Dataset acquisition settings:
Pixel size: 1.179A
Dose rate: 4.87e/p/s
Dose per tilt: 3.5/e/A2
Total dose: 143.5e/A2
Tilt range: +/- 60 deg with 3 deg increments
Scheme: Dose symmetric
Exposure time: 1.02s/tilt
Illuminated area: 1.2um
Spot size: 5

Folder structure:
fractions/	- raw camera frames in EER format
TS/		- tilt series in mrc format (individual tilts obtained 
		  by alignment and summation of frames)

Gain reference: 
gainImage_16k.mrc

Notes:
#1
Use the relion_convert_to_tiff tool for rendering the EER movies into TIFF frames. 
We used --eer_grouping 49 and --eer_upsampling 2, resulting in 5 frames (8K x 8K) per tilt. 
#2
The gain reference is provided in 4x super-resolution (16K x 16K) in MRC format. 
In order to generate the multiplicative gain reference required for TIFF processing, 
it needs to be converted. This can also be done with the relion_convert_to_tiff tool 
using the --gain option. Note that the provided 16K gain reference may need to be 
resized to match your intended super-resolution mode (--eer_upsampling) prior to
conversion. In such case use cropping in Fourier space to avoid aliasing. 

For more information refer to:
https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html