#Cryo-electron tomography data acquired on RPE1 cryo-FIB lamellae; deposition of 3 tomograms (TS_011/0011, TS_012/0012, 0004) used to predict and train DeePiCt networks.

Raw, unaligned multi-frame micrographs for each tilt of the acquired tilt-series are located in frames folder (.tif file format). Frames were aligned into tilt series stacks (.st files in metadata folder). Corresponding count reference files (.dm4 files) are located in the frames folder.
Tomograms (4-times binned, 13.48 A voxel size) were reconstructed using etomo (IMOD) from aligned frame stacks (.st files in metadata folder, .tif files in frames folder), acquisition metadata files (.mdoc) and alignment files (.xf in metadata folder).
Comprehensive ground truth segmentation volumes (32 bit floats, 928x960, 13.48 A voxel size) of actin for all 3 tomograms and of microtubules (MT) for tomogram 0004 are provided in labels folder.
Actin ground truth coordinates (xml files) produced in Amira are located in corresponding actin_ground_truth_coordinates folder. It is important to note that the tomograms may be Y-flipped in Amira with respect to the labels. It is recommended to check before using the coordinates directly.
Filtered tomograms (amplitude spectrum matching filter, DeePiCt) are provided in tomograms/filtered_tomos folder used for actin predictions with DeePiCt (post-processed in labels/predictions folder, raw output in labels/predictions_probability_map folder). TS_011/0011 was cropped along the z-axis to reduce data size.