Warning: Camera # 1, K2 Orius, reports a bigger size (2688 x 2672) than the size in the properties file (2072 x 2064) SerialEM Version 3.8.0 beta 64-bit, built May 6 2020 22:31:23 Started 10/22/2020 09:54:22 Last known C2 aperture size was 50 microns. 4 intensity calibrations have been set to work with that size. Use Calibration - Set Aperture Size if the size is now different or you change the aperture. The C2 aperture size was not recorded for 2 beam intensity calibrations. These will work correctly only at the same aperture size as when they were obtained. To fix the calibration file, see the Help for Calibration - Beam Intensity WARNING: Post-exposure actions will be disabled for camera 0 because the camera property StartupDelay is too short. To enable Post-exposure actions, run Camera Timing in the Calibrate menu and update the StartupDelay. Read settings from: C:\Program Files\SerialEM\SerialEMsettings.txt Read properties/calibrations from: C:\ProgramData\SerialEM\ Read scripts from D:\Users\Philipp\Settings\20200409-scripts-scripts.txt Read settings from: D:\Users\Ben\New_settings\20200915.txt Read scripts from D:\Users\Ben\New_settings\20200915-scripts.txt Trimming A by 20 75, reference by 20 75 Angle = -4.40, increment = 0.60, shift = 184.4 20.8 pixels, 0.424 um Y Trimming A by 20 20, reference by 20 75 Angle = -3.20, increment = 1.80, shift = 546.1 62.4 pixels, 1.255 um Y Trimming A by 20 20, reference by 20 75 Angle = -2.13, increment = 2.87, shift = 860.5 99.0 pixels, 1.977 um Y Rough eucentricity: changing Z by -39.51 to -230.69, continuing... Angle = 0.75, increment = 2.87, shift = -12.4 -46.2 pixels, -0.040 um Y Angle = 5.87, increment = 8.00, shift = -18.5 -57.5 pixels, -0.057 um Y Rough eucentricity: changing Z by 0.41 to -230.28, finished. Angle = -4.40, increment = 0.60, shift = 23.9 3.2 pixels, 0.055 um Y Angle = -3.20, increment = 1.80, shift = 61.6 8.2 pixels, 0.142 um Y Angle = 0.41, increment = 5.41, shift = 168.4 20.6 pixels, 0.387 um Y Angle = 3.00, increment = 8.00, shift = 235.3 25.6 pixels, 0.540 um Y Rough eucentricity: changing Z by -3.87 to -234.16, finished. Angle = -4.40, increment = 0.60, shift = -8.1 -0.9 pixels, -0.019 um Y Angle = -3.20, increment = 1.80, shift = -25.9 -3.8 pixels, -0.060 um Y Angle = 0.41, increment = 5.41, shift = -75.0 -11.5 pixels, -0.173 um Y Trimming A by 50 50, reference by 20 20 Angle = 3.00, increment = 8.00, shift = -132.5 -19.5 pixels, -0.306 um Y Rough eucentricity: changing Z by 2.19 to -231.97, finished. Angle = -4.40, increment = 0.60, shift = 15.8 0.8 pixels, 0.036 um Y Angle = -3.20, increment = 1.80, shift = 62.7 5.9 pixels, 0.144 um Y Angle = 0.41, increment = 5.41, shift = 192.4 17.3 pixels, 0.441 um Y Angle = 3.00, increment = 8.00, shift = 274.1 23.2 pixels, 0.628 um Y Rough eucentricity: changing Z by -4.50 to -236.47, finished. Angle = -4.40, increment = 0.60, shift = 16.2 1.1 pixels, 0.037 um Y Angle = -3.20, increment = 1.80, shift = 58.3 6.0 pixels, 0.134 um Y Angle = 0.40, increment = 5.40, shift = 170.8 16.4 pixels, 0.392 um Y Angle = 3.00, increment = 8.00, shift = 248.1 22.7 pixels, 0.569 um Y Rough eucentricity: changing Z by -4.08 to -240.54, finished. Angle = -4.40, increment = 0.60, shift = -62.3 -8.8 pixels, -0.144 um Y Angle = -3.20, increment = 1.80, shift = -173.9 -23.9 pixels, -0.401 um Y Angle = 0.41, increment = 5.41, shift = -534.1 -75.3 pixels, -1.231 um Y Angle = 3.00, increment = 8.00, shift = -798.5 -113.0 pixels, -1.840 um Y Rough eucentricity: changing Z by 13.19 to -227.35, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = -0.000, -0.000 Angle = 18.00, shift = -212.2, -15.5 pixels, 0.019, -0.485 um, IS = -0.019, 0.485 Angle = 11.00, shift = -254.7, -25.7 pixels, 0.007, -0.584 um, IS = -0.026, 1.070 Angle = 4.00, shift = -238.6, -26.5 pixels, 0.001, -0.548 um, IS = -0.026, 1.618 Angle = -4.00, shift = -213.7, -45.2 pixels, -0.048, -0.496 um, IS = 0.021, 2.114 Angle = -11.00, shift = -247.2, -37.3 pixels, -0.022, -0.570 um, IS = 0.043, 2.684 Angle = -18.00, shift = -203.1, -21.0 pixels, 0.004, -0.466 um, IS = 0.039, 3.151 General solution delZ 4.28, delY -0.36, yZero 1.89 solution with assumed YZero 1.87: delZ 4.30, delY -0.74 Refining eucentricity: Fit to 7 positions gives tilt axis Z displacement of 4.28 microns and lateral displacement of 0.36 microns Measured defocus = -3.48 microns changed by -1.02 to target drift = 0.27 nm/sec 40 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\000_-10.0_Oct22_10.57.24.tif Measured defocus = -4.95 microns changed by 0.45 to target drift = 0.19 nm/sec Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.000 um; position 0.000, -0.000 Drift reached 0.3 A/sec after 5.1 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.02 nm/sec Tracking image shifted 0.000, 0.000 um; position 0.000, -0.000 Running script # 14 0.0 0.1 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes Rate = 0.18433 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_000_-10.0_Oct22_11.16.00.tif Record image shifted 0.002, -0.004 um Tracking reference shifted by -11.7,-5.6 pixels, 0.002,-0.004 um Saved Z = 0 at 0.00 min Tilt = -10.00 Mean = 112 Exp = 1.560 X/Y/Z = -0.002 0.004 3.945 Need regular tracking - Need autofocus Tracking image shifted -0.003, -0.212 um; position 0.001, 0.216 Drift reached 0.9 A/sec after 5.0 sec, going on Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.04 nm/sec Tracking image shifted -0.003, -0.001 um; position 0.005, 0.217 Running script # 14 -0.3 0.4 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.1 -0.1 nm on specimen axes Rate = 0.93055 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_001_-12.0_Oct22_11.16.39.tif Record image shifted 0.001, 0.007 um Tracking reference shifted by 18.7,-3.2 pixels, 0.001,0.007 um Saved Z = 1 at 0.66 min Tilt = -12.00 Mean = 110 Exp = 1.532 X/Y/Z = 0.004 0.210 3.985 Predicted X = 0.010 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.417 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 4.026 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.197 um; position 0.005, 0.221 Drift reached 1.6 A/sec after 5.0 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.03 nm/sec Tracking image shifted 0.003, 0.001 um; position 0.002, 0.220 Running script # 14 -0.2 0.3 unbinned pixels; 0.1 0.0 nm along two shift axes; -0.1 -0.1 nm on specimen axes Rate = 0.66787 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_002_-14.0_Oct22_11.17.18.tif Record image shifted 0.000, 0.007 um Tracking reference shifted by 19.5,-2.3 pixels, 0.000,0.007 um Saved Z = 2 at 1.31 min Tilt = -14.00 Mean = 110 Exp = 1.532 X/Y/Z = 0.002 0.213 4.060 Errors in X/Y Prediction -0.008 -0.204 um, 0.6 15.6 % Error in Z Prediction 0.035 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.074 um; position -0.007, -0.071 Drift reached 3.7 A/sec after 5.1 sec, going on Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.14 nm/sec Tracking image shifted 0.002, 0.006 um; position -0.008, -0.078 Running script # 14 1.6 0.2 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.1 0.6 nm on specimen axes Rate = 2.90277 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_003_-8.0_Oct22_11.18.01.tif Record image shifted 0.000, -0.004 um Tracking reference shifted by -12.3,-0.3 pixels, 0.000,-0.004 um Saved Z = 3 at 2.02 min Tilt = -8.00 Mean = 110 Exp = 1.532 X/Y/Z = -0.009 -0.074 3.878 Need regular tracking - Need autofocus Tracking image shifted 0.004, 0.100 um; position -0.012, -0.175 Drift reached 1.2 A/sec after 5.2 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.09 nm/sec Tracking image shifted 0.002, -0.003 um; position -0.013, -0.171 Running script # 14 -0.8 0.5 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.2 -0.3 nm on specimen axes Rate = 1.67501 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_004_-6.0_Oct22_11.18.44.tif Record image shifted -0.000, -0.009 um Tracking reference shifted by -25.8,2.9 pixels, -0.000,-0.009 um Saved Z = 4 at 2.73 min Tilt = -6.00 Mean = 110 Exp = 1.528 X/Y/Z = -0.013 -0.162 3.832 Predicted X = 0.006 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = 0.352 StdErr = 0.152 nFit = 3 nDrop = 0 Predicted Z = 4.112 StdErr = 0.026 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, 0.215 um; position 0.009, 0.138 Drift reached 2.0 A/sec after 5.1 sec, going on Running script # 14 -1.7 1.3 unbinned pixels; 0.6 0.4 nm along two shift axes; -0.4 -0.6 nm on specimen axes Rate = 3.784 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_005_-16.0_Oct22_11.19.14.tif Record image shifted 0.004, 0.004 um Tracking reference shifted by 10.7,-12.5 pixels, 0.004,0.004 um Saved Z = 5 at 3.23 min Tilt = -16.00 Mean = 110 Exp = 1.538 X/Y/Z = 0.006 0.135 4.112 Errors in X/Y Prediction -0.000 -0.217 um, 0.0 16.6 % Predicted X = 0.006 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.111 StdErr = 0.061 nFit = 3 nDrop = 0 Predicted Z = 4.170 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.021 um; position -0.000, 0.131 Drift reached 2.0 A/sec after 5.2 sec, going on Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.07 nm/sec Tracking image shifted -0.000, 0.002 um; position 0.000, 0.128 Running script # 14 0.8 0.3 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.4686 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_006_-18.0_Oct22_11.19.54.tif Record image shifted -0.004, 0.010 um Tracking reference shifted by 28.9,9.1 pixels, -0.004,0.010 um Saved Z = 6 at 3.90 min Tilt = -18.00 Mean = 109 Exp = 1.544 X/Y/Z = 0.004 0.118 4.224 Errors in X/Y Prediction -0.002 0.007 um, 0.1 0.5 % Error in Z Prediction 0.054 Predicted X = -0.017 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.250 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.786 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.010, -0.158 um; position -0.007, -0.092 Drift reached 2.6 A/sec after 5.1 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.11 nm/sec Tracking image shifted -0.002, 0.005 um; position -0.005, -0.098 Running script # 14 1.4 0.5 unbinned pixels; 0.0 0.5 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.56991 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_007_-4.0_Oct22_11.20.38.tif Record image shifted 0.000, -0.005 um Tracking reference shifted by -13.4,1.0 pixels, 0.000,-0.005 um Saved Z = 7 at 4.64 min Tilt = -4.00 Mean = 110 Exp = 1.526 X/Y/Z = -0.004 -0.094 3.811 Errors in X/Y Prediction 0.013 0.156 um, 1.0 11.9 % Error in Z Prediction 0.025 Predicted X = -0.004 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.129 StdErr = 0.116 nFit = 3 nDrop = 0 Predicted Z = 3.774 StdErr = 0.019 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.019 um; position -0.010, -0.111 Drift reached 2.1 A/sec after 5.3 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.11 nm/sec Tracking image shifted -0.001, -0.005 um; position -0.009, -0.105 Running script # 14 0.5 1.4 unbinned pixels; 0.4 0.3 nm along two shift axes; -0.5 0.1 nm on specimen axes Rate = 2.60072 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_008_-2.0_Oct22_11.21.20.tif Record image shifted 0.000, -0.010 um Tracking reference shifted by -27.6,1.8 pixels, 0.000,-0.010 um Saved Z = 8 at 5.34 min Tilt = -2.00 Mean = 110 Exp = 1.529 X/Y/Z = -0.008 -0.095 3.729 Errors in X/Y Prediction -0.004 0.034 um, 0.3 2.6 % Error in Z Prediction -0.045 Predicted X = 0.006 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.060 StdErr = 0.046 nFit = 3 nDrop = 0 Predicted Z = 4.302 StdErr = 0.006 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, 0.056 um; position 0.012, 0.003 Drift reached 0.5 A/sec after 5.2 sec, going on Running script # 14 -0.0 0.7 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.3 -0.0 nm on specimen axes Rate = 1.30275 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_009_-20.0_Oct22_11.21.56.tif Record image shifted 0.000, 0.003 um Tracking reference shifted by 8.7,-0.9 pixels, 0.000,0.003 um Saved Z = 9 at 5.93 min Tilt = -20.00 Mean = 109 Exp = 1.552 X/Y/Z = 0.013 0.000 4.302 Errors in X/Y Prediction 0.006 -0.060 um, 0.5 4.6 % Predicted X = 0.019 StdErr = 0.010 nFit = 4 nDrop = 0 Predicted Y = -0.097 StdErr = 0.107 nFit = 4 nDrop = 0 Predicted Z = 4.382 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.012, -0.059 um; position 0.007, -0.038 Drift reached 1.9 A/sec after 5.1 sec, going on Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.08 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.008, -0.041 Running script # 14 0.8 0.3 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.55277 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_010_-22.0_Oct22_11.22.35.tif Record image shifted -0.000, 0.011 um Tracking reference shifted by 30.6,-1.7 pixels, -0.000,0.011 um Saved Z = 10 at 6.59 min Tilt = -22.00 Mean = 109 Exp = 1.571 X/Y/Z = 0.009 -0.053 4.299 Errors in X/Y Prediction -0.010 0.044 um, 0.8 3.4 % Error in Z Prediction -0.084 Predicted X = -0.004 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.050 StdErr = 0.052 nFit = 3 nDrop = 0 Predicted Z = 3.689 StdErr = 0.046 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.007, -0.037 um; position 0.003, -0.013 Drift reached 2.9 A/sec after 5.1 sec, going on Running script # 14 2.4 0.8 unbinned pixels; 0.0 0.9 nm along two shift axes; -0.4 0.8 nm on specimen axes Rate = 4.39912 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_011_0.0_Oct22_11.23.07.tif Record image shifted -0.001, -0.001 um Tracking reference shifted by -3.3,2.9 pixels, -0.001,-0.001 um Saved Z = 11 at 7.12 min Tilt = 0.00 Mean = 110 Exp = 1.534 X/Y/Z = 0.005 -0.015 3.689 Errors in X/Y Prediction 0.009 0.035 um, 0.7 2.7 % Predicted X = 0.007 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = 0.011 StdErr = 0.061 nFit = 3 nDrop = 0 Predicted Z = 3.637 StdErr = 0.061 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, 0.022 um; position -0.002, -0.011 Drift reached 2.9 A/sec after 5.1 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.19 nm/sec Tracking image shifted -0.000, -0.006 um; position -0.001, -0.003 Running script # 14 -2.9 0.9 unbinned pixels; 0.6 0.9 nm along two shift axes; -0.2 -1.0 nm on specimen axes Rate = 5.34126 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_012_2.0_Oct22_11.23.49.tif Record image shifted 0.000, -0.014 um Tracking reference shifted by -39.4,2.5 pixels, 0.000,-0.014 um Saved Z = 12 at 7.83 min Tilt = 2.00 Mean = 109 Exp = 1.538 X/Y/Z = -0.001 0.012 3.601 Errors in X/Y Prediction -0.008 0.001 um, 0.6 0.0 % Error in Z Prediction -0.036 Predicted X = 0.013 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.142 StdErr = 0.065 nFit = 5 nDrop = 0 Predicted Z = 4.373 StdErr = 0.057 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, 0.029 um; position 0.014, -0.170 Drift reached 0.8 A/sec after 5.1 sec, going on Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.03 nm/sec Tracking image shifted -0.000, -0.000 um; position 0.015, -0.170 Running script # 14 -0.0 0.5 unbinned pixels; 0.2 0.0 nm along two shift axes; -0.2 -0.0 nm on specimen axes Rate = 0.79711 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_013_-24.0_Oct22_11.24.31.tif Record image shifted -0.000, 0.005 um Tracking reference shifted by 15.4,-1.1 pixels, -0.000,0.005 um Saved Z = 13 at 8.53 min Tilt = -24.00 Mean = 109 Exp = 1.580 X/Y/Z = 0.015 -0.175 4.346 Errors in X/Y Prediction 0.002 -0.034 um, 0.1 2.6 % Error in Z Prediction -0.027 Predicted X = 0.015 StdErr = 0.014 nFit = 4 nDrop = 0 Predicted Y = -0.283 StdErr = 0.049 nFit = 6 nDrop = 0 Predicted Z = 4.383 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, -0.054 um; position 0.011, -0.230 Drift reached 1.7 A/sec after 5.1 sec, going on Running script # 14 1.6 0.7 unbinned pixels; 0.0 0.6 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 2.98762 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_014_-26.0_Oct22_11.24.59.tif Record image shifted -0.000, 0.009 um Tracking reference shifted by 24.3,-1.1 pixels, -0.000,0.009 um Saved Z = 14 at 8.99 min Tilt = -26.00 Mean = 109 Exp = 1.598 X/Y/Z = 0.012 -0.240 4.383 Errors in X/Y Prediction -0.003 0.044 um, 0.2 3.4 % Predicted X = 0.001 StdErr = 0.024 nFit = 4 nDrop = 0 Predicted Y = 0.086 StdErr = 0.090 nFit = 4 nDrop = 0 Predicted Z = 3.530 StdErr = 0.016 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.014, -0.010 um; position 0.014, 0.096 Drift reached 1.2 A/sec after 5.1 sec, going on Running script # 14 0.8 0.5 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.69784 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_015_4.0_Oct22_11.25.33.tif Record image shifted -0.001, -0.002 um Tracking reference shifted by -5.2,2.6 pixels, -0.001,-0.002 um Saved Z = 15 at 9.55 min Tilt = 4.00 Mean = 110 Exp = 1.547 X/Y/Z = 0.016 0.097 3.530 Errors in X/Y Prediction 0.015 0.011 um, 1.1 0.8 % Predicted X = 0.024 StdErr = 0.028 nFit = 4 nDrop = 0 Predicted Y = 0.188 StdErr = 0.051 nFit = 5 nDrop = 0 Predicted Z = 3.460 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.015, 0.102 um; position 0.009, 0.086 Drift reached 3.2 A/sec after 5.1 sec, going on Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.19 nm/sec Tracking image shifted 0.001, -0.007 um; position 0.008, 0.094 Running script # 14 -2.8 1.0 unbinned pixels; 0.6 0.8 nm along two shift axes; -0.2 -1.0 nm on specimen axes Rate = 5.16563 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_016_6.0_Oct22_11.26.15.tif Record image shifted 0.001, -0.013 um Tracking reference shifted by -36.4,1.6 pixels, 0.001,-0.013 um Saved Z = 16 at 10.25 min Tilt = 6.00 Mean = 109 Exp = 1.550 X/Y/Z = 0.008 0.108 3.434 Errors in X/Y Prediction -0.016 -0.080 um, 1.2 6.1 % Error in Z Prediction -0.026 Predicted X = 0.014 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Y = -0.359 StdErr = 0.055 nFit = 6 nDrop = 0 Predicted Z = 4.424 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.009, -0.023 um; position 0.023, -0.336 Drift reached 0.3 A/sec after 5.1 sec, going on Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Tracking image shifted -0.002, 0.000 um; position 0.025, -0.336 Running script # 14 -0.0 0.2 unbinned pixels; 0.1 0.0 nm along two shift axes; -0.1 -0.0 nm on specimen axes Rate = 0.42974 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_017_-28.0_Oct22_11.26.58.tif Record image shifted 0.000, 0.006 um Tracking reference shifted by 17.6,-2.4 pixels, 0.000,0.006 um Saved Z = 17 at 10.98 min Tilt = -28.00 Mean = 109 Exp = 1.618 X/Y/Z = 0.025 -0.342 4.431 Errors in X/Y Prediction 0.011 0.017 um, 0.8 1.3 % Error in Z Prediction 0.008 Predicted X = 0.027 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.425 StdErr = 0.039 nFit = 6 nDrop = 0 Predicted Z = 4.471 StdErr = 0.007 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.016 um; position 0.022, -0.409 Drift reached 0.5 A/sec after 5.1 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Tracking image shifted -0.001, 0.002 um; position 0.023, -0.411 Running script # 14 0.3 0.3 unbinned pixels; 0.1 0.1 nm along two shift axes; -0.1 0.1 nm on specimen axes Rate = 0.69656 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_018_-30.0_Oct22_11.27.38.tif Record image shifted 0.000, 0.007 um Tracking reference shifted by 19.3,-2.4 pixels, 0.000,0.007 um Saved Z = 18 at 11.64 min Tilt = -30.00 Mean = 108 Exp = 1.635 X/Y/Z = 0.023 -0.418 4.486 Errors in X/Y Prediction -0.004 0.007 um, 0.3 0.5 % Error in Z Prediction 0.015 Predicted X = 0.012 StdErr = 0.031 nFit = 4 nDrop = 0 Predicted Y = 0.176 StdErr = 0.054 nFit = 6 nDrop = 0 Predicted Z = 3.367 StdErr = 0.025 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.011, 0.025 um; position 0.023, 0.152 Drift reached 1.5 A/sec after 5.1 sec, going on Running script # 14 -2.9 1.9 unbinned pixels; 1.0 0.7 nm along two shift axes; -0.6 -1.1 nm on specimen axes Rate = 6.08421 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_019_8.0_Oct22_11.28.12.tif Record image shifted -0.000, -0.009 um Tracking reference shifted by -26.2,3.3 pixels, -0.000,-0.009 um Saved Z = 19 at 12.20 min Tilt = 8.00 Mean = 109 Exp = 1.558 X/Y/Z = 0.024 0.163 3.367 Errors in X/Y Prediction 0.013 -0.014 um, 1.0 1.0 % Predicted X = 0.025 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.187 StdErr = 0.038 nFit = 6 nDrop = 0 Predicted Z = 3.293 StdErr = 0.029 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, 0.084 um; position 0.018, 0.103 Drift reached 1.9 A/sec after 5.1 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.08 nm/sec Tracking image shifted 0.000, -0.001 um; position 0.018, 0.104 Running script # 14 -0.3 0.9 unbinned pixels; 0.3 0.0 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.69649 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_020_10.0_Oct22_11.28.54.tif Record image shifted 0.001, -0.004 um Tracking reference shifted by -12.1,-0.7 pixels, 0.001,-0.004 um Saved Z = 20 at 12.90 min Tilt = 10.00 Mean = 109 Exp = 1.572 X/Y/Z = 0.018 0.108 3.239 Errors in X/Y Prediction -0.007 -0.079 um, 0.5 6.1 % Error in Z Prediction -0.054 Predicted X = 0.029 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Y = -0.513 StdErr = 0.037 nFit = 6 nDrop = 0 Predicted Z = 4.528 StdErr = 0.007 nFit = 4 nDrop = 1 Need regular tracking - Tracking image shifted -0.008, 0.042 um; position 0.037, -0.555 Drift reached 1.5 A/sec after 5.3 sec, going on Running script # 14 1.3 1.2 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.5 0.4 nm on specimen axes Rate = 3.06072 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_021_-32.0_Oct22_11.29.26.tif Record image shifted -0.001, 0.007 um Tracking reference shifted by 19.5,-0.3 pixels, -0.001,0.007 um Saved Z = 21 at 13.44 min Tilt = -32.00 Mean = 109 Exp = 1.659 X/Y/Z = 0.039 -0.563 4.528 Errors in X/Y Prediction 0.010 -0.050 um, 0.7 3.8 % Predicted X = 0.043 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.701 StdErr = 0.036 nFit = 5 nDrop = 0 Predicted Z = 4.574 StdErr = 0.007 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.080 um; position 0.038, -0.621 Drift reached 2.1 A/sec after 5.3 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.002 um; position 0.039, -0.624 Running script # 14 0.5 0.2 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 0.92782 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_022_-34.0_Oct22_11.30.07.tif Record image shifted 0.000, 0.009 um Tracking reference shifted by 25.9,-3.5 pixels, 0.000,0.009 um Saved Z = 22 at 14.13 min Tilt = -34.00 Mean = 108 Exp = 1.682 X/Y/Z = 0.039 -0.633 4.530 Errors in X/Y Prediction -0.004 0.068 um, 0.3 5.2 % Error in Z Prediction -0.044 Predicted X = 0.027 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = 0.098 StdErr = 0.058 nFit = 6 nDrop = 0 Predicted Z = 3.173 StdErr = 0.034 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.005, -0.073 um; position 0.032, 0.171 Drift reached 5.1 A/sec after 5.1 sec, going on Running script # 14 -5.3 2.0 unbinned pixels; 1.3 1.5 nm along two shift axes; -0.5 -1.9 nm on specimen axes Rate = 9.83845 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_023_12.0_Oct22_11.30.42.tif Record image shifted -0.002, -0.017 um Tracking reference shifted by -48.9,9.2 pixels, -0.002,-0.017 um Saved Z = 23 at 14.70 min Tilt = 12.00 Mean = 109 Exp = 1.584 X/Y/Z = 0.035 0.193 3.173 Errors in X/Y Prediction 0.009 0.095 um, 0.7 7.2 % Predicted X = 0.037 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.211 StdErr = 0.095 nFit = 5 nDrop = 0 Predicted Z = 3.091 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, 0.076 um; position 0.033, 0.135 Drift reached 4.0 A/sec after 5.1 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.10 nm/sec Tracking image shifted -0.002, 0.003 um; position 0.037, 0.130 Running script # 14 -1.0 1.8 unbinned pixels; 0.7 0.1 nm along two shift axes; -0.6 -0.4 nm on specimen axes Rate = 3.5641 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_024_14.0_Oct22_11.31.23.tif Record image shifted 0.001, 0.001 um Tracking reference shifted by 3.0,-4.0 pixels, 0.001,0.001 um Saved Z = 24 at 15.40 min Tilt = 14.00 Mean = 109 Exp = 1.601 X/Y/Z = 0.036 0.129 3.037 Errors in X/Y Prediction -0.001 -0.081 um, 0.1 6.2 % Error in Z Prediction -0.054 Predicted X = 0.049 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.762 StdErr = 0.042 nFit = 6 nDrop = 0 Predicted Z = 4.579 StdErr = 0.025 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, 0.020 um; position 0.049, -0.782 Drift reached 0.9 A/sec after 5.4 sec, going on Running script # 14 1.1 1.3 unbinned pixels; 0.3 0.5 nm along two shift axes; -0.5 0.4 nm on specimen axes Rate = 2.98032 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_025_-36.0_Oct22_11.31.57.tif Record image shifted -0.001, 0.008 um Tracking reference shifted by 23.1,1.7 pixels, -0.001,0.008 um Saved Z = 25 at 15.96 min Tilt = -36.00 Mean = 108 Exp = 1.711 X/Y/Z = 0.051 -0.791 4.579 Errors in X/Y Prediction 0.002 -0.029 um, 0.1 2.2 % Predicted X = 0.055 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.928 StdErr = 0.062 nFit = 5 nDrop = 0 Predicted Z = 4.617 StdErr = 0.028 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.104 um; position 0.050, -0.824 Drift reached 1.4 A/sec after 5.4 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.052, -0.828 Running script # 14 0.5 0.4 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.15612 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_026_-38.0_Oct22_11.32.38.tif Record image shifted 0.001, 0.009 um Tracking reference shifted by 23.7,-6.0 pixels, 0.001,0.009 um Saved Z = 26 at 16.64 min Tilt = -38.00 Mean = 108 Exp = 1.744 X/Y/Z = 0.051 -0.836 4.563 Errors in X/Y Prediction -0.004 0.092 um, 0.3 7.0 % Error in Z Prediction -0.054 Predicted X = 0.048 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = 0.114 StdErr = 0.109 nFit = 5 nDrop = 0 Predicted Z = 2.939 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, -0.049 um; position 0.046, 0.162 Drift reached 3.6 A/sec after 5.2 sec, going on Running script # 14 -6.5 2.0 unbinned pixels; 1.4 1.9 nm along two shift axes; -0.5 -2.3 nm on specimen axes Rate = 11.84697 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_027_16.0_Oct22_11.33.13.tif Record image shifted -0.002, -0.017 um Tracking reference shifted by -46.3,9.8 pixels, -0.002,-0.017 um Saved Z = 27 at 17.23 min Tilt = 16.00 Mean = 109 Exp = 1.616 X/Y/Z = 0.050 0.183 2.939 Errors in X/Y Prediction 0.002 0.069 um, 0.1 5.3 % Predicted X = 0.054 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.208 StdErr = 0.111 nFit = 5 nDrop = 0 Predicted Z = 2.840 StdErr = 0.005 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 25 25, reference by 20 20 Tracking image shifted 0.004, 0.117 um; position 0.051, 0.091 Drift reached 3.4 A/sec after 5.2 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.19 nm/sec Tracking image shifted -0.003, -0.002 um; position 0.056, 0.093 Running script # 14 -1.0 2.3 unbinned pixels; 0.9 0.1 nm along two shift axes; -0.8 -0.4 nm on specimen axes Rate = 4.42296 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_028_18.0_Oct22_11.33.56.tif Record image shifted 0.002, 0.001 um Tracking reference shifted by 1.6,-4.9 pixels, 0.002,0.001 um Saved Z = 28 at 17.94 min Tilt = 18.00 Mean = 109 Exp = 1.626 X/Y/Z = 0.055 0.093 2.863 Errors in X/Y Prediction 0.001 -0.115 um, 0.0 8.8 % Error in Z Prediction 0.024 Predicted X = 0.059 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.912 StdErr = 0.075 nFit = 5 nDrop = 0 Predicted Z = 4.596 StdErr = 0.025 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, 0.025 um; position 0.063, -0.937 Drift reached 1.8 A/sec after 5.3 sec, going on Running script # 14 1.7 1.6 unbinned pixels; 0.3 0.8 nm along two shift axes; -0.6 0.6 nm on specimen axes Rate = 4.22187 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_029_-40.0_Oct22_11.34.30.tif Record image shifted -0.001, 0.008 um Tracking reference shifted by 23.1,1.7 pixels, -0.001,0.008 um Saved Z = 29 at 18.51 min Tilt = -40.00 Mean = 107 Exp = 1.770 X/Y/Z = 0.066 -0.947 4.596 Errors in X/Y Prediction 0.007 -0.035 um, 0.5 2.7 % Predicted X = 0.071 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -1.029 StdErr = 0.076 nFit = 5 nDrop = 0 Predicted Z = 4.621 StdErr = 0.028 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.051 um; position 0.064, -0.978 Drift reached 1.7 A/sec after 5.6 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.066, -0.982 Running script # 14 0.8 0.6 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.69373 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_030_-42.0_Oct22_11.35.17.tif Record image shifted 0.001, 0.010 um Tracking reference shifted by 28.8,-6.2 pixels, 0.001,0.010 um Saved Z = 30 at 19.29 min Tilt = -42.00 Mean = 107 Exp = 1.814 X/Y/Z = 0.065 -0.992 4.595 Errors in X/Y Prediction -0.006 0.037 um, 0.4 2.8 % Error in Z Prediction -0.026 Predicted X = 0.074 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -1.022 StdErr = 0.066 nFit = 5 nDrop = 0 Predicted Z = 4.611 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.013, 0.044 um; position 0.061, -1.066 Drift reached 1.7 A/sec after 5.5 sec, going on Running script # 14 0.5 1.7 unbinned pixels; 0.5 0.4 nm along two shift axes; -0.6 0.1 nm on specimen axes Rate = 3.09403 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_031_-44.0_Oct22_11.35.46.tif Record image shifted 0.000, 0.008 um Tracking reference shifted by 21.4,-3.1 pixels, 0.000,0.008 um Saved Z = 31 at 19.78 min Tilt = -44.00 Mean = 107 Exp = 1.857 X/Y/Z = 0.062 -1.074 4.611 Errors in X/Y Prediction -0.012 -0.052 um, 0.9 4.0 % Predicted X = 0.066 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = 0.013 StdErr = 0.105 nFit = 5 nDrop = 0 Predicted Z = 2.765 StdErr = 0.018 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.008, -0.139 um; position 0.058, 0.152 Drift reached 2.1 A/sec after 5.3 sec, going on Running script # 14 -4.1 3.0 unbinned pixels; 1.5 1.0 nm along two shift axes; -0.9 -1.5 nm on specimen axes Rate = 8.84987 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_032_20.0_Oct22_11.36.22.tif Record image shifted -0.001, -0.009 um Tracking reference shifted by -24.9,6.3 pixels, -0.001,-0.009 um Saved Z = 32 at 20.37 min Tilt = 20.00 Mean = 108 Exp = 1.648 X/Y/Z = 0.061 0.163 2.765 Errors in X/Y Prediction -0.005 0.150 um, 0.4 11.5 % Predicted X = 0.067 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.185 StdErr = 0.115 nFit = 5 nDrop = 0 Predicted Z = 2.671 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 30 30, reference by 20 20 Tracking image shifted 0.004, 0.107 um; position 0.063, 0.079 Drift reached 3.7 A/sec after 5.3 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.17 nm/sec Tracking image shifted -0.004, -0.004 um; position 0.069, 0.083 Running script # 14 -0.5 2.2 unbinned pixels; 0.8 0.1 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.02504 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_033_22.0_Oct22_11.37.05.tif Record image shifted 0.001, -0.007 um Tracking reference shifted by -20.1,-2.2 pixels, 0.001,-0.007 um Saved Z = 33 at 21.09 min Tilt = 22.00 Mean = 108 Exp = 1.678 X/Y/Z = 0.068 0.091 2.656 Errors in X/Y Prediction 0.001 -0.095 um, 0.1 7.2 % Error in Z Prediction -0.015 Predicted X = 0.074 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.057 StdErr = 0.121 nFit = 5 nDrop = 0 Predicted Z = 2.567 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.008, -0.076 um; position 0.066, 0.133 Drift reached 2.9 A/sec after 5.3 sec, going on Running script # 14 -7.0 2.4 unbinned pixels; 1.6 2.0 nm along two shift axes; -0.6 -2.5 nm on specimen axes Rate = 12.94301 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_034_24.0_Oct22_11.37.37.tif Record image shifted -0.002, -0.016 um Tracking reference shifted by -45.3,9.8 pixels, -0.002,-0.016 um Saved Z = 34 at 21.70 min Tilt = 24.00 Mean = 108 Exp = 1.702 X/Y/Z = 0.070 0.153 2.567 Errors in X/Y Prediction -0.005 0.096 um, 0.4 7.4 % Predicted X = 0.061 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -1.148 StdErr = 0.049 nFit = 5 nDrop = 0 Predicted Z = 4.627 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.017, -0.022 um; position 0.077, -1.126 Drift reached 2.4 A/sec after 5.5 sec, going on Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.13 nm/sec Tracking image shifted -0.002, 0.005 um; position 0.080, -1.133 Running script # 14 1.5 0.8 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 2.9137 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_035_-46.0_Oct22_11.38.29.tif Record image shifted -0.000, 0.012 um Tracking reference shifted by 33.4,-2.0 pixels, -0.000,0.012 um Saved Z = 35 at 22.58 min Tilt = -46.00 Mean = 107 Exp = 1.907 X/Y/Z = 0.080 -1.145 4.631 Errors in X/Y Prediction 0.020 0.003 um, 1.5 0.2 % Error in Z Prediction 0.003 Predicted X = 0.085 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = -1.201 StdErr = 0.041 nFit = 5 nDrop = 0 Predicted Z = 4.647 StdErr = 0.002 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.008, 0.024 um; position 0.076, -1.225 Drift reached 0.7 A/sec after 5.8 sec, going on Running script # 14 0.3 1.4 unbinned pixels; 0.4 0.3 nm along two shift axes; -0.5 0.0 nm on specimen axes Rate = 2.52225 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_036_-48.0_Oct22_11.39.05.tif Record image shifted -0.001, 0.008 um Tracking reference shifted by 23.7,0.5 pixels, -0.001,0.008 um Saved Z = 36 at 23.09 min Tilt = -48.00 Mean = 107 Exp = 1.963 X/Y/Z = 0.078 -1.234 4.647 Errors in X/Y Prediction -0.006 -0.033 um, 0.5 2.5 % Predicted X = 0.089 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = -1.335 StdErr = 0.018 nFit = 5 nDrop = 0 Predicted Z = 4.664 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.011, -0.063 um; position 0.078, -1.272 Drift reached 1.7 A/sec after 5.7 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.002 um; position 0.080, -1.275 Running script # 14 0.6 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.788 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_037_-50.0_Oct22_11.39.47.tif Record image shifted 0.001, 0.011 um Tracking reference shifted by 31.9,-6.0 pixels, 0.001,0.011 um Saved Z = 37 at 23.80 min Tilt = -50.00 Mean = 106 Exp = 2.025 X/Y/Z = 0.079 -1.287 4.621 Errors in X/Y Prediction -0.010 0.048 um, 0.8 3.7 % Error in Z Prediction -0.043 Predicted X = 0.078 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -1.352 StdErr = 0.021 nFit = 5 nDrop = 0 Predicted Z = 4.635 StdErr = 0.029 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, 0.006 um; position 0.075, -1.358 Drift reached 0.6 A/sec after 5.7 sec, going on Running script # 14 Trimming A by 30 20 20 20, reference by 30 20 20 20 Trimming A by 30 20 20 20, reference by 30 20 20 20 0.6 1.1 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.2 nm on specimen axes Rate = 2.27325 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_038_-52.0_Oct22_11.40.17.tif Record image shifted -0.001, 0.010 um Tracking reference shifted by 29.2,-0.4 pixels, -0.001,0.010 um Saved Z = 38 at 24.30 min Tilt = -52.00 Mean = 106 Exp = 2.097 X/Y/Z = 0.076 -1.368 4.635 Errors in X/Y Prediction -0.001 -0.017 um, 0.1 1.3 % Predicted X = 0.075 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.198 StdErr = 0.117 nFit = 5 nDrop = 0 Predicted Z = 2.471 StdErr = 0.025 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 25, reference by 20 20 Tracking image shifted -0.016, 0.026 um; position 0.091, 0.172 Drift reached 4.8 A/sec after 5.3 sec, going on Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.30 nm/sec Tracking image shifted -0.000, -0.013 um; position 0.091, 0.188 Running script # 14 Trimming A by 20 20 20 25, reference by 20 20 20 25 Trimming A by 20 20 20 25, reference by 20 20 20 25 -4.4 1.7 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.5 -1.6 nm on specimen axes Rate = 8.23354 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_039_26.0_Oct22_11.41.06.tif Record image shifted 0.003, -0.003 um Tracking reference shifted by -10.4,-6.3 pixels, 0.003,-0.003 um Saved Z = 39 at 25.11 min Tilt = 26.00 Mean = 108 Exp = 1.726 X/Y/Z = 0.089 0.193 2.406 Errors in X/Y Prediction 0.015 -0.006 um, 1.1 0.4 % Error in Z Prediction -0.066 Predicted X = 0.097 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.232 StdErr = 0.108 nFit = 5 nDrop = 0 Predicted Z = 2.299 StdErr = 0.028 nFit = 3 nDrop = 0 Need regular tracking - Trimming A by 20 35, reference by 20 20 Tracking image shifted 0.013, 0.149 um; position 0.084, 0.083 Drift reached 3.4 A/sec after 5.3 sec, going on Running script # 14 Trimming A by 20 20 20 35, reference by 20 20 20 35 Trimming A by 20 25 20 30, reference by 20 20 20 35 -0.3 4.3 unbinned pixels; 1.5 0.4 nm along two shift axes; -1.5 -0.3 nm on specimen axes Rate = 7.604 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_040_28.0_Oct22_11.41.38.tif Record image shifted -0.000, -0.006 um Tracking reference shifted by -16.4,1.7 pixels, -0.000,-0.006 um Saved Z = 40 at 25.63 min Tilt = 28.00 Mean = 108 Exp = 1.762 X/Y/Z = 0.087 0.090 2.299 Errors in X/Y Prediction -0.010 -0.143 um, 0.8 10.9 % Predicted X = 0.099 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.083 StdErr = 0.147 nFit = 5 nDrop = 0 Predicted Z = 2.184 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 30 30, reference by 20 20 Tracking image shifted 0.013, -0.005 um; position 0.087, 0.088 Drift reached 4.5 A/sec after 5.5 sec, going on Measured defocus = -4.60 microns changed by 0.10 to target drift = 0.27 nm/sec Trimming A by 25 30, reference by 20 20 Tracking image shifted -0.002, -0.010 um; position 0.089, 0.101 Running script # 14 Trimming A by 20 20 30 35, reference by 20 20 30 35 Trimming A by 20 20 20 35, reference by 20 20 30 35 -7.8 3.1 unbinned pixels; 1.9 2.2 nm along two shift axes; -0.8 -2.8 nm on specimen axes Rate = 14.75335 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_041_30.0_Oct22_11.42.26.tif Record image shifted 0.000, -0.012 um Tracking reference shifted by -33.4,2.6 pixels, 0.000,-0.012 um Saved Z = 41 at 26.45 min Tilt = 30.00 Mean = 107 Exp = 1.795 X/Y/Z = 0.090 0.116 2.276 Errors in X/Y Prediction -0.010 0.033 um, 0.7 2.5 % Error in Z Prediction 0.091 Predicted X = 0.089 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.017 StdErr = 0.112 nFit = 5 nDrop = 0 Predicted Z = 2.160 StdErr = 0.077 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 40, reference by 25 35 Tracking image shifted 0.005, -0.032 um; position 0.085, 0.049 Drift reached 2.6 A/sec after 5.6 sec, going on Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.14 nm/sec Trimming A by 20 35, reference by 25 35 Tracking image shifted -0.006, -0.003 um; position 0.092, 0.052 Running script # 14 Trimming A by 20 20 20 25, reference by 20 20 20 25 Trimming A by 20 20 20 25, reference by 20 20 20 25 -0.7 2.2 unbinned pixels; 0.8 0.0 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.10875 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_042_32.0_Oct22_11.43.11.tif Record image shifted -0.001, -0.006 um Tracking reference shifted by -15.8,3.1 pixels, -0.001,-0.006 um Saved Z = 42 at 27.19 min Tilt = 32.00 Mean = 108 Exp = 1.839 X/Y/Z = 0.093 0.058 2.168 Errors in X/Y Prediction 0.004 0.041 um, 0.3 3.1 % Error in Z Prediction 0.007 Predicted X = 0.076 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -1.433 StdErr = 0.024 nFit = 5 nDrop = 0 Predicted Z = 4.642 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 30, reference by 20 20 Tracking image shifted -0.028, -0.003 um; position 0.105, -1.430 Trimming A by 30 20 20 20, reference by 30 20 20 20 Drift reached 1.7 A/sec after 5.9 sec, going on Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.09 nm/sec Tracking image shifted -0.002, 0.006 um; position 0.107, -1.437 Running script # 14 Trimming A by 30 20 25 20, reference by 30 20 25 20 Trimming A by 30 20 25 20, reference by 30 20 25 20 1.3 0.6 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.3 0.4 nm on specimen axes Rate = 2.48141 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_043_-54.0_Oct22_11.44.01.tif Record image shifted 0.001, 0.012 um Tracking reference shifted by 35.0,-5.9 pixels, 0.001,0.012 um Saved Z = 43 at 28.04 min Tilt = -54.00 Mean = 105 Exp = 2.175 X/Y/Z = 0.106 -1.450 4.686 Errors in X/Y Prediction 0.030 -0.016 um, 2.3 1.2 % Error in Z Prediction 0.044 Predicted X = 0.115 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Y = -1.526 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 4.687 StdErr = 0.047 nFit = 3 nDrop = 0 Need regular tracking - Trimming A by 20 20, reference by 25 25 Tracking image shifted 0.018, 0.032 um; position 0.096, -1.559 Trimming A by 50 20 25 20, reference by 50 20 25 20 Drift reached 1.1 A/sec after 6.2 sec, going on Running script # 14 Trimming A by 30 20 30 20, reference by 30 20 30 20 Trimming A by 31 20 30 20, reference by 30 20 30 20 0.3 1.7 unbinned pixels; 0.5 0.3 nm along two shift axes; -0.6 0.0 nm on specimen axes Rate = 3.0493 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_044_-56.0_Oct22_11.44.33.tif Record image shifted 0.001, 0.012 um Tracking reference shifted by 33.0,-4.6 pixels, 0.001,0.012 um Saved Z = 44 at 28.57 min Tilt = -56.00 Mean = 105 Exp = 2.278 X/Y/Z = 0.097 -1.570 4.687 Errors in X/Y Prediction -0.018 -0.044 um, 1.4 3.4 % Predicted X = 0.114 StdErr = 0.029 nFit = 3 nDrop = 0 Predicted Y = -1.700 StdErr = 0.015 nFit = 5 nDrop = 0 Predicted Z = 4.701 StdErr = 0.055 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 25 35, reference by 25 25 Tracking image shifted 0.019, -0.066 um; position 0.094, -1.634 Drift reached 1.2 A/sec after 6.1 sec, going on Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.05 nm/sec Trimming A by 25 30, reference by 25 25 Tracking image shifted -0.001, 0.001 um; position 0.096, -1.636 Running script # 14 Trimming A by 40 20 35 20, reference by 40 20 35 20 Trimming A by 40 20 35 20, reference by 40 20 35 20 0.4 0.6 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.27621 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_045_-58.0_Oct22_11.45.18.tif Record image shifted -0.000, 0.012 um Tracking reference shifted by 34.1,-1.8 pixels, -0.000,0.012 um Saved Z = 45 at 29.32 min Tilt = -58.00 Mean = 105 Exp = 2.396 X/Y/Z = 0.096 -1.648 4.599 Errors in X/Y Prediction -0.017 0.052 um, 1.3 4.0 % Error in Z Prediction -0.101 Predicted X = 0.090 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -1.758 StdErr = 0.033 nFit = 5 nDrop = 0 Predicted Z = 4.619 StdErr = 0.097 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 50, reference by 20 45 Tracking image shifted -0.002, -0.023 um; position 0.092, -1.736 Drift reached 0.4 A/sec after 6.3 sec, going on Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.04 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.01 nm/sec Trimming A by 25 45, reference by 20 45 Tracking image shifted -0.003, 0.001 um; position 0.094, -1.736 Running script # 14 Trimming A by 40 20 45 20, reference by 40 20 45 20 Trimming A by 40 20 45 20, reference by 40 20 45 20 -0.0 0.8 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.0 nm on specimen axes Rate = 1.4884 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_046_-60.0_Oct22_11.46.14.tif Record image shifted 0.000, 0.011 um Tracking reference shifted by 31.5,-3.0 pixels, 0.000,0.011 um Saved Z = 46 at 30.25 min Tilt = -60.00 Mean = 103 Exp = 2.522 X/Y/Z = 0.095 -1.747 4.517 Errors in X/Y Prediction 0.005 0.011 um, 0.4 0.9 % Error in Z Prediction -0.102 Predicted X = 0.094 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -1.840 StdErr = 0.031 nFit = 5 nDrop = 0 Predicted Z = 4.538 StdErr = 0.089 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 25 50, reference by 25 50 Tracking image shifted 0.003, 0.011 um; position 0.091, -1.851 Drift reached 0.2 A/sec after 6.5 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.03 nm/sec Trimming A by 20 55, reference by 25 50 Tracking image shifted -0.001, 0.001 um; position 0.092, -1.852 Running script # 14 Trimming A by 45 21 50 20, reference by 45 21 50 20 Trimming A by 45 21 50 20, reference by 45 21 50 20 0.2 0.1 unbinned pixels; 0.0 0.1 nm along two shift axes; -0.1 0.1 nm on specimen axes Rate = 0.45083 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_047_-62.0_Oct22_11.47.01.tif Record image shifted 0.000, 0.013 um Tracking reference shifted by 35.5,-4.1 pixels, 0.000,0.013 um Saved Z = 47 at 31.05 min Tilt = -62.00 Mean = 102 Exp = 2.691 X/Y/Z = 0.091 -1.865 4.494 Errors in X/Y Prediction -0.002 -0.024 um, 0.2 1.9 % Error in Z Prediction -0.044 Predicted X = 0.101 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Y = 0.009 StdErr = 0.102 nFit = 5 nDrop = 0 Predicted Z = 2.071 StdErr = 0.049 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 30, reference by 20 35 Tracking image shifted -0.009, -0.061 um; position 0.110, 0.070 Drift reached 4.7 A/sec after 5.5 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.28 nm/sec Trimming A by 20 50, reference by 20 35 Tracking image shifted -0.001, -0.013 um; position 0.112, 0.086 Running script # 14 Trimming A by 20 20 30 30, reference by 20 20 30 30 Trimming A by 20 20 25 30, reference by 20 20 30 30 -5.4 1.3 unbinned pixels; 1.0 1.6 nm along two shift axes; -0.3 -1.9 nm on specimen axes Rate = 9.72206 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_048_34.0_Oct22_11.47.53.tif Record image shifted 0.001, -0.012 um Tracking reference shifted by -33.1,-0.5 pixels, 0.001,-0.012 um Saved Z = 48 at 31.89 min Tilt = 34.00 Mean = 106 Exp = 1.878 X/Y/Z = 0.111 0.100 2.053 Errors in X/Y Prediction 0.010 0.090 um, 0.8 6.9 % Error in Z Prediction -0.017 Predicted X = 0.133 StdErr = 0.009 nFit = 4 nDrop = 0 Predicted Y = 0.149 StdErr = 0.081 nFit = 5 nDrop = 0 Predicted Z = 1.986 StdErr = 0.040 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 20, reference by 20 50 Tracking image shifted 0.037, 0.177 um; position 0.096, -0.028 Drift reached 1.7 A/sec after 5.5 sec, going on Running script # 14 -2.2 1.7 unbinned pixels; 0.8 0.5 nm along two shift axes; -0.5 -0.8 nm on specimen axes Rate = 4.79737 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_049_36.0_Oct22_11.48.26.tif Record image shifted 0.002, -0.008 um Tracking reference shifted by -23.3,-3.6 pixels, 0.002,-0.008 um Saved Z = 49 at 32.44 min Tilt = 36.00 Mean = 107 Exp = 1.941 X/Y/Z = 0.096 -0.019 1.986 Errors in X/Y Prediction -0.037 -0.168 um, 2.8 12.8 % Predicted X = 0.103 StdErr = 0.025 nFit = 3 nDrop = 0 Predicted Y = -0.097 StdErr = 0.097 nFit = 5 nDrop = 0 Predicted Z = 1.899 StdErr = 0.046 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 35, reference by 20 50 Tracking image shifted 0.008, -0.068 um; position 0.094, -0.029 Drift reached 4.0 A/sec after 5.7 sec, going on Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.27 nm/sec Trimming A by 25 25, reference by 20 50 Tracking image shifted -0.002, -0.012 um; position 0.097, -0.015 Running script # 14 Trimming A by 20 20 30 20, reference by 20 20 30 20 Trimming A by 20 20 30 20, reference by 20 20 30 20 -4.1 3.8 unbinned pixels; 1.7 0.9 nm along two shift axes; -1.2 -1.6 nm on specimen axes Rate = 9.8174 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_050_38.0_Oct22_11.49.10.tif Record image shifted -0.002, -0.011 um Tracking reference shifted by -31.1,7.2 pixels, -0.002,-0.011 um Saved Z = 50 at 33.27 min Tilt = 38.00 Mean = 106 Exp = 1.995 X/Y/Z = 0.100 -0.002 1.941 Errors in X/Y Prediction -0.003 0.095 um, 0.2 7.3 % Error in Z Prediction 0.042 Predicted X = 0.091 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = -0.074 StdErr = 0.083 nFit = 6 nDrop = 0 Predicted Z = 1.839 StdErr = 0.050 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 25 30, reference by 20 30 Tracking image shifted -0.005, -0.010 um; position 0.096, -0.064 Drift reached 1.3 A/sec after 5.7 sec, going on Running script # 14 Trimming A by 20 20 35 20, reference by 20 20 35 20 Trimming A by 20 20 35 20, reference by 20 20 35 20 -1.8 2.0 unbinned pixels; 0.9 0.3 nm along two shift axes; -0.7 -0.7 nm on specimen axes Rate = 4.74377 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_051_40.0_Oct22_11.49.48.tif Record image shifted -0.003, -0.009 um Tracking reference shifted by -24.2,11.8 pixels, -0.003,-0.009 um Saved Z = 51 at 33.81 min Tilt = 40.00 Mean = 106 Exp = 2.065 X/Y/Z = 0.101 -0.054 1.839 Errors in X/Y Prediction 0.010 0.020 um, 0.8 1.6 % Predicted X = 0.104 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.106 StdErr = 0.111 nFit = 5 nDrop = 0 Predicted Z = 1.756 StdErr = 0.058 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 30 30, reference by 20 30 Tracking image shifted -0.001, -0.023 um; position 0.105, -0.083 Drift reached 4.5 A/sec after 5.9 sec, going on Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.22 nm/sec Trimming A by 25 30, reference by 20 30 Tracking image shifted -0.001, -0.012 um; position 0.107, -0.068 Running script # 14 Trimming A by 20 20 30 20, reference by 20 20 30 20 Trimming A by 20 20 35 20, reference by 20 20 30 20 -4.8 1.2 unbinned pixels; 1.0 1.4 nm along two shift axes; -0.3 -1.7 nm on specimen axes Rate = 8.61004 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_052_42.0_Oct22_11.50.34.tif Record image shifted 0.002, -0.012 um Tracking reference shifted by -35.2,-3.7 pixels, 0.002,-0.012 um Saved Z = 52 at 34.59 min Tilt = 42.00 Mean = 105 Exp = 2.141 X/Y/Z = 0.105 -0.054 1.780 Errors in X/Y Prediction 0.001 0.052 um, 0.1 4.0 % Error in Z Prediction 0.024 Predicted X = 0.089 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -1.965 StdErr = 0.035 nFit = 5 nDrop = 0 Predicted Z = 4.436 StdErr = 0.025 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 35 65, reference by 35 55 Tracking image shifted -0.018, 0.010 um; position 0.107, -1.975 Drift reached 1.8 A/sec after 6.9 sec, going on Running script # 14 Trimming A by 55 21 60 20, reference by 55 21 60 20 Trimming A by 55 21 60 20, reference by 55 21 60 20 3.2 0.3 unbinned pixels; 0.3 1.1 nm along two shift axes; -0.2 1.1 nm on specimen axes Rate = 5.63812 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_053_-64.0_Oct22_11.51.15.tif Record image shifted -0.001, 0.017 um Tracking reference shifted by 49.7,-0.5 pixels, -0.001,0.017 um Saved Z = 53 at 35.28 min Tilt = -64.00 Mean = 101 Exp = 2.891 X/Y/Z = 0.109 -1.995 4.436 Errors in X/Y Prediction 0.020 -0.030 um, 1.5 2.3 % Predicted X = 0.131 StdErr = 0.015 nFit = 4 nDrop = 0 Predicted Y = -2.147 StdErr = 0.004 nFit = 5 nDrop = 1 Predicted Z = 4.386 StdErr = 0.029 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 65, reference by 35 55 Tracking image shifted 0.034, -0.044 um; position 0.097, -2.103 Drift reached 0.8 A/sec after 7.1 sec, going on Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.04 nm/sec Trimming A by 23 75, reference by 35 55 Tracking image shifted -0.002, -0.001 um; position 0.099, -2.103 Running script # 14 Trimming A by 35 20 75 20, reference by 35 20 75 20 Trimming A by 35 20 75 20, reference by 35 20 75 20 -0.3 0.6 unbinned pixels; 0.2 0.0 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.16967 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_054_-66.0_Oct22_11.52.06.tif Record image shifted 0.002, 0.010 um Tracking reference shifted by 28.9,-7.7 pixels, 0.002,0.010 um Saved Z = 54 at 36.13 min Tilt = -66.00 Mean = 100 Exp = 3.134 X/Y/Z = 0.097 -2.113 4.423 Errors in X/Y Prediction -0.034 0.034 um, 2.6 2.6 % Error in Z Prediction 0.037 Predicted X = 0.094 StdErr = 0.034 nFit = 4 nDrop = 0 Predicted Y = -2.264 StdErr = 0.017 nFit = 6 nDrop = 0 Predicted Z = 4.365 StdErr = 0.027 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 24 95, reference by 26 80 Tracking image shifted -0.000, -0.084 um; position 0.094, -2.180 Drift reached 1.1 A/sec after 7.6 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.03 nm/sec Trimming A by 30 80, reference by 26 80 Tracking image shifted -0.000, -0.001 um; position 0.095, -2.180 Running script # 14 Trimming A by 41 21 85 20, reference by 41 21 85 20 Trimming A by 50 23 80 20, reference by 41 21 85 20 -0.1 -0.1 unbinned pixels; 0.0 0.0 nm along two shift axes; 0.0 -0.0 nm on specimen axes Rate = 0.20323 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_055_-68.0_Oct22_11.52.58.tif Record image shifted 0.000, 0.015 um Tracking reference shifted by 43.3,-4.0 pixels, 0.000,0.015 um Saved Z = 55 at 37.02 min Tilt = -68.00 Mean = 99 Exp = 3.454 X/Y/Z = 0.095 -2.195 4.360 Errors in X/Y Prediction 0.001 0.069 um, 0.1 5.3 % Error in Z Prediction -0.005 Predicted X = 0.107 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.024 StdErr = 0.079 nFit = 5 nDrop = 0 Predicted Z = 1.708 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 30, reference by 20 30 Tracking image shifted 0.022, 0.050 um; position 0.085, -0.074 Drift reached 2.2 A/sec after 6.0 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.09 nm/sec Trimming A by 20 35, reference by 20 30 Tracking image shifted -0.001, -0.004 um; position 0.087, -0.069 Running script # 14 Trimming A by 20 20 35 20, reference by 20 20 35 20 Trimming A by 20 20 40 20, reference by 20 20 35 20 -1.1 1.5 unbinned pixels; 0.6 0.2 nm along two shift axes; -0.5 -0.4 nm on specimen axes Rate = 3.2722 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_056_44.0_Oct22_11.53.54.tif Record image shifted -0.000, -0.009 um Tracking reference shifted by -24.7,2.8 pixels, -0.000,-0.009 um Saved Z = 56 at 37.92 min Tilt = 44.00 Mean = 105 Exp = 2.233 X/Y/Z = 0.088 -0.060 1.681 Errors in X/Y Prediction -0.019 -0.036 um, 1.5 2.7 % Error in Z Prediction -0.026 Predicted X = 0.085 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = -0.075 StdErr = 0.048 nFit = 5 nDrop = 0 Predicted Z = 1.623 StdErr = 0.031 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 20, reference by 20 35 Tracking image shifted 0.000, 0.092 um; position 0.084, -0.167 Drift reached 1.8 A/sec after 6.2 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.08 nm/sec Trimming A by 21 50, reference by 20 35 Tracking image shifted -0.000, -0.004 um; position 0.086, -0.163 Running script # 14 Trimming A by 20 20 50 20, reference by 20 20 50 20 Trimming A by 20 20 50 20, reference by 20 20 50 20 -1.6 1.4 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.4 -0.6 nm on specimen axes Rate = 3.70072 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_057_46.0_Oct22_11.54.42.tif Record image shifted 0.000, -0.011 um Tracking reference shifted by -30.2,2.1 pixels, 0.000,-0.011 um Saved Z = 57 at 38.72 min Tilt = 46.00 Mean = 104 Exp = 2.340 X/Y/Z = 0.086 -0.152 1.620 Errors in X/Y Prediction 0.001 -0.077 um, 0.1 5.9 % Error in Z Prediction -0.003 Predicted X = 0.074 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -0.199 StdErr = 0.073 nFit = 5 nDrop = 0 Predicted Z = 1.530 StdErr = 0.016 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 55, reference by 20 50 Tracking image shifted -0.018, -0.033 um; position 0.092, -0.166 Drift reached 3.2 A/sec after 6.3 sec, going on Running script # 14 Trimming A by 20 20 40 20, reference by 20 20 40 20 Trimming A by 20 20 40 20, reference by 20 20 40 20 -6.7 2.3 unbinned pixels; 1.6 1.9 nm along two shift axes; -0.6 -2.4 nm on specimen axes Rate = 12.36336 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_058_48.0_Oct22_11.55.18.tif Record image shifted -0.001, -0.020 um Tracking reference shifted by -55.8,7.1 pixels, -0.001,-0.020 um Saved Z = 58 at 39.32 min Tilt = 48.00 Mean = 104 Exp = 2.465 X/Y/Z = 0.095 -0.142 1.530 Errors in X/Y Prediction 0.021 0.057 um, 1.6 4.4 % Predicted X = 0.096 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.207 StdErr = 0.078 nFit = 5 nDrop = 0 Predicted Z = 1.448 StdErr = 0.018 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 25 35, reference by 20 50 Tracking image shifted 0.002, -0.009 um; position 0.094, -0.198 Drift reached 0.9 A/sec after 6.5 sec, going on Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.10 nm/sec Trimming A by 25 40, reference by 20 50 Tracking image shifted -0.003, -0.002 um; position 0.098, -0.196 Running script # 14 Trimming A by 20 20 45 20, reference by 20 20 45 20 Trimming A by 20 20 45 20, reference by 20 20 45 20 -0.9 1.2 unbinned pixels; 0.5 0.1 nm along two shift axes; -0.4 -0.3 nm on specimen axes Rate = 2.57613 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo1_L1G1_059_50.0_Oct22_11.56.07.tif Record image shifted 0.001, -0.005 um Tracking reference shifted by -14.4,-0.4 pixels, 0.001,-0.005 um Saved Z = 59 at 40.15 min Tilt = 50.00 Mean = 104 Exp = 2.613 X/Y/Z = 0.098 -0.191 1.369 2020/10/22 11:57 D:\Users\Ben\201022_Jans_cells\SEM\tomo1_L1G1.st 0 60 3838 3710 1708 -10.0 50.0 2.00 REG 40.2 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Measured defocus = -7.27 microns changed by 3.27 to target drift = 0.10 nm/sec Need regular tracking - Need autofocus Tracking image shifted 0.003, 0.009 um; position 0.021, 0.183 Drift reached 0.7 A/sec after 5.2 sec, going on Measured defocus = -3.64 microns changed by -0.36 to target drift = 0.05 nm/sec Tracking image shifted 0.001, 0.003 um; position 0.020, 0.180 Running script # 14 0.7 -0.2 unbinned pixels; 0.1 0.2 nm along two shift axes; 0.0 0.3 nm on specimen axes Rate = 1.31693 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_000_-10.0_Oct22_12.09.10.tif Record image shifted -0.000, -0.003 um Tracking reference shifted by -7.8,1.1 pixels, -0.000,-0.003 um Saved Z = 0 at 0.00 min Tilt = -10.00 Mean = 107 Exp = 1.560 X/Y/Z = 0.020 0.182 3.784 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.034 um; position 0.015, 0.216 Drift reached 0.4 A/sec after 5.2 sec, going on Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.05 nm/sec Tracking image shifted -0.000, -0.003 um; position 0.015, 0.219 Running script # 14 -0.7 0.1 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.0 -0.2 nm on specimen axes Rate = 1.21541 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_001_-12.0_Oct22_12.09.52.tif Record image shifted 0.000, 0.006 um Tracking reference shifted by 17.4,-1.9 pixels, 0.000,0.006 um Saved Z = 1 at 0.70 min Tilt = -12.00 Mean = 110 Exp = 1.599 X/Y/Z = 0.015 0.213 3.863 Predicted X = 0.010 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.245 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.942 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.008, -0.004 um; position 0.018, 0.248 Drift reached 0.2 A/sec after 5.1 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.01 nm/sec Tracking image shifted -0.000, -0.000 um; position 0.018, 0.249 Running script # 14 -0.1 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; 0.0 -0.0 nm on specimen axes Rate = 0.16882 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_002_-14.0_Oct22_12.10.33.tif Record image shifted 0.000, 0.009 um Tracking reference shifted by 25.5,-3.1 pixels, 0.000,0.009 um Saved Z = 2 at 1.38 min Tilt = -14.00 Mean = 110 Exp = 1.604 X/Y/Z = 0.018 0.240 3.949 Errors in X/Y Prediction 0.008 -0.005 um, 0.6 0.4 % Error in Z Prediction 0.007 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.015 um; position 0.016, 0.197 Drift reached 2.1 A/sec after 5.1 sec, going on Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.10 nm/sec Tracking image shifted 0.000, 0.006 um; position 0.016, 0.190 Running script # 14 1.3 -0.2 unbinned pixels; 0.2 0.4 nm along two shift axes; 0.0 0.5 nm on specimen axes Rate = 2.2868 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_003_-8.0_Oct22_12.11.17.tif Record image shifted 0.001, -0.012 um Tracking reference shifted by -35.7,-0.5 pixels, 0.001,-0.012 um Saved Z = 3 at 2.11 min Tilt = -8.00 Mean = 110 Exp = 1.599 X/Y/Z = 0.015 0.202 3.710 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.006 um; position 0.011, 0.208 Drift reached 1.6 A/sec after 5.1 sec, going on Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.09 nm/sec Tracking image shifted -0.000, -0.004 um; position 0.011, 0.213 Running script # 14 -1.1 -0.0 unbinned pixels; 0.1 0.4 nm along two shift axes; 0.1 -0.4 nm on specimen axes Rate = 1.94401 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_004_-6.0_Oct22_12.12.00.tif Record image shifted 0.000, -0.011 um Tracking reference shifted by -30.5,2.0 pixels, 0.000,-0.011 um Saved Z = 4 at 2.83 min Tilt = -6.00 Mean = 110 Exp = 1.596 X/Y/Z = 0.011 0.224 3.642 Predicted X = 0.015 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = 0.269 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Z = 4.031 StdErr = 0.005 nFit = 3 nDrop = 0 Drift reached 0.6 A/sec after 4.8 sec, going on Running script # 14 -1.9 0.2 unbinned pixels; 0.3 0.6 nm along two shift axes; -0.0 -0.7 nm on specimen axes Rate = 3.41838 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_005_-16.0_Oct22_12.12.32.tif Record image shifted -0.009, -0.109 um Saved Z = 5 at 3.35 min Tilt = -16.00 Mean = 109 Exp = 1.609 X/Y/Z = 0.025 0.378 4.031 Errors in X/Y Prediction 0.009 0.109 um, 0.7 8.4 % Predicted X = 0.029 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.442 StdErr = 0.084 nFit = 3 nDrop = 0 Predicted Z = 4.114 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.007 um; position 0.030, 0.449 Drift reached 0.7 A/sec after 5.2 sec, going on Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.04 nm/sec Tracking image shifted -0.000, 0.002 um; position 0.031, 0.447 Running script # 14 0.9 0.3 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.72934 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_006_-18.0_Oct22_12.13.13.tif Record image shifted 0.001, 0.019 um Tracking reference shifted by 53.8,-8.5 pixels, 0.001,0.019 um Saved Z = 6 at 4.05 min Tilt = -18.00 Mean = 109 Exp = 1.620 X/Y/Z = 0.029 0.427 4.201 Errors in X/Y Prediction 0.000 -0.015 um, 0.0 1.1 % Error in Z Prediction 0.087 Predicted X = 0.007 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.246 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.574 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.006, 0.002 um; position 0.013, 0.243 Drift reached 3.2 A/sec after 5.1 sec, going on Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.16 nm/sec Tracking image shifted 0.000, 0.007 um; position 0.013, 0.234 Running script # 14 2.0 -0.3 unbinned pixels; 0.3 0.6 nm along two shift axes; 0.0 0.7 nm on specimen axes Rate = 3.47166 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_007_-4.0_Oct22_12.14.03.tif Record image shifted 0.000, -0.007 um Tracking reference shifted by -20.5,0.7 pixels, 0.000,-0.007 um Saved Z = 7 at 4.88 min Tilt = -4.00 Mean = 110 Exp = 1.600 X/Y/Z = 0.013 0.241 3.573 Errors in X/Y Prediction 0.005 -0.005 um, 0.4 0.4 % Error in Z Prediction -0.001 Predicted X = 0.011 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.261 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Z = 3.504 StdErr = 0.001 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, 0.017 um; position 0.009, 0.244 Drift reached 2.3 A/sec after 5.1 sec, going on Running script # 14 -3.6 0.5 unbinned pixels; 0.6 1.1 nm along two shift axes; -0.1 -1.3 nm on specimen axes Rate = 6.33695 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_008_-2.0_Oct22_12.14.35.tif Record image shifted -0.000, -0.013 um Tracking reference shifted by -37.0,3.4 pixels, -0.000,-0.013 um Saved Z = 8 at 5.42 min Tilt = -2.00 Mean = 110 Exp = 1.598 X/Y/Z = 0.009 0.259 3.504 Errors in X/Y Prediction -0.002 -0.001 um, 0.1 0.1 % Predicted X = 0.035 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.536 StdErr = 0.067 nFit = 3 nDrop = 0 Predicted Z = 4.309 StdErr = 0.036 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, 0.224 um; position 0.028, 0.311 Drift reached 1.7 A/sec after 5.0 sec, going on Running script # 14 -0.9 0.9 unbinned pixels; 0.4 0.2 nm along two shift axes; -0.3 -0.3 nm on specimen axes Rate = 2.14649 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_009_-20.0_Oct22_12.15.07.tif Record image shifted 0.005, 0.008 um Tracking reference shifted by 20.9,-15.8 pixels, 0.005,0.008 um Saved Z = 9 at 5.95 min Tilt = -20.00 Mean = 109 Exp = 1.628 X/Y/Z = 0.025 0.304 4.309 Errors in X/Y Prediction -0.011 -0.232 um, 0.8 17.7 % Predicted X = 0.016 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = 0.071 StdErr = 0.054 nFit = 4 nDrop = 0 Predicted Z = 4.424 StdErr = 0.043 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 25, reference by 20 20 Tracking image shifted 0.003, -0.186 um; position 0.012, 0.257 Drift reached 1.7 A/sec after 5.3 sec, going on Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.05 nm/sec Tracking image shifted -0.002, -0.000 um; position 0.015, 0.257 Running script # 14 0.8 0.1 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.0 0.3 nm on specimen axes Rate = 1.4945 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_010_-22.0_Oct22_12.15.50.tif Record image shifted -0.004, 0.015 um Tracking reference shifted by 42.8,7.9 pixels, -0.004,0.015 um Saved Z = 10 at 6.65 min Tilt = -22.00 Mean = 109 Exp = 1.642 X/Y/Z = 0.019 0.242 4.338 Errors in X/Y Prediction 0.003 0.171 um, 0.3 13.1 % Error in Z Prediction -0.086 Predicted X = 0.009 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.277 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Z = 3.435 StdErr = 0.001 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.010, 0.062 um; position -0.001, 0.215 Drift reached 1.6 A/sec after 5.1 sec, going on Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.06 nm/sec Tracking image shifted 0.000, 0.002 um; position -0.001, 0.212 Running script # 14 0.6 0.5 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.44832 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_011_-0.0_Oct22_12.16.36.tif Record image shifted 0.001, -0.005 um Tracking reference shifted by -15.3,-2.1 pixels, 0.001,-0.005 um Saved Z = 11 at 7.43 min Tilt = -0.00 Mean = 109 Exp = 1.604 X/Y/Z = -0.002 0.217 3.473 Errors in X/Y Prediction -0.010 -0.060 um, 0.8 4.6 % Error in Z Prediction 0.037 Predicted X = -0.008 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = 0.215 StdErr = 0.046 nFit = 3 nDrop = 0 Predicted Z = 3.415 StdErr = 0.017 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.008, 0.007 um; position 0.000, 0.209 Drift reached 2.5 A/sec after 5.3 sec, going on Running script # 14 -4.9 0.9 unbinned pixels; 0.9 1.5 nm along two shift axes; -0.2 -1.7 nm on specimen axes Rate = 8.66028 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_012_2.0_Oct22_12.17.09.tif Record image shifted -0.000, -0.012 um Tracking reference shifted by -33.0,4.0 pixels, -0.000,-0.012 um Saved Z = 12 at 7.98 min Tilt = 2.00 Mean = 110 Exp = 1.614 X/Y/Z = 0.001 0.224 3.415 Errors in X/Y Prediction 0.008 0.008 um, 0.6 0.6 % Predicted X = 0.014 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.010 StdErr = 0.101 nFit = 5 nDrop = 0 Predicted Z = 4.454 StdErr = 0.073 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 25, reference by 20 20 Tracking image shifted -0.005, -0.134 um; position 0.019, 0.144 Drift reached 1.7 A/sec after 5.3 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.05 nm/sec Tracking image shifted -0.002, -0.002 um; position 0.022, 0.146 Running script # 14 -0.1 0.5 unbinned pixels; 0.2 0.0 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 0.95939 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_013_-24.0_Oct22_12.17.54.tif Record image shifted 0.000, 0.010 um Tracking reference shifted by 27.4,-3.8 pixels, 0.000,0.010 um Saved Z = 13 at 8.73 min Tilt = -24.00 Mean = 109 Exp = 1.654 X/Y/Z = 0.022 0.136 4.407 Errors in X/Y Prediction 0.008 0.126 um, 0.6 9.6 % Error in Z Prediction -0.047 Predicted X = 0.019 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.028 StdErr = 0.095 nFit = 5 nDrop = 0 Predicted Z = 4.476 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 25 25, reference by 20 20 Tracking image shifted 0.003, -0.113 um; position 0.017, 0.085 Drift reached 1.3 A/sec after 5.4 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.07 nm/sec Tracking image shifted -0.002, 0.001 um; position 0.019, 0.083 Running script # 14 0.9 0.5 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.87937 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_014_-26.0_Oct22_12.18.37.tif Record image shifted 0.000, 0.014 um Tracking reference shifted by 39.9,-4.1 pixels, 0.000,0.014 um Saved Z = 14 at 9.44 min Tilt = -26.00 Mean = 109 Exp = 1.670 X/Y/Z = 0.019 0.069 4.500 Errors in X/Y Prediction -0.001 0.096 um, 0.0 7.4 % Error in Z Prediction 0.025 Predicted X = 0.001 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Y = 0.197 StdErr = 0.073 nFit = 4 nDrop = 0 Predicted Z = 3.359 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, -0.049 um; position 0.001, 0.245 Drift reached 3.0 A/sec after 5.1 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.14 nm/sec Tracking image shifted -0.002, 0.004 um; position 0.004, 0.239 Running script # 14 1.1 0.8 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.3 0.4 nm on specimen axes Rate = 2.38417 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_015_4.0_Oct22_12.19.23.tif Record image shifted 0.001, -0.003 um Tracking reference shifted by -8.6,-2.9 pixels, 0.001,-0.003 um Saved Z = 15 at 10.30 min Tilt = 4.00 Mean = 109 Exp = 1.620 X/Y/Z = 0.003 0.242 3.318 Errors in X/Y Prediction 0.001 0.045 um, 0.1 3.5 % Error in Z Prediction -0.042 Predicted X = 0.005 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.251 StdErr = 0.049 nFit = 5 nDrop = 0 Predicted Z = 3.263 StdErr = 0.035 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, 0.000 um; position 0.002, 0.250 Drift reached 5.0 A/sec after 5.2 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.34 nm/sec Tracking image shifted -0.002, -0.012 um; position 0.005, 0.265 Running script # 14 -5.4 1.8 unbinned pixels; 1.2 1.6 nm along two shift axes; -0.5 -1.9 nm on specimen axes Rate = 9.95327 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_016_6.0_Oct22_12.20.12.tif Record image shifted -0.000, -0.009 um Tracking reference shifted by -26.1,3.2 pixels, -0.000,-0.009 um Saved Z = 16 at 11.03 min Tilt = 6.00 Mean = 109 Exp = 1.632 X/Y/Z = 0.006 0.276 3.270 Errors in X/Y Prediction 0.001 0.025 um, 0.1 1.9 % Error in Z Prediction 0.007 Predicted X = 0.020 StdErr = 0.009 nFit = 4 nDrop = 0 Predicted Y = 0.004 StdErr = 0.039 nFit = 5 nDrop = 1 Predicted Z = 4.564 StdErr = 0.016 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 25, reference by 20 20 Tracking image shifted -0.004, 0.034 um; position 0.024, -0.030 Drift reached 1.1 A/sec after 5.4 sec, going on Running script # 14 Trimming A by 40 20 20 20, reference by 40 20 20 20 Trimming A by 40 20 20 20, reference by 40 20 20 20 0.8 0.8 unbinned pixels; 0.2 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.01045 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_017_-28.0_Oct22_12.20.46.tif Record image shifted 0.001, 0.012 um Tracking reference shifted by 34.2,-4.7 pixels, 0.001,0.012 um Saved Z = 17 at 11.59 min Tilt = -28.00 Mean = 109 Exp = 1.685 X/Y/Z = 0.024 -0.043 4.564 Errors in X/Y Prediction 0.004 -0.047 um, 0.3 3.6 % Predicted X = 0.024 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.149 StdErr = 0.033 nFit = 5 nDrop = 0 Predicted Z = 4.638 StdErr = 0.019 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.016 um; position 0.021, -0.133 Drift reached 0.7 A/sec after 5.5 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.06 nm/sec Trimming A by 20 25, reference by 20 20 Tracking image shifted -0.001, 0.002 um; position 0.022, -0.136 Running script # 14 Trimming A by 21 20 25 20, reference by 21 20 25 20 Trimming A by 21 20 25 20, reference by 21 20 25 20 1.0 0.7 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.16322 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_018_-30.0_Oct22_12.21.29.tif Record image shifted 0.000, 0.017 um Tracking reference shifted by 48.2,-5.2 pixels, 0.000,0.017 um Saved Z = 18 at 12.31 min Tilt = -30.00 Mean = 109 Exp = 1.708 X/Y/Z = 0.022 -0.153 4.591 Errors in X/Y Prediction -0.001 -0.004 um, 0.1 0.3 % Error in Z Prediction -0.046 Predicted X = 0.008 StdErr = 0.001 nFit = 4 nDrop = 0 Predicted Y = 0.343 StdErr = 0.019 nFit = 5 nDrop = 1 Predicted Z = 3.192 StdErr = 0.025 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, 0.047 um; position 0.009, 0.297 Drift reached 1.2 A/sec after 5.6 sec, going on Running script # 14 0.4 1.8 unbinned pixels; 0.6 0.3 nm along two shift axes; -0.7 0.1 nm on specimen axes Rate = 3.29677 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_019_8.0_Oct22_12.22.05.tif Record image shifted 0.001, -0.002 um Tracking reference shifted by -6.4,-2.3 pixels, 0.001,-0.002 um Saved Z = 19 at 12.92 min Tilt = 8.00 Mean = 109 Exp = 1.644 X/Y/Z = 0.009 0.298 3.192 Errors in X/Y Prediction 0.000 -0.045 um, 0.0 3.5 % Predicted X = 0.012 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.339 StdErr = 0.014 nFit = 5 nDrop = 0 Predicted Z = 3.123 StdErr = 0.031 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.011, 0.073 um; position 0.001, 0.266 Drift reached 4.2 A/sec after 5.3 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.27 nm/sec Tracking image shifted -0.001, -0.010 um; position 0.003, 0.278 Running script # 14 -5.0 2.2 unbinned pixels; 1.3 1.4 nm along two shift axes; -0.6 -1.8 nm on specimen axes Rate = 9.62649 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_020_10.0_Oct22_12.22.51.tif Record image shifted -0.001, -0.008 um Tracking reference shifted by -21.5,3.5 pixels, -0.001,-0.008 um Saved Z = 20 at 13.67 min Tilt = 10.00 Mean = 109 Exp = 1.653 X/Y/Z = 0.004 0.288 3.084 Errors in X/Y Prediction -0.007 -0.052 um, 0.6 4.0 % Error in Z Prediction -0.039 Predicted X = 0.026 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.267 StdErr = 0.032 nFit = 5 nDrop = 0 Predicted Z = 4.667 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 20, reference by 20 25 Tracking image shifted -0.004, -0.018 um; position 0.029, -0.249 Drift reached 0.4 A/sec after 5.4 sec, going on Running script # 14 0.5 1.1 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.1 nm on specimen axes Rate = 2.04953 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_021_-32.0_Oct22_12.23.25.tif Record image shifted -0.001, 0.014 um Tracking reference shifted by 41.1,-1.7 pixels, -0.001,0.014 um Saved Z = 21 at 14.25 min Tilt = -32.00 Mean = 108 Exp = 1.723 X/Y/Z = 0.031 -0.264 4.667 Errors in X/Y Prediction 0.005 0.003 um, 0.4 0.2 % Predicted X = 0.032 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.400 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 4.730 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 20, reference by 20 25 Tracking image shifted 0.003, -0.071 um; position 0.030, -0.330 Drift reached 2.0 A/sec after 5.4 sec, going on Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.09 nm/sec Trimming A by 20 20, reference by 20 25 Tracking image shifted -0.001, 0.001 um; position 0.031, -0.332 Running script # 14 1.4 0.7 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 2.70286 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_022_-34.0_Oct22_12.24.08.tif Record image shifted 0.002, 0.020 um Tracking reference shifted by 55.3,-10.0 pixels, 0.002,0.020 um Saved Z = 22 at 14.96 min Tilt = -34.00 Mean = 109 Exp = 1.756 X/Y/Z = 0.030 -0.352 4.651 Errors in X/Y Prediction -0.002 0.048 um, 0.2 3.7 % Error in Z Prediction -0.079 Predicted X = -0.002 StdErr = 0.005 nFit = 4 nDrop = 0 Predicted Y = 0.307 StdErr = 0.028 nFit = 6 nDrop = 0 Predicted Z = 3.018 StdErr = 0.027 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.010, -0.026 um; position 0.008, 0.333 Drift reached 3.6 A/sec after 5.3 sec, going on Running script # 14 -5.8 2.6 unbinned pixels; 1.5 1.6 nm along two shift axes; -0.7 -2.1 nm on specimen axes Rate = 11.11244 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_023_12.0_Oct22_12.24.45.tif Record image shifted -0.001, -0.007 um Tracking reference shifted by -20.3,4.9 pixels, -0.001,-0.007 um Saved Z = 23 at 15.57 min Tilt = 12.00 Mean = 109 Exp = 1.671 X/Y/Z = 0.010 0.345 3.018 Errors in X/Y Prediction 0.012 0.038 um, 0.9 2.9 % Predicted X = 0.013 StdErr = 0.012 nFit = 4 nDrop = 0 Predicted Y = 0.361 StdErr = 0.048 nFit = 5 nDrop = 0 Predicted Z = 2.941 StdErr = 0.031 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, 0.028 um; position 0.010, 0.333 Drift reached 1.8 A/sec after 5.3 sec, going on Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.14 nm/sec Tracking image shifted -0.002, -0.005 um; position 0.014, 0.338 Running script # 14 -1.6 1.6 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.5 -0.6 nm on specimen axes Rate = 3.99676 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_024_14.0_Oct22_12.25.29.tif Record image shifted 0.002, 0.002 um Tracking reference shifted by 3.9,-7.4 pixels, 0.002,0.002 um Saved Z = 24 at 16.31 min Tilt = 14.00 Mean = 109 Exp = 1.690 X/Y/Z = 0.012 0.337 2.866 Errors in X/Y Prediction -0.001 -0.024 um, 0.1 1.8 % Error in Z Prediction -0.075 Predicted X = 0.035 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.444 StdErr = 0.012 nFit = 5 nDrop = 1 Predicted Z = 4.714 StdErr = 0.043 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, 0.003 um; position 0.034, -0.447 Drift reached 0.5 A/sec after 5.3 sec, going on Running script # 14 0.6 1.1 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.2 nm on specimen axes Rate = 2.26542 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_025_-36.0_Oct22_12.26.05.tif Record image shifted -0.000, 0.017 um Tracking reference shifted by 47.5,-3.8 pixels, -0.000,0.017 um Saved Z = 25 at 16.91 min Tilt = -36.00 Mean = 108 Exp = 1.779 X/Y/Z = 0.035 -0.464 4.714 Errors in X/Y Prediction 0.000 -0.020 um, 0.0 1.6 % Predicted X = 0.036 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.558 StdErr = 0.019 nFit = 5 nDrop = 0 Predicted Z = 4.761 StdErr = 0.048 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, -0.083 um; position 0.034, -0.475 Drift reached 2.3 A/sec after 5.6 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.09 nm/sec Tracking image shifted -0.002, -0.001 um; position 0.036, -0.475 Running script # 14 1.1 0.8 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.35149 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_026_-38.0_Oct22_12.26.49.tif Record image shifted 0.001, 0.022 um Tracking reference shifted by 61.6,-9.0 pixels, 0.001,0.022 um Saved Z = 26 at 17.64 min Tilt = -38.00 Mean = 108 Exp = 1.813 X/Y/Z = 0.035 -0.497 4.743 Errors in X/Y Prediction -0.001 0.061 um, 0.1 4.6 % Error in Z Prediction -0.018 Predicted X = 0.016 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.354 StdErr = 0.036 nFit = 6 nDrop = 0 Predicted Z = 2.789 StdErr = 0.041 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, -0.037 um; position 0.014, 0.390 Drift reached 4.8 A/sec after 5.3 sec, going on Running script # 14 -7.2 3.1 unbinned pixels; 1.9 2.0 nm along two shift axes; -0.9 -2.6 nm on specimen axes Rate = 13.7826 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_027_16.0_Oct22_12.27.26.tif Record image shifted -0.002, -0.014 um Tracking reference shifted by -38.9,9.0 pixels, -0.002,-0.014 um Saved Z = 27 at 18.27 min Tilt = 16.00 Mean = 108 Exp = 1.704 X/Y/Z = 0.018 0.409 2.789 Errors in X/Y Prediction 0.002 0.056 um, 0.2 4.3 % Predicted X = 0.023 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = 0.453 StdErr = 0.042 nFit = 6 nDrop = 0 Predicted Z = 2.697 StdErr = 0.046 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, 0.161 um; position 0.023, 0.292 Drift reached 3.6 A/sec after 5.3 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.18 nm/sec Tracking image shifted -0.003, -0.003 um; position 0.028, 0.297 Running script # 14 -0.8 2.4 unbinned pixels; 0.9 0.0 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.37597 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_028_18.0_Oct22_12.28.11.tif Record image shifted 0.002, 0.009 um Tracking reference shifted by 26.2,-9.2 pixels, 0.002,0.009 um Saved Z = 28 at 19.00 min Tilt = 18.00 Mean = 109 Exp = 1.728 X/Y/Z = 0.027 0.287 2.666 Errors in X/Y Prediction 0.004 -0.166 um, 0.3 12.7 % Error in Z Prediction -0.031 Predicted X = 0.039 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.547 StdErr = 0.042 nFit = 5 nDrop = 0 Predicted Z = 4.775 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.000, 0.009 um; position 0.039, -0.556 Drift reached 0.5 A/sec after 5.6 sec, going on Running script # 14 0.7 1.0 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.4 0.2 nm on specimen axes Rate = 2.12405 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_029_-40.0_Oct22_12.28.46.tif Record image shifted -0.000, 0.019 um Tracking reference shifted by 54.8,-4.0 pixels, -0.000,0.019 um Saved Z = 29 at 19.59 min Tilt = -40.00 Mean = 108 Exp = 1.846 X/Y/Z = 0.040 -0.576 4.775 Errors in X/Y Prediction 0.001 -0.029 um, 0.1 2.2 % Predicted X = 0.041 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = -0.614 StdErr = 0.033 nFit = 6 nDrop = 0 Predicted Z = 4.813 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.018 um; position 0.038, -0.596 Drift reached 1.5 A/sec after 5.5 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.004 um; position 0.038, -0.601 Running script # 14 1.1 0.5 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.05663 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_030_-42.0_Oct22_12.29.29.tif Record image shifted 0.001, 0.024 um Tracking reference shifted by 68.7,-10.2 pixels, 0.001,0.024 um Saved Z = 30 at 20.32 min Tilt = -42.00 Mean = 107 Exp = 1.887 X/Y/Z = 0.037 -0.626 4.749 Errors in X/Y Prediction -0.004 -0.012 um, 0.3 0.9 % Error in Z Prediction -0.064 Predicted X = 0.039 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.661 StdErr = 0.048 nFit = 5 nDrop = 0 Predicted Z = 4.796 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, 0.021 um; position 0.031, -0.682 Trimming A by 45 20 20 20, reference by 50 20 20 20 Drift reached 0.5 A/sec after 5.6 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.03 nm/sec Tracking image shifted -0.001, -0.000 um; position 0.032, -0.681 Running script # 14 -0.0 0.8 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.0 nm on specimen axes Rate = 1.34027 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_031_-44.0_Oct22_12.30.12.tif Record image shifted 0.000, 0.022 um Tracking reference shifted by 62.4,-6.1 pixels, 0.000,0.022 um Saved Z = 31 at 21.03 min Tilt = -44.00 Mean = 107 Exp = 1.933 X/Y/Z = 0.032 -0.703 4.768 Errors in X/Y Prediction -0.008 -0.042 um, 0.6 3.3 % Error in Z Prediction -0.028 Predicted X = 0.034 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.213 StdErr = 0.108 nFit = 5 nDrop = 0 Predicted Z = 2.566 StdErr = 0.013 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.023, -0.147 um; position 0.010, 0.361 Drift reached 5.2 A/sec after 5.3 sec, going on Running script # 14 -8.1 3.8 unbinned pixels; 2.2 2.2 nm along two shift axes; -1.1 -3.0 nm on specimen axes Rate = 15.7013 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_032_20.0_Oct22_12.30.50.tif Record image shifted -0.002, -0.012 um Tracking reference shifted by -32.8,8.1 pixels, -0.002,-0.012 um Saved Z = 32 at 21.66 min Tilt = 20.00 Mean = 108 Exp = 1.752 X/Y/Z = 0.015 0.378 2.566 Errors in X/Y Prediction -0.019 0.165 um, 1.4 12.6 % Predicted X = 0.017 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = 0.328 StdErr = 0.112 nFit = 6 nDrop = 0 Predicted Z = 2.465 StdErr = 0.015 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.008, 0.080 um; position 0.024, 0.247 Drift reached 3.5 A/sec after 5.4 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.16 nm/sec Tracking image shifted -0.004, -0.005 um; position 0.030, 0.252 Running script # 14 -0.6 2.4 unbinned pixels; 0.9 0.1 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.2788 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_033_22.0_Oct22_12.31.35.tif Trimming A by 20 25, reference by 20 20 Record image shifted 0.004, 0.009 um Tracking reference shifted by 24.9,-12.3 pixels, 0.004,0.009 um Saved Z = 33 at 22.41 min Tilt = 22.00 Mean = 108 Exp = 1.779 X/Y/Z = 0.027 0.244 2.437 Errors in X/Y Prediction 0.011 -0.084 um, 0.8 6.4 % Error in Z Prediction -0.028 Predicted X = 0.024 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.165 StdErr = 0.170 nFit = 5 nDrop = 0 Predicted Z = 2.335 StdErr = 0.012 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.000, -0.099 um; position 0.024, 0.264 Drift reached 3.4 A/sec after 5.6 sec, going on Running script # 14 -6.8 2.4 unbinned pixels; 1.6 2.0 nm along two shift axes; -0.6 -2.5 nm on specimen axes Rate = 12.65727 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_034_24.0_Oct22_12.32.08.tif Trimming A by 20 25, reference by 20 25 Record image shifted -0.001, -0.007 um Tracking reference shifted by -19.2,4.2 pixels, -0.001,-0.007 um Saved Z = 34 at 22.96 min Tilt = 24.00 Mean = 108 Exp = 1.811 X/Y/Z = 0.027 0.275 2.335 Errors in X/Y Prediction 0.003 0.109 um, 0.2 8.4 % Predicted X = 0.020 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Y = -0.785 StdErr = 0.028 nFit = 5 nDrop = 1 Predicted Z = 4.797 StdErr = 0.037 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.020, -0.048 um; position 0.040, -0.738 Trimming A by 65 22 65 20, reference by 65 22 65 20 Drift reached 2.6 A/sec after 5.7 sec, going on Running script # 14 3.1 1.3 unbinned pixels; 0.1 1.2 nm along two shift axes; -0.5 1.1 nm on specimen axes Rate = 5.91673 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_035_-46.0_Oct22_12.32.45.tif Record image shifted -0.000, 0.026 um Tracking reference shifted by 74.7,-6.3 pixels, -0.000,0.026 um Saved Z = 35 at 23.58 min Tilt = -46.00 Mean = 107 Exp = 1.986 X/Y/Z = 0.042 -0.767 4.797 Errors in X/Y Prediction 0.022 0.019 um, 1.7 1.4 % Predicted X = 0.041 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.832 StdErr = 0.021 nFit = 5 nDrop = 0 Predicted Z = 4.819 StdErr = 0.041 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, 0.038 um; position 0.039, -0.870 Trimming A by 65 20 50 20, reference by 60 20 55 20 Drift reached 1.2 A/sec after 5.7 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.04 nm/sec Tracking image shifted -0.000, 0.002 um; position 0.039, -0.873 Running script # 14 0.5 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.63423 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_036_-48.0_Oct22_12.33.29.tif Record image shifted 0.002, 0.026 um Tracking reference shifted by 72.7,-10.8 pixels, 0.002,0.026 um Saved Z = 36 at 24.31 min Tilt = -48.00 Mean = 106 Exp = 2.043 X/Y/Z = 0.038 -0.899 4.849 Errors in X/Y Prediction -0.003 -0.067 um, 0.2 5.1 % Error in Z Prediction 0.030 Predicted X = 0.044 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.997 StdErr = 0.037 nFit = 6 nDrop = 0 Predicted Z = 4.851 StdErr = 0.039 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, -0.089 um; position 0.036, -0.908 Trimming A by 23 20 65 20, reference by 23 20 65 20 Drift reached 2.2 A/sec after 5.9 sec, going on Running script # 14 1.5 1.5 unbinned pixels; 0.3 0.7 nm along two shift axes; -0.6 0.5 nm on specimen axes Rate = 3.69377 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_037_-50.0_Oct22_12.34.01.tif Record image shifted -0.001, 0.027 um Tracking reference shifted by 75.4,-5.0 pixels, -0.001,0.027 um Saved Z = 37 at 24.85 min Tilt = -50.00 Mean = 106 Exp = 2.114 X/Y/Z = 0.039 -0.936 4.851 Errors in X/Y Prediction -0.005 0.061 um, 0.4 4.7 % Predicted X = 0.036 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -1.025 StdErr = 0.065 nFit = 5 nDrop = 0 Predicted Z = 4.872 StdErr = 0.044 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.041 um; position 0.037, -0.984 Drift reached 1.1 A/sec after 5.9 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.000 um; position 0.038, -0.985 Running script # 14 1.0 0.6 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 2.03205 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_038_-52.0_Oct22_12.34.46.tif Record image shifted 0.001, 0.034 um Tracking reference shifted by 96.3,-11.7 pixels, 0.001,0.034 um Saved Z = 38 at 25.60 min Tilt = -52.00 Mean = 106 Exp = 2.187 X/Y/Z = 0.037 -1.020 4.898 Errors in X/Y Prediction 0.001 0.005 um, 0.1 0.4 % Error in Z Prediction 0.026 Predicted X = 0.035 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.265 StdErr = 0.168 nFit = 5 nDrop = 0 Predicted Z = 2.228 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.032, -0.006 um; position 0.002, 0.271 Drift reached 3.1 A/sec after 5.6 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.18 nm/sec Tracking image shifted -0.002, -0.004 um; position 0.005, 0.277 Running script # 14 0.3 2.2 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.8 0.0 nm on specimen axes Rate = 3.92737 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_039_26.0_Oct22_12.35.42.tif Record image shifted 0.003, 0.004 um Tracking reference shifted by 11.4,-9.3 pixels, 0.003,0.004 um Saved Z = 39 at 26.53 min Tilt = 26.00 Mean = 108 Exp = 1.840 X/Y/Z = 0.003 0.273 2.184 Errors in X/Y Prediction -0.032 0.008 um, 2.4 0.6 % Error in Z Prediction -0.044 Predicted X = -0.006 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = 0.242 StdErr = 0.156 nFit = 5 nDrop = 0 Predicted Z = 2.068 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.008, 0.059 um; position 0.003, 0.184 Drift reached 2.7 A/sec after 5.6 sec, going on Running script # 14 -0.9 4.1 unbinned pixels; 1.4 0.2 nm along two shift axes; -1.4 -0.4 nm on specimen axes Rate = 7.30966 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_040_28.0_Oct22_12.36.16.tif Record image shifted -0.002, 0.002 um Tracking reference shifted by 5.7,5.3 pixels, -0.002,0.002 um Saved Z = 40 at 27.10 min Tilt = 28.00 Mean = 108 Exp = 1.877 X/Y/Z = 0.008 0.183 2.068 Errors in X/Y Prediction 0.013 -0.060 um, 1.0 4.6 % Predicted X = -0.007 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Y = 0.190 StdErr = 0.138 nFit = 5 nDrop = 0 Predicted Z = 1.947 StdErr = 0.017 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.019, 0.008 um; position 0.013, 0.182 Drift reached 5.3 A/sec after 7.5 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.25 nm/sec Tracking image shifted -0.003, -0.010 um; position 0.016, 0.196 Running script # 14 -4.6 1.6 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.5461 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_041_30.0_Oct22_12.37.04.tif Trimming A by 20 20, reference by 20 25 Record image shifted 0.003, 0.003 um Tracking reference shifted by 7.6,-9.6 pixels, 0.003,0.003 um Saved Z = 41 at 27.89 min Tilt = 30.00 Mean = 108 Exp = 1.921 X/Y/Z = 0.013 0.194 1.975 Errors in X/Y Prediction 0.020 0.005 um, 1.5 0.4 % Error in Z Prediction 0.028 Predicted X = 0.018 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.113 StdErr = 0.087 nFit = 5 nDrop = 0 Predicted Z = 1.852 StdErr = 0.028 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.008, -0.061 um; position 0.010, 0.173 Drift reached 2.5 A/sec after 5.8 sec, going on Running script # 14 -1.1 4.4 unbinned pixels; 1.6 0.2 nm along two shift axes; -1.5 -0.5 nm on specimen axes Rate = 7.94272 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_042_32.0_Oct22_12.37.37.tif Trimming A by 20 25, reference by 20 25 Record image shifted -0.003, 0.005 um Tracking reference shifted by 14.0,8.0 pixels, -0.003,0.005 um Saved Z = 42 at 28.46 min Tilt = 32.00 Mean = 107 Exp = 1.962 X/Y/Z = 0.016 0.169 1.852 Errors in X/Y Prediction -0.002 0.057 um, 0.1 4.3 % Predicted X = 0.037 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -1.079 StdErr = 0.062 nFit = 5 nDrop = 0 Predicted Z = 4.933 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.009, 0.046 um; position 0.046, -1.125 Drift reached 2.5 A/sec after 6.2 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.14 nm/sec Tracking image shifted -0.001, 0.005 um; position 0.048, -1.132 Running script # 14 1.6 1.2 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.5 0.5 nm on specimen axes Rate = 3.57573 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_043_-54.0_Oct22_12.38.29.tif Record image shifted 0.001, 0.029 um Tracking reference shifted by 82.9,-9.9 pixels, 0.001,0.029 um Saved Z = 43 at 29.33 min Tilt = -54.00 Mean = 105 Exp = 2.275 X/Y/Z = 0.047 -1.161 4.937 Errors in X/Y Prediction 0.010 -0.083 um, 0.8 6.3 % Error in Z Prediction 0.004 Predicted X = 0.050 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -1.262 StdErr = 0.085 nFit = 5 nDrop = 0 Predicted Z = 4.969 StdErr = 0.012 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.015, -0.003 um; position 0.035, -1.259 Drift reached 0.8 A/sec after 6.1 sec, going on Running script # 14 1.2 1.5 unbinned pixels; 0.4 0.6 nm along two shift axes; -0.6 0.4 nm on specimen axes Rate = 3.31185 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_044_-56.0_Oct22_12.39.03.tif Record image shifted -0.000, 0.034 um Tracking reference shifted by 97.2,-8.4 pixels, -0.000,0.034 um Saved Z = 44 at 29.89 min Tilt = -56.00 Mean = 105 Exp = 2.379 X/Y/Z = 0.036 -1.294 4.969 Errors in X/Y Prediction -0.014 -0.032 um, 1.1 2.5 % Predicted X = 0.038 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = -1.492 StdErr = 0.033 nFit = 5 nDrop = 0 Predicted Z = 5.002 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.121 um; position 0.033, -1.371 Drift reached 1.3 A/sec after 6.3 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.07 nm/sec Tracking image shifted -0.003, -0.004 um; position 0.036, -1.368 Running script # 14 1.1 1.0 unbinned pixels; 0.2 0.5 nm along two shift axes; -0.4 0.3 nm on specimen axes Rate = 2.55428 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_045_-58.0_Oct22_12.39.51.tif Record image shifted 0.001, 0.037 um Tracking reference shifted by 105.0,-12.5 pixels, 0.001,0.037 um Saved Z = 45 at 30.69 min Tilt = -58.00 Mean = 104 Exp = 2.490 X/Y/Z = 0.036 -1.405 4.954 Errors in X/Y Prediction -0.003 0.087 um, 0.2 6.6 % Error in Z Prediction -0.048 Predicted X = 0.028 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -1.550 StdErr = 0.044 nFit = 5 nDrop = 0 Predicted Z = 4.986 StdErr = 0.021 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.003, -0.058 um; position 0.031, -1.492 Drift reached 1.6 A/sec after 6.6 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.07 nm/sec Tracking image shifted -0.002, -0.002 um; position 0.035, -1.490 Running script # 14 0.5 1.2 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.1 nm on specimen axes Rate = 2.25393 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_046_-60.0_Oct22_12.40.40.tif Record image shifted 0.001, 0.037 um Tracking reference shifted by 105.8,-10.9 pixels, 0.001,0.037 um Saved Z = 46 at 31.51 min Tilt = -60.00 Mean = 103 Exp = 2.623 X/Y/Z = 0.035 -1.528 4.939 Errors in X/Y Prediction 0.006 0.022 um, 0.5 1.7 % Error in Z Prediction -0.047 Predicted X = 0.034 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -1.630 StdErr = 0.014 nFit = 5 nDrop = 0 Predicted Z = 4.962 StdErr = 0.028 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.043 um; position 0.031, -1.587 Drift reached 1.0 A/sec after 6.8 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.05 nm/sec Tracking image shifted -0.002, -0.001 um; position 0.033, -1.587 Running script # 14 0.6 0.9 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.88263 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_047_-62.0_Oct22_12.41.30.tif Record image shifted 0.001, 0.033 um Tracking reference shifted by 92.3,-10.3 pixels, 0.001,0.033 um Saved Z = 47 at 32.35 min Tilt = -62.00 Mean = 103 Exp = 2.795 X/Y/Z = 0.032 -1.620 4.946 Errors in X/Y Prediction -0.002 0.010 um, 0.1 0.7 % Error in Z Prediction -0.015 Predicted X = 0.021 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.160 StdErr = 0.071 nFit = 5 nDrop = 0 Predicted Z = 1.737 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.019, -0.058 um; position 0.040, 0.218 Drift reached 5.5 A/sec after 5.7 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.32 nm/sec Tracking image shifted -0.002, -0.014 um; position 0.043, 0.235 Running script # 14 -5.8 1.8 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 10.62251 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_048_34.0_Oct22_12.42.25.tif Record image shifted 0.005, 0.000 um Tracking reference shifted by -1.1,-15.1 pixels, 0.005,0.000 um Saved Z = 48 at 33.24 min Tilt = 34.00 Mean = 107 Exp = 2.018 X/Y/Z = 0.038 0.237 1.691 Errors in X/Y Prediction 0.017 0.077 um, 1.3 5.9 % Error in Z Prediction -0.046 Predicted X = 0.048 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.326 StdErr = 0.054 nFit = 5 nDrop = 0 Predicted Z = 1.580 StdErr = 0.048 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.021, 0.190 um; position 0.027, 0.136 Drift reached 2.9 A/sec after 5.6 sec, going on Running script # 14 -2.5 3.1 unbinned pixels; 1.3 0.5 nm along two shift axes; -1.0 -1.0 nm on specimen axes Rate = 7.03804 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_049_36.0_Oct22_12.42.59.tif Record image shifted 0.003, 0.006 um Tracking reference shifted by 15.7,-10.2 pixels, 0.003,0.006 um Saved Z = 49 at 33.82 min Tilt = 36.00 Mean = 106 Exp = 2.078 X/Y/Z = 0.026 0.132 1.580 Errors in X/Y Prediction -0.022 -0.194 um, 1.7 14.9 % Predicted X = 0.037 StdErr = 0.025 nFit = 3 nDrop = 0 Predicted Y = 0.095 StdErr = 0.107 nFit = 5 nDrop = 0 Predicted Z = 1.457 StdErr = 0.055 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, -0.012 um; position 0.027, 0.107 Drift reached 4.4 A/sec after 6.0 sec, going on Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.26 nm/sec Tracking image shifted -0.003, -0.012 um; position 0.030, 0.121 Running script # 14 -1.8 2.7 unbinned pixels; 1.1 0.3 nm along two shift axes; -0.9 -0.7 nm on specimen axes Rate = 5.7617 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_050_38.0_Oct22_12.43.47.tif Record image shifted -0.002, 0.005 um Tracking reference shifted by 13.6,4.3 pixels, -0.002,0.005 um Saved Z = 50 at 34.61 min Tilt = 38.00 Mean = 106 Exp = 2.147 X/Y/Z = 0.033 0.117 1.512 Errors in X/Y Prediction -0.003 0.023 um, 0.3 1.7 % Error in Z Prediction 0.055 Predicted X = 0.028 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.036 StdErr = 0.106 nFit = 5 nDrop = 0 Predicted Z = 1.379 StdErr = 0.067 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, 0.004 um; position 0.033, 0.032 Drift reached 1.6 A/sec after 6.0 sec, going on Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.11 nm/sec Tracking image shifted -0.004, -0.003 um; position 0.038, 0.035 Running script # 14 -1.3 1.5 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.5 -0.5 nm on specimen axes Rate = 3.55482 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_051_40.0_Oct22_12.44.35.tif Record image shifted -0.000, 0.011 um Tracking reference shifted by 30.2,-2.6 pixels, -0.000,0.011 um Saved Z = 51 at 35.41 min Tilt = 40.00 Mean = 106 Exp = 2.225 X/Y/Z = 0.038 0.025 1.458 Errors in X/Y Prediction 0.011 -0.011 um, 0.8 0.9 % Error in Z Prediction 0.079 Predicted X = 0.045 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.101 StdErr = 0.100 nFit = 5 nDrop = 0 Predicted Z = 1.322 StdErr = 0.065 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, -0.115 um; position 0.037, 0.014 Drift reached 4.4 A/sec after 6.2 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.26 nm/sec Tracking image shifted -0.003, -0.011 um; position 0.041, 0.028 Running script # 14 -4.6 1.6 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.5833 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_052_42.0_Oct22_12.45.23.tif Record image shifted 0.001, 0.007 um Tracking reference shifted by 18.8,-3.7 pixels, 0.001,0.007 um Saved Z = 52 at 36.22 min Tilt = 42.00 Mean = 105 Exp = 2.316 X/Y/Z = 0.040 0.023 1.343 Errors in X/Y Prediction -0.004 0.124 um, 0.3 9.5 % Error in Z Prediction 0.020 Predicted X = 0.029 StdErr = 0.000 nFit = 4 nDrop = 0 Predicted Y = -1.711 StdErr = 0.011 nFit = 6 nDrop = 0 Predicted Z = 4.948 StdErr = 0.013 nFit = 4 nDrop = 1 Need regular tracking - Tracking image shifted -0.027, -0.014 um; position 0.057, -1.697 Drift reached 1.7 A/sec after 7.0 sec, going on Running script # 14 4.1 2.2 unbinned pixels; 0.2 1.6 nm along two shift axes; -0.9 1.4 nm on specimen axes Rate = 8.2104 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_053_-64.0_Oct22_12.46.05.tif Record image shifted 0.001, 0.037 um Tracking reference shifted by 103.0,-12.8 pixels, 0.001,0.037 um Saved Z = 53 at 36.93 min Tilt = -64.00 Mean = 102 Exp = 2.995 X/Y/Z = 0.057 -1.736 4.948 Errors in X/Y Prediction 0.028 -0.025 um, 2.1 1.9 % Predicted X = 0.087 StdErr = 0.018 nFit = 4 nDrop = 0 Predicted Y = -1.828 StdErr = 0.016 nFit = 6 nDrop = 0 Predicted Z = 4.950 StdErr = 0.015 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted 0.045, -0.007 um; position 0.042, -1.821 Drift reached 1.5 A/sec after 7.3 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.04 nm/sec Tracking image shifted -0.001, -0.001 um; position 0.045, -1.820 Running script # 14 0.1 1.0 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.4 0.0 nm on specimen axes Rate = 1.82916 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_054_-66.0_Oct22_12.46.57.tif Record image shifted 0.002, 0.031 um Tracking reference shifted by 85.8,-14.3 pixels, 0.002,0.031 um Saved Z = 54 at 37.81 min Tilt = -66.00 Mean = 101 Exp = 3.237 X/Y/Z = 0.043 -1.851 4.915 Errors in X/Y Prediction -0.044 -0.023 um, 3.4 1.8 % Error in Z Prediction -0.035 Predicted X = 0.039 StdErr = 0.043 nFit = 4 nDrop = 0 Predicted Y = -1.958 StdErr = 0.016 nFit = 6 nDrop = 0 Predicted Z = 4.909 StdErr = 0.014 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.084 um; position 0.041, -1.873 Drift reached 1.4 A/sec after 7.8 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.05 nm/sec Tracking image shifted -0.002, -0.002 um; position 0.044, -1.872 Running script # 14 0.5 0.7 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.58173 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_055_-68.0_Oct22_12.47.52.tif Record image shifted 0.001, 0.039 um Tracking reference shifted by 111.1,-12.5 pixels, 0.001,0.039 um Saved Z = 55 at 38.73 min Tilt = -68.00 Mean = 100 Exp = 3.539 X/Y/Z = 0.043 -1.912 4.946 Errors in X/Y Prediction 0.004 0.046 um, 0.3 3.5 % Error in Z Prediction 0.036 Predicted X = 0.044 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.011 StdErr = 0.067 nFit = 5 nDrop = 0 Predicted Z = 1.268 StdErr = 0.026 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.019, 0.060 um; position 0.025, -0.049 Drift reached 2.2 A/sec after 6.4 sec, going on Running script # 14 -2.6 3.5 unbinned pixels; 1.5 0.4 nm along two shift axes; -1.1 -1.0 nm on specimen axes Rate = 7.62189 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_056_44.0_Oct22_12.48.36.tif Record image shifted 0.002, 0.008 um Tracking reference shifted by 21.5,-6.8 pixels, 0.002,0.008 um Saved Z = 56 at 39.44 min Tilt = 44.00 Mean = 105 Exp = 2.423 X/Y/Z = 0.026 -0.055 1.268 Errors in X/Y Prediction -0.019 -0.066 um, 1.4 5.1 % Predicted X = 0.022 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.111 StdErr = 0.067 nFit = 5 nDrop = 0 Predicted Z = 1.184 StdErr = 0.030 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.024 um; position 0.018, -0.088 Drift reached 1.6 A/sec after 6.3 sec, going on Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.11 nm/sec Tracking image shifted -0.004, -0.004 um; position 0.022, -0.084 Running script # 14 -2.1 2.0 unbinned pixels; 0.9 0.5 nm along two shift axes; -0.6 -0.8 nm on specimen axes Rate = 5.05328 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_057_46.0_Oct22_12.49.26.tif Record image shifted 0.000, 0.016 um Tracking reference shifted by 44.1,-4.0 pixels, 0.000,0.016 um Saved Z = 57 at 40.28 min Tilt = 46.00 Mean = 104 Exp = 2.547 X/Y/Z = 0.023 -0.098 1.234 Errors in X/Y Prediction 0.001 0.013 um, 0.0 1.0 % Error in Z Prediction 0.050 Predicted X = 0.012 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.140 StdErr = 0.063 nFit = 5 nDrop = 0 Predicted Z = 1.154 StdErr = 0.036 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.018, 0.020 um; position 0.030, -0.160 Drift reached 1.5 A/sec after 6.5 sec, going on Running script # 14 -4.0 2.2 unbinned pixels; 1.2 1.1 nm along two shift axes; -0.7 -1.5 nm on specimen axes Rate = 7.95788 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_058_48.0_Oct22_12.50.03.tif Record image shifted -0.000, 0.014 um Tracking reference shifted by 40.1,-3.5 pixels, -0.000,0.014 um Saved Z = 58 at 40.90 min Tilt = 48.00 Mean = 103 Exp = 2.692 X/Y/Z = 0.031 -0.172 1.154 Errors in X/Y Prediction 0.019 -0.032 um, 1.5 2.4 % Predicted X = 0.032 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.264 StdErr = 0.043 nFit = 5 nDrop = 0 Predicted Z = 1.082 StdErr = 0.042 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 45 45, reference by 20 20 Tracking image shifted 0.001, -0.084 um; position 0.031, -0.179 Drift reached 2.2 A/sec after 6.9 sec, going on Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.10 nm/sec Tracking image shifted -0.004, -0.004 um; position 0.037, -0.175 Running script # 14 Trimming A by 26 20 20 20, reference by 26 20 20 20 Trimming A by 26 20 20 20, reference by 26 20 20 20 -1.5 1.6 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.5 -0.6 nm on specimen axes Rate = 3.89352 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo2_L1G1_059_50.0_Oct22_12.50.55.tif Record image shifted 0.002, 0.023 um Tracking reference shifted by 65.9,-11.5 pixels, 0.002,0.023 um Saved Z = 59 at 41.77 min Tilt = 50.00 Mean = 103 Exp = 2.870 X/Y/Z = 0.035 -0.198 1.074 2020/10/22 13:11 D:\Users\Ben\201022_Jans_cells\SEM\tomo2_L1G1.st 0 60 3838 3710 1708 -10.0 50.0 2.00 REG 41.8 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Measured defocus = -7.00 microns changed by 3.00 to target drift = 0.03 nm/sec Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.04 nm/sec Need regular tracking - Need autofocus Tracking image shifted 0.000, -0.000 um; position -0.000, 0.000 Drift reached 0.2 A/sec after 5.1 sec, going on Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.02 nm/sec Tracking image shifted 0.000, -0.001 um; position -0.000, 0.001 Running script # 14 -0.5 -0.1 unbinned pixels; 0.0 0.2 nm along two shift axes; 0.1 -0.2 nm on specimen axes Rate = 0.97425 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_000_-10.0_Oct22_13.16.56.tif Record image shifted 0.002, -0.011 um Tracking reference shifted by -30.5,-3.1 pixels, 0.002,-0.011 um Saved Z = 0 at -0.00 min Tilt = -10.00 Mean = 106 Exp = 1.560 X/Y/Z = -0.002 0.012 4.046 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.013 um; position -0.001, 0.025 Drift reached 0.5 A/sec after 5.2 sec, going on Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.03 nm/sec Tracking image shifted -0.000, 0.002 um; position -0.000, 0.023 Running script # 14 0.4 0.0 unbinned pixels; 0.0 0.1 nm along two shift axes; -0.0 0.1 nm on specimen axes Rate = 0.64021 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_001_-12.0_Oct22_13.17.38.tif Record image shifted -0.000, 0.012 um Tracking reference shifted by 34.3,-2.8 pixels, -0.000,0.012 um Saved Z = 1 at 0.70 min Tilt = -12.00 Mean = 110 Exp = 1.613 X/Y/Z = -0.000 0.011 4.129 Predicted X = 0.002 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.010 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 4.211 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.047 um; position -0.003, -0.037 Drift reached 0.7 A/sec after 5.2 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.03 nm/sec Tracking image shifted 0.001, -0.001 um; position -0.004, -0.036 Running script # 14 -0.5 -0.0 unbinned pixels; 0.0 0.2 nm along two shift axes; 0.0 -0.2 nm on specimen axes Rate = 0.89935 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_002_-14.0_Oct22_13.18.20.tif Record image shifted -0.000, 0.011 um Tracking reference shifted by 31.0,-2.7 pixels, -0.000,0.011 um Saved Z = 2 at 1.40 min Tilt = -14.00 Mean = 109 Exp = 1.616 X/Y/Z = -0.004 -0.047 4.201 Errors in X/Y Prediction -0.005 -0.057 um, 0.4 4.3 % Error in Z Prediction -0.009 Need regular tracking - Need autofocus Tracking image shifted 0.012, 0.205 um; position -0.014, -0.194 Drift reached 2.9 A/sec after 5.1 sec, going on Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.13 nm/sec Tracking image shifted 0.004, 0.006 um; position -0.018, -0.201 Running script # 14 1.7 0.1 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.1 0.6 nm on specimen axes Rate = 2.9487 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_003_-8.0_Oct22_13.19.06.tif Record image shifted 0.000, -0.010 um Tracking reference shifted by -29.2,2.2 pixels, 0.000,-0.010 um Saved Z = 3 at 2.25 min Tilt = -8.00 Mean = 110 Exp = 1.613 X/Y/Z = -0.018 -0.191 3.964 Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.046 um; position -0.024, -0.238 Drift reached 1.1 A/sec after 5.1 sec, going on Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.07 nm/sec Tracking image shifted 0.001, -0.003 um; position -0.025, -0.234 Running script # 14 -1.0 0.4 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.1 -0.4 nm on specimen axes Rate = 1.88869 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_004_-6.0_Oct22_13.19.55.tif Record image shifted -0.000, -0.014 um Tracking reference shifted by -39.0,4.1 pixels, -0.000,-0.014 um Saved Z = 4 at 2.99 min Tilt = -6.00 Mean = 110 Exp = 1.611 X/Y/Z = -0.024 -0.220 3.896 Predicted X = -0.003 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.066 StdErr = 0.042 nFit = 3 nDrop = 0 Predicted Z = 4.281 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.010, 0.107 um; position -0.013, -0.173 Drift reached 1.5 A/sec after 5.1 sec, going on Running script # 14 -2.1 1.0 unbinned pixels; 0.6 0.6 nm along