Angle = -4.40, increment = 0.60, shift = -151.5 -25.5 pixels, -0.350 um Y Trimming A by 20 35, reference by 20 50 Angle = -3.20, increment = 1.80, shift = -444.4 -68.9 pixels, -1.026 um Y Trimming A by 20 30, reference by 20 50 Angle = -1.48, increment = 3.52, shift = -867.5 -131.2 pixels, -2.002 um Y Rough eucentricity: changing Z by 32.68 to -178.18, continuing... Trimming A by 20 21 35 20, reference by 20 20 30 20 Angle = 2.03, increment = 3.52, shift = 84.4 -40.1 pixels, 0.181 um Y Trimming A by 20 30, reference by 20 25 Angle = 6.52, increment = 8.00, shift = 195.9 -37.3 pixels, 0.435 um Y Rough eucentricity: changing Z by -3.12 to -181.30, finished. Angle = -4.40, increment = 0.60, shift = 8.4 -0.4 pixels, 0.019 um Y Angle = -3.20, increment = 1.80, shift = 15.3 1.3 pixels, 0.035 um Y Angle = 0.41, increment = 5.41, shift = 30.7 1.6 pixels, 0.070 um Y Trimming A by 25 25, reference by 20 20 Angle = 3.00, increment = 8.00, shift = 53.6 1.9 pixels, 0.122 um Y Rough eucentricity: changing Z by -0.88 to -182.17, finished. Measured defocus = 2.46 microns changed by -5.96 to target drift = 0.12 nm/sec Measured defocus = -4.12 microns changed by 0.62 to target drift = 0.13 nm/sec Trimming A by 24 24 220 20, reference by 22 23 205 20 Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.029 um; position 0.084, 0.337 Drift reached 2.4 A/sec after 5.5 sec, going on Measured defocus = -3.67 microns changed by -0.33 to target drift = 0.14 nm/sec Tracking image shifted -0.000, 0.006 um; position 0.084, 0.329 Running script # 14 2.0 1.2 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.5 0.7 nm on specimen axes Rate = 4.06952 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_10.54.03_tomo_01_000_-10.0.tif Record image shifted -0.064, -0.002 um Tracking reference shifted by 9.6,182.8 pixels, -0.064,-0.002 um Saved Z = 0 at 0.00 min Tilt = -10.00 Mean = 93 Exp = 1.560 X/Y/Z = 0.149 0.331 2.188 Need regular tracking - Need autofocus Tracking image shifted -0.005, -0.023 um; position 0.154, 0.354 Drift reached 0.4 A/sec after 5.7 sec, going on Measured defocus = -4.12 microns changed by 0.12 to target drift = 0.05 nm/sec Tracking image shifted -0.002, -0.001 um; position 0.156, 0.356 Running script # 14 0.1 1.1 unbinned pixels; 0.4 0.2 nm along two shift axes; -0.4 -0.0 nm on specimen axes Rate = 1.98524 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_10.54.45_tomo_01_001_-12.0.tif Record image shifted -0.001, -0.006 um Tracking reference shifted by -16.1,4.5 pixels, -0.001,-0.006 um Saved Z = 1 at 0.70 min Tilt = -12.00 Mean = 101 Exp = 1.674 X/Y/Z = 0.158 0.362 2.304 Predicted X = 0.167 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.392 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 2.421 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.086 um; position 0.161, 0.306 Drift reached 5.5 A/sec after 5.7 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.29 nm/sec Tracking image shifted 0.001, 0.012 um; position 0.160, 0.291 Running script # 14 4.5 0.6 unbinned pixels; 0.3 1.6 nm along two shift axes; -0.4 1.6 nm on specimen axes Rate = 7.98059 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_10.55.27_tomo_01_002_-14.0.tif Record image shifted -0.001, -0.001 um Tracking reference shifted by -3.4,2.1 pixels, -0.001,-0.001 um Saved Z = 2 at 1.39 min Tilt = -14.00 Mean = 99 Exp = 1.656 X/Y/Z = 0.161 0.291 2.399 Errors in X/Y Prediction -0.006 -0.101 um, 0.4 7.8 % Error in Z Prediction -0.022 Need regular tracking - Need autofocus Tracking image shifted -0.030, 0.086 um; position 0.179, 0.245 Drift reached 0.4 A/sec after 5.7 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.05 nm/sec Tracking image shifted -0.000, -0.002 um; position 0.179, 0.248 Running script # 14 -0.3 0.9 unbinned pixels; 0.3 0.0 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.63841 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_10.56.11_tomo_01_003_-8.0.tif Record image shifted -0.002, -0.000 um Tracking reference shifted by -0.8,6.3 pixels, -0.002,-0.000 um Saved Z = 3 at 2.13 min Tilt = -8.00 Mean = 102 Exp = 1.674 X/Y/Z = 0.181 0.248 2.170 Need regular tracking - Need autofocus Tracking image shifted -0.010, -0.023 um; position 0.191, 0.271 Drift reached 3.1 A/sec after 5.7 sec, going on Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.13 nm/sec Tracking image shifted -0.001, 0.008 um; position 0.192, 0.261 Running script # 14 1.5 0.4 unbinned pixels; 0.0 0.5 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.74298 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_10.56.55_tomo_01_004_-6.0.tif Record image shifted -0.000, 0.005 um Tracking reference shifted by 13.9,-0.9 pixels, -0.000,0.005 um Saved Z = 4 at 2.87 min Tilt = -6.00 Mean = 100 Exp = 1.649 X/Y/Z = 0.192 0.256 2.086 Predicted X = 0.168 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.288 StdErr = 0.076 nFit = 3 nDrop = 0 Predicted Z = 2.508 StdErr = 0.016 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.030, 0.041 um; position 0.198, 0.247 Drift reached 0.3 A/sec after 5.6 sec, going on Running script # 14 -0.5 0.8 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.2 nm on specimen axes Rate = 1.70401 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_10.57.26_tomo_01_005_-16.0.tif Record image shifted -0.002, -0.010 um Tracking reference shifted by -26.6,7.8 pixels, -0.002,-0.010 um Saved Z = 5 at 3.45 min Tilt = -16.00 Mean = 99 Exp = 1.669 X/Y/Z = 0.201 0.256 2.508 Errors in X/Y Prediction 0.033 -0.031 um, 2.5 2.4 % Predicted X = 0.216 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Y = 0.198 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Z = 2.614 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, -0.072 um; position 0.203, 0.270 Drift reached 4.9 A/sec after 5.7 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.24 nm/sec Tracking image shifted 0.000, 0.010 um; position 0.203, 0.257 Running script # 14 2.4 -0.0 unbinned pixels; 0.3 0.8 nm along two shift axes; -0.1 0.8 nm on specimen axes Rate = 4.22316 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_10.58.17_tomo_01_006_-18.0.tif Record image shifted -0.000, 0.002 um Tracking reference shifted by 4.5,0.8 pixels, -0.000,0.002 um Saved Z = 6 at 4.24 min Tilt = -18.00 Mean = 99 Exp = 1.686 X/Y/Z = 0.204 0.254 2.559 Errors in X/Y Prediction -0.012 0.056 um, 0.9 4.3 % Error in Z Prediction -0.055 Predicted X = 0.203 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.264 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 2.003 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.015, 0.045 um; position 0.218, 0.219 Drift reached 0.4 A/sec after 5.7 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.04 nm/sec Tracking image shifted -0.000, -0.005 um; position 0.218, 0.224 Running script # 14 -0.6 0.8 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.2 nm on specimen axes Rate = 1.66725 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_10.59.08_tomo_01_007_-4.0.tif Record image shifted -0.002, 0.000 um Tracking reference shifted by 1.5,5.8 pixels, -0.002,0.000 um Saved Z = 7 at 5.08 min Tilt = -4.00 Mean = 100 Exp = 1.645 X/Y/Z = 0.221 0.223 2.006 Errors in X/Y Prediction 0.018 -0.040 um, 1.4 3.1 % Error in Z Prediction 0.003 Predicted X = 0.237 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = 0.218 StdErr = 0.030 nFit = 3 nDrop = 0 Predicted Z = 1.923 StdErr = 0.002 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.016, -0.005 um; position 0.222, 0.223 Drift reached 3.4 A/sec after 5.7 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.13 nm/sec Tracking image shifted 0.000, 0.008 um; position 0.222, 0.213 Running script # 14 1.5 0.4 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.80557 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_10.59.52_tomo_01_008_-2.0.tif Record image shifted -0.000, 0.006 um Tracking reference shifted by 15.8,-1.4 pixels, -0.000,0.006 um Saved Z = 8 at 5.81 min Tilt = -2.00 Mean = 100 Exp = 1.649 X/Y/Z = 0.222 0.207 1.910 Errors in X/Y Prediction -0.015 -0.011 um, 1.2 0.8 % Error in Z Prediction -0.013 Predicted X = 0.231 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Y = 0.230 StdErr = 0.024 nFit = 3 nDrop = 0 Predicted Z = 2.645 StdErr = 0.014 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, -0.033 um; position 0.231, 0.264 Drift reached 0.7 A/sec after 5.7 sec, going on Running script # 14 -0.1 0.1 unbinned pixels; 0.1 0.0 nm along two shift axes; -0.0 -0.0 nm on specimen axes Rate = 0.28285 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.00.23_tomo_01_009_-20.0.tif Record image shifted -0.002, -0.008 um Tracking reference shifted by -22.8,6.4 pixels, -0.002,-0.008 um Saved Z = 9 at 6.33 min Tilt = -20.00 Mean = 99 Exp = 1.698 X/Y/Z = 0.233 0.271 2.645 Errors in X/Y Prediction 0.002 0.041 um, 0.1 3.1 % Predicted X = 0.245 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.318 StdErr = 0.009 nFit = 4 nDrop = 0 Predicted Z = 2.729 StdErr = 0.016 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, 0.011 um; position 0.236, 0.307 Drift reached 3.8 A/sec after 5.7 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.19 nm/sec Tracking image shifted 0.001, 0.008 um; position 0.235, 0.296 Running script # 14 2.0 -0.0 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.1 0.7 nm on specimen axes Rate = 3.58561 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.01.04_tomo_01_010_-22.0.tif Record image shifted -0.000, -0.001 um Tracking reference shifted by -3.0,1.5 pixels, -0.000,-0.001 um Saved Z = 10 at 7.02 min Tilt = -22.00 Mean = 99 Exp = 1.718 X/Y/Z = 0.236 0.296 2.698 Errors in X/Y Prediction -0.009 -0.021 um, 0.7 1.6 % Error in Z Prediction -0.030 Predicted X = 0.241 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.180 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Z = 1.825 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, -0.007 um; position 0.238, 0.187 Drift reached 1.6 A/sec after 5.6 sec, going on Running script # 14 -2.8 0.6 unbinned pixels; 0.5 0.9 nm along two shift axes; -0.1 -1.0 nm on specimen axes Rate = 5.10012 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.01.38_tomo_01_011_-0.0.tif Record image shifted -0.002, -0.003 um Tracking reference shifted by -7.2,5.8 pixels, -0.002,-0.003 um Saved Z = 11 at 7.58 min Tilt = -0.00 Mean = 100 Exp = 1.649 X/Y/Z = 0.240 0.192 1.825 Errors in X/Y Prediction -0.001 0.012 um, 0.1 0.9 % Predicted X = 0.247 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = 0.176 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Z = 1.737 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, 0.005 um; position 0.240, 0.171 Drift reached 3.5 A/sec after 5.6 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.15 nm/sec Tracking image shifted -0.000, 0.007 um; position 0.240, 0.161 Running script # 14 1.5 0.5 unbinned pixels; 0.0 0.5 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.68356 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.02.22_tomo_01_012_2.0.tif Record image shifted 0.000, 0.009 um Tracking reference shifted by 24.7,-2.2 pixels, 0.000,0.009 um Saved Z = 12 at 8.31 min Tilt = 2.00 Mean = 100 Exp = 1.655 X/Y/Z = 0.240 0.152 1.740 Errors in X/Y Prediction -0.007 -0.024 um, 0.5 1.8 % Error in Z Prediction 0.003 Predicted X = 0.256 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.351 StdErr = 0.013 nFit = 5 nDrop = 0 Predicted Z = 2.777 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, 0.048 um; position 0.253, 0.302 Drift reached 1.2 A/sec after 5.7 sec, going on Running script # 14 1.0 0.5 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.94534 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.02.54_tomo_01_013_-24.0.tif Record image shifted 0.000, -0.006 um Tracking reference shifted by -16.0,1.3 pixels, 0.000,-0.006 um Saved Z = 13 at 8.85 min Tilt = -24.00 Mean = 99 Exp = 1.738 X/Y/Z = 0.253 0.307 2.777 Errors in X/Y Prediction -0.003 -0.043 um, 0.2 3.3 % Predicted X = 0.261 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = 0.337 StdErr = 0.018 nFit = 5 nDrop = 0 Predicted Z = 2.852 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, 0.005 um; position 0.254, 0.332 Drift reached 2.8 A/sec after 5.7 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.15 nm/sec Tracking image shifted 0.001, 0.007 um; position 0.253, 0.324 Running script # 14 1.7 0.1 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.1 0.6 nm on specimen axes Rate = 2.91479 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.03.35_tomo_01_014_-26.0.tif Record image shifted -0.002, -0.003 um Tracking reference shifted by -8.9,6.5 pixels, -0.002,-0.003 um Saved Z = 14 at 9.53 min Tilt = -26.00 Mean = 98 Exp = 1.764 X/Y/Z = 0.255 0.327 2.800 Errors in X/Y Prediction -0.005 -0.011 um, 0.4 0.8 % Error in Z Prediction -0.052 Predicted X = 0.249 StdErr = 0.023 nFit = 4 nDrop = 0 Predicted Y = 0.107 StdErr = 0.017 nFit = 4 nDrop = 0 Predicted Z = 1.650 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.033 um; position 0.247, 0.140 Drift reached 1.3 A/sec after 5.7 sec, going on Running script # 14 -3.0 1.1 unbinned pixels; 0.7 0.9 nm along two shift axes; -0.3 -1.1 nm on specimen axes Rate = 5.58632 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.04.09_tomo_01_015_4.0.tif Record image shifted -0.002, -0.003 um Tracking reference shifted by -9.2,7.0 pixels, -0.002,-0.003 um Saved Z = 15 at 10.10 min Tilt = 4.00 Mean = 99 Exp = 1.661 X/Y/Z = 0.250 0.145 1.650 Errors in X/Y Prediction 0.002 0.038 um, 0.1 2.9 % Predicted X = 0.249 StdErr = 0.018 nFit = 4 nDrop = 0 Predicted Y = 0.118 StdErr = 0.024 nFit = 5 nDrop = 0 Predicted Z = 1.562 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, -0.015 um; position 0.247, 0.132 Drift reached 3.4 A/sec after 5.7 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.13 nm/sec Tracking image shifted -0.001, 0.006 um; position 0.248, 0.124 Running script # 14 1.4 0.2 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.1 0.5 nm on specimen axes Rate = 2.46238 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.04.53_tomo_01_016_6.0.tif Record image shifted 0.000, 0.011 um Tracking reference shifted by 29.7,-4.0 pixels, 0.000,0.011 um Saved Z = 16 at 10.84 min Tilt = 6.00 Mean = 99 Exp = 1.672 X/Y/Z = 0.247 0.113 1.590 Errors in X/Y Prediction -0.002 -0.004 um, 0.1 0.3 % Error in Z Prediction 0.028 Predicted X = 0.268 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = 0.340 StdErr = 0.009 nFit = 5 nDrop = 1 Predicted Z = 2.866 StdErr = 0.025 nFit = 3 nDrop = 0 Drift reached 1.2 A/sec after 5.4 sec, going on Running script # 14 3.6 -0.4 unbinned pixels; 0.6 1.2 nm along two shift axes; 0.0 1.3 nm on specimen axes Rate = 6.40182 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.05.25_tomo_01_017_-28.0.tif Record image shifted 0.002, 0.043 um Saved Z = 17 at 11.36 min Tilt = -28.00 Mean = 99 Exp = 1.794 X/Y/Z = 0.266 0.296 2.866 Errors in X/Y Prediction -0.002 -0.045 um, 0.1 3.4 % Predicted X = 0.272 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = 0.272 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 2.927 StdErr = 0.029 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.009, -0.015 um; position 0.263, 0.286 Drift reached 0.3 A/sec after 5.9 sec, going on Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.02 nm/sec Tracking image shifted -0.001, 0.001 um; position 0.263, 0.285 Running script # 14 0.4 0.4 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 0.98207 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.06.08_tomo_01_018_-30.0.tif Record image shifted -0.002, -0.011 um Tracking reference shifted by -30.3,8.1 pixels, -0.002,-0.011 um Saved Z = 18 at 12.08 min Tilt = -30.00 Mean = 98 Exp = 1.820 X/Y/Z = 0.266 0.295 2.965 Errors in X/Y Prediction -0.006 0.024 um, 0.5 1.8 % Error in Z Prediction 0.038 Predicted X = 0.249 StdErr = 0.015 nFit = 4 nDrop = 0 Predicted Y = 0.087 StdErr = 0.017 nFit = 6 nDrop = 0 Predicted Z = 1.507 StdErr = 0.014 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.011, 0.033 um; position 0.260, 0.054 Drift reached 1.7 A/sec after 5.7 sec, going on Running script # 14 -3.9 1.1 unbinned pixels; 0.8 1.1 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 7.01926 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.06.43_tomo_01_019_8.0.tif Record image shifted -0.002, -0.004 um Tracking reference shifted by -10.1,6.0 pixels, -0.002,-0.004 um Saved Z = 19 at 12.66 min Tilt = 8.00 Mean = 99 Exp = 1.683 X/Y/Z = 0.262 0.060 1.507 Errors in X/Y Prediction 0.013 -0.027 um, 1.0 2.1 % Predicted X = 0.271 StdErr = 0.020 nFit = 4 nDrop = 0 Predicted Y = 0.021 StdErr = 0.023 nFit = 6 nDrop = 0 Predicted Z = 1.427 StdErr = 0.016 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, -0.003 um; position 0.258, 0.024 Drift reached 3.2 A/sec after 5.6 sec, going on Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.13 nm/sec Tracking image shifted -0.001, 0.007 um; position 0.259, 0.015 Running script # 14 1.3 0.5 unbinned pixels; 0.0 0.5 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.44555 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.07.27_tomo_01_020_10.0.tif Record image shifted -0.000, 0.012 um Tracking reference shifted by 33.4,-2.1 pixels, -0.000,0.012 um Saved Z = 20 at 13.40 min Tilt = 10.00 Mean = 99 Exp = 1.704 X/Y/Z = 0.259 0.003 1.427 Errors in X/Y Prediction -0.012 -0.018 um, 0.9 1.4 % Error in Z Prediction -0.000 Predicted X = 0.268 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Y = 0.264 StdErr = 0.030 nFit = 5 nDrop = 0 Predicted Z = 3.021 StdErr = 0.041 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.009, 0.013 um; position 0.277, 0.251 Drift reached 2.6 A/sec after 5.9 sec, going on Running script # 14 3.7 -0.2 unbinned pixels; 0.5 1.2 nm along two shift axes; -0.1 1.3 nm on specimen axes Rate = 6.577 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.08.01_tomo_01_021_-32.0.tif Record image shifted -0.001, -0.000 um Tracking reference shifted by -0.1,2.9 pixels, -0.001,-0.000 um Saved Z = 21 at 13.97 min Tilt = -32.00 Mean = 97 Exp = 1.860 X/Y/Z = 0.278 0.248 3.021 Errors in X/Y Prediction 0.010 -0.016 um, 0.8 1.2 % Predicted X = 0.282 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.199 StdErr = 0.032 nFit = 5 nDrop = 0 Predicted Z = 3.088 StdErr = 0.048 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.068 um; position 0.278, 0.267 Drift reached 1.1 A/sec after 5.9 sec, going on Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.03 nm/sec Tracking image shifted -0.001, -0.002 um; position 0.280, 0.269 Running script # 14 0.1 0.4 unbinned pixels; 0.1 0.1 nm along two shift axes; -0.2 0.0 nm on specimen axes Rate = 0.81099 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.08.44_tomo_01_022_-34.0.tif Record image shifted -0.001, -0.010 um Tracking reference shifted by -27.6,5.5 pixels, -0.001,-0.010 um Saved Z = 22 at 14.68 min Tilt = -34.00 Mean = 98 Exp = 1.911 X/Y/Z = 0.281 0.279 3.166 Errors in X/Y Prediction -0.001 0.080 um, 0.1 6.1 % Error in Z Prediction 0.078 Predicted X = 0.268 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.081 StdErr = 0.011 nFit = 5 nDrop = 1 Predicted Z = 1.347 StdErr = 0.009 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.017 um; position 0.267, -0.065 Drift reached 2.8 A/sec after 5.7 sec, going on Running script # 14 -5.4 1.2 unbinned pixels; 1.0 1.6 nm along two shift axes; -0.3 -1.9 nm on specimen axes Rate = 9.70082 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.09.19_tomo_01_023_12.0.tif Record image shifted -0.002, -0.003 um Tracking reference shifted by -6.8,7.4 pixels, -0.002,-0.003 um Saved Z = 23 at 15.26 min Tilt = 12.00 Mean = 99 Exp = 1.718 X/Y/Z = 0.270 -0.058 1.347 Errors in X/Y Prediction 0.002 0.023 um, 0.1 1.8 % Predicted X = 0.272 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.134 StdErr = 0.010 nFit = 5 nDrop = 1 Predicted Z = 1.267 StdErr = 0.010 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.021 um; position 0.266, -0.156 Drift reached 3.4 A/sec after 5.7 sec, going on Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.15 nm/sec Tracking image shifted -0.001, 0.009 um; position 0.267, -0.167 Running script # 14 4.8 1.7 unbinned pixels; 0.0 1.8 nm along two shift axes; -0.7 1.6 nm on specimen axes Rate = 8.91463 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.10.03_tomo_01_024_14.0.tif Record image shifted 0.000, 0.009 um Tracking reference shifted by 26.7,-3.7 pixels, 0.000,0.009 um Saved Z = 24 at 15.99 min Tilt = 14.00 Mean = 99 Exp = 1.741 X/Y/Z = 0.268 -0.178 1.305 Errors in X/Y Prediction -0.004 -0.044 um, 0.3 3.3 % Error in Z Prediction 0.039 Predicted X = 0.290 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = 0.284 StdErr = 0.047 nFit = 5 nDrop = 0 Predicted Z = 3.251 StdErr = 0.022 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, 0.047 um; position 0.289, 0.237 Drift reached 3.4 A/sec after 6.1 sec, going on Running script # 14 6.5 0.6 unbinned pixels; 0.6 2.2 nm along two shift axes; -0.4 2.3 nm on specimen axes Rate = 11.5494 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.10.42_tomo_01_025_-36.0.tif Record image shifted 0.000, -0.002 um Tracking reference shifted by -6.4,0.3 pixels, 0.000,-0.002 um Saved Z = 25 at 16.66 min Tilt = -36.00 Mean = 97 Exp = 1.955 X/Y/Z = 0.289 0.235 3.251 Errors in X/Y Prediction -0.001 -0.049 um, 0.1 3.7 % Predicted X = 0.294 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = 0.225 StdErr = 0.057 nFit = 5 nDrop = 0 Predicted Z = 3.343 StdErr = 0.026 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.027 um; position 0.289, 0.252 Drift reached 1.3 A/sec after 6.1 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.002 um; position 0.290, 0.249 Running script # 14 0.5 0.6 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.33561 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.11.26_tomo_01_026_-38.0.tif Record image shifted -0.002, -0.009 um Tracking reference shifted by -25.4,8.5 pixels, -0.002,-0.009 um Saved Z = 26 at 17.38 min Tilt = -38.00 Mean = 97 Exp = 2.011 X/Y/Z = 0.293 0.258 3.329 Errors in X/Y Prediction -0.001 0.034 um, 0.1 2.6 % Error in Z Prediction -0.013 Predicted X = 0.274 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.290 StdErr = 0.031 nFit = 5 nDrop = 0 Predicted Z = 1.222 StdErr = 0.019 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.006, -0.058 um; position 0.268, -0.233 Drift reached 3.9 A/sec after 5.7 sec, going on Running script # 14 -8.3 2.7 unbinned pixels; 1.8 2.4 nm along two shift axes; -0.7 -3.0 nm on specimen axes Rate = 15.2265 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.12.02_tomo_01_027_16.0.tif Record image shifted -0.004, -0.007 um Tracking reference shifted by -18.5,14.1 pixels, -0.004,-0.007 um Saved Z = 27 at 17.99 min Tilt = 16.00 Mean = 98 Exp = 1.765 X/Y/Z = 0.274 -0.220 1.222 Errors in X/Y Prediction -0.000 0.070 um, 0.0 5.4 % Predicted X = 0.274 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.316 StdErr = 0.057 nFit = 5 nDrop = 0 Predicted Z = 1.149 StdErr = 0.021 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, -0.011 um; position 0.275, -0.304 Drift reached 3.4 A/sec after 5.9 sec, going on Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.13 nm/sec Tracking image shifted -0.002, 0.008 um; position 0.277, -0.314 Running script # 14 1.5 0.3 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.67827 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.12.48_tomo_01_028_18.0.tif Record image shifted 0.001, 0.018 um Tracking reference shifted by 49.7,-8.6 pixels, 0.001,0.018 um Saved Z = 28 at 18.75 min Tilt = 18.00 Mean = 99 Exp = 1.800 X/Y/Z = 0.275 -0.332 1.215 Errors in X/Y Prediction 0.001 -0.017 um, 0.1 1.3 % Error in Z Prediction 0.067 Predicted X = 0.300 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.270 StdErr = 0.059 nFit = 5 nDrop = 0 Predicted Z = 3.426 StdErr = 0.024 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, 0.090 um; position 0.298, 0.180 Drift reached 3.4 A/sec after 6.2 sec, going on Running script # 14 4.0 0.4 unbinned pixels; 0.3 1.4 nm along two shift axes; -0.3 1.4 nm on specimen axes Rate = 6.98861 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.13.23_tomo_01_029_-40.0.tif Record image shifted 0.000, -0.003 um Tracking reference shifted by -9.5,-0.3 pixels, 0.000,-0.003 um Saved Z = 29 at 19.34 min Tilt = -40.00 Mean = 97 Exp = 2.075 X/Y/Z = 0.298 0.180 3.426 Errors in X/Y Prediction -0.001 -0.090 um, 0.1 6.9 % Predicted X = 0.302 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.118 StdErr = 0.064 nFit = 5 nDrop = 0 Predicted Z = 3.517 StdErr = 0.027 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.067 um; position 0.296, 0.185 Drift reached 1.8 A/sec after 6.3 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.07 nm/sec Tracking image shifted -0.001, 0.004 um; position 0.297, 0.180 Running script # 14 0.8 0.2 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.1 0.3 nm on specimen axes Rate = 1.50664 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.14.08_tomo_01_030_-42.0.tif Record image shifted -0.003, -0.011 um Tracking reference shifted by -31.1,12.0 pixels, -0.003,-0.011 um Saved Z = 30 at 20.09 min Tilt = -42.00 Mean = 96 Exp = 2.141 X/Y/Z = 0.301 0.191 3.498 Errors in X/Y Prediction -0.002 0.073 um, 0.1 5.6 % Error in Z Prediction -0.020 Predicted X = 0.305 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.163 StdErr = 0.073 nFit = 5 nDrop = 0 Predicted Z = 3.580 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.005, 0.011 um; position 0.301, 0.152 Drift reached 1.1 A/sec after 6.3 sec, going on Running script # 14 2.0 0.2 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.1 0.7 nm on specimen axes Rate = 3.53615 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.14.39_tomo_01_031_-44.0.tif Record image shifted -0.000, -0.006 um Tracking reference shifted by -15.5,2.3 pixels, -0.000,-0.006 um Saved Z = 31 at 20.61 min Tilt = -44.00 Mean = 96 Exp = 2.228 X/Y/Z = 0.301 0.156 3.580 Errors in X/Y Prediction -0.004 -0.007 um, 0.3 0.5 % Predicted X = 0.280 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.419 StdErr = 0.056 nFit = 5 nDrop = 0 Predicted Z = 1.157 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.010, 0.001 um; position 0.269, -0.420 Drift reached 5.7 A/sec after 5.9 sec, going on Running script # 14 -9.6 3.6 unbinned pixels; 2.3 2.7 nm along two shift axes; -1.0 -3.5 nm on specimen axes Rate = 17.9198 A/sec -8.0 2.6 unbinned pixels; 1.8 2.3 nm along two shift axes; -0.7 -2.9 nm on specimen axes Rate = 14.83063 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.15.22_tomo_01_032_20.0.tif Record image shifted -0.005, -0.009 um Tracking reference shifted by -24.7,15.3 pixels, -0.005,-0.009 um Saved Z = 32 at 21.31 min Tilt = 20.00 Mean = 97 Exp = 1.821 X/Y/Z = 0.276 -0.401 1.157 Errors in X/Y Prediction -0.004 0.019 um, 0.3 1.4 % Predicted X = 0.278 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -0.473 StdErr = 0.055 nFit = 5 nDrop = 0 Predicted Z = 1.104 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.011 um; position 0.279, -0.462 Drift reached 3.3 A/sec after 5.9 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.14 nm/sec Tracking image shifted -0.002, 0.009 um; position 0.281, -0.473 Running script # 14 1.5 1.0 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.4 0.5 nm on specimen axes Rate = 3.1096 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.16.06_tomo_01_033_22.0.tif Record image shifted 0.001, 0.020 um Tracking reference shifted by 56.6,-8.5 pixels, 0.001,0.020 um Saved Z = 33 at 22.06 min Tilt = 22.00 Mean = 98 Exp = 1.870 X/Y/Z = 0.280 -0.494 1.057 Errors in X/Y Prediction 0.003 -0.021 um, 0.2 1.6 % Error in Z Prediction -0.047 Predicted X = 0.282 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.598 StdErr = 0.043 nFit = 5 nDrop = 0 Predicted Z = 1.006 StdErr = 0.044 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.017, -0.050 um; position 0.265, -0.548 Drift reached 3.8 A/sec after 5.9 sec, going on Running script # 14 -7.0 2.4 unbinned pixels; 1.6 2.0 nm along two shift axes; -0.6 -2.5 nm on specimen axes Rate = 12.99478 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.16.38_tomo_01_034_24.0.tif Record image shifted -0.002, -0.004 um Tracking reference shifted by -10.6,7.2 pixels, -0.002,-0.004 um Saved Z = 34 at 22.59 min Tilt = 24.00 Mean = 97 Exp = 1.912 X/Y/Z = 0.269 -0.539 1.006 Errors in X/Y Prediction -0.014 0.059 um, 1.1 4.5 % Predicted X = 0.303 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.124 StdErr = 0.072 nFit = 5 nDrop = 0 Predicted Z = 3.663 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, 0.087 um; position 0.300, 0.036 Drift reached 3.8 A/sec after 6.5 sec, going on Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.15 nm/sec Tracking image shifted 0.002, 0.006 um; position 0.299, 0.027 Running script # 14 2.0 -0.2 unbinned pixels; 0.3 0.6 nm along two shift axes; 0.0 0.7 nm on specimen axes Rate = 3.57293 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.17.29_tomo_01_035_-46.0.tif Record image shifted -0.003, -0.008 um Tracking reference shifted by -23.2,10.4 pixels, -0.003,-0.008 um Saved Z = 35 at 23.45 min Tilt = -46.00 Mean = 95 Exp = 2.312 X/Y/Z = 0.301 0.035 3.701 Errors in X/Y Prediction -0.001 -0.088 um, 0.1 6.8 % Error in Z Prediction 0.037 Predicted X = 0.302 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -0.043 StdErr = 0.095 nFit = 5 nDrop = 0 Predicted Z = 3.788 StdErr = 0.022 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, -0.043 um; position 0.300, 0.001 Drift reached 1.6 A/sec after 6.7 sec, going on Running script # 14 1.8 0.3 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.2 0.6 nm on specimen axes Rate = 3.19713 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.18.02_tomo_01_036_-48.0.tif Record image shifted -0.002, -0.009 um Tracking reference shifted by -25.7,8.3 pixels, -0.002,-0.009 um Saved Z = 36 at 24.00 min Tilt = -48.00 Mean = 95 Exp = 2.431 X/Y/Z = 0.302 0.008 3.788 Errors in X/Y Prediction 0.001 0.051 um, 0.0 3.9 % Predicted X = 0.303 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -0.117 StdErr = 0.086 nFit = 5 nDrop = 0 Predicted Z = 3.881 StdErr = 0.025 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.084 um; position 0.302, -0.033 Drift reached 3.2 A/sec after 6.7 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.15 nm/sec Tracking image shifted 0.001, 0.004 um; position 0.302, -0.040 Running script # 14 2.1 -0.5 unbinned pixels; 0.4 0.6 nm along two shift axes; 0.1 0.7 nm on specimen axes Rate = 3.72019 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.18.49_tomo_01_037_-50.0.tif Record image shifted -0.001, -0.014 um Tracking reference shifted by -39.5,5.7 pixels, -0.001,-0.014 um Saved Z = 37 at 24.78 min Tilt = -50.00 Mean = 95 Exp = 2.552 X/Y/Z = 0.303 -0.026 3.859 Errors in X/Y Prediction -0.000 0.091 um, 0.0 6.9 % Error in Z Prediction -0.022 Predicted X = 0.303 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -0.055 StdErr = 0.070 nFit = 5 nDrop = 0 Predicted Z = 3.956 StdErr = 0.029 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, 0.062 um; position 0.299, -0.117 Drift reached 1.5 A/sec after 6.9 sec, going on Running script # 14 2.4 -0.2 unbinned pixels; 0.3 0.8 nm along two shift axes; -0.0 0.9 nm on specimen axes Rate = 4.2892 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.19.23_tomo_01_038_-52.0.tif Record image shifted -0.000, -0.011 um Tracking reference shifted by -30.9,3.8 pixels, -0.000,-0.011 um Saved Z = 38 at 25.42 min Tilt = -52.00 Mean = 94 Exp = 2.699 X/Y/Z = 0.300 -0.108 3.956 Errors in X/Y Prediction -0.003 -0.053 um, 0.3 4.0 % Predicted X = 0.268 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.583 StdErr = 0.029 nFit = 5 nDrop = 0 Predicted Z = 0.944 StdErr = 0.051 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.008, -0.020 um; position 0.276, -0.563 Drift reached 4.6 A/sec after 6.1 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.32 nm/sec Tracking image shifted -0.003, -0.014 um; position 0.280, -0.545 Running script # 14 -2.6 3.3 unbinned pixels; 1.4 0.5 nm along two shift axes; -1.1 -1.0 nm on specimen axes Rate = 7.28492 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.20.20_tomo_01_039_26.0.tif Record image shifted -0.005, 0.010 um Tracking reference shifted by 30.1,10.3 pixels, -0.005,0.010 um Saved Z = 39 at 26.36 min Tilt = 26.00 Mean = 98 Exp = 1.966 X/Y/Z = 0.285 -0.555 0.931 Errors in X/Y Prediction 0.018 0.028 um, 1.4 2.1 % Error in Z Prediction -0.013 Predicted X = 0.283 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Y = -0.562 StdErr = 0.034 nFit = 5 nDrop = 0 Predicted Z = 0.854 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.105 um; position 0.284, -0.668 Drift reached 2.0 A/sec after 6.0 sec, going on Running script # 14 1.9 0.0 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.1 0.7 nm on specimen axes Rate = 3.40075 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.20.57_tomo_01_040_28.0.tif Record image shifted 0.002, 0.016 um Tracking reference shifted by 43.9,-9.0 pixels, 0.002,0.016 um Saved Z = 40 at 26.91 min Tilt = 28.00 Mean = 97 Exp = 2.016 X/Y/Z = 0.283 -0.685 0.854 Errors in X/Y Prediction -0.000 -0.123 um, 0.0 9.4 % Predicted X = 0.293 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = -0.746 StdErr = 0.090 nFit = 5 nDrop = 0 Predicted Z = 0.783 StdErr = 0.024 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.026, 0.090 um; position 0.267, -0.836 Drift reached 1.7 A/sec after 6.1 sec, going on Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.10 nm/sec Tracking image shifted 0.001, -0.006 um; position 0.267, -0.830 Running script # 14 -1.8 0.6 unbinned pixels; 0.4 0.5 nm along two shift axes; -0.2 -0.6 nm on specimen axes Rate = 3.30264 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.21.44_tomo_01_041_30.0.tif Record image shifted -0.003, 0.003 um Tracking reference shifted by 10.4,7.7 pixels, -0.003,0.003 um Saved Z = 41 at 27.68 min Tilt = 30.00 Mean = 97 Exp = 2.075 X/Y/Z = 0.271 -0.833 0.866 Errors in X/Y Prediction -0.022 -0.087 um, 1.7 6.6 % Error in Z Prediction 0.082 Predicted X = 0.265 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -1.028 StdErr = 0.054 nFit = 5 nDrop = 0 Predicted Z = 0.808 StdErr = 0.039 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.009, -0.143 um; position 0.273, -0.885 Drift reached 2.0 A/sec after 6.2 sec, going on Running script # 14 1.6 0.7 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.3 0.5 nm on specimen axes Rate = 3.03074 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.22.18_tomo_01_042_32.0.tif Record image shifted -0.002, 0.014 um Tracking reference shifted by 41.0,1.4 pixels, -0.002,0.014 um Saved Z = 42 at 28.25 min Tilt = 32.00 Mean = 96 Exp = 2.149 X/Y/Z = 0.276 -0.901 0.808 Errors in X/Y Prediction 0.011 0.128 um, 0.9 9.8 % Predicted X = 0.296 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = -0.145 StdErr = 0.056 nFit = 6 nDrop = 0 Predicted Z = 4.046 StdErr = 0.033 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.078 um; position 0.295, -0.223 Drift reached 3.7 A/sec after 7.1 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.20 nm/sec Tracking image shifted 0.003, 0.011 um; position 0.293, -0.237 Running script # 14 4.1 -0.5 unbinned pixels; 0.6 1.3 nm along two shift axes; 0.1 1.4 nm on specimen axes Rate = 7.16904 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.23.12_tomo_01_043_-54.0.tif Record image shifted -0.006, -0.015 um Tracking reference shifted by -41.6,21.3 pixels, -0.006,-0.015 um Saved Z = 43 at 29.18 min Tilt = -54.00 Mean = 93 Exp = 2.866 X/Y/Z = 0.299 -0.223 3.996 Errors in X/Y Prediction 0.003 -0.078 um, 0.2 6.0 % Error in Z Prediction -0.050 Predicted X = 0.297 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.364 StdErr = 0.017 nFit = 5 nDrop = 0 Predicted Z = 4.094 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.077 um; position 0.292, -0.288 Drift reached 2.5 A/sec after 7.4 sec, going on Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.10 nm/sec Tracking image shifted 0.001, 0.005 um; position 0.292, -0.295 Running script # 14 2.2 -0.9 unbinned pixels; 0.6 0.6 nm along two shift axes; 0.3 0.8 nm on specimen axes Rate = 4.2009 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.24.04_tomo_01_044_-56.0.tif Record image shifted -0.003, -0.014 um Tracking reference shifted by -40.2,12.2 pixels, -0.003,-0.014 um Saved Z = 44 at 30.04 min Tilt = -56.00 Mean = 93 Exp = 3.076 X/Y/Z = 0.295 -0.281 4.014 Errors in X/Y Prediction -0.002 0.083 um, 0.2 6.4 % Error in Z Prediction -0.079 Predicted X = 0.292 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.399 StdErr = 0.057 nFit = 5 nDrop = 0 Predicted Z = 4.103 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, -0.060 um; position 0.286, -0.339 Drift reached 2.0 A/sec after 7.7 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.12 nm/sec Tracking image shifted 0.002, 0.004 um; position 0.284, -0.344 Running script # 14 1.9 -1.1 unbinned pixels; 0.6 0.5 nm along two shift axes; 0.3 0.7 nm on specimen axes Rate = 3.74837 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.24.56_tomo_01_045_-58.0.tif Record image shifted -0.003, -0.015 um Tracking reference shifted by -42.5,11.8 pixels, -0.003,-0.015 um Saved Z = 45 at 30.91 min Tilt = -58.00 Mean = 92 Exp = 3.325 X/Y/Z = 0.286 -0.330 4.089 Errors in X/Y Prediction -0.006 0.069 um, 0.5 5.3 % Error in Z Prediction -0.015 Predicted X = 0.275 StdErr = 0.000 nFit = 4 nDrop = 0 Predicted Y = -0.366 StdErr = 0.036 nFit = 5 nDrop = 1 Predicted Z = 4.142 StdErr = 0.028 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.010, 0.028 um; position 0.285, -0.395 Drift reached 2.7 A/sec after 8.1 sec, going on Running script # 14 4.9 -1.2 unbinned pixels; 1.0 1.5 nm along two shift axes; 0.3 1.8 nm on specimen axes Rate = 8.90039 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 30 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.25.33_tomo_01_046_-60.0.tif Trimming A by 185 185, reference by 20 20 Record image shifted -0.000, -0.000 um Tracking reference shifted by -0.5,1.4 pixels, -0.000,-0.000 um Saved Z = 46 at 31.53 min Tilt = -60.00 Mean = 74 Exp = 3.625 X/Y/Z = 0.285 -0.398 4.142 Errors in X/Y Prediction 0.010 -0.032 um, 0.8 2.5 % Predicted X = 0.282 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Y = -0.431 StdErr = 0.030 nFit = 6 nDrop = 0 Predicted Z = 4.197 StdErr = 0.032 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.048 um; position 0.276, -0.479 Trimming A by 23 255 140 20, reference by 23 255 140 20 Drift reached 1.3 A/sec after 9.7 sec, going on Measured defocus = -30.29 microns changed by 16.00 drift = 0.06 nm/sec Measured defocus = 11.99 microns changed by -15.99 to target drift = 0.09 nm/sec Errors in X/Y Prediction 0.003 0.033 um, 0.2 2.5 % Need regular tracking - Need autofocus Tracking image shifted 0.029, 0.081 um; position 0.248, -0.982 Drift reached 5.8 A/sec after 6.4 sec, going on Measured defocus = -3.73 microns changed by -0.27 to target drift = 0.38 nm/sec Tracking image shifted -0.007, -0.030 um; position 0.257, -0.948 Running script # 14 -4.1 3.6 unbinned pixels; 1.7 0.9 nm along two shift axes; -1.1 -1.6 nm on specimen axes Rate = 9.54673 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.27.39_tomo_01_047_34.0.tif Record image shifted -0.014, -0.000 um Tracking reference shifted by 2.9,39.3 pixels, -0.014,-0.000 um Saved Z = 47 at 33.61 min Tilt = 34.00 Mean = 95 Exp = 2.230 X/Y/Z = 0.272 -0.947 0.516 Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.141 um; position 0.266, -1.087 Drift reached 3.2 A/sec after 6.5 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.18 nm/sec Tracking image shifted 0.001, 0.008 um; position 0.266, -1.097 Running script # 14 1.6 0.5 unbinned pixels; 0.0 0.6 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.98714 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.28.28_tomo_01_048_36.0.tif Record image shifted -0.000, 0.013 um Tracking reference shifted by 37.5,-2.2 pixels, -0.000,0.013 um Saved Z = 48 at 34.50 min Tilt = 36.00 Mean = 96 Exp = 2.347 X/Y/Z = 0.266 -1.111 0.537 Predicted X = 0.261 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -1.275 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 0.558 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.010, -0.081 um; position 0.251, -1.194 Drift reached 1.9 A/sec after 6.6 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.17 nm/sec Tracking image shifted -0.003, -0.009 um; position 0.255, -1.183 Running script # 14 -5.3 3.6 unbinned pixels; 1.8 1.3 nm along two shift axes; -1.1 -2.0 nm on specimen axes Rate = 11.2531 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.29.22_tomo_01_049_38.0.tif Record image shifted -0.002, 0.005 um Tracking reference shifted by 13.6,3.3 pixels, -0.002,0.005 um Saved Z = 49 at 35.32 min Tilt = 38.00 Mean = 95 Exp = 2.435 X/Y/Z = 0.258 -1.186 0.506 Errors in X/Y Prediction -0.003 0.089 um, 0.3 6.8 % Error in Z Prediction -0.052 Predicted X = 0.251 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -1.320 StdErr = 0.066 nFit = 3 nDrop = 0 Predicted Z = 0.510 StdErr = 0.039 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.007, -0.079 um; position 0.258, -1.241 Drift reached 2.1 A/sec after 6.7 sec, going on Running script # 14 2.1 1.4 unbinned pixels; 0.2 0.9 nm along two shift axes; -0.6 0.7 nm on specimen axes Rate = 4.44742 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.29.56_tomo_01_050_40.0.tif Record image shifted -0.003, 0.015 um Tracking reference shifted by 42.6,4.0 pixels, -0.003,0.015 um Saved Z = 50 at 35.90 min Tilt = 40.00 Mean = 94 Exp = 2.566 X/Y/Z = 0.262 -1.258 0.510 Errors in X/Y Prediction 0.011 0.063 um, 0.8 4.8 % Predicted X = 0.258 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -1.332 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Z = 0.504 StdErr = 0.052 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.002 um; position 0.257, -1.334 Drift reached 4.6 A/sec after 6.9 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.31 nm/sec Tracking image shifted -0.005, -0.013 um; position 0.264, -1.318 Running script # 14 -5.0 2.4 unbinned pixels; 1.4 1.4 nm along two shift axes; -0.7 -1.8 nm on specimen axes Rate = 9.75723 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.30.47_tomo_01_051_42.0.tif Record image shifted -0.001, 0.003 um Tracking reference shifted by 7.5,3.3 pixels, -0.001,0.003 um Saved Z = 51 at 36.75 min Tilt = 42.00 Mean = 94 Exp = 2.718 X/Y/Z = 0.266 -1.319 0.454 Errors in X/Y Prediction 0.008 0.013 um, 0.6 1.0 % Error in Z Prediction -0.050 Predicted X = 0.270 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -1.387 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Z = 0.426 StdErr = 0.005 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.010, 0.013 um; position 0.260, -1.400 Drift reached 1.6 A/sec after 7.3 sec, going on Running script # 14 1.9 1.4 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.5 0.6 nm on specimen axes Rate = 4.14814 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.31.24_tomo_01_052_44.0.tif Record image shifted -0.002, 0.017 um Tracking reference shifted by 48.3,1.3 pixels, -0.002,0.017 um Saved Z = 52 at 37.36 min Tilt = 44.00 Mean = 93 Exp = 2.896 X/Y/Z = 0.263 -1.419 0.426 Errors in X/Y Prediction -0.007 -0.032 um, 0.6 2.5 % Predicted X = 0.265 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -1.521 StdErr = 0.033 nFit = 4 nDrop = 0 Predicted Z = 0.399 StdErr = 0.006 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, -0.013 um; position 0.258, -1.507 Drift reached 5.0 A/sec after 7.5 sec, going on Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.26 nm/sec Tracking image shifted -0.006, -0.013 um; position 0.266, -1.491 Running script # 14 -1.4 3.3 unbinned pixels; 1.3 0.1 nm along two shift axes; -1.1 -0.6 nm on specimen axes Rate = 6.35753 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.32.17_tomo_01_053_46.0.tif Record image shifted -0.001, 0.004 um Tracking reference shifted by 12.3,3.1 pixels, -0.001,0.004 um Saved Z = 53 at 38.25 min Tilt = 46.00 Mean = 93 Exp = 3.106 X/Y/Z = 0.269 -1.494 0.460 Errors in X/Y Prediction 0.004 0.026 um, 0.3 2.0 % Error in Z Prediction 0.061 Predicted X = 0.269 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -1.592 StdErr = 0.024 nFit = 5 nDrop = 0 Predicted Z = 0.439 StdErr = 0.036 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.008, -0.013 um; position 0.261, -1.579 Drift reached 0.8 A/sec after 7.7 sec, going on Running script # 14 -2.5 0.6 unbinned pixels; 0.5 0.7 nm along two shift axes; -0.1 -0.9 nm on specimen axes Rate = 4.4313 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.33.00_tomo_01_054_48.0.tif Record image shifted -0.003, 0.017 um Tracking reference shifted by 47.5,4.1 pixels, -0.003,0.017 um Saved Z = 54 at 38.98 min Tilt = 48.00 Mean = 92 Exp = 3.351 X/Y/Z = 0.265 -1.595 0.439 2021/01/12 11:33 D:\Users\Ben\210112_Titan2_Jans_cells\SEM\tomo_01.st 0 55 3838 3710 1566 -10.0 48.0 2.00 REG 39.0 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Measured defocus = -6.66 microns changed by 2.66 to target drift = 0.11 nm/sec Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = -0.000, -0.000 Trimming A by 21 145, reference by 21 145 Angle = 18.00, shift = 8.5, 9.0 pixels, 0.018, 0.022 um, IS = -0.018, -0.022 Trimming A by 21 145, reference by 21 145 Angle = 11.00, shift = -69.0, 0.2 pixels, 0.018, -0.157 um, IS = -0.036, 0.135 Trimming A by 21 145, reference by 21 145 Angle = 4.00, shift = -40.3, -0.1 pixels, 0.010, -0.092 um, IS = -0.046, 0.226 Trimming A by 20 24 20 140, reference by 20 24 20 140 Angle = -4.00, shift = -6.2, -11.7 pixels, -0.025, -0.017 um, IS = -0.021, 0.244 Trimming A by 20 140, reference by 20 140 Angle = -11.00, shift = 1.7, -10.2 pixels, -0.024, 0.001 um, IS = 0.002, 0.242 Trimming A by 21 145, reference by 20 140 Angle = -18.00, shift = 54.0, 11.3 pixels, 0.012, 0.125 um, IS = -0.009, 0.117 Trimming A by 21 145, reference by 21 145 Angle = -24.00, shift = 38.9, 12.0 pixels, 0.017, 0.091 um, IS = -0.027, 0.026 General solution delZ 0.12, delY 2.55, yZero 0.23 solution with assumed YZero 0.23: delZ 0.12, delY 2.63 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.12 microns and lateral displacement of -2.55 microns Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.000 um; position -0.000, -0.000 Drift reached 0.0 A/sec after 5.5 sec, going on Measured defocus = -3.69 microns changed by -0.31 to target drift = 0.01 nm/sec Tracking image shifted 0.000, -0.000 um; position -0.001, -0.000 Running script # 14 -0.2 -0.2 unbinned pixels; 0.1 0.1 nm along two shift axes; 0.1 -0.1 nm on specimen axes Rate = 0.51783 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.42.57_tomo_02_000_-10.0.tif Record image shifted 0.001, -0.000 um Tracking reference shifted by -1.4,-2.0 pixels, 0.001,-0.000 um Saved Z = 0 at 0.00 min Tilt = -10.00 Mean = 80 Exp = 1.680 X/Y/Z = -0.001 0.000 2.798 Need regular tracking - Need autofocus Tracking image shifted -0.027, -0.160 um; position 0.026, 0.160 Drift reached 1.6 A/sec after 5.7 sec, going on Measured defocus = -4.11 microns changed by 0.11 to target drift = 0.12 nm/sec Tracking image shifted -0.001, -0.004 um; position 0.027, 0.166 Running script # 14 -1.6 0.1 unbinned pixels; 0.2 0.5 nm along two shift axes; 0.0 -0.6 nm on specimen axes Rate = 2.7798 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.43.39_tomo_02_001_-12.0.tif Record image shifted -0.001, -0.003 um Tracking reference shifted by -6.9,3.9 pixels, -0.001,-0.003 um Saved Z = 1 at 0.70 min Tilt = -12.00 Mean = 85 Exp = 1.788 X/Y/Z = 0.028 0.169 2.911 Predicted X = 0.058 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.337 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.024 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.022, 0.167 um; position 0.036, 0.170 Drift reached 4.0 A/sec after 5.6 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.18 nm/sec Tracking image shifted 0.005, 0.012 um; position 0.032, 0.156 Running script # 14 2.6 -0.4 unbinned pixels; 0.4 0.8 nm along two shift axes; 0.0 0.9 nm on specimen axes Rate = 4.66681 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.44.21_tomo_02_002_-14.0.tif Record image shifted 0.000, 0.008 um Tracking reference shifted by 21.2,-3.1 pixels, 0.000,0.008 um Saved Z = 2 at 1.40 min Tilt = -14.00 Mean = 84 Exp = 1.793 X/Y/Z = 0.031 0.147 3.008 Errors in X/Y Prediction -0.027 -0.190 um, 2.0 14.6 % Error in Z Prediction -0.017 Need regular tracking - Need autofocus Tracking image shifted -0.038, -0.227 um; position 0.037, 0.227 Drift reached 1.1 A/sec after 5.6 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.08 nm/sec Tracking image shifted -0.003, -0.003 um; position 0.040, 0.231 Running script # 14 -1.3 0.3 unbinned pixels; 0.3 0.4 nm along two shift axes; -0.1 -0.5 nm on specimen axes Rate = 2.39134 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.45.06_tomo_02_003_-8.0.tif Record image shifted -0.001, -0.003 um Tracking reference shifted by -6.9,4.9 pixels, -0.001,-0.003 um Saved Z = 3 at 2.23 min Tilt = -8.00 Mean = 85 Exp = 1.788 X/Y/Z = 0.041 0.234 2.764 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.014 um; position 0.043, 0.248 Drift reached 3.0 A/sec after 5.7 sec, going on Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.11 nm/sec Tracking image shifted 0.000, 0.006 um; position 0.043, 0.240 Running script # 14 1.1 -0.2 unbinned pixels; 0.2 0.3 nm along two shift axes; 0.0 0.4 nm on specimen axes Rate = 1.93568 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.45.56_tomo_02_004_-6.0.tif Record image shifted 0.000, 0.002 um Tracking reference shifted by 5.7,-0.8 pixels, 0.000,0.002 um Saved Z = 4 at 2.97 min Tilt = -6.00 Mean = 85 Exp = 1.780 X/Y/Z = 0.043 0.237 2.684 Predicted X = 0.052 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Y = 0.252 StdErr = 0.142 nFit = 3 nDrop = 0 Predicted Z = 3.116 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.009, 0.174 um; position 0.044, 0.078 Drift reached 1.2 A/sec after 5.6 sec, going on Running script # 14 -0.9 0.5 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.2 -0.3 nm on specimen axes Rate = 1.76149 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.46.27_tomo_02_005_-16.0.tif Record image shifted 0.003, 0.002 um Tracking reference shifted by 3.7,-10.3 pixels, 0.003,0.002 um Saved Z = 5 at 3.49 min Tilt = -16.00 Mean = 84 Exp = 1.806 X/Y/Z = 0.041 0.078 3.116 Errors in X/Y Prediction -0.011 -0.175 um, 0.9 13.4 % Predicted X = 0.046 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = 0.040 StdErr = 0.036 nFit = 3 nDrop = 0 Predicted Z = 3.221 StdErr = 0.017 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, -0.013 um; position 0.040, 0.053 Drift reached 3.1 A/sec after 5.7 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.14 nm/sec Tracking image shifted 0.001, 0.007 um; position 0.039, 0.043 Running script # 14 1.8 -0.6 unbinned pixels; 0.4 0.5 nm along two shift axes; 0.2 0.6 nm on specimen axes Rate = 3.28929 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.47.09_tomo_02_006_-18.0.tif Record image shifted -0.004, -0.002 um Tracking reference shifted by -4.3,12.4 pixels, -0.004,-0.002 um Saved Z = 6 at 4.20 min Tilt = -18.00 Mean = 84 Exp = 1.830 X/Y/Z = 0.043 0.045 3.181 Errors in X/Y Prediction -0.004 0.005 um, 0.3 0.4 % Error in Z Prediction -0.039 Predicted X = 0.044 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.241 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 2.604 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, -0.031 um; position 0.042, 0.271 Drift reached 0.9 A/sec after 5.7 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.10 nm/sec Tracking image shifted 0.000, -0.005 um; position 0.042, 0.277 Running script # 14 -1.2 0.0 unbinned pixels; 0.1 0.4 nm along two shift axes; 0.0 -0.4 nm on specimen axes Rate = 2.16891 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.47.55_tomo_02_007_-4.0.tif Record image shifted 0.000, -0.002 um Tracking reference shifted by -6.2,0.5 pixels, 0.000,-0.002 um Saved Z = 7 at 4.97 min Tilt = -4.00 Mean = 85 Exp = 1.781 X/Y/Z = 0.042 0.280 2.622 Errors in X/Y Prediction -0.002 0.039 um, 0.1 3.0 % Error in Z Prediction 0.018 Predicted X = 0.043 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.296 StdErr = 0.029 nFit = 3 nDrop = 0 Predicted Z = 2.548 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, -0.019 um; position 0.044, 0.315 Drift reached 3.2 A/sec after 5.7 sec, going on Running script # 14 4.0 -0.4 unbinned pixels; 0.6 1.3 nm along two shift axes; 0.0 1.4 nm on specimen axes Rate = 7.00713 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.48.27_tomo_02_008_-2.0.tif Record image shifted -0.001, 0.006 um Tracking reference shifted by 15.9,0.2 pixels, -0.001,0.006 um Saved Z = 8 at 5.50 min Tilt = -2.00 Mean = 85 Exp = 1.781 X/Y/Z = 0.044 0.306 2.548 Errors in X/Y Prediction 0.001 0.010 um, 0.1 0.8 % Predicted X = 0.049 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.013 StdErr = 0.027 nFit = 3 nDrop = 0 Predicted Z = 3.272 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.009, 0.044 um; position 0.040, -0.057 Drift reached 0.5 A/sec after 5.7 sec, going on Running script # 14 -0.1 0.2 unbinned pixels; 0.1 0.0 nm along two shift axes; -0.1 -0.0 nm on specimen axes Rate = 0.39784 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.48.59_tomo_02_009_-20.0.tif Record image shifted 0.001, 0.001 um Tracking reference shifted by 3.3,-3.2 pixels, 0.001,0.001 um Saved Z = 9 at 6.04 min Tilt = -20.00 Mean = 84 Exp = 1.850 X/Y/Z = 0.039 -0.058 3.272 Errors in X/Y Prediction -0.010 -0.046 um, 0.8 3.5 % Predicted X = 0.039 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.151 StdErr = 0.071 nFit = 4 nDrop = 0 Predicted Z = 3.362 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.100 um; position 0.040, -0.051 Drift reached 3.6 A/sec after 5.7 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.16 nm/sec Tracking image shifted 0.000, 0.007 um; position 0.041, -0.060 Running script # 14 1.9 -0.3 unbinned pixels; 0.3 0.6 nm along two shift axes; 0.1 0.7 nm on specimen axes Rate = 3.36572 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.49.42_tomo_02_010_-22.0.tif Record image shifted -0.001, 0.002 um Tracking reference shifted by 5.1,3.8 pixels, -0.001,0.002 um Saved Z = 10 at 6.74 min Tilt = -22.00 Mean = 84 Exp = 1.877 X/Y/Z = 0.042 -0.062 3.330 Errors in X/Y Prediction 0.002 0.089 um, 0.2 6.8 % Error in Z Prediction -0.032 Predicted X = 0.045 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.342 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Z = 2.477 StdErr = 0.018 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.048 um; position 0.044, 0.294 Drift reached 1.5 A/sec after 5.7 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.12 nm/sec Tracking image shifted 0.000, -0.006 um; position 0.044, 0.301 Running script # 14 -1.5 0.2 unbinned pixels; 0.2 0.5 nm along two shift axes; -0.0 -0.5 nm on specimen axes Rate = 2.69701 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.50.28_tomo_02_011_0.0.tif Record image shifted 0.000, -0.004 um Tracking reference shifted by -10.9,0.9 pixels, 0.000,-0.004 um Saved Z = 11 at 7.52 min Tilt = 0.00 Mean = 85 Exp = 1.791 X/Y/Z = 0.044 0.306 2.492 Errors in X/Y Prediction -0.001 -0.037 um, 0.1 2.8 % Error in Z Prediction 0.015 Predicted X = 0.045 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.323 StdErr = 0.020 nFit = 3 nDrop = 0 Predicted Z = 2.428 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, -0.008 um; position 0.042, 0.331 Drift reached 3.1 A/sec after 5.7 sec, going on Running script # 14 3.6 -0.3 unbinned pixels; 0.5 1.2 nm along two shift axes; -0.0 1.3 nm on specimen axes Rate = 6.39795 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.51.01_tomo_02_012_2.0.tif Record image shifted -0.001, 0.007 um Tracking reference shifted by 19.6,-0.2 pixels, -0.001,0.007 um Saved Z = 12 at 8.06 min Tilt = 2.00 Mean = 85 Exp = 1.801 X/Y/Z = 0.043 0.321 2.428 Errors in X/Y Prediction -0.002 -0.002 um, 0.2 0.2 % Predicted X = 0.041 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.106 StdErr = 0.064 nFit = 5 nDrop = 0 Predicted Z = 3.414 StdErr = 0.016 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.000, -0.004 um; position 0.041, -0.102 Drift reached 0.7 A/sec after 5.7 sec, going on Running script # 14 0.6 -0.2 unbinned pixels; 0.1 0.2 nm along two shift axes; 0.0 0.2 nm on specimen axes Rate = 1.12352 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.51.33_tomo_02_013_-24.0.tif Record image shifted -0.001, 0.002 um Tracking reference shifted by 6.5,0.9 pixels, -0.001,0.002 um Saved Z = 13 at 8.60 min Tilt = -24.00 Mean = 84 Exp = 1.904 X/Y/Z = 0.041 -0.105 3.414 Errors in X/Y Prediction 0.000 0.001 um, 0.0 0.1 % Predicted X = 0.043 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = -0.123 StdErr = 0.063 nFit = 5 nDrop = 0 Predicted Z = 3.495 StdErr = 0.019 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.003 um; position 0.043, -0.125 Drift reached 2.9 A/sec after 5.9 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.16 nm/sec Tracking image shifted 0.000, 0.007 um; position 0.043, -0.134 Running script # 14 1.8 -0.3 unbinned pixels; 0.3 0.5 nm along two shift axes; 0.0 0.6 nm on specimen axes Rate = 3.13179 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.52.16_tomo_02_014_-26.0.tif Record image shifted -0.000, 0.000 um Tracking reference shifted by 0.1,0.6 pixels, -0.000,0.000 um Saved Z = 14 at 9.32 min Tilt = -26.00 Mean = 83 Exp = 1.935 X/Y/Z = 0.043 -0.135 3.509 Errors in X/Y Prediction 0.000 -0.012 um, 0.0 1.0 % Error in Z Prediction 0.014 Predicted X = 0.040 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = 0.320 StdErr = 0.028 nFit = 4 nDrop = 0 Predicted Z = 2.364 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.006, 0.010 um; position 0.045, 0.310 Drift reached 2.6 A/sec after 5.7 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.15 nm/sec Tracking image shifted -0.001, -0.006 um; position 0.046, 0.318 Running script # 14 -1.7 0.5 unbinned pixels; 0.4 0.5 nm along two shift axes; -0.1 -0.6 nm on specimen axes Rate = 3.13742 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.53.04_tomo_02_015_4.0.tif Record image shifted -0.000, -0.005 um Tracking reference shifted by -13.7,2.3 pixels, -0.000,-0.005 um Saved Z = 15 at 10.11 min Tilt = 4.00 Mean = 84 Exp = 1.810 X/Y/Z = 0.047 0.323 2.357 Errors in X/Y Prediction 0.007 0.003 um, 0.5 0.2 % Error in Z Prediction -0.006 Predicted X = 0.052 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = 0.322 StdErr = 0.016 nFit = 5 nDrop = 0 Predicted Z = 2.292 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.032 um; position 0.048, 0.354 Drift reached 3.6 A/sec after 5.7 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.14 nm/sec Tracking image shifted -0.001, 0.007 um; position 0.049, 0.345 Running script # 14 1.3 0.4 unbinned pixels; 0.0 0.5 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.37588 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.53.48_tomo_02_016_6.0.tif Record image shifted -0.000, -0.001 um Tracking reference shifted by -2.8,0.9 pixels, -0.000,-0.001 um Saved Z = 16 at 10.85 min Tilt = 6.00 Mean = 84 Exp = 1.825 X/Y/Z = 0.050 0.346 2.232 Errors in X/Y Prediction -0.002 0.024 um, 0.2 1.8 % Error in Z Prediction -0.060 Predicted X = 0.044 StdErr = 0.005 nFit = 4 nDrop = 0 Predicted Y = -0.131 StdErr = 0.059 nFit = 5 nDrop = 0 Predicted Z = 3.585 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, 0.053 um; position 0.046, -0.185 Drift reached 3.4 A/sec after 5.9 sec, going on Running script # 14 4.4 0.2 unbinned pixels; 0.5 1.5 nm along two shift axes; -0.2 1.5 nm on specimen axes Rate = 7.73445 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.54.22_tomo_02_017_-28.0.tif Record image shifted 0.001, 0.009 um Tracking reference shifted by 24.0,-4.1 pixels, 0.001,0.009 um Saved Z = 17 at 11.42 min Tilt = -28.00 Mean = 83 Exp = 1.974 X/Y/Z = 0.046 -0.197 3.585 Errors in X/Y Prediction 0.002 -0.065 um, 0.2 5.0 % Predicted X = 0.049 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -0.268 StdErr = 0.019 nFit = 5 nDrop = 0 Predicted Z = 3.667 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.081 um; position 0.045, -0.187 Drift reached 1.3 A/sec after 5.9 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.05 nm/sec Tracking image shifted 0.001, 0.012 um; position 0.045, -0.200 Running script # 14 0.1 0.1 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes Rate = 0.25894 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.55.05_tomo_02_018_-30.0.tif Record image shifted -0.001, -0.004 um Tracking reference shifted by -10.2,4.3 pixels, -0.001,-0.004 um Saved Z = 18 at 12.14 min Tilt = -30.00 Mean = 83 Exp = 2.017 X/Y/Z = 0.046 -0.196 3.619 Errors in X/Y Prediction -0.003 0.072 um, 0.2 5.5 % Error in Z Prediction -0.048 Predicted X = 0.056 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = 0.366 StdErr = 0.013 nFit = 5 nDrop = 1 Predicted Z = 2.165 StdErr = 0.049 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, 0.081 um; position 0.053, 0.285 Drift reached 2.3 A/sec after 5.7 sec, going on Running script # 14 -3.9 1.8 unbinned pixels; 1.0 1.1 nm along two shift axes; -0.5 -1.4 nm on specimen axes Rate = 7.57421 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.55.40_tomo_02_019_8.0.tif Record image shifted 0.000, -0.011 um Tracking reference shifted by -32.1,1.9 pixels, 0.000,-0.011 um Saved Z = 19 at 12.72 min Tilt = 8.00 Mean = 84 Exp = 1.841 X/Y/Z = 0.054 0.299 2.165 Errors in X/Y Prediction -0.002 -0.067 um, 0.1 5.1 % Predicted X = 0.058 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.291 StdErr = 0.035 nFit = 6 nDrop = 0 Predicted Z = 2.081 StdErr = 0.060 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, 0.017 um; position 0.051, 0.275 Drift reached 3.3 A/sec after 5.7 sec, going on Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.14 nm/sec Tracking image shifted -0.001, 0.008 um; position 0.052, 0.265 Running script # 14 1.1 0.3 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.0646 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.56.24_tomo_02_020_10.0.tif Record image shifted -0.001, 0.006 um Tracking reference shifted by 17.7,2.3 pixels, -0.001,0.006 um Saved Z = 20 at 13.45 min Tilt = 10.00 Mean = 84 Exp = 1.856 X/Y/Z = 0.053 0.259 2.132 Errors in X/Y Prediction -0.004 -0.033 um, 0.3 2.5 % Error in Z Prediction 0.050 Predicted X = 0.046 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = -0.247 StdErr = 0.038 nFit = 6 nDrop = 0 Predicted Z = 3.703 StdErr = 0.044 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.007, -0.009 um; position 0.053, -0.238 Drift reached 2.7 A/sec after 5.9 sec, going on Running script # 14 3.5 -0.1 unbinned pixels; 0.4 1.2 nm along two shift axes; -0.1 1.2 nm on specimen axes Rate = 6.21292 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.56.58_tomo_02_021_-32.0.tif Record image shifted 0.000, 0.011 um Tracking reference shifted by 30.8,-3.0 pixels, 0.000,0.011 um Saved Z = 21 at 14.02 min Tilt = -32.00 Mean = 83 Exp = 2.066 X/Y/Z = 0.053 -0.252 3.703 Errors in X/Y Prediction 0.007 -0.005 um, 0.5 0.4 % Predicted X = 0.055 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.277 StdErr = 0.044 nFit = 5 nDrop = 0 Predicted Z = 3.776 StdErr = 0.051 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.012 um; position 0.051, -0.266 Drift reached 0.2 A/sec after 6.1 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.02 nm/sec Tracking image shifted -0.001, -0.000 um; position 0.051, -0.266 Running script # 14 -0.1 0.6 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 -0.0 nm on specimen axes Rate = 1.10108 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.57.43_tomo_02_022_-34.0.tif Record image shifted -0.000, -0.004 um Tracking reference shifted by -11.0,2.3 pixels, -0.000,-0.004 um Saved Z = 22 at 14.77 min Tilt = -34.00 Mean = 82 Exp = 2.120 X/Y/Z = 0.052 -0.262 3.727 Errors in X/Y Prediction -0.003 0.016 um, 0.2 1.2 % Error in Z Prediction -0.048 Predicted X = 0.056 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.202 StdErr = 0.028 nFit = 6 nDrop = 0 Predicted Z = 2.044 StdErr = 0.042 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, -0.039 um; position 0.059, 0.241 Drift reached 2.6 A/sec after 5.7 sec, going on Running script # 14 -5.2 1.8 unbinned pixels; 1.2 1.5 nm along two shift axes; -0.5 -1.9 nm on specimen axes Rate = 9.69029 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.58.19_tomo_02_023_12.0.tif Record image shifted -0.002, -0.013 um Tracking reference shifted by -35.2,7.4 pixels, -0.002,-0.013 um Saved Z = 23 at 15.37 min Tilt = 12.00 Mean = 84 Exp = 1.888 X/Y/Z = 0.061 0.256 2.044 Errors in X/Y Prediction 0.005 0.055 um, 0.4 4.2 % Predicted X = 0.063 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = 0.209 StdErr = 0.058 nFit = 5 nDrop = 0 Predicted Z = 1.970 StdErr = 0.048 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, 0.002 um; position 0.061, 0.207 Drift reached 3.3 A/sec after 5.7 sec, going on Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.14 nm/sec Tracking image shifted -0.001, 0.007 um; position 0.062, 0.198 Running script # 14 1.2 0.3 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.1 0.4 nm on specimen axes Rate = 2.23548 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.59.04_tomo_02_024_14.0.tif Record image shifted 0.001, 0.006 um Tracking reference shifted by 15.9,-3.9 pixels, 0.001,0.006 um Saved Z = 24 at 16.11 min Tilt = 14.00 Mean = 83 Exp = 1.913 X/Y/Z = 0.061 0.192 2.011 Errors in X/Y Prediction -0.002 -0.017 um, 0.1 1.3 % Error in Z Prediction 0.041 Predicted X = 0.056 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.277 StdErr = 0.045 nFit = 5 nDrop = 0 Predicted Z = 3.782 StdErr = 0.002 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.005, 0.056 um; position 0.061, -0.333 Drift reached 3.0 A/sec after 6.1 sec, going on Running script # 14 4.1 0.4 unbinned pixels; 0.3 1.4 nm along two shift axes; -0.3 1.4 nm on specimen axes Rate = 7.28893 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_11.59.39_tomo_02_025_-36.0.tif Record image shifted 0.001, 0.010 um Tracking reference shifted by 28.2,-6.2 pixels, 0.001,0.010 um Saved Z = 25 at 16.70 min Tilt = -36.00 Mean = 82 Exp = 2.186 X/Y/Z = 0.061 -0.347 3.782 Errors in X/Y Prediction 0.004 -0.070 um, 0.3 5.3 % Predicted X = 0.063 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.423 StdErr = 0.045 nFit = 5 nDrop = 0 Predicted Z = 3.837 StdErr = 0.002 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.007, -0.039 um; position 0.056, -0.384 Drift reached 0.7 A/sec after 6.1 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.04 nm/sec Tracking image shifted -0.001, 0.000 um; position 0.057, -0.385 Running script # 14 0.4 0.5 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.12451 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.00.23_tomo_02_026_-38.0.tif Record image shifted -0.001, -0.001 um Tracking reference shifted by -2.5,3.8 pixels, -0.001,-0.001 um Saved Z = 26 at 17.43 min Tilt = -38.00 Mean = 82 Exp = 2.263 X/Y/Z = 0.059 -0.384 3.861 Errors in X/Y Prediction -0.005 0.039 um, 0.4 3.0 % Error in Z Prediction 0.023 Predicted X = 0.067 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = 0.166 StdErr = 0.045 nFit = 5 nDrop = 0 Predicted Z = 1.938 StdErr = 0.045 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, 0.024 um; position 0.063, 0.143 Drift reached 4.1 A/sec after 5.9 sec, going on Running script # 14 -8.2 1.9 unbinned pixels; 1.6 2.5 nm along two shift axes; -0.4 -2.9 nm on specimen axes Rate = 14.74607 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.00.59_tomo_02_027_16.0.tif Record image shifted -0.001, -0.015 um Tracking reference shifted by -41.0,7.1 pixels, -0.001,-0.015 um Saved Z = 27 at 18.03 min Tilt = 16.00 Mean = 83 Exp = 1.950 X/Y/Z = 0.065 0.163 1.938 Errors in X/Y Prediction -0.002 -0.004 um, 0.2 0.3 % Predicted X = 0.066 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.112 StdErr = 0.040 nFit = 5 nDrop = 0 Predicted Z = 1.872 StdErr = 0.051 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.019 um; position 0.065, 0.131 Drift reached 3.4 A/sec after 5.9 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.14 nm/sec Tracking image shifted -0.002, 0.007 um; position 0.067, 0.121 Running script # 14 1.1 0.6 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.2 0.4 nm on specimen axes Rate = 2.16113 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.01.45_tomo_02_028_18.0.tif Record image shifted 0.001, 0.008 um Tracking reference shifted by 22.3,-5.1 pixels, 0.001,0.008 um Saved Z = 28 at 18.80 min Tilt = 18.00 Mean = 83 Exp = 1.986 X/Y/Z = 0.066 0.113 1.815 Errors in X/Y Prediction 0.001 0.001 um, 0.0 0.1 % Error in Z Prediction -0.058 Predicted X = 0.064 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.448 StdErr = 0.049 nFit = 5 nDrop = 0 Predicted Z = 3.917 StdErr = 0.016 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, 0.018 um; position 0.064, -0.466 Drift reached 2.4 A/sec after 6.3 sec, going on Running script # 14 4.9 0.2 unbinned pixels; 0.5 1.7 nm along two shift axes; -0.2 1.7 nm on specimen axes Rate = 8.64051 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.02.21_tomo_02_029_-40.0.tif Record image shifted 0.000, 0.012 um Tracking reference shifted by 32.7,-3.8 pixels, 0.000,0.012 um Saved Z = 29 at 19.42 min Tilt = -40.00 Mean = 81 Exp = 2.346 X/Y/Z = 0.064 -0.481 3.917 Errors in X/Y Prediction 0.000 -0.033 um, 0.0 2.5 % Predicted X = 0.064 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.583 StdErr = 0.047 nFit = 5 nDrop = 0 Predicted Z = 3.977 StdErr = 0.019 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.056 um; position 0.059, -0.526 Drift reached 1.1 A/sec after 6.5 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Tracking image shifted -0.001, 0.002 um; position 0.061, -0.529 Running script # 14 1.0 0.4 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.94186 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.03.08_tomo_02_030_-42.0.tif Record image shifted -0.001, -0.000 um Tracking reference shifted by -0.4,2.2 pixels, -0.001,-0.000 um Saved Z = 30 at 20.19 min Tilt = -42.00 Mean = 81 Exp = 2.451 X/Y/Z = 0.062 -0.529 3.970 Errors in X/Y Prediction -0.003 0.054 um, 0.2 4.1 % Error in Z Prediction -0.007 Predicted X = 0.063 StdErr = 0.010 nFit = 4 nDrop = 0 Predicted Y = -0.594 StdErr = 0.043 nFit = 5 nDrop = 0 Predicted Z = 4.035 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.049 um; position 0.062, -0.545 Drift reached 3.2 A/sec after 6.5 sec, going on Running script # 14 5.7 0.1 unbinned pixels; 0.6 1.9 nm along two shift axes; -0.2 2.0 nm on specimen axes Rate = 10.0428 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.03.40_tomo_02_031_-44.0.tif Record image shifted 0.000, 0.011 um Tracking reference shifted by 31.1,-4.1 pixels, 0.000,0.011 um Saved Z = 31 at 20.72 min Tilt = -44.00 Mean = 81 Exp = 2.558 X/Y/Z = 0.062 -0.560 4.035 Errors in X/Y Prediction -0.001 0.034 um, 0.1 2.6 % Predicted X = 0.069 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.051 StdErr = 0.038 nFit = 5 nDrop = 0 Predicted Z = 1.748 StdErr = 0.048 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.066 um; position 0.068, 0.117 Drift reached 5.5 A/sec after 5.9 sec, going on Running script # 14 -10.2 3.0 unbinned pixels; 2.2 3.0 nm along two shift axes; -0.8 -3.7 nm on specimen axes Rate = 18.64151 A/sec -7.9 2.8 unbinned pixels; 1.8 2.3 nm along two shift axes; -0.7 -2.8 nm on specimen axes Rate = 14.59181 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.04.23_tomo_02_032_20.0.tif Record image shifted -0.002, -0.017 um Tracking reference shifted by -48.9,10.6 pixels, -0.002,-0.017 um Saved Z = 32 at 21.43 min Tilt = 20.00 Mean = 83 Exp = 2.038 X/Y/Z = 0.073 0.144 1.748 Errors in X/Y Prediction 0.003 0.093 um, 0.3 7.1 % Predicted X = 0.076 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.168 StdErr = 0.040 nFit = 5 nDrop = 0 Predicted Z = 1.669 StdErr = 0.056 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.124 um; position 0.071, 0.044 Drift reached 3.0 A/sec after 5.9 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.12 nm/sec Tracking image shifted -0.000, -0.008 um; position 0.072, 0.050 Running script # 14 1.3 0.2 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.1 0.5 nm on specimen axes Rate = 2.34109 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.05.09_tomo_02_033_22.0.tif Record image shifted 0.001, 0.010 um Tracking reference shifted by 27.4,-4.0 pixels, 0.001,0.010 um Saved Z = 33 at 22.20 min Tilt = 22.00 Mean = 82 Exp = 2.083 X/Y/Z = 0.071 0.040 1.651 Errors in X/Y Prediction -0.004 -0.128 um, 0.3 9.8 % Error in Z Prediction -0.018 Predicted X = 0.075 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.001 StdErr = 0.088 nFit = 5 nDrop = 0 Predicted Z = 1.555 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.017, 0.026 um; position 0.058, -0.027 Drift reached 3.4 A/sec after 6.1 sec, going on Running script # 14 -6.2 1.6 unbinned pixels; 1.3 1.9 nm along two shift axes; -0.4 -2.2 nm on specimen axes Rate = 11.20584 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.05.42_tomo_02_034_24.0.tif Record image shifted -0.001, -0.017 um Tracking reference shifted by -48.5,6.5 pixels, -0.001,-0.017 um Saved Z = 34 at 22.76 min Tilt = 24.00 Mean = 83 Exp = 2.150 X/Y/Z = 0.060 -0.006 1.555 Errors in X/Y Prediction -0.015 -0.005 um, 1.2 0.4 % Predicted X = 0.061 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.600 StdErr = 0.050 nFit = 5 nDrop = 0 Predicted Z = 4.095 StdErr = 0.018 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.091 um; position 0.059, -0.692 Drift reached 4.1 A/sec after 6.7 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.22 nm/sec Tracking image shifted 0.001, 0.012 um; position 0.059, -0.706 Running script # 14 4.6 0.5 unbinned pixels; 0.4 1.6 nm along two shift axes; -0.3 1.6 nm on specimen axes Rate = 8.15897 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.06.35_tomo_02_035_-46.0.tif Record image shifted 0.000, 0.010 um Tracking reference shifted by 29.1,-3.1 pixels, 0.000,0.010 um Saved Z = 35 at 23.64 min Tilt = -46.00 Mean = 80 Exp = 2.693 X/Y/Z = 0.059 -0.718 4.086 Errors in X/Y Prediction -0.002 -0.118 um, 0.1 9.0 % Error in Z Prediction -0.010 Predicted X = 0.058 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.811 StdErr = 0.096 nFit = 5 nDrop = 0 Predicted Z = 4.141 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, -0.036 um; position 0.054, -0.775 Drift reached 1.4 A/sec after 6.9 sec, going on Running script # 14 2.3 -0.0 unbinned pixels; 0.3 0.7 nm along two shift axes; -0.1 0.8 nm on specimen axes Rate = 3.9585 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.07.08_tomo_02_036_-48.0.tif Record image shifted -0.002, 0.008 um Tracking reference shifted by 24.3,2.5 pixels, -0.002,0.008 um Saved Z = 36 at 24.20 min Tilt = -48.00 Mean = 80 Exp = 2.846 X/Y/Z = 0.056 -0.785 4.141 Errors in X/Y Prediction -0.002 0.026 um, 0.2 2.0 % Predicted X = 0.053 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -0.937 StdErr = 0.083 nFit = 5 nDrop = 0 Predicted Z = 4.197 StdErr = 0.005 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, -0.123 um; position 0.058, -0.814 Drift reached 2.4 A/sec after 7.1 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.13 nm/sec Tracking image shifted -0.001, 0.008 um; position 0.059, -0.823 Running script # 14 2.8 -0.2 unbinned pixels; 0.4 0.9 nm along two shift axes; -0.0 1.0 nm on specimen axes Rate = 4.96561 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.07.59_tomo_02_037_-50.0.tif Record image shifted 0.002, 0.012 um Tracking reference shifted by 33.4,-8.0 pixels, 0.002,0.012 um Saved Z = 37 at 25.05 min Tilt = -50.00 Mean = 80 Exp = 3.015 X/Y/Z = 0.058 -0.836 4.156 Errors in X/Y Prediction 0.005 0.100 um, 0.4 7.7 % Error in Z Prediction -0.041 Predicted X = 0.056 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.912 StdErr = 0.096 nFit = 5 nDrop = 0 Predicted Z = 4.210 StdErr = 0.029 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, 0.003 um; position 0.053, -0.915 Drift reached 1.0 A/sec after 7.3 sec, going on Running script # 14 2.5 -0.2 unbinned pixels; 0.4 0.8 nm along two shift axes; -0.0 0.9 nm on specimen axes Rate = 4.35391 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.08.34_tomo_02_038_-52.0.tif Record image shifted -0.000, 0.012 um Tracking reference shifted by 34.3,-1.9 pixels, -0.000,0.012 um Saved Z = 38 at 25.64 min Tilt = -52.00 Mean = 79 Exp = 3.222 X/Y/Z = 0.054 -0.929 4.210 Errors in X/Y Prediction -0.002 -0.017 um, 0.2 1.3 % Predicted X = 0.055 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.096 StdErr = 0.083 nFit = 5 nDrop = 0 Predicted Z = 1.465 StdErr = 0.024 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.011, -0.066 um; position 0.066, -0.030 Drift reached 4.3 A/sec after 6.1 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.26 nm/sec Tracking image shifted -0.003, -0.012 um; position 0.071, -0.016 Running script # 14 -4.7 1.0 unbinned pixels; 0.9 1.4 nm along two shift axes; -0.2 -1.7 nm on specimen axes Rate = 8.37898 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.09.27_tomo_02_039_26.0.tif Record image shifted -0.003, -0.012 um Tracking reference shifted by -32.8,10.4 pixels, -0.003,-0.012 um Saved Z = 39 at 26.50 min Tilt = 26.00 Mean = 82 Exp = 2.212 X/Y/Z = 0.073 -0.002 1.409 Errors in X/Y Prediction 0.018 0.094 um, 1.4 7.2 % Error in Z Prediction -0.056 Predicted X = 0.070 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = -0.054 StdErr = 0.106 nFit = 5 nDrop = 0 Predicted Z = 1.321 StdErr = 0.050 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, 0.045 um; position 0.069, -0.100 Drift reached 2.5 A/sec after 6.3 sec, going on Running script # 14 1.9 -0.0 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.1 0.7 nm on specimen axes Rate = 3.41857 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.10.01_tomo_02_040_28.0.tif Record image shifted 0.001, -0.005 um Tracking reference shifted by -13.3,-0.6 pixels, 0.001,-0.005 um Saved Z = 40 at 27.08 min Tilt = 28.00 Mean = 82 Exp = 2.292 X/Y/Z = 0.069 -0.097 1.321 Errors in X/Y Prediction -0.002 -0.043 um, 0.1 3.3 % Predicted X = 0.076 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = -0.108 StdErr = 0.088 nFit = 5 nDrop = 0 Predicted Z = 1.219 StdErr = 0.058 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.018, 0.058 um; position 0.058, -0.167 Drift reached 2.6 A/sec after 6.3 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.13 nm/sec Tracking image shifted -0.000, -0.007 um; position 0.059, -0.158 Running script # 14 -2.1 0.9 unbinned pixels; 0.5 0.6 nm along two shift axes; -0.3 -0.8 nm on specimen axes Rate = 3.98771 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.10.49_tomo_02_041_30.0.tif Record image shifted -0.001, -0.018 um Tracking reference shifted by -51.9,7.0 pixels, -0.001,-0.018 um Saved Z = 41 at 27.88 min Tilt = 30.00 Mean = 82 Exp = 2.377 X/Y/Z = 0.061 -0.139 1.245 Errors in X/Y Prediction -0.016 -0.031 um, 1.2 2.4 % Error in Z Prediction 0.026 Predicted X = 0.055 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.222 StdErr = 0.060 nFit = 5 nDrop = 0 Predicted Z = 1.131 StdErr = 0.050 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.009, -0.034 um; position 0.064, -0.187 Drift reached 1.9 A/sec after 6.5 sec, going on Running script # 14 1.5 0.3 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.76068 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.11.24_tomo_02_042_32.0.tif Record image shifted -0.002, -0.007 um Tracking reference shifted by -19.6,6.0 pixels, -0.002,-0.007 um Saved Z = 42 at 28.46 min Tilt = 32.00 Mean = 81 Exp = 2.474 X/Y/Z = 0.066 -0.182 1.131 Errors in X/Y Prediction 0.011 0.039 um, 0.9 3.0 % Predicted X = 0.054 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.950 StdErr = 0.070 nFit = 5 nDrop = 0 Predicted Z = 4.256 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, 0.068 um; position 0.052, -1.018 Drift reached 3.6 A/sec after 7.7 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.22 nm/sec Tracking image shifted 0.001, 0.012 um; position 0.051, -1.033 Running script # 14 5.6 0.1 unbinned pixels; 0.6 1.9 nm along two shift axes; -0.2 2.0 nm on specimen axes Rate = 9.80958 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.12.22_tomo_02_043_-54.0.tif Record image shifted 0.000, 0.009 um Tracking reference shifted by 26.0,-2.5 pixels, 0.000,0.009 um Saved Z = 43 at 29.45 min Tilt = -54.00 Mean = 78 Exp = 3.473 X/Y/Z = 0.051 -1.044 4.257 Errors in X/Y Prediction -0.003 -0.094 um, 0.2 7.2 % Error in Z Prediction 0.001 Predicted X = 0.048 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -1.169 StdErr = 0.025 nFit = 5 nDrop = 0 Predicted Z = 4.295 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, -0.052 um; position 0.043, -1.118 Drift reached 2.4 A/sec after 7.9 sec, going on Running script # 14 3.9 -0.2 unbinned pixels; 0.5 1.3 nm along two shift axes; -0.0 1.4 nm on specimen axes Rate = 6.90921 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 31 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.12.59_tomo_02_044_-56.0.tif Record image shifted -0.001, 0.014 um Tracking reference shifted by 40.3,-1.8 pixels, -0.001,0.014 um Saved Z = 44 at 30.07 min Tilt = -56.00 Mean = 77 Exp = 3.775 X/Y/Z = 0.044 -1.135 4.295 Errors in X/Y Prediction -0.004 0.034 um, 0.3 2.6 % Predicted X = 0.037 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = -1.266 StdErr = 0.025 nFit = 6 nDrop = 0 Predicted Z = 4.338 StdErr = 0.024 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.066 um; position 0.038, -1.200 Drift reached 1.4 A/sec after 8.5 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.09 nm/sec Tracking image shifted 0.000, 0.004 um; position 0.039, -1.206 Running script # 14 1.8 -0.1 unbinned pixels; 0.3 0.6 nm along two shift axes; -0.0 0.6 nm on specimen axes Rate = 3.22138 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 35 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.13.56_tomo_02_045_-58.0.tif Record image shifted 0.001, 0.008 um Tracking reference shifted by 22.9,-4.7 pixels, 0.001,0.008 um Saved Z = 45 at 31.03 min Tilt = -58.00 Mean = 77 Exp = 4.146 X/Y/Z = 0.038 -1.215 4.307 Errors in X/Y Prediction 0.001 0.051 um, 0.1 3.9 % Error in Z Prediction -0.031 Predicted X = 0.028 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Y = -1.296 StdErr = 0.021 nFit = 5 nDrop = 1 Predicted Z = 4.355 StdErr = 0.022 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.012, 0.026 um; position 0.040, -1.322 Drift reached 1.3 A/sec after 8.9 sec, going on Running script # 14 2.4 0.4 unbinned pixels; 0.2 0.9 nm along two shift axes; -0.2 0.8 nm on specimen axes Rate = 4.3319 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 38 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.14.37_tomo_02_046_-60.0.tif Record image shifted -0.000, 0.016 um Tracking reference shifted by 45.0,-3.5 pixels, -0.000,0.016 um Saved Z = 46 at 31.70 min Tilt = -60.00 Mean = 76 Exp = 4.588 X/Y/Z = 0.041 -1.340 4.355 Errors in X/Y Prediction 0.013 -0.044 um, 1.0 3.4 % Predicted X = 0.047 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = -1.433 StdErr = 0.027 nFit = 6 nDrop = 0 Predicted Z = 4.393 StdErr = 0.024 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, 0.014 um; position 0.033, -1.448 Drift reached 0.7 A/sec after 9.7 sec, going on Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.07 nm/sec Tracking image shifted 0.000, 0.005 um; position 0.033, -1.454 Running script # 14 1.4 -0.1 unbinned pixels; 0.2 0.4 nm along two shift axes; 0.0 0.5 nm on specimen axes Rate = 2.4508 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 43 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.15.45_tomo_02_047_-62.0.tif Record image shifted 0.000, 0.010 um Tracking reference shifted by 28.7,-3.6 pixels, 0.000,0.010 um Saved Z = 47 at 32.85 min Tilt = -62.00 Mean = 75 Exp = 5.152 X/Y/Z = 0.033 -1.464 4.299 Errors in X/Y Prediction -0.014 -0.031 um, 1.1 2.4 % Error in Z Prediction -0.093 Predicted X = 0.062 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.252 StdErr = 0.067 nFit = 5 nDrop = 0 Predicted Z = 1.029 StdErr = 0.058 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.115 um; position 0.064, -0.137 Drift reached 9.5 A/sec after 6.5 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.51 nm/sec Tracking image shifted -0.009, -0.021 um; position 0.076, -0.110 Running script # 14 -6.8 5.7 unbinned pixels; 2.7 1.6 nm along two shift axes; -1.8 -2.6 nm on specimen axes Rate = 15.59392 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.16.43_tomo_02_048_34.0.tif Record image shifted -0.003, -0.021 um Tracking reference shifted by -57.9,13.4 pixels, -0.003,-0.021 um Saved Z = 48 at 33.78 min Tilt = 34.00 Mean = 81 Exp = 2.593 X/Y/Z = 0.080 -0.087 1.031 Errors in X/Y Prediction 0.018 0.165 um, 1.4 12.6 % Error in Z Prediction 0.002 Predicted X = 0.089 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = 0.012 StdErr = 0.059 nFit = 5 nDrop = 0 Predicted Z = 0.945 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.019, 0.195 um; position 0.070, -0.183 Drift reached 2.0 A/sec after 6.6 sec, going on Running script # 14 3.3 1.5 unbinned pixels; 0.1 1.3 nm along two shift axes; -0.6 1.1 nm on specimen axes Rate = 6.44726 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.17.19_tomo_02_049_36.0.tif Record image shifted 0.005, -0.004 um Tracking reference shifted by -13.8,-12.4 pixels, 0.005,-0.004 um Saved Z = 49 at 34.38 min Tilt = 36.00 Mean = 80 Exp = 2.713 X/Y/Z = 0.067 -0.180 0.945 Errors in X/Y Prediction -0.022 -0.192 um, 1.7 14.7 % Predicted X = 0.072 StdErr = 0.021 nFit = 3 nDrop = 0 Predicted Y = -0.154 StdErr = 0.136 nFit = 5 nDrop = 0 Predicted Z = 0.851 StdErr = 0.037 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.017, 0.115 um; position 0.055, -0.269 Drift reached 2.7 A/sec after 6.9 sec, going on Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.17 nm/sec Tracking image shifted -0.001, -0.007 um; position 0.058, -0.261 Running script # 14 -3.3 2.3 unbinned pixels; 1.1 0.8 nm along two shift axes; -0.7 -1.2 nm on specimen axes Rate = 7.04453 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.18.10_tomo_02_050_38.0.tif Record image shifted -0.005, -0.022 um Tracking reference shifted by -61.5,19.5 pixels, -0.005,-0.022 um Saved Z = 50 at 35.23 min Tilt = 38.00 Mean = 80 Exp = 2.872 X/Y/Z = 0.063 -0.238 0.890 Errors in X/Y Prediction -0.009 -0.084 um, 0.7 6.4 % Error in Z Prediction 0.040 Predicted X = 0.053 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.332 StdErr = 0.122 nFit = 5 nDrop = 0 Predicted Z = 0.790 StdErr = 0.047 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.009, -0.032 um; position 0.062, -0.301 Drift reached 2.1 A/sec after 7.1 sec, going on Running script # 14 2.0 0.8 unbinned pixels; 0.1 0.8 nm along two shift axes; -0.4 0.7 nm on specimen axes Rate = 3.76935 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.18.46_tomo_02_051_40.0.tif Record image shifted -0.001, -0.009 um Tracking reference shifted by -24.0,4.5 pixels, -0.001,-0.009 um Saved Z = 51 at 35.84 min Tilt = 40.00 Mean = 79 Exp = 3.033 X/Y/Z = 0.064 -0.294 0.790 Errors in X/Y Prediction 0.011 0.038 um, 0.8 2.9 % Predicted X = 0.073 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = -0.442 StdErr = 0.113 nFit = 5 nDrop = 0 Predicted Z = 0.701 StdErr = 0.054 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.012, -0.047 um; position 0.061, -0.394 Drift reached 1.6 A/sec after 7.3 sec, going on Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.12 nm/sec Tracking image shifted -0.004, -0.005 um; position 0.066, -0.389 Running script # 14 -2.1 2.5 unbinned pixels; 1.1 0.4 nm along two shift axes; -0.8 -0.8 nm on specimen axes Rate = 5.67865 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.19.40_tomo_02_052_42.0.tif Record image shifted 0.000, -0.021 um Tracking reference shifted by -60.7,4.9 pixels, 0.000,-0.021 um Saved Z = 52 at 36.73 min Tilt = 42.00 Mean = 79 Exp = 3.264 X/Y/Z = 0.067 -0.367 0.733 Errors in X/Y Prediction -0.006 0.075 um, 0.5 5.8 % Error in Z Prediction 0.032 Predicted X = 0.029 StdErr = 0.014 nFit = 4 nDrop = 0 Predicted Y = -1.609 StdErr = 0.022 nFit = 5 nDrop = 1 Predicted Z = 4.351 StdErr = 0.055 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.021 um; position 0.031, -1.589 Drift reached 3.0 A/sec after 10.5 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.15 nm/sec Tracking image shifted -0.001, 0.014 um; position 0.032, -1.606 Running script # 14 3.1 0.1 unbinned pixels; 0.3 1.0 nm along two shift axes; -0.1 1.1 nm on specimen axes Rate = 5.445 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 49 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.20.52_tomo_02_053_-64.0.tif Record image shifted -0.000, 0.018 um Tracking reference shifted by 51.7,-3.5 pixels, -0.000,0.018 um Saved Z = 53 at 37.98 min Tilt = -64.00 Mean = 72 Exp = 5.866 X/Y/Z = 0.033 -1.625 4.284 Errors in X/Y Prediction 0.004 -0.016 um, 0.3 1.2 % Error in Z Prediction -0.067 Predicted X = 0.026 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Y = -1.793 StdErr = 0.017 nFit = 6 nDrop = 0 Predicted Z = 4.304 StdErr = 0.032 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.100 um; position 0.026, -1.693 Drift reached 1.3 A/sec after 11.7 sec, going on Measured defocus = -3.89 microns changed by -0.11 to target drift = 0.04 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.03 nm/sec Tracking image shifted -0.003, 0.001 um; position 0.031, -1.695 Running script # 14 0.6 0.4 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.26436 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 58 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.22.24_tomo_02_054_-66.0.tif Record image shifted -0.000, 0.016 um Tracking reference shifted by 44.8,-2.6 pixels, -0.000,0.016 um Saved Z = 54 at 39.53 min Tilt = -66.00 Mean = 72 Exp = 6.918 X/Y/Z = 0.032 -1.711 4.189 Errors in X/Y Prediction 0.006 0.082 um, 0.5 6.3 % Error in Z Prediction -0.115 Predicted X = 0.037 StdErr = 0.005 nFit = 4 nDrop = 0 Predicted Y = -1.867 StdErr = 0.049 nFit = 6 nDrop = 0 Predicted Z = 4.240 StdErr = 0.068 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.115 um; position 0.032, -1.752 Drift reached 0.5 A/sec after 13.3 sec, going on Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.02 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.01 nm/sec Tracking image shifted -0.002, 0.005 um; position 0.034, -1.757 Running script # 14 0.6 0.1 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 1.03915 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 68 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.24.06_tomo_02_055_-68.0.tif Record image shifted -0.000, 0.017 um Tracking reference shifted by 47.6,-3.7 pixels, -0.000,0.017 um Saved Z = 55 at 41.25 min Tilt = -68.00 Mean = 69 Exp = 8.199 X/Y/Z = 0.035 -1.774 4.106 Errors in X/Y Prediction -0.002 0.093 um, 0.2 7.1 % Error in Z Prediction -0.133 Predicted X = 0.068 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.414 StdErr = 0.028 nFit = 5 nDrop = 0 Predicted Z = 0.661 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.021, -0.007 um; position 0.090, -0.407 Drift reached 2.8 A/sec after 7.7 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.08 nm/sec Tracking image shifted -0.010, -0.008 um; position 0.102, -0.399 Running script # 14 -2.5 3.1 unbinned pixels; 1.3 0.5 nm along two shift axes; -1.0 -1.0 nm on specimen axes Rate = 6.96589 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.25.12_tomo_02_056_44.0.tif Record image shifted -0.000, -0.017 um Tracking reference shifted by -46.8,5.4 pixels, -0.000,-0.017 um Saved Z = 56 at 42.28 min Tilt = 44.00 Mean = 79 Exp = 3.502 X/Y/Z = 0.103 -0.381 0.633 Errors in X/Y Prediction 0.035 0.033 um, 2.7 2.5 % Error in Z Prediction -0.028 Predicted X = 0.117 StdErr = 0.025 nFit = 3 nDrop = 0 Predicted Y = -0.411 StdErr = 0.026 nFit = 6 nDrop = 0 Predicted Z = 0.566 StdErr = 0.019 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.035, 0.091 um; position 0.082, -0.502 Drift reached 1.5 A/sec after 7.9 sec, going on Measured defocus = -4.14 microns changed by 0.14 to target drift = 0.08 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.07 nm/sec Tracking image shifted 0.001, -0.001 um; position 0.082, -0.501 Running script # 14 -1.2 0.8 unbinned pixels; 0.4 0.3 nm along two shift axes; -0.2 -0.4 nm on specimen axes Rate = 2.46563 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 31 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.26.21_tomo_02_057_46.0.tif Record image shifted -0.000, -0.018 um Tracking reference shifted by -50.4,5.2 pixels, -0.000,-0.018 um Saved Z = 57 at 43.43 min Tilt = 46.00 Mean = 77 Exp = 3.759 X/Y/Z = 0.083 -0.483 0.708 Errors in X/Y Prediction -0.034 -0.071 um, 2.6 5.5 % Error in Z Prediction 0.142 Predicted X = 0.100 StdErr = 0.042 nFit = 3 nDrop = 0 Predicted Y = -0.541 StdErr = 0.057 nFit = 5 nDrop = 0 Predicted Z = 0.573 StdErr = 0.079 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.020, 0.013 um; position 0.081, -0.555 Drift reached 3.0 A/sec after 8.5 sec, going on Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.13 nm/sec Tracking image shifted -0.005, -0.007 um; position 0.088, -0.545 Running script # 14 -2.2 1.1 unbinned pixels; 0.6 0.6 nm along two shift axes; -0.3 -0.8 nm on specimen axes Rate = 4.34277 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 