160 20, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.08 -0.01 (2nd move error 0.01 0.09) Finished aligning to item in 2 rounds Trimming A by 24 67 165 20, reference by 20 76 160 20 -2.0 1.1 unbinned pixels; 6.6 5.5 nm along two shift axes; -3.8 -8.0 nm on specimen axes Total_Shift = 4.532195 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.78 microns changed by -2.88 to target drift = 0.18 nm/sec Measured defocus = -2.10 microns changed by -0.00 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5547.tif 27 frames are being saved to U:\krios\20190717\PilA_5548.tif 27 frames are being saved to U:\krios\20190717\PilA_5549.tif 27 frames are being saved to U:\krios\20190717\PilA_5550.tif 27 frames are being saved to U:\krios\20190717\PilA_5551.tif 27 frames are being saved to U:\krios\20190717\PilA_5552.tif Running script 17 at item 52-1 (-883.98, 720.05) Remaining time to fill dewars is 6000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 137 170 20, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.11 -0.07 (2nd move error 0.05 0.03) Finished aligning to item in 1 rounds Trimming A by 21 150 160 20, reference by 20 137 170 20 -1.1 -4.5 unbinned pixels; 15.3 10.7 nm along two shift axes; 17.9 -3.3 nm on specimen axes Total_Shift = 9.33056 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.60 microns changed by -1.60 to target drift = 0.17 nm/sec Measured defocus = -2.16 microns changed by -0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5553.tif 27 frames are being saved to U:\krios\20190717\PilA_5554.tif 27 frames are being saved to U:\krios\20190717\PilA_5555.tif 27 frames are being saved to U:\krios\20190717\PilA_5556.tif 27 frames are being saved to U:\krios\20190717\PilA_5557.tif 27 frames are being saved to U:\krios\20190717\PilA_5558.tif Running script 17 at item 53-1 (-885.40, 722.76) Remaining time to fill dewars is 5820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 76 110 20, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.04 -0.08 (2nd move error -0.02 0.02) Finished aligning to item in 1 rounds Trimming A by 23 76 110 20, reference by 23 76 110 20 3.4 4.4 unbinned pixels; 11.8 18.9 nm along two shift axes; -17.8 12.3 nm on specimen axes Total_Shift = 11.124829 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.63 microns changed by -2.93 to target drift = 0.11 nm/sec Measured defocus = -2.25 microns changed by -0.05 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5559.tif 27 frames are being saved to U:\krios\20190717\PilA_5560.tif 27 frames are being saved to U:\krios\20190717\PilA_5561.tif 27 frames are being saved to U:\krios\20190717\PilA_5562.tif 27 frames are being saved to U:\krios\20190717\PilA_5563.tif 27 frames are being saved to U:\krios\20190717\PilA_5564.tif Running script 17 at item 54-1 (-886.86, 725.54) Remaining time to fill dewars is 5640 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 30 20 55 20, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.08 -0.10 (2nd move error 0.02 0.01) Finished aligning to item in 1 rounds Trimming A by 45 20 45 20, reference by 30 20 55 20 1.3 -1.5 unbinned pixels; 7.1 2.5 nm along two shift axes; 5.4 5.4 nm on specimen axes Total_Shift = 3.933719 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.68 microns changed by -1.72 to target drift = 0.12 nm/sec Measured defocus = -2.34 microns changed by -0.06 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5565.tif 27 frames are being saved to U:\krios\20190717\PilA_5566.tif 27 frames are being saved to U:\krios\20190717\PilA_5567.tif 27 frames are being saved to U:\krios\20190717\PilA_5568.tif 27 frames are being saved to U:\krios\20190717\PilA_5569.tif 27 frames are being saved to U:\krios\20190717\PilA_5570.tif Running script 17 at item 55-1 (-889.82, 731.02) Remaining time to fill dewars is 5460 Dewars are NOT busy filling Trimming A by 190 24 155 20, reference by 215 23 20 210 Trimming A by 190 24 155 20, reference by 185 22 155 20 Trimming A by 190 24 155 20, reference by 23 160 20 185 Trimming A by 190 24 155 20, reference by 24 130 120 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.07 Trimming A by 100 24 85 20, reference by 185 22 155 20 Disparity in stage position after aligning to target position: 0.09 -0.13 (2nd move error -0.04 -0.08) Finished aligning to item in 2 rounds Trimming A by 105 22 80 20, reference by 100 24 85 20 -10.9 8.6 unbinned pixels; 45.8 26.5 nm along two shift axes; -31.0 -44.4 nm on specimen axes Total_Shift = 27.786712 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.64 microns changed by -3.14 to target drift = 0.05 nm/sec Measured defocus = -2.45 microns changed by -0.05 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5571.tif 27 frames are being saved to U:\krios\20190717\PilA_5572.tif 27 frames are being saved to U:\krios\20190717\PilA_5573.tif 27 frames are being saved to U:\krios\20190717\PilA_5574.tif 27 frames are being saved to U:\krios\20190717\PilA_5575.tif 27 frames are being saved to U:\krios\20190717\PilA_5576.tif Running script 17 at item 56-1 (-891.24, 733.77) Remaining time to fill dewars is 5280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 160 20 85 55, reference by 185 22 155 20 Disparity in stage position after aligning to target position: -0.02 -0.09 (2nd move error -0.15 -0.04) Finished aligning to item in 1 rounds Trimming A by 160 20 80 55, reference by 160 20 85 55 -3.1 28.2 unbinned pixels; 107.7 31.2 nm along two shift axes; -109.0 -18.1 nm on specimen axes Total_Shift = 56.730394 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.63 microns changed by -1.97 to target drift = 0.03 nm/sec Measured defocus = -2.66 microns changed by 0.06 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5577.tif 27 frames are being saved to U:\krios\20190717\PilA_5578.tif 27 frames are being saved to U:\krios\20190717\PilA_5579.tif 27 frames are being saved to U:\krios\20190717\PilA_5580.tif 27 frames are being saved to U:\krios\20190717\PilA_5581.tif 27 frames are being saved to U:\krios\20190717\PilA_5582.tif Running script 17 at item 57 (-894.01, 732.17) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Trimming A by 220 20 20 220, reference by 215 23 20 210 Trimming A by 220 20 20 220, reference by 185 22 155 20 Trimming A by 220 20 20 220, reference by 23 160 20 185 Trimming A by 220 20 20 220, reference by 24 130 120 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.03 Trimming A by 145 24 20 115, reference by 215 23 20 210 Disparity in stage position after aligning to target position: 0.31 0.02 (2nd move error 0.22 0.03) Finished aligning to item in 2 rounds Trimming A by 140 20 20 115, reference by 145 24 20 115 1.6 0.2 unbinned pixels; 1.4 6.2 nm along two shift axes; -1.1 6.4 nm on specimen axes Total_Shift = 3.322147 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.50 microns changed by -3.40 to target drift = 0.16 nm/sec Measured defocus = -0.93 microns changed by 0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5583.tif 27 frames are being saved to U:\krios\20190717\PilA_5584.tif 27 frames are being saved to U:\krios\20190717\PilA_5585.tif 27 frames are being saved to U:\krios\20190717\PilA_5586.tif 27 frames are being saved to U:\krios\20190717\PilA_5587.tif 27 frames are being saved to U:\krios\20190717\PilA_5588.tif Running script 17 at item 58 (-892.40, 729.65) Remaining time to fill dewars is 4920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 20 20 60, reference by 185 22 155 20 Disparity in stage position after aligning to target position: 0.04 -0.14 (2nd move error -0.09 -0.09) Finished aligning to item in 1 rounds Trimming A by 65 21 20 60, reference by 70 20 20 60 -6.2 17.8 unbinned pixels; 73.6 4.5 nm along two shift axes; -68.0 -27.8 nm on specimen axes Total_Shift = 37.735727 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.36 microns changed by -3.36 to target drift = 0.08 nm/sec Measured defocus = -1.02 microns changed by 0.02 to target drift = 0.00 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5589.tif 27 frames are being saved to U:\krios\20190717\PilA_5590.tif 27 frames are being saved to U:\krios\20190717\PilA_5591.tif 27 frames are being saved to U:\krios\20190717\PilA_5592.tif 27 frames are being saved to U:\krios\20190717\PilA_5593.tif 27 frames are being saved to U:\krios\20190717\PilA_5594.tif Running script 17 at item 59 (-891.05, 726.95) Remaining time to fill dewars is 4740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 185 22 155 20 Disparity in stage position after aligning to target position: 0.11 -0.12 (2nd move error -0.02 -0.07) Finished aligning to item in 1 rounds -6.6 32.7 unbinned pixels; 128.7 25.4 nm along two shift axes; -125.7 -32.5 nm on specimen axes Total_Shift = 66.640308 3.3 32.0 unbinned pixels; 113.5 60.1 nm along two shift axes; -125.3 6.0 nm on specimen axes Total_Shift = 64.411879 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 2.14 microns changed by -3.24 to target drift = 0.06 nm/sec Measured defocus = -1.13 microns changed by 0.03 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5595.tif 27 frames are being saved to U:\krios\20190717\PilA_5596.tif 27 frames are being saved to U:\krios\20190717\PilA_5597.tif 27 frames are being saved to U:\krios\20190717\PilA_5598.tif 27 frames are being saved to U:\krios\20190717\PilA_5599.tif 27 frames are being saved to U:\krios\20190717\PilA_5600.tif Running script 17 at item 60 (-889.50, 724.12) Remaining time to fill dewars is 4560 Dewars are NOT busy filling Trimming A by 22 135 120 20, reference by 215 23 20 210 Trimming A by 22 135 120 20, reference by 185 22 155 20 Trimming A by 22 135 120 20, reference by 23 160 20 185 Trimming A by 22 135 120 20, reference by 24 130 120 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.10 Trimming A by 20 20 20 20, reference by 24 130 120 20 Disparity in stage position after aligning to target position: -0.01 -0.12 (2nd move error -0.12 -0.04) Finished aligning to item in 2 rounds -3.2 22.8 unbinned pixels; 88.1 22.8 nm along two shift axes; -88.1 -17.3 nm on specimen axes Total_Shift = 46.079896 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 2.02 microns changed by -3.22 to target drift = 0.08 nm/sec Measured defocus = -1.20 microns changed by -0.00 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5601.tif 27 frames are being saved to U:\krios\20190717\PilA_5602.tif 27 frames are being saved to U:\krios\20190717\PilA_5603.tif 27 frames are being saved to U:\krios\20190717\PilA_5604.tif 27 frames are being saved to U:\krios\20190717\PilA_5605.tif 27 frames are being saved to U:\krios\20190717\PilA_5606.tif Running script 17 at item 61 (-888.05, 721.33) Remaining time to fill dewars is 4440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 70 70 20, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.01 -0.13 (2nd move error -0.11 -0.06) Finished aligning to item in 1 rounds Trimming A by 24 70 70 20, reference by 24 70 70 20 -5.6 22.4 unbinned pixels; 89.6 13.5 nm along two shift axes; -85.9 -26.5 nm on specimen axes Total_Shift = 46.135962 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.87 microns changed by -3.17 to target drift = 0.08 nm/sec Measured defocus = -1.30 microns changed by -0.00 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5607.tif 27 frames are being saved to U:\krios\20190717\PilA_5608.tif 27 frames are being saved to U:\krios\20190717\PilA_5609.tif 27 frames are being saved to U:\krios\20190717\PilA_5610.tif 27 frames are being saved to U:\krios\20190717\PilA_5611.tif 27 frames are being saved to U:\krios\20190717\PilA_5612.tif Running script 17 at item 62 (-886.61, 718.61) Remaining time to fill dewars is 4200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 145 120 20, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.17 -0.11 (2nd move error 0.06 -0.01) Finished aligning to item in 1 rounds Trimming A by 22 145 120 20, reference by 22 145 120 20 -1.6 3.2 unbinned pixels; 14.0 1.0 nm along two shift axes; -12.3 -7.0 nm on specimen axes Total_Shift = 7.247021 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.69 microns changed by -3.09 to target drift = 0.09 nm/sec Measured defocus = -1.39 microns changed by -0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5613.tif 27 frames are being saved to U:\krios\20190717\PilA_5614.tif 27 frames are being saved to U:\krios\20190717\PilA_5615.tif 27 frames are being saved to U:\krios\20190717\PilA_5616.tif 27 frames are being saved to U:\krios\20190717\PilA_5617.tif 27 frames are being saved to U:\krios\20190717\PilA_5618.tif Running script 17 at item 63 (-889.27, 717.15) Remaining time to fill dewars is 4080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 130 85 25, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.14 -0.15 (2nd move error 0.03 -0.07) Finished aligning to item in 1 rounds Trimming A by 21 135 85 20, reference by 20 130 85 25 -9.9 0.4 unbinned pixels; 14.4 35.0 nm along two shift axes; 0.5 -38.5 nm on specimen axes Total_Shift = 19.783571 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.70 microns changed by -3.20 to target drift = 0.09 nm/sec Measured defocus = -1.51 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5619.tif 27 frames are being saved to U:\krios\20190717\PilA_5620.tif 27 frames are being saved to U:\krios\20190717\PilA_5621.tif 27 frames are being saved to U:\krios\20190717\PilA_5622.tif 27 frames are being saved to U:\krios\20190717\PilA_5623.tif 27 frames are being saved to U:\krios\20190717\PilA_5624.tif Running script 17 at item 64 (-890.72, 719.95) Remaining time to fill dewars is 3840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 55 35 45, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.12 -0.18 (2nd move error 0.00 -0.10) Finished aligning to item in 1 rounds Trimming A by 20 55 35 45, reference by 20 55 35 45 -13.5 0.7 unbinned pixels; 20.1 47.8 nm along two shift axes; 0.3 -52.8 nm on specimen axes Total_Shift = 27.12584 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.65 microns changed by -3.25 to target drift = 0.06 nm/sec Measured defocus = -1.58 microns changed by -0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5625.tif 27 frames are being saved to U:\krios\20190717\PilA_5626.tif 27 frames are being saved to U:\krios\20190717\PilA_5627.tif 27 frames are being saved to U:\krios\20190717\PilA_5628.tif 27 frames are being saved to U:\krios\20190717\PilA_5629.tif 27 frames are being saved to U:\krios\20190717\PilA_5630.tif Running script 17 at item 65 (-892.15, 722.72) Remaining time to fill dewars is 3720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.02 -0.15 (2nd move error -0.10 -0.07) Finished aligning to item in 1 rounds -6.3 18.9 unbinned pixels; 77.8 5.8 nm along two shift axes; -72.2 -28.5 nm on specimen axes Total_Shift = 39.864043 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.62 microns changed by -3.32 to target drift = 0.08 nm/sec Measured defocus = -1.72 microns changed by 0.02 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5631.tif 27 frames are being saved to U:\krios\20190717\PilA_5632.tif 27 frames are being saved to U:\krios\20190717\PilA_5633.tif 27 frames are being saved to U:\krios\20190717\PilA_5634.tif 27 frames are being saved to U:\krios\20190717\PilA_5635.tif 27 frames are being saved to U:\krios\20190717\PilA_5636.tif Running script 17 at item 66 (-893.55, 725.26) Remaining time to fill dewars is 3480 Dewars are NOT busy filling Trimming A by 20 144 20 210, reference by 215 23 20 210 Trimming A by 20 144 20 210, reference by 185 22 155 20 Trimming A by 20 144 20 210, reference by 23 160 20 185 Trimming A by 20 144 20 210, reference by 24 130 120 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.09 Trimming A by 20 20 20 20, reference by 23 160 20 185 Disparity in stage position after aligning to target position: 0.09 0.08 (2nd move error -0.05 0.15) Finished aligning to item in 2 rounds 0.3 11.8 unbinned pixels; 43.0 18.9 nm along two shift axes; -46.0 -1.3 nm on specimen axes Total_Shift = 23.63357 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.18 microns changed by -1.98 to target drift = 0.13 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5637.tif 27 frames are being saved to U:\krios\20190717\PilA_5638.tif 27 frames are being saved to U:\krios\20190717\PilA_5639.tif 27 frames are being saved to U:\krios\20190717\PilA_5640.tif 27 frames are being saved to U:\krios\20190717\PilA_5641.tif 27 frames are being saved to U:\krios\20190717\PilA_5642.tif Running script 17 at item 67 (-895.28, 728.02) Remaining time to fill dewars is 3300 Dewars are NOT busy filling Trimming A by 220 20 20 220, reference by 215 23 20 210 Trimming A by 220 20 20 220, reference by 185 22 155 20 Trimming A by 220 20 20 220, reference by 23 160 20 185 Trimming A by 220 20 20 220, reference by 24 130 120 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.07 Trimming A by 80 20 20 85, reference by 215 23 20 210 Disparity in stage position after aligning to target position: 0.38 -0.04 (2nd move error 0.24 0.01) Finished aligning to item in 2 rounds Trimming A by 80 20 20 85, reference by 80 20 20 85 2.4 8.1 unbinned pixels; 26.5 21.0 nm along two shift axes; -32.0 7.8 nm on specimen axes Total_Shift = 16.899838 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.56 microns changed by -3.46 to target drift = 0.13 nm/sec Measured defocus = -1.95 microns changed by 0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5643.tif 27 frames are being saved to U:\krios\20190717\PilA_5644.tif 27 frames are being saved to U:\krios\20190717\PilA_5645.tif 27 frames are being saved to U:\krios\20190717\PilA_5646.tif 27 frames are being saved to U:\krios\20190717\PilA_5647.tif 27 frames are being saved to U:\krios\20190717\PilA_5648.tif Running script 17 at item 68 (-897.84, 726.57) Remaining time to fill dewars is 3060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 82 23 20 140, reference by 215 23 20 210 Disparity in stage position after aligning to target position: 0.36 -0.09 (2nd move error 0.23 -0.05) Finished aligning to item in 1 rounds Trimming A by 81 20 20 130, reference by 82 23 20 140 -3.4 4.3 unbinned pixels; 20.1 5.9 nm along two shift axes; -15.8 -14.3 nm on specimen axes Total_Shift = 10.940253 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.19 microns changed by -2.19 to target drift = 0.09 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5649.tif 27 frames are being saved to U:\krios\20190717\PilA_5650.tif 27 frames are being saved to U:\krios\20190717\PilA_5651.tif 27 frames are being saved to U:\krios\20190717\PilA_5652.tif 27 frames are being saved to U:\krios\20190717\PilA_5653.tif 27 frames are being saved to U:\krios\20190717\PilA_5654.tif Running script 17 at item 69 (-896.15, 723.77) Remaining time to fill dewars is 2940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 40 20 85, reference by 23 160 20 185 Disparity in stage position after aligning to target position: 0.12 -0.03 (2nd move error -0.03 0.03) Finished aligning to item in 1 rounds Trimming A by 21 32 20 90, reference by 22 40 20 85 -0.5 23.0 unbinned pixels; 85.1 32.8 nm along two shift axes; -89.3 -6.7 nm on specimen axes Total_Shift = 45.963231 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 1.37 microns changed by -3.47 to target drift = 0.08 nm/sec Measured defocus = -2.21 microns changed by 0.11 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5655.tif 27 frames are being saved to U:\krios\20190717\PilA_5656.tif 27 frames are being saved to U:\krios\20190717\PilA_5657.tif 27 frames are being saved to U:\krios\20190717\PilA_5658.tif 27 frames are being saved to U:\krios\20190717\PilA_5659.tif 27 frames are being saved to U:\krios\20190717\PilA_5660.tif Running script 17 at item 70 (-894.73, 721.04) Remaining time to fill dewars is 2760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 20 90, reference by 23 160 20 185 Disparity in stage position after aligning to target position: 0.16 0.00 (2nd move error 0.01 0.07) Finished aligning to item in 1 rounds Trimming A by 20 80 20 85, reference by 20 80 20 90 0.1 -0.1 unbinned pixels; 0.5 0.1 nm along two shift axes; 0.4 0.3 nm on specimen axes Total_Shift = 0.253225 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.19 microns changed by -2.01 to target drift = 0.12 nm/sec Measured defocus = -2.23 microns changed by 0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5661.tif 27 frames are being saved to U:\krios\20190717\PilA_5662.tif 27 frames are being saved to U:\krios\20190717\PilA_5663.tif 27 frames are being saved to U:\krios\20190717\PilA_5664.tif 27 frames are being saved to U:\krios\20190717\PilA_5665.tif 27 frames are being saved to U:\krios\20190717\PilA_5666.tif Running script 17 at item 71 (-893.32, 718.23) Remaining time to fill dewars is 2640 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 120 20 110, reference by 23 160 20 185 Disparity in stage position after aligning to target position: 0.21 0.04 (2nd move error 0.06 0.11) Finished aligning to item in 1 rounds Trimming A by 22 120 20 105, reference by 22 120 20 110 1.3 -0.5 unbinned pixels; 3.6 4.0 nm along two shift axes; 1.7 5.3 nm on specimen axes Total_Shift = 2.852091 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 1.03 microns changed by -3.33 to target drift = 0.15 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5667.tif 27 frames are being saved to U:\krios\20190717\PilA_5668.tif 27 frames are being saved to U:\krios\20190717\PilA_5669.tif 27 frames are being saved to U:\krios\20190717\PilA_5670.tif 27 frames are being saved to U:\krios\20190717\PilA_5671.tif 27 frames are being saved to U:\krios\20190717\PilA_5672.tif Running script 17 at item 72 (-891.89, 715.78) Remaining time to fill dewars is 2400 Dewars are NOT busy filling Trimming A by 22 135 120 20, reference by 215 23 20 210 Trimming A by 22 135 120 20, reference by 185 22 155 20 Trimming A by 22 135 120 20, reference by 23 160 20 185 Trimming A by 22 135 120 20, reference by 24 130 120 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.07 Trimming A by 23 160 20 115, reference by 24 130 120 20 Disparity in stage position after aligning to target position: 0.13 -0.19 (2nd move error -0.02 -0.13) Finished aligning to item in 2 rounds Trimming A by 22 165 20 110, reference by 23 160 20 115 -16.0 6.0 unbinned pixels; 43.1 48.6 nm along two shift axes; -20.1 -63.6 nm on specimen axes Total_Shift = 34.260644 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.46 microns changed by -1.94 to target drift = 0.06 nm/sec Measured defocus = -2.36 microns changed by -0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5673.tif 27 frames are being saved to U:\krios\20190717\PilA_5674.tif 27 frames are being saved to U:\krios\20190717\PilA_5675.tif 27 frames are being saved to U:\krios\20190717\PilA_5676.tif 27 frames are being saved to U:\krios\20190717\PilA_5677.tif 27 frames are being saved to U:\krios\20190717\PilA_5678.tif Running script 17 at item 73 (-703.34, 648.01) Remaining time to fill dewars is 2220 Dewars are NOT busy filling Trimming A by 171 23 175 20, reference by 180 22 20 185 Trimming A by 171 23 175 20, reference by 162 23 170 20 Trimming A by 171 23 175 20, reference by 24 166 20 200 Trimming A by 171 23 175 20, reference by 20 170 145 20 Disparity in stage position after aligning to center of map frame: 0.02 -0.13 Trimming A by 125 20 155 20, reference by 162 23 170 20 Disparity in stage position after aligning to target position: 0.21 -0.10 (2nd move error 0.19 0.01) Finished aligning to item in 2 rounds Trimming A by 120 23 165 20, reference by 125 20 155 20 2.6 6.9 unbinned pixels; 22.1 19.7 nm along two shift axes; -27.5 8.5 nm on specimen axes Total_Shift = 14.772769 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.35 microns changed by -2.15 to target drift = 0.24 nm/sec Measured defocus = -2.49 microns changed by -0.01 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5679.tif 27 frames are being saved to U:\krios\20190717\PilA_5680.tif 27 frames are being saved to U:\krios\20190717\PilA_5681.tif 27 frames are being saved to U:\krios\20190717\PilA_5682.tif 27 frames are being saved to U:\krios\20190717\PilA_5683.tif 27 frames are being saved to U:\krios\20190717\PilA_5684.tif Running script 17 at item 74 (-708.81, 652.00) Remaining time to fill dewars is 2160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 136 22 110 20, reference by 162 23 170 20 Disparity in stage position after aligning to target position: 0.01 -0.19 (2nd move error -0.01 -0.09) Finished aligning to item in 1 rounds Trimming A by 145 21 100 20, reference by 136 22 110 20 -12.1 3.5 unbinned pixels; 28.9 38.4 nm along two shift axes; -11.2 -48.0 nm on specimen axes Total_Shift = 25.288214 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.42 microns changed by -2.18 to target drift = 0.03 nm/sec Measured defocus = -2.60 microns changed by -0.00 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5685.tif 27 frames are being saved to U:\krios\20190717\PilA_5686.tif 27 frames are being saved to U:\krios\20190717\PilA_5687.tif 27 frames are being saved to U:\krios\20190717\PilA_5688.tif 27 frames are being saved to U:\krios\20190717\PilA_5689.tif 27 frames are being saved to U:\krios\20190717\PilA_5690.tif Running script 17 at item 75 (-707.42, 649.24) Remaining time to fill dewars is 1920 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 95 24 95 20, reference by 162 23 170 20 Disparity in stage position after aligning to target position: 0.11 -0.19 (2nd move error 0.09 -0.08) Finished aligning to item in 1 rounds Trimming A by 95 24 95 20, reference by 95 24 95 20 -7.6 -5.6 unbinned pixels; 10.7 36.0 nm along two shift axes; 23.5 -28.6 nm on specimen axes Total_Shift = 18.986576 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.40 microns changed by -2.30 to target drift = 0.11 nm/sec Measured defocus = -1.09 microns changed by 0.19 to target drift = 0.23 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5691.tif 27 frames are being saved to U:\krios\20190717\PilA_5692.tif 27 frames are being saved to U:\krios\20190717\PilA_5693.tif 27 frames are being saved to U:\krios\20190717\PilA_5694.tif 27 frames are being saved to U:\krios\20190717\PilA_5695.tif 27 frames are being saved to U:\krios\20190717\PilA_5696.tif Running script 17 at item 76 (-705.98, 646.55) Remaining time to fill dewars is 1800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 20 75 20, reference by 162 23 170 20 Disparity in stage position after aligning to target position: 0.22 -0.22 (2nd move error 0.20 -0.10) Finished aligning to item in 1 rounds Trimming A by 70 20 80 20, reference by 70 20 75 20 -8.8 6.3 unbinned pixels; 34.7 22.0 nm along two shift axes; -22.8 -35.4 nm on specimen axes Total_Shift = 21.624071 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.30 microns changed by -2.30 to target drift = 0.06 nm/sec Measured defocus = -1.00 microns changed by 0.00 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5697.tif 27 frames are being saved to U:\krios\20190717\PilA_5698.tif 27 frames are being saved to U:\krios\20190717\PilA_5699.tif 27 frames are being saved to U:\krios\20190717\PilA_5700.tif 27 frames are being saved to U:\krios\20190717\PilA_5701.tif 27 frames are being saved to U:\krios\20190717\PilA_5702.tif Running script 17 at item 77 (-704.37, 643.85) Remaining time to fill dewars is 1500 Dewars are NOT busy filling Ending Navigator acquisition after current item Trimming A by 23 165 150 20, reference by 180 22 20 185 Trimming A by 23 165 150 20, reference by 162 23 170 20 Trimming A by 23 165 150 20, reference by 24 166 20 200 Trimming A by 23 165 150 20, reference by 20 170 145 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.19 Trimming A by 21 65 100 20, reference by 20 170 145 20 Disparity in stage position after aligning to target position: 0.10 -0.16 (2nd move error -0.04 0.01) Finished aligning to item in 2 rounds Trimming A by 23 60 105 20, reference by 21 65 100 20 1.8 9.1 unbinned pixels; 31.1 20.0 nm along two shift axes; -35.7 5.0 nm on specimen axes Total_Shift = 18.509815 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.11 microns changed by -2.21 to target drift = 0.14 nm/sec Measured defocus = -1.05 microns changed by -0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5703.tif 27 frames are being saved to U:\krios\20190717\PilA_5704.tif 27 frames are being saved to U:\krios\20190717\PilA_5705.tif 27 frames are being saved to U:\krios\20190717\PilA_5706.tif 27 frames are being saved to U:\krios\20190717\PilA_5707.tif 27 frames are being saved to U:\krios\20190717\PilA_5708.tif Running script 17 at item 78 (-702.94, 641.09) Remaining time to fill dewars is 1320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 135 155 20, reference by 20 170 145 20 Disparity in stage position after aligning to target position: 0.16 -0.15 (2nd move error 0.03 0.03) Finished aligning to item in 1 rounds Trimming A by 23 135 155 20, reference by 23 135 155 20 0.1 -0.9 unbinned pixels; 3.5 1.0 nm along two shift axes; 3.6 0.6 nm on specimen axes Total_Shift = 1.860374 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 0.99 microns changed by -2.19 to target drift = 0.17 nm/sec Measured defocus = -1.14 microns changed by -0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5709.tif 27 frames are being saved to U:\krios\20190717\PilA_5710.tif 27 frames are being saved to U:\krios\20190717\PilA_5711.tif 27 frames are being saved to U:\krios\20190717\PilA_5712.tif 27 frames are being saved to U:\krios\20190717\PilA_5713.tif 27 frames are being saved to U:\krios\20190717\PilA_5714.tif Running script 17 at item 79 (-705.59, 639.60) Remaining time to fill dewars is 1140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 125 115 20, reference by 20 170 145 20 Disparity in stage position after aligning to target position: 0.13 -0.22 (2nd move error 0.00 -0.05) Finished aligning to item in 1 rounds Trimming A by 20 125 115 20, reference by 20 125 115 20 -6.5 0.2 unbinned pixels; 9.4 23.2 nm along two shift axes; 0.5 -25.5 nm on specimen axes Total_Shift = 13.080745 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.02 microns changed by -2.32 to target drift = 0.07 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5715.tif 27 frames are being saved to U:\krios\20190717\PilA_5716.tif 27 frames are being saved to U:\krios\20190717\PilA_5717.tif 27 frames are being saved to U:\krios\20190717\PilA_5718.tif 27 frames are being saved to U:\krios\20190717\PilA_5719.tif 27 frames are being saved to U:\krios\20190717\PilA_5720.tif Running script 17 at item 80 (-707.03, 642.39) Remaining time to fill dewars is 1080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 55 55 20, reference by 20 170 145 20 Disparity in stage position after aligning to target position: 0.12 -0.27 (2nd move error -0.01 -0.10) Finished aligning to item in 1 rounds Trimming A by 20 55 55 20, reference by 20 55 55 20 -14.0 4.2 unbinned pixels; 33.6 44.1 nm along two shift axes; -13.3 -55.3 nm on specimen axes Total_Shift = 29.195167 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.89 microns changed by -2.29 to target drift = 0.04 nm/sec Measured defocus = -1.39 microns changed by -0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5721.tif 27 frames are being saved to U:\krios\20190717\PilA_5722.tif 27 frames are being saved to U:\krios\20190717\PilA_5723.tif 27 frames are being saved to U:\krios\20190717\PilA_5724.tif 27 frames are being saved to U:\krios\20190717\PilA_5725.tif 27 frames are being saved to U:\krios\20190717\PilA_5726.tif Running script 17 at item 81 (-708.59, 645.18) Remaining time to fill dewars is 840 Dewars are NOT busy filling Trimming A by 167 21 175 20, reference by 180 22 20 185 Trimming A by 167 21 175 20, reference by 162 23 170 20 Trimming A by 167 21 175 20, reference by 24 166 20 200 Trimming A by 167 21 175 20, reference by 20 170 145 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.14 Trimming A by 20 20 20 20, reference by 162 23 170 20 Disparity in stage position after aligning to target position: 0.17 -0.25 (2nd move error 0.01 -0.13) Finished aligning to item in 2 rounds -17.7 0.7 unbinned pixels; 25.6 62.6 nm along two shift axes; 1.0 -68.9 nm on specimen axes Total_Shift = 35.392166 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.76 microns changed by -2.26 to target drift = 0.10 nm/sec Measured defocus = -1.47 microns changed by -0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5727.tif 27 frames are being saved to U:\krios\20190717\PilA_5728.tif 27 frames are being saved to U:\krios\20190717\PilA_5729.tif 27 frames are being saved to U:\krios\20190717\PilA_5730.tif 27 frames are being saved to U:\krios\20190717\PilA_5731.tif 27 frames are being saved to U:\krios\20190717\PilA_5732.tif Running script 17 at item 82 (-709.95, 647.90) Remaining time to fill dewars is 540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 40 20 30 20, reference by 162 23 170 20 Disparity in stage position after aligning to target position: 0.20 -0.26 (2nd move error 0.04 -0.14) Finished aligning to item in 1 rounds Trimming A by 40 20 30 20, reference by 40 20 30 20 -17.8 -0.8 unbinned pixels; 20.2 65.5 nm along two shift axes; 7.1 -69.3 nm on specimen axes Total_Shift = 35.7351 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.73 microns changed by -2.33 to target drift = 0.10 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5733.tif 27 frames are being saved to U:\krios\20190717\PilA_5734.tif 27 frames are being saved to U:\krios\20190717\PilA_5735.tif 27 frames are being saved to U:\krios\20190717\PilA_5736.tif 27 frames are being saved to U:\krios\20190717\PilA_5737.tif 27 frames are being saved to U:\krios\20190717\PilA_5738.tif Running script 17 at item 83 (-711.50, 650.41) Remaining time to fill dewars is 540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 20 25 70, reference by 162 23 170 20 Disparity in stage position after aligning to target position: 0.07 -0.32 (2nd move error -0.08 -0.20) Finished aligning to item in 1 rounds Trimming A by 85 20 25 70, reference by 90 20 25 70 -19.3 18.0 unbinned pixels; 91.4 42.2 nm along two shift axes; -66.0 -78.7 nm on specimen axes Total_Shift = 52.7297 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.61 microns changed by -2.31 to target drift = 0.01 nm/sec Measured defocus = -1.70 microns changed by 0.00 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5739.tif 27 frames are being saved to U:\krios\20190717\PilA_5740.tif 27 frames are being saved to U:\krios\20190717\PilA_5741.tif 27 frames are being saved to U:\krios\20190717\PilA_5742.tif 27 frames are being saved to U:\krios\20190717\PilA_5743.tif 27 frames are being saved to U:\krios\20190717\PilA_5744.tif Running script 17 at item 84 (-714.12, 648.86) Remaining time to fill dewars is 300 Dewars are NOT busy filling Trimming A by 177 21 20 205, reference by 180 22 20 185 Trimming A by 177 21 20 205, reference by 162 23 170 20 Trimming A by 177 21 20 205, reference by 24 166 20 200 Trimming A by 177 21 20 205, reference by 20 170 145 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.07 Trimming A by 95 24 20 95, reference by 180 22 20 185 Disparity in stage position after aligning to target position: 0.34 0.00 (2nd move error 0.17 0.06) Finished aligning to item in 2 rounds Trimming A by 95 24 20 95, reference by 95 24 20 95 0.5 1.6 unbinned pixels; 5.2 4.4 nm along two shift axes; -6.4 1.8 nm on specimen axes Total_Shift = 3.399311 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.59 microns changed by -2.39 to target drift = 0.14 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5745.tif 27 frames are being saved to U:\krios\20190717\PilA_5746.tif 27 frames are being saved to U:\krios\20190717\PilA_5747.tif 27 frames are being saved to U:\krios\20190717\PilA_5748.tif 27 frames are being saved to U:\krios\20190717\PilA_5749.tif 27 frames are being saved to U:\krios\20190717\PilA_5750.tif Running script 17 at item 85 (-712.78, 646.20) Remaining time to fill dewars is 180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 31 20 20 30, reference by 180 22 20 185 Disparity in stage position after aligning to target position: 0.37 -0.08 (2nd move error 0.21 -0.02) Finished aligning to item in 1 rounds Trimming A by 36 20 20 20, reference by 31 20 20 30 0.2 -3.0 unbinned pixels; 11.3 3.9 nm along two shift axes; 11.7 1.3 nm on specimen axes Total_Shift = 6.049831 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Using peak at -5.9,-11.1, rejecting 1.52 times higher peak at 0.2,-0.1 Measured defocus = 0.40 microns changed by -2.30 to target drift = 0.15 nm/sec Measured defocus = -1.82 microns changed by -0.08 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5751.tif 27 frames are being saved to U:\krios\20190717\PilA_5752.tif 27 frames are being saved to U:\krios\20190717\PilA_5753.tif 27 frames are being saved to U:\krios\20190717\PilA_5754.tif 27 frames are being saved to U:\krios\20190717\PilA_5755.tif 27 frames are being saved to U:\krios\20190717\PilA_5756.tif Running script 17 at item 86 (-711.08, 643.70) Remaining time to fill dewars is 300 Dewars ARE busy filling Dewars ARE busy filling Dewars are NOT busy filling Trimming A by 20 170 145 20, reference by 180 22 20 185 Trimming A by 20 170 145 20, reference by 162 23 170 20 Trimming A by 20 170 145 20, reference by 24 166 20 200 Trimming A by 20 170 145 20, reference by 20 170 145 20 Disparity in stage position after aligning to center of map frame: 0.19 -0.12 Trimming A by 20 20 20 20, reference by 20 170 145 20 Disparity in stage position after aligning to target position: -0.03 -0.28 (2nd move error -0.22 -0.17) Finished aligning to item in 2 rounds -14.8 43.5 unbinned pixels; 179.3 12.3 nm along two shift axes; -166.1 -66.7 nm on specimen axes Total_Shift = 91.898126 -1.9 28.5 unbinned pixels; 107.4 36.2 nm along two shift axes; -110.6 -13.3 nm on specimen axes Total_Shift = 57.196664 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.36 microns changed by -2.36 to target drift = 0.01 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5757.tif 27 frames are being saved to U:\krios\20190717\PilA_5758.tif 27 frames are being saved to U:\krios\20190717\PilA_5759.tif 27 frames are being saved to U:\krios\20190717\PilA_5760.tif 27 frames are being saved to U:\krios\20190717\PilA_5761.tif 27 frames are being saved to U:\krios\20190717\PilA_5762.tif Running script 17 at item 87 (-709.65, 640.99) Remaining time to fill dewars is 31560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 40 25 20, reference by 20 170 145 20 Disparity in stage position after aligning to target position: 0.11 -0.28 (2nd move error -0.08 -0.15) Finished aligning to item in 1 rounds Trimming A by 20 40 25 20, reference by 20 40 25 20 -16.9 15.5 unbinned pixels; 78.9 37.4 nm along two shift axes; -56.7 -68.8 nm on specimen axes Total_Shift = 45.768804 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.18 microns changed by -2.28 to target drift = 0.04 nm/sec Measured defocus = -2.11 microns changed by 0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5763.tif 27 frames are being saved to U:\krios\20190717\PilA_5764.tif 27 frames are being saved to U:\krios\20190717\PilA_5765.tif 27 frames are being saved to U:\krios\20190717\PilA_5766.tif 27 frames are being saved to U:\krios\20190717\PilA_5767.tif 27 frames are being saved to U:\krios\20190717\PilA_5768.tif Running script 17 at item 88 (-708.21, 638.23) Remaining time to fill dewars is 31440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 115 75 20, reference by 20 170 145 20 Disparity in stage position after aligning to target position: 0.10 -0.23 (2nd move error -0.10 -0.12) Finished aligning to item in 1 rounds Trimming A by 20 115 80 20, reference by 20 115 75 20 -13.7 19.4 unbinned pixels; 89.3 20.0 nm along two shift axes; -72.7 -57.4 nm on specimen axes Total_Shift = 47.535058 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.11 microns changed by -2.31 to target drift = 0.05 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5769.tif 27 frames are being saved to U:\krios\20190717\PilA_5770.tif 27 frames are being saved to U:\krios\20190717\PilA_5771.tif 27 frames are being saved to U:\krios\20190717\PilA_5772.tif 27 frames are being saved to U:\krios\20190717\PilA_5773.tif 27 frames are being saved to U:\krios\20190717\PilA_5774.tif Running script 17 at item 89 (-710.87, 636.55) Remaining time to fill dewars is 31380 Dewars are NOT busy filling Trimming A by 23 180 20 200, reference by 180 22 20 185 Trimming A by 23 180 20 200, reference by 162 23 170 20 Trimming A by 23 180 20 200, reference by 24 166 20 200 Trimming A by 23 180 20 200, reference by 20 170 145 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.12 Trimming A by 20 145 20 100, reference by 24 166 20 200 Disparity in stage position after aligning to target position: 0.34 0.10 (2nd move error 0.18 0.20) Finished aligning to item in 2 rounds Trimming A by 22 135 20 95, reference by 20 145 20 100 0.6 -3.8 unbinned pixels; 14.8 3.5 nm along two shift axes; 14.7 3.3 nm on specimen axes Total_Shift = 7.726648 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.04 microns changed by -2.34 to target drift = 0.19 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5775.tif 27 frames are being saved to U:\krios\20190717\PilA_5776.tif 27 frames are being saved to U:\krios\20190717\PilA_5777.tif 27 frames are being saved to U:\krios\20190717\PilA_5778.tif 27 frames are being saved to U:\krios\20190717\PilA_5779.tif 27 frames are being saved to U:\krios\20190717\PilA_5780.tif Running script 17 at item 90 (-712.41, 639.27) Remaining time to fill dewars is 31260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 77 20 105, reference by 24 166 20 200 Disparity in stage position after aligning to target position: 0.27 0.01 (2nd move error 0.11 0.11) Finished aligning to item in 1 rounds Trimming A by 20 76 20 100, reference by 22 77 20 105 -0.1 -0.9 unbinned pixels; 3.1 1.6 nm along two shift axes; 3.4 -0.1 nm on specimen axes Total_Shift = 1.724456 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Using peak at -4.6,-3.9, rejecting 1.12 times higher peak at -0.1,-0.2 Using peak at -3.2,-5.8, rejecting 1.52 times higher peak at 0.1,-0.0 Measured defocus = -0.01 microns changed by -2.39 to target drift = 0.14 nm/sec Measured defocus = -2.45 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5781.tif 27 frames are being saved to U:\krios\20190717\PilA_5782.tif 27 frames are being saved to U:\krios\20190717\PilA_5783.tif 27 frames are being saved to U:\krios\20190717\PilA_5784.tif 27 frames are being saved to U:\krios\20190717\PilA_5785.tif 27 frames are being saved to U:\krios\20190717\PilA_5786.tif Running script 17 at item 91 (-713.78, 641.99) Remaining time to fill dewars is 31140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 42 20 90, reference by 24 166 20 200 Disparity in stage position after aligning to target position: 0.22 -0.09 (2nd move error 0.06 0.01) Finished aligning to item in 1 rounds Trimming A by 22 50 20 80, reference by 22 42 20 90 1.2 -1.5 unbinned pixels; 6.9 2.1 nm along two shift axes; 5.4 4.9 nm on specimen axes Total_Shift = 3.759859 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.20 microns changed by -2.30 to target drift = 0.14 nm/sec Measured defocus = -2.46 microns changed by -0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5787.tif 27 frames are being saved to U:\krios\20190717\PilA_5788.tif 27 frames are being saved to U:\krios\20190717\PilA_5789.tif 27 frames are being saved to U:\krios\20190717\PilA_5790.tif 27 frames are being saved to U:\krios\20190717\PilA_5791.tif 27 frames are being saved to U:\krios\20190717\PilA_5792.tif Running script 17 at item 92 (-715.26, 644.85) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Trimming A by 190 22 20 195, reference by 180 22 20 185 Trimming A by 190 22 20 195, reference by 162 23 170 20 Trimming A by 190 22 20 195, reference by 24 166 20 200 Trimming A by 190 22 20 195, reference by 20 170 145 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.09 Trimming A by 31 21 20 85, reference by 180 22 20 185 Disparity in stage position after aligning to target position: 0.40 -0.06 (2nd move error 0.22 0.00) Finished aligning to item in 2 rounds Trimming A by 45 21 20 65, reference by 31 21 20 85 1.8 0.3 unbinned pixels; 1.2 6.9 nm along two shift axes; -1.6 6.9 nm on specimen axes Total_Shift = 3.64867 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.23 microns changed by -2.37 to target drift = 0.14 nm/sec Measured defocus = -2.64 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5793.tif 27 frames are being saved to U:\krios\20190717\PilA_5794.tif 27 frames are being saved to U:\krios\20190717\PilA_5795.tif 27 frames are being saved to U:\krios\20190717\PilA_5796.tif 27 frames are being saved to U:\krios\20190717\PilA_5797.tif 27 frames are being saved to U:\krios\20190717\PilA_5798.tif Running script 17 at item 93 (-716.78, 647.46) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 110 20 20 130, reference by 180 22 20 185 Disparity in stage position after aligning to target position: 0.26 -0.10 (2nd move error 0.07 -0.03) Finished aligning to item in 1 rounds Trimming A by 79 23 20 150, reference by 110 20 20 130 -3.4 -3.0 unbinned pixels; 6.6 16.9 nm along two shift axes; 12.4 -12.7 nm on specimen axes Total_Shift = 9.120737 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 0.26 microns changed by -1.16 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5799.tif 27 frames are being saved to U:\krios\20190717\PilA_5800.tif 27 frames are being saved to U:\krios\20190717\PilA_5801.tif 27 frames are being saved to U:\krios\20190717\PilA_5802.tif 27 frames are being saved to U:\krios\20190717\PilA_5803.tif 27 frames are being saved to U:\krios\20190717\PilA_5804.tif Running script 17 at item 94 (-716.40, 640.61) Remaining time to fill dewars is 30840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 102 20 155, reference by 24 166 20 200 Disparity in stage position after aligning to target position: 0.15 -0.12 (2nd move error -0.01 -0.01) Finished aligning to item in 1 rounds Trimming A by 23 102 20 155, reference by 23 102 20 155 -2.5 2.9 unbinned pixels; 14.1 4.5 nm along two shift axes; -10.9 -10.3 nm on specimen axes Total_Shift = 7.697823 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.53 microns changed by -2.53 to target drift = 0.08 nm/sec Measured defocus = -0.95 microns changed by -0.05 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5805.tif 27 frames are being saved to U:\krios\20190717\PilA_5806.tif 27 frames are being saved to U:\krios\20190717\PilA_5807.tif 27 frames are being saved to U:\krios\20190717\PilA_5808.tif 27 frames are being saved to U:\krios\20190717\PilA_5809.tif 27 frames are being saved to U:\krios\20190717\PilA_5810.tif Running script 17 at item 95 (-715.02, 637.78) Remaining time to fill dewars is 30660 Dewars are NOT busy filling Trimming A by 22 167 20 210, reference by 180 22 20 185 Trimming A by 22 167 20 210, reference by 162 23 170 20 Trimming A by 22 167 20 210, reference by 24 166 20 200 Trimming A by 22 167 20 210, reference by 20 170 145 20 Disparity in stage position after aligning to center of map frame: 0.21 -0.17 Trimming A by 21 160 20 155, reference by 24 166 20 200 Disparity in stage position after aligning to target position: 0.30 -0.03 (2nd move error 0.08 0.12) Finished aligning to item in 2 rounds Trimming A by 22 155 20 155, reference by 21 160 20 155 0.6 -0.5 unbinned pixels; 2.7 1.3 nm along two shift axes; 1.9 2.4 nm on specimen axes Total_Shift = 1.589294 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.46 microns changed by -2.56 to target drift = 0.17 nm/sec Measured defocus = -1.10 microns changed by -0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5811.tif 27 frames are being saved to U:\krios\20190717\PilA_5812.tif 27 frames are being saved to U:\krios\20190717\PilA_5813.tif 27 frames are being saved to U:\krios\20190717\PilA_5814.tif 27 frames are being saved to U:\krios\20190717\PilA_5815.tif 27 frames are being saved to U:\krios\20190717\PilA_5816.tif Running script 17 at item 96 (-527.50, 654.93) Remaining time to fill dewars is 30540 Dewars are NOT busy filling Trimming A by 22 145 135 20, reference by 165 23 20 165 Trimming A by 22 145 135 20, reference by 155 22 140 20 Trimming A by 22 