two shift axes; -0.3 -0.8 nm on specimen axes Rate = 4.00939 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_005_-16.0_Oct22_13.20.27.tif Record image shifted 0.002, 0.009 um Tracking reference shifted by 24.4,-8.8 pixels, 0.002,0.009 um Saved Z = 5 at 3.52 min Tilt = -16.00 Mean = 110 Exp = 1.627 X/Y/Z = -0.015 -0.181 4.281 Errors in X/Y Prediction -0.011 -0.114 um, 0.9 8.7 % Predicted X = -0.020 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.263 StdErr = 0.057 nFit = 3 nDrop = 0 Predicted Z = 4.358 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, -0.062 um; position -0.020, -0.202 Drift reached 1.4 A/sec after 5.1 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.002 um; position -0.019, -0.205 Running script # 14 0.6 0.3 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 1.22194 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_006_-18.0_Oct22_13.21.09.tif Record image shifted -0.002, 0.016 um Tracking reference shifted by 45.6,1.3 pixels, -0.002,0.016 um Saved Z = 6 at 4.23 min Tilt = -18.00 Mean = 109 Exp = 1.631 X/Y/Z = -0.017 -0.221 4.362 Errors in X/Y Prediction 0.003 0.043 um, 0.2 3.3 % Error in Z Prediction 0.003 Predicted X = -0.031 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.248 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.827 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, -0.078 um; position -0.026, -0.170 Drift reached 0.7 A/sec after 10.2 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.04 nm/sec Tracking image shifted -0.001, 0.003 um; position -0.024, -0.174 Running script # 14 0.6 0.4 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.27303 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_007_-4.0_Oct22_13.22.02.tif Record image shifted -0.000, -0.011 um Tracking reference shifted by -30.4,2.7 pixels, -0.000,-0.011 um Saved Z = 7 at 5.11 min Tilt = -4.00 Mean = 110 Exp = 1.608 X/Y/Z = -0.024 -0.163 3.850 Errors in X/Y Prediction 0.006 0.085 um, 0.5 6.5 % Error in Z Prediction 0.022 Predicted X = -0.029 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.163 StdErr = 0.063 nFit = 3 nDrop = 0 Predicted Z = 3.789 StdErr = 0.017 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, 0.005 um; position -0.032, -0.168 Drift reached 1.3 A/sec after 5.2 sec, going on Running script # 14 -2.4 0.9 unbinned pixels; 0.6 0.7 nm along two shift axes; -0.3 -0.9 nm on specimen axes Rate = 4.46064 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_008_-2.0_Oct22_13.22.36.tif Record image shifted -0.000, -0.016 um Tracking reference shifted by -44.3,4.3 pixels, -0.000,-0.016 um Saved Z = 8 at 5.66 min Tilt = -2.00 Mean = 110 Exp = 1.606 X/Y/Z = -0.032 -0.151 3.789 Errors in X/Y Prediction -0.003 0.012 um, 0.3 1.0 % Predicted X = -0.025 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.323 StdErr = 0.070 nFit = 3 nDrop = 0 Predicted Z = 4.439 StdErr = 0.006 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.008, -0.035 um; position -0.018, -0.289 Drift reached 0.5 A/sec after 5.4 sec, going on Running script # 14 -1.1 0.6 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.2 -0.4 nm on specimen axes Rate = 2.10924 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_009_-20.0_Oct22_13.23.09.tif Record image shifted -0.001, 0.008 um Tracking reference shifted by 22.7,0.7 pixels, -0.001,0.008 um Saved Z = 9 at 6.21 min Tilt = -20.00 Mean = 109 Exp = 1.642 X/Y/Z = -0.016 -0.296 4.439 Errors in X/Y Prediction 0.009 0.027 um, 0.7 2.1 % Predicted X = -0.008 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Y = -0.310 StdErr = 0.085 nFit = 4 nDrop = 0 Predicted Z = 4.517 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.010, 0.054 um; position -0.019, -0.364 Drift reached 1.4 A/sec after 5.3 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.07 nm/sec Tracking image shifted 0.001, 0.003 um; position -0.019, -0.367 Running script # 14 0.9 0.6 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.90761 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_010_-22.0_Oct22_13.23.52.tif Record image shifted 0.001, 0.016 um Tracking reference shifted by 43.8,-6.2 pixels, 0.001,0.016 um Saved Z = 10 at 6.95 min Tilt = -22.00 Mean = 109 Exp = 1.657 X/Y/Z = -0.019 -0.383 4.483 Errors in X/Y Prediction -0.011 -0.073 um, 0.8 5.6 % Error in Z Prediction -0.034 Predicted X = -0.034 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.109 StdErr = 0.033 nFit = 3 nDrop = 0 Predicted Z = 3.732 StdErr = 0.022 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.010, -0.002 um; position -0.025, -0.106 Drift reached 2.2 A/sec after 5.1 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.10 nm/sec Tracking image shifted -0.001, 0.005 um; position -0.024, -0.113 Running script # 14 1.0 0.7 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.09052 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_011_0.0_Oct22_13.24.39.tif Record image shifted -0.000, -0.009 um Tracking reference shifted by -24.2,2.6 pixels, -0.000,-0.009 um Saved Z = 11 at 7.73 min Tilt = 0.00 Mean = 110 Exp = 1.608 X/Y/Z = -0.023 -0.104 3.734 Errors in X/Y Prediction 0.011 0.004 um, 0.9 0.3 % Error in Z Prediction 0.002 Predicted X = -0.025 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.081 StdErr = 0.025 nFit = 3 nDrop = 0 Predicted Z = 3.682 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, 0.072 um; position -0.030, -0.153 Drift reached 1.5 A/sec after 5.2 sec, going on Running script # 14 -2.4 1.7 unbinned pixels; 0.8 0.6 nm along two shift axes; -0.5 -0.9 nm on specimen axes Rate = 5.14586 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_012_2.0_Oct22_13.25.13.tif Record image shifted 0.001, -0.018 um Tracking reference shifted by -50.7,0.4 pixels, 0.001,-0.018 um Saved Z = 12 at 8.29 min Tilt = 2.00 Mean = 109 Exp = 1.608 X/Y/Z = -0.030 -0.134 3.682 Errors in X/Y Prediction -0.005 -0.053 um, 0.4 4.1 % Predicted X = -0.021 StdErr = 0.005 nFit = 4 nDrop = 0 Predicted Y = -0.433 StdErr = 0.063 nFit = 5 nDrop = 0 Predicted Z = 4.560 StdErr = 0.025 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.008, 0.059 um; position -0.013, -0.491 Drift reached 0.5 A/sec after 5.4 sec, going on Running script # 14 -0.5 0.8 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.2 -0.2 nm on specimen axes Rate = 1.56934 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_013_-24.0_Oct22_13.25.47.tif Record image shifted 0.001, 0.009 um Tracking reference shifted by 26.1,-4.6 pixels, 0.001,0.009 um Saved Z = 13 at 8.86 min Tilt = -24.00 Mean = 109 Exp = 1.670 X/Y/Z = -0.014 -0.501 4.560 Errors in X/Y Prediction 0.007 -0.068 um, 0.6 5.2 % Predicted X = -0.014 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.640 StdErr = 0.009 nFit = 5 nDrop = 0 Predicted Z = 4.630 StdErr = 0.029 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.111 um; position -0.018, -0.529 Drift reached 1.2 A/sec after 5.3 sec, going on Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.06 nm/sec Tracking image shifted -0.001, 0.003 um; position -0.016, -0.532 Running script # 14 0.6 0.7 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.60118 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_014_-26.0_Oct22_13.26.30.tif Record image shifted -0.000, 0.015 um Tracking reference shifted by 41.5,-2.3 pixels, -0.000,0.015 um Saved Z = 14 at 9.57 min Tilt = -26.00 Mean = 109 Exp = 1.689 X/Y/Z = -0.016 -0.547 4.555 Errors in X/Y Prediction -0.002 0.093 um, 0.2 7.1 % Error in Z Prediction -0.075 Predicted X = -0.028 StdErr = 0.021 nFit = 4 nDrop = 0 Predicted Y = -0.157 StdErr = 0.072 nFit = 4 nDrop = 0 Predicted Z = 3.627 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.070 um; position -0.029, -0.088 Drift reached 2.3 A/sec after 5.2 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.09 nm/sec Tracking image shifted -0.002, 0.004 um; position -0.027, -0.093 Running script # 14 0.5 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.61259 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_015_4.0_Oct22_13.27.17.tif Record image shifted -0.002, -0.007 um Tracking reference shifted by -20.6,7.0 pixels, -0.002,-0.007 um Saved Z = 15 at 10.36 min Tilt = 4.00 Mean = 110 Exp = 1.616 X/Y/Z = -0.025 -0.085 3.655 Errors in X/Y Prediction 0.004 0.072 um, 0.3 5.5 % Error in Z Prediction 0.028 Predicted X = -0.028 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.077 StdErr = 0.058 nFit = 5 nDrop = 0 Predicted Z = 3.601 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.044 um; position -0.032, -0.121 Drift reached 3.8 A/sec after 5.1 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.27 nm/sec Tracking image shifted -0.000, -0.010 um; position -0.031, -0.110 Running script # 14 -4.8 1.2 unbinned pixels; 1.0 1.4 nm along two shift axes; -0.3 -1.7 nm on specimen axes Rate = 8.66097 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_016_6.0_Oct22_13.28.03.tif Record image shifted -0.001, -0.016 um Tracking reference shifted by -44.2,5.5 pixels, -0.001,-0.016 um Saved Z = 16 at 11.12 min Tilt = 6.00 Mean = 109 Exp = 1.617 X/Y/Z = -0.030 -0.092 3.604 Errors in X/Y Prediction -0.003 -0.015 um, 0.2 1.1 % Error in Z Prediction 0.003 Predicted X = -0.012 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.633 StdErr = 0.050 nFit = 5 nDrop = 0 Predicted Z = 4.611 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.012 um; position -0.014, -0.621 Drift reached 0.2 A/sec after 5.4 sec, going on Running script # 14 -0.0 0.7 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 -0.0 nm on specimen axes Rate = 1.18104 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_017_-28.0_Oct22_13.28.37.tif Record image shifted -0.000, 0.011 um Tracking reference shifted by 31.4,-1.4 pixels, -0.000,0.011 um Saved Z = 17 at 11.70 min Tilt = -28.00 Mean = 109 Exp = 1.707 X/Y/Z = -0.013 -0.632 4.611 Errors in X/Y Prediction -0.000 0.001 um, 0.0 0.1 % Predicted X = -0.013 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.685 StdErr = 0.042 nFit = 5 nDrop = 0 Predicted Z = 4.659 StdErr = 0.037 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.002 um; position -0.014, -0.684 Drift reached 0.7 A/sec after 5.4 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.05 nm/sec Tracking image shifted -0.000, 0.002 um; position -0.013, -0.686 Running script # 14 0.6 0.6 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.46551 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_018_-30.0_Oct22_13.29.21.tif Record image shifted 0.001, 0.013 um Tracking reference shifted by 37.2,-5.3 pixels, 0.001,0.013 um Saved Z = 18 at 12.42 min Tilt = -30.00 Mean = 108 Exp = 1.728 X/Y/Z = -0.014 -0.700 4.599 Errors in X/Y Prediction -0.001 -0.014 um, 0.0 1.1 % Error in Z Prediction -0.060 Predicted X = -0.030 StdErr = 0.015 nFit = 4 nDrop = 0 Predicted Y = -0.084 StdErr = 0.041 nFit = 6 nDrop = 0 Predicted Z = 3.564 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, -0.011 um; position -0.024, -0.073 Drift reached 0.4 A/sec after 5.1 sec, going on Running script # 14 -0.2 1.1 unbinned pixels; 0.4 0.1 nm along two shift axes; -0.4 -0.1 nm on specimen axes Rate = 1.95461 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_019_8.0_Oct22_13.29.57.tif Record image shifted -0.001, -0.008 um Tracking reference shifted by -22.6,4.1 pixels, -0.001,-0.008 um Saved Z = 19 at 13.02 min Tilt = 8.00 Mean = 109 Exp = 1.627 X/Y/Z = -0.023 -0.065 3.564 Errors in X/Y Prediction 0.007 0.019 um, 0.5 1.5 % Predicted X = -0.024 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.055 StdErr = 0.043 nFit = 6 nDrop = 0 Predicted Z = 3.522 StdErr = 0.012 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, 0.038 um; position -0.033, -0.093 Drift reached 4.5 A/sec after 5.4 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.32 nm/sec Tracking image shifted -0.001, -0.012 um; position -0.030, -0.078 Running script # 14 -5.6 1.5 unbinned pixels; 1.1 1.7 nm along two shift axes; -0.3 -2.0 nm on specimen axes Rate = 10.19414 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_020_10.0_Oct22_13.30.43.tif Record image shifted 0.000, -0.018 um Tracking reference shifted by -50.7,4.3 pixels, 0.000,-0.018 um Saved Z = 20 at 13.87 min Tilt = 10.00 Mean = 109 Exp = 1.638 X/Y/Z = -0.030 -0.058 3.508 Errors in X/Y Prediction -0.006 -0.003 um, 0.5 0.3 % Error in Z Prediction -0.013 Predicted X = -0.012 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.751 StdErr = 0.043 nFit = 5 nDrop = 0 Predicted Z = 4.633 StdErr = 0.017 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, 0.006 um; position -0.010, -0.757 Drift reached 0.5 A/sec after 5.4 sec, going on Running script # 14 0.4 0.6 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.3219 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_021_-32.0_Oct22_13.31.23.tif Record image shifted -0.001, 0.012 um Tracking reference shifted by 35.0,-0.4 pixels, -0.001,0.012 um Saved Z = 21 at 14.46 min Tilt = -32.00 Mean = 108 Exp = 1.754 X/Y/Z = -0.009 -0.769 4.633 Errors in X/Y Prediction 0.003 -0.018 um, 0.2 1.4 % Predicted X = -0.008 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.851 StdErr = 0.024 nFit = 5 nDrop = 0 Predicted Z = 4.662 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.040 um; position -0.011, -0.810 Drift reached 1.5 A/sec after 5.5 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.06 nm/sec Tracking image shifted -0.001, 0.002 um; position -0.010, -0.813 Running script # 14 0.4 0.5 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.16658 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_022_-34.0_Oct22_13.32.07.tif Record image shifted 0.001, 0.013 um Tracking reference shifted by 36.1,-6.3 pixels, 0.001,0.013 um Saved Z = 22 at 15.18 min Tilt = -34.00 Mean = 108 Exp = 1.780 X/Y/Z = -0.011 -0.826 4.644 Errors in X/Y Prediction -0.002 0.025 um, 0.2 1.9 % Error in Z Prediction -0.018 Predicted X = -0.027 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -0.031 StdErr = 0.038 nFit = 6 nDrop = 0 Predicted Z = 3.466 StdErr = 0.010 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.004 um; position -0.028, -0.027 Drift reached 6.1 A/sec after 5.4 sec, going on Running script # 14 -8.9 2.9 unbinned pixels; 2.0 2.6 nm along two shift axes; -0.7 -3.2 nm on specimen axes Rate = 16.35353 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_023_12.0_Oct22_13.32.44.tif Record image shifted -0.001, -0.020 um Tracking reference shifted by -57.6,8.2 pixels, -0.001,-0.020 um Saved Z = 23 at 15.80 min Tilt = 12.00 Mean = 109 Exp = 1.649 X/Y/Z = -0.026 -0.000 3.466 Errors in X/Y Prediction 0.002 0.031 um, 0.1 2.4 % Predicted X = -0.029 StdErr = 0.018 nFit = 4 nDrop = 0 Predicted Y = 0.056 StdErr = 0.027 nFit = 5 nDrop = 0 Predicted Z = 3.421 StdErr = 0.011 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, 0.101 um; position -0.023, -0.045 Drift reached 3.9 A/sec after 5.3 sec, going on Measured defocus = -3.90 microns changed by -0.10 to target drift = 0.13 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.08 nm/sec Tracking image shifted -0.003, 0.004 um; position -0.018, -0.050 Running script # 14 -1.2 3.3 unbinned pixels; 1.2 0.0 nm along two shift axes; -1.1 -0.5 nm on specimen axes Rate = 6.13036 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_024_14.0_Oct22_13.33.42.tif Record image shifted 0.002, -0.003 um Tracking reference shifted by -8.1,-4.4 pixels, 0.002,-0.003 um Saved Z = 24 at 16.77 min Tilt = 14.00 Mean = 109 Exp = 1.662 X/Y/Z = -0.019 -0.047 3.320 Errors in X/Y Prediction 0.011 -0.103 um, 0.8 7.9 % Error in Z Prediction -0.101 Predicted X = -0.009 StdErr = 0.008 nFit = 4 nDrop = 0 Predicted Y = -0.876 StdErr = 0.007 nFit = 5 nDrop = 0 Predicted Z = 4.667 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.040 um; position -0.009, -0.916 Drift reached 0.9 A/sec after 5.6 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.04 nm/sec Tracking image shifted -0.001, -0.000 um; position -0.008, -0.916 Running script # 14 -0.0 0.6 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 -0.0 nm on specimen axes Rate = 1.05392 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_025_-36.0_Oct22_13.34.32.tif Record image shifted -0.000, 0.012 um Tracking reference shifted by 33.7,-2.9 pixels, -0.000,0.012 um Saved Z = 25 at 17.60 min Tilt = -36.00 Mean = 108 Exp = 1.817 X/Y/Z = -0.008 -0.928 4.637 Errors in X/Y Prediction 0.001 -0.052 um, 0.1 4.0 % Error in Z Prediction -0.030 Predicted X = -0.009 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -1.014 StdErr = 0.027 nFit = 5 nDrop = 0 Predicted Z = 4.667 StdErr = 0.019 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, -0.057 um; position -0.010, -0.958 Drift reached 1.4 A/sec after 5.6 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.002 um; position -0.009, -0.961 Running script # 14 0.5 0.4 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.18475 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_026_-38.0_Oct22_13.35.16.tif Record image shifted 0.000, 0.014 um Tracking reference shifted by 38.2,-3.9 pixels, 0.000,0.014 um Saved Z = 26 at 18.34 min Tilt = -38.00 Mean = 108 Exp = 1.852 X/Y/Z = -0.009 -0.974 4.651 Errors in X/Y Prediction -0.001 0.040 um, 0.0 3.1 % Error in Z Prediction -0.016 Predicted X = -0.014 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.054 StdErr = 0.064 nFit = 5 nDrop = 0 Predicted Z = 3.276 StdErr = 0.046 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, -0.083 um; position -0.022, 0.030 Drift reached 6.2 A/sec after 5.4 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.28 nm/sec Tracking image shifted -0.005, -0.017 um; position -0.016, 0.049 Running script # 14 -4.0 1.2 unbinned pixels; 0.9 1.2 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 7.30039 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_027_16.0_Oct22_13.36.06.tif Record image shifted 0.000, -0.015 um Tracking reference shifted by -41.1,3.3 pixels, 0.000,-0.015 um Saved Z = 27 at 19.17 min Tilt = 16.00 Mean = 109 Exp = 1.674 X/Y/Z = -0.016 0.065 3.213 Errors in X/Y Prediction -0.002 0.119 um, 0.2 9.1 % Error in Z Prediction -0.063 Predicted X = -0.010 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.093 StdErr = 0.075 nFit = 6 nDrop = 0 Predicted Z = 3.155 StdErr = 0.050 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.012, 0.114 um; position -0.022, -0.021 Drift reached 3.2 A/sec after 5.4 sec, going on Running script # 14 -2.7 8.3 unbinned pixels; 3.1 0.1 nm along two shift axes; -2.8 -1.2 nm on specimen axes Rate = 15.42723 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_028_18.0_Oct22_13.36.45.tif Record image shifted -0.001, -0.008 um Tracking reference shifted by -22.9,4.7 pixels, -0.001,-0.008 um Saved Z = 28 at 19.82 min Tilt = 18.00 Mean = 109 Exp = 1.694 X/Y/Z = -0.016 -0.012 3.155 Errors in X/Y Prediction -0.006 -0.105 um, 0.5 8.0 % Predicted X = -0.008 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -1.051 StdErr = 0.039 nFit = 5 nDrop = 0 Predicted Z = 4.666 StdErr = 0.017 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, 0.003 um; position -0.010, -1.054 Drift reached 0.5 A/sec after 5.6 sec, going on Running script # 14 0.6 0.7 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.64344 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_029_-40.0_Oct22_13.37.22.tif Record image shifted -0.001, 0.014 um Tracking reference shifted by 38.9,-0.7 pixels, -0.001,0.014 um Saved Z = 29 at 20.44 min Tilt = -40.00 Mean = 107 Exp = 1.893 X/Y/Z = -0.009 -1.069 4.666 Errors in X/Y Prediction -0.001 -0.017 um, 0.1 1.3 % Predicted X = -0.009 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -1.147 StdErr = 0.041 nFit = 5 nDrop = 0 Predicted Z = 4.679 StdErr = 0.020 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.024 um; position -0.012, -1.123 Drift reached 1.7 A/sec after 5.6 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.07 nm/sec Tracking image shifted -0.000, 0.002 um; position -0.011, -1.127 Running script # 14 0.8 0.4 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.54271 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_030_-42.0_Oct22_13.38.07.tif Record image shifted 0.001, 0.013 um Tracking reference shifted by 36.2,-6.6 pixels, 0.001,0.013 um Saved Z = 30 at 21.19 min Tilt = -42.00 Mean = 107 Exp = 1.938 X/Y/Z = -0.012 -1.140 4.671 Errors in X/Y Prediction -0.003 0.007 um, 0.2 0.5 % Error in Z Prediction -0.008 Predicted X = -0.013 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -1.211 StdErr = 0.039 nFit = 5 nDrop = 0 Predicted Z = 4.676 StdErr = 0.012 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, 0.019 um; position -0.017, -1.230 Drift reached 0.3 A/sec after 5.8 sec, going on Running script # 14 -0.2 0.7 unbinned pixels; 0.3 0.0 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.26419 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_031_-44.0_Oct22_13.38.40.tif Record image shifted -0.000, 0.013 um Tracking reference shifted by 36.4,-2.6 pixels, -0.000,0.013 um Saved Z = 31 at 21.75 min Tilt = -44.00 Mean = 107 Exp = 1.987 X/Y/Z = -0.017 -1.243 4.676 Errors in X/Y Prediction -0.004 -0.032 um, 0.3 2.4 % Predicted X = -0.014 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.005 StdErr = 0.095 nFit = 6 nDrop = 0 Predicted Z = 3.076 StdErr = 0.056 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, -0.053 um; position -0.023, 0.058 Drift reached 4.5 A/sec after 5.4 sec, going on Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.29 nm/sec Tracking image shifted -0.004, -0.011 um; position -0.018, 0.071 Running script # 14 -4.8 1.8 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.5 -1.7 nm on specimen axes Rate = 8.98356 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_032_20.0_Oct22_13.39.31.tif Record image shifted 0.001, -0.019 um Tracking reference shifted by -54.9,2.7 pixels, 0.001,-0.019 um Saved Z = 32 at 22.59 min Tilt = 20.00 Mean = 109 Exp = 1.714 X/Y/Z = -0.018 0.093 2.999 Errors in X/Y Prediction -0.004 0.087 um, 0.3 6.7 % Error in Z Prediction -0.077 Predicted X = -0.019 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.142 StdErr = 0.145 nFit = 5 nDrop = 0 Predicted Z = 2.903 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.134 um; position -0.019, 0.008 Drift reached 3.5 A/sec after 5.3 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.18 nm/sec Tracking image shifted -0.003, -0.005 um; position -0.014, 0.014 Running script # 14 -1.3 2.1 unbinned pixels; 0.9 0.2 nm along two shift axes; -0.7 -0.5 nm on specimen axes Rate = 4.37284 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_033_22.0_Oct22_13.40.17.tif Record image shifted -0.000, -0.011 um Tracking reference shifted by -31.2,4.1 pixels, -0.000,-0.011 um Saved Z = 33 at 23.35 min Tilt = 22.00 Mean = 109 Exp = 1.738 X/Y/Z = -0.013 0.026 2.933 Errors in X/Y Prediction 0.006 -0.117 um, 0.4 8.9 % Error in Z Prediction 0.030 Predicted X = -0.008 StdErr = 0.005 nFit = 4 nDrop = 0 Predicted Y = -0.011 StdErr = 0.145 nFit = 5 nDrop = 0 Predicted Z = 2.820 StdErr = 0.025 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, -0.037 um; position -0.015, 0.026 Drift reached 3.8 A/sec after 5.4 sec, going on Running script # 14 -6.9 1.9 unbinned pixels; 1.4 2.1 nm along two shift axes; -0.5 -2.5 nm on specimen axes Rate = 12.62944 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_034_24.0_Oct22_13.40.50.tif Record image shifted -0.002, -0.022 um Tracking reference shifted by -61.6,10.5 pixels, -0.002,-0.022 um Saved Z = 34 at 23.91 min Tilt = 24.00 Mean = 108 Exp = 1.758 X/Y/Z = -0.012 0.052 2.820 Errors in X/Y Prediction -0.004 0.063 um, 0.3 4.9 % Predicted X = -0.024 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = -1.329 StdErr = 0.032 nFit = 6 nDrop = 0 Predicted Z = 4.683 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.014, 0.032 um; position -0.011, -1.361 Drift reached 2.9 A/sec after 5.8 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.12 nm/sec Tracking image shifted -0.001, 0.005 um; position -0.009, -1.367 Running script # 14 1.4 0.7 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 2.78921 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_035_-46.0_Oct22_13.41.41.tif Record image shifted -0.000, 0.015 um Tracking reference shifted by 42.8,-3.6 pixels, -0.000,0.015 um Saved Z = 35 at 24.76 min Tilt = -46.00 Mean = 107 Exp = 2.044 X/Y/Z = -0.009 -1.383 4.648 Errors in X/Y Prediction 0.016 -0.054 um, 1.2 4.1 % Error in Z Prediction -0.035 Predicted X = 0.001 StdErr = 0.009 nFit = 4 nDrop = 0 Predicted Y = -1.520 StdErr = 0.033 nFit = 5 nDrop = 0 Predicted Z = 4.661 StdErr = 0.018 nFit = 5 nDrop = 0 Need regular tracking - Tracking image shifted 0.016, -0.075 um; position -0.015, -1.445 Drift reached 1.9 A/sec after 5.8 sec, going on Running script # 14 1.7 1.9 unbinned pixels; 0.4 0.8 nm along two shift axes; -0.7 0.5 nm on specimen axes Rate = 4.47045 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_036_-48.0_Oct22_13.42.14.tif Record image shifted -0.001, 0.013 um Tracking reference shifted by 37.9,-1.1 pixels, -0.001,0.013 um Saved Z = 36 at 25.31 min Tilt = -48.00 Mean = 106 Exp = 2.103 X/Y/Z = -0.013 -1.460 4.661 Errors in X/Y Prediction -0.014 0.060 um, 1.1 4.6 % Predicted X = -0.011 StdErr = 0.016 nFit = 4 nDrop = 0 Predicted Y = -1.591 StdErr = 0.038 nFit = 6 nDrop = 0 Predicted Z = 4.664 StdErr = 0.020 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.060 um; position -0.015, -1.531 Drift reached 1.7 A/sec after 6.0 sec, going on Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.06 nm/sec Tracking image shifted -0.001, 0.002 um; position -0.014, -1.534 Running script # 14 0.8 0.8 unbinned pixels; 0.2 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.00781 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_037_-50.0_Oct22_13.43.00.tif Record image shifted 0.002, 0.016 um Tracking reference shifted by 44.3,-8.5 pixels, 0.002,0.016 um Saved Z = 37 at 26.08 min Tilt = -50.00 Mean = 106 Exp = 2.178 X/Y/Z = -0.015 -1.550 4.589 Errors in X/Y Prediction -0.004 0.041 um, 0.3 3.1 % Error in Z Prediction -0.075 Predicted X = -0.025 StdErr = 0.009 nFit = 4 nDrop = 0 Predicted Y = -1.656 StdErr = 0.043 nFit = 6 nDrop = 0 Predicted Z = 4.598 StdErr = 0.040 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.007, -0.007 um; position -0.018, -1.650 Drift reached 0.6 A/sec after 6.2 sec, going on Running script # 14 0.9 1.7 unbinned pixels; 0.4 0.5 nm along two shift axes; -0.6 0.3 nm on specimen axes Rate = 3.34359 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_038_-52.0_Oct22_13.43.34.tif Record image shifted -0.001, 0.016 um Tracking reference shifted by 45.0,-0.3 pixels, -0.001,0.016 um Saved Z = 38 at 26.65 min Tilt = -52.00 Mean = 106 Exp = 2.266 X/Y/Z = -0.016 -1.666 4.598 Errors in X/Y Prediction 0.009 -0.010 um, 0.7 0.7 % Predicted X = -0.008 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.066 StdErr = 0.132 nFit = 5 nDrop = 0 Predicted Z = 2.721 StdErr = 0.029 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, -0.012 um; position -0.001, 0.078 Drift reached 4.9 A/sec after 5.3 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.33 nm/sec Tracking image shifted -0.002, -0.012 um; position 0.001, 0.092 Running script # 14 -5.3 1.8 unbinned pixels; 1.2 1.6 nm along two shift axes; -0.5 -1.9 nm on specimen axes Rate = 9.836 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_039_26.0_Oct22_13.44.25.tif Record image shifted 0.002, -0.009 um Tracking reference shifted by -24.7,-4.0 pixels, 0.002,-0.009 um Saved Z = 39 at 27.50 min Tilt = 26.00 Mean = 108 Exp = 1.786 X/Y/Z = -0.000 0.103 2.675 Errors in X/Y Prediction 0.008 0.037 um, 0.6 2.8 % Error in Z Prediction -0.046 Predicted X = 0.005 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = 0.102 StdErr = 0.123 nFit = 5 nDrop = 0 Predicted Z = 2.584 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.011, 0.131 um; position -0.007, -0.029 Drift reached 3.0 A/sec after 5.5 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.14 nm/sec Tracking image shifted -0.003, -0.004 um; position -0.002, -0.024 Running script # 14 -0.7 2.3 unbinned pixels; 0.8 0.0 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.21988 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_040_28.0_Oct22_13.45.13.tif Record image shifted -0.001, -0.011 um Tracking reference shifted by -31.8,4.8 pixels, -0.001,-0.011 um Saved Z = 40 at 28.28 min Tilt = 28.00 Mean = 108 Exp = 1.819 X/Y/Z = -0.001 -0.013 2.597 Errors in X/Y Prediction -0.006 -0.115 um, 0.4 8.8 % Error in Z Prediction 0.013 Predicted X = 0.007 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.025 StdErr = 0.140 nFit = 5 nDrop = 0 Predicted Z = 2.482 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.011, -0.016 um; position -0.004, -0.008 Drift reached 4.5 A/sec after 5.6 sec, going on Running script # 14 -8.8 2.4 unbinned pixels; 1.8 2.6 nm along two shift axes; -0.6 -3.2 nm on specimen axes Rate = 16.02443 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_041_30.0_Oct22_13.45.47.tif Record image shifted -0.000, -0.023 um Tracking reference shifted by -65.1,5.9 pixels, -0.000,-0.023 um Saved Z = 41 at 28.86 min Tilt = 30.00 Mean = 107 Exp = 1.856 X/Y/Z = -0.003 0.021 2.482 Errors in X/Y Prediction -0.010 0.045 um, 0.7 3.5 % Predicted X = -0.004 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.061 StdErr = 0.121 nFit = 5 nDrop = 0 Predicted Z = 2.376 StdErr = 0.044 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.001 um; position -0.005, -0.062 Drift reached 2.1 A/sec after 5.6 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.15 nm/sec Tracking image shifted -0.005, -0.004 um; position 0.002, -0.058 Running script # 14 -1.0 2.4 unbinned pixels; 0.9 0.1 nm along two shift axes; -0.8 -0.4 nm on specimen axes Rate = 4.5833 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_042_32.0_Oct22_13.46.34.tif Record image shifted 0.001, -0.008 um Tracking reference shifted by -23.0,0.3 pixels, 0.001,-0.008 um Saved Z = 42 at 29.65 min Tilt = 32.00 Mean = 108 Exp = 1.900 X/Y/Z = 0.002 -0.049 2.351 Errors in X/Y Prediction 0.006 0.012 um, 0.5 0.9 % Error in Z Prediction -0.025 Predicted X = -0.018 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -1.747 StdErr = 0.048 nFit = 5 nDrop = 0 Predicted Z = 4.587 StdErr = 0.045 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.025, 0.035 um; position 0.008, -1.782 Drift reached 2.9 A/sec after 6.2 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.13 nm/sec Tracking image shifted -0.002, 0.005 um; position 0.010, -1.789 Running script # 14 1.5 1.2 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.5 0.5 nm on specimen axes Rate = 3.39753 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_043_-54.0_Oct22_13.47.28.tif Record image shifted 0.001, 0.016 um Tracking reference shifted by 45.1,-8.0 pixels, 0.001,0.016 um Saved Z = 43 at 30.63 min Tilt = -54.00 Mean = 105 Exp = 2.362 X/Y/Z = 0.009 -1.805 4.544 Errors in X/Y Prediction 0.026 -0.059 um, 2.0 4.5 % Error in Z Prediction -0.043 Predicted X = 0.016 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = -1.963 StdErr = 0.007 nFit = 5 nDrop = 1 Predicted Z = 4.515 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.017, -0.074 um; position -0.001, -1.889 Drift reached 1.9 A/sec after 6.4 sec, going on Running script # 14 1.0 1.4 unbinned pixels; 0.4 0.5 nm along two shift axes; -0.5 0.3 nm on specimen axes Rate = 3.15482 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_044_-56.0_Oct22_13.48.08.tif Record image shifted -0.001, 0.016 um Tracking reference shifted by 44.4,-1.0 pixels, -0.001,0.016 um Saved Z = 44 at 31.21 min Tilt = -56.00 Mean = 104 Exp = 2.486 X/Y/Z = 0.002 -1.906 4.515 Errors in X/Y Prediction -0.015 0.057 um, 1.1 4.4 % Predicted X = 0.015 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Y = -2.044 StdErr = 0.037 nFit = 5 nDrop = 0 Predicted Z = 4.489 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.012, -0.099 um; position 0.003, -1.945 Drift reached 1.8 A/sec after 6.6 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.06 nm/sec Tracking image shifted -0.001, 0.001 um; position 0.006, -1.947 Running script # 14 0.6 0.9 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.95703 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_045_-58.0_Oct22_13.48.57.tif Record image shifted 0.002, 0.017 um Tracking reference shifted by 48.6,-8.6 pixels, 0.002,0.017 um Saved Z = 45 at 32.03 min Tilt = -58.00 Mean = 104 Exp = 2.617 X/Y/Z = 0.004 -1.965 4.437 Errors in X/Y Prediction -0.011 0.079 um, 0.8 6.1 % Error in Z Prediction -0.052 Predicted X = 0.001 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -2.023 StdErr = 0.036 nFit = 5 nDrop = 0 Predicted Z = 4.409 StdErr = 0.040 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, 0.046 um; position 0.002, -2.068 Drift reached 1.0 A/sec after 6.7 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.06 nm/sec Tracking image shifted -0.001, 0.000 um; position 0.004, -2.069 Running script # 14 0.2 0.9 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.3 0.0 nm on specimen axes Rate = 1.55227 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_046_-60.0_Oct22_13.49.48.tif Record image shifted 0.000, 0.018 um Tracking reference shifted by 51.9,-5.7 pixels, 0.000,0.018 um Saved Z = 46 at 32.89 min Tilt = -60.00 Mean = 102 Exp = 2.773 X/Y/Z = 0.004 -2.087 4.359 Errors in X/Y Prediction 0.003 -0.065 um, 0.3 5.0 % Error in Z Prediction -0.050 Predicted X = 0.006 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -2.149 StdErr = 0.058 nFit = 5 nDrop = 0 Predicted Z = 4.334 StdErr = 0.040 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 25, reference by 20 20 Tracking image shifted 0.002, 0.018 um; position 0.004, -2.167 Drift reached 0.4 A/sec after 7.0 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.04 nm/sec Tracking image shifted -0.001, 0.001 um; position 0.006, -2.168 Running script # 14 0.5 1.1 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.1 nm on specimen axes Rate = 2.03997 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_047_-62.0_Oct22_13.50.40.tif Record image shifted 0.001, 0.019 um Tracking reference shifted by 53.9,-6.5 pixels, 0.001,0.019 um Saved Z = 47 at 33.76 min Tilt = -62.00 Mean = 102 Exp = 2.980 X/Y/Z = 0.005 -2.187 4.298 Errors in X/Y Prediction -0.000 -0.038 um, 0.0 2.9 % Error in Z Prediction -0.036 Predicted X = 0.002 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.109 StdErr = 0.118 nFit = 5 nDrop = 0 Predicted Z = 2.237 StdErr = 0.029 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 30, reference by 20 20 Tracking image shifted -0.017, -0.076 um; position 0.019, -0.033 Drift reached 4.9 A/sec after 5.6 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.30 nm/sec Trimming A by 20 30, reference by 20 20 Tracking image shifted -0.003, -0.012 um; position 0.023, -0.019 Running script # 14 Trimming A by 27 20 45 20, reference by 27 20 45 20 Trimming A by 26 20 40 20, reference by 27 20 45 20 -5.8 2.1 unbinned pixels; 1.4 1.7 nm along two shift axes; -0.6 -2.1 nm on specimen axes Rate = 10.84126 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_048_34.0_Oct22_13.51.34.tif Record image shifted 0.001, -0.015 um Tracking reference shifted by -44.0,1.4 pixels, 0.001,-0.015 um Saved Z = 48 at 34.72 min Tilt = 34.00 Mean = 105 Exp = 1.941 X/Y/Z = 0.022 -0.001 2.206 Errors in X/Y Prediction 0.021 0.108 um, 1.6 8.3 % Error in Z Prediction -0.031 Predicted X = 0.052 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = 0.051 StdErr = 0.096 nFit = 5 nDrop = 0 Predicted Z = 2.102 StdErr = 0.036 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 45, reference by 20 30 Tracking image shifted 0.047, 0.181 um; position 0.005, -0.129 Drift reached 2.9 A/sec after 5.7 sec, going on Running script # 14 Trimming A by 21 20 45 30, reference by 21 20 45 30 Trimming A by 31 20 40 20, reference by 21 20 45 30 -2.3 4.0 unbinned pixels; 1.6 0.3 nm along two shift axes; -1.3 -0.9 nm on specimen axes Rate = 8.12505 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_049_36.0_Oct22_13.52.14.tif Record image shifted 0.003, -0.017 um Tracking reference shifted by -48.7,-5.5 pixels, 0.003,-0.017 um Saved Z = 49 at 35.32 min Tilt = 36.00 Mean = 107 Exp = 2.024 X/Y/Z = 0.004 -0.110 2.102 Errors in X/Y Prediction -0.048 -0.161 um, 3.7 12.4 % Predicted X = 0.012 StdErr = 0.029 nFit = 3 nDrop = 0 Predicted Y = -0.179 StdErr = 0.104 nFit = 5 nDrop = 0 Predicted Z = 1.984 StdErr = 0.041 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 45, reference by 20 30 Tracking image shifted 0.007, -0.076 um; position 0.005, -0.104 Drift reached 3.5 A/sec after 5.7 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.31 nm/sec Trimming A by 20 45, reference by 20 30 Tracking image shifted -0.002, -0.012 um; position 0.007, -0.089 Running script # 14 Trimming A by 27 20 45 25, reference by 27 20 45 25 Trimming A by 27 20 45 25, reference by 27 20 45 25 -6.0 3.2 unbinned pixels; 1.8 1.6 nm along two shift axes; -1.0 -2.2 nm on specimen axes Rate = 11.98228 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_050_38.0_Oct22_13.53.02.tif Trimming A by 20 25, reference by 20 20 Record image shifted -0.002, -0.015 um Tracking reference shifted by -41.9,10.6 pixels, -0.002,-0.015 um Saved Z = 50 at 36.12 min Tilt = 38.00 Mean = 106 Exp = 2.081 X/Y/Z = 0.011 -0.072 1.980 Errors in X/Y Prediction -0.001 0.107 um, 0.1 8.2 % Error in Z Prediction -0.004 Predicted X = 0.001 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = -0.087 StdErr = 0.117 nFit = 5 nDrop = 0 Predicted Z = 1.848 StdErr = 0.019 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 45, reference by 20 35 Tracking image shifted -0.005, 0.041 um; position 0.006, -0.128 Drift reached 1.7 A/sec after 6.0 sec, going on Running script # 14 Trimming A by 55 21 45 20, reference by 55 21 45 20 Trimming A by 21 20 50 30, reference by 55 21 45 20 -2.1 2.1 unbinned pixels; 0.9 0.4 nm along two shift axes; -0.7 -0.8 nm on specimen axes Rate = 5.17336 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_051_40.0_Oct22_13.53.38.tif Trimming A by 20 25, reference by 20 25 Record image shifted -0.002, -0.017 um Tracking reference shifted by -48.3,9.3 pixels, -0.002,-0.017 um Saved Z = 51 at 36.72 min Tilt = 40.00 Mean = 106 Exp = 2.152 X/Y/Z = 0.009 -0.109 1.848 Errors in X/Y Prediction 0.008 -0.022 um, 0.6 1.7 % Predicted X = 0.013 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.137 StdErr = 0.114 nFit = 5 nDrop = 0 Predicted Z = 1.724 StdErr = 0.021 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 55, reference by 20 35 Tracking image shifted 0.001, 0.014 um; position 0.012, -0.152 Drift reached 4.5 A/sec after 6.0 sec, going on Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.29 nm/sec Trimming A by 20 50, reference by 20 35 Tracking image shifted -0.002, -0.013 um; position 0.014, -0.136 Running script # 14 Trimming A by 22 20 55 35, reference by 22 20 55 35 Trimming A by 55 21 45 30, reference by 22 20 55 35 -5.1 1.2 unbinned pixels; 1.0 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 9.21841 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_052_42.0_Oct22_13.54.28.tif Trimming A by 30 30, reference by 20 25 Record image shifted 0.001, -0.016 um Tracking reference shifted by -44.4,-0.1 pixels, 0.001,-0.016 um Saved Z = 52 at 37.54 min Tilt = 42.00 Mean = 106 Exp = 2.230 X/Y/Z = 0.013 -0.118 1.798 Errors in X/Y Prediction -0.000 0.019 um, 0.0 1.4 % Error in Z Prediction 0.074 Predicted X = 0.005 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Y = -2.268 StdErr = 0.045 nFit = 6 nDrop = 0 Predicted Z = 4.239 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 45, reference by 20 20 Tracking image shifted -0.008, 0.001 um; position 0.013, -2.269 Drift reached 1.5 A/sec after 7.4 sec, going on Running script # 14 Trimming A by 22 20 45 20, reference by 22 20 45 20 Trimming A by 22 20 50 20, reference by 22 20 45 20 2.8 0.5 unbinned pixels; 0.2 1.0 nm along two shift axes; -0.2 1.0 nm on specimen axes Rate = 4.97101 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_053_-64.0_Oct22_13.55.13.tif Trimming A by 20 30, reference by 20 20 Record image shifted -0.001, 0.015 um Tracking reference shifted by 41.8,-2.0 pixels, -0.001,0.015 um Saved Z = 53 at 38.31 min Tilt = -64.00 Mean = 100 Exp = 3.214 X/Y/Z = 0.014 -2.286 4.239 Errors in X/Y Prediction 0.009 -0.017 um, 0.7 1.3 % Predicted X = 0.026 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = -2.402 StdErr = 0.029 nFit = 6 nDrop = 0 Predicted Z = 4.178 StdErr = 0.016 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 50, reference by 20 20 Tracking image shifted 0.019, -0.023 um; position 0.007, -2.379 Drift reached 0.8 A/sec after 7.7 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.03 nm/sec Trimming A by 20 45, reference by 20 20 Tracking image shifted -0.002, -0.000 um; position 0.009, -2.379 Running script # 14 Trimming A by 22 20 55 20, reference by 22 20 55 20 Trimming A by 22 20 55 20, reference by 22 20 55 20 0.2 0.7 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.27622 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 30 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_054_-66.0_Oct22_13.56.10.tif Trimming A by 20 20, reference by 20 25 Record image shifted 0.001, 0.019 um Tracking reference shifted by 52.9,-8.7 pixels, 0.001,0.019 um Saved Z = 54 at 39.27 min Tilt = -65.99 Mean = 100 Exp = 3.548 X/Y/Z = 0.008 -2.398 4.164 Errors in X/Y Prediction -0.018 0.004 um, 1.4 0.3 % Error in Z Prediction -0.014 Predicted X = 0.004 StdErr = 0.015 nFit = 4 nDrop = 0 Predicted Y = -2.494 StdErr = 0.019 nFit = 5 nDrop = 1 Predicted Z = 4.095 StdErr = 0.008 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Trimming A by 20 50, reference by 20 45 Tracking image shifted -0.004, -0.069 um; position 0.008, -2.425 Drift reached 0.8 A/sec after 8.2 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.03 nm/sec Trimming A by 20 60, reference by 20 45 Tracking image shifted -0.001, 0.000 um; position 0.010, -2.425 Running script # 14 Trimming A by 55 21 50 20, reference by 55 21 50 20 Trimming A by 22 20 50 20, reference by 55 21 50 20 0.3 0.5 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.0937 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_055_-68.0_Oct22_13.57.09.tif Trimming A by 20 35, reference by 20 20 Record image shifted 0.001, 0.024 um Tracking reference shifted by 67.9,-8.0 pixels, 0.001,0.024 um Saved Z = 55 at 40.25 min Tilt = -68.00 Mean = 97 Exp = 3.902 X/Y/Z = 0.010 -2.449 4.122 Errors in X/Y Prediction 0.005 0.044 um, 0.4 3.4 % Error in Z Prediction 0.027 Predicted X = 0.013 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.090 StdErr = 0.091 nFit = 5 nDrop = 0 Predicted Z = 1.672 StdErr = 0.039 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 60, reference by 20 50 Tracking image shifted -0.011, 0.072 um; position 0.024, -0.162 Drift reached 2.0 A/sec after 6.2 sec, going on Running script # 14 Trimming A by 23 20 65 30, reference by 23 20 65 30 Trimming A by 23 20 65 30, reference by 23 20 65 30 -4.7 2.2 unbinned pixels; 1.3 1.3 nm along two shift axes; -0.6 -1.7 nm on specimen axes Rate = 9.18555 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_056_44.0_Oct22_13.57.54.tif Trimming A by 37 70, reference by 40 40 Record image shifted 0.002, -0.020 um Tracking reference shifted by -56.0,-0.6 pixels, 0.002,-0.020 um Saved Z = 56 at 40.97 min Tilt = 44.00 Mean = 103 Exp = 2.325 X/Y/Z = 0.023 -0.139 1.672 Errors in X/Y Prediction 0.010 -0.050 um, 0.8 3.8 % Predicted X = 0.039 StdErr = 0.001 nFit = 4 nDrop = 0 Predicted Y = -0.186 StdErr = 0.044 nFit = 5 nDrop = 0 Predicted Z = 1.563 StdErr = 0.043 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 20, reference by 20 50 Tracking image shifted 0.023, -0.002 um; position 0.016, -0.185 Drift reached 0.2 A/sec after 6.5 sec, going on Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.02 nm/sec Trimming A by 20 20, reference by 20 50 Tracking image shifted -0.000, -0.000 um; position 0.016, -0.184 Running script # 14 -0.2 0.6 unbinned pixels; 0.2 0.0 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.11908 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_057_46.0_Oct22_14.03.06.tif Trimming A by 60 70, reference by 46 70 Record image shifted -0.000, -0.012 um Tracking reference shifted by -32.8,3.2 pixels, -0.000,-0.012 um Saved Z = 57 at 46.19 min Tilt = 46.00 Mean = 114 Exp = 2.477 X/Y/Z = 0.016 -0.173 1.647 Errors in X/Y Prediction -0.023 0.014 um, 1.7 1.1 % Error in Z Prediction 0.085 Predicted X = 0.010 StdErr = 0.015 nFit = 4 nDrop = 0 Predicted Y = -0.194 StdErr = 0.023 nFit = 5 nDrop = 0 Predicted Z = 1.559 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.018, -0.022 um; position 0.028, -0.172 Drift reached 4.0 A/sec after 6.2 sec, going on Running script # 14 -7.4 1.7 unbinned pixels; 1.4 2.2 nm along two shift axes; -0.4 -2.6 nm on specimen axes Rate = 13.32495 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_058_48.0_Oct22_14.03.43.tif Trimming A by 22 110, reference by 70 70 Record image shifted -0.001, -0.027 um Tracking reference shifted by -77.4,9.9 pixels, -0.001,-0.027 um Saved Z = 58 at 46.81 min Tilt = 48.00 Mean = 104 Exp = 2.398 X/Y/Z = 0.030 -0.139 1.559 Errors in X/Y Prediction 0.020 0.054 um, 1.6 4.2 % Predicted X = 0.030 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = -0.137 StdErr = 0.032 nFit = 6 nDrop = 0 Predicted Z = 1.476 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.046 um; position 0.030, -0.183 Drift reached 1.5 A/sec after 6.5 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.08 nm/sec Tracking image shifted -0.003, -0.004 um; position 0.034, -0.180 Running script # 14 -0.9 1.7 unbinned pixels; 0.6 0.1 nm along two shift axes; -0.6 -0.4 nm on specimen axes Rate = 3.27709 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo3_L1G1_059_50.0_Oct22_14.04.36.tif Trimming A by 22 115, reference by 24 115 Record image shifted 0.001, -0.012 um Tracking reference shifted by -34.7,1.4 pixels, 0.001,-0.012 um Saved Z = 59 at 47.68 min Tilt = 50.00 Mean = 103 Exp = 2.542 X/Y/Z = 0.034 -0.167 1.448 2020/10/22 14:04 D:\Users\Ben\201022_Jans_cells\SEM\tomo3_L1G1.st 0 60 3838 3710 1708 -10.0 50.0 2.00 REG 47.7 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Measured defocus = -3.21 microns changed by -1.29 to target drift = 0.08 nm/sec Need regular tracking - Need autofocus Tracking image shifted 0.000, -0.000 um; position -0.000, 0.000 Drift reached 0.3 A/sec after 5.2 sec, going on Measured defocus = -5.34 microns changed by 0.84 to target drift = 0.03 nm/sec Tracking image shifted 0.000, -0.001 um; position -0.000, 0.002 Running script # 14 -1.0 -0.7 unbinned pixels; 0.1 0.4 nm along two shift axes; 0.3 -0.3 nm on specimen axes Rate = 2.18156 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_000_-10.0_Oct22_14.16.59.tif Record image shifted -0.005, -0.011 um Tracking reference shifted by -29.4,17.0 pixels, -0.005,-0.011 um Saved Z = 0 at -0.00 min Tilt = -10.00 Mean = 104 Exp = 1.680 X/Y/Z = 0.004 0.013 4.053 Need regular tracking - Need autofocus Trimming A by 20 30, reference by 20 20 Tracking image shifted -0.005, 0.033 um; position 0.010, -0.020 Drift reached 1.5 A/sec after 5.2 sec, going on Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.06 nm/sec Trimming A by 20 25, reference by 20 20 Tracking image shifted 0.000, 0.002 um; position 0.009, -0.023 Running script # 14 Trimming A by 20 20 20 25, reference by 20 20 20 25 Trimming A by 20 20 20 25, reference by 20 20 20 25 0.4 -0.0 unbinned pixels; 0.0 0.1 nm along two shift axes; -0.0 0.1 nm on specimen axes Rate = 0.68884 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_001_-12.0_Oct22_14.17.40.tif Record image shifted -0.000, 0.006 um Tracking reference shifted by 18.0,-1.2 pixels, -0.000,0.006 um Saved Z = 1 at 0.77 min Tilt = -12.00 Mean = 100 Exp = 1.613 X/Y/Z = 0.010 -0.029 4.123 Predicted X = 0.015 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.071 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 4.193 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 35, reference by 20 25 Tracking image shifted 0.009, 0.014 um; position 0.006, -0.085 Drift reached 0.9 A/sec after 5.3 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.06 nm/sec Trimming A by 20 30, reference by 20 25 Tracking image shifted 0.001, -0.002 um; position 0.006, -0.082 Running script # 14 Trimming A by 20 20 20 30, reference by 20 20 20 30 Trimming A by 20 20 20 30, reference by 20 20 20 30 -0.9 -0.1 unbinned pixels; 0.1 0.3 nm along two shift axes; 0.1 -0.3 nm on specimen axes Rate = 1.63056 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_002_-14.0_Oct22_14.18.27.tif Record image shifted 0.000, 0.004 um Tracking reference shifted by 12.0,-1.4 pixels, 0.000,0.004 um Saved Z = 2 at 1.49 min Tilt = -14.00 Mean = 100 Exp = 1.616 X/Y/Z = 0.005 -0.086 4.217 Errors in X/Y Prediction -0.009 -0.015 um, 0.7 1.1 % Error in Z Prediction 0.024 Need regular tracking - Need autofocus Trimming A by 20 35, reference by 20 20 Tracking image shifted -0.021, 0.155 um; position 0.026, -0.142 Drift reached 3.1 A/sec after 5.3 sec, going on Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.13 nm/sec Tracking image shifted 0.003, 0.006 um; position 0.023, -0.150 Running script # 14 1.5 -0.7 unbinned pixels; 0.4 0.4 nm along two shift axes; 0.2 0.6 nm on specimen axes Rate = 2.93692 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_003_-8.0_Oct22_14.19.14.tif Record image shifted 0.001, -0.003 um Tracking reference shifted by -8.8,-1.0 pixels, 0.001,-0.003 um Saved Z = 3 at 2.24 min Tilt = -8.00 Mean = 100 Exp = 1.613 X/Y/Z = 0.022 -0.148 3.949 Need regular tracking - Need autofocus Tracking image shifted -0.004, -0.093 um; position 0.026, -0.054 Drift reached 1.2 A/sec after 5.1 sec, going on Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.08 nm/sec Tracking image shifted -0.000, -0.004 um; position 0.027, -0.049 Running script # 14 -1.4 -0.1 unbinned pixels; 0.1 0.5 nm along two shift axes; 0.1 -0.5 nm on specimen axes Rate = 2.49112 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_004_-6.0_Oct22_14.19.57.tif Record image shifted 0.000, -0.008 um Tracking reference shifted by -21.9,0.8 pixels, 0.000,-0.008 um Saved Z = 4 at 2.97 min Tilt = -6.00 Mean = 100 Exp = 1.607 X/Y/Z = 0.027 -0.041 3.871 Predicted X = 0.007 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.133 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Z = 4.295 StdErr = 0.018 nFit = 3 nDrop = 0 Drift reached 0.3 A/sec after 4.7 sec, going on Running script # 14 Trimming A by 20 20 20 25, reference by 20 20 20 25 Trimming A by 20 20 20 25, reference by 20 20 20 25 -1.6 -0.1 unbinned pixels; 0.1 0.5 nm along two shift axes; 0.1 -0.5 nm on specimen axes Rate = 2.73639 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_005_-16.0_Oct22_14.20.28.tif Record image shifted -0.028, -0.028 um Saved Z = 5 at 3.49 min Tilt = -16.00 Mean = 98 Exp = 1.619 X/Y/Z = 0.035 -0.104 4.295 Errors in X/Y Prediction 0.028 0.029 um, 2.1 2.2 % Predicted X = 0.042 StdErr = 0.025 nFit = 3 nDrop = 0 Predicted Y = -0.148 StdErr = 0.029 nFit = 3 nDrop = 0 Predicted Z = 4.377 StdErr = 0.024 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 45, reference by 20 30 Tracking image shifted 0.009, 0.016 um; position 0.033, -0.165 Drift reached 0.7 A/sec after 5.1 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.03 nm/sec Trimming A by 20 40, reference by 20 30 Tracking image shifted 0.001, 0.004 um; position 0.032, -0.169 Running script # 14 Trimming A by 22 25 20 40, reference by 22 25 20 40 Trimming A by 22 25 20 40, reference by 22 25 20 40 0.3 -0.2 unbinned pixels; 0.1 0.1 nm along two shift axes; 0.0 0.1 nm on specimen axes Rate = 0.64982 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_006_-18.0_Oct22_14.21.10.tif Record image shifted 0.001, 0.010 um Tracking reference shifted by 29.2,-4.0 pixels, 0.001,0.010 um Saved Z = 6 at 4.18 min Tilt = -18.00 Mean = 100 Exp = 1.646 X/Y/Z = 0.032 -0.179 4.380 Errors in X/Y Prediction -0.010 -0.031 um, 0.8 2.4 % Error in Z Prediction 0.003 Predicted X = 0.031 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.065 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.793 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, 0.027 um; position 0.029, 0.038 Drift reached 2.9 A/sec after 5.2 sec, going on Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.13 nm/sec Tracking image shifted 0.001, 0.006 um; position 0.028, 0.030 Running script # 14 2.5 0.0 unbinned pixels; 0.3 0.8 nm along two shift axes; -0.1 0.9 nm on specimen axes Rate = 4.41175 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_007_-4.0_Oct22_14.21.55.tif Record image shifted 0.001, -0.004 um Tracking reference shifted by -10.2,-0.8 pixels, 0.001,-0.004 um Saved Z = 7 at 4.93 min Tilt = -4.00 Mean = 100 Exp = 1.611 X/Y/Z = 0.027 0.033 3.789 Errors in X/Y Prediction -0.004 -0.032 um, 0.3 2.5 % Error in Z Prediction -0.004 Predicted X = 0.030 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.128 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Z = 3.710 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, 0.055 um; position 0.023, 0.074 Drift reached 1.6 A/sec after 5.2 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.09 nm/sec Tracking image shifted 0.001, -0.005 um; position 0.022, 0.079 Running script # 14 -1.4 0.1 unbinned pixels; 0.2 0.5 nm along two shift axes; 0.0 -0.5 nm on specimen axes Rate = 2.48824 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_008_-2.0_Oct22_14.22.39.tif Record image shifted -0.001, -0.007 um Tracking reference shifted by -20.2,3.9 pixels, -0.001,-0.007 um Saved Z = 8 at 5.66 min Tilt = -2.00 Mean = 100 Exp = 1.613 X/Y/Z = 0.023 0.087 3.736 Errors in X/Y Prediction -0.008 -0.041 um, 0.6 3.2 % Error in Z Prediction 0.026 Predicted X = 0.051 StdErr = 0.025 nFit = 3 nDrop = 0 Predicted Y = -0.216 StdErr = 0.042 nFit = 3 nDrop = 0 Predicted Z = 4.467 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Trimming A by 20 60, reference by 25 40 Tracking image shifted 0.016, 0.063 um; position 0.034, -0.279 Drift reached 0.9 A/sec after 5.2 sec, going on Running script # 14 Trimming A by 22 25 20 45, reference by 22 25 20 45 Trimming A by 22 25 20 45, reference by 22 25 20 45 -0.3 0.4 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.1 -0.1 nm on specimen axes Rate = 0.95335 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_009_-20.0_Oct22_14.23.12.tif Record image shifted 0.001, 0.004 um Tracking reference shifted by 11.1,-5.1 pixels, 0.001,0.004 um Saved Z = 9 at 6.21 min Tilt = -20.00 Mean = 99 Exp = 1.651 X/Y/Z = 0.033 -0.283 4.467 Errors in X/Y Prediction -0.017 -0.067 um, 1.3 5.1 % Predicted X = 0.032 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.437 StdErr = 0.019 nFit = 4 nDrop = 0 Predicted Z = 4.552 StdErr = 0.014 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 35, reference by 25 40 Tracking image shifted 0.005, -0.090 um; position 0.027, -0.347 Drift reached 2.0 A/sec after 5.2 sec, going on Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.06 nm/sec Trimming A by 20 50, reference by 25 40 Tracking image shifted -0.000, -0.001 um; position 0.028, -0.347 Running script # 14 Trimming A by 23 25 20 50, reference by 23 25 20 50 Trimming A by 23 25 20 50, reference by 23 25 20 50 0.6 0.2 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 1.10226 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_010_-22.0_Oct22_14.23.54.tif Record image shifted -0.002, 0.010 um Tracking reference shifted by 27.4,2.1 pixels, -0.002,0.010 um Saved Z = 10 at 6.91 min Tilt = -22.00 Mean = 99 Exp = 1.663 X/Y/Z = 0.030 -0.357 4.455 Errors in X/Y Prediction -0.002 0.080 um, 0.2 6.1 % Error in Z Prediction -0.097 Predicted X = 0.021 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.155 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Z = 3.663 StdErr = 0.022 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.007, 0.013 um; position 0.029, 0.142 Drift reached 2.9 A/sec after 5.2 sec, going on Running script # 14 2.9 -0.0 unbinned pixels; 0.4 1.0 nm along two shift axes; -0.1 1.0 nm on specimen axes Rate = 5.11575 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_011_0.0_Oct22_14.24.28.tif Record image shifted 0.000, 0.002 um Tracking reference shifted by 4.2,-1.7 pixels, 0.000,0.002 um Saved Z = 11 at 7.48 min Tilt = 0.00 Mean = 100 Exp = 1.617 X/Y/Z = 0.028 0.138 3.663 Errors in X/Y Prediction 0.007 -0.017 um, 0.5 1.3 % Predicted X = 0.027 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = 0.191 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Z = 3.596 StdErr = 0.028 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.033 um; position 0.021, 0.158 Drift reached 1.5 A/sec after 5.2 sec, going on Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.12 nm/sec Tracking image shifted 0.000, -0.006 um; position 0.021, 0.165 Running script # 14 -1.8 0.7 unbinned pixels; 0.5 0.5 nm along two shift axes; -0.2 -0.6 nm on specimen axes Rate = 3.36983 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_012_2.0_Oct22_14.25.12.tif Record image shifted -0.000, -0.010 um Tracking reference shifted by -28.3,3.2 pixels, -0.000,-0.010 um Saved Z = 12 at 8.21 min Tilt = 2.00 Mean = 100 Exp = 1.619 X/Y/Z = 0.021 0.175 3.567 Errors in X/Y Prediction -0.006 -0.016 um, 0.4 1.2 % Error in Z Prediction -0.028 Predicted X = 0.029 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.489 StdErr = 0.046 nFit = 5 nDrop = 0 Predicted Z = 4.529 StdErr = 0.057 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 55, reference by 25 50 Tracking image shifted -0.002, 0.000 um; position 0.031, -0.489 Drift reached 0.3 A/sec after 5.2 sec, going on Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.09 nm/sec Trimming A by 20 55, reference by 25 50 Tracking image shifted -0.001, -0.001 um; position 0.032, -0.488 Running script # 14 Trimming A by 23 25 20 55, reference by 23 25 20 55 Trimming A by 23 25 20 55, reference by 23 25 20 55 -0.4 0.4 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.1 -0.2 nm on specimen axes Rate = 1.00049 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_013_-24.0_Oct22_14.25.56.tif Record image shifted 0.000, 0.005 um Tracking reference shifted by 14.3,-1.4 pixels, 0.000,0.005 um Saved Z = 13 at 8.95 min Tilt = -24.00 Mean = 99 Exp = 1.678 X/Y/Z = 0.032 -0.493 4.497 Errors in X/Y Prediction 0.003 -0.004 um, 0.2 0.3 % Error in Z Prediction -0.032 Predicted X = 0.030 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.615 StdErr = 0.035 nFit = 5 nDrop = 0 Predicted Z = 4.535 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 25 45, reference by 25 55 Tracking image shifted 0.002, -0.090 um; position 0.029, -0.525 Drift reached 1.9 A/sec after 5.2 sec, going on Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.08 nm/sec Trimming A by 20 60, reference by 25 55 Tracking image shifted -0.001, 0.002 um; position 0.030, -0.528 Running script # 14 Trimming A by 24 25 20 60, reference by 24 25 20 60 Trimming A by 24 25 20 60, reference by 24 25 20 60 0.8 0.4 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.49136 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_014_-26.0_Oct22_14.26.38.tif Record image shifted -0.000, 0.007 um Tracking reference shifted by 19.9,-1.3 pixels, -0.000,0.007 um Saved Z = 14 at 9.65 min Tilt = -26.00 Mean = 99 Exp = 1.694 X/Y/Z = 0.030 -0.535 4.543 Errors in X/Y Prediction 0.000 0.080 um, 0.0 6.2 % Error in Z Prediction 0.009 Predicted X = 0.019 StdErr = 0.015 nFit = 4 nDrop = 0 Predicted Y = 0.207 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Z = 3.497 StdErr = 0.028 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.012, -0.048 um; position 0.031, 0.255 Drift reached 2.8 A/sec after 5.2 sec, going on Running script # 14 2.2 1.0 unbinned pixels; 0.1 0.8 nm along two shift axes; -0.4 0.7 nm on specimen axes Rate = 4.21077 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_015_4.0_Oct22_14.27.13.tif Record image shifted -0.001, 0.000 um Tracking reference shifted by 0.7,2.2 pixels, -0.001,0.000 um Saved Z = 15 at 10.23 min Tilt = 4.00 Mean = 100 Exp = 1.627 X/Y/Z = 0.033 0.253 3.497 Errors in X/Y Prediction 0.014 0.046 um, 1.0 3.5 % Predicted X = 0.039 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Y = 0.312 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 3.421 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.015, 0.087 um; position 0.024, 0.225 Drift reached 5.0 A/sec after 5.2 sec, going on Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.31 nm/sec Tracking image shifted -0.000, -0.017 um; position 0.025, 0.244 Running script # 14 -5.5 1.6 unbinned pixels; 1.2 1.6 nm along two shift axes; -0.4 -2.0 nm on specimen axes Rate = 9.99861 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_016_6.0_Oct22_14.27.57.tif Record image shifted 0.000, -0.010 um Tracking reference shifted by -27.9,1.8 pixels, 0.000,-0.010 um Saved Z = 16 at 10.96 min Tilt = 6.00 Mean = 99 Exp = 1.635 X/Y/Z = 0.025 0.256 3.429 Errors in X/Y Prediction -0.014 -0.056 um, 1.1 4.3 % Error in Z Prediction 0.008 Predicted X = 0.032 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Y = -0.636 StdErr = 0.047 nFit = 6 nDrop = 0 Predicted Z = 4.580 StdErr = 0.006 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 25 60, reference by 25 60 Tracking image shifted -0.004, -0.015 um; position 0.036, -0.621 Drift reached 0.3 A/sec after 5.2 sec, going on Running script # 14 Trimming A by 20 25 20 65, reference by 20 25 20 65 Trimming A by 20 25 20 65, reference by 20 25 20 65 -0.3 0.7 unbinned pixels; 0.3 0.0 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.37022 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_017_-28.0_Oct22_14.28.31.tif Record image shifted -0.000, 0.005 um Tracking reference shifted by 13.6,-0.6 pixels, -0.000,0.005 um Saved Z = 17 at 11.61 min Tilt = -28.00 Mean = 99 Exp = 1.714 X/Y/Z = 0.037 -0.626 4.580 Errors in X/Y Prediction 0.005 0.010 um, 0.4 0.8 % Predicted X = 0.043 StdErr = 0.008 nFit = 4 nDrop = 0 Predicted Y = -0.692 StdErr = 0.043 nFit = 6 nDrop = 0 Predicted Z = 4.621 StdErr = 0.007 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 21 70, reference by 25 60 Tracking image shifted 0.007, -0.020 um; position 0.036, -0.672 Drift reached 1.5 A/sec after 5.2 sec, going on Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.07 nm/sec Trimming A by 21 70, reference by 25 60 Tracking image shifted -0.001, 0.001 um; position 0.037, -0.673 Running script # 14 Trimming A by 20 20 20 70, reference by 20 20 20 70 Trimming A by 20 20 20 70, reference by 20 20 20 70 0.5 0.6 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.37958 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_018_-30.0_Oct22_14.29.18.tif Record image shifted 0.000, 0.006 um Tracking reference shifted by 18.0,-2.6 pixels, 0.000,0.006 um Saved Z = 18 at 12.31 min Tilt = -30.00 Mean = 99 Exp = 1.738 X/Y/Z = 0.037 -0.680 4.588 Errors in X/Y Prediction -0.006 0.012 um, 0.5 0.9 % Error in Z Prediction -0.033 Predicted X = 0.027 StdErr = 0.025 nFit = 4 nDrop = 0 Predicted Y = 0.298 StdErr = 0.034 nFit = 6 nDrop = 0 Predicted Z = 3.348 StdErr = 0.019 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, 0.021 um; position 0.029, 0.277 Drift reached 0.7 A/sec after 5.2 sec, going on Running script # 14 -0.9 1.4 unbinned pixels; 0.6 0.1 nm along two shift axes; -0.5 -0.3 nm on specimen axes Rate = 2.92814 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_019_8.0_Oct22_14.29.54.tif Record image shifted 0.000, -0.004 um Tracking reference shifted by -10.1,0.8 pixels, 0.000,-0.004 um Saved Z = 19 at 12.92 min Tilt = 8.00 Mean = 99 Exp = 1.644 X/Y/Z = 0.029 0.281 3.348 Errors in X/Y Prediction 0.003 -0.017 um, 0.2 1.3 % Predicted X = 0.026 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = 0.295 StdErr = 0.033 nFit = 6 nDrop = 0 Predicted Z = 3.271 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, 0.014 um; position 0.033, 0.280 Drift reached 1.4 A/sec after 5.2 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.12 nm/sec Tracking image shifted -0.002, -0.005 um; position 0.035, 0.286 Running script # 14 -1.9 1.4 unbinned pixels; 0.7 0.5 nm along two shift axes; -0.4 -0.7 nm on specimen axes Rate = 4.10962 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_020_10.0_Oct22_14.30.39.tif Record image shifted 0.000, -0.003 um Tracking reference shifted by -9.8,-0.2 pixels, 0.000,-0.003 um Saved Z = 20 at 13.66 min Tilt = 10.00 Mean = 99 Exp = 1.653 X/Y/Z = 0.035 0.291 3.255 Errors in X/Y Prediction 0.010 -0.004 um, 0.7 0.3 % Error in Z Prediction -0.016 Predicted X = 0.041 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.715 StdErr = 0.054 nFit = 5 nDrop = 0 Predicted Z = 4.635 StdErr = 0.017 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 70, reference by 21 70 Tracking image shifted -0.015, 0.022 um; position 0.056, -0.736 Trimming A by 20 20 20 25, reference by 20 20 20 25 Drift reached 1.5 A/sec after 5.2 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.06 nm/sec Trimming A by 21 75, reference by 21 70 Tracking image shifted -0.000, 0.002 um; position 0.057, -0.739 Running script # 14 Trimming A by 21 21 20 75, reference by 21 21 20 75 Trimming A by 21 21 20 75, reference by 21 21 20 75 0.5 0.6 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.35031 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_021_-32.0_Oct22_14.31.25.tif Record image shifted 0.000, 0.006 um Tracking reference shifted by 17.2,-2.2 pixels, 0.000,0.006 um Saved Z = 21 at 14.43 min Tilt = -32.00 Mean = 98 Exp = 1.761 X/Y/Z = 0.057 -0.745 4.593 Errors in X/Y Prediction 0.016 -0.031 um, 1.2 2.3 % Error in Z Prediction -0.041 Predicted X = 0.064 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = -0.816 StdErr = 0.033 nFit = 5 nDrop = 0 Predicted Z = 4.635 StdErr = 0.023 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 21 80, reference by 21 75 Tracking image shifted 0.010, -0.018 um; position 0.053, -0.798 Trimming A by 21 25 20 30, reference by 21 25 20 30 Drift reached 1.0 A/sec after 5.4 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.05 nm/sec Trimming A by 20 75, reference by 21 75 Tracking image shifted -0.001, 0.003 um; position 0.054, -0.801 Running script # 14 Trimming A by 20 20 55 70, reference by 20 20 55 70 Trimming A by 20 20 55 70, reference by 20 20 55 70 0.7 0.5 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.51191 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_022_-34.0_Oct22_14.32.08.tif Record image shifted 0.000, 0.007 um Tracking reference shifted by 18.5,-2.0 pixels, 0.000,0.007 um Saved Z = 22 at 15.16 min Tilt = -34.00 Mean = 98 Exp = 1.792 X/Y/Z = 0.054 -0.808 4.623 Errors in X/Y Prediction -0.010 0.007 um, 0.7 0.6 % Error in Z Prediction -0.012 Predicted X = 0.040 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.282 StdErr = 0.031 nFit = 6 nDrop = 0 Predicted Z = 3.181 StdErr = 0.015 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.012, -0.033 um; position 0.052, 0.315 Drift reached 4.5 A/sec after 5.2 sec, going on Running script # 14 -8.2 2.9 unbinned pixels; 1.9 2.4 nm along two shift axes; -0.8 -3.0 nm on specimen axes Rate = 15.26972 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_023_12.0_Oct22_14.32.45.tif Record image shifted -0.002, -0.015 um Tracking reference shifted by -42.0,8.0 pixels, -0.002,-0.015 um Saved Z = 23 at 15.76 min Tilt = 12.00 Mean = 99 Exp = 1.669 X/Y/Z = 0.055 0.335 3.181 Errors in X/Y Prediction 0.015 0.053 um, 1.2 4.1 % Predicted X = 0.066 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.377 StdErr = 0.019 nFit = 5 nDrop = 0 Predicted Z = 3.102 StdErr = 0.017 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, 0.087 um; position 0.053, 0.289 Drift reached 4.1 A/sec after 5.3 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.14 nm/sec Tracking image shifted -0.001, 0.007 um; position 0.055, 0.281 Running script # 14 -0.2 1.0 unbinned pixels; 0.4 0.0 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.82525 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_024_14.0_Oct22_14.33.29.tif Record image shifted 0.002, 0.003 um Tracking reference shifted by 7.2,-5.2 pixels, 0.002,0.003 um Saved Z = 24 at 16.51 min Tilt = 14.00 Mean = 99 Exp = 1.684 X/Y/Z = 0.054 0.278 3.088 Errors in X/Y Prediction -0.012 -0.099 um, 0.9 7.6 % Error in Z Prediction -0.014 Predicted X = 0.067 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = -0.875 StdErr = 0.024 nFit = 6 nDrop = 0 Predicted Z = 4.639 StdErr = 0.010 nFit = 4 nDrop = 1 Need regular tracking - Trimming A by 20 80, reference by 20 75 Tracking image shifted 0.001, 0.042 um; position 0.066, -0.917 Trimming A by 21 25 20 35, reference by 20 20 20 40 Drift reached 0.9 A/sec after 5.6 sec, going on Running script # 14 Trimming A by 20 20 60 65, reference by 20 20 60 65 Trimming A by 20 20 55 65, reference by 20 20 60 65 0.2 0.9 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.3 0.0 nm on specimen axes Rate = 1.61547 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_025_-36.0_Oct22_14.34.05.tif Record image shifted 0.000, 0.005 um Tracking reference shifted by 13.8,-1.4 pixels, 0.000,0.005 um Saved Z = 25 at 17.11 min Tilt = -36.00 Mean = 98 Exp = 1.828 X/Y/Z = 0.066 -0.922 4.639 Errors in X/Y Prediction -0.000 -0.047 um, 0.0 3.6 % Predicted X = 0.068 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -1.030 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 4.658 StdErr = 0.012 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Trimming A by 21 80, reference by 20 75 Tracking image shifted 0.006, -0.054 um; position 0.063, -0.976 Trimming A by 20 20 20 45, reference by 20 20 20 45 Drift reached 1.2 A/sec after 5.4 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.04 nm/sec Trimming A by 20 80, reference by 20 75 Tracking image shifted -0.001, -0.004 um; position 0.065, -0.973 Running script # 14 Trimming A by 20 20 60 55, reference by 20 20 60 55 Trimming A by 20 20 60 55, reference by 20 20 60 55 0.4 0.4 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.1 0.1 nm on specimen axes Rate = 1.00161 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_026_-38.0_Oct22_14.34.50.tif Record image shifted 0.000, 0.010 um Tracking reference shifted by 28.5,-3.6 pixels, 0.000,0.010 um Saved Z = 26 at 17.85 min Tilt = -38.00 Mean = 98 Exp = 1.866 X/Y/Z = 0.064 -0.983 4.643 Errors in X/Y Prediction -0.004 0.047 um, 0.3 3.6 % Error in Z Prediction -0.015 Predicted X = 0.067 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.253 StdErr = 0.059 nFit = 5 nDrop = 0 Predicted Z = 3.001 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.017, -0.086 um; position 0.050, 0.339 Drift reached 3.8 A/sec after 5.2 sec, going on Running script # 14 -3.6 2.7 unbinned pixels; 1.3 0.9 nm along two shift axes; -0.8 -1.3 nm on specimen axes Rate = 7.84767 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_027_16.0_Oct22_14.35.27.tif Record image shifted -0.002, -0.015 um Tracking reference shifted by -41.1,10.6 pixels, -0.002,-0.015 um Saved Z = 27 at 18.46 min Tilt = 16.00 Mean = 99 Exp = 1.700 X/Y/Z = 0.055 0.357 3.001 Errors in X/Y Prediction -0.013 0.104 um, 1.0 7.9 % Predicted X = 0.054 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.354 StdErr = 0.067 nFit = 6 nDrop = 0 Predicted Z = 2.916 StdErr = 0.005 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.006, 0.045 um; position 0.060, 0.309 Drift reached 2.7 A/sec after 5.2 sec, going on Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.18 nm/sec Tracking image shifted -0.005, -0.005 um; position 0.066, 0.315 Running script # 14 -1.7 2.3 unbinned pixels; 1.0 0.3 nm along two shift axes; -0.7 -0.7 nm on specimen axes Rate = 4.96845 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_028_18.0_Oct22_14.36.11.tif Record image shifted 0.002, 0.004 um Tracking reference shifted by 10.6,-8.0 pixels, 0.002,0.004 um Saved Z = 28 at 19.19 min Tilt = 18.00 Mean = 99 Exp = 1.726 X/Y/Z = 0.065 0.311 2.921 Errors in X/Y Prediction 0.011 -0.043 um, 0.8 3.3 % Error in Z Prediction 0.005 Predicted X = 0.072 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -1.084 StdErr = 0.041 nFit = 5 nDrop = 0 Predicted Z = 4.659 StdErr = 0.012 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 80, reference by 20 80 Tracking image shifted -0.007, 0.060 um; position 0.078, -1.144 Trimming A by 20 20 20 50, reference by 20 20 20 50 Drift reached 1.2 A/sec after 5.4 sec, going on Running script # 14 Trimming A by 20 20 60 55, reference by 20 20 60 55 Trimming A by 20 20 60 55, reference by 20 20 60 55 0.6 0.9 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 2.01609 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_029_-40.0_Oct22_14.36.46.tif Record image shifted -0.000, 0.007 um Tracking reference shifted by 19.8,-1.7 pixels, -0.000,0.007 um Saved Z = 29 at 19.79 min Tilt = -40.00 Mean = 98 Exp = 1.908 X/Y/Z = 0.079 -1.151 4.659 Errors in X/Y Prediction 0.007 -0.068 um, 0.6 5.2 % Predicted X = 0.083 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -1.297 StdErr = 0.060 nFit = 5 nDrop = 0 Predicted Z = 4.674 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 85, reference by 20 80 Tracking image shifted 0.009, -0.074 um; position 0.074, -1.223 Trimming A by 20 20 45 50, reference by 20 20 45 50 Drift reached 2.2 A/sec after 5.6 sec, going on Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.08 nm/sec Trimming A by 20 85, reference by 20 80 Tracking image shifted -0.001, -0.006 um; position 0.076, -1.218 Running script # 14 Trimming A by 20 20 35 55, reference by 20 20 35 55 Trimming A by 20 20 35 55, reference by 20 20 35 55 0.8 0.3 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.46175 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_030_-42.0_Oct22_14.37.30.tif Record image shifted 0.000, 0.013 um Tracking reference shifted by 35.7,-4.2 pixels, 0.000,0.013 um Saved Z = 30 at 20.52 min Tilt = -42.00 Mean = 97 Exp = 1.953 X/Y/Z = 0.075 -1.231 4.688 Errors in X/Y Prediction -0.008 0.066 um, 0.6 5.1 % Error in Z Prediction 0.014 Predicted X = 0.084 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = -1.361 StdErr = 0.073 nFit = 5 nDrop = 0 Predicted Z = 4.703 StdErr = 0.012 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 90, reference by 20 85 Tracking image shifted 0.016, 0.007 um; position 0.068, -1.369 Trimming A by 20 20 45 40, reference by 20 20 45 40 Drift reached 0.4 A/sec after 5.6 sec, going on Running script # 14 Trimming A by 20 20 30 50, reference by 20 20 30 50 Trimming A by 20 20 30 50, reference by 20 20 30 50 -0.3 0.7 unbinned pixels; 0.3 0.0 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.28584 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_031_-44.0_Oct22_14.38.01.tif Record image shifted 0.000, 0.007 um Tracking reference shifted by 19.4,-2.7 pixels, 0.000,0.007 um Saved Z = 31 at 21.04 min Tilt = -44.00 Mean = 97 Exp = 2.005 X/Y/Z = 0.068 -1.375 4.703 Errors in X/Y Prediction -0.016 -0.014 um, 1.2 1.1 % Predicted X = 0.068 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = 0.311 StdErr = 0.100 nFit = 5 nDrop = 0 Predicted Z = 2.835 StdErr = 0.004 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.017, -0.031 um; position 0.051, 0.342 Drift reached 2.6 A/sec after 5.2 sec, going on Running script # 14 -3.4 3.2 unbinned pixels; 1.5 0.7 nm along two shift axes; -1.0 -1.3 nm on specimen axes Rate = 8.18702 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_032_20.0_Oct22_14.38.38.tif Record image shifted -0.002, -0.011 um Tracking reference shifted by -30.8,8.1 pixels, -0.002,-0.011 um Saved Z = 32 at 21.66 min Tilt = 20.00 Mean = 98 Exp = 1.746 X/Y/Z = 0.055 0.356 2.835 Errors in X/Y Prediction -0.013 0.044 um, 1.0 3.4 % Predicted X = 0.059 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = 0.389 StdErr = 0.098 nFit = 5 nDrop = 0 Predicted Z = 2.750 StdErr = 0.004 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, 0.035 um; position 0.064, 0.354 Drift reached 2.8 A/sec after 5.4 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.17 nm/sec Tracking image shifted -0.005, -0.004 um; position 0.070, 0.359 Running script # 14 -1.4 2.1 unbinned pixels; 0.8 0.2 nm along two shift axes; -0.7 -0.6 nm on specimen axes Rate = 4.39323 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_033_22.0_Oct22_14.39.24.tif Record image shifted 0.003, 0.001 um Tracking reference shifted by 2.8,-7.6 pixels, 0.003,0.001 um Saved Z = 33 at 22.41 min Tilt = 22.00 Mean = 98 Exp = 1.778 X/Y/Z = 0.068 0.358 2.707 Errors in X/Y Prediction 0.009 -0.031 um, 0.7 2.4 % Error in Z Prediction -0.043 Predicted X = 0.065 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = 0.348 StdErr = 0.082 nFit = 5 nDrop = 0 Predicted Z = 2.620 StdErr = 0.030 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, -0.040 um; position 0.066, 0.388 Drift reached 4.4 A/sec after 5.4 sec, going on Running script # 14 -7.7 2.7 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.7 -2.8 nm on specimen axes Rate = 14.31471 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_034_24.0_Oct22_14.39.57.tif Record image shifted -0.002, -0.016 um Tracking reference shifted by -44.5,9.7 pixels, -0.002,-0.016 um Saved Z = 34 at 23.05 min Tilt = 24.00 Mean = 98 Exp = 1.809 X/Y/Z = 0.070 0.409 2.620 Errors in X/Y Prediction 0.005 0.061 um, 0.3 4.7 % Predicted X = 0.063 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -1.518 StdErr = 0.073 nFit = 5 nDrop = 0 Predicted Z = 4.721 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 70, reference by 20 85 Tracking image shifted -0.017, -0.025 um; position 0.080, -1.493 Trimming A by 20 20 25 30, reference by 20 20 25 30 Drift reached 1.7 A/sec after 5.6 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.09 nm/sec Trimming A by 20 75, reference by 20 85 Tracking image shifted -0.002, 0.004 um; position 0.082, -1.498 Running script # 14 Trimming A by 20 20 25 50, reference by 20 20 25 50 Trimming A by 20 20 25 50, reference by 20 20 25 50 1.1 0.5 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.11306 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_035_-46.0_Oct22_14.40.51.tif Record image shifted 0.000, 0.014 um Tracking reference shifted by 40.9,-4.4 pixels, 0.000,0.014 um Saved Z = 35 at 23.88 min Tilt = -46.00 Mean = 97 Exp = 2.072 X/Y/Z = 0.082 -1.513 4.719 Errors in X/Y Prediction 0.019 0.005 um, 1.5 0.4 % Error in Z Prediction -0.002 Predicted X = 0.082 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = -1.638 StdErr = 0.062 nFit = 5 nDrop = 0 Predicted Z = 4.740 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Trimming A by 20 60, reference by 20 60 Tracking image shifted 0.007, 0.015 um; position 0.075, -1.653 Drift reached 0.3 A/sec after 8.0 sec, going on Running script # 14 Trimming A by 20 20 20 40, reference by 20 20 20 40 Trimming A by 20 20 20 40, reference by 20 20 20 40 0.0 0.7 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.3 -0.0 nm on specimen axes Rate = 1.25881 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_036_-48.0_Oct22_14.41.26.tif Record image shifted -0.000, 0.008 um Tracking reference shifted by 22.5,-1.6 pixels, -0.000,0.008 um Saved Z = 36 at 24.45 min Tilt = -48.00 Mean = 97 Exp = 2.143 X/Y/Z = 0.076 -1.661 4.740 Errors in X/Y Prediction -0.006 -0.023 um, 0.5 1.8 % Predicted X = 0.084 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = -1.841 StdErr = 0.034 nFit = 5 nDrop = 0 Predicted Z = 4.760 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 85, reference by 20 60 Tracking image shifted 0.008, -0.085 um; position 0.075, -1.755 Drift reached 1.9 A/sec after 5.8 sec, going on Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.09 nm/sec Trimming A by 20 55, reference by 20 60 Tracking image shifted -0.001, 0.003 um; position 0.077, -1.759 Running script # 14 Trimming A by 20 20 20 25, reference by 20 20 20 25 Trimming A by 20 20 20 30, reference by 20 20 20 25 0.5 0.3 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 1.07665 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_037_-50.0_Oct22_14.42.11.tif Record image shifted 0.000, 0.013 um Tracking reference shifted by 37.9,-3.6 pixels, 0.000,0.013 um Saved Z = 37 at 25.20 min Tilt = -50.00 Mean = 96 Exp = 2.218 X/Y/Z = 0.077 -1.773 4.687 Errors in X/Y Prediction -0.007 0.068 um, 0.5 5.2 % Error in Z Prediction -0.072 Predicted X = 0.064 StdErr = 0.018 nFit = 4 nDrop = 0 Predicted Y = -1.894 StdErr = 0.019 nFit = 5 nDrop = 1 Predicted Z = 4.698 StdErr = 0.040 nFit = 3 nDrop = 0 Need regular tracking - Trimming A by 20 35, reference by 20 40 Tracking image shifted -0.006, -0.015 um; position 0.070, -1.879 Drift reached 0.3 A/sec after 6.0 sec, going on Running script # 14 Trimming A by 20 20 20 25, reference by 20 20 20 25 Trimming A by 20 20 20 25, reference by 20 20 20 25 0.6 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.81561 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_038_-52.0_Oct22_14.42.43.tif Record image shifted -0.002, 0.008 um Tracking reference shifted by 23.2,3.1 pixels, -0.002,0.008 um Saved Z = 38 at 25.75 min Tilt = -52.00 Mean = 95 Exp = 2.308 X/Y/Z = 0.072 -1.888 4.698 Errors in X/Y Prediction 0.008 0.007 um, 0.6 0.5 % Predicted X = 0.079 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = 0.479 StdErr = 0.049 nFit = 5 nDrop = 1 Predicted Z = 2.525 StdErr = 0.035 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.026, 0.018 um; position 0.105, 0.461 Drift reached 4.3 A/sec after 5.4 sec, going on Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.35 nm/sec Tracking image shifted -0.002, -0.010 um; position 0.107, 0.473 Running script # 14 -5.0 1.4 unbinned pixels; 1.0 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 9.11048 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_039_26.0_Oct22_14.43.35.tif Record image shifted 0.004, 0.002 um Tracking reference shifted by 3.5,-10.9 pixels, 0.004,0.002 um Saved Z = 39 at 26.59 min Tilt = 26.00 Mean = 98 Exp = 1.847 X/Y/Z = 0.104 0.474 2.455 Errors in X/Y Prediction 0.025 -0.005 um, 1.9 0.4 % Error in Z Prediction -0.070 Predicted X = 0.117 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Y = 0.540 StdErr = 0.039 nFit = 5 nDrop = 0 Predicted Z = 2.370 StdErr = 0.043 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.023, 0.174 um; position 0.093, 0.365 Drift reached 3.8 A/sec after 5.3 sec, going on Running script # 14 -1.6 5.2 unbinned pixels; 1.9 0.1 nm along two shift axes; -1.8 -0.7 nm on specimen axes Rate = 9.58149 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_040_28.0_Oct22_14.44.08.tif Record image shifted -0.000, -0.008 um Tracking reference shifted by -21.3,2.7 pixels, -0.000,-0.008 um Saved Z = 40 at 27.16 min Tilt = 28.00 Mean = 97 Exp = 1.880 X/Y/Z = 0.098 0.375 2.370 Errors in X/Y Prediction -0.019 -0.165 um, 1.5 12.6 % Predicted X = 0.105 StdErr = 0.048 nFit = 4 nDrop = 0 Predicted Y = 0.346 StdErr = 0.116 nFit = 5 nDrop = 0 Predicted Z = 2.265 StdErr = 0.048 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, -0.020 um; position 0.091, 0.365 Drift reached 3.7 A/sec after 5.4 sec, going on Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.27 nm/sec Tracking image shifted -0.003, -0.010 um; position 0.094, 0.378 Running script # 14 -4.8 1.3 unbinned pixels; 1.0 1.4 nm along two shift axes; -0.3 -1.7 nm on specimen axes Rate = 8.73176 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_041_30.0_Oct22_14.44.53.tif Record image shifted 0.000, -0.008 um Tracking reference shifted by -23.2,1.9 pixels, 0.000,-0.008 um Saved Z = 41 at 27.91 min Tilt = 30.00 Mean = 98 Exp = 1.928 X/Y/Z = 0.095 0.388 2.264 Errors in X/Y Prediction -0.010 0.042 um, 0.8 3.2 % Error in Z Prediction -0.001 Predicted X = 0.062 StdErr = 0.029 nFit = 4 nDrop = 0 Predicted Y = 0.288 StdErr = 0.105 nFit = 5 nDrop = 0 Predicted Z = 2.143 StdErr = 0.040 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.026, -0.035 um; position 0.088, 0.324 Drift reached 3.0 A/sec after 5.6 sec, going on Running script # 14 -1.5 4.4 unbinned pixels; 1.7 0.0 nm along two shift axes; -1.5 -0.7 nm on specimen axes Rate = 8.26847 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_042_32.0_Oct22_14.45.27.tif Record image shifted -0.003, -0.008 um Tracking reference shifted by -21.8,11.7 pixels, -0.003,-0.008 um Saved Z = 42 at 28.47 min Tilt = 32.00 Mean = 97 Exp = 1.977 X/Y/Z = 0.095 0.333 2.143 Errors in X/Y Prediction 0.032 0.044 um, 2.5 3.4 % Predicted X = 0.071 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -1.987 StdErr = 0.017 nFit = 5 nDrop = 0 Predicted Z = 4.697 StdErr = 0.047 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 20, reference by 20 40 Tracking image shifted -0.027, 0.109 um; position 0.098, -2.096 Drift reached 2.0 A/sec after 6.0 sec, going on Measured defocus = -4.30 microns changed by -0.20 to target drift = 0.11 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.05 nm/sec Trimming A by 20 60, reference by 20 40 Tracking image shifted 0.001, 0.008 um; position 0.097, -2.105 Running script # 14 Trimming A by 20 20 20 30, reference by 20 20 20 30 Trimming A by 20 20 20 30, reference by 20 20 20 30 0.2 2.7 unbinned pixels; 0.9 0.4 nm along two shift axes; -1.0 -0.0 nm on specimen axes Rate = 4.80692 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_043_-54.0_Oct22_14.46.29.tif Record image shifted 0.001, -0.092 um Tracking reference shifted by -259.9,19.6 pixels, 0.001,-0.092 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 43 at 29.59 min Tilt = -54.00 Mean = 94 Exp = 2.422 X/Y/Z = 0.097 -2.013 4.481 Errors in X/Y Prediction 0.026 -0.026 um, 2.0 2.0 % Error in Z Prediction -0.216 Predicted X = 0.102 StdErr = 0.022 nFit = 3 nDrop = 0 Predicted Y = -2.117 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 4.451 StdErr = 0.112 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.089 um; position 0.097, -2.206 Drift reached 2.3 A/sec after 6.2 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.09 nm/sec Trimming A by 20 30, reference by 20 20 Tracking image shifted 0.001, 0.005 um; position 0.098, -2.211 Running script # 14 0.5 0.9 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.72501 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_044_-56.0_Oct22_14.47.25.tif Record image shifted -0.006, -0.030 um Tracking reference shifted by -83.6,24.2 pixels, -0.006,-0.030 um Saved Z = 44 at 30.45 min Tilt = -56.00 Mean = 95 Exp = 2.577 X/Y/Z = 0.104 -2.181 4.407 Errors in X/Y Prediction 0.002 -0.064 um, 0.1 4.9 % Error in Z Prediction -0.044 Predicted X = 0.129 StdErr = 0.031 nFit = 4 nDrop = 0 Predicted Y = -2.348 StdErr = 0.022 nFit = 5 nDrop = 1 Predicted Z = 4.358 StdErr = 0.076 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 50, reference by 20 20 Tracking image shifted 0.028, -0.043 um; position 0.100, -2.305 Drift reached 1.1 A/sec after 6.4 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.05 nm/sec Tracking image shifted -0.002, -0.015 um; position 0.103, -2.291 Running script # 14 0.2 1.1 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.4 0.0 nm on specimen axes Rate = 1.91325 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_045_-58.0_Oct22_14.48.13.tif Record image shifted -0.003, -0.024 um Tracking reference shifted by -67.9,14.3 pixels, -0.003,-0.024 um Saved Z = 45 at 31.25 min Tilt = -58.00 Mean = 94 Exp = 2.708 X/Y/Z = 0.106 -2.266 4.384 Errors in X/Y Prediction -0.022 0.082 um, 1.7 6.2 % Error in Z Prediction 0.025 Predicted X = 0.112 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -2.400 StdErr = 0.058 nFit = 5 nDrop = 0 Predicted Z = 4.307 StdErr = 0.060 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.011, -0.038 um; position 0.101, -2.362 Drift reached 0.9 A/sec after 6.6 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.05 nm/sec Tracking image shifted -0.001, -0.006 um; position 0.103, -2.356 Running script # 14 0.1 0.8 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.3 0.0 nm on specimen axes Rate = 1.36344 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_046_-60.0_Oct22_14.49.03.tif Record image shifted -0.003, -0.025 um Tracking reference shifted by -69.6,13.6 pixels, -0.003,-0.025 um Saved Z = 46 at 32.09 min Tilt = -60.00 Mean = 93 Exp = 2.877 X/Y/Z = 0.106 -2.331 4.340 Errors in X/Y Prediction -0.006 0.069 um, 0.5 5.3 % Error in Z Prediction 0.032 Predicted X = 0.107 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -2.377 StdErr = 0.050 nFit = 5 nDrop = 0 Predicted Z = 4.291 StdErr = 0.025 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Trimming A by 25 45, reference by 20 20 Tracking image shifted 0.004, 0.121 um; position 0.103, -2.498 Drift reached 1.2 A/sec after 6.8 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.05 nm/sec Tracking image shifted 0.005, -0.031 um; position 0.100, -2.467 Running script # 14 Trimming A by 21 25 20 20, reference by 21 25 20 20 Trimming A by 21 25 20 20, reference by 21 25 20 20 1.8 1.3 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.5 0.6 nm on specimen axes Rate = 3.87501 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_047_-62.0_Oct22_14.49.53.tif Record image shifted -0.005, -0.026 um Tracking reference shifted by -73.5,19.6 pixels, -0.005,-0.026 um Saved Z = 47 at 32.92 min Tilt = -62.00 Mean = 92 Exp = 3.097 X/Y/Z = 0.105 -2.441 4.298 Errors in X/Y Prediction -0.002 -0.065 um, 0.2 5.0 % Error in Z Prediction 0.007 Predicted X = 0.093 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.238 StdErr = 0.077 nFit = 6 nDrop = 0 Predicted Z = 2.032 StdErr = 0.046 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.041, -0.166 um; position 0.134, 0.404 Drift reached 5.6 A/sec after 5.5 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.34 nm/sec Tracking image shifted -0.004, -0.011 um; position 0.139, 0.418 Running script # 14 -5.5 2.0 unbinned pixels; 1.3 1.6 nm along two shift axes; -0.5 -2.0 nm on specimen axes Rate = 10.19562 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_048_34.0_Oct22_14.50.47.tif Record image shifted 0.004, -0.004 um Tracking reference shifted by -11.3,-11.3 pixels, 0.004,-0.004 um Saved Z = 48 at 33.80 min Tilt = 34.00 Mean = 97 Exp = 2.037 X/Y/Z = 0.135 0.424 1.973 Errors in X/Y Prediction 0.042 0.186 um, 3.2 14.2 % Error in Z Prediction -0.059 Predicted X = 0.148 StdErr = 0.030 nFit = 3 nDrop = 0 Predicted Y = 0.512 StdErr = 0.081 nFit = 5 nDrop = 0 Predicted Z = 1.871 StdErr = 0.042 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 25 30, reference by 20 20 Tracking image shifted 0.034, 0.231 um; position 0.114, 0.280 Drift reached 3.1 A/sec after 5.5 sec, going on Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.14 nm/sec Tracking image shifted -0.001, -0.006 um; position 0.117, 0.287 Running script # 14 -1.2 1.9 unbinned pixels; 0.8 0.2 nm along two shift axes; -0.6 -0.5 nm on specimen axes Rate = 3.94222 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_049_36.0_Oct22_14.51.33.tif Record image shifted -0.000, -0.006 um Tracking reference shifted by -16.5,1.8 pixels, -0.000,-0.006 um Saved Z = 49 at 34.58 min Tilt = 36.00 Mean = 96 Exp = 2.098 X/Y/Z = 0.118 0.293 1.932 Errors in X/Y Prediction -0.031 -0.218 um, 2.4 16.7 % Error in Z Prediction 0.061 Predicted X = 0.138 StdErr = 0.043 nFit = 3 nDrop = 0 Predicted Y = 0.254 StdErr = 0.141 nFit = 5 nDrop = 0 Predicted Z = 1.796 StdErr = 0.057 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.018, -0.043 um; position 0.121, 0.297 Drift reached 3.6 A/sec after 5.8 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.27 nm/sec Tracking image shifted -0.003, -0.012 um; position 0.124, 0.312 Running script # 14 -5.1 1.1 unbinned pixels; 1.0 1.6 nm along two shift axes; -0.2 -1.8 nm on specimen axes Rate = 9.13276 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_050_38.0_Oct22_14.52.20.tif Record image shifted 0.000, -0.009 um Tracking reference shifted by -25.3,1.5 pixels, 0.000,-0.009 um Saved Z = 50 at 35.36 min Tilt = 38.00 Mean = 96 Exp = 2.175 X/Y/Z = 0.124 0.323 1.790 Errors in X/Y Prediction -0.015 0.069 um, 1.1 5.3 % Error in Z Prediction -0.005 Predicted X = 0.114 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = 0.275 StdErr = 0.150 nFit = 5 nDrop = 0 Predicted Z = 1.674 StdErr = 0.058 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.004, 0.023 um; position 0.118, 0.252 Drift reached 2.0 A/sec after 5.8 sec, going on Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.15 nm/sec Tracking image shifted -0.004, -0.005 um; position 0.124, 0.258 Running script # 14 -1.5 1.5 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.5 -0.6 nm on specimen axes Rate = 3.66902 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_051_40.0_Oct22_14.53.07.tif Record image shifted -0.000, -0.005 um Tracking reference shifted by -15.4,2.1 pixels, -0.000,-0.005 um Saved Z = 51 at 36.15 min Tilt = 40.00 Mean = 96 Exp = 2.260 X/Y/Z = 0.124 0.264 1.713 Errors in X/Y Prediction 0.010 -0.011 um, 0.8 0.8 % Error in Z Prediction 0.039 Predicted X = 0.129 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.186 StdErr = 0.144 nFit = 5 nDrop = 0 Predicted Z = 1.585 StdErr = 0.049 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.076 um; position 0.124, 0.262 Drift reached 3.9 A/sec after 6.1 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.26 nm/sec Tracking image shifted -0.002, -0.013 um; position 0.127, 0.277 Running script # 14 -4.9 1.3 unbinned pixels; 1.0 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 8.91811 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo4_L1G1_052_42.0_Oct22_14.53.56.tif Record image shifted 0.000, -0.010 um Tracking reference shifted by -28.2,1.4 pixels, 0.000,-0.010 um Saved Z = 52 at 36.97 min Tilt = 42.00 Mean = 95 Exp = 2.355 X/Y/Z = 0.127 0.288 1.566 Errors in X/Y Prediction -0.002 0.102 um, 0.1 7.8 % Error in Z Prediction -0.020 Predicted X = 0.101 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = -2.486 StdErr = 0.048 nFit = 6 nDrop = 0 Predicted Z = 4.265 StdErr = 0.012 nFit = 4 nDrop = 1 Need regular tracking - Trimming A by 30 50, reference by 20 20 Tracking image shifted -0.011, 0.026 um; position 0.112, -2.512 Drift reached 1.6 A/sec after 7.2 sec, going on Running script # 14 Trimming A by 20 35 20 50, reference by 20 35 20 50 2020/10/22 14:54 D:\Users\Ben\201022_Jans_cells\SEM\tomo4_L1G1.st 0 53 3838 3710 1509 -10.0 42.0 2.00 REG 37.0 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Measured defocus = -4.31 microns changed by 0.81 to target drift = 0.10 nm/sec Need regular tracking - Need autofocus Tracking image shifted -0.000, -0.000 um; position 0.000, 0.000 Drift reached 0.1 A/sec after 5.2 sec, going on Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.01 nm/sec Tracking image shifted -0.000, -0.000 um; position 0.000, 0.000 Running script # 14 -0.2 0.3 unbinned pixels; 0.1 0.0 nm along two shift axes; -0.1 -0.1 nm on specimen axes Rate = 0.64745 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_000_-10.0_Oct22_15.00.47.tif Record image shifted -0.007, -0.004 um Tracking reference shifted by -10.6,20.3 pixels, -0.007,-0.004 um Saved Z = 0 at -0.00 min Tilt = -10.00 Mean = 145 Exp = 1.560 X/Y/Z = 0.007 0.005 4.050 Need regular tracking - Need autofocus Tracking image shifted -0.021, -0.149 um; position 0.029, 0.153 Drift reached 2.0 A/sec after 5.3 sec, going on Measured defocus = -3.57 microns changed by 0.07 to target drift = 0.06 nm/sec Tracking image shifted -0.003, 0.001 um; position 0.032, 0.152 Running script # 14 0.4 0.6 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.30483 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_001_-12.0_Oct22_15.01.27.tif Record image shifted -0.001, -0.001 um Tracking reference shifted by -2.5,1.8 pixels, -0.001,-0.001 um Saved Z = 1 at 0.68 min Tilt = -12.00 Mean = 140 Exp = 1.501 X/Y/Z = 0.033 0.153 4.116 Predicted X = 0.059 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.301 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 4.183 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.023, 0.164 um; position 0.036, 0.137 Drift reached 1.5 A/sec after 5.1 sec, going on Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.06 nm/sec Tracking image shifted 0.003, -0.001 um; position 0.034, 0.139 Running script # 14 -0.4 0.5 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.10965 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_002_-14.0_Oct22_15.02.09.tif Record image shifted 0.000, -0.002 um Tracking reference shifted by -4.9,-0.3 pixels, 0.000,-0.002 um Saved Z = 2 at 1.36 min Tilt = -14.00 Mean = 140 Exp = 1.501 X/Y/Z = 0.034 0.140 4.185 Errors in X/Y Prediction -0.026 -0.161 um, 2.0 12.3 % Error in Z Prediction 0.003 Need regular tracking - Need autofocus Tracking image shifted -0.040, -0.050 um; position 0.048, 0.055 Drift reached 4.2 A/sec after 5.2 sec, going on Measured defocus = -3.36 microns changed by -0.14 to target drift = 0.16 nm/sec Tracking image shifted -0.001, 0.009 um; position 0.049, 0.044 Running script # 14 2.0 0.1 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.1 0.7 nm on specimen axes Rate = 3.56661 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_003_-8.0_Oct22_15.02.53.tif Record image shifted 0.000, 0.002 um Tracking reference shifted by 4.5,-1.2 pixels, 0.000,0.002 um Saved Z = 3 at 2.09 min Tilt = -8.00 Mean = 140 Exp = 1.501 X/Y/Z = 0.049 0.042 3.912 Need regular tracking - Need autofocus Tracking image shifted 0.004, 0.041 um; position 0.045, 0.001 Drift reached 0.5 A/sec after 5.2 sec, going on Measured defocus = -3.40 microns changed by -0.10 to target drift = 0.05 nm/sec Tracking image shifted 0.001, -0.003 um; position 0.045, 0.003 Running script # 14 -0.7 0.3 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.1 -0.3 nm on specimen axes Rate = 1.36636 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_004_-6.0_Oct22_15.03.36.tif Record image shifted 0.000, -0.001 um Tracking reference shifted by -4.0,0.3 pixels, 0.000,-0.001 um Saved Z = 4 at 2.89 min Tilt = -6.00 Mean = 140 Exp = 1.496 X/Y/Z = 0.045 0.005 3.809 Predicted X = 0.051 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = 0.235 StdErr = 0.120 nFit = 3 nDrop = 0 Predicted Z = 4.253 StdErr = 0.002 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.012, 0.186 um; position 0.063, 0.049 Drift reached 2.2 A/sec after 5.2 sec, going on Running script # 14 -1.8 1.0 unbinned pixels; 0.5 0.5 nm along two shift axes; -0.3 -0.7 nm on specimen axes Rate = 3.60407 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_005_-16.0_Oct22_15.04.11.tif Record image shifted 0.003, -0.004 um Tracking reference shifted by -12.7,-8.5 pixels, 0.003,-0.004 um Saved Z = 5 at 3.41 min Tilt = -16.00 Mean = 140 Exp = 1.505 X/Y/Z = 0.061 0.054 4.253 Errors in X/Y Prediction 0.010 -0.181 um, 0.8 13.8 % Predicted X = 0.070 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.018 StdErr = 0.055 nFit = 3 nDrop = 0 Predicted Z = 4.321 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.015, -0.030 um; position 0.056, 0.048 Drift reached 1.7 A/sec after 5.2 sec, going on Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.07 nm/sec Tracking image shifted -0.000, 0.002 um; position 0.056, 0.044 Running script # 14 0.6 0.2 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 1.19007 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_006_-18.0_Oct22_15.04.53.tif Record image shifted -0.003, 0.005 um Tracking reference shifted by 14.6,8.0 pixels, -0.003,0.005 um Saved Z = 6 at 4.10 min Tilt = -18.00 Mean = 140 Exp = 1.507 X/Y/Z = 0.059 0.039 4.294 Errors in X/Y Prediction -0.011 0.022 um, 0.9 1.7 % Error in Z Prediction -0.026 Predicted X = 0.041 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.032 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.705 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.014, -0.131 um; position 0.055, 0.099 Drift reached 3.6 A/sec after 5.2 sec, going on Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.14 nm/sec Tracking image shifted -0.002, 0.008 um; position 0.058, 0.089 Running script # 14 1.6 0.5 unbinned pixels; 0.0 0.6 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.86017 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_007_-4.0_Oct22_15.05.38.tif Record image shifted 0.000, 0.002 um Tracking reference shifted by 5.3,-1.2 pixels, 0.000,0.002 um Saved Z = 7 at 4.85 min Tilt = -4.00 Mean = 140 Exp = 1.497 X/Y/Z = 0.058 0.087 3.700 Errors in X/Y Prediction 0.017 0.119 um, 1.3 9.1 % Error in Z Prediction -0.006 Predicted X = 0.059 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = 0.089 StdErr = 0.088 nFit = 3 nDrop = 0 Predicted Z = 3.595 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, -0.049 um; position 0.052, 0.138 Drift reached 1.3 A/sec after 5.2 sec, going on Running script # 14 -2.2 0.9 unbinned pixels; 0.5 0.6 nm along two shift axes; -0.2 -0.8 nm on specimen axes Rate = 4.08764 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_008_-2.0_Oct22_15.06.10.tif Record image shifted -0.001, -0.006 um Tracking reference shifted by -15.7,3.9 pixels, -0.001,-0.006 um Saved Z = 8 at 5.38 min Tilt = -2.00 Mean = 139 Exp = 1.492 X/Y/Z = 0.053 0.145 3.595 Errors in X/Y Prediction -0.006 0.056 um, 0.5 4.3 % Predicted X = 0.077 StdErr = 0.022 nFit = 3 nDrop = 0 Predicted Y = -0.023 StdErr = 0.053 nFit = 3 nDrop = 0 Predicted Z = 4.355 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.051 um; position 0.072, -0.074 Drift reached 1.3 A/sec after 5.2 sec, going on Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.06 nm/sec Tracking image shifted 0.001, -0.002 um; position 0.072, -0.072 Running script # 14 -0.4 0.4 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.1 -0.2 nm on specimen axes Rate = 1.00575 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_009_-20.0_Oct22_15.06.52.tif Record image shifted 0.000, -0.002 um Tracking reference shifted by -6.3,0.2 pixels, 0.000,-0.002 um Saved Z = 9 at 6.09 min Tilt = -20.00 Mean = 139 Exp = 1.512 X/Y/Z = 0.072 -0.070 4.349 Errors in X/Y Prediction -0.005 -0.046 um, 0.4 3.5 % Error in Z Prediction -0.006 Predicted X = 0.066 StdErr = 0.029 nFit = 4 nDrop = 0 Predicted Y = -0.148 StdErr = 0.109 nFit = 4 nDrop = 0 Predicted Z = 4.403 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, -0.089 um; position 0.067, -0.060 Drift reached 2.4 A/sec after 5.2 sec, going on Running script # 14 1.9 1.0 unbinned pixels; 0.1 0.8 nm along two shift axes; -0.4 0.6 nm on specimen axes Rate = 3.80913 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_010_-22.0_Oct22_15.07.22.tif Record image shifted -0.000, 0.001 um Tracking reference shifted by 3.9,0.9 pixels, -0.000,0.001 um Saved Z = 10 at 6.57 min Tilt = -22.00 Mean = 139 Exp = 1.522 X/Y/Z = 0.069 -0.063 4.403 Errors in X/Y Prediction 0.002 0.086 um, 0.2 6.6 % Predicted X = 0.060 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = 0.219 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Z = 3.489 StdErr = 0.006 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.008, -0.010 um; position 0.068, 0.229 Drift reached 3.4 A/sec after 5.3 sec, going on Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.15 nm/sec Tracking image shifted -0.001, 0.007 um; position 0.069, 0.220 Running script # 14 1.6 0.7 unbinned pixels; 0.0 0.6 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 3.08663 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_011_-0.0_Oct22_15.08.07.tif Record image shifted 0.001, 0.004 um Tracking reference shifted by 11.5,-3.6 pixels, 0.001,0.004 um Saved Z = 11 at 7.33 min Tilt = -0.00 Mean = 141 Exp = 1.499 X/Y/Z = 0.069 0.215 3.468 Errors in X/Y Prediction 0.008 -0.004 um, 0.6 0.3 % Error in Z Prediction -0.021 Predicted X = 0.071 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = 0.277 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Z = 3.355 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.006, 0.008 um; position 0.065, 0.269 Drift reached 2.7 A/sec after 5.2 sec, going on Running script # 14 -5.1 1.2 unbinned pixels; 1.0 1.6 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 9.26084 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_012_2.0_Oct22_15.08.39.tif Record image shifted -0.001, -0.007 um Tracking reference shifted by -18.3,3.5 pixels, -0.001,-0.007 um Saved Z = 12 at 7.86 min Tilt = 2.00 Mean = 139 Exp = 1.492 X/Y/Z = 0.066 0.279 3.355 Errors in X/Y Prediction -0.005 0.002 um, 0.4 0.1 % Predicted X = 0.073 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Y = -0.093 StdErr = 0.078 nFit = 5 nDrop = 0 Predicted Z = 4.458 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.011, 0.058 um; position 0.083, -0.151 Drift reached 0.6 A/sec after 5.2 sec, going on Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.03 nm/sec Tracking image shifted 0.000, -0.002 um; position 0.084, -0.149 Running script # 14 -0.3 0.8 unbinned pixels; 0.3 0.0 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.41784 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_013_-24.0_Oct22_15.09.22.tif Record image shifted 0.001, -0.001 um Tracking reference shifted by -3.3,-1.5 pixels, 0.001,-0.001 um Saved Z = 13 at 8.58 min Tilt = -24.00 Mean = 139 Exp = 1.528 X/Y/Z = 0.083 -0.148 4.400 Errors in X/Y Prediction 0.011 -0.055 um, 0.8 4.2 % Error in Z Prediction -0.058 Predicted X = 0.086 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.202 StdErr = 0.083 nFit = 5 nDrop = 0 Predicted Z = 4.438 StdErr = 0.026 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.019 um; position 0.080, -0.184 Drift reached 1.2 A/sec after 5.2 sec, going on Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.081, -0.187 Running script # 14 0.9 0.5 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.7456 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_014_-26.0_Oct22_15.10.02.tif Record image shifted 0.000, 0.000 um Tracking reference shifted by 0.9,-1.0 pixels, 0.000,0.000 um Saved Z = 14 at 9.25 min Tilt = -26.00 Mean = 139 Exp = 1.535 X/Y/Z = 0.081 -0.188 4.420 Errors in X/Y Prediction -0.005 0.014 um, 0.4 1.1 % Error in Z Prediction -0.018 Predicted X = 0.074 StdErr = 0.025 nFit = 4 nDrop = 0 Predicted Y = 0.350 StdErr = 0.012 nFit = 4 nDrop = 0 Predicted Z = 3.241 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.010, -0.005 um; position 0.084, 0.355 Drift reached 2.4 A/sec after 5.2 sec, going on Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.09 nm/sec Tracking image shifted -0.002, 0.005 um; position 0.086, 0.349 Running script # 14 0.6 0.7 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.70806 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_015_4.0_Oct22_15.10.48.tif Record image shifted 0.001, 0.005 um Tracking reference shifted by 13.6,-3.1 pixels, 0.001,0.005 um Saved Z = 15 at 10.01 min Tilt = 4.00 Mean = 140 Exp = 1.498 X/Y/Z = 0.085 0.344 3.265 Errors in X/Y Prediction 0.011 -0.007 um, 0.9 0.5 % Error in Z Prediction 0.024 Predicted X = 0.097 StdErr = 0.026 nFit = 4 nDrop = 0 Predicted Y = 0.412 StdErr = 0.008 nFit = 5 nDrop = 0 Predicted Z = 3.151 StdErr = 0.019 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.017, 0.034 um; position 0.080, 0.378 Drift reached 7.4 A/sec after 5.2 sec, going on Running script # 14 -12.2 4.4 unbinned pixels; 2.9 3.5 nm along two shift axes; -1.2 -4.4 nm on specimen axes Rate = 22.73778 A/sec -8.7 3.0 unbinned pixels; 2.0 2.5 nm along two shift axes; -0.8 -3.2 nm on specimen axes Rate = 16.16605 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_016_6.0_Oct22_15.11.25.tif Record image shifted -0.000, -0.012 um Tracking reference shifted by -33.2,3.2 pixels, -0.000,-0.012 um Saved Z = 16 at 10.63 min Tilt = 6.00 Mean = 139 Exp = 1.498 X/Y/Z = 0.083 0.401 3.151 Errors in X/Y Prediction -0.014 -0.011 um, 1.1 0.8 % Predicted X = 0.090 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.223 StdErr = 0.078 nFit = 5 nDrop = 0 Predicted Z = 4.457 StdErr = 0.020 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, 0.080 um; position 0.094, -0.303 Drift reached 1.6 A/sec after 5.2 sec, going on Running script # 14 0.2 1.5 unbinned pixels; 0.5 0.2 nm along two shift axes; -0.5 0.0 nm on specimen axes Rate = 2.63612 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_017_-28.0_Oct22_15.11.58.tif Record image shifted 0.002, -0.002 um Tracking reference shifted by -6.7,-4.1 pixels, 0.002,-0.002 um Saved Z = 17 at 11.18 min Tilt = -28.00 Mean = 139 Exp = 1.543 X/Y/Z = 0.094 -0.301 4.457 Errors in X/Y Prediction 0.004 -0.078 um, 0.3 6.0 % Predicted X = 0.096 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -0.428 StdErr = 0.052 nFit = 5 nDrop = 0 Predicted Z = 4.487 StdErr = 0.023 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, -0.056 um; position 0.089, -0.372 Drift reached 1.6 A/sec after 5.1 sec, going on Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.002 um; position 0.091, -0.374 Running script # 14 0.7 0.6 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.59741 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_018_-30.0_Oct22_15.12.38.tif Record image shifted -0.001, 0.003 um Tracking reference shifted by 8.4,3.1 pixels, -0.001,0.003 um Saved Z = 18 at 11.85 min Tilt = -30.00 Mean = 139 Exp = 1.553 X/Y/Z = 0.092 -0.377 4.473 Errors in X/Y Prediction -0.004 0.050 um, 0.3 3.9 % Error in Z Prediction -0.014 Predicted X = 0.091 StdErr = 0.029 nFit = 4 nDrop = 0 Predicted Y = 0.457 StdErr = 0.003 nFit = 5 nDrop = 1 Predicted Z = 3.043 StdErr = 0.022 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.012, 0.003 um; position 0.103, 0.454 Drift reached 0.5 A/sec after 5.2 sec, going on Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.001 um; position 0.104, 0.453 Running script # 14 -0.2 1.2 unbinned pixels; 0.4 0.1 nm along two shift axes; -0.4 -0.1 nm on specimen axes Rate = 2.19992 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_019_8.0_Oct22_15.13.25.tif Record image shifted 0.000, 0.010 um Tracking reference shifted by 28.0,-3.4 pixels, 0.000,0.010 um Saved Z = 19 at 12.63 min Tilt = 8.00 Mean = 140 Exp = 1.505 X/Y/Z = 0.105 0.443 3.067 Errors in X/Y Prediction 0.014 -0.014 um, 1.1 1.1 % Error in Z Prediction 0.024 Predicted X = 0.110 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.488 StdErr = 0.007 nFit = 6 nDrop = 0 Predicted Z = 2.966 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.017, 0.074 um; position 0.093, 0.414 Drift reached 3.6 A/sec after 5.2 sec, going on Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.24 nm/sec Tracking image shifted -0.000, -0.008 um; position 0.094, 0.424 Running script # 14 -4.4 1.4 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.04746 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_020_10.0_Oct22_15.14.08.tif Record image shifted -0.001, -0.002 um Tracking reference shifted by -5.8,2.3 pixels, -0.001,-0.002 um Saved Z = 20 at 13.35 min Tilt = 10.00 Mean = 139 Exp = 1.505 X/Y/Z = 0.095 0.428 2.986 Errors in X/Y Prediction -0.015 -0.061 um, 1.1 4.6 % Error in Z Prediction 0.020 Predicted X = 0.100 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -0.508 StdErr = 0.045 nFit = 6 nDrop = 0 Predicted Z = 4.496 StdErr = 0.004 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.012, -0.032 um; position 0.113, -0.476 Drift reached 0.9 A/sec after 5.3 sec, going on Running script # 14 0.2 1.3 unbinned pixels; 0.4 0.2 nm along two shift axes; -0.5 0.0 nm on specimen axes Rate = 2.31214 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_021_-32.0_Oct22_15.14.42.tif Record image shifted -0.001, -0.003 um Tracking reference shifted by -7.3,3.2 pixels, -0.001,-0.003 um Saved Z = 21 at 13.91 min Tilt = -32.00 Mean = 139 Exp = 1.566 X/Y/Z = 0.114 -0.474 4.496 Errors in X/Y Prediction 0.014 0.034 um, 1.1 2.6 % Predicted X = 0.121 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = -0.588 StdErr = 0.044 nFit = 5 nDrop = 0 Predicted Z = 4.521 StdErr = 0.004 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, -0.022 um; position 0.113, -0.565 Drift reached 1.4 A/sec after 5.2 sec, going on Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.06 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.115, -0.569 Running script # 14 0.9 0.5 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.81735 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_022_-34.0_Oct22_15.15.28.tif Record image shifted 0.002, 0.003 um Tracking reference shifted by 8.5,-5.5 pixels, 0.002,0.003 um Saved Z = 22 at 14.67 min Tilt = -34.00 Mean = 139 Exp = 1.580 X/Y/Z = 0.113 -0.573 4.533 Errors in X/Y Prediction -0.008 0.015 um, 0.6 1.2 % Error in Z Prediction 0.012 Predicted X = 0.097 StdErr = 0.037 nFit = 4 nDrop = 0 Predicted Y = 0.431 StdErr = 0.029 nFit = 6 nDrop = 0 Predicted Z = 2.881 StdErr = 0.013 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.019, -0.025 um; position 0.116, 0.456 Drift reached 7.2 A/sec after 5.2 sec, going on Running script # 14 -10.4 3.7 unbinned pixels; 2.4 3.0 nm along two shift axes; -1.0 -3.8 nm on specimen axes Rate = 19.29993 A/sec -7.6 2.6 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.7 -2.7 nm on specimen axes Rate = 14.02633 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_023_12.0_Oct22_15.16.08.tif Record image shifted -0.001, -0.008 um Tracking reference shifted by -21.6,3.7 pixels, -0.001,-0.008 um Saved Z = 23 at 15.35 min Tilt = 12.00 Mean = 139 Exp = 1.515 X/Y/Z = 0.120 0.474 2.881 Errors in X/Y Prediction 0.023 0.043 um, 1.7 3.3 % Predicted X = 0.122 StdErr = 0.025 nFit = 3 nDrop = 0 Predicted Y = 0.464 StdErr = 0.052 nFit = 5 nDrop = 0 Predicted Z = 2.784 StdErr = 0.015 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, 0.066 um; position 0.107, 0.399 Drift reached 4.8 A/sec after 5.2 sec, going on Measured defocus = -3.53 microns changed by 0.03 to target drift = 0.15 nm/sec Tracking image shifted -0.002, 0.006 um; position 0.111, 0.391 Running script # 14 -0.4 2.2 unbinned pixels; 0.8 0.1 nm along two shift axes; -0.8 -0.2 nm on specimen axes Rate = 3.90224 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_024_14.0_Oct22_15.16.51.tif Record image shifted 0.001, 0.009 um Tracking reference shifted by 26.5,-4.9 pixels, 0.001,0.009 um Saved Z = 24 at 16.06 min Tilt = 14.00 Mean = 140 Exp = 1.526 X/Y/Z = 0.111 0.382 2.810 Errors in X/Y Prediction -0.010 -0.083 um, 0.8 6.3 % Error in Z Prediction 0.026 Predicted X = 0.127 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = -0.661 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 4.557 StdErr = 0.007 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, 0.071 um; position 0.130, -0.732 Drift reached 2.0 A/sec after 5.2 sec, going on Running script # 14 0.2 1.4 unbinned pixels; 0.4 0.2 nm along two shift axes; -0.5 0.0 nm on specimen axes Rate = 2.47904 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_025_-36.0_Oct22_15.17.24.tif Record image shifted 0.001, -0.002 um Tracking reference shifted by -6.1,-2.6 pixels, 0.001,-0.002 um Saved Z = 25 at 16.61 min Tilt = -36.00 Mean = 138 Exp = 1.595 X/Y/Z = 0.130 -0.730 4.557 Errors in X/Y Prediction 0.003 -0.070 um, 0.2 5.3 % Predicted X = 0.135 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = -0.890 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 4.583 StdErr = 0.008 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, -0.101 um; position 0.126, -0.789 Drift reached 1.5 A/sec after 5.2 sec, going on Measured defocus = -3.53 microns changed by 0.03 to target drift = 0.08 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.128, -0.793 Running script # 14 1.1 0.5 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.03935 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_026_-38.0_Oct22_15.18.06.tif Record image shifted -0.001, 0.003 um Tracking reference shifted by 8.4,1.7 pixels, -0.001,0.003 um Saved Z = 26 at 17.31 min Tilt = -38.00 Mean = 139 Exp = 1.615 X/Y/Z = 0.129 -0.796 4.609 Errors in X/Y Prediction -0.006 0.094 um, 0.4 7.2 % Error in Z Prediction 0.026 Predicted X = 0.125 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Y = 0.329 StdErr = 0.055 nFit = 6 nDrop = 0 Predicted Z = 2.715 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.008, -0.148 um; position 0.133, 0.477 Drift reached 7.4 A/sec after 5.1 sec, going on Running script # 14 -7.9 3.6 unbinned pixels; 2.1 2.2 nm along two shift axes; -1.0 -2.9 nm on specimen axes Rate = 15.30391 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_027_16.0_Oct22_15.18.42.tif Record image shifted -0.003, -0.014 um Tracking reference shifted by -38.8,12.6 pixels, -0.003,-0.014 um Saved Z = 27 at 17.91 min Tilt = 16.00 Mean = 138 Exp = 1.524 X/Y/Z = 0.139 0.498 2.715 Errors in X/Y Prediction 0.014 0.169 um, 1.1 12.9 % Predicted X = 0.142 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Y = 0.526 StdErr = 0.134 nFit = 5 nDrop = 0 Predicted Z = 2.625 StdErr = 0.015 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.027, 0.156 um; position 0.115, 0.370 Drift reached 3.9 A/sec after 5.2 sec, going on Measured defocus = -3.55 microns changed by 0.05 to target drift = 0.21 nm/sec Tracking image shifted -0.001, -0.003 um; position 0.118, 0.374 Running script # 14 -1.6 2.0 unbinned pixels; 0.8 0.3 nm along two shift axes; -0.7 -0.6 nm on specimen axes Rate = 4.44947 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_028_18.0_Oct22_15.19.25.tif Record image shifted 0.003, 0.012 um Tracking reference shifted by 33.1,-12.9 pixels, 0.003,0.012 um Saved Z = 28 at 18.63 min Tilt = 18.00 Mean = 141 Exp = 1.545 X/Y/Z = 0.115 0.363 2.679 Errors in X/Y Prediction -0.027 -0.163 um, 2.1 12.5 % Error in Z Prediction 0.054 Predicted X = 0.140 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.938 StdErr = 0.050 nFit = 6 nDrop = 0 Predicted Z = 4.641 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.009, -0.001 um; position 0.131, -0.936 Drift reached 0.5 A/sec after 5.2 sec, going on Running script # 14 0.0 1.1 unbinned pixels; 0.4 0.1 nm along two shift axes; -0.4 -0.0 nm on specimen axes Rate = 1.98173 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_029_-40.0_Oct22_15.20.00.tif Record image shifted -0.001, -0.001 um Tracking reference shifted by -3.0,2.4 pixels, -0.001,-0.001 um Saved Z = 29 at 19.21 min Tilt = -40.00 Mean = 138 Exp = 1.631 X/Y/Z = 0.133 -0.935 4.641 Errors in X/Y Prediction -0.007 0.002 um, 0.6 0.2 % Predicted X = 0.124 StdErr = 0.015 nFit = 4 nDrop = 0 Predicted Y = -1.053 StdErr = 0.047 nFit = 6 nDrop = 0 Predicted Z = 4.675 StdErr = 0.012 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.006, -0.046 um; position 0.130, -1.007 Drift reached 1.3 A/sec after 5.4 sec, going on Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.004 um; position 0.131, -1.012 Running script # 14 0.5 0.4 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 1.09542 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_030_-42.0_Oct22_15.20.47.tif Record image shifted 0.001, 0.002 um Tracking reference shifted by 5.0,-4.3 pixels, 0.001,0.002 um Saved Z = 30 at 19.99 min Tilt = -42.00 Mean = 138 Exp = 1.660 X/Y/Z = 0.130 -1.014 4.691 Errors in X/Y Prediction 0.006 0.039 um, 0.5 3.0 % Error in Z Prediction 0.016 Predicted X = 0.131 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -1.093 StdErr = 0.062 nFit = 5 nDrop = 0 Predicted Z = 4.723 StdErr = 0.011 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, 0.046 um; position 0.124, -1.139 Drift reached 1.4 A/sec after 5.4 sec, going on Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.03 nm/sec Tracking image shifted 0.001, -0.000 um; position 0.124, -1.138 Running script # 14 0.0 0.5 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 -0.0 nm on specimen axes Rate = 0.83506 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_031_-44.0_Oct22_15.21.28.tif Record image shifted -0.000, -0.001 um Tracking reference shifted by -2.7,1.2 pixels, -0.000,-0.001 um Saved Z = 31 at 20.69 min Tilt = -44.00 Mean = 138 Exp = 1.680 X/Y/Z = 0.124 -1.137 4.685 Errors in X/Y Prediction -0.007 -0.044 um, 0.5 3.4 % Error in Z Prediction -0.038 Predicted X = 0.126 StdErr = 0.038 nFit = 3 nDrop = 0 Predicted Y = 0.320 StdErr = 0.172 nFit = 5 nDrop = 0 Predicted Z = 2.591 StdErr = 0.020 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.012, -0.122 um; position 0.138, 0.442 Drift reached 6.5 A/sec after 5.2 sec, going on Running script # 14 -8.5 4.3 unbinned pixels; 2.4 2.3 nm along two shift axes; -1.3 -3.1 nm on specimen axes Rate = 16.74216 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_032_20.0_Oct22_15.22.05.tif Record image shifted -0.003, -0.011 um Tracking reference shifted by -30.1,11.4 pixels, -0.003,-0.011 um Saved Z = 32 at 21.29 min Tilt = 20.00 Mean = 138 Exp = 1.539 X/Y/Z = 0.144 0.459 2.591 Errors in X/Y Prediction 0.018 0.139 um, 1.4 10.6 % Predicted X = 0.137 StdErr = 0.039 nFit = 3 nDrop = 0 Predicted Y = 0.441 StdErr = 0.140 nFit = 6 nDrop = 0 Predicted Z = 2.512 StdErr = 0.023 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, 0.098 um; position 0.145, 0.343 Drift reached 4.2 A/sec after 5.2 sec, going on Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.19 nm/sec Tracking image shifted -0.004, -0.004 um; position 0.151, 0.349 Running script # 14 -1.0 2.1 unbinned pixels; 0.8 0.1 nm along two shift axes; -0.7 -0.4 nm on specimen axes Rate = 4.11301 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_033_22.0_Oct22_15.22.48.tif Record image shifted 0.004, 0.010 um Tracking reference shifted by 27.5,-12.4 pixels, 0.004,0.010 um Saved Z = 33 at 22.01 min Tilt = 22.00 Mean = 140 Exp = 1.564 X/Y/Z = 0.148 0.339 2.515 Errors in X/Y Prediction 0.011 -0.102 um, 0.8 7.8 % Error in Z Prediction 0.003 Predicted X = 0.168 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.250 StdErr = 0.187 nFit = 5 nDrop = 0 Predicted Z = 2.438 StdErr = 0.018 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.027, -0.123 um; position 0.142, 0.373 Drift reached 5.8 A/sec after 5.1 sec, going on Running script # 14 -7.3 3.3 unbinned pixels; 2.0 2.0 nm along two shift axes; -0.9 -2.7 nm on specimen axes Rate = 14.0677 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_034_24.0_Oct22_15.23.19.tif Record image shifted -0.004, -0.011 um Tracking reference shifted by -30.0,13.1 pixels, -0.004,-0.011 um Saved Z = 34 at 22.53 min Tilt = 24.00 Mean = 138 Exp = 1.567 X/Y/Z = 0.148 0.390 2.438 Errors in X/Y Prediction -0.020 0.140 um, 1.6 10.7 % Predicted X = 0.113 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = -1.226 StdErr = 0.049 nFit = 6 nDrop = 0 Predicted Z = 4.736 StdErr = 0.028 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.034, 0.014 um; position 0.147, -1.240 Drift reached 2.7 A/sec after 5.4 sec, going on Running script # 14 2.9 1.0 unbinned pixels; 0.0 1.1 nm along two shift axes; -0.5 1.0 nm on specimen axes Rate = 5.45547 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_035_-46.0_Oct22_15.23.55.tif Record image shifted -0.000, 0.004 um Tracking reference shifted by 10.5,-0.4 pixels, -0.000,0.004 um Saved Z = 35 at 23.12 min Tilt = -46.00 Mean = 138 Exp = 1.708 X/Y/Z = 0.149 -1.246 4.736 Errors in X/Y Prediction 0.035 -0.020 um, 2.7 1.5 % Predicted X = 0.153 StdErr = 0.022 nFit = 3 nDrop = 0 Predicted Y = -1.348 StdErr = 0.041 nFit = 5 nDrop = 0 Predicted Z = 4.769 StdErr = 0.032 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.012, 0.042 um; position 0.141, -1.390 Drift reached 1.3 A/sec after 5.4 sec, going on Measured defocus = -3.43 microns changed by -0.07 to target drift = 0.06 nm/sec Tracking image shifted 0.000, 0.000 um; position 0.142, -1.390 Running script # 14 0.3 1.1 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.4 0.1 nm on specimen axes Rate = 2.00308 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_036_-48.0_Oct22_15.24.36.tif Record image shifted 0.001, -0.002 um Tracking reference shifted by -4.5,-1.1 pixels, 0.001,-0.002 um Saved Z = 36 at 23.81 min Tilt = -48.00 Mean = 137 Exp = 1.735 X/Y/Z = 0.142 -1.389 4.695 Errors in X/Y Prediction -0.011 -0.041 um, 0.9 3.2 % Error in Z Prediction -0.074 Predicted X = 0.156 StdErr = 0.023 nFit = 3 nDrop = 0 Predicted Y = -1.543 StdErr = 0.023 nFit = 5 nDrop = 0 Predicted Z = 4.727 StdErr = 0.041 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.017, -0.049 um; position 0.138, -1.494 Drift reached 1.8 A/sec after 5.4 sec, going on Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.002 um; position 0.139, -1.497 Running script # 14 0.8 0.4 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.61601 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_037_-50.0_Oct22_15.25.18.tif Record image shifted 0.000, 0.001 um Tracking reference shifted by 1.8,-0.7 pixels, 0.000,0.001 um Saved Z = 37 at 24.51 min Tilt = -50.00 Mean = 137 Exp = 1.773 X/Y/Z = 0.139 -1.498 4.695 Errors in X/Y Prediction -0.017 0.045 um, 1.3 3.5 % Error in Z Prediction -0.032 Predicted X = 0.114 StdErr = 0.023 nFit = 4 nDrop = 0 Predicted Y = -1.625 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 4.697 StdErr = 0.006 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.020, 0.004 um; position 0.134, -1.629 Drift reached 0.4 A/sec after 5.6 sec, going on Running script # 14 0.3 0.8 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.57574 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_038_-52.0_Oct22_15.25.48.tif Record image shifted -0.001, 0.000 um Tracking reference shifted by 0.6,3.6 pixels, -0.001,0.000 um Saved Z = 38 at 25.02 min Tilt = -52.00 Mean = 136 Exp = 1.811 X/Y/Z = 0.136 -1.629 4.697 Errors in X/Y Prediction 0.022 -0.004 um, 1.7 0.3 % Predicted X = 0.129 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Y = 0.415 StdErr = 0.170 nFit = 5 nDrop = 0 Predicted Z = 2.361 StdErr = 0.020 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.036, 0.016 um; position 0.165, 0.399 Drift reached 4.1 A/sec after 5.2 sec, going on Measured defocus = -3.40 microns changed by -0.10 to target drift = 0.28 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.19 nm/sec Tracking image shifted -0.003, -0.013 um; position 0.169, 0.414 Running script # 14 -3.1 1.1 unbinned pixels; 0.7 0.9 nm along two shift axes; -0.3 -1.1 nm on specimen axes Rate = 5.76414 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_039_26.0_Oct22_15.26.46.tif Record image shifted 0.005, 0.009 um Tracking reference shifted by 23.4,-16.7 pixels, 0.005,0.009 um Saved Z = 39 at 25.97 min Tilt = 26.00 Mean = 140 Exp = 1.591 X/Y/Z = 0.164 0.407 2.255 Errors in X/Y Prediction 0.035 -0.008 um, 2.7 0.6 % Error in Z Prediction -0.106 Predicted X = 0.181 StdErr = 0.014 nFit = 4 nDrop = 0 Predicted Y = 0.403 StdErr = 0.153 nFit = 5 nDrop = 0 Predicted Z = 2.165 StdErr = 0.061 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.034, 0.067 um; position 0.147, 0.336 Drift reached 3.6 A/sec after 5.2 sec, going on Measured defocus = -3.57 microns changed by 0.07 to target drift = 0.20 nm/sec Tracking image shifted -0.003, -0.004 um; position 0.152, 0.341 Running script # 14 -1.5 2.4 unbinned pixels; 1.0 0.2 nm along two shift axes; -0.8 -0.6 nm on specimen axes Rate = 5.05946 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_040_28.0_Oct22_15.27.29.tif Record image shifted -0.000, 0.001 um Tracking reference shifted by 4.0,1.1 pixels, -0.000,0.001 um Saved Z = 40 at 26.70 min Tilt = 28.00 Mean = 138 Exp = 1.596 X/Y/Z = 0.154 0.340 2.234 Errors in X/Y Prediction -0.027 -0.063 um, 2.1 4.8 % Error in Z Prediction 0.068 Predicted X = 0.161 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.317 StdErr = 0.112 nFit = 6 nDrop = 0 Predicted Z = 2.111 StdErr = 0.058 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.011, -0.032 um; position 0.150, 0.349 Drift reached 5.6 A/sec after 5.2 sec, going on Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.28 nm/sec Tracking image shifted -0.006, -0.014 um; position 0.157, 0.365 Running script # 14 -4.8 1.7 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.5 -1.7 nm on specimen axes Rate = 8.86857 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_041_30.0_Oct22_15.28.12.tif Record image shifted 0.000, -0.001 um Tracking reference shifted by -4.2,-0.8 pixels, 0.000,-0.001 um Saved Z = 41 at 27.41 min Tilt = 30.00 Mean = 138 Exp = 1.617 X/Y/Z = 0.157 0.369 2.103 Errors in X/Y Prediction -0.004 0.051 um, 0.3 3.9 % Error in Z Prediction -0.007 Predicted X = 0.152 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.386 StdErr = 0.098 nFit = 6 nDrop = 0 Predicted Z = 2.011 StdErr = 0.045 nFit = 5 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, 0.042 um; position 0.151, 0.344 Drift reached 2.9 A/sec after 5.4 sec, going on Running script # 14 -2.0 4.1 unbinned pixels; 1.6 0.2 nm along two shift axes; -1.4 -0.8 nm on specimen axes Rate = 8.0082 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_042_32.0_Oct22_15.28.43.tif Record image shifted -0.003, -0.002 um Tracking reference shifted by -5.0,7.9 pixels, -0.003,-0.002 um Saved Z = 42 at 27.94 min Tilt = 32.00 Mean = 138 Exp = 1.639 X/Y/Z = 0.156 0.348 2.011 Errors in X/Y Prediction 0.005 -0.039 um, 0.4 3.0 % Predicted X = 0.133 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -1.757 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 4.699 StdErr = 0.007 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.031, 0.001 um; position 0.165, -1.758 Drift reached 1.5 A/sec after 5.7 sec, going on Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.09 nm/sec Tracking image shifted -0.002, 0.004 um; position 0.167, -1.763 Running script # 14 1.1 0.8 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.3 0.4 nm on specimen axes Rate = 2.37474 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_043_-54.0_Oct22_15.29.33.tif Record image shifted 0.001, 0.003 um Tracking reference shifted by 7.7,-4.0 pixels, 0.001,0.003 um Saved Z = 43 at 28.77 min Tilt = -54.00 Mean = 137 Exp = 1.858 X/Y/Z = 0.166 -1.766 4.706 Errors in X/Y Prediction 0.033 -0.009 um, 2.5 0.7 % Error in Z Prediction 0.007 Predicted X = 0.174 StdErr = 0.025 nFit = 3 nDrop = 0 Predicted Y = -1.894 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 4.709 StdErr = 0.003 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.018, -0.016 um; position 0.156, -1.878 Drift reached 1.4 A/sec after 5.7 sec, going on Running script # 14 1.8 2.6 unbinned pixels; 0.7 0.9 nm along two shift axes; -1.0 0.5 nm on specimen axes Rate = 5.55279 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_044_-56.0_Oct22_15.30.09.tif Record image shifted -0.001, -0.000 um Tracking reference shifted by -0.6,2.5 pixels, -0.001,-0.000 um Saved Z = 44 at 29.38 min Tilt = -56.00 Mean = 136 Exp = 1.902 X/Y/Z = 0.158 -1.879 4.709 Errors in X/Y Prediction -0.016 0.015 um, 1.2 1.2 % Predicted X = 0.175 StdErr = 0.028 nFit = 3 nDrop = 0 Predicted Y = -2.013 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 4.713 StdErr = 0.003 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.018, -0.071 um; position 0.157, -1.942 Drift reached 1.8 A/sec after 5.9 sec, going on Measured defocus = -3.41 microns changed by -0.09 to target drift = 0.06 nm/sec Tracking image shifted -0.001, 0.001 um; position 0.158, -1.944 Running script # 14 0.9 0.7 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 1.97789 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_045_-58.0_Oct22_15.30.54.tif Trimming A by 35 40, reference by 20 20 Record image shifted 0.001, 0.002 um Tracking reference shifted by 4.1,-2.9 pixels, 0.001,0.002 um Saved Z = 45 at 30.13 min Tilt = -58.00 Mean = 136 Exp = 1.965 X/Y/Z = 0.158 -1.946 4.625 Errors in X/Y Prediction -0.017 0.067 um, 1.3 5.2 % Error in Z Prediction -0.088 Predicted X = 0.152 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -2.012 StdErr = 0.027 nFit = 5 nDrop = 0 Predicted Z = 4.633 StdErr = 0.045 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, 0.011 um; position 0.154, -2.022 Drift reached 0.7 A/sec after 5.9 sec, going on Running script # 14 0.6 1.4 unbinned pixels; 0.4 0.4 nm along two shift axes; -0.5 0.2 nm on specimen axes Rate = 2.68089 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_046_-60.0_Oct22_15.31.26.tif Trimming A by 55 55, reference by 50 50 Record image shifted -0.001, 0.000 um Tracking reference shifted by 1.4,3.3 pixels, -0.001,0.000 um Saved Z = 46 at 30.66 min Tilt = -60.00 Mean = 135 Exp = 2.027 X/Y/Z = 0.157 -2.023 4.633 Errors in X/Y Prediction 0.004 -0.011 um, 0.3 0.9 % Predicted X = 0.156 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -2.056 StdErr = 0.023 nFit = 5 nDrop = 0 Predicted Z = 4.620 StdErr = 0.051 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.042 um; position 0.156, -2.098 Drift reached 1.0 A/sec after 6.1 sec, going on Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.05 nm/sec Tracking image shifted -0.000, 0.001 um; position 0.156, -2.099 Running script # 14 0.4 0.7 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.38138 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_047_-62.0_Oct22_15.32.12.tif Trimming A by 30 20, reference by 60 65 Record image shifted 0.001, 0.003 um Tracking reference shifted by 7.8,-4.7 pixels, 0.001,0.003 um Saved Z = 47 at 31.43 min Tilt = -62.00 Mean = 134 Exp = 2.108 X/Y/Z = 0.155 -2.102 4.538 Errors in X/Y Prediction -0.001 -0.046 um, 0.1 3.5 % Error in Z Prediction -0.083 Predicted X = 0.158 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.343 StdErr = 0.067 nFit = 5 nDrop = 0 Predicted Z = 1.914 StdErr = 0.049 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.033, -0.049 um; position 0.191, 0.392 Drift reached 3.3 A/sec after 5.5 sec, going on Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.30 nm/sec Tracking image shifted -0.003, -0.008 um; position 0.195, 0.401 Running script # 14 -5.5 2.2 unbinned pixels; 1.4 1.6 nm along two shift axes; -0.6 -2.0 nm on specimen axes Rate = 10.42424 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_048_34.0_Oct22_15.33.03.tif Record image shifted 0.003, 0.001 um Tracking reference shifted by 1.5,-9.3 pixels, 0.003,0.001 um Saved Z = 48 at 32.27 min Tilt = 34.00 Mean = 139 Exp = 1.660 X/Y/Z = 0.192 0.403 1.875 Errors in X/Y Prediction 0.034 0.059 um, 2.6 4.5 % Error in Z Prediction -0.040 Predicted X = 0.203 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Y = 0.470 StdErr = 0.055 nFit = 5 nDrop = 0 Predicted Z = 1.791 StdErr = 0.066 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.054, 0.125 um; position 0.149, 0.345 Drift reached 4.1 A/sec after 5.4 sec, going on Measured defocus = -3.53 microns changed by 0.03 to target drift = 0.18 nm/sec Tracking image shifted -0.001, -0.005 um; position 0.152, 0.352 Running script # 14 -1.2 2.1 unbinned pixels; 0.8 0.1 nm along two shift axes; -0.7 -0.5 nm on specimen axes Rate = 4.17267 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_049_36.0_Oct22_15.33.48.tif Record image shifted -0.000, 0.002 um Tracking reference shifted by 6.0,-0.1 pixels, -0.000,0.002 um Saved Z = 49 at 33.01 min Tilt = 36.00 Mean = 138 Exp = 1.677 X/Y/Z = 0.153 0.350 1.820 Errors in X/Y Prediction -0.051 -0.120 um, 3.9 9.2 % Error in Z Prediction 0.029 Predicted X = 0.163 StdErr = 0.056 nFit = 3 nDrop = 0 Predicted Y = 0.335 StdErr = 0.069 nFit = 5 nDrop = 0 Predicted Z = 1.712 StdErr = 0.050 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.002 um; position 0.159, 0.338 Drift reached 2.1 A/sec after 5.4 sec, going on Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.15 nm/sec Tracking image shifted -0.004, -0.004 um; position 0.164, 0.343 Running script # 14 -1.6 1.8 unbinned pixels; 0.8 0.3 nm along two shift axes; -0.6 -0.6 nm on specimen axes Rate = 4.24642 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_050_38.0_Oct22_15.34.32.tif Record image shifted 0.000, -0.001 um Tracking reference shifted by -2.1,-0.3 pixels, 0.000,-0.001 um Saved Z = 50 at 33.75 min Tilt = 38.00 Mean = 138 Exp = 1.705 X/Y/Z = 0.164 0.344 1.686 Errors in X/Y Prediction 0.001 0.009 um, 0.1 0.7 % Error in Z Prediction -0.026 Predicted X = 0.142 StdErr = 0.038 nFit = 3 nDrop = 0 Predicted Y = 0.310 StdErr = 0.069 nFit = 5 nDrop = 0 Predicted Z = 1.582 StdErr = 0.032 nFit = 5 nDrop = 0 Need regular tracking - Tracking image shifted -0.022, 0.032 um; position 0.164, 0.278 Drift reached 2.2 A/sec after 5.4 sec, going on Running script # 14 -1.9 3.4 unbinned pixels; 1.4 0.2 nm along two shift axes; -1.1 -0.8 nm on specimen axes Rate = 6.87685 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_051_40.0_Oct22_15.35.04.tif Trimming A by 20 25, reference by 20 20 Record image shifted -0.002, -0.003 um Tracking reference shifted by -9.3,7.6 pixels, -0.002,-0.003 um Saved Z = 51 at 34.29 min Tilt = 40.00 Mean = 137 Exp = 1.735 X/Y/Z = 0.169 0.283 1.582 Errors in X/Y Prediction 0.027 -0.027 um, 2.0 2.1 % Predicted X = 0.178 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = 0.196 StdErr = 0.058 nFit = 5 nDrop = 0 Predicted Z = 1.476 StdErr = 0.036 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.081 um; position 0.173, 0.277 Drift reached 4.5 A/sec after 5.4 sec, going on Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.29 nm/sec Tracking image shifted -0.003, -0.012 um; position 0.177, 0.292 Running script # 14 -4.9 1.4 unbinned pixels; 1.1 1.5 nm along two shift axes; -0.4 -1.8 nm on specimen axes Rate = 9.02506 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_052_42.0_Oct22_15.35.49.tif Trimming A by 25 30, reference by 20 25 Record image shifted 0.003, 0.000 um Tracking reference shifted by 0.5,-7.8 pixels, 0.003,0.000 um Saved Z = 52 at 35.03 min Tilt = 42.00 Mean = 137 Exp = 1.774 X/Y/Z = 0.175 0.293 1.447 Errors in X/Y Prediction -0.003 0.097 um, 0.2 7.4 % Error in Z Prediction -0.029 Predicted X = 0.152 StdErr = 0.000 nFit = 4 nDrop = 0 Predicted Y = -2.159 StdErr = 0.028 nFit = 5 nDrop = 0 Predicted Z = 4.497 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.019, 0.032 um; position 0.171, -2.191 Drift reached 2.4 A/sec after 6.0 sec, going on Running script # 14 2.9 1.0 unbinned pixels; 0.0 1.1 nm along two shift axes; -0.4 1.0 nm on specimen axes Rate = 5.33799 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_053_-64.0_Oct22_15.36.27.tif Trimming A by 85 85, reference by 75 80 Record image shifted -0.001, 0.003 um Tracking reference shifted by 9.6,0.6 pixels, -0.001,0.003 um Saved Z = 53 at 35.68 min Tilt = -64.00 Mean = 132 Exp = 2.195 X/Y/Z = 0.173 -2.196 4.497 Errors in X/Y Prediction 0.020 -0.038 um, 1.6 2.9 % Predicted X = 0.195 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Y = -2.295 StdErr = 0.005 nFit = 5 nDrop = 1 Predicted Z = 4.455 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.028, -0.011 um; position 0.167, -2.284 Drift reached 1.5 A/sec after 6.2 sec, going on Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.05 nm/sec Tracking image shifted -0.001, -0.000 um; position 0.169, -2.284 Running script # 14 0.1 0.9 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.0 nm on specimen axes Rate = 1.67557 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_054_-66.0_Oct22_15.37.13.tif Trimming A by 95 100, reference by 90 95 Record image shifted 0.001, 0.001 um Tracking reference shifted by 3.0,-2.5 pixels, 0.001,0.001 um Saved Z = 54 at 36.45 min Tilt = -66.00 Mean = 132 Exp = 2.319 X/Y/Z = 0.168 -2.285 4.456 Errors in X/Y Prediction -0.027 0.010 um, 2.1 0.7 % Error in Z Prediction 0.001 Predicted X = 0.179 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = -2.386 StdErr = 0.008 nFit = 5 nDrop = 0 Predicted Z = 4.414 StdErr = 0.002 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, -0.037 um; position 0.169, -2.349 Drift reached 0.9 A/sec after 6.4 sec, going on Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.04 nm/sec Tracking image shifted -0.001, 0.001 um; position 0.171, -2.350 Running script # 14 Trimming A by 21 20 20 25, reference by 21 20 20 25 Trimming A by 21 20 20 25, reference by 21 20 20 25 0.4 0.3 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.1 0.1 nm on specimen axes Rate = 0.94563 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_055_-68.0_Oct22_15.38.00.tif Trimming A by 23 150, reference by 22 145 Record image shifted 0.000, -0.003 um Tracking reference shifted by -7.6,-0.2 pixels, 0.000,-0.003 um Saved Z = 55 at 37.23 min Tilt = -68.00 Mean = 130 Exp = 2.457 X/Y/Z = 0.171 -2.347 4.456 Errors in X/Y Prediction -0.008 0.038 um, 0.6 2.9 % Error in Z Prediction 0.042 Predicted X = 0.180 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.284 StdErr = 0.047 nFit = 5 nDrop = 0 Predicted Z = 1.347 StdErr = 0.046 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.021, 0.065 um; position 0.201, 0.219 Drift reached 4.2 A/sec after 5.6 sec, going on Running script # 14 -8.2 2.8 unbinned pixels; 1.9 2.4 nm along two shift axes; -0.7 -3.0 nm on specimen axes Rate = 15.23575 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_056_44.0_Oct22_15.38.40.tif Trimming A by 20 35, reference by 20 35 Record image shifted 0.001, -0.008 um Tracking reference shifted by -22.3,-1.1 pixels, 0.001,-0.008 um Saved Z = 56 at 37.88 min Tilt = 44.00 Mean = 136 Exp = 1.807 X/Y/Z = 0.201 0.232 1.347 Errors in X/Y Prediction 0.021 -0.052 um, 1.6 4.0 % Predicted X = 0.214 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = 0.194 StdErr = 0.055 nFit = 5 nDrop = 0 Predicted Z = 1.237 StdErr = 0.054 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.041, 0.025 um; position 0.173, 0.169 Drift reached 3.3 A/sec after 5.6 sec, going on Measured defocus = -3.53 microns changed by 0.03 to target drift = 0.12 nm/sec Tracking image shifted -0.001, -0.006 um; position 0.175, 0.177 Running script # 14 -1.6 1.4 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.5 -0.6 nm on specimen axes Rate = 3.69302 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_057_46.0_Oct22_15.39.25.tif Trimming A by 20 30, reference by 25 35 Record image shifted -0.001, 0.009 um Tracking reference shifted by 24.9,0.1 pixels, -0.001,0.009 um Saved Z = 57 at 38.62 min Tilt = 46.00 Mean = 137 Exp = 1.857 X/Y/Z = 0.176 0.168 1.260 Errors in X/Y Prediction -0.038 -0.026 um, 2.9 2.0 % Error in Z Prediction 0.024 Predicted X = 0.185 StdErr = 0.039 nFit = 3 nDrop = 0 Predicted Y = 0.105 StdErr = 0.057 nFit = 5 nDrop = 0 Predicted Z = 1.134 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.013 um; position 0.184, 0.119 Drift reached 4.0 A/sec after 5.6 sec, going on Running script # 14 -7.3 1.6 unbinned pixels; 1.4 2.2 nm along two shift axes; -0.3 -2.6 nm on specimen axes Rate = 13.05155 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_058_48.0_Oct22_15.39.57.tif Trimming A by 20 30, reference by 20 20 Record image shifted -0.002, -0.007 um Tracking reference shifted by -19.9,8.6 pixels, -0.002,-0.007 um Saved Z = 58 at 39.17 min Tilt = 48.00 Mean = 135 Exp = 1.899 X/Y/Z = 0.188 0.131 1.134 Errors in X/Y Prediction 0.002 0.025 um, 0.2 1.9 % Predicted X = 0.175 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Y = 0.044 StdErr = 0.052 nFit = 5 nDrop = 0 Predicted Z = 1.022 StdErr = 0.037 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.008, -0.058 um; position 0.183, 0.102 Drift reached 2.6 A/sec after 5.8 sec, going on Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.11 nm/sec Tracking image shifted -0.004, -0.006 um; position 0.188, 0.109 Running script # 14 -1.3 1.2 unbinned pixels; 0.5 0.3 nm along two shift axes; -0.4 -0.5 nm on specimen axes Rate = 3.03948 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo5_L1G1_059_50.0_Oct22_15.40.43.tif Record image shifted 0.003, 0.007 um Tracking reference shifted by 19.8,-9.2 pixels, 0.003,0.007 um Saved Z = 59 at 39.94 min Tilt = 50.00 Mean = 137 Exp = 1.974 X/Y/Z = 0.185 0.103 1.034 2020/10/22 15:41 D:\Users\Ben\201022_Jans_cells\SEM\tomo5_L1G1.st 0 60 3838 3710 1708 -10.0 50.0 2.00 REG 39.9 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = -0.000, 0.000 Angle = 18.00, shift = -478.4, -52.0 pixels, 0.004, -1.099 um, IS = -0.004, 1.099 Angle = 11.00, shift = -542.3, -70.4 pixels, -0.021, -1.248 um, IS = 0.017, 2.347 Angle = 4.00, shift = -462.0, -62.2 pixels, -0.023, -1.064 um, IS = 0.040, 3.411 General solution delZ 7.66, delY 6.05, yZero 3.98 solution with assumed YZero 3.98: delZ 7.66, delY 6.05 Refining eucentricity: Fit to 4 positions gives tilt axis Z displacement of 7.66 microns and lateral displacement of -6.05 microns Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = -77.6, -0.9 pixels, 0.018, -0.176 um, IS = -0.018, 0.176 Angle = 11.00, shift = -96.6, -8.4 pixels, 0.006, -0.221 um, IS = -0.023, 0.398 Angle = 4.00, shift = -29.8, -1.4 pixels, 0.004, -0.068 um, IS = -0.028, 0.466 Angle = -4.00, shift = -37.4, -22.4 pixels, -0.041, -0.091 um, IS = 0.013, 0.556 Angle = -11.00, shift = -17.5, -15.9 pixels, -0.032, -0.044 um, IS = 0.045, 0.600 Angle = -18.00, shift = -60.8, -4.6 pixels, 0.005, -0.139 um, IS = 0.040, 0.739 Angle = -24.00, shift = -20.0, 1.8 pixels, 0.009, -0.045 um, IS = 0.031, 0.784 General solution delZ 0.89, delY 0.92, yZero 0.52 solution with assumed YZero 0.51: delZ 0.89, delY 0.77 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.89 microns and lateral displacement of -0.92 microns Measured defocus = -4.85 microns changed by 0.35 to target drift = 0.12 nm/sec Need regular tracking - Need autofocus Trimming A by 31 20 20 20, reference by 20 20 20 20 Tracking image shifted -0.000, 0.000 um; position 0.000, -0.000 Drift reached 0.4 A/sec after 5.2 sec, going on Measured defocus = 0.00 microns changed by -4.50 to target drift = 0.00 nm/sec Tracking image shifted -0.000, -0.000 um; position -0.000, 0.000 Running script # 14 Trimming A by 25 20 20 20, reference by 25 20 20 20 Trimming A by 20 20 20 20, reference by 25 20 20 20 -0.0 0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 -0.0 nm on specimen axes Rate = 0.04899 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo6_L5G1_000_-10.0_Oct22_15.57.30.tif Record image shifted -0.007, 0.012 um Tracking reference shifted by 35.5,15.8 pixels, -0.007,0.012 um Saved Z = 0 at -0.00 min Tilt = -10.00 Mean = 98 Exp = 1.650 X/Y/Z = 0.007 -0.012 -1.876 Need regular tracking - Need autofocus Trimming A by 45 45, reference by 20 20 Tracking image shifted -0.007, 0.015 um; position 0.013, -0.027 Drift reached 0.1 A/sec after 5.3 sec, going on Measured defocus = 0.01 microns changed by -4.51 to target drift = 0.00 nm/sec Trimming A by 75 75, reference by 20 20 Tracking image shifted 0.139, -0.359 um; position -0.126, 0.332 Running script # 14 0.0 0.1 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes Rate = 0.20081 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo6_L5G1_001_-12.0_Oct22_15.58.12.tif Record image shifted -0.138, 0.306 um Tracking reference shifted by 898.8,314.3 pixels, -0.138,0.306 um Getting a new tracking reference after Record to reduce reference conflicts This Record image has more than the specified fraction of data loss (lost fractions 0.097 in X, 0.209 in Y) Measured defocus = -15.36 microns changed by 10.86 to target drift = 0.20 nm/sec Measured defocus = -3.86 microns changed by -0.64 to target drift = 0.14 nm/sec 2020/10/22 16:01 D:\Users\Ben\201022_Jans_cells\SEM\tomo6_L5G1.st 0 1 3838 3710 28 -10.0 -10.0 2.00 REG 0.0 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Trimming A by 24 20 20 185, reference by 23 22 20 190 Need regular tracking - Need autofocus Tracking image shifted 0.004, 0.014 um; position 0.056, 0.280 Drift reached 1.3 A/sec after 5.2 sec, going on Measured defocus = -3.84 microns changed by -0.66 to target drift = 0.10 nm/sec Tracking image shifted -0.000, 0.005 um; position 0.056, 0.274 Running script # 14 0.8 1.3 unbinned pixels; 0.4 0.4 nm along two shift axes; -0.5 0.2 nm on specimen axes Rate = 2.75935 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_000_-10.0_Oct22_16.02.52.tif Record image shifted -0.014, -0.007 um Tracking reference shifted by -16.3,42.5 pixels, -0.014,-0.007 um Saved Z = 0 at 0.00 min Tilt = -10.00 Mean = 97 Exp = 1.650 X/Y/Z = 0.071 0.281 3.252 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.089 um; position 0.073, 0.369 Drift reached 1.2 A/sec after 5.2 sec, going on Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.06 nm/sec Tracking image shifted -0.002, -0.001 um; position 0.076, 0.371 Running script # 14 -0.1 1.0 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.76899 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_001_-12.0_Oct22_16.03.33.tif Record image shifted -0.000, -0.002 um Tracking reference shifted by -4.3,1.6 pixels, -0.000,-0.002 um Saved Z = 1 at 0.69 min Tilt = -12.00 Mean = 100 Exp = 1.698 X/Y/Z = 0.076 0.372 3.342 Predicted X = 0.081 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.464 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.433 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, -0.009 um; position 0.082, 0.473 Drift reached 0.4 A/sec after 5.3 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.04 nm/sec Tracking image shifted -0.002, -0.001 um; position 0.083, 0.473 Running script # 14 -0.2 1.0 unbinned pixels; 0.4 0.0 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.76756 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_002_-14.0_Oct22_16.04.15.tif Record image shifted -0.000, 0.000 um Tracking reference shifted by 1.1,0.3 pixels, -0.000,0.000 um Saved Z = 2 at 1.39 min Tilt = -14.00 Mean = 100 Exp = 1.706 X/Y/Z = 0.084 0.473 3.465 Errors in X/Y Prediction 0.003 0.009 um, 0.2 0.7 % Error in Z Prediction 0.033 Need regular tracking - Need autofocus Tracking image shifted -0.031, -0.052 um; position 0.102, 0.333 Drift reached 3.1 A/sec after 5.2 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.13 nm/sec Tracking image shifted -0.002, 0.006 um; position 0.104, 0.325 Running script # 14 1.4 0.8 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 2.84988 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_003_-8.0_Oct22_16.05.00.tif Record image shifted -0.000, -0.000 um Tracking reference shifted by -0.5,1.2 pixels, -0.000,-0.000 um Saved Z = 3 at 2.14 min Tilt = -8.00 Mean = 100 Exp = 1.698 X/Y/Z = 0.105 0.325 3.200 Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.036 um; position 0.105, 0.288 Drift reached 0.6 A/sec after 5.2 sec, going on Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.05 nm/sec Tracking image shifted -0.001, -0.003 um; position 0.105, 0.292 Running script # 14 -0.7 0.7 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.2 -0.3 nm on specimen axes Rate = 1.68382 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_004_-6.0_Oct22_16.05.43.tif Record image shifted -0.000, -0.002 um Tracking reference shifted by -5.8,1.1 pixels, -0.000,-0.002 um Saved Z = 4 at 2.86 min Tilt = -6.00 Mean = 100 Exp = 1.697 X/Y/Z = 0.106 0.294 3.100 Predicted X = 0.090 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.568 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Z = 3.567 StdErr = 0.024 nFit = 3 nDrop = 0 Drift reached 0.2 A/sec after 4.8 sec, going on Running script # 14 0.2 0.9 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.64846 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_005_-16.0_Oct22_16.06.14.tif Record image shifted -0.027, 0.041 um Saved Z = 5 at 3.38 min Tilt = -16.00 Mean = 99 Exp = 1.713 X/Y/Z = 0.117 0.527 3.567 Errors in X/Y Prediction 0.028 -0.041 um, 2.1 3.1 % Predicted X = 0.134 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = 0.612 StdErr = 0.035 nFit = 3 nDrop = 0 Predicted Z = 3.674 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.015, 0.057 um; position 0.119, 0.555 Drift reached 1.7 A/sec after 5.3 sec, going on Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.07 nm/sec Tracking image shifted 0.001, 0.003 um; position 0.119, 0.552 Running script # 14 0.8 0.7 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 1.88832 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_006_-18.0_Oct22_16.06.56.tif Record image shifted -0.000, 0.000 um Tracking reference shifted by 0.5,0.2 pixels, -0.000,0.000 um Saved Z = 6 at 4.08 min Tilt = -18.00 Mean = 99 Exp = 1.728 X/Y/Z = 0.119 0.551 3.641 Errors in X/Y Prediction -0.015 -0.061 um, 1.1 4.7 % Error in Z Prediction -0.032 Predicted X = 0.107 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.263 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.000 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.017, -0.100 um; position 0.124, 0.363 Drift reached 2.5 A/sec after 5.3 sec, going on Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.08 nm/sec Tracking image shifted -0.002, 0.002 um; position 0.127, 0.360 Running script # 14 0.4 1.0 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.1 nm on specimen axes Rate = 1.96076 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_007_-4.0_Oct22_16.07.42.tif Record image shifted -0.000, 0.001 um Tracking reference shifted by 2.4,1.0 pixels, -0.000,0.001 um Saved Z = 7 at 4.85 min Tilt = -4.00 Mean = 100 Exp = 1.695 X/Y/Z = 0.128 0.360 3.012 Errors in X/Y Prediction 0.021 0.096 um, 1.6 7.4 % Error in Z Prediction 0.012 Predicted X = 0.136 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.361 StdErr = 0.072 nFit = 3 nDrop = 0 Predicted Z = 2.917 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, 0.047 um; position 0.122, 0.314 Drift reached 1.2 A/sec after 5.3 sec, going on Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.06 nm/sec Tracking image shifted 0.000, -0.001 um; position 0.123, 0.315 Running script # 14 -0.0 0.9 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.0 nm on specimen axes Rate = 1.55137 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_008_-2.0_Oct22_16.08.27.tif Record image shifted -0.000, -0.000 um Tracking reference shifted by -1.2,0.4 pixels, -0.000,-0.000 um Saved Z = 8 at 5.59 min Tilt = -2.00 Mean = 100 Exp = 1.693 X/Y/Z = 0.123 0.315 2.892 Errors in X/Y Prediction -0.013 -0.046 um, 1.0 3.5 % Error in Z Prediction -0.025 Predicted X = 0.142 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Y = 0.595 StdErr = 0.022 nFit = 3 nDrop = 0 Predicted Z = 3.744 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, 0.049 um; position 0.148, 0.546 Drift reached 0.5 A/sec after 5.4 sec, going on Running script # 14 -0.5 0.8 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.2 nm on specimen axes Rate = 1.72286 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_009_-20.0_Oct22_16.09.00.tif Record image shifted 0.000, -0.003 um Tracking reference shifted by -7.9,-0.2 pixels, 0.000,-0.003 um Saved Z = 9 at 6.15 min Tilt = -20.00 Mean = 99 Exp = 1.738 X/Y/Z = 0.148 0.549 3.744 Errors in X/Y Prediction 0.006 -0.046 um, 0.5 3.6 % Predicted X = 0.159 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Y = 0.518 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Z = 3.842 StdErr = 0.039 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, -0.004 um; position 0.145, 0.522 Drift reached 1.4 A/sec after 5.4 sec, going on Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.09 nm/sec Tracking image shifted -0.000, 0.005 um; position 0.146, 0.517 Running script # 14 0.9 0.4 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.76582 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_010_-22.0_Oct22_16.09.43.tif Record image shifted -0.001, 0.001 um Tracking reference shifted by 3.9,1.4 pixels, -0.001,0.001 um Saved Z = 10 at 6.86 min Tilt = -22.00 Mean = 99 Exp = 1.752 X/Y/Z = 0.147 0.515 3.746 Errors in X/Y Prediction -0.012 -0.003 um, 0.9 0.2 % Error in Z Prediction -0.096 Predicted X = 0.136 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.345 StdErr = 0.082 nFit = 3 nDrop = 0 Predicted Z = 2.793 StdErr = 0.025 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, 0.002 um; position 0.142, 0.343 Drift reached 0.4 A/sec after 5.2 sec, going on Running script # 14 -0.1 1.1 unbinned pixels; 0.4 0.1 nm along two shift axes; -0.4 -0.1 nm on specimen axes Rate = 1.99164 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_011_-0.0_Oct22_16.10.18.tif Record image shifted -0.001, -0.000 um Tracking reference shifted by -1.0,1.7 pixels, -0.001,-0.000 um Saved Z = 11 at 7.43 min Tilt = -0.00 Mean = 100 Exp = 1.694 X/Y/Z = 0.143 0.343 2.793 Errors in X/Y Prediction 0.007 -0.001 um, 0.5 0.1 % Predicted X = 0.146 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = 0.323 StdErr = 0.054 nFit = 3 nDrop = 0 Predicted Z = 2.689 StdErr = 0.033 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.003 um; position 0.143, 0.326 Drift reached 3.2 A/sec after 5.2 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.09 nm/sec Tracking image shifted -0.001, 0.008 um; position 0.144, 0.317 Running script # 14 0.8 0.7 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.83082 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_012_2.0_Oct22_16.11.01.tif Record image shifted 0.001, 0.002 um Tracking reference shifted by 5.7,-3.4 pixels, 0.001,0.002 um Saved Z = 12 at 8.16 min Tilt = 2.00 Mean = 100 Exp = 1.699 X/Y/Z = 0.144 0.315 2.670 Errors in X/Y Prediction -0.002 -0.009 um, 0.2 0.7 % Error in Z Prediction -0.018 Predicted X = 0.158 StdErr = 0.039 nFit = 4 nDrop = 0 Predicted Y = 0.454 StdErr = 0.002 nFit = 5 nDrop = 0 Predicted Z = 3.828 StdErr = 0.046 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, -0.058 um; position 0.160, 0.512 Drift reached 0.9 A/sec after 5.4 sec, going on Running script # 14 -0.6 1.5 unbinned pixels; 0.6 0.0 nm along two shift axes; -0.5 -0.3 nm on specimen axes Rate = 2.91036 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_013_-24.0_Oct22_16.11.35.tif Record image shifted -0.001, -0.002 um Tracking reference shifted by -6.9,3.0 pixels, -0.001,-0.002 um Saved Z = 13 at 8.73 min Tilt = -24.00 Mean = 99 Exp = 1.772 X/Y/Z = 0.161 0.515 3.828 Errors in X/Y Prediction 0.003 0.061 um, 0.2 4.6 % Predicted X = 0.165 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = 0.472 StdErr = 0.035 nFit = 5 nDrop = 0 Predicted Z = 3.898 StdErr = 0.053 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.039 um; position 0.160, 0.512 Drift reached 1.8 A/sec after 5.4 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.161, 0.508 Running script # 14 0.8 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.90096 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_014_-26.0_Oct22_16.12.18.tif Record image shifted 0.001, 0.001 um Tracking reference shifted by 2.0,-2.6 pixels, 0.001,0.001 um Saved Z = 14 at 9.44 min Tilt = -26.00 Mean = 99 Exp = 1.789 X/Y/Z = 0.161 0.507 3.881 Errors in X/Y Prediction -0.005 0.034 um, 0.3 2.6 % Error in Z Prediction -0.017 Predicted X = 0.158 StdErr = 0.029 nFit = 4 nDrop = 0 Predicted Y = 0.325 StdErr = 0.085 nFit = 4 nDrop = 0 Predicted Z = 2.555 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, -0.035 um; position 0.162, 0.361 Drift reached 1.4 A/sec after 5.2 sec, going on Running script # 14 -0.8 1.2 unbinned pixels; 0.5 0.1 nm along two shift axes; -0.4 -0.3 nm on specimen axes Rate = 2.51518 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_015_4.0_Oct22_16.12.53.tif Record image shifted -0.001, -0.003 um Tracking reference shifted by -9.0,2.7 pixels, -0.001,-0.003 um Saved Z = 15 at 10.03 min Tilt = 4.00 Mean = 100 Exp = 1.702 X/Y/Z = 0.164 0.364 2.555 Errors in X/Y Prediction 0.006 0.039 um, 0.4 3.0 % Predicted X = 0.175 StdErr = 0.026 nFit = 4 nDrop = 0 Predicted Y = 0.407 StdErr = 0.053 nFit = 5 nDrop = 0 Predicted Z = 2.441 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, 0.076 um; position 0.162, 0.332 Drift reached 3.0 A/sec after 5.2 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.12 nm/sec Tracking image shifted 0.000, 0.003 um; position 0.163, 0.327 Running script # 14 1.0 1.0 unbinned pixels; 0.2 0.4 nm along two shift axes; -0.4 0.3 nm on specimen axes Rate = 2.45072 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_016_6.0_Oct22_16.13.38.tif Record image shifted 0.001, 0.004 um Tracking reference shifted by 12.1,-2.6 pixels, 0.001,0.004 um Saved Z = 16 at 10.77 min Tilt = 6.00 Mean = 99 Exp = 1.710 X/Y/Z = 0.162 0.322 2.460 Errors in X/Y Prediction -0.013 -0.085 um, 1.0 6.5 % Error in Z Prediction 0.018 Predicted X = 0.171 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = 0.477 StdErr = 0.030 nFit = 6 nDrop = 0 Predicted Z = 3.936 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.013, 0.005 um; position 0.184, 0.473 Drift reached 0.5 A/sec after 5.4 sec, going on Running script # 14 -0.6 0.7 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.2 -0.2 nm on specimen axes Rate = 1.58757 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_017_-28.0_Oct22_16.14.12.tif Record image shifted -0.001, -0.003 um Tracking reference shifted by -6.8,3.5 pixels, -0.001,-0.003 um Saved Z = 17 at 11.33 min Tilt = -28.00 Mean = 99 Exp = 1.810 X/Y/Z = 0.185 0.475 3.936 Errors in X/Y Prediction 0.014 -0.002 um, 1.1 0.1 % Predicted X = 0.193 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.464 StdErr = 0.030 nFit = 5 nDrop = 0 Predicted Z = 3.996 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.010, 0.047 um; position 0.183, 0.417 Drift reached 1.6 A/sec after 5.4 sec, going on Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.08 nm/sec Tracking image shifted 0.001, 0.004 um; position 0.183, 0.412 Running script # 14 1.2 1.6 unbinned pixels; 0.4 0.6 nm along two shift axes; -0.6 0.4 nm on specimen axes Rate = 3.47994 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_018_-30.0_Oct22_16.15.00.tif Record image shifted 0.001, 0.000 um Tracking reference shifted by 0.6,-1.6 pixels, 0.001,0.000 um Saved Z = 18 at 12.14 min Tilt = -30.00 Mean = 98 Exp = 1.836 X/Y/Z = 0.183 0.412 4.045 Errors in X/Y Prediction -0.010 -0.053 um, 0.7 4.0 % Error in Z Prediction 0.049 Predicted X = 0.170 StdErr = 0.028 nFit = 4 nDrop = 0 Predicted Y = 0.346 StdErr = 0.057 nFit = 6 nDrop = 0 Predicted Z = 2.350 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.015, 0.020 um; position 0.185, 0.326 Drift reached 5.4 A/sec after 5.3 sec, going on Running script # 14 -9.8 3.3 unbinned pixels; 2.2 2.8 nm along two shift axes; -0.9 -3.5 nm on specimen axes Rate = 18.08013 A/sec -9.1 3.6 unbinned pixels; 2.3 2.6 nm along two shift axes; -1.0 -3.3 nm on specimen axes Rate = 17.06592 A/sec -6.6 2.4 unbinned pixels; 1.6 1.9 nm along two shift axes; -0.6 -2.4 nm on specimen axes Rate = 12.23656 A/sec Drift is low enough after shot 3 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_019_8.0_Oct22_16.15.47.tif Record image shifted -0.001, -0.013 um Tracking reference shifted by -37.8,7.3 pixels, -0.001,-0.013 um Saved Z = 19 at 12.92 min Tilt = 8.00 Mean = 99 Exp = 1.721 X/Y/Z = 0.189 0.352 2.350 Errors in X/Y Prediction 0.019 0.006 um, 1.5 0.4 % Predicted X = 0.197 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Y = 0.345 StdErr = 0.049 nFit = 6 nDrop = 0 Predicted Z = 2.242 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, 0.010 um; position 0.183, 0.334 Drift reached 1.8 A/sec after 5.2 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.12 nm/sec Tracking image shifted -0.001, 0.004 um; position 0.184, 0.329 Running script # 14 2.0 1.4 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.5 0.7 nm on specimen axes Rate = 4.24764 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_020_10.0_Oct22_16.16.31.tif Record image shifted 0.001, 0.015 um Tracking reference shifted by 42.0,-7.5 pixels, 0.001,0.015 um Saved Z = 20 at 13.65 min Tilt = 10.00 Mean = 99 Exp = 1.732 X/Y/Z = 0.184 0.314 2.262 Errors in X/Y Prediction -0.013 -0.031 um, 1.0 2.4 % Error in Z Prediction 0.020 Predicted X = 0.198 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = 0.336 StdErr = 0.013 nFit = 5 nDrop = 0 Predicted Z = 4.115 StdErr = 0.019 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.007, -0.025 um; position 0.205, 0.361 Drift reached 0.5 A/sec after 5.4 sec, going on Running script # 14 -0.1 1.0 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.70211 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_021_-32.0_Oct22_16.17.05.tif Record image shifted -0.001, -0.002 um Tracking reference shifted by -4.2,4.5 pixels, -0.001,-0.002 um Saved Z = 21 at 14.23 min Tilt = -32.00 Mean = 98 Exp = 1.866 X/Y/Z = 0.207 0.363 4.115 Errors in X/Y Prediction 0.009 0.027 um, 0.7 2.1 % Predicted X = 0.218 StdErr = 0.035 nFit = 4 nDrop = 0 Predicted Y = 0.277 StdErr = 0.016 nFit = 6 nDrop = 0 Predicted Z = 4.190 StdErr = 0.022 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.015, 0.004 um; position 0.204, 0.273 Drift reached 1.0 A/sec after 5.6 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.004 um; position 0.204, 0.269 Running script # 14 0.8 0.5 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.63688 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_022_-34.0_Oct22_16.17.50.tif Record image shifted 0.001, -0.001 um Tracking reference shifted by -2.6,-3.7 pixels, 0.001,-0.001 um Saved Z = 22 at 14.97 min Tilt = -34.00 Mean = 98 Exp = 1.902 X/Y/Z = 0.203 0.270 4.206 Errors in X/Y Prediction -0.015 -0.007 um, 1.2 0.6 % Error in Z Prediction 0.016 Predicted X = 0.200 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Y = 0.277 StdErr = 0.062 nFit = 5 nDrop = 0 Predicted Z = 2.151 StdErr = 0.012 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, -0.070 um; position 0.204, 0.347 Elapsed time 5.4 sec: drift 15.2 A/sec Drift reached 11.3 A/sec after 8.5 sec, going on Running script # 14 -14.5 5.3 unbinned pixels; 3.5 4.2 nm along two shift axes; -1.4 -5.3 nm on specimen axes Rate = 27.08664 A/sec -9.7 2.5 unbinned pixels; 2.0 2.9 nm along two shift axes; -0.6 -3.5 nm on specimen axes Rate = 17.54102 A/sec -6.5 2.3 unbinned pixels; 1.5 1.9 nm along two shift axes; -0.6 -2.3 nm on specimen axes Rate = 12.02891 A/sec Drift is low enough after shot 3 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_023_12.0_Oct22_16.18.41.tif Record image shifted -0.004, -0.026 um Tracking reference shifted by -72.0,17.0 pixels, -0.004,-0.026 um Saved Z = 23 at 15.82 min Tilt = 12.00 Mean = 99 Exp = 1.749 X/Y/Z = 0.214 0.399 2.151 Errors in X/Y Prediction 0.014 0.122 um, 1.1 9.4 % Predicted X = 0.228 StdErr = 0.045 nFit = 4 nDrop = 0 Predicted Y = 0.462 StdErr = 0.072 nFit = 5 nDrop = 0 Predicted Z = 2.046 StdErr = 0.013 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.025, 0.150 um; position 0.204, 0.312 Drift reached 5.8 A/sec after 5.4 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.33 nm/sec Tracking image shifted -0.000, 0.011 um; position 0.205, 0.298 Running script # 14 4.3 2.2 unbinned pixels; 0.2 1.7 nm along two shift axes; -0.9 1.5 nm on specimen axes Rate = 8.54172 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_024_14.0_Oct22_16.19.26.tif Record image shifted 0.003, 0.027 um Tracking reference shifted by 77.0,-14.4 pixels, 0.003,0.027 um Saved Z = 24 at 16.57 min Tilt = 14.00 Mean = 99 Exp = 1.762 X/Y/Z = 0.204 0.270 2.032 Errors in X/Y Prediction -0.025 -0.193 um, 1.9 14.8 % Error in Z Prediction -0.014 Predicted X = 0.218 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Y = 0.175 StdErr = 0.022 nFit = 5 nDrop = 0 Predicted Z = 4.279 StdErr = 0.015 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, -0.025 um; position 0.215, 0.200 Drift reached 1.2 A/sec after 5.6 sec, going on Running script # 14 0.4 1.3 unbinned pixels; 0.4 0.3 nm along two shift axes; -0.5 0.1 nm on specimen axes Rate = 2.39232 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_025_-36.0_Oct22_16.20.02.tif Record image shifted -0.001, -0.002 um Tracking reference shifted by -5.9,4.2 pixels, -0.001,-0.002 um Saved Z = 25 at 17.17 min Tilt = -36.00 Mean = 98 Exp = 1.942 X/Y/Z = 0.217 0.201 4.279 Errors in X/Y Prediction -0.000 0.026 um, 0.0 2.0 % Predicted X = 0.219 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.110 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 4.356 StdErr = 0.016 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.002 um; position 0.214, 0.112 Drift reached 1.1 A/sec after 5.6 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.11 nm/sec Tracking image shifted -0.001, 0.005 um; position 0.214, 0.106 Running script # 14 1.1 0.8 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.4 nm on specimen axes Rate = 2.32945 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_026_-38.0_Oct22_16.20.46.tif Record image shifted 0.001, 0.000 um Tracking reference shifted by -0.2,-3.6 pixels, 0.001,0.000 um Saved Z = 26 at 17.90 min Tilt = -38.00 Mean = 97 Exp = 1.980 X/Y/Z = 0.214 0.106 4.382 Errors in X/Y Prediction -0.006 -0.004 um, 0.5 0.3 % Error in Z Prediction 0.026 Predicted X = 0.220 StdErr = 0.030 nFit = 3 nDrop = 0 Predicted Y = 0.217 StdErr = 0.132 nFit = 5 nDrop = 0 Predicted Z = 1.931 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, -0.059 um; position 0.213, 0.276 Drift reached 13.1 A/sec after 5.4 sec, going on Running script # 14 -13.9 6.1 unbinned pixels; 3.6 3.9 nm along two shift axes; -1.7 -5.0 nm on specimen axes Rate = 26.50061 A/sec -7.9 3.9 unbinned pixels; 2.2 2.2 nm along two shift axes; -1.1 -2.9 nm on specimen axes Rate = 15.53327 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_027_16.0_Oct22_16.21.30.tif Record image shifted -0.005, -0.031 um Tracking reference shifted by -87.3,22.0 pixels, -0.005,-0.031 um Saved Z = 27 at 18.63 min Tilt = 16.00 Mean = 99 Exp = 1.785 X/Y/Z = 0.223 0.322 1.931 Errors in X/Y Prediction 0.002 0.106 um, 0.2 8.1 % Predicted X = 0.219 StdErr = 0.045 nFit = 4 nDrop = 0 Predicted Y = 0.272 StdErr = 0.107 nFit = 6 nDrop = 0 Predicted Z = 1.824 StdErr = 0.024 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, 0.074 um; position 0.206, 0.198 Drift reached 4.2 A/sec after 5.4 sec, going on Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.14 nm/sec Tracking image shifted -0.004, 0.005 um; position 0.211, 0.191 Running script # 14 -0.3 2.5 unbinned pixels; 0.9 0.2 nm along two shift axes; -0.9 -0.2 nm on specimen axes Rate = 4.41019 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_028_18.0_Oct22_16.22.15.tif Record image shifted 0.006, 0.027 um Tracking reference shifted by 76.3,-22.5 pixels, 0.006,0.027 um Saved Z = 28 at 19.39 min Tilt = 18.00 Mean = 99 Exp = 1.803 X/Y/Z = 0.206 0.164 1.884 Errors in X/Y Prediction -0.013 -0.108 um, 1.0 8.2 % Error in Z Prediction 0.060 Predicted X = 0.222 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.004 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 4.464 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, -0.047 um; position 0.224, 0.051 Drift reached 1.3 A/sec after 5.7 sec, going on Running script # 14 0.9 1.0 unbinned pixels; 0.2 0.4 nm along two shift axes; -0.4 0.3 nm on specimen axes Rate = 2.4613 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_029_-40.0_Oct22_16.22.51.tif Record image shifted -0.002, -0.001 um Tracking reference shifted by -3.2,5.7 pixels, -0.002,-0.001 um Saved Z = 29 at 20.01 min Tilt = -40.00 Mean = 97 Exp = 2.032 X/Y/Z = 0.226 0.052 4.464 Errors in X/Y Prediction 0.005 0.047 um, 0.3 3.6 % Predicted X = 0.228 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.012 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 4.548 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, 0.024 um; position 0.225, -0.036 Drift reached 0.8 A/sec after 5.8 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.07 nm/sec Tracking image shifted -0.000, 0.004 um; position 0.225, -0.041 Running script # 14 1.0 0.3 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.82637 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_030_-42.0_Oct22_16.23.37.tif Record image shifted 0.002, -0.002 um Tracking reference shifted by -6.6,-4.4 pixels, 0.002,-0.002 um Saved Z = 30 at 20.77 min Tilt = -42.00 Mean = 97 Exp = 2.084 X/Y/Z = 0.224 -0.039 4.546 Errors in X/Y Prediction -0.005 -0.027 um, 0.4 2.1 % Error in Z Prediction -0.002 Predicted X = 0.231 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.115 StdErr = 0.030 nFit = 5 nDrop = 0 Predicted Z = 4.633 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.012, 0.045 um; position 0.219, -0.160 Drift reached 1.1 A/sec after 5.8 sec, going on Running script # 14 -0.2 1.3 unbinned pixels; 0.4 0.1 nm along two shift axes; -0.4 -0.1 nm on specimen axes Rate = 2.22554 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_031_-44.0_Oct22_16.24.10.tif Record image shifted 0.001, -0.002 um Tracking reference shifted by -4.8,-2.2 pixels, 0.001,-0.002 um Saved Z = 31 at 21.31 min Tilt = -44.00 Mean = 97 Exp = 2.148 X/Y/Z = 0.220 -0.159 4.633 Errors in X/Y Prediction -0.012 -0.044 um, 0.9 3.4 % Predicted X = 0.203 StdErr = 0.042 nFit = 4 nDrop = 0 Predicted Y = 0.029 StdErr = 0.158 nFit = 5 nDrop = 0 Predicted Z = 1.768 StdErr = 0.036 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.010, -0.124 um; position 0.212, 0.153 Drift reached 7.2 A/sec after 5.4 sec, going on Running script # 14 -7.9 4.6 unbinned pixels; 2.4 2.1 nm along two shift axes; -1.4 -2.9 nm on specimen axes Rate = 15.94432 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_032_20.0_Oct22_16.24.48.tif Record image shifted -0.004, -0.012 um Tracking reference shifted by -33.0,15.6 pixels, -0.004,-0.012 um Saved Z = 32 at 21.94 min Tilt = 20.00 Mean = 98 Exp = 1.827 X/Y/Z = 0.220 0.171 1.768 Errors in X/Y Prediction 0.018 0.143 um, 1.4 10.9 % Predicted X = 0.214 StdErr = 0.023 nFit = 3 nDrop = 0 Predicted Y = 0.052 StdErr = 0.133 nFit = 6 nDrop = 0 Predicted Z = 1.670 StdErr = 0.040 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.013 um; position 0.213, 0.039 Drift reached 1.5 A/sec after 5.4 sec, going on Measured defocus = -4.60 microns changed by 0.10 to target drift = 0.11 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.10 nm/sec Tracking image shifted -0.009, 0.002 um; position 0.223, 0.038 Running script # 14 -0.3 2.6 unbinned pixels; 0.9 0.2 nm along two shift axes; -0.9 -0.2 nm on specimen axes Rate = 4.65056 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_033_22.0_Oct22_16.25.47.tif Record image shifted 0.005, 0.013 um Tracking reference shifted by 35.9,-16.9 pixels, 0.005,0.013 um Saved Z = 33 at 22.92 min Tilt = 22.00 Mean = 98 Exp = 1.856 X/Y/Z = 0.219 0.025 1.775 Errors in X/Y Prediction 0.005 -0.028 um, 0.4 2.1 % Error in Z Prediction 0.105 Predicted X = 0.228 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.052 StdErr = 0.121 nFit = 6 nDrop = 0 Predicted Z = 1.666 StdErr = 0.062 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.019, -0.049 um; position 0.209, -0.003 Drift reached 7.8 A/sec after 5.4 sec, going on Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.33 nm/sec Tracking image shifted -0.007, -0.019 um; position 0.218, 0.020 Running script # 14 -4.3 1.8 unbinned pixels; 1.1 1.2 nm along two shift axes; -0.5 -1.6 nm on specimen axes Rate = 8.09204 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_034_24.0_Oct22_16.26.32.tif Record image shifted -0.000, -0.001 um Tracking reference shifted by -3.9,1.7 pixels, -0.000,-0.001 um Saved Z = 34 at 23.68 min Tilt = 24.00 Mean = 98 Exp = 1.892 X/Y/Z = 0.219 0.023 1.722 Errors in X/Y Prediction -0.009 0.075 um, 0.7 5.8 % Error in Z Prediction 0.056 Predicted X = 0.203 StdErr = 0.010 nFit = 4 nDrop = 0 Predicted Y = -0.269 StdErr = 0.040 nFit = 5 nDrop = 0 Predicted Z = 4.718 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.032, -0.033 um; position 0.236, -0.236 Drift reached 2.0 A/sec after 5.8 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.10 nm/sec Tracking image shifted -0.003, 0.004 um; position 0.239, -0.241 Running script # 14 1.0 0.8 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.25667 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_035_-46.0_Oct22_16.27.22.tif Record image shifted -0.001, -0.001 um Tracking reference shifted by -3.5,4.1 pixels, -0.001,-0.001 um Saved Z = 35 at 24.52 min Tilt = -46.00 Mean = 96 Exp = 2.219 X/Y/Z = 0.241 -0.240 4.699 Errors in X/Y Prediction 0.037 0.030 um, 2.9 2.3 % Error in Z Prediction -0.018 Predicted X = 0.245 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = -0.367 StdErr = 0.041 nFit = 5 nDrop = 0 Predicted Z = 4.780 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, -0.027 um; position 0.250, -0.341 Drift reached 1.3 A/sec after 6.0 sec, going on Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.06 nm/sec Tracking image shifted -0.001, 0.001 um; position 0.252, -0.343 Running script # 14 0.4 0.6 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.25789 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_036_-48.0_Oct22_16.28.09.tif Record image shifted 0.000, -0.002 um Tracking reference shifted by -6.5,-0.5 pixels, 0.000,-0.002 um Saved Z = 36 at 25.29 min Tilt = -48.00 Mean = 96 Exp = 2.303 X/Y/Z = 0.252 -0.341 4.719 Errors in X/Y Prediction 0.007 0.027 um, 0.5 2.1 % Error in Z Prediction -0.062 Predicted X = 0.270 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.432 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 4.814 StdErr = 0.039 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.018, -0.046 um; position 0.252, -0.385 Drift reached 1.6 A/sec after 6.2 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.08 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.253, -0.389 Running script # 14 0.9 0.3 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.61599 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_037_-50.0_Oct22_16.28.56.tif Record image shifted 0.000, -0.001 um Tracking reference shifted by -3.6,-0.5 pixels, 0.000,-0.001 um Saved Z = 37 at 26.08 min Tilt = -50.00 Mean = 96 Exp = 2.398 X/Y/Z = 0.253 -0.388 4.762 Errors in X/Y Prediction -0.017 0.044 um, 1.3 3.3 % Error in Z Prediction -0.052 Predicted X = 0.261 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.435 StdErr = 0.027 nFit = 5 nDrop = 0 Predicted Z = 4.829 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.012, 0.028 um; position 0.249, -0.464 Drift reached 1.0 A/sec after 6.2 sec, going on Running script # 14 0.7 1.9 unbinned pixels; 0.5 0.5 nm along two shift axes; -0.7 0.2 nm on specimen axes Rate = 3.54155 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_038_-52.0_Oct22_16.29.35.tif Record image shifted 0.000, -0.001 um Tracking reference shifted by -1.7,-0.3 pixels, 0.000,-0.001 um Saved Z = 38 at 26.73 min Tilt = -52.00 Mean = 95 Exp = 2.502 X/Y/Z = 0.250 -0.463 4.829 Errors in X/Y Prediction -0.011 -0.028 um, 0.8 2.2 % Predicted X = 0.218 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.000 StdErr = 0.124 nFit = 5 nDrop = 0 Predicted Z = 1.653 StdErr = 0.020 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.013, 0.005 um; position 0.231, -0.005 Drift reached 2.9 A/sec after 5.6 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.19 nm/sec Tracking image shifted -0.005, -0.010 um; position 0.237, 0.006 Running script # 14 -3.3 1.1 unbinned pixels; 0.7 0.9 nm along two shift axes; -0.3 -1.2 nm on specimen axes Rate = 6.00036 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_039_26.0_Oct22_16.30.26.tif Record image shifted 0.001, 0.002 um Tracking reference shifted by 5.6,-2.8 pixels, 0.001,0.002 um Saved Z = 39 at 27.58 min Tilt = 26.00 Mean = 98 Exp = 1.925 X/Y/Z = 0.236 0.006 1.638 Errors in X/Y Prediction 0.018 0.006 um, 1.4 0.4 % Error in Z Prediction -0.015 Predicted X = 0.242 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.014 StdErr = 0.117 nFit = 5 nDrop = 0 Predicted Z = 1.590 StdErr = 0.019 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.030, 0.125 um; position 0.212, -0.139 Drift reached 3.9 A/sec after 5.6 sec, going on Running script # 14 -3.7 4.6 unbinned pixels; 2.0 0.7 nm along two shift axes; -1.5 -1.4 nm on specimen axes Rate = 10.33611 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_040_28.0_Oct22_16.30.59.tif Record image shifted -0.001, 0.002 um Tracking reference shifted by 5.4,3.3 pixels, -0.001,0.002 um Saved Z = 40 at 28.13 min Tilt = 28.00 Mean = 98 Exp = 1.972 X/Y/Z = 0.217 -0.138 1.590 Errors in X/Y Prediction -0.025 -0.124 um, 1.9 9.5 % Predicted X = 0.222 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Y = -0.179 StdErr = 0.144 nFit = 5 nDrop = 0 Predicted Z = 1.530 StdErr = 0.022 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.009 um; position 0.215, -0.169 Drift reached 6.0 A/sec after 5.6 sec, going on Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.28 nm/sec Tracking image shifted -0.007, -0.015 um; position 0.223, -0.151 Running script # 14 -1.4 2.1 unbinned pixels; 0.9 0.2 nm along two shift axes; -0.7 -0.6 nm on specimen axes Rate = 4.46201 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_041_30.0_Oct22_16.31.45.tif Record image shifted 0.001, -0.001 um Tracking reference shifted by -3.2,-2.7 pixels, 0.001,-0.001 um Saved Z = 41 at 28.90 min Tilt = 30.00 Mean = 98 Exp = 2.014 X/Y/Z = 0.223 -0.149 1.572 Errors in X/Y Prediction 0.002 0.030 um, 0.1 2.3 % Error in Z Prediction 0.042 Predicted X = 0.212 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = -0.272 StdErr = 0.107 nFit = 5 nDrop = 0 Predicted Z = 1.509 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, -0.044 um; position 0.215, -0.228 Drift reached 2.0 A/sec after 5.9 sec, going on Running script # 14 -2.8 3.9 unbinned pixels; 1.6 0.4 nm along two shift axes; -1.3 -1.1 nm on specimen axes Rate = 8.3437 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_042_32.0_Oct22_16.32.20.tif Record image shifted -0.004, 0.001 um Tracking reference shifted by 3.7,12.2 pixels, -0.004,0.001 um Saved Z = 42 at 29.47 min Tilt = 32.00 Mean = 97 Exp = 2.064 X/Y/Z = 0.221 -0.227 1.509 Errors in X/Y Prediction 0.009 0.045 um, 0.7 3.4 % Predicted X = 0.250 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.513 StdErr = 0.031 nFit = 5 nDrop = 0 Predicted Z = 4.883 StdErr = 0.044 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.006, 0.035 um; position 0.256, -0.548 Drift reached 2.8 A/sec after 6.4 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.13 nm/sec Tracking image shifted -0.001, 0.007 um; position 0.257, -0.557 Running script # 14 1.4 0.8 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 2.86871 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_043_-54.0_Oct22_16.33.13.tif Record image shifted -0.001, -0.006 um Tracking reference shifted by -16.5,4.4 pixels, -0.001,-0.006 um Saved Z = 43 at 30.45 min Tilt = -54.00 Mean = 95 Exp = 2.624 X/Y/Z = 0.259 -0.552 4.874 Errors in X/Y Prediction 0.009 -0.039 um, 0.7 3.0 % Error in Z Prediction -0.009 Predicted X = 0.260 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.622 StdErr = 0.040 nFit = 5 nDrop = 0 Predicted Z = 4.910 StdErr = 0.027 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, -0.033 um; position 0.253, -0.589 Drift reached 1.8 A/sec after 6.6 sec, going on Running script # 14 1.4 1.0 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.4 0.5 nm on specimen axes Rate = 3.08411 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_044_-56.0_Oct22_16.33.52.tif Record image shifted -0.001, -0.002 um Tracking reference shifted by -5.6,2.6 pixels, -0.001,-0.002 um Saved Z = 44 at 31.03 min Tilt = -56.00 Mean = 94 Exp = 2.770 X/Y/Z = 0.254 -0.589 4.910 Errors in X/Y Prediction -0.005 0.034 um, 0.4 2.6 % Predicted X = 0.259 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.660 StdErr = 0.043 nFit = 5 nDrop = 0 Predicted Z = 4.955 StdErr = 0.031 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.002 um; position 0.258, -0.658 Drift reached 0.5 A/sec after 6.8 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.259, -0.661 Running script # 14 0.5 0.9 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.79079 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_045_-58.0_Oct22_16.34.42.tif Record image shifted 0.001, -0.003 um Tracking reference shifted by -7.7,-3.0 pixels, 0.001,-0.003 um Saved Z = 45 at 31.86 min Tilt = -58.00 Mean = 94 Exp = 2.940 X/Y/Z = 0.258 -0.659 4.945 Errors in X/Y Prediction -0.000 0.001 um, 0.0 0.1 % Error in Z Prediction -0.010 Predicted X = 0.256 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.699 StdErr = 0.032 nFit = 5 nDrop = 0 Predicted Z = 4.999 StdErr = 0.018 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.050 um; position 0.257, -0.749 Drift reached 1.1 A/sec after 7.0 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.09 nm/sec Tracking image shifted -0.001, 0.002 um; position 0.258, -0.752 Running script # 14 0.5 0.7 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.47895 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_046_-60.0_Oct22_16.35.33.tif Record image shifted -0.001, -0.005 um Tracking reference shifted by -14.2,2.7 pixels, -0.001,-0.005 um Saved Z = 46 at 32.71 min Tilt = -60.00 Mean = 93 Exp = 3.144 X/Y/Z = 0.259 -0.747 4.973 Errors in X/Y Prediction 0.003 -0.048 um, 0.2 3.7 % Error in Z Prediction -0.026 Predicted X = 0.262 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.821 StdErr = 0.042 nFit = 5 nDrop = 0 Predicted Z = 5.025 StdErr = 0.023 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.022 um; position 0.257, -0.842 Drift reached 0.4 A/sec after 7.4 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.08 nm/sec Tracking image shifted -0.000, 0.005 um; position 0.257, -0.847 Running script # 14 1.0 0.7 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.10776 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_047_-62.0_Oct22_16.36.26.tif Record image shifted 0.000, -0.005 um Tracking reference shifted by -13.1,0.3 pixels, 0.000,-0.005 um Saved Z = 47 at 33.59 min Tilt = -62.00 Mean = 92 Exp = 3.397 X/Y/Z = 0.257 -0.843 4.996 Errors in X/Y Prediction -0.005 -0.023 um, 0.4 1.7 % Error in Z Prediction -0.028 Predicted X = 0.225 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.288 StdErr = 0.096 nFit = 5 nDrop = 0 Predicted Z = 1.458 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.024, -0.108 um; position 0.249, -0.180 Drift reached 2.8 A/sec after 5.8 sec, going on Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.20 nm/sec Tracking image shifted -0.004, -0.005 um; position 0.255, -0.175 Running script # 14 -3.2 1.5 unbinned pixels; 0.9 0.9 nm along two shift axes; -0.4 -1.2 nm on specimen axes Rate = 6.17467 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_048_34.0_Oct22_16.37.20.tif Record image shifted 0.005, 0.005 um Tracking reference shifted by 14.0,-14.6 pixels, 0.005,0.005 um Saved Z = 48 at 34.47 min Tilt = 34.00 Mean = 97 Exp = 2.126 X/Y/Z = 0.251 -0.179 1.367 Errors in X/Y Prediction 0.026 0.109 um, 2.0 8.4 % Error in Z Prediction -0.090 Predicted X = 0.259 StdErr = 0.023 nFit = 3 nDrop = 0 Predicted Y = -0.117 StdErr = 0.078 nFit = 5 nDrop = 0 Predicted Z = 1.325 StdErr = 0.060 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.038, 0.147 um; position 0.221, -0.264 Drift reached 3.5 A/sec after 5.8 sec, going on Measured defocus = -4.61 microns changed by 0.11 to target drift = 0.32 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.13 nm/sec Tracking image shifted -0.005, -0.003 um; position 0.229, -0.260 Running script # 14 -1.0 2.0 unbinned pixels; 0.8 0.1 nm along two shift axes; -0.7 -0.4 nm on specimen axes Rate = 3.86425 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_049_36.0_Oct22_16.38.21.tif Record image shifted -0.001, 0.003 um Tracking reference shifted by 7.8,1.0 pixels, -0.001,0.003 um Saved Z = 49 at 35.50 min Tilt = 36.00 Mean = 97 Exp = 2.189 X/Y/Z = 0.230 -0.262 1.373 Errors in X/Y Prediction -0.030 -0.145 um, 2.3 11.1 % Error in Z Prediction 0.048 Predicted X = 0.242 StdErr = 0.037 nFit = 3 nDrop = 0 Predicted Y = -0.283 StdErr = 0.096 nFit = 5 nDrop = 0 Predicted Z = 1.292 StdErr = 0.064 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.012 um; position 0.237, -0.295 Drift reached 4.8 A/sec after 6.1 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.24 nm/sec Tracking image shifted -0.007, -0.013 um; position 0.245, -0.279 Running script # 14 -3.0 1.5 unbinned pixels; 0.9 0.8 nm along two shift axes; -0.4 -1.1 nm on specimen axes Rate = 5.87199 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_050_38.0_Oct22_16.39.10.tif Record image shifted 0.000, -0.001 um Tracking reference shifted by -2.2,-0.7 pixels, 0.000,-0.001 um Saved Z = 50 at 36.39 min Tilt = 38.00 Mean = 96 Exp = 2.267 X/Y/Z = 0.245 -0.278 1.240 Errors in X/Y Prediction 0.003 0.006 um, 0.2 0.4 % Error in Z Prediction -0.052 Predicted X = 0.237 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Y = -0.310 StdErr = 0.096 nFit = 5 nDrop = 0 Predicted Z = 1.175 StdErr = 0.062 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.050 um; position 0.237, -0.360 Drift reached 3.0 A/sec after 6.0 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.15 nm/sec Tracking image shifted -0.004, -0.007 um; position 0.242, -0.352 Running script # 14 -1.0 1.9 unbinned pixels; 0.7 0.1 nm along two shift axes; -0.6 -0.4 nm on specimen axes Rate = 3.71825 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_051_40.0_Oct22_16.40.03.tif Record image shifted -0.000, 0.002 um Tracking reference shifted by 6.9,0.2 pixels, -0.000,0.002 um Saved Z = 51 at 37.20 min Tilt = 40.00 Mean = 96 Exp = 2.358 X/Y/Z = 0.243 -0.354 1.185 Errors in X/Y Prediction 0.006 -0.044 um, 0.4 3.4 % Error in Z Prediction 0.011 Predicted X = 0.252 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = -0.456 StdErr = 0.075 nFit = 5 nDrop = 0 Predicted Z = 1.104 StdErr = 0.049 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, -0.015 um; position 0.244, -0.441 Drift reached 4.2 A/sec after 6.2 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.24 nm/sec Tracking image shifted -0.005, -0.012 um; position 0.249, -0.426 Running script # 14 -1.7 1.5 unbinned pixels; 0.7 0.4 nm along two shift axes; -0.5 -0.6 nm on specimen axes Rate = 4.01678 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_052_42.0_Oct22_16.40.52.tif Record image shifted 0.001, -0.001 um Tracking reference shifted by -3.4,-2.4 pixels, 0.001,-0.001 um Saved Z = 52 at 38.01 min Tilt = 42.00 Mean = 96 Exp = 2.465 X/Y/Z = 0.249 -0.424 1.086 Errors in X/Y Prediction -0.003 0.032 um, 0.2 2.4 % Error in Z Prediction -0.018 Predicted X = 0.252 StdErr = 0.000 nFit = 4 nDrop = 0 Predicted Y = -0.969 StdErr = 0.013 nFit = 5 nDrop = 1 Predicted Z = 5.032 StdErr = 0.009 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.030, -0.012 um; position 0.282, -0.957 Drift reached 2.2 A/sec after 7.6 sec, going on Running script # 14 4.3 0.7 unbinned pixels; 0.3 1.5 nm along two shift axes; -0.4 1.5 nm on specimen axes Rate = 7.71624 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_053_-64.0_Oct22_16.41.36.tif Record image shifted -0.001, 0.002 um Tracking reference shifted by 4.6,2.5 pixels, -0.001,0.002 um Saved Z = 53 at 38.77 min Tilt = -64.00 Mean = 90 Exp = 3.702 X/Y/Z = 0.284 -0.962 5.032 Errors in X/Y Prediction 0.032 0.007 um, 2.5 0.5 % Predicted X = 0.316 StdErr = 0.022 nFit = 4 nDrop = 0 Predicted Y = -1.092 StdErr = 0.005 nFit = 5 nDrop = 1 Predicted Z = 5.063 StdErr = 0.010 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.032, -0.038 um; position 0.285, -1.054 Drift reached 1.9 A/sec after 8.1 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.11 nm/sec Tracking image shifted -0.002, 0.008 um; position 0.287, -1.063 Running script # 14 1.5 0.5 unbinned pixels; 0.0 0.5 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.73242 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_054_-66.0_Oct22_16.42.39.tif Record image shifted 0.002, -0.023 um Tracking reference shifted by -66.6,1.1 pixels, 0.002,-0.023 um Saved Z = 54 at 39.82 min Tilt = -66.00 Mean = 89 Exp = 4.092 X/Y/Z = 0.286 -1.040 5.009 Errors in X/Y Prediction -0.031 0.051 um, 2.4 3.9 % Error in Z Prediction -0.054 Predicted X = 0.249 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.491 StdErr = 0.051 nFit = 5 nDrop = 0 Predicted Z = 0.992 StdErr = 0.036 nFit = 4 nDrop = 1 Need regular tracking - Tracking image shifted -0.006, -0.047 um; position 0.255, -0.445 Drift reached 1.7 A/sec after 6.4 sec, going on Running script # 14 -6.1 2.1 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.2 nm on specimen axes Rate = 11.35928 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_055_44.0_Oct22_16.43.23.tif Record image shifted -0.002, 0.002 um Tracking reference shifted by 7.2,5.1 pixels, -0.002,0.002 um Saved Z = 55 at 40.53 min Tilt = 44.00 Mean = 95 Exp = 2.576 X/Y/Z = 0.258 -0.444 0.992 Errors in X/Y Prediction 0.009 0.047 um, 0.7 3.6 % Predicted X = 0.275 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = -0.507 StdErr = 0.059 nFit = 5 nDrop = 0 Predicted Z = 0.901 StdErr = 0.043 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted 0.053, 0.042 um; position 0.223, -0.550 Drift reached 3.2 A/sec after 6.4 sec, going on Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.13 nm/sec Tracking image shifted -0.003, -0.004 um; position 0.227, -0.544 Running script # 14 Trimming A by 35 20 55 20, reference by 35 20 55 20 Trimming A by 30 20 50 20, reference by 35 20 55 20 -0.8 1.8 unbinned pixels; 0.7 0.0 nm along two shift axes; -0.6 -0.3 nm on specimen axes Rate = 3.42891 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo7_L5G1_056_46.0_Oct22_16.44.13.tif Record image shifted 0.002, 0.006 um Tracking reference shifted by 17.9,-6.9 pixels, 0.002,0.006 um Saved Z = 56 at 41.37 min Tilt = 46.00 Mean = 94 Exp = 2.719 X/Y/Z = 0.226 -0.550 0.967 Errors in X/Y Prediction -0.050 -0.043 um, 3.8 3.3 % Error in Z Prediction 0.066 Predicted X = 0.221 StdErr = 0.031 nFit = 3 nDrop = 0 Predicted Y = -0.606 StdErr = 0.061 nFit = 5 nDrop = 0 Predicted Z = 0.892 StdErr = 0.024 nFit = 4 nDrop = 1 Need regular tracking - Trimming A by 25 25, reference by 25 25 Tracking image shifted -0.018, 0.027 um; position 0.238, -0.633 2020/10/22 16:44 D:\Users\Ben\201022_Jans_cells\SEM\tomo7_L5G1.st 0 57 3838 3710 1623 -10.0 46.0 2.00 REG 41.4 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Measured defocus = -7.07 microns changed by 2.57 to target drift = 0.20 nm/sec Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.001 um; position -0.000, -0.001 Drift reached 0.5 A/sec after 5.2 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.03 nm/sec Tracking image shifted -0.000, 0.001 um; position 0.000, -0.002 Running script # 14 -0.0 0.9 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.0 nm on specimen axes Rate = 1.67228 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_000_-10.0_Oct22_16.59.41.tif Record image shifted -0.019, -0.015 um Tracking reference shifted by -38.0,56.9 pixels, -0.019,-0.015 um Saved Z = 0 at -0.00 min Tilt = -10.00 Mean = 88 Exp = 1.650 X/Y/Z = 0.019 0.013 3.466 Need regular tracking - Need autofocus Tracking image shifted -0.029, -0.230 um; position 0.048, 0.243 Drift reached 1.4 A/sec after 5.1 sec, going on Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.08 nm/sec Tracking image shifted -0.004, -0.003 um; position 0.053, 0.247 Running script # 14 -0.8 0.5 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.2 -0.3 nm on specimen axes Rate = 1.69626 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_001_-12.0_Oct22_17.00.22.tif Record image shifted -0.000, 0.009 um Tracking reference shifted by 24.8,-1.4 pixels, -0.000,0.009 um Saved Z = 1 at 0.69 min Tilt = -12.00 Mean = 90 Exp = 1.687 X/Y/Z = 0.054 0.238 3.552 Predicted X = 0.088 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.463 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.638 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.028, 0.115 um; position 0.059, 0.348 Drift reached 1.7 A/sec after 5.2 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.05 nm/sec Tracking image shifted 0.002, -0.001 um; position 0.058, 0.350 Running script # 14 -0.2 0.6 unbinned pixels; 0.2 0.0 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.1738 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_002_-14.0_Oct22_17.01.04.tif Record image shifted 0.000, 0.011 um Tracking reference shifted by 30.8,-3.4 pixels, 0.000,0.011 um Saved Z = 2 at 1.39 min Tilt = -14.00 Mean = 88 Exp = 1.696 X/Y/Z = 0.058 0.339 3.617 Errors in X/Y Prediction -0.030 -0.125 um, 2.3 9.5 % Error in Z Prediction -0.022 Need regular tracking - Need autofocus Tracking image shifted -0.057, 0.016 um; position 0.076, -0.003 Drift reached 3.4 A/sec after 5.2 sec, going on Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.14 nm/sec Tracking image shifted -0.001, 0.006 um; position 0.077, -0.010 Running script # 14 1.3 0.2 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.1 0.4 nm on specimen axes Rate = 2.287 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_003_-8.0_Oct22_17.01.55.tif Record image shifted 0.000, -0.006 um Tracking reference shifted by -17.3,0.4 pixels, 0.000,-0.006 um Saved Z = 3 at 2.31 min Tilt = -8.00 Mean = 91 Exp = 1.687 X/Y/Z = 0.077 -0.005 3.361 Need regular tracking - Need autofocus Tracking image shifted -0.003, 0.061 um; position 0.080, -0.065 Drift reached 1.0 A/sec after 5.3 sec, going on Measured defocus = -4.39 microns changed by -0.11 to target drift = 0.07 nm/sec Tracking image shifted 0.001, -0.004 um; position 0.080, -0.061 Running script # 14 -0.8 0.3 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.1 -0.3 nm on specimen axes Rate = 1.49444 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_004_-6.0_Oct22_17.02.43.tif Record image shifted 0.000, -0.011 um Tracking reference shifted by -30.1,2.0 pixels, 0.000,-0.011 um Saved Z = 4 at 3.04 min Tilt = -6.00 Mean = 91 Exp = 1.664 X/Y/Z = 0.080 -0.051 3.250 Predicted X = 0.082 StdErr = 0.022 nFit = 3 nDrop = 0 Predicted Y = 0.522 StdErr = 0.093 nFit = 3 nDrop = 0 Predicted Z = 3.695 StdErr = 0.016 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.015, 0.165 um; position 0.097, 0.357 Drift reached 0.9 A/sec after 5.1 sec, going on Running script # 14 0.1 0.9 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 0.0 nm on specimen axes Rate = 1.67933 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_005_-16.0_Oct22_17.03.16.tif Record image shifted 0.003, 0.011 um Tracking reference shifted by 31.2,-10.6 pixels, 0.003,0.011 um Saved Z = 5 at 3.58 min Tilt = -16.00 Mean = 89 Exp = 1.725 X/Y/Z = 0.095 0.345 3.695 Errors in X/Y Prediction 0.013 -0.177 um, 1.0 13.5 % Predicted X = 0.111 StdErr = 0.025 nFit = 3 nDrop = 0 Predicted Y = 0.415 StdErr = 0.070 nFit = 3 nDrop = 0 Predicted Z = 3.770 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.024, 0.065 um; position 0.087, 0.349 Drift reached 1.9 A/sec after 5.4 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.07 nm/sec Tracking image shifted 0.002, 0.002 um; position 0.085, 0.346 Running script # 14 0.6 0.0 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.0 0.2 nm on specimen axes Rate = 1.05518 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_006_-18.0_Oct22_17.03.58.tif Record image shifted -0.002, 0.013 um Tracking reference shifted by 38.7,3.6 pixels, -0.002,0.013 um Saved Z = 6 at 4.30 min Tilt = -18.00 Mean = 89 Exp = 1.753 X/Y/Z = 0.088 0.333 3.723 Errors in X/Y Prediction -0.023 -0.082 um, 1.7 6.3 % Error in Z Prediction -0.047 Predicted X = 0.082 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.096 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.138 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.009, -0.063 um; position 0.091, -0.033 Drift reached 2.6 A/sec after 5.2 sec, going on Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.10 nm/sec Tracking image shifted -0.001, 0.004 um; position 0.092, -0.039 Running script # 14 0.9 0.3 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.62036 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_007_-4.0_Oct22_17.04.44.tif Record image shifted 0.000, -0.006 um Tracking reference shifted by -16.4,0.3 pixels, 0.000,-0.006 um Saved Z = 7 at 5.06 min Tilt = -4.00 Mean = 91 Exp = 1.652 X/Y/Z = 0.092 -0.033 3.182 Errors in X/Y Prediction 0.010 0.063 um, 0.7 4.8 % Error in Z Prediction 0.044 Predicted X = 0.098 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.058 StdErr = 0.047 nFit = 3 nDrop = 0 Predicted Z = 3.086 StdErr = 0.033 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, -0.044 um; position 0.091, -0.014 Drift reached 1.0 A/sec after 5.2 sec, going on Running script # 14 -2.5 0.5 unbinned pixels; 0.5 0.8 nm along two shift axes; -0.1 -0.9 nm on specimen axes Rate = 4.43893 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_008_-2.0_Oct22_17.05.18.tif Record image shifted -0.001, -0.013 um Tracking reference shifted by -35.4,5.1 pixels, -0.001,-0.013 um Saved Z = 8 at 5.61 min Tilt = -2.00 Mean = 90 Exp = 1.636 X/Y/Z = 0.092 0.000 3.086 Errors in X/Y Prediction -0.006 0.058 um, 0.5 4.4 % Predicted X = 0.110 StdErr = 0.033 nFit = 3 nDrop = 0 Predicted Y = 0.333 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Z = 3.781 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, -0.047 um; position 0.107, 0.380 Drift reached 0.4 A/sec after 5.4 sec, going on Running script # 14 -0.9 0.5 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.1 -0.3 nm on specimen axes Rate = 1.74102 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_009_-20.0_Oct22_17.05.51.tif Record image shifted -0.000, 0.010 um Tracking reference shifted by 28.8,-1.4 pixels, -0.000,0.010 um Saved Z = 9 at 6.18 min Tilt = -20.00 Mean = 88 Exp = 1.781 X/Y/Z = 0.108 0.370 3.781 Errors in X/Y Prediction -0.003 0.037 um, 0.2 2.8 % Predicted X = 0.109 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.404 StdErr = 0.046 nFit = 4 nDrop = 0 Predicted Z = 3.838 StdErr = 0.012 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, 0.062 um; position 0.102, 0.343 Drift reached 2.2 A/sec after 5.4 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Tracking image shifted 0.002, 0.003 um; position 0.101, 0.339 Running script # 14 0.8 0.1 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.49594 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_010_-22.0_Oct22_17.06.34.tif Record image shifted 0.001, 0.017 um Tracking reference shifted by 46.7,-5.9 pixels, 0.001,0.017 um Saved Z = 10 at 6.89 min Tilt = -22.00 Mean = 88 Exp = 1.824 X/Y/Z = 0.101 0.322 3.830 Errors in X/Y Prediction -0.009 -0.083 um, 0.7 6.3 % Error in Z Prediction -0.007 Predicted X = 0.100 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = 0.023 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Z = 2.996 StdErr = 0.044 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, 0.003 um; position 0.107, 0.021 Drift reached 1.5 A/sec after 5.3 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.108, 0.017 Running script # 14 0.5 0.5 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.29372 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_011_-0.0_Oct22_17.07.21.tif Record image shifted 0.001, -0.001 um Tracking reference shifted by -3.8,-2.0 pixels, 0.001,-0.001 um Saved Z = 11 at 7.67 min Tilt = -0.00 Mean = 91 Exp = 1.631 X/Y/Z = 0.107 0.018 2.986 Errors in X/Y Prediction 0.007 -0.005 um, 0.5 0.4 % Error in Z Prediction -0.010 Predicted X = 0.112 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = 0.046 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Z = 2.901 StdErr = 0.028 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.015, 0.083 um; position 0.097, -0.037 Drift reached 1.3 A/sec after 5.2 sec, going on Running script # 14 0.2 1.7 unbinned pixels; 0.5 0.3 nm along two shift axes; -0.6 0.0 nm on specimen axes Rate = 3.01934 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_012_2.0_Oct22_17.07.54.tif Record image shifted 0.002, -0.008 um Tracking reference shifted by -23.6,-4.2 pixels, 0.002,-0.008 um Saved Z = 12 at 8.21 min Tilt = 2.00 Mean = 90 Exp = 1.616 X/Y/Z = 0.096 -0.029 2.901 Errors in X/Y Prediction -0.016 -0.075 um, 1.2 5.7 % Predicted X = 0.111 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.309 StdErr = 0.053 nFit = 5 nDrop = 0 Predicted Z = 3.883 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, 0.021 um; position 0.118, 0.288 Drift reached 0.2 A/sec after 5.4 sec, going on Running script # 14 -0.8 0.2 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.0 -0.3 nm on specimen axes Rate = 1.44572 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_013_-24.0_Oct22_17.08.27.tif Record image shifted 0.000, 0.012 um Tracking reference shifted by 34.6,-4.3 pixels, 0.000,0.012 um Saved Z = 13 at 8.78 min Tilt = -24.00 Mean = 88 Exp = 1.863 X/Y/Z = 0.117 0.276 3.883 Errors in X/Y Prediction 0.006 -0.033 um, 0.4 2.5 % Predicted X = 0.118 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.209 StdErr = 0.051 nFit = 5 nDrop = 0 Predicted Z = 3.937 StdErr = 0.000 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, -0.038 um; position 0.111, 0.248 Drift reached 1.9 A/sec after 5.7 sec, going on Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.07 nm/sec Tracking image shifted -0.000, 0.003 um; position 0.112, 0.244 Running script # 14 0.6 0.2 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 1.08617 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_014_-26.0_Oct22_17.09.11.tif Record image shifted -0.000, 0.018 um Tracking reference shifted by 49.8,-3.6 pixels, -0.000,0.018 um Saved Z = 14 at 9.51 min Tilt = -26.00 Mean = 88 Exp = 1.913 X/Y/Z = 0.112 0.226 3.956 Errors in X/Y Prediction -0.006 0.017 um, 0.5 1.3 % Error in Z Prediction 0.019 Predicted X = 0.089 StdErr = 0.028 nFit = 4 nDrop = 0 Predicted Y = -0.103 StdErr = 0.032 nFit = 4 nDrop = 0 Predicted Z = 2.812 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.020, -0.133 um; position 0.109, 0.030 Drift reached 2.0 A/sec after 5.2 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.05 nm/sec Tracking image shifted -0.002, 0.003 um; position 0.112, 0.027 Running script # 14 -0.1 0.8 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.48434 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_015_4.0_Oct22_17.09.59.tif Record image shifted -0.002, -0.003 um Tracking reference shifted by -9.1,7.3 pixels, -0.002,-0.003 um Saved Z = 15 at 10.30 min Tilt = 4.00 Mean = 90 Exp = 1.615 X/Y/Z = 0.115 0.030 2.789 Errors in X/Y Prediction 0.025 0.133 um, 1.9 10.2 % Error in Z Prediction -0.023 Predicted X = 0.121 StdErr = 0.037 nFit = 4 nDrop = 0 Predicted Y = 0.020 StdErr = 0.078 nFit = 5 nDrop = 0 Predicted Z = 2.691 StdErr = 0.001 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.016, 0.018 um; position 0.105, 0.002 Drift reached 0.9 A/sec after 5.3 sec, going on Running script # 14 -1.1 0.6 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.2 -0.4 nm on specimen axes Rate = 2.28781 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_016_6.0_Oct22_17.10.31.tif Record image shifted 0.001, -0.006 um Tracking reference shifted by -16.6,-0.5 pixels, 0.001,-0.006 um Saved Z = 16 at 10.85 min Tilt = 6.00 Mean = 90 Exp = 1.608 X/Y/Z = 0.105 0.008 2.691 Errors in X/Y Prediction -0.016 -0.012 um, 1.2 0.9 % Predicted X = 0.121 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.127 StdErr = 0.046 nFit = 5 nDrop = 0 Predicted Z = 4.011 StdErr = 0.012 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, -0.050 um; position 0.124, 0.177 Drift reached 0.7 A/sec after 5.6 sec, going on Running script # 14 -0.0 0.6 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 -0.0 nm on specimen axes Rate = 1.08065 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_017_-28.0_Oct22_17.11.06.tif Record image shifted -0.001, 0.015 um Tracking reference shifted by 41.9,-2.0 pixels, -0.001,0.015 um Saved Z = 17 at 11.43 min Tilt = -28.00 Mean = 87 Exp = 1.964 X/Y/Z = 0.125 0.162 4.011 Errors in X/Y Prediction 0.004 0.035 um, 0.3 2.7 % Predicted X = 0.126 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.099 StdErr = 0.008 nFit = 5 nDrop = 0 Predicted Z = 4.069 StdErr = 0.014 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.022 um; position 0.120, 0.120 Drift reached 1.8 A/sec after 5.6 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.09 nm/sec Tracking image shifted -0.000, 0.002 um; position 0.121, 0.117 Running script # 14 0.4 0.4 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.01846 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_018_-30.0_Oct22_17.11.55.tif Record image shifted 0.001, 0.019 um Tracking reference shifted by 54.6,-7.9 pixels, 0.001,0.019 um Saved Z = 18 at 12.24 min Tilt = -30.00 Mean = 87 Exp = 2.029 X/Y/Z = 0.120 0.098 4.050 Errors in X/Y Prediction -0.006 -0.001 um, 0.5 0.1 % Error in Z Prediction -0.019 Predicted X = 0.111 StdErr = 0.039 nFit = 4 nDrop = 0 Predicted Y = 0.006 StdErr = 0.055 nFit = 6 nDrop = 0 Predicted Z = 2.592 StdErr = 0.001 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.006, -0.047 um; position 0.117, 0.053 Drift reached 1.1 A/sec after 5.2 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.13 nm/sec Tracking image shifted -0.002, -0.006 um; position 0.119, 0.059 Running script # 14 -2.6 2.3 unbinned pixels; 1.1 0.6 nm along two shift axes; -0.7 -1.0 nm on specimen axes Rate = 6.12721 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_019_8.0_Oct22_17.12.44.tif Record image shifted -0.000, -0.004 um Tracking reference shifted by -10.3,1.3 pixels, -0.000,-0.004 um Saved Z = 19 at 13.05 min Tilt = 8.00 Mean = 90 Exp = 1.604 X/Y/Z = 0.120 0.064 2.542 Errors in X/Y Prediction 0.009 0.058 um, 0.7 4.4 % Error in Z Prediction -0.050 Predicted X = 0.119 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = 0.098 StdErr = 0.054 nFit = 6 nDrop = 0 Predicted Z = 2.439 StdErr = 0.031 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.012, 0.092 um; position 0.107, 0.006 Drift reached 2.3 A/sec after 5.2 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.07 nm/sec Tracking image shifted 0.001, 0.002 um; position 0.106, 0.003 Running script # 14 0.8 0.8 unbinned pixels; 0.2 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.00569 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_020_10.0_Oct22_17.13.28.tif Record image shifted -0.000, -0.001 um Tracking reference shifted by -2.6,1.4 pixels, -0.000,-0.001 um Saved Z = 20 at 13.79 min Tilt = 10.00 Mean = 90 Exp = 1.605 X/Y/Z = 0.107 0.004 2.473 Errors in X/Y Prediction -0.012 -0.094 um, 0.9 7.2 % Error in Z Prediction 0.034 Predicted X = 0.127 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = 0.023 StdErr = 0.005 nFit = 5 nDrop = 0 Predicted Z = 4.110 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, -0.005 um; position 0.128, 0.028 Drift reached 0.3 A/sec after 5.8 sec, going on Running script # 14 -0.8 1.3 unbinned pixels; 0.5 0.1 nm along two shift axes; -0.4 -0.3 nm on specimen axes Rate = 2.68491 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_021_-32.0_Oct22_17.14.09.tif Record image shifted -0.001, 0.018 um Tracking reference shifted by 50.3,-1.7 pixels, -0.001,0.018 um Saved Z = 21 at 14.48 min Tilt = -32.00 Mean = 87 Exp = 2.095 X/Y/Z = 0.130 0.010 4.110 Errors in X/Y Prediction 0.003 -0.012 um, 0.2 0.9 % Predicted X = 0.130 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -0.080 StdErr = 0.009 nFit = 5 nDrop = 0 Predicted Z = 4.165 StdErr = 0.024 nFit = 3 nDrop = 0 Need autofocus Drift reached 1.0 A/sec after 5.3 sec, going on Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.07 nm/sec Running script # 14 0.6 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.68755 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_022_-34.0_Oct22_17.14.50.tif Record image shifted 0.003, -0.033 um Saved Z = 22 at 15.15 min Tilt = -34.00 Mean = 85 Exp = 2.172 X/Y/Z = 0.127 -0.048 4.136 Errors in X/Y Prediction -0.003 0.032 um, 0.2 2.5 % Error in Z Prediction -0.029 Predicted X = 0.106 StdErr = 0.035 nFit = 4 nDrop = 0 Predicted Y = 0.021 StdErr = 0.076 nFit = 6 nDrop = 0 Predicted Z = 2.354 StdErr = 0.029 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.021, -0.006 um; position 0.126, 0.027 Drift reached 10.4 A/sec after 5.2 sec, going on Running script # 14 -16.0 5.1 unbinned pixels; 3.6 4.7 nm along two shift axes; -1.3 -5.8 nm on specimen axes Rate = 29.38771 A/sec -9.9 1.2 unbinned pixels; 1.6 3.1 nm along two shift axes; -0.1 -3.5 nm on specimen axes Rate = 17.49003 A/sec -9.1 4.9 unbinned pixels; 2.7 2.4 nm along two shift axes; -1.4 -3.4 nm on specimen axes Rate = 18.17034 A/sec -6.1 2.4 unbinned pixels; 1.5 1.7 nm along two shift axes; -0.7 -2.2 nm on specimen axes Rate = 11.49211 A/sec Drift is low enough after shot 4 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_023_12.0_Oct22_17.15.43.tif Record image shifted -0.003, -0.020 um Tracking reference shifted by -56.5,12.9 pixels, -0.003,-0.020 um Saved Z = 23 at 16.04 min Tilt = 12.00 Mean = 90 Exp = 1.604 X/Y/Z = 0.134 0.068 2.354 Errors in X/Y Prediction 0.028 0.047 um, 2.1 3.6 % Predicted X = 0.134 StdErr = 0.029 nFit = 3 nDrop = 0 Predicted Y = 0.084 StdErr = 0.090 nFit = 5 nDrop = 0 Predicted Z = 2.248 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, 0.118 um; position 0.126, -0.034 Drift reached 4.9 A/sec after 5.2 sec, going on Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.28 nm/sec Tracking image shifted -0.002, 0.010 um; position 0.129, -0.046 Running script # 14 3.6 2.5 unbinned pixels; 0.4 1.5 nm along two shift axes; -1.0 1.2 nm on specimen axes Rate = 7.82419 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_024_14.0_Oct22_17.16.29.tif Record image shifted 0.002, 0.017 um Tracking reference shifted by 48.9,-10.4 pixels, 0.002,0.017 um Saved Z = 24 at 16.80 min Tilt = 14.00 Mean = 91 Exp = 1.610 X/Y/Z = 0.128 -0.065 2.245 Errors in X/Y Prediction -0.006 -0.149 um, 0.5 11.4 % Error in Z Prediction -0.003 Predicted X = 0.133 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.126 StdErr = 0.021 nFit = 5 nDrop = 0 Predicted Z = 4.182 StdErr = 0.005 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.007, -0.063 um; position 0.139, -0.063 Drift reached 1.6 A/sec after 6.0 sec, going on Running script # 14 -0.1 1.3 unbinned pixels; 0.4 0.1 nm along two shift axes; -0.4 -0.1 nm on specimen axes Rate = 2.25211 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_025_-36.0_Oct22_17.17.10.tif Record image shifted -0.001, 0.042 um Tracking reference shifted by 119.2,-7.9 pixels, -0.001,0.042 um Saved Z = 25 at 17.50 min Tilt = -36.00 Mean = 87 Exp = 2.290 X/Y/Z = 0.141 -0.105 4.182 Errors in X/Y Prediction 0.009 0.021 um, 0.7 1.6 % Predicted X = 0.144 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.159 StdErr = 0.020 nFit = 5 nDrop = 0 Predicted Z = 4.227 StdErr = 0.006 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.010 um; position 0.142, -0.149 Drift reached 1.2 A/sec after 6.0 sec, going on Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.09 nm/sec Tracking image shifted -0.000, 0.004 um; position 0.142, -0.154 Running script # 14 1.1 1.0 unbinned pixels; 0.2 0.5 nm along two shift axes; -0.4 0.4 nm on specimen axes Rate = 2.65707 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_026_-38.0_Oct22_17.17.56.tif Record image shifted 0.002, 0.025 um Tracking reference shifted by 70.3,-11.4 pixels, 0.002,0.025 um Saved Z = 26 at 18.27 min Tilt = -38.00 Mean = 85 Exp = 2.361 X/Y/Z = 0.141 -0.179 4.270 Errors in X/Y Prediction -0.004 -0.020 um, 0.3 1.5 % Error in Z Prediction 0.043 Predicted X = 0.143 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Y = -0.104 StdErr = 0.098 nFit = 6 nDrop = 0 Predicted Z = 2.137 StdErr = 0.031 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.011, -0.082 um; position 0.132, -0.022 Drift reached 12.1 A/sec after 5.2 sec, going on Running script # 14 -14.9 8.2 unbinned pixels; 4.5 3.9 nm along two shift axes; -2.4 -5.5 nm on specimen axes Rate = 29.7563 A/sec -9.5 5.5 unbinned pixels; 2.9 2.5 nm along two shift axes; -1.6 -3.5 nm on specimen axes Rate = 19.18762 A/sec -5.9 3.6 unbinned pixels; 1.9 1.5 nm along two shift axes; -1.1 -2.2 nm on specimen axes Rate = 12.08106 A/sec Drift is low enough after shot 3 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_027_16.0_Oct22_17.18.45.tif Record image shifted -0.004, -0.028 um Tracking reference shifted by -78.7,19.0 pixels, -0.004,-0.028 um Saved Z = 27 at 19.07 min Tilt = 16.00 Mean = 89 Exp = 1.600 X/Y/Z = 0.143 0.024 2.137 Errors in X/Y Prediction 0.000 0.128 um, 0.0 9.8 % Predicted X = 0.145 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.024 StdErr = 0.156 nFit = 5 nDrop = 0 Predicted Z = 2.030 StdErr = 0.035 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, 0.094 um; position 0.135, -0.069 Drift reached 6.5 A/sec after 5.2 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.32 nm/sec Tracking image shifted -0.004, 0.011 um; position 0.141, -0.083 Running script # 14 2.5 2.9 unbinned pixels; 0.7 1.2 nm along two shift axes; -1.1 0.8 nm on specimen axes Rate = 6.85984 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_028_18.0_Oct22_17.19.34.tif Record image shifted 0.003, 0.027 um Tracking reference shifted by 74.3,-15.4 pixels, 0.003,0.027 um Saved Z = 28 at 19.89 min Tilt = 18.00 Mean = 91 Exp = 1.620 X/Y/Z = 0.139 -0.111 2.064 Errors in X/Y Prediction -0.006 -0.135 um, 0.4 10.3 % Error in Z Prediction 0.034 Predicted X = 0.150 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -0.232 StdErr = 0.024 nFit = 5 nDrop = 0 Predicted Z = 4.317 StdErr = 0.031 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, 0.049 um; position 0.156, -0.282 Drift reached 1.7 A/sec after 6.3 sec, going on Running script # 14 Trimming A by 30 20 35 20, reference by 30 20 35 20 Trimming A by 30 20 35 20, reference by 30 20 35 20 1.0 1.6 unbinned pixels; 0.4 0.5 nm along two shift axes; -0.6 0.3 nm on specimen axes Rate = 3.38183 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_029_-40.0_Oct22_17.20.12.tif Record image shifted -0.001, 0.024 um Tracking reference shifted by 68.0,-4.3 pixels, -0.001,0.024 um Saved Z = 29 at 20.54 min Tilt = -40.00 Mean = 85 Exp = 2.489 X/Y/Z = 0.158 -0.307 4.317 Errors in X/Y Prediction 0.008 -0.075 um, 0.6 5.7 % Predicted X = 0.167 StdErr = 0.022 nFit = 4 nDrop = 0 Predicted Y = -0.433 StdErr = 0.029 nFit = 5 nDrop = 1 Predicted Z = 4.372 StdErr = 0.036 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 50 50, reference by 20 20 Tracking image shifted 0.012, -0.060 um; position 0.156, -0.373 Drift reached 1.9 A/sec after 6.4 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.09 nm/sec Trimming A by 35 40, reference by 20 20 Tracking image shifted -0.001, 0.005 um; position 0.157, -0.379 Running script # 14 Trimming A by 45 21 45 20, reference by 45 21 45 20 Trimming A by 45 21 50 20, reference by 45 21 45 20 0.9 0.9 unbinned pixels; 0.2 0.4 nm along two shift axes; -0.4 0.3 nm on specimen axes Rate = 2.32076 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_030_-42.0_Oct22_17.21.01.tif Record image shifted 0.001, 0.028 um Tracking reference shifted by 78.8,-8.8 pixels, 0.001,0.028 um Saved Z = 30 at 21.34 min Tilt = -42.00 Mean = 84 Exp = 2.643 X/Y/Z = 0.156 -0.407 4.343 Errors in X/Y Prediction -0.011 0.026 um, 0.9 2.0 % Error in Z Prediction -0.029 Predicted X = 0.167 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.555 StdErr = 0.033 nFit = 5 nDrop = 0 Predicted Z = 4.403 StdErr = 0.026 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 27 90, reference by 45 45 Tracking image shifted 0.016, -0.089 um; position 0.151, -0.465 Drift reached 1.2 A/sec after 6.6 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.05 nm/sec Trimming A by 55 60, reference by 45 45 Tracking image shifted -0.002, -0.000 um; position 0.154, -0.466 Running script # 14 Trimming A by 46 20 75 20, reference