34 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.27.20_tomo_02_058_48.0.tif Record image shifted -0.000, -0.019 um Tracking reference shifted by -53.7,5.9 pixels, -0.000,-0.019 um Saved Z = 58 at 44.42 min Tilt = 48.00 Mean = 77 Exp = 4.124 X/Y/Z = 0.088 -0.526 0.625 2021/01/12 12:28 D:\Users\Ben\210112_Titan2_Jans_cells\SEM\tomo_02.st 0 59 3838 3710 1680 -10.0 48.0 2.00 REG 44.4 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.001 um; position 0.000, -0.001 Drift reached 0.5 A/sec after 5.5 sec, going on Measured defocus = -6.33 microns changed by 2.33 to target drift = 0.03 nm/sec Tracking image shifted -0.001, 0.002 um; position 0.001, -0.003 Running script # 14 0.2 -0.3 unbinned pixels; 0.1 0.0 nm along two shift axes; 0.1 0.1 nm on specimen axes Rate = 0.68779 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.30.50_tomo_03_000_-10.0.tif Record image shifted 0.002, 0.003 um Tracking reference shifted by 7.4,-7.3 pixels, 0.002,0.003 um Saved Z = 0 at 0.00 min Tilt = -10.00 Mean = 94 Exp = 1.680 X/Y/Z = -0.001 -0.006 2.958 Need regular tracking - Need autofocus Tracking image shifted -0.002, 0.052 um; position 0.001, -0.057 Drift reached 0.3 A/sec after 5.5 sec, going on Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.05 nm/sec Tracking image shifted 0.001, -0.004 um; position 0.001, -0.053 Running script # 14 -1.1 -0.2 unbinned pixels; 0.1 0.4 nm along two shift axes; 0.1 -0.4 nm on specimen axes Rate = 1.88679 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.31.36_tomo_03_001_-12.0.tif Record image shifted -0.000, 0.001 um Tracking reference shifted by 3.0,-0.0 pixels, -0.000,0.001 um Saved Z = 1 at 0.85 min Tilt = -12.00 Mean = 89 Exp = 1.614 X/Y/Z = 0.001 -0.054 3.013 Predicted X = 0.002 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.101 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.069 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, -0.035 um; position 0.003, -0.066 Drift reached 4.2 A/sec after 5.5 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.19 nm/sec Tracking image shifted 0.000, 0.008 um; position 0.002, -0.077 Running script # 14 2.0 -0.4 unbinned pixels; 0.4 0.6 nm along two shift axes; 0.1 0.7 nm on specimen axes Rate = 3.63991 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.32.24_tomo_03_002_-14.0.tif Record image shifted 0.001, 0.007 um Tracking reference shifted by 19.9,-4.9 pixels, 0.001,0.007 um Saved Z = 2 at 1.57 min Tilt = -14.00 Mean = 90 Exp = 1.623 X/Y/Z = 0.001 -0.084 3.071 Errors in X/Y Prediction -0.001 0.017 um, 0.1 1.3 % Error in Z Prediction 0.003 Need regular tracking - Need autofocus Tracking image shifted -0.012, 0.035 um; position 0.011, -0.041 Drift reached 0.5 A/sec after 5.6 sec, going on Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.06 nm/sec Tracking image shifted 0.001, -0.005 um; position 0.010, -0.036 Running script # 14 -1.1 -0.1 unbinned pixels; 0.1 0.4 nm along two shift axes; 0.1 -0.4 nm on specimen axes Rate = 1.89226 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.33.08_tomo_03_003_-8.0.tif Record image shifted 0.002, -0.004 um Tracking reference shifted by -12.3,-3.2 pixels, 0.002,-0.004 um Saved Z = 3 at 2.31 min Tilt = -8.00 Mean = 90 Exp = 1.614 X/Y/Z = 0.009 -0.031 2.836 Need regular tracking - Need autofocus Tracking image shifted -0.002, 0.007 um; position 0.010, -0.038 Drift reached 3.0 A/sec after 5.7 sec, going on Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.11 nm/sec Tracking image shifted 0.000, 0.007 um; position 0.010, -0.047 Running script # 14 0.8 -0.3 unbinned pixels; 0.2 0.2 nm along two shift axes; 0.1 0.3 nm on specimen axes Rate = 1.4975 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.33.51_tomo_03_004_-6.0.tif Record image shifted 0.001, 0.001 um Tracking reference shifted by 3.7,-2.9 pixels, 0.001,0.001 um Saved Z = 4 at 3.03 min Tilt = -6.00 Mean = 90 Exp = 1.610 X/Y/Z = 0.009 -0.048 2.747 Predicted X = 0.003 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.127 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Z = 3.128 StdErr = 0.002 nFit = 3 nDrop = 0 Drift reached 0.4 A/sec after 5.1 sec, going on Running script # 14 -1.6 0.0 unbinned pixels; 0.2 0.5 nm along two shift axes; 0.0 -0.6 nm on specimen axes Rate = 2.78392 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.34.21_tomo_03_005_-16.0.tif Record image shifted -0.001, -0.001 um Saved Z = 5 at 3.53 min Tilt = -16.00 Mean = 89 Exp = 1.630 X/Y/Z = 0.004 -0.125 3.128 Errors in X/Y Prediction 0.001 0.002 um, 0.1 0.1 % Predicted X = 0.005 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.159 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Z = 3.185 StdErr = 0.003 nFit = 3 nDrop = 0 Need autofocus Drift reached 2.1 A/sec after 5.1 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.20 nm/sec Running script # 14 5.0 -1.0 unbinned pixels; 0.9 1.5 nm along two shift axes; 0.2 1.8 nm on specimen axes Rate = 8.84328 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.35.03_tomo_03_006_-18.0.tif Record image shifted 0.004, -0.020 um Saved Z = 6 at 4.23 min Tilt = -18.00 Mean = 89 Exp = 1.648 X/Y/Z = 0.001 -0.141 3.189 Errors in X/Y Prediction -0.004 0.018 um, 0.3 1.4 % Error in Z Prediction 0.005 Predicted X = 0.010 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.065 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 2.658 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.008 um; position 0.005, -0.058 Drift reached 1.2 A/sec after 5.5 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.09 nm/sec Tracking image shifted -0.000, -0.007 um; position 0.006, -0.050 Running script # 14 -3.1 -0.1 unbinned pixels; 0.3 1.0 nm along two shift axes; 0.1 -1.1 nm on specimen axes Rate = 5.48422 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.35.58_tomo_03_007_-4.0.tif Record image shifted 0.001, -0.001 um Tracking reference shifted by -4.2,-2.9 pixels, 0.001,-0.001 um Saved Z = 7 at 5.14 min Tilt = -4.00 Mean = 90 Exp = 1.610 X/Y/Z = 0.005 -0.048 2.671 Errors in X/Y Prediction -0.005 0.018 um, 0.4 1.3 % Error in Z Prediction 0.014 Predicted X = 0.003 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.059 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Z = 2.587 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.000, -0.020 um; position 0.003, -0.039 Drift reached 2.8 A/sec after 5.5 sec, going on Running script # 14 2.9 -0.1 unbinned pixels; 0.4 1.0 nm along two shift axes; -0.1 1.0 nm on specimen axes Rate = 5.10249 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.36.29_tomo_03_008_-2.0.tif Record image shifted 0.000, 0.010 um Tracking reference shifted by 28.2,-3.1 pixels, 0.000,0.010 um Saved Z = 8 at 5.66 min Tilt = -2.00 Mean = 90 Exp = 1.610 X/Y/Z = 0.003 -0.051 2.587 Errors in X/Y Prediction -0.000 0.008 um, 0.0 0.6 % Predicted X = 0.002 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.173 StdErr = 0.018 nFit = 3 nDrop = 0 Predicted Z = 3.248 StdErr = 0.001 nFit = 3 nDrop = 0 Drift reached 0.2 A/sec after 5.1 sec, going on Running script # 14 -1.0 0.1 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.0 -0.4 nm on specimen axes Rate = 1.74651 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.37.00_tomo_03_009_-20.0.tif Record image shifted 0.001, 0.035 um Saved Z = 9 at 6.17 min Tilt = -20.00 Mean = 90 Exp = 1.671 X/Y/Z = 0.000 -0.208 3.248 Errors in X/Y Prediction -0.001 -0.035 um, 0.1 2.7 % Predicted X = -0.002 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.269 StdErr = 0.050 nFit = 4 nDrop = 0 Predicted Z = 3.307 StdErr = 0.001 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.072 um; position -0.000, -0.197 Drift reached 3.9 A/sec after 5.5 sec, going on Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.16 nm/sec Tracking image shifted -0.000, 0.007 um; position 0.000, -0.206 Running script # 14 1.7 0.1 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.1 0.6 nm on specimen axes Rate = 3.05748 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.37.41_tomo_03_010_-22.0.tif Record image shifted 0.003, 0.027 um Tracking reference shifted by 75.8,-14.4 pixels, 0.003,0.027 um Saved Z = 10 at 6.85 min Tilt = -22.00 Mean = 89 Exp = 1.671 X/Y/Z = -0.003 -0.234 3.361 Errors in X/Y Prediction -0.001 0.035 um, 0.1 2.7 % Error in Z Prediction 0.054 Predicted X = -0.000 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.052 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Z = 2.504 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.022 um; position 0.000, -0.075 Drift reached 2.8 A/sec after 5.5 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.15 nm/sec Tracking image shifted -0.001, -0.007 um; position 0.001, -0.065 Running script # 14 -2.0 0.7 unbinned pixels; 0.5 0.6 nm along two shift axes; -0.2 -0.7 nm on specimen axes Rate = 3.75453 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.38.27_tomo_03_011_0.0.tif Record image shifted 0.002, -0.004 um Tracking reference shifted by -12.9,-4.7 pixels, 0.002,-0.004 um Saved Z = 11 at 7.71 min Tilt = 0.00 Mean = 89 Exp = 1.610 X/Y/Z = -0.001 -0.060 2.511 Errors in X/Y Prediction -0.000 -0.008 um, 0.0 0.6 % Error in Z Prediction 0.007 Predicted X = -0.003 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.066 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Z = 2.433 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, -0.001 um; position -0.001, -0.064 Drift reached 3.1 A/sec after 5.5 sec, going on Running script # 14 3.5 0.2 unbinned pixels; 0.4 1.2 nm along two shift axes; -0.2 1.2 nm on specimen axes Rate = 6.22842 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.39.04_tomo_03_012_2.0.tif Record image shifted 0.000, 0.014 um Tracking reference shifted by 38.2,-4.6 pixels, 0.000,0.014 um Saved Z = 12 at 8.23 min Tilt = 2.00 Mean = 90 Exp = 1.622 X/Y/Z = -0.001 -0.081 2.433 Errors in X/Y Prediction 0.002 -0.015 um, 0.1 1.2 % Predicted X = -0.004 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.284 StdErr = 0.035 nFit = 5 nDrop = 0 Predicted Z = 3.425 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.002 um; position -0.003, -0.286 Drift reached 0.1 A/sec after 5.5 sec, going on Running script # 14 -0.3 0.4 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.1 -0.1 nm on specimen axes Rate = 0.83982 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.39.35_tomo_03_013_-24.0.tif Record image shifted 0.001, 0.010 um Tracking reference shifted by 27.2,-4.7 pixels, 0.001,0.010 um Saved Z = 13 at 8.77 min Tilt = -24.00 Mean = 88 Exp = 1.693 X/Y/Z = -0.004 -0.295 3.425 Errors in X/Y Prediction 0.000 -0.012 um, 0.0 0.9 % Predicted X = -0.006 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.356 StdErr = 0.035 nFit = 5 nDrop = 0 Predicted Z = 3.497 StdErr = 0.040 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, -0.055 um; position -0.006, -0.301 Drift reached 3.4 A/sec after 5.5 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.15 nm/sec Tracking image shifted -0.001, 0.004 um; position -0.005, -0.306 Running script # 14 1.3 0.3 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.1 0.4 nm on specimen axes Rate = 2.29692 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.40.17_tomo_03_014_-26.0.tif Record image shifted 0.001, 0.016 um Tracking reference shifted by 43.8,-7.3 pixels, 0.001,0.016 um Saved Z = 14 at 9.46 min Tilt = -26.00 Mean = 88 Exp = 1.725 X/Y/Z = -0.006 -0.322 3.455 Errors in X/Y Prediction 0.000 0.033 um, 0.0 2.5 % Error in Z Prediction -0.042 Predicted X = -0.003 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = -0.109 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Z = 2.354 StdErr = 0.005 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.000 um; position -0.002, -0.108 Drift reached 3.4 A/sec after 5.5 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.19 nm/sec Tracking image shifted -0.002, -0.008 um; position -0.000, -0.099 Running script # 14 -2.7 1.2 unbinned pixels; 0.7 0.7 nm along two shift axes; -0.3 -1.0 nm on specimen axes Rate = 5.13424 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.41.03_tomo_03_015_4.0.tif Record image shifted 0.002, -0.002 um Tracking reference shifted by -5.9,-4.9 pixels, 0.002,-0.002 um Saved Z = 15 at 10.22 min Tilt = 4.00 Mean = 89 Exp = 1.628 X/Y/Z = -0.002 -0.096 2.377 Errors in X/Y Prediction 0.001 0.013 um, 0.1 1.0 % Error in Z Prediction 0.023 Predicted X = -0.000 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = -0.122 StdErr = 0.008 nFit = 5 nDrop = 0 Predicted Z = 2.299 StdErr = 0.017 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.009, -0.017 um; position -0.009, -0.105 Drift reached 3.0 A/sec after 5.5 sec, going on Running script # 14 3.5 0.3 unbinned pixels; 0.3 1.2 nm along two shift axes; -0.2 1.2 nm on specimen axes Rate = 6.11076 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.41.35_tomo_03_016_6.0.tif Record image shifted -0.000, 0.017 um Tracking reference shifted by 49.1,-4.2 pixels, -0.000,0.017 um Saved Z = 16 at 10.76 min Tilt = 6.00 Mean = 90 Exp = 1.641 X/Y/Z = -0.008 -0.126 2.299 Errors in X/Y Prediction -0.008 -0.003 um, 0.6 0.3 % Predicted X = -0.007 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.353 StdErr = 0.030 nFit = 5 nDrop = 0 Predicted Z = 3.534 StdErr = 0.050 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.004, -0.004 um; position -0.003, -0.350 Drift reached 1.4 A/sec after 5.5 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.09 nm/sec Tracking image shifted -0.001, 0.004 um; position -0.002, -0.354 Running script # 14 0.9 -0.2 unbinned pixels; 0.2 0.3 nm along two shift axes; 0.0 0.3 nm on specimen axes Rate = 1.52775 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.42.20_tomo_03_017_-28.0.tif Record image shifted 0.002, 0.015 um Tracking reference shifted by 42.1,-8.0 pixels, 0.002,0.015 um Saved Z = 17 at 11.51 min Tilt = -28.00 Mean = 88 Exp = 1.758 X/Y/Z = -0.004 -0.370 3.508 Errors in X/Y Prediction 0.003 -0.016 um, 0.3 1.2 % Error in Z Prediction -0.026 Predicted X = -0.005 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.414 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 3.581 StdErr = 0.036 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.034 um; position -0.010, -0.379 Drift reached 0.7 A/sec after 5.7 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.04 nm/sec Tracking image shifted -0.001, -0.001 um; position -0.008, -0.378 Running script # 14 -0.4 0.5 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 -0.2 nm on specimen axes Rate = 1.09071 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.43.03_tomo_03_018_-30.0.tif Record image shifted 0.001, 0.016 um Tracking reference shifted by 44.9,-5.9 pixels, 0.001,0.016 um Saved Z = 18 at 12.22 min Tilt = -30.00 Mean = 88 Exp = 1.788 X/Y/Z = -0.008 -0.394 3.551 Errors in X/Y Prediction -0.004 0.020 um, 0.3 1.5 % Error in Z Prediction -0.031 Predicted X = -0.016 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = -0.157 StdErr = 0.006 nFit = 6 nDrop = 0 Predicted Z = 2.226 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.011, -0.025 um; position -0.005, -0.132 Drift reached 5.3 A/sec after 5.5 sec, going on Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.39 nm/sec Tracking image shifted -0.003, -0.015 um; position -0.002, -0.114 Running script # 14 -4.6 5.1 unbinned pixels; 2.2 0.9 nm along two shift axes; -1.6 -1.8 nm on specimen axes Rate = 12.01819 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.43.50_tomo_03_019_8.0.tif Record image shifted 0.004, -0.002 um Tracking reference shifted by -6.9,-10.2 pixels, 0.004,-0.002 um Saved Z = 19 at 13.00 min Tilt = 8.00 Mean = 89 Exp = 1.647 X/Y/Z = -0.004 -0.110 2.284 Errors in X/Y Prediction 0.013 0.047 um, 1.0 3.6 % Error in Z Prediction 0.059 Predicted X = -0.001 StdErr = 0.011 nFit = 4 nDrop = 0 Predicted Y = -0.123 StdErr = 0.024 nFit = 6 nDrop = 0 Predicted Z = 2.221 StdErr = 0.026 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, 0.013 um; position -0.007, -0.136 Drift reached 3.4 A/sec after 5.6 sec, going on Running script # 14 3.5 0.8 unbinned pixels; 0.1 1.3 nm along two shift axes; -0.4 1.2 nm on specimen axes Rate = 6.32792 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.44.21_tomo_03_020_10.0.tif Record image shifted -0.000, 0.023 um Tracking reference shifted by 63.9,-5.0 pixels, -0.000,0.023 um Saved Z = 20 at 13.52 min Tilt = 10.00 Mean = 89 Exp = 1.669 X/Y/Z = -0.006 -0.161 2.221 Errors in X/Y Prediction -0.006 -0.038 um, 0.5 2.9 % Predicted X = -0.009 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -0.414 StdErr = 0.020 nFit = 5 nDrop = 0 Predicted Z = 3.600 StdErr = 0.005 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, -0.051 um; position -0.002, -0.364 Drift reached 2.7 A/sec after 5.7 sec, going on Running script # 14 2.6 0.9 unbinned pixels; 0.0 1.0 nm along two shift axes; -0.4 0.9 nm on specimen axes Rate = 4.90383 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.44.54_tomo_03_021_-32.0.tif Record image shifted 0.002, 0.022 um Tracking reference shifted by 61.6,-10.6 pixels, 0.002,0.022 um Saved Z = 21 at 14.09 min Tilt = -32.00 Mean = 88 Exp = 1.821 X/Y/Z = -0.003 -0.388 3.600 Errors in X/Y Prediction 0.006 0.026 um, 0.4 2.0 % Predicted X = -0.004 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.386 StdErr = 0.028 nFit = 5 nDrop = 0 Predicted Z = 3.648 StdErr = 0.006 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.029 um; position -0.007, -0.357 Drift reached 0.5 A/sec after 5.7 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.04 nm/sec Tracking image shifted -0.002, -0.002 um; position -0.005, -0.355 Running script # 14 -0.4 0.6 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 -0.1 nm on specimen axes Rate = 1.25745 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.45.37_tomo_03_022_-34.0.tif Record image shifted 0.001, 0.019 um Tracking reference shifted by 53.6,-7.7 pixels, 0.001,0.019 um Saved Z = 22 at 14.80 min Tilt = -34.00 Mean = 88 Exp = 1.861 X/Y/Z = -0.006 -0.374 3.633 Errors in X/Y Prediction -0.002 0.012 um, 0.2 0.9 % Error in Z Prediction -0.015 Predicted X = -0.004 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.173 StdErr = 0.032 nFit = 6 nDrop = 0 Predicted Z = 2.164 StdErr = 0.031 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.014 um; position -0.006, -0.187 Drift reached 7.5 A/sec after 5.5 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.47 nm/sec Tracking image shifted -0.005, -0.031 um; position 0.000, -0.151 Running script # 14 -7.2 2.0 unbinned pixels; 1.5 2.2 nm along two shift axes; -0.5 -2.6 nm on specimen axes Rate = 13.16836 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.46.35_tomo_03_023_12.0.tif Record image shifted 0.002, -0.002 um Tracking reference shifted by -6.3,-4.2 pixels, 0.002,-0.002 um Saved Z = 23 at 15.75 min Tilt = 12.00 Mean = 88 Exp = 1.681 X/Y/Z = -0.001 -0.146 2.158 Errors in X/Y Prediction 0.003 0.027 um, 0.2 2.0 % Error in Z Prediction -0.006 Predicted X = -0.001 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.160 StdErr = 0.036 nFit = 6 nDrop = 0 Predicted Z = 2.103 StdErr = 0.017 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.009, 0.039 um; position -0.010, -0.199 Drift reached 4.5 A/sec after 5.5 sec, going on Running script # 14 6.6 2.4 unbinned pixels; 0.0 2.5 nm along two shift axes; -1.1 2.2 nm on specimen axes Rate = 12.29756 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.47.06_tomo_03_024_14.0.tif Record image shifted 0.001, 0.028 um Tracking reference shifted by 80.0,-11.2 pixels, 0.001,0.028 um Saved Z = 24 at 16.28 min Tilt = 14.00 Mean = 89 Exp = 1.711 X/Y/Z = -0.010 -0.232 2.103 Errors in X/Y Prediction -0.008 -0.072 um, 0.7 5.5 % Predicted X = -0.004 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.330 StdErr = 0.010 nFit = 5 nDrop = 0 Predicted Z = 3.680 StdErr = 0.009 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, 0.019 um; position 0.001, -0.349 Drift reached 2.5 A/sec after 5.7 sec, going on Running script # 14 3.6 0.1 unbinned pixels; 0.4 1.2 nm along two shift axes; -0.1 1.3 nm on specimen axes Rate = 6.40433 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.47.40_tomo_03_025_-36.0.tif Record image shifted 0.001, 0.028 um Tracking reference shifted by 78.3,-11.0 pixels, 0.001,0.028 um Saved Z = 25 at 16.85 min Tilt = -36.00 Mean = 87 Exp = 1.909 X/Y/Z = -0.000 -0.380 3.680 Errors in X/Y Prediction 0.003 -0.050 um, 0.2 3.8 % Predicted X = -0.001 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.359 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 3.724 StdErr = 0.010 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.021 um; position -0.006, -0.379 Drift reached 0.4 A/sec after 5.9 sec, going on Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.04 nm/sec Tracking image shifted -0.001, -0.000 um; position -0.005, -0.379 Running script # 14 -0.1 0.7 unbinned pixels; 0.3 0.1 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.2854 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.48.24_tomo_03_026_-38.0.tif Record image shifted 0.002, 0.021 um Tracking reference shifted by 58.7,-9.4 pixels, 0.002,0.021 um Saved Z = 26 at 17.58 min Tilt = -38.00 Mean = 87 Exp = 1.967 X/Y/Z = -0.006 -0.400 3.763 Errors in X/Y Prediction -0.005 -0.041 um, 0.4 3.1 % Error in Z Prediction 0.040 Predicted X = -0.015 StdErr = 0.015 nFit = 4 nDrop = 0 Predicted Y = -0.272 StdErr = 0.052 nFit = 6 nDrop = 0 Predicted Z = 2.045 StdErr = 0.019 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, -0.075 um; position -0.008, -0.197 Drift reached 12.5 A/sec after 5.6 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.49 nm/sec Tracking image shifted -0.006, -0.029 um; position -0.000, -0.161 Running script # 14 -1.0 3.3 unbinned pixels; 1.2 0.1 nm along two shift axes; -1.1 -0.5 nm on specimen axes Rate = 6.09884 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.49.12_tomo_03_027_16.0.tif Record image shifted 0.002, 0.005 um Tracking reference shifted by 12.8,-7.9 pixels, 0.002,0.005 um Saved Z = 27 at 18.38 min Tilt = 16.00 Mean = 88 Exp = 1.726 X/Y/Z = -0.001 -0.165 1.999 Errors in X/Y Prediction 0.014 0.107 um, 1.1 8.2 % Error in Z Prediction -0.046 Predicted X = -0.004 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -0.149 StdErr = 0.096 nFit = 5 nDrop = 0 Predicted Z = 1.953 StdErr = 0.033 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, 0.081 um; position -0.010, -0.230 Drift reached 9.0 A/sec after 5.7 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.36 nm/sec Tracking image shifted -0.004, 0.014 um; position -0.003, -0.249 Running script # 14 2.7 1.7 unbinned pixels; 0.3 1.1 nm along two shift axes; -0.7 0.9 nm on specimen axes Rate = 5.67996 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.50.02_tomo_03_028_18.0.tif Record image shifted 0.000, 0.024 um Tracking reference shifted by 68.5,-6.6 pixels, 0.000,0.024 um Saved Z = 28 at 19.20 min Tilt = 18.00 Mean = 89 Exp = 1.768 X/Y/Z = -0.002 -0.274 1.899 Errors in X/Y Prediction 0.002 -0.125 um, 0.2 9.6 % Error in Z Prediction -0.054 Predicted X = -0.008 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Y = -0.424 StdErr = 0.013 nFit = 5 nDrop = 1 Predicted Z = 3.802 StdErr = 0.024 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.009, -0.026 um; position 0.001, -0.398 Drift reached 3.7 A/sec after 5.9 sec, going on Running script # 14 3.7 0.2 unbinned pixels; 0.4 1.2 nm along two shift axes; -0.2 1.3 nm on specimen axes Rate = 6.44699 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.50.37_tomo_03_029_-40.0.tif Record image shifted 0.002, 0.036 um Tracking reference shifted by 100.2,-13.4 pixels, 0.002,0.036 um Saved Z = 29 at 19.80 min Tilt = -40.00 Mean = 87 Exp = 2.033 X/Y/Z = 0.000 -0.437 3.802 Errors in X/Y Prediction 0.008 -0.013 um, 0.6 1.0 % Predicted X = -0.002 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.491 StdErr = 0.002 nFit = 5 nDrop = 0 Predicted Z = 3.853 StdErr = 0.027 nFit = 4 nDrop = 0 Need autofocus Drift reached 0.3 A/sec after 5.7 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.05 nm/sec Running script # 14 0.4 0.8 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.65777 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.51.17_tomo_03_030_-42.0.tif Record image shifted 0.004, -0.038 um Saved Z = 30 at 20.47 min Tilt = -42.00 Mean = 85 Exp = 2.100 X/Y/Z = -0.005 -0.453 3.857 Errors in X/Y Prediction -0.003 0.038 um, 0.3 2.9 % Error in Z Prediction 0.004 Predicted X = -0.003 StdErr = 0.008 nFit = 3 nDrop = 0 Predicted Y = -0.492 StdErr = 0.021 nFit = 5 nDrop = 0 Predicted Z = 3.919 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, -0.085 um; position -0.004, -0.406 Drift reached 2.5 A/sec after 6.1 sec, going on Running script # 14 2.5 0.8 unbinned pixels; 0.0 0.9 nm along two shift axes; -0.3 0.9 nm on specimen axes Rate = 4.66236 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.51.48_tomo_03_031_-44.0.tif Record image shifted 0.001, 0.056 um Tracking reference shifted by 159.6,-17.4 pixels, 0.001,0.056 um Saved Z = 31 at 20.98 min Tilt = -44.00 Mean = 89 Exp = 2.229 X/Y/Z = -0.004 -0.465 3.919 Errors in X/Y Prediction -0.002 0.026 um, 0.1 2.0 % Predicted X = 0.007 StdErr = 0.017 nFit = 4 nDrop = 0 Predicted Y = -0.286 StdErr = 0.092 nFit = 6 nDrop = 0 Predicted Z = 1.837 StdErr = 0.025 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.005, -0.035 um; position 0.002, -0.251 Drift reached 4.0 A/sec after 5.7 sec, going on Running script # 14 -4.6 3.2 unbinned pixels; 1.6 1.1 nm along two shift axes; -1.0 -1.7 nm on specimen axes Rate = 9.76517 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.52.30_tomo_03_032_20.0.tif Record image shifted -0.004, -0.016 um Tracking reference shifted by -43.9,16.6 pixels, -0.004,-0.016 um Saved Z = 32 at 21.68 min Tilt = 20.00 Mean = 88 Exp = 1.797 X/Y/Z = 0.008 -0.232 1.837 Errors in X/Y Prediction 0.001 0.055 um, 0.1 4.2 % Predicted X = 0.011 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.234 StdErr = 0.132 nFit = 5 nDrop = 0 Predicted Z = 1.760 StdErr = 0.029 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.010, 0.082 um; position 0.001, -0.315 Drift reached 8.9 A/sec after 5.7 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.24 nm/sec Tracking image shifted -0.006, 0.014 um; position 0.010, -0.334 Running script # 14 -0.1 1.9 unbinned pixels; 0.7 0.2 nm along two shift axes; -0.7 -0.1 nm on specimen axes Rate = 3.40743 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.53.15_tomo_03_033_22.0.tif Record image shifted 0.009, 0.052 um Tracking reference shifted by 145.6,-38.2 pixels, 0.009,0.052 um Saved Z = 33 at 22.42 min Tilt = 22.00 Mean = 89 Exp = 1.849 X/Y/Z = 0.002 -0.386 1.729 Errors in X/Y Prediction -0.010 -0.152 um, 0.8 11.7 % Error in Z Prediction -0.032 Predicted X = 0.006 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.443 StdErr = 0.120 nFit = 6 nDrop = 0 Predicted Z = 1.644 StdErr = 0.012 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.022, -0.042 um; position -0.016, -0.401 Drift reached 8.1 A/sec after 5.7 sec, going on Running script # 14 -9.1 5.6 unbinned pixels; 2.9 2.3 nm along two shift axes; -1.7 -3.4 nm on specimen axes Rate = 18.74813 A/sec -7.0 3.3 unbinned pixels; 1.9 1.9 nm along two shift axes; -0.9 -2.5 nm on specimen axes Rate = 13.48216 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.53.52_tomo_03_034_24.0.tif Record image shifted -0.004, 0.005 um Tracking reference shifted by 14.8,9.5 pixels, -0.004,0.005 um Saved Z = 34 at 23.05 min Tilt = 24.00 Mean = 87 Exp = 1.872 X/Y/Z = -0.007 -0.396 1.644 Errors in X/Y Prediction -0.013 0.048 um, 1.0 3.7 % Predicted X = -0.007 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.476 StdErr = 0.016 nFit = 5 nDrop = 0 Predicted Z = 3.975 StdErr = 0.027 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, 0.013 um; position -0.002, -0.489 Drift reached 2.3 A/sec after 6.1 sec, going on Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.14 nm/sec Tracking image shifted -0.003, -0.005 um; position 0.000, -0.485 Running script # 14 1.6 0.5 unbinned pixels; 0.0 0.6 nm along two shift axes; -0.2 0.6 nm on specimen axes Rate = 3.02582 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.54.42_tomo_03_035_-46.0.tif Record image shifted 0.003, 0.042 um Tracking reference shifted by 118.0,-20.2 pixels, 0.003,0.042 um Saved Z = 35 at 23.89 min Tilt = -46.00 Mean = 85 Exp = 2.257 X/Y/Z = -0.003 -0.527 4.046 Errors in X/Y Prediction 0.005 -0.051 um, 0.4 3.9 % Error in Z Prediction 0.071 Predicted X = -0.002 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.564 StdErr = 0.038 nFit = 5 nDrop = 0 Predicted Z = 4.101 StdErr = 0.061 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, -0.026 um; position -0.008, -0.538 Drift reached 0.9 A/sec after 6.3 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.04 nm/sec Tracking image shifted -0.002, -0.000 um; position -0.006, -0.538 Running script # 14 0.2 0.9 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.3 0.0 nm on specimen axes Rate = 1.56951 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.55.28_tomo_03_036_-48.0.tif Record image shifted 0.001, 0.036 um Tracking reference shifted by 102.4,-12.8 pixels, 0.001,0.036 um Saved Z = 36 at 24.66 min Tilt = -48.00 Mean = 85 Exp = 2.377 X/Y/Z = -0.007 -0.574 4.083 Errors in X/Y Prediction -0.005 -0.010 um, 0.4 0.8 % Error in Z Prediction -0.019 Predicted X = -0.012 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = -0.631 StdErr = 0.027 nFit = 6 nDrop = 0 Predicted Z = 4.158 StdErr = 0.040 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, -0.062 um; position -0.007, -0.568 Drift reached 3.2 A/sec after 6.5 sec, going on Running script # 14 2.8 0.6 unbinned pixels; 0.1 1.0 nm along two shift axes; -0.3 1.0 nm on specimen axes Rate = 5.0315 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.56.01_tomo_03_037_-50.0.tif Record image shifted 0.001, 0.045 um Tracking reference shifted by 127.4,-13.4 pixels, 0.001,0.045 um Saved Z = 37 at 25.20 min Tilt = -50.00 Mean = 85 Exp = 2.510 X/Y/Z = -0.007 -0.617 4.158 Errors in X/Y Prediction 0.004 0.014 um, 0.3 1.1 % Predicted X = -0.011 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = -0.681 StdErr = 0.032 nFit = 5 nDrop = 0 Predicted Z = 4.226 StdErr = 0.045 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, -0.017 um; position -0.012, -0.663 Drift reached 0.4 A/sec after 6.7 sec, going on Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.03 nm/sec Tracking image shifted -0.001, 0.001 um; position -0.010, -0.665 Running script # 14 0.4 0.5 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.2 0.1 nm on specimen axes Rate = 1.1308 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.56.49_tomo_03_038_-52.0.tif Record image shifted 0.002, 0.042 um Tracking reference shifted by 119.5,-16.2 pixels, 0.002,0.042 um Saved Z = 38 at 26.00 min Tilt = -52.00 Mean = 84 Exp = 2.653 X/Y/Z = -0.012 -0.707 4.221 Errors in X/Y Prediction -0.001 -0.027 um, 0.1 2.0 % Error in Z Prediction -0.005 Predicted X = -0.015 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.461 StdErr = 0.138 nFit = 5 nDrop = 0 Predicted Z = 1.557 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.020, -0.076 um; position 0.005, -0.385 Drift reached 5.0 A/sec after 5.9 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.16 nm/sec Tracking image shifted -0.008, -0.011 um; position 0.016, -0.372 Running script # 14 -2.4 3.3 unbinned pixels; 1.4 0.4 nm along two shift axes; -1.1 -0.9 nm on specimen axes Rate = 7.20827 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.57.40_tomo_03_039_26.0.tif Record image shifted 0.011, 0.036 um Tracking reference shifted by 98.2,-39.8 pixels, 0.011,0.036 um Saved Z = 39 at 26.85 min Tilt = 26.00 Mean = 87 Exp = 1.936 X/Y/Z = 0.006 -0.406 1.523 Errors in X/Y Prediction 0.021 0.055 um, 1.6 4.2 % Error in Z Prediction -0.034 Predicted X = 0.005 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = -0.448 StdErr = 0.142 nFit = 5 nDrop = 0 Predicted Z = 1.435 StdErr = 0.015 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.011, 0.026 um; position -0.006, -0.474 Drift reached 3.5 A/sec after 5.9 sec, going on Running script # 14 -1.5 2.2 unbinned pixels; 0.9 0.2 nm along two shift axes; -0.7 -0.6 nm on specimen axes Rate = 4.67674 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.58.13_tomo_03_040_28.0.tif Record image shifted -0.003, 0.034 um Tracking reference shifted by 96.9,0.7 pixels, -0.003,0.034 um Saved Z = 40 at 27.40 min Tilt = 28.00 Mean = 87 Exp = 1.994 X/Y/Z = -0.001 -0.509 1.435 Errors in X/Y Prediction -0.006 -0.061 um, 0.4 4.7 % Predicted X = 0.004 StdErr = 0.028 nFit = 4 nDrop = 0 Predicted Y = -0.507 StdErr = 0.126 nFit = 5 nDrop = 0 Predicted Z = 1.341 StdErr = 0.017 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, 0.027 um; position -0.010, -0.533 Drift reached 5.3 A/sec after 5.9 sec, going on Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.23 nm/sec Tracking image shifted -0.006, -0.011 um; position -0.002, -0.519 Running script # 14 -3.4 1.5 unbinned pixels; 0.9 1.0 nm along two shift axes; -0.4 -1.2 nm on specimen axes Rate = 6.52941 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.58.59_tomo_03_041_30.0.tif Record image shifted 0.005, 0.017 um Tracking reference shifted by 46.4,-18.5 pixels, 0.005,0.017 um Saved Z = 41 at 28.16 min Tilt = 30.00 Mean = 86 Exp = 2.051 X/Y/Z = -0.007 -0.535 1.426 Errors in X/Y Prediction -0.011 -0.028 um, 0.8 2.2 % Error in Z Prediction 0.085 Predicted X = -0.014 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -0.632 StdErr = 0.066 nFit = 5 nDrop = 0 Predicted Z = 1.332 StdErr = 0.070 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.003, -0.058 um; position -0.011, -0.574 Drift reached 1.9 A/sec after 6.1 sec, going on Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.11 nm/sec Tracking image shifted -0.006, -0.003 um; position -0.004, -0.570 Running script # 14 -1.5 1.2 unbinned pixels; 0.6 0.4 nm along two shift axes; -0.4 -0.6 nm on specimen axes Rate = 3.36184 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_12.59.51_tomo_03_042_32.0.tif Record image shifted 0.001, 0.031 um Tracking reference shifted by 86.5,-10.5 pixels, 0.001,0.031 um Saved Z = 42 at 29.03 min Tilt = 32.00 Mean = 87 Exp = 2.136 X/Y/Z = -0.005 -0.600 1.369 Errors in X/Y Prediction 0.009 0.032 um, 0.7 2.4 % Error in Z Prediction 0.037 Predicted X = -0.014 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.776 StdErr = 0.034 nFit = 5 nDrop = 0 Predicted Z = 4.302 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.004, -0.009 um; position -0.010, -0.767 Drift reached 1.0 A/sec after 6.9 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.05 nm/sec Tracking image shifted -0.001, 0.004 um; position -0.008, -0.772 Running script # 14 0.9 0.4 unbinned pixels; 0.0 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.70711 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.00.45_tomo_03_043_-54.0.tif Record image shifted 0.005, 0.049 um Tracking reference shifted by 137.0,-25.5 pixels, 0.005,0.049 um Saved Z = 43 at 29.95 min Tilt = -54.00 Mean = 84 Exp = 2.829 X/Y/Z = -0.013 -0.821 4.275 Errors in X/Y Prediction 0.001 -0.044 um, 0.1 3.4 % Error in Z Prediction -0.027 Predicted X = -0.016 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.955 StdErr = 0.022 nFit = 5 nDrop = 0 Predicted Z = 4.335 StdErr = 0.018 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.011, -0.117 um; position -0.028, -0.839 Drift reached 1.0 A/sec after 7.1 sec, going on Running script # 14 0.5 1.1 unbinned pixels; 0.3 0.3 nm along two shift axes; -0.4 0.1 nm on specimen axes Rate = 2.11149 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.01.20_tomo_03_044_-56.0.tif Record image shifted -0.000, 0.051 um Tracking reference shifted by 143.0,-11.7 pixels, -0.000,0.051 um Saved Z = 44 at 30.53 min Tilt = -56.00 Mean = 83 Exp = 3.034 X/Y/Z = -0.026 -0.890 4.335 Errors in X/Y Prediction -0.010 0.066 um, 0.8 5.0 % Predicted X = -0.031 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -1.010 StdErr = 0.050 nFit = 5 nDrop = 0 Predicted Z = 4.395 StdErr = 0.021 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.008, -0.114 um; position -0.023, -0.896 Drift reached 0.9 A/sec after 7.3 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.06 nm/sec Tracking image shifted -0.002, -0.000 um; position -0.020, -0.896 Running script # 14 0.7 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.8085 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.02.12_tomo_03_045_-58.0.tif Record image shifted 0.003, 0.059 um Tracking reference shifted by 166.3,-23.1 pixels, 0.003,0.059 um Saved Z = 45 at 31.39 min Tilt = -58.00 Mean = 83 Exp = 3.274 X/Y/Z = -0.023 -0.956 4.417 Errors in X/Y Prediction 0.008 0.055 um, 0.6 4.2 % Error in Z Prediction 0.023 Predicted X = -0.031 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = -1.009 StdErr = 0.026 nFit = 5 nDrop = 1 Predicted Z = 4.482 StdErr = 0.010 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, -0.030 um; position -0.033, -0.979 Drift reached 0.9 A/sec after 7.7 sec, going on Running script # 14 0.7 1.0 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.4 0.2 nm on specimen axes Rate = 2.07696 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 30 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.02.48_tomo_03_046_-60.0.tif Record image shifted -0.000, 0.057 um Tracking reference shifted by 161.5,-14.1 pixels, -0.000,0.057 um Saved Z = 46 at 32.02 min Tilt = -60.00 Mean = 82 Exp = 3.542 X/Y/Z = -0.033 -1.037 4.482 Errors in X/Y Prediction -0.002 -0.028 um, 0.1 2.1 % Predicted X = -0.034 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -1.087 StdErr = 0.032 nFit = 5 nDrop = 0 Predicted Z = 4.548 StdErr = 0.011 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Trimming A by 20 25, reference by 20 20 Tracking image shifted -0.004, -0.040 um; position -0.029, -1.047 Drift reached 1.2 A/sec after 8.1 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.03 nm/sec Tracking image shifted -0.001, 0.003 um; position -0.028, -1.051 Running script # 14 0.4 0.7 unbinned pixels; 0.2 0.2 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.41156 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.03.44_tomo_03_047_-62.0.tif Record image shifted 0.003, 0.068 um Tracking reference shifted by 192.0,-26.4 pixels, 0.003,0.068 um Saved Z = 47 at 32.95 min Tilt = -62.00 Mean = 81 Exp = 3.901 X/Y/Z = -0.031 -1.119 4.488 Errors in X/Y Prediction 0.003 -0.032 um, 0.2 2.5 % Error in Z Prediction -0.060 Predicted X = -0.008 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Y = -0.688 StdErr = 0.049 nFit = 6 nDrop = 0 Predicted Z = 1.284 StdErr = 0.050 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.017, -0.113 um; position 0.009, -0.575 Drift reached 3.6 A/sec after 6.1 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.12 nm/sec Tracking image shifted -0.007, -0.003 um; position 0.018, -0.572 Running script # 14 -2.3 1.4 unbinned pixels; 0.7 0.6 nm along two shift axes; -0.4 -0.9 nm on specimen axes Rate = 4.74654 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.04.39_tomo_03_048_34.0.tif Record image shifted 0.008, 0.022 um Tracking reference shifted by 59.5,-29.3 pixels, 0.008,0.022 um Saved Z = 48 at 33.83 min Tilt = 34.00 Mean = 86 Exp = 2.218 X/Y/Z = 0.010 -0.593 1.222 Errors in X/Y Prediction 0.018 0.095 um, 1.4 7.3 % Error in Z Prediction -0.062 Predicted X = 0.016 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = -0.578 StdErr = 0.045 nFit = 5 nDrop = 0 Predicted Z = 1.176 StdErr = 0.051 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.022, 0.111 um; position -0.006, -0.689 Drift reached 2.5 A/sec after 6.3 sec, going on Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.13 nm/sec Tracking image shifted -0.001, -0.002 um; position -0.004, -0.686 Running script # 14 -1.2 1.3 unbinned pixels; 0.6 0.2 nm along two shift axes; -0.4 -0.4 nm on specimen axes Rate = 3.04219 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.05.27_tomo_03_049_36.0.tif Record image shifted 0.001, 0.034 um Tracking reference shifted by 95.5,-12.3 pixels, 0.001,0.034 um Saved Z = 49 at 34.72 min Tilt = 36.00 Mean = 86 Exp = 2.330 X/Y/Z = -0.005 -0.719 1.208 Errors in X/Y Prediction -0.021 -0.141 um, 1.6 10.8 % Error in Z Prediction 0.032 Predicted X = -0.000 StdErr = 0.022 nFit = 3 nDrop = 0 Predicted Y = -0.798 StdErr = 0.081 nFit = 5 nDrop = 0 Predicted Z = 1.132 StdErr = 0.051 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.014, -0.100 um; position -0.014, -0.698 Drift reached 3.9 A/sec after 6.3 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.22 nm/sec Tracking image shifted -0.006, -0.009 um; position -0.006, -0.688 Running script # 14 -3.7 2.1 unbinned pixels; 1.1 1.0 nm along two shift axes; -0.6 -1.4 nm on specimen axes Rate = 7.43471 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.06.21_tomo_03_050_38.0.tif Record image shifted 0.001, 0.034 um Tracking reference shifted by 96.3,-10.8 pixels, 0.001,0.034 um Saved Z = 50 at 35.53 min Tilt = 38.00 Mean = 85 Exp = 2.431 X/Y/Z = -0.006 -0.721 1.120 Errors in X/Y Prediction -0.006 0.078 um, 0.5 6.0 % Error in Z Prediction -0.012 Predicted X = -0.017 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -0.783 StdErr = 0.097 nFit = 5 nDrop = 0 Predicted Z = 1.037 StdErr = 0.046 nFit = 5 nDrop = 0 Need regular tracking - Tracking image shifted -0.008, -0.023 um; position -0.009, -0.761 Drift reached 2.1 A/sec after 6.6 sec, going on Running script # 14 -2.3 2.5 unbinned pixels; 1.1 0.5 nm along two shift axes; -0.8 -0.9 nm on specimen axes Rate = 5.94034 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.06.56_tomo_03_051_40.0.tif Record image shifted -0.002, 0.037 um Tracking reference shifted by 104.3,-2.7 pixels, -0.002,0.037 um Saved Z = 51 at 36.11 min Tilt = 40.00 Mean = 85 Exp = 2.563 X/Y/Z = -0.004 -0.795 1.037 Errors in X/Y Prediction 0.012 -0.012 um, 0.9 0.9 % Predicted X = -0.005 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.861 StdErr = 0.097 nFit = 5 nDrop = 0 Predicted Z = 0.960 StdErr = 0.054 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.003, -0.045 um; position -0.002, -0.816 Drift reached 2.6 A/sec after 6.7 sec, going on Measured defocus = -4.12 microns changed by 0.12 to target drift = 0.18 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.12 nm/sec Tracking image shifted -0.006, -0.011 um; position 0.005, -0.804 Running script # 14 -3.4 4.0 unbinned pixels; 1.7 0.7 nm along two shift axes; -1.3 -1.3 nm on specimen axes Rate = 9.26961 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.07.57_tomo_03_052_42.0.tif Record image shifted 0.006, 0.042 um Tracking reference shifted by 118.3,-27.0 pixels, 0.006,0.042 um Saved Z = 52 at 37.14 min Tilt = 42.00 Mean = 84 Exp = 2.718 X/Y/Z = 0.000 -0.845 1.060 Errors in X/Y Prediction 0.005 0.016 um, 0.4 1.2 % Error in Z Prediction 0.101 Predicted X = -0.035 StdErr = 0.016 nFit = 4 nDrop = 0 Predicted Y = -1.209 StdErr = 0.007 nFit = 5 nDrop = 0 Predicted Z = 4.558 StdErr = 0.032 nFit = 4 nDrop = 0 Need autofocus Drift reached 0.6 A/sec after 8.3 sec, going on Measured defocus = -3.84 microns changed by -0.16 to target drift = 0.13 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.09 nm/sec Running script # 14 1.9 0.8 unbinned pixels; 0.0 0.7 nm along two shift axes; -0.3 0.6 nm on specimen axes Rate = 3.54683 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 36 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.09.07_tomo_03_053_-64.0.tif Record image shifted -0.004, 0.002 um Saved Z = 53 at 38.33 min Tilt = -64.00 Mean = 75 Exp = 4.315 X/Y/Z = -0.031 -1.211 4.406 Errors in X/Y Prediction 0.004 -0.002 um, 0.3 0.2 % Error in Z Prediction -0.152 Predicted X = -0.024 StdErr = 0.009 nFit = 4 nDrop = 0 Predicted Y = -1.307 StdErr = 0.005 nFit = 6 nDrop = 0 Predicted Z = 4.497 StdErr = 0.098 nFit = 4 nDrop = 0 Need autofocus Drift reached 0.6 A/sec after 9.3 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.05 nm/sec Running script # 14 0.4 1.3 unbinned pixels; 0.4 0.3 nm along two shift axes; -0.5 0.1 nm on specimen axes Rate = 2.42312 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 43 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.10.02_tomo_03_054_-66.0.tif Record image shifted 0.008, -0.024 um Saved Z = 54 at 39.26 min Tilt = -66.00 Mean = 76 Exp = 5.154 X/Y/Z = -0.031 -1.283 4.439 Errors in X/Y Prediction -0.007 0.024 um, 0.5 1.8 % Error in Z Prediction -0.057 Predicted X = -0.033 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = -1.376 StdErr = 0.014 nFit = 6 nDrop = 0 Predicted Z = 4.493 StdErr = 0.082 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.010, -0.286 um; position -0.023, -1.090 Trimming A by 60 20 50 20, reference by 60 20 50 20 Drift reached 0.6 A/sec after 10.5 sec, going on Measured defocus = -4.60 microns changed by 0.60 to target drift = 0.06 nm/sec Redoing Autofocus because measured defocus is too far from prediction Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.04 nm/sec Tracking image shifted -0.005, 0.038 um; position -0.017, -1.128 Running script # 14 Trimming A by 20 20 30 20, reference by 20 20 30 20 Trimming A by 20 20 20 20, reference by 20 20 30 20 3.2 1.3 unbinned pixels; 0.1 1.2 nm along two shift axes; -0.6 1.1 nm on specimen axes Rate = 6.07345 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 51 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.11.27_tomo_03_055_-68.0.tif Errors in X/Y Prediction 0.002 0.093 um, 0.1 7.1 % Error in Z Prediction -0.054 Need regular tracking - Need autofocus Tracking image shifted -0.021, 0.035 um; position 0.021, -0.880 Drift reached 1.3 A/sec after 6.9 sec, going on Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.14 nm/sec Tracking image shifted -0.004, -0.004 um; position 0.026, -0.876 Running script # 14 -2.8 1.7 unbinned pixels; 0.9 0.7 nm along two shift axes; -0.5 -1.0 nm on specimen axes Rate = 5.7694 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.12.41_tomo_03_056_44.0.tif Record image shifted 0.008, 0.035 um Tracking reference shifted by 98.1,-31.4 pixels, 0.008,0.035 um Saved Z = 55 at 41.88 min Tilt = 44.00 Mean = 83 Exp = 2.904 X/Y/Z = 0.018 -0.911 0.979 Need regular tracking - Need autofocus Tracking image shifted 0.017, 0.077 um; position 0.002, -0.988 Drift reached 1.5 A/sec after 7.3 sec, going on Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.08 nm/sec Tracking image shifted -0.000, -0.003 um; position 0.003, -0.984 Running script # 14 -0.8 1.2 unbinned pixels; 0.5 0.1 nm along two shift axes; -0.4 -0.3 nm on specimen axes Rate = 2.4963 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.13.34_tomo_03_057_46.0.tif Record image shifted 0.001, 0.048 um Tracking reference shifted by 134.3,-14.8 pixels, 0.001,0.048 um Saved Z = 56 at 42.76 min Tilt = 46.00 Mean = 83 Exp = 3.134 X/Y/Z = 0.002 -1.032 1.022 Predicted X = -0.014 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -1.152 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 1.065 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.016, -0.115 um; position 0.002, -1.037 Drift reached 2.8 A/sec after 7.5 sec, going on Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.15 nm/sec Tracking image shifted -0.004, -0.007 um; position 0.008, -1.028 Running script # 14 -2.7 1.4 unbinned pixels; 0.8 0.7 nm along two shift axes; -0.4 -1.0 nm on specimen axes Rate = 5.39103 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.14.28_tomo_03_058_48.0.tif Record image shifted 0.001, 0.047 um Tracking reference shifted by 132.4,-14.5 pixels, 0.001,0.047 um Saved Z = 57 at 43.67 min Tilt = 48.00 Mean = 82 Exp = 3.388 X/Y/Z = 0.007 -1.074 0.978 2021/01/12 13:14 D:\Users\Ben\210112_Titan2_Jans_cells\SEM\tomo_03.st 0 58 3838 3710 1651 -10.0 48.0 2.00 REG 43.7 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = -0.000, 0.000 Trimming A by 23 170, reference by 21 170 Angle = 18.00, shift = 7.0, 7.2 pixels, 0.015, 0.018 um, IS = -0.015, -0.018 Trimming A by 38 165, reference by 38 165 Angle = 11.00, shift = -65.8, -2.6 pixels, 0.011, -0.150 um, IS = -0.025, 0.132 Trimming A by 38 165, reference by 33 165 Angle = 4.00, shift = -32.0, -1.7 pixels, 0.004, -0.073 um, IS = -0.030, 0.205 Trimming A by 20 27 175 20, reference by 22 23 175 20 Angle = -4.00, shift = 29.8, -11.6 pixels, -0.034, 0.065 um, IS = 0.004, 0.141 Trimming A by 28 170, reference by 33 170 Angle = -11.00, shift = 7.3, -10.0 pixels, -0.024, 0.014 um, IS = 0.029, 0.127 Trimming A by 23 170, reference by 28 170 Angle = -18.00, shift = 37.0, 8.9 pixels, 0.011, 0.086 um, IS = 0.018, 0.040 Trimming A by 26 170, reference by 27 170 Angle = -24.00, shift = 41.8, 11.5 pixels, 0.015, 0.098 um, IS = 0.003, -0.057 General solution delZ -0.02, delY 2.32, yZero 0.17 solution with assumed YZero 0.17: delZ -0.02, delY 2.39 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.02 microns and lateral displacement of -2.32 microns Measured defocus = -6.46 microns changed by 2.46 to target drift = 0.17 nm/sec Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.000 um; position -0.001, 0.000 Drift reached 1.0 A/sec after 5.5 sec, going on Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.11 nm/sec Tracking image shifted 0.004, -0.001 um; position -0.005, 0.002 Running script # 14 -0.9 -2.0 unbinned pixels; 0.6 0.5 nm along two shift axes; 0.7 -0.3 nm on specimen axes Rate = 3.93346 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.23.20_tomo_04_000_-10.0.tif Record image shifted 0.012, -0.017 um Tracking reference shifted by -49.7,-29.6 pixels, 0.012,-0.017 um Saved Z = 0 at -0.00 min Tilt = -10.00 Mean = 104 Exp = 1.440 X/Y/Z = -0.018 0.018 3.510 Need regular tracking - Need autofocus Tracking image shifted -0.011, -0.218 um; position -0.006, 0.236 Drift reached 0.3 A/sec after 5.4 sec, going on Measured defocus = -4.13 microns changed by 0.13 to target drift = 0.10 nm/sec Tracking image shifted 0.001, -0.001 um; position -0.008, 0.237 Running script # 14 -0.4 -2.1 unbinned pixels; 0.6 0.4 nm along two shift axes; 0.7 -0.1 nm on specimen axes Rate = 3.73041 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.24.00_tomo_04_001_-12.0.tif Record image shifted -0.001, 0.003 um Tracking reference shifted by 7.4,1.9 pixels, -0.001,0.003 um Saved Z = 1 at 0.67 min Tilt = -12.00 Mean = 100 Exp = 1.384 X/Y/Z = -0.007 0.235 3.637 Predicted X = 0.003 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.452 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.765 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.008, 0.190 um; position -0.005, 0.262 Drift reached 1.3 A/sec after 5.4 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.11 nm/sec Tracking image shifted 0.006, -0.003 um; position -0.011, 0.265 Running script # 14 -1.4 -1.7 unbinned pixels; 0.4 0.7 nm along two shift axes; 0.6 -0.4 nm on specimen axes Rate = 3.80189 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.24.40_tomo_04_002_-14.0.tif Record image shifted -0.001, 0.003 um Tracking reference shifted by 7.5,1.4 pixels, -0.001,0.003 um Saved Z = 2 at 1.35 min Tilt = -14.00 Mean = 100 Exp = 1.391 X/Y/Z = -0.011 0.263 3.754 Errors in X/Y Prediction -0.014 -0.188 um, 1.0 14.4 % Error in Z Prediction -0.011 Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.173 um; position -0.019, 0.192 Drift reached 3.8 A/sec after 5.5 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.15 nm/sec Tracking image shifted 0.002, 0.009 um; position -0.020, 0.180 Running script # 14 1.2 -2.2 unbinned pixels; 0.9 0.1 nm along two shift axes; 0.7 0.5 nm on specimen axes Rate = 4.39223 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.25.24_tomo_04_003_-8.0.tif Record image shifted -0.002, 0.007 um Tracking reference shifted by 19.0,2.8 pixels, -0.002,0.007 um Saved Z = 3 at 2.08 min Tilt = -8.00 Mean = 100 Exp = 1.384 X/Y/Z = -0.019 0.173 3.454 Need regular tracking - Need autofocus Tracking image shifted 0.007, 0.020 um; position -0.027, 0.153 Drift reached 1.9 A/sec after 5.6 sec, going on Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.14 nm/sec Tracking image shifted 0.003, -0.006 um; position -0.030, 0.160 Running script # 14 -1.6 -1.1 unbinned pixels; 0.2 0.7 nm along two shift axes; 0.4 -0.5 nm on specimen axes Rate = 3.3997 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.26.08_tomo_04_004_-6.0.tif Record image shifted -0.001, -0.000 um Tracking reference shifted by -0.3,2.5 pixels, -0.001,-0.000 um Saved Z = 4 at 2.80 min Tilt = -6.00 Mean = 100 Exp = 1.379 X/Y/Z = -0.029 0.160 3.385 Predicted X = -0.005 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = 0.417 StdErr = 0.140 nFit = 3 nDrop = 0 Predicted Z = 3.878 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.012, 0.214 um; position -0.017, 0.204 Drift reached 1.5 A/sec after 5.4 sec, going on Running script # 14 -2.3 -0.3 unbinned pixels; 0.2 0.8 nm along two shift axes; 0.2 -0.8 nm on specimen axes Rate = 4.13967 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.26.37_tomo_04_005_-16.0.tif Record image shifted 0.003, 0.004 um Tracking reference shifted by 11.0,-10.0 pixels, 0.003,0.004 um Saved Z = 5 at 3.30 min Tilt = -16.00 Mean = 99 Exp = 1.395 X/Y/Z = -0.020 0.201 3.878 Errors in X/Y Prediction -0.015 -0.216 um, 1.1 16.5 % Predicted X = -0.026 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.199 StdErr = 0.067 nFit = 3 nDrop = 0 Predicted Z = 4.001 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, 0.026 um; position -0.030, 0.173 Drift reached 3.2 A/sec after 5.6 sec, going on Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.17 nm/sec Tracking image shifted 0.004, 0.007 um; position -0.034, 0.164 Running script # 14 4.4 -0.3 unbinned pixels; 0.6 1.4 nm along two shift axes; -0.0 1.6 nm on specimen axes Rate = 7.75733 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.27.18_tomo_04_006_-18.0.tif Record image shifted -0.004, 0.008 um Tracking reference shifted by 23.8,9.6 pixels, -0.004,0.008 um Saved Z = 6 at 3.97 min Tilt = -18.00 Mean = 99 Exp = 1.406 X/Y/Z = -0.030 0.154 3.914 Errors in X/Y Prediction -0.004 -0.045 um, 0.3 3.4 % Error in Z Prediction -0.086 Predicted X = -0.039 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.148 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.317 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.095 um; position -0.038, 0.243 Drift reached 4.1 A/sec after 5.6 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.12 nm/sec Tracking image shifted 0.001, 0.007 um; position -0.038, 0.234 Running script # 14 0.8 -1.3 unbinned pixels; 0.5 0.1 nm along two shift axes; 0.4 0.3 nm on specimen axes Rate = 2.69747 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.28.03_tomo_04_007_-4.0.tif Record image shifted -0.001, 0.005 um Tracking reference shifted by 15.6,2.5 pixels, -0.001,0.005 um Saved Z = 7 at 4.72 min Tilt = -4.00 Mean = 100 Exp = 1.381 X/Y/Z = -0.038 0.228 3.271 Errors in X/Y Prediction 0.001 0.081 um, 0.1 6.2 % Error in Z Prediction -0.045 Predicted X = -0.047 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.243 StdErr = 0.060 nFit = 3 nDrop = 0 Predicted Z = 3.188 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, 0.030 um; position -0.048, 0.213 Drift reached 0.3 A/sec after 5.5 sec, going on Running script # 14 -2.0 0.0 unbinned pixels; 0.2 0.6 nm along two shift axes; 0.1 -0.7 nm on specimen axes Rate = 3.44917 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.28.33_tomo_04_008_-2.0.tif Record image shifted 0.000, -0.001 um Tracking reference shifted by -1.7,-1.0 pixels, 0.000,-0.001 um Saved Z = 8 at 5.24 min Tilt = -2.00 Mean = 100 Exp = 1.380 X/Y/Z = -0.049 0.214 3.188 Errors in X/Y Prediction -0.002 -0.029 um, 0.1 2.2 % Predicted X = -0.039 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.097 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Z = 4.010 StdErr = 0.033 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, 0.013 um; position -0.042, 0.084 Drift reached 0.9 A/sec after 5.5 sec, going on Running script # 14 -1.8 -0.3 unbinned pixels; 0.1 0.6 nm along two shift axes; 0.2 -0.6 nm on specimen axes Rate = 3.2414 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.29.04_tomo_04_009_-20.0.tif Record image shifted -0.001, 0.004 um Tracking reference shifted by 12.3,1.8 pixels, -0.001,0.004 um Saved Z = 9 at 5.75 min Tilt = -20.00 Mean = 99 Exp = 1.414 X/Y/Z = -0.041 0.081 4.010 Errors in X/Y Prediction -0.002 -0.016 um, 0.2 1.2 % Predicted X = -0.052 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.013 StdErr = 0.028 nFit = 4 nDrop = 0 Predicted Z = 4.101 StdErr = 0.041 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.003, -0.050 um; position -0.048, 0.063 Drift reached 4.0 A/sec after 5.6 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.16 nm/sec Tracking image shifted 0.001, 0.007 um; position -0.050, 0.054 Running script # 14 1.8 -1.0 unbinned pixels; 0.5 0.5 nm along two shift axes; 0.3 0.7 nm on specimen axes Rate = 3.6503 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.29.45_tomo_04_010_-22.0.tif Record image shifted -0.001, 0.011 um Tracking reference shifted by 31.2,-0.2 pixels, -0.001,0.011 um Saved Z = 10 at 6.43 min Tilt = -22.00 Mean = 99 Exp = 1.426 X/Y/Z = -0.049 0.042 4.035 Errors in X/Y Prediction 0.002 0.029 um, 0.2 2.2 % Error in Z Prediction -0.065 Predicted X = -0.058 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.255 StdErr = 0.061 nFit = 3 nDrop = 0 Predicted Z = 3.096 StdErr = 0.045 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.003, 0.002 um; position -0.055, 0.253 Drift reached 3.2 A/sec after 5.5 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.11 nm/sec Tracking image shifted 0.001, 0.007 um; position -0.056, 0.244 Running script # 14 0.8 -0.7 unbinned pixels; 0.3 0.2 nm along two shift axes; 0.2 0.3 nm on specimen axes Rate = 1.87024 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.30.30_tomo_04_011_0.0.tif Record image shifted -0.001, 0.009 um Tracking reference shifted by 25.2,1.8 pixels, -0.001,0.009 um Saved Z = 11 at 7.17 min Tilt = 0.00 Mean = 100 Exp = 1.385 X/Y/Z = -0.055 0.235 3.106 Errors in X/Y Prediction 0.003 -0.020 um, 0.2 1.5 % Error in Z Prediction 0.010 Predicted X = -0.064 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.232 StdErr = 0.026 nFit = 3 nDrop = 0 Predicted Z = 3.010 StdErr = 0.028 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.029 um; position -0.066, 0.262 Drift reached 1.0 A/sec after 5.5 sec, going on Running script # 14 -2.3 0.9 unbinned pixels; 0.6 0.7 nm along two shift axes; -0.2 -0.8 nm on specimen axes Rate = 4.34418 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.31.00_tomo_04_012_2.0.tif Record image shifted -0.000, -0.001 um Tracking reference shifted by -1.6,0.6 pixels, -0.000,-0.001 um Saved Z = 12 at 7.68 min Tilt = 2.00 Mean = 99 Exp = 1.385 X/Y/Z = -0.065 0.264 3.010 Errors in X/Y Prediction -0.001 0.031 um, 0.1 2.4 % Predicted X = -0.060 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.010 StdErr = 0.023 nFit = 5 nDrop = 0 Predicted Z = 4.112 StdErr = 0.025 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.005, 0.002 um; position -0.054, -0.012 Drift reached 0.4 A/sec after 5.5 sec, going on Running script # 14 -0.7 -0.2 unbinned pixels; 0.0 0.2 nm along two shift axes; 0.1 -0.2 nm on specimen axes Rate = 1.20178 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.31.32_tomo_04_013_-24.0.tif Record image shifted -0.001, 0.007 um Tracking reference shifted by 19.2,0.2 pixels, -0.001,0.007 um Saved Z = 13 at 8.20 min Tilt = -24.00 Mean = 99 Exp = 1.444 X/Y/Z = -0.054 -0.018 4.112 Errors in X/Y Prediction 0.006 -0.008 um, 0.4 0.6 % Predicted X = -0.055 StdErr = 0.000 nFit = 4 nDrop = 0 Predicted Y = -0.069 StdErr = 0.017 nFit = 6 nDrop = 0 Predicted Z = 4.183 StdErr = 0.029 nFit = 3 nDrop = 0 Need autofocus Drift reached 1.8 A/sec after 5.1 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.15 nm/sec Running script # 14 2.2 -1.0 unbinned pixels; 0.6 0.6 nm along two shift axes; 0.3 0.8 nm on specimen axes Rate = 4.26582 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.32.07_tomo_04_014_-26.0.tif Record image shifted 0.005, -0.028 um Saved Z = 14 at 8.80 min Tilt = -26.00 Mean = 99 Exp = 1.462 X/Y/Z = -0.060 -0.041 4.129 Errors in X/Y Prediction -0.005 0.027 um, 0.4 2.1 % Error in Z Prediction -0.054 Predicted X = -0.073 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = 0.321 StdErr = 0.018 nFit = 4 nDrop = 0 Predicted Z = 2.919 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, 0.047 um; position -0.075, 0.274 Drift reached 2.1 A/sec after 5.5 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.06 nm/sec Tracking image shifted 0.000, 0.003 um; position -0.075, 0.269 Running script # 14 0.3 0.0 unbinned pixels; 0.0 0.1 nm along two shift axes; -0.0 0.1 nm on specimen axes Rate = 0.58798 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.32.54_tomo_04_015_4.0.tif Record image shifted -0.001, 0.011 um Tracking reference shifted by 32.5,-0.2 pixels, -0.001,0.011 um Saved Z = 15 at 9.56 min Tilt = 4.00 Mean = 99 Exp = 1.394 X/Y/Z = -0.074 0.258 2.933 Errors in X/Y Prediction -0.001 -0.063 um, 0.1 4.8 % Error in Z Prediction 0.014 Predicted X = -0.084 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.285 StdErr = 0.037 nFit = 5 nDrop = 0 Predicted Z = 2.850 StdErr = 0.005 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.004, 0.020 um; position -0.080, 0.265 Drift reached 1.5 A/sec after 5.6 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.10 nm/sec Tracking image shifted 0.000, -0.004 um; position -0.080, 0.270 Running script # 14 -1.6 0.6 unbinned pixels; 0.4 0.5 nm along two shift axes; -0.2 -0.6 nm on specimen axes Rate = 3.04592 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.33.36_tomo_04_016_6.0.tif Record image shifted -0.000, 0.005 um Tracking reference shifted by 15.1,-0.4 pixels, -0.000,0.005 um Saved Z = 16 at 10.28 min Tilt = 6.00 Mean = 99 Exp = 1.401 X/Y/Z = -0.080 0.265 2.835 Errors in X/Y Prediction 0.004 -0.020 um, 0.3 1.5 % Error in Z Prediction -0.015 Predicted X = -0.065 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.079 StdErr = 0.022 nFit = 6 nDrop = 0 Predicted Z = 4.187 StdErr = 0.018 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, -0.006 um; position -0.064, -0.073 Drift reached 0.1 A/sec after 5.5 sec, going on Running script # 14 0.2 0.4 unbinned pixels; 0.1 0.1 nm along two shift axes; -0.1 0.1 nm on specimen axes Rate = 0.69617 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.34.08_tomo_04_017_-28.0.tif Record image shifted -0.001, 0.023 um Tracking reference shifted by 66.2,-2.0 pixels, -0.001,0.023 um Saved Z = 17 at 10.82 min Tilt = -28.00 Mean = 99 Exp = 1.482 X/Y/Z = -0.063 -0.096 4.187 Errors in X/Y Prediction 0.002 -0.018 um, 0.2 1.3 % Predicted X = -0.068 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.135 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 4.241 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.020 um; position -0.066, -0.115 Drift reached 2.6 A/sec after 5.6 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.13 nm/sec Tracking image shifted 0.000, 0.006 um; position -0.066, -0.123 Running script # 14 1.8 -0.3 unbinned pixels; 0.3 0.5 nm along two shift axes; 0.1 0.6 nm on specimen axes Rate = 3.17124 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.34.50_tomo_04_018_-30.0.tif Record image shifted 0.001, 0.016 um Tracking reference shifted by 46.4,-5.5 pixels, 0.001,0.016 um Saved Z = 18 at 11.51 min Tilt = -30.00 Mean = 98 Exp = 1.502 X/Y/Z = -0.067 -0.140 4.255 Errors in X/Y Prediction 0.001 -0.005 um, 0.1 0.4 % Error in Z Prediction 0.014 Predicted X = -0.083 StdErr = 0.001 nFit = 4 nDrop = 0 Predicted Y = 0.254 StdErr = 0.018 nFit = 5 nDrop = 1 Predicted Z = 2.748 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.006, -0.064 um; position -0.077, 0.318 Drift reached 3.2 A/sec after 5.5 sec, going on Running script # 14 2.2 0.2 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.1 0.8 nm on specimen axes Rate = 3.92045 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.35.24_tomo_04_019_8.0.tif Record image shifted -0.001, 0.014 um Tracking reference shifted by 40.3,-1.4 pixels, -0.001,0.014 um Saved Z = 19 at 12.07 min Tilt = 8.00 Mean = 99 Exp = 1.409 X/Y/Z = -0.076 0.301 2.748 Errors in X/Y Prediction 0.008 0.047 um, 0.6 3.6 % Predicted X = -0.068 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = 0.307 StdErr = 0.027 nFit = 6 nDrop = 0 Predicted Z = 2.659 StdErr = 0.011 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.016, 0.064 um; position -0.083, 0.243 Drift reached 1.0 A/sec after 5.5 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.05 nm/sec Tracking image shifted 0.001, -0.002 um; position -0.084, 0.245 Running script # 14 -2.0 0.8 unbinned pixels; 0.5 0.6 nm along two shift axes; -0.2 -0.7 nm on specimen axes Rate = 3.80522 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.36.06_tomo_04_020_10.0.tif Record image shifted 0.000, 0.002 um Tracking reference shifted by 6.0,-1.5 pixels, 0.000,0.002 um Saved Z = 20 at 12.78 min Tilt = 10.00 Mean = 99 Exp = 1.417 X/Y/Z = -0.084 0.244 2.621 Errors in X/Y Prediction -0.016 -0.063 um, 1.2 4.8 % Error in Z Prediction -0.037 Predicted X = -0.070 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.183 StdErr = 0.027 nFit = 5 nDrop = 0 Predicted Z = 4.304 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.010 um; position -0.068, -0.192 Drift reached 1.0 A/sec after 5.7 sec, going on Running script # 14 1.8 0.1 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.1 0.6 nm on specimen axes Rate = 3.20012 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.36.39_tomo_04_021_-32.0.tif Record image shifted -0.001, 0.016 um Tracking reference shifted by 44.6,-0.3 pixels, -0.001,0.016 um Saved Z = 21 at 13.33 min Tilt = -32.00 Mean = 98 Exp = 1.525 X/Y/Z = -0.067 -0.209 4.304 Errors in X/Y Prediction 0.003 -0.027 um, 0.2 2.0 % Predicted X = -0.070 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.286 StdErr = 0.016 nFit = 5 nDrop = 0 Predicted Z = 4.359 StdErr = 0.024 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, -0.057 um; position -0.070, -0.229 Drift reached 3.1 A/sec after 5.7 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.13 nm/sec Tracking image shifted 0.000, 0.005 um; position -0.070, -0.236 Running script # 14 3.3 -0.1 unbinned pixels; 0.4 1.1 nm along two shift axes; -0.1 1.2 nm on specimen axes Rate = 5.80825 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.37.26_tomo_04_022_-34.0.tif Record image shifted 0.001, 0.013 um Tracking reference shifted by 36.8,-7.0 pixels, 0.001,0.013 um Saved Z = 22 at 14.11 min Tilt = -34.00 Mean = 98 Exp = 1.559 X/Y/Z = -0.071 -0.250 4.361 Errors in X/Y Prediction -0.001 0.036 um, 0.1 2.7 % Error in Z Prediction 0.002 Predicted X = -0.087 StdErr = 0.014 nFit = 4 nDrop = 0 Predicted Y = 0.234 StdErr = 0.045 nFit = 6 nDrop = 0 Predicted Z = 2.534 StdErr = 0.021 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, -0.029 um; position -0.084, 0.263 Drift reached 1.4 A/sec after 5.5 sec, going on Running script # 14 -3.2 1.9 unbinned pixels; 1.0 0.8 nm along two shift axes; -0.6 -1.2 nm on specimen axes Rate = 6.5027 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.38.01_tomo_04_023_12.0.tif Record image shifted -0.000, 0.007 um Tracking reference shifted by 19.6,-1.1 pixels, -0.000,0.007 um Saved Z = 23 at 14.68 min Tilt = 12.00 Mean = 99 Exp = 1.433 X/Y/Z = -0.083 0.258 2.534 Errors in X/Y Prediction 0.004 0.024 um, 0.3 1.8 % Predicted X = -0.089 StdErr = 0.013 nFit = 4 nDrop = 0 Predicted Y = 0.234 StdErr = 0.046 nFit = 6 nDrop = 0 Predicted Z = 2.437 StdErr = 0.024 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.062 um; position -0.095, 0.172 Drift reached 1.0 A/sec after 5.6 sec, going on Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.08 nm/sec Tracking image shifted 0.000, 0.002 um; position -0.094, 0.171 Running script # 14 0.2 0.6 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 0.0 nm on specimen axes Rate = 1.02867 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.38.44_tomo_04_024_14.0.tif Record image shifted -0.000, 0.015 um Tracking reference shifted by 42.8,-2.8 pixels, -0.000,0.015 um Saved Z = 24 at 15.40 min Tilt = 14.00 Mean = 99 Exp = 1.449 X/Y/Z = -0.094 0.156 2.488 Errors in X/Y Prediction -0.005 -0.079 um, 0.4 6.0 % Error in Z Prediction 0.050 Predicted X = -0.073 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.306 StdErr = 0.020 nFit = 5 nDrop = 0 Predicted Z = 4.422 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, 0.047 um; position -0.071, -0.353 Drift reached 1.4 A/sec after 5.7 sec, going on Running script # 14 1.5 0.4 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.2 0.5 nm on specimen axes Rate = 2.72297 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.39.17_tomo_04_025_-36.0.tif Record image shifted -0.000, 0.017 um Tracking reference shifted by 48.4,-3.7 pixels, -0.000,0.017 um Saved Z = 25 at 15.96 min Tilt = -36.00 Mean = 98 Exp = 1.592 X/Y/Z = -0.070 -0.371 4.422 Errors in X/Y Prediction 0.002 -0.065 um, 0.2 5.0 % Predicted X = -0.073 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.474 StdErr = 0.043 nFit = 5 nDrop = 0 Predicted Z = 4.480 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.084 um; position -0.074, -0.390 Drift reached 3.4 A/sec after 5.7 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.12 nm/sec Tracking image shifted 0.000, 0.005 um; position -0.074, -0.397 Running script # 14 1.5 -0.1 unbinned pixels; 0.2 0.5 nm along two shift axes; 0.0 0.5 nm on specimen axes Rate = 2.64753 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.39.58_tomo_04_026_-38.0.tif Record image shifted 0.000, 0.017 um Tracking reference shifted by 49.4,-5.0 pixels, 0.000,0.017 um Saved Z = 26 at 16.65 min Tilt = -38.00 Mean = 97 Exp = 1.628 X/Y/Z = -0.074 -0.415 4.454 Errors in X/Y Prediction -0.001 0.059 um, 0.1 4.5 % Error in Z Prediction -0.025 Predicted X = -0.097 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = 0.082 StdErr = 0.054 nFit = 6 nDrop = 0 Predicted Z = 2.380 StdErr = 0.038 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted -0.005, -0.089 um; position -0.092, 0.170 Drift reached 5.6 A/sec after 5.5 sec, going on Running script # 14 -9.0 2.3 unbinned pixels; 1.8 2.7 nm along two shift axes; -0.5 -3.2 nm on specimen axes Rate = 16.2302 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.40.34_tomo_04_027_16.0.tif Record image shifted -0.001, -0.002 um Tracking reference shifted by -6.3,4.1 pixels, -0.001,-0.002 um Saved Z = 27 at 17.23 min Tilt = 16.00 Mean = 98 Exp = 1.464 X/Y/Z = -0.090 0.179 2.380 Errors in X/Y Prediction 0.008 0.097 um, 0.6 7.5 % Predicted X = -0.095 StdErr = 0.011 nFit = 3 nDrop = 0 Predicted Y = 0.108 StdErr = 0.077 nFit = 6 nDrop = 0 Predicted Z = 2.289 StdErr = 0.043 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.009, -0.006 um; position -0.086, 0.114 Drift reached 6.3 A/sec after 5.5 sec, going on Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.20 nm/sec Tracking image shifted -0.006, 0.012 um; position -0.079, 0.098 Running script # 14 0.2 1.8 unbinned pixels; 0.6 0.3 nm along two shift axes; -0.6 0.0 nm on specimen axes Rate = 3.14598 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.41.16_tomo_04_028_18.0.tif Record image shifted 0.001, 0.026 um Tracking reference shifted by 74.3,-9.2 pixels, 0.001,0.026 um Saved Z = 28 at 17.94 min Tilt = 18.00 Mean = 99 Exp = 1.488 X/Y/Z = -0.079 0.072 2.354 Errors in X/Y Prediction 0.016 -0.036 um, 1.2 2.8 % Error in Z Prediction 0.065 Predicted X = -0.075 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = -0.505 StdErr = 0.059 nFit = 5 nDrop = 0 Predicted Z = 4.506 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, 0.027 um; position -0.075, -0.532 Drift reached 1.8 A/sec after 6.0 sec, going on Running script # 14 3.9 0.1 unbinned pixels; 0.4 1.3 nm along two shift axes; -0.1 1.4 nm on specimen axes Rate = 6.90317 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.41.56_tomo_04_029_-40.0.tif Record image shifted -0.000, 0.020 um Tracking reference shifted by 55.8,-4.0 pixels, -0.000,0.020 um Saved Z = 29 at 18.60 min Tilt = -40.00 Mean = 97 Exp = 1.671 X/Y/Z = -0.074 -0.555 4.506 Errors in X/Y Prediction 0.001 -0.049 um, 0.1 3.8 % Predicted X = -0.076 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -0.677 StdErr = 0.066 nFit = 5 nDrop = 0 Predicted Z = 4.556 StdErr = 0.009 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.003, -0.089 um; position -0.079, -0.588 Drift reached 3.4 A/sec after 5.9 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.16 nm/sec Tracking image shifted 0.000, 0.006 um; position -0.078, -0.596 Running script # 14 2.1 -0.4 unbinned pixels; 0.4 0.7 nm along two shift axes; 0.1 0.8 nm on specimen axes Rate = 3.82537 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.42.38_tomo_04_030_-42.0.tif Record image shifted 0.001, 0.021 um Tracking reference shifted by 58.0,-7.4 pixels, 0.001,0.021 um Saved Z = 30 at 19.31 min Tilt = -42.00 Mean = 97 Exp = 1.722 X/Y/Z = -0.079 -0.617 4.535 Errors in X/Y Prediction -0.003 0.060 um, 0.2 4.6 % Error in Z Prediction -0.021 Predicted X = -0.081 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.706 StdErr = 0.069 nFit = 5 nDrop = 0 Predicted Z = 4.581 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, -0.010 um; position -0.083, -0.696 Drift reached 0.4 A/sec after 5.9 sec, going on Running script # 14 0.5 0.2 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.1 0.2 nm on specimen axes Rate = 1.0352 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.43.08_tomo_04_031_-44.0.tif Record image shifted -0.000, 0.021 um Tracking reference shifted by 60.3,-4.0 pixels, -0.000,0.021 um Saved Z = 31 at 19.81 min Tilt = -44.00 Mean = 97 Exp = 1.776 X/Y/Z = -0.083 -0.718 4.581 Errors in X/Y Prediction -0.001 -0.012 um, 0.1 0.9 % Predicted X = -0.073 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.003 StdErr = 0.100 nFit = 5 nDrop = 0 Predicted Z = 2.259 StdErr = 0.044 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.021, -0.100 um; position -0.094, 0.097 Drift reached 3.6 A/sec after 5.5 sec, going on Running script # 14 -6.3 2.6 unbinned pixels; 1.6 1.8 nm along two shift axes; -0.7 -2.3 nm on specimen axes Rate = 11.90196 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.43.44_tomo_04_032_20.0.tif Record image shifted -0.002, 0.006 um Tracking reference shifted by 18.8,5.2 pixels, -0.002,0.006 um Saved Z = 32 at 20.41 min Tilt = 20.00 Mean = 98 Exp = 1.505 X/Y/Z = -0.091 0.095 2.259 Errors in X/Y Prediction -0.018 0.098 um, 1.4 7.5 % Predicted X = -0.088 StdErr = 0.016 nFit = 3 nDrop = 0 Predicted Y = 0.088 StdErr = 0.103 nFit = 5 nDrop = 0 Predicted Z = 2.180 StdErr = 0.049 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, 0.013 um; position -0.086, 0.075 Drift reached 4.4 A/sec after 6.4 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.10 nm/sec Tracking image shifted -0.005, 0.007 um; position -0.079, 0.066 Running script # 14 -0.7 1.3 unbinned pixels; 0.5 0.1 nm along two shift axes; -0.4 -0.3 nm on specimen axes Rate = 2.56954 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.44.28_tomo_04_033_22.0.tif Record image shifted 0.002, 0.022 um Tracking reference shifted by 60.5,-11.9 pixels, 0.002,0.022 um Saved Z = 33 at 21.15 min Tilt = 22.00 Mean = 98 Exp = 1.535 X/Y/Z = -0.081 0.045 2.189 Errors in X/Y Prediction 0.006 -0.043 um, 0.5 3.3 % Error in Z Prediction 0.009 Predicted X = -0.086 StdErr = 0.015 nFit = 3 nDrop = 0 Predicted Y = 0.009 StdErr = 0.097 nFit = 5 nDrop = 0 Predicted Z = 2.120 StdErr = 0.019 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.008, -0.038 um; position -0.094, 0.047 Drift reached 7.5 A/sec after 5.7 sec, going on Running script # 14 -8.5 5.3 unbinned pixels; 2.7 2.2 nm along two shift axes; -1.6 -3.1 nm on specimen axes Rate = 17.49898 A/sec -7.3 2.7 unbinned pixels; 1.8 2.1 nm along two shift axes; -0.7 -2.7 nm on specimen axes Rate = 13.7112 A/sec Drift is low enough after shot 2 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.45.05_tomo_04_034_24.0.tif Record image shifted -0.004, 0.000 um Tracking reference shifted by 1.6,12.1 pixels, -0.004,0.000 um Saved Z = 34 at 21.77 min Tilt = 24.00 Mean = 98 Exp = 1.566 X/Y/Z = -0.085 0.057 2.120 Errors in X/Y Prediction 0.001 0.047 um, 0.1 3.6 % Predicted X = -0.087 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.822 StdErr = 0.065 nFit = 5 nDrop = 0 Predicted Z = 4.624 StdErr = 0.010 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, -0.004 um; position -0.082, -0.818 Drift reached 2.7 A/sec after 6.1 sec, going on Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.12 nm/sec Tracking image shifted 0.000, 0.007 um; position -0.082, -0.826 Running script # 14 1.7 -0.1 unbinned pixels; 0.2 0.5 nm along two shift axes; -0.0 0.6 nm on specimen axes Rate = 2.93432 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.45.54_tomo_04_035_-46.0.tif Record image shifted 0.001, 0.025 um Tracking reference shifted by 69.7,-9.0 pixels, 0.001,0.025 um Saved Z = 35 at 22.59 min Tilt = -46.00 Mean = 96 Exp = 1.837 X/Y/Z = -0.083 -0.852 4.610 Errors in X/Y Prediction 0.004 -0.030 um, 0.3 2.3 % Error in Z Prediction -0.014 Predicted X = -0.086 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.955 StdErr = 0.063 nFit = 5 nDrop = 0 Predicted Z = 4.650 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.007, -0.013 um; position -0.093, -0.942 Drift reached 0.3 A/sec after 6.2 sec, going on Running script # 14 0.3 0.4 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.1 0.1 nm on specimen axes Rate = 0.90489 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.46.25_tomo_04_036_-48.0.tif Record image shifted -0.001, 0.025 um Tracking reference shifted by 70.0,-3.5 pixels, -0.001,0.025 um Saved Z = 36 at 23.10 min Tilt = -48.00 Mean = 96 Exp = 1.910 X/Y/Z = -0.092 -0.967 4.650 Errors in X/Y Prediction -0.006 -0.012 um, 0.5 0.9 % Predicted X = -0.100 StdErr = 0.007 nFit = 4 nDrop = 0 Predicted Y = -1.129 StdErr = 0.031 nFit = 5 nDrop = 1 Predicted Z = 4.689 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.007, -0.097 um; position -0.093, -1.032 Drift reached 2.8 A/sec after 6.3 sec, going on Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.11 nm/sec Tracking image shifted 0.000, 0.004 um; position -0.092, -1.038 Running script # 14 1.5 -0.4 unbinned pixels; 0.3 0.4 nm along two shift axes; 0.1 0.5 nm on specimen axes Rate = 2.66058 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.47.09_tomo_04_037_-50.0.tif Record image shifted 0.001, 0.028 um Tracking reference shifted by 78.6,-10.6 pixels, 0.001,0.028 um Saved Z = 37 at 23.84 min Tilt = -50.00 Mean = 95 Exp = 1.993 X/Y/Z = -0.094 -1.067 4.685 Errors in X/Y Prediction 0.006 0.063 um, 0.5 4.8 % Error in Z Prediction -0.003 Predicted X = -0.100 StdErr = 0.005 nFit = 3 nDrop = 0 Predicted Y = -1.178 StdErr = 0.028 nFit = 5 nDrop = 0 Predicted Z = 4.725 StdErr = 0.003 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, -0.013 um; position -0.101, -1.166 Drift reached 1.5 A/sec after 11.5 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.07 nm/sec Tracking image shifted -0.000, 0.003 um; position -0.100, -1.170 Running script # 14 0.8 -0.1 unbinned pixels; 0.1 0.3 nm along two shift axes; 0.0 0.3 nm on specimen axes Rate = 1.47496 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.48.00_tomo_04_038_-52.0.tif Record image shifted 0.000, 0.028 um Tracking reference shifted by 80.5,-8.3 pixels, 0.000,0.028 um Saved Z = 38 at 24.68 min Tilt = -52.00 Mean = 95 Exp = 2.092 X/Y/Z = -0.100 -1.199 4.702 Errors in X/Y Prediction -0.000 -0.020 um, 0.0 1.6 % Error in Z Prediction -0.023 Predicted X = -0.080 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = 0.091 StdErr = 0.076 nFit = 5 nDrop = 0 Predicted Z = 2.045 StdErr = 0.023 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, 0.033 um; position -0.084, 0.058 Drift reached 9.3 A/sec after 5.8 sec, going on Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.34 nm/sec Tracking image shifted -0.009, -0.024 um; position -0.073, 0.087 Running script # 14 -1.1 3.2 unbinned pixels; 1.2 0.0 nm along two shift axes; -1.1 -0.5 nm on specimen axes Rate = 5.86677 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.48.49_tomo_04_039_26.0.tif Record image shifted 0.005, 0.031 um Tracking reference shifted by 88.0,-20.6 pixels, 0.005,0.031 um Saved Z = 39 at 25.50 min Tilt = 26.00 Mean = 97 Exp = 1.602 X/Y/Z = -0.076 0.056 2.015 Errors in X/Y Prediction 0.004 -0.035 um, 0.3 2.7 % Error in Z Prediction -0.031 Predicted X = -0.075 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Y = 0.050 StdErr = 0.052 nFit = 5 nDrop = 0 Predicted Z = 1.932 StdErr = 0.007 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.012, 0.062 um; position -0.088, -0.012 Drift reached 4.5 A/sec after 5.7 sec, going on Running script # 14 -1.2 1.9 unbinned pixels; 0.8 0.2 nm along two shift axes; -0.6 -0.5 nm on specimen axes Rate = 4.01863 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.49.21_tomo_04_040_28.0.tif Record image shifted -0.001, 0.028 um Tracking reference shifted by 80.6,-4.6 pixels, -0.001,0.028 um Saved Z = 40 at 26.03 min Tilt = 28.00 Mean = 98 Exp = 1.644 X/Y/Z = -0.084 -0.042 1.932 Errors in X/Y Prediction -0.009 -0.092 um, 0.7 7.1 % Predicted X = -0.081 StdErr = 0.013 nFit = 3 nDrop = 0 Predicted Y = -0.091 StdErr = 0.083 nFit = 5 nDrop = 0 Predicted Z = 1.847 StdErr = 0.008 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.025, 0.034 um; position -0.106, -0.125 Drift reached 5.3 A/sec after 6.0 sec, going on Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.25 nm/sec Tracking image shifted -0.003, -0.011 um; position -0.101, -0.111 Running script # 14 -3.7 2.2 unbinned pixels; 1.2 1.0 nm along two shift axes; -0.7 -1.4 nm on specimen axes Rate = 7.60797 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.50.06_tomo_04_041_30.0.tif Record image shifted 0.001, 0.012 um Tracking reference shifted by 33.5,-6.9 pixels, 0.001,0.012 um Saved Z = 41 at 26.78 min Tilt = 30.00 Mean = 97 Exp = 1.683 X/Y/Z = -0.102 -0.122 1.897 Errors in X/Y Prediction -0.021 -0.031 um, 1.6 2.4 % Error in Z Prediction 0.050 Predicted X = -0.114 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = -0.271 StdErr = 0.043 nFit = 5 nDrop = 0 Predicted Z = 1.814 StdErr = 0.036 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.012, -0.145 um; position -0.101, -0.126 Drift reached 4.1 A/sec after 5.9 sec, going on Running script # 14 -4.4 2.7 unbinned pixels; 1.4 1.1 nm along two shift axes; -0.8 -1.6 nm on specimen axes Rate = 9.0793 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.50.38_tomo_04_042_32.0.tif Record image shifted -0.003, 0.020 um Tracking reference shifted by 58.1,4.5 pixels, -0.003,0.020 um Saved Z = 42 at 27.31 min Tilt = 32.00 Mean = 97 Exp = 1.732 X/Y/Z = -0.095 -0.143 1.814 Errors in X/Y Prediction 0.019 0.128 um, 1.4 9.8 % Predicted X = -0.104 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -1.304 StdErr = 0.028 nFit = 5 nDrop = 0 Predicted Z = 4.745 StdErr = 0.014 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.021 um; position -0.102, -1.283 Drift reached 1.7 A/sec after 6.6 sec, going on Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.09 nm/sec Tracking image shifted -0.000, 0.005 um; position -0.102, -1.290 Running script # 14 1.6 0.1 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.1 0.6 nm on specimen axes Rate = 2.81276 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.51.29_tomo_04_043_-54.0.tif Record image shifted 0.001, 0.032 um Tracking reference shifted by 89.8,-9.4 pixels, 0.001,0.032 um Saved Z = 43 at 28.17 min Tilt = -54.00 Mean = 94 Exp = 2.208 X/Y/Z = -0.102 -1.322 4.733 Errors in X/Y Prediction 0.002 -0.018 um, 0.1 1.4 % Error in Z Prediction -0.012 Predicted X = -0.107 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = -1.457 StdErr = 0.019 nFit = 5 nDrop = 0 Predicted Z = 4.766 StdErr = 0.013 nFit = 4 nDrop = 0 Drift reached 0.1 A/sec after 6.3 sec, going on Running script # 14 0.5 0.1 unbinned pixels; 0.0 0.2 nm along two shift axes; -0.0 0.2 nm on specimen axes Rate = 0.96864 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.52.00_tomo_04_044_-56.0.tif Record image shifted 0.010, -0.053 um Saved Z = 44 at 28.69 min Tilt = -56.00 Mean = 93 Exp = 2.341 X/Y/Z = -0.118 -1.404 4.766 Errors in X/Y Prediction -0.010 0.053 um, 0.8 4.1 % Predicted X = -0.124 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Y = -1.509 StdErr = 0.039 nFit = 5 nDrop = 0 Predicted Z = 4.796 StdErr = 0.015 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.014, -0.106 um; position -0.110, -1.403 Drift reached 1.8 A/sec after 6.9 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.09 nm/sec Tracking image shifted -0.000, 0.005 um; position -0.109, -1.409 Running script # 14 0.9 0.0 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.0 0.3 nm on specimen axes Rate = 1.55934 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.52.48_tomo_04_045_-58.0.tif Record image shifted 0.001, 0.070 um Tracking reference shifted by 198.1,-20.7 pixels, 0.001,0.070 um Saved Z = 45 at 29.49 min Tilt = -58.00 Mean = 93 Exp = 2.509 X/Y/Z = -0.110 -1.480 4.810 Errors in X/Y Prediction 0.014 0.029 um, 1.1 2.2 % Error in Z Prediction 0.014 Predicted X = -0.107 StdErr = 0.024 nFit = 4 nDrop = 0 Predicted Y = -1.526 StdErr = 0.014 nFit = 5 nDrop = 1 Predicted Z = 4.837 StdErr = 0.016 nFit = 4 nDrop = 0 Need regular tracking - Tracking image shifted 0.021, 0.011 um; position -0.127, -1.537 Drift reached 0.5 A/sec after 7.3 sec, going on Running script # 14 0.6 0.6 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.2 nm on specimen axes Rate = 1.48301 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.53.27_tomo_04_046_-60.0.tif Record image shifted 0.000, 0.041 um Tracking reference shifted by 114.8,-10.5 pixels, 0.000,0.041 um Saved Z = 46 at 30.15 min Tilt = -60.00 Mean = 92 Exp = 2.689 X/Y/Z = -0.127 -1.577 4.837 Errors in X/Y Prediction -0.020 -0.051 um, 1.5 3.9 % Predicted X = -0.132 StdErr = 0.032 nFit = 4 nDrop = 0 Predicted Y = -1.629 StdErr = 0.027 nFit = 6 nDrop = 0 Predicted Z = 4.869 StdErr = 0.019 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.008, -0.043 um; position -0.124, -1.586 Drift reached 1.1 A/sec after 7.5 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.03 nm/sec Tracking image shifted 0.003, 0.001 um; position -0.126, -1.588 Running script # 14 0.2 -0.1 unbinned pixels; 0.1 0.1 nm along two shift axes; 0.0 0.1 nm on specimen axes Rate = 0.49394 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.54.18_tomo_04_047_-62.0.tif Record image shifted -0.001, 0.050 um Tracking reference shifted by 142.4,-10.2 pixels, -0.001,0.050 um Saved Z = 47 at 31.00 min Tilt = -62.00 Mean = 91 Exp = 2.921 X/Y/Z = -0.125 -1.638 4.873 Errors in X/Y Prediction 0.007 -0.009 um, 0.6 0.7 % Error in Z Prediction 0.004 Predicted X = -0.104 StdErr = 0.019 nFit = 3 nDrop = 0 Predicted Y = -0.224 StdErr = 0.083 nFit = 5 nDrop = 0 Predicted Z = 1.741 StdErr = 0.042 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.014, -0.094 um; position -0.090, -0.130 Drift reached 4.0 A/sec after 5.9 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.20 nm/sec Tracking image shifted -0.007, -0.010 um; position -0.080, -0.119 Running script # 14 -4.8 1.7 unbinned pixels; 1.1 1.4 nm along two shift axes; -0.4 -1.7 nm on specimen axes Rate = 8.94647 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.55.10_tomo_04_048_34.0.tif Record image shifted 0.005, 0.026 um Tracking reference shifted by 71.6,-20.4 pixels, 0.005,0.026 um Saved Z = 48 at 31.84 min Tilt = 34.00 Mean = 96 Exp = 1.791 X/Y/Z = -0.085 -0.143 1.678 Errors in X/Y Prediction 0.019 0.081 um, 1.5 6.2 % Error in Z Prediction -0.064 Predicted X = -0.077 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.101 StdErr = 0.016 nFit = 5 nDrop = 0 Predicted Z = 1.610 StdErr = 0.065 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.016, 0.162 um; position -0.092, -0.263 Drift reached 3.4 A/sec after 6.1 sec, going on Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.13 nm/sec Tracking image shifted 0.002, -0.006 um; position -0.093, -0.255 Running script # 14 -1.5 1.1 unbinned pixels; 0.5 0.4 nm along two shift axes; -0.3 -0.6 nm on specimen axes Rate = 3.27913 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.55.55_tomo_04_049_36.0.tif Record image shifted 0.001, 0.025 um Tracking reference shifted by 70.6,-8.1 pixels, 0.001,0.025 um Saved Z = 49 at 32.61 min Tilt = 36.00 Mean = 96 Exp = 1.865 X/Y/Z = -0.094 -0.279 1.669 Errors in X/Y Prediction -0.017 -0.178 um, 1.3 13.7 % Error in Z Prediction 0.058 Predicted X = -0.090 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Y = -0.340 StdErr = 0.107 nFit = 5 nDrop = 0 Predicted Z = 1.570 StdErr = 0.055 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.017, -0.035 um; position -0.107, -0.305 Drift reached 3.2 A/sec after 6.1 sec, going on Measured defocus = -4.10 microns changed by 0.10 to target drift = 0.32 nm/sec Tracking image shifted -0.006, -0.013 um; position -0.100, -0.290 Running script # 14 -3.0 1.6 unbinned pixels; 0.9 0.8 nm along two shift axes; -0.5 -1.1 nm on specimen axes Rate = 5.86859 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.56.47_tomo_04_050_38.0.tif Record image shifted 0.000, 0.025 um Tracking reference shifted by 71.2,-7.2 pixels, 0.000,0.025 um Saved Z = 50 at 33.46 min Tilt = 38.00 Mean = 96 Exp = 1.941 X/Y/Z = -0.100 -0.314 1.657 Errors in X/Y Prediction -0.010 0.026 um, 0.8 2.0 % Error in Z Prediction 0.088 Predicted X = -0.108 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.439 StdErr = 0.090 nFit = 5 nDrop = 0 Predicted Z = 1.557 StdErr = 0.085 nFit = 4 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.005, -0.084 um; position -0.103, -0.355 Drift reached 2.7 A/sec after 6.4 sec, going on Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.10 nm/sec Tracking image shifted -0.003, -0.006 um; position -0.098, -0.349 Running script # 14 -1.1 0.9 unbinned pixels; 0.4 0.3 nm along two shift axes; -0.3 -0.4 nm on specimen axes Rate = 2.58777 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.57.34_tomo_04_051_40.0.tif Record image shifted 0.000, 0.029 um Tracking reference shifted by 82.4,-7.7 pixels, 0.000,0.029 um Saved Z = 51 at 34.24 min Tilt = 40.00 Mean = 95 Exp = 2.027 X/Y/Z = -0.098 -0.377 1.516 Errors in X/Y Prediction 0.010 0.062 um, 0.7 4.7 % Error in Z Prediction -0.041 Predicted X = -0.093 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = -0.456 StdErr = 0.094 nFit = 5 nDrop = 0 Predicted Z = 1.468 StdErr = 0.069 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.013, -0.056 um; position -0.105, -0.400 Drift reached 3.0 A/sec after 6.5 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.20 nm/sec Tracking image shifted -0.005, -0.010 um; position -0.100, -0.387 Running script # 14 -4.0 1.6 unbinned pixels; 1.0 1.1 nm along two shift axes; -0.4 -1.4 nm on specimen axes Rate = 7.49198 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.58.21_tomo_04_052_42.0.tif Record image shifted 0.001, 0.029 um Tracking reference shifted by 80.7,-8.9 pixels, 0.001,0.029 um Saved Z = 52 at 35.04 min Tilt = 42.00 Mean = 95 Exp = 2.124 X/Y/Z = -0.100 -0.415 1.430 Errors in X/Y Prediction -0.008 0.041 um, 0.6 3.2 % Error in Z Prediction -0.038 Predicted X = -0.137 StdErr = 0.029 nFit = 4 nDrop = 0 Predicted Y = -1.699 StdErr = 0.024 nFit = 6 nDrop = 0 Predicted Z = 4.910 StdErr = 0.006 nFit = 4 nDrop = 1 Need regular tracking - Need autofocus Tracking image shifted -0.002, -0.060 um; position -0.135, -1.639 Trimming A by 20 220 105 20, reference by 21 225 115 20 Drift reached 2.4 A/sec after 7.9 sec, going on Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.07 nm/sec Tracking image shifted -0.000, 0.004 um; position -0.136, -1.644 Running script # 14 Trimming A by 21 225 115 20, reference by 21 225 115 20 Trimming A by 21 225 115 20, reference by 21 225 115 20 0.8 0.5 unbinned pixels; 0.1 0.3 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.7513 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_13.59.20_tomo_04_053_-64.0.tif Record image shifted -0.002, 0.055 um Tracking reference shifted by 156.4,-8.3 pixels, -0.002,0.055 um Saved Z = 53 at 36.05 min Tilt = -64.00 Mean = 90 Exp = 3.210 X/Y/Z = -0.134 -1.700 4.872 Errors in X/Y Prediction 0.003 -0.001 um, 0.2 0.1 % Error in Z Prediction -0.038 Predicted X = -0.131 StdErr = 0.019 nFit = 4 nDrop = 0 Predicted Y = -1.762 StdErr = 0.019 nFit = 6 nDrop = 0 Predicted Z = 4.920 StdErr = 0.020 nFit = 5 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.005, -0.067 um; position -0.136, -1.694 Trimming A by 20 245 130 20, reference by 20 245 130 20 Drift reached 0.5 A/sec after 8.5 sec, going on Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.02 nm/sec Tracking image shifted -0.001, -0.002 um; position -0.134, -1.692 Running script # 14 Trimming A by 20 245 130 20, reference by 20 245 130 20 Trimming A by 20 245 130 20, reference by 20 245 130 20 -0.1 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; 0.0 -0.0 nm on specimen axes Rate = 0.22704 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 30 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_14.00.17_tomo_04_054_-66.0.tif Record image shifted 0.000, 0.053 um Tracking reference shifted by 150.2,-14.3 pixels, 0.000,0.053 um Saved Z = 54 at 37.00 min Tilt = -66.00 Mean = 89 Exp = 3.584 X/Y/Z = -0.135 -1.745 4.943 Errors in X/Y Prediction -0.004 0.017 um, 0.3 1.3 % Error in Z Prediction 0.023 Predicted X = -0.142 StdErr = 0.012 nFit = 4 nDrop = 0 Predicted Y = -1.784 StdErr = 0.016 nFit = 6 nDrop = 0 Predicted Z = 4.962 StdErr = 0.024 nFit = 5 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, -0.055 um; position -0.139, -1.729 Errors in X/Y Prediction 0.007 0.039 um, 0.6 3.0 % Error in Z Prediction -0.019 Need regular tracking - Need autofocus Tracking image shifted 0.007, 0.042 um; position -0.107, -0.457 Drift reached 4.1 A/sec after 6.7 sec, going on Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.25 nm/sec Tracking image shifted -0.005, -0.012 um; position -0.101, -0.442 Running script # 14 -5.2 1.0 unbinned pixels; 0.9 1.6 nm along two shift axes; -0.2 -1.8 nm on specimen axes Rate = 9.24292 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_14.01.35_tomo_04_055_44.0.tif Record image shifted 0.001, 0.033 um Tracking reference shifted by 92.4,-9.5 pixels, 0.001,0.033 um Saved Z = 55 at 38.27 min Tilt = 44.00 Mean = 94 Exp = 2.240 X/Y/Z = -0.101 -0.473 1.367 Need regular tracking - Need autofocus Tracking image shifted 0.008, 0.046 um; position -0.108, -0.519 Drift reached 2.4 A/sec after 7.0 sec, going on Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.10 nm/sec Tracking image shifted 0.000, -0.006 um; position -0.108, -0.511 Running script # 14 -1.3 0.6 unbinned pixels; 0.4 0.3 nm along two shift axes; -0.2 -0.5 nm on specimen axes Rate = 2.5062 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_14.02.25_tomo_04_056_46.0.tif Record image shifted 0.001, 0.035 um Tracking reference shifted by 99.2,-11.7 pixels, 0.001,0.035 um Saved Z = 56 at 39.11 min Tilt = 46.00 Mean = 94 Exp = 2.390 X/Y/Z = -0.109 -0.546 1.305 Predicted X = -0.117 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.619 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 1.243 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.029 um; position -0.118, -0.590 Drift reached 1.8 A/sec after 7.2 sec, going on Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.10 nm/sec Tracking image shifted -0.004, -0.006 um; position -0.113, -0.583 Running script # 14 -1.7 1.7 unbinned pixels; 0.8 0.4 nm along two shift axes; -0.5 -0.6 nm on specimen axes Rate = 4.19179 A/sec Drift is low enough after shot 1 Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_14.03.17_tomo_04_057_48.0.tif Record image shifted 0.000, 0.035 um Tracking reference shifted by 99.5,-10.0 pixels, 0.000,0.035 um Saved Z = 57 at 39.97 min Tilt = 48.00 Mean = 93 Exp = 2.550 X/Y/Z = -0.113 -0.618 1.244 2021/01/12 14:03 D:\Users\Ben\210112_Titan2_Jans_cells\SEM\tomo_04.st 0 58 3838 3710 1651 -10.0 48.0 2.00 REG 40.0 1x1 K2-0001 42000 2 0.35 LD-DS 20.0 0.0 Angle = -4.40, increment = 0.60, shift = 52.3 0.0 pixels, 0.119 um Y Angle = -3.20, increment = 1.80, shift = 155.7 14.5 pixels, 0.357 um Y Angle = 0.41, increment = 5.41, shift = 521.4 56.7 pixels, 1.197 um Y Angle = 3.00, increment = 8.00, shift = 105.9 93.8 pixels, 0.264 um Y Rough eucentricity: changing Z by -1.90 to -205.56, finished. Angle = -4.40, increment = 0.60, shift = 38.5 -0.3 pixels, 0.087 um Y Angle = -3.20, increment = 1.80, shift = 120.4 9.7 pixels, 0.276 um Y Angle = 0.41, increment = 5.41, shift = 406.9 43.1 pixels, 0.934 um Y Angle = 3.00, increment = 8.00, shift = 623.2 69.1 pixels, 1.431 um Y Rough eucentricity: changing Z by -10.26 to -215.81, finished. Angle = -4.40, increment = 0.60, shift = -7.4 -2.1 pixels, -0.017 um Y Angle = -3.20, increment = 1.80, shift = -5.3 -1.8 pixels, -0.012 um Y Angle = 0.41, increment = 5.41, shift = -23.4 -7.9 pixels, -0.055 um Y Angle = 3.00, increment = 8.00, shift = -17.2 -8.1 pixels, -0.041 um Y Rough eucentricity: changing Z by 0.30 to -215.51, finished. Angle = -4.40, increment = 0.60, shift = 19.9 0.2 pixels, 0.045 um Y Angle = -3.20, increment = 1.80, shift = 57.8 6.6 pixels, 0.133 um Y Angle = 0.41, increment = 5.41, shift = 156.9 18.3 pixels, 0.361 um Y Angle = 3.00, increment = 8.00, shift = 253.1 31.9 pixels, 0.582 um Y Rough eucentricity: changing Z by -4.18 to -219.68, finished. Angle = -4.40, increment = 0.60, shift = 18.9 0.8 pixels, 0.043 um Y Angle = -3.20, increment = 1.80, shift = 54.4 6.9 pixels, 0.125 um Y Angle = 0.41, increment = 5.41, shift = 144.6 16.0 pixels, 0.332 um Y Angle = 3.00, increment = 8.00, shift = 227.7 25.1 pixels, 0.523 um Y Rough eucentricity: changing Z by -3.75 to -223.42, finished. Measured defocus = -3.10 microns changed by -0.90 to target drift = 0.17 nm/sec Trimming A by 23 24 20 145, reference by 26 24 20 120 Backlash adjustment to stage coordinates is 0.36, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 1, label 1 Measured defocus = -3.61 microns changed by -0.39 to target drift = 0.23 nm/sec Trimming A by 26 20 20 145, reference by 21 24 20 140 Backlash adjustment to stage coordinates is 0.12, 0.04 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 3, label 3 Measured defocus = -2.67 microns changed by -1.33 to target drift = 0.13 nm/sec Trimming A by 30 20 20 20, reference by 20 20 20 20 Backlash adjustment to stage coordinates is 0.04, 0.21 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 5, label 5 Trimming A by 22 22 20 145, reference by 21 22 20 135 Backlash adjustment to stage coordinates is 0.20, -0.10 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 7, label 7 Measured defocus = -5.69 microns changed by 1.69 to target drift = 0.20 nm/sec Trimming A by 21 51 140 20, reference by 21 56 140 20 Backlash adjustment to stage coordinates is 0.03, 0.04 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 9, label 9 Drawn line is 149.2 pixels, 341 nm at 138.8 degrees Trimming A by 20 40 110 20, reference by 21 37 125 20 Backlash adjustment to stage coordinates is 0.27, 0.06 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 11, label 11 Drawn line is 724.3 pixels, 1.654 um at 20.7 degrees Measured defocus = 0.39 microns changed by -4.39 to target drift = 0.15 nm/sec Measured defocus = -4.57 microns changed by 0.57 to target drift = 0.12 nm/sec Backlash adjustment to stage coordinates is 0.36, -0.01 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 13, label 13 Backlash adjustment to stage coordinates is 0.28, -0.03 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 15, label 15 Backlash adjustment to stage coordinates is 0.26, -0.09 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 17, label 17 Trimming A by 22 22 20 160, reference by 21 21 20 140 Backlash adjustment to stage coordinates is 0.38, -0.01 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 19, label 19 Drawn line is 531.2 pixels, 1.213 um at 9.7 degrees Measured defocus = -11.53 microns changed by 7.53 to target drift = 0.21 nm/sec Measured defocus = -1.81 microns changed by -2.19 to target drift = 0.06 nm/sec Trimming A by 64 20 20 75, reference by 43 20 20 65 Backlash adjustment to stage coordinates is 0.24, 0.11 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 21, label 21 Measured defocus = -4.73 microns changed by 0.73 to target drift = 0.10 nm/sec Trimming A by 21 20 85 20, reference by 21 20 85 20 Backlash adjustment to stage coordinates is 0.02, 0.12 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 23, label 23 Measured defocus = -3.45 microns changed by -0.55 to target drift = 0.12 nm/sec Trimming A by 21 20 30 115, reference by 24 20 35 110 Backlash adjustment to stage coordinates is 0.16, -0.01 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 25, label 25 Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.25 nm/sec Trimming A by 20 20 20 20, reference by 21 20 25 20 Backlash adjustment to stage coordinates is 0.24, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Stored imaging state in a new parameter set for item # 27, label 27 Eucentric focus value of 0.00000 saved for mag 42000x nanoprobe Focus area changed from stored state, axis position -2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_01.st Running script 1 at item 1 (-388.19, -255.87) 16:08:17 Refine ZLP: additional offset = -4.9 eV; total offset = -8.9 eV Item 1: Stage: -388.19 -255.87 -223 Label: 1 Note: Sec 0 - anchor_maps.map Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Target defocus is: -4.00 um Target defocus is: -4.25 um Target defocus = -4.25 Trimming A by 24 110, reference by 24 110 Angle = -4.40, increment = 0.60, shift = -19.0 -7.0 pixels, -0.045 um Y Trimming A by 23 110, reference by 24 110 Angle = -3.20, increment = 1.80, shift = -67.8 -13.8 pixels, -0.157 um Y Trimming A by 23 110, reference by 24 110 Angle = 0.41, increment = 5.41, shift = -256.6 -39.7 pixels, -0.592 um Y Trimming A by 22 110, reference by 24 110 Angle = 3.00, increment = 8.00, shift = -354.6 -54.5 pixels, -0.818 um Y Rough eucentricity: changing Z by 5.87 to -217.55, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 23 115, reference by 23 115 Angle = 18.00, shift = -64.2, 0.4 pixels, 0.017, -0.146 um, IS = -0.017, 0.146 Trimming A by 23 115, reference by 23 115 Angle = 11.00, shift = -59.8, -2.2 pixels, 0.010, -0.136 um, IS = -0.028, 0.282 Trimming A by 23 115, reference by 23 115 Angle = 4.00, shift = -7.4, 6.2 pixels, 0.016, -0.015 um, IS = -0.043, 0.297 Trimming A by 20 22 20 115, reference by 20 22 20 115 Angle = -4.00, shift = -8.9, -12.2 pixels, -0.025, -0.023 um, IS = -0.018, 0.320 Trimming A by 20 115, reference by 20 115 Angle = -11.00, shift = 21.4, -10.6 pixels, -0.029, 0.046 um, IS = 0.011, 0.275 Trimming A by 22 120, reference by 20 115 Angle = -18.00, shift = -33.6, 4.1 pixels, 0.018, -0.075 um, IS = -0.006, 0.350 Trimming A by 23 115, reference by 23 115 Angle = -24.00, shift = -12.0, 3.5 pixels, 0.011, -0.026 um, IS = -0.017, 0.376 General solution delZ 0.36, delY 1.07, yZero 0.31 solution with assumed YZero 0.31: delZ 0.36, delY 1.06 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.36 microns and lateral displacement of -1.07 microns Trimming A by 20 20 20 120, reference by 26 24 20 120 Disparity in stage position after aligning to center of map frame: -0.09 -1.38 Trimming A by 21 21 20 125, reference by 26 24 20 120 Disparity in stage position after aligning to target position: 0.32 -1.81 (2nd move error 0.03 0.12) Disparity in stage position after aligning to map item itself: -0.03 -1.25 (align shift 0.02 0.00) Finished aligning to item in 3 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -388.26 -257.24 -217.89 0.1 -0.2 unbinned pixels; 0.1 0.0 nm along two shift axes; 0.1 0.0 nm on specimen axes Rate = 0.32898 A/sec Drift is low enough after shot 1 Measured defocus = 2.42 microns changed by -6.67 to target drift = 0.05 nm/sec Measured defocus = -4.90 microns changed by 0.65 to target drift = 0.04 nm/sec Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.02 nm/sec Defocus = -2.131 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.17.26_batch_tomo_01_1_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 139.9 In SetExposureForMean 120, exposure time changed to 1.0800 Image shift 0.092 -0.089 IS units 332.4 -164.2 unbinned pixels; need stage 0.046 0.125 if reset Tilting to -8 Correcting tilt backlash 10.6 -0.5 unbinned pixels; 1.4 3.4 nm along two shift axes; -0.1 3.7 nm on specimen axes Rate = 18.6074 A/sec 6.2 -0.3 unbinned pixels; 0.8 2.0 nm along two shift axes; -0.1 2.2 nm on specimen axes Rate = 10.91609 A/sec Drift is low enough after shot 2 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.09 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -2.176 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.18.30_batch_tomo_01_1_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 126.0 In SetExposureForMean 120, change by a factor of 0.9526 would require too small a change in exposure time Image shift 0.060 -0.104 IS units 340.2 -63.5 unbinned pixels; need stage 0.011 0.124 if reset Tilting to -12 -12.00 degrees -9.6 1.0 unbinned pixels; 1.5 3.1 nm along two shift axes; -0.1 -3.4 nm on specimen axes Rate = 17.01618 A/sec -5.7 2.0 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 10.65024 A/sec Drift is low enough after shot 2 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.07 nm/sec Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.09 nm/sec Defocus = -2.038 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.19.32_batch_tomo_01_1_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 126.0 In SetExposureForMean 120, change by a factor of 0.9524 would require too small a change in exposure time Image shift 0.005 -0.010 IS units 31.0 -5.2 unbinned pixels; need stage 0.001 0.011 if reset Tilting to -14 8.4 -0.2 unbinned pixels; 1.0 2.8 nm along two shift axes; -0.2 3.0 nm on specimen axes Rate = 14.81024 A/sec 6.2 0.7 unbinned pixels; 0.5 2.1 nm along two shift axes; -0.4 2.2 nm on specimen axes Rate = 11.01245 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.11 nm/sec Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.10 nm/sec Defocus = -1.941 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.20.32_batch_tomo_01_1_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 126.0 In SetExposureForMean 120, change by a factor of 0.9522 would require too small a change in exposure time Image shift -0.033 0.071 IS units -225.7 23.0 unbinned pixels; need stage -0.001 -0.083 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 10.9 -7.3 unbinned pixels; 3.7 2.8 nm along two shift axes; 2.2 4.1 nm on specimen axes Rate = 23.03573 A/sec 8.0 0.7 unbinned pixels; 0.7 2.7 nm along two shift axes; -0.5 2.8 nm on specimen axes Rate = 14.13672 A/sec 3.4 -0.5 unbinned pixels; 0.6 1.1 nm along two shift axes; 0.1 1.2 nm on specimen axes Rate = 5.95904 A/sec Drift is low enough after shot 3 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.06 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -2.215 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.21.42_batch_tomo_01_1_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 126.0 In SetExposureForMean 120, change by a factor of 0.9522 would require too small a change in exposure time Image shift 0.101 -0.176 IS units 577.3 -106.4 unbinned pixels; need stage 0.019 0.209 if reset Tilting to -4 Correcting tilt backlash -4.7 0.3 unbinned pixels; 0.7 1.5 nm along two shift axes; 0.0 -1.7 nm on specimen axes Rate = 8.31946 A/sec Drift is low enough after shot 1 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.12 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -2.283 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.22.40_batch_tomo_01_1_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 125.6 In SetExposureForMean 120, change by a factor of 0.9558 would require too small a change in exposure time Image shift 0.111 -0.180 IS units 596.2 -132.2 unbinned pixels; need stage 0.027 0.216 if reset Tilting to -16 -16.00 degrees -3.6 0.3 unbinned pixels; 0.5 1.1 nm along two shift axes; 0.0 -1.3 nm on specimen axes Rate = 6.27352 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.848 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.23.42_batch_tomo_01_1_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 125.9 In SetExposureForMean 120, change by a factor of 0.9530 would require too small a change in exposure time Image shift -0.045 0.088 IS units -281.8 37.9 unbinned pixels; need stage -0.004 -0.105 if reset Tilting to -18 14.9 -6.8 unbinned pixels; 4.0 4.1 nm along two shift axes; 1.9 5.4 nm on specimen axes Rate = 28.67866 A/sec 10.9 0.9 unbinned pixels; 1.0 3.7 nm along two shift axes; -0.7 3.8 nm on specimen axes Rate = 19.26002 A/sec 7.0 -0.3 unbinned pixels; 0.9 2.3 nm along two shift axes; -0.1 2.5 nm on specimen axes Rate = 12.28944 A/sec Drift is low enough after shot 3 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.15 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.15 nm/sec Defocus = -1.866 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.24.49_batch_tomo_01_1_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 125.8 In SetExposureForMean 120, change by a factor of 0.9544 would require too small a change in exposure time Image shift -0.010 0.032 IS units -96.5 -3.3 unbinned pixels; need stage 0.004 -0.036 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 13.1 0.3 unbinned pixels; 1.4 4.4 nm along two shift axes; -0.5 4.6 nm on specimen axes Rate = 23.06738 A/sec 6.6 -0.1 unbinned pixels; 0.8 2.2 nm along two shift axes; -0.2 2.3 nm on specimen axes Rate = 11.53005 A/sec Drift is low enough after shot 2 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.06 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.345 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.25.54_batch_tomo_01_1_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 125.2 In SetExposureForMean 120, change by a factor of 0.9584 would require too small a change in exposure time Image shift 0.117 -0.195 IS units 642.6 -133.2 unbinned pixels; need stage 0.026 0.232 if reset Tilting to 0 Correcting tilt backlash -13.4 3.2 unbinned pixels; 2.6 4.1 nm along two shift axes; -0.7 -4.8 nm on specimen axes Rate = 24.17179 A/sec -6.7 2.6 unbinned pixels; 1.6 1.9 nm along two shift axes; -0.7 -2.4 nm on specimen axes Rate = 12.52323 A/sec Drift is low enough after shot 2 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.21 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.16 nm/sec Defocus = -2.437 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.26.57_batch_tomo_01_1_009_0.0.tif Saved Z = 9, 0.00 degrees Mean of buffer A = 125.1 In SetExposureForMean 120, change by a factor of 0.9593 would require too small a change in exposure time Image shift 0.091 -0.130 IS units 442.6 -123.6 unbinned pixels; need stage 0.029 0.160 if reset Tilting to -20 -20.00 degrees -0.8 0.9 unbinned pixels; 0.4 0.2 nm along two shift axes; -0.3 -0.3 nm on specimen axes Rate = 2.04783 A/sec Drift is low enough after shot 1 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.05 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.01 nm/sec Defocus = -1.769 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.27.55_batch_tomo_01_1_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 125.5 In SetExposureForMean 120, change by a factor of 0.9568 would require too small a change in exposure time Image shift -0.052 0.099 IS units -320.1 45.6 unbinned pixels; need stage -0.006 -0.122 if reset Tilting to -22 12.6 -6.4 unbinned pixels; 3.6 3.4 nm along two shift axes; 1.9 4.6 nm on specimen axes Rate = 24.71338 A/sec 10.5 1.4 unbinned pixels; 0.7 3.6 nm along two shift axes; -0.8 3.6 nm on specimen axes Rate = 18.59585 A/sec 4.7 0.3 unbinned pixels; 0.4 1.6 nm along two shift axes; -0.3 1.6 nm on specimen axes Rate = 8.30275 A/sec Drift is low enough after shot 3 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.15 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.706 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.29.02_batch_tomo_01_1_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 124.7 In SetExposureForMean 120, change by a factor of 0.9628 would require too small a change in exposure time Image shift -0.019 0.055 IS units -167.8 0.5 unbinned pixels; need stage 0.005 -0.064 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 8.5 -0.7 unbinned pixels; 1.2 2.7 nm along two shift axes; -0.0 3.0 nm on specimen axes Rate = 14.96974 A/sec 6.4 0.6 unbinned pixels; 0.5 2.2 nm along two shift axes; -0.4 2.2 nm on specimen axes Rate = 11.30486 A/sec Drift is low enough after shot 2 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.05 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.517 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.30.07_batch_tomo_01_1_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 124.7 In SetExposureForMean 120, change by a factor of 0.9625 would require too small a change in exposure time Image shift 0.104 -0.174 IS units 573.3 -119.3 unbinned pixels; need stage 0.023 0.207 if reset Tilting to 4 Correcting tilt backlash -33.8 7.8 unbinned pixels; 6.5 10.3 nm along two shift axes; -1.7 -12.1 nm on specimen axes Rate = 60.79438 A/sec -24.6 6.9 unbinned pixels; 5.2 7.3 nm along two shift axes; -1.7 -8.9 nm on specimen axes Rate = 44.8457 A/sec -18.1 4.9 unbinned pixels; 3.7 5.4 nm along two shift axes; -1.2 -6.5 nm on specimen axes Rate = 32.78392 A/sec -14.0 3.6 unbinned pixels; 2.8 4.2 nm along two shift axes; -0.8 -5.0 nm on specimen axes Rate = 25.41592 A/sec -8.0 0.8 unbinned pixels; 1.2 2.5 nm along two shift axes; -0.0 -2.8 nm on specimen axes Rate = 14.08348 A/sec -8.5 3.7 unbinned pixels; 2.2 2.4 nm along two shift axes; -1.0 -3.1 nm on specimen axes Rate = 16.26983 A/sec -8.0 2.0 unbinned pixels; 1.6 2.4 nm along two shift axes; -0.4 -2.9 nm on specimen axes Rate = 14.5053 A/sec -6.5 2.1 unbinned pixels; 1.5 1.9 nm along two shift axes; -0.5 -2.4 nm on specimen axes Rate = 12.02776 A/sec Drift is low enough after shot 8 Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.19 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.17 nm/sec Defocus = -2.582 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.31.43_batch_tomo_01_1_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 124.1 In SetExposureForMean 120, change by a factor of 0.9670 would require too small a change in exposure time Image shift 0.071 -0.106 IS units 358.1 -91.8 unbinned pixels; need stage 0.021 0.130 if reset Tilting to -24 -24.00 degrees -2.8 1.8 unbinned pixels; 0.9 0.7 nm along two shift axes; -0.5 -1.0 nm on specimen axes Rate = 5.77188 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.04 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = -1.603 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.32.47_batch_tomo_01_1_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 124.4 In SetExposureForMean 120, change by a factor of 0.9652 would require too small a change in exposure time Image shift -0.048 0.091 IS units -294.0 43.2 unbinned pixels; need stage -0.006 -0.115 if reset Tilting to -26 15.9 -5.9 unbinned pixels; 3.8 4.6 nm along two shift axes; 1.6 5.8 nm on specimen axes Rate = 29.78676 A/sec 10.5 1.6 unbinned pixels; 0.7 3.7 nm along two shift axes; -0.9 3.6 nm on specimen axes Rate = 18.61411 A/sec 5.8 0.2 unbinned pixels; 0.6 1.9 nm along two shift axes; -0.2 2.0 nm on specimen axes Rate = 10.15159 A/sec Drift is low enough after shot 3 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.11 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.14 nm/sec Defocus = -1.528 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.33.59_batch_tomo_01_1_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 123.6 In SetExposureForMean 120, change by a factor of 0.9712 would require too small a change in exposure time Image shift -0.020 0.049 IS units -151.8 6.5 unbinned pixels; need stage 0.002 -0.060 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 19.9 -0.1 unbinned pixels; 2.4 6.6 nm along two shift axes; -0.6 7.0 nm on specimen axes Rate = 34.85341 A/sec 1.3 1.8 unbinned pixels; 0.4 0.6 nm along two shift axes; -0.7 0.4 nm on specimen axes Rate = 3.88105 A/sec Drift is low enough after shot 2 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -2.641 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.35.11_batch_tomo_01_1_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 123.6 In SetExposureForMean 120, change by a factor of 0.9708 would require too small a change in exposure time Image shift 0.096 -0.177 IS units 575.0 -92.6 unbinned pixels; need stage 0.014 0.207 if reset Tilting to 8 Correcting tilt backlash -38.0 8.8 unbinned pixels; 7.4 11.5 nm along two shift axes; -1.9 -13.6 nm on specimen axes Rate = 68.29324 A/sec -11.6 2.8 unbinned pixels; 2.3 3.5 nm along two shift axes; -0.6 -4.2 nm on specimen axes Rate = 20.94742 A/sec -9.3 4.4 unbinned pixels; 2.5 2.5 nm along two shift axes; -1.3 -3.4 nm on specimen axes Rate = 18.0199 A/sec -4.3 1.5 unbinned pixels; 1.0 1.2 nm along two shift axes; -0.4 -1.5 nm on specimen axes Rate = 7.90096 A/sec Drift is low enough after shot 4 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.10 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -2.727 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.36.25_batch_tomo_01_1_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 123.0 In SetExposureForMean 120, change by a factor of 0.9757 would require too small a change in exposure time Image shift 0.062 -0.092 IS units 311.4 -81.1 unbinned pixels; need stage 0.018 0.114 if reset Tilting to -28 -28.00 degrees 0.3 0.2 unbinned pixels; 0.0 0.1 nm along two shift axes; -0.1 0.1 nm on specimen axes Rate = 0.55604 A/sec Drift is low enough after shot 1 Measured defocus = -4.36 microns changed by 0.11 to target drift = 0.06 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.12 nm/sec Defocus = -1.425 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.37.24_batch_tomo_01_1_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 123.1 In SetExposureForMean 120, change by a factor of 0.9758 would require too small a change in exposure time Image shift -0.024 0.034 IS units -115.5 33.9 unbinned pixels; need stage -0.008 -0.047 if reset Tilting to -30 11.8 -7.4 unbinned pixels; 3.8 3.0 nm along two shift axes; 2.2 4.3 nm on specimen axes Rate = 24.30518 A/sec 8.4 2.0 unbinned pixels; 0.3 3.0 nm along two shift axes; -1.0 2.9 nm on specimen axes Rate = 15.10808 A/sec 4.8 0.6 unbinned pixels; 0.4 1.7 nm along two shift axes; -0.4 1.7 nm on specimen axes Rate = 8.50202 A/sec Drift is low enough after shot 3 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.04 nm/sec Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.09 nm/sec Defocus = -1.431 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.38.30_batch_tomo_01_1_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 122.6 In SetExposureForMean 120, change by a factor of 0.9794 would require too small a change in exposure time Image shift 0.015 -0.036 IS units 113.0 -6.3 unbinned pixels; need stage -0.001 0.046 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -9.0 0.1 unbinned pixels; 1.1 3.0 nm along two shift axes; 0.3 -3.2 nm on specimen axes Rate = 15.86825 A/sec 0.1 4.0 unbinned pixels; 1.3 0.5 nm along two shift axes; -1.4 -0.1 nm on specimen axes Rate = 6.98667 A/sec Drift is low enough after shot 2 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.09 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -2.799 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.39.37_batch_tomo_01_1_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 122.1 In SetExposureForMean 120, change by a factor of 0.9827 would require too small a change in exposure time Image shift 0.086 -0.178 IS units 566.1 -66.4 unbinned pixels; need stage 0.005 0.205 if reset Tilting to 12 Correcting tilt backlash -8.3 2.6 unbinned pixels; 1.8 2.4 nm along two shift axes; -0.7 -3.0 nm on specimen axes Rate = 15.24431 A/sec -2.3 1.3 unbinned pixels; 0.7 0.6 nm along two shift axes; -0.4 -0.8 nm on specimen axes Rate = 4.51906 A/sec Drift is low enough after shot 2 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -2.853 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.40.41_batch_tomo_01_1_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 121.4 In SetExposureForMean 120, change by a factor of 0.9887 would require too small a change in exposure time Image shift 0.086 -0.156 IS units 507.6 -86.1 unbinned pixels; need stage 0.014 0.186 if reset Tilting to -32 -32.00 degrees 4.9 -4.2 unbinned pixels; 2.0 1.1 nm along two shift axes; 1.3 1.9 nm on specimen axes Rate = 11.31249 A/sec Drift is low enough after shot 1 Measured defocus = -4.38 microns changed by 0.13 to target drift = 0.14 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.14 nm/sec Defocus = -1.310 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.41.41_batch_tomo_01_1_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 121.8 In SetExposureForMean 120, change by a factor of 0.9864 would require too small a change in exposure time Image shift 0.034 -0.102 IS units 310.4 4.9 unbinned pixels; need stage -0.011 0.129 if reset Tilting to -34 13.3 -6.7 unbinned pixels; 3.8 3.6 nm along two shift axes; 2.0 4.9 nm on specimen axes Rate = 26.07563 A/sec 7.4 2.4 unbinned pixels; 0.1 2.7 nm along two shift axes; -1.1 2.5 nm on specimen axes Rate = 13.66743 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.04 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.19 nm/sec Defocus = -1.283 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.42.44_batch_tomo_01_1_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 120.9 In SetExposureForMean 120, change by a factor of 0.9937 would require too small a change in exposure time Image shift 0.068 -0.166 IS units 517.5 -27.6 unbinned pixels; need stage -0.006 0.222 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -6.7 4.4 unbinned pixels; 2.2 1.7 nm along two shift axes; -1.3 -2.5 nm on specimen axes Rate = 14.01412 A/sec -1.2 1.4 unbinned pixels; 0.6 0.2 nm along two shift axes; -0.5 -0.5 nm on specimen axes Rate = 3.26788 A/sec Drift is low enough after shot 2 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.10 nm/sec Defocus = -2.866 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.43.51_batch_tomo_01_1_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 120.7 In SetExposureForMean 120, change by a factor of 0.9947 would require too small a change in exposure time Image shift 0.082 -0.163 IS units 522.0 -67.4 unbinned pixels; need stage 0.007 0.192 if reset Tilting to 16 Correcting tilt backlash -7.4 -3.3 unbinned pixels; 0.2 2.9 nm along two shift axes; 1.4 -2.5 nm on specimen axes Rate = 14.34506 A/sec -0.7 3.2 unbinned pixels; 1.1 0.1 nm along two shift axes; -1.1 -0.4 nm on specimen axes Rate = 5.71155 A/sec Drift is low enough after shot 2 Measured defocus = -4.20 microns changed by -0.05 to target drift = 0.22 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.14 nm/sec Defocus = -2.918 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.44.54_batch_tomo_01_1_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 119.6 In SetExposureForMean 120, change by a factor of 1.0037 would require too small a change in exposure time Image shift 0.121 -0.230 IS units 742.0 -109.6 unbinned pixels; need stage 0.015 0.277 if reset Tilting to -36 -36.00 degrees 19.4 -3.6 unbinned pixels; 3.4 6.0 nm along two shift axes; 0.7 6.9 nm on specimen axes Rate = 34.63169 A/sec 1.2 1.8 unbinned pixels; 0.5 0.6 nm along two shift axes; -0.7 0.4 nm on specimen axes Rate = 3.85246 A/sec Drift is low enough after shot 2 Measured defocus = -4.37 microns changed by 0.12 to target drift = 0.13 nm/sec Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.13 nm/sec Defocus = -1.198 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.46.05_batch_tomo_01_1_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 120.1 In SetExposureForMean 120, change by a factor of 1.0001 would require too small a change in exposure time Image shift 0.096 -0.245 IS units 759.6 -28.3 unbinned pixels; need stage -0.014 0.333 if reset Tilting to -38 13.3 -1.4 unbinned pixels; 2.0 4.2 nm along two shift axes; 0.1 4.7 nm on specimen axes Rate = 23.48549 A/sec 11.9 0.2 unbinned pixels; 1.3 4.0 nm along two shift axes; -0.4 4.2 nm on specimen axes Rate = 20.89285 A/sec 4.4 0.0 unbinned pixels; 0.5 1.5 nm along two shift axes; -0.1 1.5 nm on specimen axes Rate = 7.69799 A/sec Drift is low enough after shot 3 Measured defocus = -4.44 microns changed by 0.19 to target drift = 0.15 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.11 nm/sec Defocus = -1.013 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.47.16_batch_tomo_01_1_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 119.1 In SetExposureForMean 120, change by a factor of 1.0079 would require too small a change in exposure time Image shift 0.110 -0.274 IS units 850.5 -39.9 unbinned pixels; need stage -0.012 0.383 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -4.5 3.1 unbinned pixels; 1.5 1.1 nm along two shift axes; -0.9 -1.7 nm on specimen axes Rate = 9.49962 A/sec Drift is low enough after shot 1 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.21 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.10 nm/sec Defocus = -2.985 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.48.18_batch_tomo_01_1_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 118.7 In SetExposureForMean 120, change by a factor of 1.0112 would require too small a change in exposure time Image shift 0.116 -0.245 IS units 778.2 -83.4 unbinned pixels; need stage 0.005 0.292 if reset Tilting to 20 Correcting tilt backlash -8.1 -1.4 unbinned pixels; 0.5 2.9 nm along two shift axes; 0.8 -2.8 nm on specimen axes Rate = 14.4739 A/sec -4.7 5.9 unbinned pixels; 2.5 0.8 nm along two shift axes; -1.9 -1.8 nm on specimen axes Rate = 13.30569 A/sec Drift is low enough after shot 2 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.16 nm/sec Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.08 nm/sec Defocus = -3.190 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.49.21_batch_tomo_01_1_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0226 would require too small a change in exposure time Image shift 0.164 -0.358 IS units 1130.8 -106.4 unbinned pixels; need stage 0.002 0.429 if reset Tilting to -40 -40.00 degrees 21.0 -7.1 unbinned pixels; 4.8 6.1 nm along two shift axes; 1.8 7.6 nm on specimen axes Rate = 38.87157 A/sec 4.5 2.2 unbinned pixels; 0.2 1.8 nm along two shift axes; -0.9 1.5 nm on specimen axes Rate = 8.82872 A/sec Drift is low enough after shot 2 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.30 nm/sec Measured defocus = -4.20 microns changed by -0.05 to target drift = 0.20 nm/sec Defocus = -0.988 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.50.27_batch_tomo_01_1_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 118.0 In SetExposureForMean 120, change by a factor of 1.0172 would require too small a change in exposure time Image shift 0.163 -0.408 IS units 1265.2 -55.8 unbinned pixels; need stage -0.020 0.586 if reset Tilting to -42 11.3 -1.5 unbinned pixels; 1.8 3.6 nm along two shift axes; 0.2 4.0 nm on specimen axes Rate = 20.01367 A/sec 7.8 0.7 unbinned pixels; 0.7 2.7 nm along two shift axes; -0.5 2.7 nm on specimen axes Rate = 13.71272 A/sec Drift is low enough after shot 2 Measured defocus = -4.43 microns changed by 0.18 to target drift = 0.32 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.16 nm/sec Defocus = -0.834 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.51.29_batch_tomo_01_1_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 116.5 In SetExposureForMean 120, change by a factor of 1.0303 would require too small a change in exposure time Image shift 0.177 -0.435 IS units 1353.1 -66.3 unbinned pixels; need stage -0.019 0.647 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -9.6 1.9 unbinned pixels; 1.8 2.9 nm along two shift axes; -0.4 -3.4 nm on specimen axes Rate = 17.17062 A/sec -11.0 2.2 unbinned pixels; 2.0 3.4 nm along two shift axes; -0.4 -3.9 nm on specimen axes Rate = 19.62359 A/sec -6.3 2.6 unbinned pixels; 1.6 1.8 nm along two shift axes; -0.7 -2.3 nm on specimen axes Rate = 12.01763 A/sec Drift is low enough after shot 3 Measured defocus = -4.38 microns changed by 0.13 to target drift = 0.29 nm/sec Measured defocus = -4.07 microns changed by -0.18 to target drift = 0.08 nm/sec Defocus = -3.237 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.52.47_batch_tomo_01_1_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 116.4 In SetExposureForMean 120, change by a factor of 1.0310 would require too small a change in exposure time Image shift 0.158 -0.356 IS units 1121.6 -91.4 unbinned pixels; need stage -0.003 0.431 if reset Tilting to 24 Correcting tilt backlash -6.3 -1.4 unbinned pixels; 0.3 2.3 nm along two shift axes; 0.7 -2.2 nm on specimen axes Rate = 11.34616 A/sec Drift is low enough after shot 1 Measured defocus = -4.35 microns changed by 0.10 to target drift = 0.10 nm/sec Measured defocus = -4.42 microns changed by 0.17 to target drift = 0.28 nm/sec Defocus = -2.971 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.53.45_batch_tomo_01_1_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 115.0 In SetExposureForMean 120, change by a factor of 1.0437 would require too small a change in exposure time Image shift 0.203 -0.469 IS units 1471.2 -104.8 unbinned pixels; need stage -0.009 0.573 if reset Tilting to -44 -44.00 degrees 14.7 -6.7 unbinned pixels; 3.9 4.1 nm along two shift axes; 1.9 5.4 nm on specimen axes Rate = 28.29941 A/sec 0.5 2.7 unbinned pixels; 0.9 0.5 nm along two shift axes; -1.0 0.1 nm on specimen axes Rate = 4.89773 A/sec Drift is low enough after shot 2 Measured defocus = -4.12 microns changed by -0.13 to target drift = 0.27 nm/sec Measured defocus = -4.39 microns changed by 0.14 to target drift = 0.16 nm/sec Defocus = -0.830 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.54.51_batch_tomo_01_1_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 115.6 In SetExposureForMean 120, change by a factor of 1.0384 would require too small a change in exposure time Image shift 0.229 -0.557 IS units 1733.6 -93.3 unbinned pixels; need stage -0.021 0.856 if reset Tilting to -46 -7.5 -1.8 unbinned pixels; 0.3 2.7 nm along two shift axes; 0.8 -2.6 nm on specimen axes Rate = 13.6253 A/sec Drift is low enough after shot 1 Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.13 nm/sec Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.08 nm/sec Defocus = -0.760 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.55.46_batch_tomo_01_1_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 114.1 In SetExposureForMean 120, change by a factor of 1.0522 would require too small a change in exposure time Image shift 0.244 -0.574 IS units 1795.5 -117.8 unbinned pixels; need stage -0.015 0.919 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -1.0 -0.9 unbinned pixels; 0.2 0.5 nm along two shift axes; 0.4 -0.3 nm on specimen axes Rate = 2.46641 A/sec Drift is low enough after shot 1 Measured defocus = -4.38 microns changed by 0.13 to target drift = 0.13 nm/sec Measured defocus = -4.07 microns changed by -0.18 to target drift = 0.51 nm/sec Defocus = -3.022 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.56.49_batch_tomo_01_1_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 113.6 In SetExposureForMean 120, exposure time changed to 1.2000 WARNING: Desired exposure time (1.141) differs from actual one (1.200) by more than 5% Image shift 0.209 -0.461 IS units 1456.0 -131.0 unbinned pixels; need stage 0.000 0.578 if reset Tilting to 28 Correcting tilt backlash 10.3 -5.3 unbinned pixels; 3.0 2.8 nm along two shift axes; 1.5 3.8 nm on specimen axes Rate = 20.33923 A/sec 2.5 2.6 unbinned pixels; 0.6 1.2 nm along two shift axes; -1.0 0.8 nm on specimen axes Rate = 6.42007 A/sec Drift is low enough after shot 2 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.22 nm/sec Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.81 nm/sec Defocus = -3.187 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.57.53_batch_tomo_01_1_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 124.0 In SetExposureForMean 120, change by a factor of 0.9686 would require too small a change in exposure time Image shift 0.254 -0.587 IS units 1841.3 -131.1 unbinned pixels; need stage -0.012 0.742 if reset Tilting to -48 -48.00 degrees -1.3 -5.2 unbinned pixels; 1.6 1.0 nm along two shift axes; 1.9 -0.3 nm on specimen axes Rate = 9.43286 A/sec Drift is low enough after shot 1 Measured defocus = -4.39 microns changed by 0.14 to target drift = 0.43 nm/sec Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.60 nm/sec Defocus = -0.584 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_16.58.55_batch_tomo_01_1_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 124.7 In SetExposureForMean 120, change by a factor of 0.9632 would require too small a change in exposure time Image shift 0.284 -0.665 IS units 2081.9 -141.5 unbinned pixels; need stage -0.015 1.107 if reset Tilting to -50 -15.1 -0.4 unbinned pixels; 1.6 5.0 nm along two shift axes; 0.6 -5.3 nm on specimen axes Rate = 26.50833 A/sec 3.8 0.1 unbinned pixels; 0.4 1.3 nm along two shift axes; -0.2 1.3 nm on specimen axes Rate = 6.59451 A/sec Drift is low enough after shot 2 Measured defocus = -3.97 microns changed by -0.28 to target drift = 0.94 nm/sec Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.09 nm/sec Defocus = -0.799 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.00.02_batch_tomo_01_1_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 122.4 In SetExposureForMean 120, change by a factor of 0.9815 would require too small a change in exposure time Image shift 0.299 -0.681 IS units 2139.9 -166.5 unbinned pixels; need stage -0.009 1.186 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -10.1 2.1 unbinned pixels; 1.9 3.1 nm along two shift axes; -0.4 -3.6 nm on specimen axes Rate = 18.02564 A/sec -16.0 4.9 unbinned pixels; 3.5 4.7 nm along two shift axes; -1.2 -5.8 nm on specimen axes Rate = 29.31863 A/sec -5.9 2.5 unbinned pixels; 1.5 1.6 nm along two shift axes; -0.7 -2.1 nm on specimen axes Rate = 11.24282 A/sec Drift is low enough after shot 3 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.33 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -3.127 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.01.15_batch_tomo_01_1_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 121.7 In SetExposureForMean 120, change by a factor of 0.9873 would require too small a change in exposure time Image shift 0.243 -0.536 IS units 1692.2 -151.2 unbinned pixels; need stage -0.000 0.697 if reset Tilting to 32 Correcting tilt backlash -6.5 -6.0 unbinned pixels; 1.2 2.9 nm along two shift axes; 2.3 -2.1 nm on specimen axes Rate = 15.69484 A/sec -7.1 2.6 unbinned pixels; 1.7 2.0 nm along two shift axes; -0.7 -2.6 nm on specimen axes Rate = 13.32205 A/sec Drift is low enough after shot 2 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.16 nm/sec Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.25 nm/sec Defocus = -3.091 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.02.19_batch_tomo_01_1_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 119.4 In SetExposureForMean 120, change by a factor of 1.0059 would require too small a change in exposure time Image shift 0.288 -0.651 IS units 2047.0 -163.3 unbinned pixels; need stage -0.007 0.860 if reset Tilting to -52 -52.00 degrees 10.8 -6.8 unbinned pixels; 3.5 2.8 nm along two shift axes; 2.1 4.0 nm on specimen axes Rate = 22.46243 A/sec 17.0 1.3 unbinned pixels; 1.5 5.8 nm along two shift axes; -1.0 5.9 nm on specimen axes Rate = 29.86402 A/sec Trimming A by 60 60, reference by 20 20 34.2 -0.1 unbinned pixels; 4.0 11.3 nm along two shift axes; -1.0 12.0 nm on specimen axes Rate = 60.02137 A/sec -21.5 0.4 unbinned pixels; 2.6 7.1 nm along two shift axes; 0.5 -7.6 nm on specimen axes Rate = 37.80105 A/sec 3.1 0.9 unbinned pixels; 0.1 1.1 nm along two shift axes; -0.4 1.1 nm on specimen axes Rate = 5.74526 A/sec Drift is low enough after shot 5 Measured defocus = -4.36 microns changed by 0.11 to target drift = 0.34 nm/sec Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.43 nm/sec Defocus = -0.761 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.03.42_batch_tomo_01_1_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 120.3 In SetExposureForMean 120, change by a factor of 0.9985 would require too small a change in exposure time Image shift 0.339 -0.752 IS units 2372.4 -208.3 unbinned pixels; need stage -0.001 1.373 if reset Tilting to -54 12.0 -0.5 unbinned pixels; 1.6 3.9 nm along two shift axes; -0.2 4.2 nm on specimen axes Rate = 21.00888 A/sec 7.1 1.2 unbinned pixels; 0.4 2.5 nm along two shift axes; -0.6 2.5 nm on specimen axes Rate = 12.73201 A/sec Drift is low enough after shot 2 Measured defocus = -4.39 microns changed by 0.14 to target drift = 0.14 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.22 nm/sec Defocus = -0.643 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.04.44_batch_tomo_01_1_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 117.7 In SetExposureForMean 120, change by a factor of 1.0198 would require too small a change in exposure time Image shift 0.341 -0.747 IS units 2358.7 -218.0 unbinned pixels; need stage 0.002 1.431 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -0.8 1.5 unbinned pixels; 0.6 0.1 nm along two shift axes; -0.5 -0.3 nm on specimen axes Rate = 3.00165 A/sec Drift is low enough after shot 1 Measured defocus = -4.00 microns changed by -0.25 to target drift = 0.48 nm/sec Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.21 nm/sec Defocus = -3.250 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.05.48_batch_tomo_01_1_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 116.9 In SetExposureForMean 120, change by a factor of 1.0281 would require too small a change in exposure time Image shift 0.287 -0.614 IS units 1946.1 -197.0 unbinned pixels; need stage 0.008 0.838 if reset Tilting to 36 Correcting tilt backlash -8.3 -3.9 unbinned pixels; 0.3 3.2 nm along two shift axes; 1.6 -2.8 nm on specimen axes Rate = 16.18405 A/sec -5.1 3.7 unbinned pixels; 1.8 1.2 nm along two shift axes; -1.2 -1.9 nm on specimen axes Rate = 11.07077 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.26 nm/sec Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.14 nm/sec Defocus = -3.164 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.06.51_batch_tomo_01_1_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 114.0 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift 0.326 -0.710 IS units 2244.1 -213.4 unbinned pixels; need stage 0.004 0.989 if reset Tilting to -56 -56.00 degrees 15.4 -5.6 unbinned pixels; 3.7 4.4 nm along two shift axes; 1.5 5.6 nm on specimen axes Rate = 28.6693 A/sec 1.9 2.6 unbinned pixels; 0.6 1.0 nm along two shift axes; -1.0 0.6 nm on specimen axes Rate = 5.76565 A/sec Drift is low enough after shot 2 Measured defocus = -4.41 microns changed by 0.16 to target drift = 0.23 nm/sec Measured defocus = -4.05 microns changed by -0.20 to target drift = 0.67 nm/sec Defocus = -0.689 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.07.59_batch_tomo_01_1_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 126.9 In SetExposureForMean 120, exposure time changed to 1.2000 Image shift 0.376 -0.806 IS units 2553.0 -257.5 unbinned pixels; need stage 0.010 1.629 if reset Tilting to -58 -1.3 -0.6 unbinned pixels; 0.0 0.5 nm along two shift axes; 0.3 -0.4 nm on specimen axes Rate = 2.57493 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.14 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.18 nm/sec Defocus = -0.607 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.08.55_batch_tomo_01_1_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 112.5 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift 0.384 -0.827 IS units 2618.8 -259.2 unbinned pixels; need stage 0.009 1.763 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -9.1 3.4 unbinned pixels; 2.2 2.6 nm along two shift axes; -0.9 -3.3 nm on specimen axes Rate = 16.98392 A/sec -11.2 5.0 unbinned pixels; 3.0 3.1 nm along two shift axes; -1.4 -4.1 nm on specimen axes Rate = 21.51891 A/sec 13.8 -0.5 unbinned pixels; 1.8 4.5 nm along two shift axes; -0.3 4.9 nm on specimen axes Rate = 24.21917 A/sec -14.1 3.6 unbinned pixels; 2.8 4.3 nm along two shift axes; -0.8 -5.1 nm on specimen axes Rate = 25.58426 A/sec -8.8 3.3 unbinned pixels; 2.1 2.5 nm along two shift axes; -0.9 -3.2 nm on specimen axes Rate = 16.52146 A/sec -2.2 1.9 unbinned pixels; 0.9 0.5 nm along two shift axes; -0.6 -0.8 nm on specimen axes Rate = 5.00644 A/sec Drift is low enough after shot 6 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.06 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = -3.263 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.10.26_batch_tomo_01_1_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 122.7 In SetExposureForMean 120, change by a factor of 0.9791 would require too small a change in exposure time Image shift 0.323 -0.692 IS units 2193.7 -221.1 unbinned pixels; need stage 0.009 0.993 if reset Tilting to 40 Correcting tilt backlash -12.0 -4.4 unbinned pixels; 0.0 4.5 nm along two shift axes; 1.9 -4.1 nm on specimen axes Rate = 22.46275 A/sec -1.4 3.1 unbinned pixels; 1.2 0.1 nm along two shift axes; -1.1 -0.6 nm on specimen axes Rate = 6.04998 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.10 nm/sec Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.31 nm/sec Defocus = -3.269 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.11.30_batch_tomo_01_1_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 119.0 In SetExposureForMean 120, change by a factor of 1.0098 would require too small a change in exposure time Image shift 0.357 -0.787 IS units 2483.0 -223.3 unbinned pixels; need stage 0.000 1.156 if reset Tilting to -60 -60.00 degrees 17.6 -5.9 unbinned pixels; 4.0 5.1 nm along two shift axes; 1.5 6.4 nm on specimen axes Rate = 32.55862 A/sec 10.8 4.2 unbinned pixels; 0.1 4.1 nm along two shift axes; -1.8 3.6 nm on specimen axes Rate = 20.32977 A/sec 6.2 0.3 unbinned pixels; 0.6 2.1 nm along two shift axes; -0.3 2.2 nm on specimen axes Rate = 10.90332 A/sec Drift is low enough after shot 3 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.17 nm/sec Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.40 nm/sec Defocus = -0.466 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.12.44_batch_tomo_01_1_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 120.5 In SetExposureForMean 120, change by a factor of 0.9949 would require too small a change in exposure time Image shift 0.413 -0.891 IS units 2820.7 -275.5 unbinned pixels; need stage 0.008 2.013 if reset Tilting to -62 20.8 -0.5 unbinned pixels; 2.6 6.8 nm along two shift axes; -0.4 7.3 nm on specimen axes Rate = 36.5494 A/sec 9.7 1.6 unbinned pixels; 0.6 3.4 nm along two shift axes; -0.9 3.3 nm on specimen axes Rate = 17.19202 A/sec 6.5 0.1 unbinned pixels; 0.7 2.1 nm along two shift axes; -0.2 2.3 nm on specimen axes Rate = 11.33658 A/sec Drift is low enough after shot 3 Measured defocus = -4.10 microns changed by -0.15 to target drift = 0.08 nm/sec Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.28 nm/sec Defocus = -0.550 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.13.56_batch_tomo_01_1_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 116.8 In SetExposureForMean 120, change by a factor of 1.0271 would require too small a change in exposure time Image shift 0.418 -0.909 IS units 2876.0 -272.9 unbinned pixels; need stage 0.005 2.185 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -24.5 4.4 unbinned pixels; 4.3 7.6 nm along two shift axes; -0.8 -8.7 nm on specimen axes Rate = 43.70272 A/sec -7.6 4.7 unbinned pixels; 2.4 2.0 nm along two shift axes; -1.4 -2.8 nm on specimen axes Rate = 15.67297 A/sec -3.6 1.3 unbinned pixels; 0.8 1.0 nm along two shift axes; -0.3 -1.3 nm on specimen axes Rate = 6.67669 A/sec Drift is low enough after shot 3 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.54 nm/sec Measured defocus = -4.10 microns changed by -0.15 to target drift = 0.24 nm/sec Defocus = -3.458 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.15.16_batch_tomo_01_1_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 114.6 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.362 -0.793 IS units 2504.3 -231.0 unbinned pixels; need stage 0.002 1.202 if reset Tilting to 44 Correcting tilt backlash -8.4 -6.2 unbinned pixels; 1.1 3.5 nm along two shift axes; 2.4 -2.7 nm on specimen axes Rate = 18.37227 A/sec -10.3 2.5 unbinned pixels; 2.0 3.1 nm along two shift axes; -0.6 -3.7 nm on specimen axes Rate = 18.54847 A/sec -9.5 2.4 unbinned pixels; 1.9 2.9 nm along two shift axes; -0.6 -3.4 nm on specimen axes Rate = 17.24047 A/sec -9.0 1.6 unbinned pixels; 1.6 2.8 nm along two shift axes; -0.3 -3.2 nm on specimen axes Rate = 15.98238 A/sec -2.6 -0.5 unbinned pixels; 0.1 0.9 nm along two shift axes; 0.3 -0.9 nm on specimen axes Rate = 4.71898 A/sec Drift is low enough after shot 5 Measured defocus = -4.50 microns changed by 0.25 to target drift = 0.62 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.31 nm/sec Defocus = -3.232 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.16.36_batch_tomo_01_1_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 119.4 In SetExposureForMean 120, change by a factor of 1.0072 would require too small a change in exposure time Image shift 0.420 -0.913 IS units 2888.0 -273.6 unbinned pixels; need stage 0.005 1.432 if reset Tilting to -64 -64.00 degrees 16.7 -7.0 unbinned pixels; 4.3 4.7 nm along two shift axes; 1.9 6.1 nm on specimen axes Rate = 31.76217 A/sec 10.2 2.2 unbinned pixels; 0.5 3.7 nm along two shift axes; -1.1 3.5 nm on specimen axes Rate = 18.41841 A/sec 9.3 0.0 unbinned pixels; 1.1 3.1 nm along two shift axes; -0.3 3.3 nm on specimen axes Rate = 16.31941 A/sec 4.2 0.0 unbinned pixels; 0.5 1.4 nm along two shift axes; -0.1 1.5 nm on specimen axes Rate = 7.32941 A/sec Drift is low enough after shot 4 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.19 nm/sec Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.60 nm/sec Defocus = -0.518 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.17.56_batch_tomo_01_1_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 124.0 In SetExposureForMean 120, change by a factor of 0.9660 would require too small a change in exposure time Image shift 0.458 -0.983 IS units 3113.6 -311.2 unbinned pixels; need stage 0.011 2.534 if reset Tilting to -66 14.7 -4.5 unbinned pixels; 3.2 4.3 nm along two shift axes; 1.1 5.3 nm on specimen axes Rate = 26.86968 A/sec 18.7 3.4 unbinned pixels; 1.1 6.6 nm along two shift axes; -1.8 6.5 nm on specimen axes Rate = 33.42218 A/sec 6.7 1.8 unbinned pixels; 0.2 2.4 nm along two shift axes; -0.8 2.3 nm on specimen axes Rate = 12.18404 A/sec Drift is low enough after shot 3 Measured defocus = -4.59 microns changed by 0.34 to target drift = 0.36 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.23 nm/sec Defocus = -0.212 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.19.03_batch_tomo_01_1_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 118.9 In SetExposureForMean 120, change by a factor of 1.0068 would require too small a change in exposure time Image shift 0.477 -1.027 IS units 3251.3 -321.5 unbinned pixels; need stage 0.011 2.852 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -18.5 4.9 unbinned pixels; 3.8 5.5 nm along two shift axes; -1.2 -6.6 nm on specimen axes Rate = 33.49418 A/sec -10.5 2.1 unbinned pixels; 1.9 3.2 nm along two shift axes; -0.4 -3.7 nm on specimen axes Rate = 18.77509 A/sec -12.1 5.7 unbinned pixels; 3.3 3.3 nm along two shift axes; -1.6 -4.4 nm on specimen axes Rate = 23.49535 A/sec -8.2 1.9 unbinned pixels; 1.6 2.5 nm along two shift axes; -0.4 -2.9 nm on specimen axes Rate = 14.72555 A/sec -6.5 3.2 unbinned pixels; 1.8 1.8 nm along two shift axes; -0.9 -2.4 nm on specimen axes Rate = 12.73783 A/sec Drift is low enough after shot 5 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.39 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.42 nm/sec Defocus = -3.154 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.20.30_batch_tomo_01_1_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 113.5 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.429 -0.928 IS units 2936.9 -283.5 unbinned pixels; need stage 0.007 1.508 if reset Tilting to 48 Correcting tilt backlash Trimming A by 35 35, reference by 20 20 -7.5 -2.8 unbinned pixels; 0.0 2.8 nm along two shift axes; 1.2 -2.5 nm on specimen axes Rate = 14.0727 A/sec -0.8 0.6 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.2 -0.3 nm on specimen axes Rate = 1.65224 A/sec Drift is low enough after shot 2 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.35 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.28 nm/sec Defocus = -3.074 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.21.33_batch_tomo_01_1_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 117.0 In SetExposureForMean 120, change by a factor of 1.0295 would require too small a change in exposure time Image shift 0.464 -1.015 IS units 3206.4 -297.2 unbinned pixels; need stage 0.004 1.709 if reset Tilting to -68 -68.00 degrees 27.8 -6.5 unbinned pixels; 5.4 8.4 nm along two shift axes; 1.4 9.9 nm on specimen axes Rate = 50.02798 A/sec 12.8 0.8 unbinned pixels; 1.2 4.3 nm along two shift axes; -0.7 4.5 nm on specimen axes Rate = 22.57563 A/sec 8.7 -0.3 unbinned pixels; 1.1 2.9 nm along two shift axes; -0.2 3.1 nm on specimen axes Rate = 15.32488 A/sec 11.2 -0.1 unbinned pixels; 1.3 3.7 nm along two shift axes; -0.3 3.9 nm on specimen axes Rate = 19.60802 A/sec 11.5 -0.4 unbinned pixels; 1.5 3.8 nm along two shift axes; -0.2 4.1 nm on specimen axes Rate = 20.25739 A/sec 7.7 -0.8 unbinned pixels; 1.2 2.4 nm along two shift axes; 0.1 2.7 nm on specimen axes Rate = 13.55261 A/sec Drift is low enough after shot 6 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.46 nm/sec Measured defocus = -4.40 microns changed by 0.15 to target drift = 0.69 nm/sec Defocus = -0.080 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.23.09_batch_tomo_01_1_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 122.4 In SetExposureForMean 120, change by a factor of 0.9739 would require too small a change in exposure time Image shift 0.501 -1.101 IS units 3475.2 -316.3 unbinned pixels; need stage 0.002 3.307 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 0.78 microns changed by -5.03 to target drift = 0.45 nm/sec Focus area changed from stored state, axis position -2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_02.st Running script 1 at item 3 (-388.61, -259.09) 17:24:04 Refine ZLP: additional offset = -0.8 eV; total offset = -9.7 eV Item 3: Stage: -388.61 -259.09 -223 Label: 3 Note: Sec 2 - anchor_maps.map Target defocus is: -4.25 um Target defocus is: -4.50 um Target defocus = -4.5 Trimming A by 24 135, reference by 24 135 Angle = -4.40, increment = 0.60, shift = -16.5 -7.2 pixels, -0.039 um Y Trimming A by 34 130, reference by 24 135 Angle = -3.20, increment = 1.80, shift = -70.6 -15.8 pixels, -0.164 um Y Trimming A by 24 135, reference by 24 135 Angle = 0.41, increment = 5.41, shift = -228.7 -42.4 pixels, -0.530 um Y Trimming A by 24 135, reference by 24 135 Angle = 3.00, increment = 8.00, shift = -350.3 -62.9 pixels, -0.811 um Y Rough eucentricity: changing Z by 5.81 to -217.56, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 20 140, reference by 22 140 Angle = 18.00, shift = -75.2, -4.4 pixels, 0.009, -0.172 um, IS = -0.009, 0.172 Trimming A by 22 140, reference by 20 140 Angle = 11.00, shift = -102.6, -10.1 pixels, 0.003, -0.235 um, IS = -0.012, 0.407 Trimming A by 31 135, reference by 22 140 Angle = 4.00, shift = -97.7, -7.7 pixels, 0.007, -0.224 um, IS = -0.020, 0.631 Trimming A by 21 20 20 135, reference by 22 24 20 140 Angle = -4.00, shift = 1.1, -15.6 pixels, -0.036, -0.001 um, IS = 0.016, 0.632 Trimming A by 24 135, reference by 24 135 Angle = -11.00, shift = -90.6, -23.4 pixels, -0.030, -0.211 um, IS = 0.046, 0.844 Trimming A by 26 135, reference by 24 135 Angle = -18.00, shift = -29.4, 0.8 pixels, 0.009, -0.066 um, IS = 0.036, 0.910 Trimming A by 26 135, reference by 26 135 Angle = -24.00, shift = 8.7, 4.6 pixels, 0.008, 0.021 um, IS = 0.028, 0.889 General solution delZ 1.11, delY 1.69, yZero 0.65 solution with assumed YZero 0.63: delZ 1.11, delY 1.40 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 1.11 microns and lateral displacement of -1.69 microns Trimming A by 21 21 20 145, reference by 21 24 20 140 Disparity in stage position after aligning to center of map frame: -0.03 -1.30 Trimming A by 23 22 20 145, reference by 21 24 20 140 Disparity in stage position after aligning to target position: 0.37 -1.73 (2nd move error 0.04 0.11) Disparity in stage position after aligning to map item itself: -0.00 -1.19 (align shift -0.01 -0.03) Finished aligning to item in 3 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -388.63 -260.35 -218.66 0.1 -0.1 unbinned pixels; 0.0 0.0 nm along two shift axes; 0.0 0.1 nm on specimen axes Rate = 0.27272 A/sec Drift is low enough after shot 1 Measured defocus = 1.39 microns changed by -5.89 to target drift = 0.09 nm/sec Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.04 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -1.984 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.29.00_batch_tomo_02_3_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 128.7 In SetExposureForMean 120, exposure time changed to 1.0800 Image shift 0.050 -0.055 IS units 197.1 -83.7 unbinned pixels; need stage 0.023 0.074 if reset Tilting to -8 Correcting tilt backlash 8.0 -0.5 unbinned pixels; 1.1 2.6 nm along two shift axes; -0.1 2.8 nm on specimen axes Rate = 14.07626 A/sec 6.4 1.6 unbinned pixels; 0.2 2.3 nm along two shift axes; -0.8 2.2 nm on specimen axes Rate = 11.65531 A/sec Drift is low enough after shot 2 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.08 nm/sec Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.04 nm/sec Defocus = -1.973 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.30.04_batch_tomo_02_3_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, change by a factor of 1.0362 would require too small a change in exposure time Image shift 0.036 -0.098 IS units 302.6 -5.5 unbinned pixels; need stage -0.007 0.108 if reset Tilting to -12 -12.00 degrees -8.1 0.3 unbinned pixels; 1.0 2.6 nm along two shift axes; 0.2 -2.9 nm on specimen axes Rate = 14.22722 A/sec -4.6 5.1 unbinned pixels; 2.2 0.9 nm along two shift axes; -1.7 -1.8 nm on specimen axes Rate = 12.11491 A/sec Drift is low enough after shot 2 Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.09 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -1.840 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.31.06_batch_tomo_02_3_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, change by a factor of 1.0341 would require too small a change in exposure time Image shift -0.041 0.042 IS units -155.1 70.2 unbinned pixels; need stage -0.019 -0.058 if reset Tilting to -14 8.2 -1.4 unbinned pixels; 1.4 2.5 nm along two shift axes; 0.2 2.9 nm on specimen axes Rate = 14.57746 A/sec 6.8 1.5 unbinned pixels; 0.3 2.4 nm along two shift axes; -0.7 2.3 nm on specimen axes Rate = 12.18801 A/sec Drift is low enough after shot 2 Measured defocus = -4.60 microns changed by 0.10 to target drift = 0.12 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -1.727 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.32.07_batch_tomo_02_3_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, change by a factor of 1.0339 would require too small a change in exposure time Image shift -0.082 0.133 IS units -440.5 97.9 unbinned pixels; need stage -0.020 -0.164 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 9.4 -7.0 unbinned pixels; 3.4 2.3 nm along two shift axes; 2.2 3.5 nm on specimen axes Rate = 20.60281 A/sec 8.1 0.4 unbinned pixels; 0.8 2.7 nm along two shift axes; -0.4 2.8 nm on specimen axes Rate = 14.31135 A/sec 2.8 0.7 unbinned pixels; 0.1 1.0 nm along two shift axes; -0.3 1.0 nm on specimen axes Rate = 5.10421 A/sec Drift is low enough after shot 3 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.09 nm/sec Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.03 nm/sec Defocus = -1.965 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.33.16_batch_tomo_02_3_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, change by a factor of 1.0360 would require too small a change in exposure time Image shift 0.098 -0.233 IS units 727.7 -45.5 unbinned pixels; need stage -0.007 0.260 if reset Tilting to -4 Correcting tilt backlash -6.7 1.6 unbinned pixels; 1.3 2.0 nm along two shift axes; -0.3 -2.4 nm on specimen axes Rate = 11.98973 A/sec Drift is low enough after shot 1 Measured defocus = -4.33 microns changed by -0.17 to target drift = 0.06 nm/sec Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.18 nm/sec Defocus = -2.068 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.34.14_batch_tomo_02_3_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 115.7 In SetExposureForMean 120, change by a factor of 1.0376 would require too small a change in exposure time Image shift 0.097 -0.229 IS units 715.9 -45.4 unbinned pixels; need stage -0.006 0.255 if reset Tilting to -16 -16.00 degrees -5.5 -0.8 unbinned pixels; 0.4 1.9 nm along two shift axes; 0.5 -1.9 nm on specimen axes Rate = 9.77797 A/sec Drift is low enough after shot 1 Measured defocus = -4.65 microns changed by 0.15 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -1.565 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.35.12_batch_tomo_02_3_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 115.7 In SetExposureForMean 120, change by a factor of 1.0370 would require too small a change in exposure time Image shift -0.073 0.084 IS units -299.2 119.0 unbinned pixels; need stage -0.032 -0.114 if reset Tilting to -18 11.0 -6.8 unbinned pixels; 3.5 2.8 nm along two shift axes; 2.0 4.1 nm on specimen axes Rate = 22.63701 A/sec 8.0 0.2 unbinned pixels; 0.9 2.7 nm along two shift axes; -0.3 2.8 nm on specimen axes Rate = 14.1174 A/sec 5.8 0.5 unbinned pixels; 0.5 2.0 nm along two shift axes; -0.4 2.0 nm on specimen axes Rate = 10.18143 A/sec Drift is low enough after shot 3 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.12 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.11 nm/sec Defocus = -1.499 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.36.20_batch_tomo_02_3_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, change by a factor of 1.0368 would require too small a change in exposure time Image shift -0.041 0.041 IS units -152.0 72.7 unbinned pixels; need stage -0.020 -0.059 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 9.8 0.5 unbinned pixels; 1.0 3.3 nm along two shift axes; -0.5 3.4 nm on specimen axes Rate = 17.18114 A/sec 6.0 0.1 unbinned pixels; 0.7 2.0 nm along two shift axes; -0.2 2.1 nm on specimen axes Rate = 10.58286 A/sec Drift is low enough after shot 2 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.09 nm/sec Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.01 nm/sec Defocus = -1.947 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.37.25_batch_tomo_02_3_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 115.3 In SetExposureForMean 120, change by a factor of 1.0407 would require too small a change in exposure time Image shift 0.100 -0.242 IS units 755.4 -42.5 unbinned pixels; need stage -0.009 0.269 if reset Tilting to 0 Correcting tilt backlash -16.0 2.6 unbinned pixels; 2.7 5.0 nm along two shift axes; -0.4 -5.7 nm on specimen axes Rate = 28.44901 A/sec -11.3 5.7 unbinned pixels; 3.2 3.1 nm along two shift axes; -1.7 -4.2 nm on specimen axes Rate = 22.2617 A/sec -9.0 2.1 unbinned pixels; 1.7 2.7 nm along two shift axes; -0.5 -3.2 nm on specimen axes Rate = 16.22162 A/sec -5.7 0.9 unbinned pixels; 1.0 1.8 nm along two shift axes; -0.1 -2.0 nm on specimen axes Rate = 10.06708 A/sec Drift is low enough after shot 4 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.27 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.25 nm/sec Defocus = -2.024 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.38.40_batch_tomo_02_3_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 115.2 In SetExposureForMean 120, change by a factor of 1.0418 would require too small a change in exposure time Image shift 0.065 -0.173 IS units 533.3 -12.5 unbinned pixels; need stage -0.012 0.189 if reset Tilting to -20 -20.00 degrees -2.7 0.3 unbinned pixels; 0.4 0.9 nm along two shift axes; -0.0 -1.0 nm on specimen axes Rate = 4.77004 A/sec Drift is low enough after shot 1 Measured defocus = -4.62 microns changed by 0.12 to target drift = 0.03 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.365 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.39.38_batch_tomo_02_3_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 115.3 In SetExposureForMean 120, change by a factor of 1.0406 would require too small a change in exposure time Image shift -0.052 0.024 IS units -116.8 119.7 unbinned pixels; need stage -0.038 -0.048 if reset Tilting to -22 13.5 -7.1 unbinned pixels; 3.9 3.6 nm along two shift axes; 2.1 5.0 nm on specimen axes Rate = 26.68734 A/sec 10.1 0.8 unbinned pixels; 0.9 3.4 nm along two shift axes; -0.6 3.5 nm on specimen axes Rate = 17.81791 A/sec 5.1 -1.1 unbinned pixels; 1.0 1.6 nm along two shift axes; 0.2 1.8 nm on specimen axes Rate = 9.20739 A/sec Drift is low enough after shot 3 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.10 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.15 nm/sec Defocus = -1.291 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.40.45_batch_tomo_02_3_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 114.9 In SetExposureForMean 120, change by a factor of 1.0443 would require too small a change in exposure time Image shift -0.010 -0.044 IS units 110.2 69.1 unbinned pixels; need stage -0.027 0.040 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 13.2 -1.6 unbinned pixels; 2.1 4.2 nm along two shift axes; 0.1 4.7 nm on specimen axes Rate = 23.38861 A/sec 8.2 -0.4 unbinned pixels; 1.1 2.6 nm along two shift axes; -0.1 2.9 nm on specimen axes Rate = 14.33941 A/sec 1.6 2.3 unbinned pixels; 0.6 0.8 nm along two shift axes; -0.8 0.5 nm on specimen axes Rate = 4.85599 A/sec Drift is low enough after shot 3 Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.07 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -2.061 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.41.56_batch_tomo_02_3_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, change by a factor of 1.0488 would require too small a change in exposure time Image shift 0.056 -0.169 IS units 512.1 9.0 unbinned pixels; need stage -0.019 0.181 if reset Tilting to 4 Correcting tilt backlash -35.1 8.3 unbinned pixels; 6.9 10.6 nm along two shift axes; -1.8 -12.6 nm on specimen axes Rate = 63.22764 A/sec -25.2 8.9 unbinned pixels; 5.9 7.3 nm along two shift axes; -2.3 -9.1 nm on specimen axes Rate = 46.88084 A/sec -20.5 5.7 unbinned pixels; 4.3 6.1 nm along two shift axes; -1.4 -7.4 nm on specimen axes Rate = 37.34428 A/sec -15.6 1.7 unbinned pixels; 2.4 5.0 nm along two shift axes; -0.1 -5.5 nm on specimen axes Rate = 27.5539 A/sec -9.2 2.7 unbinned pixels; 2.0 2.7 nm along two shift axes; -0.7 -3.3 nm on specimen axes Rate = 16.73494 A/sec -8.2 0.9 unbinned pixels; 1.2 2.6 nm along two shift axes; -0.1 -2.9 nm on specimen axes Rate = 14.40614 A/sec -5.1 1.1 unbinned pixels; 0.9 1.6 nm along two shift axes; -0.2 -1.8 nm on specimen axes Rate = 9.10513 A/sec Drift is low enough after shot 7 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.20 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.18 nm/sec Defocus = -2.087 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.43.27_batch_tomo_02_3_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 113.8 In SetExposureForMean 120, change by a factor of 1.0546 would require too small a change in exposure time Image shift -0.010 -0.042 IS units 105.4 67.3 unbinned pixels; need stage -0.026 0.035 if reset Tilting to -24 -24.00 degrees 0.4 -0.6 unbinned pixels; 0.2 0.1 nm along two shift axes; 0.2 0.2 nm on specimen axes Rate = 1.22949 A/sec Drift is low enough after shot 1 Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.08 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -1.176 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.44.25_batch_tomo_02_3_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, change by a factor of 1.0490 would require too small a change in exposure time Image shift -0.007 -0.087 IS units 229.4 103.1 unbinned pixels; need stage -0.043 0.085 if reset Tilting to -26 11.2 -7.2 unbinned pixels; 3.7 2.8 nm along two shift axes; 2.2 4.1 nm on specimen axes Rate = 23.35536 A/sec 10.0 1.3 unbinned pixels; 0.7 3.5 nm along two shift axes; -0.8 3.5 nm on specimen axes Rate = 17.70082 A/sec 6.6 0.1 unbinned pixels; 0.7 2.2 nm along two shift axes; -0.2 2.3 nm on specimen axes Rate = 11.61327 A/sec Drift is low enough after shot 3 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.18 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.13 nm/sec Defocus = -1.115 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.45.32_batch_tomo_02_3_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 113.9 In SetExposureForMean 120, change by a factor of 1.0541 would require too small a change in exposure time Image shift 0.034 -0.150 IS units 440.1 50.3 unbinned pixels; need stage -0.031 0.171 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 20.2 -3.1 unbinned pixels; 3.4 6.3 nm along two shift axes; 0.5 7.2 nm on specimen axes Rate = 35.85737 A/sec 4.5 4.7 unbinned pixels; 1.0 2.0 nm along two shift axes; -1.8 1.4 nm on specimen axes Rate = 11.45463 A/sec Drift is low enough after shot 2 Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.06 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.04 nm/sec Defocus = -2.160 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.46.38_batch_tomo_02_3_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 113.1 In SetExposureForMean 120, exposure time changed to 1.2000 Image shift 0.023 -0.129 IS units 373.9 59.3 unbinned pixels; need stage -0.032 0.131 if reset Tilting to 8 Correcting tilt backlash -46.9 8.6 unbinned pixels; 8.3 14.5 nm along two shift axes; -1.6 -16.7 nm on specimen axes Rate = 83.61885 A/sec -20.3 7.7 unbinned pixels; 4.9 5.8 nm along two shift axes; -2.1 -7.4 nm on specimen axes Rate = 38.06926 A/sec -11.2 1.8 unbinned pixels; 1.9 3.5 nm along two shift axes; -0.3 -4.0 nm on specimen axes Rate = 19.92678 A/sec -11.1 1.1 unbinned pixels; 1.7 3.5 nm along two shift axes; -0.1 -3.9 nm on specimen axes Rate = 19.57841 A/sec -3.3 0.8 unbinned pixels; 0.6 1.0 nm along two shift axes; -0.2 -1.2 nm on specimen axes Rate = 5.94125 A/sec Drift is low enough after shot 5 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.12 nm/sec Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.29 nm/sec Defocus = -2.135 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.47.58_batch_tomo_02_3_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 125.2 In SetExposureForMean 120, change by a factor of 0.9587 would require too small a change in exposure time Image shift -0.010 -0.044 IS units 107.6 69.9 unbinned pixels; need stage -0.027 0.036 if reset Tilting to -28 -28.00 degrees -6.7 -2.2 unbinned pixels; 0.1 2.5 nm along two shift axes; 1.0 -2.3 nm on specimen axes Rate = 12.38003 A/sec Drift is low enough after shot 1 Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.09 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -0.997 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.48.58_batch_tomo_02_3_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 126.1 In SetExposureForMean 120, change by a factor of 0.9519 would require too small a change in exposure time Image shift 0.050 -0.195 IS units 578.6 50.7 unbinned pixels; need stage -0.035 0.230 if reset Tilting to -30 12.1 -6.4 unbinned pixels; 3.5 3.2 nm along two shift axes; 1.9 4.4 nm on specimen axes Rate = 23.94175 A/sec 12.0 1.6 unbinned pixels; 0.9 4.2 nm along two shift axes; -0.9 4.1 nm on specimen axes Rate = 21.19755 A/sec 7.5 1.3 unbinned pixels; 0.5 2.6 nm along two shift axes; -0.7 2.6 nm on specimen axes Rate = 13.34975 A/sec Drift is low enough after shot 3 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.19 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.17 nm/sec Defocus = -0.944 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.50.04_batch_tomo_02_3_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 125.1 In SetExposureForMean 120, change by a factor of 0.9594 would require too small a change in exposure time Image shift 0.081 -0.260 IS units 785.2 27.8 unbinned pixels; need stage -0.034 0.320 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -0.3 -0.9 unbinned pixels; 0.3 0.2 nm along two shift axes; 0.3 -0.1 nm on specimen axes Rate = 1.74757 A/sec Drift is low enough after shot 1 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.21 nm/sec Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.09 nm/sec Defocus = -2.198 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.51.05_batch_tomo_02_3_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 123.9 In SetExposureForMean 120, change by a factor of 0.9682 would require too small a change in exposure time Image shift 0.022 -0.148 IS units 423.5 81.9 unbinned pixels; need stage -0.041 0.149 if reset Tilting to 12 Correcting tilt backlash -17.5 1.6 unbinned pixels; 2.6 5.6 nm along two shift axes; -0.0 -6.2 nm on specimen axes Rate = 30.80776 A/sec -5.5 3.3 unbinned pixels; 1.7 1.4 nm along two shift axes; -1.0 -2.0 nm on specimen axes Rate = 11.19242 A/sec Drift is low enough after shot 2 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.22 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.25 nm/sec Defocus = -2.324 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.52.09_batch_tomo_02_3_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 123.0 In SetExposureForMean 120, change by a factor of 0.9759 would require too small a change in exposure time Image shift 0.001 -0.089 IS units 243.3 81.5 unbinned pixels; need stage -0.036 0.085 if reset Tilting to -32 -32.00 degrees -0.8 -6.4 unbinned pixels; 2.0 1.0 nm along two shift axes; 2.3 -0.1 nm on specimen axes Rate = 11.30585 A/sec Drift is low enough after shot 1 Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.09 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -0.826 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.53.09_batch_tomo_02_3_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 124.5 In SetExposureForMean 120, change by a factor of 0.9635 would require too small a change in exposure time Image shift 0.114 -0.356 IS units 1075.1 29.1 unbinned pixels; need stage -0.043 0.447 if reset Tilting to -34 6.1 -6.8 unbinned pixels; 3.0 1.2 nm along two shift axes; 2.2 2.3 nm on specimen axes Rate = 15.99652 A/sec 4.2 2.6 unbinned pixels; 0.4 1.7 nm along two shift axes; -1.0 1.4 nm on specimen axes Rate = 8.77544 A/sec Drift is low enough after shot 2 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.02 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.803 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.54.16_batch_tomo_02_3_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 123.5 In SetExposureForMean 120, change by a factor of 0.9712 would require too small a change in exposure time Image shift 0.143 -0.405 IS units 1239.5 -3.8 unbinned pixels; need stage -0.037 0.529 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -4.6 -2.2 unbinned pixels; 0.2 1.8 nm along two shift axes; 0.9 -1.5 nm on specimen axes Rate = 8.91937 A/sec Drift is low enough after shot 1 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.11 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -2.434 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.55.18_batch_tomo_02_3_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 122.3 In SetExposureForMean 120, change by a factor of 0.9813 would require too small a change in exposure time Image shift 0.046 -0.190 IS units 559.0 55.4 unbinned pixels; need stage -0.036 0.202 if reset Tilting to 16 Correcting tilt backlash -3.1 -5.0 unbinned pixels; 1.3 1.6 nm along two shift axes; 1.9 -0.9 nm on specimen axes Rate = 10.47899 A/sec Drift is low enough after shot 1 Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.03 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -2.477 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.56.16_batch_tomo_02_3_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 121.1 In SetExposureForMean 120, change by a factor of 0.9912 would require too small a change in exposure time Image shift 0.088 -0.255 IS units 777.9 4.7 unbinned pixels; need stage -0.026 0.286 if reset Tilting to -36 -36.00 degrees 7.2 -5.2 unbinned pixels; 2.6 1.8 nm along two shift axes; 1.6 2.7 nm on specimen axes Rate = 15.54523 A/sec 8.3 5.1 unbinned pixels; 0.7 3.4 nm along two shift axes; -2.1 2.8 nm on specimen axes Rate = 17.23799 A/sec 8.0 1.4 unbinned pixels; 0.5 2.8 nm along two shift axes; -0.7 2.8 nm on specimen axes Rate = 14.34067 A/sec 5.5 0.6 unbinned pixels; 0.4 1.9 nm along two shift axes; -0.4 1.9 nm on specimen axes Rate = 9.68479 A/sec Drift is low enough after shot 4 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.23 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.17 nm/sec Defocus = -0.764 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.57.32_batch_tomo_02_3_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 122.0 In SetExposureForMean 120, change by a factor of 0.9835 would require too small a change in exposure time Image shift 0.184 -0.532 IS units 1623.4 7.1 unbinned pixels; need stage -0.052 0.709 if reset Tilting to -38 5.3 -6.8 unbinned pixels; 2.9 0.9 nm along two shift axes; 2.2 2.1 nm on specimen axes Rate = 15.12183 A/sec 4.4 1.7 unbinned pixels; 0.0 1.6 nm along two shift axes; -0.7 1.5 nm on specimen axes Rate = 8.23056 A/sec Drift is low enough after shot 2 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.02 nm/sec Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.09 nm/sec Defocus = -0.612 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.58.34_batch_tomo_02_3_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 120.9 In SetExposureForMean 120, change by a factor of 0.9922 would require too small a change in exposure time Image shift 0.215 -0.578 IS units 1777.9 -34.2 unbinned pixels; need stage -0.043 0.799 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -10.6 1.2 unbinned pixels; 1.6 3.4 nm along two shift axes; -0.1 -3.7 nm on specimen axes Rate = 18.66006 A/sec -9.2 6.1 unbinned pixels; 3.1 2.3 nm along two shift axes; -1.9 -3.4 nm on specimen axes Rate = 19.38213 A/sec -2.3 2.2 unbinned pixels; 1.0 0.5 nm along two shift axes; -0.7 -0.9 nm on specimen axes Rate = 5.55357 A/sec Drift is low enough after shot 3 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.03 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.32 nm/sec Defocus = -2.474 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_17.59.46_batch_tomo_02_3_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0062 would require too small a change in exposure time Image shift 0.055 -0.235 IS units 690.6 72.6 unbinned pixels; need stage -0.046 0.254 if reset Tilting to 20 Correcting tilt backlash -11.6 -0.9 unbinned pixels; 1.1 3.9 nm along two shift axes; 0.7 -4.0 nm on specimen axes Rate = 20.38496 A/sec -3.9 5.7 unbinned pixels; 2.3 0.6 nm along two shift axes; -1.9 -1.5 nm on specimen axes Rate = 12.06702 A/sec Drift is low enough after shot 2 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.16 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.30 nm/sec Defocus = -2.563 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.00.55_batch_tomo_02_3_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0210 would require too small a change in exposure time Image shift 0.082 -0.310 IS units 920.2 70.8 unbinned pixels; need stage -0.053 0.344 if reset Tilting to -40 -40.00 degrees 25.9 -6.6 unbinned pixels; 5.2 7.8 nm along two shift axes; 1.5 9.3 nm on specimen axes Rate = 46.75003 A/sec 5.5 1.9 unbinned pixels; 0.0 2.1 nm along two shift axes; -0.8 1.9 nm on specimen axes Rate = 10.2645 A/sec Drift is low enough after shot 2 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.04 nm/sec Measured defocus = -4.61 microns changed by 0.11 to target drift = 0.10 nm/sec Defocus = -0.516 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.02.01_batch_tomo_02_3_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 119.7 In SetExposureForMean 120, change by a factor of 1.0020 would require too small a change in exposure time Image shift 0.245 -0.687 IS units 2102.3 -13.3 unbinned pixels; need stage -0.060 0.971 if reset Tilting to -42 14.4 -5.3 unbinned pixels; 3.4 4.1 nm along two shift axes; 1.4 5.2 nm on specimen axes Rate = 26.95282 A/sec 8.7 1.4 unbinned pixels; 0.6 3.0 nm along two shift axes; -0.7 3.0 nm on specimen axes Rate = 15.44266 A/sec -3.5 -1.7 unbinned pixels; 0.2 1.4 nm along two shift axes; 0.7 -1.2 nm on specimen axes Rate = 6.95605 A/sec Drift is low enough after shot 3 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.33 nm/sec Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.45 nm/sec Defocus = -0.674 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.03.08_batch_tomo_02_3_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 118.1 In SetExposureForMean 120, change by a factor of 1.0156 would require too small a change in exposure time Image shift 0.262 -0.711 IS units 2183.9 -35.6 unbinned pixels; need stage -0.055 1.041 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -4.1 1.6 unbinned pixels; 1.0 1.2 nm along two shift axes; -0.4 -1.5 nm on specimen axes Rate = 7.62674 A/sec Drift is low enough after shot 1 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.57 nm/sec Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.20 nm/sec Defocus = -2.613 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.04.10_batch_tomo_02_3_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, change by a factor of 1.0364 would require too small a change in exposure time Image shift 0.052 -0.247 IS units 720.2 93.2 unbinned pixels; need stage -0.054 0.271 if reset Tilting to 24 Correcting tilt backlash -7.0 1.8 unbinned pixels; 1.4 2.1 nm along two shift axes; -0.4 -2.5 nm on specimen axes Rate = 12.66485 A/sec Drift is low enough after shot 1 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.32 nm/sec Measured defocus = -4.22 microns changed by -0.28 to target drift = 0.04 nm/sec Defocus = -2.841 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.05.08_batch_tomo_02_3_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 113.8 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift 0.077 -0.329 IS units 965.4 104.4 unbinned pixels; need stage -0.066 0.370 if reset Tilting to -44 -44.00 degrees 5.8 -7.8 unbinned pixels; 3.3 1.0 nm along two shift axes; 2.6 2.3 nm on specimen axes Rate = 17.10136 A/sec 4.3 1.3 unbinned pixels; 0.1 1.6 nm along two shift axes; -0.6 1.5 nm on specimen axes Rate = 8.00353 A/sec Drift is low enough after shot 2 Measured defocus = -4.66 microns changed by 0.16 to target drift = 0.26 nm/sec Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.09 nm/sec Defocus = -0.463 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.06.14_batch_tomo_02_3_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 128.0 In SetExposureForMean 120, exposure time changed to 1.2000 Image shift 0.311 -0.817 IS units 2521.0 -69.5 unbinned pixels; need stage -0.054 1.242 if reset Tilting to -46 11.7 -1.4 unbinned pixels; 1.8 3.7 nm along two shift axes; 0.1 4.1 nm on specimen axes Rate = 20.66503 A/sec 7.8 -0.6 unbinned pixels; 1.1 2.5 nm along two shift axes; -0.0 2.8 nm on specimen axes Rate = 13.71592 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.34 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.33 nm/sec Defocus = -0.406 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.07.16_batch_tomo_02_3_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, change by a factor of 1.0475 would require too small a change in exposure time Image shift 0.319 -0.830 IS units 2563.7 -76.9 unbinned pixels; need stage -0.052 1.309 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -22.7 5.4 unbinned pixels; 4.4 6.9 nm along two shift axes; -1.2 -8.1 nm on specimen axes Rate = 40.86218 A/sec -7.5 2.7 unbinned pixels; 1.8 2.1 nm along two shift axes; -0.7 -2.7 nm on specimen axes Rate = 13.94786 A/sec Drift is low enough after shot 2 Measured defocus = -4.67 microns changed by 0.17 to target drift = 0.13 nm/sec Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.47 nm/sec Defocus = -2.593 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.08.25_batch_tomo_02_3_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 111.5 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift 0.054 -0.256 IS units 745.4 97.1 unbinned pixels; need stage -0.056 0.290 if reset Tilting to 28 Correcting tilt backlash 4.7 -4.4 unbinned pixels; 2.0 1.0 nm along two shift axes; 1.4 1.8 nm on specimen axes Rate = 11.30254 A/sec Drift is low enough after shot 1 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.13 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.35 nm/sec Defocus = -2.717 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.09.23_batch_tomo_02_3_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 120.5 In SetExposureForMean 120, change by a factor of 0.9961 would require too small a change in exposure time Image shift 0.095 -0.375 IS units 1110.1 99.0 unbinned pixels; need stage -0.068 0.441 if reset Tilting to -48 -48.00 degrees 23.5 -6.2 unbinned pixels; 4.8 7.0 nm along two shift axes; 1.4 8.4 nm on specimen axes Rate = 42.57052 A/sec 19.7 2.1 unbinned pixels; 1.6 6.8 nm along two shift axes; -1.3 6.8 nm on specimen axes Rate = 34.7166 A/sec 8.3 0.6 unbinned pixels; 0.8 2.8 nm along two shift axes; -0.5 2.9 nm on specimen axes Rate = 14.58403 A/sec 4.9 0.2 unbinned pixels; 0.5 1.6 nm along two shift axes; -0.2 1.7 nm on specimen axes Rate = 8.53957 A/sec Drift is low enough after shot 4 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.22 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.43 nm/sec Defocus = -0.353 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.10.41_batch_tomo_02_3_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 123.5 In SetExposureForMean 120, change by a factor of 0.9692 would require too small a change in exposure time Image shift 0.353 -0.907 IS units 2803.7 -97.1 unbinned pixels; need stage -0.053 1.486 if reset Tilting to -50 5.6 -5.6 unbinned pixels; 2.5 1.2 nm along two shift axes; 1.8 2.1 nm on specimen axes Rate = 13.90506 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.39 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.27 nm/sec Defocus = -0.301 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.11.37_batch_tomo_02_3_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 121.2 In SetExposureForMean 120, change by a factor of 0.9869 would require too small a change in exposure time Image shift 0.373 -0.937 IS units 2904.8 -122.8 unbinned pixels; need stage -0.047 1.604 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -21.2 7.5 unbinned pixels; 5.0 6.1 nm along two shift axes; -2.0 -7.7 nm on specimen axes Rate = 39.35469 A/sec -9.0 0.7 unbinned pixels; 1.3 2.9 nm along two shift axes; 0.0 -3.2 nm on specimen axes Rate = 15.76353 A/sec -7.2 2.4 unbinned pixels; 1.6 2.1 nm along two shift axes; -0.6 -2.6 nm on specimen axes Rate = 13.21992 A/sec Drift is low enough after shot 3 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.08 nm/sec Measured defocus = -4.32 microns changed by -0.18 to target drift = 0.30 nm/sec Defocus = -2.863 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.12.51_batch_tomo_02_3_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 117.1 In SetExposureForMean 120, change by a factor of 1.0252 would require too small a change in exposure time Image shift 0.082 -0.336 IS units 991.4 95.2 unbinned pixels; need stage -0.063 0.401 if reset Tilting to 32 Correcting tilt backlash -8.9 -5.8 unbinned pixels; 0.9 3.7 nm along two shift axes; 2.3 -3.0 nm on specimen axes Rate = 18.83285 A/sec -5.7 2.9 unbinned pixels; 1.6 1.5 nm along two shift axes; -0.8 -2.1 nm on specimen axes Rate = 11.22519 A/sec Drift is low enough after shot 2 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.37 nm/sec Measured defocus = -4.74 microns changed by 0.24 to target drift = 0.39 nm/sec Defocus = -2.723 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.13.55_batch_tomo_02_3_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 114.5 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.129 -0.463 IS units 1381.2 89.2 unbinned pixels; need stage -0.073 0.572 if reset Tilting to -52 -52.00 degrees -7.4 -9.0 unbinned pixels; 2.1 3.5 nm along two shift axes; 3.4 -2.3 nm on specimen axes Rate = 20.61602 A/sec 31.5 2.0 unbinned pixels; 3.0 10.7 nm along two shift axes; -1.7 11.0 nm on specimen axes Rate = 55.37727 A/sec -12.4 5.5 unbinned pixels; 3.3 3.4 nm along two shift axes; -1.6 -4.5 nm on specimen axes Rate = 23.70855 A/sec 14.7 -0.8 unbinned pixels; 2.0 4.8 nm along two shift axes; -0.2 5.2 nm on specimen axes Rate = 25.82431 A/sec 35.9 -1.0 unbinned pixels; 4.5 11.8 nm along two shift axes; -0.8 12.6 nm on specimen axes Rate = 63.01825 A/sec -3.8 -0.7 unbinned pixels; 0.2 1.3 nm along two shift axes; 0.4 -1.3 nm on specimen axes Rate = 6.70829 A/sec Drift is low enough after shot 6 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.66 nm/sec Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.05 nm/sec Defocus = -0.176 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.15.25_batch_tomo_02_3_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 129.8 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift 0.410 -1.020 IS units 3167.7 -145.6 unbinned pixels; need stage -0.047 1.827 if reset Tilting to -54 11.6 -5.9 unbinned pixels; 3.3 3.1 nm along two shift axes; 1.7 4.3 nm on specimen axes Rate = 22.8514 A/sec 1.3 5.0 unbinned pixels; 1.5 1.0 nm along two shift axes; -1.8 0.3 nm on specimen axes Rate = 9.0508 A/sec Drift is low enough after shot 2 Measured defocus = -4.67 microns changed by 0.17 to target drift = 0.38 nm/sec Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.33 nm/sec Defocus = -0.058 