145 135 20, reference by 24 155 20 160 Trimming A by 22 145 135 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.19 Trimming A by 31 70 120 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.09 -0.22 (2nd move error 0.07 -0.05) Finished aligning to item in 2 rounds Trimming A by 22 62 130 20, reference by 31 70 120 20 -5.8 -0.5 unbinned pixels; 5.7 21.7 nm along two shift axes; 3.2 -22.5 nm on specimen axes Total_Shift = 11.65263 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.04 microns changed by -2.24 to target drift = 0.21 nm/sec Measured defocus = -1.19 microns changed by -0.01 to target drift = 0.15 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5817.tif 27 frames are being saved to U:\krios\20190717\PilA_5818.tif 27 frames are being saved to U:\krios\20190717\PilA_5819.tif 27 frames are being saved to U:\krios\20190717\PilA_5820.tif 27 frames are being saved to U:\krios\20190717\PilA_5821.tif 27 frames are being saved to U:\krios\20190717\PilA_5822.tif Running script 17 at item 97 (-529.10, 657.64) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Trimming A by 165 20 145 20, reference by 165 23 20 165 Trimming A by 165 20 145 20, reference by 155 22 140 20 Trimming A by 165 20 145 20, reference by 24 155 20 160 Trimming A by 165 20 145 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.18 Trimming A by 67 24 115 20, reference by 155 22 140 20 Disparity in stage position after aligning to target position: 0.07 -0.15 (2nd move error 0.01 0.01) Finished aligning to item in 2 rounds Trimming A by 67 24 115 20, reference by 67 24 115 20 2.1 -0.9 unbinned pixels; 5.8 6.1 nm along two shift axes; 2.9 8.2 nm on specimen axes Total_Shift = 4.445985 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.88 microns changed by -2.18 to target drift = 0.11 nm/sec Measured defocus = -1.23 microns changed by -0.07 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5823.tif 27 frames are being saved to U:\krios\20190717\PilA_5824.tif 27 frames are being saved to U:\krios\20190717\PilA_5825.tif 27 frames are being saved to U:\krios\20190717\PilA_5826.tif 27 frames are being saved to U:\krios\20190717\PilA_5827.tif 27 frames are being saved to U:\krios\20190717\PilA_5828.tif Running script 17 at item 98 (-526.10, 652.15) Remaining time to fill dewars is 30300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 140 175 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.03 -0.14 (2nd move error 0.02 0.03) Finished aligning to item in 1 rounds Trimming A by 24 140 175 20, reference by 24 140 175 20 0.2 -0.3 unbinned pixels; 1.4 0.1 nm along two shift axes; 1.2 0.7 nm on specimen axes Total_Shift = 0.70059 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.76 microns changed by -2.16 to target drift = 0.17 nm/sec Measured defocus = -1.34 microns changed by -0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5829.tif 27 frames are being saved to U:\krios\20190717\PilA_5830.tif 27 frames are being saved to U:\krios\20190717\PilA_5831.tif 27 frames are being saved to U:\krios\20190717\PilA_5832.tif 27 frames are being saved to U:\krios\20190717\PilA_5833.tif 27 frames are being saved to U:\krios\20190717\PilA_5834.tif Running script 17 at item 99 (-530.49, 660.33) Remaining time to fill dewars is 30120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 120 20 115 20, reference by 155 22 140 20 Disparity in stage position after aligning to target position: 0.09 -0.18 (2nd move error 0.03 -0.02) Finished aligning to item in 1 rounds Trimming A by 120 20 115 20, reference by 120 20 115 20 -4.4 -1.0 unbinned pixels; 2.2 17.5 nm along two shift axes; 4.7 -17.1 nm on specimen axes Total_Shift = 9.109047 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.80 microns changed by -2.30 to target drift = 0.10 nm/sec Measured defocus = -1.56 microns changed by 0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5835.tif 27 frames are being saved to U:\krios\20190717\PilA_5836.tif 27 frames are being saved to U:\krios\20190717\PilA_5837.tif 27 frames are being saved to U:\krios\20190717\PilA_5838.tif 27 frames are being saved to U:\krios\20190717\PilA_5839.tif 27 frames are being saved to U:\krios\20190717\PilA_5840.tif Running script 17 at item 100 (-531.91, 663.03) Remaining time to fill dewars is 30000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 155 20 130 20, reference by 155 22 140 20 Disparity in stage position after aligning to target position: 0.05 -0.18 (2nd move error -0.02 -0.02) Finished aligning to item in 1 rounds Trimming A by 155 20 130 20, reference by 155 20 130 20 -1.9 15.6 unbinned pixels; 59.9 16.5 nm along two shift axes; -60.3 -10.8 nm on specimen axes Total_Shift = 31.448403 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.65 microns changed by -2.25 to target drift = 0.08 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target drift = 0.00 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5841.tif 27 frames are being saved to U:\krios\20190717\PilA_5842.tif 27 frames are being saved to U:\krios\20190717\PilA_5843.tif 27 frames are being saved to U:\krios\20190717\PilA_5844.tif 27 frames are being saved to U:\krios\20190717\PilA_5845.tif 27 frames are being saved to U:\krios\20190717\PilA_5846.tif Running script 17 at item 101 (-535.96, 664.39) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 20 75 60, reference by 155 22 140 20 Disparity in stage position after aligning to target position: -0.04 -0.19 (2nd move error -0.10 -0.03) Finished aligning to item in 1 rounds Trimming A by 140 20 75 60, reference by 140 20 75 60 -3.2 20.1 unbinned pixels; 78.2 18.8 nm along two shift axes; -77.6 -16.7 nm on specimen axes Total_Shift = 40.771943 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.59 microns changed by -2.29 to target drift = 0.06 nm/sec Measured defocus = -1.72 microns changed by 0.02 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5847.tif 27 frames are being saved to U:\krios\20190717\PilA_5848.tif 27 frames are being saved to U:\krios\20190717\PilA_5849.tif 27 frames are being saved to U:\krios\20190717\PilA_5850.tif 27 frames are being saved to U:\krios\20190717\PilA_5851.tif 27 frames are being saved to U:\krios\20190717\PilA_5852.tif Running script 17 at item 102 (-534.52, 661.60) Remaining time to fill dewars is 29820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 22 65 20, reference by 155 22 140 20 Disparity in stage position after aligning to target position: 0.09 -0.21 (2nd move error 0.03 -0.04) Finished aligning to item in 1 rounds Trimming A by 90 22 65 20, reference by 90 22 65 20 -7.3 -1.2 unbinned pixels; 5.3 28.1 nm along two shift axes; 6.1 -28.2 nm on specimen axes Total_Shift = 14.817437 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.49 microns changed by -2.29 to target drift = 0.11 nm/sec Measured defocus = -1.82 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5853.tif 27 frames are being saved to U:\krios\20190717\PilA_5854.tif 27 frames are being saved to U:\krios\20190717\PilA_5855.tif 27 frames are being saved to U:\krios\20190717\PilA_5856.tif 27 frames are being saved to U:\krios\20190717\PilA_5857.tif 27 frames are being saved to U:\krios\20190717\PilA_5858.tif Running script 17 at item 103 (-533.10, 658.91) Remaining time to fill dewars is 29580 Dewars are NOT busy filling Trimming A by 160 22 145 20, reference by 165 23 20 165 Trimming A by 160 22 145 20, reference by 155 22 140 20 Trimming A by 160 22 145 20, reference by 24 155 20 160 Trimming A by 160 22 145 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.12 Trimming A by 60 24 50 20, reference by 155 22 140 20 Disparity in stage position after aligning to target position: 0.09 -0.23 (2nd move error -0.07 -0.14) Finished aligning to item in 2 rounds Trimming A by 55 20 50 20, reference by 60 24 50 20 -15.5 15.2 unbinned pixels; 76.2 32.7 nm along two shift axes; -56.0 -63.4 nm on specimen axes Total_Shift = 43.403211 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.38 microns changed by -2.28 to target drift = 0.03 nm/sec Measured defocus = -1.90 microns changed by 0.00 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5859.tif 27 frames are being saved to U:\krios\20190717\PilA_5860.tif 27 frames are being saved to U:\krios\20190717\PilA_5861.tif 27 frames are being saved to U:\krios\20190717\PilA_5862.tif 27 frames are being saved to U:\krios\20190717\PilA_5863.tif 27 frames are being saved to U:\krios\20190717\PilA_5864.tif Running script 17 at item 104 (-531.68, 656.31) Remaining time to fill dewars is 29520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 30 20 30 20, reference by 155 22 140 20 Disparity in stage position after aligning to target position: 0.23 -0.20 (2nd move error 0.07 -0.10) Finished aligning to item in 1 rounds Trimming A by 30 20 30 20, reference by 30 20 30 20 -12.5 -1.3 unbinned pixels; 11.3 46.9 nm along two shift axes; 7.9 -48.2 nm on specimen axes Total_Shift = 25.090881 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.31 microns changed by -2.31 to target drift = 0.16 nm/sec Measured defocus = -2.04 microns changed by 0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5865.tif 27 frames are being saved to U:\krios\20190717\PilA_5866.tif 27 frames are being saved to U:\krios\20190717\PilA_5867.tif 27 frames are being saved to U:\krios\20190717\PilA_5868.tif 27 frames are being saved to U:\krios\20190717\PilA_5869.tif 27 frames are being saved to U:\krios\20190717\PilA_5870.tif Running script 17 at item 105 (-530.18, 653.51) Remaining time to fill dewars is 29400 Dewars are NOT busy filling Trimming A by 22 145 130 20, reference by 165 23 20 165 Trimming A by 22 145 130 20, reference by 155 22 140 20 Trimming A by 22 145 130 20, reference by 24 155 20 160 Trimming A by 22 145 130 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.19 Trimming A by 24 70 70 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.14 -0.25 (2nd move error -0.01 -0.08) Finished aligning to item in 2 rounds Trimming A by 24 70 70 20, reference by 24 70 70 20 -10.5 2.8 unbinned pixels; 23.8 33.6 nm along two shift axes; -8.5 -41.4 nm on specimen axes Total_Shift = 21.678834 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.11 microns changed by -2.21 to target drift = 0.09 nm/sec Measured defocus = -2.06 microns changed by -0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5871.tif 27 frames are being saved to U:\krios\20190717\PilA_5872.tif 27 frames are being saved to U:\krios\20190717\PilA_5873.tif 27 frames are being saved to U:\krios\20190717\PilA_5874.tif 27 frames are being saved to U:\krios\20190717\PilA_5875.tif 27 frames are being saved to U:\krios\20190717\PilA_5876.tif Running script 17 at item 106 (-528.71, 650.72) Remaining time to fill dewars is 29160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 130 135 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.18 -0.23 (2nd move error 0.03 -0.06) Finished aligning to item in 1 rounds Trimming A by 23 135 135 20, reference by 20 130 135 20 -8.3 -1.4 unbinned pixels; 5.9 32.1 nm along two shift axes; 7.0 -32.2 nm on specimen axes Total_Shift = 16.897099 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.06 microns changed by -2.26 to target drift = 0.11 nm/sec Measured defocus = -2.19 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5877.tif 27 frames are being saved to U:\krios\20190717\PilA_5878.tif 27 frames are being saved to U:\krios\20190717\PilA_5879.tif 27 frames are being saved to U:\krios\20190717\PilA_5880.tif 27 frames are being saved to U:\krios\20190717\PilA_5881.tif 27 frames are being saved to U:\krios\20190717\PilA_5882.tif Running script 17 at item 107 (-531.37, 649.24) Remaining time to fill dewars is 29100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 110 105 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.26 -0.25 (2nd move error 0.11 -0.08) Finished aligning to item in 1 rounds Trimming A by 24 115 105 20, reference by 21 110 105 20 -7.8 -2.0 unbinned pixels; 2.8 31.3 nm along two shift axes; 9.5 -30.1 nm on specimen axes Total_Shift = 16.196222 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.05 microns changed by -2.35 to target drift = 0.13 nm/sec Measured defocus = -2.35 microns changed by 0.05 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5883.tif 27 frames are being saved to U:\krios\20190717\PilA_5884.tif 27 frames are being saved to U:\krios\20190717\PilA_5885.tif 27 frames are being saved to U:\krios\20190717\PilA_5886.tif 27 frames are being saved to U:\krios\20190717\PilA_5887.tif 27 frames are being saved to U:\krios\20190717\PilA_5888.tif Running script 17 at item 108 (-532.83, 652.04) Remaining time to fill dewars is 28980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 45 45 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.09 -0.32 (2nd move error -0.05 -0.15) Finished aligning to item in 1 rounds Trimming A by 20 45 45 20, reference by 20 45 45 20 -20.1 10.7 unbinned pixels; 65.6 56.3 nm along two shift axes; -37.4 -80.5 nm on specimen axes Total_Shift = 45.549218 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.03 microns changed by -2.37 to target drift = 0.02 nm/sec Measured defocus = -2.48 microns changed by 0.08 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5889.tif 27 frames are being saved to U:\krios\20190717\PilA_5890.tif 27 frames are being saved to U:\krios\20190717\PilA_5891.tif 27 frames are being saved to U:\krios\20190717\PilA_5892.tif 27 frames are being saved to U:\krios\20190717\PilA_5893.tif 27 frames are being saved to U:\krios\20190717\PilA_5894.tif Running script 17 at item 109 (-535.68, 657.50) Remaining time to fill dewars is 28800 Dewars are NOT busy filling Trimming A by 160 22 145 20, reference by 165 23 20 165 Trimming A by 160 22 145 20, reference by 155 22 140 20 Trimming A by 160 22 145 20, reference by 24 155 20 160 Trimming A by 160 22 145 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.18 -0.13 Trimming A by 20 20 20 20, reference by 155 22 140 20 Disparity in stage position after aligning to target position: 0.12 -0.23 (2nd move error -0.06 -0.12) Finished aligning to item in 2 rounds -14.9 13.2 unbinned pixels; 68.1 33.8 nm along two shift axes; -48.3 -60.8 nm on specimen axes Total_Shift = 39.856376 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.17 microns changed by -2.33 to target drift = 0.05 nm/sec Measured defocus = -2.51 microns changed by 0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5895.tif 27 frames are being saved to U:\krios\20190717\PilA_5896.tif 27 frames are being saved to U:\krios\20190717\PilA_5897.tif 27 frames are being saved to U:\krios\20190717\PilA_5898.tif 27 frames are being saved to U:\krios\20190717\PilA_5899.tif 27 frames are being saved to U:\krios\20190717\PilA_5900.tif Running script 17 at item 110 (-537.33, 660.24) Remaining time to fill dewars is 28680 Dewars are NOT busy filling Trimming A by 170 24 20 175, reference by 165 23 20 165 Trimming A by 170 24 20 175, reference by 155 22 140 20 Trimming A by 170 24 20 175, reference by 24 155 20 160 Trimming A by 170 24 20 175, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.09 Trimming A by 55 20 20 55, reference by 165 23 20 165 Disparity in stage position after aligning to target position: 0.32 -0.05 (2nd move error 0.19 0.03) Finished aligning to item in 2 rounds Trimming A by 50 21 20 55, reference by 55 20 20 55 -0.4 -1.6 unbinned pixels; 5.5 3.7 nm along two shift axes; 6.4 -1.0 nm on specimen axes Total_Shift = 3.342918 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.28 microns changed by -2.32 to target drift = 0.16 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5901.tif 27 frames are being saved to U:\krios\20190717\PilA_5902.tif 27 frames are being saved to U:\krios\20190717\PilA_5903.tif 27 frames are being saved to U:\krios\20190717\PilA_5904.tif 27 frames are being saved to U:\krios\20190717\PilA_5905.tif 27 frames are being saved to U:\krios\20190717\PilA_5906.tif Running script 17 at item 111 (-538.76, 662.74) Remaining time to fill dewars is 28440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 125 22 20 110, reference by 165 23 20 165 Disparity in stage position after aligning to target position: 0.34 -0.06 (2nd move error 0.21 0.01) Finished aligning to item in 1 rounds Trimming A by 125 21 20 105, reference by 125 22 20 110 1.1 -4.6 unbinned pixels; 18.5 3.0 nm along two shift axes; 17.8 5.3 nm on specimen axes Total_Shift = 9.521508 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.48 microns changed by -2.38 to target drift = 0.14 nm/sec Measured defocus = -0.84 microns changed by -0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5907.tif 27 frames are being saved to U:\krios\20190717\PilA_5908.tif 27 frames are being saved to U:\krios\20190717\PilA_5909.tif 27 frames are being saved to U:\krios\20190717\PilA_5910.tif 27 frames are being saved to U:\krios\20190717\PilA_5911.tif 27 frames are being saved to U:\krios\20190717\PilA_5912.tif Running script 17 at item 112 (-541.40, 661.29) Remaining time to fill dewars is 28380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 23 20 135, reference by 165 23 20 165 Disparity in stage position after aligning to target position: 0.25 -0.12 (2nd move error 0.12 -0.05) Finished aligning to item in 1 rounds Trimming A by 135 20 20 135, reference by 140 23 20 135 -6.6 -4.5 unbinned pixels; 8.1 30.7 nm along two shift axes; 19.1 -24.8 nm on specimen axes Total_Shift = 16.069872 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.51 microns changed by -2.51 to target drift = 0.11 nm/sec Measured defocus = -1.00 microns changed by -0.00 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5913.tif 27 frames are being saved to U:\krios\20190717\PilA_5914.tif 27 frames are being saved to U:\krios\20190717\PilA_5915.tif 27 frames are being saved to U:\krios\20190717\PilA_5916.tif 27 frames are being saved to U:\krios\20190717\PilA_5917.tif 27 frames are being saved to U:\krios\20190717\PilA_5918.tif Running script 17 at item 113 (-539.98, 658.66) Remaining time to fill dewars is 28260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 20 20 75, reference by 165 23 20 165 Disparity in stage position after aligning to target position: 0.35 -0.12 (2nd move error 0.22 -0.05) Finished aligning to item in 1 rounds Trimming A by 65 20 20 75, reference by 70 20 20 75 -4.3 -2.2 unbinned pixels; 2.4 19.0 nm along two shift axes; 9.5 -16.5 nm on specimen axes Total_Shift = 9.745915 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.40 microns changed by -2.50 to target drift = 0.10 nm/sec Measured defocus = -1.12 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5919.tif 27 frames are being saved to U:\krios\20190717\PilA_5920.tif 27 frames are being saved to U:\krios\20190717\PilA_5921.tif 27 frames are being saved to U:\krios\20190717\PilA_5922.tif 27 frames are being saved to U:\krios\20190717\PilA_5923.tif 27 frames are being saved to U:\krios\20190717\PilA_5924.tif Running script 17 at item 114 (-538.58, 655.93) Remaining time to fill dewars is 28020 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 165 23 20 165 Disparity in stage position after aligning to target position: 0.49 -0.08 (2nd move error 0.36 -0.01) Finished aligning to item in 1 rounds -0.4 1.5 unbinned pixels; 6.0 0.6 nm along two shift axes; -5.7 -2.1 nm on specimen axes Total_Shift = 3.093867 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.23 microns changed by -2.43 to target drift = 0.14 nm/sec Measured defocus = -1.21 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5925.tif 27 frames are being saved to U:\krios\20190717\PilA_5926.tif 27 frames are being saved to U:\krios\20190717\PilA_5927.tif 27 frames are being saved to U:\krios\20190717\PilA_5928.tif 27 frames are being saved to U:\krios\20190717\PilA_5929.tif 27 frames are being saved to U:\krios\20190717\PilA_5930.tif Running script 17 at item 115 (-536.96, 653.28) Remaining time to fill dewars is 27960 Dewars are NOT busy filling Trimming A by 20 142 20 180, reference by 165 23 20 165 Trimming A by 20 142 20 180, reference by 155 22 140 20 Trimming A by 20 142 20 180, reference by 24 155 20 160 Trimming A by 20 142 20 180, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.19 -0.15 Trimming A by 20 20 20 20, reference by 24 155 20 160 Disparity in stage position after aligning to target position: 0.22 0.02 (2nd move error 0.02 0.16) Finished aligning to item in 2 rounds -0.4 -0.1 unbinned pixels; 0.2 1.7 nm along two shift axes; 0.5 -1.6 nm on specimen axes Total_Shift = 0.859975 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.15 microns changed by -2.45 to target drift = 0.16 nm/sec Measured defocus = -1.26 microns changed by -0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5931.tif 27 frames are being saved to U:\krios\20190717\PilA_5932.tif 27 frames are being saved to U:\krios\20190717\PilA_5933.tif 27 frames are being saved to U:\krios\20190717\PilA_5934.tif 27 frames are being saved to U:\krios\20190717\PilA_5935.tif 27 frames are being saved to U:\krios\20190717\PilA_5936.tif Running script 17 at item 116 (-535.43, 650.56) Remaining time to fill dewars is 27840 Dewars are NOT busy filling Trimming A by 22 145 130 20, reference by 165 23 20 165 Trimming A by 22 145 130 20, reference by 155 22 140 20 Trimming A by 22 145 130 20, reference by 24 155 20 160 Trimming A by 22 145 130 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.13 Trimming A by 20 26 20 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.13 -0.36 (2nd move error -0.00 -0.24) Finished aligning to item in 2 rounds Trimming A by 20 26 20 20, reference by 20 26 20 20 -30.7 13.7 unbinned pixels; 90.3 90.0 nm along two shift axes; -46.6 -122.3 nm on specimen axes Total_Shift = 67.20488 Trimming A by 20 30 20 20, reference by 20 26 20 20 -1.8 10.5 unbinned pixels; 40.8 9.2 nm along two shift axes; -40.3 -9.3 nm on specimen axes Total_Shift = 21.242741 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.06 microns changed by -2.46 to target drift = 0.01 nm/sec Measured defocus = -1.40 microns changed by 0.00 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5937.tif 27 frames are being saved to U:\krios\20190717\PilA_5938.tif 27 frames are being saved to U:\krios\20190717\PilA_5939.tif 27 frames are being saved to U:\krios\20190717\PilA_5940.tif 27 frames are being saved to U:\krios\20190717\PilA_5941.tif 27 frames are being saved to U:\krios\20190717\PilA_5942.tif Running script 17 at item 117 (-534.02, 647.86) Remaining time to fill dewars is 27660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 95 70 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.19 -0.27 (2nd move error 0.05 -0.16) Finished aligning to item in 1 rounds Trimming A by 21 100 70 20, reference by 22 95 70 20 -20.7 0.4 unbinned pixels; 28.5 73.8 nm along two shift axes; 2.8 -80.5 nm on specimen axes Total_Shift = 41.356611 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.95 microns changed by -2.45 to target drift = 0.12 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5943.tif 27 frames are being saved to U:\krios\20190717\PilA_5944.tif 27 frames are being saved to U:\krios\20190717\PilA_5945.tif 27 frames are being saved to U:\krios\20190717\PilA_5946.tif 27 frames are being saved to U:\krios\20190717\PilA_5947.tif 27 frames are being saved to U:\krios\20190717\PilA_5948.tif Running script 17 at item 118 (-532.60, 645.16) Remaining time to fill dewars is 27540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 175 120 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.20 -0.21 (2nd move error 0.06 -0.09) Finished aligning to item in 1 rounds Trimming A by 24 180 120 20, reference by 22 175 120 20 -11.0 -1.0 unbinned pixels; 10.6 41.0 nm along two shift axes; 6.2 -42.4 nm on specimen axes Total_Shift = 22.01459 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.83 microns changed by -2.43 to target drift = 0.11 nm/sec Measured defocus = -1.64 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5949.tif 27 frames are being saved to U:\krios\20190717\PilA_5950.tif 27 frames are being saved to U:\krios\20190717\PilA_5951.tif 27 frames are being saved to U:\krios\20190717\PilA_5952.tif 27 frames are being saved to U:\krios\20190717\PilA_5953.tif 27 frames are being saved to U:\krios\20190717\PilA_5954.tif Running script 17 at item 119 (-536.82, 646.37) Remaining time to fill dewars is 27300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 130 20 100, reference by 24 155 20 160 Disparity in stage position after aligning to target position: 0.29 -0.10 (2nd move error 0.09 0.04) Finished aligning to item in 1 rounds Trimming A by 21 130 70 95, reference by 20 130 20 100 0.5 -1.3 unbinned pixels; 5.2 0.3 nm along two shift axes; 4.8 2.0 nm on specimen axes Total_Shift = 2.676474 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.80 microns changed by -2.50 to target drift = 0.13 nm/sec Measured defocus = -1.71 microns changed by 0.01 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5955.tif 27 frames are being saved to U:\krios\20190717\PilA_5956.tif 27 frames are being saved to U:\krios\20190717\PilA_5957.tif 27 frames are being saved to U:\krios\20190717\PilA_5958.tif 27 frames are being saved to U:\krios\20190717\PilA_5959.tif 27 frames are being saved to U:\krios\20190717\PilA_5960.tif Running script 17 at item 120 (-538.19, 649.00) Remaining time to fill dewars is 27120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 80 20 95, reference by 24 155 20 160 Disparity in stage position after aligning to target position: 0.33 -0.09 (2nd move error 0.13 0.05) Finished aligning to item in 1 rounds Trimming A by 20 80 20 90, reference by 22 80 20 95 0.4 1.3 unbinned pixels; 4.1 3.4 nm along two shift axes; -5.0 1.3 nm on specimen axes Total_Shift = 2.644885 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.64 microns changed by -2.44 to target drift = 0.14 nm/sec Measured defocus = -1.73 microns changed by -0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5961.tif 27 frames are being saved to U:\krios\20190717\PilA_5962.tif 27 frames are being saved to U:\krios\20190717\PilA_5963.tif 27 frames are being saved to U:\krios\20190717\PilA_5964.tif 27 frames are being saved to U:\krios\20190717\PilA_5965.tif 27 frames are being saved to U:\krios\20190717\PilA_5966.tif Running script 17 at item 121 (-539.58, 651.67) Remaining time to fill dewars is 27000 Dewars are NOT busy filling Trimming A by 20 142 20 180, reference by 165 23 20 165 Trimming A by 20 142 20 180, reference by 155 22 140 20 Trimming A by 20 142 20 180, reference by 24 155 20 160 Trimming A by 20 142 20 180, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.18 -0.15 Trimming A by 24 51 20 80, reference by 24 155 20 160 Disparity in stage position after aligning to target position: 0.25 -0.08 (2nd move error 0.07 0.05) Finished aligning to item in 2 rounds Trimming A by 22 42 20 85, reference by 24 51 20 80 -1.9 -2.8 unbinned pixels; 7.9 11.3 nm along two shift axes; 11.4 -7.0 nm on specimen axes Total_Shift = 6.874048 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.51 microns changed by -2.41 to target drift = 0.15 nm/sec Measured defocus = -1.83 microns changed by -0.07 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5967.tif 27 frames are being saved to U:\krios\20190717\PilA_5968.tif 27 frames are being saved to U:\krios\20190717\PilA_5969.tif 27 frames are being saved to U:\krios\20190717\PilA_5970.tif 27 frames are being saved to U:\krios\20190717\PilA_5971.tif 27 frames are being saved to U:\krios\20190717\PilA_5972.tif Running script 17 at item 122 (-540.93, 654.47) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 60, reference by 24 155 20 160 Disparity in stage position after aligning to target position: 0.16 -0.09 (2nd move error -0.02 0.05) Finished aligning to item in 1 rounds Trimming A by 20 30 20 50, reference by 20 20 20 60 -1.2 4.5 unbinned pixels; 18.1 2.5 nm along two shift axes; -17.3 -5.5 nm on specimen axes Total_Shift = 9.315255 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.46 microns changed by -2.46 to target drift = 0.10 nm/sec Measured defocus = -2.00 microns changed by 0.00 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5973.tif 27 frames are being saved to U:\krios\20190717\PilA_5974.tif 27 frames are being saved to U:\krios\20190717\PilA_5975.tif 27 frames are being saved to U:\krios\20190717\PilA_5976.tif 27 frames are being saved to U:\krios\20190717\PilA_5977.tif 27 frames are being saved to U:\krios\20190717\PilA_5978.tif Running script 17 at item 123 (-542.58, 657.26) Remaining time to fill dewars is 26700 Dewars are NOT busy filling Trimming A by 175 24 20 175, reference by 165 23 20 165 Trimming A by 175 24 20 175, reference by 155 22 140 20 Trimming A by 175 24 20 175, reference by 24 155 20 160 Trimming A by 175 24 20 175, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.22 -0.12 Trimming A by 72 23 20 120, reference by 165 23 20 165 Disparity in stage position after aligning to target position: 0.38 -0.15 (2nd move error 0.15 -0.05) Finished aligning to item in 2 rounds Trimming A by 85 20 20 100, reference by 72 23 20 120 -5.2 1.1 unbinned pixels; 10.9 17.0 nm along two shift axes; -3.2 -20.5 nm on specimen axes Total_Shift = 10.634586 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.33 microns changed by -2.43 to target drift = 0.13 nm/sec Measured defocus = -2.04 microns changed by -0.06 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5979.tif 27 frames are being saved to U:\krios\20190717\PilA_5980.tif 27 frames are being saved to U:\krios\20190717\PilA_5981.tif 27 frames are being saved to U:\krios\20190717\PilA_5982.tif 27 frames are being saved to U:\krios\20190717\PilA_5983.tif 27 frames are being saved to U:\krios\20190717\PilA_5984.tif Running script 17 at item 124 (-543.64, 652.99) Remaining time to fill dewars is 26580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 85 20 125, reference by 24 155 20 160 Disparity in stage position after aligning to target position: 0.13 -0.13 (2nd move error -0.06 0.00) Finished aligning to item in 1 rounds Trimming A by 21 85 20 125, reference by 21 85 20 125 -0.8 13.6 unbinned pixels; 51.0 17.6 nm along two shift axes; -52.7 -5.9 nm on specimen axes Total_Shift = 27.226805 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.35 microns changed by -2.55 to target drift = 0.10 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5985.tif 27 frames are being saved to U:\krios\20190717\PilA_5986.tif 27 frames are being saved to U:\krios\20190717\PilA_5987.tif 27 frames are being saved to U:\krios\20190717\PilA_5988.tif 27 frames are being saved to U:\krios\20190717\PilA_5989.tif 27 frames are being saved to U:\krios\20190717\PilA_5990.tif Running script 17 at item 125 (-542.14, 650.23) Remaining time to fill dewars is 26400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 115 20 140, reference by 24 155 20 160 Disparity in stage position after aligning to target position: 0.25 -0.12 (2nd move error 0.07 0.02) Finished aligning to item in 1 rounds Trimming A by 23 120 20 135, reference by 20 115 20 140 1.6 -1.7 unbinned pixels; 8.3 3.3 nm along two shift axes; 6.2 6.7 nm on specimen axes Total_Shift = 4.663986 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.21 microns changed by -2.51 to target drift = 0.12 nm/sec Measured defocus = -2.28 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5991.tif 27 frames are being saved to U:\krios\20190717\PilA_5992.tif 27 frames are being saved to U:\krios\20190717\PilA_5993.tif 27 frames are being saved to U:\krios\20190717\PilA_5994.tif 27 frames are being saved to U:\krios\20190717\PilA_5995.tif 27 frames are being saved to U:\krios\20190717\PilA_5996.tif Running script 17 at item 126 (-540.78, 647.54) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 142 20 165, reference by 24 155 20 160 Disparity in stage position after aligning to target position: 0.35 -0.09 (2nd move error 0.16 0.04) Finished aligning to item in 1 rounds Trimming A by 20 150 20 150, reference by 24 142 20 165 0.3 3.2 unbinned pixels; 11.4 5.9 nm along two shift axes; -12.5 0.5 nm on specimen axes Total_Shift = 6.445403 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.11 microns changed by -2.51 to target drift = 0.14 nm/sec Measured defocus = -2.36 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_5997.tif 27 frames are being saved to U:\krios\20190717\PilA_5998.tif 27 frames are being saved to U:\krios\20190717\PilA_5999.tif 27 frames are being saved to U:\krios\20190717\PilA_6000.tif 27 frames are being saved to U:\krios\20190717\PilA_6001.tif 27 frames are being saved to U:\krios\20190717\PilA_6002.tif Running script 17 at item 127 (-568.03, 604.20) Remaining time to fill dewars is 26160 Dewars are NOT busy filling Trimming A by 23 130 105 20, reference by 210 22 20 230 Trimming A by 23 130 105 20, reference by 135 24 110 20 Trimming A by 23 130 105 20, reference by 20 182 20 240 Trimming A by 23 130 105 20, reference by 23 130 105 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.16 Trimming A by 24 131 180 20, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.08 -0.05 (2nd move error 0.00 0.10) Finished aligning to item in 2 rounds Trimming A by 23 140 170 20, reference by 24 131 180 20 0.0 1.2 unbinned pixels; 4.5 1.9 nm along two shift axes; -4.7 -0.2 nm on specimen axes Total_Shift = 2.432939 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -1.51 microns changed by -0.99 to target drift = 0.26 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6003.tif 27 frames are being saved to U:\krios\20190717\PilA_6004.tif 27 frames are being saved to U:\krios\20190717\PilA_6005.tif 27 frames are being saved to U:\krios\20190717\PilA_6006.tif 27 frames are being saved to U:\krios\20190717\PilA_6007.tif 27 frames are being saved to U:\krios\20190717\PilA_6008.tif Running script 17 at item 128 (-569.45, 606.90) Remaining time to fill dewars is 25980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 110 20, reference by 23 130 105 20 Disparity in stage position after aligning to target position: -0.02 -0.13 (2nd move error -0.10 0.02) Finished aligning to item in 1 rounds Trimming A by 20 80 110 20, reference by 20 80 110 20 3.6 18.9 unbinned pixels; 64.9 41.6 nm along two shift axes; -74.4 10.1 nm on specimen axes Total_Shift = 38.568814 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.37 microns changed by -2.23 to target drift = 0.11 nm/sec Measured defocus = -2.60 microns changed by -0.00 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6009.tif 27 frames are being saved to U:\krios\20190717\PilA_6010.tif 27 frames are being saved to U:\krios\20190717\PilA_6011.tif 27 frames are being saved to U:\krios\20190717\PilA_6012.tif 27 frames are being saved to U:\krios\20190717\PilA_6013.tif 27 frames are being saved to U:\krios\20190717\PilA_6014.tif Running script 17 at item 129 (-571.21, 609.69) Remaining time to fill dewars is 25740 Dewars are NOT busy filling Trimming A by 145 22 110 20, reference by 210 22 20 230 Trimming A by 145 22 110 20, reference by 135 24 110 20 Trimming A by 145 22 110 20, reference by 20 182 20 240 Trimming A by 145 22 110 20, reference by 23 130 105 20 Disparity in stage position after aligning to center of map frame: 0.20 -0.12 Trimming A by 61 24 90 20, reference by 135 24 110 20 Disparity in stage position after aligning to target position: 0.32 -0.08 (2nd move error 0.11 0.02) Finished aligning to item in 2 rounds Trimming A by 61 20 95 20, reference by 61 24 90 20 0.2 -1.0 unbinned pixels; 4.1 0.9 nm along two shift axes; 4.0 1.0 nm on specimen axes Total_Shift = 2.114511 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.44 microns changed by -2.34 to target drift = 0.13 nm/sec Measured defocus = -0.85 microns changed by -0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6015.tif 27 frames are being saved to U:\krios\20190717\PilA_6016.tif 27 frames are being saved to U:\krios\20190717\PilA_6017.tif 27 frames are being saved to U:\krios\20190717\PilA_6018.tif 27 frames are being saved to U:\krios\20190717\PilA_6019.tif 27 frames are being saved to U:\krios\20190717\PilA_6020.tif Running script 17 at item 130 (-572.56, 612.35) Remaining time to fill dewars is 25680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 91 23 105 20, reference by 135 24 110 20 Disparity in stage position after aligning to target position: 0.31 -0.15 (2nd move error 0.11 -0.03) Finished aligning to item in 1 rounds Trimming A by 105 22 95 20, reference by 91 23 105 20 -4.2 -2.2 unbinned pixels; 2.6 18.3 nm along two shift axes; 9.4 -15.8 nm on specimen axes Total_Shift = 9.437283 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.32 microns changed by -2.32 to target drift = 0.09 nm/sec Measured defocus = -1.01 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6021.tif 27 frames are being saved to U:\krios\20190717\PilA_6022.tif 27 frames are being saved to U:\krios\20190717\PilA_6023.tif 27 frames are being saved to U:\krios\20190717\PilA_6024.tif 27 frames are being saved to U:\krios\20190717\PilA_6025.tif 27 frames are being saved to U:\krios\20190717\PilA_6026.tif Running script 17 at item 131 (-574.01, 615.08) Remaining time to fill dewars is 25560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 21 110 20, reference by 135 24 110 20 Disparity in stage position after aligning to target position: 0.27 -0.14 (2nd move error 0.06 -0.03) Finished aligning to item in 1 rounds Trimming A by 150 21 110 20, reference by 150 21 110 20 -3.7 -1.8 unbinned pixels; 1.6 16.1 nm along two shift axes; 7.7 -14.2 nm on specimen axes Total_Shift = 8.271259 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.21 microns changed by -2.31 to target drift = 0.12 nm/sec Measured defocus = -1.05 microns changed by -0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6027.tif 27 frames are being saved to U:\krios\20190717\PilA_6028.tif 27 frames are being saved to U:\krios\20190717\PilA_6029.tif 27 frames are being saved to U:\krios\20190717\PilA_6030.tif 27 frames are being saved to U:\krios\20190717\PilA_6031.tif 27 frames are being saved to U:\krios\20190717\PilA_6032.tif Running script 17 at item 132 (-578.06, 616.41) Remaining time to fill dewars is 25380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 165 22 25 105, reference by 135 24 110 20 Disparity in stage position after aligning to target position: 0.11 -0.19 (2nd move error -0.09 -0.08) Finished aligning to item in 1 rounds Trimming A by 165 21 20 105, reference by 165 22 25 105 -11.3 18.3 unbinned pixels; 82.1 13.2 nm along two shift axes; -68.9 -47.9 nm on specimen axes Total_Shift = 43.075259 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.29 microns changed by -2.49 to target drift = 0.08 nm/sec Measured defocus = -1.20 microns changed by 0.00 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6033.tif 27 frames are being saved to U:\krios\20190717\PilA_6034.tif 27 frames are being saved to U:\krios\20190717\PilA_6035.tif 27 frames are being saved to U:\krios\20190717\PilA_6036.tif 27 frames are being saved to U:\krios\20190717\PilA_6037.tif 27 frames are being saved to U:\krios\20190717\PilA_6038.tif Running script 17 at item 133 (-576.69, 613.65) Remaining time to fill dewars is 25320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 85 20 50 60, reference by 135 24 110 20 Disparity in stage position after aligning to target position: 0.32 -0.19 (2nd move error 0.11 -0.08) Finished aligning to item in 1 rounds Trimming A by 85 21 50 55, reference by 85 20 50 60 -6.6 0.3 unbinned pixels; 9.8 23.1 nm along two shift axes; 0.0 -25.6 nm on specimen axes Total_Shift = 13.139262 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.22 microns changed by -2.52 to target drift = 0.09 nm/sec Measured defocus = -1.35 microns changed by 0.05 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6039.tif 27 frames are being saved to U:\krios\20190717\PilA_6040.tif 27 frames are being saved to U:\krios\20190717\PilA_6041.tif 27 frames are being saved to U:\krios\20190717\PilA_6042.tif 27 frames are being saved to U:\krios\20190717\PilA_6043.tif 27 frames are being saved to U:\krios\20190717\PilA_6044.tif Running script 17 at item 134 (-575.16, 610.96) Remaining time to fill dewars is 25080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 45 20 35 20, reference by 135 24 110 20 Disparity in stage position after aligning to target position: 0.23 -0.18 (2nd move error 0.09 -0.06) Finished aligning to item in 1 rounds Trimming A by 40 20 35 20, reference by 45 20 35 20 -9.0 -16.4 unbinned pixels; 48.5 57.2 nm along two shift axes; 65.7 -31.7 nm on specimen axes Total_Shift = 37.436316 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.99 microns changed by -2.39 to target drift = 0.07 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6045.tif 27 frames are being saved to U:\krios\20190717\PilA_6046.tif 27 frames are being saved to U:\krios\20190717\PilA_6047.tif 27 frames are being saved to U:\krios\20190717\PilA_6048.tif 27 frames are being saved to U:\krios\20190717\PilA_6049.tif 27 frames are being saved to U:\krios\20190717\PilA_6050.tif Running script 17 at item 135 (-573.52, 608.12) Remaining time to fill dewars is 24900 Dewars are NOT busy filling Trimming A by 145 22 110 20, reference by 210 22 20 230 Trimming A by 145 22 110 20, reference by 135 24 110 20 Trimming A by 145 22 110 20, reference by 20 182 20 240 Trimming A by 145 22 110 20, reference by 23 130 105 20 Disparity in stage position after aligning to center of map frame: 0.32 -0.13 Trimming A by 20 20 20 20, reference by 135 24 110 20 Disparity in stage position after aligning to target position: 0.37 -0.12 (2nd move error 0.05 0.01) Finished aligning to item in 2 rounds 1.1 -8.4 unbinned pixels; 32.2 8.8 nm along two shift axes; 32.4 5.9 nm on specimen axes Total_Shift = 16.904613 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.85 microns changed by -2.35 to target drift = 0.15 nm/sec Measured defocus = -1.49 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6051.tif 27 frames are being saved to U:\krios\20190717\PilA_6052.tif 27 frames are being saved to U:\krios\20190717\PilA_6053.tif 27 frames are being saved to U:\krios\20190717\PilA_6054.tif 27 frames are being saved to U:\krios\20190717\PilA_6055.tif 27 frames are being saved to U:\krios\20190717\PilA_6056.tif Running script 17 at item 136 (-572.09, 605.43) Remaining time to fill dewars is 24780 Dewars are NOT busy filling Trimming A by 23 130 100 20, reference by 210 22 20 230 Trimming A by 23 130 100 20, reference by 135 24 110 20 Trimming A by 23 130 100 20, reference by 20 182 20 240 Trimming A by 23 130 100 20, reference by 23 130 105 20 Disparity in stage position after aligning to center of map frame: 0.25 -0.21 Trimming A by 23 52 85 20, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.21 -0.14 (2nd move error -0.05 0.05) Finished aligning to item in 2 rounds Trimming A by 22 60 75 20, reference by 23 52 85 20 3.8 10.8 unbinned pixels; 34.9 30.0 nm along two shift axes; -43.0 12.5 nm on specimen axes Total_Shift = 22.96672 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.73 microns changed by -2.33 to target drift = 0.15 nm/sec Measured defocus = -1.55 microns changed by -0.05 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6057.tif 27 frames are being saved to U:\krios\20190717\PilA_6058.tif 27 frames are being saved to U:\krios\20190717\PilA_6059.tif 27 frames are being saved to U:\krios\20190717\PilA_6060.tif 27 frames are being saved to U:\krios\20190717\PilA_6061.tif 27 frames are being saved to U:\krios\20190717\PilA_6062.tif Running script 17 at item 137 (-570.69, 602.68) Remaining time to fill dewars is 24720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 135 130 20, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.22 -0.13 (2nd move error -0.04 0.07) Finished aligning to item in 1 rounds Trimming A by 24 135 125 20, reference by 24 135 130 20 -1.4 7.7 unbinned pixels; 30.3 6.5 nm along two shift axes; -29.8 -7.2 nm on specimen axes Total_Shift = 15.736864 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.61 microns changed by -2.31 to target drift = 0.10 nm/sec Measured defocus = -1.68 microns changed by -0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6063.tif 27 frames are being saved to U:\krios\20190717\PilA_6064.tif 27 frames are being saved to U:\krios\20190717\PilA_6065.tif 27 frames are being saved to U:\krios\20190717\PilA_6066.tif 27 frames are being saved to U:\krios\20190717\PilA_6067.tif 27 frames are being saved to U:\krios\20190717\PilA_6068.tif Running script 17 at item 138 (-573.34, 601.31) Remaining time to fill dewars is 24360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 120 95 20, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.24 -0.23 (2nd move error -0.02 -0.03) Finished aligning to item in 1 rounds Trimming A by 24 120 95 20, reference by 24 120 95 20 -4.2 4.8 unbinned pixels; 23.0 8.1 nm along two shift axes; -17.6 -17.5 nm on specimen axes Total_Shift = 12.752624 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.67 microns changed by -2.47 to target drift = 0.02 nm/sec Measured defocus = -1.86 microns changed by 0.06 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6069.tif 27 frames are being saved to U:\krios\20190717\PilA_6070.tif 27 frames are being saved to U:\krios\20190717\PilA_6071.tif 27 frames are being saved to U:\krios\20190717\PilA_6072.tif 27 frames are being saved to U:\krios\20190717\PilA_6073.tif 27 frames are being saved to U:\krios\20190717\PilA_6074.tif Running script 17 at item 139 (-574.75, 604.03) Remaining time to fill dewars is 24300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 45 45 20, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.18 -0.25 (2nd move error -0.08 -0.06) Finished aligning to item in 1 rounds Trimming A by 20 50 40 20, reference by 20 45 45 20 -7.4 14.0 unbinned pixels; 61.2 5.5 nm along two shift axes; -53.0 -31.7 nm on specimen axes Total_Shift = 31.696502 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.54 microns changed by -2.44 to target drift = 0.06 nm/sec Measured defocus = -1.94 microns changed by 0.04 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6075.tif 27 frames are being saved to U:\krios\20190717\PilA_6076.tif 27 frames are being saved to U:\krios\20190717\PilA_6077.tif 27 frames are being saved to U:\krios\20190717\PilA_6078.tif 27 frames are being saved to U:\krios\20190717\PilA_6079.tif 27 frames are being saved to U:\krios\20190717\PilA_6080.tif Running script 17 at item 140 (-576.14, 606.87) Remaining time to fill dewars is 24120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.10 -0.22 (2nd move error -0.15 -0.02) Finished aligning to item in 1 rounds -1.2 27.8 unbinned pixels; 103.7 37.3 nm along two shift axes; -107.7 -10.7 nm on specimen axes Total_Shift = 55.582655 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.39 microns changed by -2.39 to target drift = 0.04 nm/sec Measured defocus = -1.94 microns changed by -0.06 to target drift = 0.00 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6081.tif 27 frames are being saved to U:\krios\20190717\PilA_6082.tif 27 frames are being saved to U:\krios\20190717\PilA_6083.tif 27 frames are being saved to U:\krios\20190717\PilA_6084.tif 27 frames are being saved to U:\krios\20190717\PilA_6085.tif 27 frames are being saved to U:\krios\20190717\PilA_6086.tif Running script 17 at item 141 (-577.85, 609.58) Remaining time to fill dewars is 23940 Dewars are NOT busy filling Trimming A by 145 21 105 20, reference by 210 22 20 230 Trimming A by 145 21 105 20, reference by 135 24 110 20 Trimming A by 145 21 105 20, reference by 20 182 20 240 Trimming A by 145 21 105 20, reference by 23 130 105 20 Disparity in stage position after aligning to center of map frame: 0.35 -0.13 Trimming A by 20 20 20 20, reference by 135 24 110 20 Disparity in stage position after aligning to target position: 0.28 -0.25 (2nd move error -0.09 -0.14) Finished aligning to item in 2 rounds -18.1 27.5 unbinned pixels; 124.6 23.7 nm along two shift axes; -103.1 -76.1 nm on specimen axes Total_Shift = 65.771745 -7.7 18.3 unbinned pixels; 77.6 0.3 nm along two shift axes; -69.7 -34.0 nm on specimen axes Total_Shift = 39.819988 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.39 microns changed by -2.49 to target drift = 0.01 nm/sec Measured defocus = -2.17 microns changed by 0.07 