T. kivui cryo-ET dataset containing HDCR filaments
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Acceleration voltage = 300 kV
Cs = 2.7 mm
Physical pixel size = 3.52 /px
Total exposure per tilt = ~10 e- / A^2 distributed over 10 frames for 8 s
Detector = Gatan K2 with Quantum-LS energy filter (20 slit eV width)

Data structure:

./tomo_correspondence.txt: correspondence information between tomogram numbers and names to interconvert between the TOMOMAN and STOPGAP pipelines and SerialEM/IMOD

./subtomo: subtomogram averaging folders
./subtomo/wedgelist.star: wedgelist file required by STOPGAP (for correspondence between tomogram numbers and names see file tomo_correspondence.txt)
./subtomo/ribo/ribo_tm_bin1_motl_12.star: coordinates and final alignment parameters of ribosome subtomograms at bin1
./subtomo/ribo/subtomograms_bin1/: ribosome subtomograms at bin1 (NovaCTF corrected)
./subtomo/hdcr/hdcr_tm_bin2_motl_12.star: coordinates and final alignment parameters of HDCR subtomograms at bin2
./subtomo/hdcr/subtomograms_bin2/: coordinates and final alignment parameters of HDCR subtomograms at bin2

./tomo: tomography raw data and metadata folders
./tomo/bin4_denoised: tomograms denoised with CRYO-CARE at bin4 (14.08 A/px) (for correspondence between tomogram numbers and names see file tomo_correspondence.txt)
./tomo/raw_stacks/2020-10-22/SerialEM: frame-corrected and averaged tilt stacks and .mdoc files
./tomo/raw_stacks/2020-10-22/frames: raw frames, gain correction and defects list files
./tomo/raw_stacks/2020-10-22/imod: IMOD metadata for reconstruction
./tomo/raw_stacks/2021-02-12/SerialEM: frame-corrected and averaged tilt stacks and .mdoc files
./tomo/raw_stacks/2021-02-12/frames: raw frames, gain correction and defects list files
./tomo/raw_stacks/2021-02-12/imod: IMOD metadata for reconstruction


Contact
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Ricardo Righetto
Biozentrum
University of Basel
ricardo.righetto@unibas.ch