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.16.27_batch_tomo_02_3_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 116.4 In SetExposureForMean 120, change by a factor of 1.0245 would require too small a change in exposure time Image shift 0.430 -1.067 IS units 3313.7 -156.1 unbinned pixels; need stage -0.048 2.002 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -17.6 2.0 unbinned pixels; 2.7 5.6 nm along two shift axes; -0.2 -6.2 nm on specimen axes Rate = 31.02964 A/sec -18.4 6.5 unbinned pixels; 4.3 5.3 nm along two shift axes; -1.7 -6.6 nm on specimen axes Rate = 34.14195 A/sec -6.4 2.5 unbinned pixels; 1.6 1.8 nm along two shift axes; -0.7 -2.3 nm on specimen axes Rate = 12.11008 A/sec Drift is low enough after shot 3 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.26 nm/sec Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.41 nm/sec Defocus = -2.697 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.17.42_batch_tomo_02_3_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 110.5 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.131 -0.438 IS units 1316.5 59.5 unbinned pixels; need stage -0.061 0.559 if reset Tilting to 36 Correcting tilt backlash -14.7 -2.9 unbinned pixels; 0.8 5.2 nm along two shift axes; 1.5 -5.1 nm on specimen axes Rate = 26.27286 A/sec -1.3 3.1 unbinned pixels; 1.2 0.1 nm along two shift axes; -1.0 -0.6 nm on specimen axes Rate = 5.86788 A/sec Drift is low enough after shot 2 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.43 nm/sec Defocus = -2.739 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.18.46_batch_tomo_02_3_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 117.1 In SetExposureForMean 120, change by a factor of 1.0244 would require too small a change in exposure time Image shift 0.164 -0.539 IS units 1622.5 65.3 unbinned pixels; need stage -0.072 0.706 if reset Tilting to -56 -56.00 degrees 10.0 -8.8 unbinned pixels; 4.1 2.3 nm along two shift axes; 2.8 3.8 nm on specimen axes Rate = 23.38916 A/sec 2.8 1.9 unbinned pixels; 0.3 1.1 nm along two shift axes; -0.8 0.9 nm on specimen axes Rate = 5.95494 A/sec Drift is low enough after shot 2 Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.41 nm/sec Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.21 nm/sec Defocus = -0.053 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.19.54_batch_tomo_02_3_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 124.0 In SetExposureForMean 120, change by a factor of 0.9596 would require too small a change in exposure time Image shift 0.483 -1.183 IS units 3682.0 -189.3 unbinned pixels; need stage -0.048 2.339 if reset Tilting to -58 -1.9 -0.5 unbinned pixels; 0.0 0.7 nm along two shift axes; 0.2 -0.6 nm on specimen axes Rate = 3.40365 A/sec Drift is low enough after shot 1 Measured defocus = -4.66 microns changed by 0.16 to target drift = 0.33 nm/sec Measured defocus = -4.18 microns changed by -0.32 to target drift = 0.37 nm/sec Defocus = -0.212 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.20.50_batch_tomo_02_3_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 120.5 In SetExposureForMean 120, change by a factor of 0.9852 would require too small a change in exposure time Image shift 0.491 -1.200 IS units 3735.3 -193.6 unbinned pixels; need stage -0.048 2.504 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -24.4 5.1 unbinned pixels; 4.5 7.5 nm along two shift axes; -1.0 -8.7 nm on specimen axes Rate = 43.6714 A/sec -5.7 3.3 unbinned pixels; 1.8 1.5 nm along two shift axes; -1.0 -2.1 nm on specimen axes Rate = 11.54495 A/sec Drift is low enough after shot 2 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.11 nm/sec Measured defocus = -4.65 microns changed by 0.15 to target drift = 0.51 nm/sec Defocus = -2.639 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.22.00_batch_tomo_02_3_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 112.2 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.166 -0.524 IS units 1581.5 47.7 unbinned pixels; need stage -0.065 0.708 if reset Tilting to 40 Correcting tilt backlash Trimming A by 25 30, reference by 20 20 -6.1 -3.7 unbinned pixels; 0.5 2.5 nm along two shift axes; 1.5 -2.0 nm on specimen axes Rate = 12.55203 A/sec Drift is low enough after shot 1 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.29 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.32 nm/sec Defocus = -2.699 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.23.04_batch_tomo_02_3_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, change by a factor of 1.0330 would require too small a change in exposure time Image shift 0.188 -0.600 IS units 1810.0 59.2 unbinned pixels; need stage -0.076 0.833 if reset Tilting to -60 -60.00 degrees 24.4 -9.7 unbinned pixels; 6.1 6.9 nm along two shift axes; 2.7 8.9 nm on specimen axes Rate = 46.07876 A/sec -11.0 1.7 unbinned pixels; 1.8 3.4 nm along two shift axes; -0.2 -3.9 nm on specimen axes Rate = 19.41849 A/sec 17.9 -0.2 unbinned pixels; 2.1 5.9 nm along two shift axes; -0.5 6.3 nm on specimen axes Rate = 31.40041 A/sec -1.5 1.4 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.4 -0.6 nm on specimen axes Rate = 3.67644 A/sec Drift is low enough after shot 4 Measured defocus = -4.89 microns changed by 0.39 to target drift = 0.01 nm/sec Measured defocus = -4.12 microns changed by -0.38 to target drift = 0.23 nm/sec Defocus = -0.209 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.24.23_batch_tomo_02_3_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 126.1 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.533 -1.296 IS units 4035.8 -217.5 unbinned pixels; need stage -0.049 2.868 if reset Tilting to -62 53.2 -8.7 unbinned pixels; 9.1 16.6 nm along two shift axes; 1.4 18.9 nm on specimen axes Rate = 94.54482 A/sec 17.7 2.7 unbinned pixels; 1.2 6.2 nm along two shift axes; -1.5 6.1 nm on specimen axes Rate = 31.45896 A/sec 4.6 1.0 unbinned pixels; 0.2 1.6 nm along two shift axes; -0.5 1.6 nm on specimen axes Rate = 8.31939 A/sec Drift is low enough after shot 3 Measured defocus = -4.97 microns changed by 0.47 to target drift = 0.44 nm/sec Measured defocus = -4.32 microns changed by -0.18 to target drift = 0.51 nm/sec Defocus = 0.082 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.25.30_batch_tomo_02_3_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 112.1 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.560 -1.355 IS units 4222.7 -236.4 unbinned pixels; need stage -0.049 3.197 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees Trimming A by 20 25, reference by 20 20 -24.0 5.6 unbinned pixels; 4.6 7.3 nm along two shift axes; -1.2 -8.6 nm on specimen axes Rate = 43.14305 A/sec Trimming A by 25 25, reference by 20 20 -15.6 4.3 unbinned pixels; 3.3 4.7 nm along two shift axes; -1.0 -5.6 nm on specimen axes Rate = 28.4205 A/sec Trimming A by 25 25, reference by 20 20 -3.1 0.2 unbinned pixels; 0.4 1.0 nm along two shift axes; 0.0 -1.1 nm on specimen axes Rate = 5.48153 A/sec Drift is low enough after shot 3 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.22 nm/sec Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.28 nm/sec Defocus = -2.589 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.26.46_batch_tomo_02_3_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 109.7 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.188 -0.585 IS units 1770.0 45.3 unbinned pixels; need stage -0.070 0.840 if reset Tilting to 44 Correcting tilt backlash Trimming A by 40 45, reference by 20 20 Trimming A by 25 25, reference by 20 20 -24.4 0.5 unbinned pixels; 3.0 8.0 nm along two shift axes; 0.6 -8.6 nm on specimen axes Rate = 42.76621 A/sec Trimming A by 25 25, reference by 20 20 -5.0 0.3 unbinned pixels; 0.7 1.6 nm along two shift axes; 0.1 -1.8 nm on specimen axes Rate = 8.75264 A/sec Drift is low enough after shot 2 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.50 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.33 nm/sec Defocus = -2.644 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.27.51_batch_tomo_02_3_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 112.2 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.220 -0.674 IS units 2044.0 42.2 unbinned pixels; need stage -0.077 1.003 if reset Tilting to -64 -64.00 degrees 92.0 -11.9 unbinned pixels; 14.7 29.0 nm along two shift axes; 1.3 32.6 nm on specimen axes Rate = 162.58556 A/sec 6.8 -1.5 unbinned pixels; 1.3 2.1 nm along two shift axes; 0.3 2.4 nm on specimen axes Rate = 12.20293 A/sec Drift is low enough after shot 2 Measured defocus = -4.08 microns changed by -0.42 to target drift = 0.52 nm/sec Measured defocus = -4.80 microns changed by 0.30 to target drift = 0.52 nm/sec Defocus = -0.041 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.29.09_batch_tomo_02_3_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 134.7 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.613 -1.474 IS units 4598.0 -266.4 unbinned pixels; need stage -0.050 3.728 if reset Tilting to -66 11.6 -7.7 unbinned pixels; 3.9 2.9 nm along two shift axes; 2.3 4.3 nm on specimen axes Rate = 24.34769 A/sec 101.6 -5.0 unbinned pixels; 13.5 33.0 nm along two shift axes; -1.4 35.8 nm on specimen axes Rate = 178.36834 A/sec 7.0 0.1 unbinned pixels; 0.8 2.3 nm along two shift axes; -0.2 2.4 nm on specimen axes Rate = 12.26409 A/sec Drift is low enough after shot 3 Measured defocus = -4.18 microns changed by -0.32 to target drift = 0.12 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.21 nm/sec Defocus = -0.383 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.30.18_batch_tomo_02_3_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 111.4 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.654 -1.540 IS units 4817.4 -313.8 unbinned pixels; need stage -0.040 4.213 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees Trimming A by 25 30, reference by 20 20 Trimming A by 20 25, reference by 20 20 -10.0 -0.5 unbinned pixels; 1.0 3.4 nm along two shift axes; 0.5 -3.5 nm on specimen axes Rate = 17.5971 A/sec Trimming A by 20 25, reference by 20 20 -8.5 4.5 unbinned pixels; 2.5 2.3 nm along two shift axes; -1.3 -3.1 nm on specimen axes Rate = 16.79347 A/sec Trimming A by 25 25, reference by 20 20 -6.5 3.4 unbinned pixels; 1.9 1.8 nm along two shift axes; -1.0 -2.4 nm on specimen axes Rate = 12.87119 A/sec Drift is low enough after shot 3 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.67 nm/sec Measured defocus = -4.76 microns changed by 0.26 to target drift = 0.22 nm/sec Defocus = -2.400 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.31.34_batch_tomo_02_3_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 105.1 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift 0.248 -0.709 IS units 2165.7 -1.4 unbinned pixels; need stage -0.066 1.102 if reset Tilting to 48 Correcting tilt backlash Trimming A by 25 30, reference by 20 20 Trimming A by 20 25, reference by 20 20 -4.0 0.8 unbinned pixels; 0.7 1.2 nm along two shift axes; -0.2 -1.4 nm on specimen axes Rate = 7.10265 A/sec Drift is low enough after shot 1 Measured defocus = -4.20 microns changed by -0.30 to target drift = 0.60 nm/sec Measured defocus = -4.73 microns changed by 0.23 to target drift = 0.45 nm/sec Defocus = -2.473 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.32.33_batch_tomo_02_3_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 112.2 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.263 -0.750 IS units 2290.1 -1.8 unbinned pixels; need stage -0.070 1.210 if reset Tilting to -68 -68.00 degrees 9.5 -14.6 unbinned pixels; 5.9 1.4 nm along two shift axes; 4.8 3.8 nm on specimen axes Rate = 30.53981 A/sec 29.0 1.0 unbinned pixels; 3.1 9.7 nm along two shift axes; -1.2 10.2 nm on specimen axes Rate = 51.00033 A/sec 9.2 -0.6 unbinned pixels; 1.3 3.0 nm along two shift axes; -0.1 3.3 nm on specimen axes Rate = 16.22067 A/sec 11.1 0.0 unbinned pixels; 1.3 3.7 nm along two shift axes; -0.3 3.9 nm on specimen axes Rate = 19.46176 A/sec 10.3 0.6 unbinned pixels; 1.0 3.5 nm along two shift axes; -0.5 3.6 nm on specimen axes Rate = 18.18372 A/sec 19.2 -1.4 unbinned pixels; 2.7 6.2 nm along two shift axes; -0.1 6.8 nm on specimen axes Rate = 33.83375 A/sec 4.8 -1.1 unbinned pixels; 0.9 1.5 nm along two shift axes; 0.2 1.7 nm on specimen axes Rate = 8.65976 A/sec Drift is low enough after shot 7 Measured defocus = -4.06 microns changed by -0.44 to target drift = 0.36 nm/sec Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.27 nm/sec Defocus = -0.874 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.34.10_batch_tomo_02_3_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 138.3 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.717 -1.674 IS units 5241.6 -360.0 unbinned pixels; need stage -0.037 4.978 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Using peak at 1.2,-3.7, rejecting 1.28 times higher peak at 0.2,-0.2 Measured defocus = 0.21 microns changed by -4.71 to target drift = 0.50 nm/sec Focus area changed from stored state, axis position 2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_03.st Running script 1 at item 5 (-386.49, -268.77) 18:35:05 Refine ZLP: additional offset = -1.1 eV; total offset = -10.8 eV Item 5: Stage: -386.49 -268.77 -223 Label: 5 Note: Sec 4 - anchor_maps.map Target defocus is: -4.50 um Target defocus is: -3.50 um Target defocus = -3.5 Trimming A by 20 25, reference by 20 25 Angle = -4.40, increment = 0.60, shift = -12.4 -6.1 pixels, -0.030 um Y Trimming A by 20 25, reference by 20 25 Angle = -3.20, increment = 1.80, shift = -43.3 -13.2 pixels, -0.102 um Y Trimming A by 20 20, reference by 20 25 Angle = 0.41, increment = 5.41, shift = -122.2 -26.6 pixels, -0.284 um Y Trimming A by 20 20, reference by 20 25 Angle = 3.00, increment = 8.00, shift = -166.7 -38.7 pixels, -0.388 um Y Rough eucentricity: changing Z by 2.78 to -220.59, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = -10.1, 2.4 pixels, 0.008, -0.022 um, IS = -0.008, 0.022 Angle = 11.00, shift = 5.6, 1.9 pixels, 0.003, 0.013 um, IS = -0.011, 0.009 Angle = 4.00, shift = 7.0, 4.1 pixels, 0.007, 0.017 um, IS = -0.018, -0.008 Angle = -4.00, shift = -16.3, -25.0 pixels, -0.053, -0.043 um, IS = 0.034, 0.036 Angle = -11.00, shift = 39.2, 0.2 pixels, -0.010, 0.089 um, IS = 0.044, -0.054 Angle = -18.00, shift = 89.9, 18.1 pixels, 0.018, 0.209 um, IS = 0.026, -0.262 Angle = -24.00, shift = 97.2, 17.7 pixels, 0.015, 0.225 um, IS = 0.010, -0.487 General solution delZ -0.49, delY 3.08, yZero 0.03 solution with assumed YZero 0.01: delZ -0.49, delY 2.87 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.49 microns and lateral displacement of -3.08 microns Trimming A by 30 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.01 -0.91 Aligning to target with image shift equivalent to stage shift: -0.01 -0.91 Disparity in stage position after aligning to map item itself: -0.01 -0.88 (align shift -0.01 0.00) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -386.48 -268.74 -220.10 0.1 0.4 unbinned pixels; 0.1 0.1 nm along two shift axes; -0.1 0.0 nm on specimen axes Rate = 0.71601 A/sec Drift is low enough after shot 1 Measured defocus = 1.42 microns changed by -4.92 to target drift = 0.05 nm/sec Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -1.114 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.39.58_batch_tomo_03_5_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 102.6 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.382 0.796 IS units -2534.3 282.7 unbinned pixels; need stage -0.019 -0.919 if reset Tilting to -8 Correcting tilt backlash 13.5 0.1 unbinned pixels; 1.6 4.5 nm along two shift axes; -0.4 4.7 nm on specimen axes Rate = 23.69221 A/sec 5.7 1.0 unbinned pixels; 0.3 2.0 nm along two shift axes; -0.5 2.0 nm on specimen axes Rate = 10.1997 A/sec Drift is low enough after shot 2 Measured defocus = -3.36 microns changed by -0.14 to target drift = 0.10 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -1.242 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.41.02_batch_tomo_03_5_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 123.3 In SetExposureForMean 120, change by a factor of 0.9732 would require too small a change in exposure time Image shift -0.422 0.800 IS units -2582.0 386.9 unbinned pixels; need stage -0.054 -0.935 if reset Tilting to -12 -12.00 degrees -8.2 0.6 unbinned pixels; 1.2 2.6 nm along two shift axes; 0.0 -2.9 nm on specimen axes Rate = 14.45648 A/sec -3.6 4.8 unbinned pixels; 2.0 0.6 nm along two shift axes; -1.6 -1.4 nm on specimen axes Rate = 10.50483 A/sec Drift is low enough after shot 2 Measured defocus = -3.65 microns changed by 0.15 to target drift = 0.08 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -0.963 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.42.04_batch_tomo_03_5_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 122.7 In SetExposureForMean 120, change by a factor of 0.9787 would require too small a change in exposure time Image shift -0.452 0.831 IS units -2697.3 439.2 unbinned pixels; need stage -0.068 -0.990 if reset Tilting to -14 14.8 -2.4 unbinned pixels; 2.5 4.6 nm along two shift axes; 0.4 5.3 nm on specimen axes Rate = 26.29745 A/sec 6.6 -2.3 unbinned pixels; 1.5 1.9 nm along two shift axes; 0.6 2.4 nm on specimen axes Rate = 12.28636 A/sec Drift is low enough after shot 2 Measured defocus = -3.59 microns changed by 0.09 to target drift = 0.16 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.10 nm/sec Defocus = -0.869 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.43.06_batch_tomo_03_5_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 122.6 In SetExposureForMean 120, change by a factor of 0.9796 would require too small a change in exposure time Image shift -0.433 0.787 IS units -2559.9 429.8 unbinned pixels; need stage -0.069 -0.947 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 10.9 -9.6 unbinned pixels; 4.5 2.4 nm along two shift axes; 3.0 4.1 nm on specimen axes Rate = 25.46911 A/sec 7.0 1.8 unbinned pixels; 0.2 2.5 nm along two shift axes; -0.8 2.4 nm on specimen axes Rate = 12.69815 A/sec Drift is low enough after shot 2 Measured defocus = -3.39 microns changed by -0.11 to target drift = 0.11 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -1.350 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.44.10_batch_tomo_03_5_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 123.3 In SetExposureForMean 120, change by a factor of 0.9736 would require too small a change in exposure time Image shift -0.354 0.639 IS units -2079.1 356.4 unbinned pixels; need stage -0.059 -0.751 if reset Tilting to -4 Correcting tilt backlash -8.4 1.4 unbinned pixels; 1.5 2.6 nm along two shift axes; -0.3 -3.0 nm on specimen axes Rate = 14.87628 A/sec -2.1 1.5 unbinned pixels; 0.7 0.5 nm along two shift axes; -0.4 -0.8 nm on specimen axes Rate = 4.53295 A/sec Drift is low enough after shot 2 Measured defocus = -3.40 microns changed by -0.10 to target drift = 0.06 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -1.444 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.45.15_batch_tomo_03_5_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 122.9 In SetExposureForMean 120, change by a factor of 0.9762 would require too small a change in exposure time Image shift -0.373 0.680 IS units -2208.7 368.5 unbinned pixels; need stage -0.059 -0.795 if reset Tilting to -16 -16.00 degrees -4.7 0.5 unbinned pixels; 0.7 1.5 nm along two shift axes; -0.0 -1.7 nm on specimen axes Rate = 8.26889 A/sec Drift is low enough after shot 1 Measured defocus = -3.65 microns changed by 0.15 to target drift = 0.07 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.715 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.46.13_batch_tomo_03_5_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 121.7 In SetExposureForMean 120, change by a factor of 0.9867 would require too small a change in exposure time Image shift -0.441 0.771 IS units -2523.6 468.3 unbinned pixels; need stage -0.083 -0.944 if reset Tilting to -18 15.5 -9.4 unbinned pixels; 4.9 4.0 nm along two shift axes; 2.8 5.7 nm on specimen axes Rate = 31.74644 A/sec 9.8 -0.1 unbinned pixels; 1.2 3.2 nm along two shift axes; -0.3 3.4 nm on specimen axes Rate = 17.25377 A/sec 6.8 -0.3 unbinned pixels; 0.9 2.2 nm along two shift axes; -0.1 2.4 nm on specimen axes Rate = 11.89675 A/sec Drift is low enough after shot 3 Measured defocus = -3.60 microns changed by 0.10 to target drift = 0.12 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -0.621 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.47.21_batch_tomo_03_5_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 121.1 In SetExposureForMean 120, change by a factor of 0.9917 would require too small a change in exposure time Image shift -0.370 0.642 IS units -2104.8 396.2 unbinned pixels; need stage -0.072 -0.796 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 8.7 -2.8 unbinned pixels; 2.0 2.6 nm along two shift axes; 0.7 3.2 nm on specimen axes Rate = 16.08964 A/sec 7.2 0.1 unbinned pixels; 0.8 2.4 nm along two shift axes; -0.3 2.5 nm on specimen axes Rate = 12.58759 A/sec Drift is low enough after shot 2 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.08 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.513 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.48.27_batch_tomo_03_5_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 122.9 In SetExposureForMean 120, change by a factor of 0.9768 would require too small a change in exposure time Image shift -0.354 0.628 IS units -2050.1 366.7 unbinned pixels; need stage -0.063 -0.737 if reset Tilting to 0 Correcting tilt backlash -11.8 1.4 unbinned pixels; 1.8 3.7 nm along two shift axes; -0.1 -4.2 nm on specimen axes Rate = 20.78991 A/sec -5.8 2.0 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 10.72771 A/sec Drift is low enough after shot 2 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.17 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.12 nm/sec Defocus = -1.602 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.49.30_batch_tomo_03_5_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 122.3 In SetExposureForMean 120, change by a factor of 0.9813 would require too small a change in exposure time Image shift -0.375 0.680 IS units -2212.6 374.7 unbinned pixels; need stage -0.061 -0.794 if reset Tilting to -20 -20.00 degrees -4.5 -0.7 unbinned pixels; 0.3 1.6 nm along two shift axes; 0.4 -1.6 nm on specimen axes Rate = 8.01899 A/sec Drift is low enough after shot 1 Measured defocus = -3.65 microns changed by 0.15 to target drift = 0.03 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.466 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.50.29_batch_tomo_03_5_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 120.1 In SetExposureForMean 120, change by a factor of 0.9998 would require too small a change in exposure time Image shift -0.394 0.663 IS units -2184.6 440.1 unbinned pixels; need stage -0.084 -0.837 if reset Tilting to -22 15.4 -9.3 unbinned pixels; 4.9 4.0 nm along two shift axes; 2.8 5.7 nm on specimen axes Rate = 31.5848 A/sec 9.1 0.2 unbinned pixels; 1.0 3.0 nm along two shift axes; -0.3 3.2 nm on specimen axes Rate = 15.91285 A/sec 6.1 -0.4 unbinned pixels; 0.8 2.0 nm along two shift axes; -0.1 2.2 nm on specimen axes Rate = 10.7468 A/sec Drift is low enough after shot 3 Measured defocus = -3.58 microns changed by 0.08 to target drift = 0.12 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -0.381 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.51.42_batch_tomo_03_5_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0078 would require too small a change in exposure time Image shift -0.318 0.532 IS units -1754.7 360.2 unbinned pixels; need stage -0.070 -0.682 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 5.5 -5.0 unbinned pixels; 2.3 1.2 nm along two shift axes; 1.6 2.1 nm on specimen axes Rate = 13.13357 A/sec Drift is low enough after shot 1 Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.08 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -1.641 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.52.43_batch_tomo_03_5_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 122.2 In SetExposureForMean 120, change by a factor of 0.9820 would require too small a change in exposure time Image shift -0.356 0.627 IS units -2049.1 371.9 unbinned pixels; need stage -0.065 -0.737 if reset Tilting to 4 Correcting tilt backlash -23.8 3.3 unbinned pixels; 3.9 7.5 nm along two shift axes; -0.4 -8.4 nm on specimen axes Rate = 42.10678 A/sec -15.5 4.0 unbinned pixels; 3.1 4.7 nm along two shift axes; -0.9 -5.6 nm on specimen axes Rate = 28.07662 A/sec -11.8 2.9 unbinned pixels; 2.4 3.6 nm along two shift axes; -0.7 -4.2 nm on specimen axes Rate = 21.31664 A/sec -9.8 1.9 unbinned pixels; 1.8 3.0 nm along two shift axes; -0.4 -3.5 nm on specimen axes Rate = 17.53559 A/sec -8.1 2.3 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.5 -2.9 nm on specimen axes Rate = 14.78948 A/sec -7.1 1.8 unbinned pixels; 1.4 2.1 nm along two shift axes; -0.4 -2.6 nm on specimen axes Rate = 12.90085 A/sec Drift is low enough after shot 6 Measured defocus = -3.41 microns changed by -0.09 to target drift = 0.18 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.12 nm/sec Defocus = -1.731 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.54.09_batch_tomo_03_5_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 121.7 In SetExposureForMean 120, change by a factor of 0.9862 would require too small a change in exposure time Image shift -0.370 0.661 IS units -2153.9 377.2 unbinned pixels; need stage -0.064 -0.776 if reset Tilting to -24 -24.00 degrees -1.3 1.3 unbinned pixels; 0.6 0.3 nm along two shift axes; -0.4 -0.5 nm on specimen axes Rate = 3.18441 A/sec Drift is low enough after shot 1 Measured defocus = -3.64 microns changed by 0.14 to target drift = 0.02 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.236 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.55.08_batch_tomo_03_5_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0233 would require too small a change in exposure time Image shift -0.329 0.522 IS units -1738.7 399.7 unbinned pixels; need stage -0.084 -0.687 if reset Tilting to -26 13.4 -8.7 unbinned pixels; 4.5 3.4 nm along two shift axes; 2.6 5.0 nm on specimen axes Rate = 28.03031 A/sec 8.2 0.2 unbinned pixels; 0.9 2.7 nm along two shift axes; -0.3 2.9 nm on specimen axes Rate = 14.33351 A/sec 5.5 -0.1 unbinned pixels; 0.7 1.8 nm along two shift axes; -0.1 1.9 nm on specimen axes Rate = 9.6597 A/sec Drift is low enough after shot 3 Measured defocus = -3.57 microns changed by 0.07 to target drift = 0.12 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -0.167 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.56.16_batch_tomo_03_5_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, change by a factor of 1.0360 would require too small a change in exposure time Image shift -0.264 0.408 IS units -1365.9 330.9 unbinned pixels; need stage -0.072 -0.549 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 7.7 -4.7 unbinned pixels; 2.5 2.0 nm along two shift axes; 1.4 2.9 nm on specimen axes Rate = 15.86073 A/sec 4.0 4.0 unbinned pixels; 0.9 1.8 nm along two shift axes; -1.5 1.3 nm on specimen axes Rate = 10.01503 A/sec Drift is low enough after shot 2 Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.04 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.770 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.57.23_batch_tomo_03_5_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 121.2 In SetExposureForMean 120, change by a factor of 0.9905 would require too small a change in exposure time Image shift -0.323 0.559 IS units -1832.0 346.4 unbinned pixels; need stage -0.063 -0.663 if reset Tilting to 8 Correcting tilt backlash -2.1 -1.1 unbinned pixels; 0.1 0.8 nm along two shift axes; 0.5 -0.7 nm on specimen axes Rate = 4.12506 A/sec Drift is low enough after shot 1 Measured defocus = -3.41 microns changed by -0.09 to target drift = 0.08 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -1.860 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.58.21_batch_tomo_03_5_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 120.5 In SetExposureForMean 120, change by a factor of 0.9959 would require too small a change in exposure time Image shift -0.306 0.525 IS units -1722.6 334.1 unbinned pixels; need stage -0.062 -0.626 if reset Tilting to -28 -28.00 degrees -6.7 -4.7 unbinned pixels; 0.8 2.8 nm along two shift axes; 1.9 -2.2 nm on specimen axes Rate = 14.43533 A/sec 0.1 3.6 unbinned pixels; 1.2 0.5 nm along two shift axes; -1.3 -0.1 nm on specimen axes Rate = 6.41806 A/sec Drift is low enough after shot 2 Measured defocus = -3.59 microns changed by 0.09 to target drift = 0.07 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.071 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_18.59.26_batch_tomo_03_5_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 113.7 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift -0.242 0.321 IS units -1106.9 353.2 unbinned pixels; need stage -0.087 -0.456 if reset Tilting to -30 11.8 -7.9 unbinned pixels; 4.0 2.9 nm along two shift axes; 2.4 4.4 nm on specimen axes Rate = 24.86559 A/sec 7.7 0.9 unbinned pixels; 0.6 2.6 nm along two shift axes; -0.6 2.7 nm on specimen axes Rate = 13.54872 A/sec Drift is low enough after shot 2 Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.13 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -0.025 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.00.28_batch_tomo_03_5_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 121.0 In SetExposureForMean 120, change by a factor of 0.9912 would require too small a change in exposure time Image shift -0.179 0.207 IS units -738.4 291.1 unbinned pixels; need stage -0.077 -0.312 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -5.9 1.9 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 10.88339 A/sec Drift is low enough after shot 1 Measured defocus = -3.43 microns changed by -0.07 to target drift = 0.13 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -1.942 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.01.31_batch_tomo_03_5_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 129.8 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.307 0.500 IS units -1658.2 360.8 unbinned pixels; need stage -0.073 -0.607 if reset Tilting to 12 Correcting tilt backlash -10.6 6.2 unbinned pixels; 3.3 2.7 nm along two shift axes; -1.9 -3.9 nm on specimen axes Rate = 21.50466 A/sec -7.8 1.7 unbinned pixels; 1.5 2.4 nm along two shift axes; -0.3 -2.8 nm on specimen axes Rate = 13.93545 A/sec Drift is low enough after shot 2 Measured defocus = -3.40 microns changed by -0.10 to target drift = 0.08 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -2.050 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.02.34_batch_tomo_03_5_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 118.7 In SetExposureForMean 120, change by a factor of 1.0116 would require too small a change in exposure time Image shift -0.325 0.535 IS units -1768.6 376.7 unbinned pixels; need stage -0.075 -0.652 if reset Tilting to -32 -32.00 degrees -1.3 -0.5 unbinned pixels; 0.0 0.5 nm along two shift axes; 0.2 -0.4 nm on specimen axes Rate = 2.4913 A/sec Drift is low enough after shot 1 Measured defocus = -3.57 microns changed by 0.07 to target drift = 0.04 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = 0.048 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.03.35_batch_tomo_03_5_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 109.5 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift -0.171 0.139 IS units -543.5 332.8 unbinned pixels; need stage -0.098 -0.239 if reset Tilting to -34 8.1 -8.1 unbinned pixels; 3.6 1.7 nm along two shift axes; 2.6 3.1 nm on specimen axes Rate = 20.11325 A/sec 8.6 1.6 unbinned pixels; 0.5 3.0 nm along two shift axes; -0.8 3.0 nm on specimen axes Rate = 15.32183 A/sec 5.0 0.8 unbinned pixels; 0.3 1.8 nm along two shift axes; -0.4 1.7 nm on specimen axes Rate = 8.95849 A/sec Drift is low enough after shot 3 Measured defocus = -3.53 microns changed by 0.03 to target drift = 0.09 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = 0.079 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.04.43_batch_tomo_03_5_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, change by a factor of 1.0360 would require too small a change in exposure time Image shift -0.120 0.048 IS units -248.3 280.3 unbinned pixels; need stage -0.089 -0.117 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -9.1 3.3 unbinned pixels; 2.1 2.6 nm along two shift axes; -0.9 -3.3 nm on specimen axes Rate = 16.98762 A/sec -1.9 3.2 unbinned pixels; 1.3 0.3 nm along two shift axes; -1.1 -0.8 nm on specimen axes Rate = 6.51425 A/sec Drift is low enough after shot 2 Measured defocus = -3.40 microns changed by -0.10 to target drift = 0.09 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.160 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.05.51_batch_tomo_03_5_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 127.5 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.353 0.572 IS units -1897.3 417.4 unbinned pixels; need stage -0.085 -0.705 if reset Tilting to 16 Correcting tilt backlash -1.6 -1.8 unbinned pixels; 0.4 0.8 nm along two shift axes; 0.7 -0.5 nm on specimen axes Rate = 4.30147 A/sec Drift is low enough after shot 1 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.10 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -2.240 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.06.49_batch_tomo_03_5_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 116.4 In SetExposureForMean 120, change by a factor of 1.0324 would require too small a change in exposure time Image shift -0.320 0.485 IS units -1628.5 411.2 unbinned pixels; need stage -0.091 -0.613 if reset Tilting to -36 -36.00 degrees -1.2 -1.1 unbinned pixels; 0.2 0.5 nm along two shift axes; 0.4 -0.4 nm on specimen axes Rate = 2.89326 A/sec Drift is low enough after shot 1 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.07 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = 0.144 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.07.50_batch_tomo_03_5_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 105.6 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift -0.105 -0.024 IS units -37.9 309.4 unbinned pixels; need stage -0.105 -0.029 if reset Tilting to -38 5.2 -1.4 unbinned pixels; 1.1 1.5 nm along two shift axes; 0.3 1.9 nm on specimen axes Rate = 9.41881 A/sec Drift is low enough after shot 1 Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.07 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = 0.150 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.08.47_batch_tomo_03_5_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 120.3 In SetExposureForMean 120, change by a factor of 1.0037 would require too small a change in exposure time Image shift -0.081 -0.052 IS units 60.3 268.2 unbinned pixels; need stage -0.094 0.016 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -6.8 2.7 unbinned pixels; 1.7 1.9 nm along two shift axes; -0.7 -2.5 nm on specimen axes Rate = 12.75343 A/sec Drift is low enough after shot 1 Measured defocus = -3.37 microns changed by -0.13 to target drift = 0.11 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.379 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.09.50_batch_tomo_03_5_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 134.1 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift -0.330 0.499 IS units -1678.1 424.7 unbinned pixels; need stage -0.094 -0.638 if reset Tilting to 20 Correcting tilt backlash -7.4 -4.4 unbinned pixels; 0.6 3.0 nm along two shift axes; 1.8 -2.5 nm on specimen axes Rate = 15.10803 A/sec -1.9 3.7 unbinned pixels; 1.5 0.2 nm along two shift axes; -1.2 -0.8 nm on specimen axes Rate = 7.3951 A/sec Drift is low enough after shot 2 Measured defocus = -3.41 microns changed by -0.09 to target drift = 0.07 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -2.463 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.10.55_batch_tomo_03_5_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 122.7 In SetExposureForMean 120, change by a factor of 0.9798 would require too small a change in exposure time Image shift -0.278 0.409 IS units -1382.2 368.3 unbinned pixels; need stage -0.084 -0.533 if reset Tilting to -40 -40.00 degrees 3.1 0.4 unbinned pixels; 0.2 1.1 nm along two shift axes; -0.2 1.1 nm on specimen axes Rate = 5.46173 A/sec Drift is low enough after shot 1 Measured defocus = -3.58 microns changed by 0.08 to target drift = 0.05 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = 0.235 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.11.56_batch_tomo_03_5_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 103.4 In SetExposureForMean 120, exposure time changed to 1.6800 Trimming A by 35 40, reference by 20 20 Image shift -0.074 -0.088 IS units 165.4 284.8 unbinned pixels; need stage -0.103 0.064 if reset Tilting to -42 0.3 -7.9 unbinned pixels; 2.7 0.8 nm along two shift axes; 2.8 0.3 nm on specimen axes Rate = 13.93285 A/sec Drift is low enough after shot 1 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.03 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = 0.186 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.12.54_batch_tomo_03_5_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 101.9 In SetExposureForMean 120, exposure time changed to 1.8000 Trimming A by 80 80, reference by 35 40 Image shift -0.006 -0.206 IS units 552.2 213.1 unbinned pixels; need stage -0.090 0.253 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -22.5 1.9 unbinned pixels; 3.3 7.2 nm along two shift axes; 0.0 -7.9 nm on specimen axes Rate = 39.57281 A/sec -7.8 1.0 unbinned pixels; 1.3 2.5 nm along two shift axes; -0.1 -2.8 nm on specimen axes Rate = 13.8387 A/sec Drift is low enough after shot 2 Measured defocus = -3.37 microns changed by -0.13 to target drift = 0.15 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.11 nm/sec Defocus = -2.598 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.14.08_batch_tomo_03_5_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 139.4 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift -0.295 0.429 IS units -1451.6 395.6 unbinned pixels; need stage -0.091 -0.567 if reset Tilting to 24 Correcting tilt backlash -4.2 -6.4 unbinned pixels; 1.6 2.2 nm along two shift axes; 2.4 -1.3 nm on specimen axes Rate = 13.50646 A/sec Drift is low enough after shot 1 Measured defocus = -3.43 microns changed by -0.07 to target drift = 0.08 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = -2.675 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.15.13_batch_tomo_03_5_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 119.0 In SetExposureForMean 120, change by a factor of 1.0127 would require too small a change in exposure time Image shift -0.233 0.269 IS units -959.6 378.9 unbinned pixels; need stage -0.101 -0.385 if reset Tilting to -44 -44.00 degrees 3.8 0.1 unbinned pixels; 0.4 1.3 nm along two shift axes; -0.1 1.3 nm on specimen axes Rate = 6.71511 A/sec Drift is low enough after shot 1 Measured defocus = -3.63 microns changed by 0.13 to target drift = 0.09 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = 0.322 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.16.14_batch_tomo_03_5_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 78.5 In SetExposureForMean 120, exposure time changed to 2.1600 Trimming A by 140 140, reference by 85 85 Image shift 0.097 -0.392 IS units 1158.0 109.3 unbinned pixels; need stage -0.073 0.564 if reset Tilting to -46 2.4 -2.5 unbinned pixels; 1.1 0.5 nm along two shift axes; 0.8 0.9 nm on specimen axes Rate = 6.14103 A/sec Drift is low enough after shot 1 Measured defocus = -3.38 microns changed by -0.12 to target drift = 0.11 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = 0.209 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.17.11_batch_tomo_03_5_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 104.9 In SetExposureForMean 120, exposure time changed to 2.2800 Trimming A by 125 125, reference by 140 140 Image shift 0.176 -0.528 IS units 1603.4 24.6 unbinned pixels; need stage -0.058 0.815 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -14.6 1.3 unbinned pixels; 2.1 4.7 nm along two shift axes; -0.0 -5.2 nm on specimen axes Rate = 25.79258 A/sec -9.2 2.5 unbinned pixels; 1.9 2.8 nm along two shift axes; -0.6 -3.3 nm on specimen axes Rate = 16.68575 A/sec -6.3 1.6 unbinned pixels; 1.3 1.9 nm along two shift axes; -0.4 -2.3 nm on specimen axes Rate = 11.4427 A/sec Drift is low enough after shot 3 Measured defocus = -3.34 microns changed by -0.16 to target drift = 0.08 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -2.828 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.18.27_batch_tomo_03_5_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 170.8 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift -0.236 0.307 IS units -1063.2 350.7 unbinned pixels; need stage -0.088 -0.431 if reset Tilting to 28 Correcting tilt backlash -3.3 -4.9 unbinned pixels; 1.2 1.7 nm along two shift axes; 1.8 -1.0 nm on specimen axes Rate = 10.40515 A/sec Drift is low enough after shot 1 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.08 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -2.902 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.19.26_batch_tomo_03_5_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 114.5 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift -0.183 0.192 IS units -700.9 315.1 unbinned pixels; need stage -0.087 -0.292 if reset Tilting to -48 -48.00 degrees 5.1 -4.7 unbinned pixels; 2.1 1.1 nm along two shift axes; 1.5 1.9 nm on specimen axes Rate = 12.04621 A/sec Drift is low enough after shot 1 Measured defocus = -3.45 microns changed by -0.05 to target drift = 0.10 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = 0.151 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.20.28_batch_tomo_03_5_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 38.0 In SetExposureForMean 120, exposure time changed to 6.4800 Trimming A by 20 20, reference by 125 130 Image shift 0.644 -1.297 IS units 4146.9 -518.6 unbinned pixels; need stage 0.051 2.216 if reset Tilting to -50 8.9 -1.4 unbinned pixels; 1.5 2.8 nm along two shift axes; 0.2 3.2 nm on specimen axes Rate = 15.86705 A/sec 5.6 -0.6 unbinned pixels; 0.8 1.8 nm along two shift axes; 0.0 2.0 nm on specimen axes Rate = 9.85161 A/sec Drift is low enough after shot 2 Measured defocus = -2.87 microns changed by -0.63 to target drift = 0.19 nm/sec Measured defocus = -3.45 microns changed by -0.05 to target drift = 0.10 nm/sec Defocus = -0.524 um Dewars are NOT busy filling 54 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.21.30_batch_tomo_03_5_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 237.1 In SetExposureForMean 120, exposure time changed to 3.4800 Image shift 0.653 -1.650 IS units 5112.3 -207.7 unbinned pixels; need stage -0.086 2.823 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -13.8 2.6 unbinned pixels; 2.5 4.3 nm along two shift axes; -0.5 -4.9 nm on specimen axes Rate = 24.65318 A/sec -7.4 1.6 unbinned pixels; 1.4 2.3 nm along two shift axes; -0.3 -2.6 nm on specimen axes Rate = 13.24967 A/sec Drift is low enough after shot 2 Measured defocus = -3.37 microns changed by -0.13 to target drift = 0.13 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -3.029 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.22.45_batch_tomo_03_5_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 240.2 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift -0.200 0.220 IS units -794.2 335.6 unbinned pixels; need stage -0.091 -0.337 if reset Tilting to 32 Correcting tilt backlash -4.6 -6.6 unbinned pixels; 1.7 2.3 nm along two shift axes; 2.5 -1.4 nm on specimen axes Rate = 14.20198 A/sec -0.8 2.3 unbinned pixels; 0.9 0.0 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.2673 A/sec Drift is low enough after shot 2 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.06 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -3.109 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.23.51_batch_tomo_03_5_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 113.7 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift -0.162 0.133 IS units -519.1 313.7 unbinned pixels; need stage -0.092 -0.228 if reset Tilting to -52 -52.00 degrees 10.1 1.3 unbinned pixels; 0.7 3.5 nm along two shift axes; -0.8 3.5 nm on specimen axes Rate = 17.88342 A/sec 0.2 -0.8 unbinned pixels; 0.3 0.0 nm along two shift axes; 0.3 0.1 nm on specimen axes Rate = 1.41525 A/sec Drift is low enough after shot 2 Measured defocus = -3.26 microns changed by -0.24 to target drift = 0.10 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.14 nm/sec Defocus = -0.777 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.24.59_batch_tomo_03_5_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 60.1 In SetExposureForMean 120, exposure time changed to 3.4800 Trimming A by 180 180, reference by 20 20 Image shift 1.053 -1.666 IS units 5550.3 -1280.8 unbinned pixels; need stage 0.270 3.255 if reset Tilting to -54 2.6 -1.1 unbinned pixels; 0.7 0.7 nm along two shift axes; 0.3 1.0 nm on specimen axes Rate = 4.96953 A/sec Drift is low enough after shot 1 Measured defocus = -3.39 microns changed by -0.11 to target drift = 0.22 nm/sec Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.13 nm/sec Defocus = -0.943 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.25.56_batch_tomo_03_5_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 124.3 In SetExposureForMean 120, exposure time changed to 3.2400 Trimming A by 20 70, reference by 180 180 Image shift 1.194 -1.738 IS units 5885.9 -1597.3 unbinned pixels; need stage 0.369 3.629 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -8.9 -6.4 unbinned pixels; 1.1 3.7 nm along two shift axes; 2.5 -2.9 nm on specimen axes Rate = 19.31134 A/sec -3.2 4.4 unbinned pixels; 1.8 0.5 nm along two shift axes; -1.4 -1.3 nm on specimen axes Rate = 9.5411 A/sec Drift is low enough after shot 2 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.07 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -3.199 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.27.08_batch_tomo_03_5_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 212.9 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift -0.134 0.083 IS units -358.0 286.8 unbinned pixels; need stage -0.088 -0.164 if reset Tilting to 36 Correcting tilt backlash -6.7 -3.0 unbinned pixels; 0.2 2.6 nm along two shift axes; 1.2 -2.3 nm on specimen axes Rate = 12.89133 A/sec Drift is low enough after shot 1 Measured defocus = -3.36 microns changed by -0.14 to target drift = 0.07 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -3.331 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.28.08_batch_tomo_03_5_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 115.2 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift -0.115 0.048 IS units -242.8 267.6 unbinned pixels; need stage -0.085 -0.117 if reset Tilting to -56 -56.00 degrees 17.4 3.4 unbinned pixels; 0.9 6.2 nm along two shift axes; -1.7 6.0 nm on specimen axes Rate = 31.11577 A/sec 5.7 0.6 unbinned pixels; 0.5 2.0 nm along two shift axes; -0.4 2.0 nm on specimen axes Rate = 10.07073 A/sec Drift is low enough after shot 2 Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.07 nm/sec Measured defocus = -3.45 microns changed by -0.05 to target drift = 0.12 nm/sec Defocus = -0.955 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.29.17_batch_tomo_03_5_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 65.5 In SetExposureForMean 120, exposure time changed to 3.4800 Trimming A by 20 20, reference by 20 70 Image shift 1.215 -1.783 IS units 6027.1 -1610.9 unbinned pixels; need stage 0.369 3.904 if reset Tilting to -58 3.0 -0.8 unbinned pixels; 0.6 0.9 nm along two shift axes; 0.2 1.1 nm on specimen axes Rate = 5.3791 A/sec Drift is low enough after shot 1 Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.09 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.04 nm/sec Defocus = -1.008 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.30.14_batch_tomo_03_5_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 108.3 In SetExposureForMean 120, exposure time changed to 3.9600 Image shift 1.236 -1.830 IS units 6175.0 -1623.2 unbinned pixels; need stage 0.369 4.220 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -17.5 -0.3 unbinned pixels; 1.9 5.8 nm along two shift axes; 0.7 -6.1 nm on specimen axes Rate = 30.77642 A/sec -8.4 6.2 unbinned pixels; 3.0 2.0 nm along two shift axes; -1.9 -3.1 nm on specimen axes Rate = 18.25822 A/sec -5.0 2.7 unbinned pixels; 1.5 1.3 nm along two shift axes; -0.8 -1.8 nm on specimen axes Rate = 9.97198 A/sec Drift is low enough after shot 3 Measured defocus = -3.40 microns changed by -0.10 to target drift = 0.12 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -3.433 um Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.31.32_batch_tomo_03_5_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 245.7 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift -0.108 0.019 IS units -156.7 276.2 unbinned pixels; need stage -0.090 -0.082 if reset Tilting to 40 Correcting tilt backlash -6.6 -8.3 unbinned pixels; 2.0 3.2 nm along two shift axes; 3.1 -2.1 nm on specimen axes Rate = 18.72411 A/sec -1.8 0.8 unbinned pixels; 0.5 0.5 nm along two shift axes; -0.2 -0.6 nm on specimen axes Rate = 3.39936 A/sec Drift is low enough after shot 2 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.02 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -3.502 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.32.39_batch_tomo_03_5_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 115.0 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift -0.059 -0.097 IS units 203.9 254.1 unbinned pixels; need stage -0.094 0.083 if reset Tilting to -60 -60.00 degrees 18.0 -7.4 unbinned pixels; 4.6 5.1 nm along two shift axes; 2.0 6.5 nm on specimen axes Rate = 34.11998 A/sec 6.1 0.9 unbinned pixels; 0.4 2.1 nm along two shift axes; -0.5 2.1 nm on specimen axes Rate = 10.80945 A/sec Drift is low enough after shot 2 Measured defocus = -3.40 microns changed by -0.10 to target drift = 0.06 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.14 nm/sec Defocus = -1.106 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.33.49_batch_tomo_03_5_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 53.9 In SetExposureForMean 120, exposure time changed to 4.8000 Trimming A by 35 35, reference by 20 20 Image shift 1.378 -2.034 IS units 6866.4 -1815.1 unbinned pixels; need stage 0.413 4.973 if reset Tilting to -62 4.9 3.1 unbinned pixels; 0.5 2.0 nm along two shift axes; -1.2 1.6 nm on specimen axes Rate = 10.22108 A/sec Drift is low enough after shot 1 Measured defocus = -3.34 microns changed by -0.16 to target drift = 0.03 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -1.251 um Dewars are NOT busy filling 40 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.34.46_batch_tomo_03_5_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 114.5 In SetExposureForMean 120, exposure time changed to 5.4000 Trimming A by 40 40, reference by 45 45 Image shift 1.419 -2.116 IS units 7130.4 -1849.1 unbinned pixels; need stage 0.417 5.498 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -16.1 -2.5 unbinned pixels; 1.0 5.6 nm along two shift axes; 1.4 -5.6 nm on specimen axes Rate = 28.64917 A/sec -3.7 6.0 unbinned pixels; 2.4 0.5 nm along two shift axes; -2.0 -1.5 nm on specimen axes Rate = 12.34363 A/sec Drift is low enough after shot 2 Measured defocus = -3.41 microns changed by -0.09 to target drift = 0.10 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -3.594 um Dewars are NOT busy filling 45 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.36.05_batch_tomo_03_5_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 312.4 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift -0.024 -0.150 IS units 381.7 207.6 unbinned pixels; need stage -0.083 0.173 if reset Tilting to 44 Correcting tilt backlash -4.6 -5.9 unbinned pixels; 1.4 2.2 nm along two shift axes; 2.2 -1.4 nm on specimen axes Rate = 13.13932 A/sec Drift is low enough after shot 1 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.07 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -3.649 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.37.07_batch_tomo_03_5_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 113.3 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.021 -0.237 IS units 663.0 169.9 unbinned pixels; need stage -0.079 0.318 if reset Tilting to -64 -64.00 degrees 17.3 0.1 unbinned pixels; 2.0 5.7 nm along two shift axes; -0.6 6.1 nm on specimen axes Rate = 30.3597 A/sec 8.9 -0.4 unbinned pixels; 1.2 2.9 nm along two shift axes; -0.1 3.1 nm on specimen axes Rate = 15.6143 A/sec 3.7 -0.1 unbinned pixels; 0.5 1.2 nm along two shift axes; -0.1 1.3 nm on specimen axes Rate = 6.57568 A/sec Drift is low enough after shot 3 Measured defocus = -3.30 microns changed by -0.20 to target drift = 0.17 nm/sec Measured defocus = -3.55 microns changed by 0.05 to target drift = 0.12 nm/sec Defocus = -1.402 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.38.22_batch_tomo_03_5_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 44.3 In SetExposureForMean 120, exposure time changed to 6.4800 Trimming A by 20 20, reference by 50 50 Image shift 1.512 -2.221 IS units 7507.6 -2002.3 unbinned pixels; need stage 0.458 6.204 if reset Tilting to -66 -3.9 -0.6 unbinned pixels; 0.3 1.4 nm along two shift axes; 0.3 -1.3 nm on specimen axes Rate = 6.93062 A/sec Drift is low enough after shot 1 Measured defocus = -3.17 microns changed by -0.33 to target drift = 0.05 nm/sec Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.01 nm/sec Defocus = -1.788 um Dewars are NOT busy filling 54 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.39.20_batch_tomo_03_5_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, exposure time changed to 7.4400 Image shift 1.575 -2.333 IS units 7872.2 -2068.0 unbinned pixels; need stage 0.469 7.008 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -14.3 0.6 unbinned pixels; 1.9 4.7 nm along two shift axes; 0.2 -5.0 nm on specimen axes Rate = 25.17033 A/sec -5.8 4.0 unbinned pixels; 2.0 1.4 nm along two shift axes; -1.2 -2.2 nm on specimen axes Rate = 12.3806 A/sec Drift is low enough after shot 2 Measured defocus = -3.32 microns changed by -0.18 to target drift = 0.06 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -3.834 um Dewars are NOT busy filling 62 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.40.35_batch_tomo_03_5_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 397.9 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift -0.014 -0.169 IS units 443.8 200.3 unbinned pixels; need stage -0.083 0.217 if reset Tilting to 48 Correcting tilt backlash 1.1 -17.1 unbinned pixels; 5.8 1.7 nm along two shift axes; 6.0 0.9 nm on specimen axes Rate = 30.26338 A/sec 1.8 0.3 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.2 0.6 nm on specimen axes Rate = 3.1336 A/sec Drift is low enough after shot 2 Measured defocus = -3.63 microns changed by 0.13 to target drift = 0.05 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -3.696 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.41.46_batch_tomo_03_5_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.087 -0.398 IS units 1163.8 141.4 unbinned pixels; need stage -0.084 0.608 if reset Tilting to -68 -68.00 degrees 15.4 -0.3 unbinned pixels; 1.9 5.1 nm along two shift axes; -0.4 5.4 nm on specimen axes Rate = 27.00397 A/sec -9.2 0.2 unbinned pixels; 1.1 3.0 nm along two shift axes; 0.2 -3.2 nm on specimen axes Rate = 16.21374 A/sec 1.9 0.5 unbinned pixels; 0.0 0.7 nm along two shift axes; -0.2 0.7 nm on specimen axes Rate = 3.47032 A/sec Drift is low enough after shot 3 Measured defocus = -3.39 microns changed by -0.11 to target drift = 0.14 nm/sec Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.02 nm/sec Defocus = -1.977 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.43.06_batch_tomo_03_5_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 36.5 In SetExposureForMean 120, exposure time changed to 8.6400 Image shift 1.670 -2.445 IS units 8268.0 -2220.0 unbinned pixels; need stage 0.510 7.996 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 0.54 microns changed by -4.04 to target drift = 0.58 nm/sec Focus area changed from stored state, axis position -2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_04.st Running script 1 at item 7 (-388.63, -264.69) 19:44:02 Refine ZLP: additional offset = -1.0 eV; total offset = -11.8 eV Item 7: Stage: -388.63 -264.69 -223 Label: 7 Note: Sec 6 - anchor_maps.map Target defocus is: -3.50 um Target defocus is: -3.75 um Target defocus = -3.75 Trimming A by 22 130, reference by 21 130 Angle = -4.40, increment = 0.60, shift = -3.2 -6.4 pixels, -0.009 um Y Trimming A by 22 130, reference by 21 130 Angle = -3.20, increment = 1.80, shift = -59.2 -15.6 pixels, -0.138 um Y Trimming A by 21 130, reference by 21 130 Angle = 0.40, increment = 5.41, shift = -171.0 -37.1 pixels, -0.397 um Y Trimming A by 21 130, reference by 21 130 Angle = 3.00, increment = 8.00, shift = -238.9 -48.6 pixels, -0.554 um Y Rough eucentricity: changing Z by 3.98 to -219.40, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 24 135, reference by 24 135 Angle = 18.00, shift = -38.8, 1.7 pixels, 0.014, -0.088 um, IS = -0.014, 0.088 Trimming A by 34 130, reference by 24 135 Angle = 11.00, shift = -81.3, -6.7 pixels, 0.006, -0.186 um, IS = -0.019, 0.274 Trimming A by 29 130, reference by 34 130 Angle = 4.00, shift = -52.7, -3.4 pixels, 0.006, -0.120 um, IS = -0.025, 0.394 Trimming A by 23 23 20 130, reference by 26 21 20 130 Angle = -4.00, shift = -11.0, -18.1 pixels, -0.038, -0.030 um, IS = 0.013, 0.424 Trimming A by 21 130, reference by 23 130 Angle = -11.00, shift = 23.9, -2.9 pixels, -0.013, 0.053 um, IS = 0.026, 0.370 Trimming A by 26 130, reference by 21 130 Angle = -18.00, shift = 29.5, 11.5 pixels, 0.018, 0.070 um, IS = 0.007, 0.300 Trimming A by 26 130, reference by 25 130 Angle = -24.00, shift = 56.6, 12.1 pixels, 0.013, 0.132 um, IS = -0.006, 0.169 General solution delZ 0.26, delY 3.42, yZero 0.40 solution with assumed YZero 0.41: delZ 0.26, delY 3.52 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.26 microns and lateral displacement of -3.42 microns Trimming A by 23 24 20 140, reference by 21 22 20 135 Disparity in stage position after aligning to center of map frame: -0.01 -1.07 Trimming A by 25 22 20 135, reference by 21 22 20 135 Disparity in stage position after aligning to target position: 0.39 -1.49 (2nd move error 0.03 0.12) Disparity in stage position after aligning to map item itself: 0.01 -0.96 (align shift -0.02 -0.03) Finished aligning to item in 3 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -388.63 -265.73 -219.64 1.0 -3.3 unbinned pixels; 1.2 0.1 nm along two shift axes; 1.1 0.5 nm on specimen axes Rate = 6.06528 A/sec Drift is low enough after shot 1 Measured defocus = 1.36 microns changed by -5.11 to target drift = 0.13 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.02 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -1.216 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.49.02_batch_tomo_04_7_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 100.8 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.044 -0.070 IS units 233.7 -52.8 unbinned pixels; need stage 0.011 0.086 if reset Tilting to -8 Correcting tilt backlash 12.0 0.7 unbinned pixels; 1.2 4.0 nm along two shift axes; -0.6 4.2 nm on specimen axes Rate = 21.06069 A/sec 6.1 0.5 unbinned pixels; 0.5 2.1 nm along two shift axes; -0.4 2.1 nm on specimen axes Rate = 10.71483 A/sec Drift is low enough after shot 2 Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.10 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.301 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.50.06_batch_tomo_04_7_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 121.3 In SetExposureForMean 120, change by a factor of 0.9895 would require too small a change in exposure time Image shift 0.005 -0.070 IS units 193.4 53.4 unbinned pixels; need stage -0.024 0.067 if reset Tilting to -12 -12.00 degrees -8.2 1.2 unbinned pixels; 1.3 2.6 nm along two shift axes; -0.2 -2.9 nm on specimen axes Rate = 14.44906 A/sec -5.2 5.0 unbinned pixels; 2.3 1.1 nm along two shift axes; -1.6 -2.0 nm on specimen axes Rate = 12.64161 A/sec Drift is low enough after shot 2 Measured defocus = -3.85 microns changed by 0.10 to target drift = 0.10 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -1.106 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.51.09_batch_tomo_04_7_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 121.0 In SetExposureForMean 120, change by a factor of 0.9922 would require too small a change in exposure time Image shift -0.037 0.002 IS units -42.1 99.8 unbinned pixels; need stage -0.033 -0.019 if reset Tilting to -14 7.9 -0.7 unbinned pixels; 1.1 2.5 nm along two shift axes; -0.0 2.8 nm on specimen axes Rate = 13.92686 A/sec Drift is low enough after shot 1 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.14 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.08 nm/sec Defocus = -1.015 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.52.06_batch_tomo_04_7_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 120.5 In SetExposureForMean 120, change by a factor of 0.9962 would require too small a change in exposure time Image shift -0.069 0.059 IS units -227.8 132.8 unbinned pixels; need stage -0.039 -0.087 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 9.8 -2.2 unbinned pixels; 1.9 3.0 nm along two shift axes; 0.5 3.5 nm on specimen axes Rate = 17.53583 A/sec 8.6 -0.6 unbinned pixels; 1.2 2.8 nm along two shift axes; -0.0 3.0 nm on specimen axes Rate = 15.11098 A/sec 5.5 -0.9 unbinned pixels; 0.9 1.7 nm along two shift axes; 0.2 1.9 nm on specimen axes Rate = 9.72737 A/sec Drift is low enough after shot 3 Measured defocus = -3.66 microns changed by -0.09 to target drift = 0.08 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -1.402 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.53.16_batch_tomo_04_7_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 121.1 In SetExposureForMean 120, change by a factor of 0.9912 would require too small a change in exposure time Image shift 0.025 -0.128 IS units 370.6 52.0 unbinned pixels; need stage -0.029 0.130 if reset Tilting to -4 Correcting tilt backlash -7.5 1.2 unbinned pixels; 1.3 2.3 nm along two shift axes; -0.2 -2.7 nm on specimen axes Rate = 13.26644 A/sec Drift is low enough after shot 1 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.09 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -1.442 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.54.14_batch_tomo_04_7_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 120.9 In SetExposureForMean 120, change by a factor of 0.9925 would require too small a change in exposure time Image shift 0.007 -0.080 IS units 223.7 58.6 unbinned pixels; need stage -0.027 0.077 if reset Tilting to -16 -16.00 degrees -4.7 0.8 unbinned pixels; 0.8 1.4 nm along two shift axes; -0.1 -1.7 nm on specimen axes Rate = 8.28144 A/sec Drift is low enough after shot 1 Measured defocus = -3.87 microns changed by 0.12 to target drift = 0.07 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.887 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.55.13_batch_tomo_04_7_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 120.2 In SetExposureForMean 120, change by a factor of 0.9988 would require too small a change in exposure time Image shift -0.078 0.047 IS units -203.8 168.3 unbinned pixels; need stage -0.052 -0.081 if reset Tilting to -18 12.3 -11.2 unbinned pixels; 5.2 2.7 nm along two shift axes; 3.6 4.6 nm on specimen axes Rate = 29.1499 A/sec 7.4 1.1 unbinned pixels; 0.5 2.6 nm along two shift axes; -0.6 2.6 nm on specimen axes Rate = 13.09076 A/sec Drift is low enough after shot 2 Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.14 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -0.853 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.56.15_batch_tomo_04_7_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 119.6 In SetExposureForMean 120, change by a factor of 1.0039 would require too small a change in exposure time Image shift -0.003 -0.093 IS units 249.5 95.9 unbinned pixels; need stage -0.041 0.089 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 9.7 -2.3 unbinned pixels; 1.9 2.9 nm along two shift axes; 0.5 3.5 nm on specimen axes Rate = 17.52358 A/sec 8.7 -0.4 unbinned pixels; 1.2 2.8 nm along two shift axes; -0.1 3.1 nm on specimen axes Rate = 15.26924 A/sec 4.3 -0.8 unbinned pixels; 0.8 1.3 nm along two shift axes; 0.2 1.5 nm on specimen axes Rate = 7.6022 A/sec Drift is low enough after shot 3 Measured defocus = -3.69 microns changed by -0.06 to target drift = 0.07 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -1.508 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.57.27_batch_tomo_04_7_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 120.6 In SetExposureForMean 120, change by a factor of 0.9946 would require too small a change in exposure time Image shift 0.023 -0.134 IS units 386.3 64.1 unbinned pixels; need stage -0.034 0.135 if reset Tilting to 0 Correcting tilt backlash -24.4 3.1 unbinned pixels; 3.9 7.7 nm along two shift axes; -0.3 -8.6 nm on specimen axes Rate = 43.11402 A/sec -7.3 4.7 unbinned pixels; 2.4 1.8 nm along two shift axes; -1.4 -2.7 nm on specimen axes Rate = 15.21754 A/sec -5.5 1.1 unbinned pixels; 1.0 1.7 nm along two shift axes; -0.2 -2.0 nm on specimen axes Rate = 9.88008 A/sec Drift is low enough after shot 3 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.17 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.15 nm/sec Defocus = -1.566 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.58.42_batch_tomo_04_7_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 120.1 In SetExposureForMean 120, change by a factor of 0.9988 would require too small a change in exposure time Image shift -0.020 -0.037 IS units 79.8 89.3 unbinned pixels; need stage -0.033 0.025 if reset Tilting to -20 -20.00 degrees -2.0 0.6 unbinned pixels; 0.4 0.6 nm along two shift axes; -0.1 -0.7 nm on specimen axes Rate = 3.60092 A/sec Drift is low enough after shot 1 Measured defocus = -3.87 microns changed by 0.12 to target drift = 0.03 nm/sec Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.02 nm/sec Defocus = -0.730 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_19.59.41_batch_tomo_04_7_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 118.9 In SetExposureForMean 120, change by a factor of 1.0102 would require too small a change in exposure time Image shift -0.017 -0.091 IS units 228.9 132.7 unbinned pixels; need stage -0.053 0.082 if reset Tilting to -22 13.1 -9.7 unbinned pixels; 4.7 3.2 nm along two shift axes; 3.0 4.9 nm on specimen axes Rate = 28.56655 A/sec 8.6 0.3 unbinned pixels; 0.9 2.9 nm along two shift axes; -0.4 3.0 nm on specimen axes Rate = 15.08969 A/sec 5.2 -0.5 unbinned pixels; 0.8 1.6 nm along two shift axes; 0.0 1.8 nm on specimen axes Rate = 9.12953 A/sec Drift is low enough after shot 3 Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.12 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -0.710 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.00.48_batch_tomo_04_7_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 118.3 In SetExposureForMean 120, change by a factor of 1.0160 would require too small a change in exposure time Image shift 0.051 -0.210 IS units 617.8 61.4 unbinned pixels; need stage -0.040 0.233 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 6.3 -1.6 unbinned pixels; 1.3 1.9 nm along two shift axes; 0.4 2.3 nm on specimen axes Rate = 11.43879 A/sec Drift is low enough after shot 1 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.12 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -1.599 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.01.49_batch_tomo_04_7_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 119.6 In SetExposureForMean 120, change by a factor of 1.0034 would require too small a change in exposure time Image shift -0.008 -0.072 IS units 187.4 90.2 unbinned pixels; need stage -0.037 0.063 if reset Tilting to 4 Correcting tilt backlash -29.8 6.9 unbinned pixels; 5.8 9.1 nm along two shift axes; -1.5 -10.7 nm on specimen axes Rate = 53.67307 A/sec -16.0 4.9 unbinned pixels; 3.5 4.7 nm along two shift axes; -1.2 -5.8 nm on specimen axes Rate = 29.3735 A/sec -13.4 3.7 unbinned pixels; 2.8 4.0 nm along two shift axes; -0.9 -4.8 nm on specimen axes Rate = 24.40841 A/sec -20.3 5.4 unbinned pixels; 4.1 6.1 nm along two shift axes; -1.3 -7.3 nm on specimen axes Rate = 36.75577 A/sec -8.6 0.5 unbinned pixels; 1.2 2.8 nm along two shift axes; 0.1 -3.0 nm on specimen axes Rate = 15.08865 A/sec -4.3 0.2 unbinned pixels; 0.6 1.4 nm along two shift axes; 0.1 -1.5 nm on specimen axes Rate = 7.4698 A/sec Drift is low enough after shot 6 Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.14 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.15 nm/sec Defocus = -1.669 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.03.20_batch_tomo_04_7_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 119.1 In SetExposureForMean 120, change by a factor of 1.0073 would require too small a change in exposure time Image shift -0.037 -0.008 IS units -14.7 108.3 unbinned pixels; need stage -0.037 -0.009 if reset Tilting to -24 -24.00 degrees -2.9 -0.3 unbinned pixels; 0.2 1.0 nm along two shift axes; 0.2 -1.0 nm on specimen axes Rate = 5.16024 A/sec Drift is low enough after shot 1 Measured defocus = -3.86 microns changed by 0.11 to target drift = 0.02 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.590 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.04.20_batch_tomo_04_7_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 117.3 In SetExposureForMean 120, change by a factor of 1.0241 would require too small a change in exposure time Image shift 0.020 -0.182 IS units 512.6 118.0 unbinned pixels; need stage -0.056 0.194 if reset Tilting to -26 13.2 -9.2 unbinned pixels; 4.6 3.3 nm along two shift axes; 2.8 4.9 nm on specimen axes Rate = 28.23603 A/sec 9.0 0.5 unbinned pixels; 0.9 3.0 nm along two shift axes; -0.5 3.1 nm on specimen axes Rate = 15.7834 A/sec 5.5 0.0 unbinned pixels; 0.6 1.8 nm along two shift axes; -0.2 1.9 nm on specimen axes Rate = 9.7112 A/sec Drift is low enough after shot 3 Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.10 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -0.604 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.05.29_batch_tomo_04_7_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, change by a factor of 1.0332 would require too small a change in exposure time Image shift 0.085 -0.293 IS units 878.3 47.8 unbinned pixels; need stage -0.043 0.344 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 8.7 -4.3 unbinned pixels; 2.4 2.4 nm along two shift axes; 1.2 3.2 nm on specimen axes Rate = 16.95377 A/sec 8.1 2.9 unbinned pixels; 0.0 3.0 nm along two shift axes; -1.3 2.8 nm on specimen axes Rate = 15.1843 A/sec 4.0 1.7 unbinned pixels; 0.1 1.5 nm along two shift axes; -0.7 1.3 nm on specimen axes Rate = 7.64351 A/sec Drift is low enough after shot 3 Measured defocus = -3.70 microns changed by -0.05 to target drift = 0.06 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.717 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.06.41_batch_tomo_04_7_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 118.3 In SetExposureForMean 120, change by a factor of 1.0144 would require too small a change in exposure time Image shift -0.006 -0.095 IS units 250.8 105.6 unbinned pixels; need stage -0.044 0.086 if reset Tilting to 8 Correcting tilt backlash -36.0 10.1 unbinned pixels; 7.5 10.7 nm along two shift axes; -2.4 -12.9 nm on specimen axes Rate = 65.46154 A/sec -17.3 5.7 unbinned pixels; 3.9 5.0 nm along two shift axes; -1.5 -6.2 nm on specimen axes Rate = 31.88652 A/sec -11.0 2.7 unbinned pixels; 2.2 3.3 nm along two shift axes; -0.6 -3.9 nm on specimen axes Rate = 19.77938 A/sec -8.0 0.9 unbinned pixels; 1.3 2.5 nm along two shift axes; -0.1 -2.8 nm on specimen axes Rate = 14.18469 A/sec -5.4 3.1 unbinned pixels; 1.7 1.4 nm along two shift axes; -0.9 -2.0 nm on specimen axes Rate = 10.95892 A/sec Drift is low enough after shot 5 Measured defocus = -3.66 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -1.790 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.08.01_batch_tomo_04_7_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0211 would require too small a change in exposure time Image shift -0.039 -0.024 IS units 26.0 128.3 unbinned pixels; need stage -0.045 0.005 if reset Tilting to -28 -28.00 degrees 0.6 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.72778 A/sec Drift is low enough after shot 1 Measured defocus = -3.89 microns changed by 0.14 to target drift = 0.05 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.461 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.09.02_batch_tomo_04_7_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 115.3 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.052 -0.273 IS units 790.3 118.3 unbinned pixels; need stage -0.065 0.312 if reset Tilting to -30 11.0 -2.0 unbinned pixels; 1.9 3.4 nm along two shift axes; 0.3 3.9 nm on specimen axes Rate = 19.65664 A/sec 7.7 -1.6 unbinned pixels; 1.4 2.4 nm along two shift axes; 0.3 2.8 nm on specimen axes Rate = 13.78818 A/sec Drift is low enough after shot 2 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.13 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -0.500 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.10.05_batch_tomo_04_7_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 123.6 In SetExposureForMean 120, change by a factor of 0.9725 would require too small a change in exposure time Image shift 0.071 -0.290 IS units 855.6 83.3 unbinned pixels; need stage -0.055 0.346 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -4.6 -2.1 unbinned pixels; 0.2 1.8 nm along two shift axes; 0.9 -1.5 nm on specimen axes Rate = 8.85131 A/sec Drift is low enough after shot 1 Measured defocus = -3.66 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -1.865 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.11.06_batch_tomo_04_7_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 126.4 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.018 -0.100 IS units 253.4 145.4 unbinned pixels; need stage -0.058 0.086 if reset Tilting to 12 Correcting tilt backlash -7.7 -2.2 unbinned pixels; 0.2 2.8 nm along two shift axes; 1.0 -2.6 nm on specimen axes Rate = 14.17167 A/sec -0.1 3.6 unbinned pixels; 1.2 0.4 nm along two shift axes; -1.3 -0.1 nm on specimen axes Rate = 6.3519 A/sec Drift is low enough after shot 2 Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.08 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -1.887 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.12.10_batch_tomo_04_7_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 115.6 In SetExposureForMean 120, change by a factor of 1.0380 would require too small a change in exposure time Image shift 0.027 -0.180 IS units 514.2 98.8 unbinned pixels; need stage -0.050 0.183 if reset Tilting to -32 -32.00 degrees -1.2 -0.6 unbinned pixels; 0.0 0.5 nm along two shift axes; 0.2 -0.4 nm on specimen axes Rate = 2.40037 A/sec Drift is low enough after shot 1 Measured defocus = -3.83 microns changed by 0.08 to target drift = 0.04 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.410 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.13.11_batch_tomo_04_7_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 112.7 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.062 -0.320 IS units 927.3 134.7 unbinned pixels; need stage -0.075 0.381 if reset Tilting to -34 11.3 -2.7 unbinned pixels; 2.2 3.4 nm along two shift axes; 0.6 4.1 nm on specimen axes Rate = 20.38645 A/sec 7.0 -0.4 unbinned pixels; 1.0 2.3 nm along two shift axes; -0.1 2.5 nm on specimen axes Rate = 12.25242 A/sec Drift is low enough after shot 2 Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.12 nm/sec Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.08 nm/sec Defocus = -0.367 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.14.18_batch_tomo_04_7_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 120.3 In SetExposureForMean 120, change by a factor of 0.9997 would require too small a change in exposure time Image shift 0.079 -0.341 IS units 1001.3 109.5 unbinned pixels; need stage -0.068 0.423 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -6.0 0.5 unbinned pixels; 0.9 1.9 nm along two shift axes; 0.0 -2.1 nm on specimen axes Rate = 10.57734 A/sec Drift is low enough after shot 1 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.06 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -1.940 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.15.21_batch_tomo_04_7_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 123.6 In SetExposureForMean 120, change by a factor of 0.9708 would require too small a change in exposure time Image shift 0.023 -0.209 IS units 588.7 136.2 unbinned pixels; need stage -0.065 0.210 if reset Tilting to 16 Correcting tilt backlash -6.5 -5.8 unbinned pixels; 1.2 2.9 nm along two shift axes; 2.2 -2.1 nm on specimen axes Rate = 15.45654 A/sec -1.5 3.0 unbinned pixels; 1.2 0.1 nm along two shift axes; -1.0 -0.6 nm on specimen axes Rate = 5.93194 A/sec Drift is low enough after shot 2 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.07 nm/sec Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -1.980 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.16.25_batch_tomo_04_7_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 122.0 In SetExposureForMean 120, change by a factor of 0.9843 would require too small a change in exposure time Image shift 0.079 -0.268 IS units 802.6 40.5 unbinned pixels; need stage -0.039 0.294 if reset Tilting to -36 -36.00 degrees 1.8 -3.6 unbinned pixels; 1.4 0.1 nm along two shift axes; 1.2 0.7 nm on specimen axes Rate = 7.07825 A/sec Drift is low enough after shot 1 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.08 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.286 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.17.26_batch_tomo_04_7_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 118.5 In SetExposureForMean 120, change by a factor of 1.0161 would require too small a change in exposure time Image shift 0.100 -0.386 IS units 1144.6 96.1 unbinned pixels; need stage -0.068 0.496 if reset Tilting to -38 9.5 -1.9 unbinned pixels; 1.8 2.9 nm along two shift axes; 0.4 3.4 nm on specimen axes Rate = 17.04239 A/sec 6.5 -1.3 unbinned pixels; 1.2 2.0 nm along two shift axes; 0.3 2.3 nm on specimen axes Rate = 11.68723 A/sec Drift is low enough after shot 2 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.17 nm/sec Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.05 nm/sec Defocus = -0.280 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.18.33_batch_tomo_04_7_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 116.4 In SetExposureForMean 120, change by a factor of 1.0342 would require too small a change in exposure time Image shift 0.114 -0.407 IS units 1214.5 75.7 unbinned pixels; need stage -0.063 0.542 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -6.0 2.2 unbinned pixels; 1.4 1.7 nm along two shift axes; -0.6 -2.2 nm on specimen axes Rate = 11.24644 A/sec Drift is low enough after shot 1 Measured defocus = -3.64 microns changed by -0.11 to target drift = 0.14 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.15 nm/sec Defocus = -2.085 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.19.36_batch_tomo_04_7_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 120.4 In SetExposureForMean 120, change by a factor of 0.9975 would require too small a change in exposure time Image shift 0.040 -0.248 IS units 710.2 128.1 unbinned pixels; need stage -0.066 0.260 if reset Tilting to 20 Correcting tilt backlash -4.7 -1.2 unbinned pixels; 0.2 1.7 nm along two shift axes; 0.6 -1.6 nm on specimen axes Rate = 8.61501 A/sec Drift is low enough after shot 1 Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.08 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -2.093 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.20.35_batch_tomo_04_7_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 118.6 In SetExposureForMean 120, change by a factor of 1.0128 would require too small a change in exposure time Image shift 0.077 -0.340 IS units 997.8 114.2 unbinned pixels; need stage -0.070 0.371 if reset Tilting to -40 -40.00 degrees 4.3 -6.0 unbinned pixels; 2.5 0.7 nm along two shift axes; 2.0 1.7 nm on specimen axes Rate = 13.03911 A/sec Drift is low enough after shot 1 Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.07 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.04 nm/sec Defocus = -0.251 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.21.37_batch_tomo_04_7_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 114.2 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.149 -0.530 IS units 1581.8 99.4 unbinned pixels; need stage -0.083 0.726 if reset Tilting to -42 6.0 -1.8 unbinned pixels; 1.3 1.8 nm along two shift axes; 0.5 2.2 nm on specimen axes Rate = 11.04233 A/sec Drift is low enough after shot 1 Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.14 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.11 nm/sec Defocus = -0.210 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.22.33_batch_tomo_04_7_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 120.1 In SetExposureForMean 120, change by a factor of 1.0036 would require too small a change in exposure time Image shift 0.167 -0.547 IS units 1645.7 64.9 unbinned pixels; need stage -0.073 0.780 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -13.9 2.2 unbinned pixels; 2.3 4.3 nm along two shift axes; -0.3 -4.9 nm on specimen axes Rate = 24.66896 A/sec -6.1 1.4 unbinned pixels; 1.2 1.9 nm along two shift axes; -0.3 -2.2 nm on specimen axes Rate = 11.02045 A/sec Drift is low enough after shot 2 Measured defocus = -3.64 microns changed by -0.11 to target drift = 0.19 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.16 nm/sec Defocus = -2.197 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.23.41_batch_tomo_04_7_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 125.1 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.055 -0.269 IS units 783.4 105.7 unbinned pixels; need stage -0.060 0.295 if reset Tilting to 24 Correcting tilt backlash -3.2 1.5 unbinned pixels; 0.9 0.9 nm along two shift axes; -0.4 -1.2 nm on specimen axes Rate = 6.17875 A/sec Drift is low enough after shot 1 Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.06 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.06 nm/sec Defocus = -2.228 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.24.40_batch_tomo_04_7_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 114.0 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.064 -0.327 IS units 947.4 137.4 unbinned pixels; need stage -0.076 0.362 if reset Tilting to -44 -44.00 degrees 6.1 -7.0 unbinned pixels; 3.0 1.2 nm along two shift axes; 2.3 2.3 nm on specimen axes Rate = 16.21023 A/sec 1.6 2.8 unbinned pixels; 0.7 0.9 nm along two shift axes; -1.0 0.5 nm on specimen axes Rate = 5.63238 A/sec Drift is low enough after shot 2 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.07 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.06 nm/sec Defocus = -0.159 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.25.48_batch_tomo_04_7_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0274 would require too small a change in exposure time Image shift 0.212 -0.654 IS units 1981.0 44.1 unbinned pixels; need stage -0.076 0.972 if reset Tilting to -46 7.0 -6.0 unbinned pixels; 2.8 1.6 nm along two shift axes; 1.9 2.6 nm on specimen axes Rate = 16.11772 A/sec 6.7 1.9 unbinned pixels; 0.2 2.4 nm along two shift axes; -0.9 2.3 nm on specimen axes Rate = 12.23974 A/sec Drift is low enough after shot 2 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.10 nm/sec Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.08 nm/sec Defocus = -0.125 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.26.50_batch_tomo_04_7_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.239 -0.689 IS units 2102.7 4.8 unbinned pixels; need stage -0.066 1.070 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -16.1 1.8 unbinned pixels; 2.5 5.1 nm along two shift axes; -0.1 -5.7 nm on specimen axes Rate = 28.3565 A/sec -6.4 2.3 unbinned pixels; 1.5 1.9 nm along two shift axes; -0.6 -2.3 nm on specimen axes Rate = 11.9687 A/sec Drift is low enough after shot 2 Measured defocus = -3.64 microns changed by -0.11 to target drift = 0.18 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.10 nm/sec Defocus = -2.355 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.28.00_batch_tomo_04_7_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 128.9 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.041 -0.247 IS units 708.6 122.7 unbinned pixels; need stage -0.064 0.274 if reset Tilting to 28 Correcting tilt backlash -5.4 -3.5 unbinned pixels; 0.5 2.2 nm along two shift axes; 1.4 -1.8 nm on specimen axes Rate = 11.32354 A/sec Drift is low enough after shot 1 Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.08 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.02 nm/sec Defocus = -2.350 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.29.00_batch_tomo_04_7_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 117.2 In SetExposureForMean 120, change by a factor of 1.0247 would require too small a change in exposure time Image shift 0.097 -0.388 IS units 1147.3 104.1 unbinned pixels; need stage -0.071 0.456 if reset Tilting to -48 -48.00 degrees 5.8 -6.2 unbinned pixels; 2.7 1.2 nm along two shift axes; 2.0 2.2 nm on specimen axes Rate = 14.81246 A/sec 2.5 3.9 unbinned pixels; 1.0 1.3 nm along two shift axes; -1.4 0.8 nm on specimen axes Rate = 8.13561 A/sec Drift is low enough after shot 2 Measured defocus = -3.85 microns changed by 0.10 to target drift = 0.10 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.012 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.30.07_batch_tomo_04_7_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 111.6 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.287 -0.801 IS units 2452.8 -18.1 unbinned pixels; need stage -0.069 1.297 if reset Tilting to -50 5.9 -6.4 unbinned pixels; 2.8 1.2 nm along two shift axes; 2.1 2.3 nm on specimen axes Rate = 15.35983 A/sec 4.7 2.0 unbinned pixels; 0.1 1.8 nm along two shift axes; -0.8 1.6 nm on specimen axes Rate = 9.00919 A/sec Drift is low enough after shot 2 Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.10 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -0.019 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.31.10_batch_tomo_04_7_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift 0.323 -0.856 IS units 2638.1 -65.8 unbinned pixels; need stage -0.059 1.454 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -14.3 4.2 unbinned pixels; 3.1 4.2 nm along two shift axes; -1.0 -5.2 nm on specimen axes Rate = 26.16967 A/sec -5.5 2.2 unbinned pixels; 1.4 1.5 nm along two shift axes; -0.6 -2.0 nm on specimen axes Rate = 10.35654 A/sec Drift is low enough after shot 2 Measured defocus = -3.65 microns changed by -0.10 to target drift = 0.17 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.16 nm/sec Defocus = -2.431 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.32.20_batch_tomo_04_7_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 130.3 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.067 -0.330 IS units 960.8 130.7 unbinned pixels; need stage -0.075 0.387 if reset Tilting to 32 Correcting tilt backlash -6.8 -8.3 unbinned pixels; 1.9 3.2 nm along two shift axes; 3.1 -2.1 nm on specimen axes Rate = 18.91982 A/sec -2.9 3.5 unbinned pixels; 1.5 0.5 nm along two shift axes; -1.2 -1.1 nm on specimen axes Rate = 7.97738 A/sec Drift is low enough after shot 2 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.08 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -2.464 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.33.25_batch_tomo_04_7_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 118.9 In SetExposureForMean 120, change by a factor of 1.0096 would require too small a change in exposure time Image shift 0.136 -0.459 IS units 1378.5 67.4 unbinned pixels; need stage -0.066 0.572 if reset Tilting to -52 -52.00 degrees 11.7 -8.0 unbinned pixels; 4.0 2.9 nm along two shift axes; 2.4 4.4 nm on specimen axes Rate = 24.88952 A/sec 4.6 2.2 unbinned pixels; 0.2 1.8 nm along two shift axes; -0.9 1.6 nm on specimen axes Rate = 8.96755 A/sec Drift is low enough after shot 2 Measured defocus = -3.85 microns changed by 0.10 to target drift = 0.11 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.12 nm/sec Defocus = 0.058 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.34.32_batch_tomo_04_7_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 111.8 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift 0.378 -0.971 IS units 3001.8 -104.2 unbinned pixels; need stage -0.057 1.729 if reset Tilting to -54 6.6 -4.5 unbinned pixels; 2.3 1.6 nm along two shift axes; 1.4 2.4 nm on specimen axes Rate = 13.97321 A/sec Drift is low enough after shot 1 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.18 nm/sec Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.08 nm/sec Defocus = 0.054 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.35.30_batch_tomo_04_7_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.398 -1.021 IS units 3157.0 -111.6 unbinned pixels; need stage -0.059 1.905 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -17.7 4.5 unbinned pixels; 3.5 5.3 nm along two shift axes; -1.0 -6.3 nm on specimen axes Rate = 31.9227 A/sec -6.0 3.4 unbinned pixels; 1.8 1.6 nm along two shift axes; -1.0 -2.2 nm on specimen axes Rate = 12.06787 A/sec Drift is low enough after shot 2 Measured defocus = -3.70 microns changed by -0.05 to target drift = 0.24 nm/sec Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.16 nm/sec Defocus = -2.550 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.36.40_batch_tomo_04_7_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 130.5 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift 0.107 -0.417 IS units 1236.1 106.6 unbinned pixels; need stage -0.075 0.523 if reset Tilting to 36 Correcting tilt backlash -8.1 -5.2 unbinned pixels; 0.8 3.3 nm along two shift axes; 2.1 -2.7 nm on specimen axes Rate = 17.05833 A/sec -2.8 1.7 unbinned pixels; 0.9 0.7 nm along two shift axes; -0.5 -1.0 nm on specimen axes Rate = 5.77892 A/sec Drift is low enough after shot 2 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.12 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -2.582 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.37.45_batch_tomo_04_7_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 118.2 In SetExposureForMean 120, change by a factor of 1.0155 would require too small a change in exposure time Image shift 0.152 -0.536 IS units 1602.8 97.7 unbinned pixels; need stage -0.083 0.697 if reset Tilting to -56 -56.00 degrees 10.2 -10.4 unbinned pixels; 4.6 2.1 nm along two shift axes; 3.3 3.9 nm on specimen axes Rate = 25.527 A/sec 4.9 2.3 unbinned pixels; 0.2 1.9 nm along two shift axes; -0.9 1.7 nm on specimen axes Rate = 9.55227 A/sec Drift is low enough after shot 2 Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.06 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.07 nm/sec Defocus = 0.059 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.38.53_batch_tomo_04_7_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 109.8 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.469 -1.170 IS units 3631.5 -164.1 unbinned pixels; need stage -0.055 2.306 if reset Tilting to -58 4.3 -0.1 unbinned pixels; 0.5 1.4 nm along two shift axes; -0.1 1.5 nm on specimen axes Rate = 7.49124 A/sec Drift is low enough after shot 1 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.08 nm/sec Measured defocus = -3.84 microns changed by 0.09 to target drift = 0.06 nm/sec Defocus = 0.128 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.39.51_batch_tomo_04_7_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 117.6 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.477 -1.188 IS units 3688.9 -167.1 unbinned pixels; need stage -0.056 2.472 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -13.6 1.7 unbinned pixels; 2.2 4.3 nm along two shift axes; -0.2 -4.8 nm on specimen axes Rate = 24.02755 A/sec -9.3 2.3 unbinned pixels; 1.8 2.8 nm along two shift axes; -0.5 -3.3 nm on specimen axes Rate = 16.70642 A/sec -6.5 1.8 unbinned pixels; 1.4 1.9 nm along two shift axes; -0.4 -2.3 nm on specimen axes Rate = 11.84722 A/sec Drift is low enough after shot 3 Measured defocus = -3.67 microns changed by -0.08 to target drift = 0.12 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.06 nm/sec Defocus = -2.680 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.41.07_batch_tomo_04_7_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 