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6087.tif 27 frames are being saved to U:\krios\20190717\PilA_6088.tif 27 frames are being saved to U:\krios\20190717\PilA_6089.tif 27 frames are being saved to U:\krios\20190717\PilA_6090.tif 27 frames are being saved to U:\krios\20190717\PilA_6091.tif 27 frames are being saved to U:\krios\20190717\PilA_6092.tif Running script 17 at item 142 (-579.24, 612.28) Remaining time to fill dewars is 23760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 24 20 85, reference by 135 24 110 20 Disparity in stage position after aligning to target position: 0.28 -0.31 (2nd move error -0.06 -0.21) Finished aligning to item in 1 rounds Trimming A by 90 21 55 80, reference by 90 24 20 85 -25.9 13.3 unbinned pixels; 82.9 73.2 nm along two shift axes; -46.4 -103.6 nm on specimen axes Total_Shift = 58.290209 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.25 microns changed by -2.45 to target drift = 0.01 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6093.tif 27 frames are being saved to U:\krios\20190717\PilA_6094.tif 27 frames are being saved to U:\krios\20190717\PilA_6095.tif 27 frames are being saved to U:\krios\20190717\PilA_6096.tif 27 frames are being saved to U:\krios\20190717\PilA_6097.tif 27 frames are being saved to U:\krios\20190717\PilA_6098.tif Running script 17 at item 143 (-581.96, 610.67) Remaining time to fill dewars is 23460 Dewars are NOT busy filling Trimming A by 215 20 20 245, reference by 210 22 20 230 Trimming A by 215 20 20 245, reference by 135 24 110 20 Trimming A by 215 20 20 245, reference by 20 182 20 240 Trimming A by 215 20 20 245, reference by 23 130 105 20 Disparity in stage position after aligning to center of map frame: 0.21 -0.12 Trimming A by 87 22 20 130, reference by 210 22 20 230 Disparity in stage position after aligning to target position: 0.39 0.00 (2nd move error 0.18 0.11) Finished aligning to item in 2 rounds Trimming A by 100 24 20 115, reference by 87 22 20 130 -1.4 3.1 unbinned pixels; 13.2 0.3 nm along two shift axes; -11.8 -6.0 nm on specimen axes Total_Shift = 6.783275 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.27 microns changed by -2.57 to target drift = 0.15 nm/sec Measured defocus = -2.33 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6099.tif 27 frames are being saved to U:\krios\20190717\PilA_6100.tif 27 frames are being saved to U:\krios\20190717\PilA_6101.tif 27 frames are being saved to U:\krios\20190717\PilA_6102.tif 27 frames are being saved to U:\krios\20190717\PilA_6103.tif 27 frames are being saved to U:\krios\20190717\PilA_6104.tif Running script 17 at item 144 (-580.57, 608.04) Remaining time to fill dewars is 23340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 40 20 20 50, reference by 210 22 20 230 Disparity in stage position after aligning to target position: 0.45 -0.12 (2nd move error 0.24 -0.01) Finished aligning to item in 1 rounds Trimming A by 30 20 20 50, reference by 40 20 20 50 -1.7 -4.9 unbinned pixels; 16.0 13.4 nm along two shift axes; 19.6 -5.5 nm on specimen axes Total_Shift = 10.42603 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.09 microns changed by -2.49 to target drift = 0.16 nm/sec Measured defocus = -2.40 microns changed by -0.00 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6105.tif 27 frames are being saved to U:\krios\20190717\PilA_6106.tif 27 frames are being saved to U:\krios\20190717\PilA_6107.tif 27 frames are being saved to U:\krios\20190717\PilA_6108.tif 27 frames are being saved to U:\krios\20190717\PilA_6109.tif 27 frames are being saved to U:\krios\20190717\PilA_6110.tif Running script 17 at item 145 (-578.70, 605.36) Remaining time to fill dewars is 23160 Dewars are NOT busy filling Trimming A by 23 130 100 20, reference by 210 22 20 230 Trimming A by 23 130 100 20, reference by 135 24 110 20 Trimming A by 23 130 100 20, reference by 20 182 20 240 Trimming A by 23 130 100 20, reference by 23 130 105 20 Disparity in stage position after aligning to center of map frame: 0.23 -0.17 Trimming A by 20 30 20 50, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.09 -0.31 (2nd move error -0.15 -0.15) Finished aligning to item in 2 rounds Trimming A by 21 40 20 45, reference by 20 30 20 50 -15.1 26.8 unbinned pixels; 118.4 14.1 nm along two shift axes; -101.2 -64.6 nm on specimen axes Total_Shift = 61.599695 Trimming A by 21 40 20 50, reference by 20 30 20 50 -3.9 16.9 unbinned pixels; 67.2 11.6 nm along two shift axes; -65.0 -18.6 nm on specimen axes Total_Shift = 34.706395 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Using peak at 21.2,-215.0, rejecting 1.81 times higher peak at -0.0,-0.4 Measured defocus = -2.28 microns changed by -0.22 to target drift = 2.42 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6111.tif 27 frames are being saved to U:\krios\20190717\PilA_6112.tif 27 frames are being saved to U:\krios\20190717\PilA_6113.tif 27 frames are being saved to U:\krios\20190717\PilA_6114.tif 27 frames are being saved to U:\krios\20190717\PilA_6115.tif 27 frames are being saved to U:\krios\20190717\PilA_6116.tif Running script 17 at item 146 (-577.39, 602.57) Remaining time to fill dewars is 22920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 75 20 70, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.17 -0.29 (2nd move error -0.07 -0.13) Finished aligning to item in 1 rounds Trimming A by 21 75 45 70, reference by 24 75 20 70 -15.8 15.8 unbinned pixels; 78.9 33.2 nm along two shift axes; -58.2 -65.0 nm on specimen axes Total_Shift = 44.778931 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.14 microns changed by -2.46 to target drift = 0.04 nm/sec Measured defocus = -2.63 microns changed by 0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6117.tif 27 frames are being saved to U:\krios\20190717\PilA_6118.tif 27 frames are being saved to U:\krios\20190717\PilA_6119.tif 27 frames are being saved to U:\krios\20190717\PilA_6120.tif 27 frames are being saved to U:\krios\20190717\PilA_6121.tif 27 frames are being saved to U:\krios\20190717\PilA_6122.tif Running script 17 at item 147 (-575.98, 599.84) Remaining time to fill dewars is 22740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 120 50 75, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.23 -0.26 (2nd move error -0.01 -0.10) Finished aligning to item in 1 rounds Trimming A by 20 120 50 80, reference by 20 120 50 75 -13.3 5.1 unbinned pixels; 36.0 40.1 nm along two shift axes; -17.0 -52.7 nm on specimen axes Total_Shift = 28.416759 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 0.30 microns changed by -1.20 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6123.tif 27 frames are being saved to U:\krios\20190717\PilA_6124.tif 27 frames are being saved to U:\krios\20190717\PilA_6125.tif 27 frames are being saved to U:\krios\20190717\PilA_6126.tif 27 frames are being saved to U:\krios\20190717\PilA_6127.tif 27 frames are being saved to U:\krios\20190717\PilA_6128.tif Running script 17 at item 148 (-578.64, 598.53) Remaining time to fill dewars is 22560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 160 20 155, reference by 23 130 105 20 Disparity in stage position after aligning to target position: 0.24 -0.31 (2nd move error -0.00 -0.15) Finished aligning to item in 1 rounds Trimming A by 22 165 95 150, reference by 20 160 20 155 -18.4 3.3 unbinned pixels; 36.1 61.4 nm along two shift axes; -8.9 -72.3 nm on specimen axes Total_Shift = 37.409959 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.65 microns changed by -2.65 to target drift = 0.05 nm/sec Measured defocus = -1.00 microns changed by -0.00 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6129.tif 27 frames are being saved to U:\krios\20190717\PilA_6130.tif 27 frames are being saved to U:\krios\20190717\PilA_6131.tif 27 frames are being saved to U:\krios\20190717\PilA_6132.tif 27 frames are being saved to U:\krios\20190717\PilA_6133.tif 27 frames are being saved to U:\krios\20190717\PilA_6134.tif Running script 17 at item 149 (-580.14, 600.99) Remaining time to fill dewars is 22380 Dewars are NOT busy filling Trimming A by 23 195 20 240, reference by 210 22 20 230 Trimming A by 23 195 20 240, reference by 135 24 110 20 Trimming A by 23 195 20 240, reference by 20 182 20 240 Trimming A by 23 195 20 240, reference by 23 130 105 20 Disparity in stage position after aligning to center of map frame: 0.20 -0.18 Trimming A by 21 140 20 140, reference by 20 182 20 240 Disparity in stage position after aligning to target position: 0.32 -0.08 (2nd move error 0.11 0.09) Finished aligning to item in 2 rounds Trimming A by 23 135 20 135, reference by 21 140 20 140 -3.1 -1.8 unbinned pixels; 2.5 13.9 nm along two shift axes; 7.6 -11.8 nm on specimen axes Total_Shift = 7.174634 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.52 microns changed by -2.62 to target drift = 0.15 nm/sec Measured defocus = -1.06 microns changed by -0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6135.tif 27 frames are being saved to U:\krios\20190717\PilA_6136.tif 27 frames are being saved to U:\krios\20190717\PilA_6137.tif 27 frames are being saved to U:\krios\20190717\PilA_6138.tif 27 frames are being saved to U:\krios\20190717\PilA_6139.tif 27 frames are being saved to U:\krios\20190717\PilA_6140.tif Running script 17 at item 150 (-582.92, 606.50) Remaining time to fill dewars is 22200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 27 55 20 110, reference by 20 182 20 240 Disparity in stage position after aligning to target position: 0.24 -0.17 (2nd move error 0.03 -0.01) Finished aligning to item in 1 rounds Trimming A by 36 60 20 105, reference by 27 55 20 110 -3.3 -2.5 unbinned pixels; 5.0 15.8 nm along two shift axes; 10.6 -12.4 nm on specimen axes Total_Shift = 8.369586 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.45 microns changed by -2.65 to target drift = 0.14 nm/sec Measured defocus = -1.25 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6141.tif 27 frames are being saved to U:\krios\20190717\PilA_6142.tif 27 frames are being saved to U:\krios\20190717\PilA_6143.tif 27 frames are being saved to U:\krios\20190717\PilA_6144.tif 27 frames are being saved to U:\krios\20190717\PilA_6145.tif 27 frames are being saved to U:\krios\20190717\PilA_6146.tif Running script 17 at item 151 (-584.51, 609.23) Remaining time to fill dewars is 21960 Dewars are NOT busy filling Trimming A by 215 20 20 245, reference by 210 22 20 230 Trimming A by 215 20 20 245, reference by 135 24 110 20 Trimming A by 215 20 20 245, reference by 20 182 20 240 Trimming A by 215 20 20 245, reference by 23 130 105 20 Disparity in stage position after aligning to center of map frame: 0.27 -0.16 Trimming A by 86 23 20 170, reference by 210 22 20 230 Disparity in stage position after aligning to target position: 0.53 -0.17 (2nd move error 0.25 -0.03) Finished aligning to item in 2 rounds Trimming A by 74 22 20 170, reference by 86 23 20 170 -2.8 3.3 unbinned pixels; 15.8 4.9 nm along two shift axes; -12.3 -11.4 nm on specimen axes Total_Shift = 8.619548 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.44 microns changed by -2.74 to target drift = 0.07 nm/sec Measured defocus = -1.37 microns changed by 0.07 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6147.tif 27 frames are being saved to U:\krios\20190717\PilA_6148.tif 27 frames are being saved to U:\krios\20190717\PilA_6149.tif 27 frames are being saved to U:\krios\20190717\PilA_6150.tif 27 frames are being saved to U:\krios\20190717\PilA_6151.tif 27 frames are being saved to U:\krios\20190717\PilA_6152.tif Running script 17 at item 152 (-522.38, 562.77) Remaining time to fill dewars is 21780 Dewars are NOT busy filling Trimming A by 24 125 95 20, reference by 250 20 20 275 Trimming A by 24 125 95 20, reference by 175 24 125 20 Trimming A by 24 125 95 20, reference by 20 185 20 260 Trimming A by 24 125 95 20, reference by 24 125 95 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.24 Trimming A by 20 130 165 20, reference by 24 125 95 20 Disparity in stage position after aligning to target position: 0.03 -0.13 (2nd move error -0.06 0.10) Finished aligning to item in 2 rounds Trimming A by 22 117 175 20, reference by 20 130 165 20 3.2 13.5 unbinned pixels; 45.4 31.8 nm along two shift axes; -53.1 9.6 nm on specimen axes Total_Shift = 27.690043 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.77 microns changed by -2.17 to target drift = 0.16 nm/sec Measured defocus = -1.35 microns changed by -0.05 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6153.tif 27 frames are being saved to U:\krios\20190717\PilA_6154.tif 27 frames are being saved to U:\krios\20190717\PilA_6155.tif 27 frames are being saved to U:\krios\20190717\PilA_6156.tif 27 frames are being saved to U:\krios\20190717\PilA_6157.tif 27 frames are being saved to U:\krios\20190717\PilA_6158.tif Running script 17 at item 153 (-523.80, 565.51) Remaining time to fill dewars is 21600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 51 51 115 20, reference by 24 125 95 20 Disparity in stage position after aligning to target position: 0.04 -0.19 (2nd move error -0.05 0.02) Finished aligning to item in 1 rounds Trimming A by 51 51 115 20, reference by 51 51 115 20 0.7 10.4 unbinned pixels; 37.3 18.0 nm along two shift axes; -40.5 0.4 nm on specimen axes Total_Shift = 20.816294 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.67 microns changed by -2.17 to target drift = 0.12 nm/sec Measured defocus = -1.50 microns changed by -0.00 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6159.tif 27 frames are being saved to U:\krios\20190717\PilA_6160.tif 27 frames are being saved to U:\krios\20190717\PilA_6161.tif 27 frames are being saved to U:\krios\20190717\PilA_6162.tif 27 frames are being saved to U:\krios\20190717\PilA_6163.tif 27 frames are being saved to U:\krios\20190717\PilA_6164.tif Running script 17 at item 154 (-525.30, 568.25) Remaining time to fill dewars is 21360 Dewars are NOT busy filling Trimming A by 195 24 120 20, reference by 250 20 20 275 Trimming A by 195 24 120 20, reference by 175 24 125 20 Trimming A by 195 24 120 20, reference by 20 185 20 260 Trimming A by 195 24 120 20, reference by 24 125 95 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.22 Trimming A by 100 23 105 20, reference by 175 24 125 20 Disparity in stage position after aligning to target position: 0.11 -0.16 (2nd move error 0.03 0.06) Finished aligning to item in 2 rounds Trimming A by 86 20 120 20, reference by 100 23 105 20 -1.4 -1.4 unbinned pixels; 3.4 7.2 nm along two shift axes; 5.8 -5.2 nm on specimen axes Total_Shift = 4.0047 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.57 microns changed by -2.17 to target drift = 0.12 nm/sec Measured defocus = -1.53 microns changed by -0.07 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6165.tif 27 frames are being saved to U:\krios\20190717\PilA_6166.tif 27 frames are being saved to U:\krios\20190717\PilA_6167.tif 27 frames are being saved to U:\krios\20190717\PilA_6168.tif 27 frames are being saved to U:\krios\20190717\PilA_6169.tif 27 frames are being saved to U:\krios\20190717\PilA_6170.tif Running script 17 at item 155 (-526.71, 570.94) Remaining time to fill dewars is 21180 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 140 22 110 20, reference by 175 24 125 20 Disparity in stage position after aligning to target position: 0.14 -0.21 (2nd move error 0.06 -0.00) Finished aligning to item in 1 rounds Trimming A by 140 23 115 20, reference by 140 22 110 20 -0.9 1.3 unbinned pixels; 6.0 1.1 nm along two shift axes; -5.0 -3.6 nm on specimen axes Total_Shift = 3.179361 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.53 microns changed by -2.23 to target drift = 0.10 nm/sec Measured defocus = -1.73 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6171.tif 27 frames are being saved to U:\krios\20190717\PilA_6172.tif 27 frames are being saved to U:\krios\20190717\PilA_6173.tif 27 frames are being saved to U:\krios\20190717\PilA_6174.tif 27 frames are being saved to U:\krios\20190717\PilA_6175.tif 27 frames are being saved to U:\krios\20190717\PilA_6176.tif Running script 17 at item 156 (-530.84, 572.22) Remaining time to fill dewars is 21000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 20 25 95, reference by 175 24 125 20 Disparity in stage position after aligning to target position: 0.08 -0.22 (2nd move error -0.01 -0.01) Finished aligning to item in 1 rounds Trimming A by 145 20 25 95, reference by 145 20 25 95 -2.1 2.7 unbinned pixels; 12.6 3.6 nm along two shift axes; -10.0 -8.8 nm on specimen axes Total_Shift = 6.828008 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.53 microns changed by -2.33 to target drift = 0.05 nm/sec Measured defocus = -1.85 microns changed by 0.05 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6177.tif 27 frames are being saved to U:\krios\20190717\PilA_6178.tif 27 frames are being saved to U:\krios\20190717\PilA_6179.tif 27 frames are being saved to U:\krios\20190717\PilA_6180.tif 27 frames are being saved to U:\krios\20190717\PilA_6181.tif 27 frames are being saved to U:\krios\20190717\PilA_6182.tif Running script 17 at item 157 (-527.89, 566.70) Remaining time to fill dewars is 20760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 21 30 20, reference by 175 24 125 20 Disparity in stage position after aligning to target position: 0.24 -0.20 (2nd move error 0.16 0.01) Finished aligning to item in 1 rounds Trimming A by 40 20 35 20, reference by 35 21 30 20 1.5 -2.8 unbinned pixels; 12.3 1.3 nm along two shift axes; 10.6 6.5 nm on specimen axes Total_Shift = 6.38086 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.30 microns changed by -2.20 to target drift = 0.17 nm/sec Measured defocus = -1.81 microns changed by -0.09 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6183.tif 27 frames are being saved to U:\krios\20190717\PilA_6184.tif 27 frames are being saved to U:\krios\20190717\PilA_6185.tif 27 frames are being saved to U:\krios\20190717\PilA_6186.tif 27 frames are being saved to U:\krios\20190717\PilA_6187.tif 27 frames are being saved to U:\krios\20190717\PilA_6188.tif Running script 17 at item 158 (-526.38, 564.14) Remaining time to fill dewars is 20640 Dewars are NOT busy filling Trimming A by 24 125 95 20, reference by 250 20 20 275 Trimming A by 24 125 95 20, reference by 175 24 125 20 Trimming A by 24 125 95 20, reference by 20 185 20 260 Trimming A by 24 125 95 20, reference by 24 125 95 20 Disparity in stage position after aligning to center of map frame: 0.19 -0.24 Trimming A by 24 46 70 20, reference by 24 125 95 20 Disparity in stage position after aligning to target position: 0.08 -0.24 (2nd move error -0.12 -0.01) Finished aligning to item in 2 rounds Trimming A by 20 55 60 20, reference by 24 46 70 20 1.1 22.1 unbinned pixels; 79.8 37.2 nm along two shift axes; -86.2 -0.4 nm on specimen axes Total_Shift = 44.262346 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.23 microns changed by -2.23 to target drift = 0.10 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6189.tif 27 frames are being saved to U:\krios\20190717\PilA_6190.tif 27 frames are being saved to U:\krios\20190717\PilA_6191.tif 27 frames are being saved to U:\krios\20190717\PilA_6192.tif 27 frames are being saved to U:\krios\20190717\PilA_6193.tif 27 frames are being saved to U:\krios\20190717\PilA_6194.tif Running script 17 at item 159 (-525.02, 561.25) Remaining time to fill dewars is 20340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 115 125 20, reference by 24 125 95 20 Disparity in stage position after aligning to target position: 0.14 -0.21 (2nd move error -0.05 0.01) Finished aligning to item in 1 rounds Trimming A by 22 120 120 20, reference by 24 115 125 20 2.5 11.2 unbinned pixels; 38.0 25.8 nm along two shift axes; -44.1 7.3 nm on specimen axes Total_Shift = 22.946285 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.10 microns changed by -2.20 to target drift = 0.11 nm/sec Measured defocus = -2.06 microns changed by -0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6195.tif 27 frames are being saved to U:\krios\20190717\PilA_6196.tif 27 frames are being saved to U:\krios\20190717\PilA_6197.tif 27 frames are being saved to U:\krios\20190717\PilA_6198.tif 27 frames are being saved to U:\krios\20190717\PilA_6199.tif 27 frames are being saved to U:\krios\20190717\PilA_6200.tif Running script 17 at item 160 (-527.66, 559.90) Remaining time to fill dewars is 20100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 105 80 20, reference by 24 125 95 20 Disparity in stage position after aligning to target position: 0.22 -0.27 (2nd move error 0.03 -0.05) Finished aligning to item in 1 rounds Trimming A by 21 110 80 20, reference by 22 105 80 20 -7.2 -2.6 unbinned pixels; 0.0 29.7 nm along two shift axes; 11.5 -27.4 nm on specimen axes Total_Shift = 15.233083 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.15 microns changed by -2.35 to target drift = 0.13 nm/sec Measured defocus = -2.27 microns changed by 0.07 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6201.tif 27 frames are being saved to U:\krios\20190717\PilA_6202.tif 27 frames are being saved to U:\krios\20190717\PilA_6203.tif 27 frames are being saved to U:\krios\20190717\PilA_6204.tif 27 frames are being saved to U:\krios\20190717\PilA_6205.tif 27 frames are being saved to U:\krios\20190717\PilA_6206.tif Running script 17 at item 161 (-529.05, 562.68) Remaining time to fill dewars is 19920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 40 25 20, reference by 24 125 95 20 Disparity in stage position after aligning to target position: 0.08 -0.32 (2nd move error -0.13 -0.10) Finished aligning to item in 1 rounds Trimming A by 20 40 25 20, reference by 20 40 25 20 -12.4 38.3 unbinned pixels; 157.0 13.1 nm along two shift axes; -146.4 -56.2 nm on specimen axes Total_Shift = 80.495603 Trimming A by 20 40 25 20, reference by 20 40 25 20 -2.4 27.7 unbinned pixels; 105.2 32.9 nm along two shift axes; -107.4 -15.4 nm on specimen axes Total_Shift = 55.683759 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.03 microns changed by -2.33 to target drift = 0.03 nm/sec Measured defocus = -2.36 microns changed by 0.06 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6207.tif 27 frames are being saved to U:\krios\20190717\PilA_6208.tif 27 frames are being saved to U:\krios\20190717\PilA_6209.tif 27 frames are being saved to U:\krios\20190717\PilA_6210.tif 27 frames are being saved to U:\krios\20190717\PilA_6211.tif 27 frames are being saved to U:\krios\20190717\PilA_6212.tif Running script 17 at item 162 (-530.46, 565.49) Remaining time to fill dewars is 19740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 125 95 20 Disparity in stage position after aligning to target position: 0.05 -0.36 (2nd move error -0.14 -0.13) Finished aligning to item in 1 rounds -12.6 27.3 unbinned pixels; 117.0 4.3 nm along two shift axes; -103.7 -54.9 nm on specimen axes Total_Shift = 60.236973 -4.4 17.9 unbinned pixels; 71.6 11.1 nm along two shift axes; -68.8 -20.9 nm on specimen axes Total_Shift = 36.888252 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.02 microns changed by -2.38 to target drift = 0.00 nm/sec Measured defocus = -2.47 microns changed by 0.07 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6213.tif 27 frames are being saved to U:\krios\20190717\PilA_6214.tif 27 frames are being saved to U:\krios\20190717\PilA_6215.tif 27 frames are being saved to U:\krios\20190717\PilA_6216.tif 27 frames are being saved to U:\krios\20190717\PilA_6217.tif 27 frames are being saved to U:\krios\20190717\PilA_6218.tif Running script 17 at item 163 (-531.98, 568.16) Remaining time to fill dewars is 19620 Dewars are NOT busy filling Trimming A by 190 21 120 20, reference by 250 20 20 275 Trimming A by 190 21 120 20, reference by 175 24 125 20 Trimming A by 190 21 120 20, reference by 20 185 20 260 Trimming A by 190 21 120 20, reference by 24 125 95 20 Disparity in stage position after aligning to center of map frame: 0.18 -0.16 Trimming A by 70 20 20 75, reference by 175 24 125 20 Disparity in stage position after aligning to target position: 0.11 -0.27 (2nd move error -0.08 -0.13) Finished aligning to item in 2 rounds Trimming A by 70 20 20 75, reference by 70 20 20 75 -15.1 18.1 unbinned pixels; 86.2 27.2 nm along two shift axes; -67.1 -62.6 nm on specimen axes Total_Shift = 47.119254 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.19 microns changed by -2.31 to target drift = 0.02 nm/sec Measured defocus = -2.54 microns changed by 0.04 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6219.tif 27 frames are being saved to U:\krios\20190717\PilA_6220.tif 27 frames are being saved to U:\krios\20190717\PilA_6221.tif 27 frames are being saved to U:\krios\20190717\PilA_6222.tif 27 frames are being saved to U:\krios\20190717\PilA_6223.tif 27 frames are being saved to U:\krios\20190717\PilA_6224.tif Running script 17 at item 164 (-533.41, 570.78) Remaining time to fill dewars is 19380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 20 20 130, reference by 175 24 125 20 Disparity in stage position after aligning to target position: 0.04 -0.26 (2nd move error -0.15 -0.12) Finished aligning to item in 1 rounds Trimming A by 145 20 20 130, reference by 145 20 20 130 -12.1 30.4 unbinned pixels; 127.6 2.1 nm along two shift axes; -115.7 -53.6 nm on specimen axes Total_Shift = 65.44481 Trimming A by 145 21 70 125, reference by 145 20 20 130 -3.0 23.0 unbinned pixels; 88.7 23.8 nm along two shift axes; -89.0 -16.6 nm on specimen axes Total_Shift = 46.495094 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.28 microns changed by -2.32 to target drift = 0.02 nm/sec Measured defocus = -2.64 microns changed by 0.04 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6225.tif 27 frames are being saved to U:\krios\20190717\PilA_6226.tif 27 frames are being saved to U:\krios\20190717\PilA_6227.tif 27 frames are being saved to U:\krios\20190717\PilA_6228.tif 27 frames are being saved to U:\krios\20190717\PilA_6229.tif 27 frames are being saved to U:\krios\20190717\PilA_6230.tif Running script 17 at item 165 (-534.81, 566.51) Remaining time to fill dewars is 19140 Dewars are NOT busy filling Trimming A by 265 24 20 280, reference by 250 20 20 275 Trimming A by 265 24 20 280, reference by 175 24 125 20 Trimming A by 265 24 20 280, reference by 20 185 20 260 Trimming A by 265 24 20 280, reference by 24 125 95 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.12 Trimming A by 85 20 20 115, reference by 250 20 20 275 Disparity in stage position after aligning to target position: 0.49 -0.08 (2nd move error 0.35 0.03) Finished aligning to item in 2 rounds Trimming A by 57 20 20 125, reference by 85 20 20 115 -0.9 1.1 unbinned pixels; 5.2 1.8 nm along two shift axes; -4.0 -3.9 nm on specimen axes Total_Shift = 2.870213 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.56 microns changed by -2.46 to target drift = 0.15 nm/sec Measured defocus = -0.87 microns changed by -0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6231.tif 27 frames are being saved to U:\krios\20190717\PilA_6232.tif 27 frames are being saved to U:\krios\20190717\PilA_6233.tif 27 frames are being saved to U:\krios\20190717\PilA_6234.tif 27 frames are being saved to U:\krios\20190717\PilA_6235.tif 27 frames are being saved to U:\krios\20190717\PilA_6236.tif Running script 17 at item 166 (-533.01, 563.99) Remaining time to fill dewars is 18960 Dewars are NOT busy filling Trimming A by 24 125 95 20, reference by 250 20 20 275 Trimming A by 24 125 95 20, reference by 175 24 125 20 Trimming A by 24 125 95 20, reference by 20 185 20 260 Trimming A by 24 125 95 20, reference by 24 125 95 20 Disparity in stage position after aligning to center of map frame: 0.18 -0.17 Trimming A by 20 26 20 80, reference by 24 125 95 20 Disparity in stage position after aligning to target position: -0.01 -0.36 (2nd move error -0.19 -0.22) Finished aligning to item in 2 rounds Trimming A by 21 50 20 65, reference by 20 26 20 80 -21.8 36.8 unbinned pixels; 163.7 23.2 nm along two shift axes; -138.5 -92.6 nm on specimen axes Total_Shift = 85.534867 Trimming A by 22 32 20 85, reference by 20 26 20 80 1.6 23.9 unbinned pixels; 85.8 41.9 nm along two shift axes; -93.4 1.3 nm on specimen axes Total_Shift = 47.954013 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.44 microns changed by -2.44 to target drift = 0.02 nm/sec Measured defocus = -1.01 microns changed by 0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6237.tif 27 frames are being saved to U:\krios\20190717\PilA_6238.tif 27 frames are being saved to U:\krios\20190717\PilA_6239.tif 27 frames are being saved to U:\krios\20190717\PilA_6240.tif 27 frames are being saved to U:\krios\20190717\PilA_6241.tif 27 frames are being saved to U:\krios\20190717\PilA_6242.tif Running script 17 at item 167 (-531.72, 561.23) Remaining time to fill dewars is 18660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 75 20 80, reference by 24 125 95 20 Disparity in stage position after aligning to target position: 0.08 -0.38 (2nd move error -0.10 -0.23) Finished aligning to item in 1 rounds Trimming A by 20 80 20 80, reference by 21 75 20 80 -23.1 20.5 unbinned pixels; 105.5 52.3 nm along two shift axes; -74.8 -94.2 nm on specimen axes Total_Shift = 61.74701 Trimming A by 24 85 20 80, reference by 21 75 20 80 0.7 12.8 unbinned pixels; 46.0 21.8 nm along two shift axes; -49.8 0.1 nm on specimen axes Total_Shift = 25.557794 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.38 microns changed by -2.48 to target drift = 0.03 nm/sec Measured defocus = -1.13 microns changed by 0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6243.tif 27 frames are being saved to U:\krios\20190717\PilA_6244.tif 27 frames are being saved to U:\krios\20190717\PilA_6245.tif 27 frames are being saved to U:\krios\20190717\PilA_6246.tif 27 frames are being saved to U:\krios\20190717\PilA_6247.tif 27 frames are being saved to U:\krios\20190717\PilA_6248.tif Running script 17 at item 168 (-530.29, 558.51) Remaining time to fill dewars is 18480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 130 20 90, reference by 24 125 95 20 Disparity in stage position after aligning to target position: 0.16 -0.30 (2nd move error -0.02 -0.16) Finished aligning to item in 1 rounds Trimming A by 21 130 20 90, reference by 21 130 20 90 -21.8 6.4 unbinned pixels; 51.9 68.7 nm along two shift axes; -20.2 -86.0 nm on specimen axes Total_Shift = 45.358797 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.18 microns changed by -2.38 to target drift = 0.04 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6249.tif 27 frames are being saved to U:\krios\20190717\PilA_6250.tif 27 frames are being saved to U:\krios\20190717\PilA_6251.tif 27 frames are being saved to U:\krios\20190717\PilA_6252.tif 27 frames are being saved to U:\krios\20190717\PilA_6253.tif 27 frames are being saved to U:\krios\20190717\PilA_6254.tif Running script 17 at item 169 (-534.45, 559.56) Remaining time to fill dewars is 18300 Dewars are NOT busy filling Trimming A by 22 185 20 270, reference by 250 20 20 275 Trimming A by 22 185 20 270, reference by 175 24 125 20 Trimming A by 22 185 20 270, reference by 20 185 20 260 Trimming A by 22 185 20 270, reference by 24 125 95 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.17 Trimming A by 23 150 20 155, reference by 20 185 20 260 Disparity in stage position after aligning to target position: 0.29 -0.02 (2nd move error 0.15 0.14) Finished aligning to item in 2 rounds Trimming A by 23 127 20 165, reference by 23 150 20 155 0.2 3.8 unbinned pixels; 13.8 6.5 nm along two shift axes; -14.9 0.0 nm on specimen axes Total_Shift = 7.665036 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.23 microns changed by -2.53 to target drift = 0.12 nm/sec Measured defocus = -1.24 microns changed by -0.06 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6255.tif 27 frames are being saved to U:\krios\20190717\PilA_6256.tif 27 frames are being saved to U:\krios\20190717\PilA_6257.tif 27 frames are being saved to U:\krios\20190717\PilA_6258.tif 27 frames are being saved to U:\krios\20190717\PilA_6259.tif 27 frames are being saved to U:\krios\20190717\PilA_6260.tif Running script 17 at item 170 (-535.81, 562.28) Remaining time to fill dewars is 18120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 105 20 150, reference by 20 185 20 260 Disparity in stage position after aligning to target position: 0.22 -0.13 (2nd move error 0.08 0.03) Finished aligning to item in 1 rounds Trimming A by 21 91 20 160, reference by 21 105 20 150 -0.5 -3.6 unbinned pixels; 12.5 7.0 nm along two shift axes; 13.9 -1.0 nm on specimen axes Total_Shift = 7.179637 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.12 microns changed by -2.52 to target drift = 0.12 nm/sec Measured defocus = -1.39 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6261.tif 27 frames are being saved to U:\krios\20190717\PilA_6262.tif 27 frames are being saved to U:\krios\20190717\PilA_6263.tif 27 frames are being saved to U:\krios\20190717\PilA_6264.tif 27 frames are being saved to U:\krios\20190717\PilA_6265.tif 27 frames are being saved to U:\krios\20190717\PilA_6266.tif Running script 17 at item 171 (-263.17, 588.72) Remaining time to fill dewars is 17940 Dewars are NOT busy filling Trimming A by 125 20 130 20, reference by 162 21 20 185 Trimming A by 125 20 130 20, reference by 116 21 125 20 Trimming A by 125 20 130 20, reference by 23 122 20 150 Trimming A by 125 20 130 20, reference by 21 87 115 20 Disparity in stage position after aligning to center of map frame: -0.01 -0.15 Trimming A by 130 21 170 20, reference by 116 21 125 20 Disparity in stage position after aligning to target position: 0.11 -0.10 (2nd move error 0.11 0.04) Finished aligning to item in 2 rounds Trimming A by 131 24 175 20, reference by 130 21 170 20 -0.2 3.5 unbinned pixels; 13.2 4.4 nm along two shift axes; -13.6 -1.7 nm on specimen axes Total_Shift = 7.037983 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.76 microns changed by -2.26 to target drift = 0.22 nm/sec Measured defocus = -1.47 microns changed by -0.03 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6267.tif 27 frames are being saved to U:\krios\20190717\PilA_6268.tif 27 frames are being saved to U:\krios\20190717\PilA_6269.tif 27 frames are being saved to U:\krios\20190717\PilA_6270.tif 27 frames are being saved to U:\krios\20190717\PilA_6271.tif 27 frames are being saved to U:\krios\20190717\PilA_6272.tif Running script 17 at item 172 (-267.27, 590.02) Remaining time to fill dewars is 17700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 107 24 115 20, reference by 116 21 125 20 Disparity in stage position after aligning to target position: 0.11 -0.19 (2nd move error 0.11 -0.05) Finished aligning to item in 1 rounds Trimming A by 125 23 100 20, reference by 107 24 115 20 -5.5 1.6 unbinned pixels; 12.9 17.5 nm along two shift axes; -4.9 -21.8 nm on specimen axes Total_Shift = 11.454305 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.81 microns changed by -2.41 to target drift = 0.06 nm/sec Measured defocus = -1.58 microns changed by -0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6273.tif 27 frames are being saved to U:\krios\20190717\PilA_6274.tif 27 frames are being saved to U:\krios\20190717\PilA_6275.tif 27 frames are being saved to U:\krios\20190717\PilA_6276.tif 27 frames are being saved to U:\krios\20190717\PilA_6277.tif 27 frames are being saved to U:\krios\20190717\PilA_6278.tif Running script 17 at item 173 (-269.83, 588.69) Remaining time to fill dewars is 17520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 60 20 35 50, reference by 116 21 125 20 Disparity in stage position after aligning to target position: 0.09 -0.26 (2nd move error 0.09 -0.12) Finished aligning to item in 1 rounds Trimming A by 60 20 35 45, reference by 60 20 35 50 -14.8 1.0 unbinned pixels; 23.0 52.0 nm along two shift axes; -0.7 -58.0 nm on specimen axes Total_Shift = 29.760079 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.69 microns changed by -2.38 to target drift = 0.09 nm/sec Measured defocus = -1.75 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6279.tif 27 frames are being saved to U:\krios\20190717\PilA_6280.tif 27 frames are being saved to U:\krios\20190717\PilA_6281.tif 27 frames are being saved to U:\krios\20190717\PilA_6282.tif 27 frames are being saved to U:\krios\20190717\PilA_6283.tif 27 frames are being saved to U:\krios\20190717\PilA_6284.tif Running script 17 at item 174 (-271.28, 591.42) Remaining time to fill dewars is 17280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 20 20 100, reference by 116 21 125 20 Disparity in stage position after aligning to target position: 0.01 -0.21 (2nd move error 0.00 -0.07) Finished aligning to item in 1 rounds Trimming A by 140 20 20 100, reference by 140 20 20 100 -9.8 0.7 unbinned pixels; 15.5 34.4 nm along two shift axes; -0.7 -38.5 nm on specimen axes Total_Shift = 19.74736 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.60 microns changed by -2.40 to target drift = 0.04 nm/sec Measured defocus = -1.89 microns changed by 0.09 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6285.tif 27 frames are being saved to U:\krios\20190717\PilA_6286.tif 27 frames are being saved to U:\krios\20190717\PilA_6287.tif 27 frames are being saved to U:\krios\20190717\PilA_6288.tif 27 frames are being saved to U:\krios\20190717\PilA_6289.tif 27 frames are being saved to U:\krios\20190717\PilA_6290.tif Running script 17 at item 175 (-272.45, 587.03) Remaining time to fill dewars is 17160 Dewars are NOT busy filling Trimming A by 185 24 35 180, reference by 162 21 20 185 Trimming A by 185 24 35 180, reference by 116 21 125 20 Trimming A by 185 24 35 180, reference by 23 122 20 150 Trimming A by 185 24 35 180, reference by 21 87 115 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.05 Trimming A by 70 20 20 70, reference by 162 21 20 185 Disparity in stage position after aligning to target position: 0.27 -0.01 (2nd move error 0.15 0.03) Finished aligning to item in 2 rounds Trimming A by 70 20 20 70, reference by 70 20 20 70 -0.7 -1.0 unbinned pixels; 2.7 4.0 nm along two shift axes; 4.0 -2.5 nm on specimen axes Total_Shift = 2.430996 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.35 microns changed by -2.25 to target drift = 0.11 nm/sec Measured defocus = -1.85 microns changed by -0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6291.tif 27 frames are being saved to U:\krios\20190717\PilA_6292.tif 27 frames are being saved to U:\krios\20190717\PilA_6293.tif 27 frames are being saved to U:\krios\20190717\PilA_6294.tif 27 frames are being saved to U:\krios\20190717\PilA_6295.tif 27 frames are being saved to U:\krios\20190717\PilA_6296.tif Running script 17 at item 176 (-277.72, 584.17) Remaining time to fill dewars is 16920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 106 22 20 130, reference by 162 21 20 185 Disparity in stage position after aligning to target position: 0.27 -0.10 (2nd move error 0.16 -0.07) Finished aligning to item in 1 rounds Trimming A by 81 21 20 140, reference by 106 22 20 130 -7.0 2.8 unbinned pixels; 19.2 20.9 nm along two shift axes; -9.2 -27.7 nm on specimen axes Total_Shift = 14.969705 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.50 microns changed by -2.50 to target drift = 0.10 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6297.tif 27 frames are being saved to U:\krios\20190717\PilA_6298.tif 27 frames are being saved to U:\krios\20190717\PilA_6299.tif 27 frames are being saved to U:\krios\20190717\PilA_6300.tif 27 frames are being saved to U:\krios\20190717\PilA_6301.tif 27 frames are being saved to U:\krios\20190717\PilA_6302.tif Running script 17 at item 177 (-276.18, 581.49) Remaining time to fill dewars is 16800 Dewars are NOT busy filling Trimming A by 22 145 20 140, reference by 162 21 20 185 Trimming A by 22 145 20 140, reference by 116 21 125 20 Trimming A by 22 145 20 140, reference by 23 122 20 150 Trimming A by 22 145 20 140, reference by 21 87 115 20 Disparity in stage position after aligning to center of map frame: 0.16 -0.12 Trimming A by 45 21 20 55, reference by 23 122 20 150 Disparity in stage position after aligning to target position: 0.06 -0.04 (2nd move error -0.11 0.07) Finished aligning to item in 2 rounds Trimming A by 31 21 20 70, reference by 45 21 20 55 1.6 21.9 unbinned pixels; 78.3 38.8 nm along two shift axes; -85.4 1.7 nm on specimen axes Total_Shift = 43.869518 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.36 microns changed by -2.45 to target drift = 0.15 nm/sec Measured defocus = -2.10 microns changed by 0.00 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6303.tif 27 frames are being saved to U:\krios\20190717\PilA_6304.tif 27 frames are being saved to U:\krios\20190717\PilA_6305.tif 27 frames are being saved to U:\krios\20190717\PilA_6306.tif 27 frames are being saved to U:\krios\20190717\PilA_6307.tif 27 frames are being saved to U:\krios\20190717\PilA_6308.tif Running script 17 at item 178 (-273.33, 575.92) Remaining time to fill dewars is 16500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 65 20 80, reference by 23 122 20 150 Disparity in stage position after aligning to target position: 0.26 -0.02 (2nd move error 0.10 0.08) Finished aligning to item in 1 rounds Trimming A by 20 65 20 75, reference by 21 65 20 80 -0.2 0.5 unbinned pixels; 2.1 0.1 nm along two shift axes; -1.8 -1.0 nm on specimen axes Total_Shift = 1.06276 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.51 microns changed by -2.71 to target drift = 0.13 nm/sec Measured defocus = -2.25 microns changed by 0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6309.tif 27 frames are being saved to U:\krios\20190717\PilA_6310.tif 27 frames are being saved to U:\krios\20190717\PilA_6311.tif 27 frames are being saved to U:\krios\20190717\PilA_6312.tif 27 frames are being saved to U:\krios\20190717\PilA_6313.tif 27 frames are being saved to U:\krios\20190717\PilA_6314.tif Running script 17 at item 179 (-277.38, 577.29) Remaining time to fill dewars is 16320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 110 20 120, reference by 23 122 20 150 Disparity in stage position after aligning to target position: 0.18 -0.12 (2nd move error 0.01 -0.02) Finished aligning to item in 1 rounds Trimming A by 24 96 20 130, reference by 22 110 20 120 -4.5 -0.6 unbinned pixels; 3.7 17.1 nm along two shift axes; 3.3 -17.3 nm on specimen axes Total_Shift = 9.060632 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.27 microns changed by -2.57 to target drift = 0.09 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6315.tif 27 frames are being saved to U:\krios\20190717\PilA_6316.tif 27 frames are being saved to U:\krios\20190717\PilA_6317.tif 27 frames are being saved to U:\krios\20190717\PilA_6318.tif 27 frames are being saved to U:\krios\20190717\PilA_6319.tif 27 frames are being saved to U:\krios\20190717\PilA_6320.tif Running script 17 at item 181 (-271.95, 573.34) Remaining time to fill dewars is 16140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 81 20 105, reference by 23 122 20 150 Disparity in stage position after aligning to target position: 0.30 -0.03 (2nd move error 0.14 0.07) Finished aligning to item in 1 rounds Trimming A by 20 85 20 95, reference by 24 81 20 105 0.9 1.1 unbinned pixels; 2.7 4.9 nm along two shift axes; -4.3 3.4 nm on specimen axes Total_Shift = 2.81299 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.20 microns changed by -2.60 to target drift = 0.14 nm/sec Measured defocus = -2.34 microns changed by -0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6321.tif 27 frames are being saved to U:\krios\20190717\PilA_6322.tif 27 frames are being saved to U:\krios\20190717\PilA_6323.tif 27 frames are being saved to U:\krios\20190717\PilA_6324.tif 27 frames are being saved to U:\krios\20190717\PilA_6325.tif 27 frames are being saved to U:\krios\20190717\PilA_6326.tif Running script 17 at item 182 (-270.63, 570.53) Remaining time to fill dewars is 15900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 140 20 115, reference by 23 122 20 150 Disparity in stage position after aligning to target position: 0.44 0.01 (2nd move error 0.28 0.11) Finished aligning to item in 1 rounds Trimming A by 21 140 75 100, reference by 20 140 20 115 0.8 2.2 unbinned pixels; 7.1 6.0 nm along two shift axes; -8.7 2.5 nm on specimen axes Total_Shift = 4.659221 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -1.21 microns changed by -1.29 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6327.tif 27 frames are being saved to U:\krios\20190717\PilA_6328.tif 27 frames are being saved to U:\krios\20190717\PilA_6329.tif 27 frames are being saved to U:\krios\20190717\PilA_6330.tif 27 frames are being saved to U:\krios\20190717\PilA_6331.tif 27 frames are being saved to U:\krios\20190717\PilA_6332.tif Running script 17 at item 183 (-268.21, 572.16) Remaining time to fill dewars is 15660 Dewars are NOT busy filling Trimming A by 22 100 100 20, reference by 162 21 20 185 Trimming A by 22 100 100 20, reference by 116 21 125 20 Trimming A by 22 100 100 20, reference by 23 122 20 150 Trimming A by 22 100 100 20, reference by 21 87 115 20 Disparity in stage position after aligning to center of map frame: 0.40 -0.11 Trimming A by 21 100 70 20, reference by 21 87 115 20 Disparity in stage position after aligning to target position: 0.52 -0.23 (2nd move error 0.11 -0.13) Finished aligning to item in 2 rounds Trimming A by 21 105 70 20, reference by 21 100 70 20 -15.2 -3.0 unbinned pixels; 9.0 59.2 nm along two shift axes; 14.7 -58.6 nm on specimen axes Total_Shift = 31.015079 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.07 microns changed by -2.53 to target drift = 0.16 nm/sec Measured defocus = -2.67 microns changed by 0.07 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6333.tif 27 frames are being saved to U:\krios\20190717\PilA_6334.tif 27 frames are being saved to U:\krios\20190717\PilA_6335.tif 27 frames are being saved to U:\krios\20190717\PilA_6336.tif 27 frames are being saved to U:\krios\20190717\PilA_6337.tif 27 frames are being saved to U:\krios\20190717\PilA_6338.tif Running script 17 at item 184 (-266.78, 569.52) Remaining time to fill dewars is 15480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 175 120 20, reference by 21 87 115 20 Disparity in stage position after aligning to target position: 0.55 -0.17 (2nd move error 0.14 -0.09) Finished aligning to item in 1 rounds Trimming A by 24 180 120 20, reference by 22 175 120 20 -9.8 -0.4 unbinned pixels; 11.4 35.8 nm along two shift axes; 3.5 -38.0 nm on specimen axes Total_Shift = 19.583041 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 0.36 microns changed by -1.26 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6339.tif 27 frames are being saved to U:\krios\20190717\PilA_6340.tif 27 frames are being saved to U:\krios\20190717\PilA_6341.tif 27 frames are being saved to U:\krios\20190717\PilA_6342.tif 27 frames are being saved to U:\krios\20190717\PilA_6343.tif 27 frames are being saved to U:\krios\20190717\PilA_6344.tif Running script 17 at item 185 (-213.30, 539.18) Remaining time to fill dewars is 15360 Dewars are NOT busy filling Trimming A by 24 80 80 20, reference by 220 21 20 215 Trimming A by 24 80 80 20, reference by 156 23 145 20 Trimming A by 24 80 80 20, reference by 20 126 20 175 Trimming A by 24 80 80 20, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.21 Trimming A by 80 80 145 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.05 -0.03 (2nd move error -0.04 0.16) Finished aligning to item in 2 rounds Trimming A by 75 72 150 20, reference by 80 80 145 20 4.0 9.1 unbinned pixels; 28.0 28.1 nm along two shift axes; -36.1 13.7 nm on specimen axes Total_Shift = 19.814433 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.15 microns changed by -2.15 to target drift = 0.22 nm/sec Measured defocus = -0.94 microns changed by -0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6345.tif 27 frames are being saved to U:\krios\20190717\PilA_6346.tif 27 frames are being saved to U:\krios\20190717\PilA_6347.tif 27 frames are being saved to U:\krios\20190717\PilA_6348.tif 27 frames are being saved to U:\krios\20190717\PilA_6349.tif 27 frames are being saved to U:\krios\20190717\PilA_6350.tif Running script 17 at item 186 (-214.92, 