by 46 20 75 20 Trimming A by 60 24 60 20, reference by 46 20 75 20 0.2 0.7 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 0.0 nm on specimen axes Rate = 1.26067 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_031_-44.0_Oct22_17.21.50.tif Record image shifted 0.000, 0.029 um Tracking reference shifted by 80.9,-8.1 pixels, 0.000,0.029 um Saved Z = 31 at 22.17 min Tilt = -44.00 Mean = 83 Exp = 2.821 X/Y/Z = 0.153 -0.494 4.361 Errors in X/Y Prediction -0.014 0.060 um, 1.1 4.6 % Error in Z Prediction -0.042 Predicted X = 0.148 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = -0.186 StdErr = 0.173 nFit = 5 nDrop = 0 Predicted Z = 1.962 StdErr = 0.027 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.006, -0.097 um; position 0.142, -0.088 Drift reached 9.0 A/sec after 5.2 sec, going on Running script # 14 -10.9 6.2 unbinned pixels; 3.3 2.8 nm along two shift axes; -1.9 -4.0 nm on specimen axes Rate = 21.9351 A/sec -8.5 5.0 unbinned pixels; 2.7 2.2 nm along two shift axes; -1.5 -3.1 nm on specimen axes Rate = 17.3117 A/sec -6.1 3.1 unbinned pixels; 1.7 1.6 nm along two shift axes; -0.9 -2.2 nm on specimen axes Rate = 12.01099 A/sec Drift is low enough after shot 3 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_032_20.0_Oct22_17.22.40.tif Record image shifted -0.004, -0.020 um Tracking reference shifted by -55.8,15.8 pixels, -0.004,-0.020 um Saved Z = 32 at 22.98 min Tilt = 20.00 Mean = 89 Exp = 1.606 X/Y/Z = 0.152 -0.054 1.962 Errors in X/Y Prediction 0.004 0.131 um, 0.3 10.0 % Predicted X = 0.154 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.037 StdErr = 0.172 nFit = 5 nDrop = 0 Predicted Z = 1.863 StdErr = 0.030 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, 0.132 um; position 0.147, -0.170 Drift reached 5.8 A/sec after 5.2 sec, going on Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.20 nm/sec Tracking image shifted -0.004, 0.009 um; position 0.153, -0.181 Running script # 14 0.1 2.6 unbinned pixels; 0.8 0.3 nm along two shift axes; -0.9 -0.1 nm on specimen axes Rate = 4.55974 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_033_22.0_Oct22_17.23.24.tif Record image shifted 0.004, 0.017 um Tracking reference shifted by 47.9,-16.8 pixels, 0.004,0.017 um Saved Z = 33 at 23.71 min Tilt = 22.00 Mean = 90 Exp = 1.633 X/Y/Z = 0.149 -0.198 1.926 Errors in X/Y Prediction -0.005 -0.161 um, 0.3 12.3 % Error in Z Prediction 0.063 Predicted X = 0.157 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.321 StdErr = 0.161 nFit = 5 nDrop = 0 Predicted Z = 1.839 StdErr = 0.028 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.010, -0.122 um; position 0.147, -0.200 Drift reached 11.9 A/sec after 5.2 sec, going on Running script # 14 -13.0 7.7 unbinned pixels; 4.1 3.4 nm along two shift axes; -2.3 -4.8 nm on specimen axes Rate = 26.47909 A/sec -8.9 5.4 unbinned pixels; 2.8 2.3 nm along two shift axes; -1.6 -3.3 nm on specimen axes Rate = 18.16189 A/sec -6.0 3.1 unbinned pixels; 1.7 1.6 nm along two shift axes; -0.9 -2.2 nm on specimen axes Rate = 11.8628 A/sec Drift is low enough after shot 3 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_034_24.0_Oct22_17.24.08.tif Record image shifted -0.006, -0.019 um Tracking reference shifted by -50.9,21.6 pixels, -0.006,-0.019 um Saved Z = 34 at 24.44 min Tilt = 24.00 Mean = 88 Exp = 1.627 X/Y/Z = 0.161 -0.165 1.839 Errors in X/Y Prediction 0.003 0.156 um, 0.2 12.0 % Predicted X = 0.152 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.600 StdErr = 0.040 nFit = 5 nDrop = 0 Predicted Z = 4.424 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 105, reference by 60 60 Tracking image shifted -0.016, -0.034 um; position 0.168, -0.566 Drift reached 2.8 A/sec after 6.8 sec, going on Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.13 nm/sec Trimming A by 22 100, reference by 60 60 Tracking image shifted -0.002, 0.007 um; position 0.171, -0.574 Running script # 14 Trimming A by 21 20 100 20, reference by 21 20 100 20 Trimming A by 21 20 100 20, reference by 21 20 100 20 1.8 0.7 unbinned pixels; 0.0 0.7 nm along two shift axes; -0.3 0.6 nm on specimen axes Rate = 3.35788 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_035_-46.0_Oct22_17.25.02.tif Record image shifted 0.000, 0.035 um Tracking reference shifted by 97.8,-9.5 pixels, 0.000,0.035 um Saved Z = 35 at 25.38 min Tilt = -46.00 Mean = 83 Exp = 3.046 X/Y/Z = 0.171 -0.609 4.398 Errors in X/Y Prediction 0.019 -0.009 um, 1.5 0.7 % Error in Z Prediction -0.027 Predicted X = 0.175 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = -0.695 StdErr = 0.029 nFit = 5 nDrop = 0 Predicted Z = 4.429 StdErr = 0.010 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 21 105, reference by 21 100 Tracking image shifted 0.003, -0.053 um; position 0.172, -0.641 Trimming A by 20 20 25 20, reference by 20 20 25 20 Drift reached 0.8 A/sec after 7.2 sec, going on Running script # 14 Trimming A by 24 21 105 20, reference by 24 21 105 20 Trimming A by 24 21 105 20, reference by 24 21 105 20 0.5 0.6 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.44365 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_036_-48.0_Oct22_17.25.40.tif Trimming A by 25 25, reference by 20 20 Record image shifted -0.000, 0.035 um Tracking reference shifted by 98.4,-7.7 pixels, -0.000,0.035 um Saved Z = 36 at 26.01 min Tilt = -48.00 Mean = 82 Exp = 3.323 X/Y/Z = 0.173 -0.677 4.429 Errors in X/Y Prediction -0.002 0.018 um, 0.1 1.4 % Predicted X = 0.186 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.763 StdErr = 0.029 nFit = 5 nDrop = 0 Predicted Z = 4.460 StdErr = 0.012 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 100, reference by 21 100 Tracking image shifted 0.016, -0.067 um; position 0.169, -0.696 Trimming A by 20 20 35 20, reference by 20 20 35 20 Drift reached 1.8 A/sec after 7.6 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.10 nm/sec Trimming A by 21 100, reference by 21 100 Tracking image shifted -0.001, 0.003 um; position 0.170, -0.700 Running script # 14 Trimming A by 23 20 100 20, reference by 23 20 100 20 Trimming A by 22 20 100 30, reference by 23 20 100 20 1.4 0.2 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.1 0.5 nm on specimen axes Rate = 2.54287 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_037_-50.0_Oct22_17.26.35.tif Trimming A by 45 45, reference by 35 35 Record image shifted 0.001, 0.035 um Tracking reference shifted by 97.4,-12.4 pixels, 0.001,0.035 um Saved Z = 37 at 26.94 min Tilt = -50.00 Mean = 81 Exp = 3.658 X/Y/Z = 0.169 -0.735 4.459 Errors in X/Y Prediction -0.016 0.027 um, 1.3 2.1 % Error in Z Prediction -0.001 Predicted X = 0.169 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.784 StdErr = 0.030 nFit = 5 nDrop = 0 Predicted Z = 4.487 StdErr = 0.010 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 30 100, reference by 20 100 Tracking image shifted 0.004, -0.044 um; position 0.165, -0.740 Trimming A by 20 20 35 20, reference by 20 20 35 20 Drift reached 1.3 A/sec after 8.2 sec, going on Running script # 14 Trimming A by 28 20 100 20, reference by 28 20 100 20 Trimming A by 28 20 100 20, reference by 28 20 100 20 1.2 0.5 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.25397 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_038_-52.0_Oct22_17.27.20.tif Trimming A by 60 65, reference by 55 55 Record image shifted -0.001, 0.030 um Tracking reference shifted by 84.6,-5.6 pixels, -0.001,0.030 um Saved Z = 38 at 27.69 min Tilt = -52.00 Mean = 80 Exp = 4.076 X/Y/Z = 0.166 -0.771 4.487 Errors in X/Y Prediction -0.003 0.013 um, 0.2 1.0 % Predicted X = 0.162 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.173 StdErr = 0.154 nFit = 5 nDrop = 0 Predicted Z = 1.759 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.011, -0.002 um; position 0.173, -0.171 Drift reached 7.4 A/sec after 5.2 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.27 nm/sec Tracking image shifted -0.008, -0.018 um; position 0.183, -0.149 Running script # 14 -4.2 3.9 unbinned pixels; 1.8 0.9 nm along two shift axes; -1.2 -1.6 nm on specimen axes Rate = 10.11969 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_039_26.0_Oct22_17.28.12.tif Record image shifted 0.008, 0.020 um Tracking reference shifted by 53.3,-26.3 pixels, 0.008,0.020 um Saved Z = 39 at 28.52 min Tilt = 26.00 Mean = 90 Exp = 1.662 X/Y/Z = 0.177 -0.167 1.778 Errors in X/Y Prediction 0.014 0.006 um, 1.1 0.5 % Error in Z Prediction 0.018 Predicted X = 0.189 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.175 StdErr = 0.147 nFit = 5 nDrop = 0 Predicted Z = 1.708 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.040, 0.112 um; position 0.149, -0.287 Drift reached 4.9 A/sec after 5.2 sec, going on Running script # 14 -0.5 5.4 unbinned pixels; 1.9 0.5 nm along two shift axes; -1.9 -0.3 nm on specimen axes Rate = 9.63764 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_040_28.0_Oct22_17.28.45.tif Record image shifted -0.001, 0.008 um Tracking reference shifted by 22.4,1.7 pixels, -0.001,0.008 um Saved Z = 40 at 29.06 min Tilt = 28.00 Mean = 89 Exp = 1.660 X/Y/Z = 0.155 -0.296 1.708 Errors in X/Y Prediction -0.035 -0.121 um, 2.7 9.2 % Predicted X = 0.158 StdErr = 0.028 nFit = 3 nDrop = 0 Predicted Y = -0.315 StdErr = 0.163 nFit = 5 nDrop = 0 Predicted Z = 1.636 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.030 um; position 0.153, -0.345 Drift reached 6.0 A/sec after 5.2 sec, going on Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.32 nm/sec Tracking image shifted -0.006, -0.015 um; position 0.160, -0.327 Running script # 14 -4.2 1.9 unbinned pixels; 1.1 1.2 nm along two shift axes; -0.6 -1.5 nm on specimen axes Rate = 8.09989 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_041_30.0_Oct22_17.29.29.tif Record image shifted 0.000, -0.004 um Tracking reference shifted by -12.6,0.1 pixels, 0.000,-0.004 um Saved Z = 41 at 29.80 min Tilt = 30.00 Mean = 88 Exp = 1.680 X/Y/Z = 0.160 -0.321 1.714 Errors in X/Y Prediction 0.002 -0.006 um, 0.2 0.5 % Error in Z Prediction 0.078 Predicted X = 0.147 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = -0.464 StdErr = 0.098 nFit = 5 nDrop = 0 Predicted Z = 1.647 StdErr = 0.054 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.090 um; position 0.149, -0.375 Drift reached 3.4 A/sec after 5.2 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.19 nm/sec Tracking image shifted -0.005, -0.005 um; position 0.156, -0.369 Running script # 14 -2.8 3.6 unbinned pixels; 1.5 0.5 nm along two shift axes; -1.2 -1.1 nm on specimen axes Rate = 8.02999 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_042_32.0_Oct22_17.30.18.tif Record image shifted -0.000, 0.004 um Tracking reference shifted by 12.7,-0.8 pixels, -0.000,0.004 um Saved Z = 42 at 30.62 min Tilt = 32.00 Mean = 89 Exp = 1.710 X/Y/Z = 0.157 -0.372 1.671 Errors in X/Y Prediction 0.010 0.092 um, 0.8 7.1 % Error in Z Prediction 0.024 Predicted X = 0.162 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.777 StdErr = 0.017 nFit = 5 nDrop = 0 Predicted Z = 4.516 StdErr = 0.011 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 28 115, reference by 20 100 Tracking image shifted -0.021, 0.014 um; position 0.183, -0.791 Drift reached 1.5 A/sec after 8.9 sec, going on Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.09 nm/sec Trimming A by 23 110, reference by 20 100 Tracking image shifted -0.001, 0.006 um; position 0.185, -0.799 Running script # 14 Trimming A by 43 23 110 20, reference by 43 23 110 20 Trimming A by 44 24 110 20, reference by 43 23 110 20 3.5 0.7 unbinned pixels; 0.2 1.2 nm along two shift axes; -0.4 1.2 nm on specimen axes Rate = 6.19799 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 31 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_043_-54.0_Oct22_17.31.23.tif Trimming A by 85 85, reference by 70 70 Record image shifted 0.001, 0.034 um Tracking reference shifted by 95.0,-11.0 pixels, 0.001,0.034 um Saved Z = 43 at 31.84 min Tilt = -54.00 Mean = 78 Exp = 4.610 X/Y/Z = 0.184 -0.834 4.508 Errors in X/Y Prediction 0.022 -0.057 um, 1.7 4.3 % Error in Z Prediction -0.008 Predicted X = 0.188 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = -0.872 StdErr = 0.023 nFit = 5 nDrop = 0 Predicted Z = 4.541 StdErr = 0.009 nFit = 4 nDrop = 0 Need regular tracking - Trimming A by 20 130, reference by 20 115 Tracking image shifted 0.006, -0.021 um; position 0.183, -0.851 Trimming A by 20 20 55 20, reference by 25 22 55 20 Drift reached 1.3 A/sec after 9.7 sec, going on Running script # 14 Trimming A by 46 20 120 20, reference by 46 20 120 20 Trimming A by 23 24 130 20, reference by 46 20 120 20 1.7 0.4 unbinned pixels; 0.0 0.6 nm along two shift axes; -0.2 0.6 nm on specimen axes Rate = 3.02935 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 36 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_044_-56.0_Oct22_17.32.14.tif Record image shifted -0.001, 0.049 um Tracking reference shifted by 138.1,-9.9 pixels, -0.001,0.049 um Saved Z = 44 at 32.61 min Tilt = -56.00 Mean = 76 Exp = 5.347 X/Y/Z = 0.184 -0.902 4.541 Errors in X/Y Prediction -0.004 -0.029 um, 0.3 2.2 % Predicted X = 0.196 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = -0.971 StdErr = 0.021 nFit = 5 nDrop = 0 Predicted Z = 4.571 StdErr = 0.010 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 24 130, reference by 20 115 Tracking image shifted 0.016, -0.068 um; position 0.181, -0.903 Trimming A by 20 20 70 20, reference by 21 21 70 20 Drift reached 1.2 A/sec after 11.6 sec, going on Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.06 nm/sec Trimming A by 21 130, reference by 20 115 Tracking image shifted -0.000, -0.006 um; position 0.182, -0.898 Running script # 14 Trimming A by 40 24 130 20, reference by 40 24 130 20 Trimming A by 21 20 135 20, reference by 40 24 130 20 1.5 0.4 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.72892 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 42 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_045_-58.0_Oct22_17.33.27.tif Record image shifted -0.003, 0.066 um Tracking reference shifted by 187.2,-7.5 pixels, -0.003,0.066 um Saved Z = 45 at 33.84 min Tilt = -58.00 Mean = 73 Exp = 6.371 X/Y/Z = 0.185 -0.964 4.532 Errors in X/Y Prediction -0.012 0.007 um, 0.9 0.5 % Error in Z Prediction -0.039 Predicted X = 0.185 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -1.047 StdErr = 0.017 nFit = 5 nDrop = 0 Predicted Z = 4.554 StdErr = 0.017 nFit = 3 nDrop = 0 Need autofocus Trimming A by 20 20 90 20, reference by 20 20 85 20 Drift reached 0.5 A/sec after 12.3 sec, going on Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.06 nm/sec Running script # 14 1.1 0.5 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.20706 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 53 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_046_-60.0_Oct22_17.34.36.tif Record image shifted -0.001, 0.036 um Saved Z = 46 at 35.03 min Tilt = -60.00 Mean = 74 Exp = 7.891 X/Y/Z = 0.186 -1.083 4.455 Errors in X/Y Prediction 0.001 -0.036 um, 0.1 2.8 % Error in Z Prediction -0.099 Predicted X = 0.187 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -1.191 StdErr = 0.031 nFit = 5 nDrop = 0 Predicted Z = 4.499 StdErr = 0.064 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 20, reference by 21 130 Tracking image shifted 0.006, -0.111 um; position 0.181, -1.079 Trimming A by 20 20 85 20, reference by 20 20 85 20 Drift reached 0.6 A/sec after 14.8 sec, going on Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.06 nm/sec Trimming A by 20 20, reference by 21 130 Tracking image shifted -0.003, -0.014 um; position 0.185, -1.066 Running script # 14 1.1 0.5 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.06772 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 64 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_047_-62.0_Oct22_17.36.08.tif Record image shifted -0.003, 0.135 um Tracking reference shifted by 383.0,-24.6 pixels, -0.003,0.135 um Getting a new tracking reference after Record to reduce reference conflicts Saved Z = 47 at 36.58 min Tilt = -62.00 Mean = 60 Exp = 9.599 X/Y/Z = 0.188 -1.201 4.585 Errors in X/Y Prediction 0.001 -0.010 um, 0.1 0.8 % Error in Z Prediction 0.087 Predicted X = 0.173 StdErr = 0.024 nFit = 4 nDrop = 0 Predicted Y = -0.432 StdErr = 0.090 nFit = 5 nDrop = 0 Predicted Z = 1.612 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 40 40, reference by 20 20 Tracking image shifted -0.006, -0.089 um; position 0.178, -0.342 Drift reached 3.9 A/sec after 5.2 sec, going on Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.18 nm/sec Tracking image shifted -0.010, -0.014 um; position 0.190, -0.328 Running script # 14 -4.0 2.0 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.6 -1.4 nm on specimen axes Rate = 7.78214 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_048_34.0_Oct22_17.37.18.tif Record image shifted 0.002, 0.005 um Tracking reference shifted by 13.4,-6.4 pixels, 0.002,0.005 um Saved Z = 48 at 37.62 min Tilt = 34.00 Mean = 89 Exp = 1.730 X/Y/Z = 0.189 -0.331 1.577 Errors in X/Y Prediction 0.016 0.100 um, 1.3 7.7 % Error in Z Prediction -0.036 Predicted X = 0.198 StdErr = 0.026 nFit = 3 nDrop = 0 Predicted Y = -0.262 StdErr = 0.050 nFit = 5 nDrop = 0 Predicted Z = 1.554 StdErr = 0.042 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.041, 0.174 um; position 0.157, -0.435 Drift reached 3.0 A/sec after 5.4 sec, going on Running script # 14 -2.4 4.7 unbinned pixels; 1.8 0.2 nm along two shift axes; -1.6 -1.0 nm on specimen axes Rate = 9.22462 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_049_36.0_Oct22_17.37.51.tif Record image shifted 0.000, 0.008 um Tracking reference shifted by 23.0,-2.4 pixels, 0.000,0.008 um Saved Z = 49 at 38.18 min Tilt = 36.00 Mean = 89 Exp = 1.749 X/Y/Z = 0.160 -0.443 1.554 Errors in X/Y Prediction -0.038 -0.181 um, 2.9 13.8 % Predicted X = 0.171 StdErr = 0.045 nFit = 3 nDrop = 0 Predicted Y = -0.416 StdErr = 0.097 nFit = 6 nDrop = 0 Predicted Z = 1.507 StdErr = 0.048 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, 0.073 um; position 0.164, -0.490 Drift reached 4.4 A/sec after 5.4 sec, going on Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.25 nm/sec Tracking image shifted -0.009, -0.021 um; position 0.175, -0.467 Running script # 14 Trimming A by 26 20 20 20, reference by 20 20 20 20 -3.1 2.2 unbinned pixels; 1.1 0.8 nm along two shift axes; -0.7 -1.1 nm on specimen axes Rate = 6.59938 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_050_38.0_Oct22_17.38.42.tif Record image shifted -0.001, 0.002 um Tracking reference shifted by 6.3,0.9 pixels, -0.001,0.002 um Saved Z = 50 at 39.01 min Tilt = 38.00 Mean = 88 Exp = 1.776 X/Y/Z = 0.176 -0.468 1.455 Errors in X/Y Prediction 0.005 -0.051 um, 0.4 3.9 % Error in Z Prediction -0.052 Predicted X = 0.162 StdErr = 0.034 nFit = 3 nDrop = 0 Predicted Y = -0.546 StdErr = 0.096 nFit = 5 nDrop = 0 Predicted Z = 1.387 StdErr = 0.024 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Trimming A by 45 45, reference by 30 30 Tracking image shifted -0.004, -0.023 um; position 0.166, -0.523 Drift reached 2.0 A/sec after 5.5 sec, going on Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.16 nm/sec Trimming A by 35 35, reference by 30 30 Tracking image shifted -0.005, 0.000 um; position 0.172, -0.524 Running script # 14 Trimming A by 40 20 20 20, reference by 40 20 20 20 Trimming A by 40 20 20 20, reference by 40 20 20 20 -1.8 2.6 unbinned pixels; 1.1 0.3 nm along two shift axes; -0.9 -0.7 nm on specimen axes Rate = 5.56008 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_051_40.0_Oct22_17.39.27.tif Record image shifted 0.000, 0.006 um Tracking reference shifted by 18.1,-2.9 pixels, 0.000,0.006 um Saved Z = 51 at 39.79 min Tilt = 40.00 Mean = 88 Exp = 1.809 X/Y/Z = 0.173 -0.529 1.418 Errors in X/Y Prediction 0.010 0.017 um, 0.8 1.3 % Error in Z Prediction 0.031 Predicted X = 0.182 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = -0.623 StdErr = 0.095 nFit = 5 nDrop = 0 Predicted Z = 1.343 StdErr = 0.023 nFit = 5 nDrop = 0 Need regular tracking - Trimming A by 20 90, reference by 40 40 Tracking image shifted 0.012, -0.070 um; position 0.171, -0.553 Drift reached 6.7 A/sec after 5.4 sec, going on Running script # 14 Trimming A by 55 21 45 20, reference by 55 21 45 20 Trimming A by 55 21 45 20, reference by 55 21 45 20 -7.6 6.3 unbinned pixels; 3.0 1.7 nm along two shift axes; -2.0 -2.8 nm on specimen axes Rate = 17.26791 A/sec Trimming A by 55 21 45 20, reference by 55 21 45 20 -5.7 4.1 unbinned pixels; 2.0 1.4 nm along two shift axes; -1.3 -2.1 nm on specimen axes Rate = 12.3496 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_052_42.0_Oct22_17.40.07.tif Record image shifted -0.006, -0.006 um Tracking reference shifted by -15.3,19.4 pixels, -0.006,-0.006 um Saved Z = 52 at 40.44 min Tilt = 42.00 Mean = 88 Exp = 1.842 X/Y/Z = 0.182 -0.539 1.343 Errors in X/Y Prediction 0.000 0.084 um, 0.0 6.5 % Predicted X = 0.191 StdErr = 0.000 nFit = 4 nDrop = 0 Predicted Y = -1.335 StdErr = 0.023 nFit = 6 nDrop = 0 Predicted Z = 4.548 StdErr = 0.091 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Redoing image because of insufficient counts Redoing image because of insufficient counts There have been 3 failures to obtain an image with sufficient counts (1 actual vs. 1 criterion unbinned counts per sec) ONE SIDE OF THE SERIES IS BEING ENDED; GOING ON TO FINISH THE OTHER SIDE BASED ON TILT SERIES POLICY SETTINGS Errors in X/Y Prediction -0.008 0.796 um, 0.6 61.0 % Need regular tracking - Need autofocus Trimming A by 55 55, reference by 40 40 Tracking image shifted -0.002, -0.015 um; position 0.185, -0.524 Drift reached 7.4 A/sec after 5.6 sec, going on Measured defocus = -4.35 microns changed by -0.15 to target drift = 0.18 nm/sec Trimming A by 55 55, reference by 40 40 Tracking image shifted -0.014, -0.023 um; position 0.201, -0.498 Running script # 14 Trimming A by 60 20 50 20, reference by 60 20 50 20 Trimming A by 60 20 50 20, reference by 60 20 50 20 1.3 5.5 unbinned pixels; 1.7 1.1 nm along two shift axes; -2.0 0.3 nm on specimen axes Rate = 10.00473 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo8_L5G1_053_44.0_Oct22_17.41.42.tif Record image shifted 0.007, 0.022 um Tracking reference shifted by 61.2,-26.2 pixels, 0.007,0.022 um Saved Z = 53 at 42.02 min Tilt = 44.00 Mean = 89 Exp = 1.893 X/Y/Z = 0.196 -0.521 1.174 Need regular tracking - Need autofocus 2020/10/22 17:42 D:\Users\Ben\201022_Jans_cells\SEM\tomo8_L5G1.st 0 54 3838 3710 1537 -10.0 44.0 2.00 REG 42.0 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Measured defocus = -6.88 microns changed by 2.63 to target drift = 0.09 nm/sec Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.000 um; position 0.000, -0.000 Drift reached 0.3 A/sec after 5.2 sec, going on Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.02 nm/sec Tracking image shifted -0.000, 0.001 um; position 0.000, -0.001 Running script # 14 0.4 0.5 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.08867 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_000_-10.0_Oct22_17.46.52.tif Record image shifted -0.005, 0.002 um Tracking reference shifted by 6.9,12.3 pixels, -0.005,0.002 um Saved Z = 0 at -0.00 min Tilt = -10.00 Mean = 96 Exp = 1.600 X/Y/Z = 0.005 -0.003 3.543 Need regular tracking - Need autofocus Tracking image shifted -0.016, -0.072 um; position 0.021, 0.068 Drift reached 1.0 A/sec after 5.2 sec, going on Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.05 nm/sec Tracking image shifted -0.001, -0.003 um; position 0.023, 0.072 Running script # 14 -0.9 0.4 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.1 -0.3 nm on specimen axes Rate = 1.80554 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_001_-12.0_Oct22_17.47.34.tif Record image shifted 0.000, 0.002 um Tracking reference shifted by 6.8,-0.6 pixels, 0.000,0.002 um Saved Z = 1 at 0.70 min Tilt = -12.00 Mean = 94 Exp = 1.591 X/Y/Z = 0.023 0.070 3.562 Predicted X = 0.041 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.144 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.581 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.015, 0.024 um; position 0.027, 0.119 Drift reached 0.8 A/sec after 5.2 sec, going on Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.03 nm/sec Tracking image shifted 0.001, 0.003 um; position 0.026, 0.116 Running script # 14 0.3 0.1 unbinned pixels; 0.0 0.1 nm along two shift axes; -0.1 0.1 nm on specimen axes Rate = 0.63143 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_002_-14.0_Oct22_17.48.16.tif Record image shifted -0.000, 0.005 um Tracking reference shifted by 14.3,-1.1 pixels, -0.000,0.005 um Saved Z = 2 at 1.40 min Tilt = -14.00 Mean = 94 Exp = 1.607 X/Y/Z = 0.026 0.111 3.657 Errors in X/Y Prediction -0.015 -0.032 um, 1.1 2.5 % Error in Z Prediction 0.075 Need regular tracking - Need autofocus Tracking image shifted -0.024, 0.277 um; position 0.029, -0.281 Drift reached 2.8 A/sec after 5.0 sec, going on Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.12 nm/sec Tracking image shifted 0.004, 0.006 um; position 0.025, -0.288 Running script # 14 1.1 0.3 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.07504 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_003_-8.0_Oct22_17.49.02.tif Record image shifted 0.001, -0.003 um Tracking reference shifted by -7.3,-1.9 pixels, 0.001,-0.003 um Saved Z = 3 at 2.16 min Tilt = -8.00 Mean = 96 Exp = 1.591 X/Y/Z = 0.025 -0.286 3.418 Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.015 um; position 0.025, -0.301 Drift reached 1.4 A/sec after 5.4 sec, going on Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.08 nm/sec Tracking image shifted 0.000, -0.004 um; position 0.025, -0.296 Running script # 14 -1.1 0.3 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.1 -0.4 nm on specimen axes Rate = 1.97813 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_004_-6.0_Oct22_17.49.47.tif Record image shifted -0.000, -0.005 um Tracking reference shifted by -14.1,1.5 pixels, -0.000,-0.005 um Saved Z = 4 at 2.92 min Tilt = -6.00 Mean = 96 Exp = 1.580 X/Y/Z = 0.025 -0.290 3.359 Predicted X = 0.040 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = 0.174 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Z = 3.701 StdErr = 0.056 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, 0.038 um; position 0.041, 0.136 Drift reached 0.4 A/sec after 5.3 sec, going on Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.04 nm/sec Tracking image shifted 0.001, -0.003 um; position 0.041, 0.139 Running script # 14 -0.8 0.1 unbinned pixels; 0.1 0.3 nm along two shift axes; 0.0 -0.3 nm on specimen axes Rate = 1.41698 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_005_-16.0_Oct22_17.50.31.tif Record image shifted 0.000, 0.002 um Tracking reference shifted by 6.0,-1.4 pixels, 0.000,0.002 um Saved Z = 5 at 3.64 min Tilt = -16.00 Mean = 94 Exp = 1.625 X/Y/Z = 0.041 0.137 3.738 Errors in X/Y Prediction 0.001 -0.037 um, 0.1 2.8 % Error in Z Prediction 0.037 Predicted X = 0.047 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = 0.173 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Z = 3.828 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, 0.045 um; position 0.034, 0.128 Drift reached 2.1 A/sec after 5.3 sec, going on Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.09 nm/sec Tracking image shifted 0.001, 0.003 um; position 0.033, 0.123 Running script # 14 0.8 -0.0 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.0 0.3 nm on specimen axes Rate = 1.43061 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_006_-18.0_Oct22_17.51.13.tif Record image shifted 0.000, 0.007 um Tracking reference shifted by 19.0,-2.5 pixels, 0.000,0.007 um Saved Z = 6 at 4.35 min Tilt = -18.00 Mean = 94 Exp = 1.648 X/Y/Z = 0.032 0.116 3.803 Errors in X/Y Prediction -0.015 -0.056 um, 1.2 4.3 % Error in Z Prediction -0.024 Predicted X = 0.025 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.295 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.299 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.006, -0.024 um; position 0.031, -0.271 Drift reached 2.1 A/sec after 5.2 sec, going on Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.10 nm/sec Tracking image shifted -0.001, 0.005 um; position 0.032, -0.277 Running script # 14 0.8 0.1 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.40276 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_007_-4.0_Oct22_17.51.59.tif Record image shifted 0.001, -0.000 um Tracking reference shifted by -1.6,-1.7 pixels, 0.001,-0.000 um Saved Z = 7 at 5.11 min Tilt = -4.00 Mean = 96 Exp = 1.571 X/Y/Z = 0.031 -0.277 3.298 Errors in X/Y Prediction 0.006 0.018 um, 0.5 1.4 % Error in Z Prediction -0.002 Predicted X = 0.033 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.276 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Z = 3.238 StdErr = 0.001 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.009, -0.008 um; position 0.025, -0.268 Drift reached 1.3 A/sec after 5.2 sec, going on Running script # 14 -1.8 0.6 unbinned pixels; 0.4 0.5 nm along two shift axes; -0.2 -0.7 nm on specimen axes Rate = 3.42384 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_008_-2.0_Oct22_17.52.32.tif Record image shifted -0.000, -0.006 um Tracking reference shifted by -16.3,2.6 pixels, -0.000,-0.006 um Saved Z = 8 at 5.67 min Tilt = -2.00 Mean = 95 Exp = 1.559 X/Y/Z = 0.025 -0.261 3.238 Errors in X/Y Prediction -0.008 0.015 um, 0.6 1.1 % Predicted X = 0.039 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = 0.127 StdErr = 0.034 nFit = 3 nDrop = 0 Predicted Z = 3.879 StdErr = 0.012 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.005, 0.001 um; position 0.044, 0.126 Drift reached 0.2 A/sec after 5.2 sec, going on Running script # 14 -0.9 0.2 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.0 -0.3 nm on specimen axes Rate = 1.57133 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_009_-20.0_Oct22_17.53.05.tif Record image shifted -0.000, 0.004 um Tracking reference shifted by 10.1,-0.6 pixels, -0.000,0.004 um Saved Z = 9 at 6.21 min Tilt = -20.00 Mean = 93 Exp = 1.671 X/Y/Z = 0.044 0.122 3.879 Errors in X/Y Prediction 0.005 -0.004 um, 0.4 0.3 % Predicted X = 0.042 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = 0.100 StdErr = 0.048 nFit = 4 nDrop = 0 Predicted Z = 3.952 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, 0.012 um; position 0.039, 0.089 Drift reached 1.0 A/sec after 5.4 sec, going on Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.07 nm/sec Tracking image shifted -0.000, 0.003 um; position 0.039, 0.085 Running script # 14 1.0 0.3 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.77295 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_010_-22.0_Oct22_17.53.49.tif Record image shifted 0.000, 0.007 um Tracking reference shifted by 19.5,-2.9 pixels, 0.000,0.007 um Saved Z = 10 at 6.94 min Tilt = -22.00 Mean = 93 Exp = 1.702 X/Y/Z = 0.038 0.078 3.923 Errors in X/Y Prediction -0.004 -0.023 um, 0.3 1.7 % Error in Z Prediction -0.029 Predicted X = 0.027 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.247 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Z = 3.177 StdErr = 0.002 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.014, -0.014 um; position 0.042, -0.233 Drift reached 2.2 A/sec after 5.3 sec, going on Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.10 nm/sec Tracking image shifted -0.001, 0.004 um; position 0.043, -0.239 Running script # 14 0.8 0.6 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 1.78965 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_011_-0.0_Oct22_17.54.35.tif Record image shifted 0.001, 0.004 um Tracking reference shifted by 10.9,-3.1 pixels, 0.001,0.004 um Saved Z = 11 at 7.71 min Tilt = -0.00 Mean = 96 Exp = 1.554 X/Y/Z = 0.042 -0.243 3.099 Errors in X/Y Prediction 0.015 0.004 um, 1.1 0.3 % Error in Z Prediction -0.079 Predicted X = 0.044 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Y = -0.226 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Z = 3.016 StdErr = 0.035 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.016, 0.070 um; position 0.028, -0.295 Drift reached 1.0 A/sec after 5.2 sec, going on Running script # 14 -0.5 1.9 unbinned pixels; 0.7 0.0 nm along two shift axes; -0.6 -0.2 nm on specimen axes Rate = 3.4355 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_012_2.0_Oct22_17.55.08.tif Record image shifted 0.002, -0.004 um Tracking reference shifted by -10.4,-3.6 pixels, 0.002,-0.004 um Saved Z = 12 at 8.27 min Tilt = 2.00 Mean = 95 Exp = 1.543 X/Y/Z = 0.027 -0.292 3.016 Errors in X/Y Prediction -0.017 -0.066 um, 1.3 5.0 % Predicted X = 0.044 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = 0.031 StdErr = 0.030 nFit = 5 nDrop = 0 Predicted Z = 3.986 StdErr = 0.005 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, 0.014 um; position 0.050, 0.017 Drift reached 0.2 A/sec after 5.5 sec, going on Running script # 14 -0.6 0.6 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.2 -0.2 nm on specimen axes Rate = 1.49882 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_013_-24.0_Oct22_17.55.43.tif Record image shifted -0.000, 0.005 um Tracking reference shifted by 14.3,-0.3 pixels, -0.000,0.005 um Saved Z = 13 at 8.84 min Tilt = -24.00 Mean = 93 Exp = 1.732 X/Y/Z = 0.050 0.012 3.986 Errors in X/Y Prediction 0.006 -0.019 um, 0.5 1.5 % Predicted X = 0.051 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.064 StdErr = 0.033 nFit = 5 nDrop = 0 Predicted Z = 4.047 StdErr = 0.006 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.045 um; position 0.046, -0.019 Drift reached 1.8 A/sec after 5.4 sec, going on Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.08 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.047, -0.022 Running script # 14 0.8 0.4 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.52678 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_014_-26.0_Oct22_17.56.26.tif Record image shifted 0.000, 0.008 um Tracking reference shifted by 21.9,-2.0 pixels, 0.000,0.008 um Saved Z = 14 at 9.57 min Tilt = -26.00 Mean = 93 Exp = 1.762 X/Y/Z = 0.047 -0.031 4.035 Errors in X/Y Prediction -0.003 0.033 um, 0.3 2.6 % Error in Z Prediction -0.012 Predicted X = 0.021 StdErr = 0.037 nFit = 4 nDrop = 0 Predicted Y = -0.355 StdErr = 0.046 nFit = 4 nDrop = 0 Predicted Z = 2.927 StdErr = 0.042 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.010, -0.131 um; position 0.032, -0.224 Drift reached 2.0 A/sec after 5.2 sec, going on Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.07 nm/sec Tracking image shifted -0.002, 0.001 um; position 0.035, -0.225 Running script # 14 0.2 0.8 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.3 0.0 nm on specimen axes Rate = 1.4355 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_015_4.0_Oct22_17.57.14.tif Record image shifted -0.002, 0.003 um Tracking reference shifted by 9.3,4.9 pixels, -0.002,0.003 um Saved Z = 15 at 10.37 min Tilt = 4.00 Mean = 95 Exp = 1.542 X/Y/Z = 0.037 -0.229 2.931 Errors in X/Y Prediction 0.016 0.127 um, 1.2 9.7 % Error in Z Prediction 0.004 Predicted X = 0.029 StdErr = 0.038 nFit = 4 nDrop = 0 Predicted Y = -0.227 StdErr = 0.076 nFit = 5 nDrop = 0 Predicted Z = 2.834 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.026, 0.019 um; position 0.055, -0.246 Drift reached 1.2 A/sec after 5.2 sec, going on Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.05 nm/sec Tracking image shifted -0.002, -0.000 um; position 0.057, -0.246 Running script # 14 0.3 0.8 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.44983 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_016_6.0_Oct22_17.57.59.tif Record image shifted -0.000, 0.001 um Tracking reference shifted by 3.5,-0.2 pixels, -0.000,0.001 um Saved Z = 16 at 11.11 min Tilt = 6.00 Mean = 95 Exp = 1.535 X/Y/Z = 0.057 -0.247 2.889 Errors in X/Y Prediction 0.028 -0.020 um, 2.2 1.6 % Error in Z Prediction 0.055 Predicted X = 0.055 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = -0.113 StdErr = 0.029 nFit = 6 nDrop = 0 Predicted Z = 4.097 StdErr = 0.007 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.005, -0.039 um; position 0.060, -0.074 Drift reached 1.0 A/sec after 5.4 sec, going on Running script # 14 0.1 0.9 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 0.0 nm on specimen axes Rate = 1.55613 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_017_-28.0_Oct22_17.58.33.tif Record image shifted -0.000, 0.007 um Tracking reference shifted by 19.6,-0.4 pixels, -0.000,0.007 um Saved Z = 17 at 11.69 min Tilt = -28.00 Mean = 93 Exp = 1.805 X/Y/Z = 0.061 -0.081 4.097 Errors in X/Y Prediction 0.006 0.032 um, 0.5 2.5 % Predicted X = 0.063 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.129 StdErr = 0.016 nFit = 5 nDrop = 0 Predicted Z = 4.157 StdErr = 0.008 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, -0.023 um; position 0.056, -0.106 Drift reached 1.8 A/sec after 5.7 sec, going on Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.057, -0.109 Running script # 14 0.7 0.6 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.65664 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_018_-30.0_Oct22_17.59.18.tif Record image shifted 0.001, 0.009 um Tracking reference shifted by 24.9,-4.0 pixels, 0.001,0.009 um Saved Z = 18 at 12.43 min Tilt = -30.00 Mean = 93 Exp = 1.845 X/Y/Z = 0.057 -0.118 4.160 Errors in X/Y Prediction -0.007 0.010 um, 0.5 0.8 % Error in Z Prediction 0.003 Predicted X = 0.099 StdErr = 0.010 nFit = 4 nDrop = 0 Predicted Y = -0.238 StdErr = 0.055 nFit = 6 nDrop = 0 Predicted Z = 2.805 StdErr = 0.036 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.061, -0.021 um; position 0.038, -0.217 Drift reached 1.5 A/sec after 5.2 sec, going on Running script # 14 0.4 1.8 unbinned pixels; 0.5 0.3 nm along two shift axes; -0.6 0.1 nm on specimen axes Rate = 3.16871 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_019_8.0_Oct22_17.59.56.tif Record image shifted 0.001, 0.001 um Tracking reference shifted by 3.6,-3.5 pixels, 0.001,0.001 um Saved Z = 19 at 13.07 min Tilt = 8.00 Mean = 95 Exp = 1.537 X/Y/Z = 0.038 -0.218 2.805 Errors in X/Y Prediction -0.061 0.019 um, 4.6 1.5 % Predicted X = 0.046 StdErr = 0.029 nFit = 3 nDrop = 0 Predicted Y = -0.193 StdErr = 0.053 nFit = 6 nDrop = 0 Predicted Z = 2.733 StdErr = 0.043 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, 0.106 um; position 0.037, -0.299 Drift reached 1.7 A/sec after 5.3 sec, going on Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.06 nm/sec Tracking image shifted 0.001, 0.003 um; position 0.037, -0.303 Running script # 14 0.5 1.0 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.1 nm on specimen axes Rate = 2.00267 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_020_10.0_Oct22_18.00.41.tif Record image shifted -0.001, 0.004 um Tracking reference shifted by 12.3,0.5 pixels, -0.001,0.004 um Saved Z = 20 at 13.82 min Tilt = 10.00 Mean = 95 Exp = 1.532 X/Y/Z = 0.038 -0.307 2.702 Errors in X/Y Prediction -0.008 -0.114 um, 0.6 8.7 % Error in Z Prediction -0.031 Predicted X = 0.064 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.149 StdErr = 0.012 nFit = 5 nDrop = 0 Predicted Z = 4.217 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.032 um; position 0.065, -0.181 Drift reached 1.3 A/sec after 5.6 sec, going on Running script # 14 0.7 1.1 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.2 nm on specimen axes Rate = 2.19523 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_021_-32.0_Oct22_18.01.17.tif Record image shifted 0.000, 0.009 um Tracking reference shifted by 25.8,-2.9 pixels, 0.000,0.009 um Saved Z = 21 at 14.42 min Tilt = -32.00 Mean = 92 Exp = 1.893 X/Y/Z = 0.066 -0.191 4.217 Errors in X/Y Prediction 0.001 -0.042 um, 0.1 3.2 % Predicted X = 0.065 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Y = -0.237 StdErr = 0.022 nFit = 6 nDrop = 0 Predicted Z = 4.276 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.007 um; position 0.064, -0.244 Drift reached 1.1 A/sec after 5.7 sec, going on Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.08 nm/sec Tracking image shifted -0.001, 0.003 um; position 0.065, -0.247 Running script # 14 1.0 0.6 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 2.08651 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_022_-34.0_Oct22_18.02.01.tif Record image shifted 0.000, 0.010 um Tracking reference shifted by 27.7,-2.6 pixels, 0.000,0.010 um Saved Z = 22 at 15.15 min Tilt = -34.00 Mean = 92 Exp = 1.952 X/Y/Z = 0.065 -0.258 4.235 Errors in X/Y Prediction 0.000 -0.021 um, 0.0 1.6 % Error in Z Prediction -0.041 Predicted X = 0.025 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = -0.392 StdErr = 0.062 nFit = 5 nDrop = 1 Predicted Z = 2.633 StdErr = 0.029 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.023, -0.126 um; position 0.048, -0.266 Drift reached 5.4 A/sec after 5.2 sec, going on Running script # 14 -8.5 4.0 unbinned pixels; 2.3 2.3 nm along two shift axes; -1.1 -3.1 nm on specimen axes Rate = 16.46517 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_023_12.0_Oct22_18.02.39.tif Record image shifted -0.002, -0.007 um Tracking reference shifted by -20.5,8.0 pixels, -0.002,-0.007 um Saved Z = 23 at 15.78 min Tilt = 12.00 Mean = 94 Exp = 1.532 X/Y/Z = 0.053 -0.253 2.633 Errors in X/Y Prediction 0.028 0.140 um, 2.1 10.7 % Predicted X = 0.058 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.269 StdErr = 0.099 nFit = 5 nDrop = 0 Predicted Z = 2.555 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.056 um; position 0.052, -0.325 Drift reached 2.9 A/sec after 5.3 sec, going on Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.14 nm/sec Tracking image shifted -0.001, 0.005 um; position 0.054, -0.331 Running script # 14 2.2 1.5 unbinned pixels; 0.2 0.9 nm along two shift axes; -0.6 0.7 nm on specimen axes Rate = 4.76241 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_024_14.0_Oct22_18.03.23.tif Record image shifted 0.002, 0.016 um Tracking reference shifted by 44.2,-9.6 pixels, 0.002,0.016 um Saved Z = 24 at 16.52 min Tilt = 14.00 Mean = 95 Exp = 1.541 X/Y/Z = 0.052 -0.348 2.488 Errors in X/Y Prediction -0.005 -0.079 um, 0.4 6.0 % Error in Z Prediction -0.067 Predicted X = 0.071 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.333 StdErr = 0.014 nFit = 6 nDrop = 0 Predicted Z = 4.300 StdErr = 0.024 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.000, 0.017 um; position 0.071, -0.350 Drift reached 0.7 A/sec after 5.8 sec, going on Running script # 14 0.2 1.4 unbinned pixels; 0.4 0.2 nm along two shift axes; -0.5 0.0 nm on specimen axes Rate = 2.48581 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_025_-36.0_Oct22_18.04.01.tif Record image shifted -0.001, 0.011 um Tracking reference shifted by 32.2,-0.6 pixels, -0.001,0.011 um Saved Z = 25 at 17.15 min Tilt = -36.00 Mean = 92 Exp = 2.013 X/Y/Z = 0.073 -0.361 4.300 Errors in X/Y Prediction 0.002 -0.029 um, 0.1 2.2 % Predicted X = 0.075 StdErr = 0.011 nFit = 4 nDrop = 0 Predicted Y = -0.463 StdErr = 0.017 nFit = 6 nDrop = 0 Predicted Z = 4.353 StdErr = 0.026 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, -0.093 um; position 0.073, -0.370 Drift reached 2.0 A/sec after 5.9 sec, going on Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.08 nm/sec Tracking image shifted -0.001, 0.004 um; position 0.075, -0.375 Running script # 14 0.8 0.7 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 1.89938 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_026_-38.0_Oct22_18.04.46.tif Record image shifted 0.001, 0.011 um Tracking reference shifted by 31.8,-6.0 pixels, 0.001,0.011 um Saved Z = 26 at 17.91 min Tilt = -38.00 Mean = 91 Exp = 2.087 X/Y/Z = 0.074 -0.387 4.311 Errors in X/Y Prediction -0.000 0.076 um, 0.0 5.8 % Error in Z Prediction -0.043 Predicted X = 0.062 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.398 StdErr = 0.112 nFit = 5 nDrop = 0 Predicted Z = 2.412 StdErr = 0.044 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, -0.071 um; position 0.055, -0.328 Drift reached 10.1 A/sec after 5.2 sec, going on Running script # 14 -17.4 6.8 unbinned pixels; 4.3 4.9 nm along two shift axes; -1.8 -6.3 nm on specimen axes Rate = 32.64871 A/sec -10.8 2.1 unbinned pixels; 1.9 3.3 nm along two shift axes; -0.4 -3.8 nm on specimen axes Rate = 19.2194 A/sec -12.0 5.9 unbinned pixels; 3.4 3.3 nm along two shift axes; -1.7 -4.4 nm on specimen axes Rate = 23.50458 A/sec -6.0 3.4 unbinned pixels; 1.8 1.6 nm along two shift axes; -1.0 -2.2 nm on specimen axes Rate = 12.09227 A/sec Drift is low enough after shot 4 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_027_16.0_Oct22_18.05.41.tif Record image shifted -0.003, -0.016 um Tracking reference shifted by -44.5,12.6 pixels, -0.003,-0.016 um Saved Z = 27 at 18.82 min Tilt = 16.00 Mean = 94 Exp = 1.535 X/Y/Z = 0.065 -0.290 2.412 Errors in X/Y Prediction 0.003 0.109 um, 0.2 8.3 % Predicted X = 0.069 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.266 StdErr = 0.119 nFit = 5 nDrop = 0 Predicted Z = 2.322 StdErr = 0.049 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, 0.086 um; position 0.060, -0.352 Drift reached 4.1 A/sec after 5.2 sec, going on Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.20 nm/sec Tracking image shifted -0.001, 0.005 um; position 0.062, -0.359 Running script # 14 2.2 1.9 unbinned pixels; 0.4 1.0 nm along two shift axes; -0.7 0.7 nm on specimen axes Rate = 5.1564 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_028_18.0_Oct22_18.06.26.tif Record image shifted 0.003, 0.027 um Tracking reference shifted by 74.6,-16.0 pixels, 0.003,0.027 um Saved Z = 28 at 19.57 min Tilt = 18.00 Mean = 95 Exp = 1.554 X/Y/Z = 0.060 -0.386 2.308 Errors in X/Y Prediction -0.009 -0.120 um, 0.7 9.2 % Error in Z Prediction -0.014 Predicted X = 0.081 StdErr = 0.009 nFit = 4 nDrop = 0 Predicted Y = -0.446 StdErr = 0.050 nFit = 5 nDrop = 0 Predicted Z = 4.366 StdErr = 0.027 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, 0.030 um; position 0.080, -0.476 Drift reached 1.6 A/sec after 6.1 sec, going on Running script # 14 1.4 1.5 unbinned pixels; 0.3 0.7 nm along two shift axes; -0.6 0.4 nm on specimen axes Rate = 3.69482 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_029_-40.0_Oct22_18.07.04.tif Record image shifted 0.000, 0.013 um Tracking reference shifted by 35.6,-3.3 pixels, 0.000,0.013 um Saved Z = 29 at 20.20 min Tilt = -40.00 Mean = 91 Exp = 2.173 X/Y/Z = 0.081 -0.490 4.366 Errors in X/Y Prediction 0.000 -0.044 um, 0.0 3.3 % Predicted X = 0.085 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.554 StdErr = 0.041 nFit = 6 nDrop = 0 Predicted Z = 4.411 StdErr = 0.030 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.058 um; position 0.080, -0.496 Drift reached 1.3 A/sec after 6.0 sec, going on Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.07 nm/sec Tracking image shifted -0.002, 0.003 um; position 0.082, -0.500 Running script # 14 0.7 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.86152 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_030_-42.0_Oct22_18.07.50.tif Record image shifted 0.001, 0.012 um Tracking reference shifted by 33.1,-5.5 pixels, 0.001,0.012 um Saved Z = 30 at 20.97 min Tilt = -42.00 Mean = 90 Exp = 2.268 X/Y/Z = 0.081 -0.512 4.378 Errors in X/Y Prediction -0.003 0.042 um, 0.2 3.2 % Error in Z Prediction -0.033 Predicted X = 0.086 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.550 StdErr = 0.063 nFit = 5 nDrop = 0 Predicted Z = 4.417 StdErr = 0.004 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, 0.015 um; position 0.078, -0.566 Drift reached 0.2 A/sec after 6.3 sec, going on Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.02 nm/sec Tracking image shifted -0.001, 0.001 um; position 0.078, -0.566 Running script # 14 0.1 0.2 unbinned pixels; 0.0 0.1 nm along two shift axes; -0.1 0.0 nm on specimen axes Rate = 0.36698 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_031_-44.0_Oct22_18.08.37.tif Record image shifted 0.000, 0.014 um Tracking reference shifted by 39.1,-4.3 pixels, 0.000,0.014 um Saved Z = 31 at 21.76 min Tilt = -44.00 Mean = 90 Exp = 2.386 X/Y/Z = 0.078 -0.580 4.418 Errors in X/Y Prediction -0.008 -0.030 um, 0.6 2.3 % Error in Z Prediction 0.001 Predicted X = 0.066 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.449 StdErr = 0.122 nFit = 5 nDrop = 0 Predicted Z = 2.204 StdErr = 0.022 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.010, -0.069 um; position 0.057, -0.381 Drift reached 9.8 A/sec after 5.2 sec, going on Running script # 14 -14.2 6.7 unbinned pixels; 3.9 3.9 nm along two shift axes; -1.9 -5.2 nm on specimen axes Rate = 27.51511 A/sec -8.1 2.2 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.5 -2.9 nm on specimen axes Rate = 14.70776 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_032_20.0_Oct22_18.09.21.tif Record image shifted -0.005, -0.016 um Tracking reference shifted by -45.1,17.6 pixels, -0.005,-0.016 um Saved Z = 32 at 22.48 min Tilt = 20.00 Mean = 93 Exp = 1.547 X/Y/Z = 0.066 -0.351 2.204 Errors in X/Y Prediction -0.001 0.098 um, 0.0 7.5 % Predicted X = 0.065 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.343 StdErr = 0.120 nFit = 5 nDrop = 0 Predicted Z = 2.105 StdErr = 0.026 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.015, 0.074 um; position 0.049, -0.417 Drift reached 4.1 A/sec after 5.2 sec, going on Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.17 nm/sec Tracking image shifted -0.002, 0.006 um; position 0.053, -0.425 Running script # 14 2.2 2.5 unbinned pixels; 0.6 1.0 nm along two shift axes; -1.0 0.7 nm on specimen axes Rate = 6.00293 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_033_22.0_Oct22_18.10.06.tif Record image shifted 0.006, 0.028 um Tracking reference shifted by 77.5,-23.7 pixels, 0.006,0.028 um Saved Z = 33 at 23.22 min Tilt = 22.00 Mean = 95 Exp = 1.576 X/Y/Z = 0.048 -0.453 2.169 Errors in X/Y Prediction -0.017 -0.110 um, 1.3 8.4 % Error in Z Prediction 0.063 Predicted X = 0.046 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = -0.542 StdErr = 0.112 nFit = 5 nDrop = 0 Predicted Z = 2.082 StdErr = 0.026 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, -0.070 um; position 0.046, -0.472 Drift reached 6.6 A/sec after 5.2 sec, going on Running script # 14 -9.4 6.0 unbinned pixels; 3.1 2.4 nm along two shift axes; -1.8 -3.5 nm on specimen axes Rate = 19.56286 A/sec -7.8 4.7 unbinned pixels; 2.5 2.0 nm along two shift axes; -1.4 -2.9 nm on specimen axes Rate = 16.00455 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_034_24.0_Oct22_18.10.44.tif Record image shifted -0.004, -0.004 um Tracking reference shifted by -10.7,11.8 pixels, -0.004,-0.004 um Saved Z = 34 at 23.94 min Tilt = 24.00 Mean = 93 Exp = 1.568 X/Y/Z = 0.055 -0.458 2.082 Errors in X/Y Prediction 0.009 0.083 um, 0.7 6.4 % Predicted X = 0.077 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.637 StdErr = 0.060 nFit = 5 nDrop = 0 Predicted Z = 4.453 StdErr = 0.004 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.012, -0.035 um; position 0.089, -0.601 Drift reached 2.6 A/sec after 6.5 sec, going on Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.13 nm/sec Tracking image shifted -0.002, 0.006 um; position 0.092, -0.609 Running script # 14 1.6 0.6 unbinned pixels; 0.0 0.6 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 3.00975 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_035_-46.0_Oct22_18.11.42.tif Record image shifted 0.000, 0.016 um Tracking reference shifted by 44.7,-4.4 pixels, 0.000,0.016 um Saved Z = 35 at 24.84 min Tilt = -46.00 Mean = 89 Exp = 2.519 X/Y/Z = 0.092 -0.625 4.469 Errors in X/Y Prediction 0.015 0.012 um, 1.2 0.9 % Error in Z Prediction 0.017 Predicted X = 0.095 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.653 StdErr = 0.050 nFit = 5 nDrop = 0 Predicted Z = 4.501 StdErr = 0.012 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, 0.044 um; position 0.088, -0.697 Drift reached 1.1 A/sec after 6.6 sec, going on Running script # 14 0.5 0.3 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 1.04327 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_036_-48.0_Oct22_18.12.18.tif Record image shifted 0.002, 0.018 um Tracking reference shifted by 50.6,-9.8 pixels, 0.002,0.018 um Saved Z = 36 at 25.44 min Tilt = -48.00 Mean = 89 Exp = 2.681 X/Y/Z = 0.087 -0.716 4.501 Errors in X/Y Prediction -0.008 -0.063 um, 0.6 4.8 % Predicted X = 0.094 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = -0.812 StdErr = 0.028 nFit = 5 nDrop = 0 Predicted Z = 4.540 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.054 um; position 0.090, -0.758 Drift reached 1.2 A/sec after 6.9 sec, going on Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.08 nm/sec Tracking image shifted -0.000, 0.004 um; position 0.091, -0.763 Running script # 14 0.7 0.0 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.0 0.2 nm on specimen axes Rate = 1.14579 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_037_-50.0_Oct22_18.13.08.tif Record image shifted -0.001, 0.017 um Tracking reference shifted by 48.3,-1.8 pixels, -0.001,0.017 um Saved Z = 37 at 26.29 min Tilt = -50.00 Mean = 88 Exp = 2.874 X/Y/Z = 0.092 -0.780 4.477 Errors in X/Y Prediction -0.003 0.032 um, 0.2 2.4 % Error in Z Prediction -0.063 Predicted X = 0.085 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Y = -0.864 StdErr = 0.029 nFit = 5 nDrop = 0 Predicted Z = 4.516 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.013, -0.052 um; position 0.097, -0.812 Drift reached 1.4 A/sec after 7.0 sec, going on Running script # 14 0.8 0.5 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.70606 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_038_-52.0_Oct22_18.13.45.tif Record image shifted -0.001, 0.021 um Tracking reference shifted by 59.8,-3.7 pixels, -0.001,0.021 um Saved Z = 38 at 26.91 min Tilt = -52.00 Mean = 87 Exp = 3.110 X/Y/Z = 0.098 -0.834 4.516 Errors in X/Y Prediction 0.014 0.030 um, 1.1 2.3 % Predicted X = 0.045 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = -0.486 StdErr = 0.102 nFit = 5 nDrop = 0 Predicted Z = 2.002 StdErr = 0.030 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.012, -0.080 um; position 0.056, -0.407 Drift reached 7.2 A/sec after 5.2 sec, going on Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.24 nm/sec Tracking image shifted -0.009, -0.015 um; position 0.068, -0.388 Running script # 14 -1.1 4.7 unbinned pixels; 1.7 0.2 nm along two shift axes; -1.6 -0.5 nm on specimen axes Rate = 8.42801 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_039_26.0_Oct22_18.14.37.tif Record image shifted 0.006, 0.020 um Tracking reference shifted by 54.4,-20.5 pixels, 0.006,0.020 um Saved Z = 39 at 27.74 min Tilt = 26.00 Mean = 95 Exp = 1.595 X/Y/Z = 0.064 -0.407 1.993 Errors in X/Y Prediction 0.019 0.079 um, 1.5 6.1 % Error in Z Prediction -0.009 Predicted X = 0.072 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.391 StdErr = 0.116 nFit = 5 nDrop = 0 Predicted Z = 1.920 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.026, 0.142 um; position 0.046, -0.533 Drift reached 4.7 A/sec after 5.4 sec, going on Running script # 14 2.9 5.7 unbinned pixels; 1.6 1.6 nm along two shift axes; -2.1 0.8 nm on specimen axes Rate = 11.32753 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_040_28.0_Oct22_18.15.10.tif Record image shifted -0.001, 0.017 um Tracking reference shifted by 47.8,-2.5 pixels, -0.001,0.017 um Saved Z = 40 at 28.30 min Tilt = 28.00 Mean = 94 Exp = 1.603 X/Y/Z = 0.050 -0.553 1.920 Errors in X/Y Prediction -0.021 -0.161 um, 1.6 12.4 % Predicted X = 0.033 StdErr = 0.017 nFit = 4 nDrop = 0 Predicted Y = -0.567 StdErr = 0.161 nFit = 5 nDrop = 0 Predicted Z = 1.842 StdErr = 0.027 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.019, 0.041 um; position 0.051, -0.608 Drift reached 5.6 A/sec after 5.4 sec, going on Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.35 nm/sec Tracking image shifted -0.007, -0.014 um; position 0.060, -0.591 Running script # 14 -4.1 2.5 unbinned pixels; 1.3 1.1 nm along two shift axes; -0.7 -1.5 nm on specimen axes Rate = 8.43648 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_041_30.0_Oct22_18.15.55.tif Record image shifted 0.000, 0.001 um Tracking reference shifted by 3.4,-0.9 pixels, 0.000,0.001 um Saved Z = 41 at 29.05 min Tilt = 30.00 Mean = 93 Exp = 1.623 X/Y/Z = 0.060 -0.590 1.926 Errors in X/Y Prediction 0.028 -0.024 um, 2.1 1.8 % Error in Z Prediction 0.084 Predicted X = 0.055 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Y = -0.734 StdErr = 0.115 nFit = 5 nDrop = 0 Predicted Z = 1.847 StdErr = 0.069 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.142 um; position 0.049, -0.591 Drift reached 4.4 A/sec after 5.2 sec, going on Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.22 nm/sec Tracking image shifted -0.007, -0.007 um; position 0.058, -0.583 Running script # 14 -3.5 4.6 unbinned pixels; 2.0 0.6 nm along two shift axes; -1.5 -1.4 nm on specimen axes Rate = 10.23154 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_042_32.0_Oct22_18.16.40.tif Record image shifted 0.001, 0.013 um Tracking reference shifted by 36.8,-6.3 pixels, 0.001,0.013 um Saved Z = 42 at 29.79 min Tilt = 32.00 Mean = 94 Exp = 1.652 X/Y/Z = 0.059 -0.595 1.840 Errors in X/Y Prediction 0.004 0.139 um, 0.3 10.6 % Error in Z Prediction -0.007 Predicted X = 0.104 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.901 StdErr = 0.035 nFit = 5 nDrop = 0 Predicted Z = 4.541 StdErr = 0.044 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, -0.048 um; position 0.096, -0.853 Drift reached 2.3 A/sec after 7.4 sec, going on Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.11 nm/sec Tracking image shifted -0.003, 0.008 um; position 0.099, -0.863 Running script # 14 1.4 0.8 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 2.77224 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_043_-54.0_Oct22_18.17.38.tif Trimming A by 20 25, reference by 20 20 Record image shifted 0.001, 0.025 um Tracking reference shifted by 71.0,-9.6 pixels, 0.001,0.025 um Saved Z = 43 at 30.79 min Tilt = -54.00 Mean = 85 Exp = 3.394 X/Y/Z = 0.098 -0.888 4.606 Errors in X/Y Prediction -0.006 0.013 um, 0.4 1.0 % Error in Z Prediction 0.065 Predicted X = 0.103 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.920 StdErr = 0.015 nFit = 5 nDrop = 0 Predicted Z = 4.619 StdErr = 0.052 nFit = 4 nDrop = 0 Need autofocus Drift reached 0.3 A/sec after 7.5 sec, going on Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.06 nm/sec Running script # 14 Trimming A by 21 30 35 20, reference by 21 30 35 20 Trimming A by 21 30 35 20, reference by 21 30 35 20 0.7 0.4 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.42206 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_044_-56.0_Oct22_18.18.29.tif Trimming A by 55 60, reference by 30 30 Record image shifted 0.006, 0.057 um Saved Z = 44 at 31.64 min Tilt = -56.00 Mean = 86 Exp = 3.783 X/Y/Z = 0.097 -0.978 4.704 Errors in X/Y Prediction -0.005 -0.058 um, 0.4 4.4 % Error in Z Prediction 0.085 Predicted X = 0.097 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -1.054 StdErr = 0.024 nFit = 5 nDrop = 0 Predicted Z = 4.717 StdErr = 0.072 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.090 um; position 0.094, -0.964 Drift reached 1.7 A/sec after 8.4 sec, going on Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.10 nm/sec Trimming A by 30 30, reference by 20 20 Tracking image shifted -0.002, 0.004 um; position 0.097, -0.970 Running script # 14 Trimming A by 20 40 35 20, reference by 20 40 35 20 Trimming A by 20 40 35 20, reference by 20 40 35 20 1.3 0.6 unbinned pixels; 0.0 0.5 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.45695 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_045_-58.0_Oct22_18.19.28.tif Trimming A by 75 75, reference by 65 65 Record image shifted 0.001, 0.047 um Tracking reference shifted by 133.2,-13.9 pixels, 0.001,0.047 um Saved Z = 45 at 32.65 min Tilt = -58.00 Mean = 82 Exp = 4.196 X/Y/Z = 0.096 -1.018 4.727 Errors in X/Y Prediction -0.001 0.037 um, 0.0 2.8 % Error in Z Prediction 0.010 Predicted X = 0.095 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -1.088 StdErr = 0.025 nFit = 6 nDrop = 0 Predicted Z = 4.809 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Errors in X/Y Prediction 0.001 0.071 um, 0.1 5.4 % Error in Z Prediction -0.082 Need regular tracking - Need autofocus Tracking image shifted -0.003, -0.014 um; position 0.062, -0.581 Drift reached 10.0 A/sec after 5.2 sec, going on Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.27 nm/sec Tracking image shifted -0.012, -0.027 um; position 0.077, -0.548 Running script # 14 -1.2 5.0 unbinned pixels; 1.8 0.2 nm along two shift axes; -1.7 -0.6 nm on specimen axes Rate = 8.98849 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_046_34.0_Oct22_18.20.41.tif Record image shifted 0.001, 0.010 um Tracking reference shifted by 28.4,-5.5 pixels, 0.001,0.010 um Saved Z = 46 at 33.82 min Tilt = 34.00 Mean = 94 Exp = 1.667 X/Y/Z = 0.078 -0.558 1.753 Need regular tracking - Need autofocus Tracking image shifted 0.031, 0.097 um; position 0.047, -0.655 Drift reached 4.4 A/sec after 5.4 sec, going on Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.17 nm/sec Tracking image shifted -0.004, 0.003 um; position 0.053, -0.659 Running script # 14 -1.2 2.3 unbinned pixels; 0.9 0.1 nm along two shift axes; -0.8 -0.5 nm on specimen axes Rate = 4.63565 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_047_36.0_Oct22_18.21.27.tif Record image shifted -0.001, 0.014 um Tracking reference shifted by 40.1,-1.3 pixels, -0.001,0.014 um Saved Z = 47 at 34.57 min Tilt = 36.00 Mean = 94 Exp = 1.692 X/Y/Z = 0.054 -0.673 1.766 Predicted X = 0.031 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.788 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 1.780 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.026, -0.100 um; position 0.057, -0.688 Drift reached 6.9 A/sec after 5.5 sec, going on Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.28 nm/sec Tracking image shifted -0.008, -0.016 um; position 0.068, -0.669 Running script # 14 -3.1 1.7 unbinned pixels; 0.9 0.8 nm along two shift axes; -0.5 -1.1 nm on specimen axes Rate = 6.17587 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_048_38.0_Oct22_18.22.12.tif Record image shifted -0.000, 0.012 um Tracking reference shifted by 32.9,-2.6 pixels, -0.000,0.012 um Saved Z = 48 at 35.34 min Tilt = 38.00 Mean = 93 Exp = 1.719 X/Y/Z = 0.069 -0.679 1.692 Errors in X/Y Prediction 0.038 0.109 um, 2.9 8.3 % Error in Z Prediction -0.088 Predicted X = 0.058 StdErr = 0.028 nFit = 3 nDrop = 0 Predicted Y = -0.758 StdErr = 0.081 nFit = 3 nDrop = 0 Predicted Z = 1.677 StdErr = 0.065 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.006, 0.003 um; position 0.064, -0.761 Drift reached 3.2 A/sec after 5.5 sec, going on Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.19 nm/sec Tracking image shifted -0.005, -0.004 um; position 0.070, -0.756 Running script # 14 -1.0 2.0 unbinned pixels; 0.8 0.1 nm along two shift axes; -0.7 -0.4 nm on specimen axes Rate = 3.87176 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_049_40.0_Oct22_18.22.58.tif Record image shifted 0.001, 0.016 um Tracking reference shifted by 44.2,-5.8 pixels, 0.001,0.016 um Saved Z = 49 at 36.09 min Tilt = 40.00 Mean = 93 Exp = 1.752 X/Y/Z = 0.070 -0.771 1.675 Errors in X/Y Prediction 0.012 -0.013 um, 0.9 1.0 % Error in Z Prediction -0.002 Predicted X = 0.080 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.806 StdErr = 0.064 nFit = 3 nDrop = 0 Predicted Z = 1.620 StdErr = 0.042 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.012, -0.018 um; position 0.068, -0.787 Drift reached 5.4 A/sec after 5.5 sec, going on Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.27 nm/sec Tracking image shifted -0.007, -0.015 um; position 0.077, -0.770 Running script # 14 -3.2 1.5 unbinned pixels; 0.9 0.9 nm along two shift axes; -0.4 -1.2 nm on specimen axes Rate = 6.19179 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_050_42.0_Oct22_18.23.43.tif Record image shifted 0.000, 0.014 um Tracking reference shifted by 40.8,-4.9 pixels, 0.000,0.014 um Saved Z = 50 at 36.85 min Tilt = 42.00 Mean = 93 Exp = 1.785 X/Y/Z = 0.077 -0.784 1.574 Errors in X/Y Prediction -0.003 0.022 um, 0.2 1.7 % Error in Z Prediction -0.046 Predicted X = 0.080 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.849 StdErr = 0.059 nFit = 3 nDrop = 0 Predicted Z = 1.528 StdErr = 0.062 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.015, -0.028 um; position 0.064, -0.821 Drift reached 2.5 A/sec after 5.4 sec, going on Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.19 nm/sec Tracking image shifted -0.004, -0.005 um; position 0.070, -0.815 Running script # 14 -1.1 1.7 unbinned pixels; 0.7 0.2 nm along two shift axes; -0.6 -0.4 nm on specimen axes Rate = 3.52375 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_051_44.0_Oct22_18.24.29.tif Record image shifted 0.000, 0.017 um Tracking reference shifted by 49.0,-4.3 pixels, 0.000,0.017 um Saved Z = 51 at 37.62 min Tilt = 44.00 Mean = 93 Exp = 1.826 X/Y/Z = 0.070 -0.832 1.524 Errors in X/Y Prediction -0.010 0.017 um, 0.7 1.3 % Error in Z Prediction -0.005 Predicted X = 0.073 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.831 StdErr = 0.076 nFit = 4 nDrop = 0 Predicted Z = 1.440 StdErr = 0.038 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.022 um; position 0.072, -0.853 Drift reached 5.9 A/sec after 5.6 sec, going on Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.29 nm/sec Tracking image shifted -0.007, -0.013 um; position 0.081, -0.837 Running script # 14 -2.8 1.3 unbinned pixels; 0.8 0.8 nm along two shift axes; -0.4 -1.0 nm on specimen axes Rate = 5.39651 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_052_46.0_Oct22_18.25.16.tif Record image shifted 0.001, 0.016 um Tracking reference shifted by 46.0,-5.6 pixels, 0.001,0.016 um Saved Z = 52 at 38.39 min Tilt = 46.00 Mean = 92 Exp = 1.865 X/Y/Z = 0.081 -0.852 1.428 Errors in X/Y Prediction 0.008 -0.021 um, 0.6 1.6 % Error in Z Prediction -0.011 Predicted X = 0.080 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.851 StdErr = 0.045 nFit = 5 nDrop = 0 Predicted Z = 1.353 StdErr = 0.024 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.012, 0.033 um; position 0.068, -0.884 Drift reached 2.5 A/sec after 5.6 sec, going on Running script # 14 -2.8 3.2 unbinned pixels; 1.4 0.5 nm along two shift axes; -1.0 -1.1 nm on specimen axes Rate = 7.37699 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\tomo9_L5G1_053_48.0_Oct22_18.25.50.tif Record image shifted -0.001, 0.019 um Tracking reference shifted by 53.3,-2.5 pixels, -0.001,0.019 um Saved Z = 53 at 38.96 min Tilt = 48.00 Mean = 92 Exp = 1.924 X/Y/Z = 0.071 -0.901 1.353 2020/10/22 18:27 D:\Users\Ben\201022_Jans_cells\SEM\tomo9_L5G1.st 0 54 3838 3710 1537 -10.0 48.0 2.00 REG 39.0 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Measured defocus = -6.22 microns changed by 1.97 to target drift = 0.12 nm/sec Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.17 nm/sec Trimming A by 50 50, reference by 50 50 Angle = -4.40, increment = 0.60, shift = -28.5 -4.5 pixels, -0.066 um Y Trimming A by 55 55, reference by 50 50 Angle = -3.20, increment = 1.80, shift = -65.2 -10.0 pixels, -0.150 um Y Trimming A by 65 70, reference by 50 50 Angle = 0.41, increment = 5.41, shift = -212.8 -33.2 pixels, -0.491 um Y Trimming A by 75 75, reference by 50 50 Angle = 3.00, increment = 8.00, shift = -307.9 -48.0 pixels, -0.711 um Y Rough eucentricity: changing Z by 5.10 to -235.07, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, -0.000 Trimming A by 75 75, reference by 70 75 Angle = 18.00, shift = 6.8, 13.9 pixels, 0.030, 0.019 um, IS = -0.030, -0.019 Trimming A by 75 75, reference by 70 70 Angle = 11.00, shift = -22.0, 5.9 pixels, 0.019, -0.048 um, IS = -0.049, 0.029 Trimming A by 75 75, reference by 75 75 Angle = 4.00, shift = 19.1, 12.5 pixels, 0.023, 0.047 um, IS = -0.072, -0.017 Trimming A by 85 21 20 60, reference by 75 23 20 55 Angle = -4.00, shift = -18.4, -18.5 pixels, -0.037, -0.046 um, IS = -0.035, 0.029 Trimming A by 85 85, reference by 80 80 Angle = -11.00, shift = -22.6, -0.6 pixels, 0.004, -0.051 um, IS = -0.040, 0.081 Trimming A by 80 85, reference by 85 85 Angle = -18.00, shift = 45.5, 14.0 pixels, 0.020, 0.107 um, IS = -0.060, -0.026 Trimming A by 85 90, reference by 85 85 Angle = -24.00, shift = 65.8, 21.4 pixels, 0.032, 0.155 um, IS = -0.092, -0.181 General solution delZ -0.10, delY 1.39, yZero 0.04 solution with assumed YZero 0.01: delZ -0.10, delY 0.86 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.10 microns and lateral displacement of -1.39 microns Measured defocus = -5.55 microns changed by 1.30 to target drift = 0.03 nm/sec Measured defocus = -3.48 microns changed by -0.77 to target drift = 0.10 nm/sec Stored imaging state in a new parameter set for item # 1, label 1 Measured defocus = -6.23 microns changed by 1.98 to target drift = 0.06 nm/sec Stored imaging state in a new parameter set for item # 4, label 4 Stored imaging state in a new parameter set for item # 6, label 6 Angle = -4.40, increment = 0.60, shift = 5.5 0.3 pixels, 0.013 um Y Angle = -3.20, increment = 1.80, shift = 37.6 5.5 pixels, 0.087 um Y Angle = 0.41, increment = 5.41, shift = 94.5 10.8 pixels, 0.217 um Y Angle = 3.00, increment = 8.00, shift = 141.9 15.8 pixels, 0.326 um Y Rough eucentricity: changing Z by -2.34 to -237.30, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = 30.0, 15.1 pixels, 0.027, 0.072 um, IS = -0.027, -0.072 Angle = 11.00, shift = 13.8, 6.2 pixels, 0.011, 0.033 um, IS = -0.037, -0.105 Angle = 4.00, shift = 35.2, 12.1 pixels, 0.019, 0.083 um, IS = -0.056, -0.188 Angle = -4.00, shift = 4.0, -19.4 pixels, -0.045, 0.004 um, IS = -0.011, -0.192 Angle = -11.00, shift = 0.3, -5.8 pixels, -0.013, -0.001 um, IS = 0.002, -0.191 Angle = -18.00, shift = 67.1, 13.1 pixels, 0.013, 0.156 um, IS = -0.010, -0.347 Angle = -24.00, shift = 84.7, 17.5 pixels, 0.018, 0.197 um, IS = -0.028, -0.543 General solution delZ -0.53, delY 1.36, yZero -0.16 solution with assumed YZero -0.19: delZ -0.53, delY 0.90 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.53 microns and lateral displacement of -1.36 microns Measured defocus = -2.12 microns changed by -2.13 to target drift = 0.21 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.20 nm/sec Stored imaging state in a new parameter set for item # 8, label 8 Stored imaging state in a new parameter set for item # 10, label 10 Stored imaging state in a new parameter set for item # 12, label 12 Stored imaging state in a new parameter set for item # 14, label 14 Measured defocus = -0.09 microns changed by -4.16 to target drift = 0.01 nm/sec Measured defocus = -9.02 microns changed by 4.77 to target drift = 0.05 nm/sec Stored imaging state in a new parameter set for item # 16, label 16 Backlash adjustment to stage coordinates is 0.13, -0.10 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 18, label 18 Measured defocus = -8.17 microns changed by 3.92 to target drift = 0.07 nm/sec Trimming A by 21 20 20 25, reference by 20 20 20 20 Backlash adjustment to stage coordinates is 0.37, 0.05 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 20, label 20 Eucentric focus value of 0.00000 saved for mag 42000x nanoprobe Focus area changed from stored state, axis position -3.20 angle 0, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_01.st Running script 1 at item 1 (124.66, 116.64) 19:30:21 Refine ZLP: additional offset = 6.2 eV; total offset = 6.2 eV Item 1: Stage: 124.66 116.64 -235 Label: 1 Note: Sec 0 - anchor_maps.map Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Target defocus is: -4.25 um Target defocus is: -4.50 um Target defocus = -4.5 Trimming A by 23 135, reference by 23 135 Angle = -4.40, increment = 0.60, shift = 1.2 -2.9 pixels, 0.002 um Y Trimming A by 33 130, reference by 23 135 Angle = -3.20, increment = 1.80, shift = -7.5 -6.8 pixels, -0.019 um Y Trimming A by 24 130, reference by 23 135 Angle = 0.41, increment = 5.41, shift = -22.4 -12.7 pixels, -0.054 um Y Trimming A by 24 130, reference by 23 135 Angle = 3.00, increment = 8.00, shift = -34.1 -17.4 pixels, -0.082 um Y Rough eucentricity: changing Z by 0.59 to -234.37, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 21 140, reference by 21 145 Angle = 18.00, shift = 12.8, 11.1 pixels, 0.022, 0.032 um, IS = -0.022, -0.032 Trimming A by 24 135, reference by 22 140 Angle = 11.00, shift = 5.6, 7.1 pixels, 0.015, 0.015 um, IS = -0.037, -0.047 Trimming A by 21 135, reference by 20 135 Angle = 4.00, shift = 29.5, 10.8 pixels, 0.017, 0.070 um, IS = -0.053, -0.116 Trimming A by 20 50 20 120, reference by 24 55 20 125 Angle = -4.00, shift = -6.2, -20.4 pixels, -0.045, -0.019 um, IS = -0.009, -0.097 Trimming A by 24 130, reference by 30 125 Angle = -11.00, shift = -19.2, -4.0 pixels, -0.004, -0.045 um, IS = -0.004, -0.053 Trimming A by 20 135, reference by 24 130 Angle = -18.00, shift = 57.8, 13.2 pixels, 0.015, 0.134 um, IS = -0.020, -0.187 Trimming A by 39 135, reference by 20 135 Angle = -24.00, shift = 60.0, 16.9 pixels, 0.023, 0.140 um, IS = -0.043, -0.327 General solution delZ -0.30, delY 1.02, yZero -0.07 solution with assumed YZero -0.11: delZ -0.30, delY 0.50 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.30 microns and lateral displacement of -1.02 microns Trimming A by 20 34 20 130, reference by 21 46 20 135 Disparity in stage position after aligning to center of map frame: 0.08 -0.41 Aligning to target with image shift equivalent to stage shift: 0.08 -0.42 Disparity in stage position after aligning to map item itself: 0.09 -0.38 (align shift 0.01 0.01) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 124.67 116.67 -234.07 Trimming A by 40 23 75 65, reference by 40 23 75 65 -0.1 1.6 unbinned pixels; 0.5 0.2 nm along two shift axes; -0.6 -0.1 nm on specimen axes Rate = 2.78121 A/sec Drift is low enough after shot 1 Measured defocus = -2.96 microns changed by -1.54 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -1.536 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_000_-10.0_Oct22_19.38.10.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 81.0 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.088 0.393 IS units -1152.6 -134.5 unbinned pixels; need stage 0.082 -0.409 if reset Tilting to -8 Correcting tilt backlash Trimming A by 32 95, reference by 30 70 Trimming A by 30 70, reference by 30 70 9.3 0.5 unbinned pixels; 0.9 3.1 nm along two shift axes; -0.5 3.2 nm on specimen axes Rate = 16.27477 A/sec Trimming A by 21 75, reference by 30 70 4.0 1.1 unbinned pixels; 0.1 1.4 nm along two shift axes; -0.5 1.4 nm on specimen axes Rate = 7.25199 A/sec Drift is low enough after shot 2 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.08 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -1.655 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_001_-8.0_Oct22_19.39.14.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 89.3 In SetExposureForMean 90, change by a factor of 1.0079 would require too small a change in exposure time Image shift -0.140 0.388 IS units -1189.5 11.6 unbinned pixels; need stage 0.033 -0.425 if reset Tilting to -12 -12.00 degrees Trimming A by 42 90, reference by 30 70 Trimming A by 20 70, reference by 30 70 -5.3 4.8 unbinned pixels; 2.2 1.2 nm along two shift axes; -1.5 -2.0 nm on specimen axes Rate = 12.53268 A/sec Trimming A by 20 70, reference by 30 70 2.1 2.9 unbinned pixels; 0.7 1.0 nm along two shift axes; -1.1 0.6 nm on specimen axes Rate = 6.31497 A/sec Drift is low enough after shot 2 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.03 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -1.473 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_002_-12.0_Oct22_19.40.18.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 89.1 In SetExposureForMean 90, change by a factor of 1.0097 would require too small a change in exposure time Image shift -0.188 0.481 IS units -1488.0 54.7 unbinned pixels; need stage 0.027 -0.540 if reset Tilting to -14 Trimming A by 21 65, reference by 20 70 Trimming A by 20 70, reference by 20 70 -2.3 0.1 unbinned pixels; 0.3 0.7 nm along two shift axes; 0.0 -0.8 nm on specimen axes Rate = 4.02813 A/sec Drift is low enough after shot 1 Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.04 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -1.334 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_003_-14.0_Oct22_19.41.16.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 88.6 In SetExposureForMean 90, change by a factor of 1.0162 would require too small a change in exposure time Image shift -0.219 0.537 IS units -1671.1 84.8 unbinned pixels; need stage 0.022 -0.612 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees Trimming A by 20 55, reference by 20 70 Trimming A by 20 75, reference by 20 70 8.3 -2.8 unbinned pixels; 1.9 2.4 nm along two shift axes; 0.7 3.0 nm on specimen axes Rate = 15.36567 A/sec Trimming A by 20 75, reference by 20 70 8.2 -0.1 unbinned pixels; 1.0 2.7 nm along two shift axes; -0.2 2.9 nm on specimen axes Rate = 14.32263 A/sec Trimming A by 20 75, reference by 20 70 4.1 -0.2 unbinned pixels; 0.5 1.3 nm along two shift axes; -0.1 1.4 nm on specimen axes Rate = 7.19552 A/sec Drift is low enough after shot 3 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.08 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -1.738 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_004_-6.0_Oct22_19.42.27.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 89.4 In SetExposureForMean 90, change by a factor of 1.0068 would require too small a change in exposure time Image shift -0.117 0.316 IS units -972.5 16.9 unbinned pixels; need stage 0.024 -0.346 if reset Tilting to -4 Correcting tilt backlash Trimming A by 20 55, reference by 20 75 Trimming A by 20 75, reference by 20 75 -6.1 -3.2 unbinned pixels; 0.3 2.4 nm along two shift axes; 1.3 -2.0 nm on specimen axes Rate = 12.13604 A/sec Trimming A by 20 75, reference by 20 75 -0.1 2.1 unbinned pixels; 0.7 0.2 nm along two shift axes; -0.7 -0.1 nm on specimen axes Rate = 3.62558 A/sec Drift is low enough after shot 2 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.836 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_005_-4.0_Oct22_19.43.32.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 89.2 In SetExposureForMean 90, change by a factor of 1.0088 would require too small a change in exposure time Image shift -0.087 0.261 IS units -793.1 -10.2 unbinned pixels; need stage 0.028 -0.281 if reset Tilting to -16 -16.00 degrees Trimming A by 20 60, reference by 20 70 Trimming A by 20 70, reference by 20 70 -3.3 -5.4 unbinned pixels; 1.4 1.7 nm along two shift axes; 2.0 -1.0 nm on specimen axes Rate = 11.22546 A/sec Trimming A by 20 70, reference by 20 70 -2.1 2.4 unbinned pixels; 1.1 0.4 nm along two shift axes; -0.8 -0.8 nm on specimen axes Rate = 5.65118 A/sec Drift is low enough after shot 2 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -1.238 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_006_-16.0_Oct22_19.44.37.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 88.4 In SetExposureForMean 90, change by a factor of 1.0187 would require too small a change in exposure time Image shift -0.189 0.443 IS units -1386.7 92.0 unbinned pixels; need stage 0.011 -0.513 if reset Tilting to -18 Trimming A by 20 75, reference by 20 70 Trimming A by 20 70, reference by 20 70 2.1 -1.2 unbinned pixels; 0.6 0.5 nm along two shift axes; 0.4 0.8 nm on specimen axes Rate = 4.22689 A/sec Drift is low enough after shot 1 Measured defocus = -4.60 microns changed by 0.10 to target drift = 0.08 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -1.139 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_007_-18.0_Oct22_19.45.34.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 87.6 In SetExposureForMean 90, change by a factor of 1.0278 would require too small a change in exposure time Image shift -0.174 0.410 IS units -1282.8 82.2 unbinned pixels; need stage 0.011 -0.480 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees Trimming A by 40 70, reference by 20 75 Trimming A by 20 80, reference by 20 75 8.0 0.4 unbinned pixels; 0.8 2.7 nm along two shift axes; -0.4 2.8 nm on specimen axes Rate = 14.03943 A/sec Trimming A by 20 75, reference by 20 75 6.4 2.2 unbinned pixels; 0.0 2.4 nm along two shift axes; -1.0 2.2 nm on specimen axes Rate = 11.95012 A/sec Trimming A by 20 80, reference by 20 75 2.1 1.0 unbinned pixels; 0.1 0.8 nm along two shift axes; -0.4 0.7 nm on specimen axes Rate = 4.03543 A/sec Drift is low enough after shot 3 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -1.889 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_008_-2.0_Oct22_19.46.52.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 89.1 In SetExposureForMean 90, change by a factor of 1.0096 would require too small a change in exposure time Image shift -0.122 0.297 IS units -925.9 49.4 unbinned pixels; need stage 0.011 -0.329 if reset Tilting to 0 Correcting tilt backlash Trimming A by 20 70, reference by 20 80 Trimming A by 20 80, reference by 20 80 -6.0 -3.4 unbinned pixels; 0.4 2.4 nm along two shift axes; 1.4 -2.0 nm on specimen axes Rate = 12.10741 A/sec Trimming A by 20 80, reference by 20 80 -0.5 2.0 unbinned pixels; 0.7 0.1 nm along two shift axes; -0.7 -0.2 nm on specimen axes Rate = 3.64206 A/sec Drift is low enough after shot 2 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -1.958 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_009_-0.0_Oct22_19.47.57.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 88.8 In SetExposureForMean 90, change by a factor of 1.0131 would require too small a change in exposure time Image shift -0.093 0.244 IS units -753.1 20.6 unbinned pixels; need stage 0.016 -0.267 if reset Tilting to -20 -20.00 degrees Trimming A by 20 65, reference by 20 70 Trimming A by 20 70, reference by 20 70 -1.6 -6.2 unbinned pixels; 1.9 1.3 nm along two shift axes; 2.2 -0.4 nm on specimen axes Rate = 11.30316 A/sec Trimming A by 20 70, reference by 20 70 -0.6 2.7 unbinned pixels; 1.0 0.1 nm along two shift axes; -0.9 -0.3 nm on specimen axes Rate = 4.88051 A/sec Drift is low enough after shot 2 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.02 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -1.055 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_010_-20.0_Oct22_19.49.03.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 86.7 In SetExposureForMean 90, change by a factor of 1.0381 would require too small a change in exposure time Image shift -0.136 0.301 IS units -948.3 84.3 unbinned pixels; need stage 0.000 -0.360 if reset Tilting to -22 Trimming A by 20 70, reference by 20 65 Trimming A by 21 70, reference by 20 65 3.0 -0.2 unbinned pixels; 0.4 1.0 nm along two shift axes; -0.0 1.1 nm on specimen axes Rate = 5.2786 A/sec Drift is low enough after shot 1 Measured defocus = -4.60 microns changed by 0.10 to target drift = 0.04 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.949 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_011_-22.0_Oct22_19.50.00.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 86.1 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.127 0.278 IS units -878.2 81.0 unbinned pixels; need stage -0.001 -0.338 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees Trimming A by 45 75, reference by 20 80 Trimming A by 25 75, reference by 20 80 6.7 -0.8 unbinned pixels; 1.0 2.1 nm along two shift axes; 0.1 2.4 nm on specimen axes Rate = 11.82061 A/sec Trimming A by 25 75, reference by 20 80 6.0 2.9 unbinned pixels; 0.3 2.3 nm along two shift axes; -1.2 2.0 nm on specimen axes Rate = 11.78823 A/sec Trimming A by 25 75, reference by 20 80 2.3 1.3 unbinned pixels; 0.2 0.9 nm along two shift axes; -0.5 0.8 nm on specimen axes Rate = 4.65931 A/sec Drift is low enough after shot 3 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = -2.050 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_012_2.0_Oct22_19.51.12.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 96.3 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.124 0.263 IS units -834.8 87.6 unbinned pixels; need stage -0.004 -0.298 if reset Tilting to 4 Correcting tilt backlash Trimming A by 20 75, reference by 25 75 Trimming A by 40 75, reference by 25 75 -6.9 -0.6 unbinned pixels; 0.6 2.4 nm along two shift axes; 0.4 -2.4 nm on specimen axes Rate = 12.18479 A/sec Trimming A by 35 75, reference by 25 75 -2.9 1.4 unbinned pixels; 0.8 0.8 nm along two shift axes; -0.4 -1.1 nm on specimen axes Rate = 5.62704 A/sec Drift is low enough after shot 2 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.11 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -2.132 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_013_4.0_Oct22_19.52.18.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 87.9 In SetExposureForMean 90, change by a factor of 1.0235 would require too small a change in exposure time Image shift -0.111 0.236 IS units -748.3 78.6 unbinned pixels; need stage -0.004 -0.268 if reset Tilting to -24 -24.00 degrees Trimming A by 20 70, reference by 20 65 Trimming A by 20 70, reference by 20 65 -0.5 -7.2 unbinned pixels; 2.3 1.0 nm along two shift axes; 2.6 0.0 nm on specimen axes Rate = 12.82549 A/sec Trimming A by 20 70, reference by 20 65 -0.3 2.0 unbinned pixels; 0.7 0.1 nm along two shift axes; -0.7 -0.2 nm on specimen axes Rate = 3.62 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.02 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.00 nm/sec Defocus = -0.882 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_014_-24.0_Oct22_19.53.23.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 85.1 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.077 0.136 IS units -443.1 80.5 unbinned pixels; need stage -0.014 -0.174 if reset Tilting to -26 Trimming A by 20 65, reference by 20 70 Trimming A by 20 65, reference by 20 70 3.0 -0.7 unbinned pixels; 0.6 0.9 nm along two shift axes; 0.2 1.1 nm on specimen axes Rate = 5.39657 A/sec Drift is low enough after shot 1 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.04 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.787 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_015_-26.0_Oct22_19.54.21.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 91.7 In SetExposureForMean 90, change by a factor of 0.9822 would require too small a change in exposure time Image shift -0.069 0.116 IS units -381.8 78.7 unbinned pixels; need stage -0.015 -0.153 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees Trimming A by 60 70, reference by 35 75 Trimming A by 40 75, reference by 35 75 4.6 0.5 unbinned pixels; 0.4 1.6 nm along two shift axes; -0.3 1.6 nm on specimen axes Rate = 8.1104 A/sec Trimming A by 40 75, reference by 35 75 3.5 2.9 unbinned pixels; 0.5 1.5 nm along two shift axes; -1.1 1.1 nm on specimen axes Rate = 7.94357 A/sec Drift is low enough after shot 2 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -2.238 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_016_6.0_Oct22_19.55.29.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 94.9 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.141 0.272 IS units -875.4 126.0 unbinned pixels; need stage -0.016 -0.315 if reset Tilting to 8 Correcting tilt backlash Trimming A by 45 55, reference by 40 75 Trimming A by 50 75, reference by 40 75 -8.9 -0.2 unbinned pixels; 1.0 3.0 nm along two shift axes; 0.3 -3.1 nm on specimen axes Rate = 15.57421 A/sec Trimming A by 50 75, reference by 40 75 -5.5 2.0 unbinned pixels; 1.3 1.6 nm along two shift axes; -0.5 -2.0 nm on specimen axes Rate = 10.224 A/sec Trimming A by 50 75, reference by 40 75 -5.7 2.0 unbinned pixels; 1.3 1.6 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 10.58347 A/sec Trimming A by 50 75, reference by 40 75 -4.8 1.4 unbinned pixels; 1.0 1.4 nm along two shift axes; -0.3 -1.7 nm on specimen axes Rate = 8.68556 A/sec Trimming A by 50 75, reference by 40 75 -4.4 1.5 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.07157 A/sec Trimming A by 50 75, reference by 40 75 -3.5 0.8 unbinned pixels; 0.7 1.1 nm along two shift axes; -0.2 -1.2 nm on specimen axes Rate = 6.2653 A/sec Drift is low enough after shot 6 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.09 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -2.313 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_017_8.0_Oct22_19.56.57.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 86.3 In SetExposureForMean 90, change by a factor of 1.0425 would require too small a change in exposure time Image shift -0.130 0.254 IS units -816.4 111.8 unbinned pixels; need stage -0.013 -0.295 if reset Tilting to -28 -28.00 degrees Trimming A by 20 70, reference by 20 65 Trimming A by 20 65, reference by 20 65 -0.8 -6.8 unbinned pixels; 2.2 1.1 nm along two shift axes; 2.4 -0.1 nm on specimen axes Rate = 12.03882 A/sec Trimming A by 20 65, reference by 20 65 -0.3 2.1 unbinned pixels; 0.7 0.2 nm along two shift axes; -0.7 -0.2 nm on specimen axes Rate = 3.67918 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.02 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.752 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_018_-28.0_Oct22_19.58.04.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 82.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.023 -0.009 IS units 2.1 71.9 unbinned pixels; need stage -0.025 -0.002 if reset Tilting to -30 Trimming A by 25 65, reference by 20 65 Trimming A by 20 65, reference by 20 65 -0.2 -2.7 unbinned pixels; 0.9 0.4 nm along two shift axes; 1.0 -0.0 nm on specimen axes Rate = 4.82371 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -0.672 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_019_-30.0_Oct22_19.59.03.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 88.7 In SetExposureForMean 90, change by a factor of 1.0156 would require too small a change in exposure time Image shift -0.002 -0.057 IS units 152.0 61.1 unbinned pixels; need stage -0.026 0.060 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees Trimming A by 85 85, reference by 50 75 Trimming A by 60 65, reference by 50 75 1.5 2.5 unbinned pixels; 0.6 0.8 nm along two shift axes; -0.9 0.5 nm on specimen axes Rate = 5.11569 A/sec Drift is low enough after shot 1 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.08 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -2.408 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_020_10.0_Oct22_20.00.06.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 93.1 In SetExposureForMean 90, change by a factor of 0.9670 would require too small a change in exposure time Image shift -0.175 0.306 IS units -1000.8 184.4 unbinned pixels; need stage -0.033 -0.365 if reset Tilting to 12 Correcting tilt backlash Trimming A by 55 55, reference by 60 65 Trimming A by 70 70, reference by 60 65 -9.9 1.0 unbinned pixels; 1.5 3.2 nm along two shift axes; -0.1 -3.5 nm on specimen axes Rate = 17.52571 A/sec Trimming A by 65 65, reference by 60 65 -7.3 3.0 unbinned pixels; 1.8 2.1 nm along two shift axes; -0.8 -2.6 nm on specimen axes Rate = 13.74106 A/sec Trimming A by 65 65, reference by 60 65 -6.6 1.9 unbinned pixels; 1.4 1.9 nm along two shift axes; -0.5 -2.4 nm on specimen axes Rate = 12.00438 A/sec Trimming A by 65 65, reference by 60 65 -4.9 1.3 unbinned pixels; 1.0 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 8.89802 A/sec Trimming A by 65 65, reference by 60 65 -3.9 0.9 unbinned pixels; 0.7 1.2 nm along two shift axes; -0.2 -1.4 nm on specimen axes Rate = 7.00905 A/sec Drift is low enough after shot 5 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -2.499 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_021_12.0_Oct22_20.01.29.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 91.9 In SetExposureForMean 90, change by a factor of 0.9794 would require too small a change in exposure time Image shift -0.166 0.302 IS units -980.4 164.1 unbinned pixels; need stage -0.026 -0.360 if reset Tilting to -32 -32.00 degrees Trimming A by 21 75, reference by 20 65 Trimming A by 20 65, reference by 20 65 -0.0 -5.5 unbinned pixels; 1.8 0.7 nm along two shift axes; 1.9 0.2 nm on specimen axes Rate = 9.73679 A/sec Trimming A by 20 65, reference by 20 65 0.1 2.5 unbinned pixels; 0.8 0.3 nm along two shift axes; -0.9 -0.0 nm on specimen axes Rate = 4.39662 A/sec Drift is low enough after shot 2 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.02 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.613 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_022_-32.0_Oct22_20.02.36.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 87.0 In SetExposureForMean 90, change by a factor of 1.0364 would require too small a change in exposure time Image shift 0.037 -0.174 IS units 507.4 65.2 unbinned pixels; need stage -0.038 0.209 if reset Tilting to -34 Trimming A by 21 70, reference by 20 65 Trimming A by 20 65, reference by 20 65 1.2 -2.3 unbinned pixels; 0.9 0.1 nm along two shift axes; 0.8 0.5 nm on specimen axes Rate = 4.57602 A/sec Drift is low enough after shot 1 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.576 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_023_-34.0_Oct22_20.03.34.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 85.3 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift 0.055 -0.217 IS units 641.7 55.5 unbinned pixels; need stage -0.039 0.272 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees Trimming A by 20 20, reference by 65 65 Trimming A by 75 75, reference by 65 65 -12.9 5.9 unbinned pixels; 3.5 3.6 nm along two shift axes; -1.7 -4.7 nm on specimen axes Rate = 24.86579 A/sec Trimming A by 75 75, reference by 65 65 -4.0 2.9 unbinned pixels; 1.4 1.0 nm along two shift axes; -0.9 -1.5 nm on specimen axes Rate = 8.76864 A/sec Trimming A by 75 75, reference by 65 65 -4.8 2.0 unbinned pixels; 1.2 1.3 nm along two shift axes; -0.6 -1.7 nm on specimen axes Rate = 9.06687 A/sec Trimming A by 75 75, reference by 65 65 -2.0 1.1 unbinned pixels; 0.6 0.5 nm along two shift axes; -0.3 -0.7 nm on specimen axes Rate = 4.02342 A/sec Drift is low enough after shot 4 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.09 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -2.628 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_024_14.0_Oct22_20.04.54.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 97.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.195 0.333 IS units -1094.5 213.4 unbinned pixels; need stage -0.040 -0.406 if reset Tilting to 16 Correcting tilt backlash Trimming A by 45 45, reference by 75 75 Trimming A by 22 120, reference by 75 75 36.2 -6.1 unbinned pixels; 6.2 11.2 nm along two shift axes; 1.0 12.9 nm on specimen axes Rate = 64.298 A/sec Trimming A by 20 20, reference by 75 75 -35.4 6.2 unbinned pixels; 6.2 11.0 nm along two shift axes; -1.1 -12.6 nm on specimen axes Rate = 62.99628 A/sec Trimming A by 20 120, reference by 75 75 1.6 2.6 unbinned pixels; 0.7 0.8 nm along two shift axes; -0.9 0.5 nm on specimen axes Rate = 5.35775 A/sec Drift is low enough after shot 3 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.06 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.713 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_025_16.0_Oct22_20.06.05.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 89.1 In SetExposureForMean 90, change by a factor of 1.0104 would require too small a change in exposure time Image shift -0.161 0.270 IS units -888.6 180.6 unbinned pixels; need stage -0.035 -0.333 if reset Tilting to -36 -36.00 degrees Trimming A by 20 65, reference by 20 65 Trimming A by 20 65, reference by 20 65 0.6 -4.6 unbinned pixels; 1.6 0.3 nm along two shift axes; 1.6 0.4 nm on specimen axes Rate = 8.18913 A/sec Trimming A by 20 65, reference by 20 65 0.2 2.7 unbinned pixels; 0.9 0.4 nm along two shift axes; -1.0 -0.0 nm on specimen axes Rate = 4.84295 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.01 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.535 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_026_-36.0_Oct22_20.07.13.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 83.3 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift 0.084 -0.313 IS units 930.1 68.1 unbinned pixels; need stage -0.052 0.404 if reset Tilting to -38 Trimming A by 20 65, reference by 20 65 Trimming A by 20 60, reference by 20 65 3.5 -0.5 unbinned pixels; 0.6 1.1 nm along two shift axes; 0.1 1.2 nm on specimen axes Rate = 6.20793 A/sec Drift is low enough after shot 1 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.07 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -0.476 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_027_-38.0_Oct22_20.08.11.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 88.1 In SetExposureForMean 90, change by a factor of 1.0238 would require too small a change in exposure time Image shift 0.093 -0.332 IS units 990.3 62.3 unbinned pixels; need stage -0.052 0.442 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -14.6 5.1 unbinned pixels; 3.4 4.2 nm along two shift axes; -1.3 -5.3 nm on specimen axes Rate = 27.15796 A/sec -6.7 2.9 unbinned pixels; 1.7 1.9 nm along two shift axes; -0.8 -2.4 nm on specimen axes Rate = 12.77232 A/sec -5.6 0.2 unbinned pixels; 0.7 1.8 nm along two shift axes; 0.1 -2.0 nm on specimen axes Rate = 9.87109 A/sec -5.6 3.1 unbinned pixels; 1.7 1.5 nm along two shift axes; -0.9 -2.1 nm on specimen axes Rate = 11.24505 A/sec -4.9 1.6 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.4 -1.8 nm on specimen axes Rate = 8.9619 A/sec -2.6 0.8 unbinned pixels; 0.6 0.8 nm along two shift axes; -0.2 -0.9 nm on specimen axes Rate = 4.73637 A/sec Drift is low enough after shot 6 Measured defocus = -4.37 microns changed by -0.13 to target drift = 0.11 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.849 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_028_18.0_Oct22_20.09.43.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 94.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.195 0.321 IS units -1062.0 225.0 unbinned pixels; need stage -0.045 -0.403 if reset Tilting to 20 Correcting tilt backlash Trimming A by 65 65, reference by 20 20 -5.1 -0.2 unbinned pixels; 0.5 1.7 nm along two shift axes; 0.2 -1.8 nm on specimen axes Rate = 8.97702 A/sec -2.8 4.9 unbinned pixels; 2.0 0.3 nm along two shift axes; -1.6 -1.1 nm on specimen axes Rate = 9.99277 A/sec -0.1 3.1 unbinned pixels; 1.1 0.3 nm along two shift axes; -1.1 -0.1 nm on specimen axes Rate = 5.52807 A/sec Drift is low enough after shot 3 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.06 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.08 nm/sec Defocus = -2.929 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_029_20.0_Oct22_20.10.54.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 85.7 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.152 0.223 IS units -753.2 202.8 unbinned pixels; need stage -0.047 -0.290 if reset Tilting to -40 -40.00 degrees Trimming A by 20 65, reference by 20 60 Trimming A by 20 60, reference by 20 60 -0.1 -3.6 unbinned pixels; 1.2 0.5 nm along two shift axes; 1.3 0.1 nm on specimen axes Rate = 6.44404 A/sec Drift is low enough after shot 1 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.03 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.438 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_030_-40.0_Oct22_20.11.57.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 85.8 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift 0.123 -0.433 IS units 1292.7 77.6 unbinned pixels; need stage -0.066 0.593 if reset Tilting to -42 Trimming A by 25 65, reference by 20 60 Trimming A by 20 65, reference by 20 60 3.1 -4.4 unbinned pixels; 1.8 0.5 nm along two shift axes; 1.4 1.2 nm on specimen axes Rate = 9.35727 A/sec Trimming A by 20 65, reference by 20 60 3.7 1.6 unbinned pixels; 0.1 1.4 nm along two shift axes; -0.7 1.2 nm on specimen axes Rate = 7.08587 A/sec Drift is low enough after shot 2 Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.422 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_031_-42.0_Oct22_20.13.00.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 89.5 In SetExposureForMean 90, change by a factor of 1.0078 would require too small a change in exposure time Image shift 0.145 -0.473 IS units 1425.2 55.4 unbinned pixels; need stage -0.063 0.676 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -14.2 4.0 unbinned pixels; 3.0 4.2 nm along two shift axes; -1.0 -5.1 nm on specimen axes Rate = 25.92367 A/sec -8.8 4.1 unbinned pixels; 2.4 2.4 nm along two shift axes; -1.2 -3.2 nm on specimen axes Rate = 16.92214 A/sec -5.6 2.5 unbinned pixels; 1.5 1.5 nm along two shift axes; -0.7 -2.0 nm on specimen axes Rate = 10.72912 A/sec -5.9 1.8 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.4 -2.1 nm on specimen axes Rate = 10.7458 A/sec -5.4 0.3 unbinned pixels; 0.7 1.8 nm along two shift axes; 0.1 -1.9 nm on specimen axes Rate = 9.55629 A/sec -1.9 2.1 unbinned pixels; 0.9 0.4 nm along two shift axes; -0.7 -0.7 nm on specimen axes Rate = 4.94343 A/sec Drift is low enough after shot 6 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.11 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -3.070 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_032_22.0_Oct22_20.14.33.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 98.1 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.196 0.285 IS units -964.2 262.2 unbinned pixels; need stage -0.061 -0.377 if reset Tilting to 24 Correcting tilt backlash -7.8 -2.1 unbinned pixels; 0.2 2.8 nm along two shift axes; 1.0 -2.7 nm on specimen axes Rate = 14.1856 A/sec -4.0 5.6 unbinned pixels; 2.3 0.7 nm along two shift axes; -1.9 -1.6 nm on specimen axes Rate = 12.14453 A/sec -0.4 2.1 unbinned pixels; 0.7 0.1 nm along two shift axes; -0.7 -0.2 nm on specimen axes Rate = 3.7576 A/sec Drift is low enough after shot 3 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.08 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -3.146 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_033_24.0_Oct22_20.15.44.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 88.8 In SetExposureForMean 90, change by a factor of 1.0150 would require too small a change in exposure time Image shift -0.141 0.153 IS units -552.6 239.5 unbinned pixels; need stage -0.065 -0.222 if reset Tilting to -44 -44.00 degrees Trimming A by 20 70, reference by 20 65 Trimming A by 21 65, reference by 20 65 2.9 -4.7 unbinned pixels; 1.9 0.4 nm along two shift axes; 1.6 1.2 nm on specimen axes Rate = 9.67528 A/sec Trimming A by 20 60, reference by 20 65 -0.0 2.3 unbinned pixels; 0.8 0.3 nm along two shift axes; -0.8 -0.1 nm on specimen axes Rate = 4.11793 A/sec Drift is low enough after shot 2 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.03 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.359 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_034_-44.0_Oct22_20.16.53.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 80.3 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift 0.177 -0.573 IS units 1726.6 64.4 unbinned pixels; need stage -0.075 0.846 if reset Tilting to -46 Trimming A by 21 70, reference by 21 65 Trimming A by 20 60, reference by 21 65 1.8 -5.1 unbinned pixels; 1.9 0.0 nm along two shift axes; 1.7 0.8 nm on specimen axes Rate = 9.46978 A/sec Trimming A by 21 65, reference by 21 65 3.3 1.8 unbinned pixels; 0.2 1.3 nm along two shift axes; -0.7 1.1 nm on specimen axes Rate = 6.58659 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.321 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_035_-46.0_Oct22_20.17.56.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 89.4 In SetExposureForMean 90, change by a factor of 1.0097 would require too small a change in exposure time Image shift 0.201 -0.619 IS units 1874.8 41.3 unbinned pixels; need stage -0.072 0.952 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -23.5 6.0 unbinned pixels; 4.7 7.1 nm along two shift axes; -1.4 -8.4 nm on specimen axes Rate = 42.56212 A/sec -5.9 0.8 unbinned pixels; 1.0 1.9 nm along two shift axes; -0.1 -2.1 nm on specimen axes Rate = 10.52934 A/sec -5.6 2.3 unbinned pixels; 1.4 1.6 nm along two shift axes; -0.6 -2.0 nm on specimen axes Rate = 10.60054 A/sec -5.6 1.5 unbinned pixels; 1.1 1.7 nm along two shift axes; -0.3 -2.0 nm on specimen axes Rate = 10.1425 A/sec -3.8 1.0 unbinned pixels; 0.8 1.1 nm along two shift axes; -0.2 -1.4 nm on specimen axes Rate = 6.90376 A/sec Drift is low enough after shot 5 Measured defocus = -4.39 microns changed by -0.11 to target drift = 0.09 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.10 nm/sec Defocus = -3.279 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_036_26.0_Oct22_20.19.31.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 99.7 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.174 0.214 IS units -752.8 270.6 unbinned pixels; need stage -0.070 -0.306 if reset Tilting to 28 Correcting tilt backlash -5.9 -2.4 unbinned pixels; 0.1 2.2 nm along two shift axes; 1.0 -2.0 nm on specimen axes Rate = 11.21732 A/sec -1.8 5.0 unbinned pixels; 1.9 0.0 nm along two shift axes; -1.7 -0.8 nm on specimen axes Rate = 9.42897 A/sec 1.5 2.1 unbinned pixels; 0.5 0.8 nm along two shift axes; -0.8 0.5 nm on specimen axes Rate = 4.63788 A/sec Drift is low enough after shot 3 Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.05 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.06 nm/sec Defocus = -3.335 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_037_28.0_Oct22_20.20.47.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 90.5 In SetExposureForMean 90, change by a factor of 0.9964 would require too small a change in exposure time Image shift -0.125 0.102 IS units -399.0 242.1 unbinned pixels; need stage -0.071 -0.169 if reset Tilting to -48 -48.00 degrees Trimming A by 20 65, reference by 25 60 Trimming A by 20 60, reference by 25 60 6.1 -7.4 unbinned pixels; 3.2 1.1 nm along two shift axes; 2.4 2.4 nm on specimen axes Rate = 16.8193 A/sec Trimming A by 20 60, reference by 25 60 4.0 2.7 unbinned pixels; 0.4 1.6 nm along two shift axes; -1.1 1.3 nm on specimen axes Rate = 8.51028 A/sec Trimming A by 20 60, reference by 25 60 0.9 0.4 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.65166 A/sec Drift is low enough after shot 3 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.06 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.289 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_038_-48.0_Oct22_20.22.03.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 80.2 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift 0.240 -0.719 IS units 2183.1 30.2 unbinned pixels; need stage -0.078 1.152 if reset Tilting to -50 Trimming A by 22 75, reference by 25 60 Trimming A by 20 60, reference by 25 60 -1.1 -5.3 unbinned pixels; 1.6 1.0 nm along two shift axes; 1.9 -0.2 nm on specimen axes Rate = 9.60866 A/sec Trimming A by 20 60, reference by 25 60 1.9 2.2 unbinned pixels; 0.5 0.9 nm along two shift axes; -0.8 0.6 nm on specimen axes Rate = 5.11645 A/sec Drift is low enough after shot 2 Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.03 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.287 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_039_-50.0_Oct22_20.23.07.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 87.4 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift 0.270 -0.765 IS units 2339.4 -6.4 unbinned pixels; need stage -0.070 1.287 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -14.3 3.5 unbinned pixels; 2.8 4.3 nm along two shift axes; -0.8 -5.1 nm on specimen axes Rate = 25.86657 A/sec -10.3 3.4 unbinned pixels; 2.3 3.0 nm along two shift axes; -0.9 -3.7 nm on specimen axes Rate = 19.02999 A/sec -8.1 3.1 unbinned pixels; 2.0 2.3 nm along two shift axes; -0.8 -2.9 nm on specimen axes Rate = 15.22434 A/sec -5.9 2.2 unbinned pixels; 1.4 1.7 nm along two shift axes; -0.6 -2.1 nm on specimen axes Rate = 11.0323 A/sec -5.2 1.9 unbinned pixels; 1.2 1.5 nm along two shift axes; -0.5 -1.9 nm on specimen axes Rate = 9.65945 A/sec -3.9 1.8 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.5 -1.4 nm on specimen axes Rate = 7.62 A/sec Drift is low enough after shot 6 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.13 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.11 nm/sec Defocus = -3.506 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_040_30.0_Oct22_20.24.40.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 106.5 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.145 0.167 IS units -595.1 235.2 unbinned pixels; need stage -0.063 -0.252 if reset Tilting to 32 Correcting tilt backlash -9.2 -0.5 unbinned pixels; 0.9 3.1 nm along two shift axes; 0.4 -3.2 nm on specimen axes Rate = 16.12315 A/sec -1.8 5.4 unbinned pixels; 2.0 0.0 nm along two shift axes; -1.8 -0.8 nm on specimen axes Rate = 9.97171 A/sec 0.8 3.9 unbinned pixels; 1.2 0.7 nm along two shift axes; -1.4 0.1 nm on specimen axes Rate = 7.05114 A/sec Drift is low enough after shot 3 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.08 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -3.518 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_041_32.0_Oct22_20.25.51.