135.2 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.167 -0.538 IS units 1622.2 56.5 unbinned pixels; need stage -0.069 0.726 if reset Tilting to 40 Correcting tilt backlash -6.7 -5.5 unbinned pixels; 1.1 2.9 nm along two shift axes; 2.1 -2.2 nm on specimen axes Rate = 15.33492 A/sec -2.5 1.1 unbinned pixels; 0.7 0.7 nm along two shift axes; -0.3 -0.9 nm on specimen axes Rate = 4.77609 A/sec Drift is low enough after shot 2 Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.09 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -2.678 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.42.12_batch_tomo_04_7_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 115.5 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.208 -0.640 IS units 1938.2 43.4 unbinned pixels; need stage -0.075 0.893 if reset Tilting to -60 -60.00 degrees 7.9 -7.4 unbinned pixels; 3.4 1.7 nm along two shift axes; 2.3 3.0 nm on specimen axes Rate = 18.98206 A/sec 2.8 2.2 unbinned pixels; 0.4 1.2 nm along two shift axes; -0.9 0.9 nm on specimen axes Rate = 6.32246 A/sec Drift is low enough after shot 2 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.08 nm/sec Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.09 nm/sec Defocus = 0.130 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.43.21_batch_tomo_04_7_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 111.7 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.526 -1.277 IS units 3976.8 -216.9 unbinned pixels; need stage -0.048 2.826 if reset Tilting to -62 Trimming A by 25 30, reference by 20 20 Trimming A by 30 30, reference by 20 20 1.8 -0.0 unbinned pixels; 0.2 0.6 nm along two shift axes; -0.1 0.6 nm on specimen axes Rate = 3.18792 A/sec Drift is low enough after shot 1 Measured defocus = -3.66 microns changed by -0.09 to target drift = 0.13 nm/sec Measured defocus = -3.91 microns changed by 0.16 to target drift = 0.11 nm/sec Defocus = 0.202 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.44.19_batch_tomo_04_7_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 116.7 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift 0.521 -1.273 IS units 3963.1 -207.3 unbinned pixels; need stage -0.051 2.999 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -16.4 2.1 unbinned pixels; 2.6 5.2 nm along two shift axes; -0.2 -5.8 nm on specimen axes Rate = 28.98096 A/sec -9.8 1.3 unbinned pixels; 1.6 3.1 nm along two shift axes; -0.2 -3.5 nm on specimen axes Rate = 17.34098 A/sec -7.5 0.2 unbinned pixels; 1.0 2.5 nm along two shift axes; 0.2 -2.6 nm on specimen axes Rate = 13.17746 A/sec Drift is low enough after shot 3 Measured defocus = -3.67 microns changed by -0.08 to target drift = 0.11 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -2.746 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.45.36_batch_tomo_04_7_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 135.8 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.208 -0.616 IS units 1874.7 20.3 unbinned pixels; need stage -0.065 0.891 if reset Tilting to 44 Correcting tilt backlash -8.9 -7.9 unbinned pixels; 1.6 3.9 nm along two shift axes; 3.0 -2.9 nm on specimen axes Rate = 20.9995 A/sec 1.0 1.8 unbinned pixels; 0.5 0.5 nm along two shift axes; -0.6 0.3 nm on specimen axes Rate = 3.56863 A/sec Drift is low enough after shot 2 Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.07 nm/sec Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -2.754 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.46.42_batch_tomo_04_7_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 116.0 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.248 -0.706 IS units 2158.3 -3.2 unbinned pixels; need stage -0.065 1.061 if reset Tilting to -64 -64.00 degrees Trimming A by 20 85, reference by 40 40 Trimming A by 20 75, reference by 40 40 0.7 -7.3 unbinned pixels; 2.5 0.6 nm along two shift axes; 2.5 0.5 nm on specimen axes Rate = 12.86931 A/sec Drift is low enough after shot 1 Measured defocus = -3.87 microns changed by 0.12 to target drift = 0.04 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.01 nm/sec Defocus = 0.305 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.47.47_batch_tomo_04_7_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 109.3 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.564 -1.347 IS units 4203.8 -252.5 unbinned pixels; need stage -0.042 3.409 if reset Tilting to -66 Trimming A by 20 80, reference by 20 75 Trimming A by 20 75, reference by 20 75 2.4 0.2 unbinned pixels; 0.2 0.8 nm along two shift axes; -0.1 0.9 nm on specimen axes Rate = 4.29648 A/sec Drift is low enough after shot 1 Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.07 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.01 nm/sec Defocus = 0.357 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.48.45_batch_tomo_04_7_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 111.3 In SetExposureForMean 120, exposure time changed to 2.8800 Image shift 0.572 -1.344 IS units 4205.2 -278.1 unbinned pixels; need stage -0.034 3.678 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -15.7 2.6 unbinned pixels; 2.7 4.9 nm along two shift axes; -0.4 -5.6 nm on specimen axes Rate = 27.95185 A/sec -9.9 2.6 unbinned pixels; 2.0 3.0 nm along two shift axes; -0.6 -3.6 nm on specimen axes Rate = 17.98669 A/sec -6.3 2.1 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.3 nm on specimen axes Rate = 11.66199 A/sec Drift is low enough after shot 3 Measured defocus = -3.69 microns changed by -0.06 to target drift = 0.08 nm/sec Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.13 nm/sec Defocus = -2.814 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.50.03_batch_tomo_04_7_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 138.1 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift 0.259 -0.697 IS units 2143.9 -43.0 unbinned pixels; need stage -0.051 1.093 if reset Tilting to 48 Correcting tilt backlash -4.9 -0.9 unbinned pixels; 0.3 1.7 nm along two shift axes; 0.5 -1.7 nm on specimen axes Rate = 8.7335 A/sec Drift is low enough after shot 1 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.09 nm/sec Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.02 nm/sec Defocus = -2.893 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.51.08_batch_tomo_04_7_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 111.8 In SetExposureForMean 120, exposure time changed to 2.7600 Image shift 0.283 -0.750 IS units 2309.4 -56.3 unbinned pixels; need stage -0.052 1.223 if reset Tilting to -68 -68.00 degrees Trimming A by 40 40, reference by 20 80 Trimming A by 20 80, reference by 20 80 11.3 -7.8 unbinned pixels; 3.9 2.8 nm along two shift axes; 2.4 4.2 nm on specimen axes Rate = 24.08987 A/sec Trimming A by 20 80, reference by 20 80 2.3 2.4 unbinned pixels; 0.5 1.1 nm along two shift axes; -0.9 0.7 nm on specimen axes Rate = 5.88978 A/sec Drift is low enough after shot 2 Measured defocus = -3.88 microns changed by 0.13 to target drift = 0.02 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = 0.492 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_20.52.19_batch_tomo_04_7_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 107.0 In SetExposureForMean 120, exposure time changed to 3.2400 Image shift 0.618 -1.419 IS units 4453.0 -332.8 unbinned pixels; need stage -0.022 4.232 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 0.64 microns changed by -4.39 to target drift = 0.52 nm/sec Focus area changed from stored state, axis position 2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_05.st Running script 1 at item 9 (-380.15, -258.62) 20:53:13 Refine ZLP: additional offset = -0.7 eV; total offset = -12.5 eV Item 9: Stage: -380.15 -258.62 -223 Label: 9 Note: Sec 8 - anchor_maps.map Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Target defocus is: -3.75 um Target defocus is: -4.00 um Target defocus = -4 Trimming A by 21 155, reference by 21 155 Angle = -4.40, increment = 0.60, shift = -19.0 -7.1 pixels, -0.045 um Y Trimming A by 21 155, reference by 21 155 Angle = -3.20, increment = 1.80, shift = -87.9 -17.0 pixels, -0.204 um Y Trimming A by 21 155, reference by 21 155 Angle = 0.41, increment = 5.41, shift = -240.9 -39.2 pixels, -0.556 um Y Trimming A by 21 155, reference by 21 155 Angle = 3.00, increment = 8.00, shift = -345.8 -56.7 pixels, -0.799 um Y Rough eucentricity: changing Z by 5.73 to -217.65, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 25 150, reference by 34 150 Angle = 18.00, shift = -73.9, -1.4 pixels, 0.016, -0.168 um, IS = -0.016, 0.168 Trimming A by 25 150, reference by 25 150 Angle = 11.00, shift = -77.1, -3.6 pixels, 0.011, -0.176 um, IS = -0.027, 0.344 Trimming A by 20 150, reference by 25 150 Angle = 4.00, shift = -66.9, -3.6 pixels, 0.009, -0.153 um, IS = -0.036, 0.497 Trimming A by 22 34 150 20, reference by 21 51 145 20 Angle = -4.00, shift = -33.0, -18.4 pixels, -0.033, -0.079 um, IS = -0.003, 0.576 Trimming A by 33 150, reference by 33 150 Angle = -11.00, shift = -4.6, -13.1 pixels, -0.029, -0.014 um, IS = 0.026, 0.590 Trimming A by 22 150, reference by 33 150 Angle = -18.00, shift = -18.5, 4.1 pixels, 0.014, -0.041 um, IS = 0.012, 0.631 Trimming A by 21 150, reference by 21 150 Angle = -24.00, shift = -58.5, -3.3 pixels, 0.007, -0.134 um, IS = 0.005, 0.764 General solution delZ 0.84, delY 1.24, yZero 0.51 solution with assumed YZero 0.54: delZ 0.84, delY 1.55 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.84 microns and lateral displacement of -1.24 microns Trimming A by 21 42 140 20, reference by 21 56 140 20 Disparity in stage position after aligning to center of map frame: 0.01 -1.33 Trimming A by 21 56 140 20, reference by 21 56 140 20 Disparity in stage position after aligning to target position: 0.42 -1.73 (2nd move error 0.04 0.13) Disparity in stage position after aligning to map item itself: 0.02 -1.20 (align shift -0.04 -0.04) Finished aligning to item in 3 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -380.13 -259.92 -218.47 0.3 -1.3 unbinned pixels; 0.5 0.1 nm along two shift axes; 0.5 0.1 nm on specimen axes Rate = 2.41696 A/sec Drift is low enough after shot 1 Measured defocus = 1.34 microns changed by -5.34 to target drift = 0.04 nm/sec Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.04 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -1.441 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.02.19_batch_tomo_05_9_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 107.2 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift 0.041 -0.082 IS units 263.2 -34.5 unbinned pixels; need stage 0.004 0.096 if reset Tilting to -8 Correcting tilt backlash 8.9 -1.2 unbinned pixels; 1.4 2.8 nm along two shift axes; 0.1 3.1 nm on specimen axes Rate = 15.70232 A/sec 8.2 0.3 unbinned pixels; 0.8 2.8 nm along two shift axes; -0.4 2.9 nm on specimen axes Rate = 14.42388 A/sec 3.7 0.3 unbinned pixels; 0.3 1.3 nm along two shift axes; -0.2 1.3 nm on specimen axes Rate = 6.50089 A/sec Drift is low enough after shot 3 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.07 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.519 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.03.29_batch_tomo_05_9_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 117.6 In SetExposureForMean 120, change by a factor of 1.0204 would require too small a change in exposure time Image shift 0.001 -0.074 IS units 200.4 68.5 unbinned pixels; need stage -0.030 0.069 if reset Tilting to -12 -12.00 degrees -10.5 1.1 unbinned pixels; 1.6 3.4 nm along two shift axes; -0.1 -3.7 nm on specimen axes Rate = 18.56861 A/sec -5.7 5.5 unbinned pixels; 2.5 1.2 nm along two shift axes; -1.8 -2.2 nm on specimen axes Rate = 13.92141 A/sec Drift is low enough after shot 2 Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.12 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -1.341 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.04.32_batch_tomo_05_9_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 117.6 In SetExposureForMean 120, change by a factor of 1.0211 would require too small a change in exposure time Image shift -0.050 0.008 IS units -70.3 127.9 unbinned pixels; need stage -0.042 -0.030 if reset Tilting to -14 8.1 -2.1 unbinned pixels; 1.6 2.4 nm along two shift axes; 0.5 2.9 nm on specimen axes Rate = 14.59858 A/sec 10.7 0.9 unbinned pixels; 0.9 3.7 nm along two shift axes; -0.7 3.7 nm on specimen axes Rate = 18.84138 A/sec 7.8 -1.0 unbinned pixels; 1.2 2.5 nm along two shift axes; 0.1 2.8 nm on specimen axes Rate = 13.82348 A/sec Drift is low enough after shot 3 Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.09 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -1.234 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.05.44_batch_tomo_05_9_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 117.7 In SetExposureForMean 120, change by a factor of 1.0206 would require too small a change in exposure time Image shift -0.086 0.091 IS units -329.6 147.0 unbinned pixels; need stage -0.040 -0.125 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 12.6 -3.8 unbinned pixels; 2.7 3.7 nm along two shift axes; 0.9 4.5 nm on specimen axes Rate = 23.10336 A/sec 5.8 -1.2 unbinned pixels; 1.1 1.8 nm along two shift axes; 0.2 2.1 nm on specimen axes Rate = 10.4095 A/sec Drift is low enough after shot 2 Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.12 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -1.548 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.06.48_batch_tomo_05_9_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0211 would require too small a change in exposure time Image shift 0.026 -0.119 IS units 347.0 42.4 unbinned pixels; need stage -0.025 0.122 if reset Tilting to -4 Correcting tilt backlash -7.2 1.1 unbinned pixels; 1.2 2.2 nm along two shift axes; -0.2 -2.5 nm on specimen axes Rate = 12.69017 A/sec Drift is low enough after shot 1 Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.09 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -1.595 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.07.47_batch_tomo_05_9_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 117.0 In SetExposureForMean 120, change by a factor of 1.0260 would require too small a change in exposure time Image shift 0.009 -0.083 IS units 235.2 54.0 unbinned pixels; need stage -0.026 0.082 if reset Tilting to -16 -16.00 degrees -4.3 0.1 unbinned pixels; 0.5 1.4 nm along two shift axes; 0.1 -1.5 nm on specimen axes Rate = 7.54116 A/sec Drift is low enough after shot 1 Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.04 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -1.122 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.08.45_batch_tomo_05_9_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0234 would require too small a change in exposure time Image shift -0.091 0.085 IS units -319.0 166.6 unbinned pixels; need stage -0.048 -0.123 if reset Tilting to -18 13.5 -8.6 unbinned pixels; 4.4 3.5 nm along two shift axes; 2.6 5.0 nm on specimen axes Rate = 28.11405 A/sec 9.9 0.5 unbinned pixels; 1.0 3.3 nm along two shift axes; -0.5 3.5 nm on specimen axes Rate = 17.36919 A/sec 5.8 -0.9 unbinned pixels; 1.0 1.8 nm along two shift axes; 0.1 2.1 nm on specimen axes Rate = 10.37028 A/sec Drift is low enough after shot 3 Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.14 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -1.059 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.09.54_batch_tomo_05_9_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 117.3 In SetExposureForMean 120, change by a factor of 1.0243 would require too small a change in exposure time Image shift -0.050 0.023 IS units -112.2 113.4 unbinned pixels; need stage -0.036 -0.046 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 8.5 -1.4 unbinned pixels; 1.4 2.6 nm along two shift axes; 0.2 3.0 nm on specimen axes Rate = 15.08604 A/sec 8.0 -0.3 unbinned pixels; 1.0 2.6 nm along two shift axes; -0.1 2.8 nm on specimen axes Rate = 13.97877 A/sec Drift is low enough after shot 2 Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.06 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -1.613 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.10.59_batch_tomo_05_9_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 116.7 In SetExposureForMean 120, change by a factor of 1.0283 would require too small a change in exposure time Image shift 0.013 -0.081 IS units 231.7 42.7 unbinned pixels; need stage -0.022 0.081 if reset Tilting to 0 Correcting tilt backlash -13.4 1.4 unbinned pixels; 2.0 4.3 nm along two shift axes; -0.1 -4.8 nm on specimen axes Rate = 23.67063 A/sec -5.9 2.5 unbinned pixels; 1.5 1.7 nm along two shift axes; -0.7 -2.2 nm on specimen axes Rate = 11.28589 A/sec Drift is low enough after shot 2 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.20 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.17 nm/sec Defocus = -1.674 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.12.03_batch_tomo_05_9_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 115.3 In SetExposureForMean 120, change by a factor of 1.0411 would require too small a change in exposure time Image shift -0.004 -0.051 IS units 133.4 59.7 unbinned pixels; need stage -0.025 0.045 if reset Tilting to -20 -20.00 degrees -3.6 0.2 unbinned pixels; 0.5 1.2 nm along two shift axes; 0.0 -1.3 nm on specimen axes Rate = 6.41204 A/sec Drift is low enough after shot 1 Measured defocus = -4.13 microns changed by 0.13 to target drift = 0.01 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.01 nm/sec Defocus = -0.921 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.13.03_batch_tomo_05_9_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 116.6 In SetExposureForMean 120, change by a factor of 1.0291 would require too small a change in exposure time Image shift -0.084 0.070 IS units -271.7 162.9 unbinned pixels; need stage -0.048 -0.108 if reset Tilting to -22 13.3 -3.2 unbinned pixels; 2.6 4.0 nm along two shift axes; 0.7 4.8 nm on specimen axes Rate = 23.98111 A/sec 9.4 -1.5 unbinned pixels; 1.6 2.9 nm along two shift axes; 0.3 3.3 nm on specimen axes Rate = 16.69457 A/sec 6.7 -1.3 unbinned pixels; 1.2 2.1 nm along two shift axes; 0.2 2.4 nm on specimen axes Rate = 12.02398 A/sec Drift is low enough after shot 3 Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.16 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.16 nm/sec Defocus = -0.877 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.14.10_batch_tomo_05_9_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 116.0 In SetExposureForMean 120, change by a factor of 1.0342 would require too small a change in exposure time Image shift -0.067 0.062 IS units -234.0 124.1 unbinned pixels; need stage -0.036 -0.094 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 6.8 0.6 unbinned pixels; 0.6 2.3 nm along two shift axes; -0.4 2.4 nm on specimen axes Rate = 11.94999 A/sec Drift is low enough after shot 1 Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.10 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -1.721 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.15.11_batch_tomo_05_9_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 114.5 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.010 -0.051 IS units 128.7 76.9 unbinned pixels; need stage -0.030 0.043 if reset Tilting to 4 Correcting tilt backlash -5.2 -3.2 unbinned pixels; 0.5 2.1 nm along two shift axes; 1.3 -1.7 nm on specimen axes Rate = 10.80299 A/sec Drift is low enough after shot 1 Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.17 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.18 nm/sec Defocus = -1.784 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.16.09_batch_tomo_05_9_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 124.3 In SetExposureForMean 120, change by a factor of 0.9657 would require too small a change in exposure time Image shift 0.017 -0.093 IS units 269.3 43.3 unbinned pixels; need stage -0.023 0.094 if reset Tilting to -24 -24.00 degrees -1.8 0.7 unbinned pixels; 0.4 0.5 nm along two shift axes; -0.2 -0.7 nm on specimen axes Rate = 3.39373 A/sec Drift is low enough after shot 1 Measured defocus = -4.11 microns changed by 0.11 to target drift = 0.01 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.756 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.17.09_batch_tomo_05_9_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 126.2 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift -0.083 0.060 IS units -243.0 168.4 unbinned pixels; need stage -0.050 -0.100 if reset Tilting to -26 16.6 -7.1 unbinned pixels; 4.3 4.6 nm along two shift axes; 2.0 6.0 nm on specimen axes Rate = 31.5589 A/sec 8.1 0.0 unbinned pixels; 0.9 2.7 nm along two shift axes; -0.3 2.8 nm on specimen axes Rate = 14.19372 A/sec 3.5 -0.7 unbinned pixels; 0.6 1.1 nm along two shift axes; 0.1 1.2 nm on specimen axes Rate = 6.25009 A/sec Drift is low enough after shot 3 Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.23 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.17 nm/sec Defocus = -0.709 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.18.18_batch_tomo_05_9_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 115.0 In SetExposureForMean 120, change by a factor of 1.0452 would require too small a change in exposure time Image shift -0.058 0.040 IS units -167.2 120.4 unbinned pixels; need stage -0.036 -0.070 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 5.6 -0.1 unbinned pixels; 0.7 1.8 nm along two shift axes; -0.2 2.0 nm on specimen axes Rate = 9.76807 A/sec Drift is low enough after shot 1 Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.04 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -1.816 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.19.20_batch_tomo_05_9_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 112.8 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.005 -0.075 IS units 198.0 84.9 unbinned pixels; need stage -0.035 0.068 if reset Tilting to 8 Correcting tilt backlash -5.7 1.4 unbinned pixels; 1.1 1.7 nm along two shift axes; -0.3 -2.0 nm on specimen axes Rate = 10.27491 A/sec Drift is low enough after shot 1 Measured defocus = -3.90 microns changed by -0.10 to target drift = 0.17 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.13 nm/sec Defocus = -1.922 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.20.18_batch_tomo_05_9_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 122.0 In SetExposureForMean 120, change by a factor of 0.9840 would require too small a change in exposure time Image shift 0.011 -0.102 IS units 288.3 66.2 unbinned pixels; need stage -0.032 0.101 if reset Tilting to -28 -28.00 degrees -4.8 0.4 unbinned pixels; 0.7 1.5 nm along two shift axes; 0.0 -1.7 nm on specimen axes Rate = 8.44915 A/sec Drift is low enough after shot 1 Measured defocus = -4.14 microns changed by 0.14 to target drift = 0.07 nm/sec Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.02 nm/sec Defocus = -0.533 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.21.19_batch_tomo_05_9_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 124.9 In SetExposureForMean 120, change by a factor of 0.9634 would require too small a change in exposure time Image shift -0.072 0.015 IS units -111.8 181.3 unbinned pixels; need stage -0.059 -0.051 if reset Tilting to -30 8.2 -6.4 unbinned pixels; 3.1 2.0 nm along two shift axes; 2.0 3.1 nm on specimen axes Rate = 18.25166 A/sec 7.5 1.0 unbinned pixels; 0.6 2.6 nm along two shift axes; -0.6 2.6 nm on specimen axes Rate = 13.35535 A/sec Drift is low enough after shot 2 Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.27 nm/sec Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.13 nm/sec Defocus = -0.499 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.22.21_batch_tomo_05_9_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 123.7 In SetExposureForMean 120, change by a factor of 0.9726 would require too small a change in exposure time Image shift -0.038 -0.023 IS units 25.9 126.4 unbinned pixels; need stage -0.044 0.006 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -5.0 1.6 unbinned pixels; 1.1 1.5 nm along two shift axes; -0.4 -1.8 nm on specimen axes Rate = 9.17574 A/sec Drift is low enough after shot 1 Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.13 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.12 nm/sec Defocus = -1.940 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.23.23_batch_tomo_05_9_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 120.6 In SetExposureForMean 120, change by a factor of 0.9957 would require too small a change in exposure time Image shift -0.018 -0.076 IS units 186.7 122.1 unbinned pixels; need stage -0.048 0.063 if reset Tilting to 12 Correcting tilt backlash -10.0 -1.9 unbinned pixels; 0.5 3.5 nm along two shift axes; 1.0 -3.4 nm on specimen axes Rate = 17.87341 A/sec -5.7 2.9 unbinned pixels; 1.6 1.6 nm along two shift axes; -0.8 -2.1 nm on specimen axes Rate = 11.22582 A/sec Drift is low enough after shot 2 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.18 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -1.998 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.24.27_batch_tomo_05_9_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 119.2 In SetExposureForMean 120, change by a factor of 1.0073 would require too small a change in exposure time Image shift 0.014 -0.138 IS units 387.0 92.6 unbinned pixels; need stage -0.044 0.137 if reset Tilting to -32 -32.00 degrees -0.3 -2.8 unbinned pixels; 0.9 0.4 nm along two shift axes; 1.0 -0.0 nm on specimen axes Rate = 4.97617 A/sec Drift is low enough after shot 1 Measured defocus = -4.15 microns changed by 0.15 to target drift = 0.08 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.13 nm/sec Defocus = -0.349 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.25.28_batch_tomo_05_9_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 122.8 In SetExposureForMean 120, change by a factor of 0.9808 would require too small a change in exposure time Image shift -0.023 -0.108 IS units 270.9 165.5 unbinned pixels; need stage -0.065 0.107 if reset Tilting to -34 9.5 -7.1 unbinned pixels; 3.5 2.3 nm along two shift axes; 2.2 3.5 nm on specimen axes Rate = 20.72919 A/sec 8.5 0.6 unbinned pixels; 0.8 2.9 nm along two shift axes; -0.5 3.0 nm on specimen axes Rate = 14.94672 A/sec 6.0 -0.5 unbinned pixels; 0.9 1.9 nm along two shift axes; -0.0 2.1 nm on specimen axes Rate = 10.47168 A/sec Drift is low enough after shot 3 Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.11 nm/sec Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.16 nm/sec Defocus = -0.317 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.26.41_batch_tomo_05_9_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 121.3 In SetExposureForMean 120, change by a factor of 0.9932 would require too small a change in exposure time Image shift 0.006 -0.139 IS units 383.0 114.6 unbinned pixels; need stage -0.051 0.159 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -8.7 -0.3 unbinned pixels; 0.9 2.9 nm along two shift axes; 0.4 -3.1 nm on specimen axes Rate = 15.34256 A/sec -2.0 4.0 unbinned pixels; 1.6 0.2 nm along two shift axes; -1.3 -0.8 nm on specimen axes Rate = 7.91775 A/sec Drift is low enough after shot 2 Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.14 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.08 nm/sec Defocus = -2.056 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.27.49_batch_tomo_05_9_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0221 would require too small a change in exposure time Image shift -0.008 -0.095 IS units 249.4 111.9 unbinned pixels; need stage -0.046 0.087 if reset Tilting to 16 Correcting tilt backlash -6.2 -0.4 unbinned pixels; 0.6 2.1 nm along two shift axes; 0.3 -2.2 nm on specimen axes Rate = 10.87168 A/sec Drift is low enough after shot 1 Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.12 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -2.089 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.28.47_batch_tomo_05_9_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, change by a factor of 1.0378 would require too small a change in exposure time Image shift 0.017 -0.147 IS units 415.0 94.1 unbinned pixels; need stage -0.045 0.149 if reset Tilting to -36 -36.00 degrees 3.3 -5.3 unbinned pixels; 2.1 0.4 nm along two shift axes; 1.7 1.3 nm on specimen axes Rate = 10.88659 A/sec Drift is low enough after shot 1 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.09 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.08 nm/sec Defocus = -0.221 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.29.49_batch_tomo_05_9_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 120.2 In SetExposureForMean 120, change by a factor of 1.0031 would require too small a change in exposure time Image shift 0.033 -0.228 IS units 650.5 127.4 unbinned pixels; need stage -0.064 0.279 if reset Tilting to -38 7.3 -7.6 unbinned pixels; 3.4 1.5 nm along two shift axes; 2.4 2.8 nm on specimen axes Rate = 18.48033 A/sec 8.0 0.9 unbinned pixels; 0.6 2.8 nm along two shift axes; -0.6 2.8 nm on specimen axes Rate = 14.12077 A/sec 3.7 0.3 unbinned pixels; 0.3 1.2 nm along two shift axes; -0.2 1.3 nm on specimen axes Rate = 6.43993 A/sec Drift is low enough after shot 3 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.18 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.14 nm/sec Defocus = -0.129 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.30.57_batch_tomo_05_9_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 118.6 In SetExposureForMean 120, change by a factor of 1.0171 would require too small a change in exposure time Image shift 0.072 -0.277 IS units 822.5 68.6 unbinned pixels; need stage -0.049 0.366 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -8.4 1.4 unbinned pixels; 1.4 2.6 nm along two shift axes; -0.2 -3.0 nm on specimen axes Rate = 14.99573 A/sec -7.3 0.6 unbinned pixels; 1.0 2.3 nm along two shift axes; 0.0 -2.6 nm on specimen axes Rate = 12.81832 A/sec Drift is low enough after shot 2 Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.20 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.19 nm/sec Defocus = -2.150 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.32.05_batch_tomo_05_9_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 113.8 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.022 -0.142 IS units 406.0 74.0 unbinned pixels; need stage -0.038 0.148 if reset Tilting to 20 Correcting tilt backlash -7.7 -2.4 unbinned pixels; 0.1 2.8 nm along two shift axes; 1.1 -2.6 nm on specimen axes Rate = 14.19062 A/sec -1.8 4.5 unbinned pixels; 1.7 0.0 nm along two shift axes; -1.5 -0.8 nm on specimen axes Rate = 8.50382 A/sec Drift is low enough after shot 2 Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.10 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -2.143 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.33.09_batch_tomo_05_9_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 120.8 In SetExposureForMean 120, change by a factor of 0.9958 would require too small a change in exposure time Image shift 0.052 -0.220 IS units 647.5 68.7 unbinned pixels; need stage -0.044 0.241 if reset Tilting to -40 -40.00 degrees 9.1 -8.3 unbinned pixels; 3.8 2.0 nm along two shift axes; 2.6 3.4 nm on specimen axes Rate = 21.59788 A/sec 4.1 1.2 unbinned pixels; 0.1 1.5 nm along two shift axes; -0.6 1.4 nm on specimen axes Rate = 7.48573 A/sec Drift is low enough after shot 2 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.12 nm/sec Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.06 nm/sec Defocus = 0.035 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.34.17_batch_tomo_05_9_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 126.8 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.121 -0.400 IS units 1203.2 50.9 unbinned pixels; need stage -0.055 0.553 if reset Tilting to -42 8.1 -7.1 unbinned pixels; 3.3 1.8 nm along two shift axes; 2.2 3.1 nm on specimen axes Rate = 18.96688 A/sec 8.3 0.2 unbinned pixels; 0.9 2.8 nm along two shift axes; -0.3 2.9 nm on specimen axes Rate = 14.55346 A/sec 4.6 -0.0 unbinned pixels; 0.5 1.5 nm along two shift axes; -0.1 1.6 nm on specimen axes Rate = 8.07217 A/sec Drift is low enough after shot 3 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.18 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.20 nm/sec Defocus = 0.086 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.35.25_batch_tomo_05_9_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 115.1 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.146 -0.429 IS units 1306.4 11.7 unbinned pixels; need stage -0.044 0.621 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -19.9 -0.9 unbinned pixels; 2.0 6.7 nm along two shift axes; 0.9 -7.0 nm on specimen axes Rate = 34.99116 A/sec -11.0 1.6 unbinned pixels; 1.8 3.5 nm along two shift axes; -0.2 -3.9 nm on specimen axes Rate = 19.49854 A/sec -5.9 2.3 unbinned pixels; 1.5 1.7 nm along two shift axes; -0.6 -2.1 nm on specimen axes Rate = 11.03404 A/sec Drift is low enough after shot 3 Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.28 nm/sec Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.31 nm/sec Defocus = -2.143 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.36.39_batch_tomo_05_9_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 118.5 In SetExposureForMean 120, change by a factor of 1.0161 would require too small a change in exposure time Image shift 0.052 -0.200 IS units 594.5 48.4 unbinned pixels; need stage -0.035 0.225 if reset Tilting to 24 Correcting tilt backlash -1.8 -2.4 unbinned pixels; 0.6 0.9 nm along two shift axes; 0.9 -0.6 nm on specimen axes Rate = 5.2982 A/sec Drift is low enough after shot 1 Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.13 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.131 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.37.38_batch_tomo_05_9_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.092 -0.333 IS units 993.8 67.0 unbinned pixels; need stage -0.054 0.382 if reset Tilting to -44 -44.00 degrees 12.7 -8.3 unbinned pixels; 4.3 3.2 nm along two shift axes; 2.5 4.7 nm on specimen axes Rate = 26.66933 A/sec 6.4 1.4 unbinned pixels; 0.3 2.3 nm along two shift axes; -0.7 2.2 nm on specimen axes Rate = 11.60042 A/sec Drift is low enough after shot 2 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.17 nm/sec Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.14 nm/sec Defocus = 0.107 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.38.45_batch_tomo_05_9_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 132.1 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.198 -0.566 IS units 1728.2 -0.5 unbinned pixels; need stage -0.053 0.850 if reset Tilting to -46 4.4 -7.7 unbinned pixels; 3.1 0.5 nm along two shift axes; 2.6 1.8 nm on specimen axes Rate = 15.51896 A/sec 8.5 2.1 unbinned pixels; 0.3 3.1 nm along two shift axes; -1.0 2.9 nm on specimen axes Rate = 15.39305 A/sec 5.4 -0.2 unbinned pixels; 0.7 1.8 nm along two shift axes; -0.1 1.9 nm on specimen axes Rate = 9.49827 A/sec Drift is low enough after shot 3 Measured defocus = -4.16 microns changed by 0.16 to target drift = 0.16 nm/sec Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.13 nm/sec Defocus = 0.305 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.39.54_batch_tomo_05_9_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 120.6 In SetExposureForMean 120, change by a factor of 1.0002 would require too small a change in exposure time Image shift 0.241 -0.630 IS units 1944.5 -57.6 unbinned pixels; need stage -0.040 0.992 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -17.0 1.0 unbinned pixels; 2.3 5.5 nm along two shift axes; 0.2 -6.0 nm on specimen axes Rate = 29.83907 A/sec -9.2 2.3 unbinned pixels; 1.8 2.8 nm along two shift axes; -0.5 -3.3 nm on specimen axes Rate = 16.67447 A/sec -8.6 1.2 unbinned pixels; 1.4 2.7 nm along two shift axes; -0.2 -3.1 nm on specimen axes Rate = 15.30488 A/sec -5.8 1.7 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.4 -2.1 nm on specimen axes Rate = 10.67279 A/sec Drift is low enough after shot 4 Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.15 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.15 nm/sec Defocus = -2.167 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.41.14_batch_tomo_05_9_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 112.7 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.083 -0.267 IS units 804.3 26.4 unbinned pixels; need stage -0.034 0.315 if reset Tilting to 28 Correcting tilt backlash -5.9 -5.6 unbinned pixels; 1.2 2.6 nm along two shift axes; 2.1 -1.9 nm on specimen axes Rate = 14.32532 A/sec -2.6 4.7 unbinned pixels; 1.9 0.3 nm along two shift axes; -1.6 -1.1 nm on specimen axes Rate = 9.49068 A/sec Drift is low enough after shot 2 Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.16 nm/sec Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.17 nm/sec Defocus = -2.229 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.42.19_batch_tomo_05_9_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 118.2 In SetExposureForMean 120, change by a factor of 1.0207 would require too small a change in exposure time Image shift 0.139 -0.414 IS units 1257.1 16.0 unbinned pixels; need stage -0.044 0.503 if reset Tilting to -48 -48.00 degrees 19.9 -14.8 unbinned pixels; 7.2 4.8 nm along two shift axes; 4.6 7.4 nm on specimen axes Rate = 43.45802 A/sec -0.2 4.4 unbinned pixels; 1.5 0.5 nm along two shift axes; -1.5 -0.2 nm on specimen axes Rate = 7.70011 A/sec Drift is low enough after shot 2 Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.16 nm/sec Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.12 nm/sec Defocus = 0.322 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.43.27_batch_tomo_05_9_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 127.2 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.318 -0.818 IS units 2529.7 -86.5 unbinned pixels; need stage -0.048 1.341 if reset Tilting to -50 8.2 -4.3 unbinned pixels; 2.4 2.2 nm along two shift axes; 1.3 3.0 nm on specimen axes Rate = 16.18283 A/sec 1.5 2.1 unbinned pixels; 0.5 0.8 nm along two shift axes; -0.8 0.5 nm on specimen axes Rate = 4.61015 A/sec Drift is low enough after shot 2 Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.11 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.21 nm/sec Defocus = 0.397 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.44.30_batch_tomo_05_9_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 115.2 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.332 -0.823 IS units 2557.1 -121.2 unbinned pixels; need stage -0.037 1.413 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -14.4 2.9 unbinned pixels; 2.7 4.4 nm along two shift axes; -0.6 -5.1 nm on specimen axes Rate = 25.78298 A/sec -8.0 4.2 unbinned pixels; 2.3 2.2 nm along two shift axes; -1.2 -2.9 nm on specimen axes Rate = 15.85149 A/sec -8.5 1.9 unbinned pixels; 1.6 2.6 nm along two shift axes; -0.4 -3.0 nm on specimen axes Rate = 15.17744 A/sec -7.5 1.4 unbinned pixels; 1.3 2.3 nm along two shift axes; -0.3 -2.7 nm on specimen axes Rate = 13.41417 A/sec Drift is low enough after shot 4 Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.20 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.23 nm/sec Defocus = -2.269 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.45.56_batch_tomo_05_9_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 114.1 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.137 -0.381 IS units 1165.9 -10.6 unbinned pixels; need stage -0.032 0.476 if reset Tilting to 32 Correcting tilt backlash -7.6 -5.1 unbinned pixels; 0.8 3.1 nm along two shift axes; 2.0 -2.5 nm on specimen axes Rate = 16.12376 A/sec -0.3 3.6 unbinned pixels; 1.2 0.3 nm along two shift axes; -1.2 -0.2 nm on specimen axes Rate = 6.30379 A/sec Drift is low enough after shot 2 Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.24 nm/sec Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.12 nm/sec Defocus = -2.227 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.47.01_batch_tomo_05_9_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 118.0 In SetExposureForMean 120, change by a factor of 1.0247 would require too small a change in exposure time Image shift 0.177 -0.468 IS units 1441.3 -35.5 unbinned pixels; need stage -0.032 0.602 if reset Tilting to -52 -52.00 degrees 14.2 -7.7 unbinned pixels; 4.2 3.8 nm along two shift axes; 2.3 5.2 nm on specimen axes Rate = 28.27325 A/sec 6.1 0.8 unbinned pixels; 0.5 2.1 nm along two shift axes; -0.5 2.1 nm on specimen axes Rate = 10.8211 A/sec Drift is low enough after shot 2 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.21 nm/sec Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.13 nm/sec Defocus = 0.501 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.48.14_batch_tomo_05_9_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 130.1 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.387 -0.916 IS units 2862.2 -182.6 unbinned pixels; need stage -0.025 1.653 if reset Tilting to -54 4.7 -6.7 unbinned pixels; 2.8 0.7 nm along two shift axes; 2.2 1.8 nm on specimen axes Rate = 14.34673 A/sec 6.4 2.8 unbinned pixels; 0.2 2.4 nm along two shift axes; -1.2 2.1 nm on specimen axes Rate = 12.22157 A/sec Drift is low enough after shot 2 Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.09 nm/sec Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.14 nm/sec Defocus = 0.528 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.49.17_batch_tomo_05_9_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 117.6 In SetExposureForMean 120, change by a factor of 1.0214 would require too small a change in exposure time Image shift 0.409 -0.956 IS units 2994.0 -204.4 unbinned pixels; need stage -0.022 1.812 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -19.0 3.6 unbinned pixels; 3.4 5.8 nm along two shift axes; -0.7 -6.8 nm on specimen axes Rate = 33.87525 A/sec -9.1 3.3 unbinned pixels; 2.2 2.6 nm along two shift axes; -0.9 -3.3 nm on specimen axes Rate = 16.93939 A/sec -8.0 2.2 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.5 -2.9 nm on specimen axes Rate = 14.47801 A/sec -6.8 1.8 unbinned pixels; 1.4 2.0 nm along two shift axes; -0.4 -2.4 nm on specimen axes Rate = 12.30908 A/sec Drift is low enough after shot 4 Measured defocus = -3.85 microns changed by -0.15 to target drift = 0.19 nm/sec Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.40 nm/sec Defocus = -2.313 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.50.39_batch_tomo_05_9_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 105.5 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift 0.163 -0.424 IS units 1310.6 -40.3 unbinned pixels; need stage -0.026 0.561 if reset Tilting to 36 Correcting tilt backlash -14.1 -5.6 unbinned pixels; 0.2 5.3 nm along two shift axes; 2.4 -4.8 nm on specimen axes Rate = 26.74679 A/sec -1.4 1.7 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.6 -0.6 nm on specimen axes Rate = 3.91307 A/sec Drift is low enough after shot 2 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.25 nm/sec Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.28 nm/sec Defocus = -2.328 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.51.43_batch_tomo_05_9_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 115.3 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.190 -0.501 IS units 1545.9 -41.3 unbinned pixels; need stage -0.033 0.677 if reset Tilting to -56 -56.00 degrees 26.0 -12.2 unbinned pixels; 7.1 7.2 nm along two shift axes; 3.5 9.5 nm on specimen axes Rate = 50.33895 A/sec 5.6 0.7 unbinned pixels; 0.4 1.9 nm along two shift axes; -0.4 1.9 nm on specimen axes Rate = 9.90285 A/sec Drift is low enough after shot 2 Measured defocus = -4.12 microns changed by 0.12 to target drift = 0.18 nm/sec Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.43 nm/sec Defocus = 0.702 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.52.52_batch_tomo_05_9_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 137.6 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.463 -1.049 IS units 3297.9 -262.2 unbinned pixels; need stage -0.011 2.100 if reset Tilting to -58 8.8 -5.7 unbinned pixels; 2.9 2.2 nm along two shift axes; 1.7 3.3 nm on specimen axes Rate = 18.35594 A/sec -0.2 4.2 unbinned pixels; 1.4 0.4 nm along two shift axes; -1.5 -0.2 nm on specimen axes Rate = 7.3753 A/sec Drift is low enough after shot 2 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.21 nm/sec Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.29 nm/sec Defocus = 0.806 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.53.55_batch_tomo_05_9_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 116.8 In SetExposureForMean 120, change by a factor of 1.0241 would require too small a change in exposure time Image shift 0.478 -1.068 IS units 3364.9 -283.6 unbinned pixels; need stage -0.006 2.263 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -23.4 1.3 unbinned pixels; 3.2 7.6 nm along two shift axes; 0.3 -8.3 nm on specimen axes Rate = 41.17349 A/sec -9.0 3.9 unbinned pixels; 2.3 2.5 nm along two shift axes; -1.1 -3.3 nm on specimen axes Rate = 17.20617 A/sec -6.4 1.7 unbinned pixels; 1.3 1.9 nm along two shift axes; -0.4 -2.3 nm on specimen axes Rate = 11.67824 A/sec Drift is low enough after shot 3 Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.14 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.22 nm/sec Defocus = -2.421 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.55.12_batch_tomo_05_9_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 102.8 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.210 -0.490 IS units 1533.8 -105.5 unbinned pixels; need stage -0.011 0.693 if reset Tilting to 40 Correcting tilt backlash Trimming A by 30 30, reference by 20 20 -6.8 -7.8 unbinned pixels; 1.8 3.2 nm along two shift axes; 3.0 -2.2 nm on specimen axes Rate = 18.32818 A/sec -5.2 -0.0 unbinned pixels; 0.6 1.7 nm along two shift axes; 0.2 -1.8 nm on specimen axes Rate = 9.15175 A/sec Drift is low enough after shot 2 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.02 nm/sec Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.14 nm/sec Defocus = -2.413 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.56.17_batch_tomo_05_9_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 117.1 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.256 -0.598 IS units 1873.6 -127.2 unbinned pixels; need stage -0.014 0.870 if reset Tilting to -60 -60.00 degrees 23.0 -10.1 unbinned pixels; 6.1 6.4 nm along two shift axes; 2.8 8.4 nm on specimen axes Rate = 44.05456 A/sec 10.2 0.8 unbinned pixels; 0.9 3.5 nm along two shift axes; -0.6 3.5 nm on specimen axes Rate = 17.90303 A/sec 6.1 -1.9 unbinned pixels; 1.3 1.8 nm along two shift axes; 0.5 2.2 nm on specimen axes Rate = 11.1297 A/sec Drift is low enough after shot 3 Measured defocus = -4.11 microns changed by 0.11 to target drift = 0.20 nm/sec Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.23 nm/sec Defocus = 0.833 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.57.32_batch_tomo_05_9_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 141.9 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift 0.519 -1.144 IS units 3610.1 -325.3 unbinned pixels; need stage 0.001 2.574 if reset Tilting to -62 12.4 -8.1 unbinned pixels; 4.1 3.1 nm along two shift axes; 2.5 4.6 nm on specimen axes Rate = 25.92006 A/sec 7.3 2.4 unbinned pixels; 0.1 2.7 nm along two shift axes; -1.1 2.5 nm on specimen axes Rate = 13.55851 A/sec Drift is low enough after shot 2 Measured defocus = -4.14 microns changed by 0.14 to target drift = 0.22 nm/sec Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.07 nm/sec Defocus = 1.002 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_21.58.35_batch_tomo_05_9_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 114.3 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.543 -1.173 IS units 3714.1 -362.0 unbinned pixels; need stage 0.010 2.822 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -23.9 6.4 unbinned pixels; 4.9 7.2 nm along two shift axes; -1.5 -8.6 nm on specimen axes Rate = 43.4316 A/sec -8.4 1.5 unbinned pixels; 1.5 2.6 nm along two shift axes; -0.3 -3.0 nm on specimen axes Rate = 15.02651 A/sec -8.6 0.9 unbinned pixels; 1.3 2.7 nm along two shift axes; -0.0 -3.0 nm on specimen axes Rate = 15.08967 A/sec -5.1 1.2 unbinned pixels; 1.0 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 9.14066 A/sec Drift is low enough after shot 4 Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.47 nm/sec Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.30 nm/sec Defocus = -2.442 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.00.04_batch_tomo_05_9_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 103.2 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.275 -0.597 IS units 1887.2 -181.0 unbinned pixels; need stage 0.004 0.906 if reset Tilting to 44 Correcting tilt backlash Trimming A by 40 40, reference by 20 20 -6.7 -7.4 unbinned pixels; 1.7 3.1 nm along two shift axes; 2.8 -2.1 nm on specimen axes Rate = 17.75564 A/sec 0.5 2.1 unbinned pixels; 0.6 0.4 nm along two shift axes; -0.7 0.1 nm on specimen axes Rate = 3.72271 A/sec Drift is low enough after shot 2 Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.20 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.478 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.01.09_batch_tomo_05_9_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 113.9 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.314 -0.694 IS units 2188.2 -194.2 unbinned pixels; need stage -0.000 1.084 if reset Tilting to -64 -64.00 degrees 19.8 -9.2 unbinned pixels; 5.4 5.5 nm along two shift axes; 2.6 7.2 nm on specimen axes Rate = 38.28519 A/sec 12.5 0.4 unbinned pixels; 1.3 4.2 nm along two shift axes; -0.5 4.4 nm on specimen axes Rate = 21.96074 A/sec 5.9 -0.4 unbinned pixels; 0.8 1.9 nm along two shift axes; -0.0 2.1 nm on specimen axes Rate = 10.28455 A/sec Drift is low enough after shot 3 Measured defocus = -4.14 microns changed by 0.14 to target drift = 0.10 nm/sec Measured defocus = -3.87 microns changed by -0.13 to target drift = 0.33 nm/sec Defocus = 1.008 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.02.25_batch_tomo_05_9_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 151.1 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.581 -1.258 IS units 3980.1 -385.1 unbinned pixels; need stage 0.010 3.239 if reset Tilting to -66 8.7 -6.3 unbinned pixels; 3.1 2.1 nm along two shift axes; 2.0 3.2 nm on specimen axes Rate = 18.82511 A/sec 7.6 2.3 unbinned pixels; 0.1 2.8 nm along two shift axes; -1.1 2.6 nm on specimen axes Rate = 14.03658 A/sec 4.3 0.9 unbinned pixels; 0.2 1.5 nm along two shift axes; -0.5 1.5 nm on specimen axes Rate = 7.76149 A/sec Drift is low enough after shot 3 Measured defocus = -4.23 microns changed by 0.23 to target drift = 0.14 nm/sec Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.39 nm/sec Defocus = 1.153 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.03.40_batch_tomo_05_9_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 110.4 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.618 -1.304 IS units 4141.6 -442.1 unbinned pixels; need stage 0.025 3.636 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees Trimming A by 25 30, reference by 20 20 Trimming A by 30 30, reference by 20 20 -15.8 3.1 unbinned pixels; 2.9 4.9 nm along two shift axes; -0.6 -5.6 nm on specimen axes Rate = 28.22299 A/sec Trimming A by 30 30, reference by 20 20 -5.6 4.1 unbinned pixels; 2.0 1.4 nm along two shift axes; -1.3 -2.1 nm on specimen axes Rate = 12.10378 A/sec Drift is low enough after shot 2 Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.33 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.18 nm/sec Defocus = -2.508 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.04.52_batch_tomo_05_9_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 93.7 In SetExposureForMean 120, exposure time changed to 2.8800 Image shift 0.321 -0.679 IS units 2156.5 -227.8 unbinned pixels; need stage 0.012 1.108 if reset Tilting to 48 Correcting tilt backlash Trimming A by 65 65, reference by 25 30 Trimming A by 40 40, reference by 25 30 -6.0 -8.1 unbinned pixels; 2.0 3.0 nm along two shift axes; 3.0 -1.9 nm on specimen axes Rate = 17.80445 A/sec Trimming A by 40 45, reference by 25 30 -2.5 1.0 unbinned pixels; 0.6 0.7 nm along two shift axes; -0.3 -0.9 nm on specimen axes Rate = 4.66733 A/sec Drift is low enough after shot 2 Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.35 nm/sec Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.01 nm/sec Defocus = -2.464 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.06.02_batch_tomo_05_9_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 114.5 In SetExposureForMean 120, exposure time changed to 3.2400 Image shift 0.365 -0.770 IS units 2446.4 -260.9 unbinned pixels; need stage 0.015 1.305 if reset Tilting to -68 -68.00 degrees 9.1 -9.5 unbinned pixels; 4.2 1.9 nm along two shift axes; 3.1 3.5 nm on specimen axes Rate = 23.07941 A/sec 30.0 -1.5 unbinned pixels; 4.0 9.8 nm along two shift axes; -0.4 10.6 nm on specimen axes Rate = 52.66307 A/sec 5.0 -1.1 unbinned pixels; 1.0 1.5 nm along two shift axes; 0.2 1.8 nm on specimen axes Rate = 8.91153 A/sec Drift is low enough after shot 3 Measured defocus = -4.12 microns changed by 0.12 to target drift = 0.22 nm/sec Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.29 nm/sec Defocus = 1.309 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.07.19_batch_tomo_05_9_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 166.7 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.680 -1.406 IS units 4479.2 -512.5 unbinned pixels; need stage 0.039 4.272 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 0.22 microns changed by -4.22 to target drift = 0.46 nm/sec Focus area changed from stored state, axis position 2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_06.st Running script 1 at item 11 (-380.51, -264.65) 22:08:16 Refine ZLP: additional offset = -0.6 eV; total offset = -13.1 eV Item 11: Stage: -380.51 -264.65 -223 Label: 11 Note: Sec 10 - anchor_maps.map Target defocus is: -4.00 um Target defocus is: -4.25 um Target defocus = -4.25 Trimming A by 21 135, reference by 24 135 Angle = -4.40, increment = 0.60, shift = 1.0 -8.1 pixels, 0.000 um Y Trimming A by 21 135, reference by 24 135 Angle = -3.20, increment = 1.80, shift = -38.3 -16.5 pixels, -0.091 um Y Trimming A by 26 135, reference by 24 135 Angle = 0.41, increment = 5.41, shift = -158.8 -32.7 pixels, -0.369 um Y Trimming A by 26 135, reference by 24 135 Angle = 3.00, increment = 8.00, shift = -226.1 -46.9 pixels, -0.525 um Y Rough eucentricity: changing Z by 3.76 to -219.61, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 23 135, reference by 21 135 Angle = 18.00, shift = -46.0, -3.4 pixels, 0.004, -0.105 um, IS = -0.004, 0.105 Trimming A by 24 135, reference by 23 135 Angle = 11.00, shift = -32.6, -3.8 pixels, -0.000, -0.075 um, IS = -0.004, 0.180 Trimming A by 24 135, reference by 24 135 Angle = 4.00, shift = -6.4, 1.7 pixels, 0.006, -0.014 um, IS = -0.009, 0.194 Trimming A by 24 55 130 20, reference by 21 36 130 20 Angle = -4.00, shift = -5.5, -24.2 pixels, -0.053, -0.019 um, IS = 0.044, 0.213 Trimming A by 24 135, reference by 21 135 Angle = -11.00, shift = -33.9, -10.2 pixels, -0.014, -0.080 um, IS = 0.059, 0.292 Trimming A by 29 130, reference by 24 135 Angle = -18.00, shift = -33.9, -0.9 pixels, 0.007, -0.077 um, IS = 0.052, 0.370 Trimming A by 29 130, reference by 29 130 Angle = -24.00, shift = -1.9, 0.6 pixels, 0.002, -0.004 um, IS = 0.050, 0.374 General solution delZ 0.42, delY 0.05, yZero 0.23 solution with assumed YZero 0.20: delZ 0.42, delY -0.28 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.42 microns and lateral displacement of -0.05 microns Trimming A by 21 31 125 20, reference by 21 37 125 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.56 Aligning to target with image shift equivalent to stage shift: 0.01 -0.56 Disparity in stage position after aligning to map item itself: -0.01 -0.54 (align shift -0.03 0.00) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -380.50 -264.62 -220.02 -4.7 -0.8 unbinned pixels; 0.3 1.6 nm along two shift axes; 0.4 -1.6 nm on specimen axes Rate = 8.35059 A/sec Drift is low enough after shot 1 Measured defocus = 0.74 microns changed by -4.99 to target drift = 0.05 nm/sec Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.03 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -1.087 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.13.05_batch_tomo_06_11_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 84.0 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift -0.235 0.486 IS units -1547.6 178.5 unbinned pixels; need stage -0.014 -0.562 if reset Tilting to -8 Correcting tilt backlash 11.3 -0.4 unbinned pixels; 1.5 3.7 nm along two shift axes; -0.2 4.0 nm on specimen axes Rate = 19.91309 A/sec 5.9 0.4 unbinned pixels; 0.6 2.0 nm along two shift axes; -0.3 2.1 nm on specimen axes Rate = 10.40452 A/sec Drift is low enough after shot 2 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -1.194 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.14.09_batch_tomo_06_11_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0224 would require too small a change in exposure time Image shift -0.264 0.461 IS units -1508.3 280.9 unbinned pixels; need stage -0.050 -0.548 if reset Tilting to -12 -12.00 degrees -10.2 1.2 unbinned pixels; 1.6 3.2 nm along two shift axes; -0.1 -3.6 nm on specimen axes Rate = 17.94213 A/sec -3.6 5.2 unbinned pixels; 2.1 0.6 nm along two shift axes; -1.7 -1.4 nm on specimen axes Rate = 11.13025 A/sec Drift is low enough after shot 2 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.10 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -1.010 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.15.13_batch_tomo_06_11_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0214 would require too small a change in exposure time Image shift -0.329 0.585 IS units -1909.6 338.4 unbinned pixels; need stage -0.058 -0.702 if reset Tilting to -14 9.6 -0.4 unbinned pixels; 1.3 3.1 nm along two shift axes; -0.2 3.4 nm on specimen axes Rate = 16.94071 A/sec 6.7 1.8 unbinned pixels; 0.2 2.4 nm along two shift axes; -0.8 2.3 nm on specimen axes Rate = 12.12609 A/sec Drift is low enough after shot 2 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.09 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -0.915 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.16.16_batch_tomo_06_11_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 117.2 In SetExposureForMean 120, change by a factor of 1.0244 would require too small a change in exposure time Image shift -0.389 0.701 IS units -2282.6 390.6 unbinned pixels; need stage -0.064 -0.845 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 11.0 -0.4 unbinned pixels; 1.4 3.6 nm along two shift axes; -0.2 3.9 nm on specimen axes Rate = 19.30597 A/sec 6.8 -0.7 unbinned pixels; 1.0 2.2 nm along two shift axes; 0.0 2.4 nm on specimen axes Rate = 12.04574 A/sec Drift is low enough after shot 2 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.16 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -1.258 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.17.22_batch_tomo_06_11_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0226 would require too small a change in exposure time Image shift -0.248 0.422 IS units -1388.9 273.1 unbinned pixels; need stage -0.051 -0.503 if reset Tilting to -4 Correcting tilt backlash -7.3 1.0 unbinned pixels; 1.2 2.3 nm along two shift axes; -0.1 -2.6 nm on specimen axes Rate = 12.87956 A/sec Drift is low enough after shot 1 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -1.327 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.18.21_batch_tomo_06_11_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 117.1 In SetExposureForMean 120, change by a factor of 1.0251 would require too small a change in exposure time Image shift -0.261 0.453 IS units -1484.3 280.3 unbinned pixels; need stage -0.051 -0.535 if reset Tilting to -16 -16.00 degrees -7.1 1.5 unbinned pixels; 1.3 2.2 nm along two shift axes; -0.3 -2.5 nm on specimen axes Rate = 12.65945 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.04 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -0.805 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.19.25_batch_tomo_06_11_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 116.9 In SetExposureForMean 120, change by a factor of 1.0267 would require too small a change in exposure time Image shift -0.404 0.705 IS units -2308.2 429.8 unbinned pixels; need stage -0.077 -0.864 if reset Tilting to -18 13.2 -7.0 unbinned pixels; 3.9 3.5 nm along two shift axes; 2.0 4.9 nm on specimen axes Rate = 26.20652 A/sec 10.2 1.2 unbinned pixels; 0.8 3.5 nm along two shift axes; -0.7 3.5 nm on specimen axes Rate = 18.06353 A/sec 6.2 -0.3 unbinned pixels; 0.8 2.0 nm along two shift axes; -0.1 2.2 nm on specimen axes Rate = 10.82885 A/sec Drift is low enough after shot 3 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.11 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -0.714 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.20.33_batch_tomo_06_11_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 116.5 In SetExposureForMean 120, change by a factor of 1.0308 would require too small a change in exposure time Image shift -0.372 0.649 IS units -2124.2 396.3 unbinned pixels; need stage -0.071 -0.803 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 10.3 -0.1 unbinned pixels; 1.2 3.4 nm along two shift axes; -0.3 3.6 nm on specimen axes Rate = 18.03356 A/sec 7.7 1.4 unbinned pixels; 0.4 2.7 nm along two shift axes; -0.7 2.7 nm on specimen axes Rate = 13.8297 A/sec Drift is low enough after shot 2 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -1.416 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.21.40_batch_tomo_06_11_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 116.6 In SetExposureForMean 120, change by a factor of 1.0292 would require too small a change in exposure time Image shift -0.293 0.481 IS units -1592.1 338.4 unbinned pixels; need stage -0.067 -0.574 if reset Tilting to 0 Correcting tilt backlash -3.9 -0.5 unbinned pixels; 0.3 1.4 nm along two shift axes; 0.3 -1.4 nm on specimen axes Rate = 6.90743 A/sec Drift is low enough after shot 1 Measured defocus = -4.14 microns changed by -0.11 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -1.525 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.22.39_batch_tomo_06_11_009_0.0.tif Saved Z = 9, 0.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, change by a factor of 1.0322 would require too small a change in exposure time Image shift -0.276 0.475 IS units -1559.7 299.8 unbinned pixels; need stage -0.055 -0.561 if reset Tilting to -20 -20.00 degrees -6.1 -0.6 unbinned pixels; 0.5 2.1 nm along two shift axes; 0.4 -2.1 nm on specimen axes Rate = 10.80472 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.04 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -0.607 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.23.38_batch_tomo_06_11_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, change by a factor of 1.0346 would require too small a change in exposure time Image shift -0.378 0.622 IS units -2057.0 437.1 unbinned pixels; need stage -0.087 -0.789 if reset Tilting to -22 13.0 -7.7 unbinned pixels; 4.1 3.4 nm along two shift axes; 2.3 4.8 nm on specimen axes Rate = 26.48925 A/sec 9.5 1.3 unbinned pixels; 0.7 3.3 nm along two shift axes; -0.8 3.3 nm on specimen axes Rate = 16.92718 A/sec 5.8 0.1 unbinned pixels; 0.6 1.9 nm along two shift axes; -0.2 2.0 nm on specimen axes Rate = 10.21966 A/sec Drift is low enough after shot 3 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.11 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -0.542 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.24.47_batch_tomo_06_11_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 115.0 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift -0.342 0.562 IS units -1860.0 395.8 unbinned pixels; need stage -0.079 -0.723 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 7.9 1.1 unbinned pixels; 0.6 2.8 nm along two shift axes; -0.6 2.7 nm on specimen axes Rate = 14.06688 A/sec 7.1 3.0 unbinned pixels; 0.2 2.7 nm along two shift axes; -1.3 2.4 nm on specimen axes Rate = 13.57042 A/sec Drift is low enough after shot 2 Measured defocus = -4.15 microns changed by -0.10 to target drift = 0.06 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -1.617 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.25.55_batch_tomo_06_11_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 123.7 In SetExposureForMean 120, change by a factor of 0.9700 would require too small a change in exposure time Image shift -0.327 0.536 IS units -1774.8 379.5 unbinned pixels; need stage -0.076 -0.640 if reset Tilting to 4 Correcting tilt backlash -5.9 -2.0 unbinned pixels; 0.0 2.2 nm along two shift axes; 0.9 -2.0 nm on specimen axes Rate = 11.03053 A/sec Drift is low enough after shot 1 Measured defocus = -4.12 microns changed by -0.13 to target drift = 0.12 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.10 nm/sec Defocus = -1.729 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.26.59_batch_tomo_06_11_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 123.1 In SetExposureForMean 120, change by a factor of 0.9751 would require too small a change in exposure time Image shift -0.298 0.503 IS units -1656.3 331.8 unbinned pixels; need stage -0.063 -0.598 if reset Tilting to -24 -24.00 degrees -5.3 -1.6 unbinned pixels; 0.1 1.9 nm along two shift axes; 0.7 -1.8 nm on specimen axes Rate = 9.71307 A/sec Drift is low enough after shot 1 Measured defocus = -4.36 microns changed by 0.11 to target drift = 0.03 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = -0.413 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.27.59_batch_tomo_06_11_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 122.5 In SetExposureForMean 120, change by a factor of 0.9807 would require too small a change in exposure time Image shift -0.336 0.507 IS units -1704.1 432.6 unbinned pixels; need stage -0.096 -0.675 if reset Tilting to -26 10.9 -6.5 unbinned pixels; 3.4 2.8 nm along two shift axes; 2.0 4.0 nm on specimen axes Rate = 22.24667 A/sec 7.9 1.2 unbinned pixels; 0.5 2.8 nm along two shift axes; -0.7 2.7 nm on specimen axes Rate = 14.07498 A/sec 5.5 0.3 unbinned pixels; 0.6 1.9 nm along two shift axes; -0.3 1.9 nm on specimen axes Rate = 9.66796 A/sec Drift is low enough after shot 3 Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.10 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -0.372 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.29.07_batch_tomo_06_11_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 121.3 In SetExposureForMean 120, change by a factor of 0.9904 would require too small a change in exposure time Image shift -0.310 0.466 IS units -1568.3 399.5 unbinned pixels; need stage -0.089 -0.631 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 14.5 2.2 unbinned pixels; 1.0 5.1 nm along two shift axes; -1.2 5.0 nm on specimen axes Rate = 25.71064 A/sec 7.6 3.5 unbinned pixels; 0.3 2.9 nm along two shift axes; -1.5 2.5 nm on specimen axes Rate = 14.70439 A/sec 2.8 1.9 unbinned pixels; 0.3 1.1 nm along two shift axes; -0.7 0.9 nm on specimen axes Rate = 5.88098 A/sec Drift is low enough after shot 3 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.04 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -1.843 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.30.25_batch_tomo_06_11_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 121.9 In SetExposureForMean 120, change by a factor of 0.9848 would require too small a change in exposure time Image shift -0.352 0.565 IS units -1877.6 419.9 unbinned pixels; need stage -0.087 -0.681 if reset Tilting to 8 Correcting tilt backlash -9.0 0.7 unbinned pixels; 1.3 2.9 nm along two shift axes; 0.0 -3.2 nm on specimen axes Rate = 15.74936 A/sec -9.2 5.1 unbinned pixels; 2.8 2.4 nm along two shift axes; -1.5 -3.4 nm on specimen axes Rate = 18.43098 A/sec -5.5 2.0 unbinned pixels; 1.3 1.6 nm along two shift axes; -0.5 -2.0 nm on specimen axes Rate = 10.20035 A/sec Drift is low enough after shot 3 Measured defocus = -4.11 microns changed by -0.14 to target drift = 0.13 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.09 nm/sec Defocus = -1.976 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.31.36_batch_tomo_06_11_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 120.6 In SetExposureForMean 120, change by a factor of 0.9948 would require too small a change in exposure time Image shift -0.335 0.550 IS units -1819.3 389.5 unbinned pixels; need stage -0.078 -0.662 if reset Tilting to -28 -28.00 degrees -1.0 -5.8 unbinned pixels; 1.8 1.0 nm along two shift axes; 2.1 -0.2 nm on specimen axes Rate = 10.49162 A/sec Drift is low enough after shot 1 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.03 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -0.292 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.32.37_batch_tomo_06_11_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 119.9 In SetExposureForMean 120, change by a factor of 1.0026 would require too small a change in exposure time Image shift -0.288 0.379 IS units -1310.4 422.2 unbinned pixels; need stage -0.105 -0.540 if reset Tilting to -30 8.9 -8.4 unbinned pixels; 3.8 2.0 nm along two shift axes; 2.7 3.4 nm on specimen axes Rate = 21.52594 A/sec 10.0 1.4 unbinned pixels; 0.7 3.5 nm along two shift axes; -0.8 3.5 nm on specimen axes Rate = 17.77503 A/sec 5.1 -0.5 unbinned pixels; 0.8 1.6 nm along two shift axes; 0.0 1.8 nm on specimen axes Rate = 9.04667 A/sec Drift is low enough after shot 3 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.10 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -0.203 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.33.46_batch_tomo_06_11_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 118.2 In SetExposureForMean 120, change by a factor of 1.0169 would require too small a change in exposure time Image shift -0.246 0.309 IS units -1079.5 376.5 unbinned pixels; need stage -0.096 -0.455 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -3.8 1.3 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 7.04726 A/sec Drift is low enough after shot 1 Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.07 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.066 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.34.48_batch_tomo_06_11_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 119.4 In SetExposureForMean 120, change by a factor of 1.0055 would require too small a change in exposure time Image shift -0.368 0.578 IS units -1928.3 451.3 unbinned pixels; need stage -0.096 -0.707 if reset Tilting to 12 Correcting tilt backlash -6.6 -1.4 unbinned pixels; 0.3 2.3 nm along two shift axes; 0.7 -2.3 nm on specimen axes Rate = 11.80374 A/sec Drift is low enough after shot 1 Measured defocus = -4.11 microns changed by -0.14 to target drift = 0.10 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -2.196 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.35.47_batch_tomo_06_11_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0218 would require too small a change in exposure time Image shift -0.341 0.540 IS units -1797.6 414.1 unbinned pixels; need stage -0.087 -0.664 if reset Tilting to -32 -32.00 degrees -0.4 -6.4 unbinned pixels; 2.1 0.9 nm along two shift axes; 2.2 0.1 nm on specimen axes Rate = 11.26094 A/sec Drift is low enough after shot 1 Measured defocus = -4.36 microns changed by 0.11 to target drift = 0.03 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.080 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.36.48_batch_tomo_06_11_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 116.7 In SetExposureForMean 120, change by a factor of 1.0306 would require too small a change in exposure time Image shift -0.212 0.202 IS units -756.0 384.6 unbinned pixels; need stage -0.109 -0.330 if reset Tilting to -34 13.7 -8.3 unbinned pixels; 4.3 3.5 nm along two shift axes; 2.5 5.0 nm on specimen axes Rate = 27.97565 A/sec 6.0 1.2 unbinned pixels; 0.3 2.1 nm along two shift axes; -0.6 2.1 nm on specimen axes Rate = 10.75698 A/sec Drift is low enough after shot 2 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.07 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = 0.000 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.37.56_batch_tomo_06_11_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 114.7 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift -0.171 0.130 IS units -521.2 342.8 unbinned pixels; need stage -0.102 -0.236 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -8.5 3.1 unbinned pixels; 2.0 2.4 nm along two shift axes; -0.8 -3.1 nm on specimen axes Rate = 15.77546 A/sec -1.2 1.8 unbinned pixels; 0.7 0.2 nm along two shift axes; -0.6 -0.5 nm on specimen axes Rate = 3.81441 A/sec Drift is low enough after shot 2 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.06 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -2.284 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.39.05_batch_tomo_06_11_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 123.7 In SetExposureForMean 120, change by a factor of 0.9707 would require too small a change in exposure time Image shift -0.366 0.578 IS units -1925.7 445.7 unbinned pixels; need stage -0.094 -0.717 if reset Tilting to 16 Correcting tilt backlash -6.0 -1.9 unbinned pixels; 0.1 2.2 nm along two shift axes; 0.9 -2.1 nm on specimen axes Rate = 11.14188 A/sec Drift is low enough after shot 1 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.07 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.380 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.40.05_batch_tomo_06_11_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 121.6 In SetExposureForMean 120, change by a factor of 0.9876 would require too small a change in exposure time Image shift -0.340 0.533 IS units -1779.5 417.4 unbinned pixels; need stage -0.089 -0.669 if reset Tilting to -36 -36.00 degrees 2.0 -6.7 unbinned pixels; 2.5 0.1 nm along two shift axes; 2.3 0.9 nm on specimen axes Rate = 12.30423 A/sec Drift is low enough after shot 1 Measured defocus = -4.35 microns changed by 0.10 to target drift = 0.07 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.02 nm/sec Defocus = 0.092 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.41.06_batch_tomo_06_11_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 120.0 In SetExposureForMean 120, change by a factor of 1.0022 would require too small a change in exposure time Image shift -0.126 0.011 IS units -153.4 332.7 unbinned pixels; need stage -0.110 -0.080 if reset Tilting to -38 7.2 -7.7 unbinned pixels; 3.4 1.4 nm along two shift axes; 2.5 2.7 nm on specimen axes Rate = 18.48536 A/sec 7.5 1.8 unbinned pixels; 0.3 2.7 nm along two shift axes; -0.9 2.6 nm on specimen axes Rate = 13.5594 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.11 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.09 nm/sec Defocus = 0.165 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.42.09_batch_tomo_06_11_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 117.6 In SetExposureForMean 120, change by a factor of 1.0223 would require too small a change in exposure time Image shift -0.080 -0.068 IS units 105.3 283.3 unbinned pixels; need stage -0.101 0.036 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -7.6 1.4 unbinned pixels; 1.3 2.3 nm along two shift axes; -0.3 -2.7 nm on specimen axes Rate = 13.46196 A/sec Drift is low enough after shot 1 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.18 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.14 nm/sec Defocus = -2.488 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.43.13_batch_tomo_06_11_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 119.8 In SetExposureForMean 120, change by a factor of 1.0024 would require too small a change in exposure time Image shift -0.368 0.586 IS units -1949.4 445.5 unbinned pixels; need stage -0.093 -0.740 if reset Tilting to 20 Correcting tilt backlash -2.5 -0.9 unbinned pixels; 0.0 0.9 nm along two shift axes; 0.4 -0.9 nm on specimen axes Rate = 4.73004 A/sec Drift is low enough after shot 1 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.07 nm/sec Defocus = -2.562 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.44.13_batch_tomo_06_11_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 117.2 In SetExposureForMean 120, change by a factor of 1.0250 would require too small a change in exposure time Image shift -0.337 0.492 IS units -1663.3 448.6 unbinned pixels; need stage -0.103 -0.641 if reset Tilting to -40 -40.00 degrees 4.2 -8.0 unbinned pixels; 3.2 0.4 nm along two shift axes; 2.7 1.7 nm on specimen axes Rate = 15.92656 A/sec 3.3 2.3 unbinned pixels; 0.4 1.4 nm along two shift axes; -0.9 1.1 nm on specimen axes Rate = 7.07473 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.08 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.02 nm/sec Defocus = 0.198 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.45.22_batch_tomo_06_11_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 115.3 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift -0.017 -0.231 IS units 608.6 265.3 unbinned pixels; need stage -0.110 0.270 if reset Tilting to -42 5.3 -6.9 unbinned pixels; 2.9 0.9 nm along two shift axes; 2.3 2.1 nm on specimen axes Rate = 15.2818 A/sec 6.6 1.7 unbinned pixels; 0.2 2.4 nm along two shift axes; -0.8 2.3 nm on specimen axes Rate = 11.96125 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.09 nm/sec Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.07 nm/sec Defocus = 0.210 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.46.25_batch_tomo_06_11_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 119.4 In SetExposureForMean 120, change by a factor of 1.0074 would require too small a change in exposure time Image shift 0.019 -0.296 IS units 820.6 230.6 unbinned pixels; need stage -0.105 0.380 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -13.2 1.4 unbinned pixels; 2.0 4.2 nm along two shift axes; -0.1 -4.7 nm on specimen axes Rate = 23.29436 A/sec -6.2 1.9 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.2 nm on specimen axes Rate = 11.29602 A/sec Drift is low enough after shot 2 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.18 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.13 nm/sec Defocus = -2.649 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.47.34_batch_tomo_06_11_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 121.9 In SetExposureForMean 120, change by a factor of 0.9849 would require too small a change in exposure time Image shift -0.352 0.529 IS units -1779.7 454.5 unbinned pixels; need stage -0.102 -0.695 if reset Tilting to 24 Correcting tilt backlash -2.1 -1.1 unbinned pixels; 0.1 0.8 nm along two shift axes; 0.5 -0.7 nm on specimen axes Rate = 4.22372 A/sec Drift is low enough after shot 1 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.693 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.48.34_batch_tomo_06_11_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 118.9 In SetExposureForMean 120, change by a factor of 1.0101 would require too small a change in exposure time Image shift -0.320 0.424 IS units -1464.3 466.7 unbinned pixels; need stage -0.116 -0.583 if reset Tilting to -44 -44.00 degrees 4.0 -7.5 unbinned pixels; 3.0 0.4 nm along two shift axes; 2.5 1.6 nm on specimen axes Rate = 14.9783 A/sec 1.7 2.7 unbinned pixels; 0.7 0.9 nm along two shift axes; -1.0 0.5 nm on specimen axes Rate = 5.60419 A/sec Drift is low enough after shot 2 Measured defocus = -4.35 microns changed by 0.10 to target drift = 0.05 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = 0.315 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.49.42_batch_tomo_06_11_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.073 -0.440 IS units 1263.3 220.8 unbinned pixels; need stage -0.115 0.611 if reset Tilting to -46 4.4 -7.8 unbinned pixels; 3.1 0.5 nm along two shift axes; 2.6 1.8 nm on specimen axes Rate = 15.73096 A/sec 7.5 2.5 unbinned pixels; 0.0 2.8 nm along two shift axes; -1.1 2.5 nm on specimen axes Rate = 13.86373 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.06 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.06 nm/sec Defocus = 0.337 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.50.45_batch_tomo_06_11_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 119.6 In SetExposureForMean 120, change by a factor of 1.0064 would require too small a change in exposure time Image shift 0.113 -0.505 IS units 1481.1 172.2 unbinned pixels; need stage -0.105 0.746 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -12.4 3.7 unbinned pixels; 2.7 3.7 nm along two shift axes; -0.9 -4.5 nm on specimen axes Rate = 22.67856 A/sec -7.1 1.9 unbinned pixels; 1.5 2.1 nm along two shift axes; -0.5 -2.5 nm on specimen axes Rate = 12.80855 A/sec Drift is low enough after shot 2 Measured defocus = -4.14 microns changed by -0.11 to target drift = 0.14 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.12 nm/sec Defocus = -2.791 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.51.56_batch_tomo_06_11_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 122.4 In SetExposureForMean 120, change by a factor of 0.9822 would require too small a change in exposure time Image shift -0.347 0.489 IS units -1667.6 480.2 unbinned pixels; need stage -0.114 -0.673 if reset Tilting to 28 Correcting tilt backlash -3.5 -4.7 unbinned pixels; 1.2 1.7 nm along two shift axes; 1.7 -1.1 nm on specimen axes Rate = 10.30943 A/sec Drift is low enough after shot 1 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.06 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -2.834 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_22.52.56_batch_tomo_06_11_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 118.7 In SetExposureForMean 120, change by a factor of 1.0131 would require too small a change in exposure time Image shift -0.301 0.364 IS units -1282.9 473.2 unbinned pixels; need stage -0.123 -0.531 if reset Tilting to -48 -48.00 degrees 6.4 -7.2 unbinned pixels; 3.1 1.3 nm along two shift axes; 2.3 2.5 nm on specimen axes Rate = 16.89487 A/sec 2.7 2.7 unbinned pixels; 0.6 1.2 nm along two shift axes; -1.0 0.9 nm on specimen axes Rate = 6.81296 A/sec Drift is low enough after shot 2 Measured defocus = -4.35 microns changed by 0.10 to target drift = 0.05 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = 0.445 um Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.03.07_batch_tomo_06_11_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 116.0 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.139 -0.614 IS units 1799.5 204.5 unbinned pixels; need stage -0.126 0.941 if reset Tilting to -50 7.6 -0.8 unbinned pixels; 1.2 2.4 nm along two shift axes; 0.1 2.7 nm on specimen axes Rate = 13.32443 A/sec Drift is low enough after shot 1 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.07 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = 0.502 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.04.05_batch_tomo_06_11_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 117.9 In SetExposureForMean 120, change by a factor of 1.0225 would require too small a change in exposure time Image shift 0.150 -0.628 IS units 1849.8 189.9 unbinned pixels; need stage -0.122 1.008 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -17.6 2.3 unbinned pixels; 2.8 5.5 nm along two shift axes; -0.3 -6.2 nm on specimen axes Rate = 31.10427 A/sec -8.9 4.2 unbinned pixels; 2.4 2.4 nm along two shift axes; -1.2 -3.2 nm on specimen axes Rate = 17.15317 A/sec -6.0 2.3 unbinned pixels; 1.5 1.7 nm along two shift axes; -0.6 -2.2 nm on specimen axes Rate = 11.30259 A/sec Drift is low enough after shot 3 Measured defocus = -4.09 microns changed by -0.16 to target drift = 0.17 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -2.985 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.05.22_batch_tomo_06_11_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 122.3 In SetExposureForMean 120, change by a factor of 0.9830 would require too small a change in exposure time Image shift -0.320 0.398 IS units -1395.2 493.4 unbinned pixels; need stage -0.127 -0.588 if reset Tilting to 32 Correcting tilt backlash -15.9 -5.8 unbinned pixels; 0.1 6.0 nm along two shift axes; 2.5 -5.4 nm on specimen axes Rate = 29.82402 A/sec -4.1 2.6 unbinned pixels; 1.4 1.0 nm along two shift axes; -0.8 -1.5 nm on specimen axes Rate = 8.49019 A/sec Drift is low enough after shot 2 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.13 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.09 nm/sec Defocus = -3.019 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.06.38_batch_tomo_06_11_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 118.0 In SetExposureForMean 120, change by a factor of 1.0199 would require too small a change in exposure time Image shift -0.295 0.321 IS units -1159.3 498.4 unbinned pixels; need stage -0.136 -0.502 if reset Tilting to -52 -52.00 degrees 8.5 -7.2 unbinned pixels; 3.4 1.9 nm along two shift axes; 2.3 3.2 nm on specimen axes Rate = 19.44775 A/sec 5.2 1.2 unbinned pixels; 0.2 1.9 nm along two shift axes; -0.6 1.8 nm on specimen axes Rate = 9.33689 A/sec Drift is low enough after shot 2 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.11 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = 0.587 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.07.47_batch_tomo_06_11_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 113.3 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.200 -0.725 IS units 2161.2 145.2 unbinned pixels; need stage -0.116 1.234 if reset Tilting to -54 1.8 -5.8 unbinned pixels; 2.1 0.1 nm along two shift axes; 2.0 0.8 nm on specimen axes Rate = 10.74017 A/sec Drift is low enough after shot 1 Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.08 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = 0.611 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.08.45_batch_tomo_06_11_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 113.9 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift 0.228 -0.788 IS units 2360.4 128.4 unbinned pixels; need stage -0.117 1.413 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -17.0 0.7 unbinned pixels; 2.2 5.5 nm along two shift axes; 0.3 -6.0 nm on specimen axes Rate = 29.76895 A/sec -7.0 3.1 unbinned pixels; 1.8 2.0 nm along two shift axes; -0.9 -2.6 nm on specimen axes Rate = 13.3912 A/sec Drift is low enough after shot 2 Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.16 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.13 nm/sec Defocus = -3.123 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.09.57_batch_tomo_06_11_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 125.4 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift -0.289 0.293 IS units -1079.1 506.7 unbinned pixels; need stage -0.141 -0.480 if reset Tilting to 36 Correcting tilt backlash -6.2 -6.7 unbinned pixels; 1.5 2.9 nm along two shift axes; 2.5 -2.0 nm on specimen axes Rate = 16.15133 A/sec -1.6 1.7 unbinned pixels; 0.7 0.3 nm along two shift axes; -0.5 -0.6 nm on specimen axes Rate = 4.03209 A/sec Drift is low enough after shot 2 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.03 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -3.152 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.11.03_batch_tomo_06_11_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 114.0 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift -0.241 0.174 IS units -708.5 491.0 unbinned pixels; need stage -0.147 -0.329 if reset Tilting to -56 -56.00 degrees 8.0 -5.9 unbinned pixels; 2.9 1.9 nm along two shift axes; 1.8 3.0 nm on specimen axes Rate = 17.36684 A/sec 3.5 2.7 unbinned pixels; 0.5 1.5 nm along two shift axes; -1.1 1.1 nm on specimen axes Rate = 7.77784 A/sec Drift is low enough after shot 2 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.10 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = 0.720 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.12.13_batch_tomo_06_11_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 113.7 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.255 -0.866 IS units 2598.5 130.7 unbinned pixels; need stage -0.125 1.635 if reset Tilting to -58 2.7 -5.7 unbinned pixels; 2.2 0.2 nm along two shift axes; 1.9 1.1 nm on specimen axes Rate = 11.08325 A/sec Drift is low enough after shot 1 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.09 nm/sec Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.08 nm/sec Defocus = 0.761 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.13.11_batch_tomo_06_11_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 112.3 In SetExposureForMean 120, exposure time changed to 2.8800 Image shift 0.281 -0.926 IS units 2784.6 117.2 unbinned pixels; need stage -0.126 1.851 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -14.5 0.5 unbinned pixels; 1.8 4.7 nm along two shift axes; 0.3 -5.1 nm on specimen axes Rate = 25.46889 A/sec -5.1 2.0 unbinned pixels; 1.3 1.4 nm along two shift axes; -0.5 -1.8 nm on specimen axes Rate = 9.56935 A/sec Drift is low enough after shot 2 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.12 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.10 nm/sec Defocus = -3.273 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.14.28_batch_tomo_06_11_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 130.5 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift -0.253 0.207 IS units -810.4 490.2 unbinned pixels; need stage -0.144 -0.384 if reset Tilting to 40 Correcting tilt backlash -7.3 -7.7 unbinned pixels; 1.7 3.3 nm along two shift axes; 2.9 -2.3 nm on specimen axes Rate = 18.65999 A/sec -1.0 0.8 unbinned pixels; 0.4 0.2 nm along two shift axes; -0.2 -0.4 nm on specimen axes Rate = 2.25213 A/sec Drift is low enough after shot 2 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.02 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -3.306 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.15.34_batch_tomo_06_11_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 112.9 In SetExposureForMean 120, exposure time changed to 2.7600 Image shift -0.201 0.092 IS units -446.4 459.3 unbinned pixels; need stage -0.144 -0.225 if reset Tilting to -60 -60.00 degrees 9.8 -10.4 unbinned pixels; 4.6 2.0 nm along two shift axes; 3.3 3.7 nm on specimen axes Rate = 25.02046 A/sec 5.6 1.5 unbinned pixels; 0.2 2.0 nm along two shift axes; -0.7 1.9 nm on specimen axes Rate = 10.14326 A/sec Drift is low enough after shot 2 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.14 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.10 nm/sec Defocus = 0.821 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.16.45_batch_tomo_06_11_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 110.3 In SetExposureForMean 120, exposure time changed to 3.0000 Image shift 0.347 -1.062 IS units 3218.6 66.1 unbinned pixels; need stage -0.122 2.272 if reset Tilting to -62 5.0 -5.2 unbinned pixels; 2.3 1.0 nm along two shift axes; 1.7 1.9 nm on specimen axes Rate = 12.65173 A/sec Drift is low enough after shot 1 Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.05 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = 0.865 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.17.44_batch_tomo_06_11_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 111.6 In SetExposureForMean 120, exposure time changed to 3.2400 Image shift 0.379 -1.114 IS units 3390.6 29.6 unbinned pixels; need stage -0.115 2.552 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -14.9 0.0 unbinned pixels; 1.7 4.9 nm along two shift axes; 0.5 -5.2 nm on specimen axes Rate = 26.07439 A/sec -4.4 1.6 unbinned pixels; 1.1 1.3 nm along two shift axes; -0.4 -1.6 nm on specimen axes Rate = 8.23798 A/sec Drift is low enough after shot 2 Measured defocus = -4.12 microns changed by -0.13 to target drift = 0.10 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -3.432 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.18.56_batch_tomo_06_11_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 130.4 In SetExposureForMean 120, exposure time changed to 3.0000 Image shift -0.206 0.120 IS units -527.8 448.0 unbinned pixels; need stage -0.138 -0.271 if reset Tilting to 44 Correcting tilt backlash -6.1 -6.8 unbinned pixels; 1.6 2.8 nm along two shift axes; 2.6 -1.9 nm on specimen axes Rate = 16.16475 A/sec 0.7 0.8 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.3 0.2 nm on specimen axes Rate = 1.90854 A/sec Drift is low enough after shot 2 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.04 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = -3.458 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.20.03_batch_tomo_06_11_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 111.9 In SetExposureForMean 120, exposure time changed to 3.2400 Image shift -0.167 0.025 IS units -233.2 430.6 unbinned pixels; need stage -0.141 -0.134 if reset Tilting to -64 -64.00 degrees 10.3 -7.7 