541.92) Remaining time to fill dewars is 15120 Dewars are NOT busy filling Trimming A by 180 23 140 20, reference by 220 21 20 215 Trimming A by 180 23 140 20, reference by 156 23 145 20 Trimming A by 180 23 140 20, reference by 20 126 20 175 Trimming A by 180 23 140 20, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.18 Trimming A by 105 23 150 20, reference by 156 23 145 20 Disparity in stage position after aligning to target position: 0.15 -0.03 (2nd move error 0.09 0.14) Finished aligning to item in 2 rounds Trimming A by 106 21 155 20, reference by 105 23 150 20 -0.0 0.3 unbinned pixels; 1.0 0.3 nm along two shift axes; -1.0 -0.1 nm on specimen axes Total_Shift = 0.512157 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.07 microns changed by -2.17 to target drift = 0.13 nm/sec Measured defocus = -1.04 microns changed by -0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6351.tif 27 frames are being saved to U:\krios\20190717\PilA_6352.tif 27 frames are being saved to U:\krios\20190717\PilA_6353.tif 27 frames are being saved to U:\krios\20190717\PilA_6354.tif 27 frames are being saved to U:\krios\20190717\PilA_6355.tif 27 frames are being saved to U:\krios\20190717\PilA_6356.tif Running script 17 at item 187 (-221.74, 548.70) Remaining time to fill dewars is 14880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 170 24 115 20, reference by 156 23 145 20 Disparity in stage position after aligning to target position: 0.04 -0.15 (2nd move error -0.03 0.00) Finished aligning to item in 1 rounds Trimming A by 170 24 115 20, reference by 170 24 115 20 0.9 5.9 unbinned pixels; 20.6 11.9 nm along two shift axes; -23.1 2.1 nm on specimen axes Total_Shift = 11.916459 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.07 microns changed by -2.27 to target drift = 0.11 nm/sec Measured defocus = -1.14 microns changed by -0.06 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6357.tif 27 frames are being saved to U:\krios\20190717\PilA_6358.tif 27 frames are being saved to U:\krios\20190717\PilA_6359.tif 27 frames are being saved to U:\krios\20190717\PilA_6360.tif 27 frames are being saved to U:\krios\20190717\PilA_6361.tif 27 frames are being saved to U:\krios\20190717\PilA_6362.tif Running script 17 at item 188 (-220.28, 545.91) Remaining time to fill dewars is 14700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 120 21 105 20, reference by 156 23 145 20 Disparity in stage position after aligning to target position: 0.12 -0.13 (2nd move error 0.06 0.03) Finished aligning to item in 1 rounds Trimming A by 125 24 105 20, reference by 120 21 105 20 -0.5 -2.9 unbinned pixels; 10.0 6.2 nm along two shift axes; 11.4 -1.4 nm on specimen axes Total_Shift = 5.89026 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.96 microns changed by -2.26 to target drift = 0.13 nm/sec Measured defocus = -1.28 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6363.tif 27 frames are being saved to U:\krios\20190717\PilA_6364.tif 27 frames are being saved to U:\krios\20190717\PilA_6365.tif 27 frames are being saved to U:\krios\20190717\PilA_6366.tif 27 frames are being saved to U:\krios\20190717\PilA_6367.tif 27 frames are being saved to U:\krios\20190717\PilA_6368.tif Running script 17 at item 189 (-218.91, 543.22) Remaining time to fill dewars is 14580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 23 80 20, reference by 156 23 145 20 Disparity in stage position after aligning to target position: 0.25 -0.14 (2nd move error 0.18 0.01) Finished aligning to item in 1 rounds Trimming A by 90 23 85 20, reference by 90 23 80 20 4.1 5.9 unbinned pixels; 16.2 23.6 nm along two shift axes; -23.7 14.7 nm on specimen axes Total_Shift = 14.303659 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 0.82 microns changed by -2.22 to target drift = 0.09 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6369.tif 27 frames are being saved to U:\krios\20190717\PilA_6370.tif 27 frames are being saved to U:\krios\20190717\PilA_6371.tif 27 frames are being saved to U:\krios\20190717\PilA_6372.tif 27 frames are being saved to U:\krios\20190717\PilA_6373.tif 27 frames are being saved to U:\krios\20190717\PilA_6374.tif Running script 17 at item 190 (-217.33, 540.51) Remaining time to fill dewars is 14400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 47 22 80 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.03 -0.08 (2nd move error -0.06 0.11) Finished aligning to item in 1 rounds Trimming A by 55 22 75 20, reference by 47 22 80 20 -1.4 13.5 unbinned pixels; 51.6 15.4 nm along two shift axes; -52.4 -8.2 nm on specimen axes Total_Shift = 27.216487 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 0.72 microns changed by -2.22 to target drift = 0.14 nm/sec Measured defocus = -1.50 microns changed by 0.00 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6375.tif 27 frames are being saved to U:\krios\20190717\PilA_6376.tif 27 frames are being saved to U:\krios\20190717\PilA_6377.tif 27 frames are being saved to U:\krios\20190717\PilA_6378.tif 27 frames are being saved to U:\krios\20190717\PilA_6379.tif 27 frames are being saved to U:\krios\20190717\PilA_6380.tif Running script 17 at item 191 (-215.90, 537.73) Remaining time to fill dewars is 14160 Dewars are NOT busy filling Trimming A by 20 75 80 20, reference by 220 21 20 215 Trimming A by 20 75 80 20, reference by 156 23 145 20 Trimming A by 20 75 80 20, reference by 20 126 20 175 Trimming A by 20 75 80 20, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.19 -0.24 Trimming A by 24 62 110 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.16 -0.09 (2nd move error -0.05 0.13) Finished aligning to item in 2 rounds Trimming A by 24 57 110 20, reference by 24 62 110 20 -0.1 10.7 unbinned pixels; 39.5 15.7 nm along two shift axes; -41.6 -2.7 nm on specimen axes Total_Shift = 21.393291 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.59 microns changed by -2.19 to target drift = 0.14 nm/sec Measured defocus = -1.59 microns changed by -0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6381.tif 27 frames are being saved to U:\krios\20190717\PilA_6382.tif 27 frames are being saved to U:\krios\20190717\PilA_6383.tif 27 frames are being saved to U:\krios\20190717\PilA_6384.tif 27 frames are being saved to U:\krios\20190717\PilA_6385.tif 27 frames are being saved to U:\krios\20190717\PilA_6386.tif Running script 17 at item 192 (-217.19, 533.57) Remaining time to fill dewars is 14040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 125 115 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.18 -0.18 (2nd move error -0.02 0.04) Finished aligning to item in 1 rounds Trimming A by 24 125 115 20, reference by 24 125 115 20 0.5 4.2 unbinned pixels; 14.8 8.3 nm along two shift axes; -16.5 1.2 nm on specimen axes Total_Shift = 8.489705 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.56 microns changed by -2.26 to target drift = 0.13 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6387.tif 27 frames are being saved to U:\krios\20190717\PilA_6388.tif 27 frames are being saved to U:\krios\20190717\PilA_6389.tif 27 frames are being saved to U:\krios\20190717\PilA_6390.tif 27 frames are being saved to U:\krios\20190717\PilA_6391.tif 27 frames are being saved to U:\krios\20190717\PilA_6392.tif Running script 17 at item 193 (-218.58, 536.32) Remaining time to fill dewars is 13740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 50 65 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.16 -0.20 (2nd move error -0.03 0.02) Finished aligning to item in 1 rounds Trimming A by 20 60 55 20, reference by 22 50 65 20 0.7 6.9 unbinned pixels; 24.5 12.9 nm along two shift axes; -27.1 1.2 nm on specimen axes Total_Shift = 13.91155 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.44 microns changed by -2.24 to target drift = 0.14 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6393.tif 27 frames are being saved to U:\krios\20190717\PilA_6394.tif 27 frames are being saved to U:\krios\20190717\PilA_6395.tif 27 frames are being saved to U:\krios\20190717\PilA_6396.tif 27 frames are being saved to U:\krios\20190717\PilA_6397.tif 27 frames are being saved to U:\krios\20190717\PilA_6398.tif Running script 17 at item 194 (-219.98, 539.14) Remaining time to fill dewars is 13560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.05 -0.21 (2nd move error -0.14 0.01) Finished aligning to item in 1 rounds 3.8 28.4 unbinned pixels; 99.4 56.5 nm along two shift axes; -111.3 8.9 nm on specimen axes Total_Shift = 57.307709 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.31 microns changed by -2.21 to target drift = 0.10 nm/sec Measured defocus = -1.84 microns changed by -0.06 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6399.tif 27 frames are being saved to U:\krios\20190717\PilA_6400.tif 27 frames are being saved to U:\krios\20190717\PilA_6401.tif 27 frames are being saved to U:\krios\20190717\PilA_6402.tif 27 frames are being saved to U:\krios\20190717\PilA_6403.tif 27 frames are being saved to U:\krios\20190717\PilA_6404.tif Running script 17 at item 195 (-221.55, 541.87) Remaining time to fill dewars is 13380 Dewars are NOT busy filling Trimming A by 175 20 140 20, reference by 220 21 20 215 Trimming A by 175 20 140 20, reference by 156 23 145 20 Trimming A by 175 20 140 20, reference by 20 126 20 175 Trimming A by 175 20 140 20, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.52 0.06 Trimming A by 35 21 30 20, reference by 156 23 145 20 Disparity in stage position after aligning to target position: 0.18 -0.16 (2nd move error -0.34 -0.23) Finished aligning to item in 2 rounds Trimming A by 30 20 30 20, reference by 35 21 30 20 -21.1 67.5 unbinned pixels; 275.7 25.8 nm along two shift axes; -258.1 -96.1 nm on specimen axes Total_Shift = 141.405989 Trimming A by 30 20 25 20, reference by 35 21 30 20 5.3 43.1 unbinned pixels; 151.6 84.1 nm along two shift axes; -168.9 11.7 nm on specimen axes Total_Shift = 86.920158 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.36 microns changed by -2.36 to target drift = 0.03 nm/sec Measured defocus = -2.10 microns changed by 0.10 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6405.tif 27 frames are being saved to U:\krios\20190717\PilA_6406.tif 27 frames are being saved to U:\krios\20190717\PilA_6407.tif 27 frames are being saved to U:\krios\20190717\PilA_6408.tif 27 frames are being saved to U:\krios\20190717\PilA_6409.tif 27 frames are being saved to U:\krios\20190717\PilA_6410.tif Running script 17 at item 196 (-222.92, 544.58) Remaining time to fill dewars is 13200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 24 50 20, reference by 156 23 145 20 Disparity in stage position after aligning to target position: 0.18 -0.16 (2nd move error -0.35 -0.24) Finished aligning to item in 1 rounds Trimming A by 65 24 50 20, reference by 70 24 50 20 -24.5 67.9 unbinned pixels; 281.6 13.9 nm along two shift axes; -258.9 -109.7 nm on specimen axes Total_Shift = 144.365041 Trimming A by 60 20 50 20, reference by 70 24 50 20 -0.7 45.8 unbinned pixels; 169.3 66.5 nm along two shift axes; -178.1 -12.3 nm on specimen axes Total_Shift = 91.630647 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.20 microns changed by -2.30 to target drift = 0.03 nm/sec Measured defocus = -2.11 microns changed by 0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6411.tif 27 frames are being saved to U:\krios\20190717\PilA_6412.tif 27 frames are being saved to U:\krios\20190717\PilA_6413.tif 27 frames are being saved to U:\krios\20190717\PilA_6414.tif 27 frames are being saved to U:\krios\20190717\PilA_6415.tif 27 frames are being saved to U:\krios\20190717\PilA_6416.tif Running script 17 at item 197 (-224.30, 547.21) Remaining time to fill dewars is 12960 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 126 20 60 45, reference by 156 23 145 20 Disparity in stage position after aligning to target position: 0.02 -0.20 (2nd move error -0.50 -0.26) Finished aligning to item in 1 rounds Trimming A by 110 20 55 65, reference by 126 20 60 45 -11.8 97.2 unbinned pixels; 372.8 103.9 nm along two shift axes; -375.7 -66.3 nm on specimen axes Total_Shift = 195.820717 Trimming A by 110 20 50 65, reference by 126 20 60 45 10.7 61.6 unbinned pixels; 212.4 131.0 nm along two shift axes; -241.7 28.5 nm on specimen axes Total_Shift = 124.946178 Total_shift never < 120: Skiping ................................................ Running script 17 at item 198 (-227.07, 545.63) Remaining time to fill dewars is 12960 Dewars are NOT busy filling Trimming A by 220 21 20 230, reference by 220 21 20 215 Trimming A by 220 21 20 230, reference by 156 23 145 20 Trimming A by 220 21 20 230, reference by 20 126 20 175 Trimming A by 220 21 20 230, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.06 Trimming A by 120 23 20 115, reference by 220 21 20 215 Disparity in stage position after aligning to target position: 0.34 0.03 (2nd move error 0.23 0.07) Finished aligning to item in 2 rounds Trimming A by 120 22 20 110, reference by 120 23 20 115 0.5 -6.4 unbinned pixels; 24.1 7.9 nm along two shift axes; 24.7 3.2 nm on specimen axes Total_Shift = 12.791452 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.19 microns changed by -2.39 to target drift = 0.17 nm/sec Measured defocus = -2.19 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6417.tif 27 frames are being saved to U:\krios\20190717\PilA_6418.tif 27 frames are being saved to U:\krios\20190717\PilA_6419.tif 27 frames are being saved to U:\krios\20190717\PilA_6420.tif 27 frames are being saved to U:\krios\20190717\PilA_6421.tif 27 frames are being saved to U:\krios\20190717\PilA_6422.tif Running script 17 at item 199 (-224.02, 540.45) Remaining time to fill dewars is 12780 Dewars are NOT busy filling Trimming A by 21 67 90 20, reference by 220 21 20 215 Trimming A by 21 67 90 20, reference by 156 23 145 20 Trimming A by 21 67 90 20, reference by 20 126 20 175 Trimming A by 21 67 90 20, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.21 -0.14 Trimming A by 20 20 20 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: -0.02 -0.30 (2nd move error -0.24 -0.18) Finished aligning to item in 2 rounds -18.7 48.5 unbinned pixels; 202.8 5.9 nm along two shift axes; -184.8 -82.8 nm on specimen axes Total_Shift = 103.970803 1.1 33.7 unbinned pixels; 122.4 54.7 nm along two shift axes; -131.3 -2.7 nm on specimen axes Total_Shift = 67.413113 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.01 microns changed by -2.31 to target drift = 0.01 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6423.tif 27 frames are being saved to U:\krios\20190717\PilA_6424.tif 27 frames are being saved to U:\krios\20190717\PilA_6425.tif 27 frames are being saved to U:\krios\20190717\PilA_6426.tif 27 frames are being saved to U:\krios\20190717\PilA_6427.tif 27 frames are being saved to U:\krios\20190717\PilA_6428.tif Running script 17 at item 200 (-222.61, 537.72) Remaining time to fill dewars is 12540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.16 -0.28 (2nd move error -0.06 -0.16) Finished aligning to item in 1 rounds -17.2 11.2 unbinned pixels; 63.7 45.1 nm along two shift axes; -40.0 -69.3 nm on specimen axes Total_Shift = 41.060236 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.11 microns changed by -2.29 to target drift = 0.03 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6429.tif 27 frames are being saved to U:\krios\20190717\PilA_6430.tif 27 frames are being saved to U:\krios\20190717\PilA_6431.tif 27 frames are being saved to U:\krios\20190717\PilA_6432.tif 27 frames are being saved to U:\krios\20190717\PilA_6433.tif 27 frames are being saved to U:\krios\20190717\PilA_6434.tif Running script 17 at item 201 (-221.20, 534.95) Remaining time to fill dewars is 12360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 35 30 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.10 -0.22 (2nd move error -0.11 -0.10) Finished aligning to item in 1 rounds Trimming A by 21 35 30 20, reference by 21 35 30 20 -13.5 23.1 unbinned pixels; 102.6 13.9 nm along two shift axes; -87.1 -57.5 nm on specimen axes Total_Shift = 53.558755 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.20 microns changed by -2.30 to target drift = 0.02 nm/sec Measured defocus = -2.52 microns changed by 0.02 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6435.tif 27 frames are being saved to U:\krios\20190717\PilA_6436.tif 27 frames are being saved to U:\krios\20190717\PilA_6437.tif 27 frames are being saved to U:\krios\20190717\PilA_6438.tif 27 frames are being saved to U:\krios\20190717\PilA_6439.tif 27 frames are being saved to U:\krios\20190717\PilA_6440.tif Running script 17 at item 202 (-219.81, 532.23) Remaining time to fill dewars is 12180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 100 90 20, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.28 -0.22 (2nd move error 0.06 -0.10) Finished aligning to item in 1 rounds Trimming A by 22 100 90 20, reference by 22 100 90 20 -11.4 -3.2 unbinned pixels; 3.3 45.9 nm along two shift axes; 14.7 -43.9 nm on specimen axes Total_Shift = 23.741569 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.40 microns changed by -2.20 to target drift = 0.16 nm/sec Measured defocus = -2.58 microns changed by -0.02 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6441.tif 27 frames are being saved to U:\krios\20190717\PilA_6442.tif 27 frames are being saved to U:\krios\20190717\PilA_6443.tif 27 frames are being saved to U:\krios\20190717\PilA_6444.tif 27 frames are being saved to U:\krios\20190717\PilA_6445.tif 27 frames are being saved to U:\krios\20190717\PilA_6446.tif Running script 17 at item 203 (-221.02, 528.12) Remaining time to fill dewars is 11880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 170 105 30, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.30 -0.28 (2nd move error 0.09 -0.15) Finished aligning to item in 1 rounds Trimming A by 22 175 100 20, reference by 21 170 105 30 -16.2 -1.7 unbinned pixels; 15.0 60.8 nm along two shift axes; 9.9 -62.7 nm on specimen axes Total_Shift = 32.569171 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.56 microns changed by -2.46 to target drift = 0.14 nm/sec Measured defocus = -0.96 microns changed by 0.06 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6447.tif 27 frames are being saved to U:\krios\20190717\PilA_6448.tif 27 frames are being saved to U:\krios\20190717\PilA_6449.tif 27 frames are being saved to U:\krios\20190717\PilA_6450.tif 27 frames are being saved to U:\krios\20190717\PilA_6451.tif 27 frames are being saved to U:\krios\20190717\PilA_6452.tif Running script 17 at item 204 (-222.41, 530.76) Remaining time to fill dewars is 11760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 90 55 60, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.28 -0.32 (2nd move error 0.07 -0.19) Finished aligning to item in 1 rounds Trimming A by 21 95 55 60, reference by 21 90 55 60 -24.7 -2.4 unbinned pixels; 23.2 92.5 nm along two shift axes; 14.7 -95.5 nm on specimen axes Total_Shift = 49.602288 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.47 microns changed by -2.47 to target drift = 0.13 nm/sec Measured defocus = -1.02 microns changed by 0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6453.tif 27 frames are being saved to U:\krios\20190717\PilA_6454.tif 27 frames are being saved to U:\krios\20190717\PilA_6455.tif 27 frames are being saved to U:\krios\20190717\PilA_6456.tif 27 frames are being saved to U:\krios\20190717\PilA_6457.tif 27 frames are being saved to U:\krios\20190717\PilA_6458.tif Running script 17 at item 205 (-223.83, 533.53) Remaining time to fill dewars is 11580 Dewars are NOT busy filling Trimming A by 23 71 85 20, reference by 220 21 20 215 Trimming A by 23 71 85 20, reference by 156 23 145 20 Trimming A by 23 71 85 20, reference by 20 126 20 175 Trimming A by 23 71 85 20, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.24 -0.12 Trimming A by 21 45 20 45, reference by 24 80 80 20 Disparity in stage position after aligning to target position: 0.08 -0.27 (2nd move error -0.16 -0.17) Finished aligning to item in 2 rounds Trimming A by 21 45 20 45, reference by 21 45 20 45 -24.1 38.5 unbinned pixels; 172.9 28.9 nm along two shift axes; -144.5 -101.9 nm on specimen axes Total_Shift = 90.795555 Trimming A by 21 45 20 50, reference by 21 45 20 45 -3.2 27.5 unbinned pixels; 105.5 29.8 nm along two shift axes; -106.5 -18.4 nm on specimen axes Total_Shift = 55.468394 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.27 microns changed by -2.37 to target drift = 0.01 nm/sec Measured defocus = -1.10 microns changed by -0.00 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6459.tif 27 frames are being saved to U:\krios\20190717\PilA_6460.tif 27 frames are being saved to U:\krios\20190717\PilA_6461.tif 27 frames are being saved to U:\krios\20190717\PilA_6462.tif 27 frames are being saved to U:\krios\20190717\PilA_6463.tif 27 frames are being saved to U:\krios\20190717\PilA_6464.tif Running script 17 at item 206 (-225.30, 535.98) Remaining time to fill dewars is 11400 Dewars are NOT busy filling Trimming A by 23 130 20 180, reference by 220 21 20 215 Trimming A by 23 130 20 180, reference by 156 23 145 20 Trimming A by 23 130 20 180, reference by 20 126 20 175 Trimming A by 23 130 20 180, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.13 Trimming A by 20 20 20 30, reference by 20 126 20 175 Disparity in stage position after aligning to target position: 0.24 0.03 (2nd move error 0.10 0.14) Finished aligning to item in 2 rounds Trimming A by 20 20 20 25, reference by 20 20 20 30 1.2 5.6 unbinned pixels; 19.0 12.9 nm along two shift axes; -22.0 3.6 nm on specimen axes Total_Shift = 11.460271 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.29 microns changed by -2.49 to target drift = 0.11 nm/sec Measured defocus = -1.24 microns changed by 0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6465.tif 27 frames are being saved to U:\krios\20190717\PilA_6466.tif 27 frames are being saved to U:\krios\20190717\PilA_6467.tif 27 frames are being saved to U:\krios\20190717\PilA_6468.tif 27 frames are being saved to U:\krios\20190717\PilA_6469.tif 27 frames are being saved to U:\krios\20190717\PilA_6470.tif Running script 17 at item 207 (-226.69, 538.77) Remaining time to fill dewars is 11160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 126 20 175 Disparity in stage position after aligning to target position: 0.22 -0.11 (2nd move error 0.08 -0.00) Finished aligning to item in 1 rounds -1.5 -3.1 unbinned pixels; 9.5 10.3 nm along two shift axes; 12.5 -5.3 nm on specimen axes Total_Shift = 6.975477 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.13 microns changed by -2.43 to target drift = 0.14 nm/sec Measured defocus = -1.30 microns changed by 0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6471.tif 27 frames are being saved to U:\krios\20190717\PilA_6472.tif 27 frames are being saved to U:\krios\20190717\PilA_6473.tif 27 frames are being saved to U:\krios\20190717\PilA_6474.tif 27 frames are being saved to U:\krios\20190717\PilA_6475.tif 27 frames are being saved to U:\krios\20190717\PilA_6476.tif Running script 17 at item 208 (-228.30, 541.43) Remaining time to fill dewars is 10980 Dewars are NOT busy filling Trimming A by 217 21 20 235, reference by 220 21 20 215 Trimming A by 217 21 20 235, reference by 156 23 145 20 Trimming A by 217 21 20 235, reference by 20 126 20 175 Trimming A by 217 21 20 235, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.07 Trimming A by 75 20 20 80, reference by 220 21 20 215 Disparity in stage position after aligning to target position: 0.48 -0.03 (2nd move error 0.33 0.03) Finished aligning to item in 2 rounds Trimming A by 70 20 20 75, reference by 75 20 20 80 2.5 4.7 unbinned pixels; 14.0 16.0 nm along two shift axes; -18.8 8.6 nm on specimen axes Total_Shift = 10.604733 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.09 microns changed by -2.49 to target drift = 0.06 nm/sec Measured defocus = -1.53 microns changed by 0.13 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6477.tif 27 frames are being saved to U:\krios\20190717\PilA_6478.tif 27 frames are being saved to U:\krios\20190717\PilA_6479.tif 27 frames are being saved to U:\krios\20190717\PilA_6480.tif 27 frames are being saved to U:\krios\20190717\PilA_6481.tif 27 frames are being saved to U:\krios\20190717\PilA_6482.tif Running script 17 at item 209 (-230.77, 540.09) Remaining time to fill dewars is 10800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 62 20 20 135, reference by 20 126 20 175 Disparity in stage position after aligning to target position: 0.12 -0.13 (2nd move error -0.02 -0.02) Finished aligning to item in 1 rounds Trimming A by 62 20 20 135, reference by 62 20 20 135 -4.2 4.3 unbinned pixels; 21.3 8.8 nm along two shift axes; -15.8 -17.4 nm on specimen axes Total_Shift = 12.051653 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.04 microns changed by -2.54 to target drift = 0.08 nm/sec Measured defocus = -1.48 microns changed by -0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6483.tif 27 frames are being saved to U:\krios\20190717\PilA_6484.tif 27 frames are being saved to U:\krios\20190717\PilA_6485.tif 27 frames are being saved to U:\krios\20190717\PilA_6486.tif 27 frames are being saved to U:\krios\20190717\PilA_6487.tif 27 frames are being saved to U:\krios\20190717\PilA_6488.tif Running script 17 at item 210 (-229.28, 537.31) Remaining time to fill dewars is 10560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 37 20 95, reference by 20 126 20 175 Disparity in stage position after aligning to target position: 0.19 -0.10 (2nd move error 0.05 0.01) Finished aligning to item in 1 rounds Trimming A by 20 41 20 90, reference by 22 37 20 95 0.8 -3.8 unbinned pixels; 14.9 2.8 nm along two shift axes; 14.5 3.9 nm on specimen axes Total_Shift = 7.702898 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.93 microns changed by -2.53 to target drift = 0.13 nm/sec Measured defocus = -1.64 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6489.tif 27 frames are being saved to U:\krios\20190717\PilA_6490.tif 27 frames are being saved to U:\krios\20190717\PilA_6491.tif 27 frames are being saved to U:\krios\20190717\PilA_6492.tif 27 frames are being saved to U:\krios\20190717\PilA_6493.tif 27 frames are being saved to U:\krios\20190717\PilA_6494.tif Running script 17 at item 211 (-227.88, 534.55) Remaining time to fill dewars is 10380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 75 20 95, reference by 20 126 20 175 Disparity in stage position after aligning to target position: 0.29 -0.09 (2nd move error 0.15 0.02) Finished aligning to item in 1 rounds Trimming A by 21 75 20 90, reference by 21 75 20 95 2.7 1.8 unbinned pixels; 3.1 12.3 nm along two shift axes; -7.5 10.0 nm on specimen axes Total_Shift = 6.405441 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.77 microns changed by -2.47 to target drift = 0.12 nm/sec Measured defocus = -1.73 microns changed by 0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6495.tif 27 frames are being saved to U:\krios\20190717\PilA_6496.tif 27 frames are being saved to U:\krios\20190717\PilA_6497.tif 27 frames are being saved to U:\krios\20190717\PilA_6498.tif 27 frames are being saved to U:\krios\20190717\PilA_6499.tif 27 frames are being saved to U:\krios\20190717\PilA_6500.tif Running script 17 at item 212 (-226.55, 531.83) Remaining time to fill dewars is 10140 Dewars are NOT busy filling Trimming A by 22 127 20 185, reference by 220 21 20 215 Trimming A by 22 127 20 185, reference by 156 23 145 20 Trimming A by 22 127 20 185, reference by 20 126 20 175 Trimming A by 22 127 20 185, reference by 24 80 80 20 Disparity in stage position after aligning to center of map frame: 0.21 -0.14 Trimming A by 24 110 20 120, reference by 20 126 20 175 Disparity in stage position after aligning to target position: 0.42 -0.03 (2nd move error 0.20 0.10) Finished aligning to item in 2 rounds Trimming A by 23 110 20 115, reference by 24 110 20 120 0.3 -4.6 unbinned pixels; 17.2 5.8 nm along two shift axes; 17.7 2.2 nm on specimen axes Total_Shift = 9.166999 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.68 microns changed by -2.48 to target drift = 0.16 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6501.tif 27 frames are being saved to U:\krios\20190717\PilA_6502.tif 27 frames are being saved to U:\krios\20190717\PilA_6503.tif 27 frames are being saved to U:\krios\20190717\PilA_6504.tif 27 frames are being saved to U:\krios\20190717\PilA_6505.tif 27 frames are being saved to U:\krios\20190717\PilA_6506.tif Running script 17 at item 213 (-225.16, 529.10) Remaining time to fill dewars is 9960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 155 20 130, reference by 20 126 20 175 Disparity in stage position after aligning to target position: 0.41 -0.01 (2nd move error 0.19 0.11) Finished aligning to item in 1 rounds Trimming A by 20 150 20 125, reference by 24 155 20 130 1.0 10.2 unbinned pixels; 36.2 18.8 nm along two shift axes; -39.8 1.6 nm on specimen axes Total_Shift = 20.442935 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.54 microns changed by -2.44 to target drift = 0.15 nm/sec Measured defocus = -1.93 microns changed by 0.03 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6507.tif 27 frames are being saved to U:\krios\20190717\PilA_6508.tif 27 frames are being saved to U:\krios\20190717\PilA_6509.tif 27 frames are being saved to U:\krios\20190717\PilA_6510.tif 27 frames are being saved to U:\krios\20190717\PilA_6511.tif 27 frames are being saved to U:\krios\20190717\PilA_6512.tif Running script 17 at item 227-1 (62.49, 282.95) Remaining time to fill dewars is 9840 Dewars are NOT busy filling Trimming A by 22 140 130 20, reference by 225 21 20 215 Trimming A by 22 140 130 20, reference by 200 24 160 20 Trimming A by 22 140 130 20, reference by 21 150 20 190 Trimming A by 22 140 130 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.19 Trimming A by 20 81 155 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.01 -0.09 (2nd move error -0.00 0.08) Finished aligning to item in 2 rounds Trimming A by 24 72 160 20, reference by 20 81 155 20 -0.9 2.3 unbinned pixels; 9.9 0.2 nm along two shift axes; -8.9 -4.1 nm on specimen axes Total_Shift = 5.056792 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.86 microns changed by -2.86 to target drift = 0.12 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6513.tif 27 frames are being saved to U:\krios\20190717\PilA_6514.tif 27 frames are being saved to U:\krios\20190717\PilA_6515.tif 27 frames are being saved to U:\krios\20190717\PilA_6516.tif 27 frames are being saved to U:\krios\20190717\PilA_6517.tif 27 frames are being saved to U:\krios\20190717\PilA_6518.tif Running script 17 at item 228-1 (60.95, 285.67) Remaining time to fill dewars is 9660 Dewars are NOT busy filling Trimming A by 205 21 165 20, reference by 225 21 20 215 Trimming A by 205 21 165 20, reference by 200 24 160 20 Trimming A by 205 21 165 20, reference by 21 150 20 190 Trimming A by 205 21 165 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.00 -0.14 Trimming A by 95 20 120 20, reference by 200 24 160 20 Disparity in stage position after aligning to target position: 0.04 -0.11 (2nd move error 0.04 0.02) Finished aligning to item in 2 rounds Trimming A by 90 22 125 20, reference by 95 20 120 20 1.7 -2.0 unbinned pixels; 9.7 3.0 nm along two shift axes; 7.5 7.0 nm on specimen axes Total_Shift = 5.278774 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.70 microns changed by -2.80 to target drift = 0.14 nm/sec Measured defocus = -2.10 microns changed by 0.00 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6519.tif 27 frames are being saved to U:\krios\20190717\PilA_6520.tif 27 frames are being saved to U:\krios\20190717\PilA_6521.tif 27 frames are being saved to U:\krios\20190717\PilA_6522.tif 27 frames are being saved to U:\krios\20190717\PilA_6523.tif 27 frames are being saved to U:\krios\20190717\PilA_6524.tif Running script 17 at item 229-1 (55.47, 289.71) Remaining time to fill dewars is 9360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 105 21 70 20, reference by 200 24 160 20 Disparity in stage position after aligning to target position: 0.05 -0.21 (2nd move error 0.04 -0.09) Finished aligning to item in 1 rounds Trimming A by 105 21 70 20, reference by 105 21 70 20 -12.1 -1.5 unbinned pixels; 10.2 45.8 nm along two shift axes; 8.4 -46.7 nm on specimen axes Total_Shift = 24.371588 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.83 microns changed by -1.37 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6525.tif 27 frames are being saved to U:\krios\20190717\PilA_6526.tif 27 frames are being saved to U:\krios\20190717\PilA_6527.tif 27 frames are being saved to U:\krios\20190717\PilA_6528.tif 27 frames are being saved to U:\krios\20190717\PilA_6529.tif 27 frames are being saved to U:\krios\20190717\PilA_6530.tif Running script 17 at item 230-1 (56.86, 286.99) Remaining time to fill dewars is 9240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 51 21 70 20, reference by 200 24 160 20 Disparity in stage position after aligning to target position: 0.02 -0.16 (2nd move error 0.01 -0.05) Finished aligning to item in 1 rounds Trimming A by 51 21 70 20, reference by 51 21 70 20 -7.8 -1.0 unbinned pixels; 6.4 29.4 nm along two shift axes; 5.5 -30.0 nm on specimen axes Total_Shift = 15.644816 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.48 microns changed by -2.78 to target drift = 0.09 nm/sec Measured defocus = -2.32 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6531.tif 27 frames are being saved to U:\krios\20190717\PilA_6532.tif 27 frames are being saved to U:\krios\20190717\PilA_6533.tif 27 frames are being saved to U:\krios\20190717\PilA_6534.tif 27 frames are being saved to U:\krios\20190717\PilA_6535.tif 27 frames are being saved to U:\krios\20190717\PilA_6536.tif Running script 17 at item 231-1 (58.45, 284.30) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 30 22 50 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: -0.01 -0.19 (2nd move error -0.02 -0.02) Finished aligning to item in 1 rounds Trimming A by 30 22 50 20, reference by 30 22 50 20 -3.9 5.1 unbinned pixels; 23.8 6.3 nm along two shift axes; -19.0 -16.1 nm on specimen axes Total_Shift = 12.789226 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.85 microns changed by -1.55 to target drift = 0.03 nm/sec Measured defocus = -2.42 microns changed by 0.02 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6537.tif 27 frames are being saved to U:\krios\20190717\PilA_6538.tif 27 frames are being saved to U:\krios\20190717\PilA_6539.tif 27 frames are being saved to U:\krios\20190717\PilA_6540.tif 27 frames are being saved to U:\krios\20190717\PilA_6541.tif 27 frames are being saved to U:\krios\20190717\PilA_6542.tif Running script 17 at item 232-1 (59.83, 281.52) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 90 95 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.02 -0.18 (2nd move error 0.01 -0.01) Finished aligning to item in 1 rounds Trimming A by 23 90 95 20, reference by 23 90 95 20 -3.8 0.1 unbinned pixels; 5.4 13.5 nm along two shift axes; 0.4 -14.8 nm on specimen axes Total_Shift = 7.615349 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.33 microns changed by -2.83 to target drift = 0.09 nm/sec Measured defocus = -2.44 microns changed by -0.06 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6543.tif 27 frames are being saved to U:\krios\20190717\PilA_6544.tif 27 frames are being saved to U:\krios\20190717\PilA_6545.tif 27 frames are being saved to U:\krios\20190717\PilA_6546.tif 27 frames are being saved to U:\krios\20190717\PilA_6547.tif 27 frames are being saved to U:\krios\20190717\PilA_6548.tif Running script 17 at item 233-1 (61.27, 278.78) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 22 140 130 20, reference by 225 21 20 215 Trimming A by 22 140 130 20, reference by 200 24 160 20 Trimming A by 22 140 130 20, reference by 21 150 20 190 Trimming A by 22 140 130 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.17 Trimming A by 20 155 160 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.04 -0.05 (2nd move error -0.02 0.10) Finished aligning to item in 2 rounds Trimming A by 23 142 170 20, reference by 20 155 160 20 -2.1 6.7 unbinned pixels; 27.3 2.5 nm along two shift axes; -25.5 -9.6 nm on specimen axes Total_Shift = 14.001399 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -1.08 microns changed by -1.52 to target drift = 0.12 nm/sec Measured defocus = -2.54 microns changed by -0.06 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6549.tif 27 frames are being saved to U:\krios\20190717\PilA_6550.tif 27 frames are being saved to U:\krios\20190717\PilA_6551.tif 27 frames are being saved to U:\krios\20190717\PilA_6552.tif 27 frames are being saved to U:\krios\20190717\PilA_6553.tif 27 frames are being saved to U:\krios\20190717\PilA_6554.tif Running script 17 at item 234 (57.19, 280.08) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 75 65 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: -0.02 -0.22 (2nd move error -0.09 -0.07) Finished aligning to item in 1 rounds Trimming A by 24 75 65 20, reference by 24 75 65 20 -7.2 17.7 unbinned pixels; 74.5 0.7 nm along two shift axes; -67.3 -31.8 nm on specimen axes Total_Shift = 38.207785 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.14 microns changed by -3.04 to target drift = 0.09 nm/sec Measured defocus = -0.98 microns changed by 0.08 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6555.tif 27 frames are being saved to U:\krios\20190717\PilA_6556.tif 27 frames are being saved to U:\krios\20190717\PilA_6557.tif 27 frames are being saved to U:\krios\20190717\PilA_6558.tif 27 frames are being saved to U:\krios\20190717\PilA_6559.tif 27 frames are being saved to U:\krios\20190717\PilA_6560.tif Running script 17 at item 235 (55.81, 282.90) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.01 -0.21 (2nd move error -0.06 -0.06) Finished aligning to item in 1 rounds -7.4 11.5 unbinned pixels; 51.7 9.3 nm along two shift axes; -43.0 -31.1 nm on specimen axes Total_Shift = 27.236341 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.01 microns changed by -3.01 to target drift = 0.12 nm/sec Measured defocus = -1.03 microns changed by 0.03 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6561.tif 27 frames are being saved to U:\krios\20190717\PilA_6562.tif 27 frames are being saved to U:\krios\20190717\PilA_6563.tif 27 frames are being saved to U:\krios\20190717\PilA_6564.tif 27 frames are being saved to U:\krios\20190717\PilA_6565.tif 27 frames are being saved to U:\krios\20190717\PilA_6566.tif Running script 17 at item 236 (54.26, 285.64) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 200 24 160 20, reference by 225 21 20 215 Trimming A by 200 24 160 20, reference by 200 24 160 20 Trimming A by 200 24 160 20, reference by 21 150 20 190 Trimming A by 200 24 160 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.06 Trimming A by 20 20 20 20, reference by 200 24 160 20 Disparity in stage position after aligning to target position: 0.11 -0.26 (2nd move error 0.02 -0.22) Finished aligning to item in 2 rounds -21.8 -1.4 unbinned pixels; 23.5 80.7 nm along two shift axes; 10.0 -84.7 nm on specimen axes Total_Shift = 43.762823 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.76 microns changed by -2.86 to target drift = 0.12 nm/sec Measured defocus = -1.10 microns changed by 0.00 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6567.tif 27 frames are being saved to U:\krios\20190717\PilA_6568.tif 27 frames are being saved to U:\krios\20190717\PilA_6569.tif 27 frames are being saved to U:\krios\20190717\PilA_6570.tif 27 frames are being saved to U:\krios\20190717\PilA_6571.tif 27 frames are being saved to U:\krios\20190717\PilA_6572.tif Running script 17 at item 237 (52.83, 288.00) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 230 23 20 220, reference by 225 21 20 215 Trimming A by 230 23 20 220, reference by 200 24 160 20 Trimming A by 230 23 20 220, reference by 21 150 20 190 Trimming A by 230 23 20 220, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.01 Trimming A by 70 20 20 65, reference by 225 21 20 215 Disparity in stage position after aligning to target position: 0.25 0.16 (2nd move error 0.18 0.15) Finished aligning to item in 2 rounds Trimming A by 70 20 20 65, reference by 70 20 20 65 3.5 1.4 unbinned pixels; 0.6 14.6 nm along two shift axes; -6.1 13.2 nm on specimen axes Total_Shift = 7.490976 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.62 microns changed by -2.82 to target drift = 0.19 nm/sec Measured defocus = -1.21 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6573.tif 27 frames are being saved to U:\krios\20190717\PilA_6574.tif 27 frames are being saved to U:\krios\20190717\PilA_6575.tif 27 frames are being saved to U:\krios\20190717\PilA_6576.tif 27 frames are being saved to U:\krios\20190717\PilA_6577.tif 27 frames are being saved to U:\krios\20190717\PilA_6578.tif Running script 17 at item 238 (51.41, 290.72) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 24 20 115, reference by 225 21 20 215 Disparity in stage position after aligning to target position: 0.26 0.06 (2nd move error 0.19 0.05) Finished aligning to item in 1 rounds Trimming A by 150 21 75 110, reference by 150 24 20 115 1.4 6.5 unbinned pixels; 21.9 14.7 nm along two shift axes; -25.4 4.1 nm on specimen axes Total_Shift = 13.207757 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.51 microns changed by -2.81 to target drift = 0.15 nm/sec Measured defocus = -1.30 microns changed by -0.00 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6579.tif 27 frames are being saved to U:\krios\20190717\PilA_6580.tif 27 frames are being saved to U:\krios\20190717\PilA_6581.tif 27 frames are being saved to U:\krios\20190717\PilA_6582.tif 27 frames are being saved to U:\krios\20190717\PilA_6583.tif 27 frames are being saved to U:\krios\20190717\PilA_6584.tif Running script 17 at item 239 (50.09, 286.62) Remaining time to fill dewars is 8880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 85 24 20 95, reference by 225 21 20 215 Disparity in stage position after aligning to target position: 0.30 0.03 (2nd move error 0.24 0.02) Finished aligning to item in 1 rounds Trimming A by 85 24 20 90, reference by 85 24 20 95 2.1 -9.2 unbinned pixels; 36.8 6.2 nm along two shift axes; 35.5 10.3 nm on specimen axes Total_Shift = 18.985053 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.50 microns changed by -2.90 to target drift = 0.13 nm/sec Measured defocus = -1.44 microns changed by 0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6585.tif 27 frames are being saved to U:\krios\20190717\PilA_6586.tif 27 frames are being saved to U:\krios\20190717\PilA_6587.tif 27 frames are being saved to U:\krios\20190717\PilA_6588.tif 27 frames are being saved to U:\krios\20190717\PilA_6589.tif 27 frames are being saved to U:\krios\20190717\PilA_6590.tif Running script 17 at item 240 (51.71, 283.83) Remaining time to fill dewars is 7680 Dewars are NOT busy filling Trimming A by 22 150 20 195, reference by 225 21 20 215 Trimming A by 22 150 20 195, reference by 200 24 160 20 Trimming A by 22 150 20 195, reference by 21 150 20 190 Trimming A by 22 150 20 195, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.09 Trimming A by 20 20 20 20, reference by 21 150 20 190 Disparity in stage position after aligning to target position: 0.09 0.08 (2nd move error -0.03 0.15) Finished aligning to item in 2 rounds 4.1 7.5 unbinned pixels; 22.1 26.1 nm along two shift axes; -29.9 14.5 nm on specimen axes Total_Shift = 17.053024 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.40 microns changed by -2.90 to target drift = 0.18 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6591.tif 27 frames are being saved to U:\krios\20190717\PilA_6592.tif 27 frames are being saved to U:\krios\20190717\PilA_6593.tif 27 frames are being saved to U:\krios\20190717\PilA_6594.tif 27 frames are being saved to U:\krios\20190717\PilA_6595.tif 27 frames are being saved to U:\krios\20190717\PilA_6596.tif Running script 17 at item 241 (53.10, 281.10) Remaining time to fill dewars is 7500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 30 20 35, reference by 21 150 20 190 Disparity in stage position after aligning to target position: 0.14 0.08 (2nd move error 0.01 0.15) Finished aligning to item in 1 rounds Trimming A by 20 30 20 30, reference by 20 30 20 35 -2.6 0.5 unbinned pixels; 5.4 8.7 nm along two shift axes; -1.5 -10.3 nm on specimen axes Total_Shift = 5.363601 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.34 microns changed by -2.94 to target drift = 0.12 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6597.tif 27 frames are being saved to U:\krios\20190717\PilA_6598.tif 27 frames are being saved to U:\krios\20190717\PilA_6599.tif 27 frames are being saved to U:\krios\20190717\PilA_6600.tif 27 frames are being saved to U:\krios\20190717\PilA_6601.tif 27 frames are being saved to U:\krios\20190717\PilA_6602.tif Running script 17 at item 242 (54.63, 278.68) Remaining time to fill dewars is 7380 Dewars are NOT busy filling Trimming A by 24 140 130 20, reference by 225 21 20 215 Trimming A by 24 140 130 20, reference by 200 24 160 20 Trimming A by 24 140 130 20, reference by 21 150 20 190 Trimming A by 24 140 130 20, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.07 -0.09 Trimming A by 21 60 35 55, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.09 -0.25 (2nd move error 0.01 -0.19) Finished aligning to item in 2 rounds Trimming A by 21 65 30 50, reference by 21 60 35 55 -24.0 -0.2 unbinned pixels; 30.5 86.6 nm along two shift axes; 5.9 -93.2 nm on specimen axes Total_Shift = 47.923936 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.35 microns changed by -3.05 to target drift = 0.05 nm/sec Measured defocus = -1.76 microns changed by 0.06 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6603.tif 27 frames are being saved to U:\krios\20190717\PilA_6604.tif 27 frames are being saved to U:\krios\20190717\PilA_6605.tif 27 frames are being saved to U:\krios\20190717\PilA_6606.tif 27 frames are being saved to U:\krios\20190717\PilA_6607.tif 27 frames are being saved to U:\krios\20190717\PilA_6608.tif Running script 17 at item 243 (55.95, 275.95) Remaining time to fill dewars is 7140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 135 80 35, reference by 22 145 130 20 Disparity in stage position after aligning to target position: 0.14 -0.20 (2nd move error 0.06 -0.13) Finished aligning to item in 1 rounds Trimming A by 22 135 85 35, reference by 22 135 80 35 -16.2 1.0 unbinned pixels; 24.7 56.8 nm along two shift axes; -0.3 -63.2 nm on specimen axes Total_Shift = 32.425121 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.24 microns changed by -3.04 to target drift = 0.09 nm/sec Measured defocus = -1.82 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6609.tif 27 frames are being saved to U:\krios\20190717\PilA_6610.tif 27 frames are being saved to U:\krios\20190717\PilA_6611.tif 27 frames are being saved to U:\krios\20190717\PilA_6612.tif 27 frames are being saved to U:\krios\20190717\PilA_6613.tif 27 frames are being saved to U:\krios\20190717\PilA_6614.tif Running script 17 at item 244 (53.16, 274.23) Remaining time to fill dewars is 6960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 180 20 125, reference by 21 150 20 190 Disparity in stage position after aligning to target position: 0.25 0.07 (2nd move error 0.13 0.13) Finished aligning to item in 1 rounds Trimming A by 20 180 20 120, reference by 20 180 20 125 1.7 -1.2 unbinned pixels; 6.4 4.2 nm along two shift axes; 4.1 6.7 nm on specimen axes Total_Shift = 4.02731 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.07 microns changed by -2.97 to target drift = 0.13 nm/sec Measured defocus = -1.85 microns changed by -0.05 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6615.tif 27 frames are being saved to U:\krios\20190717\PilA_6616.tif 27 frames are being saved to U:\krios\20190717\PilA_6617.tif 27 frames are being saved to U:\krios\20190717\PilA_6618.tif 27 frames are being saved to U:\krios\20190717\PilA_6619.tif 27 frames are being saved to U:\krios\20190717\PilA_6620.tif Running script 17 at item 245 (50.50, 279.70) Remaining time