#Cryo-electron tomography data acquired on S. pombe cryo-FIB lamellae with defocus only (DEF) or with the additional use of a Volta potential phase plate (VPP); deposition of 20 tomograms (10 per imaging condition) used to train and predict with DeePiCt networks.

Naming for VPP tomograms is TS_001 to TS_010 and for DEF tomograms TS_026 to TS_045 which corresponds to TS_0011 to TS_0020 in the DeePiCt publication.

Raw, unaligned multi-frame micrographs for each tilt of the acquired tilt-series are located in frames folders (.tif file format). Frames were aligned into tilt series stacks in Warp (.st files in metadata folders). Corresponding count reference files (.dm4 files), one for VPP tilt series 001 to 004 plus 010, one for VPP tilt series 005 to 009 (dm4 files), and one for DEF tilt series are provided in the corresponding frames folders.
Tomograms (4-times binned, 13.48 A voxel size) were reconstructed using etomo (IMOD) (with metadata files mdocs_ori in frames folders, .tif files in frames folder). (Sub-) Tomogram reconstruction performed in Warp utilized modiefied acquisition metadata files (.mdocs modified to exclude bad tilts in metadata/mdocs_modified folders) and alignment files from etomo (.mdoc and .xf files in metadata folders).

Comprehensive segmentation volumes (32 bit floats, 928x960, 13.48 A voxel size) of ribosomes, fatty acid synthase (FAS), membranes (for 10 VPP and 5 DEF tomograms), organelles (labels) and cytosol are provided in the labels folders. Voxel labels of individual organelles in labels files are defined in organelle_labels.txt.
Ribosome and FAS particle coordinates (.csv files) are provided in the corresponding particle_lists folders (in .csv format with x, y, z coordinates in columns 1, 2, 3 respectively, 4x binned (original pixel size 3.37A)).