unbinned pixels; 3.8 2.5 nm along two shift axes; 2.4 3.9 nm on specimen axes Rate = 22.60968 A/sec 5.8 0.7 unbinned pixels; 0.4 2.0 nm along two shift axes; -0.4 2.0 nm on specimen axes Rate = 10.29546 A/sec Drift is low enough after shot 2 Measured defocus = -4.35 microns changed by 0.10 to target drift = 0.12 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.10 nm/sec Defocus = 0.932 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.21.19_batch_tomo_06_11_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 111.4 In SetExposureForMean 120, exposure time changed to 3.6000 Image shift 0.423 -1.226 IS units 3737.6 15.5 unbinned pixels; need stage -0.120 3.014 if reset Tilting to -66 5.7 -7.7 unbinned pixels; 3.2 1.0 nm along two shift axes; 2.5 2.2 nm on specimen axes Rate = 16.76589 A/sec 5.0 3.0 unbinned pixels; 0.4 2.0 nm along two shift axes; -1.2 1.7 nm on specimen axes Rate = 10.38637 A/sec Drift is low enough after shot 2 Measured defocus = -4.35 microns changed by 0.10 to target drift = 0.11 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.04 nm/sec Defocus = 1.005 um Dewars are NOT busy filling 30 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.22.24_batch_tomo_06_11_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 112.8 In SetExposureForMean 120, exposure time changed to 3.9600 Image shift 0.474 -1.303 IS units 3997.6 -50.3 unbinned pixels; need stage -0.106 3.479 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -15.6 0.8 unbinned pixels; 2.1 5.1 nm along two shift axes; 0.2 -5.5 nm on specimen axes Rate = 27.35986 A/sec -4.2 1.6 unbinned pixels; 1.0 1.2 nm along two shift axes; -0.4 -1.5 nm on specimen axes Rate = 7.88638 A/sec Drift is low enough after shot 2 Measured defocus = -4.09 microns changed by -0.16 to target drift = 0.13 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -3.607 um Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.23.37_batch_tomo_06_11_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 137.1 In SetExposureForMean 120, exposure time changed to 3.4800 Image shift -0.172 0.053 IS units -312.2 417.9 unbinned pixels; need stage -0.134 -0.178 if reset Tilting to 48 Correcting tilt backlash -4.9 -7.7 unbinned pixels; 2.0 2.5 nm along two shift axes; 2.8 -1.5 nm on specimen axes Rate = 16.10745 A/sec 1.0 0.4 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.95564 A/sec Drift is low enough after shot 2 Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.03 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -3.598 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.24.44_batch_tomo_06_11_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 109.5 In SetExposureForMean 120, exposure time changed to 3.8400 Image shift -0.121 -0.061 IS units 46.8 386.3 unbinned pixels; need stage -0.134 0.006 if reset Tilting to -68 -68.00 degrees 21.3 -12.4 unbinned pixels; 6.6 5.6 nm along two shift axes; 3.7 7.8 nm on specimen axes Rate = 43.09486 A/sec 4.5 2.2 unbinned pixels; 0.2 1.8 nm along two shift axes; -0.9 1.5 nm on specimen axes Rate = 8.92027 A/sec Drift is low enough after shot 2 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.10 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.09 nm/sec Defocus = 1.073 um Dewars are NOT busy filling 32 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.25.56_batch_tomo_06_11_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 108.5 In SetExposureForMean 120, exposure time changed to 4.4400 Image shift 0.546 -1.449 IS units 4463.4 -106.2 unbinned pixels; need stage -0.101 4.222 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Using peak at -2.9,-5.2, rejecting 1.34 times higher peak at 0.0,0.5 Measured defocus = 0.07 microns changed by -4.32 to target drift = 0.78 nm/sec Focus area changed from stored state, axis position -2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_07.st Running script 1 at item 13 (-380.49, -436.14) 23:26:58 Refine ZLP: additional offset = -0.8 eV; total offset = -13.9 eV Item 13: Stage: -380.49 -436.14 -223 Label: 13 Note: Sec 12 - anchor_maps.map Target defocus is: -4.25 um Target defocus is: -4.50 um Target defocus = -4.5 Angle = -4.40, increment = 0.60, shift = -0.9 -7.3 pixels, -0.004 um Y Angle = -3.20, increment = 1.80, shift = -10.3 -13.0 pixels, -0.027 um Y Angle = 0.41, increment = 5.41, shift = -21.7 -16.4 pixels, -0.053 um Y Angle = 3.00, increment = 8.00, shift = -16.4 -18.2 pixels, -0.042 um Y Rough eucentricity: changing Z by 0.30 to -223.08, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = 44.1, 12.9 pixels, 0.018, 0.103 um, IS = -0.018, -0.103 Angle = 11.00, shift = 6.2, 6.3 pixels, 0.013, 0.016 um, IS = -0.031, -0.119 Angle = 4.00, shift = 50.5, 14.7 pixels, 0.020, 0.118 um, IS = -0.051, -0.238 Angle = -4.00, shift = 0.5, -17.4 pixels, -0.040, -0.003 um, IS = -0.012, -0.234 Angle = -11.00, shift = 1.5, -3.5 pixels, -0.008, 0.002 um, IS = -0.003, -0.237 Angle = -18.00, shift = 30.9, 10.6 pixels, 0.016, 0.073 um, IS = -0.020, -0.310 Angle = -24.00, shift = 66.9, 14.0 pixels, 0.015, 0.155 um, IS = -0.034, -0.465 General solution delZ -0.46, delY 0.39, yZero -0.21 solution with assumed YZero -0.24: delZ -0.46, delY -0.04 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.46 microns and lateral displacement of -0.39 microns Disparity in stage position after aligning to center of map frame: -0.09 -0.41 Aligning to target with image shift equivalent to stage shift: -0.09 -0.41 Disparity in stage position after aligning to map item itself: -0.09 -0.38 (align shift -0.02 -0.01) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -380.48 -436.11 -222.61 -0.7 -1.0 unbinned pixels; 0.3 0.4 nm along two shift axes; 0.4 -0.2 nm on specimen axes Rate = 2.17814 A/sec Drift is low enough after shot 1 Measured defocus = 1.57 microns changed by -6.07 to target drift = 0.02 nm/sec Measured defocus = -4.60 microns changed by 0.10 to target drift = 0.03 nm/sec Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.03 nm/sec Defocus = -2.082 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.31.51_batch_tomo_07_13_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 132.6 In SetExposureForMean 120, exposure time changed to 1.0800 Image shift -0.257 0.319 IS units -1118.3 395.7 unbinned pixels; need stage -0.102 -0.415 if reset Tilting to -8 Correcting tilt backlash 9.3 -1.6 unbinned pixels; 1.6 2.9 nm along two shift axes; 0.3 3.3 nm on specimen axes Rate = 16.56507 A/sec 8.1 -0.8 unbinned pixels; 1.2 2.6 nm along two shift axes; 0.0 2.9 nm on specimen axes Rate = 14.33458 A/sec 4.0 -0.6 unbinned pixels; 0.7 1.3 nm along two shift axes; 0.1 1.4 nm on specimen axes Rate = 7.1304 A/sec Drift is low enough after shot 3 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.08 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -2.165 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.33.01_batch_tomo_07_13_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 119.4 In SetExposureForMean 120, change by a factor of 1.0049 would require too small a change in exposure time Image shift -0.259 0.280 IS units -1013.8 439.8 unbinned pixels; need stage -0.120 -0.376 if reset Tilting to -12 -12.00 degrees -8.9 -0.2 unbinned pixels; 1.0 3.0 nm along two shift axes; 0.3 -3.1 nm on specimen axes Rate = 15.678 A/sec -3.5 3.8 unbinned pixels; 1.7 0.7 nm along two shift axes; -1.2 -1.3 nm on specimen axes Rate = 8.97224 A/sec Drift is low enough after shot 2 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.08 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -1.974 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.34.04_batch_tomo_07_13_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0059 would require too small a change in exposure time Image shift -0.332 0.414 IS units -1449.1 510.1 unbinned pixels; need stage -0.131 -0.541 if reset Tilting to -14 2.2 0.0 unbinned pixels; 0.2 0.7 nm along two shift axes; -0.1 0.8 nm on specimen axes Rate = 3.78642 A/sec Drift is low enough after shot 1 Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.06 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -1.823 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.35.00_batch_tomo_07_13_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0059 would require too small a change in exposure time Image shift -0.378 0.522 IS units -1786.5 531.3 unbinned pixels; need stage -0.128 -0.669 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 11.4 -7.3 unbinned pixels; 3.8 2.9 nm along two shift axes; 2.2 4.2 nm on specimen axes Rate = 23.72196 A/sec 7.9 0.7 unbinned pixels; 0.7 2.7 nm along two shift axes; -0.5 2.8 nm on specimen axes Rate = 13.962 A/sec Drift is low enough after shot 2 Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.09 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -2.199 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.36.06_batch_tomo_07_13_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0057 would require too small a change in exposure time Image shift -0.222 0.213 IS units -795.3 401.9 unbinned pixels; need stage -0.114 -0.296 if reset Tilting to -4 Correcting tilt backlash -7.0 -1.1 unbinned pixels; 0.4 2.5 nm along two shift axes; 0.6 -2.4 nm on specimen axes Rate = 12.51378 A/sec Drift is low enough after shot 1 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.08 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -2.249 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.37.05_batch_tomo_07_13_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 119.2 In SetExposureForMean 120, change by a factor of 1.0068 would require too small a change in exposure time Image shift -0.203 0.182 IS units -692.6 380.8 unbinned pixels; need stage -0.110 -0.258 if reset Tilting to -16 -16.00 degrees -6.4 -1.3 unbinned pixels; 0.3 2.3 nm along two shift axes; 0.6 -2.2 nm on specimen axes Rate = 11.52352 A/sec Drift is low enough after shot 1 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.08 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -1.723 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.38.04_batch_tomo_07_13_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 119.0 In SetExposureForMean 120, change by a factor of 1.0090 would require too small a change in exposure time Image shift -0.373 0.494 IS units -1704.5 545.5 unbinned pixels; need stage -0.135 -0.645 if reset Tilting to -18 6.7 -7.1 unbinned pixels; 3.1 1.4 nm along two shift axes; 2.3 2.6 nm on specimen axes Rate = 17.15208 A/sec 5.9 0.8 unbinned pixels; 0.4 2.0 nm along two shift axes; -0.5 2.0 nm on specimen axes Rate = 10.44262 A/sec Drift is low enough after shot 2 Measured defocus = -4.60 microns changed by 0.10 to target drift = 0.08 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.07 nm/sec Defocus = -1.651 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.39.11_batch_tomo_07_13_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 118.5 In SetExposureForMean 120, change by a factor of 1.0125 would require too small a change in exposure time Image shift -0.342 0.458 IS units -1578.9 495.5 unbinned pixels; need stage -0.122 -0.604 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 12.6 -0.4 unbinned pixels; 1.6 4.1 nm along two shift axes; -0.2 4.4 nm on specimen axes Rate = 22.15648 A/sec 6.0 -0.8 unbinned pixels; 1.0 1.9 nm along two shift axes; 0.1 2.1 nm on specimen axes Rate = 10.58426 A/sec Drift is low enough after shot 2 Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.08 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -2.252 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.40.17_batch_tomo_07_13_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 118.8 In SetExposureForMean 120, change by a factor of 1.0103 would require too small a change in exposure time Image shift -0.201 0.189 IS units -709.3 367.0 unbinned pixels; need stage -0.104 -0.263 if reset Tilting to 0 Correcting tilt backlash -9.7 0.0 unbinned pixels; 1.1 3.2 nm along two shift axes; 0.3 -3.4 nm on specimen axes Rate = 16.98247 A/sec -6.7 0.6 unbinned pixels; 1.0 2.2 nm along two shift axes; -0.0 -2.4 nm on specimen axes Rate = 11.85994 A/sec Drift is low enough after shot 2 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.15 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.14 nm/sec Defocus = -2.335 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.41.22_batch_tomo_07_13_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 118.6 In SetExposureForMean 120, change by a factor of 1.0116 would require too small a change in exposure time Image shift -0.194 0.181 IS units -681.7 355.5 unbinned pixels; need stage -0.101 -0.253 if reset Tilting to -20 -20.00 degrees -1.1 -4.8 unbinned pixels; 1.5 0.9 nm along two shift axes; 1.7 -0.2 nm on specimen axes Rate = 8.79021 A/sec Drift is low enough after shot 1 Measured defocus = -4.60 microns changed by 0.10 to target drift = 0.01 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.03 nm/sec Defocus = -1.573 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.42.21_batch_tomo_07_13_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 118.2 In SetExposureForMean 120, change by a factor of 1.0153 would require too small a change in exposure time Image shift -0.321 0.405 IS units -1413.5 488.8 unbinned pixels; need stage -0.125 -0.549 if reset Tilting to -22 7.0 -6.6 unbinned pixels; 3.0 1.5 nm along two shift axes; 2.1 2.7 nm on specimen axes Rate = 16.89581 A/sec 5.4 1.6 unbinned pixels; 0.1 2.0 nm along two shift axes; -0.7 1.8 nm on specimen axes Rate = 9.89532 A/sec Drift is low enough after shot 2 Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.10 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -1.451 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.43.23_batch_tomo_07_13_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 117.8 In SetExposureForMean 120, change by a factor of 1.0193 would require too small a change in exposure time Image shift -0.288 0.354 IS units -1244.5 448.1 unbinned pixels; need stage -0.116 -0.490 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 10.1 -0.1 unbinned pixels; 1.2 3.3 nm along two shift axes; -0.3 3.5 nm on specimen axes Rate = 17.71803 A/sec 5.9 -0.2 unbinned pixels; 0.7 1.9 nm along two shift axes; -0.1 2.1 nm on specimen axes Rate = 10.26925 A/sec Drift is low enough after shot 2 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.01 nm/sec Defocus = -2.361 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.44.30_batch_tomo_07_13_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 118.4 In SetExposureForMean 120, change by a factor of 1.0139 would require too small a change in exposure time Image shift -0.190 0.177 IS units -667.5 347.2 unbinned pixels; need stage -0.099 -0.247 if reset Tilting to 4 Correcting tilt backlash -16.7 4.5 unbinned pixels; 3.4 5.0 nm along two shift axes; -1.1 -6.0 nm on specimen axes Rate = 30.36538 A/sec -13.1 3.7 unbinned pixels; 2.7 3.9 nm along two shift axes; -0.9 -4.7 nm on specimen axes Rate = 23.85502 A/sec -9.2 2.8 unbinned pixels; 2.0 2.7 nm along two shift axes; -0.7 -3.3 nm on specimen axes Rate = 16.91502 A/sec -7.9 2.1 unbinned pixels; 1.6 2.4 nm along two shift axes; -0.5 -2.8 nm on specimen axes Rate = 14.26384 A/sec -6.5 2.0 unbinned pixels; 1.4 1.9 nm along two shift axes; -0.5 -2.3 nm on specimen axes Rate = 11.90075 A/sec Drift is low enough after shot 5 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.17 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.13 nm/sec Defocus = -2.428 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.45.56_batch_tomo_07_13_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 117.9 In SetExposureForMean 120, change by a factor of 1.0176 would require too small a change in exposure time Image shift -0.195 0.186 IS units -696.8 352.6 unbinned pixels; need stage -0.100 -0.258 if reset Tilting to -24 -24.00 degrees -1.4 -5.5 unbinned pixels; 1.7 1.1 nm along two shift axes; 2.0 -0.3 nm on specimen axes Rate = 10.12414 A/sec Drift is low enough after shot 1 Measured defocus = -4.61 microns changed by 0.11 to target drift = 0.04 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = -1.321 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.46.55_batch_tomo_07_13_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 117.2 In SetExposureForMean 120, change by a factor of 1.0245 would require too small a change in exposure time Image shift -0.266 0.291 IS units -1049.6 449.0 unbinned pixels; need stage -0.122 -0.422 if reset Tilting to -26 3.5 -7.4 unbinned pixels; 2.9 0.3 nm along two shift axes; 2.5 1.5 nm on specimen axes Rate = 14.34151 A/sec 1.9 2.2 unbinned pixels; 0.5 0.9 nm along two shift axes; -0.8 0.6 nm on specimen axes Rate = 5.02962 A/sec Drift is low enough after shot 2 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.07 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.249 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.47.57_batch_tomo_07_13_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 116.6 In SetExposureForMean 120, change by a factor of 1.0292 would require too small a change in exposure time Image shift -0.218 0.192 IS units -734.7 409.5 unbinned pixels; need stage -0.118 -0.304 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 4.5 1.4 unbinned pixels; 0.1 1.7 nm along two shift axes; -0.6 1.5 nm on specimen axes Rate = 8.31396 A/sec Drift is low enough after shot 1 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -2.475 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.48.59_batch_tomo_07_13_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0225 would require too small a change in exposure time Image shift -0.201 0.180 IS units -686.9 375.7 unbinned pixels; need stage -0.108 -0.256 if reset Tilting to 8 Correcting tilt backlash -21.2 6.8 unbinned pixels; 4.7 6.2 nm along two shift axes; -1.7 -7.6 nm on specimen axes Rate = 38.96651 A/sec -9.5 4.9 unbinned pixels; 2.7 2.6 nm along two shift axes; -1.4 -3.5 nm on specimen axes Rate = 18.79174 A/sec -7.4 2.3 unbinned pixels; 1.6 2.2 nm along two shift axes; -0.6 -2.7 nm on specimen axes Rate = 13.56473 A/sec Drift is low enough after shot 3 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.18 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.13 nm/sec Defocus = -2.570 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.50.09_batch_tomo_07_13_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 116.9 In SetExposureForMean 120, change by a factor of 1.0261 would require too small a change in exposure time Image shift -0.214 0.214 IS units -790.1 379.0 unbinned pixels; need stage -0.106 -0.295 if reset Tilting to -28 -28.00 degrees 0.2 -0.4 unbinned pixels; 0.2 0.0 nm along two shift axes; 0.1 0.1 nm on specimen axes Rate = 0.79014 A/sec Drift is low enough after shot 1 Measured defocus = -4.62 microns changed by 0.12 to target drift = 0.06 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -1.126 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.51.09_batch_tomo_07_13_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, change by a factor of 1.0323 would require too small a change in exposure time Image shift -0.214 0.152 IS units -623.0 436.7 unbinned pixels; need stage -0.131 -0.266 if reset Tilting to -30 2.4 -6.4 unbinned pixels; 2.4 0.0 nm along two shift axes; 2.2 1.0 nm on specimen axes Rate = 12.00247 A/sec Drift is low enough after shot 1 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.07 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.07 nm/sec Defocus = -1.059 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.52.06_batch_tomo_07_13_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 115.3 In SetExposureForMean 120, change by a factor of 1.0410 would require too small a change in exposure time Image shift -0.164 0.058 IS units -319.2 390.3 unbinned pixels; need stage -0.124 -0.145 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees 3.6 3.4 unbinned pixels; 0.7 1.6 nm along two shift axes; -1.3 1.1 nm on specimen axes Rate = 8.64432 A/sec Drift is low enough after shot 1 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.639 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.53.08_batch_tomo_07_13_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, change by a factor of 1.0317 would require too small a change in exposure time Image shift -0.216 0.184 IS units -710.0 412.0 unbinned pixels; need stage -0.120 -0.268 if reset Tilting to 12 Correcting tilt backlash -13.4 -0.1 unbinned pixels; 1.5 4.4 nm along two shift axes; 0.4 -4.7 nm on specimen axes Rate = 23.52091 A/sec -6.1 4.7 unbinned pixels; 2.3 1.5 nm along two shift axes; -1.5 -2.3 nm on specimen axes Rate = 13.53654 A/sec Drift is low enough after shot 2 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.21 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.13 nm/sec Defocus = -2.735 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.54.12_batch_tomo_07_13_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 115.5 In SetExposureForMean 120, change by a factor of 1.0386 would require too small a change in exposure time Image shift -0.196 0.156 IS units -614.1 384.8 unbinned pixels; need stage -0.114 -0.235 if reset Tilting to -32 -32.00 degrees 2.3 -5.6 unbinned pixels; 2.1 0.1 nm along two shift axes; 1.9 1.0 nm on specimen axes Rate = 10.71851 A/sec Drift is low enough after shot 1 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.06 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -0.972 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.55.13_batch_tomo_07_13_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 114.5 In SetExposureForMean 120, change by a factor of 1.0478 would require too small a change in exposure time Image shift -0.133 -0.044 IS units -12.6 402.5 unbinned pixels; need stage -0.138 -0.021 if reset Tilting to -34 5.6 -4.5 unbinned pixels; 2.1 1.3 nm along two shift axes; 1.4 2.1 nm on specimen axes Rate = 12.53421 A/sec Drift is low enough after shot 1 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.12 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.11 nm/sec Defocus = -0.955 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.56.10_batch_tomo_07_13_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 113.4 In SetExposureForMean 120, exposure time changed to 1.2000 WARNING: Desired exposure time (1.143) differs from actual one (1.200) by more than 5% Image shift -0.105 -0.095 IS units 154.0 375.6 unbinned pixels; need stage -0.134 0.051 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -6.8 1.6 unbinned pixels; 1.3 2.0 nm along two shift axes; -0.4 -2.4 nm on specimen axes Rate = 12.1725 A/sec Drift is low enough after shot 1 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.18 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.14 nm/sec Defocus = -2.799 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.57.12_batch_tomo_07_13_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 127.4 In SetExposureForMean 120, exposure time changed to 1.0800 Image shift -0.200 0.139 IS units -573.6 411.4 unbinned pixels; need stage -0.124 -0.223 if reset Tilting to 16 Correcting tilt backlash -6.3 -1.9 unbinned pixels; 0.1 2.3 nm along two shift axes; 0.9 -2.1 nm on specimen axes Rate = 11.58898 A/sec Drift is low enough after shot 1 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.10 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.04 nm/sec Defocus = -2.862 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.58.11_batch_tomo_07_13_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 113.5 In SetExposureForMean 120, exposure time changed to 1.2000 WARNING: Desired exposure time (1.142) differs from actual one (1.200) by more than 5% Image shift -0.168 0.069 IS units -351.0 391.5 unbinned pixels; need stage -0.124 -0.142 if reset Tilting to -36 -36.00 degrees -0.6 -1.9 unbinned pixels; 0.6 0.4 nm along two shift axes; 0.7 -0.1 nm on specimen axes Rate = 3.45468 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.08 nm/sec Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.04 nm/sec Defocus = -0.860 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-12_23.59.12_batch_tomo_07_13_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 124.5 In SetExposureForMean 120, change by a factor of 0.9641 would require too small a change in exposure time Image shift -0.088 -0.160 IS units 346.3 392.6 unbinned pixels; need stage -0.146 0.136 if reset Tilting to -38 5.7 -5.4 unbinned pixels; 2.5 1.2 nm along two shift axes; 1.7 2.2 nm on specimen axes Rate = 13.78567 A/sec Drift is low enough after shot 1 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.18 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -0.836 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.00.09_batch_tomo_07_13_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 123.2 In SetExposureForMean 120, change by a factor of 0.9741 would require too small a change in exposure time Image shift -0.056 -0.212 IS units 519.7 354.7 unbinned pixels; need stage -0.138 0.219 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -16.0 2.1 unbinned pixels; 2.6 5.1 nm along two shift axes; -0.2 -5.7 nm on specimen axes Rate = 28.3765 A/sec -8.9 1.7 unbinned pixels; 1.6 2.7 nm along two shift axes; -0.3 -3.2 nm on specimen axes Rate = 15.93335 A/sec -9.5 0.6 unbinned pixels; 1.3 3.1 nm along two shift axes; 0.1 -3.4 nm on specimen axes Rate = 16.70392 A/sec -7.7 0.4 unbinned pixels; 1.0 2.5 nm along two shift axes; 0.1 -2.7 nm on specimen axes Rate = 13.53018 A/sec Drift is low enough after shot 4 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.22 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.13 nm/sec Defocus = -2.941 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.01.29_batch_tomo_07_13_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 125.1 In SetExposureForMean 120, change by a factor of 0.9594 would require too small a change in exposure time Image shift -0.193 0.117 IS units -505.8 414.7 unbinned pixels; need stage -0.127 -0.202 if reset Tilting to 20 Correcting tilt backlash -3.2 -3.2 unbinned pixels; 0.7 1.4 nm along two shift axes; 1.2 -1.0 nm on specimen axes Rate = 7.95165 A/sec Drift is low enough after shot 1 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.08 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.11 nm/sec Defocus = -3.018 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.02.28_batch_tomo_07_13_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 123.6 In SetExposureForMean 120, change by a factor of 0.9709 would require too small a change in exposure time Image shift -0.143 0.002 IS units -144.9 386.7 unbinned pixels; need stage -0.129 -0.068 if reset Tilting to -40 -40.00 degrees 5.0 -5.5 unbinned pixels; 2.4 1.0 nm along two shift axes; 1.8 1.9 nm on specimen axes Rate = 13.054 A/sec Drift is low enough after shot 1 Measured defocus = -4.61 microns changed by 0.11 to target drift = 0.13 nm/sec Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.04 nm/sec Defocus = -0.678 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.03.29_batch_tomo_07_13_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 121.8 In SetExposureForMean 120, change by a factor of 0.9858 would require too small a change in exposure time Image shift -0.032 -0.285 IS units 742.1 358.3 unbinned pixels; need stage -0.146 0.327 if reset Tilting to -42 5.4 -1.4 unbinned pixels; 1.1 1.6 nm along two shift axes; 0.3 1.9 nm on specimen axes Rate = 9.82697 A/sec Drift is low enough after shot 1 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.10 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -0.693 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.04.26_batch_tomo_07_13_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 120.2 In SetExposureForMean 120, change by a factor of 0.9987 would require too small a change in exposure time Image shift -0.012 -0.308 IS units 823.8 325.4 unbinned pixels; need stage -0.137 0.378 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -16.6 1.9 unbinned pixels; 2.6 5.3 nm along two shift axes; -0.2 -5.9 nm on specimen axes Rate = 29.26978 A/sec -9.0 1.6 unbinned pixels; 1.6 2.8 nm along two shift axes; -0.3 -3.2 nm on specimen axes Rate = 16.09906 A/sec -5.6 1.4 unbinned pixels; 1.1 1.7 nm along two shift axes; -0.3 -2.0 nm on specimen axes Rate = 10.17756 A/sec Drift is low enough after shot 3 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.17 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.11 nm/sec Defocus = -3.092 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.05.41_batch_tomo_07_13_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 122.1 In SetExposureForMean 120, change by a factor of 0.9829 would require too small a change in exposure time Image shift -0.165 0.061 IS units -326.9 392.1 unbinned pixels; need stage -0.125 -0.138 if reset Tilting to 24 Correcting tilt backlash 0.2 -3.0 unbinned pixels; 1.0 0.3 nm along two shift axes; 1.0 0.2 nm on specimen axes Rate = 5.26258 A/sec Drift is low enough after shot 1 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.06 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.05 nm/sec Defocus = -3.176 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.06.40_batch_tomo_07_13_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 120.5 In SetExposureForMean 120, change by a factor of 0.9965 would require too small a change in exposure time Image shift -0.122 -0.071 IS units 73.3 398.9 unbinned pixels; need stage -0.140 0.014 if reset Tilting to -44 -44.00 degrees 4.6 -5.3 unbinned pixels; 2.3 0.9 nm along two shift axes; 1.7 1.8 nm on specimen axes Rate = 12.25278 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -0.612 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.07.41_batch_tomo_07_13_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 118.7 In SetExposureForMean 120, change by a factor of 1.0113 would require too small a change in exposure time Image shift 0.026 -0.400 IS units 1109.0 311.0 unbinned pixels; need stage -0.141 0.531 if reset Tilting to -46 1.0 -3.2 unbinned pixels; 1.2 0.1 nm along two shift axes; 1.1 0.4 nm on specimen axes Rate = 5.92919 A/sec Drift is low enough after shot 1 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.05 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -0.531 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.08.38_batch_tomo_07_13_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 116.4 In SetExposureForMean 120, change by a factor of 1.0305 would require too small a change in exposure time Image shift 0.050 -0.438 IS units 1237.1 281.4 unbinned pixels; need stage -0.135 0.617 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -15.9 1.9 unbinned pixels; 2.5 5.1 nm along two shift axes; -0.2 -5.6 nm on specimen axes Rate = 28.1326 A/sec -7.4 2.4 unbinned pixels; 1.7 2.1 nm along two shift axes; -0.6 -2.7 nm on specimen axes Rate = 13.58022 A/sec Drift is low enough after shot 2 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.20 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.12 nm/sec Defocus = -3.249 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.09.47_batch_tomo_07_13_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 118.8 In SetExposureForMean 120, change by a factor of 1.0104 would require too small a change in exposure time Image shift -0.124 -0.033 IS units -33.0 367.8 unbinned pixels; need stage -0.126 -0.026 if reset Tilting to 28 Correcting tilt backlash -5.2 -2.6 unbinned pixels; 0.2 2.0 nm along two shift axes; 1.1 -1.7 nm on specimen axes Rate = 10.18385 A/sec Drift is low enough after shot 1 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = -3.315 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.10.46_batch_tomo_07_13_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 117.2 In SetExposureForMean 120, change by a factor of 1.0242 would require too small a change in exposure time Image shift -0.087 -0.158 IS units 341.6 387.1 unbinned pixels; need stage -0.144 0.123 if reset Tilting to -48 -48.00 degrees 1.7 -3.4 unbinned pixels; 1.3 0.1 nm along two shift axes; 1.1 0.7 nm on specimen axes Rate = 6.70945 A/sec Drift is low enough after shot 1 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.08 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -0.444 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.11.49_batch_tomo_07_13_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, change by a factor of 1.0484 would require too small a change in exposure time Image shift 0.068 -0.487 IS units 1386.3 279.0 unbinned pixels; need stage -0.139 0.719 if reset Tilting to -50 4.0 -5.0 unbinned pixels; 2.1 0.7 nm along two shift axes; 1.6 1.6 nm on specimen axes Rate = 11.23074 A/sec Drift is low enough after shot 1 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.09 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.14 nm/sec Defocus = -0.386 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.12.46_batch_tomo_07_13_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 112.3 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift 0.089 -0.525 IS units 1508.6 257.6 unbinned pixels; need stage -0.135 0.817 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -14.4 2.3 unbinned pixels; 2.4 4.5 nm along two shift axes; -0.4 -5.1 nm on specimen axes Rate = 25.51785 A/sec -7.1 4.7 unbinned pixels; 2.4 1.8 nm along two shift axes; -1.4 -2.6 nm on specimen axes Rate = 14.95392 A/sec -4.8 3.1 unbinned pixels; 1.6 1.2 nm along two shift axes; -0.9 -1.8 nm on specimen axes Rate = 10.02245 A/sec Drift is low enough after shot 3 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.21 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.23 nm/sec Defocus = -3.417 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.14.01_batch_tomo_07_13_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 126.5 In SetExposureForMean 120, exposure time changed to 1.2000 Image shift -0.094 -0.108 IS units 200.0 357.4 unbinned pixels; need stage -0.129 0.068 if reset Tilting to 32 Correcting tilt backlash -1.6 -7.1 unbinned pixels; 2.2 1.4 nm along two shift axes; 2.5 -0.4 nm on specimen axes Rate = 12.91727 A/sec Drift is low enough after shot 1 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.09 nm/sec Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.15 nm/sec Defocus = -3.431 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.15.00_batch_tomo_07_13_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 112.7 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift -0.056 -0.221 IS units 542.8 363.5 unbinned pixels; need stage -0.142 0.212 if reset Tilting to -52 -52.00 degrees 8.2 -5.8 unbinned pixels; 2.9 2.0 nm along two shift axes; 1.8 3.1 nm on specimen axes Rate = 17.59828 A/sec 5.7 2.6 unbinned pixels; 0.2 2.2 nm along two shift axes; -1.1 1.9 nm on specimen axes Rate = 11.05774 A/sec Drift is low enough after shot 2 Measured defocus = -4.64 microns changed by 0.14 to target drift = 0.20 nm/sec Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.21 nm/sec Defocus = -0.288 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.16.08_batch_tomo_07_13_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 120.5 In SetExposureForMean 120, change by a factor of 0.9931 would require too small a change in exposure time Image shift 0.135 -0.626 IS units 1829.5 227.1 unbinned pixels; need stage -0.134 1.039 if reset Tilting to -54 5.2 -6.0 unbinned pixels; 2.6 1.0 nm along two shift axes; 1.9 2.0 nm on specimen axes Rate = 13.95487 A/sec Drift is low enough after shot 1 Measured defocus = -4.61 microns changed by 0.11 to target drift = 0.06 nm/sec Measured defocus = -4.31 microns changed by -0.19 to target drift = 0.10 nm/sec Defocus = -0.368 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.17.05_batch_tomo_07_13_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0182 would require too small a change in exposure time Image shift 0.185 -0.737 IS units 2179.7 196.9 unbinned pixels; need stage -0.135 1.300 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -13.8 2.9 unbinned pixels; 2.6 4.2 nm along two shift axes; -0.6 -4.9 nm on specimen axes Rate = 24.78717 A/sec -11.8 1.3 unbinned pixels; 1.8 3.8 nm along two shift axes; -0.1 -4.2 nm on specimen axes Rate = 20.88324 A/sec -7.8 1.0 unbinned pixels; 1.2 2.5 nm along two shift axes; -0.1 -2.8 nm on specimen axes Rate = 13.74017 A/sec Drift is low enough after shot 3 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.18 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.21 nm/sec Defocus = -3.548 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.18.21_batch_tomo_07_13_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 121.5 In SetExposureForMean 120, change by a factor of 0.9864 would require too small a change in exposure time Image shift -0.061 -0.195 IS units 469.0 353.5 unbinned pixels; need stage -0.136 0.186 if reset Tilting to 36 Correcting tilt backlash -4.1 -8.5 unbinned pixels; 2.4 2.4 nm along two shift axes; 3.1 -1.2 nm on specimen axes Rate = 16.77358 A/sec -1.8 0.1 unbinned pixels; 0.2 0.6 nm along two shift axes; 0.0 -0.6 nm on specimen axes Rate = 3.07944 A/sec Drift is low enough after shot 2 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.20 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.20 nm/sec Defocus = -3.541 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.19.26_batch_tomo_07_13_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 118.6 In SetExposureForMean 120, change by a factor of 1.0096 would require too small a change in exposure time Image shift -0.008 -0.312 IS units 836.2 318.7 unbinned pixels; need stage -0.135 0.353 if reset Tilting to -56 -56.00 degrees 11.2 -6.7 unbinned pixels; 3.5 2.9 nm along two shift axes; 2.0 4.1 nm on specimen axes Rate = 22.88053 A/sec 5.8 1.3 unbinned pixels; 0.2 2.1 nm along two shift axes; -0.6 2.0 nm on specimen axes Rate = 10.42164 A/sec Drift is low enough after shot 2 Measured defocus = -4.75 microns changed by 0.25 to target drift = 0.18 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.18 nm/sec Defocus = -0.132 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.20.34_batch_tomo_07_13_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 114.2 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.234 -0.842 IS units 2511.5 163.6 unbinned pixels; need stage -0.134 1.579 if reset Tilting to -58 2.9 -4.3 unbinned pixels; 1.8 0.4 nm along two shift axes; 1.4 1.1 nm on specimen axes Rate = 9.1238 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.20 nm/sec Defocus = -0.061 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.21.32_batch_tomo_07_13_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 120.6 In SetExposureForMean 120, change by a factor of 0.9903 would require too small a change in exposure time Image shift 0.267 -0.920 IS units 2756.5 149.2 unbinned pixels; need stage -0.136 1.830 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -17.2 4.9 unbinned pixels; 3.7 5.1 nm along two shift axes; -1.2 -6.2 nm on specimen axes Rate = 31.38481 A/sec -10.8 2.4 unbinned pixels; 2.1 3.3 nm along two shift axes; -0.5 -3.9 nm on specimen axes Rate = 19.39921 A/sec -14.5 0.7 unbinned pixels; 1.9 4.7 nm along two shift axes; 0.2 -5.1 nm on specimen axes Rate = 25.55179 A/sec -9.1 0.3 unbinned pixels; 1.1 3.0 nm along two shift axes; 0.2 -3.2 nm on specimen axes Rate = 15.88806 A/sec -6.0 0.2 unbinned pixels; 0.8 2.0 nm along two shift axes; 0.1 -2.1 nm on specimen axes Rate = 10.52693 A/sec Drift is low enough after shot 5 Measured defocus = -4.27 microns changed by -0.23 to target drift = 0.23 nm/sec Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.10 nm/sec Defocus = -3.650 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.23.04_batch_tomo_07_13_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 125.8 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift -0.008 -0.309 IS units 830.2 317.1 unbinned pixels; need stage -0.135 0.359 if reset Tilting to 40 Correcting tilt backlash -4.6 -8.9 unbinned pixels; 2.4 2.6 nm along two shift axes; 3.2 -1.4 nm on specimen axes Rate = 17.68597 A/sec -2.2 -1.2 unbinned pixels; 0.1 0.9 nm along two shift axes; 0.5 -0.7 nm on specimen axes Rate = 4.43767 A/sec Drift is low enough after shot 2 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.13 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.19 nm/sec Defocus = -3.599 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.24.08_batch_tomo_07_13_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 112.2 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.037 -0.424 IS units 1185.9 304.1 unbinned pixels; need stage -0.141 0.534 if reset Tilting to -60 -60.00 degrees 4.3 -6.6 unbinned pixels; 2.7 0.6 nm along two shift axes; 2.2 1.7 nm on specimen axes Rate = 13.91137 A/sec Drift is low enough after shot 1 Measured defocus = -4.69 microns changed by 0.19 to target drift = 0.09 nm/sec Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.11 nm/sec Defocus = 0.085 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.25.11_batch_tomo_07_13_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, change by a factor of 1.0312 would require too small a change in exposure time Image shift 0.298 -0.984 IS units 2959.7 123.9 unbinned pixels; need stage -0.134 2.085 if reset Tilting to -62 5.7 -6.2 unbinned pixels; 2.7 1.2 nm along two shift axes; 2.0 2.2 nm on specimen axes Rate = 14.78894 A/sec 3.2 3.0 unbinned pixels; 0.6 1.4 nm along two shift axes; -1.1 1.0 nm on specimen axes Rate = 7.70493 A/sec Drift is low enough after shot 2 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.20 nm/sec Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.17 nm/sec Defocus = 0.176 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.26.14_batch_tomo_07_13_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 111.6 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.349 -1.078 IS units 3263.2 76.6 unbinned pixels; need stage -0.127 2.452 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -16.5 2.9 unbinned pixels; 2.9 5.1 nm along two shift axes; -0.5 -5.9 nm on specimen axes Rate = 29.43994 A/sec -15.5 6.3 unbinned pixels; 3.9 4.4 nm along two shift axes; -1.7 -5.6 nm on specimen axes Rate = 29.32075 A/sec -12.1 3.0 unbinned pixels; 2.4 3.6 nm along two shift axes; -0.7 -4.3 nm on specimen axes Rate = 21.89359 A/sec -9.9 2.4 unbinned pixels; 2.0 3.0 nm along two shift axes; -0.5 -3.6 nm on specimen axes Rate = 17.92469 A/sec -11.0 1.9 unbinned pixels; 1.9 3.4 nm along two shift axes; -0.3 -3.9 nm on specimen axes Rate = 19.66208 A/sec -8.6 1.8 unbinned pixels; 1.6 2.6 nm along two shift axes; -0.4 -3.1 nm on specimen axes Rate = 15.35565 A/sec -6.4 1.5 unbinned pixels; 1.2 1.9 nm along two shift axes; -0.3 -2.3 nm on specimen axes Rate = 11.4975 A/sec Drift is low enough after shot 7 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.26 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.25 nm/sec Defocus = -3.645 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.27.53_batch_tomo_07_13_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 127.4 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.047 -0.433 IS units 1219.3 284.1 unbinned pixels; need stage -0.135 0.568 if reset Tilting to 44 Correcting tilt backlash -10.6 -1.5 unbinned pixels; 0.7 3.7 nm along two shift axes; 0.9 -3.7 nm on specimen axes Rate = 18.78388 A/sec -7.9 4.2 unbinned pixels; 2.3 2.1 nm along two shift axes; -1.2 -2.9 nm on specimen axes Rate = 15.61874 A/sec -3.9 1.4 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.4 -1.4 nm on specimen axes Rate = 7.25193 A/sec Drift is low enough after shot 3 Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.20 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.19 nm/sec Defocus = -3.578 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.29.03_batch_tomo_07_13_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 113.0 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.081 -0.527 IS units 1506.9 279.4 unbinned pixels; need stage -0.143 0.728 if reset Tilting to -64 -64.00 degrees 4.5 -5.7 unbinned pixels; 2.4 0.8 nm along two shift axes; 1.9 1.8 nm on specimen axes Rate = 12.7771 A/sec Drift is low enough after shot 1 Measured defocus = -4.64 microns changed by 0.14 to target drift = 0.13 nm/sec Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.21 nm/sec Defocus = 0.189 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.30.07_batch_tomo_07_13_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 115.2 In SetExposureForMean 120, change by a factor of 1.0340 would require too small a change in exposure time Image shift 0.364 -1.127 IS units 3411.9 80.9 unbinned pixels; need stage -0.133 2.746 if reset Tilting to -66 1.4 -3.8 unbinned pixels; 1.4 0.0 nm along two shift axes; 1.3 0.6 nm on specimen axes Rate = 7.20862 A/sec Drift is low enough after shot 1 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.26 nm/sec Measured defocus = -4.55 microns changed by 0.05 to target drift = 0.11 nm/sec Defocus = 0.314 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.31.10_batch_tomo_07_13_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 109.8 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.409 -1.202 IS units 3660.8 30.3 unbinned pixels; need stage -0.123 3.180 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -18.7 0.9 unbinned pixels; 2.5 6.1 nm along two shift axes; 0.3 -6.6 nm on specimen axes Rate = 32.89731 A/sec -13.3 7.5 unbinned pixels; 4.0 3.5 nm along two shift axes; -2.2 -4.9 nm on specimen axes Rate = 26.73137 A/sec -11.3 4.1 unbinned pixels; 2.7 3.3 nm along two shift axes; -1.1 -4.1 nm on specimen axes Rate = 21.05923 A/sec -9.2 3.1 unbinned pixels; 2.1 2.7 nm along two shift axes; -0.8 -3.3 nm on specimen axes Rate = 17.0388 A/sec -8.1 2.5 unbinned pixels; 1.8 2.4 nm along two shift axes; -0.6 -2.9 nm on specimen axes Rate = 14.89663 A/sec -7.4 2.2 unbinned pixels; 1.6 2.2 nm along two shift axes; -0.5 -2.6 nm on specimen axes Rate = 13.43764 A/sec Drift is low enough after shot 6 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.31 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.24 nm/sec Defocus = -3.626 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.32.45_batch_tomo_07_13_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 126.1 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.102 -0.544 IS units 1575.4 239.7 unbinned pixels; need stage -0.131 0.791 if reset Tilting to 48 Correcting tilt backlash -11.2 -4.1 unbinned pixels; 0.1 4.2 nm along two shift axes; 1.8 -3.8 nm on specimen axes Rate = 21.02978 A/sec -2.7 4.4 unbinned pixels; 1.8 0.4 nm along two shift axes; -1.5 -1.1 nm on specimen axes Rate = 9.13708 A/sec Drift is low enough after shot 2 Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.19 nm/sec Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.12 nm/sec Defocus = -3.746 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.33.50_batch_tomo_07_13_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 111.5 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.144 -0.610 IS units 1793.1 188.8 unbinned pixels; need stage -0.120 0.938 if reset Tilting to -68 -68.00 degrees 45.3 -11.1 unbinned pixels; 9.0 13.7 nm along two shift axes; 2.5 16.2 nm on specimen axes Rate = 81.68798 A/sec 4.6 1.7 unbinned pixels; 0.0 1.7 nm along two shift axes; -0.7 1.5 nm on specimen axes Rate = 8.60187 A/sec Drift is low enough after shot 2 Measured defocus = -4.65 microns changed by 0.15 to target drift = 0.09 nm/sec Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.12 nm/sec Defocus = 0.375 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.35.00_batch_tomo_07_13_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 111.1 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.459 -1.292 IS units 3952.1 -21.4 unbinned pixels; need stage -0.114 3.732 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 0.45 microns changed by -4.95 to target drift = 0.82 nm/sec Focus area changed from stored state, axis position -2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_08.st Running script 1 at item 15 (-383.02, -436.84) 00:36:02 Refine ZLP: additional offset = 0.3 eV; total offset = -13.6 eV Item 15: Stage: -383.02 -436.84 -223 Label: 15 Note: Sec 14 - anchor_maps.map Target defocus is: -4.50 um Target defocus is: -3.50 um Target defocus = -3.5 Angle = -4.40, increment = 0.60, shift = 6.8 -3.4 pixels, 0.014 um Y Angle = -3.20, increment = 1.80, shift = 2.0 -7.5 pixels, 0.003 um Y Angle = 0.41, increment = 5.41, shift = -17.6 -14.9 pixels, -0.044 um Y Angle = 3.00, increment = 8.00, shift = 8.8 -15.5 pixels, 0.016 um Y Rough eucentricity: changing Z by -0.11 to -223.49, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = 37.0, 14.8 pixels, 0.024, 0.088 um, IS = -0.024, -0.088 Angle = 11.00, shift = 44.0, 8.2 pixels, 0.007, 0.102 um, IS = -0.032, -0.190 Angle = 4.00, shift = 28.6, 9.1 pixels, 0.013, 0.067 um, IS = -0.045, -0.257 Angle = -4.00, shift = 1.0, -21.8 pixels, -0.050, -0.003 um, IS = 0.005, -0.254 Angle = -11.00, shift = 49.0, 3.7 pixels, -0.004, 0.112 um, IS = 0.009, -0.366 Angle = -18.00, shift = 74.2, 16.1 pixels, 0.018, 0.172 um, IS = -0.009, -0.538 Angle = -24.00, shift = 76.0, 14.5 pixels, 0.014, 0.176 um, IS = -0.022, -0.714 General solution delZ -0.77, delY 1.45, yZero -0.25 solution with assumed YZero -0.26: delZ -0.77, delY 1.43 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.77 microns and lateral displacement of -1.45 microns Disparity in stage position after aligning to center of map frame: -0.05 -0.40 Aligning to target with image shift equivalent to stage shift: -0.05 -0.40 Disparity in stage position after aligning to map item itself: -0.06 -0.37 (align shift -0.02 -0.01) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -383.01 -436.80 -222.71 0.7 -0.4 unbinned pixels; 0.2 0.2 nm along two shift axes; 0.1 0.3 nm on specimen axes Rate = 1.42539 A/sec Drift is low enough after shot 1 Measured defocus = 1.33 microns changed by -4.83 to target drift = 0.07 nm/sec Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.04 nm/sec Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.06 nm/sec Defocus = -0.999 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.40.56_batch_tomo_08_15_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 131.2 In SetExposureForMean 120, exposure time changed to 1.0800 Image shift -0.223 0.325 IS units -1101.7 298.2 unbinned pixels; need stage -0.069 -0.405 if reset Tilting to -8 Correcting tilt backlash 9.1 -0.1 unbinned pixels; 1.1 3.0 nm along two shift axes; -0.2 3.2 nm on specimen axes Rate = 15.92866 A/sec 6.8 2.0 unbinned pixels; 0.1 2.5 nm along two shift axes; -0.9 2.3 nm on specimen axes Rate = 12.46703 A/sec Drift is low enough after shot 2 Measured defocus = -3.40 microns changed by -0.10 to target drift = 0.07 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -1.077 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.42.01_batch_tomo_08_15_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 118.1 In SetExposureForMean 120, change by a factor of 1.0158 would require too small a change in exposure time Image shift -0.256 0.335 IS units -1160.4 379.0 unbinned pixels; need stage -0.095 -0.427 if reset Tilting to -12 -12.00 degrees -7.4 0.8 unbinned pixels; 1.1 2.3 nm along two shift axes; -0.1 -2.6 nm on specimen axes Rate = 13.01337 A/sec Drift is low enough after shot 1 Measured defocus = -3.60 microns changed by 0.10 to target drift = 0.11 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -0.917 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.42.59_batch_tomo_08_15_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 118.1 In SetExposureForMean 120, change by a factor of 1.0162 would require too small a change in exposure time Image shift -0.293 0.395 IS units -1357.8 421.9 unbinned pixels; need stage -0.103 -0.505 if reset Tilting to -14 0.8 -0.4 unbinned pixels; 0.2 0.2 nm along two shift axes; 0.1 0.3 nm on specimen axes Rate = 1.59037 A/sec Drift is low enough after shot 1 Measured defocus = -3.57 microns changed by 0.07 to target drift = 0.08 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -0.822 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.43.55_batch_tomo_08_15_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 118.0 In SetExposureForMean 120, change by a factor of 1.0173 would require too small a change in exposure time Image shift -0.328 0.471 IS units -1599.1 446.2 unbinned pixels; need stage -0.104 -0.598 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 9.0 -7.8 unbinned pixels; 3.6 2.1 nm along two shift axes; 2.4 3.4 nm on specimen axes Rate = 20.87445 A/sec 6.9 1.4 unbinned pixels; 0.3 2.4 nm along two shift axes; -0.7 2.4 nm on specimen axes Rate = 12.31772 A/sec Drift is low enough after shot 2 Measured defocus = -3.45 microns changed by -0.05 to target drift = 0.07 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -1.137 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.45.01_batch_tomo_08_15_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 117.9 In SetExposureForMean 120, change by a factor of 1.0177 would require too small a change in exposure time Image shift -0.202 0.214 IS units -778.4 346.8 unbinned pixels; need stage -0.095 -0.288 if reset Tilting to -4 Correcting tilt backlash -12.4 2.1 unbinned pixels; 2.1 3.9 nm along two shift axes; -0.4 -4.4 nm on specimen axes Rate = 22.07676 A/sec -1.6 2.0 unbinned pixels; 0.9 0.3 nm along two shift axes; -0.7 -0.6 nm on specimen axes Rate = 4.49682 A/sec Drift is low enough after shot 2 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.08 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -1.201 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.46.12_batch_tomo_08_15_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 117.8 In SetExposureForMean 120, change by a factor of 1.0185 would require too small a change in exposure time Image shift -0.225 0.253 IS units -906.6 370.5 unbinned pixels; need stage -0.100 -0.333 if reset Tilting to -16 -16.00 degrees 1.0 -8.1 unbinned pixels; 2.8 0.6 nm along two shift axes; 2.8 0.6 nm on specimen axes Rate = 14.46178 A/sec -0.5 2.3 unbinned pixels; 0.8 0.1 nm along two shift axes; -0.8 -0.3 nm on specimen axes Rate = 4.1299 A/sec Drift is low enough after shot 2 Measured defocus = -3.61 microns changed by 0.11 to target drift = 0.00 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.01 nm/sec Defocus = -0.694 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.47.15_batch_tomo_08_15_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 117.9 In SetExposureForMean 120, change by a factor of 1.0181 would require too small a change in exposure time Image shift -0.269 0.319 IS units -1129.8 429.3 unbinned pixels; need stage -0.113 -0.430 if reset Tilting to -18 -1.8 -0.3 unbinned pixels; 0.1 0.6 nm along two shift axes; 0.2 -0.6 nm on specimen axes Rate = 3.23651 A/sec Drift is low enough after shot 1 Measured defocus = -3.59 microns changed by 0.09 to target drift = 0.08 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -0.577 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.48.12_batch_tomo_08_15_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 117.3 In SetExposureForMean 120, change by a factor of 1.0235 would require too small a change in exposure time Image shift -0.301 0.391 IS units -1356.6 445.9 unbinned pixels; need stage -0.112 -0.520 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 14.1 -0.1 unbinned pixels; 1.7 4.7 nm along two shift axes; -0.4 4.9 nm on specimen axes Rate = 24.73298 A/sec 7.5 0.1 unbinned pixels; 0.9 2.5 nm along two shift axes; -0.3 2.6 nm on specimen axes Rate = 13.20672 A/sec Drift is low enough after shot 2 Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.10 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -1.237 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.49.18_batch_tomo_08_15_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0223 would require too small a change in exposure time Image shift -0.223 0.239 IS units -866.3 378.6 unbinned pixels; need stage -0.104 -0.319 if reset Tilting to 0 Correcting tilt backlash -15.5 3.2 unbinned pixels; 2.9 4.7 nm along two shift axes; -0.7 -5.5 nm on specimen axes Rate = 27.69476 A/sec -11.1 1.9 unbinned pixels; 1.9 3.4 nm along two shift axes; -0.3 -3.9 nm on specimen axes Rate = 19.6767 A/sec -6.7 1.4 unbinned pixels; 1.3 2.0 nm along two shift axes; -0.3 -2.4 nm on specimen axes Rate = 11.96495 A/sec Drift is low enough after shot 3 Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.20 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.19 nm/sec Defocus = -1.278 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.50.28_batch_tomo_08_15_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 116.8 In SetExposureForMean 120, change by a factor of 1.0271 would require too small a change in exposure time Image shift -0.239 0.272 IS units -972.0 390.7 unbinned pixels; need stage -0.105 -0.356 if reset Tilting to -20 -20.00 degrees 0.9 -0.7 unbinned pixels; 0.3 0.2 nm along two shift axes; 0.2 0.3 nm on specimen axes Rate = 2.03395 A/sec Drift is low enough after shot 1 Measured defocus = -3.61 microns changed by 0.11 to target drift = 0.02 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.456 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.51.28_batch_tomo_08_15_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 117.3 In SetExposureForMean 120, change by a factor of 1.0244 would require too small a change in exposure time Image shift -0.294 0.350 IS units -1238.2 468.4 unbinned pixels; need stage -0.123 -0.482 if reset Tilting to -22 10.7 -8.1 unbinned pixels; 3.9 2.6 nm along two shift axes; 2.5 4.0 nm on specimen axes Rate = 23.50236 A/sec 5.3 1.8 unbinned pixels; 0.0 2.0 nm along two shift axes; -0.8 1.8 nm on specimen axes Rate = 9.82429 A/sec Drift is low enough after shot 2 Measured defocus = -3.59 microns changed by 0.09 to target drift = 0.14 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -0.359 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.52.30_batch_tomo_08_15_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 116.7 In SetExposureForMean 120, change by a factor of 1.0290 would require too small a change in exposure time Image shift -0.233 0.243 IS units -888.7 404.2 unbinned pixels; need stage -0.112 -0.353 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 4.5 -5.3 unbinned pixels; 2.3 0.9 nm along two shift axes; 1.7 1.7 nm on specimen axes Rate = 12.23461 A/sec Drift is low enough after shot 1 Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.06 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.295 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.53.31_batch_tomo_08_15_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 116.8 In SetExposureForMean 120, change by a factor of 1.0273 would require too small a change in exposure time Image shift -0.204 0.182 IS units -694.1 381.3 unbinned pixels; need stage -0.110 -0.258 if reset Tilting to 4 Correcting tilt backlash -14.5 0.9 unbinned pixels; 2.0 4.7 nm along two shift axes; 0.1 -5.1 nm on specimen axes Rate = 25.56503 A/sec -8.0 2.7 unbinned pixels; 1.8 2.3 nm along two shift axes; -0.7 -2.9 nm on specimen axes Rate = 14.82095 A/sec -6.0 2.3 unbinned pixels; 1.5 1.7 nm along two shift axes; -0.6 -2.2 nm on specimen axes Rate = 11.27579 A/sec Drift is low enough after shot 3 Measured defocus = -3.43 microns changed by -0.07 to target drift = 0.15 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.13 nm/sec Defocus = -1.379 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.54.41_batch_tomo_08_15_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, change by a factor of 1.0315 would require too small a change in exposure time Image shift -0.194 0.167 IS units -644.8 369.3 unbinned pixels; need stage -0.107 -0.241 if reset Tilting to -24 -24.00 degrees 0.9 0.4 unbinned pixels; 0.0 0.4 nm along two shift axes; -0.2 0.3 nm on specimen axes Rate = 1.78404 A/sec Drift is low enough after shot 1 Measured defocus = -3.64 microns changed by 0.14 to target drift = 0.06 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.219 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.55.41_batch_tomo_08_15_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 116.4 In SetExposureForMean 120, change by a factor of 1.0316 would require too small a change in exposure time Image shift -0.243 0.208 IS units -803.6 463.9 unbinned pixels; need stage -0.135 -0.327 if reset Tilting to -26 7.8 -7.8 unbinned pixels; 3.5 1.7 nm along two shift axes; 2.5 3.0 nm on specimen axes Rate = 19.37091 A/sec 7.2 2.6 unbinned pixels; 0.0 2.7 nm along two shift axes; -1.1 2.4 nm on specimen axes Rate = 13.42012 A/sec Drift is low enough after shot 2 Measured defocus = -3.58 microns changed by 0.08 to target drift = 0.15 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -0.153 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.56.44_batch_tomo_08_15_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 116.0 In SetExposureForMean 120, change by a factor of 1.0359 would require too small a change in exposure time Image shift -0.194 0.126 IS units -532.6 409.3 unbinned pixels; need stage -0.124 -0.224 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 2.4 -0.4 unbinned pixels; 0.4 0.7 nm along two shift axes; 0.1 0.8 nm on specimen axes Rate = 4.1799 A/sec Drift is low enough after shot 1 Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.08 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -1.408 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.57.46_batch_tomo_08_15_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, change by a factor of 1.0337 would require too small a change in exposure time Image shift -0.185 0.127 IS units -527.3 381.9 unbinned pixels; need stage -0.115 -0.200 if reset Tilting to 8 Correcting tilt backlash -14.7 6.1 unbinned pixels; 3.7 4.1 nm along two shift axes; -1.7 -5.3 nm on specimen axes Rate = 27.77624 A/sec -14.1 3.0 unbinned pixels; 2.7 4.3 nm along two shift axes; -0.6 -5.1 nm on specimen axes Rate = 25.36642 A/sec -7.9 4.7 unbinned pixels; 2.5 2.0 nm along two shift axes; -1.4 -2.9 nm on specimen axes Rate = 16.0372 A/sec -7.9 2.1 unbinned pixels; 1.6 2.4 nm along two shift axes; -0.5 -2.9 nm on specimen axes Rate = 14.41067 A/sec -6.3 2.6 unbinned pixels; 1.6 1.8 nm along two shift axes; -0.7 -2.3 nm on specimen axes Rate = 12.05094 A/sec Drift is low enough after shot 5 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.25 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.16 nm/sec Defocus = -1.478 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_00.59.08_batch_tomo_08_15_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 115.3 In SetExposureForMean 120, change by a factor of 1.0407 would require too small a change in exposure time Image shift -0.206 0.163 IS units -644.2 406.0 unbinned pixels; need stage -0.120 -0.243 if reset Tilting to -28 -28.00 degrees 1.6 0.2 unbinned pixels; 0.1 0.6 nm along two shift axes; -0.1 0.6 nm on specimen axes Rate = 2.84774 A/sec Drift is low enough after shot 1 Measured defocus = -3.59 microns changed by 0.09 to target drift = 0.23 nm/sec Measured defocus = -3.53 microns changed by 0.03 to target drift = 0.16 nm/sec Defocus = -0.035 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.00.07_batch_tomo_08_15_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 115.2 In SetExposureForMean 120, change by a factor of 1.0432 would require too small a change in exposure time Image shift -0.194 0.074 IS units -392.5 458.7 unbinned pixels; need stage -0.146 -0.174 if reset Tilting to -30 10.9 -8.4 unbinned pixels; 4.0 2.6 nm along two shift axes; 2.6 4.1 nm on specimen axes Rate = 24.10618 A/sec 5.8 2.1 unbinned pixels; 0.0 2.2 nm along two shift axes; -0.9 2.0 nm on specimen axes Rate = 10.9264 A/sec Drift is low enough after shot 2 Measured defocus = -3.58 microns changed by 0.08 to target drift = 0.06 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.10 nm/sec Defocus = 0.016 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.01.09_batch_tomo_08_15_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, change by a factor of 1.0501 would require too small a change in exposure time Image shift -0.133 -0.040 IS units -22.0 399.3 unbinned pixels; need stage -0.137 -0.024 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -0.1 -0.1 unbinned pixels; 0.0 0.0 nm along two shift axes; 0.1 -0.0 nm on specimen axes Rate = 0.29816 A/sec Drift is low enough after shot 1 Measured defocus = -3.43 microns changed by -0.07 to target drift = 0.06 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -1.542 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.02.12_batch_tomo_08_15_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, change by a factor of 1.0489 would require too small a change in exposure time Image shift -0.192 0.106 IS units -477.5 421.5 unbinned pixels; need stage -0.130 -0.185 if reset Tilting to 12 Correcting tilt backlash -23.4 8.4 unbinned pixels; 5.5 6.7 nm along two shift axes; -2.2 -8.5 nm on specimen axes Rate = 43.54328 A/sec -14.9 3.6 unbinned pixels; 2.9 4.5 nm along two shift axes; -0.8 -5.3 nm on specimen axes Rate = 26.83965 A/sec -6.6 3.4 unbinned pixels; 1.9 1.8 nm along two shift axes; -1.0 -2.4 nm on specimen axes Rate = 13.11678 A/sec Drift is low enough after shot 3 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.15 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.13 nm/sec Defocus = -1.635 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.03.22_batch_tomo_08_15_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 113.8 In SetExposureForMean 120, change by a factor of 1.0546 would require too small a change in exposure time Image shift -0.209 0.137 IS units -577.6 437.5 unbinned pixels; need stage -0.133 -0.223 if reset Tilting to -32 -32.00 degrees 9.1 -2.3 unbinned pixels; 1.8 2.7 nm along two shift axes; 0.5 3.3 nm on specimen axes Rate = 16.40779 A/sec 5.2 1.9 unbinned pixels; 0.0 1.9 nm along two shift axes; -0.8 1.8 nm on specimen axes Rate = 9.68961 A/sec Drift is low enough after shot 2 Measured defocus = -3.53 microns changed by 0.03 to target drift = 0.22 nm/sec Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.17 nm/sec Defocus = 0.087 um Dewars are NOT busy filling 9 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.04.34_batch_tomo_08_15_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 113.5 In SetExposureForMean 120, exposure time changed to 1.2000 Image shift -0.128 -0.106 IS units 160.6 450.2 unbinned pixels; need stage -0.160 0.050 if reset Tilting to -34 12.5 -7.1 unbinned pixels; 3.8 3.3 nm along two shift axes; 2.1 4.6 nm on specimen axes Rate = 25.20827 A/sec 8.5 1.2 unbinned pixels; 0.6 3.0 nm along two shift axes; -0.7 3.0 nm on specimen axes Rate = 15.13411 A/sec 6.3 1.2 unbinned pixels; 0.3 2.2 nm along two shift axes; -0.6 2.2 nm on specimen axes Rate = 11.2901 A/sec Drift is low enough after shot 3 Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.17 nm/sec Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.10 nm/sec Defocus = 0.089 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.05.42_batch_tomo_08_15_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 124.8 In SetExposureForMean 120, change by a factor of 0.9628 would require too small a change in exposure time Image shift -0.082 -0.182 IS units 413.2 396.3 unbinned pixels; need stage -0.149 0.161 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -7.2 0.3 unbinned pixels; 0.9 2.4 nm along two shift axes; 0.1 -2.5 nm on specimen axes Rate = 12.69409 A/sec Drift is low enough after shot 1 Measured defocus = -3.45 microns changed by -0.05 to target drift = 0.14 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.12 nm/sec Defocus = -1.682 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.06.44_batch_tomo_08_15_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 124.9 In SetExposureForMean 120, change by a factor of 0.9613 would require too small a change in exposure time Image shift -0.189 0.080 IS units -402.1 437.3 unbinned pixels; need stage -0.138 -0.161 if reset Tilting to 16 Correcting tilt backlash -8.4 -1.1 unbinned pixels; 0.6 2.9 nm along two shift axes; 0.7 -2.9 nm on specimen axes Rate = 14.88798 A/sec -2.8 5.4 unbinned pixels; 2.1 0.3 nm along two shift axes; -1.8 -1.2 nm on specimen axes Rate = 10.66507 A/sec Drift is low enough after shot 2 Measured defocus = -3.39 microns changed by -0.11 to target drift = 0.10 nm/sec Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.02 nm/sec Defocus = -1.731 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.07.54_batch_tomo_08_15_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 123.8 In SetExposureForMean 120, change by a factor of 0.9698 would require too small a change in exposure time Image shift -0.158 0.025 IS units -222.0 406.3 unbinned pixels; need stage -0.133 -0.095 if reset Tilting to -36 -36.00 degrees -0.6 -1.3 unbinned pixels; 0.4 0.4 nm along two shift axes; 0.5 -0.2 nm on specimen axes Rate = 2.54086 A/sec Drift is low enough after shot 1 Measured defocus = -3.57 microns changed by 0.07 to target drift = 0.15 nm/sec Measured defocus = -3.57 microns changed by 0.07 to target drift = 0.11 nm/sec Defocus = 0.232 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.08.55_batch_tomo_08_15_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 123.8 In SetExposureForMean 120, change by a factor of 0.9715 would require too small a change in exposure time Image shift -0.070 -0.243 IS units 590.5 421.4 unbinned pixels; need stage -0.163 0.241 if reset Tilting to -38 6.4 -7.7 unbinned pixels; 3.3 1.2 nm along two shift axes; 2.5 2.5 nm on specimen axes Rate = 17.51111 A/sec 11.0 1.3 unbinned pixels; 0.9 3.8 nm along two shift axes; -0.8 3.8 nm on specimen axes Rate = 19.36568 A/sec 8.7 0.2 unbinned pixels; 0.9 2.9 nm along two shift axes; -0.3 3.0 nm on specimen axes Rate = 15.20499 A/sec 2.5 0.0 unbinned pixels; 0.3 0.8 nm along two shift axes; -0.1 0.9 nm on specimen axes Rate = 4.37599 A/sec Drift is low enough after shot 4 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.21 nm/sec Measured defocus = -3.55 microns changed by 0.05 to target drift = 0.08 nm/sec Defocus = 0.224 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.10.08_batch_tomo_08_15_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 122.4 In SetExposureForMean 120, change by a factor of 0.9822 would require too small a change in exposure time Image shift -0.018 -0.329 IS units 874.2 362.1 unbinned pixels; need stage -0.152 0.377 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -12.6 3.6 unbinned pixels; 2.7 3.7 nm along two shift axes; -0.9 -4.5 nm on specimen axes Rate = 22.99819 A/sec -7.2 1.6 unbinned pixels; 1.4 2.2 nm along two shift axes; -0.4 -2.6 nm on specimen axes Rate = 12.895 A/sec Drift is low enough after shot 2 Measured defocus = -3.36 microns changed by -0.14 to target drift = 0.21 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.21 nm/sec Defocus = -1.860 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.11.17_batch_tomo_08_15_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 122.6 In SetExposureForMean 120, change by a factor of 0.9789 would require too small a change in exposure time Image shift -0.171 0.033 IS units -259.0 435.2 unbinned pixels; need stage -0.142 -0.111 if reset Tilting to 20 Correcting tilt backlash 0.8 -1.1 unbinned pixels; 0.5 0.1 nm along two shift axes; 0.4 0.3 nm on specimen axes Rate = 2.49328 A/sec Drift is low enough after shot 1 Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.15 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.09 nm/sec Defocus = -1.925 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.12.16_batch_tomo_08_15_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 121.0 In SetExposureForMean 120, change by a factor of 0.9918 would require too small a change in exposure time Image shift -0.132 -0.067 IS units 51.5 422.2 unbinned pixels; need stage -0.147 0.005 if reset Tilting to -40 -40.00 degrees 2.5 -1.1 unbinned pixels; 0.7 0.7 nm along two shift axes; 0.3 0.9 nm on specimen axes Rate = 4.88598 A/sec Drift is low enough after shot 1 Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.08 nm/sec Measured defocus = -3.55 microns changed by 0.05 to target drift = 0.10 nm/sec Defocus = 0.265 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.13.18_batch_tomo_08_15_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 120.8 In SetExposureForMean 120, change by a factor of 0.9954 would require too small a change in exposure time Image shift -0.004 -0.384 IS units 1034.8 376.6 unbinned pixels; need stage -0.161 0.462 if reset Tilting to -42 7.3 -6.3 unbinned pixels; 2.9 1.7 nm along two shift axes; 2.0 2.8 nm on specimen axes Rate = 16.89652 A/sec 6.0 1.6 unbinned pixels; 0.2 2.2 nm along two shift axes; -0.7 2.0 nm on specimen axes Rate = 10.89806 A/sec Drift is low enough after shot 2 Measured defocus = -3.61 microns changed by 0.11 to target drift = 0.18 nm/sec Measured defocus = -3.38 microns changed by -0.12 to target drift = 0.08 nm/sec Defocus = 0.254 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.14.20_batch_tomo_08_15_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0081 would require too small a change in exposure time Image shift 0.029 -0.428 IS units 1187.2 328.4 unbinned pixels; need stage -0.150 0.551 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -13.0 1.4 unbinned pixels; 2.0 4.2 nm along two shift axes; -0.1 -4.6 nm on specimen axes Rate = 22.97672 A/sec -9.1 2.0 unbinned pixels; 1.7 2.8 nm along two shift axes; -0.4 -3.2 nm on specimen axes Rate = 16.28157 A/sec -11.8 2.1 unbinned pixels; 2.1 3.7 nm along two shift axes; -0.4 -4.2 nm on specimen axes Rate = 21.07057 A/sec -7.8 1.7 unbinned pixels; 1.5 2.4 nm along two shift axes; -0.4 -2.8 nm on specimen axes Rate = 14.03458 A/sec -2.4 0.8 unbinned pixels; 0.6 0.7 nm along two shift axes; -0.2 -0.9 nm on specimen axes Rate = 4.51206 A/sec Drift is low enough after shot 5 Measured defocus = -3.32 microns changed by -0.18 to target drift = 0.05 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.20 nm/sec Defocus = -2.099 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.15.46_batch_tomo_08_15_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 119.8 In SetExposureForMean 120, change by a factor of 1.0022 would require too small a change in exposure time Image shift -0.156 -0.004 IS units -143.2 427.1 unbinned pixels; need stage -0.143 -0.070 if reset Tilting to 24 Correcting tilt backlash -8.9 0.7 unbinned pixels; 1.3 2.9 nm along two shift axes; 0.0 -3.1 nm on specimen axes Rate = 15.61689 A/sec -1.9 6.3 unbinned pixels; 2.3 0.1 nm along two shift axes; -2.2 -0.9 nm on specimen axes Rate = 11.62373 A/sec Drift is low enough after shot 2 Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.09 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.10 nm/sec Defocus = -2.137 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.16.51_batch_tomo_08_15_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 117.6 In SetExposureForMean 120, change by a factor of 1.0200 would require too small a change in exposure time Image shift -0.128 -0.107 IS units 163.0 449.3 unbinned pixels; need stage -0.160 0.047 if reset Tilting to -44 -44.00 degrees 15.8 -2.6 unbinned pixels; 2.7 4.9 nm along two shift axes; 0.4 5.6 nm on specimen axes Rate = 28.0268 A/sec 9.8 0.6 unbinned pixels; 0.9 3.3 nm along two shift axes; -0.5 3.4 nm on specimen axes Rate = 17.2411 A/sec 6.7 1.0 unbinned pixels; 0.4 2.3 nm along two shift axes; -0.6 2.3 nm on specimen axes Rate = 11.92646 A/sec Drift is low enough after shot 3 Measured defocus = -3.61 microns changed by 0.11 to target drift = 0.21 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.21 nm/sec Defocus = 0.371 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.18.09_batch_tomo_08_15_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 117.7 In SetExposureForMean 120, change by a factor of 1.0210 would require too small a change in exposure time Image shift 0.041 -0.490 IS units 1369.4 354.7 unbinned pixels; need stage -0.164 0.657 if reset Tilting to -46 7.8 -4.1 unbinned pixels; 2.3 2.1 nm along two shift axes; 1.2 2.9 nm on specimen axes Rate = 15.40407 A/sec 7.9 1.8 unbinned pixels; 0.3 2.8 nm along two shift axes; -0.9 2.7 nm on specimen axes Rate = 14.29589 A/sec 5.8 0.8 unbinned pixels; 0.4 2.0 nm along two shift axes; -0.5 2.0 nm on specimen axes Rate = 10.33738 A/sec Drift is low enough after shot 3 Measured defocus = -3.55 microns changed by 0.05 to target drift = 0.17 nm/sec Measured defocus = -3.45 microns changed by -0.05 to target drift = 0.14 nm/sec Defocus = 0.370 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.19.17_batch_tomo_08_15_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 115.9 In SetExposureForMean 120, change by a factor of 1.0368 would require too small a change in exposure time Image shift 0.062 -0.517 IS units 1462.9 322.9 unbinned pixels; need stage -0.156 0.730 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -20.1 3.9 unbinned pixels; 3.7 6.2 nm along two shift axes; -0.8 -7.2 nm on specimen axes Rate = 35.90927 A/sec -7.2 3.1 unbinned pixels; 1.9 2.0 nm along two shift axes; -0.9 -2.6 nm on specimen axes Rate = 13.79211 A/sec Drift is low enough after shot 2 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.37 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.34 nm/sec Defocus = -2.219 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.20.32_batch_tomo_08_15_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 115.9 In SetExposureForMean 120, change by a factor of 1.0353 would require too small a change in exposure time Image shift -0.153 -0.028 IS units -73.8 442.2 unbinned pixels; need stage -0.150 -0.045 if reset Tilting to 28 Correcting tilt backlash -11.6 -1.1 unbinned pixels; 1.0 4.0 nm along two shift axes; 0.7 -4.0 nm on specimen axes Rate = 20.44525 A/sec -4.2 5.1 unbinned pixels; 2.2 0.8 nm along two shift axes; -1.7 -1.6 nm on specimen axes Rate = 11.54896 A/sec Drift is low enough after shot 2 Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.24 nm/sec Measured defocus = -3.53 microns changed by 0.03 to target drift = 0.16 nm/sec Defocus = -2.187 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.21.36_batch_tomo_08_15_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 114.0 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift -0.122 -0.135 IS units 244.6 461.2 unbinned pixels; need stage -0.166 0.081 if reset Tilting to -48 -48.00 degrees 17.4 -7.2 unbinned pixels; 4.4 4.9 nm along two shift axes; 2.0 6.3 nm on specimen axes Rate = 32.89005 A/sec 4.3 2.9 unbinned pixels; 0.5 1.8 nm along two shift axes; -1.2 1.4 nm on specimen axes Rate = 9.20321 A/sec Drift is low enough after shot 2 Measured defocus = -3.59 microns changed by 0.09 to target drift = 0.28 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.16 nm/sec Defocus = 0.479 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.22.45_batch_tomo_08_15_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 125.1 In SetExposureForMean 120, change by a factor of 0.9602 would require too small a change in exposure time Image shift 0.101 -0.619 IS units 1775.8 313.0 unbinned pixels; need stage -0.162 0.923 if reset Tilting to -50 10.0 -6.4 unbinned pixels; 3.3 2.6 nm along two shift axes; 1.9 3.7 nm on specimen axes Rate = 20.83849 A/sec 9.2 3.1 unbinned pixels; 0.0 3.4 nm along two shift axes; -1.4 3.1 nm on specimen axes Rate = 17.01424 A/sec 5.7 1.0 unbinned pixels; 0.3 2.0 nm along two shift axes; -0.5 2.0 nm on specimen axes Rate = 10.22984 A/sec Drift is low enough after shot 3 Measured defocus = -3.58 microns changed by 0.08 to target drift = 0.23 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.27 nm/sec Defocus = 0.553 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.23.53_batch_tomo_08_15_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 122.6 In SetExposureForMean 120, change by a factor of 0.9787 would require too small a change in exposure time Image shift 0.136 -0.684 IS units 1987.5 279.8 unbinned pixels; need stage -0.157 1.079 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -17.2 5.8 unbinned pixels; 3.9 5.0 nm along two shift axes; -1.5 -6.2 nm on specimen axes Rate = 31.8176 A/sec -13.7 4.0 unbinned pixels; 2.9 4.1 nm along two shift axes; -1.0 -4.9 nm on specimen axes Rate = 25.0535 A/sec -12.8 2.9 unbinned pixels; 2.5 3.9 nm along two shift axes; -0.6 -4.6 nm on specimen axes Rate = 23.07977 A/sec -10.1 2.4 unbinned pixels; 2.0 3.0 nm along two shift axes; -0.5 -3.6 nm on specimen axes Rate = 18.10696 A/sec -9.4 1.8 unbinned pixels; 1.7 2.9 nm along two shift axes; -0.3 -3.4 nm on specimen axes Rate = 16.79934 A/sec -8.0 1.9 unbinned pixels; 1.6 2.4 nm along two shift axes; -0.4 -2.9 nm on specimen axes Rate = 14.3616 A/sec -7.0 1.7 unbinned pixels; 1.4 2.1 nm along two shift axes; -0.4 -2.5 nm on specimen axes Rate = 12.69748 A/sec Drift is low enough after shot 7 Measured defocus = -3.41 microns changed by -0.09 to target drift = 0.23 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.28 nm/sec Defocus = -2.289 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.25.31_batch_tomo_08_15_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 122.8 In SetExposureForMean 120, change by a factor of 0.9766 would require too small a change in exposure time Image shift -0.138 -0.100 IS units 134.6 471.8 unbinned pixels; need stage -0.166 0.037 if reset Tilting to 32 Correcting tilt backlash -13.9 -4.1 unbinned pixels; 0.3 5.1 nm along two shift axes; 1.8 -4.7 nm on specimen axes Rate = 25.42318 A/sec -6.7 3.2 unbinned pixels; 1.8 1.8 nm along two shift axes; -0.9 -2.5 nm on specimen axes Rate = 13.02456 A/sec Drift is low enough after shot 2 Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.28 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.20 nm/sec Defocus = -2.289 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.26.36_batch_tomo_08_15_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 120.3 In SetExposureForMean 120, change by a factor of 0.9969 would require too small a change in exposure time Image shift -0.091 -0.224 IS units 515.8 461.6 unbinned pixels; need stage -0.175 0.197 if reset Tilting to -52 -52.00 degrees 27.6 -6.7 unbinned pixels; 5.5 8.3 nm along two shift axes; 1.5 9.9 nm on specimen axes Rate = 49.80714 A/sec 4.3 1.8 unbinned pixels; 0.1 1.6 nm along two shift axes; -0.8 1.5 nm on specimen axes Rate = 8.23412 A/sec Drift is low enough after shot 2 Measured defocus = -3.68 microns changed by 0.18 to target drift = 0.27 nm/sec Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.32 nm/sec Defocus = 0.692 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.27.45_batch_tomo_08_15_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 119.9 In SetExposureForMean 120, change by a factor of 1.0001 would require too small a change in exposure time Image shift 0.186 -0.785 IS units 2309.5 241.7 unbinned pixels; need stage -0.154 1.314 if reset Tilting to -54 9.2 -6.6 unbinned pixels; 3.3 2.2 nm along two shift axes; 2.0 3.4 nm on specimen axes Rate = 19.80441 A/sec 8.2 3.3 unbinned pixels; 0.1 3.1 nm along two shift axes; -1.4 2.8 nm on specimen axes Rate = 15.46652 A/sec 9.2 0.4 unbinned pixels; 0.9 3.1 nm along two shift axes; -0.4 3.2 nm on specimen axes Rate = 16.08349 A/sec 3.6 0.8 unbinned pixels; 0.1 1.3 nm along two shift axes; -0.4 1.2 nm on specimen axes Rate = 6.44929 A/sec Drift is low enough after shot 4 Measured defocus = -3.64 microns changed by 0.14 to target drift = 0.10 nm/sec Measured defocus = -3.35 microns changed by -0.15 to target drift = 0.31 nm/sec Defocus = 0.675 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.29.05_batch_tomo_08_15_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 116.9 In SetExposureForMean 120, change by a factor of 1.0260 would require too small a change in exposure time Image shift 0.217 -0.852 IS units 2522.3 219.3 unbinned pixels; need stage -0.153 1.505 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -24.1 5.0 unbinned pixels; 4.5 7.4 nm along two shift axes; -1.0 -8.6 nm on specimen axes Rate = 43.20722 A/sec -12.5 2.9 unbinned pixels; 2.4 3.8 nm along two shift axes; -0.6 -4.5 nm on specimen axes Rate = 22.48502 A/sec -7.6 3.8 unbinned pixels; 2.2 2.1 nm along two shift axes; -1.1 -2.8 nm on specimen axes Rate = 14.91028 A/sec -8.1 1.8 unbinned pixels; 1.5 2.5 nm along two shift axes; -0.4 -2.9 nm on specimen axes Rate = 14.57148 A/sec -10.2 1.9 unbinned pixels; 1.8 3.2 nm along two shift axes; -0.3 -3.6 nm on specimen axes Rate = 18.16779 A/sec -9.0 2.0 unbinned pixels; 1.7 2.7 nm along two shift axes; -0.4 -3.2 nm on specimen axes Rate = 16.15593 A/sec -3.7 1.3 unbinned pixels; 0.9 1.1 nm along two shift axes; -0.3 -1.3 nm on specimen axes Rate = 6.90771 A/sec Drift is low enough after shot 7 Measured defocus = -3.31 microns changed by -0.19 to target drift = 0.05 nm/sec Measured defocus = -3.57 microns changed by 0.07 to target drift = 0.29 nm/sec Defocus = -2.407 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.30.44_batch_tomo_08_15_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 117.0 In SetExposureForMean 120, change by a factor of 1.0250 would require too small a change in exposure time Image shift -0.082 -0.205 IS units 475.1 418.4 unbinned pixels; need stage -0.159 0.186 if reset Tilting to 36 Correcting tilt backlash -7.8 -4.0 unbinned pixels; 0.4 3.0 nm along two shift axes; 1.6 -2.6 nm on specimen axes Rate = 15.39702 A/sec -2.1 2.2 unbinned pixels; 1.0 0.4 nm along two shift axes; -0.7 -0.8 nm on specimen axes Rate = 5.44692 A/sec Drift is low enough after shot 2 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -2.336 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.31.54_batch_tomo_08_15_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.046 -0.307 IS units 786.8 418.4 unbinned pixels; need stage -0.168 0.327 if reset Tilting to -56 -56.00 degrees 12.8 -6.2 unbinned pixels; 3.6 3.5 nm along two shift axes; 1.8 4.7 nm on specimen axes Rate = 24.99783 A/sec 6.8 2.7 unbinned pixels; 0.1 2.6 nm along two shift axes; -1.2 2.3 nm on specimen axes Rate = 12.83107 A/sec Drift is low enough after shot 2 Measured defocus = -3.69 microns changed by 0.19 to target drift = 0.13 nm/sec Measured defocus = -3.37 microns changed by -0.13 to target drift = 0.09 nm/sec Defocus = 0.736 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.33.03_batch_tomo_08_15_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 124.0 In SetExposureForMean 120, change by a factor of 0.9658 would require too small a change in exposure time Image shift 0.246 -0.907 IS units 2699.1 192.5 unbinned pixels; need stage -0.149 1.695 if reset Tilting to -58 6.1 -7.1 unbinned pixels; 3.1 1.2 nm along two shift axes; 2.3 2.3 nm on specimen axes Rate = 16.34085 A/sec 5.3 3.7 unbinned pixels; 0.6 2.2 nm along two shift axes; -1.4 1.7 nm on specimen axes Rate = 11.30416 A/sec Drift is low enough after shot 2 Measured defocus = -3.58 microns changed by 0.08 to target drift = 0.17 nm/sec Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.08 nm/sec Defocus = 0.772 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.34.11_batch_tomo_08_15_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 120.0 In SetExposureForMean 120, change by a factor of 0.9962 would require too small a change in exposure time Image shift 0.287 -0.985 IS units 2951.5 155.4 unbinned pixels; need stage -0.144 1.960 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -20.9 1.9 unbinned pixels; 3.1 6.7 nm along two shift axes; -0.0 -7.4 nm on specimen axes Rate = 36.84465 A/sec -15.2 6.1 unbinned pixels; 3.8 4.3 nm along two shift axes; -1.7 -5.5 nm on specimen axes Rate = 28.67672 A/sec -8.8 2.9 unbinned pixels; 2.0 2.6 nm along two shift axes; -0.7 -3.2 nm on specimen axes Rate = 16.25379 A/sec -7.6 2.9 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.8 -2.8 nm on specimen axes Rate = 14.29651 A/sec -9.4 3.0 unbinned pixels; 2.1 2.8 nm along two shift axes; -0.8 -3.4 nm on specimen axes Rate = 17.3567 A/sec -7.1 1.8 unbinned pixels; 1.4 2.1 nm along two shift axes; -0.4 -2.5 nm on specimen axes Rate = 12.76488 A/sec Drift is low enough after shot 6 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.16 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.18 nm/sec Defocus = -2.438 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.35.49_batch_tomo_08_15_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 120.9 In SetExposureForMean 120, change by a factor of 0.9903 would require too small a change in exposure time Image shift -0.029 -0.302 IS units 789.0 366.3 unbinned pixels; need stage -0.150 0.339 if reset Tilting to 40 Correcting tilt backlash -14.5 -4.4 unbinned pixels; 0.2 5.3 nm along two shift axes; 2.0 -4.9 nm on specimen axes Rate = 26.55781 A/sec -5.4 2.6 unbinned pixels; 1.5 1.5 nm along two shift axes; -0.7 -2.0 nm on specimen axes Rate = 10.47144 A/sec Drift is low enough after shot 2 Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.09 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -2.424 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.36.53_batch_tomo_08_15_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 116.6 In SetExposureForMean 120, change by a factor of 1.0253 would require too small a change in exposure time Image shift 0.013 -0.419 IS units 1146.3 363.9 unbinned pixels; need stage -0.160 0.514 if reset Tilting to -60 -60.00 degrees 12.8 -7.0 unbinned pixels; 3.8 3.4 nm along two shift axes; 2.1 4.7 nm on specimen axes Rate = 25.50359 A/sec 5.0 2.3 unbinned pixels; 0.2 1.9 nm along two shift axes; -1.0 1.7 nm on specimen axes Rate = 9.71627 A/sec Drift is low enough after shot 2 Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.24 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.14 nm/sec Defocus = 0.786 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.38.03_batch_tomo_08_15_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 116.0 In SetExposureForMean 120, change by a factor of 1.0282 would require too small a change in exposure time Image shift 0.330 -1.076 IS units 3240.8 125.7 unbinned pixels; need stage -0.143 2.284 if reset Tilting to -62 12.3 -7.1 unbinned pixels; 3.8 3.2 nm along two shift axes; 2.1 4.5 nm on specimen axes Rate = 24.82699 A/sec 5.7 3.6 unbinned pixels; 0.5 2.3 nm along two shift axes; -1.4 1.9 nm on specimen axes Rate = 11.93418 A/sec Drift is low enough after shot 2 Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.12 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.15 nm/sec Defocus = 0.815 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.39.07_batch_tomo_08_15_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 111.6 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.374 -1.157 IS units 3503.4 84.4 unbinned pixels; need stage -0.137 2.633 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -25.7 0.6 unbinned pixels; 3.2 8.4 nm along two shift axes; 0.6 -9.0 nm on specimen axes Rate = 45.14023 A/sec -15.0 8.4 unbinned pixels; 4.5 3.9 nm along two shift axes; -2.5 -5.5 nm on specimen axes Rate = 30.05435 A/sec -12.8 5.4 unbinned pixels; 3.3 3.6 nm along two shift axes; -1.5 -4.7 nm on specimen axes Rate = 24.31624 A/sec -9.1 2.9 unbinned pixels; 2.0 2.7 nm along two shift axes; -0.8 -3.3 nm on specimen axes Rate = 16.70432 A/sec -10.7 3.2 unbinned pixels; 2.3 3.2 nm along two shift axes; -0.8 -3.9 nm on specimen axes Rate = 19.64415 A/sec -7.7 2.6 unbinned pixels; 1.8 2.2 nm along two shift axes; -0.7 -2.8 nm on specimen axes Rate = 14.1813 A/sec -7.6 1.1 unbinned pixels; 1.2 2.4 nm along two shift axes; -0.1 -2.7 nm on specimen axes Rate = 13.43195 A/sec Drift is low enough after shot 7 Measured defocus = -3.37 microns changed by -0.13 to target drift = 0.17 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.30 nm/sec Defocus = -2.572 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.40.46_batch_tomo_08_15_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 121.5 In SetExposureForMean 120, change by a factor of 0.9824 would require too small a change in exposure time Image shift 0.041 -0.440 IS units 1232.9 306.7 unbinned pixels; need stage -0.143 0.573 if reset Tilting to 44 Correcting tilt backlash -13.2 1.0 unbinned pixels; 1.9 4.2 nm along two shift axes; 0.1 -4.7 nm on specimen axes Rate = 23.17954 A/sec -6.9 2.9 unbinned pixels; 1.8 1.9 nm along two shift axes; -0.8 -2.5 nm on specimen axes Rate = 13.07569 A/sec Drift is low enough after shot 2 Measured defocus = -3.53 microns changed by 0.03 to target drift = 0.27 nm/sec Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.33 nm/sec Defocus = -2.575 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.41.50_batch_tomo_08_15_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 116.5 In SetExposureForMean 120, change by a factor of 1.0236 would require too small a change in exposure time Image shift 0.049 -0.487 IS units 1366.2 330.0 unbinned pixels; need stage -0.156 0.657 if reset Tilting to -64 -64.00 degrees -9.3 -3.3 unbinned pixels; 0.0 3.5 nm along two shift axes; 1.5 -3.2 nm on specimen axes Rate = 17.46119 A/sec 2.6 3.2 unbinned pixels; 0.7 1.3 nm along two shift axes; -1.2 0.8 nm on specimen axes Rate = 7.29178 A/sec Drift is low enough after shot 2 Measured defocus = -3.41 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -3.37 microns changed by -0.13 