to fill dewars is 6780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 85 20 100, reference by 21 150 20 190 Disparity in stage position after aligning to target position: 0.22 0.01 (2nd move error 0.09 0.08) Finished aligning to item in 1 rounds Trimming A by 20 85 20 95, reference by 20 85 20 100 -0.7 -2.9 unbinned pixels; 9.6 7.0 nm along two shift axes; 11.3 -2.3 nm on specimen axes Total_Shift = 5.924099 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = -0.36 microns changed by -1.64 to target drift = 0.13 nm/sec Measured defocus = -1.96 microns changed by -0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6621.tif 27 frames are being saved to U:\krios\20190717\PilA_6622.tif 27 frames are being saved to U:\krios\20190717\PilA_6623.tif 27 frames are being saved to U:\krios\20190717\PilA_6624.tif 27 frames are being saved to U:\krios\20190717\PilA_6625.tif 27 frames are being saved to U:\krios\20190717\PilA_6626.tif Running script 17 at item 246 (49.11, 282.47) Remaining time to fill dewars is 6660 Dewars are NOT busy filling Trimming A by 22 150 20 195, reference by 225 21 20 215 Trimming A by 22 150 20 195, reference by 200 24 160 20 Trimming A by 22 150 20 195, reference by 21 150 20 190 Trimming A by 22 150 20 195, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.09 Trimming A by 21 42 20 100, reference by 21 150 20 190 Disparity in stage position after aligning to target position: 0.08 0.01 (2nd move error -0.07 0.07) Finished aligning to item in 2 rounds Trimming A by 23 33 20 105, reference by 21 42 20 100 1.1 14.6 unbinned pixels; 52.2 25.9 nm along two shift axes; -57.0 1.2 nm on specimen axes Total_Shift = 29.249773 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.83 microns changed by -2.93 to target drift = 0.16 nm/sec Measured defocus = -2.09 microns changed by -0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6627.tif 27 frames are being saved to U:\krios\20190717\PilA_6628.tif 27 frames are being saved to U:\krios\20190717\PilA_6629.tif 27 frames are being saved to U:\krios\20190717\PilA_6630.tif 27 frames are being saved to U:\krios\20190717\PilA_6631.tif 27 frames are being saved to U:\krios\20190717\PilA_6632.tif Running script 17 at item 247 (47.63, 285.17) Remaining time to fill dewars is 6420 Dewars are NOT busy filling Trimming A by 230 23 20 220, reference by 225 21 20 215 Trimming A by 230 23 20 220, reference by 200 24 160 20 Trimming A by 230 23 20 220, reference by 21 150 20 190 Trimming A by 230 23 20 220, reference by 22 145 130 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.04 Trimming A by 81 21 20 140, reference by 225 21 20 215 Disparity in stage position after aligning to target position: 0.40 0.01 (2nd move error 0.22 0.02) Finished aligning to item in 2 rounds Trimming A by 72 21 20 140, reference by 81 21 20 140 1.6 -1.1 unbinned pixels; 6.1 4.0 nm along two shift axes; 4.0 6.3 nm on specimen axes Total_Shift = 3.834461 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.67 microns changed by -1.53 to target drift = 0.13 nm/sec Measured defocus = -2.15 microns changed by -0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6633.tif 27 frames are being saved to U:\krios\20190717\PilA_6634.tif 27 frames are being saved to U:\krios\20190717\PilA_6635.tif 27 frames are being saved to U:\krios\20190717\PilA_6636.tif 27 frames are being saved to U:\krios\20190717\PilA_6637.tif 27 frames are being saved to U:\krios\20190717\PilA_6638.tif Running script 17 at item 248 (46.49, 281.06) Remaining time to fill dewars is 6240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 92 20 175, reference by 21 150 20 190 Disparity in stage position after aligning to target position: 0.12 -0.12 (2nd move error -0.03 -0.05) Finished aligning to item in 1 rounds Trimming A by 23 92 20 175, reference by 23 92 20 175 -6.1 6.8 unbinned pixels; 33.1 11.5 nm along two shift axes; -25.3 -25.0 nm on specimen axes Total_Shift = 18.282295 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.64 microns changed by -2.94 to target drift = 0.06 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6639.tif 27 frames are being saved to U:\krios\20190717\PilA_6640.tif 27 frames are being saved to U:\krios\20190717\PilA_6641.tif 27 frames are being saved to U:\krios\20190717\PilA_6642.tif 27 frames are being saved to U:\krios\20190717\PilA_6643.tif 27 frames are being saved to U:\krios\20190717\PilA_6644.tif Running script 17 at item 249 (47.92, 278.25) Remaining time to fill dewars is 6060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 127 20 185, reference by 21 150 20 190 Disparity in stage position after aligning to target position: 0.26 -0.04 (2nd move error 0.12 0.02) Finished aligning to item in 1 rounds Trimming A by 22 135 20 175, reference by 24 127 20 185 1.9 2.5 unbinned pixels; 6.8 10.6 nm along two shift axes; -10.2 6.8 nm on specimen axes Total_Shift = 6.283482 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.72 microns changed by -1.68 to target drift = 0.13 nm/sec Measured defocus = -2.41 microns changed by 0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6645.tif 27 frames are being saved to U:\krios\20190717\PilA_6646.tif 27 frames are being saved to U:\krios\20190717\PilA_6647.tif 27 frames are being saved to U:\krios\20190717\PilA_6648.tif 27 frames are being saved to U:\krios\20190717\PilA_6649.tif 27 frames are being saved to U:\krios\20190717\PilA_6650.tif Running script 17 at item 250 (68.01, 194.99) Remaining time to fill dewars is 5820 Dewars are NOT busy filling Trimming A by 23 155 145 20, reference by 211 20 20 210 Trimming A by 23 155 145 20, reference by 205 24 170 20 Trimming A by 23 155 145 20, reference by 24 151 20 190 Trimming A by 23 155 145 20, reference by 23 155 150 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.15 Trimming A by 70 21 215 20, reference by 23 155 150 20 Disparity in stage position after aligning to target position: -0.09 -0.03 (2nd move error -0.13 0.10) Finished aligning to item in 2 rounds Trimming A by 70 21 215 20, reference by 70 21 215 20 3.0 23.4 unbinned pixels; 82.0 45.8 nm along two shift axes; -91.5 6.6 nm on specimen axes Total_Shift = 47.100889 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.49 microns changed by -2.99 to target drift = 0.19 nm/sec Measured defocus = -2.55 microns changed by 0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6651.tif 27 frames are being saved to U:\krios\20190717\PilA_6652.tif 27 frames are being saved to U:\krios\20190717\PilA_6653.tif 27 frames are being saved to U:\krios\20190717\PilA_6654.tif 27 frames are being saved to U:\krios\20190717\PilA_6655.tif 27 frames are being saved to U:\krios\20190717\PilA_6656.tif Running script 17 at item 251 (66.32, 197.76) Remaining time to fill dewars is 5700 Dewars are NOT busy filling Trimming A by 192 22 190 20, reference by 211 20 20 210 Trimming A by 192 22 190 20, reference by 205 24 170 20 Trimming A by 192 22 190 20, reference by 24 151 20 190 Trimming A by 192 22 190 20, reference by 23 155 150 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.16 Trimming A by 105 23 185 20, reference by 205 24 170 20 Disparity in stage position after aligning to target position: 0.18 -0.10 (2nd move error 0.13 0.03) Finished aligning to item in 2 rounds Trimming A by 105 22 190 20, reference by 105 23 185 20 1.0 2.0 unbinned pixels; 5.9 6.7 nm along two shift axes; -7.9 3.6 nm on specimen axes Total_Shift = 4.44954 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -1.05 microns changed by -1.55 to target drift = 0.14 nm/sec Measured defocus = -2.57 microns changed by -0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6657.tif 27 frames are being saved to U:\krios\20190717\PilA_6658.tif 27 frames are being saved to U:\krios\20190717\PilA_6659.tif 27 frames are being saved to U:\krios\20190717\PilA_6660.tif 27 frames are being saved to U:\krios\20190717\PilA_6661.tif 27 frames are being saved to U:\krios\20190717\PilA_6662.tif Running script 17 at item 252 (60.91, 201.90) Remaining time to fill dewars is 5460 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 170 20 90 20, reference by 205 24 170 20 Disparity in stage position after aligning to target position: 0.03 -0.23 (2nd move error -0.02 -0.09) Finished aligning to item in 1 rounds Trimming A by 170 20 95 20, reference by 170 20 90 20 -4.5 40.3 unbinned pixels; 154.0 44.4 nm along two shift axes; -155.7 -26.0 nm on specimen axes Total_Shift = 81.051946 Trimming A by 160 20 100 20, reference by 170 20 90 20 1.0 29.5 unbinned pixels; 107.3 48.1 nm along two shift axes; -115.1 -2.3 nm on specimen axes Total_Shift = 59.094699 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.03 microns changed by -2.93 to target drift = 0.11 nm/sec Measured defocus = -0.94 microns changed by 0.04 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6663.tif 27 frames are being saved to U:\krios\20190717\PilA_6664.tif 27 frames are being saved to U:\krios\20190717\PilA_6665.tif 27 frames are being saved to U:\krios\20190717\PilA_6666.tif 27 frames are being saved to U:\krios\20190717\PilA_6667.tif 27 frames are being saved to U:\krios\20190717\PilA_6668.tif Running script 17 at item 253 (62.30, 199.08) Remaining time to fill dewars is 5220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 101 22 100 20, reference by 205 24 170 20 Disparity in stage position after aligning to target position: 0.23 -0.17 (2nd move error 0.18 -0.04) Finished aligning to item in 1 rounds Trimming A by 110 23 95 20, reference by 101 22 100 20 -3.9 -4.8 unbinned pixels; 12.4 21.2 nm along two shift axes; 19.3 -14.1 nm on specimen axes Total_Shift = 12.29612 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.93 microns changed by -2.93 to target drift = 0.09 nm/sec Measured defocus = -0.99 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6669.tif 27 frames are being saved to U:\krios\20190717\PilA_6670.tif 27 frames are being saved to U:\krios\20190717\PilA_6671.tif 27 frames are being saved to U:\krios\20190717\PilA_6672.tif 27 frames are being saved to U:\krios\20190717\PilA_6673.tif 27 frames are being saved to U:\krios\20190717\PilA_6674.tif Running script 17 at item 254 (63.71, 196.39) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 24 95 20, reference by 205 24 170 20 Disparity in stage position after aligning to target position: 0.20 -0.15 (2nd move error 0.15 -0.02) Finished aligning to item in 1 rounds Trimming A by 56 24 105 20, reference by 65 24 95 20 -0.5 2.6 unbinned pixels; 10.1 2.2 nm along two shift axes; -10.0 -2.4 nm on specimen axes Total_Shift = 5.253601 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.89 microns changed by -2.99 to target drift = 0.07 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6675.tif 27 frames are being saved to U:\krios\20190717\PilA_6676.tif 27 frames are being saved to U:\krios\20190717\PilA_6677.tif 27 frames are being saved to U:\krios\20190717\PilA_6678.tif 27 frames are being saved to U:\krios\20190717\PilA_6679.tif 27 frames are being saved to U:\krios\20190717\PilA_6680.tif Running script 17 at item 255 (65.34, 193.61) Remaining time to fill dewars is 4920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 45 45 115 20, reference by 23 155 150 20 Disparity in stage position after aligning to target position: 0.05 -0.12 (2nd move error 0.02 0.00) Finished aligning to item in 1 rounds Trimming A by 45 45 115 20, reference by 45 45 115 20 -0.2 -1.4 unbinned pixels; 4.8 2.7 nm along two shift axes; 5.4 -0.4 nm on specimen axes Total_Shift = 2.766948 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.82 microns changed by -3.02 to target drift = 0.12 nm/sec Measured defocus = -1.19 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6681.tif 27 frames are being saved to U:\krios\20190717\PilA_6682.tif 27 frames are being saved to U:\krios\20190717\PilA_6683.tif 27 frames are being saved to U:\krios\20190717\PilA_6684.tif 27 frames are being saved to U:\krios\20190717\PilA_6685.tif 27 frames are being saved to U:\krios\20190717\PilA_6686.tif Running script 17 at item 256 (66.69, 190.85) Remaining time to fill dewars is 4680 Dewars are NOT busy filling Trimming A by 23 155 145 20, reference by 211 20 20 210 Trimming A by 23 155 145 20, reference by 205 24 170 20 Trimming A by 23 155 145 20, reference by 24 151 20 190 Trimming A by 23 155 145 20, reference by 23 155 150 20 Disparity in stage position after aligning to center of map frame: 0.11 -0.15 Trimming A by 21 117 170 20, reference by 23 155 150 20 Disparity in stage position after aligning to target position: 0.06 -0.06 (2nd move error -0.05 0.07) Finished aligning to item in 2 rounds Trimming A by 23 130 155 20, reference by 21 117 170 20 -3.3 10.6 unbinned pixels; 43.2 3.9 nm along two shift axes; -40.4 -15.2 nm on specimen axes Total_Shift = 22.153096 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.81 microns changed by -3.11 to target drift = 0.11 nm/sec Measured defocus = -1.29 microns changed by -0.01 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6687.tif 27 frames are being saved to U:\krios\20190717\PilA_6688.tif 27 frames are being saved to U:\krios\20190717\PilA_6689.tif 27 frames are being saved to U:\krios\20190717\PilA_6690.tif 27 frames are being saved to U:\krios\20190717\PilA_6691.tif 27 frames are being saved to U:\krios\20190717\PilA_6692.tif Running script 17 at item 257 (64.05, 189.46) Remaining time to fill dewars is 4560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 120 115 20, reference by 23 155 150 20 Disparity in stage position after aligning to target position: 0.03 -0.18 (2nd move error -0.09 -0.05) Finished aligning to item in 1 rounds Trimming A by 20 120 110 20, reference by 20 120 115 20 -5.3 17.8 unbinned pixels; 72.2 7.6 nm along two shift axes; -68.0 -24.4 nm on specimen axes Total_Shift = 37.068395 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.71 microns changed by -3.11 to target drift = 0.09 nm/sec Measured defocus = -1.47 microns changed by 0.07 to target drift = 0.00 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6693.tif 27 frames are being saved to U:\krios\20190717\PilA_6694.tif 27 frames are being saved to U:\krios\20190717\PilA_6695.tif 27 frames are being saved to U:\krios\20190717\PilA_6696.tif 27 frames are being saved to U:\krios\20190717\PilA_6697.tif 27 frames are being saved to U:\krios\20190717\PilA_6698.tif Running script 17 at item 258 (62.68, 192.20) Remaining time to fill dewars is 4320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 36 65 20, reference by 23 155 150 20 Disparity in stage position after aligning to target position: 0.10 -0.21 (2nd move error -0.02 -0.09) Finished aligning to item in 1 rounds Trimming A by 20 50 55 20, reference by 20 36 65 20 -13.0 11.6 unbinned pixels; 59.4 29.3 nm along two shift axes; -42.2 -52.9 nm on specimen axes Total_Shift = 34.744337 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.58 microns changed by -3.08 to target drift = 0.07 nm/sec Measured defocus = -1.53 microns changed by 0.03 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6699.tif 27 frames are being saved to U:\krios\20190717\PilA_6700.tif 27 frames are being saved to U:\krios\20190717\PilA_6701.tif 27 frames are being saved to U:\krios\20190717\PilA_6702.tif 27 frames are being saved to U:\krios\20190717\PilA_6703.tif 27 frames are being saved to U:\krios\20190717\PilA_6704.tif Running script 17 at item 259 (61.14, 195.17) Remaining time to fill dewars is 4140 Dewars are NOT busy filling Trimming A by 210 22 170 20, reference by 211 20 20 210 Trimming A by 210 22 170 20, reference by 205 24 170 20 Trimming A by 210 22 170 20, reference by 24 151 20 190 Trimming A by 210 22 170 20, reference by 23 155 150 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.11 Trimming A by 25 20 20 20, reference by 205 24 170 20 Disparity in stage position after aligning to target position: 0.22 -0.25 (2nd move error 0.08 -0.16) Finished aligning to item in 2 rounds Trimming A by 20 20 20 20, reference by 25 20 20 20 -17.0 -2.4 unbinned pixels; 13.5 64.8 nm along two shift axes; 12.8 -65.7 nm on specimen axes Total_Shift = 34.36517 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.44 microns changed by -3.04 to target drift = 0.09 nm/sec Measured defocus = -1.68 microns changed by 0.08 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6705.tif 27 frames are being saved to U:\krios\20190717\PilA_6706.tif 27 frames are being saved to U:\krios\20190717\PilA_6707.tif 27 frames are being saved to U:\krios\20190717\PilA_6708.tif 27 frames are being saved to U:\krios\20190717\PilA_6709.tif 27 frames are being saved to U:\krios\20190717\PilA_6710.tif Running script 17 at item 260 (59.67, 197.76) Remaining time to fill dewars is 3960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 50 20 30 30, reference by 205 24 170 20 Disparity in stage position after aligning to target position: 0.22 -0.26 (2nd move error 0.07 -0.17) Finished aligning to item in 1 rounds Trimming A by 50 20 35 25, reference by 50 20 30 30 -21.4 -0.1 unbinned pixels; 27.7 77.3 nm along two shift axes; 4.8 -83.4 nm on specimen axes Total_Shift = 42.859624 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.25 microns changed by -2.95 to target drift = 0.09 nm/sec Measured defocus = -1.72 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6711.tif 27 frames are being saved to U:\krios\20190717\PilA_6712.tif 27 frames are being saved to U:\krios\20190717\PilA_6713.tif 27 frames are being saved to U:\krios\20190717\PilA_6714.tif 27 frames are being saved to U:\krios\20190717\PilA_6715.tif 27 frames are being saved to U:\krios\20190717\PilA_6716.tif Running script 17 at item 261 (58.28, 200.46) Remaining time to fill dewars is 3780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 125 22 20 85, reference by 205 24 170 20 Disparity in stage position after aligning to target position: 0.19 -0.28 (2nd move error 0.04 -0.19) Finished aligning to item in 1 rounds Trimming A by 120 20 20 85, reference by 125 22 20 85 -24.6 -3.3 unbinned pixels; 20.0 93.7 nm along two shift axes; 18.1 -95.1 nm on specimen axes Total_Shift = 49.705055 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.07 microns changed by -2.87 to target drift = 0.12 nm/sec Measured defocus = -1.88 microns changed by 0.08 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6717.tif 27 frames are being saved to U:\krios\20190717\PilA_6718.tif 27 frames are being saved to U:\krios\20190717\PilA_6719.tif 27 frames are being saved to U:\krios\20190717\PilA_6720.tif 27 frames are being saved to U:\krios\20190717\PilA_6721.tif 27 frames are being saved to U:\krios\20190717\PilA_6722.tif Running script 17 at item 262 (55.60, 198.66) Remaining time to fill dewars is 3660 Dewars are NOT busy filling Trimming A by 220 20 20 210, reference by 211 20 20 210 Trimming A by 220 20 20 210, reference by 205 24 170 20 Trimming A by 220 20 20 210, reference by 24 151 20 190 Trimming A by 220 20 20 210, reference by 23 155 150 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.04 Trimming A by 115 24 20 115, reference by 211 20 20 210 Disparity in stage position after aligning to target position: 0.33 0.10 (2nd move error 0.19 0.12) Finished aligning to item in 2 rounds Trimming A by 120 22 20 105, reference by 115 24 20 115 0.4 3.2 unbinned pixels; 11.3 6.2 nm along two shift axes; -12.6 0.8 nm on specimen axes Total_Shift = 6.45711 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.90 microns changed by -2.80 to target drift = 0.15 nm/sec Measured defocus = -1.88 microns changed by -0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6723.tif 27 frames are being saved to U:\krios\20190717\PilA_6724.tif 27 frames are being saved to U:\krios\20190717\PilA_6725.tif 27 frames are being saved to U:\krios\20190717\PilA_6726.tif 27 frames are being saved to U:\krios\20190717\PilA_6727.tif 27 frames are being saved to U:\krios\20190717\PilA_6728.tif Running script 17 at item 263 (56.89, 195.99) Remaining time to fill dewars is 3420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 20 20 55, reference by 211 20 20 210 Disparity in stage position after aligning to target position: 0.28 0.06 (2nd move error 0.15 0.08) Finished aligning to item in 1 rounds Trimming A by 50 21 20 55, reference by 55 20 20 55 1.2 -4.5 unbinned pixels; 18.3 2.6 nm along two shift axes; 17.4 5.5 nm on specimen axes Total_Shift = 9.393389 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.86 microns changed by -2.86 to target drift = 0.14 nm/sec Measured defocus = -2.02 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6729.tif 27 frames are being saved to U:\krios\20190717\PilA_6730.tif 27 frames are being saved to U:\krios\20190717\PilA_6731.tif 27 frames are being saved to U:\krios\20190717\PilA_6732.tif 27 frames are being saved to U:\krios\20190717\PilA_6733.tif 27 frames are being saved to U:\krios\20190717\PilA_6734.tif Running script 17 at item 264 (58.55, 193.22) Remaining time to fill dewars is 3240 Dewars are NOT busy filling Trimming A by 20 151 20 195, reference by 211 20 20 210 Trimming A by 20 151 20 195, reference by 205 24 170 20 Trimming A by 20 151 20 195, reference by 24 151 20 190 Trimming A by 20 151 20 195, reference by 23 155 150 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.06 Trimming A by 20 20 20 20, reference by 24 151 20 190 Disparity in stage position after aligning to target position: 0.17 0.15 (2nd move error 0.02 0.20) Finished aligning to item in 2 rounds 2.1 0.2 unbinned pixels; 1.9 7.9 nm along two shift axes; -1.3 8.1 nm on specimen axes Total_Shift = 4.230116 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.80 microns changed by -2.90 to target drift = 0.14 nm/sec Measured defocus = -2.05 microns changed by -0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6735.tif 27 frames are being saved to U:\krios\20190717\PilA_6736.tif 27 frames are being saved to U:\krios\20190717\PilA_6737.tif 27 frames are being saved to U:\krios\20190717\PilA_6738.tif 27 frames are being saved to U:\krios\20190717\PilA_6739.tif 27 frames are being saved to U:\krios\20190717\PilA_6740.tif Running script 17 at item 265 (60.03, 190.79) Remaining time to fill dewars is 3000 Dewars are NOT busy filling Trimming A by 23 155 145 20, reference by 211 20 20 210 Trimming A by 23 155 145 20, reference by 205 24 170 20 Trimming A by 23 155 145 20, reference by 24 151 20 190 Trimming A by 23 155 145 20, reference by 23 155 150 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.07 Trimming A by 20 30 25 20, reference by 23 155 150 20 Disparity in stage position after aligning to target position: -0.01 -0.27 (2nd move error -0.14 -0.23) Finished aligning to item in 2 rounds Trimming A by 20 30 25 20, reference by 20 30 25 20 -20.4 25.4 unbinned pixels; 120.1 35.4 nm along two shift axes; -94.5 -84.9 nm on specimen axes Total_Shift = 65.206533 Trimming A by 20 35 25 20, reference by 20 30 25 20 2.8 16.2 unbinned pixels; 55.9 34.3 nm along two shift axes; -63.5 7.3 nm on specimen axes Total_Shift = 32.832169 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.49 microns changed by -1.71 to target drift = 0.04 nm/sec Measured defocus = -2.23 microns changed by 0.03 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6741.tif 27 frames are being saved to U:\krios\20190717\PilA_6742.tif 27 frames are being saved to U:\krios\20190717\PilA_6743.tif 27 frames are being saved to U:\krios\20190717\PilA_6744.tif 27 frames are being saved to U:\krios\20190717\PilA_6745.tif 27 frames are being saved to U:\krios\20190717\PilA_6746.tif Running script 17 at item 266 (61.41, 188.09) Remaining time to fill dewars is 2880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 100 75 20, reference by 23 155 150 20 Disparity in stage position after aligning to target position: 0.09 -0.24 (2nd move error -0.04 -0.20) Finished aligning to item in 1 rounds Trimming A by 21 105 70 20, reference by 21 100 75 20 -24.1 9.4 unbinned pixels; 66.0 72.6 nm along two shift axes; -31.5 -95.7 nm on specimen axes Total_Shift = 51.717288 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.85 microns changed by -3.15 to target drift = 0.01 nm/sec Measured defocus = -2.39 microns changed by 0.09 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6747.tif 27 frames are being saved to U:\krios\20190717\PilA_6748.tif 27 frames are being saved to U:\krios\20190717\PilA_6749.tif 27 frames are being saved to U:\krios\20190717\PilA_6750.tif 27 frames are being saved to U:\krios\20190717\PilA_6751.tif 27 frames are being saved to U:\krios\20190717\PilA_6752.tif Running script 17 at item 267 (58.66, 186.28) Remaining time to fill dewars is 2640 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 130 20 100, reference by 24 151 20 190 Disparity in stage position after aligning to target position: 0.26 0.11 (2nd move error 0.10 0.15) Finished aligning to item in 1 rounds Trimming A by 22 130 20 95, reference by 22 130 20 100 -0.2 -0.9 unbinned pixels; 3.0 2.1 nm along two shift axes; 3.5 -0.6 nm on specimen axes Total_Shift = 1.842182 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.68 microns changed by -1.72 to target drift = 0.12 nm/sec Measured defocus = -2.40 microns changed by -0.00 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6753.tif 27 frames are being saved to U:\krios\20190717\PilA_6754.tif 27 frames are being saved to U:\krios\20190717\PilA_6755.tif 27 frames are being saved to U:\krios\20190717\PilA_6756.tif 27 frames are being saved to U:\krios\20190717\PilA_6757.tif 27 frames are being saved to U:\krios\20190717\PilA_6758.tif Running script 17 at item 268 (57.32, 189.00) Remaining time to fill dewars is 2520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 85 20 85, reference by 24 151 20 190 Disparity in stage position after aligning to target position: 0.20 0.06 (2nd move error 0.04 0.10) Finished aligning to item in 1 rounds Trimming A by 24 85 20 80, reference by 20 85 20 85 -0.6 -2.6 unbinned pixels; 8.8 6.2 nm along two shift axes; 10.3 -1.9 nm on specimen axes Total_Shift = 5.39528 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.52 microns changed by -3.02 to target drift = 0.14 nm/sec Measured defocus = -2.53 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6759.tif 27 frames are being saved to U:\krios\20190717\PilA_6760.tif 27 frames are being saved to U:\krios\20190717\PilA_6761.tif 27 frames are being saved to U:\krios\20190717\PilA_6762.tif 27 frames are being saved to U:\krios\20190717\PilA_6763.tif 27 frames are being saved to U:\krios\20190717\PilA_6764.tif Running script 17 at item 269 (55.89, 191.77) Remaining time to fill dewars is 2280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 55 20 70, reference by 24 151 20 190 Disparity in stage position after aligning to target position: 0.21 0.01 (2nd move error 0.06 0.05) Finished aligning to item in 1 rounds Trimming A by 23 45 20 75, reference by 21 55 20 70 -0.7 0.3 unbinned pixels; 1.9 2.1 nm along two shift axes; -0.9 -2.8 nm on specimen axes Total_Shift = 1.494268 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.97 microns changed by -1.63 to target drift = 0.13 nm/sec Measured defocus = -2.58 microns changed by -0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6765.tif 27 frames are being saved to U:\krios\20190717\PilA_6766.tif 27 frames are being saved to U:\krios\20190717\PilA_6767.tif 27 frames are being saved to U:\krios\20190717\PilA_6768.tif 27 frames are being saved to U:\krios\20190717\PilA_6769.tif 27 frames are being saved to U:\krios\20190717\PilA_6770.tif Running script 17 at item 270 (54.53, 194.56) Remaining time to fill dewars is 2160 Dewars are NOT busy filling Trimming A by 23 165 20 185, reference by 211 20 20 210 Trimming A by 23 165 20 185, reference by 205 24 170 20 Trimming A by 23 165 20 185, reference by 24 151 20 190 Trimming A by 23 165 20 185, reference by 23 155 150 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.06 Trimming A by 65 21 20 90, reference by 24 151 20 190 Disparity in stage position after aligning to target position: 0.17 0.02 (2nd move error -0.01 0.06) Finished aligning to item in 2 rounds Trimming A by 65 21 20 90, reference by 65 21 20 90 -6.9 28.2 unbinned pixels; 112.7 17.7 nm along two shift axes; -108.3 -32.7 nm on specimen axes Total_Shift = 58.08072 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.10 microns changed by -3.00 to target drift = 0.11 nm/sec Measured defocus = -0.87 microns changed by -0.03 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6771.tif 27 frames are being saved to U:\krios\20190717\PilA_6772.tif 27 frames are being saved to U:\krios\20190717\PilA_6773.tif 27 frames are being saved to U:\krios\20190717\PilA_6774.tif 27 frames are being saved to U:\krios\20190717\PilA_6775.tif 27 frames are being saved to U:\krios\20190717\PilA_6776.tif Running script 17 at item 271 (52.98, 197.28) Remaining time to fill dewars is 2160 Dewars are NOT busy filling Trimming A by 212 23 20 220, reference by 211 20 20 210 Trimming A by 212 23 20 220, reference by 205 24 170 20 Trimming A by 212 23 20 220, reference by 24 151 20 190 Trimming A by 212 23 20 220, reference by 23 155 150 20 Disparity in stage position after aligning to center of map frame: 0.20 -0.05 Trimming A by 108 24 20 170, reference by 211 20 20 210 Disparity in stage position after aligning to target position: 0.32 -0.04 (2nd move error 0.10 -0.01) Finished aligning to item in 2 rounds Trimming A by 112 20 20 165, reference by 108 24 20 170 -2.9 -3.0 unbinned pixels; 7.3 15.0 nm along two shift axes; 12.4 -10.6 nm on specimen axes Total_Shift = 8.383551 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.99 microns changed by -2.99 to target drift = 0.08 nm/sec Measured defocus = -0.96 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6777.tif 27 frames are being saved to U:\krios\20190717\PilA_6778.tif 27 frames are being saved to U:\krios\20190717\PilA_6779.tif 27 frames are being saved to U:\krios\20190717\PilA_6780.tif 27 frames are being saved to U:\krios\20190717\PilA_6781.tif 27 frames are being saved to U:\krios\20190717\PilA_6782.tif Running script 17 at item 272 (51.87, 193.17) Remaining time to fill dewars is 1860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 51 51 20 150, reference by 24 151 20 190 Disparity in stage position after aligning to target position: 0.13 -0.11 (2nd move error -0.05 -0.07) Finished aligning to item in 1 rounds Trimming A by 51 51 20 150, reference by 51 51 20 150 -8.1 10.4 unbinned pixels; 48.7 13.6 nm along two shift axes; -38.6 -33.8 nm on specimen axes Total_Shift = 26.336696 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.91 microns changed by -3.01 to target drift = 0.09 nm/sec Measured defocus = -1.05 microns changed by -0.05 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6783.tif 27 frames are being saved to U:\krios\20190717\PilA_6784.tif 27 frames are being saved to U:\krios\20190717\PilA_6785.tif 27 frames are being saved to U:\krios\20190717\PilA_6786.tif 27 frames are being saved to U:\krios\20190717\PilA_6787.tif 27 frames are being saved to U:\krios\20190717\PilA_6788.tif Running script 17 at item 273 (53.25, 190.32) Remaining time to fill dewars is 1560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 96 20 155, reference by 24 151 20 190 Disparity in stage position after aligning to target position: 0.22 -0.02 (2nd move error 0.04 0.02) Finished aligning to item in 1 rounds Trimming A by 21 105 20 145, reference by 21 96 20 155 1.8 -3.2 unbinned pixels; 14.1 1.7 nm along two shift axes; 12.0 7.6 nm on specimen axes Total_Shift = 7.314975 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.86 microns changed by -3.06 to target drift = 0.15 nm/sec Measured defocus = -1.21 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6789.tif 27 frames are being saved to U:\krios\20190717\PilA_6790.tif 27 frames are being saved to U:\krios\20190717\PilA_6791.tif 27 frames are being saved to U:\krios\20190717\PilA_6792.tif 27 frames are being saved to U:\krios\20190717\PilA_6793.tif 27 frames are being saved to U:\krios\20190717\PilA_6794.tif Running script 17 at item 274 (54.65, 187.62) Remaining time to fill dewars is 1440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 132 20 165, reference by 24 151 20 190 Disparity in stage position after aligning to target position: 0.31 0.03 (2nd move error 0.13 0.07) Finished aligning to item in 1 rounds Trimming A by 20 132 20 160, reference by 21 132 20 165 0.7 -0.2 unbinned pixels; 1.6 2.2 nm along two shift axes; 0.6 2.7 nm on specimen axes Total_Shift = 1.422082 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.78 microns changed by -3.08 to target drift = 0.13 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6795.tif 27 frames are being saved to U:\krios\20190717\PilA_6796.tif 27 frames are being saved to U:\krios\20190717\PilA_6797.tif 27 frames are being saved to U:\krios\20190717\PilA_6798.tif 27 frames are being saved to U:\krios\20190717\PilA_6799.tif 27 frames are being saved to U:\krios\20190717\PilA_6800.tif Running script 17 at item 275 (-78.51, 410.99) Remaining time to fill dewars is 1260 Dewars are NOT busy filling Trimming A by 23 170 160 20, reference by 235 24 20 220 Trimming A by 23 170 160 20, reference by 220 20 185 20 Trimming A by 23 170 160 20, reference by 24 180 20 205 Trimming A by 23 170 160 20, reference by 21 175 160 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.19 Trimming A by 46 65 110 20, reference by 21 175 160 20 Disparity in stage position after aligning to target position: -0.02 -0.19 (2nd move error -0.08 -0.02) Finished aligning to item in 2 rounds Trimming A by 46 65 110 20, reference by 46 65 110 20 -3.1 17.4 unbinned pixels; 68.1 14.9 nm along two shift axes; -67.1 -15.9 nm on specimen axes Total_Shift = 35.376299 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.26 microns changed by -2.66 to target drift = 0.11 nm/sec Measured defocus = -1.40 microns changed by -0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6801.tif 27 frames are being saved to U:\krios\20190717\PilA_6802.tif 27 frames are being saved to U:\krios\20190717\PilA_6803.tif 27 frames are being saved to U:\krios\20190717\PilA_6804.tif 27 frames are being saved to U:\krios\20190717\PilA_6805.tif 27 frames are being saved to U:\krios\20190717\PilA_6806.tif Running script 17 at item 276 (-80.12, 413.75) Remaining time to fill dewars is 1080 Dewars are NOT busy filling Trimming A by 231 24 180 20, reference by 235 24 20 220 Trimming A by 231 24 180 20, reference by 220 20 185 20 Trimming A by 231 24 180 20, reference by 24 180 20 205 Trimming A by 231 24 180 20, reference by 21 175 160 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.18 Trimming A by 95 24 100 20, reference by 220 20 185 20 Disparity in stage position after aligning to target position: 0.19 -0.19 (2nd move error 0.09 -0.03) Finished aligning to item in 2 rounds Trimming A by 95 24 105 20, reference by 95 24 100 20 -3.8 -0.8 unbinned pixels; 2.0 15.1 nm along two shift axes; 4.0 -14.7 nm on specimen axes Total_Shift = 7.847521 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.06 microns changed by -2.56 to target drift = 0.09 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6807.tif 27 frames are being saved to U:\krios\20190717\PilA_6808.tif 27 frames are being saved to U:\krios\20190717\PilA_6809.tif 27 frames are being saved to U:\krios\20190717\PilA_6810.tif 27 frames are being saved to U:\krios\20190717\PilA_6811.tif 27 frames are being saved to U:\krios\20190717\PilA_6812.tif Running script 17 at item 277 (-81.51, 416.45) Remaining time to fill dewars is 900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 24 110 20, reference by 220 20 185 20 Disparity in stage position after aligning to target position: 0.20 -0.22 (2nd move error 0.09 -0.05) Finished aligning to item in 1 rounds Trimming A by 135 24 110 20, reference by 135 24 110 20 -7.6 -4.8 unbinned pixels; 7.7 34.4 nm along two shift axes; 20.2 -28.4 nm on specimen axes Total_Shift = 17.883839 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.94 microns changed by -2.54 to target drift = 0.13 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6813.tif 27 frames are being saved to U:\krios\20190717\PilA_6814.tif 27 frames are being saved to U:\krios\20190717\PilA_6815.tif 27 frames are being saved to U:\krios\20190717\PilA_6816.tif 27 frames are being saved to U:\krios\20190717\PilA_6817.tif 27 frames are being saved to U:\krios\20190717\PilA_6818.tif Running script 17 at item 278 (-84.14, 415.08) Remaining time to fill dewars is 720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 20 50 45, reference by 220 20 185 20 Disparity in stage position after aligning to target position: 0.18 -0.26 (2nd move error 0.08 -0.09) Finished aligning to item in 1 rounds Trimming A by 70 20 50 40, reference by 70 20 50 45 -12.8 -3.4 unbinned pixels; 4.1 51.2 nm along two shift axes; 16.0 -49.0 nm on specimen axes Total_Shift = 26.479931 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.91 microns changed by -2.61 to target drift = 0.11 nm/sec Measured defocus = -1.70 microns changed by 0.00 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6819.tif 27 frames are being saved to U:\krios\20190717\PilA_6820.tif 27 frames are being saved to U:\krios\20190717\PilA_6821.tif 27 frames are being saved to U:\krios\20190717\PilA_6822.tif 27 frames are being saved to U:\krios\20190717\PilA_6823.tif 27 frames are being saved to U:\krios\20190717\PilA_6824.tif Running script 17 at item 279 (-82.76, 412.35) Remaining time to fill dewars is 420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 21 30 20, reference by 220 20 185 20 Disparity in stage position after aligning to target position: 0.32 -0.25 (2nd move error 0.22 -0.09) Finished aligning to item in 1 rounds Trimming A by 35 21 35 20, reference by 35 21 30 20 -8.2 -4.0 unbinned pixels; 4.1 35.4 nm along two shift axes; 17.3 -30.9 nm on specimen axes Total_Shift = 18.178176 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.83 microns changed by -2.63 to target drift = 0.15 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6825.tif 27 frames are being saved to U:\krios\20190717\PilA_6826.tif 27 frames are being saved to U:\krios\20190717\PilA_6827.tif 27 frames are being saved to U:\krios\20190717\PilA_6828.tif 27 frames are being saved to U:\krios\20190717\PilA_6829.tif 27 frames are being saved to U:\krios\20190717\PilA_6830.tif Running script 17 at item 280 (-81.15, 409.54) Remaining time to fill dewars is 360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 42 75 20, reference by 21 175 160 20 Disparity in stage position after aligning to target position: 0.13 -0.20 (2nd move error 0.07 -0.04) Finished aligning to item in 1 rounds Trimming A by 24 60 60 20, reference by 22 42 75 20 -5.0 -1.8 unbinned pixels; 0.2 20.7 nm along two shift axes; 8.1 -19.0 nm on specimen axes Total_Shift = 10.620727 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.74 microns changed by -2.64 to target drift = 0.08 nm/sec Measured defocus = -1.97 microns changed by 0.07 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6831.tif 27 frames are being saved to U:\krios\20190717\PilA_6832.tif 27 frames are being saved to U:\krios\20190717\PilA_6833.tif 27 frames are being saved to U:\krios\20190717\PilA_6834.tif 27 frames are being saved to U:\krios\20190717\PilA_6835.tif 27 frames are being saved to U:\krios\20190717\PilA_6836.tif Running script 17 at item 281 (-79.72, 406.77) Remaining time to fill dewars is 120 Dewars are NOT busy filling Trimming A by 23 170 155 20, reference by 235 24 20 220 Trimming A by 23 170 155 20, reference by 220 20 185 20 Trimming A by 23 170 155 20, reference by 24 180 20 205 Trimming A by 23 170 155 20, reference by 21 175 160 20 Disparity in stage position after aligning to center of map frame: 0.16 -0.15 Trimming A by 22 125 120 20, reference by 21 175 160 20 Disparity in stage position after aligning to target position: 0.16 -0.21 (2nd move error -0.01 -0.08) Finished aligning to item in 2 rounds Trimming A by 20 130 120 20, reference by 22 125 120 20 -11.4 8.8 unbinned pixels; 47.2 27.7 nm along two shift axes; -31.9 -46.1 nm on specimen axes Total_Shift = 28.759957 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.67 microns changed by -2.67 to target drift = 0.05 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6837.tif 27 frames are being saved to U:\krios\20190717\PilA_6838.tif 27 frames are being saved to U:\krios\20190717\PilA_6839.tif 27 frames are being saved to U:\krios\20190717\PilA_6840.tif 27 frames are being saved to U:\krios\20190717\PilA_6841.tif 27 frames are being saved to U:\krios\20190717\PilA_6842.tif Running script 17 at item 282 (-82.34, 405.39) Remaining time to fill dewars is -2160 Dewars ARE busy filling Dewars ARE busy filling Dewars ARE busy filling Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 115 75 20, reference by 21 175 160 20 Disparity in stage position after aligning to target position: 0.26 -0.28 (2nd move error 0.10 -0.15) Finished aligning to item in 1 rounds Trimming A by 20 120 75 20, reference by 20 115 75 20 -16.6 0.6 unbinned pixels; 23.8 59.1 nm along two shift axes; 1.4 -64.9 nm on specimen axes Total_Shift = 33.310106 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.62 microns changed by -2.72 to target drift = 0.15 nm/sec Measured defocus = -2.09 microns changed by -0.01 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6843.tif 27 frames are being saved to U:\krios\20190717\PilA_6844.tif 27 frames are being saved to U:\krios\20190717\PilA_6845.tif 27 frames are being saved to U:\krios\20190717\PilA_6846.tif 27 frames are being saved to U:\krios\20190717\PilA_6847.tif 27 frames are being saved to U:\krios\20190717\PilA_6848.tif Running script 17 at item 283 (-83.74, 408.18) Remaining time to fill dewars is 31260 Dewars are NOT busy filling Trimming A by 23 170 155 20, reference by 235 24 20 220 Trimming A by 23 170 155 20, reference by 220 20 185 20 Trimming A by 23 170 155 20, reference by 24 180 20 205 Trimming A by 23 170 155 20, reference by 21 175 160 20 Disparity in stage position after aligning to center of map frame: 0.16 -0.14 Trimming A by 20 40 30 25, reference by 21 175 160 20 Disparity in stage position after aligning to target position: 0.12 -0.31 (2nd move error -0.04 -0.19) Finished aligning to item in 2 rounds Trimming A by 20 40 30 30, reference by 20 40 30 25 -24.7 10.0 unbinned pixels; 69.0 73.7 nm along two shift axes; -33.7 -98.1 nm on specimen axes Total_Shift = 53.233591 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.54 microns changed by -2.74 to target drift = 0.03 nm/sec Measured defocus = -2.27 microns changed by 0.07 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6849.tif 27 frames are being saved to U:\krios\20190717\PilA_6850.tif 27 frames are being saved to U:\krios\20190717\PilA_6851.tif 27 frames are being saved to U:\krios\20190717\PilA_6852.tif 27 frames are being saved to U:\krios\20190717\PilA_6853.tif 27 frames are being saved to U:\krios\20190717\PilA_6854.tif Running script 17 at item 284 (-85.38, 410.83) Remaining time to fill dewars is 31140 Dewars are NOT busy filling Trimming A by 22 175 20 215, reference by 235 24 20 220 Trimming A by 22 175 20 215, reference by 220 20 185 20 Trimming A by 22 175 20 215, reference by 24 180 20 205 Trimming A by 22 175 20 215, reference by 21 175 160 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.08 Trimming A by 20 20 20 20, reference by 24 180 20 205 Disparity in stage position after aligning to target position: 0.19 0.03 (2nd move error 0.07 0.10) Finished aligning to item in 2 rounds -2.4 1.2 unbinned pixels; 7.5 6.9 nm along two shift axes; -4.1 -9.6 nm on specimen axes Total_Shift = 5.370728 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.31 microns changed by -2.61 to target drift = 0.13 nm/sec Measured defocus = -2.28 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6855.tif 27 frames are being saved to U:\krios\20190717\PilA_6856.tif 27 frames are being saved to U:\krios\20190717\PilA_6857.tif 27 frames are being saved to U:\krios\20190717\PilA_6858.tif 27 frames are being saved to U:\krios\20190717\PilA_6859.tif 27 frames are being saved to U:\krios\20190717\PilA_6860.tif Running script 17 at item 285 (-86.69, 413.32) Remaining time to fill dewars is 31020 Dewars are NOT busy filling Trimming A by 240 22 20 225, reference by 235 24 20 220 Trimming A by 240 22 20 225, reference by 220 20 185 20 Trimming A by 240 22 20 225, reference by 24 180 20 205 Trimming A by 240 22 20 225, reference by 21 175 160 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.08 Trimming A by 65 21 20 70, reference by 235 24 20 220 Disparity in stage position after aligning to target position: 0.30 0.11 (2nd move error 0.15 0.17) Finished aligning to item in 2 rounds Trimming A by 70 20 20 70, reference by 65 21 20 70 1.1 -2.3 unbinned pixels; 10.0 0.5 nm along two shift axes; 8.8 4.8 nm on specimen axes Total_Shift = 5.135246 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -1.12 microns changed by -1.28 to target drift = 0.17 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6861.tif 27 frames are being saved to U:\krios\20190717\PilA_6862.tif 27 frames are being saved to U:\krios\20190717\PilA_6863.tif 27 frames are being saved to U:\krios\20190717\PilA_6864.tif 27 frames are being saved to U:\krios\20190717\PilA_6865.tif 27 frames are being saved to U:\krios\20190717\PilA_6866.tif Running script 17 at item 286 (-89.36, 411.92) Remaining time to fill dewars is 30840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 75 21 20 115, reference by 235 24 20 220 Disparity in stage position after aligning to target position: 0.37 -0.10 (2nd move error 0.22 -0.04) Finished aligning to item in 1 rounds Trimming A by 75 21 20 110, reference by 75 21 20 115 -2.6 -2.6 unbinned pixels; 6.3 13.2 nm along two shift axes; 10.7 -9.4 nm on specimen axes Total_Shift = 7.326078 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.25 microns changed by -2.75 to target drift = 0.12 nm/sec Measured defocus = -2.58 microns changed by 0.08 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6867.tif 27 frames are being saved to U:\krios\20190717\PilA_6868.tif 27 frames are being saved to U:\krios\20190717\PilA_6869.tif 27 frames are being saved to U:\krios\20190717\PilA_6870.tif 27 frames are being saved to U:\krios\20190717\PilA_6871.tif 27 frames are being saved to U:\krios\20190717\PilA_6872.tif Running script 17 at item 287 (-87.82, 409.16) Remaining time to fill dewars is 30720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 65 20 90, reference by 24 180 20 205 Disparity in stage position after aligning to target position: 0.19 -0.05 (2nd move error 0.07 0.02) Finished aligning to item in 1 rounds Trimming A by 22 52 20 100, reference by 21 65 20 90 1.6 -0.5 unbinned pixels; 3.9 5.0 nm along two shift axes; 1.6 6.3 nm on specimen axes Total_Shift = 3.353443 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -1.26 microns changed by -1.34 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6873.tif 27 frames are being saved to U:\krios\20190717\PilA_6874.tif 27 frames are being saved to U:\krios\20190717\PilA_6875.tif 27 frames are being saved to U:\krios\20190717\PilA_6876.tif 27 frames are being saved to U:\krios\20190717\PilA_6877.tif 27 frames are being saved to U:\krios\20190717\PilA_6878.tif Running script 17 at item 288 (-86.44, 406.40) Remaining time to fill dewars is 30660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 91 20 115, reference by 24 180 20 205 Disparity in stage position after aligning to target position: 0.35 -0.02 (2nd move error 0.23 0.04) Finished aligning to item in 1 rounds Trimming A by 20 100 20 100, reference by 20 91 20 115 1.2 -1.8 unbinned pixels; 8.0 1.5 nm along two shift axes; 6.6 4.9 nm on specimen axes Total_Shift = 4.225346 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.85 microns changed by -2.75 to target drift = 0.15 nm/sec Measured defocus = -0.83 microns changed by -0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6879.tif 27 frames are being saved to U:\krios\20190717\PilA_6880.tif 27 frames are being saved to U:\krios\20190717\PilA_6881.tif 27 frames are being saved to U:\krios\20190717\PilA_6882.tif 27 frames are being saved to U:\krios\20190717\PilA_6883.tif 27 frames are being saved to U:\krios\20190717\PilA_6884.tif