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 84.5 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.107 0.069 IS units -291.8 225.5 unbinned pixels; need stage -0.069 -0.130 if reset Tilting to -52 -52.00 degrees Trimming A by 20 70, reference by 21 65 Trimming A by 20 65, reference by 21 65 6.5 -10.9 unbinned pixels; 4.4 0.8 nm along two shift axes; 3.6 2.6 nm on specimen axes Rate = 22.27284 A/sec Trimming A by 20 60, reference by 21 65 4.3 3.8 unbinned pixels; 0.8 1.9 nm along two shift axes; -1.5 1.4 nm on specimen axes Rate = 10.14453 A/sec Trimming A by 20 65, reference by 21 65 1.1 1.3 unbinned pixels; 0.3 0.5 nm along two shift axes; -0.5 0.3 nm on specimen axes Rate = 2.9702 A/sec Drift is low enough after shot 3 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.03 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -0.219 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_042_-52.0_Oct22_20.27.06.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 78.1 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift 0.339 -0.932 IS units 2860.0 -36.6 unbinned pixels; need stage -0.075 1.644 if reset Tilting to -54 Trimming A by 20 70, reference by 20 65 Trimming A by 21 65, reference by 20 65 2.5 -4.3 unbinned pixels; 1.7 0.3 nm along two shift axes; 1.4 1.0 nm on specimen axes Rate = 8.78172 A/sec Trimming A by 21 65, reference by 20 65 0.4 2.5 unbinned pixels; 0.8 0.4 nm along two shift axes; -0.9 0.1 nm on specimen axes Rate = 4.38404 A/sec Drift is low enough after shot 2 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.03 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.06 nm/sec Defocus = -0.211 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_043_-54.0_Oct22_20.28.11.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 83.7 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift 0.360 -0.968 IS units 2976.6 -60.4 unbinned pixels; need stage -0.071 1.794 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -19.7 7.4 unbinned pixels; 4.8 5.7 nm along two shift axes; -2.0 -7.1 nm on specimen axes Rate = 36.92069 A/sec -16.9 6.3 unbinned pixels; 4.0 4.8 nm along two shift axes; -1.7 -6.1 nm on specimen axes Rate = 31.55604 A/sec -9.2 5.9 unbinned pixels; 3.0 2.3 nm along two shift axes; -1.8 -3.4 nm on specimen axes Rate = 19.14514 A/sec -8.1 4.3 unbinned pixels; 2.4 2.2 nm along two shift axes; -1.3 -3.0 nm on specimen axes Rate = 16.07137 A/sec -4.0 3.4 unbinned pixels; 1.6 0.9 nm along two shift axes; -1.1 -1.5 nm on specimen axes Rate = 9.186 A/sec -4.5 3.0 unbinned pixels; 1.5 1.1 nm along two shift axes; -0.9 -1.7 nm on specimen axes Rate = 9.49154 A/sec -4.9 2.0 unbinned pixels; 1.2 1.4 nm along two shift axes; -0.5 -1.8 nm on specimen axes Rate = 9.21116 A/sec -3.5 1.5 unbinned pixels; 0.9 1.0 nm along two shift axes; -0.4 -1.3 nm on specimen axes Rate = 6.68029 A/sec Drift is low enough after shot 8 Measured defocus = -4.37 microns changed by -0.13 to target drift = 0.14 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -3.640 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_044_34.0_Oct22_20.29.57.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 104.6 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.116 0.106 IS units -400.7 214.7 unbinned pixels; need stage -0.062 -0.179 if reset Tilting to 36 Correcting tilt backlash 5.6 -5.3 unbinned pixels; 2.4 1.2 nm along two shift axes; 1.7 2.1 nm on specimen axes Rate = 13.57245 A/sec 2.8 4.9 unbinned pixels; 1.3 1.5 nm along two shift axes; -1.8 0.8 nm on specimen axes Rate = 9.92121 A/sec 4.5 0.8 unbinned pixels; 0.3 1.6 nm along two shift axes; -0.4 1.6 nm on specimen axes Rate = 8.021 A/sec 0.2 1.8 unbinned pixels; 0.6 0.3 nm along two shift axes; -0.6 0.0 nm on specimen axes Rate = 3.14583 A/sec Drift is low enough after shot 4 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.04 nm/sec Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.08 nm/sec Defocus = -3.707 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_045_36.0_Oct22_20.31.19.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 89.1 In SetExposureForMean 90, change by a factor of 1.0122 would require too small a change in exposure time Image shift -0.076 0.025 IS units -142.5 182.2 unbinned pixels; need stage -0.058 -0.070 if reset Tilting to -56 -56.00 degrees Trimming A by 20 70, reference by 20 65 Trimming A by 21 65, reference by 20 65 12.0 -6.3 unbinned pixels; 3.5 3.2 nm along two shift axes; 1.8 4.4 nm on specimen axes Rate = 23.70428 A/sec Trimming A by 25 60, reference by 20 65 1.8 2.0 unbinned pixels; 0.5 0.8 nm along two shift axes; -0.8 0.6 nm on specimen axes Rate = 4.73726 A/sec Drift is low enough after shot 2 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.08 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = -0.138 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_046_-56.0_Oct22_20.32.30.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 74.0 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift 0.400 -1.089 IS units 3344.8 -53.7 unbinned pixels; need stage -0.084 2.118 if reset Tilting to -58 Trimming A by 20 75, reference by 26 65 Trimming A by 21 65, reference by 26 65 -1.8 -6.2 unbinned pixels; 1.8 1.3 nm along two shift axes; 2.2 -0.4 nm on specimen axes Rate = 11.34395 A/sec Trimming A by 21 65, reference by 26 65 3.8 2.4 unbinned pixels; 0.4 1.6 nm along two shift axes; -1.0 1.3 nm on specimen axes Rate = 7.96927 A/sec Drift is low enough after shot 2 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.03 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -0.164 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_047_-58.0_Oct22_20.33.34.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 86.5 In SetExposureForMean 90, exposure time changed to 3.3600 Image shift 0.450 -1.181 IS units 3642.1 -102.7 unbinned pixels; need stage -0.077 2.436 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -15.2 10.1 unbinned pixels; 5.1 3.8 nm along two shift axes; -3.1 -5.6 nm on specimen axes Rate = 31.99444 A/sec -4.4 8.2 unbinned pixels; 3.2 0.5 nm along two shift axes; -2.7 -1.8 nm on specimen axes Rate = 16.35839 A/sec -1.6 3.0 unbinned pixels; 1.2 0.2 nm along two shift axes; -1.0 -0.7 nm on specimen axes Rate = 5.93521 A/sec Drift is low enough after shot 3 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.08 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -3.831 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_048_38.0_Oct22_20.34.53.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 111.3 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift -0.113 0.066 IS units -289.1 244.3 unbinned pixels; need stage -0.075 -0.140 if reset Tilting to 40 Correcting tilt backlash Trimming A by 25 40, reference by 20 20 -2.0 -5.2 unbinned pixels; 1.5 1.3 nm along two shift axes; 1.9 -0.6 nm on specimen axes Rate = 9.93648 A/sec -4.1 4.6 unbinned pixels; 2.0 0.8 nm along two shift axes; -1.5 -1.6 nm on specimen axes Rate = 10.83427 A/sec -1.6 2.0 unbinned pixels; 0.8 0.3 nm along two shift axes; -0.6 -0.6 nm on specimen axes Rate = 4.45493 A/sec Drift is low enough after shot 3 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.09 nm/sec Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.01 nm/sec Defocus = -3.798 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_049_40.0_Oct22_20.36.05.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 85.3 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift -0.052 -0.042 IS units 64.1 181.1 unbinned pixels; need stage -0.064 0.022 if reset Tilting to -60 -60.00 degrees Trimming A by 20 70, reference by 21 65 Trimming A by 20 65, reference by 21 65 6.8 -6.6 unbinned pixels; 3.0 1.5 nm along two shift axes; 2.1 2.6 nm on specimen axes Rate = 16.56412 A/sec Trimming A by 21 65, reference by 21 65 3.8 0.5 unbinned pixels; 0.3 1.3 nm along two shift axes; -0.3 1.3 nm on specimen axes Rate = 6.79804 A/sec Drift is low enough after shot 2 Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.08 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.02 nm/sec Defocus = -0.122 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_050_-60.0_Oct22_20.37.16.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 70.4 In SetExposureForMean 90, exposure time changed to 3.6800 Image shift 0.504 -1.300 IS units 4018.1 -134.4 unbinned pixels; need stage -0.077 2.850 if reset Tilting to -62 Trimming A by 20 70, reference by 22 70 Trimming A by 25 65, reference by 22 70 2.5 -5.6 unbinned pixels; 2.1 0.2 nm along two shift axes; 1.9 1.1 nm on specimen axes Rate = 10.77493 A/sec Trimming A by 20 65, reference by 22 70 0.5 2.8 unbinned pixels; 0.9 0.5 nm along two shift axes; -1.0 0.1 nm on specimen axes Rate = 4.9602 A/sec Drift is low enough after shot 2 Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -0.160 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_051_-62.0_Oct22_20.38.20.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 79.2 In SetExposureForMean 90, exposure time changed to 4.0000 Trimming A by 75 75, reference by 20 20 Image shift 0.546 -1.384 IS units 4287.6 -169.8 unbinned pixels; need stage -0.073 3.241 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -11.8 2.9 unbinned pixels; 2.3 3.6 nm along two shift axes; -0.6 -4.2 nm on specimen axes Rate = 21.26583 A/sec -9.2 2.9 unbinned pixels; 2.0 2.7 nm along two shift axes; -0.7 -3.3 nm on specimen axes Rate = 16.90265 A/sec -8.2 2.7 unbinned pixels; 1.9 2.4 nm along two shift axes; -0.7 -3.0 nm on specimen axes Rate = 15.14475 A/sec -6.1 1.5 unbinned pixels; 1.2 1.8 nm along two shift axes; -0.3 -2.2 nm on specimen axes Rate = 10.98733 A/sec -3.5 0.6 unbinned pixels; 0.6 1.1 nm along two shift axes; -0.1 -1.2 nm on specimen axes Rate = 6.23826 A/sec Drift is low enough after shot 5 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.16 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.04 nm/sec Defocus = -3.887 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_052_42.0_Oct22_20.39.56.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 118.7 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift -0.060 -0.038 IS units 42.9 199.6 unbinned pixels; need stage -0.070 0.012 if reset Tilting to 44 Correcting tilt backlash Trimming A by 30 30, reference by 20 20 -9.3 -2.6 unbinned pixels; 0.2 3.4 nm along two shift axes; 1.2 -3.2 nm on specimen axes Rate = 17.02037 A/sec -3.3 4.1 unbinned pixels; 1.7 0.6 nm along two shift axes; -1.3 -1.3 nm on specimen axes Rate = 9.16592 A/sec 1.9 2.8 unbinned pixels; 0.7 0.9 nm along two shift axes; -1.0 0.6 nm on specimen axes Rate = 5.9124 A/sec Drift is low enough after shot 3 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.02 nm/sec Defocus = -3.855 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_053_44.0_Oct22_20.41.07.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 84.3 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift -0.017 -0.113 IS units 288.6 153.1 unbinned pixels; need stage -0.062 0.135 if reset Tilting to -64 -64.00 degrees Trimming A by 20 70, reference by 20 65 Trimming A by 21 65, reference by 20 65 6.4 -5.8 unbinned pixels; 2.7 1.4 nm along two shift axes; 1.8 2.4 nm on specimen axes Rate = 15.2078 A/sec Trimming A by 20 65, reference by 20 65 4.6 1.6 unbinned pixels; 0.0 1.7 nm along two shift axes; -0.7 1.6 nm on specimen axes Rate = 8.54487 A/sec Trimming A by 21 65, reference by 20 65 2.3 -0.6 unbinned pixels; 0.5 0.7 nm along two shift axes; 0.2 0.8 nm on specimen axes Rate = 4.16923 A/sec Drift is low enough after shot 3 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.12 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.076 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_054_-64.0_Oct22_20.42.24.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 58.5 In SetExposureForMean 90, exposure time changed to 4.4800 Trimming A by 110 115, reference by 80 80 Image shift 0.585 -1.463 IS units 4540.6 -200.7 unbinned pixels; need stage -0.071 3.677 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash Trimming A by 20 25, reference by 20 20 -15.9 4.0 unbinned pixels; 3.2 4.8 nm along two shift axes; -0.9 -5.7 nm on specimen axes Rate = 28.83086 A/sec -7.2 3.6 unbinned pixels; 2.0 2.0 nm along two shift axes; -1.0 -2.7 nm on specimen axes Rate = 14.1509 A/sec -7.6 2.6 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.7 -2.7 nm on specimen axes Rate = 14.07504 A/sec Trimming A by 20 25, reference by 20 20 -3.1 0.6 unbinned pixels; 0.6 1.0 nm along two shift axes; -0.1 -1.1 nm on specimen axes Rate = 5.58567 A/sec Drift is low enough after shot 4 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.06 nm/sec Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.03 nm/sec Defocus = -3.997 um Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_055_46.0_Oct22_20.43.49.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 115.5 In SetExposureForMean 90, exposure time changed to 3.5200 Image shift -0.039 -0.095 IS units 218.3 196.7 unbinned pixels; need stage -0.074 0.102 if reset Tilting to 48 Correcting tilt backlash Trimming A by 20 50, reference by 25 25 Trimming A by 25 40, reference by 25 25 -5.0 -2.0 unbinned pixels; 0.1 1.9 nm along two shift axes; 0.9 -1.7 nm on specimen axes Rate = 9.51838 A/sec Trimming A by 20 40, reference by 25 25 2.6 3.7 unbinned pixels; 0.9 1.3 nm along two shift axes; -1.4 0.8 nm on specimen axes Rate = 7.9289 A/sec Drift is low enough after shot 2 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.14 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.15 nm/sec Defocus = -3.994 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_01_1_056_48.0_Oct22_20.44.57.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 83.5 In SetExposureForMean 90, exposure time changed to 3.8400 Image shift 0.016 -0.187 IS units 522.1 134.5 unbinned pixels; need stage -0.062 0.269 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -2.96 microns changed by -1.54 to target drift = 0.07 nm/sec Focus area changed from stored state, axis position -2.60 angle 0, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_02.st Running script 1 at item 4 (119.57, -135.25) 20:45:51 Refine ZLP: additional offset = -0.5 eV; total offset = 5.7 eV Item 4: Stage: 119.57 -135.25 -235 Label: 4 Note: Sec 2 - anchor_maps.map Target defocus is: -4.50 um Target defocus is: -4.75 um Target defocus = -4.75 Angle = -4.40, increment = 0.60, shift = -9.3 -5.9 pixels, -0.023 um Y Angle = -3.20, increment = 1.80, shift = -32.8 -9.2 pixels, -0.077 um Y Angle = 0.41, increment = 5.41, shift = -91.6 -18.1 pixels, -0.212 um Y Angle = 3.00, increment = 8.00, shift = -117.7 -24.1 pixels, -0.273 um Y Rough eucentricity: changing Z by 1.96 to -232.98, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = -19.7, 6.1 pixels, 0.019, -0.043 um, IS = -0.019, 0.043 Angle = 11.00, shift = 14.8, 3.8 pixels, 0.005, 0.035 um, IS = -0.024, 0.009 Angle = 4.00, shift = 24.1, 7.9 pixels, 0.012, 0.057 um, IS = -0.035, -0.048 Angle = -4.00, shift = -26.7, -25.2 pixels, -0.050, -0.067 um, IS = 0.015, 0.019 Trimming A by 20 25, reference by 20 20 Angle = -11.00, shift = 11.0, 2.3 pixels, 0.002, 0.025 um, IS = 0.012, -0.007 Angle = -18.00, shift = 78.9, 14.7 pixels, 0.013, 0.183 um, IS = -0.001, -0.190 Angle = -24.00, shift = 73.4, 17.8 pixels, 0.022, 0.171 um, IS = -0.022, -0.361 General solution delZ -0.36, delY 2.11, yZero 0.02 solution with assumed YZero -0.01: delZ -0.36, delY 1.67 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.36 microns and lateral displacement of -2.11 microns Disparity in stage position after aligning to center of map frame: -0.04 -0.74 Aligning to target with image shift equivalent to stage shift: -0.05 -0.75 Disparity in stage position after aligning to map item itself: -0.05 -0.68 (align shift -0.00 0.02) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 119.58 -135.21 -232.61 -0.5 1.0 unbinned pixels; 0.4 0.0 nm along two shift axes; -0.3 -0.2 nm on specimen axes Rate = 1.9788 A/sec Drift is low enough after shot 1 Measured defocus = -3.02 microns changed by -1.73 to target drift = 0.05 nm/sec Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.03 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.783 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_000_-10.0_Oct22_20.49.45.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 85.5 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.343 0.608 IS units -1985.0 354.8 unbinned pixels; need stage -0.061 -0.724 if reset Tilting to -8 Correcting tilt backlash 7.7 0.1 unbinned pixels; 0.9 2.6 nm along two shift axes; -0.3 2.7 nm on specimen axes Rate = 13.51759 A/sec 5.8 1.2 unbinned pixels; 0.3 2.1 nm along two shift axes; -0.6 2.0 nm on specimen axes Rate = 10.37419 A/sec 2.6 0.4 unbinned pixels; 0.2 0.9 nm along two shift axes; -0.2 0.9 nm on specimen axes Rate = 4.58135 A/sec Drift is low enough after shot 3 Measured defocus = -4.62 microns changed by -0.13 to target drift = 0.06 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -1.912 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_001_-8.0_Oct22_20.50.54.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 94.9 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.389 0.635 IS units -2103.9 453.8 unbinned pixels; need stage -0.091 -0.766 if reset Tilting to -12 -12.00 degrees -5.0 0.7 unbinned pixels; 0.8 1.6 nm along two shift axes; -0.1 -1.8 nm on specimen axes Rate = 8.86279 A/sec -1.4 5.0 unbinned pixels; 1.8 0.1 nm along two shift axes; -1.7 -0.6 nm on specimen axes Rate = 9.15118 A/sec -1.6 0.7 unbinned pixels; 0.4 0.5 nm along two shift axes; -0.2 -0.6 nm on specimen axes Rate = 3.12888 A/sec Drift is low enough after shot 3 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.07 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.706 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_002_-12.0_Oct22_20.52.07.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 85.0 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.420 0.688 IS units -2278.6 488.3 unbinned pixels; need stage -0.098 -0.840 if reset Tilting to -14 2.6 -0.3 unbinned pixels; 0.4 0.8 nm along two shift axes; 0.0 0.9 nm on specimen axes Rate = 4.49992 A/sec Drift is low enough after shot 1 Measured defocus = -4.88 microns changed by 0.13 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -1.564 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_003_-14.0_Oct22_20.53.02.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 92.6 In SetExposureForMean 90, change by a factor of 0.9724 would require too small a change in exposure time Image shift -0.449 0.746 IS units -2462.4 511.9 unbinned pixels; need stage -0.100 -0.914 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 8.7 -6.8 unbinned pixels; 3.3 2.1 nm along two shift axes; 2.1 3.3 nm on specimen axes Rate = 19.35227 A/sec 5.4 0.9 unbinned pixels; 0.3 1.9 nm along two shift axes; -0.5 1.9 nm on specimen axes Rate = 9.60823 A/sec 3.0 0.6 unbinned pixels; 0.1 1.1 nm along two shift axes; -0.3 1.0 nm on specimen axes Rate = 5.4093 A/sec Drift is low enough after shot 3 Measured defocus = -4.63 microns changed by -0.12 to target drift = 0.07 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -2.039 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_004_-6.0_Oct22_20.54.12.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 95.1 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.347 0.544 IS units -1815.0 427.4 unbinned pixels; need stage -0.091 -0.659 if reset Tilting to -4 Correcting tilt backlash -3.9 -6.2 unbinned pixels; 1.6 2.0 nm along two shift axes; 2.3 -1.2 nm on specimen axes Rate = 12.86052 A/sec -1.5 2.0 unbinned pixels; 0.8 0.2 nm along two shift axes; -0.7 -0.6 nm on specimen axes Rate = 4.39756 A/sec Drift is low enough after shot 2 Measured defocus = -4.61 microns changed by -0.14 to target drift = 0.04 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.02 nm/sec Defocus = -2.180 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_005_-4.0_Oct22_20.55.16.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 86.7 In SetExposureForMean 90, change by a factor of 1.0376 would require too small a change in exposure time Image shift -0.304 0.463 IS units -1554.3 386.4 unbinned pixels; need stage -0.085 -0.563 if reset Tilting to -16 -16.00 degrees -7.5 -6.1 unbinned pixels; 1.1 3.2 nm along two shift axes; 2.4 -2.4 nm on specimen axes Rate = 16.98063 A/sec -1.8 2.5 unbinned pixels; 1.1 0.3 nm along two shift axes; -0.8 -0.7 nm on specimen axes Rate = 5.46612 A/sec Drift is low enough after shot 2 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.484 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_006_-16.0_Oct22_20.56.19.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 83.4 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.403 0.631 IS units -2106.5 496.2 unbinned pixels; need stage -0.106 -0.792 if reset Tilting to -18 2.8 -2.2 unbinned pixels; 1.0 0.7 nm along two shift axes; 0.7 1.0 nm on specimen axes Rate = 6.14512 A/sec Drift is low enough after shot 1 Measured defocus = -4.87 microns changed by 0.12 to target drift = 0.08 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -1.356 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_007_-18.0_Oct22_20.57.15.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 90.4 In SetExposureForMean 90, change by a factor of 0.9949 would require too small a change in exposure time Image shift -0.380 0.594 IS units -1983.6 468.1 unbinned pixels; need stage -0.100 -0.753 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 10.0 0.3 unbinned pixels; 1.1 3.3 nm along two shift axes; -0.4 3.5 nm on specimen axes Rate = 17.57484 A/sec 6.2 -0.6 unbinned pixels; 0.9 2.0 nm along two shift axes; 0.0 2.2 nm on specimen axes Rate = 10.9468 A/sec 3.2 -0.1 unbinned pixels; 0.4 1.1 nm along two shift axes; -0.1 1.1 nm on specimen axes Rate = 5.69252 A/sec Drift is low enough after shot 3 Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.06 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -2.240 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_008_-2.0_Oct22_20.58.26.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 95.6 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.315 0.466 IS units -1573.6 413.7 unbinned pixels; need stage -0.094 -0.570 if reset Tilting to 0 Correcting tilt backlash -6.5 -4.5 unbinned pixels; 0.7 2.7 nm along two shift axes; 1.8 -2.1 nm on specimen axes Rate = 13.99378 A/sec -2.0 1.7 unbinned pixels; 0.8 0.5 nm along two shift axes; -0.5 -0.8 nm on specimen axes Rate = 4.55295 A/sec Drift is low enough after shot 2 Measured defocus = -4.68 microns changed by -0.07 to target drift = 0.06 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -2.311 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_009_-0.0_Oct22_20.59.29.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 87.0 In SetExposureForMean 90, change by a factor of 1.0343 would require too small a change in exposure time Image shift -0.284 0.419 IS units -1415.8 373.2 unbinned pixels; need stage -0.085 -0.513 if reset Tilting to -20 -20.00 degrees -4.3 -6.6 unbinned pixels; 1.7 2.2 nm along two shift axes; 2.4 -1.3 nm on specimen axes Rate = 13.85587 A/sec 0.0 2.3 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.8 -0.1 nm on specimen axes Rate = 3.99089 A/sec Drift is low enough after shot 2 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.02 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -1.284 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_010_-20.0_Oct22_21.00.38.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 80.9 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.324 0.464 IS units -1576.5 439.4 unbinned pixels; need stage -0.103 -0.608 if reset Tilting to -22 3.5 -0.7 unbinned pixels; 0.7 1.1 nm along two shift axes; 0.1 1.3 nm on specimen axes Rate = 6.31361 A/sec Drift is low enough after shot 1 Measured defocus = -4.84 microns changed by 0.09 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.181 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_011_-22.0_Oct22_21.01.33.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 87.6 In SetExposureForMean 90, change by a factor of 1.0263 would require too small a change in exposure time Image shift -0.310 0.443 IS units -1505.5 421.5 unbinned pixels; need stage -0.099 -0.589 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 8.2 -0.3 unbinned pixels; 1.1 2.7 nm along two shift axes; -0.1 2.9 nm on specimen axes Rate = 14.36357 A/sec 4.5 -0.1 unbinned pixels; 0.5 1.5 nm along two shift axes; -0.1 1.6 nm on specimen axes Rate = 7.88302 A/sec Drift is low enough after shot 2 Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -2.373 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_012_2.0_Oct22_21.02.39.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 95.5 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.290 0.404 IS units -1379.8 406.5 unbinned pixels; need stage -0.097 -0.501 if reset Tilting to 4 Correcting tilt backlash -14.3 1.3 unbinned pixels; 2.1 4.6 nm along two shift axes; -0.0 -5.1 nm on specimen axes Rate = 25.20071 A/sec -10.5 2.2 unbinned pixels; 1.9 3.2 nm along two shift axes; -0.4 -3.7 nm on specimen axes Rate = 18.71846 A/sec -8.1 1.6 unbinned pixels; 1.5 2.5 nm along two shift axes; -0.3 -2.9 nm on specimen axes Rate = 14.39777 A/sec -6.2 1.3 unbinned pixels; 1.2 1.9 nm along two shift axes; -0.3 -2.2 nm on specimen axes Rate = 11.03042 A/sec -4.6 1.2 unbinned pixels; 0.9 1.4 nm along two shift axes; -0.3 -1.7 nm on specimen axes Rate = 8.38403 A/sec -4.2 1.1 unbinned pixels; 0.9 1.3 nm along two shift axes; -0.3 -1.5 nm on specimen axes Rate = 7.57915 A/sec Drift is low enough after shot 6 Measured defocus = -4.67 microns changed by -0.08 to target drift = 0.11 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -2.449 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_013_4.0_Oct22_21.04.05.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 86.8 In SetExposureForMean 90, change by a factor of 1.0367 would require too small a change in exposure time Image shift -0.291 0.414 IS units -1409.2 397.7 unbinned pixels; need stage -0.093 -0.512 if reset Tilting to -24 -24.00 degrees -2.7 -6.9 unbinned pixels; 2.0 1.7 nm along two shift axes; 2.5 -0.7 nm on specimen axes Rate = 13.04419 A/sec 0.3 2.0 unbinned pixels; 0.6 0.3 nm along two shift axes; -0.7 0.1 nm on specimen axes Rate = 3.52906 A/sec Drift is low enough after shot 2 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.01 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.01 nm/sec Defocus = -1.111 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_014_-24.0_Oct22_21.05.09.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 78.0 In SetExposureForMean 90, exposure time changed to 1.9200 Trimming A by 30 35, reference by 20 20 Image shift -0.253 0.303 IS units -1069.4 399.9 unbinned pixels; need stage -0.105 -0.428 if reset Tilting to -26 3.8 0.3 unbinned pixels; 0.3 1.3 nm along two shift axes; -0.2 1.3 nm on specimen axes Rate = 6.74171 A/sec Drift is low enough after shot 1 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.035 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_015_-26.0_Oct22_21.06.04.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 91.7 In SetExposureForMean 90, change by a factor of 0.9793 would require too small a change in exposure time Trimming A by 30 35, reference by 30 35 Image shift -0.248 0.297 IS units -1049.1 392.6 unbinned pixels; need stage -0.103 -0.427 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 5.7 0.1 unbinned pixels; 0.6 1.9 nm along two shift axes; -0.2 2.0 nm on specimen axes Rate = 9.92023 A/sec 2.7 0.7 unbinned pixels; 0.1 1.0 nm along two shift axes; -0.3 0.9 nm on specimen axes Rate = 4.91488 A/sec Drift is low enough after shot 2 Measured defocus = -4.65 microns changed by -0.10 to target drift = 0.07 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -2.547 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_016_6.0_Oct22_21.07.10.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 103.9 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.295 0.397 IS units -1366.6 425.9 unbinned pixels; need stage -0.104 -0.500 if reset Tilting to 8 Correcting tilt backlash -19.8 4.8 unbinned pixels; 3.9 6.0 nm along two shift axes; -1.1 -7.1 nm on specimen axes Rate = 35.71674 A/sec -14.8 3.8 unbinned pixels; 3.0 4.4 nm along two shift axes; -0.9 -5.3 nm on specimen axes Rate = 26.72521 A/sec -9.9 2.8 unbinned pixels; 2.1 2.9 nm along two shift axes; -0.7 -3.6 nm on specimen axes Rate = 18.02121 A/sec -7.5 2.2 unbinned pixels; 1.6 2.2 nm along two shift axes; -0.5 -2.7 nm on specimen axes Rate = 13.76094 A/sec -6.3 1.9 unbinned pixels; 1.4 1.9 nm along two shift axes; -0.5 -2.3 nm on specimen axes Rate = 11.5179 A/sec -4.6 1.6 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.50969 A/sec -4.4 1.6 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.12383 A/sec -3.7 1.3 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.3 -1.3 nm on specimen axes Rate = 6.83077 A/sec Drift is low enough after shot 8 Measured defocus = -4.66 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.643 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_017_8.0_Oct22_21.08.47.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 86.2 In SetExposureForMean 90, change by a factor of 1.0444 would require too small a change in exposure time Image shift -0.308 0.422 IS units -1446.1 437.5 unbinned pixels; need stage -0.106 -0.531 if reset Tilting to -28 -28.00 degrees 0.1 -4.2 unbinned pixels; 1.4 0.5 nm along two shift axes; 1.5 0.2 nm on specimen axes Rate = 7.42604 A/sec Drift is low enough after shot 1 Measured defocus = -4.81 microns changed by 0.06 to target drift = 0.02 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.969 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_018_-28.0_Oct22_21.09.47.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 74.8 In SetExposureForMean 90, exposure time changed to 1.9200 Trimming A by 30 40, reference by 30 35 Image shift -0.210 0.186 IS units -711.9 394.1 unbinned pixels; need stage -0.114 -0.300 if reset Tilting to -30 1.3 -4.1 unbinned pixels; 1.5 0.0 nm along two shift axes; 1.4 0.6 nm on specimen axes Rate = 7.50539 A/sec Drift is low enough after shot 1 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.07 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.924 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_019_-30.0_Oct22_21.10.43.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 87.7 In SetExposureForMean 90, change by a factor of 1.0213 would require too small a change in exposure time Trimming A by 20 50, reference by 21 50 Image shift -0.178 0.117 IS units -492.2 373.0 unbinned pixels; need stage -0.113 -0.215 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees 0.3 3.6 unbinned pixels; 1.2 0.5 nm along two shift axes; -1.3 -0.0 nm on specimen axes Rate = 6.29401 A/sec Drift is low enough after shot 1 Measured defocus = -4.65 microns changed by -0.10 to target drift = 0.07 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.750 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_020_10.0_Oct22_21.11.45.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 102.8 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.327 0.429 IS units -1483.3 481.2 unbinned pixels; need stage -0.120 -0.548 if reset Tilting to 12 Correcting tilt backlash -13.1 4.3 unbinned pixels; 2.9 3.8 nm along two shift axes; -1.1 -4.7 nm on specimen axes Rate = 24.18858 A/sec -7.7 2.8 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.8 -2.8 nm on specimen axes Rate = 14.31901 A/sec -5.8 2.5 unbinned pixels; 1.5 1.6 nm along two shift axes; -0.7 -2.1 nm on specimen axes Rate = 11.12381 A/sec -3.6 1.8 unbinned pixels; 1.0 1.0 nm along two shift axes; -0.5 -1.3 nm on specimen axes Rate = 7.09857 A/sec Drift is low enough after shot 4 Measured defocus = -4.63 microns changed by -0.12 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.872 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_021_12.0_Oct22_21.13.00.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 93.9 In SetExposureForMean 90, change by a factor of 0.9587 would require too small a change in exposure time Image shift -0.327 0.433 IS units -1494.7 476.9 unbinned pixels; need stage -0.118 -0.556 if reset Tilting to -32 -32.00 degrees 0.5 -3.0 unbinned pixels; 1.1 0.2 nm along two shift axes; 1.1 0.3 nm on specimen axes Rate = 5.45348 A/sec Drift is low enough after shot 1 Measured defocus = -4.80 microns changed by 0.05 to target drift = 0.05 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -0.881 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_022_-32.0_Oct22_21.14.00.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 78.1 In SetExposureForMean 90, exposure time changed to 2.0800 Trimming A by 22 65, reference by 20 50 Image shift -0.151 0.008 IS units -171.5 403.6 unbinned pixels; need stage -0.134 -0.087 if reset Tilting to -34 1.2 -0.0 unbinned pixels; 0.2 0.4 nm along two shift axes; -0.0 0.4 nm on specimen axes Rate = 2.13966 A/sec Drift is low enough after shot 1 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.03 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.832 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_023_-34.0_Oct22_21.14.56.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 89.6 In SetExposureForMean 90, change by a factor of 0.9979 would require too small a change in exposure time Trimming A by 20 55, reference by 21 65 Image shift -0.136 -0.021 IS units -76.8 390.4 unbinned pixels; need stage -0.132 -0.048 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -7.2 3.6 unbinned pixels; 2.0 1.9 nm along two shift axes; -1.0 -2.6 nm on specimen axes Rate = 14.07784 A/sec -4.5 2.9 unbinned pixels; 1.5 1.1 nm along two shift axes; -0.9 -1.7 nm on specimen axes Rate = 9.30906 A/sec -3.0 1.4 unbinned pixels; 0.8 0.8 nm along two shift axes; -0.4 -1.1 nm on specimen axes Rate = 5.87222 A/sec Drift is low enough after shot 3 Measured defocus = -4.62 microns changed by -0.13 to target drift = 0.08 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -3.006 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_024_14.0_Oct22_21.16.09.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 110.1 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.339 0.429 IS units -1496.5 515.3 unbinned pixels; need stage -0.131 -0.563 if reset Tilting to 16 Correcting tilt backlash -0.4 -2.6 unbinned pixels; 0.8 0.5 nm along two shift axes; 0.9 -0.1 nm on specimen axes Rate = 4.7199 A/sec Drift is low enough after shot 1 Measured defocus = -4.65 microns changed by -0.10 to target drift = 0.09 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -3.115 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_025_16.0_Oct22_21.17.07.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 92.1 In SetExposureForMean 90, change by a factor of 0.9774 would require too small a change in exposure time Image shift -0.298 0.348 IS units -1235.9 479.0 unbinned pixels; need stage -0.127 -0.471 if reset Tilting to -36 -36.00 degrees -1.2 -1.2 unbinned pixels; 0.2 0.5 nm along two shift axes; 0.4 -0.4 nm on specimen axes Rate = 2.9883 A/sec Drift is low enough after shot 1 Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.03 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.805 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_026_-36.0_Oct22_21.18.07.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 73.5 In SetExposureForMean 90, exposure time changed to 2.0800 Trimming A by 20 60, reference by 20 55 Image shift -0.112 -0.116 IS units 205.0 416.1 unbinned pixels; need stage -0.149 0.073 if reset Tilting to -38 2.0 -4.1 unbinned pixels; 1.6 0.2 nm along two shift axes; 1.4 0.8 nm on specimen axes Rate = 8.0358 A/sec 4.6 1.8 unbinned pixels; 0.1 1.7 nm along two shift axes; -0.8 1.5 nm on specimen axes Rate = 8.64375 A/sec 1.6 0.6 unbinned pixels; 0.0 0.6 nm along two shift axes; -0.3 0.6 nm on specimen axes Rate = 3.0809 A/sec Drift is low enough after shot 3 Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.05 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.769 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_027_-38.0_Oct22_21.19.13.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 83.5 In SetExposureForMean 90, exposure time changed to 2.2400 Trimming A by 20 55, reference by 20 60 Image shift -0.085 -0.170 IS units 378.3 392.6 unbinned pixels; need stage -0.147 0.154 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -18.6 5.3 unbinned pixels; 3.9 5.5 nm along two shift axes; -1.3 -6.7 nm on specimen axes Rate = 33.91808 A/sec -7.8 3.8 unbinned pixels; 2.2 2.1 nm along two shift axes; -1.1 -2.9 nm on specimen axes Rate = 15.25716 A/sec -5.0 1.8 unbinned pixels; 1.2 1.4 nm along two shift axes; -0.5 -1.8 nm on specimen axes Rate = 9.24428 A/sec -3.6 1.0 unbinned pixels; 0.8 1.1 nm along two shift axes; -0.2 -1.3 nm on specimen axes Rate = 6.50777 A/sec Drift is low enough after shot 4 Measured defocus = -4.59 microns changed by -0.16 to target drift = 0.09 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -3.269 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_028_18.0_Oct22_21.20.33.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 116.0 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.338 0.400 IS units -1418.0 540.4 unbinned pixels; need stage -0.142 -0.546 if reset Tilting to 20 Correcting tilt backlash 1.5 -1.7 unbinned pixels; 0.7 0.3 nm along two shift axes; 0.5 0.6 nm on specimen axes Rate = 3.93828 A/sec Drift is low enough after shot 1 Measured defocus = -4.67 microns changed by -0.08 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -3.349 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_029_20.0_Oct22_21.21.31.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 90.0 In SetExposureForMean 90, change by a factor of 1.0007 would require too small a change in exposure time Image shift -0.282 0.253 IS units -964.1 525.6 unbinned pixels; need stage -0.151 -0.381 if reset Tilting to -40 -40.00 degrees 0.3 -3.4 unbinned pixels; 1.2 0.3 nm along two shift axes; 1.2 0.2 nm on specimen axes Rate = 6.09699 A/sec Drift is low enough after shot 1 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.04 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.751 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_030_-40.0_Oct22_21.22.32.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 67.8 In SetExposureForMean 90, exposure time changed to 2.4000 Trimming A by 20 55, reference by 20 55 Image shift -0.058 -0.270 IS units 673.6 413.1 unbinned pixels; need stage -0.163 0.293 if reset Tilting to -42 1.9 -2.0 unbinned pixels; 0.9 0.4 nm along two shift axes; 0.6 0.7 nm on specimen axes Rate = 4.84427 A/sec Drift is low enough after shot 1 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.08 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.705 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_031_-42.0_Oct22_21.23.28.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 88.4 In SetExposureForMean 90, change by a factor of 1.0129 would require too small a change in exposure time Trimming A by 20 50, reference by 20 55 Image shift -0.031 -0.318 IS units 830.2 386.9 unbinned pixels; need stage -0.159 0.378 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -13.0 2.8 unbinned pixels; 2.5 4.0 nm along two shift axes; -0.6 -4.7 nm on specimen axes Rate = 23.40736 A/sec -7.3 2.9 unbinned pixels; 1.8 2.1 nm along two shift axes; -0.8 -2.6 nm on specimen axes Rate = 13.77549 A/sec -6.2 1.7 unbinned pixels; 1.3 1.8 nm along two shift axes; -0.4 -2.2 nm on specimen axes Rate = 11.24531 A/sec -4.9 1.4 unbinned pixels; 1.0 1.4 nm along two shift axes; -0.3 -1.7 nm on specimen axes Rate = 8.86389 A/sec -3.8 0.9 unbinned pixels; 0.7 1.2 nm along two shift axes; -0.2 -1.4 nm on specimen axes Rate = 6.90906 A/sec Drift is low enough after shot 5 Measured defocus = -4.62 microns changed by -0.13 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -3.483 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_032_22.0_Oct22_21.24.54.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 121.0 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.305 0.319 IS units -1165.2 528.0 unbinned pixels; need stage -0.146 -0.463 if reset Tilting to 24 Correcting tilt backlash -4.7 -1.8 unbinned pixels; 0.0 1.8 nm along two shift axes; 0.8 -1.6 nm on specimen axes Rate = 8.84351 A/sec -0.5 4.1 unbinned pixels; 1.4 0.3 nm along two shift axes; -1.4 -0.3 nm on specimen axes Rate = 7.22613 A/sec Drift is low enough after shot 2 Measured defocus = -4.70 microns changed by -0.05 to target drift = 0.05 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -3.544 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_033_24.0_Oct22_21.25.59.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 87.5 In SetExposureForMean 90, change by a factor of 1.0298 would require too small a change in exposure time Image shift -0.255 0.188 IS units -761.4 516.5 unbinned pixels; need stage -0.154 -0.313 if reset Tilting to -44 -44.00 degrees -0.2 -1.0 unbinned pixels; 0.3 0.2 nm along two shift axes; 0.3 -0.0 nm on specimen axes Rate = 1.72152 A/sec Drift is low enough after shot 1 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.05 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.662 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_034_-44.0_Oct22_21.27.01.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 61.8 In SetExposureForMean 90, exposure time changed to 2.5600 Trimming A by 20 40, reference by 20 45 Image shift -0.009 -0.384 IS units 1030.6 390.7 unbinned pixels; need stage -0.166 0.489 if reset Tilting to -46 1.8 -3.7 unbinned pixels; 1.4 0.1 nm along two shift axes; 1.3 0.7 nm on specimen axes Rate = 7.239 A/sec Drift is low enough after shot 1 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.08 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.637 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_035_-46.0_Oct22_21.27.57.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 85.4 In SetExposureForMean 90, exposure time changed to 2.7200 Trimming A by 20 30, reference by 20 40 Image shift 0.018 -0.438 IS units 1206.0 367.3 unbinned pixels; need stage -0.164 0.597 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -14.5 2.6 unbinned pixels; 2.6 4.5 nm along two shift axes; -0.5 -5.2 nm on specimen axes Rate = 25.81556 A/sec -7.3 5.8 unbinned pixels; 2.8 1.7 nm along two shift axes; -1.8 -2.7 nm on specimen axes Rate = 16.29238 A/sec -6.4 2.2 unbinned pixels; 1.5 1.9 nm along two shift axes; -0.6 -2.3 nm on specimen axes Rate = 11.8669 A/sec -4.6 1.7 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.62069 A/sec -3.8 1.3 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 7.07179 A/sec Drift is low enough after shot 5 Measured defocus = -4.59 microns changed by -0.16 to target drift = 0.09 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -3.706 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_036_26.0_Oct22_21.29.25.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 133.0 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.306 0.300 IS units -1113.9 548.4 unbinned pixels; need stage -0.154 -0.458 if reset Tilting to 28 Correcting tilt backlash -4.3 -4.5 unbinned pixels; 1.0 2.0 nm along two shift axes; 1.7 -1.4 nm on specimen axes Rate = 11.0376 A/sec -0.7 6.8 unbinned pixels; 2.3 0.6 nm along two shift axes; -2.4 -0.5 nm on specimen axes Rate = 12.0117 A/sec -0.5 2.7 unbinned pixels; 1.0 0.2 nm along two shift axes; -0.9 -0.3 nm on specimen axes Rate = 4.85936 A/sec Drift is low enough after shot 3 Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.06 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -3.717 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_037_28.0_Oct22_21.30.35.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 92.3 In SetExposureForMean 90, change by a factor of 0.9769 would require too small a change in exposure time Image shift -0.248 0.156 IS units -666.7 525.5 unbinned pixels; need stage -0.160 -0.286 if reset Tilting to -48 -48.00 degrees 1.3 -4.0 unbinned pixels; 1.5 0.0 nm along two shift axes; 1.4 0.6 nm on specimen axes Rate = 7.40725 A/sec Drift is low enough after shot 1 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -0.624 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_038_-48.0_Oct22_21.31.37.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 60.4 In SetExposureForMean 90, exposure time changed to 2.8800 Trimming A by 20 25, reference by 20 30 Image shift 0.046 -0.535 IS units 1494.3 383.8 unbinned pixels; need stage -0.178 0.771 if reset Tilting to -50 -1.0 -5.9 unbinned pixels; 1.8 1.0 nm along two shift axes; 2.1 -0.2 nm on specimen axes Rate = 10.54142 A/sec 3.2 2.9 unbinned pixels; 0.6 1.4 nm along two shift axes; -1.1 1.0 nm on specimen axes Rate = 7.63359 A/sec Drift is low enough after shot 2 Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.04 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.616 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_039_-50.0_Oct22_21.32.39.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 85.0 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift 0.091 -0.612 IS units 1746.0 335.3 unbinned pixels; need stage -0.169 0.944 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -11.6 3.6 unbinned pixels; 2.6 3.4 nm along two shift axes; -0.9 -4.2 nm on specimen axes Rate = 21.34117 A/sec -9.4 2.8 unbinned pixels; 2.0 2.8 nm along two shift axes; -0.7 -3.4 nm on specimen axes Rate = 17.13402 A/sec -7.6 2.8 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.8 -2.8 nm on specimen axes Rate = 14.20473 A/sec -5.5 2.1 unbinned pixels; 1.3 1.6 nm along two shift axes; -0.6 -2.0 nm on specimen axes Rate = 10.27491 A/sec -3.3 1.8 unbinned pixels; 1.0 0.9 nm along two shift axes; -0.5 -1.2 nm on specimen axes Rate = 6.57018 A/sec Drift is low enough after shot 5 Measured defocus = -4.59 microns changed by -0.16 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -3.875 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_040_30.0_Oct22_21.34.05.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 143.0 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.260 0.190 IS units -770.0 528.8 unbinned pixels; need stage -0.158 -0.334 if reset Tilting to 32 Correcting tilt backlash -6.9 -0.8 unbinned pixels; 0.5 2.4 nm along two shift axes; 0.5 -2.4 nm on specimen axes Rate = 12.2644 A/sec -1.4 6.1 unbinned pixels; 2.2 0.2 nm along two shift axes; -2.1 -0.7 nm on specimen axes Rate = 10.97343 A/sec -1.4 2.7 unbinned pixels; 1.1 0.2 nm along two shift axes; -0.9 -0.6 nm on specimen axes Rate = 5.33779 A/sec Drift is low enough after shot 3 Measured defocus = -4.72 microns changed by -0.03 to target drift = 0.07 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -3.913 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_041_32.0_Oct22_21.35.15.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 88.4 In SetExposureForMean 90, change by a factor of 1.0199 would require too small a change in exposure time Image shift -0.214 0.071 IS units -403.8 515.2 unbinned pixels; need stage -0.165 -0.188 if reset Tilting to -52 -52.00 degrees 5.2 -4.9 unbinned pixels; 2.2 1.1 nm along two shift axes; 1.6 2.0 nm on specimen axes Rate = 12.50647 A/sec 3.9 2.4 unbinned pixels; 0.3 1.6 nm along two shift axes; -1.0 1.3 nm on specimen axes Rate = 8.03617 A/sec 2.2 0.8 unbinned pixels; 0.0 0.8 nm along two shift axes; -0.4 0.7 nm on specimen axes Rate = 4.06915 A/sec Drift is low enough after shot 3 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.06 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.568 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_042_-52.0_Oct22_21.36.28.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 52.6 In SetExposureForMean 90, exposure time changed to 3.3600 Image shift 0.129 -0.730 IS units 2105.7 342.7 unbinned pixels; need stage -0.183 1.191 if reset Tilting to -54 -0.7 -3.5 unbinned pixels; 1.1 0.7 nm along two shift axes; 1.2 -0.1 nm on specimen axes Rate = 6.28637 A/sec Drift is low enough after shot 1 Measured defocus = -4.73 microns changed by -0.02 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.574 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_043_-54.0_Oct22_21.37.25.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 85.1 In SetExposureForMean 90, exposure time changed to 3.5200 Image shift 0.161 -0.790 IS units 2299.6 312.9 unbinned pixels; need stage -0.178 1.366 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -17.1 3.4 unbinned pixels; 3.1 5.3 nm along two shift axes; -0.7 -6.1 nm on specimen axes Rate = 30.55629 A/sec -10.5 3.2 unbinned pixels; 2.3 3.1 nm along two shift axes; -0.8 -3.8 nm on specimen axes Rate = 19.21887 A/sec -7.5 2.0 unbinned pixels; 1.5 2.2 nm along two shift axes; -0.5 -2.7 nm on specimen axes Rate = 13.53787 A/sec -5.5 2.1 unbinned pixels; 1.3 1.6 nm along two shift axes; -0.6 -2.0 nm on specimen axes Rate = 10.22339 A/sec -6.7 1.0 unbinned pixels; 1.1 2.1 nm along two shift axes; -0.1 -2.4 nm on specimen axes Rate = 11.79235 A/sec -1.9 2.0 unbinned pixels; 0.9 0.4 nm along two shift axes; -0.6 -0.7 nm on specimen axes Rate = 4.75374 A/sec Drift is low enough after shot 6 Measured defocus = -4.62 microns changed by -0.13 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -4.040 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_044_34.0_Oct22_21.38.58.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 157.8 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.238 0.109 IS units -530.0 544.1 unbinned pixels; need stage -0.171 -0.247 if reset Tilting to 36 Correcting tilt backlash -2.5 -2.0 unbinned pixels; 0.4 1.1 nm along two shift axes; 0.8 -0.8 nm on specimen axes Rate = 5.59847 A/sec Drift is low enough after shot 1 Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.09 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -4.034 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_045_36.0_Oct22_21.39.59.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 90.5 In SetExposureForMean 90, change by a factor of 0.9952 would require too small a change in exposure time Image shift -0.191 0.004 IS units -199.6 517.0 unbinned pixels; need stage -0.172 -0.108 if reset Tilting to -56 -56.00 degrees 2.8 -4.6 unbinned pixels; 1.9 0.4 nm along two shift axes; 1.5 1.1 nm on specimen axes Rate = 9.52347 A/sec 7.9 4.2 unbinned pixels; 0.5 3.1 nm along two shift axes; -1.7 2.7 nm on specimen axes Rate = 15.77503 A/sec 2.0 0.1 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.1 0.7 nm on specimen axes Rate = 3.57507 A/sec Drift is low enough after shot 3 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.04 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.546 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_046_-56.0_Oct22_21.41.17.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 47.7 In SetExposureForMean 90, exposure time changed to 4.0000 Image shift 0.190 -0.877 IS units 2561.6 318.1 unbinned pixels; need stage -0.188 1.601 if reset Tilting to -58 -1.1 -5.2 unbinned pixels; 1.6 1.0 nm along two shift axes; 1.9 -0.2 nm on specimen axes Rate = 9.47878 A/sec 3.0 3.8 unbinned pixels; 0.9 1.4 nm along two shift axes; -1.4 0.9 nm on specimen axes Rate = 8.46213 A/sec 1.3 0.5 unbinned pixels; 0.0 0.5 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.4771 A/sec Drift is low enough after shot 3 Measured defocus = -4.72 microns changed by -0.03 to target drift = 0.03 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.576 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_047_-58.0_Oct22_21.42.25.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 82.4 In SetExposureForMean 90, exposure time changed to 4.4800 Image shift 0.230 -0.954 IS units 2811.7 282.4 unbinned pixels; need stage -0.184 1.859 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -15.7 1.9 unbinned pixels; 2.5 5.0 nm along two shift axes; -0.2 -5.6 nm on specimen axes Rate = 27.73635 A/sec -10.0 2.0 unbinned pixels; 1.8 3.1 nm along two shift axes; -0.4 -3.6 nm on specimen axes Rate = 17.88059 A/sec -7.3 2.5 unbinned pixels; 1.7 2.1 nm along two shift axes; -0.6 -2.6 nm on specimen axes Rate = 13.4853 A/sec -6.3 1.6 unbinned pixels; 1.3 1.9 nm along two shift axes; -0.4 -2.3 nm on specimen axes Rate = 11.38894 A/sec -4.6 1.1 unbinned pixels; 0.9 1.4 nm along two shift axes; -0.2 -1.6 nm on specimen axes Rate = 8.24512 A/sec -3.4 1.3 unbinned pixels; 0.8 1.0 nm along two shift axes; -0.4 -1.2 nm on specimen axes Rate = 6.29494 A/sec Drift is low enough after shot 6 Measured defocus = -4.65 microns changed by -0.10 to target drift = 0.09 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -4.138 um Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_048_38.0_Oct22_21.44.03.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 189.2 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.203 0.026 IS units -270.8 527.8 unbinned pixels; need stage -0.173 -0.143 if reset Tilting to 40 Correcting tilt backlash -4.0 -2.0 unbinned pixels; 0.2 1.6 nm along two shift axes; 0.8 -1.3 nm on specimen axes Rate = 7.83068 A/sec Drift is low enough after shot 1 Measured defocus = -4.72 microns changed by -0.03 to target drift = 0.10 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -4.180 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_049_40.0_Oct22_21.45.04.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 84.9 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.156 -0.064 IS units 20.3 486.1 unbinned pixels; need stage -0.168 -0.011 if reset Tilting to -60 -60.00 degrees 1.4 -5.8 unbinned pixels; 2.1 0.2 nm along two shift axes; 2.0 0.7 nm on specimen axes Rate = 10.60494 A/sec 5.4 2.9 unbinned pixels; 0.3 2.1 nm along two shift axes; -1.2 1.8 nm on specimen axes Rate = 10.8459 A/sec 1.8 0.8 unbinned pixels; 0.0 0.7 nm along two shift axes; -0.3 0.6 nm on specimen axes Rate = 3.54596 A/sec Drift is low enough after shot 3 Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.05 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.561 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_050_-60.0_Oct22_21.46.18.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 40.9 In SetExposureForMean 90, exposure time changed to 4.9600 Image shift 0.270 -1.036 IS units 3074.1 250.3 unbinned pixels; need stage -0.181 2.158 if reset Tilting to -62 -1.5 -4.5 unbinned pixels; 1.3 1.0 nm along two shift axes; 1.6 -0.4 nm on specimen axes Rate = 8.29657 A/sec 2.9 3.4 unbinned pixels; 0.8 1.4 nm along two shift axes; -1.3 0.9 nm on specimen axes Rate = 7.89274 A/sec Drift is low enough after shot 2 Measured defocus = -4.67 microns changed by -0.08 to target drift = 0.04 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.630 um Dewars are NOT busy filling 31 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_051_-62.0_Oct22_21.47.21.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 78.8 In SetExposureForMean 90, exposure time changed to 5.7600 Image shift 0.310 -1.113 IS units 3321.3 214.7 unbinned pixels; need stage -0.176 2.487 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -19.2 -0.1 unbinned pixels; 2.2 6.4 nm along two shift axes; 0.6 -6.7 nm on specimen axes Rate = 33.74868 A/sec -8.7 3.1 unbinned pixels; 2.1 2.5 nm along two shift axes; -0.8 -3.2 nm on specimen axes Rate = 16.26007 A/sec -7.8 1.7 unbinned pixels; 1.5 2.4 nm along two shift axes; -0.4 -2.8 nm on specimen axes Rate = 13.98961 A/sec -6.7 1.7 unbinned pixels; 1.4 2.0 nm along two shift axes; -0.4 -2.4 nm on specimen axes Rate = 12.18309 A/sec -4.0 2.0 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.6 -1.4 nm on specimen axes Rate = 7.77052 A/sec Drift is low enough after shot 5 Measured defocus = -4.62 microns changed by -0.13 to target drift = 0.13 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.10 nm/sec Defocus = -4.308 um Dewars are NOT busy filling 36 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_052_42.0_Oct22_21.48.51.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 226.0 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.154 -0.075 IS units 51.0 489.5 unbinned pixels; need stage -0.170 0.003 if reset Tilting to 44 Correcting tilt backlash -8.4 -6.4 unbinned pixels; 1.1 3.6 nm along two shift axes; 2.5 -2.8 nm on specimen axes Rate = 18.63508 A/sec -0.9 3.9 unbinned pixels; 1.4 0.2 nm along two shift axes; -1.3 -0.4 nm on specimen axes Rate = 6.97224 A/sec Drift is low enough after shot 2 Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.08 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -4.377 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_053_44.0_Oct22_21.49.59.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 84.1 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.114 -0.134 IS units 250.9 439.3 unbinned pixels; need stage -0.159 0.104 if reset Tilting to -64 -64.00 degrees 1.2 -5.6 unbinned pixels; 2.0 0.3 nm along two shift axes; 1.9 0.6 nm on specimen axes Rate = 10.12911 A/sec 5.4 3.6 unbinned pixels; 0.6 2.2 nm along two shift axes; -1.4 1.8 nm on specimen axes Rate = 11.36025 A/sec 1.8 1.6 unbinned pixels; 0.3 0.8 nm along two shift axes; -0.6 0.6 nm on specimen axes Rate = 4.23501 A/sec Drift is low enough after shot 3 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.602 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_054_-64.0_Oct22_21.51.14.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 32.5 In SetExposureForMean 90, exposure time changed to 6.8800 Image shift 0.357 -1.204 IS units 3614.7 173.8 unbinned pixels; need stage -0.171 2.903 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash -15.6 1.4 unbinned pixels; 2.3 5.0 nm along two shift axes; -0.0 -5.5 nm on specimen axes Rate = 27.49268 A/sec -8.4 4.1 unbinned pixels; 2.3 2.3 nm along two shift axes; -1.2 -3.1 nm on specimen axes Rate = 16.37617 A/sec -6.8 2.6 unbinned pixels; 1.7 1.9 nm along two shift axes; -0.7 -2.5 nm on specimen axes Rate = 12.81375 A/sec -5.0 2.6 unbinned pixels; 1.4 1.4 nm along two shift axes; -0.7 -1.8 nm on specimen axes Rate = 9.88255 A/sec -3.8 1.8 unbinned pixels; 1.0 1.0 nm along two shift axes; -0.5 -1.4 nm on specimen axes Rate = 7.2985 A/sec Drift is low enough after shot 5 Measured defocus = -4.57 microns changed by -0.18 to target drift = 0.09 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -4.554 um Dewars are NOT busy filling 43 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_055_46.0_Oct22_21.52.42.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 246.6 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.136 -0.138 IS units 240.0 500.7 unbinned pixels; need stage -0.180 0.099 if reset Tilting to 48 Correcting tilt backlash -3.5 -2.8 unbinned pixels; 0.5 1.5 nm along two shift axes; 1.1 -1.1 nm on specimen axes Rate = 7.94497 A/sec Drift is low enough after shot 1 Measured defocus = -4.72 microns changed by -0.03 to target drift = 0.07 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -4.592 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_02_4_056_48.0_Oct22_21.53.45.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 87.3 In SetExposureForMean 90, change by a factor of 1.0254 would require too small a change in exposure time Image shift -0.085 -0.222 IS units 518.1 442.7 unbinned pixels; need stage -0.168 0.252 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -3.04 microns changed by -1.71 to target drift = 0.37 nm/sec Focus area changed from stored state, axis position -2.40 angle 0, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_03.st Running script 1 at item 6 (110.34, -136.95) 21:54:38 Refine ZLP: additional offset = -0.0 eV; total offset = 5.7 eV Item 6: Stage: 110.34 -136.95 -235 Label: 6 Note: Sec 4 - anchor_maps.map Target defocus is: -4.75 um Target defocus is: -4.00 um Target defocus = -4 Trimming A by 20 25, reference by 20 20 Angle = -4.40, increment = 0.60, shift = -14.6 -6.0 pixels, -0.035 um Y Trimming A by 20 25, reference by 20 20 Angle = -3.20, increment = 1.80, shift = -38.1 -11.5 pixels, -0.089 um Y Trimming A by 30 35, reference by 20 20 Angle = 0.41, increment = 5.41, shift = -130.3 -27.0 pixels, -0.303 um Y Trimming A by 40 40, reference by 20 20 Angle = 3.00, increment = 8.00, shift = -183.4 -36.5 pixels, -0.425 um Y Rough eucentricity: changing Z by 3.05 to -231.89, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 23 75, reference by 21 75 Angle = 18.00, shift = -16.5, 7.6 pixels, 0.022, -0.036 um, IS = -0.022, 0.036 Trimming A by 45 50, reference by 23 75 Angle = 11.00, shift = -49.6, -5.3 pixels, 0.001, -0.114 um, IS = -0.022, 0.149 Trimming A by 35 35, reference by 45 45 Angle = 4.00, shift = -42.7, -2.2 pixels, 0.006, -0.097 um, IS = -0.028, 0.247 Trimming A by 80 20 55 20, reference by 80 20 50 25 Angle = -4.00, shift = -23.7, -20.6 pixels, -0.041, -0.059 um, IS = 0.013, 0.306 Trimming A by 20 30, reference by 25 25 Angle = -11.00, shift = -65.7, -11.7 pixels, -0.010, -0.152 um, IS = 0.022, 0.458 Trimming A by 25 30, reference by 20 30 Angle = -18.00, shift = -21.6, 1.4 pixels, 0.009, -0.049 um, IS = 0.014, 0.506 Trimming A by 20 30, reference by 20 30 Angle = -24.00, shift = 31.4, 10.5 pixels, 0.016, 0.074 um, IS = -0.002, 0.432 General solution delZ 0.64, delY 0.54, yZero 0.30 solution with assumed YZero 0.28: delZ 0.64, delY 0.21 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.64 microns and lateral displacement of -0.54 microns Trimming A by 40 20 25 25, reference by 20 20 20 40 Disparity in stage position after aligning to center of map frame: -0.08 -0.66 Aligning to target with image shift equivalent to stage shift: -0.08 -0.67 Disparity in stage position after aligning to map item itself: -0.08 -0.62 (align shift 0.00 0.02) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 110.36 -136.92 -232.51 -0.4 1.7 unbinned pixels; 0.6 0.1 nm along two shift axes; -0.6 -0.2 nm on specimen axes Rate = 3.01652 A/sec Drift is low enough after shot 1 Measured defocus = -3.50 microns changed by -0.50 to target drift = 0.04 nm/sec Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.522 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_000_-10.0_Oct22_21.58.27.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 89.3 In SetExposureForMean 90, change by a factor of 1.0084 would require too small a change in exposure time Image shift -0.341 0.530 IS units -1773.1 423.7 unbinned pixels; need stage -0.091 -0.651 if reset Tilting to -8 Correcting tilt backlash 9.1 -0.4 unbinned pixels; 1.2 3.0 nm along two shift axes; -0.1 3.2 nm on specimen axes Rate = 15.94032 A/sec 4.3 0.6 unbinned pixels; 0.3 1.5 nm along two shift axes; -0.3 1.5 nm on specimen axes Rate = 7.66922 A/sec Drift is low enough after shot 2 Measured defocus = -3.89 microns changed by -0.11 to target drift = 0.07 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -0.633 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_001_-8.0_Oct22_21.59.29.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 89.8 In SetExposureForMean 90, change by a factor of 1.0027 would require too small a change in exposure time Image shift -0.378 0.532 IS units -1812.5 523.8 unbinned pixels; need stage -0.124 -0.664 if reset Tilting to -12 -12.00 degrees -3.0 2.1 unbinned pixels; 1.0 0.7 nm along two shift axes; -0.6 -1.1 nm on specimen axes Rate = 6.41983 A/sec Drift is low enough after shot 1 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.08 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.449 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_002_-12.0_Oct22_22.00.25.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 89.0 In SetExposureForMean 90, change by a factor of 1.0114 would require too small a change in exposure time Image shift -0.432 0.633 IS units -2141.0 573.5 unbinned pixels; need stage -0.131 -0.793 if reset Tilting to -14 0.3 1.8 unbinned pixels; 0.6 0.3 nm along two shift axes; -0.6 0.0 nm on specimen axes Rate = 3.16966 A/sec Drift is low enough after shot 1 Measured defocus = -4.12 microns changed by 0.12 to target drift = 0.01 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.325 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_003_-14.0_Oct22_22.01.20.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 88.3 In SetExposureForMean 90, change by a factor of 1.0199 would require too small a change in exposure time Image shift -0.482 0.734 IS units -2462.3 614.2 unbinned pixels; need stage -0.136 -0.918 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 9.8 -9.0 unbinned pixels; 4.1 2.2 nm along two shift axes; 2.9 3.7 nm on specimen axes Rate = 23.34274 A/sec 6.3 0.6 unbinned pixels; 0.5 2.1 nm along two shift axes; -0.4 2.2 nm on specimen axes Rate = 11.04608 A/sec 3.3 -0.2 unbinned pixels; 0.4 1.1 nm along two shift axes; -0.0 1.2 nm on specimen axes Rate = 5.80121 A/sec Drift is low enough after shot 3 Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.07 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.721 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_004_-6.0_Oct22_22.02.31.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 89.9 In SetExposureForMean 90, change by a factor of 1.0009 would require too small a change in exposure time Image shift -0.311 0.390 IS units -1363.0 474.4 unbinned pixels; need stage -0.121 -0.500 if reset Tilting to -4 Correcting tilt backlash -5.6 -4.9 unbinned pixels; 1.0 2.4 nm along two shift axes; 1.9 -1.8 nm on specimen axes Rate = 13.06553 A/sec -0.9 1.8 unbinned pixels; 0.7 0.1 nm along two shift axes; -0.6 -0.4 nm on specimen axes Rate = 3.54115 A/sec Drift is low enough after shot 2 Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -0.845 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_005_-4.0_Oct22_22.03.34.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 90.1 In SetExposureForMean 90, change by a factor of 0.9985 would require too small a change in exposure time Image shift -0.276 0.337 IS units -1185.2 429.9 unbinned pixels; need stage -0.111 -0.434 if reset Tilting to -16 -16.00 degrees -4.3 -11.0 unbinned pixels; 3.2 2.7 nm along two shift axes; 4.0 -1.2 nm on specimen axes Rate = 20.85877 A/sec -1.1 2.4 unbinned pixels; 0.9 0.1 nm along two shift axes; -0.8 -0.4 nm on specimen axes Rate = 4.68394 A/sec Drift is low enough after shot 2 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -0.258 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_006_-16.0_Oct22_22.04.37.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 87.6 In SetExposureForMean 90, change by a factor of 1.0274 would require too small a change in exposure time Image shift -0.411 0.559 IS units -1919.1 586.1 unbinned pixels; need stage -0.143 -0.726 if reset Tilting to -18 3.6 -0.2 unbinned pixels; 0.5 1.2 nm along two shift axes; -0.1 1.3 nm on specimen axes Rate = 6.40442 A/sec Drift is low enough after shot 1 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.176 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_007_-18.0_Oct22_22.05.33.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 86.9 In SetExposureForMean 90, change by a factor of 1.0363 would require too small a change in exposure time Image shift -0.412 0.572 IS units -1955.5 576.9 unbinned pixels; need stage -0.138 -0.747 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 8.1 -1.0 unbinned pixels; 1.3 2.6 nm along two shift axes; 0.1 2.9 nm on specimen axes Rate = 14.36413 A/sec 6.4 1.2 unbinned pixels; 0.3 2.3 nm along two shift axes; -0.6 2.2 nm on specimen axes Rate = 11.46082 A/sec 2.6 0.2 unbinned pixels; 0.2 0.9 nm along two shift axes; -0.2 0.9 nm on specimen axes Rate = 4.62844 A/sec Drift is low enough after shot 3 Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.05 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.930 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_008_-2.0_Oct22_22.06.43.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 89.9 In SetExposureForMean 90, change by a factor of 1.0015 would require too small a change in exposure time Image shift -0.302 0.356 IS units -1261.4 484.0 unbinned pixels; need stage -0.128 -0.462 if reset Tilting to 0 Correcting tilt backlash -4.2 0.1 unbinned pixels; 0.5 1.4 nm along two shift axes; 0.1 -1.5 nm on specimen axes Rate = 7.3404 A/sec Drift is low enough after shot 1 Measured defocus = -3.89 microns changed by -0.11 to target drift = 0.08 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -1.038 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_009_0.0_Oct22_22.07.41.tif Saved Z = 9, 0.00 degrees Mean of buffer A = 89.8 In SetExposureForMean 90, change by a factor of 1.0019 would require too small a change in exposure time Image shift -0.303 0.337 IS units -1210.4 505.2 unbinned pixels; need stage -0.137 -0.444 if reset Tilting to -20 -20.00 degrees -3.8 -2.6 unbinned pixels; 0.4 1.6 nm along two shift axes; 1.0 -1.3 nm on specimen axes Rate = 8.1191 A/sec -0.3 0.2 unbinned pixels; 0.1 0.1 nm along two shift axes; -0.1 -0.1 nm on specimen axes Rate = 0.70707 A/sec Drift is low enough after shot 2 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.03 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.129 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_010_-20.0_Oct22_22.08.45.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 86.1 In SetExposureForMean 90, change by a factor of 1.0454 would require too small a change in exposure time Image shift -0.392 0.485 IS units -1700.9 605.3 unbinned pixels; need stage -0.156 -0.661 if reset Tilting to -22 1.9 -1.8 unbinned pixels; 0.8 0.4 nm along two shift axes; 0.6 0.7 nm on specimen axes Rate = 4.55906 A/sec Drift is low enough after shot 1 Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.06 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -0.037 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_011_-22.0_Oct22_22.09.40.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 85.0 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.374 0.458 IS units -1608.1 582.8 unbinned pixels; need stage -0.151 -0.634 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 7.5 1.1 unbinned pixels; 0.5 2.6 nm along two shift axes; -0.6 2.6 nm on specimen axes Rate = 13.23993 A/sec 6.8 0.6 unbinned pixels; 0.6 2.3 nm along two shift axes; -0.4 2.4 nm on specimen axes Rate = 12.04638 A/sec 2.8 0.3 unbinned pixels; 0.2 1.0 nm along two shift axes; -0.2 1.0 nm on specimen axes Rate = 4.9803 A/sec Drift is low enough after shot 3 Measured defocus = -3.89 microns changed by -0.11 to target drift = 0.06 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.149 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_012_2.0_Oct22_22.10.51.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 98.8 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.321 0.358 IS units -1285.0 533.7 unbinned pixels; need stage -0.144 -0.472 if reset Tilting to 4 Correcting tilt backlash -15.4 3.4 unbinned pixels; 2.9 4.7 nm along two shift axes; -0.7 -5.5 nm on specimen axes Rate = 27.71816 A/sec -8.6 3.1 unbinned pixels; 2.0 2.5 nm along two shift axes; -0.8 -3.1 nm on specimen axes Rate = 16.02803 A/sec -7.8 2.3 unbinned pixels; 1.7 2.3 nm along two shift axes; -0.6 -2.8 nm on specimen axes Rate = 14.26862 A/sec -5.9 1.5 unbinned pixels; 1.2 1.8 nm along two shift axes; -0.3 -2.1 nm on specimen axes Rate = 10.72969 A/sec -4.6 1.1 unbinned pixels; 0.9 1.4 nm along two shift axes; -0.2 -1.7 nm on specimen axes Rate = 8.30603 A/sec -3.7 1.5 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.4 -1.4 nm on specimen axes Rate = 7.02994 A/sec Drift is low enough after shot 6 Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.13 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -1.254 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_013_4.0_Oct22_22.12.16.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 89.4 In SetExposureForMean 90, change by a factor of 1.0070 would require too small a change in exposure time Image shift -0.351 0.417 IS units -1477.0 559.3 unbinned pixels; need stage -0.147 -0.542 if reset Tilting to -24 -24.00 degrees -1.6 -5.4 unbinned pixels; 1.6 1.2 nm along two shift axes; 1.9 -0.4 nm on specimen axes Rate = 9.97247 A/sec 1.6 2.8 unbinned pixels; 0.8 0.9 nm along two shift axes; -1.0 0.5 nm on specimen axes Rate = 5.74512 A/sec Drift is low enough after shot 2 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.02 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = 0.005 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_014_-24.0_Oct22_22.13.20.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 83.9 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.344 0.383 IS units -1376.3 573.6 unbinned pixels; need stage -0.155 -0.553 if reset Tilting to -26 3.1 -4.9 unbinned pixels; 2.0 0.4 nm along two shift axes; 1.6 1.2 nm on specimen axes Rate = 10.14725 A/sec 3.0 1.9 unbinned pixels; 0.3 1.2 nm along two shift axes; -0.8 1.0 nm on specimen axes Rate = 6.32066 A/sec Drift is low enough after shot 2 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = 0.083 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_015_-26.0_Oct22_22.14.22.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 91.1 In SetExposureForMean 90, change by a factor of 0.9877 would require too small a change in exposure time Image shift -0.318 0.331 IS units -1208.3 550.2 unbinned pixels; need stage -0.152 -0.495 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 3.2 0.5 unbinned pixels; 0.2 1.1 nm along two shift axes; -0.3 1.1 nm on specimen axes Rate = 5.66709 A/sec Drift is low enough after shot 1 Measured defocus = -3.87 microns changed by -0.13 to target drift = 0.06 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.387 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_016_6.0_Oct22_22.15.23.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 98.2 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.338 0.394 IS units -1400.1 545.1 unbinned pixels; need stage -0.144 -0.516 if reset Tilting to 8 Correcting tilt backlash -22.1 6.1 unbinned pixels; 4.6 6.6 nm along two shift axes; -1.5 -7.9 nm on specimen axes Rate = 40.16273 A/sec -11.5 4.8 unbinned pixels; 2.9 3.2 nm along two shift axes; -1.3 -4.2 nm on specimen axes Rate = 21.8118 A/sec -10.0 2.8 unbinned pixels; 2.1 3.0 nm along two shift axes; -0.7 -3.6 nm on specimen axes Rate = 18.20835 A/sec -7.6 2.3 unbinned pixels; 1.7 2.2 nm along two shift axes; -0.6 -2.7 nm on specimen axes Rate = 13.9786 A/sec -5.9 0.9 unbinned pixels; 1.0 1.9 nm along two shift axes; -0.1 -2.1 nm on specimen axes Rate = 10.54823 A/sec -5.8 2.9 unbinned pixels; 1.6 1.6 nm along two shift axes; -0.8 -2.1 nm on specimen axes Rate = 11.40112 A/sec -4.0 1.8 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.5 -1.5 nm on specimen axes Rate = 7.73339 A/sec Drift is low enough after shot 7 Measured defocus = -3.85 microns changed by -0.15 to target drift = 0.13 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -1.538 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_017_8.0_Oct22_22.16.54.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 88.6 In SetExposureForMean 90, change by a factor of 1.0160 would require too small a change in exposure time Image shift -0.373 0.452 IS units -1590.9 584.6 unbinned pixels; need stage -0.152 -0.587 if reset Tilting to -28 -28.00 degrees 0.1 -5.1 unbinned pixels; 1.7 0.6 nm along two shift axes; 1.8 0.2 nm on specimen axes Rate = 8.99033 A/sec 4.0 2.7 unbinned pixels; 0.4 1.7 nm along two shift axes; -1.1 1.3 nm on specimen axes Rate = 8.61371 A/sec 0.9 1.1 unbinned pixels; 0.3 0.4 nm along two shift axes; -0.4 0.3 nm on specimen axes Rate = 2.44062 A/sec Drift is low enough after shot 3 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.03 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = 0.144 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_018_-28.0_Oct22_22.18.04.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 81.5 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.292 0.255 IS units -977.6 552.9 unbinned pixels; need stage -0.160 -0.412 if reset Tilting to -30 1.3 -5.4 unbinned pixels; 1.9 0.2 nm along two shift axes; 1.8 0.6 nm on specimen axes Rate = 9.76819 A/sec 4.1 1.9 unbinned pixels; 0.2 1.6 nm along two shift axes; -0.8 1.4 nm on specimen axes Rate = 7.90751 A/sec Drift is low enough after shot 2 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = 0.206 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_019_-30.0_Oct22_22.19.05.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 88.1 In SetExposureForMean 90, change by a factor of 1.0227 would require too small a change in exposure time Image shift -0.260 0.188 IS units -764.9 529.7 unbinned pixels; need stage -0.159 -0.332 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees 1.0 -1.8 unbinned pixels; 0.7 0.1 nm along two shift axes; 0.6 0.4 nm on specimen axes Rate = 3.5378 A/sec Drift is low enough after shot 1 Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.660 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_020_10.0_Oct22_22.20.06.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 96.7 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.354 0.404 IS units -1442.6 579.0 unbinned pixels; need stage -0.155 -0.537 if reset Tilting to 12 Correcting tilt backlash -16.0 4.6 unbinned pixels; 3.4 4.7 nm along two shift axes; -1.1 -5.7 nm on specimen axes Rate = 29.13771 A/sec -12.0 3.7 unbinned pixels; 2.6 3.5 nm along two shift axes; -0.9 -4.3 nm on specimen axes Rate = 21.99298 A/sec -7.7 2.5 unbinned pixels; 1.7 2.2 nm along two shift axes; -0.7 -2.8 nm on specimen axes Rate = 14.18342 A/sec -7.7 1.2 unbinned pixels; 1.3 2.4 nm along two shift axes; -0.2 -2.7 nm on specimen axes Rate = 13.61662 A/sec -3.4 2.2 unbinned pixels; 1.1 0.8 nm along two shift axes; -0.7 -1.3 nm on specimen axes Rate = 7.09094 A/sec Drift is low enough after shot 5 Measured defocus = -3.87 microns changed by -0.13 to target drift = 0.10 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -1.795 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_021_12.0_Oct22_22.21.26.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 87.4 In SetExposureForMean 90, change by a factor of 1.0302 would require too small a change in exposure time Image shift -0.368 0.433 IS units -1536.4 590.8 unbinned pixels; need stage -0.156 -0.575 if reset Tilting to -32 -32.00 degrees 0.0 -5.2 unbinned pixels; 1.7 0.6 nm along two shift axes; 1.8 0.2 nm on specimen axes Rate = 9.17225 A/sec 4.4 3.7 unbinned pixels; 0.7 1.9 nm along two shift axes; -1.4 1.4 nm on specimen axes Rate = 10.16587 A/sec 1.1 1.3 unbinned pixels; 0.3 0.5 nm along two shift axes; -0.5 0.3 nm on specimen axes Rate = 2.98721 A/sec Drift is low enough after shot 3 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.02 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = 0.273 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_022_-32.0_Oct22_22.22.37.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 78.5 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.238 0.110 IS units -532.7 543.6 unbinned pixels; need stage -0.171 -0.243 if reset Tilting to -34 4.6 -7.1 unbinned pixels; 2.9 0.7 nm along two shift axes; 2.3 1.8 nm on specimen axes Rate = 14.8504 A/sec 3.0 1.8 unbinned pixels; 0.2 1.2 nm along two shift axes; -0.7 1.0 nm on specimen axes Rate = 6.13011 A/sec Drift is low enough after shot 2 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.07 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = 0.311 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_023_-34.0_Oct22_22.23.38.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 84.4 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.186 0.007 IS units -202.0 500.6 unbinned pixels; need stage -0.166 -0.106 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -11.9 1.7 unbinned pixels; 1.9 3.7 nm along two shift axes; -0.2 -4.2 nm on specimen axes Rate = 20.99162 A/sec -5.2 3.1 unbinned pixels; 1.7 1.3 nm along two shift axes; -0.9 -1.9 nm on specimen axes Rate = 10.63527 A/sec -3.3 1.9 unbinned pixels; 1.0 0.9 nm along two shift axes; -0.6 -1.2 nm on specimen axes Rate = 6.73874 A/sec Drift is low enough after shot 3 Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.07 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.933 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_024_14.0_Oct22_22.24.51.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 103.5 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.356 0.402 IS units -1438.9 588.1 unbinned pixels; need stage -0.158 -0.544 if reset Tilting to 16 Correcting tilt backlash -2.1 -1.4 unbinned pixels; 0.2 0.9 nm along two shift axes; 0.6 -0.7 nm on specimen axes Rate = 4.41892 A/sec Drift is low enough after shot 1 Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.10 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.013 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_025_16.0_Oct22_22.25.49.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 85.3 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.336 0.374 IS units -1344.2 558.3 unbinned pixels; need stage -0.151 -0.513 if reset Tilting to -36 -36.00 degrees -1.0 -2.5 unbinned pixels; 0.7 0.6 nm along two shift axes; 0.9 -0.3 nm on specimen axes Rate = 4.71579 A/sec Drift is low enough after shot 1 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.05 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = 0.394 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_026_-36.0_Oct22_22.26.50.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 82.5 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.169 -0.055 IS units -17.7 511.0 unbinned pixels; need stage -0.175 -0.028 if reset Tilting to -38 3.7 -7.1 unbinned pixels; 2.8 0.4 nm along two shift axes; 2.4 1.5 nm on specimen axes Rate = 14.02718 A/sec 3.5 1.5 unbinned pixels; 0.1 1.3 nm along two shift axes; -0.6 1.2 nm on specimen axes Rate = 6.75278 A/sec Drift is low enough after shot 2 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.05 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = 0.440 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_027_-38.0_Oct22_22.27.51.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 87.5 In SetExposureForMean 90, change by a factor of 1.0341 would require too small a change in exposure time Image shift -0.119 -0.154 IS units 299.4 469.1 unbinned pixels; need stage -0.171 0.115 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -21.9 6.0 unbinned pixels; 4.5 6.5 nm along two shift axes; -1.4 -7.9 nm on specimen axes Rate = 39.78801 A/sec -7.7 5.0 unbinned pixels; 2.5 2.0 nm along two shift axes; -1.5 -2.8 nm on specimen axes Rate = 16.03111 A/sec -5.7 1.5 unbinned pixels; 1.2 1.7 nm along two shift axes; -0.3 -2.1 nm on specimen axes Rate = 10.38048 A/sec -3.3 1.2 unbinned pixels; 0.8 0.9 nm along two shift axes; -0.3 -1.2 nm on specimen axes Rate = 6.11829 A/sec Drift is low enough after shot 4 Measured defocus = -3.86 microns changed by -0.14 to target drift = 0.10 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -2.154 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_028_18.0_Oct22_22.29.10.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 101.0 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.382 0.435 IS units -1554.6 627.5 unbinned pixels; need stage -0.168 -0.599 if reset Tilting to 20 Correcting tilt backlash 8.6 -2.9 unbinned pixels; 2.0 2.5 nm along two shift axes; 0.8 3.1 nm on specimen axes Rate = 15.83841 A/sec 2.4 4.2 unbinned pixels; 1.1 1.3 nm along two shift axes; -1.5 0.7 nm on specimen axes Rate = 8.54924 A/sec -0.1 2.0 unbinned pixels; 0.7 0.2 nm along two shift axes; -0.7 -0.1 nm on specimen axes Rate = 3.61479 A/sec Drift is low enough after shot 3 Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.07 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -2.191 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_029_20.0_Oct22_22.30.19.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 91.5 In SetExposureForMean 90, change by a factor of 0.9848 would require too small a change in exposure time Image shift -0.333 0.324 IS units -1206.2 598.9 unbinned pixels; need stage -0.169 -0.475 if reset Tilting to -40 -40.00 degrees 1.7 -4.9 unbinned pixels; 1.8 0.0 nm along two shift axes; 1.7 0.7 nm on specimen axes Rate = 9.07245 A/sec 2.2 2.3 unbinned pixels; 0.5 1.0 nm along two shift axes; -0.9 0.7 nm on specimen axes Rate = 5.68002 A/sec Drift is low enough after shot 2 Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.03 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = 0.468 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_030_-40.0_Oct22_22.31.26.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 77.9 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.082 -0.273 IS units 659.2 484.4 unbinned pixels; need stage -0.187 0.284 if reset Tilting to -42 4.2 -7.3 unbinned pixels; 2.9 0.5 nm along two shift axes; 2.4 1.7 nm on specimen axes Rate = 14.91364 A/sec 2.8 2.0 unbinned pixels; 0.3 1.2 nm along two shift axes; -0.8 0.9 nm on specimen axes Rate = 6.07735 A/sec Drift is low enough after shot 2 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.06 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = 0.509 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_031_-42.0_Oct22_22.32.27.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 89.3 In SetExposureForMean 90, change by a factor of 1.0168 would require too small a change in exposure time Image shift -0.030 -0.373 IS units 981.4 436.1 unbinned pixels; need stage -0.180 0.448 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -13.1 4.6 unbinned pixels; 3.1 3.8 nm along two shift axes; -1.2 -4.7 nm on specimen axes Rate = 24.29377 A/sec -6.1 2.5 unbinned pixels; 1.6 1.7 nm along two shift axes; -0.7 -2.2 nm on specimen axes Rate = 11.62231 A/sec -6.1 1.9 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.2 nm on specimen axes Rate = 11.24172 A/sec -4.5 1.3 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.3 -1.6 nm on specimen axes Rate = 8.26901 A/sec -4.2 1.3 unbinned pixels; 0.9 1.2 nm along two shift axes; -0.3 -1.5 nm on specimen axes Rate = 7.71233 A/sec Drift is low enough after shot 5 Measured defocus = -3.86 microns changed by -0.14 to target drift = 0.14 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.332 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_032_22.0_Oct22_22.33.52.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 106.4 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.363 0.383 IS units -1396.8 624.2 unbinned pixels; need stage -0.172 -0.554 if reset Tilting to 24 Correcting tilt backlash -7.3 -7.1 unbinned pixels; 1.5 3.3 nm along two shift axes; 2.7 -2.3 nm on specimen axes Rate = 17.92977 A/sec -4.3 6.9 unbinned pixels; 2.8 0.6 nm along two shift axes; -2.3 -1.7 nm on specimen axes Rate = 14.23094 A/sec -2.0 2.7 unbinned pixels; 1.1 0.3 nm along two shift axes; -0.9 -0.8 nm on specimen axes Rate = 5.88803 A/sec Drift is low enough after shot 3 Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.360 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_033_24.0_Oct22_22.35.02.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 88.7 In SetExposureForMean 90, change by a factor of 1.0168 would require too small a change in exposure time Image shift -0.293 0.230 IS units -912.9 578.7 unbinned pixels; need stage -0.171 -0.374 if reset Tilting to -44 -44.00 degrees 0.9 -5.1 unbinned pixels; 1.8 0.3 nm along two shift axes; 1.8 0.5 nm on specimen axes Rate = 9.1613 A/sec 3.0 2.9 unbinned pixels; 0.6 1.3 nm along two shift axes; -1.1 1.0 nm on specimen axes Rate = 7.40669 A/sec Drift is low enough after shot 2 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.04 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = 0.587 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_034_-44.0_Oct22_22.36.13.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 73.0 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift 0.014 -0.497 IS units 1361.2 435.0 unbinned pixels; need stage -0.192 0.649 if reset Tilting to -46 1.2 -6.7 unbinned pixels; 2.4 0.4 nm along two shift axes; 2.3 0.6 nm on specimen axes Rate = 12.01774 A/sec 2.1 2.1 unbinned pixels; 0.4 1.0 nm along two shift axes; -0.8 0.7 nm on specimen axes Rate = 5.28389 A/sec Drift is low enough after shot 2 Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = 0.574 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_035_-46.0_Oct22_22.37.14.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 89.7 In SetExposureForMean 90, change by a factor of 1.0165 would require too small a change in exposure time Image shift 0.071 -0.613 IS units 1729.0 390.7 unbinned pixels; need stage -0.188 0.862 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -11.8 5.8 unbinned pixels; 3.3 3.2 nm along two shift axes; -1.7 -4.3 nm on specimen axes Rate = 23.05592 A/sec -5.2 4.7 unbinned pixels; 2.2 1.2 nm along two shift axes; -1.5 -2.0 nm on specimen axes Rate = 12.37438 A/sec -4.6 2.1 unbinned pixels; 1.3 1.3 nm along two shift axes; -0.6 -1.7 nm on specimen axes Rate = 8.96831 A/sec -4.0 1.8 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.5 -1.5 nm on specimen axes Rate = 7.67395 A/sec Drift is low enough after shot 4 Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.10 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.489 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_036_26.0_Oct22_22.38.34.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 110.6 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.338 0.294 IS units -1130.8 641.3 unbinned pixels; need stage -0.186 -0.468 if reset Tilting to 28 Correcting tilt backlash -6.1 -3.4 unbinned pixels; 0.4 2.4 nm along two shift axes; 1.4 -2.0 nm on specimen axes Rate = 12.2697 A/sec -3.2 7.2 unbinned pixels; 2.8 0.2 nm along two shift axes; -2.4 -1.3 nm on specimen axes Rate = 13.78972 A/sec -1.6 3.2 unbinned pixels; 1.3 0.2 nm along two shift axes; -1.1 -0.7 nm on specimen axes Rate = 6.35601 A/sec Drift is low enough after shot 3 Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.509 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_037_28.0_Oct22_22.39.43.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 85.2 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.275 0.155 IS units -691.3 602.0 unbinned pixels; need stage -0.186 -0.299 if reset Tilting to -48 -48.00 degrees 2.4 -0.1 unbinned pixels; 0.3 0.8 nm along two shift axes; -0.0 0.9 nm on specimen axes Rate = 4.25499 A/sec Drift is low enough after shot 1 Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.09 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = 0.674 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_038_-48.0_Oct22_22.40.45.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 73.7 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift 0.081 -0.662 IS units 1873.0 409.4 unbinned pixels; need stage -0.198 0.970 if reset Tilting to -50 0.0 -3.7 unbinned pixels; 1.2 0.4 nm along two shift axes; 1.3 0.1 nm on specimen axes Rate = 6.45308 A/sec Drift is low enough after shot 1 Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.06 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = 0.672 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_039_-50.0_Oct22_22.41.41.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 87.9 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift 0.104 -0.703 IS units 2007.0 384.5 unbinned pixels; need stage -0.194 1.085 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -7.7 5.4 unbinned pixels; 2.7 1.9 nm along two shift axes; -1.7 -2.9 nm on specimen axes Rate = 16.50901 A/sec -2.6 3.1 unbinned pixels; 1.3 0.5 nm along two shift axes; -1.0 -1.0 nm on specimen axes Rate = 7.07245 A/sec Drift is low enough after shot 2 Measured defocus = -3.90 microns changed by -0.10 to target drift = 0.12 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.13 nm/sec Defocus = -2.611 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_040_30.0_Oct22_22.42.50.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 121.0 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.304 0.201 IS units -842.7 635.7 unbinned pixels; need stage -0.193 -0.368 if reset Tilting to 32 Correcting tilt backlash -6.4 -6.9 unbinned pixels; 1.5 2.9 nm along two shift axes; 2.6 -2.0 nm on specimen axes Rate = 16.55572 A/sec -3.5 5.9 unbinned pixels; 2.4 0.4 nm along two shift axes; -2.0 -1.4 nm on specimen axes Rate = 12.00896 A/sec -2.6 2.5 unbinned pixels; 1.1 0.5 nm along two shift axes; -0.8 -1.0 nm on specimen axes Rate = 6.27198 A/sec Drift is low enough after shot 3 Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -2.635 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_041_32.0_Oct22_22.44.04.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 88.6 In SetExposureForMean 90, change by a factor of 1.0187 would require too small a change in exposure time Image shift -0.223 0.118 IS units -540.2 494.2 unbinned pixels; need stage -0.153 -0.244 if reset Tilting to -52 -52.00 degrees 3.8 -5.9 unbinned pixels; 2.4 0.6 nm along two shift axes; 2.0 1.5 nm on specimen axes Rate = 12.36243 A/sec 3.2 1.8 unbinned pixels; 0.2 1.3 nm along two shift axes; -0.7 1.1 nm on specimen axes Rate = 6.58397 A/sec Drift is low enough after shot 2 Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = 0.680 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_042_-52.0_Oct22_22.45.12.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 66.6 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift 0.131 -0.803 IS units 2303.3 407.7 unbinned pixels; need stage -0.211 1.301 if reset Tilting to -54 0.1 -4.0 unbinned pixels; 1.3 0.4 nm along two shift axes; 1.4 0.2 nm on specimen axes Rate = 7.06063 A/sec Drift is low enough after shot 1 Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.05 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = 0.680 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_043_-54.0_Oct22_22.46.07.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 89.3 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift 0.156 -0.846 IS units 2444.3 380.8 unbinned pixels; need stage -0.206 1.449 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -11.2 7.3 unbinned pixels; 3.7 2.8 nm along two shift axes; -2.2 -4.1 nm on specimen axes Rate = 23.44676 A/sec -8.3 5.4 unbinned pixels; 2.7 2.1 nm along two shift axes; -1.6 -3.1 nm on specimen axes Rate = 17.32482 A/sec -6.0 2.3 unbinned pixels; 1.5 1.7 nm along two shift axes; -0.6 -2.2 nm on specimen axes Rate = 11.22392 A/sec -4.7 1.4 unbinned pixels; 1.0 1.4 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.56529 A/sec -3.9 1.3 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 7.14355 A/sec Drift is low enough after shot 5 Measured defocus = -3.87 microns changed by -0.13 to target drift = 0.11 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.774 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_044_34.0_Oct22_22.47.40.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 128.9 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.267 0.113 IS units -568.3 620.7 unbinned pixels; need stage -0.196 -0.267 if reset Tilting to 36 Correcting tilt backlash -9.8 -3.4 unbinned pixels; 0.0 3.7 nm along two shift axes; 1.5 -3.3 nm on specimen axes Rate = 18.31288 A/sec -3.0 5.2 unbinned pixels; 2.1 0.4 nm along two shift axes; -1.7 -1.2 nm on specimen axes Rate = 10.50966 A/sec -3.0 2.0 unbinned pixels; 1.0 0.7 nm along two shift axes; -0.6 -1.1 nm on specimen axes Rate = 6.30285 A/sec Drift is low enough after shot 3 Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.09 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.809 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_045_36.0_Oct22_22.48.55.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 90.0 In SetExposureForMean 90, change by a factor of 1.0045 would require too small a change in exposure time Image shift -0.213 0.047 IS units -337.4 535.6 unbinned pixels; need stage -0.174 -0.169 if reset Tilting to -56 -56.00 degrees 2.7 -7.3 unbinned pixels; 2.7 0.0 nm along two shift axes; 2.5 1.2 nm on specimen axes Rate = 13.67793 A/sec 6.5 1.6 unbinned pixels; 0.2 2.4 nm along two shift axes; -0.8 2.2 nm on specimen axes Rate = 11.8568 A/sec 3.8 0.8 unbinned pixels; 0.2 1.3 nm along two shift axes; -0.4 1.3 nm on specimen axes Rate = 6.74754 A/sec Drift is low enough after shot 3 Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = 0.693 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_046_-56.0_Oct22_22.50.13.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 64.1 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift 0.195 -0.924 IS units 2694.4 349.6 unbinned pixels; need stage -0.203 1.683 if reset Tilting to -58 -2.3 -5.8 unbinned pixels; 1.6 1.5 nm along two shift axes; 2.1 -0.6 nm on specimen axes Rate = 10.97339 A/sec 2.8 2.7 unbinned pixels; 0.6 1.2 nm along two shift axes; -1.0 0.9 nm on specimen axes Rate = 6.84423 A/sec Drift is low enough after shot 2 Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.02 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.01 nm/sec Defocus = 0.668 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_047_-58.0_Oct22_22.51.15.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 87.1 In SetExposureForMean 90, exposure time changed to 3.3600 Image shift 0.232 -1.000 IS units 2937.2 319.5 unbinned pixels; need stage -0.200 1.940 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -14.0 4.6 unbinned pixels; 3.2 4.1 nm along two shift axes; -1.2 -5.0 nm on specimen axes Rate = 25.76874 A/sec -8.8 1.0 unbinned pixels; 1.4 2.8 nm along two shift axes; -0.1 -3.1 nm on specimen axes Rate = 15.60395 A/sec -8.0 4.2 unbinned pixels; 2.3 2.1 nm along two shift axes; -1.2 -2.9 nm on specimen axes Rate = 15.86815 A/sec -4.8 2.2 unbinned pixels; 1.3 1.3 nm along two shift axes; -0.6 -1.7 nm on specimen axes Rate = 9.18806 A/sec -4.0 1.9 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.6 -1.4 nm on specimen axes Rate = 7.70915 A/sec Drift is low enough after shot 5 Measured defocus = -3.84 microns changed by -0.16 to target drift = 0.11 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.970 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_048_38.0_Oct22_22.52.43.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 140.7 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.250 0.073 IS units -444.4 609.8 unbinned pixels; need stage -0.196 -0.224 if reset Tilting to 40 Correcting tilt backlash -6.1 -6.3 unbinned pixels; 1.4 2.8 nm along two shift axes; 2.4 -1.9 nm on specimen axes Rate = 15.48634 A/sec -3.9 5.8 unbinned pixels; 2.4 0.6 nm along two shift axes; -1.9 -1.5 nm on specimen axes Rate = 12.28555 A/sec -2.3 2.3 unbinned pixels; 1.0 0.5 nm along two shift axes; -0.7 -0.9 nm on specimen axes Rate = 5.70132 A/sec Drift is low enough after shot 3 Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.09 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -3.020 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_049_40.0_Oct22_22.53.55.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 84.0 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.182 -0.033 IS units -90.4 527.2 unbinned pixels; need stage -0.179 -0.064 if reset Tilting to -60 -60.00 degrees 5.7 -5.0 unbinned pixels; 2.3 1.3 nm along two shift axes; 1.6 2.2 nm on specimen axes Rate = 13.36766 A/sec 4.4 2.6 unbinned pixels; 0.4 1.8 nm along two shift axes; -1.1 1.5 nm on specimen axes Rate = 9.04281 A/sec 2.5 1.5 unbinned pixels; 0.2 1.0 nm along two shift axes; -0.6 0.8 nm on specimen axes Rate = 5.09224 A/sec Drift is low enough after shot 3 Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.04 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = 0.750 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_050_-60.0_Oct22_22.55.08.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 59.4 In SetExposureForMean 90, exposure time changed to 3.5200 Image shift 0.262 -1.061 IS units 3132.8 296.6 unbinned pixels; need stage -0.198 2.196 if reset Tilting to -62 -1.0 -4.4 unbinned pixels; 1.3 0.9 nm along two shift axes; 1.6 -0.2 nm on specimen axes Rate = 7.96858 A/sec Drift is low enough after shot 1 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.06 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = 0.683 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_051_-62.0_Oct22_22.56.05.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 85.3 In SetExposureForMean 90, exposure time changed to 4.0000 Image shift 0.290 -1.141 IS units 3376.3 297.4 unbinned pixels; need stage -0.206 2.522 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -17.5 0.5 unbinned pixels; 2.2 5.7 nm along two shift axes; 0.4 -6.1 nm on specimen axes Rate = 30.65223 A/sec -9.2 3.6 unbinned pixels; 2.3 2.6 nm along two shift axes; -1.0 -3.3 nm on specimen axes Rate = 17.34974 A/sec -7.4 2.7 unbinned pixels; 1.8 2.1 nm along two shift axes; -0.7 -2.7 nm on specimen axes Rate = 13.79277 A/sec -6.4 2.5 unbinned pixels; 1.6 1.8 nm along two shift axes; -0.7 -2.3 nm on specimen axes Rate = 12.01444 A/sec -5.1 1.9 unbinned pixels; 1.2 1.5 nm along two shift axes; -0.5 -1.9 nm on specimen axes Rate = 9.56296 A/sec -3.3 1.6 unbinned pixels; 0.9 0.9 nm along two shift axes; -0.5 -1.2 nm on specimen axes Rate = 6.41212 A/sec Drift is low enough after shot 6 Measured defocus = -3.84 microns changed by -0.16 to target drift = 0.11 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -3.189 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_052_42.0_Oct22_22.57.38.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 154.6 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.189 -0.051 IS units -47.2 562.6 unbinned pixels; need stage -0.192 -0.047 if reset Tilting to 44 Correcting tilt backlash -6.1 -2.7 unbinned pixels; 0.2 2.3 nm along two shift axes; 1.1 -2.0 nm on specimen axes Rate = 11.65915 A/sec -2.0 4.0 unbinned pixels; 1.6 0.2 nm along two shift axes; -1.3 -0.8 nm on specimen axes Rate = 7.81319 A/sec Drift is low enough after shot 2 Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.12 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -3.252 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_053_44.0_Oct22_22.58.43.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 88.6 In SetExposureForMean 90, change by a factor of 1.0233 would require too small a change in exposure time Image shift -0.134 -0.146 IS units 265.2 503.4 unbinned pixels; need stage -0.181 0.108 if reset Tilting to -64 -64.00 degrees 7.0 -6.9 unbinned pixels; 3.1 1.5 nm along two shift axes; 2.2 2.7 nm on specimen axes Rate = 17.23381 A/sec 7.5 3.0 unbinned pixels; 0.1 2.8 nm along two shift axes; -1.3 2.5 nm on specimen axes Rate = 14.22422 A/sec 0.4 1.1 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.1 nm on specimen axes Rate = 2.12429 A/sec Drift is low enough after shot 3 Measured defocus = -4.10 microns changed by 0.10 to target drift = 0.08 nm/sec Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.05 nm/sec Defocus = 0.763 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_054_-64.0_Oct22_22.59.58.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 52.4 In SetExposureForMean 90, exposure time changed to 4.4800 Image shift 0.338 -1.217 IS units 3628.5 238.8 unbinned pixels; need stage -0.194 2.909 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash -20.1 0.6 unbinned pixels; 2.5 6.6 nm along two shift axes; 0.4 -7.1 nm on specimen axes Rate = 35.23633 A/sec -9.7 4.5 unbinned pixels; 2.6 2.7 nm along two shift axes; -1.3 -3.5 nm on specimen axes Rate = 18.73094 A/sec -7.1 3.1 unbinned pixels; 1.9 2.0 nm along two shift axes; -0.9 -2.6 nm on specimen axes Rate = 13.56736 A/sec -5.9 2.3 unbinned pixels; 1.4 1.7 nm along two shift axes; -0.6 -2.1 nm on specimen axes Rate = 11.07327 A/sec -4.0 2.2 unbinned pixels; 1.2 1.1 nm along two shift axes; -0.6 -1.5 nm on specimen axes Rate = 8.02692 A/sec -3.3 1.5 unbinned pixels; 0.9 0.9 nm along two shift axes; -0.4 -1.2 nm on specimen axes Rate = 6.38679 A/sec Drift is low enough after shot 6 Measured defocus = -3.85 microns changed by -0.15 to target drift = 0.11 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -3.404 um Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_055_46.0_Oct22_23.01.31.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 156.8 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.138 -0.195 IS units 392.7 562.2 unbinned pixels; need stage -0.206 0.174 if reset Tilting to 48 Correcting tilt backlash -8.0 -2.5 unbinned pixels; 0.1 3.0 nm along two shift axes; 1.1 -2.7 nm on specimen axes Rate = 14.80351 A/sec -5.1 6.1 unbinned pixels; 2.6 0.9 nm along two shift axes; -2.0 -2.0 nm on specimen axes Rate = 13.97955 A/sec -1.8 1.2 unbinned pixels; 0.6 0.5 nm along two shift axes; -0.4 -0.7 nm on specimen axes Rate = 3.87265 A/sec Drift is low enough after shot 3 Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.10 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -3.449 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_03_6_056_48.0_Oct22_23.02.48.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 85.0 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift -0.079 -0.280 IS units 681.8 480.8 unbinned pixels; need stage -0.186 0.337 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -3.31 microns changed by -0.69 to target drift = 0.11 nm/sec Focus area changed from stored state, axis position -2.40 angle 0, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_04.st Running script 1 at item 8 (254.07, -45.46) 23:03:36 Refine ZLP: additional offset = -0.3 eV; total offset = 5.4 eV Item 8: Stage: 254.07 -45.46 -237 Label: 8 Note: Sec 6 - anchor_maps.map Target defocus is: -4.00 um Target defocus is: -4.25 um Target defocus = -4.25 Angle = -4.40, increment = 0.60, shift = -11.9 -6.5 pixels, -0.029 um Y Angle = -3.20, increment = 1.80, shift = -19.8 -9.1 pixels, -0.047 um Y Angle = 0.41, increment = 5.41, shift = -58.4 -18.4 pixels, -0.137 um Y Angle = 3.00, increment = 8.00, shift = -63.3 -23.4 pixels, -0.150 um Y Rough eucentricity: changing Z by 1.07 to -235.65, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = -60.0, 0.5 pixels, 0.016, -0.136 um, IS = -0.016, 0.136 Angle = 11.00, shift = -52.4, -6.7 pixels, -0.002, -0.121 um, IS = -0.015, 0.257 Angle = 4.00, shift = 9.2, 6.0 pixels, 0.011, 0.022 um, IS = -0.026, 0.234 Angle = -4.00, shift = -16.1, -20.9 pixels, -0.043, -0.042 um, IS = 0.017, 0.276 Angle = -11.00, shift = -6.4, -14.2 pixels, -0.031, -0.018 um, IS = 0.048, 0.294 Trimming A by 20 25, reference by 20 25 Angle = -18.00, shift = -29.3, -0.3 pixels, 0.007, -0.067 um, IS = 0.041, 0.361 Angle = -24.00, shift = -6.6, 0.4 pixels, 0.003, -0.015 um, IS = 0.038, 0.376 General solution delZ 0.38, delY 0.62, yZero 0.28 solution with assumed YZero 0.26: delZ 0.38, delY 0.31 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.38 microns and lateral displacement of -0.62 microns Disparity in stage position after aligning to center of map frame: 0.01 0.02 Aligning to target with image shift equivalent to stage shift: 0.01 0.01 Disparity in stage position after aligning to map item itself: -0.01 0.05 (align shift -0.03 0.01) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 254.09 -45.42 -236.02 0.1 10.5 unbinned pixels; 3.5 1.3 nm along two shift axes; -3.7 -0.3 nm on specimen axes Rate = 18.54975 A/sec 0.5 6.7 unbinned pixels; 2.2 1.0 nm along two shift axes; -2.4 -0.0 nm on specimen axes Rate = 11.82487 A/sec 0.3 6.0 unbinned pixels; 2.0 0.8 nm along two shift axes; -2.1 -0.1 nm on specimen axes Rate = 10.6415 A/sec -0.1 5.8 unbinned pixels; 1.9 0.7 nm along two shift axes; -2.0 -0.2 nm on specimen axes Rate = 10.19128 A/sec -0.1 5.9 unbinned pixels; 2.0 0.7 nm along two shift axes; -2.1 -0.2 nm on specimen axes Rate = 10.35251 A/sec 0.1 5.7 unbinned pixels; 1.9 0.7 nm along two shift axes; -2.0 -0.2 nm on specimen axes Rate = 10.14269 A/sec -0.0 5.7 unbinned pixels; 1.9 0.7 nm along two shift axes; -2.0 -0.2 nm on specimen axes Rate = 10.0301 A/sec 0.1 5.7 unbinned pixels; 1.9 0.7 nm along two shift axes; -2.0 -0.1 nm on specimen axes Rate = 10.12515 A/sec 0.3 5.2 unbinned pixels; 1.7 0.7 nm along two shift axes; -1.8 -0.1 nm on specimen axes Rate = 9.19617 A/sec -0.0 5.6 unbinned pixels; 1.8 0.7 nm along two shift axes; -1.9 -0.2 nm on specimen axes Rate = 9.81038 A/sec Drift never got below 8: Skipping ... Measured defocus = -2.96 microns changed by -1.29 to target drift = 0.17 nm/sec Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.17 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.16 nm/sec Defocus = -1.330 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_000_-10.0_Oct22_23.08.14.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 116.6 In SetExposureForMean 90, exposure time changed to 1.2800 Image shift -0.033 -0.033 IS units 56.8 120.3 unbinned pixels; need stage -0.043 0.016 if reset Tilting to -8 Correcting tilt backlash 11.5 1.6 unbinned pixels; 0.8 4.0 nm along two shift axes; -0.9 4.0 nm on specimen axes Rate = 20.43519 A/sec 4.8 5.6 unbinned pixels; 1.3 2.3 nm along two shift axes; -2.1 1.5 nm on specimen axes Rate = 13.02557 A/sec 3.0 2.3 unbinned pixels; 0.4 1.3 nm along two shift axes; -0.9 1.0 nm on specimen axes Rate = 6.60184 A/sec Drift is low enough after shot 3 Measured defocus = -4.14 microns changed by -0.11 to target drift = 0.10 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.10 nm/sec Defocus = -1.435 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_001_-8.0_Oct22_23.09.23.