to target drift = 0.14 nm/sec Defocus = 0.602 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.43.00_batch_tomo_08_15_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 115.4 In SetExposureForMean 120, change by a factor of 1.0277 would require too small a change in exposure time Image shift 0.396 -1.220 IS units 3695.1 84.2 unbinned pixels; need stage -0.143 2.974 if reset Tilting to -66 11.7 -7.9 unbinned pixels; 4.0 2.9 nm along two shift axes; 2.4 4.3 nm on specimen axes Rate = 24.78369 A/sec 2.9 3.7 unbinned pixels; 0.9 1.4 nm along two shift axes; -1.4 0.9 nm on specimen axes Rate = 8.31716 A/sec Drift is low enough after shot 2 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.20 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.09 nm/sec Defocus = 0.685 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.44.03_batch_tomo_08_15_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 109.5 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.452 -1.327 IS units 4040.0 32.1 unbinned pixels; need stage -0.135 3.510 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -17.1 1.0 unbinned pixels; 2.3 5.6 nm along two shift axes; 0.2 -6.0 nm on specimen axes Rate = 30.08933 A/sec -9.4 7.1 unbinned pixels; 3.4 2.3 nm along two shift axes; -2.2 -3.5 nm on specimen axes Rate = 20.66377 A/sec -7.6 4.1 unbinned pixels; 2.2 2.0 nm along two shift axes; -1.2 -2.8 nm on specimen axes Rate = 15.173 A/sec -6.0 2.8 unbinned pixels; 1.6 1.6 nm along two shift axes; -0.8 -2.2 nm on specimen axes Rate = 11.54317 A/sec Drift is low enough after shot 4 Measured defocus = -3.37 microns changed by -0.13 to target drift = 0.26 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.11 nm/sec Defocus = -2.697 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.45.26_batch_tomo_08_15_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 119.8 In SetExposureForMean 120, change by a factor of 0.9936 would require too small a change in exposure time Image shift 0.070 -0.505 IS units 1437.2 288.8 unbinned pixels; need stage -0.144 0.718 if reset Tilting to 48 Correcting tilt backlash -10.3 -1.9 unbinned pixels; 0.6 3.6 nm along two shift axes; 1.0 -3.6 nm on specimen axes Rate = 18.45112 A/sec -2.0 4.6 unbinned pixels; 1.8 0.1 nm along two shift axes; -1.6 -0.9 nm on specimen axes Rate = 8.84424 A/sec Drift is low enough after shot 2 Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.08 nm/sec Measured defocus = -3.58 microns changed by 0.08 to target drift = 0.16 nm/sec Defocus = -2.594 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.46.31_batch_tomo_08_15_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 112.8 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.094 -0.579 IS units 1661.5 293.8 unbinned pixels; need stage -0.152 0.864 if reset Tilting to -68 -68.00 degrees 8.8 -6.5 unbinned pixels; 3.2 2.1 nm along two shift axes; 2.0 3.3 nm on specimen axes Rate = 19.13622 A/sec 7.0 1.7 unbinned pixels; 0.3 2.5 nm along two shift axes; -0.8 2.4 nm on specimen axes Rate = 12.71356 A/sec Drift is low enough after shot 2 Measured defocus = -3.43 microns changed by -0.07 to target drift = 0.01 nm/sec Measured defocus = -3.55 microns changed by 0.05 to target drift = 0.11 nm/sec Defocus = 0.658 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.47.42_batch_tomo_08_15_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 119.2 In SetExposureForMean 120, change by a factor of 0.9883 would require too small a change in exposure time Image shift 0.492 -1.414 IS units 4314.7 6.6 unbinned pixels; need stage -0.135 4.072 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 0.56 microns changed by -4.06 to target drift = 0.52 nm/sec Focus area changed from stored state, axis position -2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_09.st Running script 1 at item 17 (-382.58, -439.82) 01:48:39 Refine ZLP: additional offset = -0.4 eV; total offset = -14.1 eV Item 17: Stage: -382.58 -439.82 -223 Label: 17 Note: Sec 16 - anchor_maps.map Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Target defocus is: -3.50 um Target defocus is: -3.75 um Target defocus = -3.75 Angle = -4.40, increment = 0.60, shift = -0.3 -1.4 pixels, -0.001 um Y Angle = -3.20, increment = 1.80, shift = -7.6 -4.7 pixels, -0.018 um Y Angle = 0.41, increment = 5.41, shift = -34.4 -8.4 pixels, -0.080 um Y Angle = 3.00, increment = 8.00, shift = -9.2 -8.6 pixels, -0.023 um Y Rough eucentricity: changing Z by 0.16 to -223.22, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Angle = 18.00, shift = -7.7, 9.2 pixels, 0.023, -0.015 um, IS = -0.023, 0.015 Angle = 11.00, shift = -52.2, -6.6 pixels, -0.002, -0.120 um, IS = -0.021, 0.135 Angle = 4.00, shift = -37.4, 0.5 pixels, 0.011, -0.085 um, IS = -0.032, 0.220 Angle = -4.00, shift = 3.8, -16.5 pixels, -0.038, 0.004 um, IS = 0.007, 0.216 Angle = -11.00, shift = -72.9, -16.9 pixels, -0.020, -0.170 um, IS = 0.026, 0.385 Angle = -18.00, shift = -26.6, 1.2 pixels, 0.010, -0.060 um, IS = 0.017, 0.445 Angle = -24.00, shift = 32.6, 7.3 pixels, 0.008, 0.076 um, IS = 0.009, 0.370 General solution delZ 0.55, delY 0.32, yZero 0.25 solution with assumed YZero 0.22: delZ 0.55, delY -0.07 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.55 microns and lateral displacement of -0.32 microns Disparity in stage position after aligning to center of map frame: 0.01 0.08 Aligning to target with image shift equivalent to stage shift: 0.01 0.07 Disparity in stage position after aligning to map item itself: -0.00 0.07 (align shift -0.02 -0.04) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -382.57 -439.79 -223.75 -0.6 -0.1 unbinned pixels; 0.0 0.2 nm along two shift axes; 0.1 -0.2 nm on specimen axes Rate = 1.07185 A/sec Drift is low enough after shot 1 Measured defocus = 1.03 microns changed by -4.78 to target drift = 0.07 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.03 nm/sec Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.04 nm/sec Defocus = -0.869 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.57.35_batch_tomo_09_17_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 114.5 In SetExposureForMean 120, change by a factor of 1.0484 would require too small a change in exposure time Image shift 0.004 -0.037 IS units 103.9 22.8 unbinned pixels; need stage -0.011 0.037 if reset Tilting to -8 Correcting tilt backlash 7.1 0.4 unbinned pixels; 0.7 2.4 nm along two shift axes; -0.4 2.5 nm on specimen axes Rate = 12.4247 A/sec Drift is low enough after shot 1 Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.881 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.58.40_batch_tomo_09_17_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 114.1 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift -0.017 -0.062 IS units 151.5 106.6 unbinned pixels; need stage -0.041 0.051 if reset Tilting to -12 -12.00 degrees -8.4 1.5 unbinned pixels; 1.5 2.6 nm along two shift axes; -0.3 -3.0 nm on specimen axes Rate = 15.03129 A/sec -3.9 4.9 unbinned pixels; 2.1 0.7 nm along two shift axes; -1.6 -1.5 nm on specimen axes Rate = 10.97326 A/sec Drift is low enough after shot 2 Measured defocus = -3.85 microns changed by 0.10 to target drift = 0.09 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -0.756 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_01.59.43_batch_tomo_09_17_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 126.1 In SetExposureForMean 120, exposure time changed to 1.2000 Image shift -0.077 0.048 IS units -205.3 164.0 unbinned pixels; need stage -0.050 -0.080 if reset Tilting to -14 1.1 0.1 unbinned pixels; 0.1 0.4 nm along two shift axes; -0.1 0.4 nm on specimen axes Rate = 1.87861 A/sec Drift is low enough after shot 1 Measured defocus = -3.87 microns changed by 0.12 to target drift = 0.08 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -0.612 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.00.40_batch_tomo_09_17_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 114.8 In SetExposureForMean 120, change by a factor of 1.0457 would require too small a change in exposure time Image shift -0.111 0.116 IS units -422.7 193.2 unbinned pixels; need stage -0.053 -0.160 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 6.9 -1.5 unbinned pixels; 1.3 2.1 nm along two shift axes; 0.3 2.5 nm on specimen axes Rate = 12.32134 A/sec Drift is low enough after shot 1 Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.10 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = -0.858 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.01.40_batch_tomo_09_17_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 114.1 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift -0.003 -0.110 IS units 296.4 113.0 unbinned pixels; need stage -0.048 0.102 if reset Tilting to -4 Correcting tilt backlash -8.2 0.2 unbinned pixels; 1.0 2.7 nm along two shift axes; 0.2 -2.9 nm on specimen axes Rate = 14.45466 A/sec -4.8 4.9 unbinned pixels; 2.2 1.0 nm along two shift axes; -1.6 -1.8 nm on specimen axes Rate = 11.98652 A/sec Drift is low enough after shot 2 Measured defocus = -3.69 microns changed by -0.06 to target drift = 0.16 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.12 nm/sec Defocus = -0.897 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.02.45_batch_tomo_09_17_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 125.1 In SetExposureForMean 120, change by a factor of 0.9593 would require too small a change in exposure time Image shift -0.040 -0.036 IS units 57.0 142.5 unbinned pixels; need stage -0.051 0.016 if reset Tilting to -16 -16.00 degrees -1.1 -0.1 unbinned pixels; 0.1 0.4 nm along two shift axes; 0.1 -0.4 nm on specimen axes Rate = 1.98103 A/sec Drift is low enough after shot 1 Measured defocus = -3.86 microns changed by 0.11 to target drift = 0.04 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = -0.489 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.03.54_batch_tomo_09_17_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 126.3 In SetExposureForMean 120, exposure time changed to 1.2000 Image shift -0.149 0.151 IS units -554.8 261.8 unbinned pixels; need stage -0.073 -0.213 if reset Tilting to -18 10.7 -7.6 unbinned pixels; 3.8 2.6 nm along two shift axes; 2.3 4.0 nm on specimen axes Rate = 23.09659 A/sec 6.4 1.5 unbinned pixels; 0.3 2.3 nm along two shift axes; -0.7 2.2 nm on specimen axes Rate = 11.48811 A/sec Drift is low enough after shot 2 Measured defocus = -3.83 microns changed by 0.08 to target drift = 0.14 nm/sec Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.10 nm/sec Defocus = -0.379 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.04.57_batch_tomo_09_17_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 114.9 In SetExposureForMean 120, change by a factor of 1.0449 would require too small a change in exposure time Image shift -0.091 0.045 IS units -212.6 204.6 unbinned pixels; need stage -0.064 -0.086 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 5.9 1.1 unbinned pixels; 0.3 2.1 nm along two shift axes; -0.5 2.0 nm on specimen axes Rate = 10.55395 A/sec Drift is low enough after shot 1 Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.03 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.891 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.05.58_batch_tomo_09_17_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 113.4 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift -0.051 -0.037 IS units 49.3 174.3 unbinned pixels; need stage -0.061 0.012 if reset Tilting to 0 Correcting tilt backlash -12.4 1.8 unbinned pixels; 2.0 3.9 nm along two shift axes; -0.2 -4.4 nm on specimen axes Rate = 21.97392 A/sec -6.8 4.8 unbinned pixels; 2.4 1.7 nm along two shift axes; -1.5 -2.5 nm on specimen axes Rate = 14.49839 A/sec -4.2 1.3 unbinned pixels; 0.9 1.2 nm along two shift axes; -0.3 -1.5 nm on specimen axes Rate = 7.69921 A/sec Drift is low enough after shot 3 Measured defocus = -3.69 microns changed by -0.06 to target drift = 0.19 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.23 nm/sec Defocus = -0.935 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.07.09_batch_tomo_09_17_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 124.1 In SetExposureForMean 120, change by a factor of 0.9670 would require too small a change in exposure time Image shift -0.091 0.041 IS units -200.0 208.5 unbinned pixels; need stage -0.066 -0.077 if reset Tilting to -20 -20.00 degrees 4.1 1.1 unbinned pixels; 0.1 1.5 nm along two shift axes; -0.5 1.4 nm on specimen axes Rate = 7.55866 A/sec Drift is low enough after shot 1 Measured defocus = -3.85 microns changed by 0.10 to target drift = 0.08 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.265 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.08.09_batch_tomo_09_17_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 126.0 In SetExposureForMean 120, exposure time changed to 1.2000 Image shift -0.118 0.051 IS units -253.6 272.5 unbinned pixels; need stage -0.086 -0.105 if reset Tilting to -22 13.3 -8.8 unbinned pixels; 4.5 3.3 nm along two shift axes; 2.7 4.9 nm on specimen axes Rate = 27.89306 A/sec 7.1 1.6 unbinned pixels; 0.3 2.5 nm along two shift axes; -0.8 2.4 nm on specimen axes Rate = 12.81075 A/sec Drift is low enough after shot 2 Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.16 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.13 nm/sec Defocus = -0.215 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.09.12_batch_tomo_09_17_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 113.9 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift -0.050 -0.074 IS units 151.4 207.6 unbinned pixels; need stage -0.076 0.050 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees -2.3 1.2 unbinned pixels; 0.7 0.6 nm along two shift axes; -0.4 -0.9 nm on specimen axes Rate = 4.6274 A/sec Drift is low enough after shot 1 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.08 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -0.987 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.10.13_batch_tomo_09_17_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 123.1 In SetExposureForMean 120, change by a factor of 0.9749 would require too small a change in exposure time Image shift -0.131 0.097 IS units -392.8 265.0 unbinned pixels; need stage -0.079 -0.148 if reset Tilting to 4 Correcting tilt backlash 5.6 -0.2 unbinned pixels; 0.7 1.8 nm along two shift axes; -0.1 2.0 nm on specimen axes Rate = 9.8034 A/sec Drift is low enough after shot 1 Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.05 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -1.056 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.11.17_batch_tomo_09_17_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 122.2 In SetExposureForMean 120, change by a factor of 0.9817 would require too small a change in exposure time Image shift -0.119 0.082 IS units -340.4 246.3 unbinned pixels; need stage -0.074 -0.129 if reset Tilting to -24 -24.00 degrees -1.5 -1.7 unbinned pixels; 0.4 0.7 nm along two shift axes; 0.7 -0.5 nm on specimen axes Rate = 4.07853 A/sec Drift is low enough after shot 1 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.05 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -0.134 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.12.18_batch_tomo_09_17_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 124.7 In SetExposureForMean 120, change by a factor of 0.9629 would require too small a change in exposure time Image shift -0.057 -0.112 IS units 247.2 260.5 unbinned pixels; need stage -0.097 0.086 if reset Tilting to -26 11.9 -9.0 unbinned pixels; 4.4 2.9 nm along two shift axes; 2.8 4.4 nm on specimen axes Rate = 26.03649 A/sec 9.2 1.7 unbinned pixels; 0.5 3.2 nm along two shift axes; -0.9 3.2 nm on specimen axes Rate = 16.38932 A/sec 5.5 -0.2 unbinned pixels; 0.7 1.8 nm along two shift axes; -0.1 1.9 nm on specimen axes Rate = 9.60289 A/sec Drift is low enough after shot 3 Measured defocus = -3.81 microns changed by 0.06 to target drift = 0.15 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.19 nm/sec Defocus = -0.054 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.13.26_batch_tomo_09_17_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 123.9 In SetExposureForMean 120, change by a factor of 0.9697 would require too small a change in exposure time Image shift -0.013 -0.190 IS units 501.4 215.5 unbinned pixels; need stage -0.090 0.189 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees -9.2 3.5 unbinned pixels; 2.2 2.6 nm along two shift axes; -0.9 -3.3 nm on specimen axes Rate = 17.26367 A/sec -4.3 6.6 unbinned pixels; 2.7 0.6 nm along two shift axes; -2.2 -1.7 nm on specimen axes Rate = 13.94544 A/sec Drift is low enough after shot 2 Measured defocus = -3.69 microns changed by -0.06 to target drift = 0.14 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.15 nm/sec Defocus = -1.117 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.14.34_batch_tomo_09_17_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 121.2 In SetExposureForMean 120, change by a factor of 0.9904 would require too small a change in exposure time Image shift -0.173 0.158 IS units -597.1 319.9 unbinned pixels; need stage -0.092 -0.223 if reset Tilting to 8 Correcting tilt backlash 1.2 -2.9 unbinned pixels; 1.1 0.0 nm along two shift axes; 1.0 0.5 nm on specimen axes Rate = 5.48739 A/sec Drift is low enough after shot 1 Measured defocus = -3.64 microns changed by -0.11 to target drift = 0.11 nm/sec Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.08 nm/sec Defocus = -1.182 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.15.34_batch_tomo_09_17_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 120.1 In SetExposureForMean 120, change by a factor of 0.9990 would require too small a change in exposure time Image shift -0.141 0.104 IS units -420.3 284.1 unbinned pixels; need stage -0.085 -0.159 if reset Tilting to -28 -28.00 degrees 1.7 -3.5 unbinned pixels; 1.4 0.2 nm along two shift axes; 1.2 0.7 nm on specimen axes Rate = 6.92679 A/sec Drift is low enough after shot 1 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.14 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.14 nm/sec Defocus = 0.035 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.16.34_batch_tomo_09_17_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 123.0 In SetExposureForMean 120, change by a factor of 0.9768 would require too small a change in exposure time Image shift -0.008 -0.243 IS units 650.1 253.3 unbinned pixels; need stage -0.107 0.251 if reset Tilting to -30 13.8 -8.6 unbinned pixels; 4.5 3.5 nm along two shift axes; 2.6 5.1 nm on specimen axes Rate = 28.47737 A/sec 6.9 1.1 unbinned pixels; 0.4 2.4 nm along two shift axes; -0.6 2.4 nm on specimen axes Rate = 12.2446 A/sec Drift is low enough after shot 2 Measured defocus = -3.83 microns changed by 0.08 to target drift = 0.17 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.19 nm/sec Defocus = 0.136 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.17.37_batch_tomo_09_17_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 122.4 In SetExposureForMean 120, change by a factor of 0.9821 would require too small a change in exposure time Image shift 0.050 -0.341 IS units 974.2 187.9 unbinned pixels; need stage -0.095 0.391 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -21.6 6.0 unbinned pixels; 4.5 6.4 nm along two shift axes; -1.4 -7.8 nm on specimen axes Rate = 39.35322 A/sec -6.8 6.1 unbinned pixels; 2.8 1.5 nm along two shift axes; -1.9 -2.6 nm on specimen axes Rate = 16.11045 A/sec -6.5 2.6 unbinned pixels; 1.6 1.8 nm along two shift axes; -0.7 -2.4 nm on specimen axes Rate = 12.26804 A/sec Drift is low enough after shot 3 Measured defocus = -3.69 microns changed by -0.06 to target drift = 0.18 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.13 nm/sec Defocus = -1.249 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.18.52_batch_tomo_09_17_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 118.8 In SetExposureForMean 120, change by a factor of 1.0099 would require too small a change in exposure time Image shift -0.189 0.173 IS units -654.7 349.0 unbinned pixels; need stage -0.100 -0.247 if reset Tilting to 12 Correcting tilt backlash 6.0 -2.9 unbinned pixels; 1.7 1.6 nm along two shift axes; 0.8 2.2 nm on specimen axes Rate = 11.6581 A/sec Drift is low enough after shot 1 Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.24 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.18 nm/sec Defocus = -1.341 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.19.56_batch_tomo_09_17_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0220 would require too small a change in exposure time Image shift -0.147 0.088 IS units -383.1 315.5 unbinned pixels; need stage -0.097 -0.149 if reset Tilting to -32 -32.00 degrees 31.0 -3.4 unbinned pixels; 4.8 9.9 nm along two shift axes; 0.3 11.0 nm on specimen axes Rate = 54.70623 A/sec 4.1 0.5 unbinned pixels; 0.3 1.4 nm along two shift axes; -0.3 1.4 nm on specimen axes Rate = 7.31591 A/sec Drift is low enough after shot 2 Measured defocus = -3.84 microns changed by 0.09 to target drift = 0.19 nm/sec Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.12 nm/sec Defocus = 0.277 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.21.03_batch_tomo_09_17_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 121.8 In SetExposureForMean 120, change by a factor of 0.9870 would require too small a change in exposure time Image shift 0.056 -0.406 IS units 1155.2 233.4 unbinned pixels; need stage -0.116 0.473 if reset Tilting to -34 14.0 -9.0 unbinned pixels; 4.6 3.6 nm along two shift axes; 2.7 5.2 nm on specimen axes Rate = 29.12232 A/sec 13.7 0.2 unbinned pixels; 1.5 4.6 nm along two shift axes; -0.5 4.8 nm on specimen axes Rate = 24.08682 A/sec 6.9 -0.2 unbinned pixels; 0.9 2.3 nm along two shift axes; -0.1 2.4 nm on specimen axes Rate = 12.16458 A/sec Drift is low enough after shot 3 Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.21 nm/sec Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.27 nm/sec Defocus = 0.327 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.22.12_batch_tomo_09_17_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 120.3 In SetExposureForMean 120, change by a factor of 0.9995 would require too small a change in exposure time Image shift 0.120 -0.515 IS units 1514.8 162.7 unbinned pixels; need stage -0.103 0.640 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -12.7 1.9 unbinned pixels; 2.1 4.0 nm along two shift axes; -0.3 -4.5 nm on specimen axes Rate = 22.55345 A/sec -6.8 3.6 unbinned pixels; 2.0 1.8 nm along two shift axes; -1.0 -2.5 nm on specimen axes Rate = 13.47868 A/sec Drift is low enough after shot 2 Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.22 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.19 nm/sec Defocus = -1.339 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.23.20_batch_tomo_09_17_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, change by a factor of 1.0339 would require too small a change in exposure time Image shift -0.169 0.130 IS units -518.8 335.4 unbinned pixels; need stage -0.099 -0.200 if reset Tilting to 16 Correcting tilt backlash -8.5 1.2 unbinned pixels; 1.4 2.7 nm along two shift axes; -0.2 -3.0 nm on specimen axes Rate = 14.99972 A/sec -5.8 5.2 unbinned pixels; 2.4 1.3 nm along two shift axes; -1.6 -2.2 nm on specimen axes Rate = 13.66948 A/sec Drift is low enough after shot 2 Measured defocus = -3.69 microns changed by -0.06 to target drift = 0.22 nm/sec Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.22 nm/sec Defocus = -1.354 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.24.25_batch_tomo_09_17_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 114.6 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.152 0.090 IS units -394.5 329.2 unbinned pixels; need stage -0.101 -0.156 if reset Tilting to -36 -36.00 degrees 8.7 -4.1 unbinned pixels; 2.4 2.4 nm along two shift axes; 1.2 3.2 nm on specimen axes Rate = 16.80842 A/sec -0.7 1.1 unbinned pixels; 0.5 0.1 nm along two shift axes; -0.4 -0.3 nm on specimen axes Rate = 2.35697 A/sec Drift is low enough after shot 2 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.21 nm/sec Measured defocus = -3.84 microns changed by 0.09 to target drift = 0.19 nm/sec Defocus = 0.490 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.25.32_batch_tomo_09_17_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 130.1 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift 0.133 -0.582 IS units 1707.3 191.2 unbinned pixels; need stage -0.118 0.739 if reset Tilting to -38 18.9 -10.0 unbinned pixels; 5.5 5.1 nm along two shift axes; 2.9 6.9 nm on specimen axes Rate = 37.47139 A/sec 7.0 0.9 unbinned pixels; 0.5 2.4 nm along two shift axes; -0.5 2.4 nm on specimen axes Rate = 12.46694 A/sec Drift is low enough after shot 2 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.27 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.28 nm/sec Defocus = 0.464 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.26.34_batch_tomo_09_17_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 117.8 In SetExposureForMean 120, change by a factor of 1.0206 would require too small a change in exposure time Image shift 0.190 -0.685 IS units 2042.5 135.7 unbinned pixels; need stage -0.110 0.911 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -17.2 2.9 unbinned pixels; 3.0 5.3 nm along two shift axes; -0.5 -6.1 nm on specimen axes Rate = 30.597 A/sec -10.4 5.8 unbinned pixels; 3.1 2.7 nm along two shift axes; -1.7 -3.8 nm on specimen axes Rate = 20.81529 A/sec -9.8 3.7 unbinned pixels; 2.4 2.8 nm along two shift axes; -1.0 -3.5 nm on specimen axes Rate = 18.2844 A/sec -8.2 -3.1 unbinned pixels; 0.1 3.1 nm along two shift axes; 1.3 -2.8 nm on specimen axes Rate = 15.45371 A/sec -7.5 3.0 unbinned pixels; 1.9 2.1 nm along two shift axes; -0.8 -2.7 nm on specimen axes Rate = 14.16107 A/sec -7.5 3.7 unbinned pixels; 2.1 2.1 nm along two shift axes; -1.1 -2.8 nm on specimen axes Rate = 14.74717 A/sec -6.4 3.6 unbinned pixels; 1.9 1.7 nm along two shift axes; -1.1 -2.3 nm on specimen axes Rate = 12.828 A/sec Drift is low enough after shot 7 Measured defocus = -3.65 microns changed by -0.10 to target drift = 0.21 nm/sec Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.18 nm/sec Defocus = -1.422 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.28.12_batch_tomo_09_17_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 112.6 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.207 0.194 IS units -729.8 378.8 unbinned pixels; need stage -0.108 -0.284 if reset Tilting to 20 Correcting tilt backlash -12.5 1.5 unbinned pixels; 2.0 4.0 nm along two shift axes; -0.1 -4.4 nm on specimen axes Rate = 22.14691 A/sec -4.5 6.4 unbinned pixels; 2.6 0.7 nm along two shift axes; -2.1 -1.8 nm on specimen axes Rate = 13.70797 A/sec Drift is low enough after shot 2 Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.23 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.15 nm/sec Defocus = -1.415 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.29.17_batch_tomo_09_17_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 121.1 In SetExposureForMean 120, change by a factor of 0.9920 would require too small a change in exposure time Image shift -0.183 0.124 IS units -515.7 380.4 unbinned pixels; need stage -0.115 -0.207 if reset Tilting to -40 -40.00 degrees 12.2 -10.7 unbinned pixels; 5.0 2.7 nm along two shift axes; 3.4 4.6 nm on specimen axes Rate = 28.45385 A/sec 69.1 6.6 unbinned pixels; 5.9 23.7 nm along two shift axes; -4.4 24.0 nm on specimen axes Rate = 121.87967 A/sec -16.3 -3.1 unbinned pixels; 0.9 5.8 nm along two shift axes; 1.6 -5.6 nm on specimen axes Rate = 29.10387 A/sec 15.3 8.1 unbinned pixels; 0.9 6.0 nm along two shift axes; -3.3 5.1 nm on specimen axes Rate = 30.49015 A/sec 13.6 0.2 unbinned pixels; 1.5 4.5 nm along two shift axes; -0.5 4.8 nm on specimen axes Rate = 23.86697 A/sec -3.4 5.6 unbinned pixels; 2.2 0.5 nm along two shift axes; -1.9 -1.4 nm on specimen axes Rate = 11.47908 A/sec Drift is low enough after shot 6 Measured defocus = -3.91 microns changed by 0.16 to target drift = 0.13 nm/sec Measured defocus = -3.89 microns changed by 0.14 to target drift = 0.12 nm/sec Defocus = 0.764 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.30.48_batch_tomo_09_17_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 127.0 In SetExposureForMean 120, exposure time changed to 1.3200 Image shift 0.228 -0.808 IS units 2415.1 147.9 unbinned pixels; need stage -0.125 1.108 if reset Tilting to -42 21.7 -12.2 unbinned pixels; 6.6 5.7 nm along two shift axes; 3.6 8.0 nm on specimen axes Rate = 43.61839 A/sec 2.9 3.3 unbinned pixels; 0.8 1.4 nm along two shift axes; -1.3 0.9 nm on specimen axes Rate = 7.77411 A/sec Drift is low enough after shot 2 Measured defocus = -3.70 microns changed by -0.05 to target drift = 0.13 nm/sec Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.22 nm/sec Defocus = 0.756 um Dewars are NOT busy filling 11 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.31.51_batch_tomo_09_17_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 114.6 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.290 -0.918 IS units 2772.3 85.0 unbinned pixels; need stage -0.115 1.315 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -15.0 2.0 unbinned pixels; 2.4 4.7 nm along two shift axes; -0.2 -5.3 nm on specimen axes Rate = 26.53939 A/sec -2.9 7.0 unbinned pixels; 2.7 0.1 nm along two shift axes; -2.4 -1.2 nm on specimen axes Rate = 13.28929 A/sec Drift is low enough after shot 2 Measured defocus = -3.70 microns changed by -0.05 to target drift = 0.27 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.22 nm/sec Defocus = -1.477 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.33.01_batch_tomo_09_17_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 118.3 In SetExposureForMean 120, change by a factor of 1.0153 would require too small a change in exposure time Image shift -0.203 0.160 IS units -634.3 398.6 unbinned pixels; need stage -0.118 -0.256 if reset Tilting to 24 Correcting tilt backlash -15.2 -1.1 unbinned pixels; 1.4 5.2 nm along two shift axes; 0.9 -5.3 nm on specimen axes Rate = 26.83727 A/sec -7.9 5.8 unbinned pixels; 2.9 1.9 nm along two shift axes; -1.8 -3.0 nm on specimen axes Rate = 17.24606 A/sec -5.8 2.6 unbinned pixels; 1.5 1.6 nm along two shift axes; -0.7 -2.1 nm on specimen axes Rate = 11.12598 A/sec Drift is low enough after shot 3 Measured defocus = -3.86 microns changed by 0.11 to target drift = 0.30 nm/sec Measured defocus = -3.65 microns changed by -0.10 to target drift = 0.35 nm/sec Defocus = -1.464 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.34.11_batch_tomo_09_17_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, change by a factor of 1.0352 would require too small a change in exposure time Image shift -0.168 0.099 IS units -434.0 363.3 unbinned pixels; need stage -0.112 -0.181 if reset Tilting to -44 -44.00 degrees 38.0 -11.0 unbinned pixels; 8.1 11.2 nm along two shift axes; 2.7 13.6 nm on specimen axes Rate = 69.21928 A/sec -3.1 1.9 unbinned pixels; 1.0 0.8 nm along two shift axes; -0.6 -1.1 nm on specimen axes Rate = 6.32257 A/sec Drift is low enough after shot 2 Measured defocus = -3.83 microns changed by 0.08 to target drift = 0.26 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.28 nm/sec Defocus = 0.860 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.35.20_batch_tomo_09_17_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 123.2 In SetExposureForMean 120, change by a factor of 0.9760 would require too small a change in exposure time Image shift 0.333 -1.032 IS units 3124.6 76.9 unbinned pixels; need stage -0.123 1.532 if reset Tilting to -46 13.1 -9.2 unbinned pixels; 4.6 3.2 nm along two shift axes; 2.8 4.9 nm on specimen axes Rate = 28.06929 A/sec 8.0 1.0 unbinned pixels; 0.6 2.8 nm along two shift axes; -0.6 2.8 nm on specimen axes Rate = 14.12395 A/sec 2.5 0.9 unbinned pixels; 0.0 0.9 nm along two shift axes; -0.4 0.8 nm on specimen axes Rate = 4.63863 A/sec Drift is low enough after shot 3 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.19 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.29 nm/sec Defocus = 0.932 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.36.29_batch_tomo_09_17_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 120.8 In SetExposureForMean 120, change by a factor of 0.9953 would require too small a change in exposure time Image shift 0.384 -1.123 IS units 3420.5 22.8 unbinned pixels; need stage -0.113 1.740 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -24.1 3.6 unbinned pixels; 4.0 7.5 nm along two shift axes; -0.5 -8.5 nm on specimen axes Rate = 42.65626 A/sec -12.1 5.5 unbinned pixels; 3.2 3.3 nm along two shift axes; -1.5 -4.4 nm on specimen axes Rate = 23.21696 A/sec -9.0 4.2 unbinned pixels; 2.4 2.5 nm along two shift axes; -1.2 -3.3 nm on specimen axes Rate = 17.33527 A/sec -15.0 1.3 unbinned pixels; 2.2 4.8 nm along two shift axes; 0.0 -5.3 nm on specimen axes Rate = 26.37824 A/sec -8.6 2.1 unbinned pixels; 1.7 2.6 nm along two shift axes; -0.5 -3.1 nm on specimen axes Rate = 15.56416 A/sec -6.6 1.4 unbinned pixels; 1.2 2.0 nm along two shift axes; -0.3 -2.4 nm on specimen axes Rate = 11.88387 A/sec Drift is low enough after shot 6 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.19 nm/sec Measured defocus = -3.67 microns changed by -0.08 to target drift = 0.30 nm/sec Defocus = -1.576 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.38.02_batch_tomo_09_17_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 112.9 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift -0.190 0.129 IS units -537.7 395.6 unbinned pixels; need stage -0.120 -0.226 if reset Tilting to 28 Correcting tilt backlash -15.9 2.5 unbinned pixels; 2.7 5.0 nm along two shift axes; -0.4 -5.7 nm on specimen axes Rate = 28.27523 A/sec -7.3 3.7 unbinned pixels; 2.1 2.0 nm along two shift axes; -1.1 -2.7 nm on specimen axes Rate = 14.25503 A/sec -8.5 1.9 unbinned pixels; 1.6 2.6 nm along two shift axes; -0.4 -3.0 nm on specimen axes Rate = 15.29738 A/sec -7.9 1.5 unbinned pixels; 1.4 2.4 nm along two shift axes; -0.3 -2.8 nm on specimen axes Rate = 14.09052 A/sec -8.0 1.2 unbinned pixels; 1.3 2.5 nm along two shift axes; -0.2 -2.8 nm on specimen axes Rate = 14.21326 A/sec -6.1 1.1 unbinned pixels; 1.1 1.9 nm along two shift axes; -0.2 -2.2 nm on specimen axes Rate = 10.88813 A/sec Drift is low enough after shot 6 Measured defocus = -3.89 microns changed by 0.14 to target drift = 0.38 nm/sec Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.29 nm/sec Defocus = -1.472 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.39.30_batch_tomo_09_17_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0086 would require too small a change in exposure time Image shift -0.174 0.065 IS units -346.7 411.1 unbinned pixels; need stage -0.131 -0.154 if reset Tilting to -48 -48.00 degrees -7.8 -2.8 unbinned pixels; 0.0 2.9 nm along two shift axes; 1.2 -2.7 nm on specimen axes Rate = 14.66472 A/sec 9.7 0.3 unbinned pixels; 1.0 3.2 nm along two shift axes; -0.4 3.4 nm on specimen axes Rate = 16.97423 A/sec 51.2 -6.2 unbinned pixels; 8.0 16.2 nm along two shift axes; 0.6 18.1 nm on specimen axes Rate = 90.36718 A/sec 5.7 0.2 unbinned pixels; 0.6 1.9 nm along two shift axes; -0.2 2.0 nm on specimen axes Rate = 10.05305 A/sec Drift is low enough after shot 4 Measured defocus = -3.89 microns changed by 0.14 to target drift = 0.35 nm/sec Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.27 nm/sec Defocus = 1.067 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.40.50_batch_tomo_09_17_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 128.6 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift 0.419 -1.211 IS units 3693.7 11.6 unbinned pixels; need stage -0.118 1.951 if reset Tilting to -50 12.2 -7.5 unbinned pixels; 3.9 3.2 nm along two shift axes; 2.2 4.5 nm on specimen axes Rate = 25.10705 A/sec 6.3 1.6 unbinned pixels; 0.2 2.3 nm along two shift axes; -0.8 2.2 nm on specimen axes Rate = 11.52015 A/sec Drift is low enough after shot 2 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.48 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.22 nm/sec Defocus = 1.140 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.41.54_batch_tomo_09_17_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 116.0 In SetExposureForMean 120, change by a factor of 1.0372 would require too small a change in exposure time Image shift 0.458 -1.292 IS units 3951.5 -16.8 unbinned pixels; need stage -0.116 2.174 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -28.3 7.1 unbinned pixels; 5.6 8.5 nm along two shift axes; -1.6 -10.1 nm on specimen axes Rate = 51.062 A/sec -12.8 5.5 unbinned pixels; 3.3 3.6 nm along two shift axes; -1.5 -4.7 nm on specimen axes Rate = 24.50382 A/sec -10.0 4.5 unbinned pixels; 2.6 2.8 nm along two shift axes; -1.3 -3.6 nm on specimen axes Rate = 19.18388 A/sec -9.7 -1.5 unbinned pixels; 0.6 3.4 nm along two shift axes; 0.8 -3.4 nm on specimen axes Rate = 17.25918 A/sec -11.6 4.9 unbinned pixels; 3.0 3.3 nm along two shift axes; -1.4 -4.2 nm on specimen axes Rate = 22.11991 A/sec -7.3 2.9 unbinned pixels; 1.8 2.1 nm along two shift axes; -0.8 -2.7 nm on specimen axes Rate = 13.81689 A/sec Drift is low enough after shot 6 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.31 nm/sec Measured defocus = -3.83 microns changed by 0.08 to target drift = 0.16 nm/sec Defocus = -1.422 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.43.29_batch_tomo_09_17_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 106.8 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift -0.185 0.095 IS units -440.4 411.7 unbinned pixels; need stage -0.128 -0.195 if reset Tilting to 32 Correcting tilt backlash -22.4 0.8 unbinned pixels; 2.9 7.3 nm along two shift axes; 0.4 -7.9 nm on specimen axes Rate = 39.32455 A/sec -4.8 2.8 unbinned pixels; 1.5 1.2 nm along two shift axes; -0.8 -1.8 nm on specimen axes Rate = 9.70646 A/sec Drift is low enough after shot 2 Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.37 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.34 nm/sec Defocus = -1.461 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.44.35_batch_tomo_09_17_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 121.1 In SetExposureForMean 120, change by a factor of 0.9942 would require too small a change in exposure time Image shift -0.159 0.023 IS units -218.7 411.3 unbinned pixels; need stage -0.135 -0.107 if reset Tilting to -52 -52.00 degrees 6.2 -3.7 unbinned pixels; 2.0 1.6 nm along two shift axes; 1.1 2.3 nm on specimen axes Rate = 12.70001 A/sec Drift is low enough after shot 1 Measured defocus = -3.98 microns changed by 0.23 to target drift = 0.40 nm/sec Measured defocus = -3.67 microns changed by -0.08 to target drift = 0.26 nm/sec Defocus = 1.288 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.45.38_batch_tomo_09_17_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 132.5 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.510 -1.393 IS units 4275.3 -62.2 unbinned pixels; need stage -0.110 2.459 if reset Tilting to -54 7.5 -6.2 unbinned pixels; 2.9 1.8 nm along two shift axes; 1.9 2.8 nm on specimen axes Rate = 17.07688 A/sec 8.6 3.2 unbinned pixels; 0.0 3.2 nm along two shift axes; -1.4 2.9 nm on specimen axes Rate = 16.11453 A/sec 8.4 0.1 unbinned pixels; 1.0 2.8 nm along two shift axes; -0.3 2.9 nm on specimen axes Rate = 14.70497 A/sec 7.7 -0.3 unbinned pixels; 1.0 2.5 nm along two shift axes; -0.1 2.7 nm on specimen axes Rate = 13.43487 A/sec Drift is low enough after shot 4 Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.22 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.36 nm/sec Defocus = 1.357 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.46.53_batch_tomo_09_17_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 119.7 In SetExposureForMean 120, change by a factor of 1.0036 would require too small a change in exposure time Image shift 0.536 -1.453 IS units 4464.1 -75.9 unbinned pixels; need stage -0.111 2.690 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -29.4 6.1 unbinned pixels; 5.5 9.0 nm along two shift axes; -1.3 -10.5 nm on specimen axes Rate = 52.57954 A/sec -13.8 5.1 unbinned pixels; 3.3 4.0 nm along two shift axes; -1.4 -5.0 nm on specimen axes Rate = 25.80658 A/sec -8.7 4.1 unbinned pixels; 2.4 2.4 nm along two shift axes; -1.2 -3.2 nm on specimen axes Rate = 16.83382 A/sec -1.1 0.2 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.0 -0.4 nm on specimen axes Rate = 1.97309 A/sec Drift is low enough after shot 4 Measured defocus = -4.35 microns changed by 0.60 to target drift = 1.07 nm/sec Measured defocus = -3.02 microns changed by -0.73 to target drift = 0.53 nm/sec Defocus = -1.597 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.48.15_batch_tomo_09_17_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 108.5 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift -0.141 0.016 IS units -181.1 368.4 unbinned pixels; need stage -0.121 -0.092 if reset Tilting to 36 Correcting tilt backlash Trimming A by 25 25, reference by 20 20 -0.1 -1.4 unbinned pixels; 0.5 0.2 nm along two shift axes; 0.5 -0.0 nm on specimen axes Rate = 2.49199 A/sec Drift is low enough after shot 1 Measured defocus = -3.52 microns changed by -0.23 to target drift = 0.08 nm/sec Measured defocus = -4.36 microns changed by 0.61 to target drift = 0.07 nm/sec Defocus = -1.210 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.49.15_batch_tomo_09_17_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 113.3 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift -0.123 -0.040 IS units -12.0 372.9 unbinned pixels; need stage -0.128 -0.020 if reset Tilting to -56 -56.00 degrees 13.8 -8.6 unbinned pixels; 4.5 3.5 nm along two shift axes; 2.6 5.1 nm on specimen axes Rate = 28.43034 A/sec 15.2 2.9 unbinned pixels; 0.8 5.4 nm along two shift axes; -1.5 5.2 nm on specimen axes Rate = 27.23034 A/sec -18.4 4.1 unbinned pixels; 3.5 5.6 nm along two shift axes; -0.9 -6.6 nm on specimen axes Rate = 33.10516 A/sec -6.9 1.4 unbinned pixels; 1.3 2.1 nm along two shift axes; -0.3 -2.5 nm on specimen axes Rate = 12.4137 A/sec Drift is low enough after shot 4 Measured defocus = -3.94 microns changed by 0.19 to target drift = 0.18 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.12 nm/sec Defocus = 1.524 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.50.41_batch_tomo_09_17_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 133.8 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.544 -1.469 IS units 4515.4 -84.4 unbinned pixels; need stage -0.110 2.860 if reset Tilting to -58 3.1 -4.7 unbinned pixels; 1.9 0.4 nm along two shift axes; 1.6 1.2 nm on specimen axes Rate = 9.91089 A/sec Drift is low enough after shot 1 Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.15 nm/sec Measured defocus = -3.70 microns changed by -0.05 to target drift = 0.10 nm/sec Defocus = 1.524 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.51.39_batch_tomo_09_17_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 112.3 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.582 -1.540 IS units 4745.4 -118.3 unbinned pixels; need stage -0.105 3.174 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -12.8 5.3 unbinned pixels; 3.3 3.6 nm along two shift axes; -1.5 -4.6 nm on specimen axes Rate = 24.26071 A/sec Trimming A by 20 25, reference by 20 20 -0.8 5.1 unbinned pixels; 1.8 0.4 nm along two shift axes; -1.8 -0.4 nm on specimen axes Rate = 9.17649 A/sec Drift is low enough after shot 2 Measured defocus = -3.30 microns changed by -0.45 to target drift = 0.60 nm/sec Measured defocus = -3.63 microns changed by -0.12 to target drift = 0.43 nm/sec Defocus = -1.778 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.52.51_batch_tomo_09_17_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 108.7 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift -0.120 -0.029 IS units -40.1 354.4 unbinned pixels; need stage -0.121 -0.033 if reset Tilting to 40 Correcting tilt backlash Trimming A by 40 40, reference by 20 20 Trimming A by 25 25, reference by 20 20 -7.6 -0.5 unbinned pixels; 0.7 2.6 nm along two shift axes; 0.4 -2.7 nm on specimen axes Rate = 13.38965 A/sec Drift is low enough after shot 1 Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.15 nm/sec Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.19 nm/sec Defocus = -1.780 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.53.51_batch_tomo_09_17_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 118.0 In SetExposureForMean 120, change by a factor of 1.0230 would require too small a change in exposure time Image shift -0.092 -0.097 IS units 171.8 343.5 unbinned pixels; need stage -0.123 0.065 if reset Tilting to -60 -60.00 degrees 16.2 -6.8 unbinned pixels; 4.1 4.6 nm along two shift axes; 1.9 5.9 nm on specimen axes Rate = 30.7943 A/sec 5.8 1.5 unbinned pixels; 0.2 2.1 nm along two shift axes; -0.7 2.0 nm on specimen axes Rate = 10.57381 A/sec Drift is low enough after shot 2 Measured defocus = -3.87 microns changed by 0.12 to target drift = 0.16 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.33 nm/sec Defocus = 1.647 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.55.02_batch_tomo_09_17_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 133.4 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.613 -1.621 IS units 4995.2 -127.6 unbinned pixels; need stage -0.110 3.541 if reset Tilting to -62 10.1 -6.2 unbinned pixels; 3.2 2.6 nm along two shift axes; 1.9 3.7 nm on specimen axes Rate = 20.70831 A/sec 1.5 2.7 unbinned pixels; 0.7 0.8 nm along two shift axes; -1.0 0.5 nm on specimen axes Rate = 5.53319 A/sec Drift is low enough after shot 2 Measured defocus = -3.67 microns changed by -0.08 to target drift = 0.13 nm/sec Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.17 nm/sec Defocus = 1.629 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.56.05_batch_tomo_09_17_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 112.1 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.650 -1.685 IS units 5204.7 -165.7 unbinned pixels; need stage -0.103 3.931 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees Trimming A by 35 35, reference by 20 25 Trimming A by 25 30, reference by 20 25 -21.0 -0.5 unbinned pixels; 2.3 7.0 nm along two shift axes; 0.8 -7.4 nm on specimen axes Rate = 36.93105 A/sec Trimming A by 30 30, reference by 20 25 -2.4 3.0 unbinned pixels; 1.3 0.4 nm along two shift axes; -1.0 -0.9 nm on specimen axes Rate = 6.76505 A/sec Drift is low enough after shot 2 Measured defocus = -3.65 microns changed by -0.10 to target drift = 0.58 nm/sec Measured defocus = -3.63 microns changed by -0.12 to target drift = 0.49 nm/sec Defocus = -2.004 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.57.18_batch_tomo_09_17_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 104.0 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift -0.060 -0.138 IS units 315.1 295.6 unbinned pixels; need stage -0.111 0.137 if reset Tilting to 44 Correcting tilt backlash Trimming A by 35 35, reference by 20 20 Trimming A by 25 25, reference by 20 20 -35.4 5.3 unbinned pixels; 5.9 11.1 nm along two shift axes; -0.8 -12.6 nm on specimen axes Rate = 62.83053 A/sec Trimming A by 25 25, reference by 20 20 -24.3 4.2 unbinned pixels; 4.2 7.5 nm along two shift axes; -0.7 -8.6 nm on specimen axes Rate = 43.20726 A/sec Trimming A by 25 25, reference by 20 20 -16.8 3.4 unbinned pixels; 3.1 5.2 nm along two shift axes; -0.7 -6.0 nm on specimen axes Rate = 30.01587 A/sec Trimming A by 25 30, reference by 20 20 -10.9 1.9 unbinned pixels; 1.9 3.4 nm along two shift axes; -0.3 -3.9 nm on specimen axes Rate = 19.46379 A/sec Trimming A by 25 30, reference by 20 20 -10.2 2.0 unbinned pixels; 1.9 3.1 nm along two shift axes; -0.4 -3.6 nm on specimen axes Rate = 18.26254 A/sec Trimming A by 25 30, reference by 20 20 -9.8 1.5 unbinned pixels; 1.6 3.1 nm along two shift axes; -0.2 -3.5 nm on specimen axes Rate = 17.3507 A/sec Trimming A by 30 30, reference by 20 20 21.7 1.4 unbinned pixels; 2.1 7.4 nm along two shift axes; -1.2 7.6 nm on specimen axes Rate = 38.24717 A/sec Trimming A by 30 30, reference by 20 20 38.3 -5.9 unbinned pixels; 6.4 12.0 nm along two shift axes; 0.9 13.6 nm on specimen axes Rate = 67.98324 A/sec Trimming A by 30 30, reference by 20 20 23.7 -4.3 unbinned pixels; 4.2 7.3 nm along two shift axes; 0.8 8.4 nm on specimen axes Rate = 42.20246 A/sec Trimming A by 30 30, reference by 20 20 17.3 -3.6 unbinned pixels; 3.2 5.3 nm along two shift axes; 0.7 6.2 nm on specimen axes Rate = 30.99084 A/sec Drift never got below 14: Skipping ... Measured defocus = -3.83 microns changed by 0.08 to target drift = 0.64 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.43 nm/sec Defocus = -1.935 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_02.59.10_batch_tomo_09_17_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 110.7 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift -0.022 -0.210 IS units 546.3 259.2 unbinned pixels; need stage -0.106 0.257 if reset Tilting to -64 -64.00 degrees 1.2 -5.9 unbinned pixels; 2.1 0.3 nm along two shift axes; 2.0 0.6 nm on specimen axes Rate = 10.52547 A/sec Drift is low enough after shot 1 Measured defocus = -3.90 microns changed by 0.15 to target drift = 0.14 nm/sec Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.18 nm/sec Defocus = 1.806 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.00.15_batch_tomo_09_17_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 145.1 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift 0.667 -1.715 IS units 5304.5 -183.7 unbinned pixels; need stage -0.100 4.292 if reset Tilting to -66 5.3 -5.6 unbinned pixels; 2.5 1.1 nm along two shift axes; 1.8 2.0 nm on specimen axes Rate = 13.5026 A/sec Drift is low enough after shot 1 Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.17 nm/sec Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.15 nm/sec Defocus = 1.753 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.01.14_batch_tomo_09_17_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.695 -1.760 IS units 5451.4 -217.2 unbinned pixels; need stage -0.093 4.756 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees Trimming A by 45 45, reference by 25 25 Trimming A by 30 35, reference by 25 25 -9.3 2.4 unbinned pixels; 1.9 2.8 nm along two shift axes; -0.6 -3.3 nm on specimen axes Rate = 16.81398 A/sec Trimming A by 35 35, reference by 25 25 0.1 4.2 unbinned pixels; 1.4 0.5 nm along two shift axes; -1.5 -0.1 nm on specimen axes Rate = 7.46605 A/sec Drift is low enough after shot 2 Measured defocus = -3.64 microns changed by -0.11 to target drift = 0.16 nm/sec Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.15 nm/sec Defocus = -2.070 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.02.26_batch_tomo_09_17_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 104.7 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.005 -0.265 IS units 722.2 238.8 unbinned pixels; need stage -0.104 0.356 if reset Tilting to 48 Correcting tilt backlash Trimming A by 55 55, reference by 35 35 Trimming A by 45 45, reference by 35 35 -3.1 1.5 unbinned pixels; 0.9 0.8 nm along two shift axes; -0.4 -1.1 nm on specimen axes Rate = 6.06566 A/sec Drift is low enough after shot 1 Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.10 nm/sec Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.17 nm/sec Defocus = -2.106 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.03.27_batch_tomo_09_17_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 113.3 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.032 -0.324 IS units 908.5 221.5 unbinned pixels; need stage -0.104 0.469 if reset Tilting to -68 -68.00 degrees 10.8 -5.8 unbinned pixels; 3.2 2.9 nm along two shift axes; 1.7 4.0 nm on specimen axes Rate = 21.53096 A/sec 0.4 2.8 unbinned pixels; 0.9 0.5 nm along two shift axes; -1.0 0.0 nm on specimen axes Rate = 4.98671 A/sec Drift is low enough after shot 2 Measured defocus = -3.93 microns changed by 0.18 to target drift = 0.10 nm/sec Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.14 nm/sec Defocus = 1.865 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.04.38_batch_tomo_09_17_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 150.4 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.746 -1.834 IS units 5702.1 -285.4 unbinned pixels; need stage -0.077 5.407 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = -0.19 microns changed by -3.56 to target drift = 0.38 nm/sec Focus area changed from stored state, axis position -2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_10.st Running script 1 at item 19 (-385.15, -438.96) 03:05:35 Refine ZLP: additional offset = -0.3 eV; total offset = -14.4 eV Item 19: Stage: -385.15 -438.96 -223 Label: 19 Note: Sec 18 - anchor_maps.map Target defocus is: -3.75 um Target defocus is: -4.00 um Target defocus = -4 Trimming A by 25 130, reference by 30 130 Angle = -4.40, increment = 0.60, shift = 0.6 -6.2 pixels, -0.000 um Y Trimming A by 21 130, reference by 30 130 Angle = -3.20, increment = 1.80, shift = -7.5 -7.8 pixels, -0.019 um Y Trimming A by 21 130, reference by 30 130 Angle = 0.41, increment = 5.41, shift = -26.4 -15.4 pixels, -0.064 um Y Trimming A by 21 130, reference by 30 130 Angle = 3.00, increment = 8.00, shift = -21.3 -17.7 pixels, -0.053 um Y Rough eucentricity: changing Z by 0.38 to -223.03, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 24 135, reference by 24 135 Angle = 18.00, shift = -21.6, 4.6 pixels, 0.016, -0.048 um, IS = -0.016, 0.048 Trimming A by 24 135, reference by 24 135 Angle = 11.00, shift = -33.8, -2.8 pixels, 0.002, -0.077 um, IS = -0.018, 0.125 Trimming A by 34 130, reference by 34 130 Angle = 4.00, shift = 4.9, 5.4 pixels, 0.011, 0.012 um, IS = -0.029, 0.113 Trimming A by 21 20 20 130, reference by 21 23 20 135 Angle = -4.00, shift = -15.9, -19.4 pixels, -0.040, -0.041 um, IS = 0.011, 0.154 Trimming A by 20 130, reference by 21 130 Angle = -11.00, shift = 14.1, -6.5 pixels, -0.018, 0.030 um, IS = 0.029, 0.123 Trimming A by 34 130, reference by 25 130 Angle = -18.00, shift = -22.2, 2.0 pixels, 0.010, -0.050 um, IS = 0.019, 0.173 Trimming A by 34 130, reference by 34 130 Angle = -24.00, shift = -5.8, 3.7 pixels, 0.010, -0.012 um, IS = 0.009, 0.185 General solution delZ 0.19, delY 0.34, yZero 0.13 solution with assumed YZero 0.13: delZ 0.19, delY 0.33 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.19 microns and lateral displacement of -0.34 microns Trimming A by 21 21 20 140, reference by 21 21 20 140 Disparity in stage position after aligning to center of map frame: 0.01 -0.08 Aligning to target with image shift equivalent to stage shift: 0.01 -0.07 Disparity in stage position after aligning to map item itself: 0.00 -0.07 (align shift -0.01 -0.02) Finished aligning to item in 2 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -385.14 -438.94 -223.21 0.7 -0.1 unbinned pixels; 0.1 0.2 nm along two shift axes; -0.0 0.2 nm on specimen axes Rate = 1.19128 A/sec Drift is low enough after shot 1 Measured defocus = 1.21 microns changed by -5.21 to target drift = 0.02 nm/sec Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.03 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -1.261 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.10.27_batch_tomo_10_19_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 101.2 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.042 0.083 IS units -265.9 35.7 unbinned pixels; need stage -0.004 -0.097 if reset Tilting to -8 Correcting tilt backlash 10.7 -0.2 unbinned pixels; 1.3 3.5 nm along two shift axes; -0.3 3.8 nm on specimen axes Rate = 18.74468 A/sec 5.2 0.4 unbinned pixels; 0.5 1.8 nm along two shift axes; -0.3 1.8 nm on specimen axes Rate = 9.11767 A/sec Drift is low enough after shot 2 Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.08 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -1.322 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.11.32_batch_tomo_10_19_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 121.3 In SetExposureForMean 120, change by a factor of 0.9894 would require too small a change in exposure time Image shift -0.071 0.085 IS units -299.8 114.0 unbinned pixels; need stage -0.030 -0.111 if reset Tilting to -12 -12.00 degrees -7.8 1.6 unbinned pixels; 1.4 2.4 nm along two shift axes; -0.3 -2.8 nm on specimen axes Rate = 13.8749 A/sec Drift is low enough after shot 1 Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.09 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.220 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.12.31_batch_tomo_10_19_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 121.3 In SetExposureForMean 120, change by a factor of 0.9892 would require too small a change in exposure time Image shift -0.103 0.139 IS units -478.6 147.8 unbinned pixels; need stage -0.036 -0.178 if reset Tilting to -14 4.3 -0.9 unbinned pixels; 0.8 1.3 nm along two shift axes; 0.2 1.6 nm on specimen axes Rate = 7.78097 A/sec Drift is low enough after shot 1 Measured defocus = -4.08 microns changed by 0.08 to target drift = 0.12 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -1.126 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.13.28_batch_tomo_10_19_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 121.0 In SetExposureForMean 120, change by a factor of 0.9922 would require too small a change in exposure time Image shift -0.139 0.216 IS units -721.5 173.4 unbinned pixels; need stage -0.037 -0.269 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 12.2 -0.6 unbinned pixels; 1.6 4.0 nm along two shift axes; -0.2 4.3 nm on specimen axes Rate = 21.47782 A/sec 6.4 -0.5 unbinned pixels; 0.9 2.1 nm along two shift axes; -0.0 2.3 nm on specimen axes Rate = 11.2315 A/sec Drift is low enough after shot 2 Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.388 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.14.34_batch_tomo_10_19_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 121.2 In SetExposureForMean 120, change by a factor of 0.9904 would require too small a change in exposure time Image shift -0.073 0.084 IS units -299.3 119.3 unbinned pixels; need stage -0.032 -0.110 if reset Tilting to -4 Correcting tilt backlash -6.8 0.3 unbinned pixels; 0.9 2.2 nm along two shift axes; 0.1 -2.4 nm on specimen axes Rate = 11.86657 A/sec Drift is low enough after shot 1 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.12 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -1.471 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.15.33_batch_tomo_10_19_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 120.8 In SetExposureForMean 120, change by a factor of 0.9930 would require too small a change in exposure time Image shift -0.103 0.145 IS units -495.4 141.6 unbinned pixels; need stage -0.033 -0.180 if reset Tilting to -16 -16.00 degrees -0.7 -6.5 unbinned pixels; 2.1 1.0 nm along two shift axes; 2.3 -0.1 nm on specimen axes Rate = 11.56308 A/sec Drift is low enough after shot 1 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.03 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.00 nm/sec Defocus = -1.062 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.16.32_batch_tomo_10_19_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 120.7 In SetExposureForMean 120, change by a factor of 0.9942 would require too small a change in exposure time Image shift -0.115 0.143 IS units -502.3 177.2 unbinned pixels; need stage -0.046 -0.191 if reset Tilting to -18 -2.6 -0.3 unbinned pixels; 0.2 0.9 nm along two shift axes; 0.2 -0.9 nm on specimen axes Rate = 4.63649 A/sec Drift is low enough after shot 1 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.02 nm/sec Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.02 nm/sec Defocus = -1.016 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.17.29_batch_tomo_10_19_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 120.3 In SetExposureForMean 120, change by a factor of 0.9978 would require too small a change in exposure time Image shift -0.151 0.233 IS units -779.9 190.3 unbinned pixels; need stage -0.041 -0.296 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 13.1 -1.1 unbinned pixels; 1.9 4.2 nm along two shift axes; -0.0 4.6 nm on specimen axes Rate = 23.01034 A/sec 8.5 1.4 unbinned pixels; 0.5 3.0 nm along two shift axes; -0.8 2.9 nm on specimen axes Rate = 15.19202 A/sec 4.6 -0.1 unbinned pixels; 0.6 1.5 nm along two shift axes; -0.1 1.6 nm on specimen axes Rate = 8.14833 A/sec Drift is low enough after shot 3 Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.07 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.545 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.18.42_batch_tomo_10_19_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 120.3 In SetExposureForMean 120, change by a factor of 0.9976 would require too small a change in exposure time Image shift -0.125 0.191 IS units -639.9 158.9 unbinned pixels; need stage -0.035 -0.232 if reset Tilting to 0 Correcting tilt backlash -7.2 -4.4 unbinned pixels; 0.6 2.9 nm along two shift axes; 1.8 -2.4 nm on specimen axes Rate = 14.9569 A/sec -3.1 2.2 unbinned pixels; 1.1 0.7 nm along two shift axes; -0.7 -1.1 nm on specimen axes Rate = 6.63377 A/sec Drift is low enough after shot 2 Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.11 nm/sec Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.05 nm/sec Defocus = -1.651 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.19.48_batch_tomo_10_19_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 119.8 In SetExposureForMean 120, change by a factor of 1.0018 would require too small a change in exposure time Image shift -0.090 0.140 IS units -467.2 112.1 unbinned pixels; need stage -0.024 -0.169 if reset Tilting to -20 -20.00 degrees -2.9 -1.5 unbinned pixels; 0.2 1.1 nm along two shift axes; 0.6 -1.0 nm on specimen axes Rate = 5.75227 A/sec Drift is low enough after shot 1 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.00 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.01 nm/sec Defocus = -0.946 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.20.48_batch_tomo_10_19_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 119.7 In SetExposureForMean 120, change by a factor of 1.0030 would require too small a change in exposure time Image shift -0.141 0.193 IS units -662.5 200.1 unbinned pixels; need stage -0.048 -0.256 if reset Tilting to -22 9.0 -2.1 unbinned pixels; 1.7 2.7 nm along two shift axes; 0.5 3.2 nm on specimen axes Rate = 16.14516 A/sec 6.7 -1.0 unbinned pixels; 1.1 2.1 nm along two shift axes; 0.2 2.4 nm on specimen axes Rate = 11.96427 A/sec Drift is low enough after shot 2 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.09 nm/sec Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.08 nm/sec Defocus = -0.902 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.21.52_batch_tomo_10_19_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 118.8 In SetExposureForMean 120, change by a factor of 1.0108 would require too small a change in exposure time Image shift -0.124 0.176 IS units -598.9 170.8 unbinned pixels; need stage -0.040 -0.234 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 9.3 0.7 unbinned pixels; 0.8 3.2 nm along two shift axes; -0.5 3.2 nm on specimen axes Rate = 16.33219 A/sec 3.6 0.6 unbinned pixels; 0.2 1.3 nm along two shift axes; -0.3 1.3 nm on specimen axes Rate = 6.4632 A/sec Drift is low enough after shot 2 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.07 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.714 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.23.00_batch_tomo_10_19_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 119.1 In SetExposureForMean 120, change by a factor of 1.0077 would require too small a change in exposure time Image shift -0.099 0.138 IS units -470.0 138.0 unbinned pixels; need stage -0.033 -0.171 if reset Tilting to 4 Correcting tilt backlash -31.5 5.5 unbinned pixels; 5.5 9.8 nm along two shift axes; -1.0 -11.2 nm on specimen axes Rate = 56.03771 A/sec -18.6 7.0 unbinned pixels; 4.5 5.3 nm along two shift axes; -1.9 -6.7 nm on specimen axes Rate = 34.7144 A/sec -15.6 4.8 unbinned pixels; 3.4 4.6 nm along two shift axes; -1.2 -5.6 nm on specimen axes Rate = 28.56913 A/sec -9.9 1.8 unbinned pixels; 1.8 3.1 nm along two shift axes; -0.3 -3.5 nm on specimen axes Rate = 17.66607 A/sec -13.2 2.3 unbinned pixels; 2.3 4.1 nm along two shift axes; -0.4 -4.7 nm on specimen axes Rate = 23.52358 A/sec -6.5 2.3 unbinned pixels; 1.5 1.9 nm along two shift axes; -0.6 -2.3 nm on specimen axes Rate = 12.04052 A/sec Drift is low enough after shot 6 Measured defocus = -3.89 microns changed by -0.11 to target drift = 0.20 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.17 nm/sec Defocus = -1.811 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.24.28_batch_tomo_10_19_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 118.4 In SetExposureForMean 120, change by a factor of 1.0136 would require too small a change in exposure time Image shift -0.158 0.244 IS units -816.0 198.0 unbinned pixels; need stage -0.043 -0.296 if reset Tilting to -24 -24.00 degrees 1.5 1.4 unbinned pixels; 0.3 0.7 nm along two shift axes; -0.5 0.5 nm on specimen axes Rate = 3.61073 A/sec Drift is low enough after shot 1 Measured defocus = -4.15 microns changed by 0.15 to target drift = 0.00 nm/sec Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -0.748 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.25.28_batch_tomo_10_19_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 118.2 In SetExposureForMean 120, change by a factor of 1.0157 would require too small a change in exposure time Image shift -0.148 0.193 IS units -670.4 220.0 unbinned pixels; need stage -0.055 -0.267 if reset Tilting to -26 6.7 -7.5 unbinned pixels; 3.3 1.3 nm along two shift axes; 2.4 2.6 nm on specimen axes Rate = 17.58989 A/sec 7.8 2.3 unbinned pixels; 0.1 2.9 nm along two shift axes; -1.1 2.7 nm on specimen axes Rate = 14.30749 A/sec 3.4 1.1 unbinned pixels; 0.0 1.3 nm along two shift axes; -0.5 1.2 nm on specimen axes Rate = 6.38807 A/sec Drift is low enough after shot 3 Measured defocus = -4.06 microns changed by 0.06 to target drift = 0.06 nm/sec Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.09 nm/sec Defocus = -0.712 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.26.37_batch_tomo_10_19_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0232 would require too small a change in exposure time Image shift -0.107 0.128 IS units -453.2 170.4 unbinned pixels; need stage -0.045 -0.184 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 0.3 0.9 unbinned pixels; 0.3 0.2 nm along two shift axes; -0.3 0.1 nm on specimen axes Rate = 1.6663 A/sec Drift is low enough after shot 1 Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.11 nm/sec Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.07 nm/sec Defocus = -1.960 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.27.40_batch_tomo_10_19_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0220 would require too small a change in exposure time Image shift -0.142 0.211 IS units -711.4 186.5 unbinned pixels; need stage -0.042 -0.259 if reset Tilting to 8 Correcting tilt backlash 3.3 -3.2 unbinned pixels; 1.5 0.7 nm along two shift axes; 1.0 1.3 nm on specimen axes Rate = 8.12884 A/sec Drift is low enough after shot 1 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.10 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -2.020 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.28.39_batch_tomo_10_19_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 116.6 In SetExposureForMean 120, change by a factor of 1.0295 would require too small a change in exposure time Image shift -0.103 0.146 IS units -497.0 141.5 unbinned pixels; need stage -0.033 -0.182 if reset Tilting to -28 -28.00 degrees -3.8 0.6 unbinned pixels; 0.6 1.2 nm along two shift axes; -0.1 -1.4 nm on specimen axes Rate = 6.81578 A/sec Drift is low enough after shot 1 Measured defocus = -4.13 microns changed by 0.13 to target drift = 0.05 nm/sec Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.05 nm/sec Defocus = -0.610 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.29.40_batch_tomo_10_19_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 116.5 In SetExposureForMean 120, change by a factor of 1.0309 would require too small a change in exposure time Image shift -0.115 0.107 IS units -402.6 212.0 unbinned pixels; need stage -0.061 -0.169 if reset Tilting to -30 5.8 -5.8 unbinned pixels; 2.6 1.2 nm along two shift axes; 1.9 2.2 nm on specimen axes Rate = 14.4511 A/sec 6.0 2.6 unbinned pixels; 0.2 2.3 nm along two shift axes; -1.1 2.0 nm on specimen axes Rate = 11.46807 A/sec Drift is low enough after shot 2 Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.08 nm/sec Measured defocus = -3.94 microns changed by -0.06 to target drift = 0.12 nm/sec Defocus = -0.622 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.30.44_batch_tomo_10_19_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 115.1 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift -0.090 0.075 IS units -293.0 174.5 unbinned pixels; need stage -0.051 -0.126 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -0.1 3.1 unbinned pixels; 1.1 0.3 nm along two shift axes; -1.1 -0.1 nm on specimen axes Rate = 5.53569 A/sec Drift is low enough after shot 1 Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.08 nm/sec Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.03 nm/sec Defocus = -2.191 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.31.47_batch_tomo_10_19_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 125.0 In SetExposureForMean 120, exposure time changed to 1.4400 Image shift -0.155 0.222 IS units -753.9 211.7 unbinned pixels; need stage -0.050 -0.278 if reset Tilting to 12 Correcting tilt backlash 2.7 -1.9 unbinned pixels; 0.9 0.7 nm along two shift axes; 0.6 1.0 nm on specimen axes Rate = 5.753 A/sec Drift is low enough after shot 1 Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.13 nm/sec Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.05 nm/sec Defocus = -2.217 um Dewars are NOT busy filling 12 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.32.47_batch_tomo_10_19_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 114.2 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift -0.118 0.149 IS units -521.3 180.3 unbinned pixels; need stage -0.046 -0.194 if reset Tilting to -32 -32.00 degrees -1.7 -1.6 unbinned pixels; 0.3 0.8 nm along two shift axes; 0.6 -0.6 nm on specimen axes Rate = 4.1228 A/sec Drift is low enough after shot 1 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.09 nm/sec Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.03 nm/sec Defocus = -0.497 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.33.48_batch_tomo_10_19_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 123.7 In SetExposureForMean 120, change by a factor of 0.9705 would require too small a change in exposure time Image shift -0.075 -0.011 IS units -43.6 215.7 unbinned pixels; need stage -0.073 -0.026 if reset Tilting to -34 4.4 -6.6 unbinned pixels; 2.7 0.7 nm along two shift axes; 2.2 1.7 nm on specimen axes Rate = 13.93855 A/sec Drift is low enough after shot 1 Measured defocus = -4.10 microns changed by 0.10 to target drift = 0.05 nm/sec Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.08 nm/sec Defocus = -0.435 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.34.46_batch_tomo_10_19_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 121.8 In SetExposureForMean 120, change by a factor of 0.9856 would require too small a change in exposure time Image shift -0.036 -0.067 IS units 145.3 162.0 unbinned pixels; need stage -0.060 0.056 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -14.2 5.7 unbinned pixels; 3.6 4.0 nm along two shift axes; -1.6 -5.1 nm on specimen axes Rate = 26.7574 A/sec -8.8 4.6 unbinned pixels; 2.6 2.4 nm along two shift axes; -1.4 -3.2 nm on specimen axes Rate = 17.38921 A/sec -5.6 2.4 unbinned pixels; 1.5 1.6 nm along two shift axes; -0.7 -2.0 nm on specimen axes Rate = 10.71832 A/sec Drift is low enough after shot 3 Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.03 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -2.301 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.36.01_batch_tomo_10_19_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 122.1 In SetExposureForMean 120, change by a factor of 0.9830 would require too small a change in exposure time Image shift -0.161 0.213 IS units -735.9 234.1 unbinned pixels; need stage -0.058 -0.276 if reset Tilting to 16 Correcting tilt backlash 1.3 -1.9 unbinned pixels; 0.8 0.2 nm along two shift axes; 0.6 0.5 nm on specimen axes Rate = 4.04411 A/sec Drift is low enough after shot 1 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.09 nm/sec Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.08 nm/sec Defocus = -2.343 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.37.06_batch_tomo_10_19_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 120.6 In SetExposureForMean 120, change by a factor of 0.9956 would require too small a change in exposure time Image shift -0.122 0.129 IS units -469.6 209.8 unbinned pixels; need stage -0.058 -0.180 if reset Tilting to -36 -36.00 degrees 5.5 -7.3 unbinned pixels; 3.1 0.9 nm along two shift axes; 2.4 2.1 nm on specimen axes Rate = 16.00712 A/sec 0.6 1.9 unbinned pixels; 0.6 0.4 nm along two shift axes; -0.7 0.1 nm on specimen axes Rate = 3.52258 A/sec Drift is low enough after shot 2 Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.02 nm/sec Measured defocus = -3.95 microns changed by -0.05 to target drift = 0.30 nm/sec Defocus = -0.454 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.38.14_batch_tomo_10_19_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 119.9 In SetExposureForMean 120, change by a factor of 1.0016 would require too small a change in exposure time Image shift 0.002 -0.197 IS units 534.7 183.1 unbinned pixels; need stage -0.079 0.226 if reset Tilting to -38 -1.3 -0.7 unbinned pixels; 0.1 0.5 nm along two shift axes; 0.3 -0.4 nm on specimen axes Rate = 2.68276 A/sec Drift is low enough after shot 1 Measured defocus = -4.11 microns changed by 0.11 to target drift = 0.07 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.347 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.39.13_batch_tomo_10_19_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 118.2 In SetExposureForMean 120, change by a factor of 1.0162 would require too small a change in exposure time Image shift 0.017 -0.209 IS units 583.1 153.1 unbinned pixels; need stage -0.071 0.255 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -0.4 3.3 unbinned pixels; 1.2 0.3 nm along two shift axes; -1.2 -0.3 nm on specimen axes Rate = 5.92628 A/sec Drift is low enough after shot 1 Measured defocus = -3.96 microns changed by -0.04 to target drift = 0.06 nm/sec Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.09 nm/sec Defocus = -2.458 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.40.17_batch_tomo_10_19_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 118.4 In SetExposureForMean 120, change by a factor of 1.0140 would require too small a change in exposure time Image shift -0.125 0.122 IS units -455.0 224.0 unbinned pixels; need stage -0.063 -0.177 if reset Tilting to 20 Correcting tilt backlash -11.4 -4.9 unbinned pixels; 0.3 4.4 nm along two shift axes; 2.1 -3.9 nm on specimen axes Rate = 21.89082 A/sec -11.1 4.4 unbinned pixels; 2.8 3.1 nm along two shift axes; -1.2 -4.0 nm on specimen axes Rate = 20.95277 A/sec -4.2 2.2 unbinned pixels; 1.2 1.1 nm along two shift axes; -0.7 -1.5 nm on specimen axes Rate = 8.35553 A/sec Drift is low enough after shot 3 Measured defocus = -3.90 microns changed by -0.10 to target drift = 0.04 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.13 nm/sec Defocus = -2.533 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.41.27_batch_tomo_10_19_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, change by a factor of 1.0322 would require too small a change in exposure time Image shift -0.084 0.069 IS units -268.0 162.1 unbinned pixels; need stage -0.048 -0.106 if reset Tilting to -40 -40.00 degrees -0.1 -5.9 unbinned pixels; 1.9 0.7 nm along two shift axes; 2.1 0.1 nm on specimen axes Rate = 10.40145 A/sec Drift is low enough after shot 1 Measured defocus = -4.12 microns changed by 0.12 to target drift = 0.08 nm/sec Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.226 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.42.30_batch_tomo_10_19_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, change by a factor of 1.0369 would require too small a change in exposure time Image shift 0.045 -0.278 IS units 798.9 142.0 unbinned pixels; need stage -0.073 0.363 if reset Tilting to -42 4.5 -7.0 unbinned pixels; 2.8 0.7 nm along two shift axes; 2.3 1.8 nm on specimen axes Rate = 14.57334 A/sec 3.4 2.4 unbinned pixels; 0.4 1.4 nm along two shift axes; -0.9 1.1 nm on specimen axes Rate = 7.30846 A/sec Drift is low enough after shot 2 Measured defocus = -4.00 microns changed by 0.00 to target drift = 0.03 nm/sec Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.09 nm/sec Defocus = -0.251 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.43.34_batch_tomo_10_19_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 113.7 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift 0.086 -0.373 IS units 1095.9 119.7 unbinned pixels; need stage -0.075 0.516 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -17.3 1.2 unbinned pixels; 2.4 5.6 nm along two shift axes; 0.1 -6.1 nm on specimen axes Rate = 30.47471 A/sec -8.0 2.4 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.6 -2.9 nm on specimen axes Rate = 14.64826 A/sec -7.9 1.8 unbinned pixels; 1.5 2.4 nm along two shift axes; -0.4 -2.8 nm on specimen axes Rate = 14.17196 A/sec -3.9 0.8 unbinned pixels; 0.7 1.2 nm along two shift axes; -0.2 -1.4 nm on specimen axes Rate = 6.98044 A/sec Drift is low enough after shot 4 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.25 nm/sec Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.07 nm/sec Defocus = -2.496 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.44.55_batch_tomo_10_19_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 123.0 In SetExposureForMean 120, change by a factor of 0.9768 would require too small a change in exposure time Image shift -0.082 0.050 IS units -216.1 176.2 unbinned pixels; need stage -0.054 -0.089 if reset Tilting to 24 Correcting tilt backlash -8.3 -0.1 unbinned pixels; 1.0 2.8 nm along two shift axes; 0.3 -2.9 nm on specimen axes Rate = 14.63541 A/sec -4.6 6.4 unbinned pixels; 2.7 0.8 nm along two shift axes; -2.1 -1.8 nm on specimen axes Rate = 13.89406 A/sec Drift is low enough after shot 2 Measured defocus = -4.07 microns changed by 0.07 to target drift = 0.13 nm/sec Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.10 nm/sec Defocus = -2.542 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.46.00_batch_tomo_10_19_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 120.2 In SetExposureForMean 120, change by a factor of 0.9998 would require too small a change in exposure time Image shift -0.050 -0.038 IS units 53.2 170.6 unbinned pixels; need stage -0.060 0.015 if reset Tilting to -44 -44.00 degrees 2.8 -7.3 unbinned pixels; 2.8 0.1 nm along two shift axes; 2.5 1.2 nm on specimen axes Rate = 13.76359 A/sec Drift is low enough after shot 1 Measured defocus = -4.15 microns changed by 0.15 to target drift = 0.14 nm/sec Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.06 nm/sec Defocus = -0.112 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.47.04_batch_tomo_10_19_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 119.8 In SetExposureForMean 120, change by a factor of 1.0031 would require too small a change in exposure time Image shift 0.144 -0.488 IS units 1465.7 71.6 unbinned pixels; need stage -0.070 0.717 if reset Tilting to -46 3.7 -6.2 unbinned pixels; 2.5 0.5 nm along two shift axes; 2.0 1.5 nm on specimen axes Rate = 12.64356 A/sec Drift is low enough after shot 1 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.02 nm/sec Measured defocus = -3.99 microns changed by -0.01 to target drift = 0.03 nm/sec Defocus = -0.079 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.48.02_batch_tomo_10_19_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 116.8 In SetExposureForMean 120, change by a factor of 1.0288 would require too small a change in exposure time Image shift 0.178 -0.544 IS units 1649.5 32.2 unbinned pixels; need stage -0.062 0.838 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -12.1 2.1 unbinned pixels; 2.1 3.8 nm along two shift axes; -0.4 -4.3 nm on specimen axes Rate = 21.52221 A/sec -6.8 2.2 unbinned pixels; 1.5 2.0 nm along two shift axes; -0.6 -2.5 nm on specimen axes Rate = 12.57738 A/sec Drift is low enough after shot 2 Measured defocus = -3.93 microns changed by -0.07 to target drift = 0.13 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.12 nm/sec Defocus = -2.591 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.49.12_batch_tomo_10_19_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 117.7 In SetExposureForMean 120, change by a factor of 1.0206 would require too small a change in exposure time Image shift -0.044 -0.038 IS units 59.5 156.0 unbinned pixels; need stage -0.056 0.018 if reset Tilting to 28 Correcting tilt backlash -7.5 -1.4 unbinned pixels; 0.4 2.7 nm along two shift axes; 0.7 -2.6 nm on specimen axes Rate = 13.42902 A/sec Drift is low enough after shot 1 Measured defocus = -3.88 microns changed by -0.12 to target drift = 0.08 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.16 nm/sec Defocus = -2.696 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.50.17_batch_tomo_10_19_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 114.7 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift -0.024 -0.109 IS units 270.8 168.0 unbinned pixels; need stage -0.066 0.102 if reset Tilting to -48 -48.00 degrees 6.9 -6.7 unbinned pixels; 3.0 1.5 nm along two shift axes; 2.1 2.6 nm on specimen axes Rate = 16.81985 A/sec -6.2 3.4 unbinned pixels; 1.8 1.6 nm along two shift axes; -1.0 -2.3 nm on specimen axes Rate = 12.28144 A/sec Drift is low enough after shot 2 Measured defocus = -4.09 microns changed by 0.09 to target drift = 0.07 nm/sec Measured defocus = -3.83 microns changed by -0.17 to target drift = 0.32 nm/sec Defocus = -0.160 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.51.27_batch_tomo_10_19_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 122.1 In SetExposureForMean 120, change by a factor of 0.9832 would require too small a change in exposure time Image shift 0.225 -0.652 IS units 1987.1 8.2 unbinned pixels; need stage -0.064 1.050 if reset Tilting to -50 0.5 -6.2 unbinned pixels; 2.1 0.6 nm along two shift axes; 2.2 0.4 nm on specimen axes Rate = 11.00979 A/sec Drift is low enough after shot 1 Measured defocus = -4.19 microns changed by 0.19 to target drift = 0.04 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.02 nm/sec Defocus = 0.048 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.52.25_batch_tomo_10_19_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 118.5 In SetExposureForMean 120, change by a factor of 1.0133 would require too small a change in exposure time Image shift 0.261 -0.722 IS units 2213.0 -23.9 unbinned pixels; need stage -0.060 1.219 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -13.3 5.2 unbinned pixels; 3.3 3.8 nm along two shift axes; -1.4 -4.8 nm on specimen axes Rate = 24.95673 A/sec -9.7 4.3 unbinned pixels; 2.5 2.7 nm along two shift axes; -1.2 -3.5 nm on specimen axes Rate = 18.52223 A/sec -2.9 2.5 unbinned pixels; 1.2 0.7 nm along two shift axes; -0.8 -1.1 nm on specimen axes Rate = 6.68405 A/sec Drift is low enough after shot 3 Measured defocus = -3.91 microns changed by -0.09 to target drift = 0.18 nm/sec Measured defocus = -4.03 microns changed by 0.03 to target drift = 0.18 nm/sec Defocus = -2.758 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.53.42_batch_tomo_10_19_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 119.6 In SetExposureForMean 120, change by a factor of 1.0049 would require too small a change in exposure time Image shift -0.031 -0.075 IS units 171.2 156.2 unbinned pixels; need stage -0.059 0.064 if reset Tilting to 32 Correcting tilt backlash -5.5 -3.3 unbinned pixels; 0.5 2.2 nm along two shift axes; 1.3 -1.8 nm on specimen axes Rate = 11.26217 A/sec Drift is low enough after shot 1 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.75 nm/sec Measured defocus = -3.81 microns changed by -0.19 to target drift = 0.48 nm/sec Defocus = -2.902 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.54.42_batch_tomo_10_19_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 115.9 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift -0.002 -0.148 IS units 399.5 147.6 unbinned pixels; need stage -0.063 0.161 if reset Tilting to -52 -52.00 degrees 2.8 -9.0 unbinned pixels; 3.3 0.2 nm along two shift axes; 3.1 1.2 nm on specimen axes Rate = 16.50634 A/sec 4.4 2.9 unbinned pixels; 0.5 1.8 nm along two shift axes; -1.2 1.5 nm on specimen axes Rate = 9.37323 A/sec Drift is low enough after shot 2 Measured defocus = -3.97 microns changed by -0.03 to target drift = 0.08 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.13 nm/sec Defocus = 0.042 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.55.56_batch_tomo_10_19_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 122.2 In SetExposureForMean 120, change by a factor of 0.9818 would require too small a change in exposure time Image shift 0.327 -0.852 IS units 2629.4 -78.7 unbinned pixels; need stage -0.054 1.514 if reset Tilting to -54 -8.2 -5.8 unbinned pixels; 1.0 3.4 nm along two shift axes; 2.3 -2.7 nm on specimen axes Rate = 17.77504 A/sec 7.4 3.7 unbinned pixels; 0.4 2.9 nm along two shift axes; -1.5 2.5 nm on specimen axes Rate = 14.52694 A/sec 0.7 1.3 unbinned pixels; 0.4 0.4 nm along two shift axes; -0.5 0.2 nm on specimen axes Rate = 2.67824 A/sec Drift is low enough after shot 3 Measured defocus = -3.98 microns changed by -0.02 to target drift = 0.07 nm/sec Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.01 nm/sec Defocus = 0.064 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.57.05_batch_tomo_10_19_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 117.8 In SetExposureForMean 120, change by a factor of 1.0180 would require too small a change in exposure time Image shift 0.355 -0.913 IS units 2821.9 -97.9 unbinned pixels; need stage -0.053 1.703 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -12.5 -1.2 unbinned pixels; 1.1 4.3 nm along two shift axes; 0.8 -4.4 nm on specimen axes Rate = 22.11708 A/sec -26.2 8.1 unbinned pixels; 5.7 7.7 nm along two shift axes; -2.0 -9.4 nm on specimen axes Rate = 48.02197 A/sec -8.9 4.8 unbinned pixels; 2.6 2.4 nm along two shift axes; -1.4 -3.3 nm on specimen axes Rate = 17.73756 A/sec -1.3 1.5 unbinned pixels; 0.6 0.2 nm along two shift axes; -0.5 -0.5 nm on specimen axes Rate = 3.42593 A/sec Drift is low enough after shot 4 Measured defocus = -3.77 microns changed by -0.23 to target drift = 0.19 nm/sec Measured defocus = -4.48 microns changed by 0.48 to target drift = 0.22 nm/sec Defocus = -2.650 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.58.28_batch_tomo_10_19_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 119.5 In SetExposureForMean 120, change by a factor of 1.0066 would require too small a change in exposure time Image shift 0.021 -0.190 IS units 535.0 124.3 unbinned pixels; need stage -0.059 0.223 if reset Tilting to 36 Correcting tilt backlash 2.2 -5.9 unbinned pixels; 2.2 0.0 nm along two shift axes; 2.0 0.9 nm on specimen axes Rate = 11.09596 A/sec Drift is low enough after shot 1 Measured defocus = -3.87 microns changed by -0.13 to target drift = 0.31 nm/sec Measured defocus = -3.67 microns changed by -0.33 to target drift = 0.05 nm/sec Defocus = -3.115 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_03.59.28_batch_tomo_10_19_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 114.7 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.060 -0.289 IS units 842.6 112.2 unbinned pixels; need stage -0.065 0.364 if reset Tilting to -56 -56.00 degrees 6.4 -6.1 unbinned pixels; 2.8 1.4 nm along two shift axes; 1.9 2.4 nm on specimen axes Rate = 15.51259 A/sec 5.2 2.1 unbinned pixels; 0.1 2.0 nm along two shift axes; -0.9 1.8 nm on specimen axes Rate = 9.85151 A/sec Drift is low enough after shot 2 Measured defocus = -3.92 microns changed by -0.08 to target drift = 0.16 nm/sec Measured defocus = -3.83 microns changed by -0.17 to target drift = 0.20 nm/sec Defocus = -0.191 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.00.38_batch_tomo_10_19_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 120.2 In SetExposureForMean 120, change by a factor of 0.9984 would require too small a change in exposure time Image shift 0.390 -0.988 IS units 3061.2 -122.0 unbinned pixels; need stage -0.052 1.943 if reset Tilting to -58 -4.7 -4.4 unbinned pixels; 0.9 2.1 nm along two shift axes; 1.7 -1.5 nm on specimen axes Rate = 11.415 A/sec Drift is low enough after shot 1 Measured defocus = -4.24 microns changed by 0.24 to target drift = 0.12 nm/sec Measured defocus = -4.28 microns changed by 0.28 to target drift = 0.26 nm/sec Defocus = 0.334 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.01.41_batch_tomo_10_19_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 115.0 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.417 -1.042 IS units 3233.3 -144.5 unbinned pixels; need stage -0.050 2.166 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -7.5 -3.1 unbinned pixels; 0.2 2.8 nm along two shift axes; 1.3 -2.5 nm on specimen axes Rate = 14.26869 A/sec -6.3 4.7 unbinned pixels; 2.3 1.5 nm along two shift axes; -1.5 -2.4 nm on specimen axes Rate = 13.86017 A/sec Drift is low enough after shot 2 Measured defocus = -4.10 microns changed by 0.10 to target drift = 0.02 nm/sec Measured defocus = -3.71 microns changed by -0.29 to target drift = 0.40 nm/sec Defocus = -3.299 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.02.53_batch_tomo_10_19_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 123.7 In SetExposureForMean 120, change by a factor of 0.9734 would require too small a change in exposure time Image shift 0.090 -0.329 IS units 980.4 68.9 unbinned pixels; need stage -0.054 0.437 if reset Tilting to 40 Correcting tilt backlash -4.7 -5.0 unbinned pixels; 1.1 2.2 nm along two shift axes; 1.9 -1.5 nm on specimen axes Rate = 12.16361 A/sec Drift is low enough after shot 1 Measured defocus = -4.05 microns changed by 0.05 to target drift = 0.27 nm/sec Measured defocus = -4.21 microns changed by 0.21 to target drift = 0.23 nm/sec Defocus = -3.039 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.03.54_batch_tomo_10_19_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 117.9 In SetExposureForMean 120, change by a factor of 1.0218 would require too small a change in exposure time Image shift 0.138 -0.425 IS units 1286.0 29.2 unbinned pixels; need stage -0.050 0.592 if reset Tilting to -60 -60.00 degrees 4.0 -7.3 unbinned pixels; 2.9 0.4 nm along two shift axes; 2.4 1.6 nm on specimen axes Rate = 14.60308 A/sec -2.7 2.9 unbinned pixels; 1.3 0.5 nm along two shift axes; -0.9 -1.0 nm on specimen axes Rate = 6.8544 A/sec Drift is low enough after shot 2 Measured defocus = -3.83 microns changed by -0.17 to target drift = 0.08 nm/sec Measured defocus = -3.83 microns changed by -0.17 to target drift = 0.03 nm/sec Defocus = -0.004 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.05.05_batch_tomo_10_19_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 115.8 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.470 -1.143 IS units 3559.6 -191.9 unbinned pixels; need stage -0.043 2.530 if reset Tilting to -62 -6.1 -3.3 unbinned pixels; 0.4 2.4 nm along two shift axes; 1.4 -2.1 nm on specimen axes Rate = 12.31383 A/sec Drift is low enough after shot 1 Measured defocus = -4.17 microns changed by 0.17 to target drift = 0.17 nm/sec Measured defocus = -4.30 microns changed by 0.30 to target drift = 0.26 nm/sec Defocus = 0.463 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.06.04_batch_tomo_10_19_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 115.9 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.487 -1.163 IS units 3629.2 -219.3 unbinned pixels; need stage -0.036 2.749 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -8.2 0.2 unbinned pixels; 1.0 2.7 nm along two shift axes; 0.2 -2.9 nm on specimen axes Rate = 14.31672 A/sec -0.1 0.6 unbinned pixels; 0.2 0.1 nm along two shift axes; -0.2 -0.0 nm on specimen axes Rate = 1.10242 A/sec Drift is low enough after shot 2 Measured defocus = -3.52 microns changed by -0.48 to target drift = 0.02 nm/sec Measured defocus = -3.67 microns changed by -0.33 to target drift = 0.01 nm/sec Defocus = -3.844 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.07.17_batch_tomo_10_19_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 124.2 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.154 -0.444 IS units 1354.4 1.8 unbinned pixels; need stage -0.042 0.644 if reset Tilting