Running script 17 at item 289 (-85.21, 403.83) Remaining time to fill dewars is 30660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 150 20 115, reference by 24 180 20 205 Disparity in stage position after aligning to target position: 0.34 0.01 (2nd move error 0.21 0.07) Finished aligning to item in 1 rounds Trimming A by 22 145 20 110, reference by 20 150 20 115 1.7 10.6 unbinned pixels; 36.7 21.9 nm along two shift axes; -41.5 4.3 nm on specimen axes Total_Shift = 21.417319 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.80 microns changed by -2.80 to target drift = 0.16 nm/sec Measured defocus = -1.00 microns changed by 0.00 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6885.tif 27 frames are being saved to U:\krios\20190717\PilA_6886.tif 27 frames are being saved to U:\krios\20190717\PilA_6887.tif 27 frames are being saved to U:\krios\20190717\PilA_6888.tif 27 frames are being saved to U:\krios\20190717\PilA_6889.tif 27 frames are being saved to U:\krios\20190717\PilA_6890.tif Running script 17 at item 290 (-117.94, 364.42) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 20 135 120 20, reference by 240 24 20 240 Trimming A by 20 135 120 20, reference by 210 24 160 20 Trimming A by 20 135 120 20, reference by 21 161 20 215 Trimming A by 20 135 120 20, reference by 24 140 125 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.18 Trimming A by 75 41 120 20, reference by 24 140 125 20 Disparity in stage position after aligning to target position: 0.03 -0.10 (2nd move error -0.07 0.05) Finished aligning to item in 2 rounds Trimming A by 80 41 120 20, reference by 75 41 120 20 1.7 15.0 unbinned pixels; 53.1 28.7 nm along two shift axes; -58.8 3.4 nm on specimen axes Total_Shift = 30.249695 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.67 microns changed by -2.77 to target drift = 0.20 nm/sec Measured defocus = -1.13 microns changed by 0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6891.tif 27 frames are being saved to U:\krios\20190717\PilA_6892.tif 27 frames are being saved to U:\krios\20190717\PilA_6893.tif 27 frames are being saved to U:\krios\20190717\PilA_6894.tif 27 frames are being saved to U:\krios\20190717\PilA_6895.tif 27 frames are being saved to U:\krios\20190717\PilA_6896.tif Running script 17 at item 291 (-119.55, 367.11) Remaining time to fill dewars is 30300 Dewars are NOT busy filling Trimming A by 220 22 160 20, reference by 240 24 20 240 Trimming A by 220 22 160 20, reference by 210 24 160 20 Trimming A by 220 22 160 20, reference by 21 161 20 215 Trimming A by 220 22 160 20, reference by 24 140 125 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.19 Trimming A by 115 23 125 20, reference by 210 24 160 20 Disparity in stage position after aligning to target position: 0.26 -0.17 (2nd move error 0.13 -0.00) Finished aligning to item in 2 rounds Trimming A by 110 20 130 20, reference by 115 23 125 20 -0.9 -3.0 unbinned pixels; 9.8 7.9 nm along two shift axes; 11.9 -3.0 nm on specimen axes Total_Shift = 6.28845 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.43 microns changed by -2.63 to target drift = 0.11 nm/sec Measured defocus = -1.23 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6897.tif 27 frames are being saved to U:\krios\20190717\PilA_6898.tif 27 frames are being saved to U:\krios\20190717\PilA_6899.tif 27 frames are being saved to U:\krios\20190717\PilA_6900.tif 27 frames are being saved to U:\krios\20190717\PilA_6901.tif 27 frames are being saved to U:\krios\20190717\PilA_6902.tif Running script 17 at item 292 (-125.02, 371.18) Remaining time to fill dewars is 30120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 150 20 65 75, reference by 210 24 160 20 Disparity in stage position after aligning to target position: 0.21 -0.23 (2nd move error 0.07 -0.06) Finished aligning to item in 1 rounds Trimming A by 150 20 65 70, reference by 150 20 65 75 -8.6 -0.5 unbinned pixels; 9.5 31.8 nm along two shift axes; 3.7 -33.5 nm on specimen axes Total_Shift = 17.321846 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.32 microns changed by -2.62 to target drift = 0.10 nm/sec Measured defocus = -1.30 microns changed by -0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6903.tif 27 frames are being saved to U:\krios\20190717\PilA_6904.tif 27 frames are being saved to U:\krios\20190717\PilA_6905.tif 27 frames are being saved to U:\krios\20190717\PilA_6906.tif 27 frames are being saved to U:\krios\20190717\PilA_6907.tif 27 frames are being saved to U:\krios\20190717\PilA_6908.tif Running script 17 at item 293 (-123.57, 368.37) Remaining time to fill dewars is 30000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 81 21 75 20, reference by 210 24 160 20 Disparity in stage position after aligning to target position: 0.23 -0.21 (2nd move error 0.09 -0.04) Finished aligning to item in 1 rounds Trimming A by 95 22 65 20, reference by 81 21 75 20 -5.2 -1.0 unbinned pixels; 3.2 20.1 nm along two shift axes; 4.9 -20.0 nm on specimen axes Total_Shift = 10.557823 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.22 microns changed by -2.62 to target drift = 0.10 nm/sec Measured defocus = -1.44 microns changed by 0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6909.tif 27 frames are being saved to U:\krios\20190717\PilA_6910.tif 27 frames are being saved to U:\krios\20190717\PilA_6911.tif 27 frames are being saved to U:\krios\20190717\PilA_6912.tif 27 frames are being saved to U:\krios\20190717\PilA_6913.tif 27 frames are being saved to U:\krios\20190717\PilA_6914.tif Running script 17 at item 294 (-122.21, 365.64) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 20 45 20, reference by 210 24 160 20 Disparity in stage position after aligning to target position: 0.35 -0.18 (2nd move error 0.23 -0.00) Finished aligning to item in 1 rounds Trimming A by 55 20 50 20, reference by 55 20 45 20 0.9 -4.3 unbinned pixels; 16.8 3.3 nm along two shift axes; 16.4 4.3 nm on specimen axes Total_Shift = 8.719934 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.12 microns changed by -2.62 to target drift = 0.15 nm/sec Measured defocus = -1.48 microns changed by -0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6915.tif 27 frames are being saved to U:\krios\20190717\PilA_6916.tif 27 frames are being saved to U:\krios\20190717\PilA_6917.tif 27 frames are being saved to U:\krios\20190717\PilA_6918.tif 27 frames are being saved to U:\krios\20190717\PilA_6919.tif 27 frames are being saved to U:\krios\20190717\PilA_6920.tif Running script 17 at item 295 (-120.61, 362.95) Remaining time to fill dewars is 29700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 45 55 20, reference by 24 140 125 20 Disparity in stage position after aligning to target position: 0.13 -0.22 (2nd move error 0.04 -0.06) Finished aligning to item in 1 rounds Trimming A by 20 31 70 20, reference by 20 45 55 20 -9.1 -2.7 unbinned pixels; 1.8 36.7 nm along two shift axes; 12.5 -34.6 nm on specimen axes Total_Shift = 18.902685 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.05 microns changed by -2.65 to target drift = 0.13 nm/sec Measured defocus = -1.60 microns changed by -0.00 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6921.tif 27 frames are being saved to U:\krios\20190717\PilA_6922.tif 27 frames are being saved to U:\krios\20190717\PilA_6923.tif 27 frames are being saved to U:\krios\20190717\PilA_6924.tif 27 frames are being saved to U:\krios\20190717\PilA_6925.tif 27 frames are being saved to U:\krios\20190717\PilA_6926.tif Running script 17 at item 296 (-119.22, 360.17) Remaining time to fill dewars is 29700 Dewars are NOT busy filling Trimming A by 22 121 130 20, reference by 240 24 20 240 Trimming A by 22 121 130 20, reference by 210 24 160 20 Trimming A by 22 121 130 20, reference by 21 161 20 215 Trimming A by 22 121 130 20, reference by 24 140 125 20 Disparity in stage position after aligning to center of map frame: 0.20 -0.20 Trimming A by 23 101 125 20, reference by 24 140 125 20 Disparity in stage position after aligning to target position: 0.23 -0.14 (2nd move error 0.02 0.03) Finished aligning to item in 2 rounds Trimming A by 23 101 125 20, reference by 23 101 125 20 -2.1 -0.5 unbinned pixels; 1.1 8.3 nm along two shift axes; 2.2 -8.2 nm on specimen axes Total_Shift = 4.340564 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.95 microns changed by -2.65 to target drift = 0.16 nm/sec Measured defocus = -1.68 microns changed by -0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6927.tif 27 frames are being saved to U:\krios\20190717\PilA_6928.tif 27 frames are being saved to U:\krios\20190717\PilA_6929.tif 27 frames are being saved to U:\krios\20190717\PilA_6930.tif 27 frames are being saved to U:\krios\20190717\PilA_6931.tif 27 frames are being saved to U:\krios\20190717\PilA_6932.tif Running script 17 at item 297 (-121.88, 358.80) Remaining time to fill dewars is 29520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 95 75 20, reference by 24 140 125 20 Disparity in stage position after aligning to target position: 0.26 -0.29 (2nd move error 0.06 -0.11) Finished aligning to item in 1 rounds Trimming A by 22 95 80 20, reference by 22 95 75 20 -14.5 -1.7 unbinned pixels; 12.6 54.8 nm along two shift axes; 9.7 -56.0 nm on specimen axes Total_Shift = 29.196245 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.92 microns changed by -2.72 to target drift = 0.12 nm/sec Measured defocus = -1.84 microns changed by 0.04 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6933.tif 27 frames are being saved to U:\krios\20190717\PilA_6934.tif 27 frames are being saved to U:\krios\20190717\PilA_6935.tif 27 frames are being saved to U:\krios\20190717\PilA_6936.tif 27 frames are being saved to U:\krios\20190717\PilA_6937.tif 27 frames are being saved to U:\krios\20190717\PilA_6938.tif Running script 17 at item 298 (-123.28, 361.56) Remaining time to fill dewars is 29400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 25 20 20, reference by 24 140 125 20 Disparity in stage position after aligning to target position: 0.17 -0.29 (2nd move error -0.03 -0.12) Finished aligning to item in 1 rounds Trimming A by 20 30 20 20, reference by 20 25 20 20 -15.7 7.5 unbinned pixels; 47.9 45.2 nm along two shift axes; -25.7 -62.6 nm on specimen axes Total_Shift = 34.720053 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.75 microns changed by -2.65 to target drift = 0.02 nm/sec Measured defocus = -1.87 microns changed by -0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6939.tif 27 frames are being saved to U:\krios\20190717\PilA_6940.tif 27 frames are being saved to U:\krios\20190717\PilA_6941.tif 27 frames are being saved to U:\krios\20190717\PilA_6942.tif 27 frames are being saved to U:\krios\20190717\PilA_6943.tif 27 frames are being saved to U:\krios\20190717\PilA_6944.tif Running script 17 at item 299 (-126.29, 367.04) Remaining time to fill dewars is 29160 Dewars are NOT busy filling Trimming A by 215 24 160 20, reference by 240 24 20 240 Trimming A by 215 24 160 20, reference by 210 24 160 20 Trimming A by 215 24 160 20, reference by 21 161 20 215 Trimming A by 215 24 160 20, reference by 24 140 125 20 Disparity in stage position after aligning to center of map frame: 0.18 -0.13 Trimming A by 65 22 20 70, reference by 210 24 160 20 Disparity in stage position after aligning to target position: 0.33 -0.33 (2nd move error 0.14 -0.21) Finished aligning to item in 2 rounds Trimming A by 65 20 20 65, reference by 65 22 20 70 -15.2 -7.4 unbinned pixels; 7.4 65.7 nm along two shift axes; 31.9 -57.4 nm on specimen axes Total_Shift = 33.722005 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.73 microns changed by -2.73 to target drift = 0.19 nm/sec Measured defocus = -2.12 microns changed by 0.12 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6945.tif 27 frames are being saved to U:\krios\20190717\PilA_6946.tif 27 frames are being saved to U:\krios\20190717\PilA_6947.tif 27 frames are being saved to U:\krios\20190717\PilA_6948.tif 27 frames are being saved to U:\krios\20190717\PilA_6949.tif 27 frames are being saved to U:\krios\20190717\PilA_6950.tif Running script 17 at item 300 (-128.83, 365.30) Remaining time to fill dewars is 29100 Dewars are NOT busy filling Trimming A by 250 22 20 240, reference by 240 24 20 240 Trimming A by 250 22 20 240, reference by 210 24 160 20 Trimming A by 250 22 20 240, reference by 21 161 20 215 Trimming A by 250 22 20 240, reference by 24 140 125 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.12 Trimming A by 75 20 20 90, reference by 240 24 20 240 Disparity in stage position after aligning to target position: 0.30 -0.08 (2nd move error 0.17 0.04) Finished aligning to item in 2 rounds Trimming A by 57 22 20 105, reference by 75 20 20 90 -1.7 -20.6 unbinned pixels; 73.7 37.2 nm along two shift axes; 80.7 -2.2 nm on specimen axes Total_Shift = 41.421355 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.51 microns changed by -2.61 to target drift = 0.18 nm/sec Measured defocus = -2.07 microns changed by -0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6951.tif 27 frames are being saved to U:\krios\20190717\PilA_6952.tif 27 frames are being saved to U:\krios\20190717\PilA_6953.tif 27 frames are being saved to U:\krios\20190717\PilA_6954.tif 27 frames are being saved to U:\krios\20190717\PilA_6955.tif 27 frames are being saved to U:\krios\20190717\PilA_6956.tif Running script 17 at item 301 (-127.38, 362.54) Remaining time to fill dewars is 28860 Dewars are NOT busy filling Trimming A by 22 165 20 215, reference by 240 24 20 240 Trimming A by 22 165 20 215, reference by 210 24 160 20 Trimming A by 22 165 20 215, reference by 21 161 20 215 Trimming A by 22 165 20 215, reference by 24 140 125 20 Disparity in stage position after aligning to center of map frame: 0.16 -0.15 Trimming A by 21 25 20 55, reference by 21 161 20 215 Disparity in stage position after aligning to target position: 0.23 0.06 (2nd move error 0.05 0.20) Finished aligning to item in 2 rounds Trimming A by 20 30 20 40, reference by 21 25 20 55 -6.0 1.0 unbinned pixels; 11.5 20.2 nm along two shift axes; -2.6 -23.7 nm on specimen axes Total_Shift = 12.223605 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.46 microns changed by -2.66 to target drift = 0.13 nm/sec Measured defocus = -2.23 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6957.tif 27 frames are being saved to U:\krios\20190717\PilA_6958.tif 27 frames are being saved to U:\krios\20190717\PilA_6959.tif 27 frames are being saved to U:\krios\20190717\PilA_6960.tif 27 frames are being saved to U:\krios\20190717\PilA_6961.tif 27 frames are being saved to U:\krios\20190717\PilA_6962.tif Running script 17 at item 302 (-124.47, 357.35) Remaining time to fill dewars is 28860 Dewars are NOT busy filling Trimming A by 20 135 120 20, reference by 240 24 20 240 Trimming A by 20 135 120 20, reference by 210 24 160 20 Trimming A by 20 135 120 20, reference by 21 161 20 215 Trimming A by 20 135 120 20, reference by 24 140 125 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.11 Trimming A by 20 95 30 70, reference by 24 140 125 20 Disparity in stage position after aligning to target position: 0.21 -0.30 (2nd move error 0.07 -0.21) Finished aligning to item in 2 rounds Trimming A by 21 95 40 75, reference by 20 95 30 70 -20.6 -0.3 unbinned pixels; 25.9 74.6 nm along two shift axes; 5.4 -80.1 nm on specimen axes Total_Shift = 41.19599 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.38 microns changed by -2.68 to target drift = 0.15 nm/sec Measured defocus = -2.30 microns changed by 0.00 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6963.tif 27 frames are being saved to U:\krios\20190717\PilA_6964.tif 27 frames are being saved to U:\krios\20190717\PilA_6965.tif 27 frames are being saved to U:\krios\20190717\PilA_6966.tif 27 frames are being saved to U:\krios\20190717\PilA_6967.tif 27 frames are being saved to U:\krios\20190717\PilA_6968.tif Running script 17 at item 303 (-127.21, 355.63) Remaining time to fill dewars is 28680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 152 20 155, reference by 21 161 20 215 Disparity in stage position after aligning to target position: 0.30 -0.03 (2nd move error 0.13 0.10) Finished aligning to item in 1 rounds Trimming A by 23 165 20 135, reference by 24 152 20 155 0.1 1.3 unbinned pixels; 4.5 2.2 nm along two shift axes; -4.9 0.1 nm on specimen axes Total_Shift = 2.525894 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -1.03 microns changed by -1.37 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6969.tif 27 frames are being saved to U:\krios\20190717\PilA_6970.tif 27 frames are being saved to U:\krios\20190717\PilA_6971.tif 27 frames are being saved to U:\krios\20190717\PilA_6972.tif 27 frames are being saved to U:\krios\20190717\PilA_6973.tif 27 frames are being saved to U:\krios\20190717\PilA_6974.tif Running script 17 at item 304 (-128.56, 358.40) Remaining time to fill dewars is 28440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 120 20 130, reference by 21 161 20 215 Disparity in stage position after aligning to target position: 0.24 -0.10 (2nd move error 0.07 0.04) Finished aligning to item in 1 rounds Trimming A by 20 106 20 140, reference by 24 120 20 130 0.2 -1.1 unbinned pixels; 4.5 0.8 nm along two shift axes; 4.3 1.2 nm on specimen axes Total_Shift = 2.307679 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.23 microns changed by -2.73 to target drift = 0.11 nm/sec Measured defocus = -2.55 microns changed by 0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6975.tif 27 frames are being saved to U:\krios\20190717\PilA_6976.tif 27 frames are being saved to U:\krios\20190717\PilA_6977.tif 27 frames are being saved to U:\krios\20190717\PilA_6978.tif 27 frames are being saved to U:\krios\20190717\PilA_6979.tif 27 frames are being saved to U:\krios\20190717\PilA_6980.tif Running script 17 at item 305 (-129.96, 361.13) Remaining time to fill dewars is 28380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 85 20 115, reference by 21 161 20 215 Disparity in stage position after aligning to target position: 0.27 -0.11 (2nd move error 0.10 0.04) Finished aligning to item in 1 rounds Trimming A by 24 71 20 125, reference by 20 85 20 115 -0.2 -1.4 unbinned pixels; 4.7 2.8 nm along two shift axes; 5.3 -0.6 nm on specimen axes Total_Shift = 2.75146 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -1.30 microns changed by -1.30 to target drift = 0.16 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6981.tif 27 frames are being saved to U:\krios\20190717\PilA_6982.tif 27 frames are being saved to U:\krios\20190717\PilA_6983.tif 27 frames are being saved to U:\krios\20190717\PilA_6984.tif 27 frames are being saved to U:\krios\20190717\PilA_6985.tif 27 frames are being saved to U:\krios\20190717\PilA_6986.tif Running script 17 at item 306 (-131.36, 363.95) Remaining time to fill dewars is 28260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 53 22 20 155, reference by 21 161 20 215 Disparity in stage position after aligning to target position: 0.13 -0.08 (2nd move error -0.04 0.07) Finished aligning to item in 1 rounds Trimming A by 62 22 20 150, reference by 53 22 20 155 -2.8 15.1 unbinned pixels; 59.3 12.9 nm along two shift axes; -58.3 -13.9 nm on specimen axes Total_Shift = 30.784217 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.83 microns changed by -2.73 to target drift = 0.10 nm/sec Measured defocus = -0.89 microns changed by -0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6987.tif 27 frames are being saved to U:\krios\20190717\PilA_6988.tif 27 frames are being saved to U:\krios\20190717\PilA_6989.tif 27 frames are being saved to U:\krios\20190717\PilA_6990.tif 27 frames are being saved to U:\krios\20190717\PilA_6991.tif 27 frames are being saved to U:\krios\20190717\PilA_6992.tif Running script 17 at item 307 (-654.65, 601.36) Remaining time to fill dewars is 28140 Dewars are NOT busy filling Trimming A by 24 110 95 20, reference by 200 21 20 270 Trimming A by 24 110 95 20, reference by 170 23 135 20 Trimming A by 24 110 95 20, reference by 24 150 20 300 Trimming A by 24 110 95 20, reference by 21 110 95 20 Disparity in stage position after aligning to center of map frame: -0.01 -0.13 Trimming A by 22 90 155 20, reference by 21 110 95 20 Disparity in stage position after aligning to target position: 0.02 -0.02 (2nd move error 0.02 0.09) Finished aligning to item in 2 rounds Trimming A by 24 80 160 20, reference by 22 90 155 20 0.4 1.2 unbinned pixels; 3.9 3.3 nm along two shift axes; -4.8 1.3 nm on specimen axes Total_Shift = 2.553571 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.54 microns changed by -2.54 to target drift = 0.24 nm/sec Measured defocus = -0.95 microns changed by -0.05 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6993.tif 27 frames are being saved to U:\krios\20190717\PilA_6994.tif 27 frames are being saved to U:\krios\20190717\PilA_6995.tif 27 frames are being saved to U:\krios\20190717\PilA_6996.tif 27 frames are being saved to U:\krios\20190717\PilA_6997.tif 27 frames are being saved to U:\krios\20190717\PilA_6998.tif Running script 17 at item 308 (-656.22, 604.09) Remaining time to fill dewars is 27960 Dewars are NOT busy filling Trimming A by 170 21 135 20, reference by 200 21 20 270 Trimming A by 170 21 135 20, reference by 170 23 135 20 Trimming A by 170 21 135 20, reference by 24 150 20 300 Trimming A by 170 21 135 20, reference by 21 110 95 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.11 Trimming A by 95 20 125 20, reference by 170 23 135 20 Disparity in stage position after aligning to target position: 0.23 -0.07 (2nd move error 0.14 0.02) Finished aligning to item in 2 rounds Trimming A by 100 20 125 20, reference by 95 20 125 20 1.8 -0.9 unbinned pixels; 5.5 5.1 nm along two shift axes; 3.0 7.1 nm on specimen axes Total_Shift = 3.962923 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.53 microns changed by -2.63 to target drift = 0.15 nm/sec Measured defocus = -1.14 microns changed by 0.04 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_6999.tif 27 frames are being saved to U:\krios\20190717\PilA_7000.tif 27 frames are being saved to U:\krios\20190717\PilA_7001.tif 27 frames are being saved to U:\krios\20190717\PilA_7002.tif 27 frames are being saved to U:\krios\20190717\PilA_7003.tif 27 frames are being saved to U:\krios\20190717\PilA_7004.tif Running script 17 at item 309 (-661.75, 608.16) Remaining time to fill dewars is 27720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 95 24 85 20, reference by 170 23 135 20 Disparity in stage position after aligning to target position: 0.16 -0.15 (2nd move error 0.08 -0.06) Finished aligning to item in 1 rounds Trimming A by 95 20 90 20, reference by 95 24 85 20 -7.4 2.0 unbinned pixels; 17.0 23.7 nm along two shift axes; -6.2 -29.3 nm on specimen axes Total_Shift = 15.384289 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.51 microns changed by -2.71 to target drift = 0.10 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7005.tif 27 frames are being saved to U:\krios\20190717\PilA_7006.tif 27 frames are being saved to U:\krios\20190717\PilA_7007.tif 27 frames are being saved to U:\krios\20190717\PilA_7008.tif 27 frames are being saved to U:\krios\20190717\PilA_7009.tif 27 frames are being saved to U:\krios\20190717\PilA_7010.tif Running script 17 at item 310 (-663.09, 610.89) Remaining time to fill dewars is 27660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 155 20 90 20, reference by 170 23 135 20 Disparity in stage position after aligning to target position: 0.09 -0.14 (2nd move error 0.00 -0.05) Finished aligning to item in 1 rounds Trimming A by 150 20 90 30, reference by 155 20 90 20 -7.3 1.6 unbinned pixels; 15.4 24.0 nm along two shift axes; -4.6 -28.9 nm on specimen axes Total_Shift = 15.011737 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.30 microns changed by -2.60 to target drift = 0.08 nm/sec Measured defocus = -1.24 microns changed by -0.06 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7011.tif 27 frames are being saved to U:\krios\20190717\PilA_7012.tif 27 frames are being saved to U:\krios\20190717\PilA_7013.tif 27 frames are being saved to U:\krios\20190717\PilA_7014.tif 27 frames are being saved to U:\krios\20190717\PilA_7015.tif 27 frames are being saved to U:\krios\20190717\PilA_7016.tif Running script 17 at item 311 (-660.31, 605.47) Remaining time to fill dewars is 27540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 52 22 75 20, reference by 170 23 135 20 Disparity in stage position after aligning to target position: 0.21 -0.12 (2nd move error 0.13 -0.03) Finished aligning to item in 1 rounds Trimming A by 56 20 75 20, reference by 52 22 75 20 -2.8 1.6 unbinned pixels; 9.5 7.9 nm along two shift axes; -5.5 -11.4 nm on specimen axes Total_Shift = 6.484133 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.21 microns changed by -2.61 to target drift = 0.09 nm/sec Measured defocus = -1.38 microns changed by -0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7017.tif 27 frames are being saved to U:\krios\20190717\PilA_7018.tif 27 frames are being saved to U:\krios\20190717\PilA_7019.tif 27 frames are being saved to U:\krios\20190717\PilA_7020.tif 27 frames are being saved to U:\krios\20190717\PilA_7021.tif 27 frames are being saved to U:\krios\20190717\PilA_7022.tif Running script 17 at item 312 (-658.69, 602.69) Remaining time to fill dewars is 27300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 40 20 45 20, reference by 21 110 95 20 Disparity in stage position after aligning to target position: 0.07 -0.08 (2nd move error 0.07 0.03) Finished aligning to item in 1 rounds Trimming A by 22 22 65 20, reference by 40 20 45 20 -0.6 -1.0 unbinned pixels; 2.8 3.6 nm along two shift axes; 3.9 -2.1 nm on specimen axes Total_Shift = 2.278709 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.04 microns changed by -2.54 to target drift = 0.15 nm/sec Measured defocus = -1.44 microns changed by -0.06 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7023.tif 27 frames are being saved to U:\krios\20190717\PilA_7024.tif 27 frames are being saved to U:\krios\20190717\PilA_7025.tif 27 frames are being saved to U:\krios\20190717\PilA_7026.tif 27 frames are being saved to U:\krios\20190717\PilA_7027.tif 27 frames are being saved to U:\krios\20190717\PilA_7028.tif Running script 17 at item 313 (-655.85, 597.20) Remaining time to fill dewars is 27240 Dewars are NOT busy filling Trimming A by 21 105 95 20, reference by 200 21 20 270 Trimming A by 21 105 95 20, reference by 170 23 135 20 Trimming A by 21 105 95 20, reference by 24 150 20 300 Trimming A by 21 105 95 20, reference by 21 110 95 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.16 Trimming A by 24 160 160 20, reference by 21 110 95 20 Disparity in stage position after aligning to target position: 0.12 -0.01 (2nd move error 0.01 0.13) Finished aligning to item in 2 rounds Trimming A by 24 155 160 20, reference by 24 160 160 20 -0.3 0.5 unbinned pixels; 2.1 0.4 nm along two shift axes; -1.7 -1.3 nm on specimen axes Total_Shift = 1.112127 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.95 microns changed by -2.55 to target drift = 0.16 nm/sec Measured defocus = -1.57 microns changed by -0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7029.tif 27 frames are being saved to U:\krios\20190717\PilA_7030.tif 27 frames are being saved to U:\krios\20190717\PilA_7031.tif 27 frames are being saved to U:\krios\20190717\PilA_7032.tif 27 frames are being saved to U:\krios\20190717\PilA_7033.tif 27 frames are being saved to U:\krios\20190717\PilA_7034.tif Running script 17 at item 314 (-658.56, 595.79) Remaining time to fill dewars is 27000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 145 125 20, reference by 21 110 95 20 Disparity in stage position after aligning to target position: 0.19 -0.11 (2nd move error 0.09 0.03) Finished aligning to item in 1 rounds Trimming A by 22 145 125 20, reference by 22 145 125 20 0.1 0.6 unbinned pixels; 2.2 1.5 nm along two shift axes; -2.5 0.4 nm on specimen axes Total_Shift = 1.317121 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.94 microns changed by -2.64 to target drift = 0.13 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7035.tif 27 frames are being saved to U:\krios\20190717\PilA_7036.tif 27 frames are being saved to U:\krios\20190717\PilA_7037.tif 27 frames are being saved to U:\krios\20190717\PilA_7038.tif 27 frames are being saved to U:\krios\20190717\PilA_7039.tif 27 frames are being saved to U:\krios\20190717\PilA_7040.tif Running script 17 at item 315 (-659.92, 598.52) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 75 70 20, reference by 21 110 95 20 Disparity in stage position after aligning to target position: 0.09 -0.13 (2nd move error -0.02 0.01) Finished aligning to item in 1 rounds Trimming A by 24 75 70 20, reference by 24 75 70 20 1.4 3.8 unbinned pixels; 12.2 10.8 nm along two shift axes; -15.2 4.6 nm on specimen axes Total_Shift = 8.144409 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.88 microns changed by -2.68 to target drift = 0.12 nm/sec Measured defocus = -1.82 microns changed by 0.02 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7041.tif 27 frames are being saved to U:\krios\20190717\PilA_7042.tif 27 frames are being saved to U:\krios\20190717\PilA_7043.tif 27 frames are being saved to U:\krios\20190717\PilA_7044.tif 27 frames are being saved to U:\krios\20190717\PilA_7045.tif 27 frames are being saved to U:\krios\20190717\PilA_7046.tif Running script 17 at item 316 (-661.32, 601.29) Remaining time to fill dewars is 26820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 21 110 95 20 Disparity in stage position after aligning to target position: 0.01 -0.17 (2nd move error -0.10 -0.03) Finished aligning to item in 1 rounds -2.9 18.6 unbinned pixels; 72.1 17.6 nm along two shift axes; -71.7 -15.2 nm on specimen axes Total_Shift = 37.616626 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.81 microns changed by -2.71 to target drift = 0.08 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7047.tif 27 frames are being saved to U:\krios\20190717\PilA_7048.tif 27 frames are being saved to U:\krios\20190717\PilA_7049.tif 27 frames are being saved to U:\krios\20190717\PilA_7050.tif 27 frames are being saved to U:\krios\20190717\PilA_7051.tif 27 frames are being saved to U:\krios\20190717\PilA_7052.tif Running script 17 at item 317 (-662.87, 604.02) Remaining time to fill dewars is 26580 Dewars are NOT busy filling Trimming A by 175 23 130 20, reference by 200 21 20 270 Trimming A by 175 23 130 20, reference by 170 23 135 20 Trimming A by 175 23 130 20, reference by 24 150 20 300 Trimming A by 175 23 130 20, reference by 21 110 95 20 Disparity in stage position after aligning to center of map frame: 0.20 -0.11 Trimming A by 20 20 20 20, reference by 170 23 135 20 Disparity in stage position after aligning to target position: 0.25 -0.17 (2nd move error 0.04 -0.08) Finished aligning to item in 2 rounds -10.9 -1.8 unbinned pixels; 7.8 41.9 nm along two shift axes; 9.1 -42.0 nm on specimen axes Total_Shift = 22.07848 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.70 microns changed by -2.70 to target drift = 0.12 nm/sec Measured defocus = -2.05 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7053.tif 27 frames are being saved to U:\krios\20190717\PilA_7054.tif 27 frames are being saved to U:\krios\20190717\PilA_7055.tif 27 frames are being saved to U:\krios\20190717\PilA_7056.tif 27 frames are being saved to U:\krios\20190717\PilA_7057.tif 27 frames are being saved to U:\krios\20190717\PilA_7058.tif Running script 17 at item 318 (-664.38, 606.72) Remaining time to fill dewars is 26520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 46 20 20 45, reference by 170 23 135 20 Disparity in stage position after aligning to target position: 0.18 -0.19 (2nd move error -0.02 -0.10) Finished aligning to item in 1 rounds Trimming A by 50 20 20 40, reference by 46 20 20 45 -12.8 6.0 unbinned pixels; 38.9 37.0 nm along two shift axes; -20.8 -51.1 nm on specimen axes Total_Shift = 28.306213 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.52 microns changed by -2.62 to target drift = 0.06 nm/sec Measured defocus = -2.06 microns changed by -0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7059.tif 27 frames are being saved to U:\krios\20190717\PilA_7060.tif 27 frames are being saved to U:\krios\20190717\PilA_7061.tif 27 frames are being saved to U:\krios\20190717\PilA_7062.tif 27 frames are being saved to U:\krios\20190717\PilA_7063.tif 27 frames are being saved to U:\krios\20190717\PilA_7064.tif Running script 17 at item 319 (-668.45, 607.79) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Trimming A by 205 24 20 275, reference by 200 21 20 270 Trimming A by 205 24 20 275, reference by 170 23 135 20 Trimming A by 205 24 20 275, reference by 24 150 20 300 Trimming A by 205 24 20 275, reference by 21 110 95 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.07 Trimming A by 120 24 20 145, reference by 200 21 20 270 Disparity in stage position after aligning to target position: 0.21 -0.05 (2nd move error 0.12 -0.00) Finished aligning to item in 2 rounds Trimming A by 120 24 20 140, reference by 120 24 20 145 -1.1 0.8 unbinned pixels; 4.5 2.9 nm along two shift axes; -2.9 -4.6 nm on specimen axes Total_Shift = 2.806028 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.54 microns changed by -2.74 to target drift = 0.20 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7065.tif 27 frames are being saved to U:\krios\20190717\PilA_7066.tif 27 frames are being saved to U:\krios\20190717\PilA_7067.tif 27 frames are being saved to U:\krios\20190717\PilA_7068.tif 27 frames are being saved to U:\krios\20190717\PilA_7069.tif 27 frames are being saved to U:\krios\20190717\PilA_7070.tif Running script 17 at item 320 (-667.05, 605.20) Remaining time to fill dewars is 26100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 60 21 20 85, reference by 200 21 20 270 Disparity in stage position after aligning to target position: 0.32 -0.05 (2nd move error 0.22 -0.00) Finished aligning to item in 1 rounds Trimming A by 55 21 20 80, reference by 60 21 20 85 -0.3 -2.3 unbinned pixels; 8.1 4.7 nm along two shift axes; 9.1 -0.8 nm on specimen axes Total_Shift = 4.697705 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.88 microns changed by -1.42 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7071.tif 27 frames are being saved to U:\krios\20190717\PilA_7072.tif 27 frames are being saved to U:\krios\20190717\PilA_7073.tif 27 frames are being saved to U:\krios\20190717\PilA_7074.tif 27 frames are being saved to U:\krios\20190717\PilA_7075.tif 27 frames are being saved to U:\krios\20190717\PilA_7076.tif Running script 17 at item 321 (-665.37, 602.59) Remaining time to fill dewars is 25980 Dewars are NOT busy filling Trimming A by 21 105 95 20, reference by 200 21 20 270 Trimming A by 21 105 95 20, reference by 170 23 135 20 Trimming A by 21 105 95 20, reference by 24 150 20 300 Trimming A by 21 105 95 20, reference by 21 110 95 20 Disparity in stage position after aligning to center of map frame: 0.11 -0.13 Trimming A by 20 20 20 50, reference by 21 110 95 20 Disparity in stage position after aligning to target position: -0.06 -0.19 (2nd move error -0.18 -0.08) Finished aligning to item in 2 rounds Trimming A by 20 25 20 50, reference by 20 20 20 50 -10.3 32.9 unbinned pixels; 134.5 12.4 nm along two shift axes; -125.9 -47.1 nm on specimen axes Total_Shift = 68.990421 Trimming A by 20 20 20 55, reference by 20 20 20 50 -3.1 22.9 unbinned pixels; 88.3 23.2 nm along two shift axes; -88.4 -17.0 nm on specimen axes Total_Shift = 46.222208 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.33 microns changed by -2.73 to target drift = 0.04 nm/sec Measured defocus = -2.44 microns changed by 0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7077.tif 27 frames are being saved to U:\krios\20190717\PilA_7078.tif 27 frames are being saved to U:\krios\20190717\PilA_7079.tif 27 frames are being saved to U:\krios\20190717\PilA_7080.tif 27 frames are being saved to U:\krios\20190717\PilA_7081.tif 27 frames are being saved to U:\krios\20190717\PilA_7082.tif Running script 17 at item 322 (-663.92, 599.87) Remaining time to fill dewars is 25740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 40 20 45, reference by 21 110 95 20 Disparity in stage position after aligning to target position: -0.02 -0.20 (2nd move error -0.13 -0.08) Finished aligning to item in 1 rounds Trimming A by 20 30 20 55, reference by 21 40 20 45 -7.9 23.7 unbinned pixels; 97.3 7.2 nm along two shift axes; -90.4 -35.7 nm on specimen axes Total_Shift = 49.88987 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -1.13 microns changed by -1.37 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7083.tif 27 frames are being saved to U:\krios\20190717\PilA_7084.tif 27 frames are being saved to U:\krios\20190717\PilA_7085.tif 27 frames are being saved to U:\krios\20190717\PilA_7086.tif 27 frames are being saved to U:\krios\20190717\PilA_7087.tif 27 frames are being saved to U:\krios\20190717\PilA_7088.tif Running script 17 at item 323 (-662.53, 597.04) Remaining time to fill dewars is 25680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 65 40 60, reference by 21 110 95 20 Disparity in stage position after aligning to target position: 0.13 -0.19 (2nd move error 0.02 -0.08) Finished aligning to item in 1 rounds Trimming A by 21 70 35 55, reference by 20 65 40 60 -10.0 -1.4 unbinned pixels; 7.9 37.9 nm along two shift axes; 7.5 -38.4 nm on specimen axes Total_Shift = 20.099394 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.07 microns changed by -2.67 to target drift = 0.12 nm/sec Measured defocus = -2.63 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7089.tif 27 frames are being saved to U:\krios\20190717\PilA_7090.tif 27 frames are being saved to U:\krios\20190717\PilA_7091.tif 27 frames are being saved to U:\krios\20190717\PilA_7092.tif 27 frames are being saved to U:\krios\20190717\PilA_7093.tif 27 frames are being saved to U:\krios\20190717\PilA_7094.tif Running script 17 at item 324 (-661.12, 594.36) Remaining time to fill dewars is 25560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 140 90 30, reference by 21 110 95 20 Disparity in stage position after aligning to target position: 0.15 -0.13 (2nd move error 0.03 -0.01) Finished aligning to item in 1 rounds Trimming A by 24 140 90 30, reference by 24 140 90 30 -4.1 -0.7 unbinned pixels; 2.9 15.7 nm along two shift axes; 3.4 -15.8 nm on specimen axes Total_Shift = 8.304523 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 0.47 microns changed by -1.37 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7095.tif 27 frames are being saved to U:\krios\20190717\PilA_7096.tif 27 frames are being saved to U:\krios\20190717\PilA_7097.tif 27 frames are being saved to U:\krios\20190717\PilA_7098.tif 27 frames are being saved to U:\krios\20190717\PilA_7099.tif 27 frames are being saved to U:\krios\20190717\PilA_7100.tif Running script 17 at item 325 (-665.17, 595.64) Remaining time to fill dewars is 25380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 107 20 140, reference by 21 110 95 20 Disparity in stage position after aligning to target position: 0.11 -0.25 (2nd move error -0.01 -0.14) Finished aligning to item in 1 rounds Trimming A by 22 115 20 135, reference by 23 107 20 140 -15.5 3.0 unbinned pixels; 31.3 51.2 nm along two shift axes; -8.5 -60.9 nm on specimen axes Total_Shift = 31.555816 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.91 microns changed by -2.91 to target drift = 0.04 nm/sec Measured defocus = -1.05 microns changed by 0.05 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7101.tif 27 frames are being saved to U:\krios\20190717\PilA_7102.tif 27 frames are being saved to U:\krios\20190717\PilA_7103.tif 27 frames are being saved to U:\krios\20190717\PilA_7104.tif 27 frames are being saved to U:\krios\20190717\PilA_7105.tif 27 frames are being saved to U:\krios\20190717\PilA_7106.tif Running script 17 at item 326 (-666.56, 598.26) Remaining time to fill dewars is 25200 Dewars are NOT busy filling Trimming A by 21 170 20 295, reference by 200 21 20 270 Trimming A by 21 170 20 295, reference by 170 23 135 20 Trimming A by 21 170 20 295, reference by 24 150 20 300 Trimming A by 21 170 20 295, reference by 21 110 95 20 Disparity in stage position after aligning to center of map frame: 0.07 -0.11 Trimming A by 23 65 20 150, reference by 24 150 20 300 Disparity in stage position after aligning to target position: 0.18 0.02 (2nd move error 0.10 0.11) Finished aligning to item in 2 rounds Trimming A by 24 56 20 150, reference by 23 65 20 150 -0.6 2.7 unbinned pixels; 10.6 2.0 nm along two shift axes; -10.3 -2.7 nm on specimen axes Total_Shift = 5.467415 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.78 microns changed by -2.88 to target drift = 0.13 nm/sec Measured defocus = -1.09 microns changed by -0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7107.tif 27 frames are being saved to U:\krios\20190717\PilA_7108.tif 27 frames are being saved to U:\krios\20190717\PilA_7109.tif 27 frames are being saved to U:\krios\20190717\PilA_7110.tif 27 frames are being saved to U:\krios\20190717\PilA_7111.tif 27 frames are being saved to U:\krios\20190717\PilA_7112.tif Running script 17 at item 327 (-669.60, 603.69) Remaining time to fill dewars is 25080 Dewars are NOT busy filling Trimming A by 205 21 20 275, reference by 200 21 20 270 Trimming A by 205 21 20 275, reference by 170 23 135 20 Trimming A by 205 21 20 275, reference by 24 150 20 300 Trimming A by 205 21 20 275, reference by 21 110 95 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.09 Trimming A by 24 24 20 165, reference by 200 21 20 270 Disparity in stage position after aligning to target position: 0.34 -0.09 (2nd move error 0.23 -0.02) Finished aligning to item in 2 rounds Trimming A by 23 23 20 155, reference by 24 24 20 165 -1.0 3.3 unbinned pixels; 13.3 1.4 nm along two shift axes; -12.5 -4.5 nm on specimen axes Total_Shift = 6.84858 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.66 microns changed by -2.86 to target drift = 0.11 nm/sec Measured defocus = -1.20 microns changed by 0.00 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7113.tif 27 frames are being saved to U:\krios\20190717\PilA_7114.tif 27 frames are being saved to U:\krios\20190717\PilA_7115.tif 27 frames are being saved to U:\krios\20190717\PilA_7116.tif 27 frames are being saved to U:\krios\20190717\PilA_7117.tif 27 frames are being saved to U:\krios\20190717\PilA_7118.tif Running script 17 at item 328 (-667.93, 600.95) Remaining time to fill dewars is 24900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 47 20 145, reference by 24 150 20 300 Disparity in stage position after aligning to target position: 0.09 -0.06 (2nd move error 0.02 0.03) Finished aligning to item in 1 rounds Trimming A by 20 33 20 150, reference by 21 47 20 145 -1.0 -2.0 unbinned pixels; 6.2 6.7 nm along two shift axes; 8.1 -3.5 nm on specimen axes Total_Shift = 4.54874 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 0.97 microns changed by -2.27 to target drift = 0.27 nm/sec Measured defocus = -0.80 microns changed by -0.50 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7119.tif 27 frames are being saved to U:\krios\20190717\PilA_7120.tif 27 frames are being saved to U:\krios\20190717\PilA_7121.tif 27 frames are being saved to U:\krios\20190717\PilA_7122.tif 27 frames are being saved to U:\krios\20190717\PilA_7123.tif 27 frames are being saved to U:\krios\20190717\PilA_7124.tif Running script 17 at item 329 (163.02, 17.55) Remaining time to fill dewars is 24720 Dewars are NOT busy filling Trimming A by 20 155 145 20, reference by 235 21 20 235 Trimming A by 20 155 145 20, reference by 215 21 170 20 Trimming A by 20 155 145 20, reference by 22 175 20 215 Trimming A by 20 155 145 20, reference by 21 147 155 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.21 Trimming A by 21 83 175 20, reference by 21 147 155 20 Disparity in stage position after aligning to target position: -0.13 -0.16 (2nd move error -0.09 0.03) Finished aligning to item in 2 rounds Trimming A by 21 87 170 20, reference by 21 83 175 20 3.9 16.7 unbinned pixels; 56.2 39.2 nm along two shift axes; -65.7 11.7 nm on specimen axes Total_Shift = 34.259233 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.21 microns changed by -2.61 to target drift = 0.21 nm/sec Measured defocus = -1.35 microns changed by -0.05 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7125.tif 27 frames are being saved to U:\krios\20190717\PilA_7126.tif 27 frames are being saved to U:\krios\20190717\PilA_7127.tif 27 frames are being saved to U:\krios\20190717\PilA_7128.tif 27 frames are being saved to U:\krios\20190717\PilA_7129.tif 27 frames are being saved to U:\krios\20190717\PilA_7130.tif Running script 17 at item 330 (161.61, 20.36) Remaining time to fill dewars is 24600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 33 115 20, reference by 21 147 155 20 Disparity in stage position after aligning to target position: -0.12 -0.26 (2nd move error -0.08 -0.06) Finished aligning to item in 1 rounds Trimming A by 75 35 105 20, reference by 65 33 115 20 -6.9 14.8 unbinned pixels; 63.3 2.5 nm along two shift axes; -56.0 -29.9 nm on specimen axes Total_Shift = 32.604762 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.00 microns changed by -2.50 to target drift = 0.08 nm/sec Measured defocus = -1.43 microns changed by -0.07 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7131.tif 27 frames are being saved to U:\krios\20190717\PilA_7132.tif 27 frames are being saved to U:\krios\20190717\PilA_7133.tif 27 frames are being saved to U:\krios\20190717\PilA_7134.tif 27 frames are being saved to U:\krios\20190717\PilA_7135.tif 27 frames are being saved to U:\krios\20190717\PilA_7136.tif Running script 17 at item 331 (160.04, 23.01) Remaining time to fill dewars is 24360 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 220 21 170 20, reference by 235 21 20 235 Trimming A by 220 21 170 20, reference by 215 21 170 20 Trimming A by 220 21 170 20, reference by 22 175 20 215 Trimming A by 220 21 170 20, reference by 21 147 155 20 Disparity in stage position after aligning to center of map frame: -0.02 -0.14 Trimming A by 110 23 115 20, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.05 -0.21 (2nd move error 0.06 -0.09) Finished aligning to item in 2 rounds Trimming A by 110 23 115 20, reference by 110 23 115 20 -13.0 -0.6 unbinned pixels; 14.9 47.8 nm along two shift axes; 5.0 -50.6 nm on specimen axes Total_Shift = 26.116203 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.81 microns changed by -2.41 to target drift = 0.10 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7137.tif 27 frames are being saved to U:\krios\20190717\PilA_7138.tif 27 frames are being saved to U:\krios\20190717\PilA_7139.tif 27 frames are being saved to U:\krios\20190717\PilA_7140.tif 27 frames are being saved to U:\krios\20190717\PilA_7141.tif 27 frames are being saved to U:\krios\20190717\PilA_7142.tif Running script 17 at item 332 (154.66, 27.16) Remaining time to fill dewars is 24180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 170 20 35 100, reference by 215 21 170 20 Disparity in stage position after aligning to target position: -0.04 -0.27 (2nd move error -0.03 -0.15) Finished aligning to item in 1 rounds Trimming A by 170 20 30 100, reference by 170 20 35 100 -20.8 5.6 unbinned pixels; 47.7 66.6 nm along two shift axes; -17.3 -82.2 nm on specimen axes Total_Shift = 43.142167 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.60 microns changed by -2.30 to target drift = 0.02 nm/sec Measured defocus = -1.69 microns changed by -0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7143.tif 27 frames are being saved to U:\krios\20190717\PilA_7144.tif 27 frames are being saved to U:\krios\20190717\PilA_7145.tif 27 frames are being saved to U:\krios\20190717\PilA_7146.tif 27 frames are being saved to U:\krios\20190717\PilA_7147.tif 27 frames are being saved to U:\krios\20190717\PilA_7148.tif Running script 17 at item 333 (156.03, 24.38) Remaining time to fill dewars is 24060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 90 20 60 55, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.07 -0.25 (2nd move error 0.09 -0.13) Finished aligning to item in 1 rounds Trimming A by 90 20 60 50, reference by 90 20 60 55 -17.5 -0.7 unbinned pixels; 20.4 64.1 nm along two shift axes; 6.3 -68.0 nm on specimen axes Total_Shift = 35.076566 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.56 microns changed by -2.36 to target drift = 0.11 nm/sec Measured defocus = -1.77 microns changed by -0.03 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7149.tif 27 frames are being saved to U:\krios\20190717\PilA_7150.tif 27 frames are being saved to U:\krios\20190717\PilA_7151.tif 27 frames are being saved to U:\krios\20190717\PilA_7152.tif 27 frames are being saved to U:\krios\20190717\PilA_7153.tif 27 frames are being saved to U:\krios\20190717\PilA_7154.tif Running script 17 at item 334 (157.37, 21.71) Remaining time to fill dewars is 23880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 50 20 45 20, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.10 -0.25 (2nd move error 0.12 -0.13) Finished aligning to item in 1 rounds Trimming A by 50 20 45 20, reference by 50 20 45 20 -15.2 0.0 unbinned pixels; 19.8 54.6 nm along two shift axes; 3.2 -59.0 nm on specimen axes Total_Shift = 30.317045 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.54 microns changed by -2.44 to target drift = 0.11 nm/sec Measured defocus = -1.90 microns changed by 0.00 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7155.tif 27 frames are being saved to U:\krios\20190717\PilA_7156.tif 27 frames are being saved to U:\krios\20190717\PilA_7157.tif 27 frames are being saved to U:\krios\20190717\PilA_7158.tif 27 frames are being saved to U:\krios\20190717\PilA_7159.tif 27 frames are being saved to U:\krios\20190717\PilA_7160.tif Running script 17 at item 335 (159.01, 18.99) Remaining time to fill dewars is 23760 Dewars are NOT busy filling Trimming A by 22 160 140 20, reference by 235 21 20 235 Trimming A by 22 160 140 20, reference by 215 21 170 20 Trimming A by 22 160 140 20, reference by 22 175 20 215 Trimming A by 22 160 140 20, reference by 21 147 155 20 Disparity in stage position after aligning to center of map frame: -0.01 -0.15 Trimming A by 21 35 45 20, reference by 21 147 155 20 Disparity in stage position after aligning to target position: -0.07 -0.31 (2nd move error -0.06 -0.17) Finished aligning to item in 2 rounds Trimming A by 21 35 45 20, reference by 21 35 45 20 -20.3 12.1 unbinned pixels; 71.0 54.9 nm along two shift axes; -42.8 -81.6 nm on specimen axes Total_Shift = 47.291069 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.63 microns changed by -2.63 to target drift = 0.00 nm/sec Measured defocus = -2.06 microns changed by 0.06 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7161.tif 27 frames are being saved to U:\krios\20190717\PilA_7162.tif 27 frames are being saved to U:\krios\20190717\PilA_7163.tif 27 frames are being saved to U:\krios\20190717\PilA_7164.tif 27 frames are being saved to U:\krios\20190717\PilA_7165.tif 27 frames are being saved to U:\krios\20190717\PilA_7166.tif Running script 17 at item 336 (160.32, 16.18) Remaining time to fill dewars is 23580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 95 110 20, reference by 21 147 155 20 Disparity in stage position after aligning to target position: -0.10 -0.24 (2nd move error -0.09 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 95 110 20, reference by 20 95 110 20 -12.1 16.8 unbinned pixels; 77.5 18.3 nm along two shift axes; -62.7 -50.6 nm on specimen axes Total_Shift = 41.373319 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.55 microns changed by -2.65 to target drift = 0.06 nm/sec Measured defocus = -2.10 microns changed by 0.00 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7167.tif 27 frames are being saved to U:\krios\20190717\PilA_7168.tif 27 frames are being saved to U:\krios\20190717\PilA_7169.tif 27 frames are being saved to U:\krios\20190717\PilA_7170.tif 27 frames are being saved to U:\krios\20190717\PilA_7171.tif 27 frames are being saved to U:\krios\20190717\PilA_7172.tif Running script 17 at item 337 (157.70, 14.81) Remaining time to fill dewars is 23340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 90 65 20, reference by 21 147 155 20 Disparity in stage position after aligning to target position: -0.05 -0.32 (2nd move error -0.04 -0.19) Finished aligning to item in 1 rounds Trimming A by 22 90 65 20, reference by 22 90 65 20 -24.3 9.2 unbinned pixels; 65.4 73.7 nm along two shift axes; -30.5 -96.5 nm on specimen axes Total_Shift = 51.937453 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.47 microns changed by -2.67 to target drift = 0.02 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7173.tif 27 frames are being saved to U:\krios\20190717\PilA_7174.tif 27 frames are being saved to U:\krios\20190717\PilA_7175.tif 27 frames are being saved to U:\krios\20190717\PilA_7176.tif 27 frames are being saved to U:\krios\20190717\PilA_7177.tif 27 frames are being saved to U:\krios\20190717\PilA_7178.tif Running script 17 at item 338 (156.36, 17.59) Remaining time to fill dewars is 23160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 21 147 155 20 Disparity in stage position after aligning to target position: -0.07 -0.38 (2nd move error -0.06 -0.25) Finished aligning to item in 1 rounds -30.3 22.3 unbinned pixels; 121.7 75.6 nm along two shift axes; -80.5 -122.7 nm on specimen axes Total_Shift = 75.342424 -0.2 13.9 unbinned pixels; 51.2 20.2 nm along two shift axes; -53.9 -3.7 nm on specimen axes Total_Shift = 27.732002 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.33 microns changed by -2.63 to target drift = 0.02 nm/sec Measured defocus = -2.38 microns changed by 0.08 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7179.tif 27 frames are being saved to U:\krios\20190717\PilA_7180.tif 27 frames are being saved to U:\krios\20190717\PilA_7181.tif 27 frames are being saved to U:\krios\20190717\PilA_7182.tif 27 frames are being saved to U:\krios\20190717\PilA_7183.tif 27 frames are being saved to U:\krios\20190717\PilA_7184.tif Running script 17 at item 339 (154.49, 20.34) Remaining time to fill dewars is 23040 Dewars are NOT busy filling Trimming A by 215 23 170 20, reference by 235 21 20 235 Trimming A by 215 23 170 20, reference by 215 21 170 20 Trimming A by 215 23 170 20, reference by 22 175 20 215 Trimming A by 215 23 170 20, reference by 21 147 155 20 Disparity in stage position after aligning to center of map frame: 0.00 -0.05 Trimming A by 36 20 20 30, reference by 215 21 170 20 Disparity in stage position after aligning to target position: 0.10 -0.32 (2nd move error 0.09 -0.29) Finished aligning to item in 2 rounds Trimming A by 30 20 20 30, reference by 36 20 20 30 -25.6 -2.7 unbinned pixels; 23.5 96.2 nm along two shift axes; 15.9 -99.0 nm on specimen axes Total_Shift = 51.473932 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.10 microns changed by -2.50 to target drift = 0.17 nm/sec Measured defocus = -2.45 microns changed by 0.05 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7185.tif 27 frames are being saved to U:\krios\20190717\PilA_7186.tif 27 frames are being saved to U:\krios\20190717\PilA_7187.tif 27 frames are being saved to U:\krios\20190717\PilA_7188.tif 27 frames are being saved to U:\krios\20190717\PilA_7189.tif 27 frames are being saved to U:\krios\20190717\PilA_7190.tif Running script 17 at item 340 (153.24, 22.65) Remaining time to fill dewars is 22860 Dewars are NOT busy filling Trimming A by 240 24 20 235, reference by 235 21 20 235 Trimming A by 240 24 20 235, reference by 215 21 170 20 Trimming A by 240 24 20 235, reference by 22 175 20 215 Trimming A by 240 24 20 235, reference by 21 147 155 20 Disparity in stage position after aligning to center of map frame: 0.02 -0.03 Trimming A by 85 20 20 90, reference by 235 21 20 235 Disparity in stage position after aligning to target position: 0.28 0.16 (2nd move error 0.25 0.17) Finished aligning to item in 2 rounds Trimming A by 90 24 20 85, reference by 85 20 20 90 1.7 2.6 unbinned pixels; 7.2 10.0 nm along two shift axes; -10.3 6.1 nm on specimen axes Total_Shift = 6.156825 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -0.19 microns changed by -2.31 to target drift = 0.14 nm/sec Measured defocus = -2.53 microns changed by 0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7191.tif 27 frames are being saved to U:\krios\20190717\PilA_7192.tif 27 frames are being saved to U:\krios\20190717\PilA_7193.tif 27 frames are being saved to U:\krios\20190717\PilA_7194.tif 27 frames are being saved to U:\krios\20190717\PilA_7195.tif 27 frames are being saved to U:\krios\20190717\PilA_7196.tif Running script 17 at item 341 (150.60, 21.24) Remaining time to fill dewars is 22620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 100 20 20 130, reference by 235 21 20 235 Disparity in stage position after aligning to target position: 0.23 0.01 (2nd move error 0.20 0.02) Finished aligning to item in 1 rounds Trimming A by 82 23 20 140, reference by 100 20 20 130 3.4 1.8 unbinned pixels; 2.0 14.8 nm along two shift axes; -7.5 12.8 nm on specimen axes Total_Shift = 7.6112 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.29 microns changed by -2.31 to target drift = 0.12 nm/sec Measured defocus = -2.58 microns changed by -0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7197.tif 27 frames are being saved to U:\krios\20190717\PilA_7198.tif 27 frames are being saved to U:\krios\20190717\PilA_7199.tif 27 frames are being saved to U:\krios\20190717\PilA_7200.tif 27 frames are being saved to U:\krios\20190717\PilA_7201.tif 27 frames are being saved to U:\krios\20190717\PilA_7202.tif Running script 17 at item 342 (152.20, 18.48) Remaining time to fill dewars is 22380 Dewars are NOT busy filling Trimming A by 20 180 20 215, reference by 235 21 20 235 Trimming A by 20 180 20 215, reference by 215 21 170 20 Trimming A by 20 180 20 215, reference by 22 175 20 215 Trimming A by 20 180 20 215, reference by 21 147 155 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.09 Trimming A by 22 27 20 90, reference by 22 175 20 215 Disparity in stage position after aligning to target position: 0.10 0.12 (2nd move error 0.03 0.19) Finished aligning to item in 2 rounds Trimming A by 24 26 20 85, reference by 22 27 20 90 -0.8 1.1 unbinned pixels; 5.2 1.3 nm along two shift axes; -4.2 -3.5 nm on specimen axes Total_Shift = 2.79621 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 0.30 microns changed by -1.20 to target drift = 0.16 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7203.tif 27 frames are being saved to U:\krios\20190717\PilA_7204.tif 27 frames are being saved to U:\krios\20190717\PilA_7205.tif 27 frames are being saved to U:\krios\20190717\PilA_7206.tif 27 frames are being saved to U:\krios\20190717\PilA_7207.tif 27 frames are being saved to U:\krios\20190717\PilA_7208.tif Running script 17 at item 343 (153.50, 15.74) Remaining time to fill dewars is 22200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 20 85, reference by 22 175 20 215 Disparity in stage position after aligning to target position: 0.22 0.08 (2nd move error 0.16 0.14) Finished aligning to item in 1 rounds Trimming A by 20 75 20 80, reference by 20 80 20 85 2.8 3.6 unbinned pixels; 9.5 15.5 nm along two shift axes; -14.5 10.1 nm on specimen axes Total_Shift = 9.083587 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.66 microns changed by -2.66 to target drift = 0.16 nm/sec Measured defocus = -1.03 microns changed by 0.03 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7209.tif 27 frames are being saved to U:\krios\20190717\PilA_7210.tif 27 frames are being saved to U:\krios\20190717\PilA_7211.tif 27 frames are being saved to U:\krios\20190717\PilA_7212.tif 27 frames are being saved to U:\krios\20190717\PilA_7213.tif 27 frames are being saved to U:\krios\20190717\PilA_7214.tif Running script 17 at item 344 (155.08, 13.35) Remaining time to fill dewars is 22080 Dewars are NOT busy filling Trimming A by 22 160 145 20, reference by 235 21 20 235 Trimming A by 22 160 145 20, reference by 215 21 170 20 Trimming A by 22 160 145 20, reference by 22 175 20 215 Trimming A by 22 160 145 20, reference by 21 147 155 20 Disparity in stage position after aligning to center of map frame: 0.00 -0.06 Trimming A by 23 115 20 90, reference by 21 147 155 20 Disparity in stage position after aligning to target position: -0.04 -0.36 (2nd move error -0.06 -0.32) Finished aligning to item in 2 rounds Trimming A by 22 120 20 95, reference by 23 115 20 90 -33.3 12.9 unbinned pixels; 90.9 100.7 nm along two shift axes; -43.1 -132.4 nm on specimen axes Total_Shift = 71.490948 Trimming A by 22 125 20 95, reference by 23 115 20 90 -1.7 6.7 unbinned pixels; 26.7 4.0 nm along two shift axes; -25.5 -7.9 nm on specimen axes Total_Shift = 13.726139 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.66 microns changed by -2.76 to target drift = 0.03 nm/sec Measured defocus = -1.14 microns changed by 0.04 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7215.tif 27 frames are being saved to U:\krios\20190717\PilA_7216.tif 27 frames are being saved to U:\krios\20190717\PilA_7217.tif 27 frames are being saved to U:\krios\20190717\PilA_7218.tif 27 frames are being saved to U:\krios\20190717\PilA_7219.tif 27 frames are being saved to U:\krios\20190717\PilA_7220.tif Running script 17 at item 345 (149.53, 17.15) Remaining time to fill dewars is 21840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 81 20 160, reference by 22 175 20 215 Disparity in stage position after aligning to target position: 0.05 -0.04 (2nd move error -0.01 0.03) Finished aligning to item in 1 rounds Trimming A by 24 77 20 160, reference by 20 81 20 160 -1.6 23.6 unbinned pixels; 88.9 29.7 nm along two shift axes; -91.4 -11.3 nm on specimen axes Total_Shift = 47.298472 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.32 microns changed by -2.52 to target drift = 0.10 nm/sec Measured defocus = -1.16 microns changed by -0.04 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7221.tif 27 frames are being saved to U:\krios\20190717\PilA_7222.tif 27 frames are being saved to U:\krios\20190717\PilA_7223.tif 27 frames are being saved to U:\krios\20190717\PilA_7224.tif 27 frames are being saved to U:\krios\20190717\PilA_7225.tif 27 frames are being saved to U:\krios\20190717\PilA_7226.tif Running script 17 at item 346 (-206.71, 360.43) Remaining time to fill dewars is 21540 Dewars are NOT busy filling Trimming A by 20 145 145 20, reference by 260 24 20 240 Trimming A by 20 145 145 20, reference by 244 22 185 20 Trimming A by 20 145 145 20, reference by 21 156 20 215 Trimming A by 20 145 145 20, reference by 20 145 145 20 Disparity in stage position after aligning to center of map frame: -0.06 -0.15 Trimming A by 90 20 110 20, reference by 20 145 145 20 Disparity in stage position after aligning to target position: -0.16 -0.17 (2nd move error -0.11 -0.04) Finished aligning to item in 2 rounds Trimming A by 90 20 110 20, reference by 90 20 110 20 -3.9 21.2 unbinned pixels; 82.9 17.7 nm along two shift axes; -81.5 -19.7 nm on specimen axes Total_Shift = 43.052739 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.21 microns changed by -2.51 to target drift = 0.11 nm/sec Measured defocus = -1.26 microns changed by -0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7227.tif 27 frames are being saved to U:\krios\20190717\PilA_7228.tif 27 frames are being saved to U:\krios\20190717\PilA_7229.tif 27 frames are being saved to U:\krios\20190717\PilA_7230.tif 27 frames are being saved to U:\krios\20190717\PilA_7231.tif 27 frames are being saved to U:\krios\20190717\PilA_7232.tif Running script 17 at item 347 (-208.33, 363.15) Remaining time to fill dewars is 21420 Dewars are NOT busy filling Trimming A by 250 23 185 20, reference by 260 24 20 240 Trimming A by 250 23 185 20, reference by 244 22 185 20 Trimming A by 250 23 185 20, reference by 21 156 20 215 Trimming A by 250 23 185 20, reference by 20 145 145 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.09 Trimming A by 130 20 110 20, reference by 244 22 185 20 Disparity in stage position after aligning to target position: 0.15 -0.24 (2nd move error 0.19 -0.16) Finished aligning to item in 2 rounds Trimming A by 130 20 110 20, reference by 130 20 110 20 -19.2 -5.1 unbinned pixels; 6.5 76.9 nm along two shift axes; 23.8 -73.8 nm on specimen axes Total_Shift = 39.782558 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.17 microns changed by -2.57 to target drift = 0.17 nm/sec Measured defocus = -1.43 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7233.tif 27 frames are being saved to U:\krios\20190717\PilA_7234.tif 27 frames are being saved to U:\krios\20190717\PilA_7235.tif 27 frames are being saved to U:\krios\20190717\PilA_7236.tif 27 frames are being saved to U:\krios\20190717\PilA_7237.tif 27 frames are being saved to U:\krios\20190717\PilA_7238.tif Running script 17 at item 348 (-212.31, 364.46) Remaining time to fill dewars is 21180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 120 20 45 80, reference by 244 22 185 20 Disparity in stage position after aligning to target position: 0.04 -0.22 (2nd move error 0.08 -0.15) Finished aligning to item in 1 rounds Trimming A by 120 20 45 80, reference by 120 20 45 80 -19.2 -4.1 unbinned pixels; 10.2 75.2 nm along two shift axes; 19.8 -73.8 nm on specimen axes Total_Shift = 39.239837 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.12 microns changed by -2.62 to target drift = 0.14 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7239.tif 27 frames are being saved to U:\krios\20190717\PilA_7240.tif 27 frames are being saved to U:\krios\20190717\PilA_7241.tif 27 frames are being saved to U:\krios\20190717\PilA_7242.tif 27 frames are being saved to U:\krios\20190717\PilA_7243.tif 27 frames are being saved to U:\krios\20190717\PilA_7244.tif Running script 17 at item 349 (-210.89, 361.75) Remaining time to fill dewars is 21000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 20 45 20, reference by 244 22 185 20 Disparity in stage position after aligning to target position: 0.15 -0.23 (2nd move error 0.19 -0.16) Finished aligning to item in 1 rounds Trimming A by 65 20 50 20, reference by 65 20 45 20 -16.5 5.6 unbinned pixels; 42.1 50.9 nm along two shift axes; -18.3 -65.3 nm on specimen axes Total_Shift = 34.809943 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.98 microns changed by -2.58 to target drift = 0.08 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7245.tif 27 frames are being saved to U:\krios\20190717\PilA_7246.tif 27 frames are being saved to U:\krios\20190717\PilA_7247.tif 27 frames are being saved to U:\krios\20190717\PilA_7248.tif 27 frames are being saved to U:\krios\20190717\PilA_7249.tif 27 frames are being saved to U:\krios\20190717\PilA_7250.tif Running script 17 at item 350 (-209.36, 359.08) Remaining time to fill dewars is 20760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 21 30 20, reference by 20 145 145 20 Disparity in stage position after aligning to target position: -0.04 -0.24 (2nd move error 0.01 -0.11) Finished aligning to item in 1 rounds Trimming A by 35 21 30 20, reference by 35 21 30 20 -15.1 0.6 unbinned pixels; 21.8 53.5 nm along two shift axes; 0.9 -58.9 nm on specimen axes Total_Shift = 30.23303 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.87 microns changed by -2.57 to target drift = 0.10 nm/sec Measured defocus = -1.75 microns changed by 0.05 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7251.tif 27 frames are being saved to U:\krios\20190717\PilA_7252.tif 27 frames are being saved to U:\krios\20190717\PilA_7253.tif 27 frames are being saved to U:\krios\20190717\PilA_7254.tif 27 frames are being saved to U:\krios\20190717\PilA_7255.tif 27 frames are being saved to U:\krios\20190717\PilA_7256.tif Running script 17 at item 351 (-207.96, 356.25) Remaining time to fill dewars is 20580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 75 80 20, reference by 20 145 145 20 Disparity in stage position after aligning to target position: 0.15 -0.20 (2nd move error 0.20 -0.07) Finished aligning to item in 1 rounds Trimming A by 20 80 85 20, reference by 24 75 80 20 -3.2 3.9 unbinned pixels; 18.6 5.7 nm along two shift axes; -14.5 -13.4 nm on specimen axes Total_Shift = 10.146509 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.74 microns changed by -2.54 to target drift = 0.11 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7257.tif 27 frames are being saved to U:\krios\20190717\PilA_7258.tif 27 frames are being saved to U:\krios\20190717\PilA_7259.tif 27 frames are being saved to U:\krios\20190717\PilA_7260.tif 27 frames are being saved to U:\krios\20190717\PilA_7261.tif 27 frames are being saved to U:\krios\20190717\PilA_7262.tif Running script 17 at item 352 (-206.54, 353.50) Remaining time to fill dewars is 20340 Dewars are NOT busy filling Trimming A by 20 145 145 20, reference by 260 24 20 240 Trimming A by 20 145 145 20, reference by 244 22 185 20 Trimming A by 20 145 145 20, reference by 21 156 20 215 Trimming A by 20 145 145 20, reference by 20 145 145 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.09 Aligning to target with image shift equivalent to stage shift: -0.30 -0.38 Finished aligning to item in 1 rounds Trimming A by 24 137 155 20, reference by 20 145 145 20 -32.8 63.6 unbinned pixels; 276.5 22.4 nm along two shift axes; -240.3 -141.0 nm on specimen axes Total_Shift = 143.027297 Trimming A by 20 150 145 20, reference by 20 145 145 20 3.4 41.1 unbinned pixels; 146.6 74.0 nm along two shift axes; -160.5 4.5 nm on specimen axes Total_Shift = 82.420955 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.70 microns changed by -2.60 to target drift = 0.04 nm/sec Measured defocus = -1.93 microns changed by 0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7263.tif 27 frames are being saved to U:\krios\20190717\PilA_7264.tif 27 frames are being saved to U:\krios\20190717\PilA_7265.tif 27 frames are being saved to U:\krios\20190717\PilA_7266.tif 27 frames are being saved to U:\krios\20190717\PilA_7267.tif 27 frames are being saved to U:\krios\20190717\PilA_7268.tif Running script 17 at item 353 (-209.18, 352.05) Remaining time to fill dewars is 20220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 145 95 20, reference by 20 145 145 20 Disparity in stage position after aligning to target position: 0.14 -0.23 (2nd move error 0.10 -0.16) Finished aligning to item in 1 rounds Trimming A by 21 145 100 20, reference by 21 145 95 20 -16.5 0.4 unbinned pixels; 23.0 58.9 nm along two shift axes; 1.9 -64.4 nm on specimen axes Total_Shift = 33.077525 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.60 microns changed by -2.60 to target drift = 0.12 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7269.tif 27 frames are being saved to U:\krios\20190717\PilA_7270.tif 27 frames are being saved to U:\krios\20190717\PilA_7271.tif 27 frames are being saved to U:\krios\20190717\PilA_7272.tif 27 frames are being saved to U:\krios\20190717\PilA_7273.tif 27 frames are being saved to U:\krios\20190717\PilA_7274.tif Running script 17 at item 354 (-210.61, 354.88) Remaining time to fill dewars is 19980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 60 50 20, reference by 20 145 145 20 Disparity in stage position after aligning to target position: 0.07 -0.29 (2nd move error 0.03 -0.23) Finished aligning to item in 1 rounds Trimming A by 20 65 50 20, reference by 20 60 50 20 -28.1 1.8 unbinned pixels; 43.3 98.4 nm along two shift axes; -1.1 -109.6 nm on specimen axes Total_Shift = 56.268082 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.58 microns changed by -2.68 to target drift = 0.14 nm/sec Measured defocus = -2.20 microns changed by 0.10 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7275.tif 27 frames are being saved to U:\krios\20190717\PilA_7276.tif 27 frames are being saved to U:\krios\20190717\PilA_7277.tif 27 frames are being saved to U:\krios\20190717\PilA_7278.tif 27 frames are being saved to U:\krios\20190717\PilA_7279.tif 27 frames are being saved to U:\krios\20190717\PilA_7280.tif Running script 17 at item 355 (-212.01, 357.64) Remaining time to fill dewars is 19800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 145 145 20 Disparity in stage position after aligning to target position: 0.10 -0.34 (2nd move error 0.06 -0.27) Finished aligning to item in 1 rounds -25.6 -1.8 unbinned pixels; 26.9 94.7 nm along two shift axes; 12.2 -99.1 nm on specimen axes Total_Shift = 51.279758 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.44 microns changed by -2.64 to target drift = 0.17 nm/sec Measured defocus = -2.27 microns changed by 0.07 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7281.tif 27 frames are being saved to U:\krios\20190717\PilA_7282.tif 27 frames are being saved to U:\krios\20190717\PilA_7283.tif 27 frames are being saved to U:\krios\20190717\PilA_7284.tif 27 frames are being saved to U:\krios\20190717\PilA_7285.tif 27 frames are being saved to U:\krios\20190717\PilA_7286.tif Running script 17 at item 356 (-213.40, 360.02) Remaining time to fill dewars is 19560 Dewars are NOT busy filling Trimming A by 20 155 20 220, reference by 260 24 20 240 Trimming A by 20 155 20 220, reference by 244 22 185 20 Trimming A by 20 155 20 220, reference by 21 156 20 215 Trimming A by 20 155 20 220, reference by 20 145 145 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.09 Trimming A by 50 21 20 55, reference by 21 156 20 215 Disparity in stage position after aligning to target position: 0.04 0.06 (2nd move error -0.04 0.12) Finished aligning to item in 2 rounds Trimming A by 50 21 20 55, reference by 50 21 20 55 0.7 9.6 unbinned pixels; 34.3 17.1 nm along two shift axes; -37.5 0.8 nm on specimen axes Total_Shift = 19.245666 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.22 microns changed by -2.52 to target drift = 0.15 nm/sec Measured defocus = -2.29 microns changed by -0.01 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7287.tif 27 frames are being saved to U:\krios\20190717\PilA_7288.tif 27 frames are being saved to U:\krios\20190717\PilA_7289.tif 27 frames are being saved to U:\krios\20190717\PilA_7290.tif 27 frames are being saved to U:\krios\20190717\PilA_7291.tif 27 frames are being saved to U:\krios\20190717\PilA_7292.tif Running script 17 at item 357 (-215.06, 362.81) Remaining time to fill dewars is 19380 Dewars are NOT busy filling Trimming A by 265 21 20 245, reference by 260 24 20 240 Trimming A by 265 21 20 245, reference by 244 22 185 20 Trimming A by 265 21 20 245, reference by 21 156 20 215 Trimming A by 265 21 20 245, reference by 20 145 145 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.06 Trimming A by 125 21 20 115, reference by 260 24 20 240 Disparity in stage position after aligning to target position: 0.24 0.03 (2nd move error 0.12 0.06) Finished aligning to item in 2 rounds Trimming A by 125 21 20 115, reference by 125 21 20 115 -1.1 0.8 unbinned pixels; 4.4 2.9 nm along two shift axes; -2.8 -4.6 nm on specimen axes Total_Shift = 2.784889 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.07 microns changed by -2.47 to target drift = 0.15 nm/sec Measured defocus = -2.34 microns changed by -0.06 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7293.tif 27 frames are being saved to U:\krios\20190717\PilA_7294.tif 27 frames are being saved to U:\krios\20190717\PilA_7295.tif 27 frames are being saved to U:\krios\20190717\PilA_7296.tif 27 frames are being saved to U:\krios\20190717\PilA_7297.tif 27 frames are being saved to U:\krios\20190717\PilA_7298.tif Running script 17 at item 358 (-217.62, 361.39) Remaining time to fill dewars is 19140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 20 20 155, reference by 260 24 20 240 Disparity in stage position after aligning to target position: 0.23 -0.02 (2nd move error 0.11 0.01) Finished aligning to item in 1 rounds Trimming A by 116 20 20 165, reference by 130 20 20 155 2.1 -0.5 unbinned pixels; 4.7 7.0 nm along two shift axes; 1.5 8.5 nm on specimen axes Total_Shift = 4.416971 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -1.18 microns changed by -1.32 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7299.tif 27 frames are being saved to U:\krios\20190717\PilA_7300.tif 27 frames are being saved to U:\krios\20190717\PilA_7301.tif 27 frames are being saved to U:\krios\20190717\PilA_7302.tif 27 frames are being saved to U:\krios\20190717\PilA_7303.tif 27 frames are being saved to U:\krios\20190717\PilA_7304.tif Running script 17 at item 359 (-215.98, 358.64) Remaining time to fill dewars is 18960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 60 26 20 95, reference by 21 156 20 215 Disparity in stage position after aligning to target position: 0.12 -0.02 (2nd move error 0.04 0.05) Finished aligning to item in 1 rounds Trimming A by 60 21 20 95, reference by 60 26 20 95 -1.5 -1.7 unbinned pixels; 4.1 8.1 nm along two shift axes; 6.8 -5.6 nm on specimen axes Total_Shift = 4.545103 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.03 microns changed by -2.63 to target drift = 0.13 nm/sec Measured defocus = -2.67 microns changed by 0.07 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7305.tif 27 frames are being saved to U:\krios\20190717\PilA_7306.tif 27 frames are being saved to U:\krios\20190717\PilA_7307.tif 27 frames are being saved to U:\krios\20190717\PilA_7308.tif 27 frames are being saved to U:\krios\20190717\PilA_7309.tif 27 frames are being saved to U:\krios\20190717\PilA_7310.tif Running script 17 at item 360 (-214.57, 355.89) Remaining time to fill dewars is 18780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 70 20 90, reference by 21 156 20 215 Disparity in stage position after aligning to target position: 0.12 0.04 (2nd move error 0.04 0.11) Finished aligning to item in 1 rounds Trimming A by 21 65 20 90, reference by 21 70 20 90 -2.7 -0.0 unbinned pixels; 3.5 9.7 nm along two shift axes; 0.6 -10.5 nm on specimen axes Total_Shift = 5.408233 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 0.52 microns changed by -1.42 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7311.tif 27 frames are being saved to U:\krios\20190717\PilA_7312.tif 27 frames are being saved to U:\krios\20190717\PilA_7313.tif 27 frames are being saved to U:\krios\20190717\PilA_7314.tif 27 frames are being saved to U:\krios\20190717\PilA_7315.tif 27 frames are being saved to U:\krios\20190717\PilA_7316.tif Running script 17 at item 361 (-213.15, 353.09) Remaining time to fill dewars is 18540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 97 20 115, reference by 21 156 20 215 Disparity in stage position after aligning to target position: 0.16 0.07 (2nd move error 0.08 0.14) Finished aligning to item in 1 rounds Trimming A by 20 105 20 100, reference by 22 97 20 115 0.4 3.4 unbinned pixels; 12.1 6.5 nm along two shift axes; -13.4 0.7 nm on specimen axes Total_Shift = 6.900817 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.70 microns changed by -2.70 to target drift = 0.14 nm/sec Measured defocus = -0.98 microns changed by -0.02 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7317.tif 27 frames are being saved to U:\krios\20190717\PilA_7318.tif 27 frames are being saved to U:\krios\20190717\PilA_7319.tif 27 frames are being saved to U:\krios\20190717\PilA_7320.tif 27 frames are being saved to U:\krios\20190717\PilA_7321.tif 27 frames are being saved to U:\krios\20190717\PilA_7322.tif Running script 17 at item 362 (-211.85, 350.70) Remaining time to fill dewars is 18420 Dewars are NOT busy filling Trimming A by 20 145 145 20, reference by 260 24 20 240 Trimming A by 20 145 145 20, reference by 244 22 185 20 Trimming A by 20 145 145 20, reference by 21 156 20 215 Trimming A by 20 145 145 20, reference by 20 145 145 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.06 Trimming A by 20 160 25 105, reference by 20 145 145 20 Disparity in stage position after aligning to target position: 0.21 -0.29 (2nd move error 0.19 -0.25) Finished aligning to item in 2 rounds Trimming A by 20 160 35 105, reference by 20 160 25 105 -23.9 -2.6 unbinned pixels; 21.8 90.0 nm along two shift axes; 15.0 -92.5 nm on specimen axes Total_Shift = 48.108503 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.69 microns changed by -2.79 to target drift = 0.16 nm/sec Measured defocus = -1.14 microns changed by 0.04 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7323.tif 27 frames are being saved to U:\krios\20190717\PilA_7324.tif 27 frames are being saved to U:\krios\20190717\PilA_7325.tif 27 frames are being saved to U:\krios\20190717\PilA_7326.tif 27 frames are being saved to U:\krios\20190717\PilA_7327.tif 27 frames are being saved to U:\krios\20190717\PilA_7328.tif Running script 17 at item 363 (20.37, 234.94) Remaining time to fill dewars is 18120 Dewars are NOT busy filling Trimming A by 23 175 160 20, reference by 230 23 20 215 Trimming A by 23 175 160 20, reference by 210 23 175 20 Trimming A by 23 175 160 20, reference by 21 180 20 210 Trimming A by 23 175 160 20, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.15 Trimming A by 20 100 145 20, reference by 23 175 160 20 Disparity in stage position after aligning to target position: -0.26 -0.12 (2nd move error -0.18 0.02) Finished aligning to item in 2 rounds Trimming A by 21 86 155 20, reference by 20 100 145 20 7.9 32.7 unbinned pixels; 110.1 77.7 nm along two shift axes; -129.0 23.8 nm on specimen axes Total_Shift = 67.358772 Trimming A by 21 95 145 20, reference by 20 100 145 20 5.6 20.0 unbinned pixels; 66.2 50.3 nm along two shift axes; -79.0 17.6 nm on specimen axes Total_Shift = 41.550432 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.88 microns changed by -3.08 to target drift = 0.17 nm/sec Measured defocus = -1.24 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7329.tif 27 frames are being saved to U:\krios\20190717\PilA_7330.tif 27 frames are being saved to U:\krios\20190717\PilA_7331.tif 27 frames are being saved to U:\krios\20190717\PilA_7332.tif 27 frames are being saved to U:\krios\20190717\PilA_7333.tif 27 frames are being saved to U:\krios\20190717\PilA_7334.tif Running script 17 at item 364 (18.71, 237.65) Remaining time to fill dewars is 17940 Dewars are NOT busy filling Trimming A by 220 23 180 20, reference by 230 23 20 215 Trimming A by 220 23 180 20, reference by 210 23 175 20 Trimming A by 220 23 180 20, reference by 21 180 20 210 Trimming A by 220 23 180 20, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.11 Trimming A by 75 21 100 20, reference by 210 23 175 20 Disparity in stage position after aligning to target position: 0.01 -0.10 (2nd move error 0.15 -0.01) Finished aligning to item in 2 rounds Trimming A by 80 20 100 20, reference by 75 21 100 20 -1.7 -4.3 unbinned pixels; 13.7 12.5 nm along two shift axes; 17.1 -5.6 nm on specimen axes Total_Shift = 9.251235 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.63 microns changed by -2.93 to target drift = 0.13 nm/sec Measured defocus = -1.27 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7335.tif 27 frames are being saved to U:\krios\20190717\PilA_7336.tif 27 frames are being saved to U:\krios\20190717\PilA_7337.tif 27 frames are being saved to U:\krios\20190717\PilA_7338.tif 27 frames are being saved to U:\krios\20190717\PilA_7339.tif 27 frames are being saved to U:\krios\20190717\PilA_7340.tif Running script 17 at item 365 (17.33, 240.30) Remaining time to fill dewars is 17700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 115 22 110 20, reference by 210 23 175 20 Disparity in stage position after aligning to target position: -0.10 -0.17 (2nd move error 0.04 -0.07) Finished aligning to item in 1 rounds Trimming A by 115 22 110 20, reference by 115 22 110 20 -8.8 -0.5 unbinned pixels; 9.8 32.3 nm along two shift axes; 3.6 -34.1 nm on specimen axes Total_Shift = 17.581644 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.39 microns changed by -2.79 to target drift = 0.09 nm/sec Measured defocus = -1.30 microns changed by -0.10 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7341.tif 27 frames are being saved to U:\krios\20190717\PilA_7342.tif 27 frames are being saved to U:\krios\20190717\PilA_7343.tif 27 frames are being saved to U:\krios\20190717\PilA_7344.tif 27 frames are being saved to U:\krios\20190717\PilA_7345.tif 27 frames are being saved to U:\krios\20190717\PilA_7346.tif Running script 17 at item 366 (15.94, 243.05) Remaining time to fill dewars is 17520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 165 24 110 20, reference by 210 23 175 20 Disparity in stage position after aligning to target position: -0.01 -0.22 (2nd move error 0.13 -0.12) Finished aligning to item in 1 rounds Trimming A by 165 21 115 20, reference by 165 24 110 20 -14.9 -2.7 unbinned pixels; 9.4 57.6 nm along two shift axes; 13.7 -57.3 nm on specimen axes Total_Shift = 30.226823 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.32 microns changed by -2.82 to target drift = 0.11 nm/sec Measured defocus = -1.48 microns changed by -0.02 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7347.tif 27 frames are being saved to U:\krios\20190717\PilA_7348.tif 27 frames are being saved to U:\krios\20190717\PilA_7349.tif 27 frames are being saved to U:\krios\20190717\PilA_7350.tif 27 frames are being saved to U:\krios\20190717\PilA_7351.tif 27 frames are being saved to U:\krios\20190717\PilA_7352.tif Running script 17 at item 367 (14.70, 238.96) Remaining time to fill dewars is 17280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 20 40 20, reference by 210 23 175 20 Disparity in stage position after aligning to target position: -0.06 -0.24 (2nd move error 0.07 -0.15) Finished aligning to item in 1 rounds Trimming A by 55 20 40 20, reference by 55 20 40 20 -19.2 -0.4 unbinned pixels; 23.5 69.8 nm along two shift axes; 5.8 -74.6 nm on specimen axes Total_Shift = 38.414255 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.27 microns changed by -2.87 to target drift = 0.12 nm/sec Measured defocus = -1.59 microns changed by -0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7353.tif 27 frames are being saved to U:\krios\20190717\PilA_7354.tif 27 frames are being saved to U:\krios\20190717\PilA_7355.tif 27 frames are being saved to U:\krios\20190717\PilA_7356.tif 27 frames are being saved to U:\krios\20190717\PilA_7357.tif 27 frames are being saved to U:\krios\20190717\PilA_7358.tif Running script 17 at item 368 (16.30, 236.25) Remaining time to fill dewars is 17160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 30 30 20, reference by 23 175 160 20 Disparity in stage position after aligning to target position: -0.11 -0.25 (2nd move error -0.02 -0.12) Finished aligning to item in 1 rounds Trimming A by 20 20 40 20, reference by 21 30 30 20 -14.6 21.1 unbinned pixels; 96.8 20.9 nm along two shift axes; -79.2 -61.4 nm on specimen axes Total_Shift = 51.422915 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.31 microns changed by -3.01 to target drift = 0.05 nm/sec Measured defocus = -1.74 microns changed by 0.04 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7359.tif 27 frames are being saved to U:\krios\20190717\PilA_7360.tif 27 frames are being saved to U:\krios\20190717\PilA_7361.tif 27 frames are being saved to U:\krios\20190717\PilA_7362.tif 27 frames are being saved to U:\krios\20190717\PilA_7363.tif 27 frames are being saved to U:\krios\20190717\PilA_7364.tif Running script 17 at item 369 (17.67, 233.46) Remaining time to fill dewars is 16920 Dewars are NOT busy filling Trimming A by 23 175 160 20, reference by 230 23 20 215 Trimming A by 23 175 160 20, reference by 210 23 175 20 Trimming A by 23 175 160 20, reference by 21 180 20 210 Trimming A by 23 175 160 20, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.09 Trimming A by 21 77 110 20, reference by 23 175 160 20 Disparity in stage position after aligning to target position: -0.19 -0.15 (2nd move error -0.16 -0.08) Finished aligning to item in 2 rounds Trimming A by 23 81 105 20, reference by 21 77 110 20 -6.7 29.9 unbinned pixels; 118.5 20.9 nm along two shift axes; -114.7 -32.3 nm on specimen axes Total_Shift = 61.187725 Trimming A by 23 81 105 20, reference by 21 77 110 20 -1.4 20.5 unbinned pixels; 77.1 25.9 nm along two shift axes; -79.4 -9.6 nm on specimen axes Total_Shift = 41.075764 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.21 microns changed by -3.01 to target drift = 0.08 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7365.tif 27 frames are being saved to U:\krios\20190717\PilA_7366.tif 27 frames are being saved to U:\krios\20190717\PilA_7367.tif 27 frames are being saved to U:\krios\20190717\PilA_7368.tif 27 frames are being saved to U:\krios\20190717\PilA_7369.tif 27 frames are being saved to U:\krios\20190717\PilA_7370.tif Running script 17 at item 370 (16.43, 229.24) Remaining time to fill dewars is 16680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 155 105 20, reference by 23 175 160 20 Disparity in stage position after aligning to target position: 0.04 -0.21 (2nd move error 0.08 -0.14) Finished aligning to item in 1 rounds Trimming A by 24 160 110 20, reference by 21 155 105 20 -17.7 0.5 unbinned pixels; 25.0 63.1 nm along two shift axes; 1.8 -69.1 nm on specimen axes Total_Shift = 35.491904 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.18 microns changed by -3.08 to target drift = 0.12 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7371.tif 27 frames are being saved to U:\krios\20190717\PilA_7372.tif 27 frames are being saved to U:\krios\20190717\PilA_7373.tif 27 frames are being saved to U:\krios\20190717\PilA_7374.tif 27 frames are being saved to U:\krios\20190717\PilA_7375.tif 27 frames are being saved to U:\krios\20190717\PilA_7376.tif Running script 17 at item 371 (15.03, 232.04) Remaining time to fill dewars is 16500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 75 60 20, reference by 23 175 160 20 Disparity in stage position after aligning to target position: -0.10 -0.26 (2nd move error -0.07 -0.20) Finished aligning to item in 1 rounds Trimming A by 23 80 60 20, reference by 24 75 60 20 -32.7 21.4 unbinned pixels; 121.4 85.4 nm along two shift axes; -76.5 -131.6 nm on specimen axes Total_Shift = 78.141427 Trimming A by 24 80 60 20, reference by 24 75 60 20 2.0 13.0 unbinned pixels; 45.2 26.7 nm along two shift axes; -51.0 5.0 nm on specimen axes Total_Shift = 26.297996 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = -0.32 microns changed by -1.68 to target drift = 0.03 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7377.tif 27 frames are being saved to U:\krios\20190717\PilA_7378.tif 27 frames are being saved to U:\krios\20190717\PilA_7379.tif 27 frames are being saved to U:\krios\20190717\PilA_7380.tif 27 frames are being saved to U:\krios\20190717\PilA_7381.tif 27 frames are being saved to U:\krios\20190717\PilA_7382.tif Running script 17 at item 372 (13.64, 234.79) Remaining time to fill dewars is 16320 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 23 175 160 20 Disparity in stage position after aligning to target position: -0.16 -0.24 (2nd move error -0.12 -0.17) Finished aligning to item in 1 rounds -18.8 23.3 unbinned pixels; 110.2 32.5 nm along two shift axes; -86.8 -77.9 nm on specimen axes Total_Shift = 59.867553 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.87 microns changed by -2.97 to target drift = 0.00 nm/sec Measured defocus = -2.09 microns changed by -0.01 to target drift = 0.01 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7383.tif 27 frames are being saved to U:\krios\20190717\PilA_7384.tif 27 frames are being saved to U:\krios\20190717\PilA_7385.tif 27 frames are being saved to U:\krios\20190717\PilA_7386.tif 27 frames are being saved to U:\krios\20190717\PilA_7387.tif 27 frames are being saved to U:\krios\20190717\PilA_7388.tif Running script 17 at item 373 (11.94, 237.32) Remaining time to fill dewars is 16080 Dewars are NOT busy filling Trimming A by 230 23 20 220, reference by 230 23 20 215 Trimming A by 230 23 20 220, reference by 210 23 175 20 Trimming A by 230 23 20 220, reference by 21 180 20 210 Trimming A by 230 23 20 220, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: -0.00 -0.01 Trimming A by 35 21 20 45, reference by 230 23 20 215 Disparity in stage position after aligning to target position: 0.27 0.12 (2nd move error 0.27 0.11) Finished aligning to item in 2 rounds Trimming A by 35 20 20 40, reference by 35 21 20 45 -0.4 -1.9 unbinned pixels; 6.4 4.1 nm along two shift axes; 7.3 -1.0 nm on specimen axes Total_Shift = 3.786698 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = -0.70 microns changed by -1.50 to target drift = 0.14 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7389.tif 27 frames are being saved to U:\krios\20190717\PilA_7390.tif 27 frames are being saved to U:\krios\20190717\PilA_7391.tif 27 frames are being saved to U:\krios\20190717\PilA_7392.tif 27 frames are being saved to U:\krios\20190717\PilA_7393.tif 27 frames are being saved to U:\krios\20190717\PilA_7394.tif Running script 17 at item 374 (10.55, 239.95) Remaining time to fill dewars is 15900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 105 24 20 100, reference by 230 23 20 215 Disparity in stage position after aligning to target position: 0.22 0.02 (2nd move error 0.22 0.01) Finished aligning to item in 1 rounds Trimming A by 105 23 20 95, reference by 105 24 20 100 3.0 0.8 unbinned pixels; 0.9 12.0 nm along two shift axes; -3.8 11.4 nm on specimen axes Total_Shift = 6.193901 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = 0.47 microns changed by -2.77 to target drift = 0.14 nm/sec Measured defocus = -2.30 microns changed by 0.00 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7395.tif 27 frames are being saved to U:\krios\20190717\PilA_7396.tif 27 frames are being saved to U:\krios\20190717\PilA_7397.tif 27 frames are being saved to U:\krios\20190717\PilA_7398.tif 27 frames are being saved to U:\krios\20190717\PilA_7399.tif 27 frames are being saved to U:\krios\20190717\PilA_7400.tif Running script 17 at item 375 (7.91, 238.54) Remaining time to fill dewars is 15660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 93 24 20 160, reference by 230 23 20 215 Disparity in stage position after aligning to target position: 0.12 -0.06 (2nd move error 0.13 -0.07) Finished aligning to item in 1 rounds Trimming A by 101 20 20 150, reference by 93 24 20 160 -8.3 -1.6 unbinned pixels; 5.1 32.3 nm along two shift axes; 7.9 -32.0 nm on specimen axes Total_Shift = 16.936698 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = -0.80 microns changed by -1.60 to target drift = 0.11 nm/sec Measured defocus = -2.44 microns changed by 0.04 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7401.tif 27 frames are being saved to U:\krios\20190717\PilA_7402.tif 27 frames are being saved to U:\krios\20190717\PilA_7403.tif 27 frames are being saved to U:\krios\20190717\PilA_7404.tif 27 frames are being saved to U:\krios\20190717\PilA_7405.tif 27 frames are being saved to U:\krios\20190717\PilA_7406.tif Running script 17 at item 376 (9.56, 235.74) Remaining time to fill dewars is 15480 Dewars are NOT busy filling Trimming A by 22 171 20 220, reference by 230 23 20 215 Trimming A by 22 171 20 220, reference by 210 23 175 20 Trimming A by 22 171 20 220, reference by 21 180 20 210 Trimming A by 22 171 20 220, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: 0.07 -0.09 Trimming A by 32 32 20 90, reference by 21 180 20 210 Disparity in stage position after aligning to target position: 0.03 0.12 (2nd move error -0.04 0.19) Finished aligning to item in 2 rounds Trimming A by 50 46 20 75, reference by 32 32 20 90 -1.7 9.5 unbinned pixels; 37.3 8.1 nm along two shift axes; -36.7 -8.7 nm on specimen axes Total_Shift = 19.351019 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = 0.35 microns changed by -2.85 to target drift = 0.15 nm/sec Measured defocus = -2.48 microns changed by -0.02 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7407.tif 27 frames are being saved to U:\krios\20190717\PilA_7408.tif 27 frames are being saved to U:\krios\20190717\PilA_7409.tif 27 frames are being saved to U:\krios\20190717\PilA_7410.tif 27 frames are being saved to U:\krios\20190717\PilA_7411.tif 27 frames are being saved to U:\krios\20190717\PilA_7412.tif Running script 17 at item 377 (10.94, 232.98) Remaining time to fill dewars is 15300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 20 85, reference by 21 180 20 210 Disparity in stage position after aligning to target position: 0.02 0.09 (2nd move error -0.06 0.16) Finished aligning to item in 1 rounds Trimming A by 20 75 20 85, reference by 20 80 20 85 2.8 12.6 unbinned pixels; 42.6 29.2 nm along two shift axes; -49.6 8.4 nm on specimen axes Total_Shift = 25.82489 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -1.03 microns changed by -1.57 to target drift = 0.13 nm/sec Measured defocus = -2.54 microns changed by -0.06 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7413.tif 27 frames are being saved to U:\krios\20190717\PilA_7414.tif 27 frames are being saved to U:\krios\20190717\PilA_7415.tif 27 frames are being saved to U:\krios\20190717\PilA_7416.tif 27 frames are being saved to U:\krios\20190717\PilA_7417.tif 27 frames are being saved to U:\krios\20190717\PilA_7418.tif Running script 17 at item 378 (12.28, 230.30) Remaining time to fill dewars is 15120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 120 20 105, reference by 21 180 20 210 Disparity in stage position after aligning to target position: 0.12 0.11 (2nd move error 0.04 0.18) Finished aligning to item in 1 rounds Trimming A by 22 120 20 95, reference by 23 120 20 105 2.2 -0.5 unbinned pixels; 4.8 7.2 nm along two shift axes; 1.6 8.8 nm on specimen axes Total_Shift = 4.570469 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 2.12 microns changed by -3.02 to target drift = 0.16 nm/sec Measured defocus = -0.81 microns changed by -0.09 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7419.tif 27 frames are being saved to U:\krios\20190717\PilA_7420.tif 27 frames are being saved to U:\krios\20190717\PilA_7421.tif 27 frames are being saved to U:\krios\20190717\PilA_7422.tif 27 frames are being saved to U:\krios\20190717\PilA_7423.tif 27 frames are being saved to U:\krios\20190717\PilA_7424.tif Running script 17 at item 379 (13.78, 227.86) Remaining time to fill dewars is 14880 Dewars are NOT busy filling Trimming A by 21 180 160 20, reference by 230 23 20 215 Trimming A by 21 180 160 20, reference by 210 23 175 20 Trimming A by 21 180 160 20, reference by 21 180 20 210 Trimming A by 21 180 160 20, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.02 Trimming A by 20 170 45 100, reference by 23 175 160 20 Disparity in stage position after aligning to target position: -0.03 -0.23 (2nd move error 0.01 -0.22) Finished aligning to item in 2 rounds Trimming A by 20 170 50 105, reference by 20 170 45 100 -26.3 1.0 unbinned pixels; 37.9 93.3 nm along two shift axes; 1.8 -102.6 nm on specimen axes Total_Shift = 52.669254 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 2.25 microns changed by -3.25 to target drift = 0.09 nm/sec Measured defocus = -1.07 microns changed by 0.07 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7425.tif 27 frames are being saved to U:\krios\20190717\PilA_7426.tif 27 frames are being saved to U:\krios\20190717\PilA_7427.tif 27 frames are being saved to U:\krios\20190717\PilA_7428.tif 27 frames are being saved to U:\krios\20190717\PilA_7429.tif 27 frames are being saved to U:\krios\20190717\PilA_7430.tif Running script 17 at item 380 (-307.33, 532.39) Remaining time to fill dewars is 14700 Dewars are NOT busy filling Trimming A by 20 122 140 20, reference by 180 21 60 165 Trimming A by 20 122 140 20, reference by 185 24 155 20 Trimming A by 20 122 140 20, reference by 20 166 20 215 Trimming A by 20 122 140 20, reference by 21 126 135 20 Disparity in stage position after aligning to center of map frame: -0.12 -0.16 Trimming A by 21 25 30 20, reference by 21 126 135 20 Disparity in stage position after aligning to target position: -0.13 -0.20 (2nd move error -0.02 -0.06) Finished aligning to item in 2 rounds Trimming A by 21 25 30 20, reference by 21 25 30 20 -8.3 8.3 unbinned pixels; 41.3 17.3 nm along two shift axes; -30.5 -34.0 nm on specimen axes Total_Shift = 23.444736 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.39 microns changed by -2.49 to target drift = 0.14 nm/sec Measured defocus = -1.11 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7431.tif 27 frames are being saved to U:\krios\20190717\PilA_7432.tif 27 frames are being saved to U:\krios\20190717\PilA_7433.tif 27 frames are being saved to U:\krios\20190717\PilA_7434.tif 27 frames are being saved to U:\krios\20190717\PilA_7435.tif 27 frames are being saved to U:\krios\20190717\PilA_7436.tif Running script 17 at item 381 (-309.99, 530.95) Remaining time to fill dewars is 14400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 21 126 135 20 Disparity in stage position after aligning to target position: -0.10 -0.24 (2nd move error 0.01 -0.10) Finished aligning to item in 1 rounds -14.2 -0.4 unbinned pixels; 17.2 51.6 nm along two shift axes; 4.4 -55.1 nm on specimen axes Total_Shift = 28.382204 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.34 microns changed by -2.54 to target drift = 0.06 nm/sec Measured defocus = -1.19 microns changed by -0.01 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7437.tif 27 frames are being saved to U:\krios\20190717\PilA_7438.tif 27 frames are being saved to U:\krios\20190717\PilA_7439.tif 27 frames are being saved to U:\krios\20190717\PilA_7440.tif 27 frames are being saved to U:\krios\20190717\PilA_7441.tif 27 frames are being saved to U:\krios\20190717\PilA_7442.tif Running script 17 at item 382 (-312.53, 529.31) Remaining time to fill dewars is 14220 Dewars are NOT busy filling Trimming A by 20 175 20 210, reference by 180 21 60 165 Trimming A by 20 175 20 210, reference by 185 24 155 20 Trimming A by 20 175 20 210, reference by 20 166 20 215 Trimming A by 20 175 20 210, reference by 21 126 135 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.05 Trimming A by 21 70 20 90, reference by 20 166 20 215 Disparity in stage position after aligning to target position: -0.04 0.06 (2nd move error -0.00 0.10) Finished aligning to item in 2 rounds Trimming A by 21 70 20 90, reference by 21 70 20 90 0.4 2.9 unbinned pixels; 10.0 5.8 nm along two shift axes; -11.2 1.1 nm on specimen axes Total_Shift = 5.785446 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.23 microns changed by -2.53 to target drift = 0.13 nm/sec Measured defocus = -1.25 microns changed by -0.04 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7443.tif 27 frames are being saved to U:\krios\20190717\PilA_7444.tif 27 frames are being saved to U:\krios\20190717\PilA_7445.tif 27 frames are being saved to U:\krios\20190717\PilA_7446.tif 27 frames are being saved to U:\krios\20190717\PilA_7447.tif 27 frames are being saved to U:\krios\20190717\PilA_7448.tif Running script 17 at item 383 (-311.13, 526.69) Remaining time to fill dewars is 14100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 115 20 100, reference by 20 166 20 215 Disparity in stage position after aligning to target position: 0.06 0.03 (2nd move error 0.10 0.06) Finished aligning to item in 1 rounds Trimming A by 24 110 20 100, reference by 23 115 20 100 -0.2 2.6 unbinned pixels; 10.1 3.1 nm along two shift axes; -10.3 -1.5 nm on specimen axes Total_Shift = 5.319947 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.25 microns changed by -2.65 to target drift = 0.13 nm/sec Measured defocus = -1.42 microns changed by 0.02 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7449.tif 27 frames are being saved to U:\krios\20190717\PilA_7450.tif 27 frames are being saved to U:\krios\20190717\PilA_7451.tif 27 frames are being saved to U:\krios\20190717\PilA_7452.tif 27 frames are being saved to U:\krios\20190717\PilA_7453.tif 27 frames are being saved to U:\krios\20190717\PilA_7454.tif Running script 17 at item 384 (-308.57, 528.19) Remaining time to fill dewars is 13920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 75 55 20, reference by 21 126 135 20 Disparity in stage position after aligning to target position: 0.01 -0.22 (2nd move error 0.12 -0.08) Finished aligning to item in 1 rounds Trimming A by 24 75 60 20, reference by 24 75 55 20 -8.8 -1.0 unbinned pixels; 7.9 33.3 nm along two shift axes; 5.7 -34.2 nm on specimen axes Total_Shift = 17.794858 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.04 microns changed by -2.54 to target drift = 0.11 nm/sec Measured defocus = -1.52 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7455.tif 27 frames are being saved to U:\krios\20190717\PilA_7456.tif 27 frames are being saved to U:\krios\20190717\PilA_7457.tif 27 frames are being saved to U:\krios\20190717\PilA_7458.tif 27 frames are being saved to U:\krios\20190717\PilA_7459.tif 27 frames are being saved to U:\krios\20190717\PilA_7460.tif Running script 17 at item 385 (-307.15, 525.47) Remaining time to fill dewars is 13680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 141 110 20, reference by 21 126 135 20 Disparity in stage position after aligning to target position: 0.12 -0.16 (2nd move error 0.23 -0.02) Finished aligning to item in 1 rounds Trimming A by 21 155 105 20, reference by 21 141 110 20 -0.2 3.4 unbinned pixels; 12.8 4.5 nm along two shift axes; -13.3 -1.4 nm on specimen axes Total_Shift = 6.857313 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 0.98 microns changed by -2.58 to target drift = 0.08 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7461.tif 27 frames are being saved to U:\krios\20190717\PilA_7462.tif 27 frames are being saved to U:\krios\20190717\PilA_7463.tif 27 frames are being saved to U:\krios\20190717\PilA_7464.tif 27 frames are being saved to U:\krios\20190717\PilA_7465.tif 27 frames are being saved to U:\krios\20190717\PilA_7466.tif Running script 17 at item 386 (206.23, -21.02) Remaining time to fill dewars is 13500 Dewars are NOT busy filling Trimming A by 221 24 160 30, reference by 237 23 20 250 Trimming A by 221 24 160 30, reference by 215 22 160 20 Trimming A by 221 24 160 30, reference by 22 180 20 225 Trimming A by 221 24 160 30, reference by 22 160 140 20 Disparity in stage position after aligning to center of map frame: 0.02 -0.15 Trimming A by 90 23 95 20, reference by 215 22 160 20 Disparity in stage position after aligning to target position: 0.10 -0.21 (2nd move error 0.07 -0.09) Finished aligning to item in 2 rounds Trimming A by 80 22 105 20, reference by 90 23 95 20 -12.0 -1.9 unbinned pixels; 8.8 46.1 nm along two shift axes; 9.8 -46.4 nm on specimen axes Total_Shift = 24.326989 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 0.99 microns changed by -2.69 to target drift = 0.15 nm/sec Measured defocus = -1.70 microns changed by 0.00 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7467.tif 27 frames are being saved to U:\krios\20190717\PilA_7468.tif 27 frames are being saved to U:\krios\20190717\PilA_7469.tif 27 frames are being saved to U:\krios\20190717\PilA_7470.tif 27 frames are being saved to U:\krios\20190717\PilA_7471.tif 27 frames are being saved to U:\krios\20190717\PilA_7472.tif Running script 17 at item 387 (204.86, -18.31) Remaining time to fill dewars is 13260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 24 105 20, reference by 215 22 160 20 Disparity in stage position after aligning to target position: 0.15 -0.27 (2nd move error 0.12 -0.14) Finished aligning to item in 1 rounds Trimming A by 130 24 105 20, reference by 130 24 105 20 -16.7 -1.1 unbinned pixels; 17.8 61.6 nm along two shift axes; 7.7 -64.6 nm on specimen axes Total_Shift = 33.392136 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 0.76 microns changed by -2.56 to target drift = 0.10 nm/sec Measured defocus = -1.77 microns changed by -0.03 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7473.tif 27 frames are being saved to U:\krios\20190717\PilA_7474.tif 27 frames are being saved to U:\krios\20190717\PilA_7475.tif 27 frames are being saved to U:\krios\20190717\PilA_7476.tif 27 frames are being saved to U:\krios\20190717\PilA_7477.tif 27 frames are being saved to U:\krios\20190717\PilA_7478.tif Running script 17 at item 388 (200.87, -16.94) Remaining time to fill dewars is 13020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 20 35 90, reference by 215 22 160 20 Disparity in stage position after aligning to target position: 0.05 -0.39 (2nd move error 0.02 -0.27) Finished aligning to item in 1 rounds Trimming A by 130 20 30 80, reference by 130 20 35 90 -31.7 -0.2 unbinned pixels; 40.5 114.6 nm along two shift axes; 7.6 -123.4 nm on specimen axes Total_Shift = 63.450635 Trimming A by 125 23 30 85, reference by 130 20 35 90 -2.2 -1.7 unbinned pixels; 3.4 10.4 nm along two shift axes; 7.1 -8.1 nm on specimen axes Total_Shift = 5.517073 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 0.70 microns changed by -2.60 to target drift = 0.06 nm/sec Measured defocus = -1.92 microns changed by 0.02 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7479.tif 27 frames are being saved to U:\krios\20190717\PilA_7480.tif 27 frames are being saved to U:\krios\20190717\PilA_7481.tif 27 frames are being saved to U:\krios\20190717\PilA_7482.tif 27 frames are being saved to U:\krios\20190717\PilA_7483.tif 27 frames are being saved to U:\krios\20190717\PilA_7484.tif Running script 17 at item 389 (202.23, -19.66) Remaining time to fill dewars is 12840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 20 45 25, reference by 215 22 160 20 Disparity in stage position after aligning to target position: 0.15 -0.36 (2nd move error 0.12 -0.23) Finished aligning to item in 1 rounds Trimming A by 60 20 45 20, reference by 65 20 45 25 -26.2 0.1 unbinned pixels; 34.7 94.1 nm along two shift axes; 5.0 -102.0 nm on specimen axes Total_Shift = 52.409159 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.69 microns changed by -2.69 to target drift = 0.16 nm/sec Measured defocus = -2.05 microns changed by 0.05 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7485.tif 27 frames are being saved to U:\krios\20190717\PilA_7486.tif 27 frames are being saved to U:\krios\20190717\PilA_7487.tif 27 frames are being saved to U:\krios\20190717\PilA_7488.tif 27 frames are being saved to U:\krios\20190717\PilA_7489.tif 27 frames are being saved to U:\krios\20190717\PilA_7490.tif Running script 17 at item 390 (203.79, -22.37) Remaining time to fill dewars is 12660 Dewars are NOT busy filling Trimming A by 24 155 140 20, reference by 237 23 20 250 Trimming A by 24 155 140 20, reference by 215 22 160 20 Trimming A by 24 155 140 20, reference by 22 180 20 225 Trimming A by 24 155 140 20, reference by 22 160 140 20 Disparity in stage position after aligning to center of map frame: 0.07 -0.15 Trimming A by 35 21 30 20, reference by 22 160 140 20 Disparity in stage position after aligning to target position: -0.07 -0.31 (2nd move error -0.14 -0.18) Finished aligning to item in 2 rounds Trimming A by 30 20 30 20, reference by 35 21 30 20 -22.1 30.2 unbinned pixels; 140.0 34.0 nm along two shift axes; -112.9 -92.3 nm on specimen axes Total_Shift = 74.859338 Trimming A by 30 20 25 20, reference by 35 21 30 20 -3.9 19.5 unbinned pixels; 76.7 15.2 nm along two shift axes; -74.9 -19.3 nm on specimen axes Total_Shift = 39.699291 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.75 microns changed by -2.85 to target drift = 0.01 nm/sec Measured defocus = -2.19 microns changed by 0.09 to target drift = 0.04 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7491.tif 27 frames are being saved to U:\krios\20190717\PilA_7492.tif 27 frames are being saved to U:\krios\20190717\PilA_7493.tif 27 frames are being saved to U:\krios\20190717\PilA_7494.tif 27 frames are being saved to U:\krios\20190717\PilA_7495.tif 27 frames are being saved to U:\krios\20190717\PilA_7496.tif Running script 17 at item 391 (206.50, -27.93) Remaining time to fill dewars is 12420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 140 125 20, reference by 22 160 140 20 Disparity in stage position after aligning to target position: 0.06 -0.21 (2nd move error -0.01 -0.07) Finished aligning to item in 1 rounds Trimming A by 24 140 125 20, reference by 24 140 125 20 -9.2 2.2 unbinned pixels; 20.2 29.7 nm along two shift axes; -6.8 -36.2 nm on specimen axes Total_Shift = 18.924048 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.75 microns changed by -2.95 to target drift = 0.08 nm/sec Measured defocus = -2.20 microns changed by 0.00 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7497.tif 27 frames are being saved to U:\krios\20190717\PilA_7498.tif 27 frames are being saved to U:\krios\20190717\PilA_7499.tif 27 frames are being saved to U:\krios\20190717\PilA_7500.tif 27 frames are being saved to U:\krios\20190717\PilA_7501.tif 27 frames are being saved to U:\krios\20190717\PilA_7502.tif Running script 17 at item 392 (203.88, -29.25) Remaining time to fill dewars is 12240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 125 85 20, reference by 22 160 140 20 Disparity in stage position after aligning to target position: 0.09 -0.30 (2nd move error 0.02 -0.17) Finished aligning to item in 1 rounds Trimming A by 21 130 85 20, reference by 23 125 85 20 -24.4 0.1 unbinned pixels; 32.1 87.8 nm along two shift axes; 4.9 -95.0 nm on specimen axes Total_Shift = 48.849848 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.57 microns changed by -1.73 to target drift = 0.14 nm/sec Measured defocus = -2.32 microns changed by 0.02 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7503.tif 27 frames are being saved to U:\krios\20190717\PilA_7504.tif 27 frames are being saved to U:\krios\20190717\PilA_7505.tif 27 frames are being saved to U:\krios\20190717\PilA_7506.tif 27 frames are being saved to U:\krios\20190717\PilA_7507.tif 27 frames are being saved to U:\krios\20190717\PilA_7508.tif Running script 17 at item 393 (201.14, -23.68) Remaining time to fill dewars is 12000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 22 160 140 20 Disparity in stage position after aligning to target position: -0.02 -0.41 (2nd move error -0.10 -0.28) Finished aligning to item in 1 rounds -28.4 18.8 unbinned pixels; 106.2 73.8 nm along two shift axes; -67.2 -114.3 nm on specimen axes Total_Shift = 68.074592 -1.6 11.6 unbinned pixels; 44.7 11.8 nm along two shift axes; -44.8 -8.6 nm on specimen axes Total_Shift = 23.410678 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.40 microns changed by -2.80 to target drift = 0.04 nm/sec Measured defocus = -2.47 microns changed by 0.07 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7509.tif 27 frames are being saved to U:\krios\20190717\PilA_7510.tif 27 frames are being saved to U:\krios\20190717\PilA_7511.tif 27 frames are being saved to U:\krios\20190717\PilA_7512.tif 27 frames are being saved to U:\krios\20190717\PilA_7513.tif 27 frames are being saved to U:\krios\20190717\PilA_7514.tif Running script 17 at item 394 (195.55, -20.04) Remaining time to fill dewars is 11820 Dewars are NOT busy filling Trimming A by 245 22 20 250, reference by 237 23 20 250 Trimming A by 245 22 20 250, reference by 215 22 160 20 Trimming A by 245 22 20 250, reference by 22 180 20 225 Trimming A by 245 22 20 250, reference by 22 160 140 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.09 Trimming A by 130 20 20 165, reference by 237 23 20 250 Disparity in stage position after aligning to target position: 0.26 -0.06 (2nd move error 0.16 0.01) Finished aligning to item in 2 rounds Trimming A by 125 22 20 165, reference by 130 20 20 165 1.7 -3.3 unbinned pixels; 14.5 1.1 nm along two shift axes; 12.7 7.4 nm on specimen axes Total_Shift = 7.519347 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -1.16 microns changed by -1.34 to target drift = 0.13 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7515.tif 27 frames are being saved to U:\krios\20190717\PilA_7516.tif 27 frames are being saved to U:\krios\20190717\PilA_7517.tif 27 frames are being saved to U:\krios\20190717\PilA_7518.tif 27 frames are being saved to U:\krios\20190717\PilA_7519.tif 27 frames are being saved to U:\krios\20190717\PilA_7520.tif Running script 17 at item 395 (197.11, -22.85) Remaining time to fill dewars is 11640 Dewars are NOT busy filling Trimming A by 23 171 20 235, reference by 237 23 20 250 Trimming A by 23 171 20 235, reference by 215 22 160 20 Trimming A by 23 171 20 235, reference by 22 180 20 225 Trimming A by 23 171 20 235, reference by 22 160 140 20 Disparity in stage position after aligning to center of map frame: 0.13 -0.14 Trimming A by 65 21 20 100, reference by 22 180 20 225 Disparity in stage position after aligning to target position: 0.09 0.02 (2nd move error -0.04 0.13) Finished aligning to item in 2 rounds Trimming A by 65 21 20 100, reference by 65 21 20 100 1.6 10.1 unbinned pixels; 35.0 20.9 nm along two shift axes; -39.6 4.1 nm on specimen axes Total_Shift = 20.418498 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = 0.12 microns changed by -2.72 to target drift = 0.14 nm/sec Measured defocus = -2.65 microns changed by 0.05 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7521.tif 27 frames are being saved to U:\krios\20190717\PilA_7522.tif 27 frames are being saved to U:\krios\20190717\PilA_7523.tif 27 frames are being saved to U:\krios\20190717\PilA_7524.tif 27 frames are being saved to U:\krios\20190717\PilA_7525.tif 27 frames are being saved to U:\krios\20190717\PilA_7526.tif Running script 17 at item 396 (201.21, -30.66) Remaining time to fill dewars is 11460 Dewars are NOT busy filling Trimming A by 20 160 135 20, reference by 237 23 20 250 Trimming A by 20 160 135 20, reference by 215 22 160 20 Trimming A by 20 160 135 20, reference by 22 180 20 225 Trimming A by 20 160 135 20, reference by 22 160 140 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.12 Trimming A by 24 150 20 110, reference by 22 160 140 20 Disparity in stage position after aligning to target position: 0.08 -0.38 (2nd move error -0.02 -0.28) Finished aligning to item in 2 rounds Trimming A by 24 155 20 110, reference by 24 150 20 110 -31.2 6.3 unbinned pixels; 63.9 103.0 nm along two shift axes; -17.9 -122.9 nm on specimen axes Total_Shift = 63.747627 Trimming A by 22 160 20 110, reference by 24 150 20 110 -2.6 3.0 unbinned pixels; 14.4 4.7 nm along two shift axes; -11.2 -10.6 nm on specimen axes Total_Shift = 7.909467 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 0.96 microns changed by -1.86 to target drift = 0.03 nm/sec Measured defocus = -0.94 microns changed by 0.04 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7527.tif 27 frames are being saved to U:\krios\20190717\PilA_7528.tif 27 frames are being saved to U:\krios\20190717\PilA_7529.tif 27 frames are being saved to U:\krios\20190717\PilA_7530.tif 27 frames are being saved to U:\krios\20190717\PilA_7531.tif 27 frames are being saved to U:\krios\20190717\PilA_7532.tif Running script 17 at item 397 (197.21, -29.70) Remaining time to fill dewars is 11280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 152 20 175, reference by 22 180 20 225 Disparity in stage position after aligning to target position: 0.14 -0.05 (2nd move error 0.01 0.07) Finished aligning to item in 1 rounds Trimming A by 22 160 20 165, reference by 21 152 20 175 -0.0 -0.7 unbinned pixels; 2.7 1.2 nm along two shift axes; 2.9 0.0 nm on specimen axes Total_Shift = 1.48648 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.95 microns changed by -2.95 to target drift = 0.16 nm/sec Measured defocus = -0.90 microns changed by -0.10 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7533.tif 27 frames are being saved to U:\krios\20190717\PilA_7534.tif 27 frames are being saved to U:\krios\20190717\PilA_7535.tif 27 frames are being saved to U:\krios\20190717\PilA_7536.tif 27 frames are being saved to U:\krios\20190717\PilA_7537.tif 27 frames are being saved to U:\krios\20190717\PilA_7538.tif Running script 17 at item 398 (195.84, -26.93) Remaining time to fill dewars is 11100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 106 20 170, reference by 22 180 20 225 Disparity in stage position after aligning to target position: 0.09 -0.10 (2nd move error -0.04 0.02) Finished aligning to item in 1 rounds Trimming A by 23 110 20 165, reference by 21 106 20 170 0.7 11.0 unbinned pixels; 39.6 19.0 nm along two shift axes; -42.9 0.3 nm on specimen axes Total_Shift = 22.046779 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.86 microns changed by -2.96 to target drift = 0.15 nm/sec Measured defocus = -1.11 microns changed by 0.01 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7539.tif 27 frames are being saved to U:\krios\20190717\PilA_7540.tif 27 frames are being saved to U:\krios\20190717\PilA_7541.tif 27 frames are being saved to U:\krios\20190717\PilA_7542.tif 27 frames are being saved to U:\krios\20190717\PilA_7543.tif 27 frames are being saved to U:\krios\20190717\PilA_7544.tif Running script 17 at item 399 (78.89, -74.07) Remaining time to fill dewars is 10860 Dewars are NOT busy filling Trimming A by 245 21 190 20, reference by 250 23 20 255 Trimming A by 245 21 190 20, reference by 240 21 185 20 Trimming A by 245 21 190 20, reference by 23 187 20 250 Trimming A by 245 21 190 20, reference by 20 181 180 20 Disparity in stage position after aligning to center of map frame: -0.00 -0.12 Trimming A by 82 23 145 20, reference by 240 21 185 20 Disparity in stage position after aligning to target position: 0.06 -0.24 (2nd move error 0.06 -0.14) Finished aligning to item in 2 rounds Trimming A by 82 23 145 20, reference by 82 23 145 20 -17.0 -1.5 unbinned pixels; 16.9 63.5 nm along two shift axes; 9.3 -66.0 nm on specimen axes Total_Shift = 34.197036 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.74 microns changed by -2.94 to target drift = 0.13 nm/sec Measured defocus = -1.25 microns changed by 0.05 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7545.tif 27 frames are being saved to U:\krios\20190717\PilA_7546.tif 27 frames are being saved to U:\krios\20190717\PilA_7547.tif 27 frames are being saved to U:\krios\20190717\PilA_7548.tif 27 frames are being saved to U:\krios\20190717\PilA_7549.tif 27 frames are being saved to U:\krios\20190717\PilA_7550.tif Running script 17 at item 400 (77.43, -71.37) Remaining time to fill dewars is 10680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 145 20 130 20, reference by 240 21 185 20 Disparity in stage position after aligning to target position: 0.09 -0.20 (2nd move error 0.08 -0.11) Finished aligning to item in 1 rounds Trimming A by 145 20 130 20, reference by 145 20 130 20 -12.5 -1.6 unbinned pixels; 10.5 47.5 nm along two shift axes; 8.8 -48.4 nm on specimen axes Total_Shift = 25.247624 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = 1.53 microns changed by -2.83 to target drift = 0.11 nm/sec Measured defocus = -1.34 microns changed by 0.05 to target drift = 0.09 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7551.tif 27 frames are being saved to U:\krios\20190717\PilA_7552.tif 27 frames are being saved to U:\krios\20190717\PilA_7553.tif 27 frames are being saved to U:\krios\20190717\PilA_7554.tif 27 frames are being saved to U:\krios\20190717\PilA_7555.tif 27 frames are being saved to U:\krios\20190717\PilA_7556.tif Running script 17 at item 401 (73.42, -69.96) Remaining time to fill dewars is 10440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 140 20 60 85, reference by 240 21 185 20 Disparity in stage position after aligning to target position: 0.09 -0.30 (2nd move error 0.09 -0.21) Finished aligning to item in 1 rounds Trimming A by 144 20 50 85, reference by 140 20 60 85 -25.7 0.9 unbinned pixels; 37.0 91.1 nm along two shift axes; 1.8 -100.2 nm on specimen axes Total_Shift = 51.447932 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Measured defocus = 1.27 microns changed by -2.67 to target drift = 0.12 nm/sec Measured defocus = -1.36 microns changed by -0.04 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7557.tif 27 frames are being saved to U:\krios\20190717\PilA_7558.tif 27 frames are being saved to U:\krios\20190717\PilA_7559.tif 27 frames are being saved to U:\krios\20190717\PilA_7560.tif 27 frames are being saved to U:\krios\20190717\PilA_7561.tif 27 frames are being saved to U:\krios\20190717\PilA_7562.tif Running script 17 at item 402 (74.82, -72.76) Remaining time to fill dewars is 10320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 85 23 60 20, reference by 240 21 185 20 Disparity in stage position after aligning to target position: 0.09 -0.24 (2nd move error 0.09 -0.15) Finished aligning to item in 1 rounds Trimming A by 85 23 60 20, reference by 85 23 60 20 -17.5 0.2 unbinned pixels; 23.6 62.7 nm along two shift axes; 2.8 -68.1 nm on specimen axes Total_Shift = 35.008072 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Measured defocus = 1.29 microns changed by -2.79 to target drift = 0.11 nm/sec Measured defocus = -1.51 microns changed by 0.01 to target drift = 0.10 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7563.tif 27 frames are being saved to U:\krios\20190717\PilA_7564.tif 27 frames are being saved to U:\krios\20190717\PilA_7565.tif 27 frames are being saved to U:\krios\20190717\PilA_7566.tif 27 frames are being saved to U:\krios\20190717\PilA_7567.tif 27 frames are being saved to U:\krios\20190717\PilA_7568.tif Running script 17 at item 403 (76.39, -75.47) Remaining time to fill dewars is 10020 Dewars are NOT busy filling Trimming A by 21 195 170 20, reference by 250 23 20 255 Trimming A by 21 195 170 20, reference by 240 21 185 20 Trimming A by 21 195 170 20, reference by 23 187 20 250 Trimming A by 21 195 170 20, reference by 20 181 180 20 Disparity in stage position after aligning to center of map frame: -0.02 -0.09 Trimming A by 45 30 55 20, reference by 20 181 180 20 Disparity in stage position after aligning to target position: -0.10 -0.26 (2nd move error -0.09 -0.19) Finished aligning to item in 2 rounds Trimming A by 40 36 55 20, reference by 45 30 55 20 -23.3 18.4 unbinned pixels; 98.0 56.1 nm along two shift axes; -66.6 -94.4 nm on specimen axes Total_Shift = 59.338559 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Measured defocus = 1.31 microns changed by -2.91 to target drift = 0.03 nm/sec Measured defocus = -1.66 microns changed by 0.06 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7569.tif 27 frames are being saved to U:\krios\20190717\PilA_7570.tif 27 frames are being saved to U:\krios\20190717\PilA_7571.tif 27 frames are being saved to U:\krios\20190717\PilA_7572.tif 27 frames are being saved to U:\krios\20190717\PilA_7573.tif 27 frames are being saved to U:\krios\20190717\PilA_7574.tif Running script 17 at item 404 (77.74, -78.28) Remaining time to fill dewars is 9960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 105 105 20, reference by 20 181 180 20 Disparity in stage position after aligning to target position: -0.05 -0.21 (2nd move error -0.04 -0.14) Finished aligning to item in 1 rounds Trimming A by 24 105 110 20, reference by 23 105 105 20 -17.8 8.5 unbinned pixels; 54.5 51.2 nm along two shift axes; -29.4 -71.0 nm on specimen axes Total_Shift = 39.426495 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Measured defocus = 1.23 microns changed by -2.93 to target drift = 0.04 nm/sec Measured defocus = -1.68 microns changed by -0.02 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7575.tif 27 frames are being saved to U:\krios\20190717\PilA_7576.tif 27 frames are being saved to U:\krios\20190717\PilA_7577.tif 27 frames are being saved to U:\krios\20190717\PilA_7578.tif 27 frames are being saved to U:\krios\20190717\PilA_7579.tif 27 frames are being saved to U:\krios\20190717\PilA_7580.tif Running script 17 at item 405 (75.12, -79.66) Remaining time to fill dewars is 9720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 95 65 35, reference by 20 181 180 20 Disparity in stage position after aligning to target position: -0.02 -0.31 (2nd move error -0.01 -0.23) Finished aligning to item in 1 rounds Trimming A by 24 100 65 35, reference by 20 95 65 35 -25.7 4.9 unbinned pixels; 51.5 85.3 nm along two shift axes; -13.5 -101.1 nm on specimen axes Total_Shift = 52.350158 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Measured defocus = 1.13 microns changed by -2.93 to target drift = 0.07 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7581.tif 27 frames are being saved to U:\krios\20190717\PilA_7582.tif 27 frames are being saved to U:\krios\20190717\PilA_7583.tif 27 frames are being saved to U:\krios\20190717\PilA_7584.tif 27 frames are being saved to U:\krios\20190717\PilA_7585.tif 27 frames are being saved to U:\krios\20190717\PilA_7586.tif Running script 17 at item 406 (73.80, -76.84) Remaining time to fill dewars is 9480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 35 20 35, reference by 20 181 180 20 Disparity in stage position after aligning to target position: -0.03 -0.36 (2nd move error -0.02 -0.29) Finished aligning to item in 1 rounds Trimming A by 21 35 20 40, reference by 21 35 20 35 -32.5 5.9 unbinned pixels; 64.0 108.0 nm along two shift axes; -16.1 -127.6 nm on specimen axes Total_Shift = 66.005888 Trimming A by 20 40 20 40, reference by 21 35 20 35 -2.3 3.3 unbinned pixels; 15.0 3.2 nm along two shift axes; -12.3 -9.5 nm on specimen axes Total_Shift = 7.960109 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Measured defocus = 1.04 microns changed by -2.94 to target drift = 0.03 nm/sec Measured defocus = -2.00 microns changed by 0.10 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7587.tif 27 frames are being saved to U:\krios\20190717\PilA_7588.tif 27 frames are being saved to U:\krios\20190717\PilA_7589.tif 27 frames are being saved to U:\krios\20190717\PilA_7590.tif 27 frames are being saved to U:\krios\20190717\PilA_7591.tif 27 frames are being saved to U:\krios\20190717\PilA_7592.tif Running script 17 at item 407 (70.82, -71.81) Remaining time to fill dewars is 9240 Dewars are NOT busy filling Trimming A by 260 23 20 255, reference by 250 23 20 255 Trimming A by 260 23 20 255, reference by 240 21 185 20 Trimming A by 260 23 20 255, reference by 23 187 20 250 Trimming A by 260 23 20 255, reference by 20 181 180 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.02 Trimming A by 135 21 20 125, reference by 250 23 20 255 Disparity in stage position after aligning to target position: 0.25 0.16 (2nd move error 0.19 0.16) Finished aligning to item in 2 rounds Trimming A by 135 23 20 125, reference by 135 21 20 125 3.0 10.2 unbinned pixels; 33.6 26.0 nm along two shift axes; -40.3 9.4 nm on specimen axes Total_Shift = 21.221977 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Measured defocus = 0.60 microns changed by -2.60 to target drift = 0.16 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7593.tif 27 frames are being saved to U:\krios\20190717\PilA_7594.tif 27 frames are being saved to U:\krios\20190717\PilA_7595.tif 27 frames are being saved to U:\krios\20190717\PilA_7596.tif 27 frames are being saved to U:\krios\20190717\PilA_7597.tif 27 frames are being saved to U:\krios\20190717\PilA_7598.tif Running script 17 at item 408 (68.21, -73.16) Remaining time to fill dewars is 9120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 135 20 20 165, reference by 250 23 20 255 Disparity in stage position after aligning to target position: 0.13 -0.02 (2nd move error 0.07 -0.01) Finished aligning to item in 1 rounds Trimming A by 130 20 20 165, reference by 135 20 20 165 -2.1 1.4 unbinned pixels; 8.0 5.5 nm along two shift axes; -5.1 -8.5 nm on specimen axes Total_Shift = 5.102586 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Measured defocus = 0.63 microns changed by -2.73 to target drift = 0.09 nm/sec Measured defocus = -2.17 microns changed by 0.07 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7599.tif 27 frames are being saved to U:\krios\20190717\PilA_7600.tif 27 frames are being saved to U:\krios\20190717\PilA_7601.tif 27 frames are being saved to U:\krios\20190717\PilA_7602.tif 27 frames are being saved to U:\krios\20190717\PilA_7603.tif 27 frames are being saved to U:\krios\20190717\PilA_7604.tif Running script 17 at item 409 (69.72, -75.92) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Trimming A by 20 190 20 250, reference by 250 23 20 255 Trimming A by 20 190 20 250, reference by 240 21 185 20 Trimming A by 20 190 20 250, reference by 23 187 20 250 Trimming A by 20 190 20 250, reference by 20 181 180 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.09 Trimming A by 33 25 20 130, reference by 23 187 20 250 Disparity in stage position after aligning to target position: 0.05 0.06 (2nd move error -0.06 0.14) Finished aligning to item in 2 rounds Trimming A by 42 26 20 125, reference by 33 25 20 130 0.9 19.5 unbinned pixels; 70.5 32.6 nm along two shift axes; -76.0 -0.6 nm on specimen axes Total_Shift = 39.029445 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Measured defocus = 0.56 microns changed by -2.76 to target drift = 0.09 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target drift = 0.02 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7605.tif 27 frames are being saved to U:\krios\20190717\PilA_7606.tif 27 frames are being saved to U:\krios\20190717\PilA_7607.tif 27 frames are being saved to U:\krios\20190717\PilA_7608.tif 27 frames are being saved to U:\krios\20190717\PilA_7609.tif 27 frames are being saved to U:\krios\20190717\PilA_7610.tif Running script 17 at item 410 (71.10, -78.73) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 95 20 110, reference by 23 187 20 250 Disparity in stage position after aligning to target position: 0.10 0.09 (2nd move error -0.00 0.15) Finished aligning to item in 1 rounds Trimming A by 20 95 20 105, reference by 20 95 20 110 0.2 2.4 unbinned pixels; 8.7 4.5 nm along two shift axes; -9.6 0.4 nm on specimen axes Total_Shift = 4.918335 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Measured defocus = -0.87 microns changed by -1.43 to target drift = 0.12 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7611.tif 27 frames are being saved to U:\krios\20190717\PilA_7612.tif 27 frames are being saved to U:\krios\20190717\PilA_7613.tif 27 frames are being saved to U:\krios\20190717\PilA_7614.tif 27 frames are being saved to U:\krios\20190717\PilA_7615.tif 27 frames are being saved to U:\krios\20190717\PilA_7616.tif Running script 17 at item 411 (72.40, -81.48) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 150 20 120, reference by 23 187 20 250 Disparity in stage position after aligning to target position: 0.10 0.16 (2nd move error -0.01 0.23) Finished aligning to item in 1 rounds Trimming A by 21 145 65 110, reference by 20 150 20 120 -0.3 27.7 unbinned pixels; 102.3 40.6 nm along two shift axes; -107.7 -7.1 nm on specimen axes Total_Shift = 55.422575 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Measured defocus = 0.48 microns changed by -2.88 to target drift = 0.14 nm/sec Measured defocus = -2.39 microns changed by -0.01 to target drift = 0.05 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7617.tif 27 frames are being saved to U:\krios\20190717\PilA_7618.tif 27 frames are being saved to U:\krios\20190717\PilA_7619.tif 27 frames are being saved to U:\krios\20190717\PilA_7620.tif 27 frames are being saved to U:\krios\20190717\PilA_7621.tif 27 frames are being saved to U:\krios\20190717\PilA_7622.tif Running script 17 at item 412 (116.13, -22.11) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 180 22 195 20, reference by 215 23 20 225 Trimming A by 180 22 195 20, reference by 178 20 190 20 Trimming A by 180 22 195 20, reference by 22 175 20 210 Trimming A by 180 22 195 20, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: -0.05 -0.06 Trimming A by 125 21 80 20, reference by 178 20 190 20 Disparity in stage position after aligning to target position: -0.14 -0.26 (2nd move error -0.09 -0.22) Finished aligning to item in 2 rounds Trimming A by 120 20 75 20, reference by 125 21 80 20 -27.7 26.4 unbinned pixels; 133.2 59.9 nm along two shift axes; -96.9 -113.2 nm on specimen axes Total_Shift = 76.489407 Trimming A by 115 22 75 20, reference by 125 21 80 20 1.4 18.2 unbinned pixels; 65.2 32.6 nm along two shift axes; -71.2 1.7 nm on specimen axes Total_Shift = 36.578733 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Measured defocus = -1.66 microns changed by -0.84 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7623.tif 27 frames are being saved to U:\krios\20190717\PilA_7624.tif 27 frames are being saved to U:\krios\20190717\PilA_7625.tif 27 frames are being saved to U:\krios\20190717\PilA_7626.tif 27 frames are being saved to U:\krios\20190717\PilA_7627.tif 27 frames are being saved to U:\krios\20190717\PilA_7628.tif Running script 17 at item 413 (113.38, -23.85) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 192 21 20 245, reference by 215 23 20 225 Trimming A by 192 21 20 245, reference by 178 20 190 20 Trimming A by 192 21 20 245, reference by 22 175 20 210 Trimming A by 192 21 20 245, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: -0.00 -0.04 Trimming A by 80 20 20 75, reference by 215 23 20 225 Disparity in stage position after aligning to target position: 0.19 0.12 (2nd move error 0.19 0.14) Finished aligning to item in 2 rounds Trimming A by 85 20 20 70, reference by 80 20 20 75 -3.9 -3.2 unbinned pixels; 6.6 18.6 nm along two shift axes; 13.2 -14.3 nm on specimen axes Total_Shift = 9.983866 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -2.60 um Measured defocus = -0.37 microns changed by -2.23 to target drift = 0.14 nm/sec Measured defocus = -2.61 microns changed by 0.01 to target drift = 0.08 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7629.tif 27 frames are being saved to U:\krios\20190717\PilA_7630.tif 27 frames are being saved to U:\krios\20190717\PilA_7631.tif 27 frames are being saved to U:\krios\20190717\PilA_7632.tif 27 frames are being saved to U:\krios\20190717\PilA_7633.tif 27 frames are being saved to U:\krios\20190717\PilA_7634.tif Running script 17 at item 414 (110.78, -25.25) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 66 21 20 145, reference by 215 23 20 225 Disparity in stage position after aligning to target position: 0.20 -0.01 (2nd move error 0.20 0.02) Finished aligning to item in 1 rounds Trimming A by 65 22 20 140, reference by 66 21 20 145 1.7 -5.4 unbinned pixels; 22.0 2.1 nm along two shift axes; 20.6 7.6 nm on specimen axes Total_Shift = 11.268396 Target defocus is: -2.60 um 0.00 degrees Target defocus is: -0.90 um Measured defocus = 1.40 microns changed by -2.30 to target drift = 0.13 nm/sec Measured defocus = -0.86 microns changed by -0.04 to target drift = 0.07 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7635.tif 27 frames are being saved to U:\krios\20190717\PilA_7636.tif 27 frames are being saved to U:\krios\20190717\PilA_7637.tif 27 frames are being saved to U:\krios\20190717\PilA_7638.tif 27 frames are being saved to U:\krios\20190717\PilA_7639.tif 27 frames are being saved to U:\krios\20190717\PilA_7640.tif Running script 17 at item 415 (112.32, -27.97) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Trimming A by 21 171 20 215, reference by 215 23 20 225 Trimming A by 21 171 20 215, reference by 178 20 190 20 Trimming A by 21 171 20 215, reference by 22 175 20 210 Trimming A by 21 171 20 215, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.09 Trimming A by 30 20 20 50, reference by 22 175 20 210 Disparity in stage position after aligning to target position: 0.01 0.08 (2nd move error -0.05 0.14) Finished aligning to item in 2 rounds Trimming A by 35 20 20 50, reference by 30 20 20 50 -1.7 10.7 unbinned pixels; 41.6 9.9 nm along two shift axes; -41.2 -9.0 nm on specimen axes Total_Shift = 21.657741 Target defocus is: -0.90 um 0.00 degrees Target defocus is: -1.00 um Measured defocus = 1.44 microns changed by -2.44 to target drift = 0.17 nm/sec Measured defocus = -0.99 microns changed by -0.01 to target drift = 0.06 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7641.tif 27 frames are being saved to U:\krios\20190717\PilA_7642.tif 27 frames are being saved to U:\krios\20190717\PilA_7643.tif 27 frames are being saved to U:\krios\20190717\PilA_7644.tif 27 frames are being saved to U:\krios\20190717\PilA_7645.tif 27 frames are being saved to U:\krios\20190717\PilA_7646.tif Running script 17 at item 416 (117.67, -31.71) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Trimming A by 23 175 160 20, reference by 215 23 20 225 Trimming A by 23 175 160 20, reference by 178 20 190 20 Trimming A by 23 175 160 20, reference by 22 175 20 210 Trimming A by 23 175 160 20, reference by 23 175 160 20 Disparity in stage position after aligning to center of map frame: 0.00 -0.09 Trimming A by 23 85 70 20, reference by 23 175 160 20 Disparity in stage position after aligning to target position: -0.03 -0.26 (2nd move error -0.03 -0.20) Finished aligning to item in 2 rounds Trimming A by 23 85 75 20, reference by 23 85 70 20 -25.5 7.7 unbinned pixels; 61.6 80.4 nm along two shift axes; -24.4 -101.0 nm on specimen axes Total_Shift = 53.351778 Target defocus is: -1.00 um 0.00 degrees Target defocus is: -1.10 um Measured defocus = 1.53 microns changed by -2.63 to target drift = 0.02 nm/sec Measured defocus = -1.15 microns changed by 0.05 to target drift = 0.03 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7647.tif 27 frames are being saved to U:\krios\20190717\PilA_7648.tif 27 frames are being saved to U:\krios\20190717\PilA_7649.tif 27 frames are being saved to U:\krios\20190717\PilA_7650.tif 27 frames are being saved to U:\krios\20190717\PilA_7651.tif 27 frames are being saved to U:\krios\20190717\PilA_7652.tif Running script 17 at item 417 (114.90, -33.10) Remaining time to fill dewars is 7800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 25 65, reference by 23 175 160 20 Disparity in stage position after aligning to target position: 0.07 -0.34 (2nd move error 0.07 -0.26) Finished aligning to item in 1 rounds Trimming A by 20 85 30 65, reference by 20 80 25 65 -24.1 -2.2 unbinned pixels; 23.6 90.2 nm along two shift axes; 13.5 -93.5 nm on specimen axes Total_Shift = 48.491622 Target defocus is: -1.10 um 0.00 degrees Target defocus is: -1.20 um Measured defocus = 1.43 microns changed by -2.63 to target drift = 0.11 nm/sec Measured defocus = -1.23 microns changed by 0.03 to target drift = 0.11 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7653.tif 27 frames are being saved to U:\krios\20190717\PilA_7654.tif 27 frames are being saved to U:\krios\20190717\PilA_7655.tif 27 frames are being saved to U:\krios\20190717\PilA_7656.tif 27 frames are being saved to U:\krios\20190717\PilA_7657.tif 27 frames are being saved to U:\krios\20190717\PilA_7658.tif Running script 17 at item 418 (110.97, -32.21) Remaining time to fill dewars is 7620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 100 20 115, reference by 22 175 20 210 Disparity in stage position after aligning to target position: 0.10 -0.02 (2nd move error 0.05 0.04) Finished aligning to item in 1 rounds Trimming A by 20 95 20 110, reference by 20 100 20 115 -2.5 -3.0 unbinned pixels; 7.7 13.3 nm along two shift axes; 12.0 -8.9 nm on specimen axes Total_Shift = 7.675443 Target defocus is: -1.20 um 0.00 degrees Target defocus is: -1.30 um Measured defocus = -0.01 microns changed by -1.29 to target drift = 0.00 nm/sec 27 frames are being saved to U:\krios\20190717\PilA_7659.tif 27 frames are being saved to U:\krios\20190717\PilA_7660.tif 27 frames are being saved to U:\krios\20190717\PilA_7661.tif 27 frames are being saved to U:\krios\20190717\PilA_7662.tif 27 frames are being saved to U:\krios\20190717\PilA_7663.tif 27 frames are being saved to U:\krios\20190717\PilA_7664.tif Special option is set NOT to use antialiased reduction instead of binning for all K2/K3 shots Read settings from: C:\Program Files\SerialEM\SerialEMsettings20190212_42K.txt The original displacements in the overlap zones have mean 172.61 maximum 352.26 pixels. After shifting pieces into register, the displacements have mean 107.31 maximum 201.75 pixels. Rough eucentricity: changing Z by 9.20 to 78.87, finished. Z = 0: The original displacements in the overlap zones have mean 54.74 maximum 191.85 pixels. After shifting pieces into register, the displacements have mean 23.22 maximum 70.15 pixels. Map acquired at item # 4 with label 6 saved at Z = 0 Full View shift offset is set to 0.088, -0.323 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Rough eucentricity: changing Z by -1.51 to 77.36, finished.