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 93.6 In SetExposureForMean 90, change by a factor of 0.9611 would require too small a change in exposure time Image shift -0.102 -0.036 IS units -2.6 312.6 unbinned pixels; need stage -0.107 -0.011 if reset Tilting to -12 -12.00 degrees -5.2 7.5 unbinned pixels; 3.1 0.8 nm along two shift axes; -2.5 -2.0 nm on specimen axes Rate = 15.92258 A/sec -1.0 5.2 unbinned pixels; 1.8 0.3 nm along two shift axes; -1.8 -0.5 nm on specimen axes Rate = 9.34983 A/sec -1.4 2.5 unbinned pixels; 1.0 0.1 nm along two shift axes; -0.8 -0.6 nm on specimen axes Rate = 5.02428 A/sec Drift is low enough after shot 3 Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -1.282 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_002_-12.0_Oct22_23.10.29.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 92.8 In SetExposureForMean 90, change by a factor of 0.9692 would require too small a change in exposure time Image shift -0.155 0.017 IS units -198.3 407.4 unbinned pixels; need stage -0.134 -0.085 if reset Tilting to -14 2.9 3.2 unbinned pixels; 0.7 1.3 nm along two shift axes; -1.2 0.9 nm on specimen axes Rate = 7.54501 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.10 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -1.180 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_003_-14.0_Oct22_23.11.25.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 92.4 In SetExposureForMean 90, change by a factor of 0.9733 would require too small a change in exposure time Image shift -0.225 0.132 IS units -580.6 486.7 unbinned pixels; need stage -0.150 -0.228 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 8.6 -0.7 unbinned pixels; 1.2 2.8 nm along two shift axes; -0.0 3.0 nm on specimen axes Rate = 15.16878 A/sec 7.3 1.6 unbinned pixels; 0.3 2.6 nm along two shift axes; -0.8 2.5 nm on specimen axes Rate = 13.19109 A/sec 4.4 2.2 unbinned pixels; 0.2 1.7 nm along two shift axes; -0.9 1.5 nm on specimen axes Rate = 8.64242 A/sec 2.8 1.5 unbinned pixels; 0.2 1.1 nm along two shift axes; -0.6 0.9 nm on specimen axes Rate = 5.57552 A/sec Drift is low enough after shot 4 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -1.508 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_004_-6.0_Oct22_23.12.39.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 94.0 In SetExposureForMean 90, change by a factor of 0.9576 would require too small a change in exposure time Image shift -0.109 -0.139 IS units 269.5 428.7 unbinned pixels; need stage -0.156 0.082 if reset Tilting to -4 Correcting tilt backlash -6.4 -0.1 unbinned pixels; 0.7 2.1 nm along two shift axes; 0.2 -2.2 nm on specimen axes Rate = 11.27845 A/sec -0.6 2.8 unbinned pixels; 1.0 0.1 nm along two shift axes; -1.0 -0.3 nm on specimen axes Rate = 4.97393 A/sec Drift is low enough after shot 2 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.07 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -1.612 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_005_-4.0_Oct22_23.13.42.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 94.0 In SetExposureForMean 90, change by a factor of 0.9574 would require too small a change in exposure time Image shift -0.101 -0.173 IS units 370.2 439.2 unbinned pixels; need stage -0.163 0.117 if reset Tilting to -16 -16.00 degrees -7.6 1.7 unbinned pixels; 1.4 2.3 nm along two shift axes; -0.4 -2.7 nm on specimen axes Rate = 13.68681 A/sec -0.4 2.9 unbinned pixels; 1.0 0.2 nm along two shift axes; -1.0 -0.2 nm on specimen axes Rate = 5.18765 A/sec Drift is low enough after shot 2 Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -1.142 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_006_-16.0_Oct22_23.14.45.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 91.8 In SetExposureForMean 90, change by a factor of 0.9795 would require too small a change in exposure time Image shift -0.251 0.087 IS units -481.8 601.1 unbinned pixels; need stage -0.192 -0.197 if reset Tilting to -18 2.9 2.3 unbinned pixels; 0.4 1.2 nm along two shift axes; -0.9 0.9 nm on specimen axes Rate = 6.5382 A/sec Drift is low enough after shot 1 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.11 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -1.061 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_007_-18.0_Oct22_23.15.40.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 91.1 In SetExposureForMean 90, change by a factor of 0.9869 would require too small a change in exposure time Image shift -0.275 0.121 IS units -599.4 634.4 unbinned pixels; need stage -0.200 -0.244 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 8.6 1.5 unbinned pixels; 0.5 3.0 nm along two shift axes; -0.8 3.0 nm on specimen axes Rate = 15.41818 A/sec 7.7 3.3 unbinned pixels; 0.2 3.0 nm along two shift axes; -1.4 2.6 nm on specimen axes Rate = 14.86203 A/sec 4.6 2.3 unbinned pixels; 0.2 1.8 nm along two shift axes; -0.9 1.6 nm on specimen axes Rate = 9.12829 A/sec 2.6 1.4 unbinned pixels; 0.2 1.0 nm along two shift axes; -0.6 0.9 nm on specimen axes Rate = 5.20635 A/sec Drift is low enough after shot 4 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.08 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -1.710 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_008_-2.0_Oct22_23.16.56.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 94.0 In SetExposureForMean 90, change by a factor of 0.9573 would require too small a change in exposure time Image shift -0.158 -0.160 IS units 278.1 583.3 unbinned pixels; need stage -0.209 0.079 if reset Tilting to 0 Correcting tilt backlash -6.7 -1.7 unbinned pixels; 0.2 2.4 nm along two shift axes; 0.8 -2.3 nm on specimen axes Rate = 12.21836 A/sec -2.0 3.8 unbinned pixels; 1.5 0.2 nm along two shift axes; -1.3 -0.8 nm on specimen axes Rate = 7.4775 A/sec Drift is low enough after shot 2 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.08 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -1.811 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_009_-0.0_Oct22_23.17.58.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 94.1 In SetExposureForMean 90, change by a factor of 0.9567 would require too small a change in exposure time Image shift -0.141 -0.204 IS units 412.9 577.0 unbinned pixels; need stage -0.211 0.127 if reset Tilting to -20 -20.00 degrees -6.9 0.3 unbinned pixels; 0.9 2.3 nm along two shift axes; 0.1 -2.4 nm on specimen axes Rate = 12.12973 A/sec 1.2 2.7 unbinned pixels; 0.8 0.7 nm along two shift axes; -1.0 0.3 nm on specimen axes Rate = 5.20087 A/sec Drift is low enough after shot 2 Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -1.062 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_010_-20.0_Oct22_23.19.02.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 90.4 In SetExposureForMean 90, change by a factor of 0.9950 would require too small a change in exposure time Image shift -0.289 0.057 IS units -437.8 733.0 unbinned pixels; need stage -0.239 -0.190 if reset Tilting to -22 2.2 0.7 unbinned pixels; 0.0 0.8 nm along two shift axes; -0.3 0.8 nm on specimen axes Rate = 4.07977 A/sec Drift is low enough after shot 1 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.08 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -0.981 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_011_-22.0_Oct22_23.19.57.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 89.7 In SetExposureForMean 90, change by a factor of 1.0019 would require too small a change in exposure time Image shift -0.300 0.071 IS units -488.2 750.6 unbinned pixels; need stage -0.243 -0.212 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 8.6 0.7 unbinned pixels; 0.8 2.9 nm along two shift axes; -0.5 3.0 nm on specimen axes Rate = 15.06944 A/sec 5.9 5.8 unbinned pixels; 1.2 2.6 nm along two shift axes; -2.2 1.9 nm on specimen axes Rate = 14.5642 A/sec 2.4 1.3 unbinned pixels; 0.2 1.0 nm along two shift axes; -0.5 0.8 nm on specimen axes Rate = 4.88412 A/sec Drift is low enough after shot 3 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.07 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -1.940 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_012_2.0_Oct22_23.21.12.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 94.2 In SetExposureForMean 90, change by a factor of 0.9557 would require too small a change in exposure time Image shift -0.206 -0.159 IS units 227.7 711.7 unbinned pixels; need stage -0.252 0.057 if reset Tilting to 4 Correcting tilt backlash -9.9 -2.1 unbinned pixels; 0.5 3.5 nm along two shift axes; 1.0 -3.4 nm on specimen axes Rate = 17.83843 A/sec -5.5 3.6 unbinned pixels; 1.8 1.4 nm along two shift axes; -1.1 -2.0 nm on specimen axes Rate = 11.48985 A/sec -4.3 2.3 unbinned pixels; 1.3 1.1 nm along two shift axes; -0.7 -1.6 nm on specimen axes Rate = 8.54091 A/sec -3.8 2.4 unbinned pixels; 1.2 1.0 nm along two shift axes; -0.7 -1.4 nm on specimen axes Rate = 7.84526 A/sec Drift is low enough after shot 4 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.041 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_013_4.0_Oct22_23.22.32.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 94.0 In SetExposureForMean 90, change by a factor of 0.9573 would require too small a change in exposure time Image shift -0.189 -0.192 IS units 333.9 698.6 unbinned pixels; need stage -0.251 0.096 if reset Tilting to -24 -24.00 degrees -4.3 -0.4 unbinned pixels; 0.4 1.5 nm along two shift axes; 0.3 -1.5 nm on specimen axes Rate = 7.61597 A/sec Drift is low enough after shot 1 Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.04 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -0.959 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_014_-24.0_Oct22_23.23.30.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 88.7 In SetExposureForMean 90, change by a factor of 1.0137 would require too small a change in exposure time Image shift -0.306 0.012 IS units -332.9 821.1 unbinned pixels; need stage -0.272 -0.158 if reset Tilting to -26 4.7 0.1 unbinned pixels; 0.5 1.6 nm along two shift axes; -0.2 1.6 nm on specimen axes Rate = 8.20921 A/sec 2.2 0.5 unbinned pixels; 0.1 0.8 nm along two shift axes; -0.3 0.8 nm on specimen axes Rate = 3.9875 A/sec Drift is low enough after shot 2 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.05 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.884 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_015_-26.0_Oct22_23.24.31.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 87.8 In SetExposureForMean 90, change by a factor of 1.0237 would require too small a change in exposure time Image shift -0.304 0.002 IS units -305.8 826.4 unbinned pixels; need stage -0.275 -0.150 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 4.4 1.9 unbinned pixels; 0.1 1.7 nm along two shift axes; -0.8 1.5 nm on specimen axes Rate = 8.46387 A/sec 3.5 3.1 unbinned pixels; 0.6 1.5 nm along two shift axes; -1.2 1.1 nm on specimen axes Rate = 8.29915 A/sec 1.5 1.8 unbinned pixels; 0.4 0.7 nm along two shift axes; -0.7 0.5 nm on specimen axes Rate = 4.18891 A/sec Drift is low enough after shot 3 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.03 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -2.139 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_016_6.0_Oct22_23.25.44.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 93.8 In SetExposureForMean 90, change by a factor of 0.9591 would require too small a change in exposure time Image shift -0.247 -0.147 IS units 153.9 812.7 unbinned pixels; need stage -0.284 0.028 if reset Tilting to 8 Correcting tilt backlash -8.8 -2.6 unbinned pixels; 0.2 3.2 nm along two shift axes; 1.2 -3.0 nm on specimen axes Rate = 16.09886 A/sec -3.6 4.5 unbinned pixels; 1.9 0.6 nm along two shift axes; -1.5 -1.4 nm on specimen axes Rate = 10.12307 A/sec -4.0 1.1 unbinned pixels; 0.8 1.2 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 7.31472 A/sec Drift is low enough after shot 3 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -2.237 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_017_8.0_Oct22_23.26.55.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 93.4 In SetExposureForMean 90, change by a factor of 0.9636 would require too small a change in exposure time Image shift -0.233 -0.176 IS units 247.4 801.6 unbinned pixels; need stage -0.283 0.062 if reset Tilting to -28 -28.00 degrees -3.4 0.4 unbinned pixels; 0.5 1.1 nm along two shift axes; -0.0 -1.2 nm on specimen axes Rate = 6.02399 A/sec Drift is low enough after shot 1 Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.02 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.859 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_018_-28.0_Oct22_23.27.54.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 86.4 In SetExposureForMean 90, change by a factor of 1.0401 would require too small a change in exposure time Image shift -0.319 -0.027 IS units -240.5 895.1 unbinned pixels; need stage -0.301 -0.129 if reset Tilting to -30 1.3 -1.5 unbinned pixels; 0.6 0.3 nm along two shift axes; 0.5 0.5 nm on specimen axes Rate = 3.45734 A/sec Drift is low enough after shot 1 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.06 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.782 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_019_-30.0_Oct22_23.28.49.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 85.0 In SetExposureForMean 90, change by a factor of 1.0573 would require too small a change in exposure time Image shift -0.307 -0.058 IS units -146.6 890.6 unbinned pixels; need stage -0.302 -0.093 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees 6.0 1.7 unbinned pixels; 0.1 2.2 nm along two shift axes; -0.8 2.1 nm on specimen axes Rate = 11.02744 A/sec 2.5 2.5 unbinned pixels; 0.5 1.1 nm along two shift axes; -1.0 0.8 nm on specimen axes Rate = 6.29971 A/sec Drift is low enough after shot 2 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.04 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -2.329 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_020_10.0_Oct22_23.30.01.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 93.1 In SetExposureForMean 90, change by a factor of 0.9662 would require too small a change in exposure time Image shift -0.269 -0.164 IS units 179.0 887.4 unbinned pixels; need stage -0.311 0.035 if reset Tilting to 12 Correcting tilt backlash -15.5 2.8 unbinned pixels; 2.7 4.8 nm along two shift axes; -0.5 -5.5 nm on specimen axes Rate = 27.67262 A/sec -10.2 3.3 unbinned pixels; 2.3 3.0 nm along two shift axes; -0.9 -3.7 nm on specimen axes Rate = 18.73528 A/sec -7.6 2.1 unbinned pixels; 1.6 2.3 nm along two shift axes; -0.5 -2.7 nm on specimen axes Rate = 13.84345 A/sec -6.0 2.0 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.2 nm on specimen axes Rate = 11.14497 A/sec -4.4 1.5 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.21096 A/sec -3.9 1.8 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.5 -1.4 nm on specimen axes Rate = 7.57536 A/sec Drift is low enough after shot 6 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.09 nm/sec Defocus = -2.416 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_021_12.0_Oct22_23.31.27.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 92.6 In SetExposureForMean 90, change by a factor of 0.9722 would require too small a change in exposure time Image shift -0.276 -0.148 IS units 129.1 890.9 unbinned pixels; need stage -0.311 0.017 if reset Tilting to -32 -32.00 degrees -4.2 -1.4 unbinned pixels; 0.0 1.6 nm along two shift axes; 0.6 -1.4 nm on specimen axes Rate = 7.85972 A/sec Drift is low enough after shot 1 Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.02 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.743 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_022_-32.0_Oct22_23.32.27.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 83.6 In SetExposureForMean 90, exposure time changed to 1.4400 Image shift -0.299 -0.130 IS units 57.7 937.6 unbinned pixels; need stage -0.324 -0.012 if reset Tilting to -34 1.8 -4.8 unbinned pixels; 1.8 0.0 nm along two shift axes; 1.6 0.8 nm on specimen axes Rate = 9.07659 A/sec 2.8 1.8 unbinned pixels; 0.3 1.2 nm along two shift axes; -0.7 0.9 nm on specimen axes Rate = 5.92428 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.02 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.678 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_023_-34.0_Oct22_23.33.27.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 92.5 In SetExposureForMean 90, change by a factor of 0.9704 would require too small a change in exposure time Image shift -0.272 -0.185 IS units 234.0 915.9 unbinned pixels; need stage -0.322 0.064 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -4.3 2.0 unbinned pixels; 1.2 1.2 nm along two shift axes; -0.6 -1.6 nm on specimen axes Rate = 8.367 A/sec -2.8 2.5 unbinned pixels; 1.2 0.6 nm along two shift axes; -0.8 -1.0 nm on specimen axes Rate = 6.51737 A/sec Drift is low enough after shot 2 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.08 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.488 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_024_14.0_Oct22_23.34.34.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 103.7 In SetExposureForMean 90, exposure time changed to 1.2800 Image shift -0.287 -0.154 IS units 133.8 927.5 unbinned pixels; need stage -0.323 0.018 if reset Tilting to 16 Correcting tilt backlash -7.0 -5.7 unbinned pixels; 1.1 3.0 nm along two shift axes; 2.2 -2.3 nm on specimen axes Rate = 15.8781 A/sec -0.6 4.8 unbinned pixels; 1.7 0.4 nm along two shift axes; -1.7 -0.4 nm on specimen axes Rate = 8.5712 A/sec -3.0 2.2 unbinned pixels; 1.1 0.7 nm along two shift axes; -0.7 -1.1 nm on specimen axes Rate = 6.56152 A/sec Drift is low enough after shot 3 Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.07 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -2.564 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_025_16.0_Oct22_23.35.43.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 91.6 In SetExposureForMean 90, change by a factor of 0.9826 would require too small a change in exposure time Image shift -0.247 -0.222 IS units 357.6 884.4 unbinned pixels; need stage -0.315 0.102 if reset Tilting to -36 -36.00 degrees -0.4 -4.5 unbinned pixels; 1.4 0.7 nm along two shift axes; 1.6 0.0 nm on specimen axes Rate = 7.91534 A/sec Drift is low enough after shot 1 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.04 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.606 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_026_-36.0_Oct22_23.36.43.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 80.5 In SetExposureForMean 90, exposure time changed to 1.4400 Image shift -0.233 -0.294 IS units 566.5 914.7 unbinned pixels; need stage -0.332 0.211 if reset Tilting to -38 2.8 -5.1 unbinned pixels; 2.0 0.3 nm along two shift axes; 1.7 1.2 nm on specimen axes Rate = 10.30783 A/sec 3.5 2.4 unbinned pixels; 0.4 1.4 nm along two shift axes; -0.9 1.1 nm on specimen axes Rate = 7.40434 A/sec Drift is low enough after shot 2 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.05 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.526 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_027_-38.0_Oct22_23.37.44.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 88.8 In SetExposureForMean 90, change by a factor of 1.0100 would require too small a change in exposure time Image shift -0.205 -0.349 IS units 742.5 891.3 unbinned pixels; need stage -0.330 0.296 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -17.4 4.6 unbinned pixels; 3.6 5.2 nm along two shift axes; -1.1 -6.2 nm on specimen axes Rate = 31.55804 A/sec -10.3 3.3 unbinned pixels; 2.3 3.0 nm along two shift axes; -0.8 -3.7 nm on specimen axes Rate = 18.97841 A/sec -7.3 2.0 unbinned pixels; 1.5 2.2 nm along two shift axes; -0.5 -2.6 nm on specimen axes Rate = 13.27076 A/sec -4.6 1.6 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.58316 A/sec -3.5 1.5 unbinned pixels; 0.9 1.0 nm along two shift axes; -0.4 -1.3 nm on specimen axes Rate = 6.66215 A/sec Drift is low enough after shot 5 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -2.654 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_028_18.0_Oct22_23.39.08.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 102.3 In SetExposureForMean 90, exposure time changed to 1.2800 Image shift -0.258 -0.225 IS units 354.9 917.0 unbinned pixels; need stage -0.326 0.101 if reset Tilting to 20 Correcting tilt backlash 11.8 -4.9 unbinned pixels; 3.0 3.3 nm along two shift axes; 1.4 4.3 nm on specimen axes Rate = 22.44125 A/sec 9.0 5.1 unbinned pixels; 0.7 3.6 nm along two shift axes; -2.1 3.0 nm on specimen axes Rate = 18.21141 A/sec 4.2 2.4 unbinned pixels; 0.3 1.7 nm along two shift axes; -1.0 1.4 nm on specimen axes Rate = 8.48642 A/sec 2.7 1.7 unbinned pixels; 0.3 1.1 nm along two shift axes; -0.7 0.9 nm on specimen axes Rate = 5.66976 A/sec Drift is low enough after shot 4 Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.07 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -2.659 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_029_20.0_Oct22_23.40.21.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 90.4 In SetExposureForMean 90, change by a factor of 0.9952 would require too small a change in exposure time Image shift -0.204 -0.353 IS units 756.8 890.8 unbinned pixels; need stage -0.330 0.254 if reset Tilting to -40 -40.00 degrees -0.4 -2.7 unbinned pixels; 0.8 0.4 nm along two shift axes; 1.0 -0.0 nm on specimen axes Rate = 4.7859 A/sec Drift is low enough after shot 1 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.06 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -0.460 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_030_-40.0_Oct22_23.41.22.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 77.1 In SetExposureForMean 90, exposure time changed to 1.4400 Image shift -0.185 -0.438 IS units 1004.9 920.4 unbinned pixels; need stage -0.348 0.425 if reset Tilting to -42 0.8 -5.1 unbinned pixels; 1.8 0.4 nm along two shift axes; 1.8 0.4 nm on specimen axes Rate = 9.17076 A/sec 2.5 2.6 unbinned pixels; 0.6 1.1 nm along two shift axes; -1.0 0.8 nm on specimen axes Rate = 6.34129 A/sec Drift is low enough after shot 2 Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.02 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.410 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_031_-42.0_Oct22_23.42.28.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 84.4 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.154 -0.504 IS units 1213.4 900.3 unbinned pixels; need stage -0.347 0.538 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -16.5 3.2 unbinned pixels; 3.0 5.1 nm along two shift axes; -0.6 -5.9 nm on specimen axes Rate = 29.52853 A/sec -7.2 3.2 unbinned pixels; 1.9 2.0 nm along two shift axes; -0.9 -2.6 nm on specimen axes Rate = 13.81264 A/sec -6.8 2.0 unbinned pixels; 1.5 2.0 nm along two shift axes; -0.5 -2.5 nm on specimen axes Rate = 12.45545 A/sec -4.7 1.6 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.65964 A/sec -3.8 1.6 unbinned pixels; 1.0 1.1 nm along two shift axes; -0.4 -1.4 nm on specimen axes Rate = 7.24903 A/sec Drift is low enough after shot 5 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -2.734 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_032_22.0_Oct22_23.43.53.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 111.5 In SetExposureForMean 90, exposure time changed to 1.2800 Image shift -0.228 -0.307 IS units 606.2 911.7 unbinned pixels; need stage -0.332 0.199 if reset Tilting to 24 Correcting tilt backlash -0.4 -2.8 unbinned pixels; 0.9 0.5 nm along two shift axes; 1.0 -0.1 nm on specimen axes Rate = 4.94009 A/sec Drift is low enough after shot 1 Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.15 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -2.725 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_033_24.0_Oct22_23.44.51.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 88.4 In SetExposureForMean 90, change by a factor of 1.0170 would require too small a change in exposure time Image shift -0.169 -0.448 IS units 1046.5 887.6 unbinned pixels; need stage -0.338 0.374 if reset Tilting to -44 -44.00 degrees -1.2 -3.4 unbinned pixels; 1.0 0.8 nm along two shift axes; 1.2 -0.3 nm on specimen axes Rate = 6.3903 A/sec Drift is low enough after shot 1 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.06 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -0.364 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_034_-44.0_Oct22_23.45.55.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 72.7 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.130 -0.586 IS units 1458.5 910.6 unbinned pixels; need stage -0.358 0.676 if reset Tilting to -46 1.9 -2.1 unbinned pixels; 0.9 0.4 nm along two shift axes; 0.7 0.7 nm on specimen axes Rate = 4.96881 A/sec Drift is low enough after shot 1 Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.07 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.308 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_035_-46.0_Oct22_23.46.52.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 88.1 In SetExposureForMean 90, change by a factor of 1.0149 would require too small a change in exposure time Image shift -0.108 -0.626 IS units 1589.8 890.6 unbinned pixels; need stage -0.355 0.768 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -13.0 6.7 unbinned pixels; 3.7 3.5 nm along two shift axes; -1.9 -4.8 nm on specimen axes Rate = 25.63282 A/sec -8.0 2.4 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.6 -2.9 nm on specimen axes Rate = 14.57375 A/sec -8.1 1.5 unbinned pixels; 1.4 2.5 nm along two shift axes; -0.3 -2.9 nm on specimen axes Rate = 14.44373 A/sec -2.9 2.3 unbinned pixels; 1.1 0.7 nm along two shift axes; -0.7 -1.1 nm on specimen axes Rate = 6.3789 A/sec Drift is low enough after shot 4 Measured defocus = -4.14 microns changed by -0.11 to target drift = 0.10 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.833 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_036_26.0_Oct22_23.48.13.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 109.1 In SetExposureForMean 90, exposure time changed to 1.2800 Image shift -0.198 -0.414 IS units 928.1 932.9 unbinned pixels; need stage -0.350 0.331 if reset Tilting to 28 Correcting tilt backlash -2.1 -1.0 unbinned pixels; 0.1 0.8 nm along two shift axes; 0.4 -0.7 nm on specimen axes Rate = 4.12957 A/sec Drift is low enough after shot 1 Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.14 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -2.833 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_037_28.0_Oct22_23.49.12.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 86.1 In SetExposureForMean 90, change by a factor of 1.0446 would require too small a change in exposure time Image shift -0.145 -0.543 IS units 1327.2 911.1 unbinned pixels; need stage -0.354 0.498 if reset Tilting to -48 -48.00 degrees 1.3 -4.1 unbinned pixels; 1.5 0.0 nm along two shift axes; 1.4 0.6 nm on specimen axes Rate = 7.57518 A/sec Drift is low enough after shot 1 Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.04 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.287 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_038_-48.0_Oct22_23.50.13.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 67.8 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.072 -0.733 IS units 1916.1 894.1 unbinned pixels; need stage -0.367 0.969 if reset Tilting to -50 0.0 -5.8 unbinned pixels; 1.9 0.7 nm along two shift axes; 2.0 0.2 nm on specimen axes Rate = 10.32195 A/sec 3.6 2.2 unbinned pixels; 0.3 1.4 nm along two shift axes; -0.9 1.2 nm on specimen axes Rate = 7.34563 A/sec Drift is low enough after shot 2 Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.02 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.290 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_039_-50.0_Oct22_23.51.16.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 89.2 In SetExposureForMean 90, change by a factor of 0.9985 would require too small a change in exposure time Image shift -0.035 -0.802 IS units 2138.7 858.1 unbinned pixels; need stage -0.361 1.133 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -15.0 6.2 unbinned pixels; 3.8 4.2 nm along two shift axes; -1.7 -5.4 nm on specimen axes Rate = 28.4265 A/sec -4.8 5.4 unbinned pixels; 2.4 0.9 nm along two shift axes; -1.7 -1.9 nm on specimen axes Rate = 12.69359 A/sec -5.4 2.2 unbinned pixels; 1.4 1.5 nm along two shift axes; -0.6 -2.0 nm on specimen axes Rate = 10.23275 A/sec -4.7 2.0 unbinned pixels; 1.2 1.3 nm along two shift axes; -0.6 -1.7 nm on specimen axes Rate = 8.9857 A/sec -5.6 1.8 unbinned pixels; 1.2 1.6 nm along two shift axes; -0.5 -2.0 nm on specimen axes Rate = 10.32374 A/sec -3.0 1.1 unbinned pixels; 0.7 0.9 nm along two shift axes; -0.3 -1.1 nm on specimen axes Rate = 5.64944 A/sec Drift is low enough after shot 6 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.06 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.962 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_040_30.0_Oct22_23.52.54.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 116.7 In SetExposureForMean 90, exposure time changed to 1.2800 WARNING: Desired exposure time (1.356) differs from actual one (1.280) by more than 5% Image shift -0.186 -0.448 IS units 1030.6 933.8 unbinned pixels; need stage -0.353 0.386 if reset Tilting to 32 Correcting tilt backlash -6.5 0.2 unbinned pixels; 0.8 2.1 nm along two shift axes; 0.1 -2.3 nm on specimen axes Rate = 11.45469 A/sec -2.9 4.5 unbinned pixels; 1.8 0.4 nm along two shift axes; -1.5 -1.2 nm on specimen axes Rate = 9.47966 A/sec -2.4 2.5 unbinned pixels; 1.1 0.5 nm along two shift axes; -0.8 -0.9 nm on specimen axes Rate = 6.13503 A/sec Drift is low enough after shot 3 Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.974 um Dewars are NOT busy filling 8 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_041_32.0_Oct22_23.54.08.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 83.6 In SetExposureForMean 90, exposure time changed to 1.4400 Image shift -0.127 -0.556 IS units 1381.8 876.1 unbinned pixels; need stage -0.344 0.543 if reset Tilting to -52 -52.00 degrees 3.5 -4.4 unbinned pixels; 1.9 0.6 nm along two shift axes; 1.4 1.4 nm on specimen axes Rate = 9.83838 A/sec 2.9 2.6 unbinned pixels; 0.5 1.3 nm along two shift axes; -1.0 0.9 nm on specimen axes Rate = 6.92967 A/sec Drift is low enough after shot 2 Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.03 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.252 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_042_-52.0_Oct22_23.55.20.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 69.8 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift 0.006 -0.903 IS units 2451.0 843.7 unbinned pixels; need stage -0.366 1.363 if reset Tilting to -54 1.3 -1.9 unbinned pixels; 0.8 0.2 nm along two shift axes; 0.6 0.5 nm on specimen axes Rate = 4.03181 A/sec Drift is low enough after shot 1 Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.04 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.01 nm/sec Defocus = -0.249 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_043_-54.0_Oct22_23.56.16.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 80.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift 0.027 -0.941 IS units 2576.2 821.0 unbinned pixels; need stage -0.362 1.504 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -12.1 5.9 unbinned pixels; 3.4 3.3 nm along two shift axes; -1.7 -4.4 nm on specimen axes Rate = 23.47173 A/sec -8.0 3.1 unbinned pixels; 1.9 2.3 nm along two shift axes; -0.8 -2.9 nm on specimen axes Rate = 14.92282 A/sec -6.2 2.2 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.6 -2.2 nm on specimen axes Rate = 11.53908 A/sec -4.8 1.5 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.7553 A/sec -3.4 1.4 unbinned pixels; 0.9 1.0 nm along two shift axes; -0.4 -1.2 nm on specimen axes Rate = 6.40798 A/sec Drift is low enough after shot 5 Measured defocus = -4.09 microns changed by -0.16 to target drift = 0.10 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -3.128 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_044_34.0_Oct22_23.57.43.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 123.1 In SetExposureForMean 90, exposure time changed to 1.4400 Image shift -0.140 -0.545 IS units 1338.3 901.2 unbinned pixels; need stage -0.351 0.536 if reset Tilting to 36 Correcting tilt backlash -8.2 -1.1 unbinned pixels; 0.6 2.8 nm along two shift axes; 0.6 -2.8 nm on specimen axes Rate = 14.53061 A/sec -2.7 5.1 unbinned pixels; 2.0 0.3 nm along two shift axes; -1.7 -1.1 nm on specimen axes Rate = 10.0772 A/sec -2.0 2.3 unbinned pixels; 1.0 0.4 nm along two shift axes; -0.8 -0.8 nm on specimen axes Rate = 5.36474 A/sec Drift is low enough after shot 3 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.07 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -3.163 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_045_36.0_Oct22_23.58.54.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 90.6 In SetExposureForMean 90, change by a factor of 0.9920 would require too small a change in exposure time Image shift -0.092 -0.646 IS units 1658.4 865.6 unbinned pixels; need stage -0.349 0.690 if reset Tilting to -56 -56.00 degrees 2.3 -5.3 unbinned pixels; 2.0 0.1 nm along two shift axes; 1.8 1.0 nm on specimen axes Rate = 10.10229 A/sec 4.7 3.2 unbinned pixels; 0.5 1.9 nm along two shift axes; -1.3 1.6 nm on specimen axes Rate = 10.03929 A/sec 1.9 1.1 unbinned pixels; 0.1 0.8 nm along two shift axes; -0.4 0.6 nm on specimen axes Rate = 3.85375 A/sec Drift is low enough after shot 3 Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.04 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -0.238 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_046_-56.0_Oct23_00.00.08.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 61.1 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift 0.058 -1.027 IS units 2840.3 818.6 unbinned pixels; need stage -0.369 1.749 if reset Tilting to -58 -0.1 -3.0 unbinned pixels; 1.0 0.4 nm along two shift axes; 1.1 0.0 nm on specimen axes Rate = 5.31068 A/sec Drift is low enough after shot 1 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.07 nm/sec Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.05 nm/sec Defocus = -0.247 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_047_-58.0_Oct23_00.01.03.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 79.6 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift 0.085 -1.078 IS units 3004.6 794.2 unbinned pixels; need stage -0.366 1.956 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -13.8 2.5 unbinned pixels; 2.4 4.3 nm along two shift axes; -0.5 -4.9 nm on specimen axes Rate = 24.52672 A/sec -9.3 2.5 unbinned pixels; 1.9 2.8 nm along two shift axes; -0.6 -3.3 nm on specimen axes Rate = 16.86897 A/sec -8.0 2.6 unbinned pixels; 1.8 2.3 nm along two shift axes; -0.7 -2.9 nm on specimen axes Rate = 14.76271 A/sec -5.7 2.0 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 10.67673 A/sec -4.3 1.7 unbinned pixels; 1.1 1.2 nm along two shift axes; -0.5 -1.6 nm on specimen axes Rate = 8.14665 A/sec -3.9 1.9 unbinned pixels; 1.1 1.0 nm along two shift axes; -0.6 -1.4 nm on specimen axes Rate = 7.56945 A/sec Drift is low enough after shot 6 Measured defocus = -4.11 microns changed by -0.14 to target drift = 0.10 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -3.314 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_048_38.0_Oct23_00.02.40.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 137.8 In SetExposureForMean 90, exposure time changed to 1.4400 Image shift -0.096 -0.642 IS units 1644.8 872.1 unbinned pixels; need stage -0.351 0.702 if reset Tilting to 40 Correcting tilt backlash -9.6 -2.1 unbinned pixels; 0.4 3.4 nm along two shift axes; 1.0 -3.3 nm on specimen axes Rate = 17.31877 A/sec -3.9 4.2 unbinned pixels; 1.9 0.8 nm along two shift axes; -1.3 -1.5 nm on specimen axes Rate = 10.07187 A/sec -2.4 1.9 unbinned pixels; 0.9 0.6 nm along two shift axes; -0.6 -0.9 nm on specimen axes Rate = 5.47424 A/sec Drift is low enough after shot 3 Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.07 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -3.316 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_049_40.0_Oct23_00.03.50.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 86.3 In SetExposureForMean 90, change by a factor of 1.0407 would require too small a change in exposure time Image shift -0.045 -0.743 IS units 1969.6 830.1 unbinned pixels; need stage -0.346 0.874 if reset Tilting to -60 -60.00 degrees 0.7 -4.4 unbinned pixels; 1.5 0.3 nm along two shift axes; 1.5 0.4 nm on specimen axes Rate = 7.85052 A/sec Drift is low enough after shot 1 Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.10 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -0.242 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_050_-60.0_Oct23_00.04.52.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 48.5 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift 0.112 -1.155 IS units 3240.2 793.6 unbinned pixels; need stage -0.373 2.240 if reset Tilting to -62 -0.3 -0.1 unbinned pixels; 0.0 0.1 nm along two shift axes; 0.0 -0.1 nm on specimen axes Rate = 0.59873 A/sec Drift is low enough after shot 1 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -0.258 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_051_-62.0_Oct23_00.05.48.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 69.5 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift 0.161 -1.253 IS units 3553.1 755.6 unbinned pixels; need stage -0.369 2.624 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -12.7 3.6 unbinned pixels; 2.7 3.8 nm along two shift axes; -0.9 -4.6 nm on specimen axes Rate = 23.17721 A/sec -10.8 2.8 unbinned pixels; 2.2 3.2 nm along two shift axes; -0.7 -3.9 nm on specimen axes Rate = 19.53561 A/sec -8.5 2.6 unbinned pixels; 1.8 2.5 nm along two shift axes; -0.6 -3.1 nm on specimen axes Rate = 15.56671 A/sec -6.6 2.0 unbinned pixels; 1.4 1.9 nm along two shift axes; -0.5 -2.4 nm on specimen axes Rate = 11.99534 A/sec -5.3 1.7 unbinned pixels; 1.2 1.5 nm along two shift axes; -0.4 -1.9 nm on specimen axes Rate = 9.71534 A/sec -4.0 1.5 unbinned pixels; 1.0 1.1 nm along two shift axes; -0.4 -1.4 nm on specimen axes Rate = 7.4678 A/sec Drift is low enough after shot 6 Measured defocus = -4.09 microns changed by -0.16 to target drift = 0.11 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.10 nm/sec Defocus = -3.482 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_052_42.0_Oct23_00.07.22.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 149.5 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.066 -0.731 IS units 1915.5 874.9 unbinned pixels; need stage -0.360 0.873 if reset Tilting to 44 Correcting tilt backlash -8.9 -5.3 unbinned pixels; 0.7 3.6 nm along two shift axes; 2.1 -2.9 nm on specimen axes Rate = 18.1773 A/sec -1.9 4.7 unbinned pixels; 1.8 0.1 nm along two shift axes; -1.6 -0.8 nm on specimen axes Rate = 8.83951 A/sec -2.6 1.2 unbinned pixels; 0.7 0.7 nm along two shift axes; -0.4 -0.9 nm on specimen axes Rate = 4.98709 A/sec Drift is low enough after shot 3 Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.09 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -3.493 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_053_44.0_Oct23_00.08.32.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 90.7 In SetExposureForMean 90, change by a factor of 0.9908 would require too small a change in exposure time Image shift -0.013 -0.785 IS units 2114.3 783.3 unbinned pixels; need stage -0.335 1.004 if reset Tilting to -64 -64.00 degrees 2.7 -0.7 unbinned pixels; 0.5 0.8 nm along two shift axes; 0.2 1.0 nm on specimen axes Rate = 4.86296 A/sec Drift is low enough after shot 1 Measured defocus = -4.11 microns changed by -0.14 to target drift = 0.13 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -0.399 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_054_-64.0_Oct23_00.09.35.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 38.0 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift 0.215 -1.374 IS units 3935.1 722.9 unbinned pixels; need stage -0.370 3.120 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash -13.7 1.1 unbinned pixels; 2.0 4.4 nm along two shift axes; 0.0 -4.8 nm on specimen axes Rate = 24.06912 A/sec -9.0 4.0 unbinned pixels; 2.4 2.5 nm along two shift axes; -1.1 -3.3 nm on specimen axes Rate = 17.20354 A/sec -6.9 2.3 unbinned pixels; 1.6 2.0 nm along two shift axes; -0.6 -2.5 nm on specimen axes Rate = 12.68982 A/sec -5.8 1.9 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 10.71342 A/sec -4.1 2.0 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.6 -1.5 nm on specimen axes Rate = 7.92961 A/sec Drift is low enough after shot 5 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.11 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -3.613 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_055_46.0_Oct23_00.11.03.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 166.6 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.036 -0.815 IS units 2173.5 873.6 unbinned pixels; need stage -0.367 1.066 if reset Tilting to 48 Correcting tilt backlash -10.2 -7.1 unbinned pixels; 1.2 4.2 nm along two shift axes; 2.8 -3.4 nm on specimen axes Rate = 21.98373 A/sec -2.4 6.5 unbinned pixels; 2.4 0.0 nm along two shift axes; -2.2 -1.1 nm on specimen axes Rate = 12.22436 A/sec -2.3 1.4 unbinned pixels; 0.7 0.6 nm along two shift axes; -0.4 -0.9 nm on specimen axes Rate = 4.76623 A/sec Drift is low enough after shot 3 Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.07 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -3.603 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_04_8_056_48.0_Oct23_00.12.13.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 84.7 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift 0.026 -0.885 IS units 2424.5 771.7 unbinned pixels; need stage -0.340 1.244 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -2.92 microns changed by -1.33 to target drift = 0.28 nm/sec Focus area changed from stored state, axis position -2.40 angle 0, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_05.st Running script 1 at item 10 (250.93, -45.76) 00:12:58 Refine ZLP: additional offset = 0.7 eV; total offset = 6.1 eV Item 10: Stage: 250.93 -45.76 -237 Label: 10 Note: Sec 8 - anchor_maps.map Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Target defocus is: -4.25 um Target defocus is: -4.50 um Target defocus = -4.5 Angle = -4.40, increment = 0.60, shift = -10.8 -3.7 pixels, -0.026 um Y Angle = -3.20, increment = 1.80, shift = -11.0 -5.1 pixels, -0.026 um Y Angle = 0.41, increment = 5.41, shift = -53.7 -11.1 pixels, -0.125 um Y Angle = 3.00, increment = 8.00, shift = -63.6 -13.5 pixels, -0.148 um Y Rough eucentricity: changing Z by 1.06 to -235.66, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = -33.3, 4.0 pixels, 0.018, -0.075 um, IS = -0.018, 0.075 Angle = 11.00, shift = -71.0, -9.9 pixels, -0.004, -0.164 um, IS = -0.013, 0.238 Angle = 4.00, shift = -62.4, -4.5 pixels, 0.006, -0.143 um, IS = -0.019, 0.381 Angle = -4.00, shift = -2.0, -19.9 pixels, -0.045, -0.010 um, IS = 0.026, 0.390 Angle = -11.00, shift = -1.4, -14.5 pixels, -0.033, -0.007 um, IS = 0.058, 0.397 Angle = -18.00, shift = 24.4, 5.5 pixels, 0.006, 0.057 um, IS = 0.052, 0.341 Angle = -24.00, shift = 7.0, 3.3 pixels, 0.006, 0.017 um, IS = 0.046, 0.324 General solution delZ 0.41, delY 2.29, yZero 0.37 solution with assumed YZero 0.39: delZ 0.41, delY 2.48 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.41 microns and lateral displacement of -2.29 microns Disparity in stage position after aligning to center of map frame: -0.20 -0.09 Aligning to target with image shift equivalent to stage shift: -0.21 -0.09 Disparity in stage position after aligning to map item itself: -0.20 -0.06 (align shift -0.01 0.00) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 250.94 -45.72 -236.05 -0.2 1.8 unbinned pixels; 0.6 0.1 nm along two shift axes; -0.6 -0.1 nm on specimen axes Rate = 3.12551 A/sec Drift is low enough after shot 1 Measured defocus = -3.03 microns changed by -1.47 to target drift = 0.06 nm/sec Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.529 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_000_-10.0_Oct23_00.20.51.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 78.3 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.232 -0.011 IS units -200.1 642.4 unbinned pixels; need stage -0.215 -0.093 if reset Tilting to -8 Correcting tilt backlash 5.8 1.1 unbinned pixels; 0.3 2.1 nm along two shift axes; -0.6 2.0 nm on specimen axes Rate = 10.37425 A/sec 1.5 1.6 unbinned pixels; 0.4 0.7 nm along two shift axes; -0.6 0.5 nm on specimen axes Rate = 3.87907 A/sec Drift is low enough after shot 2 Measured defocus = -4.37 microns changed by -0.13 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -1.662 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_001_-8.0_Oct23_00.21.54.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 86.5 In SetExposureForMean 90, change by a factor of 1.0402 would require too small a change in exposure time Image shift -0.274 -0.024 IS units -205.1 770.2 unbinned pixels; need stage -0.259 -0.098 if reset Tilting to -12 -12.00 degrees -6.7 5.0 unbinned pixels; 2.4 1.6 nm along two shift axes; -1.5 -2.5 nm on specimen axes Rate = 14.66192 A/sec -0.2 3.3 unbinned pixels; 1.1 0.3 nm along two shift axes; -1.2 -0.2 nm on specimen axes Rate = 5.84987 A/sec Drift is low enough after shot 2 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -1.479 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_002_-12.0_Oct23_00.22.56.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 85.6 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.332 0.074 IS units -526.4 832.9 unbinned pixels; need stage -0.270 -0.218 if reset Tilting to -14 -0.9 1.2 unbinned pixels; 0.5 0.2 nm along two shift axes; -0.4 -0.4 nm on specimen axes Rate = 2.70803 A/sec Drift is low enough after shot 1 Measured defocus = -4.61 microns changed by 0.11 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.357 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_003_-14.0_Oct23_00.23.52.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 92.6 In SetExposureForMean 90, change by a factor of 0.9720 would require too small a change in exposure time Image shift -0.375 0.156 IS units -793.5 873.0 unbinned pixels; need stage -0.276 -0.318 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 8.7 -1.9 unbinned pixels; 1.7 2.6 nm along two shift axes; 0.4 3.1 nm on specimen axes Rate = 15.61917 A/sec 7.8 0.4 unbinned pixels; 0.8 2.6 nm along two shift axes; -0.4 2.7 nm on specimen axes Rate = 13.78748 A/sec 3.6 0.1 unbinned pixels; 0.4 1.2 nm along two shift axes; -0.2 1.3 nm on specimen axes Rate = 6.36286 A/sec Drift is low enough after shot 3 Measured defocus = -4.39 microns changed by -0.11 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -1.770 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_004_-6.0_Oct23_00.25.03.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 94.8 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.261 -0.078 IS units -46.4 783.8 unbinned pixels; need stage -0.268 -0.042 if reset Tilting to -4 Correcting tilt backlash -5.7 -6.2 unbinned pixels; 1.4 2.6 nm along two shift axes; 2.3 -1.8 nm on specimen axes Rate = 14.81823 A/sec 0.7 3.7 unbinned pixels; 1.2 0.7 nm along two shift axes; -1.3 0.1 nm on specimen axes Rate = 6.71102 A/sec Drift is low enough after shot 2 Measured defocus = -4.39 microns changed by -0.11 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.870 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_005_-4.0_Oct23_00.26.07.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 87.0 In SetExposureForMean 90, change by a factor of 1.0344 would require too small a change in exposure time Image shift -0.223 -0.150 IS units 186.6 749.2 unbinned pixels; need stage -0.264 0.042 if reset Tilting to -16 -16.00 degrees -9.0 -4.9 unbinned pixels; 0.6 3.5 nm along two shift axes; 2.0 -3.0 nm on specimen axes Rate = 17.98228 A/sec -1.3 3.6 unbinned pixels; 1.3 0.0 nm along two shift axes; -1.2 -0.6 nm on specimen axes Rate = 6.71305 A/sec Drift is low enough after shot 2 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.307 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_006_-16.0_Oct23_00.27.11.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 84.0 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.352 0.084 IS units -573.7 879.6 unbinned pixels; need stage -0.285 -0.241 if reset Tilting to -18 4.7 -0.7 unbinned pixels; 0.8 1.5 nm along two shift axes; 0.1 1.7 nm on specimen axes Rate = 8.32661 A/sec 1.8 0.2 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.1 0.6 nm on specimen axes Rate = 3.26568 A/sec Drift is low enough after shot 2 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -1.221 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_007_-18.0_Oct23_00.28.13.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 90.7 In SetExposureForMean 90, change by a factor of 0.9922 would require too small a change in exposure time Image shift -0.352 0.083 IS units -573.2 880.0 unbinned pixels; need stage -0.285 -0.243 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 12.0 0.0 unbinned pixels; 1.4 4.0 nm along two shift axes; -0.4 4.2 nm on specimen axes Rate = 21.05926 A/sec 6.5 0.1 unbinned pixels; 0.7 2.2 nm along two shift axes; -0.2 2.3 nm on specimen axes Rate = 11.47727 A/sec 3.3 0.3 unbinned pixels; 0.3 1.1 nm along two shift axes; -0.2 1.2 nm on specimen axes Rate = 5.85862 A/sec Drift is low enough after shot 3 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.08 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -1.949 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_008_-2.0_Oct23_00.29.24.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 95.1 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.223 -0.172 IS units 246.7 769.5 unbinned pixels; need stage -0.272 0.062 if reset Tilting to 0 Correcting tilt backlash -7.9 -0.9 unbinned pixels; 0.6 2.7 nm along two shift axes; 0.6 -2.7 nm on specimen axes Rate = 13.91226 A/sec -1.6 1.2 unbinned pixels; 0.6 0.4 nm along two shift axes; -0.4 -0.6 nm on specimen axes Rate = 3.53961 A/sec Drift is low enough after shot 2 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.06 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -2.027 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_009_-0.0_Oct23_00.30.28.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 87.1 In SetExposureForMean 90, change by a factor of 1.0330 would require too small a change in exposure time Image shift -0.205 -0.196 IS units 330.7 744.5 unbinned pixels; need stage -0.266 0.093 if reset Tilting to -20 -20.00 degrees -6.2 -5.2 unbinned pixels; 1.0 2.7 nm along two shift axes; 2.0 -2.0 nm on specimen axes Rate = 14.21084 A/sec 0.3 2.8 unbinned pixels; 0.9 0.4 nm along two shift axes; -1.0 0.0 nm on specimen axes Rate = 4.99928 A/sec Drift is low enough after shot 2 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.194 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_010_-20.0_Oct23_00.31.33.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 81.9 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.324 0.007 IS units -338.0 874.1 unbinned pixels; need stage -0.290 -0.157 if reset Tilting to -22 6.0 -0.3 unbinned pixels; 0.8 1.9 nm along two shift axes; -0.1 2.1 nm on specimen axes Rate = 10.45059 A/sec 2.4 0.2 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.2 0.8 nm on specimen axes Rate = 4.15077 A/sec Drift is low enough after shot 2 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.106 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_011_-22.0_Oct23_00.32.34.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 88.2 In SetExposureForMean 90, change by a factor of 1.0208 would require too small a change in exposure time Image shift -0.328 0.017 IS units -368.2 875.8 unbinned pixels; need stage -0.290 -0.171 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 8.7 0.1 unbinned pixels; 1.0 2.9 nm along two shift axes; -0.3 3.0 nm on specimen axes Rate = 15.22706 A/sec 6.8 1.8 unbinned pixels; 0.2 2.5 nm along two shift axes; -0.8 2.3 nm on specimen axes Rate = 12.2891 A/sec 3.5 0.8 unbinned pixels; 0.2 1.3 nm along two shift axes; -0.4 1.2 nm on specimen axes Rate = 6.36237 A/sec Drift is low enough after shot 3 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.06 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -2.097 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_012_2.0_Oct23_00.33.45.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 94.8 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.235 -0.171 IS units 232.3 802.3 unbinned pixels; need stage -0.283 0.056 if reset Tilting to 4 Correcting tilt backlash -12.4 0.7 unbinned pixels; 1.7 4.0 nm along two shift axes; 0.1 -4.4 nm on specimen axes Rate = 21.86892 A/sec -7.7 1.7 unbinned pixels; 1.5 2.3 nm along two shift axes; -0.4 -2.8 nm on specimen axes Rate = 13.82545 A/sec -6.9 1.5 unbinned pixels; 1.3 2.1 nm along two shift axes; -0.3 -2.4 nm on specimen axes Rate = 12.30395 A/sec -5.5 1.1 unbinned pixels; 1.0 1.7 nm along two shift axes; -0.2 -2.0 nm on specimen axes Rate = 9.90376 A/sec -4.0 1.0 unbinned pixels; 0.8 1.2 nm along two shift axes; -0.2 -1.4 nm on specimen axes Rate = 7.30592 A/sec Drift is low enough after shot 5 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.10 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.10 nm/sec Defocus = -2.175 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_013_4.0_Oct23_00.35.06.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 86.7 In SetExposureForMean 90, change by a factor of 1.0379 would require too small a change in exposure time Image shift -0.226 -0.179 IS units 263.2 785.4 unbinned pixels; need stage -0.278 0.068 if reset Tilting to -24 -24.00 degrees -5.4 -1.8 unbinned pixels; 0.0 2.0 nm along two shift axes; 0.8 -1.8 nm on specimen axes Rate = 10.00669 A/sec 1.5 0.9 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.4 0.5 nm on specimen axes Rate = 3.03378 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.01 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -1.067 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_014_-24.0_Oct23_00.36.11.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 79.5 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.312 -0.037 IS units -206.2 885.9 unbinned pixels; need stage -0.298 -0.111 if reset Tilting to -26 5.3 0.1 unbinned pixels; 0.6 1.8 nm along two shift axes; -0.2 1.8 nm on specimen axes Rate = 9.23318 A/sec 2.5 0.2 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.1 0.9 nm on specimen axes Rate = 4.40541 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.06 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -0.993 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_015_-26.0_Oct23_00.37.13.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 85.1 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.318 -0.022 IS units -253.8 887.3 unbinned pixels; need stage -0.298 -0.132 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 8.5 1.1 unbinned pixels; 0.6 3.0 nm along two shift axes; -0.7 3.0 nm on specimen axes Rate = 15.1335 A/sec 4.4 0.1 unbinned pixels; 0.5 1.5 nm along two shift axes; -0.2 1.6 nm on specimen axes Rate = 7.8103 A/sec Drift is low enough after shot 2 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -2.229 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_016_6.0_Oct23_00.38.19.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 102.1 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.236 -0.192 IS units 288.3 827.0 unbinned pixels; need stage -0.293 0.076 if reset Tilting to 8 Correcting tilt backlash -17.0 -1.9 unbinned pixels; 1.3 5.8 nm along two shift axes; 1.2 -5.9 nm on specimen axes Rate = 29.99449 A/sec -9.1 4.9 unbinned pixels; 2.7 2.4 nm along two shift axes; -1.4 -3.3 nm on specimen axes Rate = 18.03799 A/sec -7.7 1.7 unbinned pixels; 1.5 2.3 nm along two shift axes; -0.4 -2.7 nm on specimen axes Rate = 13.80373 A/sec -5.5 1.6 unbinned pixels; 1.2 1.6 nm along two shift axes; -0.4 -2.0 nm on specimen axes Rate = 10.04503 A/sec -4.9 1.4 unbinned pixels; 1.0 1.4 nm along two shift axes; -0.3 -1.7 nm on specimen axes Rate = 8.87443 A/sec -3.3 0.8 unbinned pixels; 0.6 1.0 nm along two shift axes; -0.2 -1.2 nm on specimen axes Rate = 5.92028 A/sec Drift is low enough after shot 6 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.11 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.10 nm/sec Defocus = -2.303 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_017_8.0_Oct23_00.39.45.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 86.0 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.220 -0.215 IS units 365.8 804.6 unbinned pixels; need stage -0.288 0.104 if reset Tilting to -28 -28.00 degrees -2.1 -0.5 unbinned pixels; 0.1 0.8 nm along two shift axes; 0.2 -0.7 nm on specimen axes Rate = 3.859 A/sec Drift is low enough after shot 1 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.02 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.937 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_018_-28.0_Oct23_00.40.45.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 83.5 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.313 -0.060 IS units -145.9 908.8 unbinned pixels; need stage -0.308 -0.092 if reset Tilting to -30 3.6 0.3 unbinned pixels; 0.3 1.2 nm along two shift axes; -0.2 1.2 nm on specimen axes Rate = 6.26676 A/sec Drift is low enough after shot 1 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.04 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -0.860 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_019_-30.0_Oct23_00.41.44.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 88.4 In SetExposureForMean 90, change by a factor of 1.0178 would require too small a change in exposure time Image shift -0.306 -0.072 IS units -107.7 902.7 unbinned pixels; need stage -0.307 -0.078 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees 2.0 -0.4 unbinned pixels; 0.4 0.6 nm along two shift axes; 0.1 0.7 nm on specimen axes Rate = 3.5171 A/sec Drift is low enough after shot 1 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -2.371 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_020_10.0_Oct23_00.42.45.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 100.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.221 -0.260 IS units 486.2 847.6 unbinned pixels; need stage -0.307 0.147 if reset Tilting to 12 Correcting tilt backlash -14.9 -1.9 unbinned pixels; 1.1 5.2 nm along two shift axes; 1.1 -5.2 nm on specimen axes Rate = 26.46038 A/sec -7.6 4.5 unbinned pixels; 2.4 2.0 nm along two shift axes; -1.4 -2.8 nm on specimen axes Rate = 15.52505 A/sec -6.7 2.2 unbinned pixels; 1.5 1.9 nm along two shift axes; -0.6 -2.4 nm on specimen axes Rate = 12.2741 A/sec -5.6 2.5 unbinned pixels; 1.5 1.6 nm along two shift axes; -0.7 -2.0 nm on specimen axes Rate = 10.77263 A/sec -4.8 1.5 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.83188 A/sec -2.8 1.6 unbinned pixels; 0.8 0.7 nm along two shift axes; -0.5 -1.0 nm on specimen axes Rate = 5.56646 A/sec Drift is low enough after shot 6 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.11 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -2.437 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_021_12.0_Oct23_00.44.17.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 92.1 In SetExposureForMean 90, change by a factor of 0.9771 would require too small a change in exposure time Image shift -0.199 -0.294 IS units 601.7 822.4 unbinned pixels; need stage -0.302 0.190 if reset Tilting to -32 -32.00 degrees -1.9 0.2 unbinned pixels; 0.3 0.6 nm along two shift axes; -0.0 -0.7 nm on specimen axes Rate = 3.42814 A/sec Drift is low enough after shot 1 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.03 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.790 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_022_-32.0_Oct23_00.45.17.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 79.8 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.314 -0.091 IS units -61.2 941.3 unbinned pixels; need stage -0.322 -0.061 if reset Tilting to -34 4.0 -0.7 unbinned pixels; 0.7 1.2 nm along two shift axes; 0.1 1.4 nm on specimen axes Rate = 7.0766 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.06 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.718 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_023_-34.0_Oct23_00.46.14.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 90.9 In SetExposureForMean 90, change by a factor of 0.9912 would require too small a change in exposure time Image shift -0.306 -0.102 IS units -25.4 931.2 unbinned pixels; need stage -0.320 -0.047 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -3.3 0.1 unbinned pixels; 0.4 1.1 nm along two shift axes; 0.0 -1.2 nm on specimen axes Rate = 5.77024 A/sec Drift is low enough after shot 1 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -2.479 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_024_14.0_Oct23_00.47.16.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 106.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.193 -0.340 IS units 730.7 847.8 unbinned pixels; need stage -0.314 0.238 if reset Tilting to 16 Correcting tilt backlash -15.3 -0.4 unbinned pixels; 1.7 5.1 nm along two shift axes; 0.6 -5.4 nm on specimen axes Rate = 26.91272 A/sec -8.4 6.0 unbinned pixels; 3.0 2.1 nm along two shift axes; -1.9 -3.1 nm on specimen axes Rate = 18.15486 A/sec -6.9 2.6 unbinned pixels; 1.7 2.0 nm along two shift axes; -0.7 -2.5 nm on specimen axes Rate = 12.99302 A/sec -4.8 2.5 unbinned pixels; 1.4 1.3 nm along two shift axes; -0.7 -1.8 nm on specimen axes Rate = 9.53196 A/sec -3.8 1.5 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.4 -1.4 nm on specimen axes Rate = 7.14485 A/sec Drift is low enough after shot 5 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.10 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -2.538 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_025_16.0_Oct23_00.48.38.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 90.5 In SetExposureForMean 90, change by a factor of 0.9950 would require too small a change in exposure time Image shift -0.170 -0.381 IS units 864.1 824.6 unbinned pixels; need stage -0.310 0.290 if reset Tilting to -36 -36.00 degrees -2.9 -1.4 unbinned pixels; 0.1 1.1 nm along two shift axes; 0.6 -1.0 nm on specimen axes Rate = 5.67473 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -0.664 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_026_-36.0_Oct23_00.49.38.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 75.6 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.297 -0.158 IS units 136.4 959.8 unbinned pixels; need stage -0.334 0.021 if reset Tilting to -38 5.2 -0.4 unbinned pixels; 0.7 1.7 nm along two shift axes; -0.0 1.8 nm on specimen axes Rate = 9.17335 A/sec 2.1 0.4 unbinned pixels; 0.1 0.7 nm along two shift axes; -0.2 0.7 nm on specimen axes Rate = 3.74536 A/sec Drift is low enough after shot 2 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.614 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_027_-38.0_Oct23_00.50.40.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 85.3 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.289 -0.171 IS units 179.0 948.6 unbinned pixels; need stage -0.332 0.041 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -13.3 2.2 unbinned pixels; 2.3 4.2 nm along two shift axes; -0.3 -4.7 nm on specimen axes Rate = 23.69194 A/sec -5.7 4.2 unbinned pixels; 2.1 1.4 nm along two shift axes; -1.3 -2.1 nm on specimen axes Rate = 12.35808 A/sec -2.9 1.9 unbinned pixels; 1.0 0.7 nm along two shift axes; -0.6 -1.1 nm on specimen axes Rate = 6.06663 A/sec Drift is low enough after shot 3 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.10 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.620 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_028_18.0_Oct23_00.51.55.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 111.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.168 -0.401 IS units 921.3 840.4 unbinned pixels; need stage -0.318 0.314 if reset Tilting to 20 Correcting tilt backlash 2.5 -3.1 unbinned pixels; 1.3 0.5 nm along two shift axes; 1.0 1.0 nm on specimen axes Rate = 7.04311 A/sec Drift is low enough after shot 1 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.10 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -2.651 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_029_20.0_Oct23_00.52.54.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 88.2 In SetExposureForMean 90, change by a factor of 1.0222 would require too small a change in exposure time Image shift -0.127 -0.495 IS units 1215.4 816.9 unbinned pixels; need stage -0.319 0.429 if reset Tilting to -40 -40.00 degrees -0.9 -4.7 unbinned pixels; 1.5 0.9 nm along two shift axes; 1.7 -0.2 nm on specimen axes Rate = 8.49217 A/sec -1.0 4.9 unbinned pixels; 1.7 0.3 nm along two shift axes; -1.7 -0.5 nm on specimen axes Rate = 8.73313 A/sec 0.4 1.3 unbinned pixels; 0.4 0.3 nm along two shift axes; -0.5 0.1 nm on specimen axes Rate = 2.43169 A/sec Drift is low enough after shot 3 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = -0.562 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_030_-40.0_Oct23_00.54.12.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 70.6 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.258 -0.274 IS units 488.7 963.5 unbinned pixels; need stage -0.347 0.185 if reset Tilting to -42 0.1 -4.9 unbinned pixels; 1.6 0.6 nm along two shift axes; 1.7 0.2 nm on specimen axes Rate = 8.58596 A/sec 2.0 2.3 unbinned pixels; 0.5 0.9 nm along two shift axes; -0.9 0.6 nm on specimen axes Rate = 5.35564 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.03 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.525 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_031_-42.0_Oct23_00.55.13.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 84.8 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.230 -0.323 IS units 647.2 934.4 unbinned pixels; need stage -0.341 0.267 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -13.3 2.9 unbinned pixels; 2.5 4.1 nm along two shift axes; -0.6 -4.8 nm on specimen axes Rate = 23.92597 A/sec -4.1 5.3 unbinned pixels; 2.2 0.7 nm along two shift axes; -1.7 -1.6 nm on specimen axes Rate = 11.68849 A/sec -2.7 0.7 unbinned pixels; 0.5 0.8 nm along two shift axes; -0.2 -1.0 nm on specimen axes Rate = 4.84156 A/sec Drift is low enough after shot 3 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.756 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_032_22.0_Oct23_00.56.28.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 115.3 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.159 -0.430 IS units 1008.3 843.6 unbinned pixels; need stage -0.321 0.355 if reset Tilting to 24 Correcting tilt backlash 0.7 0.9 unbinned pixels; 0.2 0.4 nm along two shift axes; -0.4 0.2 nm on specimen axes Rate = 2.1172 A/sec Drift is low enough after shot 1 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.09 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.791 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_033_24.0_Oct23_00.57.32.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 92.0 In SetExposureForMean 90, change by a factor of 0.9797 would require too small a change in exposure time Image shift -0.138 -0.501 IS units 1220.9 851.6 unbinned pixels; need stage -0.331 0.442 if reset Tilting to -44 -44.00 degrees -0.0 -5.7 unbinned pixels; 1.9 0.7 nm along two shift axes; 2.0 0.2 nm on specimen axes Rate = 10.13651 A/sec 1.1 2.9 unbinned pixels; 0.8 0.7 nm along two shift axes; -1.0 0.3 nm on specimen axes Rate = 5.43114 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.02 nm/sec Defocus = -0.474 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_034_-44.0_Oct23_00.58.39.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 70.3 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift -0.184 -0.442 IS units 1016.7 922.3 unbinned pixels; need stage -0.349 0.458 if reset Tilting to -46 2.5 -5.2 unbinned pixels; 2.0 0.2 nm along two shift axes; 1.8 1.0 nm on specimen axes Rate = 10.23138 A/sec 3.5 1.7 unbinned pixels; 0.1 1.4 nm along two shift axes; -0.7 1.2 nm on specimen axes Rate = 6.92469 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.03 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.02 nm/sec Defocus = -0.416 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_035_-46.0_Oct23_00.59.41.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 87.6 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift -0.155 -0.500 IS units 1203.4 897.5 unbinned pixels; need stage -0.346 0.571 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -21.0 8.0 unbinned pixels; 5.1 6.0 nm along two shift axes; -2.2 -7.6 nm on specimen axes Rate = 39.31666 A/sec -9.1 3.6 unbinned pixels; 2.3 2.6 nm along two shift axes; -1.0 -3.3 nm on specimen axes Rate = 17.10383 A/sec -7.0 2.5 unbinned pixels; 1.6 2.0 nm along two shift axes; -0.7 -2.5 nm on specimen axes Rate = 12.98267 A/sec -4.5 1.6 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.34297 A/sec -3.9 1.2 unbinned pixels; 0.8 1.2 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 7.1667 A/sec Drift is low enough after shot 5 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.09 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -2.907 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_036_26.0_Oct23_01.01.08.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 125.0 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.150 -0.482 IS units 1158.5 868.4 unbinned pixels; need stage -0.334 0.424 if reset Tilting to 28 Correcting tilt backlash 6.9 -3.4 unbinned pixels; 1.9 1.9 nm along two shift axes; 1.0 2.5 nm on specimen axes Rate = 13.45133 A/sec -0.5 7.5 unbinned pixels; 2.5 0.7 nm along two shift axes; -2.6 -0.4 nm on specimen axes Rate = 13.21946 A/sec -0.7 3.0 unbinned pixels; 1.1 0.1 nm along two shift axes; -1.0 -0.4 nm on specimen axes Rate = 5.4279 A/sec Drift is low enough after shot 3 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.929 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_037_28.0_Oct23_01.02.23.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 88.3 In SetExposureForMean 90, change by a factor of 1.0226 would require too small a change in exposure time Image shift -0.078 -0.640 IS units 1656.0 821.1 unbinned pixels; need stage -0.334 0.633 if reset Tilting to -48 -48.00 degrees -0.4 -3.1 unbinned pixels; 1.0 0.5 nm along two shift axes; 1.1 -0.1 nm on specimen axes Rate = 5.54667 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.350 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_038_-48.0_Oct23_01.03.26.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 63.6 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift -0.121 -0.588 IS units 1472.4 889.0 unbinned pixels; need stage -0.351 0.735 if reset Tilting to -50 3.7 -5.0 unbinned pixels; 2.1 0.6 nm along two shift axes; 1.6 1.4 nm on specimen axes Rate = 10.89077 A/sec 3.3 2.3 unbinned pixels; 0.4 1.4 nm along two shift axes; -0.9 1.1 nm on specimen axes Rate = 7.08522 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -0.323 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_039_-50.0_Oct23_01.04.27.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 87.6 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift -0.096 -0.634 IS units 1621.8 865.0 unbinned pixels; need stage -0.348 0.848 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -15.2 6.9 unbinned pixels; 4.1 4.2 nm along two shift axes; -2.0 -5.5 nm on specimen axes Rate = 29.18381 A/sec -10.5 1.8 unbinned pixels; 1.8 3.3 nm along two shift axes; -0.3 -3.7 nm on specimen axes Rate = 18.71358 A/sec -4.9 4.2 unbinned pixels; 2.0 1.1 nm along two shift axes; -1.3 -1.9 nm on specimen axes Rate = 11.35317 A/sec -3.8 1.4 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.4 -1.4 nm on specimen axes Rate = 7.11938 A/sec Drift is low enough after shot 4 Measured defocus = -4.35 microns changed by -0.15 to target drift = 0.09 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -3.086 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_040_30.0_Oct23_01.05.49.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 132.5 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.094 -0.613 IS units 1567.6 839.2 unbinned pixels; need stage -0.337 0.609 if reset Tilting to 32 Correcting tilt backlash -8.9 3.9 unbinned pixels; 2.3 2.5 nm along two shift axes; -1.1 -3.2 nm on specimen axes Rate = 17.00036 A/sec -4.4 6.9 unbinned pixels; 2.8 0.6 nm along two shift axes; -2.3 -1.7 nm on specimen axes Rate = 14.38876 A/sec -1.7 3.5 unbinned pixels; 1.4 0.1 nm along two shift axes; -1.2 -0.7 nm on specimen axes Rate = 6.8472 A/sec Drift is low enough after shot 3 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.09 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -3.051 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_041_32.0_Oct23_01.07.01.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 90.4 In SetExposureForMean 90, change by a factor of 0.9993 would require too small a change in exposure time Image shift -0.099 -0.773 IS units 1996.6 1004.5 unbinned pixels; need stage -0.407 0.794 if reset Tilting to -52 -52.00 degrees 2.5 -5.6 unbinned pixels; 2.1 0.1 nm along two shift axes; 1.9 1.0 nm on specimen axes Rate = 10.74766 A/sec 1.6 2.6 unbinned pixels; 0.7 0.8 nm along two shift axes; -1.0 0.5 nm on specimen axes Rate = 5.37265 A/sec Drift is low enough after shot 2 Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.03 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.356 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_042_-52.0_Oct23_01.08.10.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 60.5 In SetExposureForMean 90, exposure time changed to 3.3600 Image shift -0.058 -0.758 IS units 1998.2 878.6 unbinned pixels; need stage -0.364 1.101 if reset Tilting to -54 -0.8 -3.3 unbinned pixels; 1.0 0.7 nm along two shift axes; 1.2 -0.2 nm on specimen axes Rate = 6.0572 A/sec Drift is low enough after shot 1 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = -0.321 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_043_-54.0_Oct23_01.09.06.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 83.9 In SetExposureForMean 90, exposure time changed to 3.6800 Image shift -0.033 -0.793 IS units 2114.7 845.3 unbinned pixels; need stage -0.356 1.225 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -13.7 -1.7 unbinned pixels; 1.0 4.7 nm along two shift axes; 1.0 -4.7 nm on specimen axes Rate = 24.18197 A/sec -12.4 6.4 unbinned pixels; 3.6 3.3 nm along two shift axes; -1.9 -4.5 nm on specimen axes Rate = 24.41701 A/sec -4.7 3.8 unbinned pixels; 1.8 1.1 nm along two shift axes; -1.2 -1.8 nm on specimen axes Rate = 10.64644 A/sec -4.1 2.5 unbinned pixels; 1.3 1.1 nm along two shift axes; -0.8 -1.5 nm on specimen axes Rate = 8.49678 A/sec -3.9 1.3 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 7.20113 A/sec Drift is low enough after shot 5 Measured defocus = -4.34 microns changed by -0.16 to target drift = 0.10 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -3.217 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_044_34.0_Oct23_01.10.35.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 144.0 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.033 -0.743 IS units 1981.3 796.1 unbinned pixels; need stage -0.335 0.814 if reset Tilting to 36 Correcting tilt backlash -9.0 -0.2 unbinned pixels; 1.0 3.0 nm along two shift axes; 0.4 -3.1 nm on specimen axes Rate = 15.74243 A/sec -4.1 6.2 unbinned pixels; 2.5 0.6 nm along two shift axes; -2.0 -1.6 nm on specimen axes Rate = 12.98591 A/sec -1.9 2.2 unbinned pixels; 1.0 0.4 nm along two shift axes; -0.7 -0.7 nm on specimen axes Rate = 5.13608 A/sec Drift is low enough after shot 3 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.09 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -3.240 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_045_36.0_Oct23_01.11.46.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 84.8 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift 0.016 -0.843 IS units 2299.1 759.5 unbinned pixels; need stage -0.332 0.974 if reset Tilting to -56 -56.00 degrees 0.1 -3.5 unbinned pixels; 1.2 0.4 nm along two shift axes; 1.2 0.2 nm on specimen axes Rate = 6.19392 A/sec Drift is low enough after shot 1 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.05 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = -0.258 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_046_-56.0_Oct23_01.12.49.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 55.3 In SetExposureForMean 90, exposure time changed to 4.0000 Image shift -0.013 -0.860 IS units 2316.6 853.9 unbinned pixels; need stage -0.365 1.415 if reset Tilting to -58 -1.4 -2.4 unbinned pixels; 0.6 0.8 nm along two shift axes; 0.9 -0.4 nm on specimen axes Rate = 5.01843 A/sec Drift is low enough after shot 1 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.263 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_047_-58.0_Oct23_01.13.46.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 83.8 In SetExposureForMean 90, exposure time changed to 4.3200 Image shift 0.005 -0.892 IS units 2421.2 835.6 unbinned pixels; need stage -0.362 1.564 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -17.2 5.2 unbinned pixels; 3.7 5.1 nm along two shift axes; -1.3 -6.2 nm on specimen axes Rate = 31.46697 A/sec -12.8 2.6 unbinned pixels; 2.4 3.9 nm along two shift axes; -0.5 -4.6 nm on specimen axes Rate = 22.97295 A/sec -6.5 2.9 unbinned pixels; 1.7 1.8 nm along two shift axes; -0.8 -2.4 nm on specimen axes Rate = 12.51288 A/sec -6.1 2.3 unbinned pixels; 1.5 1.8 nm along two shift axes; -0.6 -2.2 nm on specimen axes Rate = 11.48987 A/sec -5.3 1.7 unbinned pixels; 1.2 1.6 nm along two shift axes; -0.4 -1.9 nm on specimen axes Rate = 9.71289 A/sec -3.6 0.8 unbinned pixels; 0.7 1.1 nm along two shift axes; -0.2 -1.3 nm on specimen axes Rate = 6.41331 A/sec Drift is low enough after shot 6 Measured defocus = -4.35 microns changed by -0.15 to target drift = 0.12 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -3.393 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_048_38.0_Oct23_01.15.21.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 157.2 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.009 -0.810 IS units 2184.5 795.0 unbinned pixels; need stage -0.341 0.947 if reset Tilting to 40 Correcting tilt backlash -11.8 -2.8 unbinned pixels; 0.5 4.2 nm along two shift axes; 1.3 -4.1 nm on specimen axes Rate = 21.32715 A/sec -1.8 4.8 unbinned pixels; 1.8 0.0 nm along two shift axes; -1.6 -0.8 nm on specimen axes Rate = 9.03812 A/sec -2.2 1.8 unbinned pixels; 0.9 0.5 nm along two shift axes; -0.6 -0.8 nm on specimen axes Rate = 5.04907 A/sec Drift is low enough after shot 3 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.06 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -3.359 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_049_40.0_Oct23_01.16.33.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 89.4 In SetExposureForMean 90, change by a factor of 1.0144 would require too small a change in exposure time Image shift 0.047 -0.883 IS units 2440.1 711.9 unbinned pixels; need stage -0.320 1.096 if reset Tilting to -60 -60.00 degrees 1.8 -3.7 unbinned pixels; 1.5 0.1 nm along two shift axes; 1.3 0.7 nm on specimen axes Rate = 7.2953 A/sec Drift is low enough after shot 1 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.05 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.01 nm/sec Defocus = -0.220 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_050_-60.0_Oct23_01.17.37.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 48.3 In SetExposureForMean 90, exposure time changed to 4.8000 Image shift 0.042 -0.977 IS units 2688.7 816.7 unbinned pixels; need stage -0.364 1.848 if reset Tilting to -62 -0.6 -3.4 unbinned pixels; 1.1 0.6 nm along two shift axes; 1.2 -0.1 nm on specimen axes Rate = 6.16519 A/sec Drift is low enough after shot 1 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.04 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.238 um Dewars are NOT busy filling 30 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_051_-62.0_Oct23_01.18.34.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 79.8 In SetExposureForMean 90, exposure time changed to 5.4400 Image shift 0.077 -1.053 IS units 2929.5 792.6 unbinned pixels; need stage -0.363 2.152 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -17.5 4.4 unbinned pixels; 3.5 5.3 nm along two shift axes; -1.0 -6.3 nm on specimen axes Rate = 31.58892 A/sec -11.5 3.2 unbinned pixels; 2.4 3.4 nm along two shift axes; -0.8 -4.1 nm on specimen axes Rate = 20.97078 A/sec -8.0 2.6 unbinned pixels; 1.8 2.3 nm along two shift axes; -0.7 -2.9 nm on specimen axes Rate = 14.73793 A/sec -7.2 1.8 unbinned pixels; 1.4 2.2 nm along two shift axes; -0.4 -2.6 nm on specimen axes Rate = 13.06896 A/sec -5.6 1.4 unbinned pixels; 1.1 1.7 nm along two shift axes; -0.3 -2.0 nm on specimen axes Rate = 10.13264 A/sec -4.6 1.9 unbinned pixels; 1.2 1.3 nm along two shift axes; -0.5 -1.7 nm on specimen axes Rate = 8.70429 A/sec -4.3 1.2 unbinned pixels; 0.9 1.3 nm along two shift axes; -0.3 -1.5 nm on specimen axes Rate = 7.83091 A/sec Drift is low enough after shot 7 Measured defocus = -4.39 microns changed by -0.11 to target drift = 0.12 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -3.477 um Dewars are NOT busy filling 34 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_052_42.0_Oct23_01.20.16.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 181.1 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift 0.029 -0.878 IS units 2406.9 757.5 unbinned pixels; need stage -0.335 1.112 if reset Tilting to 44 Correcting tilt backlash -4.8 -6.3 unbinned pixels; 1.5 2.4 nm along two shift axes; 2.4 -1.5 nm on specimen axes Rate = 14.05003 A/sec -2.1 2.7 unbinned pixels; 1.1 0.4 nm along two shift axes; -0.9 -0.8 nm on specimen axes Rate = 5.93429 A/sec Drift is low enough after shot 2 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -3.429 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_053_44.0_Oct23_01.21.23.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 86.1 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift 0.083 -0.981 IS units 2739.9 707.0 unbinned pixels; need stage -0.328 1.315 if reset Tilting to -64 -64.00 degrees Trimming A by 35 35, reference by 20 20 2.6 -5.7 unbinned pixels; 2.2 0.2 nm along two shift axes; 1.9 1.1 nm on specimen axes Rate = 11.03995 A/sec 1.6 2.6 unbinned pixels; 0.7 0.9 nm along two shift axes; -1.0 0.5 nm on specimen axes Rate = 5.46897 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.174 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_054_-64.0_Oct23_01.22.33.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 41.7 In SetExposureForMean 90, exposure time changed to 6.2400 Image shift 0.127 -1.136 IS units 3202.8 736.4 unbinned pixels; need stage -0.352 2.529 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash -26.0 -2.1 unbinned pixels; 2.3 8.9 nm along two shift axes; 1.5 -9.0 nm on specimen axes Rate = 45.77144 A/sec -10.8 5.9 unbinned pixels; 3.2 2.9 nm along two shift axes; -1.7 -4.0 nm on specimen axes Rate = 21.613 A/sec -8.1 3.0 unbinned pixels; 1.9 2.3 nm along two shift axes; -0.8 -2.9 nm on specimen axes Rate = 15.19476 A/sec -7.4 1.8 unbinned pixels; 1.5 2.2 nm along two shift axes; -0.4 -2.6 nm on specimen axes Rate = 13.34984 A/sec -5.4 2.0 unbinned pixels; 1.3 1.6 nm along two shift axes; -0.5 -2.0 nm on specimen axes Rate = 10.13947 A/sec -4.9 1.5 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.4 -1.8 nm on specimen axes Rate = 9.06089 A/sec -3.1 0.9 unbinned pixels; 0.7 0.9 nm along two shift axes; -0.2 -1.1 nm on specimen axes Rate = 5.61315 A/sec Drift is low enough after shot 7 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -3.504 um Dewars are NOT busy filling 39 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_055_46.0_Oct23_01.24.11.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 186.3 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift 0.094 -1.034 IS units 2892.4 728.3 unbinned pixels; need stage -0.340 1.438 if reset Tilting to 48 Correcting tilt backlash -5.8 -0.8 unbinned pixels; 0.4 2.0 nm along two shift axes; 0.5 -2.0 nm on specimen axes Rate = 10.33569 A/sec -4.4 2.5 unbinned pixels; 1.4 1.1 nm along two shift axes; -0.8 -1.6 nm on specimen axes Rate = 8.86079 A/sec -2.2 1.8 unbinned pixels; 0.8 0.5 nm along two shift axes; -0.5 -0.8 nm on specimen axes Rate = 4.93323 A/sec Drift is low enough after shot 3 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -3.433 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_05_10_056_48.0_Oct23_01.25.25.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 85.5 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift 0.134 -1.054 IS units 2986.5 638.4 unbinned pixels; need stage -0.312 1.547 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -2.99 microns changed by -1.51 to target drift = 0.43 nm/sec Focus area changed from stored state, axis position -2.40 angle 0, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_06.st Running script 1 at item 12 (248.81, -46.51) 01:26:15 Refine ZLP: additional offset = 0.2 eV; total offset = 6.4 eV Item 12: Stage: 248.81 -46.51 -237 Label: 12 Note: Sec 10 - anchor_maps.map Target defocus is: -4.50 um Target defocus is: -4.75 um Target defocus = -4.75 Trimming A by 20 80, reference by 20 80 Angle = -4.40, increment = 0.60, shift = -7.2 -3.3 pixels, -0.017 um Y Trimming A by 20 80, reference by 20 80 Angle = -3.20, increment = 1.80, shift = -10.2 -7.4 pixels, -0.025 um Y Trimming A by 20 80, reference by 20 80 Angle = 0.41, increment = 5.41, shift = 0.8 -8.9 pixels, -0.000 um Y Trimming A by 20 80, reference by 20 80 Angle = 3.00, increment = 8.00, shift = 16.7 -10.0 pixels, 0.035 um Y Rough eucentricity: changing Z by -0.25 to -236.98, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 20 85, reference by 20 85 Angle = 18.00, shift = 4.9, 13.1 pixels, 0.028, 0.014 um, IS = -0.028, -0.014 Trimming A by 20 85, reference by 20 85 Angle = 11.00, shift = 29.0, 7.1 pixels, 0.009, 0.068 um, IS = -0.037, -0.082 Trimming A by 20 80, reference by 20 85 Angle = 4.00, shift = -15.5, 4.2 pixels, 0.013, -0.034 um, IS = -0.051, -0.048 Trimming A by 20 20 20 60, reference by 20 20 20 70 Angle = -4.00, shift = -15.6, -23.7 pixels, -0.050, -0.042 um, IS = -0.001, -0.007 Trimming A by 20 80, reference by 21 80 Angle = -11.00, shift = 65.2, 3.7 pixels, -0.008, 0.149 um, IS = 0.008, -0.155 Trimming A by 20 85, reference by 20 80 Angle = -18.00, shift = 96.1, 19.3 pixels, 0.019, 0.223 um, IS = -0.012, -0.379 Trimming A by 20 85, reference by 20 85 Angle = -24.00, shift = 88.1, 18.3 pixels, 0.019, 0.205 um, IS = -0.031, -0.583 General solution delZ -0.60, delY 3.03, yZero -0.04 solution with assumed YZero -0.03: delZ -0.60, delY 3.24 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.60 microns and lateral displacement of -3.03 microns Trimming A by 21 20 20 85, reference by 21 20 20 100 Disparity in stage position after aligning to center of map frame: -0.20 -0.15 Aligning to target with image shift equivalent to stage shift: -0.20 -0.17 Disparity in stage position after aligning to map item itself: -0.20 -0.13 (align shift -0.01 0.00) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 248.83 -46.47 -236.36 -2.0 1.8 unbinned pixels; 0.8 0.4 nm along two shift axes; -0.6 -0.7 nm on specimen axes Rate = 4.66502 A/sec Drift is low enough after shot 1 Measured defocus = -3.09 microns changed by -1.66 to target drift = 0.06 nm/sec Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.725 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_000_-10.0_Oct23_01.30.01.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 82.3 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.262 0.054 IS units -405.3 660.5 unbinned pixels; need stage -0.215 -0.167 if reset Tilting to -8 Correcting tilt backlash 7.8 0.2 unbinned pixels; 0.8 2.6 nm along two shift axes; -0.3 2.7 nm on specimen axes Rate = 13.72694 A/sec 2.7 1.3 unbinned pixels; 0.1 1.0 nm along two shift axes; -0.5 0.9 nm on specimen axes Rate = 5.22466 A/sec Drift is low enough after shot 2 Measured defocus = -4.64 microns changed by -0.11 to target drift = 0.05 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -1.843 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_001_-8.0_Oct23_01.31.04.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 91.2 In SetExposureForMean 90, change by a factor of 0.9870 would require too small a change in exposure time Image shift -0.314 0.054 IS units -454.4 802.8 unbinned pixels; need stage -0.262 -0.189 if reset Tilting to -12 -12.00 degrees -10.2 4.2 unbinned pixels; 2.6 2.9 nm along two shift axes; -1.2 -3.7 nm on specimen axes Rate = 19.33834 A/sec -3.5 2.9 unbinned pixels; 1.4 0.8 nm along two shift axes; -0.9 -1.3 nm on specimen axes Rate = 7.99462 A/sec Drift is low enough after shot 2 Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.09 nm/sec Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.06 nm/sec Defocus = -1.682 um Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_002_-12.0_Oct23_01.40.14.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 89.7 In SetExposureForMean 90, change by a factor of 1.0036 would require too small a change in exposure time Image shift -0.357 0.142 IS units -735.6 836.8 unbinned pixels; need stage -0.265 -0.294 if reset Tilting to -14 -0.0 1.5 unbinned pixels; 0.5 0.2 nm along two shift axes; -0.5 -0.1 nm on specimen axes Rate = 2.71761 A/sec Drift is low enough after shot 1 Measured defocus = -4.89 microns changed by 0.14 to target drift = 0.03 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -1.539 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_003_-14.0_Oct23_01.41.15.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 89.4 In SetExposureForMean 90, change by a factor of 1.0077 would require too small a change in exposure time Image shift -0.383 0.180 IS units -865.4 870.4 unbinned pixels; need stage -0.273 -0.344 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 4.0 -3.2 unbinned pixels; 1.5 0.9 nm along two shift axes; 1.0 1.5 nm on specimen axes Rate = 8.9612 A/sec 6.8 1.4 unbinned pixels; 0.3 2.4 nm along two shift axes; -0.7 2.3 nm on specimen axes Rate = 12.1866 A/sec 2.1 0.9 unbinned pixels; 0.0 0.8 nm along two shift axes; -0.4 0.7 nm on specimen axes Rate = 4.06045 A/sec Drift is low enough after shot 3 Measured defocus = -4.66 microns changed by -0.09 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.920 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_004_-6.0_Oct23_01.42.25.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 91.2 In SetExposureForMean 90, change by a factor of 0.9865 would require too small a change in exposure time Image shift -0.299 0.009 IS units -320.1 805.9 unbinned pixels; need stage -0.267 -0.140 if reset Tilting to -4 Correcting tilt backlash -6.9 -5.2 unbinned pixels; 0.9 2.9 nm along two shift axes; 2.0 -2.3 nm on specimen axes Rate = 15.23417 A/sec -0.9 4.5 unbinned pixels; 1.6 0.3 nm along two shift axes; -1.6 -0.4 nm on specimen axes Rate = 8.14792 A/sec -1.7 1.4 unbinned pixels; 0.7 0.4 nm along two shift axes; -0.4 -0.6 nm on specimen axes Rate = 3.81008 A/sec Drift is low enough after shot 3 Measured defocus = -4.66 microns changed by -0.09 to target drift = 0.04 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -2.008 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_005_-4.0_Oct23_01.43.34.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 91.5 In SetExposureForMean 90, change by a factor of 0.9840 would require too small a change in exposure time Image shift -0.272 -0.047 IS units -139.7 786.5 unbinned pixels; need stage -0.266 -0.075 if reset Tilting to -16 -16.00 degrees -10.0 -4.6 unbinned pixels; 0.4 3.9 nm along two shift axes; 1.9 -3.4 nm on specimen axes Rate = 19.39282 A/sec -2.3 4.0 unbinned pixels; 1.6 0.3 nm along two shift axes; -1.3 -0.9 nm on specimen axes Rate = 8.03202 A/sec -2.4 1.3 unbinned pixels; 0.7 0.6 nm along two shift axes; -0.4 -0.9 nm on specimen axes Rate = 4.70164 A/sec Drift is low enough after shot 3 Measured defocus = -4.83 microns changed by 0.08 to target drift = 0.06 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -1.454 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_006_-16.0_Oct23_01.44.44.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 88.8 In SetExposureForMean 90, change by a factor of 1.0144 would require too small a change in exposure time Image shift -0.364 0.104 IS units -640.4 892.9 unbinned pixels; need stage -0.288 -0.266 if reset Tilting to -18 -0.7 -1.1 unbinned pixels; 0.3 0.4 nm along two shift axes; 0.4 -0.2 nm on specimen axes Rate = 2.2458 A/sec Drift is low enough after shot 1 Measured defocus = -4.86 microns changed by 0.11 to target drift = 0.04 nm/sec Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -1.334 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_007_-18.0_Oct23_01.45.40.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 87.8 In SetExposureForMean 90, change by a factor of 1.0256 would require too small a change in exposure time Image shift -0.353 0.078 IS units -558.3 886.9 unbinned pixels; need stage -0.288 -0.238 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 6.3 -1.2 unbinned pixels; 1.1 2.0 nm along two shift axes; 0.2 2.3 nm on specimen axes Rate = 11.32006 A/sec 6.8 1.0 unbinned pixels; 0.5 2.4 nm along two shift axes; -0.6 2.3 nm on specimen axes Rate = 12.00692 A/sec 3.1 1.5 unbinned pixels; 0.1 1.2 nm along two shift axes; -0.6 1.0 nm on specimen axes Rate = 6.08192 A/sec Drift is low enough after shot 3 Measured defocus = -4.71 microns changed by -0.04 to target drift = 0.05 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -2.044 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_008_-2.0_Oct23_01.46.51.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 91.4 In SetExposureForMean 90, change by a factor of 0.9846 would require too small a change in exposure time Image shift -0.258 -0.113 IS units 52.3 809.2 unbinned pixels; need stage -0.280 -0.008 if reset Tilting to 0 Correcting tilt backlash -9.4 -2.6 unbinned pixels; 0.2 3.4 nm along two shift axes; 1.2 -3.2 nm on specimen axes Rate = 17.09614 A/sec -3.4 3.4 unbinned pixels; 1.5 0.7 nm along two shift axes; -1.1 -1.3 nm on specimen axes Rate = 8.43866 A/sec -3.8 1.9 unbinned pixels; 1.1 1.0 nm along two shift axes; -0.6 -1.4 nm on specimen axes Rate = 7.42008 A/sec Drift is low enough after shot 3 Measured defocus = -4.68 microns changed by -0.07 to target drift = 0.08 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -2.121 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_009_-0.0_Oct23_01.48.00.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 91.3 In SetExposureForMean 90, change by a factor of 0.9861 would require too small a change in exposure time Image shift -0.237 -0.165 IS units 213.5 802.0 unbinned pixels; need stage -0.283 0.049 if reset Tilting to -20 -20.00 degrees -5.9 -5.5 unbinned pixels; 1.1 2.6 nm along two shift axes; 2.1 -1.9 nm on specimen axes Rate = 14.34116 A/sec -1.9 3.1 unbinned pixels; 1.3 0.3 nm along two shift axes; -1.0 -0.8 nm on specimen axes Rate = 6.45083 A/sec Drift is low enough after shot 2 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.04 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -1.263 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_010_-20.0_Oct23_01.49.04.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 86.9 In SetExposureForMean 90, change by a factor of 1.0367 would require too small a change in exposure time Image shift -0.314 -0.048 IS units -181.0 901.4 unbinned pixels; need stage -0.305 -0.099 if reset Tilting to -22 2.1 0.2 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.1 0.7 nm on specimen axes Rate = 3.67773 A/sec Drift is low enough after shot 1 Measured defocus = -4.81 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -1.183 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_011_-22.0_Oct23_01.50.05.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 86.0 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.307 -0.062 IS units -135.8 895.2 unbinned pixels; need stage -0.304 -0.083 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 8.8 0.8 unbinned pixels; 0.8 3.0 nm along two shift axes; -0.5 3.0 nm on specimen axes Rate = 15.45248 A/sec 4.6 0.8 unbinned pixels; 0.3 1.6 nm along two shift axes; -0.4 1.6 nm on specimen axes Rate = 8.28045 A/sec 2.5 1.3 unbinned pixels; 0.1 1.0 nm along two shift axes; -0.5 0.8 nm on specimen axes Rate = 4.97489 A/sec Drift is low enough after shot 3 Measured defocus = -4.70 microns changed by -0.05 to target drift = 0.06 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -2.181 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_012_2.0_Oct23_01.51.22.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 99.7 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.247 -0.196 IS units 287.0 858.6 unbinned pixels; need stage -0.304 0.074 if reset Tilting to 4 Correcting tilt backlash -17.1 0.3 unbinned pixels; 2.1 5.6 nm along two shift axes; 0.4 -6.0 nm on specimen axes Rate = 30.06388 A/sec -8.3 3.5 unbinned pixels; 2.1 2.3 nm along two shift axes; -1.0 -3.0 nm on specimen axes Rate = 15.77406 A/sec -8.2 2.4 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.6 -2.9 nm on specimen axes Rate = 14.87993 A/sec -6.7 2.3 unbinned pixels; 1.5 1.9 nm along two shift axes; -0.6 -2.4 nm on specimen axes Rate = 12.33812 A/sec -5.7 1.7 unbinned pixels; 1.2 1.7 nm along two shift axes; -0.4 -2.1 nm on specimen axes Rate = 10.44467 A/sec -4.2 1.8 unbinned pixels; 1.1 1.2 nm along two shift axes; -0.5 -1.5 nm on specimen axes Rate = 8.08356 A/sec -4.3 1.5 unbinned pixels; 1.0 1.2 nm along two shift axes; -0.4 -1.5 nm on specimen axes Rate = 7.92856 A/sec Drift is low enough after shot 7 Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -2.249 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_013_4.0_Oct23_01.52.55.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 90.9 In SetExposureForMean 90, change by a factor of 0.9905 would require too small a change in exposure time Image shift -0.241 -0.209 IS units 329.0 854.6 unbinned pixels; need stage -0.304 0.089 if reset Tilting to -24 -24.00 degrees -6.7 -2.0 unbinned pixels; 0.1 2.5 nm along two shift axes; 0.9 -2.3 nm on specimen axes Rate = 12.26903 A/sec 1.4 1.6 unbinned pixels; 0.4 0.6 nm along two shift axes; -0.6 0.4 nm on specimen axes Rate = 3.6558 A/sec Drift is low enough after shot 2 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.03 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -1.160 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_014_-24.0_Oct23_01.53.59.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 84.7 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.294 -0.140 IS units 89.4 934.5 unbinned pixels; need stage -0.324 0.001 if reset Tilting to -26 2.5 0.6 unbinned pixels; 0.1 0.9 nm along two shift axes; -0.3 0.9 nm on specimen axes Rate = 4.50011 A/sec Drift is low enough after shot 1 Measured defocus = -4.81 microns changed by 0.06 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.086 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_015_-26.0_Oct23_01.54.55.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 90.8 In SetExposureForMean 90, change by a factor of 0.9929 would require too small a change in exposure time Image shift -0.298 -0.132 IS units 62.9 936.2 unbinned pixels; need stage -0.324 -0.009 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 8.1 0.0 unbinned pixels; 0.9 2.7 nm along two shift axes; -0.3 2.8 nm on specimen axes Rate = 14.21648 A/sec 9.8 1.8 unbinned pixels; 0.5 3.5 nm along two shift axes; -0.9 3.4 nm on specimen axes Rate = 17.47939 A/sec 6.5 1.6 unbinned pixels; 0.2 2.3 nm along two shift axes; -0.8 2.2 nm on specimen axes Rate = 11.79248 A/sec 4.2 0.9 unbinned pixels; 0.2 1.5 nm along two shift axes; -0.5 1.4 nm on specimen axes Rate = 7.50673 A/sec Drift is low enough after shot 4 Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -2.303 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_016_6.0_Oct23_01.56.16.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 98.7 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.246 -0.261 IS units 465.3 917.2 unbinned pixels; need stage -0.330 0.136 if reset Tilting to 8 Correcting tilt backlash -30.7 5.9 unbinned pixels; 5.6 9.4 nm along two shift axes; -1.1 -10.9 nm on specimen axes Rate = 54.80138 A/sec -20.6 5.7 unbinned pixels; 4.3 6.1 nm along two shift axes; -1.4 -7.4 nm on specimen axes Rate = 37.46256 A/sec -13.5 3.2 unbinned pixels; 2.6 4.1 nm along two shift axes; -0.7 -4.8 nm on specimen axes Rate = 24.2643 A/sec -9.3 2.5 unbinned pixels; 1.9 2.8 nm along two shift axes; -0.6 -3.3 nm on specimen axes Rate = 16.91365 A/sec -8.5 2.1 unbinned pixels; 1.7 2.6 nm along two shift axes; -0.5 -3.1 nm on specimen axes Rate = 15.41524 A/sec -7.5 2.0 unbinned pixels; 1.5 2.2 nm along two shift axes; -0.5 -2.7 nm on specimen axes Rate = 13.54856 A/sec -5.8 1.7 unbinned pixels; 1.2 1.7 nm along two shift axes; -0.4 -2.1 nm on specimen axes Rate = 10.63741 A/sec -5.2 1.3 unbinned pixels; 1.0 1.6 nm along two shift axes; -0.3 -1.9 nm on specimen axes Rate = 9.45599 A/sec -4.1 1.4 unbinned pixels; 1.0 1.2 nm along two shift axes; -0.4 -1.5 nm on specimen axes Rate = 7.61695 A/sec Drift is low enough after shot 9 Measured defocus = -4.70 microns changed by -0.05 to target drift = 0.11 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -2.343 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_017_8.0_Oct23_01.58.00.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 89.7 In SetExposureForMean 90, change by a factor of 1.0031 would require too small a change in exposure time Image shift -0.249 -0.249 IS units 428.4 915.8 unbinned pixels; need stage -0.328 0.123 if reset Tilting to -28 -28.00 degrees -4.9 -1.2 unbinned pixels; 0.2 1.8 nm along two shift axes; 0.6 -1.7 nm on specimen axes Rate = 8.85861 A/sec 2.0 1.4 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.5 0.6 nm on specimen axes Rate = 4.21486 A/sec Drift is low enough after shot 2 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.02 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -1.015 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_018_-28.0_Oct23_01.59.04.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 81.7 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.291 -0.201 IS units 258.7 984.3 unbinned pixels; need stage -0.347 0.067 if reset Tilting to -30 0.9 -0.0 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.0 0.3 nm on specimen axes Rate = 1.62616 A/sec Drift is low enough after shot 1 Measured defocus = -4.80 microns changed by 0.05 to target drift = 0.06 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -0.982 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_019_-30.0_Oct23_02.00.00.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 87.7 In SetExposureForMean 90, change by a factor of 1.0287 would require too small a change in exposure time Image shift -0.301 -0.175 IS units 178.4 985.5 unbinned pixels; need stage -0.345 0.036 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -1.4 0.4 unbinned pixels; 0.3 0.4 nm along two shift axes; -0.1 -0.5 nm on specimen axes Rate = 2.4984 A/sec Drift is low enough after shot 1 Measured defocus = -4.72 microns changed by -0.03 to target drift = 0.11 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.380 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_020_10.0_Oct23_02.01.02.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 97.4 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.240 -0.322 IS units 635.1 958.3 unbinned pixels; need stage -0.349 0.196 if reset Tilting to 12 Correcting tilt backlash -30.9 6.1 unbinned pixels; 5.6 9.5 nm along two shift axes; -1.2 -11.0 nm on specimen axes Rate = 55.23866 A/sec -16.4 4.1 unbinned pixels; 3.3 5.0 nm along two shift axes; -0.9 -5.9 nm on specimen axes Rate = 29.69522 A/sec -10.5 2.2 unbinned pixels; 2.0 3.2 nm along two shift axes; -0.5 -3.8 nm on specimen axes Rate = 18.87369 A/sec -8.1 2.1 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.5 -2.9 nm on specimen axes Rate = 14.71388 A/sec -5.8 1.5 unbinned pixels; 1.2 1.7 nm along two shift axes; -0.3 -2.1 nm on specimen axes Rate = 10.4526 A/sec -4.9 1.5 unbinned pixels; 1.1 1.5 nm along two shift axes; -0.4 -1.8 nm on specimen axes Rate = 9.02092 A/sec -3.9 0.8 unbinned pixels; 0.7 1.2 nm along two shift axes; -0.2 -1.4 nm on specimen axes Rate = 6.92402 A/sec Drift is low enough after shot 7 Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.447 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_021_12.0_Oct23_02.02.34.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 88.3 In SetExposureForMean 90, change by a factor of 1.0193 would require too small a change in exposure time Image shift -0.241 -0.309 IS units 600.6 949.3 unbinned pixels; need stage -0.345 0.186 if reset Tilting to -32 -32.00 degrees -5.1 -0.7 unbinned pixels; 0.4 1.8 nm along two shift axes; 0.4 -1.8 nm on specimen axes Rate = 9.1169 A/sec 2.0 1.5 unbinned pixels; 0.3 0.8 nm along two shift axes; -0.6 0.7 nm on specimen axes Rate = 4.43758 A/sec Drift is low enough after shot 2 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.02 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.901 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_022_-32.0_Oct23_02.03.40.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 78.3 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.295 -0.232 IS units 339.1 1024.6 unbinned pixels; need stage -0.363 0.102 if reset Tilting to -34 1.4 -1.4 unbinned pixels; 0.6 0.3 nm along two shift axes; 0.5 0.5 nm on specimen axes Rate = 3.5705 A/sec Drift is low enough after shot 1 Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.04 nm/sec Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = -0.853 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_023_-34.0_Oct23_02.04.36.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 90.6 In SetExposureForMean 90, change by a factor of 0.9966 would require too small a change in exposure time Image shift -0.290 -0.236 IS units 352.9 1014.8 unbinned pixels; need stage -0.360 0.111 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees 1.6 -1.2 unbinned pixels; 0.6 0.4 nm along two shift axes; 0.4 0.6 nm on specimen axes Rate = 3.53706 A/sec Drift is low enough after shot 1 Measured defocus = -4.67 microns changed by -0.08 to target drift = 0.03 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -2.520 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_024_14.0_Oct23_02.05.39.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 103.3 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.231 -0.343 IS units 701.4 955.1 unbinned pixels; need stage -0.350 0.224 if reset Tilting to 16 Correcting tilt backlash -5.4 -0.5 unbinned pixels; 0.5 1.8 nm along two shift axes; 0.3 -1.9 nm on specimen axes Rate = 9.44486 A/sec -0.7 1.9 unbinned pixels; 0.7 0.0 nm along two shift axes; -0.7 -0.3 nm on specimen axes Rate = 3.58727 A/sec Drift is low enough after shot 2 Measured defocus = -4.68 microns changed by -0.07 to target drift = 0.08 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.586 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_025_16.0_Oct23_02.06.43.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 86.5 In SetExposureForMean 90, change by a factor of 1.0412 would require too small a change in exposure time Image shift -0.213 -0.371 IS units 795.6 932.4 unbinned pixels; need stage -0.345 0.261 if reset Tilting to -36 -36.00 degrees 1.1 1.8 unbinned pixels; 0.5 0.6 nm along two shift axes; -0.7 0.3 nm on specimen axes Rate = 3.68239 A/sec Drift is low enough after shot 1 Measured defocus = -4.80 microns changed by 0.05 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.790 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_026_-36.0_Oct23_02.07.44.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 74.4 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.296 -0.257 IS units 404.8 1051.0 unbinned pixels; need stage -0.374 0.135 if reset Tilting to -38 2.8 -3.8 unbinned pixels; 1.6 0.5 nm along two shift axes; 1.3 1.1 nm on specimen axes Rate = 8.35632 A/sec 5.3 1.9 unbinned pixels; 0.0 2.0 nm along two shift axes; -0.8 1.8 nm on specimen axes Rate = 9.9348 A/sec 1.5 1.3 unbinned pixels; 0.3 0.7 nm along two shift axes; -0.5 0.5 nm on specimen axes Rate = 3.54849 A/sec Drift is low enough after shot 3 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.06 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.738 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_027_-38.0_Oct23_02.08.52.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 85.6 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.276 -0.295 IS units 526.2 1032.1 unbinned pixels; need stage -0.371 0.194 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -9.9 5.2 unbinned pixels; 2.9 2.7 nm along two shift axes; -1.5 -3.6 nm on specimen axes Rate = 19.59378 A/sec -7.4 3.4 unbinned pixels; 2.0 2.0 nm along two shift axes; -1.0 -2.7 nm on specimen axes Rate = 14.16527 A/sec -4.2 2.8 unbinned pixels; 1.4 1.1 nm along two shift axes; -0.8 -1.6 nm on specimen axes Rate = 8.81792 A/sec -3.2 1.8 unbinned pixels; 1.0 0.8 nm along two shift axes; -0.5 -1.2 nm on specimen axes Rate = 6.37761 A/sec Drift is low enough after shot 4 Measured defocus = -4.64 microns changed by -0.11 to target drift = 0.10 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -2.700 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_028_18.0_Oct23_02.10.12.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 108.7 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.232 -0.364 IS units 759.0 977.4 unbinned pixels; need stage -0.360 0.249 if reset Tilting to 20 Correcting tilt backlash 1.9 -1.1 unbinned pixels; 0.6 0.5 nm along two shift axes; 0.3 0.7 nm on specimen axes Rate = 3.79727 A/sec Drift is low enough after shot 1 Measured defocus = -4.73 microns changed by -0.02 to target drift = 0.12 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.729 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_029_20.0_Oct23_02.11.11.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 92.0 In SetExposureForMean 90, change by a factor of 0.9801 would require too small a change in exposure time Image shift -0.206 -0.417 IS units 926.6 957.7 unbinned pixels; need stage -0.358 0.316 if reset Tilting to -40 -40.00 degrees 0.5 -3.3 unbinned pixels; 1.2 0.2 nm along two shift axes; 1.2 0.3 nm on specimen axes Rate = 5.95348 A/sec Drift is low enough after shot 1 Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.06 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.705 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_030_-40.0_Oct23_02.12.12.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 76.5 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.252 -0.392 IS units 813.6 1057.8 unbinned pixels; need stage -0.389 0.331 if reset Tilting to -42 1.4 -5.2 unbinned pixels; 1.9 0.2 nm along two shift axes; 1.8 0.7 nm on specimen axes Rate = 9.54541 A/sec 2.9 2.9 unbinned pixels; 0.6 1.3 nm along two shift axes; -1.1 0.9 nm on specimen axes Rate = 7.13948 A/sec Drift is low enough after shot 2 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.07 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.658 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_031_-42.0_Oct23_02.13.14.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 85.9 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.219 -0.451 IS units 1005.7 1024.0 unbinned pixels; need stage -0.383 0.434 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -18.9 3.4 unbinned pixels; 3.3 5.9 nm along two shift axes; -0.6 -6.7 nm on specimen axes Rate = 33.70634 A/sec -9.5 4.1 unbinned pixels; 2.5 2.7 nm along two shift axes; -1.1 -3.5 nm on specimen axes Rate = 18.15037 A/sec -6.9 0.2 unbinned pixels; 0.9 2.3 nm along two shift axes; 0.2 -2.4 nm on specimen axes Rate = 12.05544 A/sec -6.1 3.0 unbinned pixels; 1.7 1.7 nm along two shift axes; -0.9 -2.2 nm on specimen axes Rate = 11.87362 A/sec -4.4 1.6 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.27425 A/sec -4.0 1.7 unbinned pixels; 1.0 1.1 nm along two shift axes; -0.5 -1.5 nm on specimen axes Rate = 7.59386 A/sec Drift is low enough after shot 6 Measured defocus = -4.62 microns changed by -0.13 to target drift = 0.09 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -2.865 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_032_22.0_Oct23_02.14.46.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 112.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.240 -0.363 IS units 746.5 999.3 unbinned pixels; need stage -0.367 0.250 if reset Tilting to 24 Correcting tilt backlash -8.2 -0.2 unbinned pixels; 0.9 2.7 nm along two shift axes; 0.3 -2.9 nm on specimen axes Rate = 14.38258 A/sec -2.1 6.8 unbinned pixels; 2.5 0.1 nm along two shift axes; -2.3 -0.9 nm on specimen axes Rate = 12.51945 A/sec -1.4 3.4 unbinned pixels; 1.3 0.1 nm along two shift axes; -1.2 -0.6 nm on specimen axes Rate = 6.49662 A/sec Drift is low enough after shot 3 Measured defocus = -4.71 microns changed by -0.04 to target drift = 0.09 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.911 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_033_24.0_Oct23_02.15.56.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 88.8 In SetExposureForMean 90, change by a factor of 1.0169 would require too small a change in exposure time Image shift -0.198 -0.498 IS units 1153.3 1013.9 unbinned pixels; need stage -0.384 0.410 if reset Tilting to -44 -44.00 degrees 0.9 -3.4 unbinned pixels; 1.2 0.1 nm along two shift axes; 1.2 0.4 nm on specimen axes Rate = 6.16498 A/sec Drift is low enough after shot 1 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.07 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -0.627 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_034_-44.0_Oct23_02.16.57.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 70.8 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.192 -0.554 IS units 1312.7 1049.5 unbinned pixels; need stage -0.402 0.598 if reset Tilting to -46 2.6 -5.7 unbinned pixels; 2.2 0.2 nm along two shift axes; 1.9 1.1 nm on specimen axes Rate = 11.00469 A/sec 3.1 2.2 unbinned pixels; 0.4 1.3 nm along two shift axes; -0.9 1.0 nm on specimen axes Rate = 6.65581 A/sec Drift is low enough after shot 2 Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.592 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_035_-46.0_Oct23_02.17.59.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 84.6 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.161 -0.603 IS units 1474.8 1012.6 unbinned pixels; need stage -0.394 0.703 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -18.8 5.3 unbinned pixels; 3.9 5.6 nm along two shift axes; -1.3 -6.7 nm on specimen axes Rate = 34.14763 A/sec -10.8 4.2 unbinned pixels; 2.7 3.1 nm along two shift axes; -1.1 -3.9 nm on specimen axes Rate = 20.34111 A/sec -7.4 2.6 unbinned pixels; 1.7 2.1 nm along two shift axes; -0.7 -2.7 nm on specimen axes Rate = 13.66133 A/sec -5.7 2.2 unbinned pixels; 1.4 1.6 nm along two shift axes; -0.6 -2.1 nm on specimen axes Rate = 10.72302 A/sec -4.7 1.9 unbinned pixels; 1.2 1.3 nm along two shift axes; -0.5 -1.7 nm on specimen axes Rate = 8.87101 A/sec -5.6 0.8 unbinned pixels; 0.9 1.8 nm along two shift axes; -0.1 -2.0 nm on specimen axes Rate = 9.93092 A/sec -2.0 1.0 unbinned pixels; 0.6 0.6 nm along two shift axes; -0.3 -0.7 nm on specimen axes Rate = 3.91991 A/sec Drift is low enough after shot 7 Measured defocus = -4.61 microns changed by -0.14 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.10 nm/sec Defocus = -3.051 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_036_26.0_Oct23_02.19.37.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.226 -0.420 IS units 914.2 1015.0 unbinned pixels; need stage -0.377 0.323 if reset Tilting to 28 Correcting tilt backlash -4.0 -1.1 unbinned pixels; 0.1 1.5 nm along two shift axes; 0.5 -1.4 nm on specimen axes Rate = 7.33788 A/sec Drift is low enough after shot 1 Measured defocus = -4.72 microns changed by -0.03 to target drift = 0.14 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -3.078 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_037_28.0_Oct23_02.20.36.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 85.0 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.174 -0.545 IS units 1305.6 991.4 unbinned pixels; need stage -0.381 0.486 if reset Tilting to -48 -48.00 degrees 1.5 -5.5 unbinned pixels; 2.0 0.2 nm along two shift axes; 1.9 0.7 nm on specimen axes Rate = 9.94154 A/sec 1.4 3.2 unbinned pixels; 0.9 0.8 nm along two shift axes; -1.1 0.4 nm on specimen axes Rate = 6.07194 A/sec Drift is low enough after shot 2 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.03 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = -0.563 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_038_-48.0_Oct23_02.21.44.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 69.7 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift -0.111 -0.741 IS units 1897.3 1007.6 unbinned pixels; need stage -0.405 0.954 if reset Tilting to -50 0.4 -2.3 unbinned pixels; 0.8 0.1 nm along two shift axes; 0.8 0.2 nm on specimen axes Rate = 4.20546 A/sec Drift is low enough after shot 1 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.530 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_039_-50.0_Oct23_02.22.40.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 86.3 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift -0.092 -0.766 IS units 1984.6 978.7 unbinned pixels; need stage -0.398 1.042 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -17.5 3.0 unbinned pixels; 3.0 5.4 nm along two shift axes; -0.5 -6.2 nm on specimen axes Rate = 31.09126 A/sec -11.4 3.4 unbinned pixels; 2.5 3.4 nm along two shift axes; -0.8 -4.1 nm on specimen axes Rate = 20.90377 A/sec -13.9 4.4 unbinned pixels; 3.1 4.1 nm along two shift axes; -1.1 -5.0 nm on specimen axes Rate = 25.57578 A/sec -4.8 1.8 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.5 -1.7 nm on specimen axes Rate = 8.94371 A/sec -4.6 1.2 unbinned pixels; 0.9 1.4 nm along two shift axes; -0.3 -1.6 nm on specimen axes Rate = 8.30471 A/sec -2.7 0.9 unbinned pixels; 0.6 0.8 nm along two shift axes; -0.2 -1.0 nm on specimen axes Rate = 4.95806 A/sec Drift is low enough after shot 6 Measured defocus = -4.61 microns changed by -0.14 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.10 nm/sec Defocus = -3.220 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_040_30.0_Oct23_02.24.18.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 124.4 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.177 -0.529 IS units 1259.4 985.1 unbinned pixels; need stage -0.378 0.477 if reset Tilting to 32 Correcting tilt backlash -5.0 -2.2 unbinned pixels; 0.2 1.9 nm along two shift axes; 0.9 -1.7 nm on specimen axes Rate = 9.58132 A/sec -1.5 5.5 unbinned pixels; 2.0 0.2 nm along two shift axes; -1.9 -0.7 nm on specimen axes Rate = 10.01677 A/sec -1.7 2.7 unbinned pixels; 1.1 0.2 nm along two shift axes; -0.9 -0.7 nm on specimen axes Rate = 5.51057 A/sec Drift is low enough after shot 3 Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.08 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -3.231 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_041_32.0_Oct23_02.25.29.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 87.6 In SetExposureForMean 90, change by a factor of 1.0339 would require too small a change in exposure time Image shift -0.122 -0.661 IS units 1672.2 961.0 unbinned pixels; need stage -0.382 0.660 if reset Tilting to -52 -52.00 degrees -1.5 -4.3 unbinned pixels; 1.3 1.0 nm along two shift axes; 1.6 -0.4 nm on specimen axes Rate = 8.1279 A/sec 1.1 3.9 unbinned pixels; 1.2 0.8 nm along two shift axes; -1.4 0.3 nm on specimen axes Rate = 7.09098 A/sec Drift is low enough after shot 2 Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.08 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -0.496 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_042_-52.0_Oct23_02.26.38.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 62.2 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift -0.052 -0.884 IS units 2344.3 982.1 unbinned pixels; need stage -0.410 1.295 if reset Tilting to -54 1.0 -4.7 unbinned pixels; 1.7 0.2 nm along two shift axes; 1.6 0.5 nm on specimen axes Rate = 8.50296 A/sec 1.9 2.3 unbinned pixels; 0.5 0.9 nm along two shift axes; -0.9 0.6 nm on specimen axes Rate = 5.2774 A/sec Drift is low enough after shot 2 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.04 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -0.478 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_043_-54.0_Oct23_02.27.40.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 85.2 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift -0.029 -0.918 IS units 2457.7 952.9 unbinned pixels; need stage -0.404 1.426 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -17.4 2.6 unbinned pixels; 2.9 5.4 nm along two shift axes; -0.4 -6.2 nm on specimen axes Rate = 30.8719 A/sec -13.0 4.8 unbinned pixels; 3.1 3.7 nm along two shift axes; -1.3 -4.7 nm on specimen axes Rate = 24.30447 A/sec -6.5 1.8 unbinned pixels; 1.3 2.0 nm along two shift axes; -0.4 -2.3 nm on specimen axes Rate = 11.84697 A/sec -5.6 2.1 unbinned pixels; 1.4 1.6 nm along two shift axes; -0.6 -2.0 nm on specimen axes Rate = 10.53889 A/sec -5.7 1.8 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.5 -2.0 nm on specimen axes Rate = 10.41876 A/sec -3.4 1.5 unbinned pixels; 0.9 0.9 nm along two shift axes; -0.4 -1.2 nm on specimen axes Rate = 6.50212 A/sec Drift is low enough after shot 6 Measured defocus = -4.62 microns changed by -0.13 to target drift = 0.13 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -3.375 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_044_34.0_Oct23_02.29.18.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 130.7 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.127 -0.649 IS units 1631.9 962.9 unbinned pixels; need stage -0.382 0.658 if reset Tilting to 36 Correcting tilt backlash -6.0 -0.7 unbinned pixels; 0.5 2.1 nm along two shift axes; 0.4 -2.1 nm on specimen axes Rate = 10.62869 A/sec -1.0 5.5 unbinned pixels; 1.9 0.3 nm along two shift axes; -1.9 -0.5 nm on specimen axes Rate = 9.78509 A/sec -1.9 2.7 unbinned pixels; 1.1 0.3 nm along two shift axes; -0.9 -0.7 nm on specimen axes Rate = 5.72971 A/sec Drift is low enough after shot 3 Measured defocus = -4.73 microns changed by -0.02 to target drift = 0.09 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -3.395 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_045_36.0_Oct23_02.30.29.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 88.7 In SetExposureForMean 90, change by a factor of 1.0243 would require too small a change in exposure time Image shift -0.084 -0.760 IS units 1977.6 951.2 unbinned pixels; need stage -0.388 0.826 if reset Tilting to -56 -56.00 degrees 5.4 -7.1 unbinned pixels; 3.0 0.9 nm along two shift axes; 2.3 2.1 nm on specimen axes Rate = 15.61941 A/sec 4.2 2.7 unbinned pixels; 0.4 1.7 nm along two shift axes; -1.1 1.4 nm on specimen axes Rate = 8.77401 A/sec 0.3 2.5 unbinned pixels; 0.8 0.4 nm along two shift axes; -0.9 0.0 nm on specimen axes Rate = 4.41761 A/sec Drift is low enough after shot 3 Measured defocus = -4.71 microns changed by -0.04 to target drift = 0.06 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.506 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_046_-56.0_Oct23_02.31.43.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 58.4 In SetExposureForMean 90, exposure time changed to 3.5200 Image shift 0.018 -1.058 IS units 2883.4 959.3 unbinned pixels; need stage -0.419 1.768 if reset Tilting to -58 0.2 -2.6 unbinned pixels; 0.9 0.2 nm along two shift axes; 0.9 0.2 nm on specimen axes Rate = 4.63969 A/sec Drift is low enough after shot 1 Measured defocus = -4.72 microns changed by -0.03 to target drift = 0.07 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.518 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_047_-58.0_Oct23_02.32.39.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 84.3 In SetExposureForMean 90, exposure time changed to 3.8400 Image shift 0.053 -1.115 IS units 3073.6 918.5 unbinned pixels; need stage -0.411 1.995 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -19.4 3.3 unbinned pixels; 3.4 6.0 nm along two shift axes; -0.6 -6.9 nm on specimen axes Rate = 34.54766 A/sec -12.9 4.2 unbinned pixels; 2.9 3.8 nm along two shift axes; -1.1 -4.6 nm on specimen axes Rate = 23.69505 A/sec -6.5 2.3 unbinned pixels; 1.5 1.9 nm along two shift axes; -0.6 -2.4 nm on specimen axes Rate = 12.14995 A/sec -6.9 2.9 unbinned pixels; 1.8 1.9 nm along two shift axes; -0.8 -2.5 nm on specimen axes Rate = 13.03596 A/sec -5.0 1.7 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.4 -1.8 nm on specimen axes Rate = 9.19748 A/sec -4.1 2.5 unbinned pixels; 1.3 1.1 nm along two shift axes; -0.8 -1.5 nm on specimen axes Rate = 8.44855 A/sec -2.8 0.7 unbinned pixels; 0.6 0.8 nm along two shift axes; -0.2 -1.0 nm on specimen axes Rate = 5.03799 A/sec Drift is low enough after shot 7 Measured defocus = -4.61 microns changed by -0.14 to target drift = 0.14 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.10 nm/sec Defocus = -3.528 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_048_38.0_Oct23_02.34.19.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 146.9 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.092 -0.741 IS units 1915.3 956.1 unbinned pixels; need stage -0.388 0.820 if reset Tilting to 40 Correcting tilt backlash -6.6 -2.7 unbinned pixels; 0.1 2.5 nm along two shift axes; 1.1 -2.2 nm on specimen axes Rate = 12.50226 A/sec -2.9 3.8 unbinned pixels; 1.6 0.5 nm along two shift axes; -1.2 -1.1 nm on specimen axes Rate = 8.38904 A/sec -2.5 2.0 unbinned pixels; 1.0 0.6 nm along two shift axes; -0.6 -0.9 nm on specimen axes Rate = 5.55989 A/sec Drift is low enough after shot 3 Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.11 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -3.533 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_049_40.0_Oct23_02.35.30.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 88.3 In SetExposureForMean 90, change by a factor of 1.0327 would require too small a change in exposure time Image shift -0.048 -0.844 IS units 2239.4 935.1 unbinned pixels; need stage -0.391 0.994 if reset Tilting to -60 -60.00 degrees Trimming A by 25 25, reference by 20 20 -1.3 -2.4 unbinned pixels; 0.6 0.7 nm along two shift axes; 0.9 -0.4 nm on specimen axes Rate = 4.82751 A/sec Drift is low enough after shot 1 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.08 nm/sec Measured defocus = -4.73 microns changed by -0.02 to target drift = 0.05 nm/sec Defocus = -0.469 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_050_-60.0_Oct23_02.36.34.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 51.9 In SetExposureForMean 90, exposure time changed to 4.1600 Image shift 0.080 -1.207 IS units 3349.1 932.5 unbinned pixels; need stage -0.424 2.308 if reset Tilting to -62 Trimming A by 35 35, reference by 20 20 -2.7 -2.0 unbinned pixels; 0.3 1.1 nm along two shift axes; 0.8 -0.9 nm on specimen axes Rate = 5.99592 A/sec Drift is low enough after shot 1 Measured defocus = -4.71 microns changed by -0.04 to target drift = 0.08 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.496 um Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_051_-62.0_Oct23_02.37.31.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 80.7 In SetExposureForMean 90, exposure time changed to 4.6400 Image shift 0.098 -1.238 IS units 3450.9 910.6 unbinned pixels; need stage -0.420 2.536 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -20.6 3.1 unbinned pixels; 3.4 6.4 nm along two shift axes; -0.5 -7.3 nm on specimen axes Rate = 36.54301 A/sec -11.2 2.8 unbinned pixels; 2.2 3.4 nm along two shift axes; -0.6 -4.0 nm on specimen axes Rate = 20.16497 A/sec -8.6 3.0 unbinned pixels; 2.0 2.5 nm along two shift axes; -0.8 -3.1 nm on specimen axes Rate = 15.8901 A/sec -6.8 2.1 unbinned pixels; 1.5 2.0 nm along two shift axes; -0.5 -2.5 nm on specimen axes Rate = 12.54977 A/sec -5.4 1.5 unbinned pixels; 1.1 1.6 nm along two shift axes; -0.3 -1.9 nm on specimen axes Rate = 9.75004 A/sec -4.4 1.4 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.07465 A/sec -5.3 1.7 unbinned pixels; 1.2 1.5 nm along two shift axes; -0.4 -1.9 nm on specimen axes Rate = 9.70069 A/sec -4.0 1.1 unbinned pixels; 0.8 1.2 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 7.27136 A/sec Drift is low enough after shot 8 Measured defocus = -4.64 microns changed by -0.11 to target drift = 0.13 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -3.632 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_052_42.0_Oct23_02.39.17.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 163.6 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.064 -0.821 IS units 2162.6 955.7 unbinned pixels; need stage -0.395 0.987 if reset Tilting to 44 Correcting tilt backlash -8.8 -5.0 unbinned pixels; 0.6 3.5 nm along two shift axes; 2.0 -2.9 nm on specimen axes Rate = 17.88909 A/sec -3.3 4.3 unbinned pixels; 1.8 0.6 nm along two shift axes; -1.4 -1.3 nm on specimen axes Rate = 9.4533 A/sec -1.6 2.0 unbinned pixels; 0.8 0.3 nm along two shift axes; -0.6 -0.6 nm on specimen axes Rate = 4.47524 A/sec Drift is low enough after shot 3 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.12 nm/sec Measured defocus = -4.73 microns changed by -0.02 to target drift = 0.08 nm/sec Defocus = -3.630 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_053_44.0_Oct23_02.40.30.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 86.2 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift -0.027 -0.892 IS units 2389.0 922.4 unbinned pixels; need stage -0.391 1.133 if reset Tilting to -64 -64.00 degrees Trimming A by 85 85, reference by 30 30 Trimming A by 50 50, reference by 30 30 5.2 -7.0 unbinned pixels; 2.9 0.9 nm along two shift axes; 2.3 2.0 nm on specimen axes Rate = 15.26322 A/sec Trimming A by 50 50, reference by 30 30 2.1 6.4 unbinned pixels; 1.9 1.5 nm along two shift axes; -2.3 0.5 nm on specimen axes Rate = 11.97818 A/sec Trimming A by 50 50, reference by 30 30 3.8 -0.0 unbinned pixels; 0.4 1.3 nm along two shift axes; -0.1 1.3 nm on specimen axes Rate = 6.68771 A/sec Drift is low enough after shot 3 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.05 nm/sec Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.09 nm/sec Defocus = -0.444 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_054_-64.0_Oct23_02.41.51.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 46.1 In SetExposureForMean 90, exposure time changed to 5.2800 Image shift 0.124 -1.304 IS units 3654.4 904.1 unbinned pixels; need stage -0.424 2.881 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash -18.7 1.7 unbinned pixels; 2.7 6.0 nm along two shift axes; -0.0 -6.6 nm on specimen axes Rate = 32.99621 A/sec -12.3 3.5 unbinned pixels; 2.6 3.7 nm along two shift axes; -0.8 -4.4 nm on specimen axes Rate = 22.46329 A/sec -9.2 1.9 unbinned pixels; 1.7 2.8 nm along two shift axes; -0.4 -3.3 nm on specimen axes Rate = 16.5013 A/sec -6.1 2.3 unbinned pixels; 1.5 1.7 nm along two shift axes; -0.6 -2.2 nm on specimen axes Rate = 11.45472 A/sec -5.4 2.0 unbinned pixels; 1.3 1.6 nm along two shift axes; -0.5 -2.0 nm on specimen axes Rate = 10.13461 A/sec -5.6 1.1 unbinned pixels; 1.0 1.7 nm along two shift axes; -0.2 -2.0 nm on specimen axes Rate = 10.0162 A/sec -3.1 1.0 unbinned pixels; 0.7 0.9 nm along two shift axes; -0.3 -1.1 nm on specimen axes Rate = 5.70877 A/sec Drift is low enough after shot 7 Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.09 nm/sec Defocus = -3.687 um Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_055_46.0_Oct23_02.43.31.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 169.2 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift -0.044 -0.892 IS units 2372.1 968.7 unbinned pixels; need stage -0.406 1.162 if reset Tilting to 48 Correcting tilt backlash -9.0 -5.4 unbinned pixels; 0.7 3.6 nm along two shift axes; 2.2 -3.0 nm on specimen axes Rate = 18.45346 A/sec -2.6 4.5 unbinned pixels; 1.8 0.3 nm along two shift axes; -1.5 -1.1 nm on specimen axes Rate = 9.21808 A/sec -2.1 1.8 unbinned pixels; 0.9 0.5 nm along two shift axes; -0.6 -0.8 nm on specimen axes Rate = 4.84214 A/sec Drift is low enough after shot 3 Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.12 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -3.686 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_06_12_056_48.0_Oct23_02.44.49.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 87.7 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift 0.004 -0.967 IS units 2624.1 909.0 unbinned pixels; need stage -0.394 1.343 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -3.22 microns changed by -1.53 to target drift = 0.28 nm/sec Focus area changed from stored state, axis position 2.70 angle 0, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_07.st Running script 1 at item 14 (258.86, -45.69) 02:45:36 Refine ZLP: additional offset = 0.1 eV; total offset = 6.4 eV Item 14: Stage: 258.86 -45.69 -237 Label: 14 Note: Sec 12 - anchor_maps.map Target defocus is: -4.75 um Target defocus is: -4.00 um Target defocus = -4 Angle = -4.40, increment = 0.60, shift = -6.3 -3.0 pixels, -0.015 um Y Angle = -3.20, increment = 1.80, shift = -3.6 -5.4 pixels, -0.010 um Y Angle = 0.41, increment = 5.41, shift = -37.4 -13.7 pixels, -0.088 um Y Angle = 3.00, increment = 8.00, shift = -29.3 -15.8 pixels, -0.071 um Y Rough eucentricity: changing Z by 0.51 to -236.22, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = -11.5, 9.1 pixels, 0.024, -0.024 um, IS = -0.024, 0.024 Angle = 11.00, shift = -80.6, -7.6 pixels, 0.003, -0.185 um, IS = -0.027, 0.209 Angle = 4.00, shift = -78.0, -7.2 pixels, 0.004, -0.179 um, IS = -0.031, 0.387 Angle = -4.00, shift = 0.4, -15.2 pixels, -0.035, -0.003 um, IS = 0.004, 0.391 Angle = -11.00, shift = -15.3, -13.9 pixels, -0.028, -0.038 um, IS = 0.032, 0.429 Angle = -18.00, shift = 32.1, 9.0 pixels, 0.012, 0.075 um, IS = 0.019, 0.354 Angle = -24.00, shift = 9.2, 5.5 pixels, 0.010, 0.022 um, IS = 0.009, 0.332 General solution delZ 0.46, delY 2.33, yZero 0.37 solution with assumed YZero 0.39: delZ 0.46, delY 2.59 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.46 microns and lateral displacement of -2.33 microns Disparity in stage position after aligning to center of map frame: -0.26 -0.06 Aligning to target with image shift equivalent to stage shift: -0.27 -0.07 Disparity in stage position after aligning to map item itself: -0.26 -0.04 (align shift 0.00 -0.00) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 258.87 -45.64 -236.66 -0.8 1.0 unbinned pixels; 0.4 0.1 nm along two shift axes; -0.3 -0.3 nm on specimen axes Rate = 2.20608 A/sec Drift is low enough after shot 1 Measured defocus = -3.71 microns changed by -0.29 to target drift = 0.04 nm/sec Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.311 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_000_-10.0_Oct23_02.49.26.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 92.6 In SetExposureForMean 90, change by a factor of 0.9717 would require too small a change in exposure time Image shift -0.275 -0.048 IS units -140.7 794.7 unbinned pixels; need stage -0.269 -0.077 if reset Tilting to -8 Correcting tilt backlash 7.2 -0.0 unbinned pixels; 0.9 2.4 nm along two shift axes; -0.2 2.5 nm on specimen axes Rate = 12.57748 A/sec 6.4 2.1 unbinned pixels; 0.0 2.4 nm along two shift axes; -0.9 2.2 nm on specimen axes Rate = 11.88384 A/sec 3.3 0.5 unbinned pixels; 0.2 1.1 nm along two shift axes; -0.3 1.1 nm on specimen axes Rate = 5.84174 A/sec Drift is low enough after shot 3 Measured defocus = -3.85 microns changed by -0.15 to target drift = 0.05 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -0.463 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_001_-8.0_Oct23_02.50.36.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 93.2 In SetExposureForMean 90, change by a factor of 0.9658 would require too small a change in exposure time Image shift -0.326 -0.041 IS units -210.7 927.6 unbinned pixels; need stage -0.313 -0.106 if reset Tilting to -12 -12.00 degrees -6.3 0.9 unbinned pixels; 1.0 2.0 nm along two shift axes; -0.1 -2.2 nm on specimen axes Rate = 11.197 A/sec -1.9 4.5 unbinned pixels; 1.7 0.1 nm along two shift axes; -1.5 -0.8 nm on specimen axes Rate = 8.489 A/sec -2.3 1.1 unbinned pixels; 0.6 0.6 nm along two shift axes; -0.3 -0.8 nm on specimen axes Rate = 4.40812 A/sec Drift is low enough after shot 3 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.04 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -0.275 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_002_-12.0_Oct23_02.51.45.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 92.0 In SetExposureForMean 90, change by a factor of 0.9773 would require too small a change in exposure time Image shift -0.353 0.009 IS units -371.5 953.6 unbinned pixels; need stage -0.317 -0.166 if reset Tilting to -14 -0.1 0.4 unbinned pixels; 0.2 0.0 nm along two shift axes; -0.1 -0.1 nm on specimen axes Rate = 0.78673 A/sec Drift is low enough after shot 1 Measured defocus = -4.13 microns changed by 0.13 to target drift = 0.03 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.140 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_003_-14.0_Oct23_02.52.41.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 91.6 In SetExposureForMean 90, change by a factor of 0.9810 would require too small a change in exposure time Image shift -0.393 0.085 IS units -617.2 989.2 unbinned pixels; need stage -0.321 -0.258 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 9.7 -1.5 unbinned pixels; 1.6 3.0 nm along two shift axes; 0.2 3.4 nm on specimen axes Rate = 17.16709 A/sec 5.9 0.0 unbinned pixels; 0.7 1.9 nm along two shift axes; -0.2 2.1 nm on specimen axes Rate = 10.27449 A/sec 3.3 0.0 unbinned pixels; 0.4 1.1 nm along two shift axes; -0.1 1.2 nm on specimen axes Rate = 5.80967 A/sec Drift is low enough after shot 3 Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.12 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -0.543 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_004_-6.0_Oct23_02.53.52.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 93.2 In SetExposureForMean 90, change by a factor of 0.9655 would require too small a change in exposure time Image shift -0.305 -0.081 IS units -80.4 908.5 unbinned pixels; need stage -0.310 -0.058 if reset Tilting to -4 Correcting tilt backlash -4.7 -1.3 unbinned pixels; 0.1 1.7 nm along two shift axes; 0.6 -1.6 nm on specimen axes Rate = 8.64336 A/sec 0.1 1.0 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.3 0.0 nm on specimen axes Rate = 1.7507 A/sec Drift is low enough after shot 2 Measured defocus = -3.89 microns changed by -0.11 to target drift = 0.03 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = -0.661 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_005_-4.0_Oct23_02.54.56.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 93.6 In SetExposureForMean 90, change by a factor of 0.9612 would require too small a change in exposure time Image shift -0.287 -0.122 IS units 47.4 897.7 unbinned pixels; need stage -0.310 -0.012 if reset Tilting to -16 -16.00 degrees -8.5 -2.5 unbinned pixels; 0.2 3.1 nm along two shift axes; 1.1 -2.9 nm on specimen axes Rate = 15.49917 A/sec -0.4 1.7 unbinned pixels; 0.6 0.1 nm along two shift axes; -0.6 -0.2 nm on specimen axes Rate = 3.05345 A/sec Drift is low enough after shot 2 Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.06 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -0.095 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_006_-16.0_Oct23_02.56.01.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 90.6 In SetExposureForMean 90, change by a factor of 0.9916 would require too small a change in exposure time Image shift -0.380 0.031 IS units -459.3 1005.5 unbinned pixels; need stage -0.332 -0.203 if reset Tilting to -18 0.8 -2.2 unbinned pixels; 0.8 0.0 nm along two shift axes; 0.7 0.4 nm on specimen axes Rate = 4.13776 A/sec Drift is low enough after shot 1 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.05 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.005 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_007_-18.0_Oct23_02.56.58.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 89.9 In SetExposureForMean 90, change by a factor of 0.9998 would require too small a change in exposure time Image shift -0.368 0.006 IS units -378.2 996.6 unbinned pixels; need stage -0.331 -0.175 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 11.5 -0.5 unbinned pixels; 1.5 3.7 nm along two shift axes; -0.2 4.1 nm on specimen axes Rate = 20.2015 A/sec 4.1 -0.8 unbinned pixels; 0.7 1.3 nm along two shift axes; 0.2 1.5 nm on specimen axes Rate = 7.31074 A/sec Drift is low enough after shot 2 Measured defocus = -3.90 microns changed by -0.10 to target drift = 0.11 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -0.766 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_008_-2.0_Oct23_02.58.04.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 93.6 In SetExposureForMean 90, change by a factor of 0.9611 would require too small a change in exposure time Image shift -0.280 -0.165 IS units 170.3 918.8 unbinned pixels; need stage -0.321 0.030 if reset Tilting to 0 Correcting tilt backlash -8.8 2.1 unbinned pixels; 1.7 2.7 nm along two shift axes; -0.5 -3.1 nm on specimen axes Rate = 15.80597 A/sec -2.4 2.1 unbinned pixels; 1.0 0.5 nm along two shift axes; -0.7 -0.9 nm on specimen axes Rate = 5.51767 A/sec Drift is low enough after shot 2 Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.09 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -0.842 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_009_-0.0_Oct23_02.59.09.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 93.5 In SetExposureForMean 90, change by a factor of 0.9626 would require too small a change in exposure time Image shift -0.295 -0.128 IS units 57.7 924.7 unbinned pixels; need stage -0.320 -0.010 if reset Tilting to -20 -20.00 degrees -6.0 -6.3 unbinned pixels; 1.4 2.8 nm along two shift axes; 2.4 -1.9 nm on specimen axes Rate = 15.44483 A/sec 0.2 3.3 unbinned pixels; 1.1 0.5 nm along two shift axes; -1.1 -0.0 nm on specimen axes Rate = 5.7636 A/sec Drift is low enough after shot 2 Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.05 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = 0.008 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_010_-20.0_Oct23_03.00.13.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 88.7 In SetExposureForMean 90, change by a factor of 1.0122 would require too small a change in exposure time Image shift -0.342 -0.069 IS units -149.8 997.8 unbinned pixels; need stage -0.339 -0.091 if reset Tilting to -22 0.4 -2.1 unbinned pixels; 0.8 0.1 nm along two shift axes; 0.7 0.2 nm on specimen axes Rate = 3.82043 A/sec Drift is low enough after shot 1 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.06 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = 0.098 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_011_-22.0_Oct23_03.01.10.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 87.5 In SetExposureForMean 90, change by a factor of 1.0267 would require too small a change in exposure time Image shift -0.330 -0.090 IS units -82.4 984.1 unbinned pixels; need stage -0.336 -0.066 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 9.5 1.5 unbinned pixels; 0.6 3.3 nm along two shift axes; -0.8 3.3 nm on specimen axes Rate = 16.9471 A/sec 4.7 0.5 unbinned pixels; 0.4 1.6 nm along two shift axes; -0.3 1.6 nm on specimen axes Rate = 8.3612 A/sec 2.8 0.6 unbinned pixels; 0.1 1.0 nm along two shift axes; -0.3 1.0 nm on specimen axes Rate = 4.98864 A/sec Drift is low enough after shot 3 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -0.916 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_012_2.0_Oct23_03.02.22.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 93.5 In SetExposureForMean 90, change by a factor of 0.9627 would require too small a change in exposure time Image shift -0.313 -0.129 IS units 42.8 974.2 unbinned pixels; need stage -0.337 -0.016 if reset Tilting to 4 Correcting tilt backlash -21.1 5.3 unbinned pixels; 4.2 6.3 nm along two shift axes; -1.2 -7.6 nm on specimen axes Rate = 38.13108 A/sec -13.5 3.6 unbinned pixels; 2.8 4.0 nm along two shift axes; -0.9 -4.8 nm on specimen axes Rate = 24.4383 A/sec -9.7 0.7 unbinned pixels; 1.4 3.1 nm along two shift axes; 0.0 -3.4 nm on specimen axes Rate = 17.02209 A/sec -9.3 3.7 unbinned pixels; 2.3 2.6 nm along two shift axes; -1.0 -3.4 nm on specimen axes Rate = 17.50806 A/sec -7.4 2.1 unbinned pixels; 1.6 2.2 nm along two shift axes; -0.5 -2.7 nm on specimen axes Rate = 13.43662 A/sec -5.7 1.3 unbinned pixels; 1.1 1.7 nm along two shift axes; -0.3 -2.0 nm on specimen axes Rate = 10.1803 A/sec -5.0 1.4 unbinned pixels; 1.1 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 9.19289 A/sec -5.6 0.7 unbinned pixels; 0.9 1.8 nm along two shift axes; -0.1 -2.0 nm on specimen axes Rate = 9.86373 A/sec -5.1 0.6 unbinned pixels; 0.8 1.6 nm along two shift axes; -0.1 -1.8 nm on specimen axes Rate = 8.97529 A/sec -1.4 1.4 unbinned pixels; 0.6 0.3 nm along two shift axes; -0.4 -0.5 nm on specimen axes Rate = 3.49857 A/sec Drift is low enough after shot 10 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.11 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -0.993 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_013_4.0_Oct23_03.04.13.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 93.3 In SetExposureForMean 90, change by a factor of 0.9646 would require too small a change in exposure time Image shift -0.348 -0.056 IS units -191.9 1000.0 unbinned pixels; need stage -0.338 -0.101 if reset Tilting to -24 -24.00 degrees -4.5 -1.5 unbinned pixels; 0.0 1.7 nm along two shift axes; 0.7 -1.5 nm on specimen axes Rate = 8.3493 A/sec 0.8 0.8 unbinned pixels; 0.2 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.02591 A/sec Drift is low enough after shot 2 Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.03 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = 0.135 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_014_-24.0_Oct23_03.05.19.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 86.1 In SetExposureForMean 90, change by a factor of 1.0428 would require too small a change in exposure time Image shift -0.328 -0.130 IS units 28.8 1017.7 unbinned pixels; need stage -0.351 -0.025 if reset Tilting to -26 0.9 -2.3 unbinned pixels; 0.9 0.0 nm along two shift axes; 0.8 0.4 nm on specimen axes Rate = 4.28532 A/sec Drift is low enough after shot 1 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = 0.200 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_015_-26.0_Oct23_03.06.16.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 84.8 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.313 -0.153 IS units 105.5 998.4 unbinned pixels; need stage -0.347 0.006 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 6.5 -1.5 unbinned pixels; 1.3 2.0 nm along two shift axes; 0.3 2.3 nm on specimen axes Rate = 11.779 A/sec 6.8 0.4 unbinned pixels; 0.7 2.3 nm along two shift axes; -0.3 2.4 nm on specimen axes Rate = 12.00327 A/sec 4.1 0.4 unbinned pixels; 0.3 1.4 nm along two shift axes; -0.3 1.4 nm on specimen axes Rate = 7.18043 A/sec Drift is low enough after shot 3 Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.08 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -1.075 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_016_6.0_Oct23_03.07.29.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 102.4 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.335 -0.116 IS units -16.7 1022.1 unbinned pixels; need stage -0.351 -0.039 if reset Tilting to 8 Correcting tilt backlash -24.0 5.9 unbinned pixels; 4.8 7.3 nm along two shift axes; -1.3 -8.6 nm on specimen axes Rate = 43.39871 A/sec -18.5 4.9 unbinned pixels; 3.8 5.5 nm along two shift axes; -1.2 -6.6 nm on specimen axes Rate = 33.49743 A/sec -16.0 2.7 unbinned pixels; 2.7 5.0 nm along two shift axes; -0.4 -5.7 nm on specimen axes Rate = 28.41457 A/sec -8.8 3.6 unbinned pixels; 2.2 2.5 nm along two shift axes; -1.0 -3.2 nm on specimen axes Rate = 16.60262 A/sec -8.5 2.6 unbinned pixels; 1.8 2.5 nm along two shift axes; -0.6 -3.1 nm on specimen axes Rate = 15.58369 A/sec -7.5 2.0 unbinned pixels; 1.5 2.3 nm along two shift axes; -0.5 -2.7 nm on specimen axes Rate = 13.66354 A/sec -6.1 1.6 unbinned pixels; 1.2 1.8 nm along two shift axes; -0.4 -2.2 nm on specimen axes Rate = 11.00806 A/sec -5.3 1.5 unbinned pixels; 1.1 1.6 nm along two shift axes; -0.4 -1.9 nm on specimen axes Rate = 9.60221 A/sec -4.3 1.5 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.01107 A/sec -4.1 1.1 unbinned pixels; 0.9 1.2 nm along two shift axes; -0.3 -1.5 nm on specimen axes Rate = 7.51705 A/sec Drift is low enough after shot 10 Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.11 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -1.144 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_017_8.0_Oct23_03.09.19.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 92.6 In SetExposureForMean 90, change by a factor of 0.9717 would require too small a change in exposure time Image shift -0.349 -0.081 IS units -125.2 1027.5 unbinned pixels; need stage -0.350 -0.078 if reset Tilting to -28 -28.00 degrees -4.9 -2.5 unbinned pixels; 0.2 1.9 nm along two shift axes; 1.0 -1.6 nm on specimen axes Rate = 9.66432 A/sec 1.6 1.6 unbinned pixels; 0.3 0.7 nm along two shift axes; -0.6 0.5 nm on specimen axes Rate = 3.92697 A/sec Drift is low enough after shot 2 Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.02 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = 0.219 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_018_-28.0_Oct23_03.10.25.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 83.2 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.299 -0.223 IS units 310.8 1026.8 unbinned pixels; need stage -0.363 0.087 if reset Tilting to -30 2.8 -0.7 unbinned pixels; 0.5 0.8 nm along two shift axes; 0.2 1.0 nm on specimen axes Rate = 5.02542 A/sec Drift is low enough after shot 1 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = 0.277 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_019_-30.0_Oct23_03.11.21.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 89.7 In SetExposureForMean 90, change by a factor of 1.0001 would require too small a change in exposure time Image shift -0.292 -0.233 IS units 344.1 1017.8 unbinned pixels; need stage -0.361 0.102 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees 8.4 -1.5 unbinned pixels; 1.5 2.6 nm along two shift axes; 0.3 3.0 nm on specimen axes Rate = 14.94425 A/sec 9.4 2.9 unbinned pixels; 0.1 3.5 nm along two shift axes; -1.3 3.2 nm on specimen axes Rate = 17.32936 A/sec 3.8 2.4 unbinned pixels; 0.3 1.5 nm along two shift axes; -0.9 1.2 nm on specimen axes Rate = 7.85059 A/sec Drift is low enough after shot 3 Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.06 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -1.242 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_020_10.0_Oct23_03.12.36.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 101.4 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.331 -0.165 IS units 119.9 1059.4 unbinned pixels; need stage -0.368 0.008 if reset Tilting to 12 Correcting tilt backlash -31.6 7.5 unbinned pixels; 6.2 9.6 nm along two shift axes; -1.7 -11.3 nm on specimen axes Rate = 56.92972 A/sec -19.8 5.7 unbinned pixels; 4.2 5.9 nm along two shift axes; -1.4 -7.1 nm on specimen axes Rate = 36.04868 A/sec -12.8 3.5 unbinned pixels; 2.7 3.8 nm along two shift axes; -0.8 -4.6 nm on specimen axes Rate = 23.25445 A/sec -10.5 1.8 unbinned pixels; 1.8 3.2 nm along two shift axes; -0.3 -3.7 nm on specimen axes Rate = 18.58732 A/sec -5.4 2.8 unbinned pixels; 1.6 1.4 nm along two shift axes; -0.8 -2.0 nm on specimen axes Rate = 10.62069 A/sec -5.1 1.9 unbinned pixels; 1.2 1.4 nm along two shift axes; -0.5 -1.8 nm on specimen axes Rate = 9.45009 A/sec -4.0 1.5 unbinned pixels; 1.0 1.2 nm along two shift axes; -0.4 -1.5 nm on specimen axes Rate = 7.52188 A/sec Drift is low enough after shot 7 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.12 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -1.311 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_021_12.0_Oct23_03.14.10.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 91.7 In SetExposureForMean 90, change by a factor of 0.9817 would require too small a change in exposure time Image shift -0.341 -0.141 IS units 45.6 1061.0 unbinned pixels; need stage -0.367 -0.019 if reset Tilting to -32 -32.00 degrees -4.3 -1.1 unbinned pixels; 0.1 1.5 nm along two shift axes; 0.5 -1.5 nm on specimen axes Rate = 7.75729 A/sec Drift is low enough after shot 1 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = 0.323 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_022_-32.0_Oct23_03.15.10.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 79.8 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.290 -0.287 IS units 492.1 1063.1 unbinned pixels; need stage -0.381 0.165 if reset Tilting to -34 2.4 -5.9 unbinned pixels; 2.3 0.1 nm along two shift axes; 2.0 1.0 nm on specimen axes Rate = 11.26175 A/sec 3.4 2.9 unbinned pixels; 0.6 1.5 nm along two shift axes; -1.1 1.1 nm on specimen axes Rate = 7.97098 A/sec Drift is low enough after shot 2 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = 0.394 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_023_-34.0_Oct23_03.16.13.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 85.8 In SetExposureForMean 90, change by a factor of 1.0436 would require too small a change in exposure time Image shift -0.265 -0.336 IS units 648.0 1041.7 unbinned pixels; need stage -0.378 0.236 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -4.5 0.4 unbinned pixels; 0.7 1.4 nm along two shift axes; -0.0 -1.6 nm on specimen axes Rate = 7.93377 A/sec Drift is low enough after shot 1 Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.13 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -1.370 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_024_14.0_Oct23_03.17.16.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 100.3 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.324 -0.206 IS units 239.9 1077.5 unbinned pixels; need stage -0.378 0.051 if reset Tilting to 16 Correcting tilt backlash -23.3 0.7 unbinned pixels; 2.9 7.6 nm along two shift axes; 0.5 -8.2 nm on specimen axes Rate = 40.86808 A/sec -11.0 6.4 unbinned pixels; 3.4 2.9 nm along two shift axes; -1.9 -4.1 nm on specimen axes Rate = 22.32537 A/sec -7.6 2.7 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.7 -2.8 nm on specimen axes Rate = 14.14662 A/sec -5.8 1.9 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 10.74086 A/sec -7.6 2.7 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.7 -2.8 nm on specimen axes Rate = 14.1663 A/sec -3.5 1.2 unbinned pixels; 0.8 1.0 nm along two shift axes; -0.3 -1.3 nm on specimen axes Rate = 6.43396 A/sec Drift is low enough after shot 6 Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.09 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -1.445 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_025_16.0_Oct23_03.18.53.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 90.3 In SetExposureForMean 90, change by a factor of 0.9968 would require too small a change in exposure time Image shift -0.312 -0.224 IS units 300.3 1064.0 unbinned pixels; need stage -0.375 0.075 if reset Tilting to -36 -36.00 degrees -0.2 -4.6 unbinned pixels; 1.5 0.6 nm along two shift axes; 1.6 0.1 nm on specimen axes Rate = 8.21281 A/sec 0.3 3.2 unbinned pixels; 1.0 0.5 nm along two shift axes; -1.1 0.0 nm on specimen axes Rate = 5.64944 A/sec Drift is low enough after shot 2 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.03 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = 0.474 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_026_-36.0_Oct23_03.20.00.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 75.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.245 -0.426 IS units 911.6 1072.1 unbinned pixels; need stage -0.397 0.355 if reset Tilting to -38 2.4 -6.0 unbinned pixels; 2.3 0.1 nm along two shift axes; 2.0 1.0 nm on specimen axes Rate = 11.39825 A/sec 4.0 2.0 unbinned pixels; 0.2 1.6 nm along two shift axes; -0.8 1.3 nm on specimen axes Rate = 7.89197 A/sec Drift is low enough after shot 2 Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.04 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = 0.510 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_027_-38.0_Oct23_03.21.03.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 88.3 In SetExposureForMean 90, change by a factor of 1.0130 would require too small a change in exposure time Image shift -0.217 -0.471 IS units 1061.2 1039.1 unbinned pixels; need stage -0.390 0.433 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -10.1 4.4 unbinned pixels; 2.6 2.8 nm along two shift axes; -1.2 -3.7 nm on specimen axes Rate = 19.29214 A/sec -6.5 2.2 unbinned pixels; 1.5 1.9 nm along two shift axes; -0.6 -2.3 nm on specimen axes Rate = 11.95147 A/sec -3.9 1.9 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.6 -1.4 nm on specimen axes Rate = 7.67675 A/sec Drift is low enough after shot 3 Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.09 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -1.533 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_028_18.0_Oct23_03.22.18.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 107.4 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.326 -0.214 IS units 258.4 1091.5 unbinned pixels; need stage -0.384 0.059 if reset Tilting to 20 Correcting tilt backlash -6.1 -1.9 unbinned pixels; 0.1 2.3 nm along two shift axes; 0.8 -2.1 nm on specimen axes Rate = 11.27913 A/sec -3.4 5.3 unbinned pixels; 2.2 0.5 nm along two shift axes; -1.8 -1.4 nm on specimen axes Rate = 11.06824 A/sec -3.1 3.0 unbinned pixels; 1.4 0.7 nm along two shift axes; -1.0 -1.2 nm on specimen axes Rate = 7.59047 A/sec Drift is low enough after shot 3 Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -1.545 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_029_20.0_Oct23_03.23.28.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 88.5 In SetExposureForMean 90, change by a factor of 1.0180 would require too small a change in exposure time Image shift -0.280 -0.310 IS units 564.0 1057.9 unbinned pixels; need stage -0.381 0.176 if reset Tilting to -40 -40.00 degrees -0.2 -5.9 unbinned pixels; 1.9 0.8 nm along two shift axes; 2.1 0.1 nm on specimen axes Rate = 10.47796 A/sec -0.1 3.1 unbinned pixels; 1.0 0.3 nm along two shift axes; -1.1 -0.1 nm on specimen axes Rate = 5.46489 A/sec Drift is low enough after shot 2 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = 0.554 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_030_-40.0_Oct23_03.24.36.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 70.9 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.179 -0.588 IS units 1416.1 1045.6 unbinned pixels; need stage -0.403 0.609 if reset Tilting to -42 2.5 -6.5 unbinned pixels; 2.5 0.1 nm along two shift axes; 2.2 1.1 nm on specimen axes Rate = 12.35444 A/sec 3.5 2.1 unbinned pixels; 0.3 1.4 nm along two shift axes; -0.8 1.2 nm on specimen axes Rate = 7.18769 A/sec Drift is low enough after shot 2 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.03 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = 0.626 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_031_-42.0_Oct23_03.25.38.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 88.7 In SetExposureForMean 90, change by a factor of 1.0041 would require too small a change in exposure time Image shift -0.150 -0.636 IS units 1576.0 1014.9 unbinned pixels; need stage -0.398 0.706 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -19.7 2.0 unbinned pixels; 3.0 6.3 nm along two shift axes; -0.1 -7.0 nm on specimen axes Rate = 34.74098 A/sec -9.7 2.9 unbinned pixels; 2.1 2.9 nm along two shift axes; -0.7 -3.5 nm on specimen axes Rate = 17.79962 A/sec -8.5 1.9 unbinned pixels; 1.6 2.6 nm along two shift axes; -0.4 -3.0 nm on specimen axes Rate = 15.30042 A/sec -6.7 1.6 unbinned pixels; 1.3 2.0 nm along two shift axes; -0.3 -2.4 nm on specimen axes Rate = 12.12323 A/sec -5.2 1.3 unbinned pixels; 1.0 1.6 nm along two shift axes; -0.3 -1.9 nm on specimen axes Rate = 9.37173 A/sec -4.5 0.8 unbinned pixels; 0.8 1.4 nm along two shift axes; -0.2 -1.6 nm on specimen axes Rate = 7.98638 A/sec Drift is low enough after shot 6 Measured defocus = -3.87 microns changed by -0.13 to target drift = 0.11 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -1.682 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_032_22.0_Oct23_03.27.11.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 113.6 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.299 -0.266 IS units 427.0 1068.1 unbinned pixels; need stage -0.381 0.125 if reset Tilting to 24 Correcting tilt backlash -7.5 -0.8 unbinned pixels; 0.6 2.6 nm along two shift axes; 0.5 -2.6 nm on specimen axes Rate = 13.15812 A/sec -3.1 6.6 unbinned pixels; 2.6 0.2 nm along two shift axes; -2.2 -1.3 nm on specimen axes Rate = 12.81934 A/sec -2.0 3.2 unbinned pixels; 1.3 0.3 nm along two shift axes; -1.0 -0.8 nm on specimen axes Rate = 6.56154 A/sec Drift is low enough after shot 3 Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.07 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -1.681 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_033_24.0_Oct23_03.28.22.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 86.4 In SetExposureForMean 90, change by a factor of 1.0440 would require too small a change in exposure time Image shift -0.256 -0.384 IS units 789.0 1062.6 unbinned pixels; need stage -0.390 0.268 if reset Tilting to -44 -44.00 degrees -0.2 -6.9 unbinned pixels; 2.3 0.9 nm along two shift axes; 2.4 0.1 nm on specimen axes Rate = 12.22234 A/sec 0.7 3.2 unbinned pixels; 1.0 0.6 nm along two shift axes; -1.2 0.1 nm on specimen axes Rate = 5.86839 A/sec Drift is low enough after shot 2 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.03 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.02 nm/sec Defocus = 0.683 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_034_-44.0_Oct23_03.29.30.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 65.4 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.101 -0.768 IS units 1982.3 1006.0 unbinned pixels; need stage -0.407 0.929 if reset Tilting to -46 0.8 -7.8 unbinned pixels; 2.7 0.7 nm along two shift axes; 2.7 0.5 nm on specimen axes Rate = 13.83003 A/sec 5.1 3.6 unbinned pixels; 0.6 2.1 nm along two shift axes; -1.4 1.7 nm on specimen axes Rate = 10.9492 A/sec 1.2 0.5 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.1934 A/sec Drift is low enough after shot 3 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.02 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = 0.731 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_035_-46.0_Oct23_03.30.43.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 87.0 In SetExposureForMean 90, change by a factor of 1.0132 would require too small a change in exposure time Image shift -0.064 -0.836 IS units 2202.0 969.3 unbinned pixels; need stage -0.401 1.076 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -17.5 5.2 unbinned pixels; 3.8 5.2 nm along two shift axes; -1.3 -6.3 nm on specimen axes Rate = 31.97416 A/sec -8.4 4.4 unbinned pixels; 2.4 2.3 nm along two shift axes; -1.3 -3.1 nm on specimen axes Rate = 16.61368 A/sec -6.9 0.3 unbinned pixels; 0.9 2.2 nm along two shift axes; 0.1 -2.4 nm on specimen axes Rate = 12.06349 A/sec -6.4 3.8 unbinned pixels; 2.0 1.7 nm along two shift axes; -1.1 -2.4 nm on specimen axes Rate = 13.02561 A/sec -5.2 1.6 unbinned pixels; 1.1 1.5 nm along two shift axes; -0.4 -1.9 nm on specimen axes Rate = 9.54555 A/sec -4.3 1.6 unbinned pixels; 1.0 1.2 nm along two shift axes; -0.4 -1.5 nm on specimen axes Rate = 7.98874 A/sec Drift is low enough after shot 6 Measured defocus = -3.86 microns changed by -0.14 to target drift = 0.10 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -1.823 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_036_26.0_Oct23_03.32.20.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 119.0 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.289 -0.301 IS units 530.9 1073.4 unbinned pixels; need stage -0.386 0.170 if reset Tilting to 28 Correcting tilt backlash -7.6 -0.4 unbinned pixels; 0.8 2.5 nm along two shift axes; 0.4 -2.6 nm on specimen axes Rate = 13.28296 A/sec -2.9 6.7 unbinned pixels; 2.6 0.1 nm along two shift axes; -2.3 -1.2 nm on specimen axes Rate = 12.80644 A/sec -2.1 3.6 unbinned pixels; 1.4 0.3 nm along two shift axes; -1.2 -0.9 nm on specimen axes Rate = 7.35989 A/sec Drift is low enough after shot 3 Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.09 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -1.831 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_037_28.0_Oct23_03.33.31.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 92.2 In SetExposureForMean 90, change by a factor of 0.9791 would require too small a change in exposure time Image shift -0.247 -0.426 IS units 911.1 1079.0 unbinned pixels; need stage -0.399 0.325 if reset Tilting to -48 -48.00 degrees 1.1 -5.9 unbinned pixels; 2.1 0.3 nm along two shift axes; 2.0 0.6 nm on specimen axes Rate = 10.65759 A/sec 1.8 3.9 unbinned pixels; 1.1 1.1 nm along two shift axes; -1.4 0.5 nm on specimen axes Rate = 7.62989 A/sec Drift is low enough after shot 2 Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = 0.753 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_038_-48.0_Oct23_03.34.45.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 64.8 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.021 -0.960 IS units 2581.3 972.0 unbinned pixels; need stage -0.414 1.317 if reset Tilting to -50 -0.1 -7.1 unbinned pixels; 2.3 0.9 nm along two shift axes; 2.5 0.2 nm on specimen axes Rate = 12.45898 A/sec 4.2 3.1 unbinned pixels; 0.5 1.8 nm along two shift axes; -1.2 1.4 nm on specimen axes Rate = 9.27134 A/sec 1.5 1.1 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.4 0.5 nm on specimen axes Rate = 3.36906 A/sec Drift is low enough after shot 3 Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.03 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = 0.760 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_039_-50.0_Oct23_03.35.53.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 83.2 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift 0.015 -1.023 IS units 2787.2 932.8 unbinned pixels; need stage -0.407 1.486 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -16.5 5.7 unbinned pixels; 3.8 4.8 nm along two shift axes; -1.5 -6.0 nm on specimen axes Rate = 30.62436 A/sec -8.2 4.7 unbinned pixels; 2.5 2.2 nm along two shift axes; -1.4 -3.0 nm on specimen axes Rate = 16.53763 A/sec -7.2 2.6 unbinned pixels; 1.7 2.1 nm along two shift axes; -0.7 -2.6 nm on specimen axes Rate = 13.32238 A/sec -5.5 0.5 unbinned pixels; 0.8 1.8 nm along two shift axes; -0.0 -1.9 nm on specimen axes Rate = 9.67054 A/sec -5.0 3.2 unbinned pixels; 1.7 1.3 nm along two shift axes; -1.0 -1.9 nm on specimen axes Rate = 10.4881 A/sec -4.1 2.0 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.6 -1.5 nm on specimen axes Rate = 7.92729 A/sec Drift is low enough after shot 6 Measured defocus = -3.87 microns changed by -0.13 to target drift = 0.11 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -1.971 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_040_30.0_Oct23_03.37.27.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 131.9 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.278 -0.346 IS units 664.5 1087.7 unbinned pixels; need stage -0.395 0.231 if reset Tilting to 32 Correcting tilt backlash -8.3 -0.4 unbinned pixels; 0.9 2.8 nm along two shift axes; 0.4 -2.9 nm on specimen axes Rate = 14.6216 A/sec -3.7 6.9 unbinned pixels; 2.7 0.4 nm along two shift axes; -2.3 -1.5 nm on specimen axes Rate = 13.75731 A/sec -2.2 3.6 unbinned pixels; 1.4 0.3 nm along two shift axes; -1.2 -0.9 nm on specimen axes Rate = 7.34537 A/sec Drift is low enough after shot 3 Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.09 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -1.993 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_041_32.0_Oct23_03.38.38.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 88.9 In SetExposureForMean 90, change by a factor of 1.0185 would require too small a change in exposure time Image shift -0.237 -0.459 IS units 1011.2 1081.6 unbinned pixels; need stage -0.403 0.380 if reset Tilting to -52 -52.00 degrees 0.8 -7.6 unbinned pixels; 2.6 0.6 nm along two shift axes; 2.6 0.5 nm on specimen axes Rate = 13.45099 A/sec 2.0 4.0 unbinned pixels; 1.1 1.1 nm along two shift axes; -1.5 0.6 nm on specimen axes Rate = 7.83863 A/sec Drift is low enough after shot 2 Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = 0.793 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_042_-52.0_Oct23_03.39.47.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 56.7 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift 0.071 -1.184 IS units 3278.7 934.7 unbinned pixels; need stage -0.423 1.834 if reset Tilting to -54 0.3 -7.3 unbinned pixels; 2.5 0.8 nm along two shift axes; 2.6 0.3 nm on specimen axes Rate = 12.96355 A/sec 4.5 2.8 unbinned pixels; 0.4 1.8 nm along two shift axes; -1.1 1.5 nm on specimen axes Rate = 9.2893 A/sec 1.6 1.2 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.5 0.5 nm on specimen axes Rate = 3.55716 A/sec Drift is low enough after shot 3 Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.04 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = 0.800 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_043_-54.0_Oct23_03.40.55.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 80.4 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift 0.106 -1.240 IS units 3464.0 892.6 unbinned pixels; need stage -0.414 2.035 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -16.4 2.8 unbinned pixels; 2.9 5.1 nm along two shift axes; -0.5 -5.9 nm on specimen axes Rate = 29.24991 A/sec -8.9 4.0 unbinned pixels; 2.4 2.5 nm along two shift axes; -1.1 -3.2 nm on specimen axes Rate = 17.06349 A/sec -7.4 2.2 unbinned pixels; 1.6 2.2 nm along two shift axes; -0.5 -2.6 nm on specimen axes Rate = 13.44815 A/sec -6.2 2.0 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.2 nm on specimen axes Rate = 11.37306 A/sec -4.8 1.7 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.5 -1.7 nm on specimen axes Rate = 8.94549 A/sec -3.9 1.4 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.4 -1.4 nm on specimen axes Rate = 7.29865 A/sec Drift is low enough after shot 6 Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.11 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.114 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_044_34.0_Oct23_03.42.29.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 142.1 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.251 -0.428 IS units 912.9 1090.9 unbinned pixels; need stage -0.403 0.347 if reset Tilting to 36 Correcting tilt backlash -7.5 -0.9 unbinned pixels; 0.6 2.6 nm along two shift axes; 0.5 -2.6 nm on specimen axes Rate = 13.26323 A/sec -2.8 6.0 unbinned pixels; 2.3 0.2 nm along two shift axes; -2.0 -1.2 nm on specimen axes Rate = 11.58322 A/sec -2.1 2.4 unbinned pixels; 1.0 0.4 nm along two shift axes; -0.8 -0.8 nm on specimen axes Rate = 5.54978 A/sec Drift is low enough after shot 3 Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.07 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.121 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_045_36.0_Oct23_03.43.40.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 84.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.207 -0.517 IS units 1198.1 1054.7 unbinned pixels; need stage -0.400 0.481 if reset Tilting to -56 -56.00 degrees 2.9 -6.4 unbinned pixels; 2.5 0.2 nm along two shift axes; 2.1 1.2 nm on specimen axes Rate = 12.29661 A/sec 2.6 3.1 unbinned pixels; 0.7 1.2 nm along two shift axes; -1.2 0.8 nm on specimen axes Rate = 7.1328 A/sec Drift is low enough after shot 2 Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.04 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = 0.836 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_046_-56.0_Oct23_03.44.49.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 51.2 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift 0.143 -1.351 IS units 3801.8 896.1 unbinned pixels; need stage -0.425 2.352 if reset Tilting to -58 -0.2 -3.9 unbinned pixels; 1.3 0.5 nm along two shift axes; 1.4 0.1 nm on specimen axes Rate = 6.85236 A/sec Drift is low enough after shot 1 Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.05 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = 0.849 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_047_-58.0_Oct23_03.45.52.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 76.1 In SetExposureForMean 90, exposure time changed to 3.3600 Image shift 0.158 -1.367 IS units 3859.6 870.2 unbinned pixels; need stage -0.418 2.522 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -18.4 3.5 unbinned pixels; 3.3 5.7 nm along two shift axes; -0.7 -6.6 nm on specimen axes Rate = 32.86846 A/sec -9.9 3.6 unbinned pixels; 2.3 2.8 nm along two shift axes; -1.0 -3.6 nm on specimen axes Rate = 18.41509 A/sec -7.8 2.3 unbinned pixels; 1.7 2.3 nm along two shift axes; -0.6 -2.8 nm on specimen axes Rate = 14.2093 A/sec -6.3 1.6 unbinned pixels; 1.3 1.9 nm along two shift axes; -0.4 -2.3 nm on specimen axes Rate = 11.45376 A/sec -4.8 1.5 unbinned pixels; 1.0 1.4 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.74355 A/sec -4.3 1.1 unbinned pixels; 0.9 1.3 nm along two shift axes; -0.3 -1.6 nm on specimen axes Rate = 7.86782 A/sec Drift is low enough after shot 6 Measured defocus = -3.87 microns changed by -0.13 to target drift = 0.11 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.254 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_048_38.0_Oct23_03.47.27.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 158.1 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.226 -0.471 IS units 1053.0 1062.5 unbinned pixels; need stage -0.398 0.429 if reset Tilting to 40 Correcting tilt backlash -8.9 -4.3 unbinned pixels; 0.4 3.5 nm along two shift axes; 1.8 -3.0 nm on specimen axes Rate = 17.46875 A/sec -1.5 4.9 unbinned pixels; 1.8 0.1 nm along two shift axes; -1.7 -0.7 nm on specimen axes Rate = 8.95787 A/sec -2.5 1.7 unbinned pixels; 0.9 0.6 nm along two shift axes; -0.5 -0.9 nm on specimen axes Rate = 5.34779 A/sec Drift is low enough after shot 3 Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.08 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -2.262 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_049_40.0_Oct23_03.48.39.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 87.9 In SetExposureForMean 90, change by a factor of 1.0373 would require too small a change in exposure time Image shift -0.179 -0.560 IS units 1339.7 1021.3 unbinned pixels; need stage -0.393 0.575 if reset Tilting to -60 -60.00 degrees Trimming A by 20 25, reference by 20 20 -0.2 -4.2 unbinned pixels; 1.4 0.6 nm along two shift axes; 1.5 0.1 nm on specimen axes Rate = 7.48416 A/sec Drift is low enough after shot 1 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.08 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = 0.927 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_050_-60.0_Oct23_03.49.43.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 39.9 In SetExposureForMean 90, exposure time changed to 3.8400 Image shift 0.178 -1.419 IS units 4020.6 867.1 unbinned pixels; need stage -0.422 2.787 if reset Tilting to -62 Trimming A by 45 45, reference by 20 20 Trimming A by 25 25, reference by 20 20 -0.9 -4.3 unbinned pixels; 1.3 0.8 nm along two shift axes; 1.5 -0.2 nm on specimen axes Rate = 7.70338 A/sec Drift is low enough after shot 1 Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.06 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = 0.883 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_051_-62.0_Oct23_03.50.40.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 68.0 In SetExposureForMean 90, exposure time changed to 4.3200 Image shift 0.201 -1.457 IS units 4145.3 840.7 unbinned pixels; need stage -0.417 3.064 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -21.5 2.9 unbinned pixels; 3.5 6.8 nm along two shift axes; -0.4 -7.6 nm on specimen axes Rate = 38.13058 A/sec -10.2 3.9 unbinned pixels; 2.5 2.9 nm along two shift axes; -1.1 -3.7 nm on specimen axes Rate = 19.10513 A/sec -8.3 2.2 unbinned pixels; 1.7 2.5 nm along two shift axes; -0.5 -3.0 nm on specimen axes Rate = 15.04282 A/sec -6.8 2.1 unbinned pixels; 1.5 2.0 nm along two shift axes; -0.5 -2.4 nm on specimen axes Rate = 12.39175 A/sec -5.8 1.5 unbinned pixels; 1.2 1.7 nm along two shift axes; -0.3 -2.1 nm on specimen axes Rate = 10.45017 A/sec -4.9 1.5 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.4 -1.8 nm on specimen axes Rate = 8.96154 A/sec -4.3 1.4 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.3 -1.5 nm on specimen axes Rate = 7.86681 A/sec Drift is low enough after shot 7 Measured defocus = -3.85 microns changed by -0.15 to target drift = 0.11 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.10 nm/sec Defocus = -2.419 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_052_42.0_Oct23_03.52.22.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 191.1 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.198 -0.525 IS units 1227.7 1039.9 unbinned pixels; need stage -0.396 0.539 if reset Tilting to 44 Correcting tilt backlash -8.8 -4.8 unbinned pixels; 0.6 3.5 nm along two shift axes; 2.0 -2.9 nm on specimen axes Rate = 17.71297 A/sec -3.2 3.6 unbinned pixels; 1.6 0.6 nm along two shift axes; -1.2 -1.2 nm on specimen axes Rate = 8.48286 A/sec -2.8 1.7 unbinned pixels; 0.9 0.7 nm along two shift axes; -0.5 -1.0 nm on specimen axes Rate = 5.75302 A/sec Drift is low enough after shot 3 Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -2.430 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_053_44.0_Oct23_03.53.40.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 89.4 In SetExposureForMean 90, change by a factor of 1.0254 would require too small a change in exposure time Image shift -0.136 -0.638 IS units 1594.8 978.8 unbinned pixels; need stage -0.386 0.740 if reset Tilting to -64 -64.00 degrees Trimming A by 20 20, reference by 40 40 Trimming A by 50 50, reference by 40 40 0.7 -4.8 unbinned pixels; 1.7 0.3 nm along two shift axes; 1.7 0.4 nm on specimen axes Rate = 8.50561 A/sec Trimming A by 50 50, reference by 40 40 4.2 4.7 unbinned pixels; 1.1 1.9 nm along two shift axes; -1.8 1.3 nm on specimen axes Rate = 11.08209 A/sec Trimming A by 50 50, reference by 40 40 2.5 2.1 unbinned pixels; 0.4 1.1 nm along two shift axes; -0.8 0.8 nm on specimen axes Rate = 5.65759 A/sec Drift is low enough after shot 3 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.07 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = 0.966 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_054_-64.0_Oct23_03.54.56.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 30.1 In SetExposureForMean 90, exposure time changed to 5.2800 Trimming A by 30 30, reference by 20 20 Image shift 0.225 -1.515 IS units 4326.0 830.2 unbinned pixels; need stage -0.419 3.428 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash -10.7 2.4 unbinned pixels; 2.0 3.2 nm along two shift axes; -0.5 -3.8 nm on specimen axes Rate = 19.13477 A/sec -2.5 5.0 unbinned pixels; 1.9 0.2 nm along two shift axes; -1.7 -1.0 nm on specimen axes Rate = 9.77727 A/sec -2.0 3.2 unbinned pixels; 1.3 0.3 nm along two shift axes; -1.0 -0.8 nm on specimen axes Rate = 6.58255 A/sec Drift is low enough after shot 3 Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.08 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.488 um Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_055_46.0_Oct23_03.56.14.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 218.6 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.131 -0.669 IS units 1684.2 993.5 unbinned pixels; need stage -0.394 0.811 if reset Tilting to 48 Correcting tilt backlash -8.3 -4.4 unbinned pixels; 0.5 3.3 nm along two shift axes; 1.8 -2.8 nm on specimen axes Rate = 16.51075 A/sec -1.1 5.3 unbinned pixels; 1.9 0.3 nm along two shift axes; -1.8 -0.5 nm on specimen axes Rate = 9.48229 A/sec -2.2 2.2 unbinned pixels; 1.0 0.5 nm along two shift axes; -0.7 -0.8 nm on specimen axes Rate = 5.51449 A/sec Drift is low enough after shot 3 Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.07 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -2.519 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_07_14_056_48.0_Oct23_03.57.33.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 88.9 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.085 -0.753 IS units 1956.4 948.1 unbinned pixels; need stage -0.386 0.988 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -3.75 microns changed by -0.25 to target drift = 0.35 nm/sec Focus area changed from stored state, axis position -2.70 angle 10, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_08.st Running script 1 at item 16 (138.41, 115.63) 03:58:29 Refine ZLP: additional offset = 0.9 eV; total offset = 7.4 eV Item 16: Stage: 138.41 115.63 -235 Label: 16 Note: Sec 14 - anchor_maps.map Target defocus is: -4.00 um Target defocus is: -4.25 um Target defocus = -4.25 Trimming A by 46 75, reference by 46 75 Angle = -4.40, increment = 0.60, shift = -6.7 -4.3 pixels, -0.016 um Y Trimming A by 45 75, reference by 46 75 Angle = -3.20, increment = 1.80, shift = -16.8 -8.3 pixels, -0.040 um Y Trimming A by 41 75, reference by 46 75 Angle = 0.41, increment = 5.41, shift = -29.1 -14.6 pixels, -0.070 um Y Trimming A by 36 75, reference by 46 75 Angle = 3.00, increment = 8.00, shift = -31.5 -17.8 pixels, -0.076 um Y Rough eucentricity: changing Z by 0.55 to -234.41, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 45 70, reference by 41 70 Angle = 18.00, shift = -58.0, 4.2 pixels, 0.024, -0.130 um, IS = -0.024, 0.130 Trimming A by 36 75, reference by 37 75 Angle = 11.00, shift = -66.4, -5.1 pixels, 0.005, -0.152 um, IS = -0.030, 0.283 Trimming A by 27 75, reference by 36 75 Angle = 4.00, shift = -1.0, 5.9 pixels, 0.014, -0.001 um, IS = -0.043, 0.283 Trimming A by 66 24 50 55, reference by 61 24 45 55 Angle = -4.00, shift = -36.3, -19.9 pixels, -0.036, -0.088 um, IS = -0.007, 0.371 Trimming A by 60 60, reference by 37 75 Angle = -11.00, shift = 11.3, -4.7 pixels, -0.014, 0.024 um, IS = 0.006, 0.346 Trimming A by 56 80, reference by 60 60 Angle = -18.00, shift = -25.3, 1.4 pixels, 0.010, -0.057 um, IS = -0.004, 0.403 Trimming A by 65 80, reference by 60 80 Angle = -24.00, shift = -11.4, 6.9 pixels, 0.019, -0.024 um, IS = -0.022, 0.427 General solution delZ 0.46, delY 1.05, yZero 0.33 solution with assumed YZero 0.33: delZ 0.46, delY 0.94 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.46 microns and lateral displacement of -1.05 microns Trimming A by 90 24 40 65, reference by 70 24 30 75 Disparity in stage position after aligning to center of map frame: -0.14 -0.36 Aligning to target with image shift equivalent to stage shift: -0.15 -0.37 Disparity in stage position after aligning to map item itself: -0.14 -0.33 (align shift 0.00 0.00) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 138.43 115.67 -234.85 -1.7 -1.2 unbinned pixels; 0.2 0.7 nm along two shift axes; 0.5 -0.6 nm on specimen axes Rate = 3.72655 A/sec Drift is low enough after shot 1 Measured defocus = -3.60 microns changed by -0.65 to target drift = 0.11 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.10 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.10 nm/sec Defocus = -0.665 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_000_-10.0_Oct23_04.02.19.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 72.5 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.283 0.260 IS units -983.9 522.6 unbinned pixels; need stage -0.150 -0.370 if reset Tilting to -8 Correcting tilt backlash 1.7 -1.3 unbinned pixels; 0.6 0.4 nm along two shift axes; 0.4 0.6 nm on specimen axes Rate = 3.76306 A/sec Drift is low enough after shot 1 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.09 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -0.791 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_001_-8.0_Oct23_04.03.18.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 87.2 In SetExposureForMean 90, change by a factor of 1.0323 would require too small a change in exposure time Image shift -0.331 0.304 IS units -1150.8 612.9 unbinned pixels; need stage -0.175 -0.431 if reset Tilting to -12 -12.00 degrees -6.8 -2.2 unbinned pixels; 0.1 2.5 nm along two shift axes; 1.0 -2.3 nm on specimen axes Rate = 12.65526 A/sec -1.8 0.4 unbinned pixels; 0.3 0.6 nm along two shift axes; -0.1 -0.6 nm on specimen axes Rate = 3.22912 A/sec Drift is low enough after shot 2 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.14 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.12 nm/sec Defocus = -0.605 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_002_-12.0_Oct23_04.04.22.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 86.5 In SetExposureForMean 90, change by a factor of 1.0405 would require too small a change in exposure time Image shift -0.339 0.350 IS units -1283.0 589.5 unbinned pixels; need stage -0.163 -0.483 if reset Tilting to -14 -4.8 -2.0 unbinned pixels; 0.1 1.8 nm along two shift axes; 0.9 -1.6 nm on specimen axes Rate = 9.09277 A/sec 1.4 -1.0 unbinned pixels; 0.5 0.4 nm along two shift axes; 0.3 0.5 nm on specimen axes Rate = 3.08783 A/sec Drift is low enough after shot 2 Measured defocus = -4.40 microns changed by 0.15 to target drift = 0.10 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -0.471 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_003_-14.0_Oct23_04.05.25.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 86.1 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.364 0.428 IS units -1519.4 584.0 unbinned pixels; need stage -0.154 -0.573 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 7.9 -7.7 unbinned pixels; 3.5 1.7 nm along two shift axes; 2.5 3.0 nm on specimen axes Rate = 19.38641 A/sec 3.7 -1.7 unbinned pixels; 1.0 1.0 nm along two shift axes; 0.5 1.3 nm on specimen axes Rate = 7.10712 A/sec Drift is low enough after shot 2 Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.12 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.12 nm/sec Defocus = -0.895 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_004_-6.0_Oct23_04.06.32.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 94.7 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.273 0.288 IS units -1048.1 468.1 unbinned pixels; need stage -0.129 -0.388 if reset Tilting to -4 Correcting tilt backlash -6.3 -6.1 unbinned pixels; 1.3 2.8 nm along two shift axes; 2.3 -2.0 nm on specimen axes Rate = 15.55324 A/sec -0.9 -0.0 unbinned pixels; 0.1 0.3 nm along two shift axes; 0.0 -0.3 nm on specimen axes Rate = 1.49923 A/sec Drift is low enough after shot 2 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.09 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -1.022 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_005_-4.0_Oct23_04.07.37.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 87.4 In SetExposureForMean 90, change by a factor of 1.0302 would require too small a change in exposure time Image shift -0.247 0.274 IS units -984.5 411.2 unbinned pixels; need stage -0.111 -0.362 if reset Tilting to -16 -16.00 degrees -7.4 -7.4 unbinned pixels; 1.6 3.3 nm along two shift axes; 2.8 -2.4 nm on specimen axes Rate = 18.47956 A/sec -2.4 0.0 unbinned pixels; 0.3 0.8 nm along two shift axes; 0.1 -0.9 nm on specimen axes Rate = 4.28432 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.10 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -0.419 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_006_-16.0_Oct23_04.08.42.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 85.5 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.305 0.394 IS units -1367.7 454.4 unbinned pixels; need stage -0.114 -0.518 if reset Tilting to -18 2.2 -1.9 unbinned pixels; 0.9 0.5 nm along two shift axes; 0.6 0.8 nm on specimen axes Rate = 5.10747 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -0.335 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_007_-18.0_Oct23_04.09.39.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 91.9 In SetExposureForMean 90, change by a factor of 0.9797 would require too small a change in exposure time Image shift -0.293 0.397 IS units -1363.4 420.0 unbinned pixels; need stage -0.103 -0.521 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 3.3 -7.9 unbinned pixels; 3.0 0.1 nm along two shift axes; 2.7 1.4 nm on specimen axes Rate = 15.09059 A/sec 4.7 0.6 unbinned pixels; 0.4 1.6 nm along two shift axes; -0.3 1.6 nm on specimen axes Rate = 8.34006 A/sec 1.9 -0.6 unbinned pixels; 0.4 0.6 nm along two shift axes; 0.1 0.7 nm on specimen axes Rate = 3.5084 A/sec Drift is low enough after shot 3 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.04 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -1.093 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_008_-2.0_Oct23_04.10.53.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 94.5 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.178 0.223 IS units -778.9 273.2 unbinned pixels; need stage -0.070 -0.284 if reset Tilting to 0 Correcting tilt backlash -5.6 -0.3 unbinned pixels; 0.6 1.9 nm along two shift axes; 0.3 -2.0 nm on specimen axes Rate = 9.85707 A/sec -3.0 -0.6 unbinned pixels; 0.2 1.1 nm along two shift axes; 0.3 -1.0 nm on specimen axes Rate = 5.43219 A/sec Drift is low enough after shot 2 Measured defocus = -4.10 microns changed by -0.15 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -1.234 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_009_-0.0_Oct23_04.11.59.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 86.8 In SetExposureForMean 90, change by a factor of 1.0364 would require too small a change in exposure time Image shift -0.182 0.246 IS units -845.4 262.5 unbinned pixels; need stage -0.064 -0.307 if reset Tilting to -20 -20.00 degrees -4.8 -6.9 unbinned pixels; 1.7 2.4 nm along two shift axes; 2.6 -1.5 nm on specimen axes Rate = 14.9157 A/sec -0.3 0.7 unbinned pixels; 0.3 0.0 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.31465 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.05 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -0.286 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_010_-20.0_Oct23_04.13.05.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 83.9 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.237 0.342 IS units -1159.6 320.8 unbinned pixels; need stage -0.075 -0.447 if reset Tilting to -22 2.4 -0.1 unbinned pixels; 0.3 0.8 nm along two shift axes; -0.0 0.8 nm on specimen axes Rate = 4.18597 A/sec Drift is low enough after shot 1 Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.04 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -0.226 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_011_-22.0_Oct23_04.14.07.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 89.8 In SetExposureForMean 90, change by a factor of 1.0028 would require too small a change in exposure time Image shift -0.230 0.340 IS units -1146.7 302.4 unbinned pixels; need stage -0.069 -0.448 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 5.9 -0.3 unbinned pixels; 0.8 1.9 nm along two shift axes; -0.1 2.1 nm on specimen axes Rate = 10.36177 A/sec 5.9 0.4 unbinned pixels; 0.6 2.0 nm along two shift axes; -0.3 2.0 nm on specimen axes Rate = 10.31971 A/sec 2.5 -0.3 unbinned pixels; 0.4 0.8 nm along two shift axes; 0.0 0.9 nm on specimen axes Rate = 4.40418 A/sec Drift is low enough after shot 3 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.04 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.02 nm/sec Defocus = -1.335 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_012_2.0_Oct23_04.15.21.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 93.6 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.190 0.282 IS units -951.1 247.5 unbinned pixels; need stage -0.056 -0.345 if reset Tilting to 4 Correcting tilt backlash -8.0 0.9 unbinned pixels; 1.2 2.5 nm along two shift axes; -0.1 -2.8 nm on specimen axes Rate = 14.04891 A/sec -6.9 1.7 unbinned pixels; 1.4 2.1 nm along two shift axes; -0.4 -2.5 nm on specimen axes Rate = 12.37764 A/sec -4.6 0.7 unbinned pixels; 0.8 1.4 nm along two shift axes; -0.1 -1.6 nm on specimen axes Rate = 8.1523 A/sec -7.2 0.8 unbinned pixels; 1.1 2.3 nm along two shift axes; -0.1 -2.5 nm on specimen axes Rate = 12.65284 A/sec -2.2 0.2 unbinned pixels; 0.3 0.7 nm along two shift axes; 0.0 -0.8 nm on specimen axes Rate = 3.81624 A/sec Drift is low enough after shot 5 Measured defocus = -4.14 microns changed by -0.11 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -1.444 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_013_4.0_Oct23_04.16.48.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 85.9 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.192 0.310 IS units -1030.1 226.9 unbinned pixels; need stage -0.046 -0.372 if reset Tilting to -24 -24.00 degrees -2.6 -5.4 unbinned pixels; 1.5 1.5 nm along two shift axes; 2.0 -0.8 nm on specimen axes Rate = 10.64526 A/sec -0.5 2.5 unbinned pixels; 0.9 0.1 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.44887 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.01 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = -0.180 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_014_-24.0_Oct23_04.17.55.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 88.7 In SetExposureForMean 90, change by a factor of 1.0153 would require too small a change in exposure time Image shift -0.191 0.277 IS units -939.5 256.6 unbinned pixels; need stage -0.059 -0.373 if reset Tilting to -26 2.8 0.0 unbinned pixels; 0.3 0.9 nm along two shift axes; -0.1 1.0 nm on specimen axes Rate = 4.93272 A/sec Drift is low enough after shot 1 Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.03 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.141 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_015_-26.0_Oct23_04.18.52.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 87.2 In SetExposureForMean 90, change by a factor of 1.0324 would require too small a change in exposure time Image shift -0.179 0.256 IS units -868.7 244.2 unbinned pixels; need stage -0.057 -0.351 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 5.4 1.5 unbinned pixels; 0.1 2.0 nm along two shift axes; -0.7 1.9 nm on specimen axes Rate = 9.91441 A/sec 5.0 1.0 unbinned pixels; 0.2 1.8 nm along two shift axes; -0.5 1.7 nm on specimen axes Rate = 8.94699 A/sec 2.2 1.2 unbinned pixels; 0.1 0.9 nm along two shift axes; -0.5 0.7 nm on specimen axes Rate = 4.4921 A/sec Drift is low enough after shot 3 Measured defocus = -4.14 microns changed by -0.11 to target drift = 0.05 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -1.552 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_016_6.0_Oct23_04.20.07.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 92.3 In SetExposureForMean 90, change by a factor of 0.9746 would require too small a change in exposure time Image shift -0.212 0.352 IS units -1162.8 241.0 unbinned pixels; need stage -0.047 -0.421 if reset Tilting to 8 Correcting tilt backlash -17.5 2.0 unbinned pixels; 2.7 5.5 nm along two shift axes; -0.2 -6.2 nm on specimen axes Rate = 30.89588 A/sec -11.9 2.8 unbinned pixels; 2.3 3.6 nm along two shift axes; -0.6 -4.3 nm on specimen axes Rate = 21.45105 A/sec -7.8 2.2 unbinned pixels; 1.7 2.3 nm along two shift axes; -0.5 -2.8 nm on specimen axes Rate = 14.24823 A/sec -7.2 1.8 unbinned pixels; 1.5 2.2 nm along two shift axes; -0.4 -2.6 nm on specimen axes Rate = 13.07139 A/sec -5.1 1.1 unbinned pixels; 1.0 1.6 nm along two shift axes; -0.2 -1.8 nm on specimen axes Rate = 9.13381 A/sec -5.4 1.5 unbinned pixels; 1.1 1.6 nm along two shift axes; -0.4 -1.9 nm on specimen axes Rate = 9.8581 A/sec -2.9 0.5 unbinned pixels; 0.5 0.9 nm along two shift axes; -0.1 -1.0 nm on specimen axes Rate = 5.09541 A/sec Drift is low enough after shot 7 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.11 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -1.655 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_017_8.0_Oct23_04.21.41.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 91.7 In SetExposureForMean 90, change by a factor of 0.9814 would require too small a change in exposure time Image shift -0.210 0.365 IS units -1196.6 225.5 unbinned pixels; need stage -0.041 -0.435 if reset Tilting to -28 -28.00 degrees -4.9 -5.5 unbinned pixels; 1.3 2.3 nm along two shift axes; 2.1 -1.5 nm on specimen axes Rate = 12.99885 A/sec 2.2 2.3 unbinned pixels; 0.5 1.0 nm along two shift axes; -0.9 0.7 nm on specimen axes Rate = 5.63108 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.02 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -0.056 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_018_-28.0_Oct23_04.22.48.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 86.1 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.136 0.167 IS units -585.8 211.1 unbinned pixels; need stage -0.055 -0.242 if reset Tilting to -30 0.3 -1.6 unbinned pixels; 0.6 0.1 nm along two shift axes; 0.5 0.2 nm on specimen axes Rate = 2.85403 A/sec Drift is low enough after shot 1 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.03 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = 0.012 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_019_-30.0_Oct23_04.23.50.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 90.7 In SetExposureForMean 90, change by a factor of 0.9936 would require too small a change in exposure time Image shift -0.116 0.123 IS units -448.7 199.5 unbinned pixels; need stage -0.055 -0.191 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees 4.6 2.0 unbinned pixels; 0.1 1.8 nm along two shift axes; -0.9 1.6 nm on specimen axes Rate = 8.86159 A/sec 4.2 2.3 unbinned pixels; 0.3 1.7 nm along two shift axes; -1.0 1.4 nm on specimen axes Rate = 8.50398 A/sec 1.1 2.7 unbinned pixels; 0.8 0.7 nm along two shift axes; -1.0 0.3 nm on specimen axes Rate = 5.13528 A/sec Drift is low enough after shot 3 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.03 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -1.750 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_020_10.0_Oct23_04.25.10.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 97.8 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.233 0.404 IS units -1324.5 249.7 unbinned pixels; need stage -0.045 -0.484 if reset Tilting to 12 Correcting tilt backlash -21.1 4.6 unbinned pixels; 4.0 6.4 nm along two shift axes; -0.9 -7.5 nm on specimen axes Rate = 37.79815 A/sec -13.7 3.4 unbinned pixels; 2.7 4.1 nm along two shift axes; -0.8 -4.9 nm on specimen axes Rate = 24.6446 A/sec -16.8 4.4 unbinned pixels; 3.4 5.0 nm along two shift axes; -1.0 -6.0 nm on specimen axes Rate = 30.4361 A/sec -6.4 1.4 unbinned pixels; 1.2 2.0 nm along two shift axes; -0.3 -2.3 nm on specimen axes Rate = 11.55909 A/sec -4.6 1.2 unbinned pixels; 0.9 1.4 nm along two shift axes; -0.3 -1.6 nm on specimen axes Rate = 8.26152 A/sec -5.1 1.3 unbinned pixels; 1.0 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 9.17122 A/sec -3.2 0.2 unbinned pixels; 0.5 1.0 nm along two shift axes; 0.0 -1.1 nm on specimen axes Rate = 5.71582 A/sec Drift is low enough after shot 7 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.06 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.07 nm/sec Defocus = -1.848 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_021_12.0_Oct23_04.26.50.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 89.8 In SetExposureForMean 90, change by a factor of 1.0029 would require too small a change in exposure time Image shift -0.239 0.424 IS units -1385.1 245.9 unbinned pixels; need stage -0.042 -0.509 if reset Tilting to -32 -32.00 degrees 0.2 -5.3 unbinned pixels; 1.8 0.6 nm along two shift axes; 1.9 0.2 nm on specimen axes Rate = 9.37393 A/sec -0.0 2.0 unbinned pixels; 0.7 0.2 nm along two shift axes; -0.7 -0.1 nm on specimen axes Rate = 3.53695 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.02 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = 0.077 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_022_-32.0_Oct23_04.27.57.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 82.6 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.067 0.021 IS units -121.2 161.8 unbinned pixels; need stage -0.052 -0.057 if reset Tilting to -34 -1.0 -3.7 unbinned pixels; 1.1 0.8 nm along two shift axes; 1.3 -0.2 nm on specimen axes Rate = 6.81465 A/sec Drift is low enough after shot 1 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.04 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.02 nm/sec Defocus = 0.134 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_023_-34.0_Oct23_04.28.55.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 86.4 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.032 -0.049 IS units 100.9 133.8 unbinned pixels; need stage -0.049 0.038 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -7.0 3.7 unbinned pixels; 2.0 1.9 nm along two shift axes; -1.1 -2.6 nm on specimen axes Rate = 13.90199 A/sec -4.3 2.3 unbinned pixels; 1.3 1.1 nm along two shift axes; -0.7 -1.6 nm on specimen axes Rate = 8.51818 A/sec -3.1 2.0 unbinned pixels; 1.0 0.8 nm along two shift axes; -0.6 -1.1 nm on specimen axes Rate = 6.38898 A/sec Drift is low enough after shot 3 Measured defocus = -4.12 microns changed by -0.13 to target drift = 0.09 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -1.971 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_024_14.0_Oct23_04.30.11.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 102.0 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.253 0.455 IS units -1482.1 255.9 unbinned pixels; need stage -0.042 -0.549 if reset Tilting to 16 Correcting tilt backlash -10.4 -3.1 unbinned pixels; 0.2 3.8 nm along two shift axes; 1.4 -3.6 nm on specimen axes Rate = 19.1535 A/sec -3.1 4.8 unbinned pixels; 1.9 0.5 nm along two shift axes; -1.6 -1.2 nm on specimen axes Rate = 9.98076 A/sec -2.0 1.8 unbinned pixels; 0.8 0.5 nm along two shift axes; -0.6 -0.8 nm on specimen axes Rate = 4.80681 A/sec Drift is low enough after shot 3 Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.08 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -2.040 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_025_16.0_Oct23_04.31.26.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 87.0 In SetExposureForMean 90, change by a factor of 1.0348 would require too small a change in exposure time Image shift -0.216 0.392 IS units -1275.4 213.4 unbinned pixels; need stage -0.034 -0.476 if reset Tilting to -36 -36.00 degrees 0.0 -7.3 unbinned pixels; 2.4 0.9 nm along two shift axes; 2.6 0.2 nm on specimen axes Rate = 12.95168 A/sec 0.8 2.2 unbinned pixels; 0.6 0.5 nm along two shift axes; -0.8 0.2 nm on specimen axes Rate = 4.15619 A/sec Drift is low enough after shot 2 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.02 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.01 nm/sec Defocus = 0.219 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_026_-36.0_Oct23_04.32.35.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 78.5 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift 0.038 -0.198 IS units 574.8 84.4 unbinned pixels; need stage -0.047 0.248 if reset Tilting to -38 1.8 -1.9 unbinned pixels; 0.8 0.4 nm along two shift axes; 0.6 0.7 nm on specimen axes Rate = 4.58911 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.06 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = 0.294 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_027_-38.0_Oct23_04.33.33.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 87.9 In SetExposureForMean 90, change by a factor of 1.0253 would require too small a change in exposure time Image shift 0.065 -0.254 IS units 752.2 64.0 unbinned pixels; need stage -0.045 0.335 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -13.2 4.6 unbinned pixels; 3.1 3.8 nm along two shift axes; -1.2 -4.8 nm on specimen axes Rate = 24.47335 A/sec -6.8 2.7 unbinned pixels; 1.7 1.9 nm along two shift axes; -0.7 -2.5 nm on specimen axes Rate = 12.75225 A/sec -4.4 1.7 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.5 -1.6 nm on specimen axes Rate = 8.27831 A/sec -3.9 1.6 unbinned pixels; 1.0 1.1 nm along two shift axes; -0.4 -1.4 nm on specimen axes Rate = 7.44649 A/sec Drift is low enough after shot 4 Measured defocus = -4.14 microns changed by -0.11 to target drift = 0.07 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.156 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_028_18.0_Oct23_04.34.54.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 98.5 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.221 0.406 IS units -1316.6 214.5 unbinned pixels; need stage -0.033 -0.497 if reset Tilting to 20 Correcting tilt backlash -3.5 -1.3 unbinned pixels; 0.0 1.3 nm along two shift axes; 0.6 -1.2 nm on specimen axes Rate = 6.65243 A/sec Drift is low enough after shot 1 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.15 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.09 nm/sec Defocus = -2.233 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_029_20.0_Oct23_04.35.54.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 90.1 In SetExposureForMean 90, change by a factor of 0.9978 would require too small a change in exposure time Image shift -0.180 0.319 IS units -1040.9 186.6 unbinned pixels; need stage -0.032 -0.398 if reset Tilting to -40 -40.00 degrees 0.7 -6.7 unbinned pixels; 2.3 0.6 nm along two shift axes; 2.3 0.5 nm on specimen axes Rate = 11.82892 A/sec 2.6 2.4 unbinned pixels; 0.5 1.2 nm along two shift axes; -0.9 0.8 nm on specimen axes Rate = 6.30982 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.04 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = 0.365 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_030_-40.0_Oct23_04.37.03.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 79.4 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift 0.127 -0.399 IS units 1207.1 33.9 unbinned pixels; need stage -0.049 0.556 if reset Tilting to -42 -0.8 -3.6 unbinned pixels; 1.1 0.7 nm along two shift axes; 1.3 -0.2 nm on specimen axes Rate = 6.51485 A/sec Drift is low enough after shot 1 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.07 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = 0.424 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_031_-42.0_Oct23_04.38.01.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 87.6 In SetExposureForMean 90, change by a factor of 1.0293 would require too small a change in exposure time Image shift 0.157 -0.459 IS units 1397.4 9.0 unbinned pixels; need stage -0.046 0.664 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -19.9 7.3 unbinned pixels; 4.7 5.7 nm along two shift axes; -2.0 -7.2 nm on specimen axes Rate = 37.07899 A/sec -9.2 4.1 unbinned pixels; 2.4 2.5 nm along two shift axes; -1.2 -3.3 nm on specimen axes Rate = 17.61165 A/sec -7.3 2.3 unbinned pixels; 1.6 2.2 nm along two shift axes; -0.6 -2.6 nm on specimen axes Rate = 13.49952 A/sec -6.2 1.9 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.2 nm on specimen axes Rate = 11.3435 A/sec -3.9 2.2 unbinned pixels; 1.2 1.0 nm along two shift axes; -0.7 -1.4 nm on specimen axes Rate = 7.89241 A/sec Drift is low enough after shot 5 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.12 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.347 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_032_22.0_Oct23_04.39.30.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 100.5 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.208 0.369 IS units -1204.2 214.7 unbinned pixels; need stage -0.037 -0.467 if reset Tilting to 24 Correcting tilt backlash -5.9 -1.1 unbinned pixels; 0.3 2.1 nm along two shift axes; 0.6 -2.1 nm on specimen axes Rate = 10.63373 A/sec -3.0 6.5 unbinned pixels; 2.5 0.2 nm along two shift axes; -2.2 -1.2 nm on specimen axes Rate = 12.59993 A/sec -1.5 2.9 unbinned pixels; 1.1 0.1 nm along two shift axes; -1.0 -0.6 nm on specimen axes Rate = 5.73774 A/sec Drift is low enough after shot 3 Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.09 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.04 nm/sec Defocus = -2.366 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_033_24.0_Oct23_04.40.41.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 86.0 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.165 0.250 IS units -840.4 210.1 unbinned pixels; need stage -0.046 -0.333 if reset Tilting to -44 -44.00 degrees 2.0 -6.7 unbinned pixels; 2.4 0.1 nm along two shift axes; 2.3 0.9 nm on specimen axes Rate = 12.20913 A/sec 2.0 3.3 unbinned pixels; 0.9 1.0 nm along two shift axes; -1.2 0.6 nm on specimen axes Rate = 6.76803 A/sec Drift is low enough after shot 2 Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.04 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = 0.464 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_034_-44.0_Oct23_04.41.50.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 79.3 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift 0.224 -0.621 IS units 1901.8 -17.7 unbinned pixels; need stage -0.052 0.936 if reset Tilting to -46 -0.7 -1.7 unbinned pixels; 0.5 0.5 nm along two shift axes; 0.6 -0.2 nm on specimen axes Rate = 3.3379 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.07 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.03 nm/sec Defocus = 0.525 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_035_-46.0_Oct23_04.42.48.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 86.0 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift 0.252 -0.675 IS units 2078.1 -43.0 unbinned pixels; need stage -0.049 1.060 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -15.3 6.5 unbinned pixels; 3.9 4.3 nm along two shift axes; -1.8 -5.6 nm on specimen axes Rate = 29.19256 A/sec -8.7 2.8 unbinned pixels; 1.9 2.6 nm along two shift axes; -0.7 -3.1 nm on specimen axes Rate = 16.00965 A/sec -5.5 2.3 unbinned pixels; 1.4 1.5 nm along two shift axes; -0.6 -2.0 nm on specimen axes Rate = 10.38172 A/sec -4.5 1.8 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.5 -1.6 nm on specimen axes Rate = 8.49164 A/sec -3.4 1.2 unbinned pixels; 0.8 1.0 nm along two shift axes; -0.3 -1.2 nm on specimen axes Rate = 6.26856 A/sec Drift is low enough after shot 5 Measured defocus = -4.14 microns changed by -0.11 to target drift = 0.12 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -2.461 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_036_26.0_Oct23_04.44.16.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 107.7 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.190 0.294 IS units -984.2 236.9 unbinned pixels; need stage -0.051 -0.396 if reset Tilting to 28 Correcting tilt backlash -5.8 -0.9 unbinned pixels; 0.4 2.0 nm along two shift axes; 0.5 -2.0 nm on specimen axes Rate = 10.29208 A/sec -4.9 6.6 unbinned pixels; 2.8 0.8 nm along two shift axes; -2.2 -1.9 nm on specimen axes Rate = 14.41394 A/sec -0.6 1.9 unbinned pixels; 0.7 0.0 nm along two shift axes; -0.6 -0.3 nm on specimen axes Rate = 3.44942 A/sec Drift is low enough after shot 3 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -2.472 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_037_28.0_Oct23_04.45.28.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 87.1 In SetExposureForMean 90, change by a factor of 1.0330 would require too small a change in exposure time Image shift -0.140 0.176 IS units -616.0 213.3 unbinned pixels; need stage -0.054 -0.255 if reset Tilting to -48 -48.00 degrees 0.7 -4.7 unbinned pixels; 1.6 0.3 nm along two shift axes; 1.6 0.4 nm on specimen axes Rate = 8.30809 A/sec 2.3 2.0 unbinned pixels; 0.4 1.0 nm along two shift axes; -0.8 0.8 nm on specimen axes Rate = 5.38288 A/sec Drift is low enough after shot 2 Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.03 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = 0.546 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_038_-48.0_Oct23_04.46.38.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 72.5 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift 0.305 -0.811 IS units 2498.6 -59.1 unbinned pixels; need stage -0.057 1.323 if reset Tilting to -50 2.8 -0.2 unbinned pixels; 0.4 0.9 nm along two shift axes; -0.0 1.0 nm on specimen axes Rate = 4.85823 A/sec Drift is low enough after shot 1 Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.03 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = 0.567 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_039_-50.0_Oct23_04.47.36.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 87.1 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift 0.327 -0.852 IS units 2629.8 -78.6 unbinned pixels; need stage -0.054 1.451 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -14.9 5.8 unbinned pixels; 3.6 4.2 nm along two shift axes; -1.6 -5.4 nm on specimen axes Rate = 27.94599 A/sec -7.2 2.5 unbinned pixels; 1.7 2.1 nm along two shift axes; -0.7 -2.6 nm on specimen axes Rate = 13.33993 A/sec -6.5 2.7 unbinned pixels; 1.6 1.8 nm along two shift axes; -0.7 -2.4 nm on specimen axes Rate = 12.28045 A/sec -4.9 -0.1 unbinned pixels; 0.5 1.6 nm along two shift axes; 0.2 -1.7 nm on specimen axes Rate = 8.6863 A/sec -4.9 2.4 unbinned pixels; 1.4 1.3 nm along two shift axes; -0.7 -1.8 nm on specimen axes Rate = 9.4671 A/sec -3.3 0.6 unbinned pixels; 0.6 1.0 nm along two shift axes; -0.1 -1.2 nm on specimen axes Rate = 5.84867 A/sec Drift is low enough after shot 6 Measured defocus = -4.12 microns changed by -0.13 to target drift = 0.08 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -2.606 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_040_30.0_Oct23_04.49.10.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 112.3 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.171 0.237 IS units -809.6 239.3 unbinned pixels; need stage -0.057 -0.339 if reset Tilting to 32 Correcting tilt backlash -7.3 0.3 unbinned pixels; 0.9 2.4 nm along two shift axes; 0.1 -2.6 nm on specimen axes Rate = 12.83216 A/sec -3.4 6.4 unbinned pixels; 2.5 0.4 nm along two shift axes; -2.1 -1.4 nm on specimen axes Rate = 12.78363 A/sec -0.9 3.3 unbinned pixels; 1.2 0.1 nm along two shift axes; -1.1 -0.4 nm on specimen axes Rate = 6.0703 A/sec Drift is low enough after shot 3 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.06 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.07 nm/sec Defocus = -2.613 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_041_32.0_Oct23_04.50.23.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 86.7 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift -0.128 0.144 IS units -516.9 212.1 unbinned pixels; need stage -0.057 -0.224 if reset Tilting to -52 -52.00 degrees 1.3 -3.3 unbinned pixels; 1.3 0.0 nm along two shift axes; 1.1 0.6 nm on specimen axes Rate = 6.28223 A/sec Drift is low enough after shot 1 Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.06 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = 0.615 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_042_-52.0_Oct23_04.51.27.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 73.5 In SetExposureForMean 90, exposure time changed to 3.3600 Image shift 0.368 -0.954 IS units 2948.6 -94.8 unbinned pixels; need stage -0.058 1.698 if reset Tilting to -54 -0.2 -4.3 unbinned pixels; 1.4 0.6 nm along two shift axes; 1.5 0.1 nm on specimen axes Rate = 7.54544 A/sec Drift is low enough after shot 1 Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.00 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.01 nm/sec Defocus = 0.629 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_043_-54.0_Oct23_04.52.25.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 85.0 In SetExposureForMean 90, exposure time changed to 3.5200 Image shift 0.399 -1.004 IS units 3112.1 -130.6 unbinned pixels; need stage -0.051 1.879 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -13.8 4.4 unbinned pixels; 3.1 4.1 nm along two shift axes; -1.1 -5.0 nm on specimen axes Rate = 25.39617 A/sec -8.4 2.8 unbinned pixels; 1.9 2.4 nm along two shift axes; -0.7 -3.0 nm on specimen axes Rate = 15.48764 A/sec -6.7 3.2 unbinned pixels; 1.9 1.8 nm along two shift axes; -0.9 -2.5 nm on specimen axes Rate = 13.0581 A/sec -5.5 1.8 unbinned pixels; 1.2 1.6 nm along two shift axes; -0.4 -2.0 nm on specimen axes Rate = 10.19836 A/sec -3.1 0.9 unbinned pixels; 0.6 0.9 nm along two shift axes; -0.2 -1.1 nm on specimen axes Rate = 5.65485 A/sec Drift is low enough after shot 5 Measured defocus = -4.11 microns changed by -0.14 to target drift = 0.09 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -2.764 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_044_34.0_Oct23_04.53.55.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift -0.144 0.192 IS units -663.1 210.1 unbinned pixels; need stage -0.052 -0.291 if reset Tilting to 36 Correcting tilt backlash -8.5 1.2 unbinned pixels; 1.4 2.7 nm along two shift axes; -0.2 -3.0 nm on specimen axes Rate = 14.98411 A/sec -3.5 6.9 unbinned pixels; 2.7 0.3 nm along two shift axes; -2.3 -1.4 nm on specimen axes Rate = 13.658 A/sec 0.8 3.6 unbinned pixels; 1.1 0.7 nm along two shift axes; -1.3 0.2 nm on specimen axes Rate = 6.44508 A/sec Drift is low enough after shot 3 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.10 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -2.794 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_045_36.0_Oct23_04.55.08.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 84.7 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift -0.114 0.130 IS units -465.0 186.7 unbinned pixels; need stage -0.050 -0.211 if reset Tilting to -56 -56.00 degrees 0.1 1.3 unbinned pixels; 0.4 0.2 nm along two shift axes; -0.5 -0.0 nm on specimen axes Rate = 2.26865 A/sec Drift is low enough after shot 1 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.07 nm/sec Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.08 nm/sec Defocus = 0.671 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_046_-56.0_Oct23_04.56.14.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 67.8 In SetExposureForMean 90, exposure time changed to 3.8400 Image shift 0.430 -1.079 IS units 3348.3 -144.2 unbinned pixels; need stage -0.053 2.126 if reset Tilting to -58 5.1 -7.3 unbinned pixels; 3.0 0.8 nm along two shift axes; 2.4 2.0 nm on specimen axes Rate = 15.56806 A/sec -2.8 5.5 unbinned pixels; 2.1 0.3 nm along two shift axes; -1.8 -1.2 nm on specimen axes Rate = 10.80959 A/sec -0.3 0.4 unbinned pixels; 0.2 0.0 nm along two shift axes; -0.1 -0.1 nm on specimen axes Rate = 0.81678 A/sec Drift is low enough after shot 3 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.03 nm/sec Measured defocus = -4.36 microns changed by 0.11 to target drift = 0.01 nm/sec Defocus = 0.681 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_047_-58.0_Oct23_04.57.25.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 83.3 In SetExposureForMean 90, exposure time changed to 4.1600 Image shift 0.466 -1.152 IS units 3578.8 -172.0 unbinned pixels; need stage -0.051 2.398 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -13.1 2.3 unbinned pixels; 2.3 4.1 nm along two shift axes; -0.4 -4.7 nm on specimen axes Rate = 23.32491 A/sec -6.0 3.2 unbinned pixels; 1.8 1.6 nm along two shift axes; -0.9 -2.2 nm on specimen axes Rate = 11.92808 A/sec -8.9 3.0 unbinned pixels; 2.0 2.6 nm along two shift axes; -0.8 -3.2 nm on specimen axes Rate = 16.44528 A/sec -5.6 2.4 unbinned pixels; 1.5 1.5 nm along two shift axes; -0.7 -2.0 nm on specimen axes Rate = 10.64026 A/sec -4.0 2.3 unbinned pixels; 1.2 1.1 nm along two shift axes; -0.7 -1.5 nm on specimen axes Rate = 8.12218 A/sec -4.1 1.6 unbinned pixels; 1.0 1.2 nm along two shift axes; -0.4 -1.5 nm on specimen axes Rate = 7.68312 A/sec Drift is low enough after shot 6 Measured defocus = -4.07 microns changed by -0.18 to target drift = 0.08 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.09 nm/sec Defocus = -2.959 um Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_048_38.0_Oct23_04.59.06.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 123.8 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift -0.137 0.165 IS units -580.9 215.5 unbinned pixels; need stage -0.056 -0.270 if reset Tilting to 40 Correcting tilt backlash -10.1 0.6 unbinned pixels; 1.4 3.3 nm along two shift axes; 0.1 -3.6 nm on specimen axes Rate = 17.71001 A/sec -2.0 6.3 unbinned pixels; 2.3 0.1 nm along two shift axes; -2.2 -0.9 nm on specimen axes Rate = 11.62589 A/sec -5.1 5.6 unbinned pixels; 2.5 1.0 nm along two shift axes; -1.8 -2.0 nm on specimen axes Rate = 13.27837 A/sec 3.1 2.0 unbinned pixels; 0.3 1.3 nm along two shift axes; -0.8 1.0 nm on specimen axes Rate = 6.51906 A/sec Drift is low enough after shot 4 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.07 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.06 nm/sec Defocus = -3.013 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_049_40.0_Oct23_05.00.25.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 85.3 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift -0.104 0.105 IS units -385.7 183.4 unbinned pixels; need stage -0.051 -0.186 if reset Tilting to -60 -60.00 degrees 4.1 -5.3 unbinned pixels; 2.2 0.7 nm along two shift axes; 1.7 1.6 nm on specimen axes Rate = 11.75806 A/sec 2.7 1.8 unbinned pixels; 0.3 1.1 nm along two shift axes; -0.7 0.9 nm on specimen axes Rate = 5.65757 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.03 nm/sec Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.03 nm/sec Defocus = 0.663 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_050_-60.0_Oct23_05.01.37.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 63.2 In SetExposureForMean 90, exposure time changed to 4.6400 Image shift 0.511 -1.257 IS units 3908.9 -196.3 unbinned pixels; need stage -0.053 2.777 if reset Tilting to -62 0.8 -0.3 unbinned pixels; 0.2 0.2 nm along two shift axes; 0.1 0.3 nm on specimen axes Rate = 1.53197 A/sec Drift is low enough after shot 1 Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.04 nm/sec Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.07 nm/sec Defocus = 0.718 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_051_-62.0_Oct23_05.02.37.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 82.1 In SetExposureForMean 90, exposure time changed to 5.1200 Trimming A by 20 25, reference by 20 20 Image shift 0.546 -1.333 IS units 4149.6 -217.9 unbinned pixels; need stage -0.053 3.140 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -12.8 2.8 unbinned pixels; 2.4 3.9 nm along two shift axes; -0.6 -4.6 nm on specimen axes Rate = 22.9766 A/sec -10.5 3.0 unbinned pixels; 2.2 3.1 nm along two shift axes; -0.7 -3.8 nm on specimen axes Rate = 19.14271 A/sec -8.3 2.6 unbinned pixels; 1.8 2.4 nm along two shift axes; -0.7 -3.0 nm on specimen axes Rate = 15.192 A/sec -3.0 2.9 unbinned pixels; 1.3 0.7 nm along two shift axes; -0.9 -1.1 nm on specimen axes Rate = 7.30837 A/sec Drift is low enough after shot 4 Measured defocus = -4.08 microns changed by -0.17 to target drift = 0.14 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.11 nm/sec Defocus = -3.199 um Dewars are NOT busy filling 32 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_052_42.0_Oct23_05.04.03.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 135.1 In SetExposureForMean 90, exposure time changed to 3.3600 Image shift -0.108 0.100 IS units -376.8 198.8 unbinned pixels; need stage -0.057 -0.188 if reset Tilting to 44 Correcting tilt backlash -9.8 0.1 unbinned pixels; 1.2 3.2 nm along two shift axes; 0.3 -3.4 nm on specimen axes Rate = 17.20937 A/sec -1.4 6.7 unbinned pixels; 2.4 0.3 nm along two shift axes; -2.3 -0.7 nm on specimen axes Rate = 12.03173 A/sec 0.8 3.3 unbinned pixels; 1.0 0.7 nm along two shift axes; -1.2 0.2 nm on specimen axes Rate = 5.92842 A/sec Drift is low enough after shot 3 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.09 nm/sec Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.09 nm/sec Defocus = -3.206 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_053_44.0_Oct23_05.05.18.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 82.5 In SetExposureForMean 90, exposure time changed to 3.6800 Image shift -0.067 0.024 IS units -131.0 160.1 unbinned pixels; need stage -0.051 -0.072 if reset Tilting to -64 -64.00 degrees 1.8 -5.4 unbinned pixels; 2.0 0.1 nm along two shift axes; 1.9 0.8 nm on specimen axes Rate = 10.05886 A/sec 0.0 5.7 unbinned pixels; 1.9 0.7 nm along two shift axes; -2.0 -0.2 nm on specimen axes Rate = 10.02703 A/sec 5.6 -2.8 unbinned pixels; 1.6 1.5 nm along two shift axes; 0.8 2.0 nm on specimen axes Rate = 10.94065 A/sec -1.5 0.5 unbinned pixels; 0.3 0.4 nm along two shift axes; -0.1 -0.5 nm on specimen axes Rate = 2.76746 A/sec Drift is low enough after shot 4 Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.09 nm/sec Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.10 nm/sec Defocus = 0.738 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_054_-64.0_Oct23_05.06.42.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 57.0 In SetExposureForMean 90, exposure time changed to 5.6000 Trimming A by 20 45, reference by 30 35 Image shift 0.593 -1.432 IS units 4463.6 -252.0 unbinned pixels; need stage -0.051 3.619 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash -13.6 2.6 unbinned pixels; 2.5 4.2 nm along two shift axes; -0.5 -4.8 nm on specimen axes Rate = 24.24173 A/sec -7.2 6.2 unbinned pixels; 2.9 1.6 nm along two shift axes; -2.0 -2.7 nm on specimen axes Rate = 16.61418 A/sec -4.4 1.3 unbinned pixels; 0.9 1.3 nm along two shift axes; -0.3 -1.6 nm on specimen axes Rate = 8.08986 A/sec -4.1 3.0 unbinned pixels; 1.5 1.0 nm along two shift axes; -0.9 -1.5 nm on specimen axes Rate = 8.87085 A/sec -3.6 1.1 unbinned pixels; 0.8 1.0 nm along two shift axes; -0.3 -1.3 nm on specimen axes Rate = 6.4895 A/sec Drift is low enough after shot 5 Measured defocus = -4.05 microns changed by -0.20 to target drift = 0.12 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -3.421 um Dewars are NOT busy filling 35 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_055_46.0_Oct23_05.08.14.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 127.8 In SetExposureForMean 90, exposure time changed to 4.0000 Image shift -0.068 -0.000 IS units -65.9 185.1 unbinned pixels; need stage -0.062 -0.042 if reset Tilting to 48 Correcting tilt backlash -9.0 -0.9 unbinned pixels; 0.7 3.1 nm along two shift axes; 0.6 -3.1 nm on specimen axes Rate = 15.92904 A/sec 1.1 7.5 unbinned pixels; 2.4 1.2 nm along two shift axes; -2.7 0.1 nm on specimen axes Rate = 13.33727 A/sec -1.9 2.1 unbinned pixels; 0.9 0.4 nm along two shift axes; -0.7 -0.7 nm on specimen axes Rate = 4.97414 A/sec Drift is low enough after shot 3 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.03 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -3.475 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_08_16_056_48.0_Oct23_05.09.29.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 83.5 In SetExposureForMean 90, exposure time changed to 4.3200 Image shift -0.018 -0.084 IS units 210.1 129.3 unbinned pixels; need stage -0.051 0.105 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -3.55 microns changed by -0.70 to target drift = 0.13 nm/sec Focus area changed from stored state, axis position -2.70 angle 0, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_09.st Running script 1 at item 18 (119.35, -132.04) 05:10:24 Refine ZLP: additional offset = -0.1 eV; total offset = 7.2 eV Item 18: Stage: 119.35 -132.04 -235 Label: 18 Note: Sec 16 - anchor_maps.map Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Target defocus is: -4.25 um Target defocus is: -4.50 um Target defocus = -4.5 Angle = -4.40, increment = 0.60, shift = -10.4 -4.3 pixels, -0.025 um Y Trimming A by 40 40, reference by 20 20 Angle = -3.20, increment = 1.80, shift = -30.0 -7.8 pixels, -0.070 um Y Angle = 0.41, increment = 5.41, shift = -126.2 -20.7 pixels, -0.292 um Y Trimming A by 20 25, reference by 20 20 Angle = 3.00, increment = 8.00, shift = -177.3 -29.2 pixels, -0.410 um Y Rough eucentricity: changing Z by 2.94 to -232.02, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 20 30, reference by 20 30 Angle = 18.00, shift = -62.7, 1.5 pixels, 0.019, -0.142 um, IS = -0.019, 0.142 Trimming A by 28 20, reference by 20 30 Angle = 11.00, shift = -38.7, 0.2 pixels, 0.010, -0.088 um, IS = -0.030, 0.230 Trimming A by 20 20, reference by 23 20 Angle = 4.00, shift = -45.5, -0.6 pixels, 0.010, -0.103 um, IS = -0.040, 0.333 Trimming A by 25 20 20 20, reference by 20 20 20 20 Angle = -4.00, shift = -5.7, -16.9 pixels, -0.037, -0.017 um, IS = -0.003, 0.350 Trimming A by 20 20, reference by 35 35 Angle = -11.00, shift = -59.4, -10.5 pixels, -0.009, -0.137 um, IS = 0.006, 0.488 Trimming A by 20 20, reference by 40 40 Angle = -18.00, shift = -31.6, 0.5 pixels, 0.009, -0.072 um, IS = -0.004, 0.560 Angle = -24.00, shift = 15.4, 8.4 pixels, 0.015, 0.037 um, IS = -0.019, 0.523 General solution delZ 0.65, delY 0.63, yZero 0.37 solution with assumed YZero 0.34: delZ 0.65, delY 0.27 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.65 microns and lateral displacement of -0.63 microns Disparity in stage position after aligning to center of map frame: -0.07 -0.53 Aligning to target with image shift equivalent to stage shift: -0.07 -0.54 Disparity in stage position after aligning to map item itself: -0.07 -0.49 (align shift 0.00 0.01) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 119.36 -132.01 -232.65 -1.1 1.1 unbinned pixels; 0.5 0.2 nm along two shift axes; -0.3 -0.4 nm on specimen axes Rate = 2.66746 A/sec Drift is low enough after shot 1 Measured defocus = -3.50 microns changed by -1.00 to target drift = 0.04 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -1.028 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_000_-10.0_Oct23_05.18.26.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 78.5 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.276 0.426 IS units -1425.9 347.5 unbinned pixels; need stage -0.076 -0.523 if reset Tilting to -8 Correcting tilt backlash 9.5 0.5 unbinned pixels; 0.9 3.2 nm along two shift axes; -0.5 3.3 nm on specimen axes Rate = 16.78497 A/sec 5.0 0.8 unbinned pixels; 0.3 1.7 nm along two shift axes; -0.4 1.7 nm on specimen axes Rate = 8.82373 A/sec 2.1 0.2 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.1 0.7 nm on specimen axes Rate = 3.75142 A/sec Drift is low enough after shot 3 Measured defocus = -4.37 microns changed by -0.13 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.163 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_001_-8.0_Oct23_05.19.36.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 86.6 In SetExposureForMean 90, change by a factor of 1.0389 would require too small a change in exposure time Image shift -0.318 0.442 IS units -1509.8 444.5 unbinned pixels; need stage -0.107 -0.554 if reset Tilting to -12 -12.00 degrees Trimming A by 20 25, reference by 20 20 -4.7 6.1 unbinned pixels; 2.6 0.8 nm along two shift axes; -2.0 -1.8 nm on specimen axes Rate = 13.42505 A/sec -1.8 3.2 unbinned pixels; 1.3 0.2 nm along two shift axes; -1.1 -0.7 nm on specimen axes Rate = 6.44 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.992 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_002_-12.0_Oct23_05.20.40.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 85.9 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.375 0.557 IS units -1878.1 490.1 unbinned pixels; need stage -0.111 -0.695 if reset Tilting to -14 Trimming A by 20 25, reference by 20 20 -0.7 2.4 unbinned pixels; 0.9 0.1 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.33427 A/sec Drift is low enough after shot 1 Measured defocus = -4.61 microns changed by 0.11 to target drift = 0.02 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.01 nm/sec Defocus = -0.875 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_003_-14.0_Oct23_05.21.38.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 93.3 In SetExposureForMean 90, change by a factor of 0.9653 would require too small a change in exposure time Image shift -0.424 0.659 IS units -2203.1 527.4 unbinned pixels; need stage -0.114 -0.820 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees Trimming A by 20 25, reference by 20 20 6.7 -6.8 unbinned pixels; 3.0 1.4 nm along two shift axes; 2.2 2.5 nm on specimen axes Rate = 16.73841 A/sec Trimming A by 20 25, reference by 20 20 8.0 1.8 unbinned pixels; 0.3 2.9 nm along two shift axes; -0.9 2.8 nm on specimen axes Rate = 14.4219 A/sec Trimming A by 20 25, reference by 20 20 3.6 0.7 unbinned pixels; 0.2 1.3 nm along two shift axes; -0.3 1.2 nm on specimen axes Rate = 6.37255 A/sec Drift is low enough after shot 3 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -1.265 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_004_-6.0_Oct23_05.22.55.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 94.7 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.283 0.365 IS units -1269.0 423.6 unbinned pixels; need stage -0.107 -0.465 if reset Tilting to -4 Correcting tilt backlash Trimming A by 20 20, reference by 20 25 Trimming A by 20 20, reference by 20 25 -4.4 -5.1 unbinned pixels; 1.2 2.1 nm along two shift axes; 1.9 -1.4 nm on specimen axes Rate = 11.88701 A/sec Trimming A by 20 20, reference by 20 25 1.0 2.2 unbinned pixels; 0.6 0.6 nm along two shift axes; -0.8 0.3 nm on specimen axes Rate = 4.28111 A/sec Drift is low enough after shot 2 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.01 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.01 nm/sec Defocus = -1.403 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_005_-4.0_Oct23_05.24.01.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 86.9 In SetExposureForMean 90, change by a factor of 1.0362 would require too small a change in exposure time Image shift -0.250 0.306 IS units -1076.5 390.2 unbinned pixels; need stage -0.101 -0.394 if reset Tilting to -16 -16.00 degrees Trimming A by 20 20, reference by 20 25 Trimming A by 20 25, reference by 20 25 -6.0 -4.4 unbinned pixels; 0.8 2.5 nm along two shift axes; 1.7 -2.0 nm on specimen axes Rate = 13.07268 A/sec Trimming A by 20 25, reference by 20 25 -1.4 2.1 unbinned pixels; 0.9 0.2 nm along two shift axes; -0.7 -0.6 nm on specimen axes Rate = 4.52622 A/sec Drift is low enough after shot 2 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.04 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.801 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_006_-16.0_Oct23_05.25.06.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 84.7 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.390 0.568 IS units -1923.8 522.2 unbinned pixels; need stage -0.120 -0.725 if reset Tilting to -18 Trimming A by 20 35, reference by 25 25 Trimming A by 25 25, reference by 25 25 4.0 -0.3 unbinned pixels; 0.5 1.3 nm along two shift axes; -0.0 1.4 nm on specimen axes Rate = 6.95129 A/sec Drift is low enough after shot 1 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.706 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_007_-18.0_Oct23_05.26.04.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 91.8 In SetExposureForMean 90, change by a factor of 0.9815 would require too small a change in exposure time Image shift -0.384 0.566 IS units -1910.9 506.6 unbinned pixels; need stage -0.116 -0.728 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees Trimming A by 25 25, reference by 20 20 8.5 0.5 unbinned pixels; 0.8 2.9 nm along two shift axes; -0.4 3.0 nm on specimen axes Rate = 15.03084 A/sec 5.4 1.3 unbinned pixels; 0.2 1.9 nm along two shift axes; -0.6 1.9 nm on specimen axes Rate = 9.76489 A/sec 2.8 0.6 unbinned pixels; 0.1 1.0 nm along two shift axes; -0.3 1.0 nm on specimen axes Rate = 4.99101 A/sec Drift is low enough after shot 3 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.06 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -1.480 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_008_-2.0_Oct23_05.27.17.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 94.4 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.286 0.363 IS units -1265.5 434.0 unbinned pixels; need stage -0.110 -0.462 if reset Tilting to 0 Correcting tilt backlash -5.7 0.6 unbinned pixels; 0.9 1.8 nm along two shift axes; -0.0 -2.0 nm on specimen axes Rate = 10.04922 A/sec -3.2 0.4 unbinned pixels; 0.5 1.0 nm along two shift axes; -0.0 -1.1 nm on specimen axes Rate = 5.70589 A/sec Drift is low enough after shot 2 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -1.580 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_009_-0.0_Oct23_05.28.22.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 86.4 In SetExposureForMean 90, change by a factor of 1.0412 would require too small a change in exposure time Image shift -0.276 0.352 IS units -1225.0 415.2 unbinned pixels; need stage -0.105 -0.447 if reset Tilting to -20 -20.00 degrees Trimming A by 20 20, reference by 25 30 Trimming A by 30 30, reference by 25 30 -2.7 -5.4 unbinned pixels; 1.5 1.5 nm along two shift axes; 2.0 -0.8 nm on specimen axes Rate = 10.62789 A/sec Trimming A by 30 30, reference by 25 30 -1.2 2.0 unbinned pixels; 0.8 0.2 nm along two shift axes; -0.6 -0.5 nm on specimen axes Rate = 4.0451 A/sec Drift is low enough after shot 2 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.02 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.651 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_010_-20.0_Oct23_05.29.34.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 83.1 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.341 0.469 IS units -1606.2 482.6 unbinned pixels; need stage -0.117 -0.621 if reset Tilting to -22 Trimming A by 25 30, reference by 30 30 Trimming A by 20 40, reference by 30 30 1.3 -2.4 unbinned pixels; 0.9 0.1 nm along two shift axes; 0.8 0.5 nm on specimen axes Rate = 4.75909 A/sec Drift is low enough after shot 1 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.04 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.551 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_011_-22.0_Oct23_05.30.31.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 89.8 In SetExposureForMean 90, change by a factor of 1.0035 would require too small a change in exposure time Image shift -0.319 0.430 IS units -1478.5 460.4 unbinned pixels; need stage -0.113 -0.580 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 7.4 0.4 unbinned pixels; 0.7 2.5 nm along two shift axes; -0.4 2.6 nm on specimen axes Rate = 12.93592 A/sec 5.3 1.9 unbinned pixels; 0.0 2.0 nm along two shift axes; -0.8 1.8 nm on specimen axes Rate = 9.96161 A/sec 1.6 0.8 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 3.19985 A/sec Drift is low enough after shot 3 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -1.666 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_012_2.0_Oct23_05.31.45.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 94.0 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.315 0.410 IS units -1421.6 466.7 unbinned pixels; need stage -0.117 -0.518 if reset Tilting to 4 Correcting tilt backlash -15.0 2.5 unbinned pixels; 2.6 4.7 nm along two shift axes; -0.4 -5.3 nm on specimen axes Rate = 26.72706 A/sec -10.0 2.1 unbinned pixels; 1.9 3.1 nm along two shift axes; -0.4 -3.6 nm on specimen axes Rate = 17.95651 A/sec -7.4 1.6 unbinned pixels; 1.4 2.3 nm along two shift axes; -0.4 -2.7 nm on specimen axes Rate = 13.34533 A/sec -6.3 1.4 unbinned pixels; 1.2 1.9 nm along two shift axes; -0.3 -2.3 nm on specimen axes Rate = 11.3915 A/sec -6.3 0.6 unbinned pixels; 0.9 2.0 nm along two shift axes; -0.0 -2.2 nm on specimen axes Rate = 11.10894 A/sec -2.6 1.4 unbinned pixels; 0.8 0.7 nm along two shift axes; -0.4 -0.9 nm on specimen axes Rate = 5.11288 A/sec Drift is low enough after shot 6 Measured defocus = -4.39 microns changed by -0.11 to target drift = 0.09 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.09 nm/sec Defocus = -1.779 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_013_4.0_Oct23_05.33.13.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 85.9 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.329 0.445 IS units -1528.7 471.6 unbinned pixels; need stage -0.115 -0.557 if reset Tilting to -24 -24.00 degrees Trimming A by 20 25, reference by 20 40 Trimming A by 20 40, reference by 20 40 -1.7 -4.3 unbinned pixels; 1.2 1.1 nm along two shift axes; 1.6 -0.5 nm on specimen axes Rate = 8.24873 A/sec Trimming A by 20 40, reference by 20 40 0.1 1.9 unbinned pixels; 0.6 0.3 nm along two shift axes; -0.7 -0.0 nm on specimen axes Rate = 3.31554 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.01 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.516 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_014_-24.0_Oct23_05.34.20.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 88.5 In SetExposureForMean 90, change by a factor of 1.0190 would require too small a change in exposure time Image shift -0.285 0.340 IS units -1200.9 451.9 unbinned pixels; need stage -0.119 -0.481 if reset Tilting to -26 Trimming A by 25 35, reference by 20 40 Trimming A by 20 35, reference by 20 40 1.7 -1.7 unbinned pixels; 0.8 0.4 nm along two shift axes; 0.5 0.6 nm on specimen axes Rate = 4.17627 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.451 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_015_-26.0_Oct23_05.35.18.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 87.2 In SetExposureForMean 90, change by a factor of 1.0335 would require too small a change in exposure time Image shift -0.266 0.304 IS units -1086.2 433.6 unbinned pixels; need stage -0.116 -0.443 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 4.7 1.2 unbinned pixels; 0.1 1.7 nm along two shift axes; -0.6 1.6 nm on specimen axes Rate = 8.60793 A/sec 2.2 1.2 unbinned pixels; 0.1 0.9 nm along two shift axes; -0.5 0.7 nm on specimen axes Rate = 4.46568 A/sec Drift is low enough after shot 2 Measured defocus = -4.37 microns changed by -0.13 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.910 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_016_6.0_Oct23_05.36.26.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 93.2 In SetExposureForMean 90, change by a factor of 0.9662 would require too small a change in exposure time Image shift -0.343 0.448 IS units -1552.1 507.9 unbinned pixels; need stage -0.127 -0.568 if reset Tilting to 8 Correcting tilt backlash -24.1 6.2 unbinned pixels; 4.9 7.2 nm along two shift axes; -1.4 -8.6 nm on specimen axes Rate = 43.53578 A/sec -16.2 4.6 unbinned pixels; 3.4 4.8 nm along two shift axes; -1.1 -5.8 nm on specimen axes Rate = 29.49731 A/sec -11.4 2.8 unbinned pixels; 2.3 3.4 nm along two shift axes; -0.6 -4.1 nm on specimen axes Rate = 20.501 A/sec -7.8 2.3 unbinned pixels; 1.7 2.3 nm along two shift axes; -0.6 -2.8 nm on specimen axes Rate = 14.26247 A/sec -6.8 2.0 unbinned pixels; 1.5 2.0 nm along two shift axes; -0.5 -2.4 nm on specimen axes Rate = 12.38112 A/sec -5.3 1.6 unbinned pixels; 1.2 1.5 nm along two shift axes; -0.4 -1.9 nm on specimen axes Rate = 9.65623 A/sec -4.3 1.3 unbinned pixels; 0.9 1.3 nm along two shift axes; -0.3 -1.6 nm on specimen axes Rate = 7.95038 A/sec Drift is low enough after shot 7 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.09 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.032 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_017_8.0_Oct23_05.37.59.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 92.3 In SetExposureForMean 90, change by a factor of 0.9751 would require too small a change in exposure time Image shift -0.355 0.473 IS units -1630.3 517.2 unbinned pixels; need stage -0.128 -0.599 if reset Tilting to -28 -28.00 degrees Trimming A by 25 30, reference by 20 35 Trimming A by 20 40, reference by 20 35 -1.5 -5.1 unbinned pixels; 1.5 1.1 nm along two shift axes; 1.8 -0.4 nm on specimen axes Rate = 9.35391 A/sec Trimming A by 20 20, reference by 20 35 1.7 2.5 unbinned pixels; 0.6 0.9 nm along two shift axes; -0.9 0.5 nm on specimen axes Rate = 5.3364 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.02 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = -0.388 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_018_-28.0_Oct23_05.39.07.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 85.9 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.237 0.217 IS units -821.5 439.8 unbinned pixels; need stage -0.126 -0.345 if reset Tilting to -30 Trimming A by 20 45, reference by 20 20 -0.1 -5.5 unbinned pixels; 1.8 0.7 nm along two shift axes; 1.9 0.1 nm on specimen axes Rate = 9.78773 A/sec 3.8 2.9 unbinned pixels; 0.5 1.6 nm along two shift axes; -1.1 1.2 nm on specimen axes Rate = 8.34294 A/sec 1.9 1.0 unbinned pixels; 0.1 0.7 nm along two shift axes; -0.4 0.6 nm on specimen axes Rate = 3.75897 A/sec Drift is low enough after shot 3 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.03 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.327 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_019_-30.0_Oct23_05.40.17.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 91.2 In SetExposureForMean 90, change by a factor of 0.9885 would require too small a change in exposure time Image shift -0.205 0.146 IS units -597.1 418.0 unbinned pixels; need stage -0.125 -0.259 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -8.5 5.3 unbinned pixels; 2.8 2.2 nm along two shift axes; -1.6 -3.1 nm on specimen axes Rate = 17.55638 A/sec -5.8 2.7 unbinned pixels; 1.6 1.6 nm along two shift axes; -0.8 -2.1 nm on specimen axes Rate = 11.20675 A/sec -4.0 2.6 unbinned pixels; 1.3 1.0 nm along two shift axes; -0.8 -1.5 nm on specimen axes Rate = 8.4617 A/sec -3.0 1.6 unbinned pixels; 0.9 0.8 nm along two shift axes; -0.5 -1.1 nm on specimen axes Rate = 5.92923 A/sec Drift is low enough after shot 4 Measured defocus = -4.34 microns changed by -0.16 to target drift = 0.06 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -2.172 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_020_10.0_Oct23_05.41.42.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 99.2 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.377 0.481 IS units -1675.4 568.5 unbinned pixels; need stage -0.144 -0.620 if reset Tilting to 12 Correcting tilt backlash -7.0 -0.3 unbinned pixels; 0.7 2.4 nm along two shift axes; 0.3 -2.4 nm on specimen axes Rate = 12.28004 A/sec -2.5 4.6 unbinned pixels; 1.8 0.3 nm along two shift axes; -1.5 -1.0 nm on specimen axes Rate = 9.24659 A/sec -3.5 0.9 unbinned pixels; 0.7 1.0 nm along two shift axes; -0.2 -1.3 nm on specimen axes Rate = 6.34801 A/sec Drift is low enough after shot 3 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.276 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_021_12.0_Oct23_05.42.53.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 90.8 In SetExposureForMean 90, change by a factor of 0.9913 would require too small a change in exposure time Image shift -0.357 0.450 IS units -1569.9 543.5 unbinned pixels; need stage -0.139 -0.585 if reset Tilting to -32 -32.00 degrees Trimming A by 25 25, reference by 20 20 -1.2 -4.7 unbinned pixels; 1.4 1.0 nm along two shift axes; 1.7 -0.3 nm on specimen axes Rate = 8.54424 A/sec 0.9 3.3 unbinned pixels; 1.0 0.7 nm along two shift axes; -1.2 0.2 nm on specimen axes Rate = 5.99692 A/sec Drift is low enough after shot 2 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.04 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.03 nm/sec Defocus = -0.276 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_022_-32.0_Oct23_05.44.00.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 82.6 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.165 0.009 IS units -187.4 439.9 unbinned pixels; need stage -0.146 -0.095 if reset Tilting to -34 2.3 -1.5 unbinned pixels; 0.8 0.6 nm along two shift axes; 0.5 0.9 nm on specimen axes Rate = 4.85386 A/sec Drift is low enough after shot 1 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.09 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -0.205 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_023_-34.0_Oct23_05.44.57.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 87.5 In SetExposureForMean 90, change by a factor of 1.0320 would require too small a change in exposure time Image shift -0.149 -0.025 IS units -78.4 430.6 unbinned pixels; need stage -0.146 -0.050 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -17.2 3.9 unbinned pixels; 3.3 5.2 nm along two shift axes; -0.8 -6.2 nm on specimen axes Rate = 30.96539 A/sec -9.7 2.9 unbinned pixels; 2.1 2.9 nm along two shift axes; -0.7 -3.5 nm on specimen axes Rate = 17.82158 A/sec -11.2 3.0 unbinned pixels; 2.3 3.4 nm along two shift axes; -0.7 -4.0 nm on specimen axes Rate = 20.32074 A/sec -4.3 1.4 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.3 -1.6 nm on specimen axes Rate = 7.92682 A/sec Drift is low enough after shot 4 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.11 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -2.415 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_024_14.0_Oct23_05.46.25.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 97.1 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.376 0.461 IS units -1619.5 584.8 unbinned pixels; need stage -0.151 -0.610 if reset Tilting to 16 Correcting tilt backlash -10.3 0.9 unbinned pixels; 1.5 3.3 nm along two shift axes; 0.0 -3.6 nm on specimen axes Rate = 18.18285 A/sec -7.0 3.4 unbinned pixels; 1.9 1.9 nm along two shift axes; -1.0 -2.5 nm on specimen axes Rate = 13.55229 A/sec -6.3 1.9 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.3 nm on specimen axes Rate = 11.52041 A/sec -4.5 1.2 unbinned pixels; 0.9 1.3 nm along two shift axes; -0.3 -1.6 nm on specimen axes Rate = 8.09329 A/sec -4.1 1.9 unbinned pixels; 1.1 1.1 nm along two shift axes; -0.5 -1.5 nm on specimen axes Rate = 7.83832 A/sec Drift is low enough after shot 5 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.09 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -2.513 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_025_16.0_Oct23_05.47.48.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 88.5 In SetExposureForMean 90, change by a factor of 1.0176 would require too small a change in exposure time Image shift -0.370 0.437 IS units -1549.1 590.8 unbinned pixels; need stage -0.156 -0.590 if reset Tilting to -36 -36.00 degrees Trimming A by 20 25, reference by 20 20 4.1 -6.6 unbinned pixels; 2.7 0.6 nm along two shift axes; 2.2 1.6 nm on specimen axes Rate = 13.56335 A/sec 2.6 2.4 unbinned pixels; 0.5 1.1 nm along two shift axes; -0.9 0.8 nm on specimen axes Rate = 6.17587 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.172 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_026_-36.0_Oct23_05.48.56.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 78.6 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.093 -0.194 IS units 434.2 439.5 unbinned pixels; need stage -0.165 0.172 if reset Tilting to -38 -2.8 -5.1 unbinned pixels; 1.4 1.5 nm along two shift axes; 1.9 -0.8 nm on specimen axes Rate = 10.27696 A/sec 1.5 2.6 unbinned pixels; 0.7 0.8 nm along two shift axes; -1.0 0.5 nm on specimen axes Rate = 5.32198 A/sec Drift is low enough after shot 2 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.150 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_027_-38.0_Oct23_05.49.59.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 89.3 In SetExposureForMean 90, change by a factor of 1.0121 would require too small a change in exposure time Image shift -0.059 -0.261 IS units 649.4 409.9 unbinned pixels; need stage -0.161 0.275 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -9.1 -1.1 unbinned pixels; 0.7 3.1 nm along two shift axes; 0.7 -3.2 nm on specimen axes Rate = 16.13937 A/sec -4.5 3.9 unbinned pixels; 1.8 1.0 nm along two shift axes; -1.2 -1.7 nm on specimen axes Rate = 10.45395 A/sec -3.0 2.0 unbinned pixels; 1.0 0.8 nm along two shift axes; -0.6 -1.1 nm on specimen axes Rate = 6.36279 A/sec Drift is low enough after shot 3 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.06 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.06 nm/sec Defocus = -2.651 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_028_18.0_Oct23_05.51.14.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 101.8 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.354 0.396 IS units -1421.9 588.2 unbinned pixels; need stage -0.159 -0.549 if reset Tilting to 20 Correcting tilt backlash -4.6 -0.3 unbinned pixels; 0.4 1.6 nm along two shift axes; 0.3 -1.6 nm on specimen axes Rate = 8.17292 A/sec -1.6 5.6 unbinned pixels; 2.1 0.1 nm along two shift axes; -1.9 -0.7 nm on specimen axes Rate = 10.28435 A/sec -1.4 2.7 unbinned pixels; 1.0 0.2 nm along two shift axes; -0.9 -0.6 nm on specimen axes Rate = 5.29883 A/sec Drift is low enough after shot 3 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.07 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -2.696 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_029_20.0_Oct23_05.52.25.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 85.8 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.320 0.312 IS units -1159.8 574.2 unbinned pixels; need stage -0.162 -0.456 if reset Tilting to -40 -40.00 degrees Trimming A by 20 40, reference by 20 20 0.1 -5.7 unbinned pixels; 1.9 0.7 nm along two shift axes; 2.0 0.2 nm on specimen axes Rate = 9.98147 A/sec 0.7 2.5 unbinned pixels; 0.8 0.5 nm along two shift axes; -0.9 0.2 nm on specimen axes Rate = 4.6273 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.00 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.073 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_030_-40.0_Oct23_05.53.39.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 80.4 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.011 -0.396 IS units 1062.4 408.5 unbinned pixels; need stage -0.173 0.473 if reset Tilting to -42 2.3 -5.7 unbinned pixels; 2.2 0.1 nm along two shift axes; 1.9 1.0 nm on specimen axes Rate = 10.82821 A/sec 2.8 2.1 unbinned pixels; 0.4 1.2 nm along two shift axes; -0.8 0.9 nm on specimen axes Rate = 6.19392 A/sec Drift is low enough after shot 2 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = -0.025 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_031_-42.0_Oct23_05.54.41.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 89.5 In SetExposureForMean 90, change by a factor of 1.0112 would require too small a change in exposure time Image shift 0.020 -0.457 IS units 1258.2 380.7 unbinned pixels; need stage -0.170 0.582 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -13.1 3.4 unbinned pixels; 2.6 3.9 nm along two shift axes; -0.8 -4.7 nm on specimen axes Rate = 23.65121 A/sec -7.9 4.6 unbinned pixels; 2.5 2.1 nm along two shift axes; -1.4 -2.9 nm on specimen axes Rate = 16.08752 A/sec -6.9 1.8 unbinned pixels; 1.4 2.1 nm along two shift axes; -0.4 -2.5 nm on specimen axes Rate = 12.44772 A/sec -5.3 1.6 unbinned pixels; 1.1 1.6 nm along two shift axes; -0.4 -1.9 nm on specimen axes Rate = 9.77487 A/sec -4.7 1.1 unbinned pixels; 0.9 1.4 nm along two shift axes; -0.2 -1.7 nm on specimen axes Rate = 8.49584 A/sec -2.8 0.9 unbinned pixels; 0.6 0.8 nm along two shift axes; -0.2 -1.0 nm on specimen axes Rate = 5.0882 A/sec Drift is low enough after shot 6 Measured defocus = -4.35 microns changed by -0.15 to target drift = 0.11 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.845 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_032_22.0_Oct23_05.56.14.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 105.1 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.374 0.421 IS units -1510.5 618.4 unbinned pixels; need stage -0.166 -0.598 if reset Tilting to 24 Correcting tilt backlash -8.0 -2.3 unbinned pixels; 0.2 2.9 nm along two shift axes; 1.1 -2.7 nm on specimen axes Rate = 14.61598 A/sec -2.2 5.5 unbinned pixels; 2.1 0.1 nm along two shift axes; -1.9 -0.9 nm on specimen axes Rate = 10.35838 A/sec -0.5 2.6 unbinned pixels; 0.9 0.1 nm along two shift axes; -0.9 -0.3 nm on specimen axes Rate = 4.65935 A/sec Drift is low enough after shot 3 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.07 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -2.875 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_033_24.0_Oct23_05.57.26.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 89.5 In SetExposureForMean 90, change by a factor of 1.0077 would require too small a change in exposure time Image shift -0.318 0.280 IS units -1071.3 600.7 unbinned pixels; need stage -0.174 -0.436 if reset Tilting to -44 -44.00 degrees 2.3 -7.0 unbinned pixels; 2.6 0.1 nm along two shift axes; 2.4 1.0 nm on specimen axes Rate = 12.97944 A/sec 0.6 2.4 unbinned pixels; 0.7 0.5 nm along two shift axes; -0.9 0.1 nm on specimen axes Rate = 4.34985 A/sec Drift is low enough after shot 2 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.04 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = 0.025 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_034_-44.0_Oct23_05.58.34.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 74.8 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift 0.077 -0.592 IS units 1679.3 353.6 unbinned pixels; need stage -0.173 0.810 if reset Tilting to -46 -0.2 -5.8 unbinned pixels; 1.9 0.8 nm along two shift axes; 2.0 0.1 nm on specimen axes Rate = 10.29514 A/sec 3.9 3.4 unbinned pixels; 0.7 1.7 nm along two shift axes; -1.3 1.3 nm on specimen axes Rate = 9.13233 A/sec 1.0 0.8 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.3 0.3 nm on specimen axes Rate = 2.1854 A/sec Drift is low enough after shot 3 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.02 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = 0.028 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_035_-46.0_Oct23_05.59.44.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 88.6 In SetExposureForMean 90, change by a factor of 1.0238 would require too small a change in exposure time Image shift 0.111 -0.667 IS units 1915.7 331.4 unbinned pixels; need stage -0.173 0.960 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -17.9 5.7 unbinned pixels; 4.0 5.2 nm along two shift axes; -1.5 -6.4 nm on specimen axes Rate = 32.85985 A/sec -8.7 3.3 unbinned pixels; 2.1 2.5 nm along two shift axes; -0.9 -3.2 nm on specimen axes Rate = 16.34172 A/sec -7.5 2.3 unbinned pixels; 1.6 2.2 nm along two shift axes; -0.6 -2.7 nm on specimen axes Rate = 13.80938 A/sec -5.2 2.1 unbinned pixels; 1.3 1.5 nm along two shift axes; -0.6 -1.9 nm on specimen axes Rate = 9.82039 A/sec -4.6 1.5 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.38905 A/sec -4.5 -0.4 unbinned pixels; 0.4 1.5 nm along two shift axes; 0.3 -1.6 nm on specimen axes Rate = 7.91291 A/sec Drift is low enough after shot 6 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.11 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -3.021 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_036_26.0_Oct23_06.01.19.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 107.6 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.352 0.365 IS units -1335.3 610.8 unbinned pixels; need stage -0.169 -0.547 if reset Tilting to 28 Correcting tilt backlash -6.4 -2.6 unbinned pixels; 0.1 2.4 nm along two shift axes; 1.1 -2.2 nm on specimen axes Rate = 12.2593 A/sec 0.3 5.5 unbinned pixels; 1.8 0.7 nm along two shift axes; -1.9 -0.1 nm on specimen axes Rate = 9.77535 A/sec -0.6 2.5 unbinned pixels; 0.9 0.1 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.44857 A/sec Drift is low enough after shot 3 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -3.056 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_037_28.0_Oct23_06.02.31.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 85.5 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.294 0.220 IS units -886.4 590.2 unbinned pixels; need stage -0.176 -0.377 if reset Tilting to -48 -48.00 degrees 2.2 -2.0 unbinned pixels; 0.9 0.5 nm along two shift axes; 0.6 0.8 nm on specimen axes Rate = 5.21853 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.08 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.08 nm/sec Defocus = 0.103 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_038_-48.0_Oct23_06.03.34.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 74.0 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift 0.134 -0.736 IS units 2126.3 335.1 unbinned pixels; need stage -0.181 1.107 if reset Tilting to -50 -0.4 -1.0 unbinned pixels; 0.3 0.3 nm along two shift axes; 0.3 -0.1 nm on specimen axes Rate = 1.84632 A/sec Drift is low enough after shot 1 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = 0.064 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_039_-50.0_Oct23_06.04.31.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 85.3 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift 0.156 -0.778 IS units 2261.8 314.7 unbinned pixels; need stage -0.178 1.228 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -18.3 3.8 unbinned pixels; 3.4 5.6 nm along two shift axes; -0.8 -6.5 nm on specimen axes Rate = 32.82603 A/sec -8.9 4.3 unbinned pixels; 2.5 2.4 nm along two shift axes; -1.3 -3.2 nm on specimen axes Rate = 17.32528 A/sec -6.9 2.1 unbinned pixels; 1.5 2.0 nm along two shift axes; -0.5 -2.5 nm on specimen axes Rate = 12.6199 A/sec -5.8 2.1 unbinned pixels; 1.4 1.7 nm along two shift axes; -0.6 -2.1 nm on specimen axes Rate = 10.73408 A/sec -4.8 2.0 unbinned pixels; 1.2 1.3 nm along two shift axes; -0.6 -1.7 nm on specimen axes Rate = 9.10078 A/sec -3.3 0.8 unbinned pixels; 0.6 1.0 nm along two shift axes; -0.2 -1.2 nm on specimen axes Rate = 5.94897 A/sec Drift is low enough after shot 6 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.10 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -3.190 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_040_30.0_Oct23_06.06.06.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 114.8 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.321 0.286 IS units -1090.3 601.2 unbinned pixels; need stage -0.173 -0.468 if reset Tilting to 32 Correcting tilt backlash -6.0 -5.3 unbinned pixels; 1.1 2.6 nm along two shift axes; 2.0 -1.9 nm on specimen axes Rate = 14.15922 A/sec -2.5 4.8 unbinned pixels; 1.9 0.3 nm along two shift axes; -1.6 -1.0 nm on specimen axes Rate = 9.49054 A/sec -1.2 2.0 unbinned pixels; 0.8 0.2 nm along two shift axes; -0.7 -0.5 nm on specimen axes Rate = 4.11377 A/sec Drift is low enough after shot 3 Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.07 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -3.193 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_041_32.0_Oct23_06.07.19.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 86.9 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.265 0.146 IS units -657.7 583.2 unbinned pixels; need stage -0.180 -0.296 if reset Tilting to -52 -52.00 degrees 2.3 -6.0 unbinned pixels; 2.3 0.0 nm along two shift axes; 2.0 1.0 nm on specimen axes Rate = 11.31104 A/sec 4.2 3.0 unbinned pixels; 0.5 1.7 nm along two shift axes; -1.2 1.4 nm on specimen axes Rate = 9.10336 A/sec 2.8 1.2 unbinned pixels; 0.1 1.1 nm along two shift axes; -0.5 1.0 nm on specimen axes Rate = 5.42972 A/sec Drift is low enough after shot 3 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.05 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.05 nm/sec Defocus = 0.101 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_042_-52.0_Oct23_06.08.35.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 71.2 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift 0.186 -0.869 IS units 2538.1 321.3 unbinned pixels; need stage -0.189 1.441 if reset Tilting to -54 -0.3 -6.3 unbinned pixels; 2.1 0.8 nm along two shift axes; 2.2 0.1 nm on specimen axes Rate = 11.20991 A/sec 0.7 3.6 unbinned pixels; 1.1 0.7 nm along two shift axes; -1.3 0.1 nm on specimen axes Rate = 6.48232 A/sec Drift is low enough after shot 2 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = 0.136 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_043_-54.0_Oct23_06.09.39.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 84.6 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift 0.224 -0.941 IS units 2769.0 285.3 unbinned pixels; need stage -0.183 1.650 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -20.5 4.7 unbinned pixels; 3.9 6.2 nm along two shift axes; -1.0 -7.3 nm on specimen axes Rate = 36.9012 A/sec -8.9 3.2 unbinned pixels; 2.1 2.6 nm along two shift axes; -0.8 -3.2 nm on specimen axes Rate = 16.59172 A/sec -8.3 2.5 unbinned pixels; 1.8 2.4 nm along two shift axes; -0.6 -3.0 nm on specimen axes Rate = 15.17921 A/sec -7.2 1.9 unbinned pixels; 1.5 2.1 nm along two shift axes; -0.4 -2.6 nm on specimen axes Rate = 12.97161 A/sec -4.2 1.7 unbinned pixels; 1.0 1.2 nm along two shift axes; -0.5 -1.5 nm on specimen axes Rate = 7.9114 A/sec Drift is low enough after shot 5 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.15 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.10 nm/sec Defocus = -3.336 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_044_34.0_Oct23_06.11.09.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 119.6 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.292 0.207 IS units -848.0 597.4 unbinned pixels; need stage -0.179 -0.385 if reset Tilting to 36 Correcting tilt backlash -5.3 -2.1 unbinned pixels; 0.1 2.0 nm along two shift axes; 0.9 -1.8 nm on specimen axes Rate = 10.10612 A/sec 0.1 4.7 unbinned pixels; 1.6 0.6 nm along two shift axes; -1.7 -0.1 nm on specimen axes Rate = 8.34518 A/sec -0.9 2.3 unbinned pixels; 0.9 0.0 nm along two shift axes; -0.8 -0.4 nm on specimen axes Rate = 4.27841 A/sec Drift is low enough after shot 3 Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.09 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -3.345 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_045_36.0_Oct23_06.12.22.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 86.3 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.243 0.090 IS units -482.4 575.0 unbinned pixels; need stage -0.183 -0.234 if reset Tilting to -56 -56.00 degrees 0.3 -5.8 unbinned pixels; 2.0 0.6 nm along two shift axes; 2.0 0.3 nm on specimen axes Rate = 10.23059 A/sec 5.3 2.7 unbinned pixels; 0.3 2.1 nm along two shift axes; -1.1 1.8 nm on specimen axes Rate = 10.46747 A/sec 1.1 0.9 unbinned pixels; 0.2 0.5 nm along two shift axes; -0.4 0.4 nm on specimen axes Rate = 2.6125 A/sec Drift is low enough after shot 3 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.05 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.01 nm/sec Defocus = 0.159 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_046_-56.0_Oct23_06.13.37.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 66.4 In SetExposureForMean 90, exposure time changed to 3.5200 Image shift 0.258 -1.029 IS units 3042.6 277.6 unbinned pixels; need stage -0.189 1.908 if reset Tilting to -58 -2.1 -5.6 unbinned pixels; 1.6 1.4 nm along two shift axes; 2.0 -0.6 nm on specimen axes Rate = 10.52509 A/sec 3.0 2.9 unbinned pixels; 0.6 1.3 nm along two shift axes; -1.1 0.9 nm on specimen axes Rate = 7.33941 A/sec Drift is low enough after shot 2 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.03 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.01 nm/sec Defocus = 0.152 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_047_-58.0_Oct23_06.14.41.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 84.4 In SetExposureForMean 90, exposure time changed to 3.8400 Image shift 0.302 -1.112 IS units 3310.2 237.4 unbinned pixels; need stage -0.184 2.194 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -16.1 1.8 unbinned pixels; 2.5 5.1 nm along two shift axes; -0.1 -5.7 nm on specimen axes Rate = 28.45112 A/sec -12.3 2.8 unbinned pixels; 2.4 3.7 nm along two shift axes; -0.6 -4.4 nm on specimen axes Rate = 22.17404 A/sec -8.2 2.0 unbinned pixels; 1.6 2.5 nm along two shift axes; -0.4 -2.9 nm on specimen axes Rate = 14.74362 A/sec -6.0 1.6 unbinned pixels; 1.2 1.8 nm along two shift axes; -0.4 -2.2 nm on specimen axes Rate = 10.92542 A/sec -4.6 1.7 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.5 -1.7 nm on specimen axes Rate = 8.5351 A/sec -6.2 -0.0 unbinned pixels; 0.7 2.1 nm along two shift axes; 0.2 -2.2 nm on specimen axes Rate = 10.91538 A/sec -1.4 1.7 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.6 -0.6 nm on specimen axes Rate = 3.92906 A/sec Drift is low enough after shot 7 Measured defocus = -4.37 microns changed by -0.13 to target drift = 0.12 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -3.469 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_048_38.0_Oct23_06.16.23.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 132.8 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift -0.257 0.124 IS units -588.8 581.8 unbinned pixels; need stage -0.182 -0.288 if reset Tilting to 40 Correcting tilt backlash -4.7 -2.7 unbinned pixels; 0.4 1.9 nm along two shift axes; 1.1 -1.6 nm on specimen axes Rate = 9.6196 A/sec 2.0 4.5 unbinned pixels; 1.3 1.2 nm along two shift axes; -1.6 0.6 nm on specimen axes Rate = 8.72822 A/sec 2.8 3.2 unbinned pixels; 0.7 1.3 nm along two shift axes; -1.2 0.9 nm on specimen axes Rate = 7.44659 A/sec Drift is low enough after shot 3 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -3.522 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_049_40.0_Oct23_06.17.37.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 84.2 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift -0.203 0.014 IS units -237.5 538.6 unbinned pixels; need stage -0.178 -0.132 if reset Tilting to -60 -60.00 degrees 15.6 3.2 unbinned pixels; 0.8 5.5 nm along two shift axes; -1.6 5.4 nm on specimen axes Rate = 28.00057 A/sec 1.6 21.9 unbinned pixels; 7.1 3.2 nm along two shift axes; -7.7 -0.1 nm on specimen axes Rate = 38.86312 A/sec -5.0 -23.3 unbinned pixels; 7.2 4.4 nm along two shift axes; 8.3 -1.0 nm on specimen axes Rate = 42.12643 A/sec 12.3 17.9 unbinned pixels; 4.5 6.2 nm along two shift axes; -6.7 3.8 nm on specimen axes Rate = 38.38644 A/sec -8.5 -12.6 unbinned pixels; 3.2 4.3 nm along two shift axes; 4.7 -2.6 nm on specimen axes Rate = 26.84306 A/sec 8.3 6.0 unbinned pixels; 1.0 3.5 nm along two shift axes; -2.4 2.7 nm on specimen axes Rate = 18.08208 A/sec -1.8 -6.5 unbinned pixels; 1.9 1.4 nm along two shift axes; 2.3 -0.4 nm on specimen axes Rate = 11.88309 A/sec -8.2 0.5 unbinned pixels; 1.1 2.6 nm along two shift axes; 0.1 -2.9 nm on specimen axes Rate = 14.33111 A/sec 8.5 8.2 unbinned pixels; 1.7 3.8 nm along two shift axes; -3.1 2.7 nm on specimen axes Rate = 20.86168 A/sec -0.8 1.0 unbinned pixels; 0.4 0.2 nm along two shift axes; -0.3 -0.3 nm on specimen axes Rate = 2.23945 A/sec Drift is low enough after shot 10 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.03 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.03 nm/sec Defocus = 0.223 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_050_-60.0_Oct23_06.19.34.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 59.2 In SetExposureForMean 90, exposure time changed to 4.1600 Image shift 0.340 -1.204 IS units 3595.7 221.0 unbinned pixels; need stage -0.187 2.528 if reset Tilting to -62 -1.1 1.5 unbinned pixels; 0.6 0.2 nm along two shift axes; -0.5 -0.4 nm on specimen axes Rate = 3.31271 A/sec Drift is low enough after shot 1 Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.04 nm/sec Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.03 nm/sec Defocus = 0.144 um Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_051_-62.0_Oct23_06.20.33.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 81.9 In SetExposureForMean 90, exposure time changed to 4.6400 Image shift 0.378 -1.273 IS units 3819.5 183.0 unbinned pixels; need stage -0.180 2.864 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -12.0 3.5 unbinned pixels; 2.6 3.6 nm along two shift axes; -0.9 -4.3 nm on specimen axes Rate = 21.94176 A/sec -10.7 3.3 unbinned pixels; 2.3 3.2 nm along two shift axes; -0.8 -3.9 nm on specimen axes Rate = 19.66569 A/sec -10.3 1.6 unbinned pixels; 1.7 3.2 nm along two shift axes; -0.2 -3.7 nm on specimen axes Rate = 18.28679 A/sec -8.5 1.7 unbinned pixels; 1.5 2.6 nm along two shift axes; -0.3 -3.0 nm on specimen axes Rate = 15.10573 A/sec -1.7 2.1 unbinned pixels; 0.9 0.3 nm along two shift axes; -0.7 -0.6 nm on specimen axes Rate = 4.68945 A/sec Drift is low enough after shot 5 Measured defocus = -4.31 microns changed by -0.19 to target drift = 0.10 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.13 nm/sec Defocus = -3.728 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_052_42.0_Oct23_06.22.05.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 145.6 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift -0.224 0.052 IS units -362.8 559.9 unbinned pixels; need stage -0.182 -0.197 if reset Tilting to 44 Correcting tilt backlash -12.6 -1.0 unbinned pixels; 1.1 4.3 nm along two shift axes; 0.7 -4.4 nm on specimen axes Rate = 22.26398 A/sec -5.8 3.9 unbinned pixels; 2.0 1.4 nm along two shift axes; -1.2 -2.1 nm on specimen axes Rate = 12.18187 A/sec -3.4 2.4 unbinned pixels; 1.2 0.9 nm along two shift axes; -0.7 -1.3 nm on specimen axes Rate = 7.3045 A/sec Drift is low enough after shot 3 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.11 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -3.749 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_053_44.0_Oct23_06.23.18.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 85.4 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift -0.176 -0.027 IS units -100.6 503.5 unbinned pixels; need stage -0.170 -0.072 if reset Tilting to -64 -64.00 degrees 20.6 -16.5 unbinned pixels; 7.9 4.8 nm along two shift axes; 5.2 7.7 nm on specimen axes Rate = 46.25001 A/sec -20.4 -0.6 unbinned pixels; 2.2 6.8 nm along two shift axes; 0.9 -7.1 nm on specimen axes Rate = 35.82542 A/sec -5.4 -19.3 unbinned pixels; 5.8 4.1 nm along two shift axes; 6.9 -1.3 nm on specimen axes Rate = 35.47194 A/sec 9.6 11.7 unbinned pixels; 2.8 4.6 nm along two shift axes; -4.4 3.0 nm on specimen axes Rate = 26.75095 A/sec 10.2 -16.9 unbinned pixels; 6.8 1.4 nm along two shift axes; 5.6 4.1 nm on specimen axes Rate = 34.63658 A/sec -9.3 -2.8 unbinned pixels; 0.2 3.4 nm along two shift axes; 1.3 -3.2 nm on specimen axes Rate = 17.01434 A/sec 22.3 1.4 unbinned pixels; 2.1 7.5 nm along two shift axes; -1.2 7.8 nm on specimen axes Rate = 39.23345 A/sec -0.3 -7.6 unbinned pixels; 2.5 1.0 nm along two shift axes; 2.7 0.1 nm on specimen axes Rate = 13.36735 A/sec 9.2 6.2 unbinned pixels; 1.0 3.8 nm along two shift axes; -2.5 3.0 nm on specimen axes Rate = 19.59971 A/sec -1.1 0.4 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.1 -0.4 nm on specimen axes Rate = 2.061 A/sec Drift is low enough after shot 10 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.03 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = 0.189 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_054_-64.0_Oct23_06.25.17.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 52.8 In SetExposureForMean 90, exposure time changed to 5.2800 Image shift 0.430 -1.371 IS units 4138.8 134.1 unbinned pixels; need stage -0.174 3.328 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash 2.5 2.6 unbinned pixels; 0.6 1.1 nm along two shift axes; -1.0 0.8 nm on specimen axes Rate = 6.29244 A/sec Drift is low enough after shot 1 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.14 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.11 nm/sec Defocus = -3.866 um Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_055_46.0_Oct23_06.26.25.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 146.5 In SetExposureForMean 90, exposure time changed to 3.2000 Image shift -0.164 -0.041 IS units -51.8 485.3 unbinned pixels; need stage -0.165 -0.049 if reset Tilting to 48 Correcting tilt backlash -8.5 -1.9 unbinned pixels; 0.4 3.0 nm along two shift axes; 0.9 -2.9 nm on specimen axes Rate = 15.30368 A/sec -6.0 6.6 unbinned pixels; 2.9 1.2 nm along two shift axes; -2.1 -2.3 nm on specimen axes Rate = 15.62815 A/sec -3.5 2.2 unbinned pixels; 1.1 0.9 nm along two shift axes; -0.7 -1.3 nm on specimen axes Rate = 7.24405 A/sec Drift is low enough after shot 3 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.09 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -3.936 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_09_18_056_48.0_Oct23_06.27.40.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 81.6 In SetExposureForMean 90, exposure time changed to 3.5200 Image shift -0.131 -0.109 IS units 165.6 459.0 unbinned pixels; need stage -0.163 0.065 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -3.35 microns changed by -1.15 to target drift = 0.09 nm/sec Focus area changed from stored state, axis position -2.50 angle 0, subarea offset 29 57 Opened new file D:\Users\Ben\201022_Jans_cells\SEM\batch_tomo_10.st Running script 1 at item 20 (9.45, 14.15) 06:28:35 Refine ZLP: additional offset = -0.2 eV; total offset = 7.0 eV Item 20: Stage: 9.45 14.15 -235 Label: 20 Note: Sec 18 - anchor_maps.map Target defocus is: -4.50 um Target defocus is: -4.75 um Target defocus = -4.75 Angle = -4.40, increment = 0.60, shift = -26.3 -7.7 pixels, -0.062 um Y Trimming A by 20 35, reference by 20 20 Angle = -3.20, increment = 1.80, shift = -56.8 -14.7 pixels, -0.133 um Y Trimming A by 20 25, reference by 20 20 Angle = 0.41, increment = 5.41, shift = -179.9 -33.4 pixels, -0.417 um Y Angle = 3.00, increment = 8.00, shift = -254.3 -45.9 pixels, -0.589 um Y Rough eucentricity: changing Z by 4.22 to -230.72, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = 7.9, 10.7 pixels, 0.022, 0.021 um, IS = -0.022, -0.021 Trimming A by 25 30, reference by 20 20 Angle = 11.00, shift = -11.8, 2.9 pixels, 0.010, -0.026 um, IS = -0.032, 0.005 Trimming A by 20 25, reference by 20 30 Angle = 4.00, shift = 11.9, 6.4 pixels, 0.011, 0.029 um, IS = -0.043, -0.023 Trimming A by 20 20 20 20, reference by 21 20 20 25 Angle = -4.00, shift = -22.9, -24.5 pixels, -0.050, -0.058 um, IS = 0.006, 0.035 Angle = -11.00, shift = -8.8, -9.1 pixels, -0.018, -0.022 um, IS = 0.025, 0.057 Angle = -18.00, shift = 45.8, 10.0 pixels, 0.011, 0.107 um, IS = 0.014, -0.049 Angle = -24.00, shift = 58.5, 13.3 pixels, 0.015, 0.136 um, IS = -0.002, -0.186 General solution delZ -0.12, delY 1.33, yZero 0.03 solution with assumed YZero 0.01: delZ -0.12, delY 0.98 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.12 microns and lateral displacement of -1.33 microns Disparity in stage position after aligning to center of map frame: -0.06 -0.99 Aligning to target with image shift equivalent to stage shift: -0.06 -0.97 Disparity in stage position after aligning to map item itself: -0.05 -0.93 (align shift 0.01 0.03) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage 9.46 14.17 -230.60 -1.9 0.0 unbinned pixels; 0.2 0.6 nm along two shift axes; 0.0 -0.7 nm on specimen axes Rate = 3.27691 A/sec Drift is low enough after shot 1 Measured defocus = -3.11 microns changed by -1.64 to target drift = 0.06 nm/sec Measured defocus = -4.70 microns changed by -0.05 to target drift = 0.06 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.691 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_000_-10.0_Oct23_06.32.25.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 91.1 In SetExposureForMean 90, change by a factor of 0.9881 would require too small a change in exposure time Image shift -0.432 0.806 IS units -2609.9 409.5 unbinned pixels; need stage -0.061 -0.951 if reset Tilting to -8 Correcting tilt backlash 9.7 -2.5 unbinned pixels; 1.9 2.9 nm along two shift axes; 0.6 3.5 nm on specimen axes Rate = 17.5585 A/sec 4.6 -1.5 unbinned pixels; 1.0 1.3 nm along two shift axes; 0.4 1.7 nm on specimen axes Rate = 8.5285 A/sec 2.1 -1.7 unbinned pixels; 0.8 0.5 nm along two shift axes; 0.5 0.8 nm on specimen axes Rate = 4.72102 A/sec Drift is low enough after shot 3 Measured defocus = -4.62 microns changed by -0.13 to target drift = 0.08 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -1.816 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_001_-8.0_Oct23_06.33.35.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 91.3 In SetExposureForMean 90, change by a factor of 0.9857 would require too small a change in exposure time Image shift -0.454 0.803 IS units -2623.8 472.7 unbinned pixels; need stage -0.082 -0.952 if reset Tilting to -12 -12.00 degrees -5.0 0.1 unbinned pixels; 0.6 1.6 nm along two shift axes; 0.1 -1.7 nm on specimen axes Rate = 8.72162 A/sec -4.9 1.4 unbinned pixels; 1.0 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 8.88789 A/sec -3.2 -1.1 unbinned pixels; 0.0 1.2 nm along two shift axes; 0.5 -1.1 nm on specimen axes Rate = 6.00467 A/sec Drift is low enough after shot 3 Measured defocus = -4.85 microns changed by 0.10 to target drift = 0.09 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -1.596 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_002_-12.0_Oct23_06.34.44.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 90.5 In SetExposureForMean 90, change by a factor of 0.9944 would require too small a change in exposure time Image shift -0.502 0.906 IS units -2949.7 505.2 unbinned pixels; need stage -0.083 -1.083 if reset Tilting to -14 -1.1 0.4 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.1 -0.4 nm on specimen axes Rate = 2.06699 A/sec Drift is low enough after shot 1 Measured defocus = -4.89 microns changed by 0.14 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -1.445 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_003_-14.0_Oct23_06.35.41.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 90.2 In SetExposureForMean 90, change by a factor of 0.9982 would require too small a change in exposure time Image shift -0.544 1.012 IS units -3277.7 518.0 unbinned pixels; need stage -0.077 -1.212 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 8.8 -8.8 unbinned pixels; 4.0 1.9 nm along two shift axes; 2.8 3.4 nm on specimen axes Rate = 21.82053 A/sec 6.4 -1.1 unbinned pixels; 1.1 2.0 nm along two shift axes; 0.2 2.3 nm on specimen axes Rate = 11.42767 A/sec 2.4 -1.6 unbinned pixels; 0.8 0.6 nm along two shift axes; 0.5 0.9 nm on specimen axes Rate = 5.05011 A/sec Drift is low enough after shot 3 Measured defocus = -4.64 microns changed by -0.11 to target drift = 0.07 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -1.932 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_004_-6.0_Oct23_06.36.58.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 91.1 In SetExposureForMean 90, change by a factor of 0.9879 would require too small a change in exposure time Image shift -0.395 0.742 IS units -2400.7 369.2 unbinned pixels; need stage -0.053 -0.866 if reset Tilting to -4 Correcting tilt backlash -6.6 -7.1 unbinned pixels; 1.6 3.0 nm along two shift axes; 2.7 -2.1 nm on specimen axes Rate = 17.0689 A/sec -0.3 0.5 unbinned pixels; 0.2 0.0 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.06506 A/sec Drift is low enough after shot 2 Measured defocus = -4.64 microns changed by -0.11 to target drift = 0.07 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -2.051 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_005_-4.0_Oct23_06.38.03.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 91.3 In SetExposureForMean 90, change by a factor of 0.9861 would require too small a change in exposure time Image shift -0.350 0.674 IS units -2171.9 312.2 unbinned pixels; need stage -0.041 -0.780 if reset Tilting to -16 -16.00 degrees -2.4 -8.7 unbinned pixels; 2.6 1.8 nm along two shift axes; 3.1 -0.6 nm on specimen axes Rate = 15.87352 A/sec -1.5 0.0 unbinned pixels; 0.2 0.5 nm along two shift axes; 0.0 -0.5 nm on specimen axes Rate = 2.70738 A/sec Drift is low enough after shot 2 Measured defocus = -4.88 microns changed by 0.13 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -1.301 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_006_-16.0_Oct23_06.39.07.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 89.9 In SetExposureForMean 90, change by a factor of 1.0012 would require too small a change in exposure time Image shift -0.481 0.933 IS units -3001.8 422.5 unbinned pixels; need stage -0.053 -1.118 if reset Tilting to -18 2.8 -0.6 unbinned pixels; 0.5 0.9 nm along two shift axes; 0.1 1.0 nm on specimen axes Rate = 5.09904 A/sec Drift is low enough after shot 1 Measured defocus = -4.85 microns changed by 0.10 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.192 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_007_-18.0_Oct23_06.40.04.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 89.1 In SetExposureForMean 90, change by a factor of 1.0098 would require too small a change in exposure time Image shift -0.477 0.941 IS units -3020.0 401.5 unbinned pixels; need stage -0.045 -1.136 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 10.9 -1.2 unbinned pixels; 1.7 3.5 nm along two shift axes; 0.1 3.9 nm on specimen axes Rate = 19.2717 A/sec 5.4 -2.0 unbinned pixels; 1.3 1.5 nm along two shift axes; 0.5 2.0 nm on specimen axes Rate = 10.11673 A/sec 2.8 -0.6 unbinned pixels; 0.5 0.8 nm along two shift axes; 0.1 1.0 nm on specimen axes Rate = 5.01285 A/sec Drift is low enough after shot 3 Measured defocus = -4.68 microns changed by -0.07 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -2.113 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_008_-2.0_Oct23_06.41.17.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 91.2 In SetExposureForMean 90, change by a factor of 0.9868 would require too small a change in exposure time Image shift -0.338 0.680 IS units -2174.9 273.0 unbinned pixels; need stage -0.027 -0.778 if reset Tilting to 0 Correcting tilt backlash -7.1 -6.4 unbinned pixels; 1.3 3.1 nm along two shift axes; 2.5 -2.3 nm on specimen axes Rate = 16.86932 A/sec -1.3 2.2 unbinned pixels; 0.9 0.2 nm along two shift axes; -0.7 -0.5 nm on specimen axes Rate = 4.45574 A/sec Drift is low enough after shot 2 Measured defocus = -4.65 microns changed by -0.10 to target drift = 0.07 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -2.215 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_009_0.0_Oct23_06.42.22.tif Saved Z = 9, 0.00 degrees Mean of buffer A = 91.1 In SetExposureForMean 90, change by a factor of 0.9876 would require too small a change in exposure time Image shift -0.301 0.624 IS units -1987.1 226.4 unbinned pixels; need stage -0.017 -0.710 if reset Tilting to -20 -20.00 degrees -1.9 -8.1 unbinned pixels; 2.5 1.6 nm along two shift axes; 2.9 -0.4 nm on specimen axes Rate = 14.67945 A/sec -0.9 1.5 unbinned pixels; 0.6 0.1 nm along two shift axes; -0.5 -0.4 nm on specimen axes Rate = 3.12635 A/sec Drift is low enough after shot 2 Measured defocus = -4.81 microns changed by 0.06 to target drift = 0.03 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -1.125 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_010_-20.0_Oct23_06.43.27.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 88.3 In SetExposureForMean 90, change by a factor of 1.0191 would require too small a change in exposure time Image shift -0.417 0.844 IS units -2698.4 333.0 unbinned pixels; need stage -0.032 -1.027 if reset Tilting to -22 5.0 -2.2 unbinned pixels; 1.3 1.4 nm along two shift axes; 0.6 1.8 nm on specimen axes Rate = 9.50336 A/sec 3.0 -0.6 unbinned pixels; 0.6 0.9 nm along two shift axes; 0.1 1.1 nm on specimen axes Rate = 5.31322 A/sec Drift is low enough after shot 2 Measured defocus = -4.88 microns changed by 0.13 to target drift = 0.06 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.994 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_011_-22.0_Oct23_06.44.30.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 87.7 In SetExposureForMean 90, change by a factor of 1.0265 would require too small a change in exposure time Image shift -0.400 0.819 IS units -2612.0 308.6 unbinned pixels; need stage -0.026 -1.007 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 8.6 -0.4 unbinned pixels; 1.1 2.8 nm along two shift axes; -0.1 3.0 nm on specimen axes Rate = 15.10776 A/sec 3.8 0.2 unbinned pixels; 0.4 1.3 nm along two shift axes; -0.2 1.3 nm on specimen axes Rate = 6.68936 A/sec Drift is low enough after shot 2 Measured defocus = -4.65 microns changed by -0.10 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -2.310 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_012_2.0_Oct23_06.45.37.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 90.8 In SetExposureForMean 90, change by a factor of 0.9916 would require too small a change in exposure time Image shift -0.296 0.625 IS units -1984.3 211.9 unbinned pixels; need stage -0.012 -0.709 if reset Tilting to 4 Correcting tilt backlash -13.8 0.2 unbinned pixels; 1.7 4.5 nm along two shift axes; 0.3 -4.8 nm on specimen axes Rate = 24.14045 A/sec -6.5 2.1 unbinned pixels; 1.4 1.9 nm along two shift axes; -0.5 -2.3 nm on specimen axes Rate = 11.88223 A/sec -7.2 1.5 unbinned pixels; 1.3 2.2 nm along two shift axes; -0.3 -2.6 nm on specimen axes Rate = 12.79653 A/sec -7.3 1.5 unbinned pixels; 1.4 2.2 nm along two shift axes; -0.3 -2.6 nm on specimen axes Rate = 13.11965 A/sec -4.3 1.3 unbinned pixels; 0.9 1.3 nm along two shift axes; -0.3 -1.5 nm on specimen axes Rate = 7.8067 A/sec Drift is low enough after shot 5 Measured defocus = -4.64 microns changed by -0.11 to target drift = 0.08 nm/sec Measured defocus = -4.73 microns changed by -0.02 to target drift = 0.10 nm/sec Defocus = -2.441 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_013_4.0_Oct23_06.47.02.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 90.4 In SetExposureForMean 90, change by a factor of 0.9960 would require too small a change in exposure time Image shift -0.286 0.611 IS units -1937.3 198.5 unbinned pixels; need stage -0.009 -0.693 if reset Tilting to -24 -24.00 degrees -0.5 -6.1 unbinned pixels; 2.0 0.9 nm along two shift axes; 2.1 0.0 nm on specimen axes Rate = 10.7793 A/sec -0.1 2.1 unbinned pixels; 0.7 0.2 nm along two shift axes; -0.8 -0.1 nm on specimen axes Rate = 3.79322 A/sec Drift is low enough after shot 2 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.00 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.913 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_014_-24.0_Oct23_06.48.09.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 86.8 In SetExposureForMean 90, change by a factor of 1.0377 would require too small a change in exposure time Image shift -0.355 0.732 IS units -2332.6 271.0 unbinned pixels; need stage -0.021 -0.912 if reset Tilting to -26 1.5 -2.1 unbinned pixels; 0.9 0.2 nm along two shift axes; 0.7 0.6 nm on specimen axes Rate = 4.60012 A/sec Drift is low enough after shot 1 Measured defocus = -4.85 microns changed by 0.10 to target drift = 0.07 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.809 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_015_-26.0_Oct23_06.49.05.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 85.8 In SetExposureForMean 90, change by a factor of 1.0495 would require too small a change in exposure time Image shift -0.335 0.693 IS units -2207.9 251.6 unbinned pixels; need stage -0.019 -0.878 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 5.6 -1.8 unbinned pixels; 1.2 1.6 nm along two shift axes; 0.5 2.0 nm on specimen axes Rate = 10.33841 A/sec 3.8 3.2 unbinned pixels; 0.6 1.6 nm along two shift axes; -1.2 1.2 nm on specimen axes Rate = 8.72296 A/sec 2.0 1.2 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.5 0.7 nm on specimen axes Rate = 4.09137 A/sec Drift is low enough after shot 3 Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -2.497 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_016_6.0_Oct23_06.50.19.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 89.8 In SetExposureForMean 90, change by a factor of 1.0024 would require too small a change in exposure time Image shift -0.267 0.596 IS units -1878.3 159.2 unbinned pixels; need stage 0.003 -0.673 if reset Tilting to 8 Correcting tilt backlash -21.7 6.9 unbinned pixels; 4.8 6.3 nm along two shift axes; -1.8 -7.8 nm on specimen axes Rate = 39.85583 A/sec -14.3 3.3 unbinned pixels; 2.8 4.3 nm along two shift axes; -0.7 -5.1 nm on specimen axes Rate = 25.68092 A/sec -12.4 2.6 unbinned pixels; 2.3 3.8 nm along two shift axes; -0.5 -4.4 nm on specimen axes Rate = 22.14589 A/sec -13.5 4.3 unbinned pixels; 3.0 4.0 nm along two shift axes; -1.1 -4.9 nm on specimen axes Rate = 24.78965 A/sec -4.8 1.6 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.8366 A/sec -4.6 1.1 unbinned pixels; 0.9 1.4 nm along two shift axes; -0.3 -1.6 nm on specimen axes Rate = 8.21738 A/sec -3.9 0.5 unbinned pixels; 0.6 1.2 nm along two shift axes; -0.0 -1.4 nm on specimen axes Rate = 6.92357 A/sec Drift is low enough after shot 7 Measured defocus = -4.64 microns changed by -0.11 to target drift = 0.11 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.616 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_017_8.0_Oct23_06.51.57.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 89.3 In SetExposureForMean 90, change by a factor of 1.0079 would require too small a change in exposure time Image shift -0.293 0.640 IS units -2021.5 188.8 unbinned pixels; need stage -0.003 -0.728 if reset Tilting to -28 -28.00 degrees -2.8 -6.0 unbinned pixels; 1.7 1.6 nm along two shift axes; 2.2 -0.8 nm on specimen axes Rate = 11.61288 A/sec 0.8 3.3 unbinned pixels; 1.0 0.6 nm along two shift axes; -1.2 0.2 nm on specimen axes Rate = 5.91667 A/sec Drift is low enough after shot 2 Measured defocus = -4.81 microns changed by 0.06 to target drift = 0.01 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.00 nm/sec Defocus = -0.738 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_018_-28.0_Oct23_06.53.03.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 84.5 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.298 0.616 IS units -1963.2 225.5 unbinned pixels; need stage -0.017 -0.794 if reset Tilting to -30 0.3 -1.7 unbinned pixels; 0.6 0.1 nm along two shift axes; 0.6 0.1 nm on specimen axes Rate = 3.05524 A/sec Drift is low enough after shot 1 Measured defocus = -4.80 microns changed by 0.05 to target drift = 0.10 nm/sec Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.04 nm/sec Defocus = -0.654 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_019_-30.0_Oct23_06.54.00.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 91.5 In SetExposureForMean 90, change by a factor of 0.9843 would require too small a change in exposure time Image shift -0.277 0.570 IS units -1816.1 210.5 unbinned pixels; need stage -0.017 -0.749 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees 5.5 1.3 unbinned pixels; 0.2 2.0 nm along two shift axes; -0.6 1.9 nm on specimen axes Rate = 9.87557 A/sec 0.4 2.1 unbinned pixels; 0.7 0.4 nm along two shift axes; -0.8 0.1 nm on specimen axes Rate = 3.85239 A/sec Drift is low enough after shot 2 Measured defocus = -4.65 microns changed by -0.10 to target drift = 0.06 nm/sec Measured defocus = -4.73 microns changed by -0.02 to target drift = 0.03 nm/sec Defocus = -2.740 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_020_10.0_Oct23_06.55.09.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 97.5 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.291 0.630 IS units -1994.7 192.0 unbinned pixels; need stage -0.005 -0.722 if reset Tilting to 12 Correcting tilt backlash -21.8 5.3 unbinned pixels; 4.3 6.6 nm along two shift axes; -1.2 -7.8 nm on specimen axes Rate = 39.27505 A/sec -14.8 4.8 unbinned pixels; 3.3 4.3 nm along two shift axes; -1.2 -5.3 nm on specimen axes Rate = 27.29205 A/sec -8.8 1.8 unbinned pixels; 1.6 2.7 nm along two shift axes; -0.4 -3.1 nm on specimen axes Rate = 15.73652 A/sec -8.0 2.3 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.6 -2.9 nm on specimen axes Rate = 14.66652 A/sec -6.0 1.6 unbinned pixels; 1.2 1.8 nm along two shift axes; -0.4 -2.1 nm on specimen axes Rate = 10.8271 A/sec -4.9 1.3 unbinned pixels; 1.0 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 8.88796 A/sec -3.8 0.4 unbinned pixels; 0.6 1.2 nm along two shift axes; -0.0 -1.3 nm on specimen axes Rate = 6.62322 A/sec Drift is low enough after shot 7 Measured defocus = -4.64 microns changed by -0.11 to target drift = 0.12 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.10 nm/sec Defocus = -2.842 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_021_12.0_Oct23_06.56.42.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 87.7 In SetExposureForMean 90, change by a factor of 1.0263 would require too small a change in exposure time Image shift -0.292 0.638 IS units -2016.5 188.9 unbinned pixels; need stage -0.003 -0.735 if reset Tilting to -32 -32.00 degrees 0.2 -5.5 unbinned pixels; 1.8 0.6 nm along two shift axes; 1.9 0.2 nm on specimen axes Rate = 9.62501 A/sec 0.7 2.2 unbinned pixels; 0.6 0.5 nm along two shift axes; -0.8 0.2 nm on specimen axes Rate = 4.08302 A/sec Drift is low enough after shot 2 Measured defocus = -4.83 microns changed by 0.08 to target drift = 0.01 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.565 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_022_-32.0_Oct23_06.57.48.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 81.9 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.229 0.461 IS units -1474.0 184.2 unbinned pixels; need stage -0.018 -0.622 if reset Tilting to -34 2.5 -0.8 unbinned pixels; 0.6 0.7 nm along two shift axes; 0.2 0.9 nm on specimen axes Rate = 4.54856 A/sec Drift is low enough after shot 1 Measured defocus = -4.81 microns changed by 0.06 to target drift = 0.03 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.497 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_023_-34.0_Oct23_06.58.50.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 88.2 In SetExposureForMean 90, change by a factor of 1.0211 would require too small a change in exposure time Image shift -0.210 0.432 IS units -1378.3 160.3 unbinned pixels; need stage -0.013 -0.594 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -9.5 -4.4 unbinned pixels; 0.3 3.7 nm along two shift axes; 1.8 -3.2 nm on specimen axes Rate = 18.40408 A/sec -7.5 5.6 unbinned pixels; 2.7 1.8 nm along two shift axes; -1.7 -2.8 nm on specimen axes Rate = 16.41604 A/sec -4.6 2.4 unbinned pixels; 1.3 1.2 nm along two shift axes; -0.7 -1.7 nm on specimen axes Rate = 9.11895 A/sec -3.1 1.1 unbinned pixels; 0.7 0.9 nm along two shift axes; -0.3 -1.1 nm on specimen axes Rate = 5.7704 A/sec Drift is low enough after shot 4 Measured defocus = -4.67 microns changed by -0.08 to target drift = 0.08 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.936 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_024_14.0_Oct23_07.00.11.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 95.4 In SetExposureForMean 90, exposure time changed to 1.6000 Image shift -0.236 0.559 IS units -1747.1 110.9 unbinned pixels; need stage 0.016 -0.640 if reset Tilting to 16 Correcting tilt backlash -18.9 4.1 unbinned pixels; 3.6 5.8 nm along two shift axes; -0.9 -6.8 nm on specimen axes Rate = 33.95252 A/sec -10.9 3.1 unbinned pixels; 2.3 3.2 nm along two shift axes; -0.7 -3.9 nm on specimen axes Rate = 19.8494 A/sec -7.6 2.7 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.7 -2.8 nm on specimen axes Rate = 14.15666 A/sec -6.6 1.6 unbinned pixels; 1.3 2.0 nm along two shift axes; -0.4 -2.4 nm on specimen axes Rate = 11.91093 A/sec -4.6 1.3 unbinned pixels; 1.0 1.4 nm along two shift axes; -0.3 -1.7 nm on specimen axes Rate = 8.42547 A/sec -3.8 0.5 unbinned pixels; 0.6 1.2 nm along two shift axes; -0.1 -1.3 nm on specimen axes Rate = 6.71277 A/sec Drift is low enough after shot 6 Measured defocus = -4.56 microns changed by -0.19 to target drift = 0.09 nm/sec Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.06 nm/sec Defocus = -3.089 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_025_16.0_Oct23_07.01.38.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 85.5 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.248 0.566 IS units -1778.4 137.6 unbinned pixels; need stage 0.007 -0.659 if reset Tilting to -36 -36.00 degrees 1.3 -4.3 unbinned pixels; 1.6 0.1 nm along two shift axes; 1.5 0.6 nm on specimen axes Rate = 7.94905 A/sec Drift is low enough after shot 1 Measured defocus = -4.82 microns changed by 0.07 to target drift = 0.06 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.408 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_026_-36.0_Oct23_07.02.39.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 86.5 In SetExposureForMean 90, change by a factor of 1.0421 would require too small a change in exposure time Image shift -0.172 0.339 IS units -1086.8 146.5 unbinned pixels; need stage -0.017 -0.481 if reset Tilting to -38 1.4 -3.6 unbinned pixels; 1.4 0.0 nm along two shift axes; 1.2 0.6 nm on specimen axes Rate = 6.76386 A/sec Drift is low enough after shot 1 Measured defocus = -4.81 microns changed by 0.06 to target drift = 0.07 nm/sec Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -0.335 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_027_-38.0_Oct23_07.03.37.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 84.5 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.146 0.287 IS units -922.8 125.2 unbinned pixels; need stage -0.015 -0.419 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -8.3 1.7 unbinned pixels; 1.5 2.5 nm along two shift axes; -0.3 -3.0 nm on specimen axes Rate = 14.84832 A/sec -6.5 2.6 unbinned pixels; 1.6 1.8 nm along two shift axes; -0.7 -2.4 nm on specimen axes Rate = 12.29256 A/sec -4.4 2.6 unbinned pixels; 1.4 1.1 nm along two shift axes; -0.8 -1.6 nm on specimen axes Rate = 8.89009 A/sec -3.4 1.0 unbinned pixels; 0.7 1.0 nm along two shift axes; -0.3 -1.2 nm on specimen axes Rate = 6.28105 A/sec Drift is low enough after shot 4 Measured defocus = -4.64 microns changed by -0.11 to target drift = 0.05 nm/sec Measured defocus = -4.73 microns changed by -0.02 to target drift = 0.07 nm/sec Defocus = -3.220 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_028_18.0_Oct23_07.04.58.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 101.5 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.259 0.559 IS units -1768.9 174.9 unbinned pixels; need stage -0.006 -0.664 if reset Tilting to 20 Correcting tilt backlash -9.0 -1.3 unbinned pixels; 0.6 3.1 nm along two shift axes; 0.7 -3.1 nm on specimen axes Rate = 15.92707 A/sec -2.2 5.8 unbinned pixels; 2.2 0.0 nm along two shift axes; -2.0 -1.0 nm on specimen axes Rate = 10.87092 A/sec -1.0 3.4 unbinned pixels; 1.2 0.1 nm along two shift axes; -1.2 -0.5 nm on specimen axes Rate = 6.24225 A/sec Drift is low enough after shot 3 Measured defocus = -4.65 microns changed by -0.10 to target drift = 0.10 nm/sec Measured defocus = -4.75 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -3.323 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_029_20.0_Oct23_07.06.08.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 91.6 In SetExposureForMean 90, change by a factor of 0.9830 would require too small a change in exposure time Image shift -0.219 0.457 IS units -1453.6 160.8 unbinned pixels; need stage -0.011 -0.553 if reset Tilting to -40 -40.00 degrees 1.6 -4.1 unbinned pixels; 1.5 0.0 nm along two shift axes; 1.4 0.7 nm on specimen axes Rate = 7.63811 A/sec Drift is low enough after shot 1 Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.08 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.293 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_030_-40.0_Oct23_07.07.11.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 82.6 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.117 0.175 IS units -589.4 151.5 unbinned pixels; need stage -0.034 -0.278 if reset Tilting to -42 3.2 -0.5 unbinned pixels; 0.6 1.0 nm along two shift axes; 0.1 1.1 nm on specimen axes Rate = 5.65828 A/sec Drift is low enough after shot 1 Measured defocus = -4.81 microns changed by 0.06 to target drift = 0.07 nm/sec Measured defocus = -4.78 microns changed by 0.03 to target drift = 0.04 nm/sec Defocus = -0.211 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_031_-42.0_Oct23_07.08.08.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 87.4 In SetExposureForMean 90, change by a factor of 1.0322 would require too small a change in exposure time Image shift -0.103 0.161 IS units -538.8 127.1 unbinned pixels; need stage -0.027 -0.262 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -4.1 1.2 unbinned pixels; 0.9 1.2 nm along two shift axes; -0.3 -1.5 nm on specimen axes Rate = 7.53425 A/sec Drift is low enough after shot 1 Measured defocus = -4.61 microns changed by -0.14 to target drift = 0.13 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -3.476 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_032_22.0_Oct23_07.09.12.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 98.3 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.202 0.466 IS units -1462.8 106.5 unbinned pixels; need stage 0.008 -0.562 if reset Tilting to 24 Correcting tilt backlash -6.7 1.3 unbinned pixels; 1.2 2.1 nm along two shift axes; -0.2 -2.4 nm on specimen axes Rate = 11.98783 A/sec -2.4 6.2 unbinned pixels; 2.3 0.0 nm along two shift axes; -2.1 -1.0 nm on specimen axes Rate = 11.73661 A/sec -0.5 3.7 unbinned pixels; 1.3 0.3 nm along two shift axes; -1.3 -0.3 nm on specimen axes Rate = 6.5458 A/sec Drift is low enough after shot 3 Measured defocus = -4.69 microns changed by -0.06 to target drift = 0.07 nm/sec Measured defocus = -4.70 microns changed by -0.05 to target drift = 0.07 nm/sec Defocus = -3.589 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_033_24.0_Oct23_07.10.22.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 88.5 In SetExposureForMean 90, change by a factor of 1.0167 would require too small a change in exposure time Image shift -0.178 0.359 IS units -1147.6 143.1 unbinned pixels; need stage -0.014 -0.449 if reset Tilting to -44 -44.00 degrees 0.4 -4.0 unbinned pixels; 1.4 0.4 nm along two shift axes; 1.4 0.2 nm on specimen axes Rate = 7.00341 A/sec Drift is low enough after shot 1 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.04 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -0.207 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_034_-44.0_Oct23_07.11.24.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 77.7 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.071 0.054 IS units -215.3 141.2 unbinned pixels; need stage -0.042 -0.112 if reset Tilting to -46 -0.5 -3.4 unbinned pixels; 1.1 0.6 nm along two shift axes; 1.2 -0.1 nm on specimen axes Rate = 6.05286 A/sec Drift is low enough after shot 1 Measured defocus = -4.83 microns changed by 0.08 to target drift = 0.06 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.118 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_035_-46.0_Oct23_07.12.22.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 89.1 In SetExposureForMean 90, change by a factor of 1.0140 would require too small a change in exposure time Image shift -0.044 0.008 IS units -64.3 112.9 unbinned pixels; need stage -0.037 -0.038 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -16.3 5.2 unbinned pixels; 3.6 4.8 nm along two shift axes; -1.3 -5.9 nm on specimen axes Rate = 29.93661 A/sec -9.2 4.2 unbinned pixels; 2.5 2.5 nm along two shift axes; -1.2 -3.4 nm on specimen axes Rate = 17.71201 A/sec -6.9 2.9 unbinned pixels; 1.8 1.9 nm along two shift axes; -0.8 -2.5 nm on specimen axes Rate = 13.09071 A/sec -6.0 2.3 unbinned pixels; 1.4 1.7 nm along two shift axes; -0.6 -2.2 nm on specimen axes Rate = 11.18562 A/sec -4.3 2.0 unbinned pixels; 1.2 1.2 nm along two shift axes; -0.6 -1.6 nm on specimen axes Rate = 8.33677 A/sec -3.5 1.5 unbinned pixels; 0.9 1.0 nm along two shift axes; -0.4 -1.3 nm on specimen axes Rate = 6.74497 A/sec Drift is low enough after shot 6 Measured defocus = -4.62 microns changed by -0.13 to target drift = 0.08 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.16 nm/sec Defocus = -3.732 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_036_26.0_Oct23_07.13.55.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 102.6 In SetExposureForMean 90, exposure time changed to 1.7600 Image shift -0.209 0.409 IS units -1314.9 178.4 unbinned pixels; need stage -0.021 -0.524 if reset Tilting to 28 Correcting tilt backlash -8.2 -2.5 unbinned pixels; 0.1 3.0 nm along two shift axes; 1.1 -2.8 nm on specimen axes Rate = 15.16694 A/sec -1.8 5.8 unbinned pixels; 2.1 0.1 nm along two shift axes; -2.0 -0.8 nm on specimen axes Rate = 10.68533 A/sec -0.9 2.4 unbinned pixels; 0.9 0.0 nm along two shift axes; -0.8 -0.4 nm on specimen axes Rate = 4.49006 A/sec Drift is low enough after shot 3 Measured defocus = -4.73 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -3.760 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_037_28.0_Oct23_07.15.07.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 84.9 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.152 0.247 IS units -817.4 178.8 unbinned pixels; need stage -0.036 -0.334 if reset Tilting to -48 -48.00 degrees 0.7 -1.4 unbinned pixels; 0.6 0.1 nm along two shift axes; 0.5 0.3 nm on specimen axes Rate = 2.83016 A/sec Drift is low enough after shot 1 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.07 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.063 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_038_-48.0_Oct23_07.16.10.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 79.1 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.044 -0.058 IS units 114.2 174.0 unbinned pixels; need stage -0.063 0.052 if reset Tilting to -50 3.1 -6.9 unbinned pixels; 2.6 0.2 nm along two shift axes; 2.3 1.3 nm on specimen axes Rate = 13.20588 A/sec 5.2 1.2 unbinned pixels; 0.2 1.9 nm along two shift axes; -0.6 1.8 nm on specimen axes Rate = 9.43303 A/sec 1.9 0.9 unbinned pixels; 0.1 0.7 nm along two shift axes; -0.4 0.6 nm on specimen axes Rate = 3.68874 A/sec Drift is low enough after shot 3 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.02 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.032 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_039_-50.0_Oct23_07.17.18.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 88.4 In SetExposureForMean 90, change by a factor of 1.0244 would require too small a change in exposure time Image shift -0.000 -0.135 IS units 365.3 128.6 unbinned pixels; need stage -0.055 0.194 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -15.9 4.5 unbinned pixels; 3.4 4.7 nm along two shift axes; -1.1 -5.7 nm on specimen axes Rate = 28.94504 A/sec -8.6 3.3 unbinned pixels; 2.1 2.4 nm along two shift axes; -0.9 -3.1 nm on specimen axes Rate = 16.09622 A/sec -8.0 2.1 unbinned pixels; 1.6 2.4 nm along two shift axes; -0.5 -2.9 nm on specimen axes Rate = 14.50684 A/sec -9.9 2.7 unbinned pixels; 2.1 3.0 nm along two shift axes; -0.6 -3.6 nm on specimen axes Rate = 18.07212 A/sec -5.3 1.3 unbinned pixels; 1.1 1.6 nm along two shift axes; -0.3 -1.9 nm on specimen axes Rate = 9.65823 A/sec -2.8 0.9 unbinned pixels; 0.6 0.8 nm along two shift axes; -0.2 -1.0 nm on specimen axes Rate = 5.22698 A/sec Drift is low enough after shot 6 Measured defocus = -4.58 microns changed by -0.17 to target drift = 0.10 nm/sec Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.09 nm/sec Defocus = -3.910 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_040_30.0_Oct23_07.18.57.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 105.3 In SetExposureForMean 90, exposure time changed to 1.9200 Image shift -0.182 0.335 IS units -1086.6 178.0 unbinned pixels; need stage -0.028 -0.450 if reset Tilting to 32 Correcting tilt backlash -9.0 -4.3 unbinned pixels; 0.4 3.5 nm along two shift axes; 1.8 -3.0 nm on specimen axes Rate = 17.64247 A/sec -5.6 7.9 unbinned pixels; 3.3 0.9 nm along two shift axes; -2.6 -2.2 nm on specimen axes Rate = 16.97121 A/sec -1.7 2.9 unbinned pixels; 1.2 0.2 nm along two shift axes; -1.0 -0.7 nm on specimen axes Rate = 5.92243 A/sec Drift is low enough after shot 3 Measured defocus = -4.70 microns changed by -0.05 to target drift = 0.06 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -3.965 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_041_32.0_Oct23_07.20.08.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 87.7 In SetExposureForMean 90, change by a factor of 1.0275 would require too small a change in exposure time Image shift -0.120 0.185 IS units -620.2 148.9 unbinned pixels; need stage -0.032 -0.264 if reset Tilting to -52 -52.00 degrees 2.9 -5.0 unbinned pixels; 2.0 0.4 nm along two shift axes; 1.7 1.2 nm on specimen axes Rate = 10.23243 A/sec 1.5 1.6 unbinned pixels; 0.3 0.7 nm along two shift axes; -0.6 0.5 nm on specimen axes Rate = 3.87703 A/sec Drift is low enough after shot 2 Measured defocus = -4.79 microns changed by 0.04 to target drift = 0.04 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = 0.017 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_042_-52.0_Oct23_07.21.17.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 72.4 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift 0.054 -0.239 IS units 700.4 80.4 unbinned pixels; need stage -0.049 0.398 if reset Tilting to -54 0.6 -6.1 unbinned pixels; 2.1 0.6 nm along two shift axes; 2.1 0.4 nm on specimen axes Rate = 10.86339 A/sec 2.6 1.6 unbinned pixels; 0.2 1.0 nm along two shift axes; -0.6 0.8 nm on specimen axes Rate = 5.33481 A/sec Drift is low enough after shot 2 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.03 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.01 nm/sec Defocus = 0.025 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_043_-54.0_Oct23_07.22.21.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 86.2 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift 0.095 -0.318 IS units 954.4 43.9 unbinned pixels; need stage -0.044 0.572 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -16.5 2.8 unbinned pixels; 2.8 5.1 nm along two shift axes; -0.5 -5.9 nm on specimen axes Rate = 29.28398 A/sec -10.3 4.4 unbinned pixels; 2.7 2.9 nm along two shift axes; -1.2 -3.7 nm on specimen axes Rate = 19.6148 A/sec -7.3 2.8 unbinned pixels; 1.8 2.1 nm along two shift axes; -0.8 -2.6 nm on specimen axes Rate = 13.66438 A/sec -6.3 2.1 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.6 -2.3 nm on specimen axes Rate = 11.73367 A/sec -4.8 2.0 unbinned pixels; 1.2 1.4 nm along two shift axes; -0.5 -1.8 nm on specimen axes Rate = 9.13111 A/sec -4.4 1.4 unbinned pixels; 1.0 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.06253 A/sec -3.4 0.8 unbinned pixels; 0.7 1.0 nm along two shift axes; -0.2 -1.2 nm on specimen axes Rate = 6.11525 A/sec Drift is low enough after shot 7 Measured defocus = -4.57 microns changed by -0.18 to target drift = 0.10 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -4.134 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_044_34.0_Oct23_07.24.07.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 113.7 In SetExposureForMean 90, exposure time changed to 2.0800 Image shift -0.131 0.234 IS units -762.5 133.5 unbinned pixels; need stage -0.022 -0.330 if reset Tilting to 36 Correcting tilt backlash -11.7 -3.7 unbinned pixels; 0.1 4.3 nm along two shift axes; 1.7 -4.0 nm on specimen axes Rate = 21.55924 A/sec -4.5 6.0 unbinned pixels; 2.5 0.8 nm along two shift axes; -2.0 -1.8 nm on specimen axes Rate = 13.12672 A/sec -1.8 2.5 unbinned pixels; 1.0 0.3 nm along two shift axes; -0.8 -0.7 nm on specimen axes Rate = 5.42366 A/sec Drift is low enough after shot 3 Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.07 nm/sec Measured defocus = -4.72 microns changed by -0.03 to target drift = 0.06 nm/sec Defocus = -4.177 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_045_36.0_Oct23_07.25.20.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 89.3 In SetExposureForMean 90, change by a factor of 1.0100 would require too small a change in exposure time Image shift -0.084 0.098 IS units -346.5 134.7 unbinned pixels; need stage -0.036 -0.157 if reset Tilting to -56 -56.00 degrees 3.7 -7.4 unbinned pixels; 2.9 0.3 nm along two shift axes; 2.5 1.5 nm on specimen axes Rate = 14.65294 A/sec 4.5 1.3 unbinned pixels; 0.1 1.6 nm along two shift axes; -0.6 1.5 nm on specimen axes Rate = 8.23026 A/sec 1.8 1.3 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.5 0.6 nm on specimen axes Rate = 3.94875 A/sec Drift is low enough after shot 3 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.05 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = 0.062 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_046_-56.0_Oct23_07.26.36.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 70.6 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift 0.157 -0.472 IS units 1432.1 21.5 unbinned pixels; need stage -0.051 0.904 if reset Tilting to -58 1.5 -6.2 unbinned pixels; 2.2 0.2 nm along two shift axes; 2.1 0.7 nm on specimen axes Rate = 11.24642 A/sec 1.1 2.4 unbinned pixels; 0.7 0.6 nm along two shift axes; -0.9 0.3 nm on specimen axes Rate = 4.6022 A/sec Drift is low enough after shot 2 Measured defocus = -4.72 microns changed by -0.03 to target drift = 0.03 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = 0.020 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_047_-58.0_Oct23_07.27.41.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 86.9 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift 0.209 -0.576 IS units 1767.4 -19.7 unbinned pixels; need stage -0.048 1.181 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -5.8 0.7 unbinned pixels; 0.9 1.8 nm along two shift axes; -0.1 -2.0 nm on specimen axes Rate = 10.18434 A/sec -5.7 5.7 unbinned pixels; 2.5 1.2 nm along two shift axes; -1.8 -2.2 nm on specimen axes Rate = 14.03933 A/sec -2.6 2.8 unbinned pixels; 1.2 0.5 nm along two shift axes; -0.9 -1.0 nm on specimen axes Rate = 6.73181 A/sec Drift is low enough after shot 3 Measured defocus = -4.63 microns changed by -0.12 to target drift = 0.12 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -4.301 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_048_38.0_Oct23_07.28.59.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 119.2 In SetExposureForMean 90, exposure time changed to 2.2400 Image shift -0.039 0.121 IS units -367.6 -8.0 unbinned pixels; need stage 0.014 -0.165 if reset Tilting to 40 Correcting tilt backlash -10.8 -3.2 unbinned pixels; 0.2 3.9 nm along two shift axes; 1.4 -3.7 nm on specimen axes Rate = 19.72698 A/sec -2.9 5.2 unbinned pixels; 2.1 0.3 nm along two shift axes; -1.7 -1.2 nm on specimen axes Rate = 10.54213 A/sec -1.2 3.0 unbinned pixels; 1.1 0.1 nm along two shift axes; -1.0 -0.5 nm on specimen axes Rate = 5.68135 A/sec Drift is low enough after shot 3 Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.09 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -4.321 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_049_40.0_Oct23_07.30.11.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 89.2 In SetExposureForMean 90, change by a factor of 1.0129 would require too small a change in exposure time Image shift -0.001 0.002 IS units -5.4 1.9 unbinned pixels; need stage -0.000 -0.003 if reset Tilting to -60 -60.00 degrees 4.2 -6.3 unbinned pixels; 2.6 0.6 nm along two shift axes; 2.1 1.7 nm on specimen axes Rate = 13.30639 A/sec 2.7 3.4 unbinned pixels; 0.8 1.3 nm along two shift axes; -1.3 0.8 nm on specimen axes Rate = 7.71053 A/sec Drift is low enough after shot 2 Measured defocus = -4.80 microns changed by 0.05 to target drift = 0.04 nm/sec Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = 0.087 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_050_-60.0_Oct23_07.31.21.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 66.7 In SetExposureForMean 90, exposure time changed to 3.0400 Image shift 0.270 -0.692 IS units 2142.1 -76.6 unbinned pixels; need stage -0.040 1.520 if reset Tilting to -62 0.5 -6.0 unbinned pixels; 2.0 0.6 nm along two shift axes; 2.1 0.4 nm on specimen axes Rate = 10.58431 A/sec 0.3 3.6 unbinned pixels; 1.2 0.6 nm along two shift axes; -1.3 0.0 nm on specimen axes Rate = 6.4701 A/sec Drift is low enough after shot 2 Measured defocus = -4.71 microns changed by -0.04 to target drift = 0.04 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = 0.036 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_051_-62.0_Oct23_07.32.24.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 83.7 In SetExposureForMean 90, exposure time changed to 3.3600 Image shift 0.318 -0.803 IS units 2489.7 -102.7 unbinned pixels; need stage -0.041 1.882 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -21.2 2.0 unbinned pixels; 3.1 6.8 nm along two shift axes; -0.1 -7.5 nm on specimen axes Rate = 37.39765 A/sec -11.4 4.4 unbinned pixels; 2.8 3.2 nm along two shift axes; -1.2 -4.1 nm on specimen axes Rate = 21.45961 A/sec -6.3 3.6 unbinned pixels; 1.9 1.6 nm along two shift axes; -1.1 -2.3 nm on specimen axes Rate = 12.65872 A/sec -6.1 2.5 unbinned pixels; 1.5 1.7 nm along two shift axes; -0.7 -2.2 nm on specimen axes Rate = 11.53192 A/sec -4.8 1.9 unbinned pixels; 1.2 1.4 nm along two shift axes; -0.5 -1.8 nm on specimen axes Rate = 9.14998 A/sec -3.6 1.2 unbinned pixels; 0.8 1.1 nm along two shift axes; -0.3 -1.3 nm on specimen axes Rate = 6.75295 A/sec Drift is low enough after shot 6 Measured defocus = -4.58 microns changed by -0.17 to target drift = 0.12 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -4.496 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_052_42.0_Oct23_07.34.02.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 128.0 In SetExposureForMean 90, exposure time changed to 2.4000 Image shift -0.020 -0.022 IS units 39.2 74.7 unbinned pixels; need stage -0.027 0.015 if reset Tilting to 44 Correcting tilt backlash -7.4 -2.9 unbinned pixels; 0.1 2.8 nm along two shift axes; 1.3 -2.5 nm on specimen axes Rate = 13.96144 A/sec -1.7 5.3 unbinned pixels; 2.0 0.1 nm along two shift axes; -1.8 -0.8 nm on specimen axes Rate = 9.76065 A/sec -0.9 2.7 unbinned pixels; 1.0 0.0 nm along two shift axes; -0.9 -0.4 nm on specimen axes Rate = 5.05031 A/sec Drift is low enough after shot 3 Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.07 nm/sec Measured defocus = -4.75 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -4.475 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_053_44.0_Oct23_07.35.14.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 87.0 In SetExposureForMean 90, exposure time changed to 2.5600 Image shift 0.035 -0.128 IS units 380.7 24.8 unbinned pixels; need stage -0.020 0.186 if reset Tilting to -64 -64.00 degrees 4.5 -6.2 unbinned pixels; 2.6 0.8 nm along two shift axes; 2.0 1.8 nm on specimen axes Rate = 13.50454 A/sec 3.8 2.5 unbinned pixels; 0.4 1.5 nm along two shift axes; -1.0 1.2 nm on specimen axes Rate = 7.94765 A/sec Drift is low enough after shot 2 Measured defocus = -4.80 microns changed by 0.05 to target drift = 0.03 nm/sec Measured defocus = -4.77 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = 0.103 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_054_-64.0_Oct23_07.36.26.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 64.3 In SetExposureForMean 90, exposure time changed to 3.6800 Image shift 0.363 -0.909 IS units 2820.2 -123.7 unbinned pixels; need stage -0.044 2.284 if reset Omitting tilt at -66 Tilting to 46 Correcting tilt backlash -19.0 -0.1 unbinned pixels; 2.2 6.3 nm along two shift axes; 0.6 -6.7 nm on specimen axes Rate = 33.32219 A/sec -9.4 4.3 unbinned pixels; 2.5 2.6 nm along two shift axes; -1.2 -3.4 nm on specimen axes Rate = 18.04732 A/sec -11.6 4.2 unbinned pixels; 2.8 3.3 nm along two shift axes; -1.1 -4.2 nm on specimen axes Rate = 21.57135 A/sec -2.9 1.7 unbinned pixels; 0.9 0.7 nm along two shift axes; -0.5 -1.1 nm on specimen axes Rate = 5.79345 A/sec Drift is low enough after shot 4 Measured defocus = -4.60 microns changed by -0.15 to target drift = 0.10 nm/sec Measured defocus = -4.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -4.611 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_055_46.0_Oct23_07.37.55.tif Saved Z = 55, 46.00 degrees Mean of buffer A = 126.2 In SetExposureForMean 90, exposure time changed to 2.7200 Image shift 0.034 -0.151 IS units 441.7 52.1 unbinned pixels; need stage -0.032 0.222 if reset Tilting to 48 Correcting tilt backlash -6.3 -7.6 unbinned pixels; 1.8 3.0 nm along two shift axes; 2.9 -2.0 nm on specimen axes Rate = 17.50996 A/sec -2.5 6.6 unbinned pixels; 2.5 0.1 nm along two shift axes; -2.2 -1.1 nm on specimen axes Rate = 12.44583 A/sec -0.9 3.4 unbinned pixels; 1.2 0.1 nm along two shift axes; -1.2 -0.4 nm on specimen axes Rate = 6.18543 A/sec Drift is low enough after shot 3 Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.08 nm/sec Measured defocus = -4.74 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -4.629 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\201022_Jans_cells\frames\batch_tomo_10_20_056_48.0_Oct23_07.39.07.tif Saved Z = 56, 48.00 degrees Mean of buffer A = 87.7 In SetExposureForMean 90, exposure time changed to 2.8800 Image shift 0.102 -0.276 IS units 848.5 -14.6 unbinned pixels; need stage -0.021 0.449 if reset Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -3.04 microns changed by -1.71 to target drift = 0.25 nm/sec