to 44 Correcting tilt backlash -31.8 0.6 unbinned pixels; 3.9 10.4 nm along two shift axes; 0.8 -11.2 nm on specimen axes Rate = 55.78291 A/sec -4.1 5.4 unbinned pixels; 2.3 0.7 nm along two shift axes; -1.8 -1.6 nm on specimen axes Rate = 11.90284 A/sec Drift is low enough after shot 2 Measured defocus = -4.70 microns changed by 0.70 to target drift = 0.04 nm/sec Measured defocus = -4.02 microns changed by 0.02 to target drift = 0.12 nm/sec Defocus = -3.126 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.08.24_batch_tomo_10_19_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 110.7 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift 0.180 -0.506 IS units 1548.0 -9.7 unbinned pixels; need stage -0.044 0.761 if reset Tilting to -64 -64.00 degrees 3.5 -1.5 unbinned pixels; 0.9 1.0 nm along two shift axes; 0.4 1.3 nm on specimen axes Rate = 6.68372 A/sec Drift is low enough after shot 1 Measured defocus = -3.85 microns changed by -0.15 to target drift = 0.67 nm/sec Measured defocus = -3.82 microns changed by -0.18 to target drift = 0.27 nm/sec Defocus = 0.137 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.09.29_batch_tomo_10_19_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 120.1 In SetExposureForMean 120, change by a factor of 1.0029 would require too small a change in exposure time Image shift 0.490 -1.204 IS units 3745.7 -187.6 unbinned pixels; need stage -0.051 3.035 if reset Tilting to -66 -0.8 -5.1 unbinned pixels; 1.6 0.9 nm along two shift axes; 1.8 -0.1 nm on specimen axes Rate = 9.12857 A/sec Drift is low enough after shot 1 Measured defocus = -4.23 microns changed by 0.23 to target drift = 0.53 nm/sec Measured defocus = -3.76 microns changed by -0.24 to target drift = 0.13 nm/sec Defocus = 0.133 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.10.28_batch_tomo_10_19_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 112.0 In SetExposureForMean 120, exposure time changed to 2.7600 Image shift 0.521 -1.248 IS units 3895.0 -230.0 unbinned pixels; need stage -0.041 3.404 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -6.9 1.4 unbinned pixels; 1.3 2.1 nm along two shift axes; -0.3 -2.5 nm on specimen axes Rate = 12.36698 A/sec Drift is low enough after shot 1 Measured defocus = -3.15 microns changed by -0.85 to target drift = 1.18 nm/sec Measured defocus = -4.68 microns changed by 0.68 to target drift = 0.04 nm/sec Defocus = -3.289 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.11.36_batch_tomo_10_19_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 125.9 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.194 -0.514 IS units 1583.5 -39.2 unbinned pixels; need stage -0.035 0.808 if reset Tilting to 48 Correcting tilt backlash -15.1 -0.9 unbinned pixels; 1.4 5.1 nm along two shift axes; 0.8 -5.3 nm on specimen axes Rate = 26.51149 A/sec 2.5 4.9 unbinned pixels; 1.3 1.4 nm along two shift axes; -1.8 0.7 nm on specimen axes Rate = 9.70534 A/sec Drift is low enough after shot 2 Measured defocus = -4.04 microns changed by 0.04 to target drift = 0.17 nm/sec Measured defocus = -4.01 microns changed by 0.01 to target drift = 0.15 nm/sec Defocus = -3.240 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.12.42_batch_tomo_10_19_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 111.3 In SetExposureForMean 120, exposure time changed to 2.8800 Image shift 0.223 -0.589 IS units 1814.7 -48.1 unbinned pixels; need stage -0.039 0.961 if reset Tilting to -68 -68.00 degrees -5.0 -6.6 unbinned pixels; 1.6 2.4 nm along two shift axes; 2.5 -1.5 nm on specimen axes Rate = 14.65939 A/sec 3.9 3.2 unbinned pixels; 0.6 1.7 nm along two shift axes; -1.2 1.3 nm on specimen axes Rate = 8.83865 A/sec Drift is low enough after shot 2 Measured defocus = -4.00 microns changed by -0.00 to target drift = 0.40 nm/sec Measured defocus = -3.89 microns changed by -0.11 to target drift = 0.36 nm/sec Defocus = 0.018 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.13.54_batch_tomo_10_19_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 117.9 In SetExposureForMean 120, exposure time changed to 3.0000 Image shift 0.571 -1.323 IS units 4146.8 -294.4 unbinned pixels; need stage -0.026 3.940 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 0.55 microns changed by -4.55 to target drift = 0.41 nm/sec Focus area changed from stored state, axis position -2.70 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_11.st Running script 1 at item 21 (-479.13, -188.09) 04:14:56 Refine ZLP: additional offset = -0.3 eV; total offset = -14.7 eV Item 21: Stage: -479.13 -188.09 -223 Label: 21 Note: Sec 20 - anchor_maps.map Target defocus is: -4.00 um Target defocus is: -4.25 um Target defocus = -4.25 Trimming A by 25 75, reference by 21 80 Angle = -4.40, increment = 0.60, shift = -24.7 -8.5 pixels, -0.058 um Y Trimming A by 21 80, reference by 21 80 Angle = -3.20, increment = 1.80, shift = -96.7 -22.7 pixels, -0.225 um Y Trimming A by 20 75, reference by 21 80 Angle = 0.41, increment = 5.41, shift = -337.8 -63.0 pixels, -0.782 um Y Trimming A by 25 75, reference by 21 80 Angle = 3.00, increment = 8.00, shift = -488.0 -89.2 pixels, -1.130 um Y Rough eucentricity: changing Z by 8.10 to -215.30, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 25 80, reference by 25 80 Angle = 18.00, shift = -57.2, -2.2 pixels, 0.010, -0.130 um, IS = -0.010, 0.130 Trimming A by 20 80, reference by 25 80 Angle = 11.00, shift = -139.4, -18.2 pixels, -0.006, -0.321 um, IS = -0.004, 0.451 Trimming A by 21 80, reference by 21 80 Angle = 4.00, shift = -102.8, -12.2 pixels, -0.001, -0.236 um, IS = -0.003, 0.687 Trimming A by 36 20 20 75, reference by 50 20 20 75 Angle = -4.00, shift = -34.5, -21.1 pixels, -0.039, -0.084 um, IS = 0.036, 0.771 Trimming A by 21 80, reference by 21 80 Angle = -11.00, shift = -55.9, -22.2 pixels, -0.036, -0.132 um, IS = 0.073, 0.904 Trimming A by 21 80, reference by 21 80 Angle = -18.00, shift = -28.2, -0.4 pixels, 0.006, -0.064 um, IS = 0.066, 0.968 Trimming A by 21 80, reference by 21 80 Angle = -24.00, shift = -17.7, 0.5 pixels, 0.006, -0.040 um, IS = 0.061, 1.008 General solution delZ 1.26, delY 2.05, yZero 0.72 solution with assumed YZero 0.73: delZ 1.26, delY 2.14 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 1.26 microns and lateral displacement of -2.05 microns Trimming A by 37 20 20 80, reference by 53 20 20 65 Disparity in stage position after aligning to center of map frame: 0.14 -1.47 Trimming A by 67 20 20 65, reference by 53 20 20 65 Disparity in stage position after aligning to target position: 0.55 -1.84 (2nd move error 0.04 0.15) Disparity in stage position after aligning to map item itself: 0.15 -1.31 (align shift -0.03 -0.03) Finished aligning to item in 3 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -478.98 -189.52 -216.55 1.0 4.0 unbinned pixels; 1.2 0.8 nm along two shift axes; -1.4 0.2 nm on specimen axes Rate = 7.23498 A/sec Drift is low enough after shot 1 Measured defocus = 1.20 microns changed by -5.45 to target drift = 0.11 nm/sec Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.24 nm/sec Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.12 nm/sec Defocus = -1.559 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.20.16_batch_tomo_11_21_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 90.4 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift 0.055 -0.108 IS units 346.3 -47.8 unbinned pixels; need stage 0.006 0.126 if reset Tilting to -8 Correcting tilt backlash 10.6 1.1 unbinned pixels; 0.9 3.6 nm along two shift axes; -0.7 3.7 nm on specimen axes Rate = 18.69544 A/sec 6.3 4.9 unbinned pixels; 0.9 2.7 nm along two shift axes; -1.9 2.1 nm on specimen axes Rate = 14.07195 A/sec 4.5 1.7 unbinned pixels; 0.1 1.7 nm along two shift axes; -0.7 1.5 nm on specimen axes Rate = 8.41421 A/sec Drift is low enough after shot 3 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -1.638 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.21.26_batch_tomo_11_21_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 117.7 In SetExposureForMean 120, change by a factor of 1.0197 would require too small a change in exposure time Image shift -0.011 -0.083 IS units 213.1 110.0 unbinned pixels; need stage -0.044 0.073 if reset Tilting to -12 -12.00 degrees -6.6 6.1 unbinned pixels; 2.8 1.5 nm along two shift axes; -2.0 -2.5 nm on specimen axes Rate = 15.84956 A/sec -1.0 3.7 unbinned pixels; 1.3 0.1 nm along two shift axes; -1.3 -0.4 nm on specimen axes Rate = 6.69367 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.08 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -1.485 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.22.31_batch_tomo_11_21_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 117.2 In SetExposureForMean 120, change by a factor of 1.0241 would require too small a change in exposure time Image shift -0.046 -0.057 IS units 109.7 178.9 unbinned pixels; need stage -0.065 0.034 if reset Tilting to -14 3.0 1.7 unbinned pixels; 0.2 1.2 nm along two shift axes; -0.7 1.0 nm on specimen axes Rate = 6.12896 A/sec Drift is low enough after shot 1 Measured defocus = -4.41 microns changed by 0.16 to target drift = 0.13 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -1.324 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.23.28_batch_tomo_11_21_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 117.1 In SetExposureForMean 120, change by a factor of 1.0254 would require too small a change in exposure time Image shift -0.097 0.027 IS units -167.5 238.0 unbinned pixels; need stage -0.077 -0.069 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 7.9 -0.6 unbinned pixels; 1.1 2.5 nm along two shift axes; -0.0 2.8 nm on specimen axes Rate = 13.83056 A/sec Drift is low enough after shot 1 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.14 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.09 nm/sec Defocus = -1.694 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.24.35_batch_tomo_11_21_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 117.6 In SetExposureForMean 120, change by a factor of 1.0208 would require too small a change in exposure time Image shift -0.017 -0.175 IS units 457.5 213.9 unbinned pixels; need stage -0.088 0.156 if reset Tilting to -4 Correcting tilt backlash -4.3 1.7 unbinned pixels; 1.1 1.2 nm along two shift axes; -0.5 -1.6 nm on specimen axes Rate = 8.14963 A/sec Drift is low enough after shot 1 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -1.767 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.25.34_batch_tomo_11_21_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 117.1 In SetExposureForMean 120, change by a factor of 1.0246 would require too small a change in exposure time Image shift -0.018 -0.186 IS units 484.8 226.2 unbinned pixels; need stage -0.093 0.164 if reset Tilting to -16 -16.00 degrees -6.6 1.1 unbinned pixels; 1.1 2.1 nm along two shift axes; -0.2 -2.3 nm on specimen axes Rate = 11.71234 A/sec Drift is low enough after shot 1 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.07 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -1.241 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.26.34_batch_tomo_11_21_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 116.4 In SetExposureForMean 120, change by a factor of 1.0316 would require too small a change in exposure time Image shift -0.119 -0.042 IS units -2.8 365.7 unbinned pixels; need stage -0.126 -0.013 if reset Tilting to -18 8.1 -0.4 unbinned pixels; 1.1 2.6 nm along two shift axes; -0.1 2.9 nm on specimen axes Rate = 14.23999 A/sec 5.8 0.5 unbinned pixels; 0.5 2.0 nm along two shift axes; -0.4 2.0 nm on specimen axes Rate = 10.28287 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.17 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -1.174 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.27.37_batch_tomo_11_21_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 115.7 In SetExposureForMean 120, change by a factor of 1.0379 would require too small a change in exposure time Image shift -0.106 -0.075 IS units 98.3 359.5 unbinned pixels; need stage -0.127 0.024 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 13.1 0.0 unbinned pixels; 1.5 4.3 nm along two shift axes; -0.4 4.6 nm on specimen axes Rate = 23.01042 A/sec 6.7 4.6 unbinned pixels; 0.7 2.8 nm along two shift axes; -1.8 2.2 nm on specimen axes Rate = 14.32157 A/sec 5.4 0.9 unbinned pixels; 0.3 1.9 nm along two shift axes; -0.5 1.9 nm on specimen axes Rate = 9.65501 A/sec Drift is low enough after shot 3 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -1.800 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.28.50_batch_tomo_11_21_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 116.8 In SetExposureForMean 120, change by a factor of 1.0270 would require too small a change in exposure time Image shift -0.084 -0.144 IS units 306.4 365.5 unbinned pixels; need stage -0.135 0.097 if reset Tilting to 0 Correcting tilt backlash -3.1 1.2 unbinned pixels; 0.8 0.9 nm along two shift axes; -0.3 -1.1 nm on specimen axes Rate = 5.85917 A/sec Drift is low enough after shot 1 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.12 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.09 nm/sec Defocus = -1.864 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.29.51_batch_tomo_11_21_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 116.5 In SetExposureForMean 120, change by a factor of 1.0297 would require too small a change in exposure time Image shift -0.088 -0.121 IS units 242.4 355.5 unbinned pixels; need stage -0.130 0.074 if reset Tilting to -20 -20.00 degrees -4.4 -0.2 unbinned pixels; 0.5 1.5 nm along two shift axes; 0.2 -1.5 nm on specimen axes Rate = 7.73501 A/sec Drift is low enough after shot 1 Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.04 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.138 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.30.51_batch_tomo_11_21_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 114.9 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift -0.107 -0.151 IS units 302.8 434.1 unbinned pixels; need stage -0.159 0.099 if reset Tilting to -22 5.6 -1.8 unbinned pixels; 1.3 1.6 nm along two shift axes; 0.5 2.0 nm on specimen axes Rate = 10.31359 A/sec Drift is low enough after shot 1 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.14 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.10 nm/sec Defocus = -1.099 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.31.48_batch_tomo_11_21_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 122.9 In SetExposureForMean 120, change by a factor of 0.9772 would require too small a change in exposure time Image shift -0.087 -0.187 IS units 419.9 414.0 unbinned pixels; need stage -0.155 0.146 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 10.6 1.5 unbinned pixels; 0.7 3.7 nm along two shift axes; -0.8 3.7 nm on specimen axes Rate = 18.73998 A/sec 7.5 2.5 unbinned pixels; 0.1 2.8 nm along two shift axes; -1.1 2.5 nm on specimen axes Rate = 13.8543 A/sec Drift is low enough after shot 2 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -1.944 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.32.56_batch_tomo_11_21_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 125.1 In SetExposureForMean 120, exposure time changed to 1.5600 Image shift -0.141 -0.084 IS units 88.1 462.7 unbinned pixels; need stage -0.162 0.016 if reset Tilting to 4 Correcting tilt backlash -10.5 1.3 unbinned pixels; 1.7 3.3 nm along two shift axes; -0.1 -3.7 nm on specimen axes Rate = 18.597 A/sec -9.8 2.8 unbinned pixels; 2.1 2.9 nm along two shift axes; -0.7 -3.5 nm on specimen axes Rate = 17.76099 A/sec -8.1 3.0 unbinned pixels; 1.9 2.3 nm along two shift axes; -0.8 -2.9 nm on specimen axes Rate = 15.1604 A/sec -7.1 2.4 unbinned pixels; 1.6 2.1 nm along two shift axes; -0.6 -2.6 nm on specimen axes Rate = 13.10976 A/sec Drift is low enough after shot 4 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.12 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.12 nm/sec Defocus = -2.019 um Dewars are NOT busy filling 13 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.34.13_batch_tomo_11_21_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 115.4 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift -0.154 -0.056 IS units -0.3 471.6 unbinned pixels; need stage -0.162 -0.015 if reset Tilting to -24 -24.00 degrees -0.3 -0.0 unbinned pixels; 0.0 0.1 nm along two shift axes; 0.0 -0.1 nm on specimen axes Rate = 0.5254 A/sec Drift is low enough after shot 1 Measured defocus = -4.36 microns changed by 0.11 to target drift = 0.04 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.976 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.35.13_batch_tomo_11_21_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 122.0 In SetExposureForMean 120, change by a factor of 0.9848 would require too small a change in exposure time Image shift -0.079 -0.273 IS units 661.7 473.8 unbinned pixels; need stage -0.183 0.239 if reset Tilting to -26 6.9 -0.8 unbinned pixels; 1.1 2.2 nm along two shift axes; 0.1 2.5 nm on specimen axes Rate = 12.22288 A/sec Drift is low enough after shot 1 Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.12 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -0.971 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.36.11_batch_tomo_11_21_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 120.7 In SetExposureForMean 120, change by a factor of 0.9952 would require too small a change in exposure time Image shift -0.067 -0.292 IS units 725.2 461.4 unbinned pixels; need stage -0.181 0.269 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees 10.0 2.3 unbinned pixels; 0.4 3.6 nm along two shift axes; -1.1 3.4 nm on specimen axes Rate = 18.05532 A/sec 7.0 1.0 unbinned pixels; 0.5 2.4 nm along two shift axes; -0.6 2.4 nm on specimen axes Rate = 12.44774 A/sec Drift is low enough after shot 2 Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.13 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.09 nm/sec Defocus = -2.093 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.37.19_batch_tomo_11_21_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 123.4 In SetExposureForMean 120, change by a factor of 0.9723 would require too small a change in exposure time Image shift -0.174 -0.079 IS units 43.8 548.5 unbinned pixels; need stage -0.190 -0.002 if reset Tilting to 8 Correcting tilt backlash -26.1 8.0 unbinned pixels; 5.7 7.7 nm along two shift axes; -2.0 -9.4 nm on specimen axes Rate = 47.86696 A/sec -20.5 6.3 unbinned pixels; 4.5 6.0 nm along two shift axes; -1.6 -7.4 nm on specimen axes Rate = 37.52444 A/sec -9.7 3.7 unbinned pixels; 2.4 2.8 nm along two shift axes; -1.0 -3.5 nm on specimen axes Rate = 18.20997 A/sec -9.6 3.6 unbinned pixels; 2.3 2.7 nm along two shift axes; -1.0 -3.5 nm on specimen axes Rate = 17.9309 A/sec -7.8 2.7 unbinned pixels; 1.8 2.3 nm along two shift axes; -0.7 -2.8 nm on specimen axes Rate = 14.37952 A/sec -6.4 2.6 unbinned pixels; 1.6 1.8 nm along two shift axes; -0.7 -2.3 nm on specimen axes Rate = 12.05844 A/sec Drift is low enough after shot 6 Measured defocus = -4.17 microns changed by -0.08 to target drift = 0.15 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.13 nm/sec Defocus = -2.165 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.38.48_batch_tomo_11_21_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 122.3 In SetExposureForMean 120, change by a factor of 0.9814 would require too small a change in exposure time Image shift -0.200 -0.035 IS units -103.5 577.4 unbinned pixels; need stage -0.196 -0.056 if reset Tilting to -28 -28.00 degrees 0.0 -1.5 unbinned pixels; 0.5 0.2 nm along two shift axes; 0.5 0.1 nm on specimen axes Rate = 2.66831 A/sec Drift is low enough after shot 1 Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.05 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -0.936 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.39.50_batch_tomo_11_21_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 119.1 In SetExposureForMean 120, change by a factor of 1.0085 would require too small a change in exposure time Image shift -0.054 -0.373 IS units 957.8 503.7 unbinned pixels; need stage -0.203 0.365 if reset Tilting to -30 0.7 1.0 unbinned pixels; 0.2 0.3 nm along two shift axes; -0.4 0.2 nm on specimen axes Rate = 2.06768 A/sec Drift is low enough after shot 1 Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.07 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.914 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.40.47_batch_tomo_11_21_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 117.7 In SetExposureForMean 120, change by a factor of 1.0204 would require too small a change in exposure time Image shift -0.068 -0.346 IS units 871.2 515.9 unbinned pixels; need stage -0.204 0.336 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees 13.5 -0.1 unbinned pixels; 1.6 4.5 nm along two shift axes; -0.4 4.7 nm on specimen axes Rate = 23.73319 A/sec 12.9 1.8 unbinned pixels; 0.9 4.5 nm along two shift axes; -1.0 4.5 nm on specimen axes Rate = 22.86966 A/sec 7.8 2.2 unbinned pixels; 0.2 2.8 nm along two shift axes; -1.0 2.7 nm on specimen axes Rate = 14.2141 A/sec 7.5 3.2 unbinned pixels; 0.2 2.9 nm along two shift axes; -1.3 2.5 nm on specimen axes Rate = 14.37663 A/sec 2.3 1.2 unbinned pixels; 0.1 0.9 nm along two shift axes; -0.5 0.8 nm on specimen axes Rate = 4.55292 A/sec Drift is low enough after shot 5 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.05 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -2.245 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.42.19_batch_tomo_11_21_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 121.0 In SetExposureForMean 120, change by a factor of 0.9920 would require too small a change in exposure time Image shift -0.203 -0.092 IS units 48.6 638.9 unbinned pixels; need stage -0.221 -0.004 if reset Tilting to 12 Correcting tilt backlash -17.7 -1.2 unbinned pixels; 1.7 6.0 nm along two shift axes; 1.0 -6.2 nm on specimen axes Rate = 31.09767 A/sec -9.3 6.9 unbinned pixels; 3.4 2.3 nm along two shift axes; -2.1 -3.5 nm on specimen axes Rate = 20.36516 A/sec -6.3 3.3 unbinned pixels; 1.8 1.7 nm along two shift axes; -1.0 -2.3 nm on specimen axes Rate = 12.43246 A/sec Drift is low enough after shot 3 Measured defocus = -4.20 microns changed by -0.05 to target drift = 0.15 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.12 nm/sec Defocus = -2.288 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.43.30_batch_tomo_11_21_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 119.6 In SetExposureForMean 120, change by a factor of 1.0034 would require too small a change in exposure time Image shift -0.171 -0.141 IS units 213.6 598.5 unbinned pixels; need stage -0.213 0.057 if reset Tilting to -32 -32.00 degrees 0.3 0.2 unbinned pixels; 0.0 0.1 nm along two shift axes; -0.1 0.1 nm on specimen axes Rate = 0.61378 A/sec Drift is low enough after shot 1 Measured defocus = -4.33 microns changed by 0.08 to target drift = 0.06 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.836 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.44.33_batch_tomo_11_21_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, change by a factor of 1.0348 would require too small a change in exposure time Image shift -0.068 -0.398 IS units 1010.7 565.0 unbinned pixels; need stage -0.226 0.400 if reset Tilting to -34 3.5 -2.0 unbinned pixels; 1.1 0.9 nm along two shift axes; 0.6 1.3 nm on specimen axes Rate = 7.1317 A/sec Drift is low enough after shot 1 Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.10 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -0.826 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.45.30_batch_tomo_11_21_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 113.9 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift -0.051 -0.431 IS units 1116.3 549.8 unbinned pixels; need stage -0.224 0.455 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -4.8 3.8 unbinned pixels; 1.8 1.1 nm along two shift axes; -1.2 -1.8 nm on specimen axes Rate = 10.72278 A/sec Drift is low enough after shot 1 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.08 nm/sec Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.05 nm/sec Defocus = -2.348 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.46.34_batch_tomo_11_21_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 126.5 In SetExposureForMean 120, exposure time changed to 1.6800 Image shift -0.181 -0.160 IS units 255.5 643.7 unbinned pixels; need stage -0.229 0.072 if reset Tilting to 16 Correcting tilt backlash 2.4 -5.4 unbinned pixels; 2.1 0.2 nm along two shift axes; 1.8 1.0 nm on specimen axes Rate = 10.41346 A/sec Drift is low enough after shot 1 Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.07 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -2.367 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.47.34_batch_tomo_11_21_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 116.4 In SetExposureForMean 120, change by a factor of 1.0315 would require too small a change in exposure time Image shift -0.125 -0.241 IS units 528.6 570.8 unbinned pixels; need stage -0.213 0.175 if reset Tilting to -36 -36.00 degrees 4.5 -5.7 unbinned pixels; 2.4 0.8 nm along two shift axes; 1.9 1.8 nm on specimen axes Rate = 12.78765 A/sec Drift is low enough after shot 1 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -0.750 um Dewars are NOT busy filling 14 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.48.36_batch_tomo_11_21_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 111.9 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift -0.007 -0.535 IS units 1442.7 528.8 unbinned pixels; need stage -0.226 0.609 if reset Tilting to -38 6.0 -0.7 unbinned pixels; 0.9 1.9 nm along two shift axes; 0.1 2.1 nm on specimen axes Rate = 10.52585 A/sec Drift is low enough after shot 1 Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.09 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -0.746 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.49.34_batch_tomo_11_21_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0229 would require too small a change in exposure time Image shift 0.001 -0.548 IS units 1485.2 519.4 unbinned pixels; need stage -0.224 0.645 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -12.3 2.3 unbinned pixels; 2.2 3.8 nm along two shift axes; -0.4 -4.4 nm on specimen axes Rate = 21.93035 A/sec -4.6 3.2 unbinned pixels; 1.6 1.1 nm along two shift axes; -1.0 -1.7 nm on specimen axes Rate = 9.80613 A/sec Drift is low enough after shot 2 Measured defocus = -4.11 microns changed by -0.14 to target drift = 0.12 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.08 nm/sec Defocus = -2.513 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.50.44_batch_tomo_11_21_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 122.7 In SetExposureForMean 120, change by a factor of 0.9791 would require too small a change in exposure time Image shift -0.177 -0.179 IS units 310.1 651.8 unbinned pixels; need stage -0.234 0.093 if reset Tilting to 20 Correcting tilt backlash 6.1 -0.7 unbinned pixels; 0.9 2.0 nm along two shift axes; 0.1 2.2 nm on specimen axes Rate = 10.83254 A/sec Drift is low enough after shot 1 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.12 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -2.525 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.51.44_batch_tomo_11_21_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 120.5 In SetExposureForMean 120, change by a factor of 0.9965 would require too small a change in exposure time Image shift -0.136 -0.290 IS units 652.7 645.3 unbinned pixels; need stage -0.242 0.223 if reset Tilting to -40 -40.00 degrees 4.3 -4.9 unbinned pixels; 2.1 0.8 nm along two shift axes; 1.6 1.7 nm on specimen axes Rate = 11.45871 A/sec Drift is low enough after shot 1 Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.06 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.700 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.52.46_batch_tomo_11_21_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 114.7 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift 0.035 -0.642 IS units 1772.8 515.9 unbinned pixels; need stage -0.232 0.796 if reset Tilting to -42 4.3 -5.7 unbinned pixels; 2.4 0.7 nm along two shift axes; 1.9 1.7 nm on specimen axes Rate = 12.61208 A/sec Drift is low enough after shot 1 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -0.719 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.53.44_batch_tomo_11_21_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0079 would require too small a change in exposure time Image shift 0.062 -0.694 IS units 1940.7 491.5 unbinned pixels; need stage -0.229 0.902 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -5.9 2.1 unbinned pixels; 1.4 1.7 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 11.00832 A/sec Drift is low enough after shot 1 Measured defocus = -4.12 microns changed by -0.13 to target drift = 0.19 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.14 nm/sec Defocus = -2.653 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.54.49_batch_tomo_11_21_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 125.9 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift -0.184 -0.173 IS units 288.1 665.7 unbinned pixels; need stage -0.238 0.087 if reset Tilting to 24 Correcting tilt backlash -5.4 -0.9 unbinned pixels; 0.3 1.9 nm along two shift axes; 0.5 -1.9 nm on specimen axes Rate = 9.63063 A/sec Drift is low enough after shot 1 Measured defocus = -4.20 microns changed by -0.05 to target drift = 0.11 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.700 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.55.49_batch_tomo_11_21_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 115.5 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift -0.146 -0.285 IS units 627.2 669.8 unbinned pixels; need stage -0.250 0.219 if reset Tilting to -44 -44.00 degrees 6.1 -5.4 unbinned pixels; 2.5 1.4 nm along two shift axes; 1.7 2.3 nm on specimen axes Rate = 14.30965 A/sec 3.2 2.9 unbinned pixels; 0.6 1.4 nm along two shift axes; -1.1 1.0 nm on specimen axes Rate = 7.65686 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.06 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -0.651 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.56.58_batch_tomo_11_21_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 115.9 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.106 -0.790 IS units 2244.1 461.9 unbinned pixels; need stage -0.228 1.082 if reset Tilting to -46 1.8 -1.3 unbinned pixels; 0.6 0.5 nm along two shift axes; 0.4 0.7 nm on specimen axes Rate = 3.88881 A/sec Drift is low enough after shot 1 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.11 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -0.677 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.57.56_batch_tomo_11_21_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 119.6 In SetExposureForMean 120, change by a factor of 1.0048 would require too small a change in exposure time Image shift 0.127 -0.830 IS units 2373.2 443.5 unbinned pixels; need stage -0.226 1.187 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -7.1 2.0 unbinned pixels; 1.5 2.1 nm along two shift axes; -0.5 -2.5 nm on specimen axes Rate = 12.91143 A/sec Drift is low enough after shot 1 Measured defocus = -4.09 microns changed by -0.16 to target drift = 0.20 nm/sec Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.13 nm/sec Defocus = -2.846 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_04.59.01_batch_tomo_11_21_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 127.9 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift -0.181 -0.197 IS units 354.5 680.4 unbinned pixels; need stage -0.245 0.115 if reset Tilting to 28 Correcting tilt backlash -6.7 -1.8 unbinned pixels; 0.2 2.4 nm along two shift axes; 0.8 -2.3 nm on specimen axes Rate = 12.12674 A/sec Drift is low enough after shot 1 Measured defocus = -4.21 microns changed by -0.04 to target drift = 0.11 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -2.880 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.00.02_batch_tomo_11_21_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 117.2 In SetExposureForMean 120, change by a factor of 1.0258 would require too small a change in exposure time Image shift -0.141 -0.310 IS units 699.5 679.8 unbinned pixels; need stage -0.255 0.255 if reset Tilting to -48 -48.00 degrees 3.5 -7.2 unbinned pixels; 2.8 0.3 nm along two shift axes; 2.4 1.5 nm on specimen axes Rate = 14.02078 A/sec 6.0 3.2 unbinned pixels; 0.4 2.4 nm along two shift axes; -1.3 2.0 nm on specimen axes Rate = 11.99598 A/sec Drift is low enough after shot 2 Measured defocus = -4.30 microns changed by 0.05 to target drift = 0.08 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -0.630 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.01.11_batch_tomo_11_21_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 108.8 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.172 -0.923 IS units 2668.4 410.8 unbinned pixels; need stage -0.223 1.390 if reset Tilting to -50 0.5 -6.1 unbinned pixels; 2.1 0.5 nm along two shift axes; 2.1 0.4 nm on specimen axes Rate = 10.71697 A/sec Drift is low enough after shot 1 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.11 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -0.656 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.02.09_batch_tomo_11_21_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 117.9 In SetExposureForMean 120, change by a factor of 1.0201 would require too small a change in exposure time Image shift 0.213 -1.008 IS units 2941.5 380.2 unbinned pixels; need stage -0.221 1.599 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -11.8 4.7 unbinned pixels; 3.0 3.3 nm along two shift axes; -1.3 -4.3 nm on specimen axes Rate = 22.29818 A/sec -7.9 2.3 unbinned pixels; 1.7 2.3 nm along two shift axes; -0.6 -2.8 nm on specimen axes Rate = 14.36585 A/sec -8.2 2.1 unbinned pixels; 1.7 2.5 nm along two shift axes; -0.5 -2.9 nm on specimen axes Rate = 14.87019 A/sec -3.9 2.5 unbinned pixels; 1.3 1.0 nm along two shift axes; -0.8 -1.5 nm on specimen axes Rate = 8.17459 A/sec Drift is low enough after shot 4 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.14 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.10 nm/sec Defocus = -3.000 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.03.32_batch_tomo_11_21_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 127.9 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift -0.156 -0.277 IS units 596.2 687.2 unbinned pixels; need stage -0.255 0.218 if reset Tilting to 32 Correcting tilt backlash -8.3 -5.7 unbinned pixels; 0.9 3.4 nm along two shift axes; 2.3 -2.7 nm on specimen axes Rate = 17.74946 A/sec -3.2 3.6 unbinned pixels; 1.6 0.6 nm along two shift axes; -1.2 -1.2 nm on specimen axes Rate = 8.40181 A/sec Drift is low enough after shot 2 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.08 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -3.042 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.04.38_batch_tomo_11_21_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 117.2 In SetExposureForMean 120, change by a factor of 1.0266 would require too small a change in exposure time Image shift -0.098 -0.407 IS units 1005.0 655.0 unbinned pixels; need stage -0.256 0.394 if reset Tilting to -52 -52.00 degrees 6.6 -6.4 unbinned pixels; 2.9 1.4 nm along two shift axes; 2.0 2.5 nm on specimen axes Rate = 16.08255 A/sec 4.6 2.2 unbinned pixels; 0.2 1.8 nm along two shift axes; -0.9 1.5 nm on specimen axes Rate = 8.93633 A/sec Drift is low enough after shot 2 Measured defocus = -4.31 microns changed by 0.06 to target drift = 0.08 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -0.595 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.05.48_batch_tomo_11_21_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 106.9 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.258 -1.098 IS units 3230.1 342.3 unbinned pixels; need stage -0.217 1.837 if reset Tilting to -54 2.2 -4.2 unbinned pixels; 1.7 0.2 nm along two shift axes; 1.4 0.9 nm on specimen axes Rate = 8.38128 A/sec Drift is low enough after shot 1 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.11 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -0.626 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.06.51_batch_tomo_11_21_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 114.0 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.288 -1.175 IS units 3466.1 334.2 unbinned pixels; need stage -0.222 2.067 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -19.2 4.3 unbinned pixels; 3.7 5.8 nm along two shift axes; -0.9 -6.9 nm on specimen axes Rate = 34.41318 A/sec -8.2 3.6 unbinned pixels; 2.2 2.3 nm along two shift axes; -1.0 -3.0 nm on specimen axes Rate = 15.71247 A/sec -7.9 2.3 unbinned pixels; 1.7 2.3 nm along two shift axes; -0.6 -2.8 nm on specimen axes Rate = 14.35273 A/sec -5.8 1.9 unbinned pixels; 1.3 1.7 nm along two shift axes; -0.5 -2.1 nm on specimen axes Rate = 10.64991 A/sec Drift is low enough after shot 4 Measured defocus = -4.13 microns changed by -0.12 to target drift = 0.16 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.12 nm/sec Defocus = -3.160 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.08.15_batch_tomo_11_21_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 132.8 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift -0.117 -0.359 IS units 857.9 659.4 unbinned pixels; need stage -0.253 0.340 if reset Tilting to 36 Correcting tilt backlash -8.6 -6.5 unbinned pixels; 1.2 3.6 nm along two shift axes; 2.5 -2.8 nm on specimen axes Rate = 18.97684 A/sec -3.5 2.6 unbinned pixels; 1.3 0.8 nm along two shift axes; -0.8 -1.3 nm on specimen axes Rate = 7.62356 A/sec Drift is low enough after shot 2 Measured defocus = -4.22 microns changed by -0.03 to target drift = 0.08 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -3.195 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.09.21_batch_tomo_11_21_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 114.9 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift -0.068 -0.473 IS units 1215.0 634.6 unbinned pixels; need stage -0.256 0.506 if reset Tilting to -56 -56.00 degrees 6.0 -6.6 unbinned pixels; 2.9 1.2 nm along two shift axes; 2.1 2.3 nm on specimen axes Rate = 15.64367 A/sec 5.8 2.2 unbinned pixels; 0.1 2.2 nm along two shift axes; -1.0 2.0 nm on specimen axes Rate = 10.98241 A/sec Drift is low enough after shot 2 Measured defocus = -4.34 microns changed by 0.09 to target drift = 0.10 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -0.543 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.10.31_batch_tomo_11_21_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 108.4 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift 0.324 -1.239 IS units 3675.3 296.2 unbinned pixels; need stage -0.215 2.307 if reset Tilting to -58 0.5 -4.7 unbinned pixels; 1.6 0.4 nm along two shift axes; 1.6 0.3 nm on specimen axes Rate = 8.32718 A/sec Drift is low enough after shot 1 Measured defocus = -4.23 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -0.570 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.11.30_batch_tomo_11_21_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 113.0 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.353 -1.298 IS units 3864.9 273.5 unbinned pixels; need stage -0.213 2.562 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -17.9 1.3 unbinned pixels; 2.5 5.8 nm along two shift axes; 0.1 -6.3 nm on specimen axes Rate = 31.45472 A/sec -8.7 2.9 unbinned pixels; 2.0 2.5 nm along two shift axes; -0.8 -3.1 nm on specimen axes Rate = 15.99298 A/sec -7.6 1.8 unbinned pixels; 1.5 2.3 nm along two shift axes; -0.4 -2.7 nm on specimen axes Rate = 13.74762 A/sec Drift is low enough after shot 3 Measured defocus = -4.12 microns changed by -0.13 to target drift = 0.17 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.11 nm/sec Defocus = -3.332 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.12.48_batch_tomo_11_21_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 134.4 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift -0.076 -0.426 IS units 1079.3 613.8 unbinned pixels; need stage -0.244 0.459 if reset Tilting to 40 Correcting tilt backlash -8.1 -8.3 unbinned pixels; 1.8 3.7 nm along two shift axes; 3.1 -2.6 nm on specimen axes Rate = 20.48284 A/sec -2.6 1.6 unbinned pixels; 0.8 0.7 nm along two shift axes; -0.5 -1.0 nm on specimen axes Rate = 5.37727 A/sec Drift is low enough after shot 2 Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.09 nm/sec Measured defocus = -4.24 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -3.343 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.13.55_batch_tomo_11_21_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 116.5 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift -0.026 -0.543 IS units 1446.0 588.7 unbinned pixels; need stage -0.247 0.643 if reset Tilting to -60 -60.00 degrees 4.1 -6.7 unbinned pixels; 2.7 0.6 nm along two shift axes; 2.2 1.7 nm on specimen axes Rate = 13.89635 A/sec Drift is low enough after shot 1 Measured defocus = -4.36 microns changed by 0.11 to target drift = 0.07 nm/sec Measured defocus = -4.25 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -0.464 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.15.00_batch_tomo_11_21_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 105.9 In SetExposureForMean 120, exposure time changed to 2.8800 Image shift 0.397 -1.382 IS units 4134.9 233.8 unbinned pixels; need stage -0.208 2.909 if reset Tilting to -62 -2.5 -4.7 unbinned pixels; 1.3 1.4 nm along two shift axes; 1.7 -0.7 nm on specimen axes Rate = 9.52265 A/sec Drift is low enough after shot 1 Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.06 nm/sec Measured defocus = -4.28 microns changed by 0.03 to target drift = 0.05 nm/sec Defocus = -0.502 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.16.04_batch_tomo_11_21_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 96.1 In SetExposureForMean 120, exposure time changed to 3.2400 Trimming A by 95 115, reference by 20 20 Image shift 0.437 -1.465 IS units 4398.5 203.7 unbinned pixels; need stage -0.205 3.299 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -16.1 3.0 unbinned pixels; 2.9 5.0 nm along two shift axes; -0.5 -5.7 nm on specimen axes Rate = 28.75583 A/sec -10.7 3.1 unbinned pixels; 2.3 3.2 nm along two shift axes; -0.8 -3.9 nm on specimen axes Rate = 19.58833 A/sec -7.8 2.5 unbinned pixels; 1.8 2.3 nm along two shift axes; -0.6 -2.8 nm on specimen axes Rate = 14.41757 A/sec -6.9 2.1 unbinned pixels; 1.5 2.0 nm along two shift axes; -0.5 -2.5 nm on specimen axes Rate = 12.58378 A/sec Drift is low enough after shot 4 Measured defocus = -4.19 microns changed by -0.06 to target drift = 0.14 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.13 nm/sec Defocus = -3.401 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.17.29_batch_tomo_11_21_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 148.6 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift -0.024 -0.537 IS units 1432.0 576.3 unbinned pixels; need stage -0.242 0.656 if reset Tilting to 44 Correcting tilt backlash -9.9 -6.1 unbinned pixels; 0.9 4.0 nm along two shift axes; 2.5 -3.3 nm on specimen axes Rate = 20.46816 A/sec -4.4 3.4 unbinned pixels; 1.7 1.0 nm along two shift axes; -1.1 -1.6 nm on specimen axes Rate = 9.77017 A/sec Drift is low enough after shot 2 Measured defocus = -4.27 microns changed by 0.02 to target drift = 0.13 nm/sec Measured defocus = -4.25 microns changed by 0.00 to target drift = 0.11 nm/sec Defocus = -3.377 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.18.36_batch_tomo_11_21_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 113.8 In SetExposureForMean 120, exposure time changed to 2.7600 Image shift 0.028 -0.655 IS units 1801.9 546.4 unbinned pixels; need stage -0.243 0.861 if reset Tilting to -64 -64.00 degrees 4.2 -5.8 unbinned pixels; 2.4 0.7 nm along two shift axes; 1.9 1.6 nm on specimen axes Rate = 12.57569 A/sec Drift is low enough after shot 1 Measured defocus = -4.32 microns changed by 0.07 to target drift = 0.07 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -0.425 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.19.42_batch_tomo_11_21_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 30.3 In SetExposureForMean 120, exposure time changed to 21.2400 Trimming A by 25 25, reference by 105 115 Image shift 0.672 -2.069 IS units 6268.0 140.0 unbinned pixels; need stage -0.241 5.045 if reset Tilting to -66 Trimming A by 80 80, reference by 20 20 Trimming A by 70 70, reference by 20 20 0.5 -0.3 unbinned pixels; 0.2 0.1 nm along two shift axes; 0.1 0.2 nm on specimen axes Rate = 1.06765 A/sec Drift is low enough after shot 1 Measured defocus = -3.27 microns changed by -0.98 to target drift = 0.08 nm/sec Measured defocus = -4.18 microns changed by -0.07 to target drift = 0.05 nm/sec Defocus = -1.481 um Dewars are NOT busy filling 177 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.20.42_batch_tomo_11_21_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 126.2 In SetExposureForMean 120, exposure time changed to 107.1600 Image shift 1.001 -2.768 IS units 8486.0 -91.1 unbinned pixels; need stage -0.230 7.385 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -15.6 2.3 unbinned pixels; 2.6 4.9 nm along two shift axes; -0.3 -5.5 nm on specimen axes Rate = 27.57668 A/sec -12.3 2.9 unbinned pixels; 2.4 3.7 nm along two shift axes; -0.7 -4.4 nm on specimen axes Rate = 22.08037 A/sec -8.6 2.4 unbinned pixels; 1.8 2.6 nm along two shift axes; -0.6 -3.1 nm on specimen axes Rate = 15.66267 A/sec -7.1 2.2 unbinned pixels; 1.5 2.1 nm along two shift axes; -0.5 -2.6 nm on specimen axes Rate = 12.98166 A/sec Drift is low enough after shot 4 Measured defocus = -4.16 microns changed by -0.09 to target drift = 0.19 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.13 nm/sec Defocus = -3.458 um Dewars are NOT busy filling 893 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.22.25_batch_tomo_11_21_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 4358.7 In SetExposureForMean 120, exposure time changed to 3.0000 Image shift 0.018 -0.624 IS units 1709.7 544.8 unbinned pixels; need stage -0.240 0.845 if reset Tilting to 48 Correcting tilt backlash -8.2 -6.1 unbinned pixels; 1.1 3.5 nm along two shift axes; 2.4 -2.7 nm on specimen axes Rate = 18.06974 A/sec -4.4 2.9 unbinned pixels; 1.5 1.1 nm along two shift axes; -0.9 -1.6 nm on specimen axes Rate = 9.22645 A/sec Drift is low enough after shot 2 Measured defocus = -4.29 microns changed by 0.04 to target drift = 0.13 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -3.412 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.26.12_batch_tomo_11_21_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 113.7 In SetExposureForMean 120, exposure time changed to 3.2400 Image shift 0.059 -0.723 IS units 2018.0 527.2 unbinned pixels; need stage -0.244 1.041 if reset Tilting to -68 -68.00 degrees Trimming A by 110 110, reference by 20 20 Trimming A by 100 105, reference by 20 20 9.8 -0.7 unbinned pixels; 1.4 3.2 nm along two shift axes; -0.1 3.5 nm on specimen axes Rate = 17.21146 A/sec Trimming A by 100 100, reference by 20 20 23.1 -4.1 unbinned pixels; 4.1 7.2 nm along two shift axes; 0.7 8.2 nm on specimen axes Rate = 41.15085 A/sec Trimming A by 100 100, reference by 20 20 -0.3 -1.9 unbinned pixels; 0.6 0.3 nm along two shift axes; 0.7 -0.1 nm on specimen axes Rate = 3.43854 A/sec Drift is low enough after shot 3 Measured defocus = -3.54 microns changed by -0.71 to target drift = 0.23 nm/sec Measured defocus = -4.26 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -2.180 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.27.30_batch_tomo_11_21_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 15.3 mean counts (15.289122) below min Stopped negative side because counts below min Image shift 1.342 -3.476 IS units 10739.3 -345.1 unbinned pixels; need stage -0.212 10.167 if reset Omitting tilt at 50 Finishing Setting Eucentric Focus! Measured defocus = 0.25 microns changed by -4.50 to target drift = 0.64 nm/sec Focus area changed from stored state, axis position 2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_12.st Running script 1 at item 23 (-472.66, -186.91) 05:28:30 Refine ZLP: additional offset = 0.2 eV; total offset = -14.5 eV Item 23: Stage: -472.66 -186.91 -223 Label: 23 Note: Sec 22 - anchor_maps.map Target defocus is: -4.25 um Target defocus is: -4.50 um Target defocus = -4.5 Trimming A by 20 105, reference by 20 105 Angle = -4.40, increment = 0.60, shift = -31.5 -8.6 pixels, -0.074 um Y Trimming A by 21 110, reference by 20 105 Angle = -3.20, increment = 1.80, shift = -103.7 -22.6 pixels, -0.241 um Y Trimming A by 26 110, reference by 20 105 Angle = 0.41, increment = 5.41, shift = -324.3 -56.1 pixels, -0.750 um Y Trimming A by 37 115, reference by 20 105 Angle = 3.00, increment = 8.00, shift = -458.3 -77.9 pixels, -1.060 um Y Rough eucentricity: changing Z by 7.60 to -215.78, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 40 105, reference by 36 110 Angle = 18.00, shift = -51.3, -0.6 pixels, 0.012, -0.117 um, IS = -0.012, 0.117 Trimming A by 27 110, reference by 36 110 Angle = 11.00, shift = -18.9, 0.8 pixels, 0.007, -0.043 um, IS = -0.018, 0.159 Trimming A by 26 110, reference by 26 110 Angle = 4.00, shift = -46.2, -1.1 pixels, 0.009, -0.105 um, IS = -0.028, 0.265 Trimming A by 21 24 115 20, reference by 20 24 110 20 Angle = -4.00, shift = -44.6, -25.4 pixels, -0.046, -0.108 um, IS = 0.019, 0.372 Trimming A by 21 110, reference by 24 115 Angle = -11.00, shift = 25.4, -2.5 pixels, -0.012, 0.057 um, IS = 0.031, 0.315 Trimming A by 21 110, reference by 21 110 Angle = -18.00, shift = 51.7, 13.3 pixels, 0.017, 0.121 um, IS = 0.014, 0.195 Trimming A by 21 110, reference by 22 110 Angle = -24.00, shift = 49.0, 12.5 pixels, 0.016, 0.114 um, IS = -0.002, 0.080 General solution delZ 0.16, delY 2.88, yZero 0.31 solution with assumed YZero 0.32: delZ 0.16, delY 2.96 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.16 microns and lateral displacement of -2.88 microns Trimming A by 21 23 105 20, reference by 21 20 85 20 Disparity in stage position after aligning to center of map frame: -0.00 -1.74 Trimming A by 21 20 85 20, reference by 21 20 85 20 Disparity in stage position after aligning to target position: 0.41 -2.19 (2nd move error 0.04 0.08) Disparity in stage position after aligning to map item itself: 0.02 -1.65 (align shift -0.03 -0.03) Finished aligning to item in 3 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -472.65 -188.61 -215.93 -2.4 -0.1 unbinned pixels; 0.2 0.8 nm along two shift axes; 0.1 -0.8 nm on specimen axes Rate = 4.14619 A/sec Drift is low enough after shot 1 Measured defocus = 1.58 microns changed by -6.08 to target drift = 0.03 nm/sec Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.03 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -2.120 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.33.35_batch_tomo_12_23_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 77.5 In SetExposureForMean 120, exposure time changed to 1.8000 Image shift 0.030 -0.036 IS units 128.0 -46.5 unbinned pixels; need stage 0.012 0.047 if reset Tilting to -8 Correcting tilt backlash 8.9 -2.5 unbinned pixels; 1.9 2.6 nm along two shift axes; 0.6 3.2 nm on specimen axes Rate = 16.11817 A/sec 5.9 -0.4 unbinned pixels; 0.8 1.9 nm along two shift axes; -0.1 2.1 nm on specimen axes Rate = 10.31385 A/sec Drift is low enough after shot 2 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.08 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.04 nm/sec Defocus = -2.198 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.34.40_batch_tomo_12_23_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 116.2 In SetExposureForMean 120, change by a factor of 1.0329 would require too small a change in exposure time Image shift 0.003 -0.037 IS units 103.2 27.7 unbinned pixels; need stage -0.013 0.036 if reset Tilting to -12 -12.00 degrees -9.7 0.1 unbinned pixels; 1.2 3.2 nm along two shift axes; 0.3 -3.4 nm on specimen axes Rate = 17.05455 A/sec -3.9 2.6 unbinned pixels; 1.3 1.0 nm along two shift axes; -0.8 -1.5 nm on specimen axes Rate = 8.24058 A/sec Drift is low enough after shot 2 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -2.045 um Dewars are NOT busy filling 15 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.35.50_batch_tomo_12_23_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, exposure time changed to 1.9200 Image shift -0.037 0.035 IS units -131.5 68.6 unbinned pixels; need stage -0.020 -0.050 if reset Tilting to -14 -1.3 -1.5 unbinned pixels; 0.4 0.6 nm along two shift axes; 0.6 -0.4 nm on specimen axes Rate = 3.46127 A/sec Drift is low enough after shot 1 Measured defocus = -4.63 microns changed by 0.13 to target drift = 0.06 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -1.892 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.36.47_batch_tomo_12_23_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 123.8 In SetExposureForMean 120, change by a factor of 0.9700 would require too small a change in exposure time Image shift -0.071 0.111 IS units -370.0 87.9 unbinned pixels; need stage -0.019 -0.138 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 8.9 -1.6 unbinned pixels; 1.6 2.7 nm along two shift axes; 0.3 3.2 nm on specimen axes Rate = 15.78919 A/sec 5.1 -1.7 unbinned pixels; 1.2 1.5 nm along two shift axes; 0.4 1.9 nm on specimen axes Rate = 9.48032 A/sec Drift is low enough after shot 2 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.14 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -2.249 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.37.54_batch_tomo_12_23_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 123.6 In SetExposureForMean 120, change by a factor of 0.9710 would require too small a change in exposure time Image shift 0.006 -0.035 IS units 100.7 16.5 unbinned pixels; need stage -0.009 0.035 if reset Tilting to -4 Correcting tilt backlash -5.5 -0.6 unbinned pixels; 0.5 1.9 nm along two shift axes; 0.4 -1.9 nm on specimen axes Rate = 9.67667 A/sec Drift is low enough after shot 1 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.06 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -2.330 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.38.55_batch_tomo_12_23_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 123.2 In SetExposureForMean 120, change by a factor of 0.9744 would require too small a change in exposure time Image shift 0.018 -0.052 IS units 158.7 1.5 unbinned pixels; need stage -0.005 0.056 if reset Tilting to -16 -16.00 degrees -1.6 -7.4 unbinned pixels; 2.3 1.4 nm along two shift axes; 2.6 -0.3 nm on specimen axes Rate = 13.39543 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.823 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.39.54_batch_tomo_12_23_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 123.4 In SetExposureForMean 120, change by a factor of 0.9728 would require too small a change in exposure time Image shift -0.032 0.020 IS units -85.6 66.8 unbinned pixels; need stage -0.020 -0.034 if reset Tilting to -18 7.0 -2.3 unbinned pixels; 1.6 2.0 nm along two shift axes; 0.6 2.5 nm on specimen axes Rate = 12.94381 A/sec Drift is low enough after shot 1 Measured defocus = -4.60 microns changed by 0.10 to target drift = 0.14 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -1.716 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.40.53_batch_tomo_12_23_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 122.6 In SetExposureForMean 120, change by a factor of 0.9794 would require too small a change in exposure time Image shift -0.010 -0.006 IS units 5.5 34.1 unbinned pixels; need stage -0.012 0.001 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 5.6 -0.1 unbinned pixels; 0.7 1.9 nm along two shift axes; -0.1 2.0 nm on specimen axes Rate = 9.8824 A/sec Drift is low enough after shot 1 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.08 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.04 nm/sec Defocus = -2.386 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.41.55_batch_tomo_12_23_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 122.4 In SetExposureForMean 120, change by a factor of 0.9802 would require too small a change in exposure time Image shift 0.007 -0.027 IS units 79.0 7.2 unbinned pixels; need stage -0.005 0.028 if reset Tilting to 0 Correcting tilt backlash -4.4 -4.1 unbinned pixels; 0.8 2.0 nm along two shift axes; 1.6 -1.4 nm on specimen axes Rate = 10.62771 A/sec Drift is low enough after shot 1 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.04 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.02 nm/sec Defocus = -2.508 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.42.55_batch_tomo_12_23_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 122.1 In SetExposureForMean 120, change by a factor of 0.9826 would require too small a change in exposure time Image shift 0.031 -0.065 IS units 207.6 -23.6 unbinned pixels; need stage 0.002 0.074 if reset Tilting to -20 -20.00 degrees -2.1 -6.0 unbinned pixels; 1.8 1.4 nm along two shift axes; 2.2 -0.5 nm on specimen axes Rate = 11.28203 A/sec Drift is low enough after shot 1 Measured defocus = -4.61 microns changed by 0.11 to target drift = 0.03 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.01 nm/sec Defocus = -1.638 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.43.55_batch_tomo_12_23_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 122.2 In SetExposureForMean 120, change by a factor of 0.9821 would require too small a change in exposure time Image shift 0.015 -0.078 IS units 227.1 33.6 unbinned pixels; need stage -0.019 0.084 if reset Tilting to -22 7.0 -1.2 unbinned pixels; 1.2 2.2 nm along two shift axes; 0.2 2.5 nm on specimen axes Rate = 12.44573 A/sec Drift is low enough after shot 1 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.07 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.09 nm/sec Defocus = -1.521 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.44.52_batch_tomo_12_23_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 121.4 In SetExposureForMean 120, change by a factor of 0.9888 would require too small a change in exposure time Image shift 0.024 -0.078 IS units 235.0 9.3 unbinned pixels; need stage -0.010 0.089 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees 4.3 -1.1 unbinned pixels; 0.9 1.3 nm along two shift axes; 0.2 1.5 nm on specimen axes Rate = 7.69709 A/sec Drift is low enough after shot 1 Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -2.490 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.45.55_batch_tomo_12_23_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 121.4 In SetExposureForMean 120, change by a factor of 0.9885 would require too small a change in exposure time Image shift 0.004 -0.037 IS units 103.5 24.2 unbinned pixels; need stage -0.012 0.036 if reset Tilting to 4 Correcting tilt backlash -1.4 -6.0 unbinned pixels; 1.8 1.2 nm along two shift axes; 2.2 -0.3 nm on specimen axes Rate = 10.94067 A/sec Drift is low enough after shot 1 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.05 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -2.534 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.46.55_batch_tomo_12_23_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 120.4 In SetExposureForMean 120, change by a factor of 0.9971 would require too small a change in exposure time Image shift 0.053 -0.127 IS units 395.6 -23.9 unbinned pixels; need stage -0.004 0.141 if reset Tilting to -24 -24.00 degrees -2.6 -5.8 unbinned pixels; 1.6 1.6 nm along two shift axes; 2.1 -0.7 nm on specimen axes Rate = 11.32839 A/sec Drift is low enough after shot 1 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.476 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.47.57_batch_tomo_12_23_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 120.3 In SetExposureForMean 120, change by a factor of 0.9980 would require too small a change in exposure time Image shift 0.043 -0.152 IS units 454.4 26.3 unbinned pixels; need stage -0.023 0.175 if reset Tilting to -26 5.9 -6.4 unbinned pixels; 2.8 1.2 nm along two shift axes; 2.1 2.2 nm on specimen axes Rate = 15.19793 A/sec 6.7 1.5 unbinned pixels; 0.3 2.4 nm along two shift axes; -0.7 2.3 nm on specimen axes Rate = 12.01885 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.06 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -1.421 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.49.01_batch_tomo_12_23_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 119.0 In SetExposureForMean 120, change by a factor of 1.0084 would require too small a change in exposure time Image shift 0.070 -0.189 IS units 581.9 -11.4 unbinned pixels; need stage -0.014 0.229 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees -3.1 2.2 unbinned pixels; 1.1 0.7 nm along two shift axes; -0.7 -1.1 nm on specimen axes Rate = 6.65285 A/sec Drift is low enough after shot 1 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.07 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.06 nm/sec Defocus = -2.533 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.50.05_batch_tomo_12_23_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 119.0 In SetExposureForMean 120, change by a factor of 1.0086 would require too small a change in exposure time Image shift 0.033 -0.120 IS units 358.0 25.1 unbinned pixels; need stage -0.020 0.126 if reset Tilting to 8 Correcting tilt backlash 2.7 -5.2 unbinned pixels; 2.0 0.3 nm along two shift axes; 1.7 1.1 nm on specimen axes Rate = 10.31668 A/sec Drift is low enough after shot 1 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.11 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.03 nm/sec Defocus = -2.597 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.51.05_batch_tomo_12_23_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0217 would require too small a change in exposure time Image shift 0.070 -0.180 IS units 557.6 -18.2 unbinned pixels; need stage -0.011 0.200 if reset Tilting to -28 -28.00 degrees -3.4 -2.8 unbinned pixels; 0.5 1.5 nm along two shift axes; 1.1 -1.1 nm on specimen axes Rate = 7.78327 A/sec Drift is low enough after shot 1 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.10 nm/sec Defocus = -1.432 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.52.07_batch_tomo_12_23_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 117.6 In SetExposureForMean 120, change by a factor of 1.0213 would require too small a change in exposure time Image shift 0.088 -0.264 IS units 800.6 11.4 unbinned pixels; need stage -0.029 0.320 if reset Tilting to -30 6.6 -2.1 unbinned pixels; 1.5 1.9 nm along two shift axes; 0.5 2.4 nm on specimen axes Rate = 12.1526 A/sec Drift is low enough after shot 1 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.09 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.07 nm/sec Defocus = -1.369 um Dewars are NOT busy filling 16 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.53.05_batch_tomo_12_23_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, exposure time changed to 2.0400 Image shift 0.106 -0.282 IS units 867.3 -20.5 unbinned pixels; need stage -0.020 0.355 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -22.6 6.6 unbinned pixels; 4.9 6.7 nm along two shift axes; -1.6 -8.1 nm on specimen axes Rate = 41.36122 A/sec -14.2 2.5 unbinned pixels; 2.5 4.4 nm along two shift axes; -0.4 -5.1 nm on specimen axes Rate = 25.33028 A/sec -5.7 2.4 unbinned pixels; 1.5 1.6 nm along two shift axes; -0.7 -2.1 nm on specimen axes Rate = 10.84156 A/sec Drift is low enough after shot 3 Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.17 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.13 nm/sec Defocus = -2.666 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.54.21_batch_tomo_12_23_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 123.2 In SetExposureForMean 120, change by a factor of 0.9743 would require too small a change in exposure time Image shift 0.038 -0.149 IS units 440.2 38.4 unbinned pixels; need stage -0.027 0.157 if reset Tilting to 12 Correcting tilt backlash 16.3 -5.2 unbinned pixels; 3.6 4.8 nm along two shift axes; 1.3 5.9 nm on specimen axes Rate = 30.03198 A/sec 13.4 5.2 unbinned pixels; 0.2 5.0 nm along two shift axes; -2.2 4.5 nm on specimen axes Rate = 25.23295 A/sec 6.3 1.7 unbinned pixels; 0.2 2.3 nm along two shift axes; -0.8 2.1 nm on specimen axes Rate = 11.41299 A/sec Drift is low enough after shot 3 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.08 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -2.685 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.55.33_batch_tomo_12_23_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 121.4 In SetExposureForMean 120, change by a factor of 0.9889 would require too small a change in exposure time Image shift 0.079 -0.234 IS units 711.7 8.4 unbinned pixels; need stage -0.025 0.257 if reset Tilting to -32 -32.00 degrees -0.7 -5.7 unbinned pixels; 1.8 0.9 nm along two shift axes; 2.0 -0.1 nm on specimen axes Rate = 10.0683 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.06 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.01 nm/sec Defocus = -1.336 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.56.35_batch_tomo_12_23_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 121.3 In SetExposureForMean 120, change by a factor of 0.9901 would require too small a change in exposure time Image shift 0.125 -0.359 IS units 1095.7 0.4 unbinned pixels; need stage -0.034 0.457 if reset Tilting to -34 4.3 -6.1 unbinned pixels; 2.5 0.7 nm along two shift axes; 2.0 1.7 nm on specimen axes Rate = 13.08135 A/sec Drift is low enough after shot 1 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.11 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -1.298 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.57.33_batch_tomo_12_23_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 119.2 In SetExposureForMean 120, change by a factor of 1.0080 would require too small a change in exposure time Image shift 0.154 -0.397 IS units 1226.3 -40.4 unbinned pixels; need stage -0.024 0.525 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -40.1 7.0 unbinned pixels; 7.0 12.4 nm along two shift axes; -1.2 -14.3 nm on specimen axes Rate = 71.34418 A/sec -14.8 6.6 unbinned pixels; 3.9 4.1 nm along two shift axes; -1.9 -5.4 nm on specimen axes Rate = 28.35923 A/sec -8.6 1.8 unbinned pixels; 1.6 2.6 nm along two shift axes; -0.4 -3.1 nm on specimen axes Rate = 15.44838 A/sec -6.2 1.7 unbinned pixels; 1.3 1.9 nm along two shift axes; -0.4 -2.2 nm on specimen axes Rate = 11.31232 A/sec Drift is low enough after shot 4 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.12 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -2.789 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_05.58.55_batch_tomo_12_23_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 119.1 In SetExposureForMean 120, change by a factor of 1.0079 would require too small a change in exposure time Image shift 0.024 -0.130 IS units 376.3 57.7 unbinned pixels; need stage -0.031 0.135 if reset Tilting to 16 Correcting tilt backlash 23.6 -3.7 unbinned pixels; 4.0 7.4 nm along two shift axes; 0.6 8.4 nm on specimen axes Rate = 41.93858 A/sec 14.1 5.2 unbinned pixels; 0.1 5.3 nm along two shift axes; -2.2 4.8 nm on specimen axes Rate = 26.50353 A/sec 6.9 1.9 unbinned pixels; 0.2 2.5 nm along two shift axes; -0.9 2.3 nm on specimen axes Rate = 12.49431 A/sec Drift is low enough after shot 3 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.10 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -2.833 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.00.07_batch_tomo_12_23_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 116.9 In SetExposureForMean 120, change by a factor of 1.0267 would require too small a change in exposure time Image shift 0.073 -0.235 IS units 707.8 25.5 unbinned pixels; need stage -0.031 0.259 if reset Tilting to -36 -36.00 degrees 2.0 -1.8 unbinned pixels; 0.8 0.4 nm along two shift axes; 0.6 0.7 nm on specimen axes Rate = 4.69243 A/sec Drift is low enough after shot 1 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.06 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -1.246 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.01.10_batch_tomo_12_23_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 117.1 In SetExposureForMean 120, change by a factor of 1.0258 would require too small a change in exposure time Image shift 0.167 -0.446 IS units 1374.3 -30.9 unbinned pixels; need stage -0.032 0.602 if reset Tilting to -38 4.0 -0.3 unbinned pixels; 0.6 1.3 nm along two shift axes; -0.0 1.4 nm on specimen axes Rate = 7.02583 A/sec Drift is low enough after shot 1 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.10 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = -1.214 um Dewars are NOT busy filling 17 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.02.08_batch_tomo_12_23_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 114.5 In SetExposureForMean 120, exposure time changed to 2.1600 Image shift 0.170 -0.440 IS units 1360.3 -42.6 unbinned pixels; need stage -0.027 0.612 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -27.3 6.6 unbinned pixels; 5.4 8.2 nm along two shift axes; -1.5 -9.8 nm on specimen axes Rate = 49.13476 A/sec -6.7 3.1 unbinned pixels; 1.8 1.9 nm along two shift axes; -0.9 -2.5 nm on specimen axes Rate = 12.9662 A/sec Drift is low enough after shot 2 Measured defocus = -4.38 microns changed by -0.12 to target drift = 0.13 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.09 nm/sec Defocus = -2.936 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.03.19_batch_tomo_12_23_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 121.2 In SetExposureForMean 120, change by a factor of 0.9906 would require too small a change in exposure time Image shift 0.042 -0.178 IS units 524.3 54.1 unbinned pixels; need stage -0.035 0.193 if reset Tilting to 20 Correcting tilt backlash 13.9 -1.8 unbinned pixels; 2.2 4.4 nm along two shift axes; 0.2 4.9 nm on specimen axes Rate = 24.55948 A/sec 3.4 6.0 unbinned pixels; 1.6 1.9 nm along two shift axes; -2.2 1.0 nm on specimen axes Rate = 12.25689 A/sec Drift is low enough after shot 2 Measured defocus = -4.44 microns changed by -0.06 to target drift = 0.06 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.04 nm/sec Defocus = -2.971 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.04.25_batch_tomo_12_23_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 118.2 In SetExposureForMean 120, change by a factor of 1.0157 would require too small a change in exposure time Image shift 0.099 -0.317 IS units 955.4 30.8 unbinned pixels; need stage -0.040 0.358 if reset Tilting to -40 -40.00 degrees 3.1 -1.4 unbinned pixels; 0.8 0.9 nm along two shift axes; 0.4 1.1 nm on specimen axes Rate = 5.97915 A/sec Drift is low enough after shot 1 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.09 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -1.171 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.05.28_batch_tomo_12_23_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 118.5 In SetExposureForMean 120, change by a factor of 1.0135 would require too small a change in exposure time Image shift 0.179 -0.478 IS units 1471.1 -31.6 unbinned pixels; need stage -0.034 0.680 if reset Tilting to -42 2.2 -2.2 unbinned pixels; 1.0 0.5 nm along two shift axes; 0.7 0.9 nm on specimen axes Rate = 5.47163 A/sec Drift is low enough after shot 1 Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.14 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.09 nm/sec Defocus = -1.161 um Dewars are NOT busy filling 18 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.06.26_batch_tomo_12_23_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 115.2 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.198 -0.511 IS units 1577.9 -51.9 unbinned pixels; need stage -0.031 0.753 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -12.9 5.1 unbinned pixels; 3.2 3.7 nm along two shift axes; -1.4 -4.7 nm on specimen axes Rate = 24.2837 A/sec -4.0 2.8 unbinned pixels; 1.4 1.0 nm along two shift axes; -0.8 -1.5 nm on specimen axes Rate = 8.58514 A/sec Drift is low enough after shot 2 Measured defocus = -4.36 microns changed by -0.14 to target drift = 0.10 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.09 nm/sec Defocus = -3.105 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.07.37_batch_tomo_12_23_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 121.5 In SetExposureForMean 120, change by a factor of 0.9881 would require too small a change in exposure time Image shift 0.074 -0.269 IS units 801.2 54.0 unbinned pixels; need stage -0.043 0.304 if reset Tilting to 24 Correcting tilt backlash 4.5 -5.1 unbinned pixels; 2.2 0.9 nm along two shift axes; 1.7 1.7 nm on specimen axes Rate = 11.99879 A/sec Drift is low enough after shot 1 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.12 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -3.140 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.08.38_batch_tomo_12_23_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 118.2 In SetExposureForMean 120, change by a factor of 1.0163 would require too small a change in exposure time Image shift 0.144 -0.437 IS units 1325.4 23.8 unbinned pixels; need stage -0.049 0.512 if reset Tilting to -44 -44.00 degrees 5.0 -2.5 unbinned pixels; 1.4 1.4 nm along two shift axes; 0.7 1.8 nm on specimen axes Rate = 9.8548 A/sec Drift is low enough after shot 1 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.12 nm/sec Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.10 nm/sec Defocus = -1.059 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.09.42_batch_tomo_12_23_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 118.2 In SetExposureForMean 120, change by a factor of 1.0162 would require too small a change in exposure time Image shift 0.214 -0.562 IS units 1732.2 -48.1 unbinned pixels; need stage -0.037 0.854 if reset Tilting to -46 2.8 -4.8 unbinned pixels; 1.9 0.4 nm along two shift axes; 1.6 1.1 nm on specimen axes Rate = 9.73923 A/sec Drift is low enough after shot 1 Measured defocus = -4.45 microns changed by -0.05 to target drift = 0.09 nm/sec Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.04 nm/sec Defocus = -1.075 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.10.41_batch_tomo_12_23_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 114.4 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.242 -0.609 IS units 1887.5 -80.8 unbinned pixels; need stage -0.030 0.965 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -11.6 3.4 unbinned pixels; 2.5 3.4 nm along two shift axes; -0.8 -4.2 nm on specimen axes Rate = 21.22311 A/sec -6.9 5.2 unbinned pixels; 2.5 1.7 nm along two shift axes; -1.6 -2.6 nm on specimen axes Rate = 15.14752 A/sec -3.8 1.7 unbinned pixels; 1.0 1.1 nm along two shift axes; -0.5 -1.4 nm on specimen axes Rate = 7.23819 A/sec Drift is low enough after shot 3 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.10 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.15 nm/sec Defocus = -3.250 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.11.59_batch_tomo_12_23_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 120.2 In SetExposureForMean 120, change by a factor of 0.9993 would require too small a change in exposure time Image shift 0.108 -0.365 IS units 1096.2 53.7 unbinned pixels; need stage -0.052 0.429 if reset Tilting to 28 Correcting tilt backlash -4.2 -2.7 unbinned pixels; 0.4 1.7 nm along two shift axes; 1.1 -1.4 nm on specimen axes Rate = 8.85448 A/sec Drift is low enough after shot 1 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.14 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.08 nm/sec Defocus = -3.281 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.13.00_batch_tomo_12_23_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 116.0 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift 0.169 -0.505 IS units 1534.1 19.1 unbinned pixels; need stage -0.054 0.614 if reset Tilting to -48 -48.00 degrees 5.1 -3.3 unbinned pixels; 1.7 1.3 nm along two shift axes; 1.0 1.9 nm on specimen axes Rate = 10.55348 A/sec Drift is low enough after shot 1 Measured defocus = -4.59 microns changed by 0.09 to target drift = 0.13 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -0.981 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.14.04_batch_tomo_12_23_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 121.8 In SetExposureForMean 120, change by a factor of 0.9862 would require too small a change in exposure time Image shift 0.261 -0.655 IS units 2031.2 -87.1 unbinned pixels; need stage -0.033 1.078 if reset Tilting to -50 3.6 -5.0 unbinned pixels; 2.1 0.6 nm along two shift axes; 1.6 1.4 nm on specimen axes Rate = 10.8342 A/sec Drift is low enough after shot 1 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.05 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.05 nm/sec Defocus = -1.024 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.15.03_batch_tomo_12_23_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 117.1 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.289 -0.708 IS units 2201.7 -112.1 unbinned pixels; need stage -0.029 1.217 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -12.7 4.8 unbinned pixels; 3.1 3.6 nm along two shift axes; -1.3 -4.6 nm on specimen axes Rate = 23.80386 A/sec -6.4 3.1 unbinned pixels; 1.8 1.7 nm along two shift axes; -0.9 -2.3 nm on specimen axes Rate = 12.49464 A/sec Drift is low enough after shot 2 Measured defocus = -4.40 microns changed by -0.10 to target drift = 0.19 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.15 nm/sec Defocus = -3.391 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.16.21_batch_tomo_12_23_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 122.5 In SetExposureForMean 120, change by a factor of 0.9815 would require too small a change in exposure time Image shift 0.142 -0.446 IS units 1347.5 36.7 unbinned pixels; need stage -0.054 0.549 if reset Tilting to 32 Correcting tilt backlash -11.7 -5.1 unbinned pixels; 0.3 4.5 nm along two shift axes; 2.1 -4.0 nm on specimen axes Rate = 22.55565 A/sec -5.1 3.4 unbinned pixels; 1.7 1.3 nm along two shift axes; -1.0 -1.9 nm on specimen axes Rate = 10.78183 A/sec Drift is low enough after shot 2 Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.09 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.09 nm/sec Defocus = -3.396 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.17.28_batch_tomo_12_23_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 117.0 In SetExposureForMean 120, exposure time changed to 2.7600 Image shift 0.205 -0.576 IS units 1762.4 -9.1 unbinned pixels; need stage -0.051 0.735 if reset Tilting to -52 -52.00 degrees 7.6 -8.7 unbinned pixels; 3.8 1.5 nm along two shift axes; 2.8 2.9 nm on specimen axes Rate = 20.3174 A/sec 5.7 2.2 unbinned pixels; 0.1 2.2 nm along two shift axes; -0.9 1.9 nm on specimen axes Rate = 10.81968 A/sec Drift is low enough after shot 2 Measured defocus = -4.58 microns changed by 0.08 to target drift = 0.09 nm/sec Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -0.929 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.18.39_batch_tomo_12_23_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 122.8 In SetExposureForMean 120, change by a factor of 0.9789 would require too small a change in exposure time Image shift 0.334 -0.785 IS units 2455.5 -161.2 unbinned pixels; need stage -0.020 1.419 if reset Tilting to -54 3.3 -5.0 unbinned pixels; 2.0 0.5 nm along two shift axes; 1.6 1.3 nm on specimen axes Rate = 10.46563 A/sec Drift is low enough after shot 1 Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.05 nm/sec Measured defocus = -4.53 microns changed by 0.03 to target drift = 0.13 nm/sec Defocus = -0.936 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.19.43_batch_tomo_12_23_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 116.9 In SetExposureForMean 120, exposure time changed to 2.8800 Image shift 0.355 -0.836 IS units 2615.7 -170.2 unbinned pixels; need stage -0.022 1.583 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -18.0 2.9 unbinned pixels; 3.1 5.6 nm along two shift axes; -0.5 -6.4 nm on specimen axes Rate = 32.04603 A/sec -9.3 3.1 unbinned pixels; 2.1 2.7 nm along two shift axes; -0.8 -3.4 nm on specimen axes Rate = 17.25216 A/sec -8.1 2.4 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.6 -2.9 nm on specimen axes Rate = 14.81378 A/sec -7.7 1.6 unbinned pixels; 1.4 2.4 nm along two shift axes; -0.3 -2.7 nm on specimen axes Rate = 13.72918 A/sec Drift is low enough after shot 4 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.14 nm/sec Measured defocus = -4.46 microns changed by -0.04 to target drift = 0.14 nm/sec Defocus = -3.511 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.21.07_batch_tomo_12_23_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 121.9 In SetExposureForMean 120, change by a factor of 0.9869 would require too small a change in exposure time Image shift 0.195 -0.539 IS units 1653.2 -16.2 unbinned pixels; need stage -0.045 0.706 if reset Tilting to 36 Correcting tilt backlash -12.0 -8.1 unbinned pixels; 1.3 4.9 nm along two shift axes; 3.2 -4.0 nm on specimen axes Rate = 25.56576 A/sec -3.2 3.4 unbinned pixels; 1.5 0.6 nm along two shift axes; -1.1 -1.2 nm on specimen axes Rate = 8.1689 A/sec Drift is low enough after shot 2 Measured defocus = -4.54 microns changed by 0.04 to target drift = 0.10 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.11 nm/sec Defocus = -3.484 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.22.14_batch_tomo_12_23_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 115.4 In SetExposureForMean 120, exposure time changed to 3.0000 Image shift 0.265 -0.684 IS units 2114.3 -68.9 unbinned pixels; need stage -0.041 0.927 if reset Tilting to -56 -56.00 degrees 7.7 -6.2 unbinned pixels; 3.0 1.8 nm along two shift axes; 1.9 2.9 nm on specimen axes Rate = 17.35331 A/sec 6.2 1.8 unbinned pixels; 0.1 2.3 nm along two shift axes; -0.8 2.1 nm on specimen axes Rate = 11.31423 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.10 nm/sec Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.08 nm/sec Defocus = -0.818 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.23.30_batch_tomo_12_23_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 120.8 In SetExposureForMean 120, change by a factor of 0.9956 would require too small a change in exposure time Image shift 0.382 -0.876 IS units 2749.7 -207.5 unbinned pixels; need stage -0.013 1.751 if reset Tilting to -58 1.2 -5.2 unbinned pixels; 1.8 0.2 nm along two shift axes; 1.8 0.6 nm on specimen axes Rate = 9.3189 A/sec Drift is low enough after shot 1 Measured defocus = -4.43 microns changed by -0.07 to target drift = 0.12 nm/sec Measured defocus = -4.52 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -0.868 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.24.30_batch_tomo_12_23_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 113.6 In SetExposureForMean 120, exposure time changed to 3.1200 Image shift 0.408 -0.937 IS units 2940.2 -218.9 unbinned pixels; need stage -0.015 1.975 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -22.0 5.0 unbinned pixels; 4.2 6.7 nm along two shift axes; -1.1 -7.9 nm on specimen axes Rate = 39.61312 A/sec -14.6 2.8 unbinned pixels; 2.6 4.5 nm along two shift axes; -0.5 -5.2 nm on specimen axes Rate = 26.10908 A/sec -6.7 3.2 unbinned pixels; 1.9 1.8 nm along two shift axes; -0.9 -2.5 nm on specimen axes Rate = 13.06251 A/sec Drift is low enough after shot 3 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.24 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.18 nm/sec Defocus = -3.495 um Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.25.49_batch_tomo_12_23_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 118.1 In SetExposureForMean 120, exposure time changed to 3.2400 Image shift 0.255 -0.651 IS units 2016.0 -73.8 unbinned pixels; need stage -0.037 0.908 if reset Tilting to 40 Correcting tilt backlash -9.9 -5.7 unbinned pixels; 0.7 3.9 nm along two shift axes; 2.3 -3.3 nm on specimen axes Rate = 20.07249 A/sec -6.3 3.4 unbinned pixels; 1.9 1.7 nm along two shift axes; -1.0 -2.3 nm on specimen axes Rate = 12.56121 A/sec Drift is low enough after shot 2 Measured defocus = -4.51 microns changed by 0.01 to target drift = 0.19 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.11 nm/sec Defocus = -3.488 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.26.56_batch_tomo_12_23_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 114.7 In SetExposureForMean 120, exposure time changed to 3.3600 Image shift 0.294 -0.739 IS units 2292.9 -97.6 unbinned pixels; need stage -0.037 1.062 if reset Tilting to -60 -60.00 degrees 3.4 -7.3 unbinned pixels; 2.8 0.3 nm along two shift axes; 2.4 1.4 nm on specimen axes Rate = 14.12664 A/sec 6.5 1.9 unbinned pixels; 0.1 2.4 nm along two shift axes; -0.9 2.2 nm on specimen axes Rate = 11.81544 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.07 nm/sec Measured defocus = -4.47 microns changed by -0.03 to target drift = 0.04 nm/sec Defocus = -0.837 um Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.28.08_batch_tomo_12_23_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 119.2 In SetExposureForMean 120, change by a factor of 1.0075 would require too small a change in exposure time Image shift 0.445 -1.005 IS units 3159.8 -254.3 unbinned pixels; need stage -0.010 2.251 if reset Tilting to -62 -1.5 -0.9 unbinned pixels; 0.1 0.6 nm along two shift axes; 0.4 -0.5 nm on specimen axes Rate = 3.16192 A/sec Drift is low enough after shot 1 Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -0.828 um Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.29.08_batch_tomo_12_23_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 110.6 In SetExposureForMean 120, exposure time changed to 3.6000 Image shift 0.458 -1.054 IS units 3307.9 -243.4 unbinned pixels; need stage -0.018 2.508 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -15.7 3.3 unbinned pixels; 2.9 4.8 nm along two shift axes; -0.7 -5.6 nm on specimen axes Rate = 28.06875 A/sec -14.8 4.3 unbinned pixels; 3.1 4.4 nm along two shift axes; -1.0 -5.3 nm on specimen axes Rate = 26.97608 A/sec -6.6 2.5 unbinned pixels; 1.6 1.9 nm along two shift axes; -0.7 -2.4 nm on specimen axes Rate = 12.40316 A/sec Drift is low enough after shot 3 Measured defocus = -4.41 microns changed by -0.09 to target drift = 0.21 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.16 nm/sec Defocus = -3.581 um Dewars are NOT busy filling 30 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.30.28_batch_tomo_12_23_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 118.5 In SetExposureForMean 120, exposure time changed to 3.7200 Image shift 0.297 -0.747 IS units 2317.1 -98.0 unbinned pixels; need stage -0.038 1.107 if reset Tilting to 44 Correcting tilt backlash -8.2 -8.2 unbinned pixels; 1.8 3.7 nm along two shift axes; 3.1 -2.6 nm on specimen axes Rate = 20.45694 A/sec -4.9 4.8 unbinned pixels; 2.2 1.1 nm along two shift axes; -1.5 -1.9 nm on specimen axes Rate = 12.08424 A/sec Drift is low enough after shot 2 Measured defocus = -4.56 microns changed by 0.06 to target drift = 0.08 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.14 nm/sec Defocus = -3.530 um Dewars are NOT busy filling 31 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.31.35_batch_tomo_12_23_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 113.1 In SetExposureForMean 120, exposure time changed to 3.9600 Image shift 0.369 -0.877 IS units 2739.8 -169.2 unbinned pixels; need stage -0.026 1.354 if reset Tilting to -64 -64.00 degrees 2.7 -7.1 unbinned pixels; 2.7 0.0 nm along two shift axes; 2.4 1.2 nm on specimen axes Rate = 13.38978 A/sec Drift is low enough after shot 1 Measured defocus = -4.57 microns changed by 0.07 to target drift = 0.09 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.05 nm/sec Defocus = -0.779 um Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.32.43_batch_tomo_12_23_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 119.9 In SetExposureForMean 120, change by a factor of 1.0013 would require too small a change in exposure time Image shift 0.499 -1.119 IS units 3522.6 -293.9 unbinned pixels; need stage -0.007 2.863 if reset Tilting to -66 -1.8 -5.3 unbinned pixels; 1.6 1.2 nm along two shift axes; 1.9 -0.5 nm on specimen axes Rate = 9.94117 A/sec Drift is low enough after shot 1 Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.06 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -0.785 um Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.33.43_batch_tomo_12_23_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 109.3 In SetExposureForMean 120, exposure time changed to 4.3200 Image shift 0.526 -1.188 IS units 3737.6 -300.6 unbinned pixels; need stage -0.012 3.273 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -22.6 2.8 unbinned pixels; 3.6 7.1 nm along two shift axes; -0.3 -8.0 nm on specimen axes Rate = 39.91881 A/sec -12.1 3.1 unbinned pixels; 2.4 3.6 nm along two shift axes; -0.7 -4.3 nm on specimen axes Rate = 21.81185 A/sec -9.4 1.5 unbinned pixels; 1.6 2.9 nm along two shift axes; -0.2 -3.4 nm on specimen axes Rate = 16.75159 A/sec -6.2 2.0 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.2 nm on specimen axes Rate = 11.31311 A/sec Drift is low enough after shot 4 Measured defocus = -4.42 microns changed by -0.08 to target drift = 0.11 nm/sec Measured defocus = -4.50 microns changed by 0.00 to target drift = 0.14 nm/sec Defocus = -3.604 um Dewars are NOT busy filling 36 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.35.09_batch_tomo_12_23_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 119.8 In SetExposureForMean 120, change by a factor of 1.0122 would require too small a change in exposure time Image shift 0.359 -0.866 IS units 2699.9 -152.5 unbinned pixels; need stage -0.031 1.381 if reset Tilting to 48 Correcting tilt backlash -9.1 -5.2 unbinned pixels; 0.7 3.6 nm along two shift axes; 2.1 -3.0 nm on specimen axes Rate = 18.43622 A/sec -5.3 3.3 unbinned pixels; 1.7 1.4 nm along two shift axes; -1.0 -1.9 nm on specimen axes Rate = 10.85301 A/sec Drift is low enough after shot 2 Measured defocus = -4.50 microns changed by -0.00 to target drift = 0.18 nm/sec Measured defocus = -4.49 microns changed by -0.01 to target drift = 0.12 nm/sec Defocus = -3.617 um Dewars are NOT busy filling 36 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.36.19_batch_tomo_12_23_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 107.7 In SetExposureForMean 120, exposure time changed to 4.9200 Image shift 0.400 -0.954 IS units 2978.5 -180.5 unbinned pixels; need stage -0.030 1.583 if reset Tilting to -68 -68.00 degrees Trimming A by 161 175, reference by 20 20 0.8 -7.6 unbinned pixels; 2.6 0.6 nm along two shift axes; 2.7 0.5 nm on specimen axes Rate = 13.56713 A/sec Drift is low enough after shot 1 Measured defocus = -4.88 microns changed by 0.38 to target drift = 0.03 nm/sec Measured defocus = -4.48 microns changed by -0.02 to target drift = 0.01 nm/sec Defocus = -0.423 um Dewars are NOT busy filling 41 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.37.27_batch_tomo_12_23_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 122.0 In SetExposureForMean 120, exposure time changed to 4.8000 Image shift 0.577 -1.263 IS units 3988.9 -367.9 unbinned pixels; need stage 0.004 3.797 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 0.97 microns changed by -5.47 to target drift = 0.51 nm/sec Focus area changed from stored state, axis position -2.80 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_13.st Running script 1 at item 25 (-546.16, -257.52) 06:38:37 Refine ZLP: additional offset = -0.4 eV; total offset = -15.0 eV Item 25: Stage: -546.16 -257.52 -223 Label: 25 Note: Sec 24 - anchor_maps.map Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Target defocus is: -4.50 um Target defocus is: -3.50 um Target defocus = -3.5 Trimming A by 21 105, reference by 20 105 Angle = -4.40, increment = 0.60, shift = -29.3 -7.9 pixels, -0.068 um Y Trimming A by 21 105, reference by 20 105 Angle = -3.20, increment = 1.80, shift = -90.6 -15.4 pixels, -0.209 um Y Trimming A by 20 100, reference by 20 105 Angle = 0.40, increment = 5.41, shift = -297.3 -47.0 pixels, -0.687 um Y Trimming A by 21 100, reference by 20 105 Angle = 3.00, increment = 8.00, shift = -421.9 -65.1 pixels, -0.974 um Y Rough eucentricity: changing Z by 6.98 to -216.40, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 21 105, reference by 23 105 Angle = 18.00, shift = 28.4, 16.2 pixels, 0.029, 0.069 um, IS = -0.029, -0.069 Trimming A by 22 105, reference by 21 105 Angle = 11.00, shift = -32.8, 2.8 pixels, 0.015, -0.074 um, IS = -0.044, 0.005 Trimming A by 22 105, reference by 22 105 Angle = 4.00, shift = 15.5, 7.9 pixels, 0.014, 0.037 um, IS = -0.058, -0.032 Trimming A by 25 21 20 100, reference by 20 21 20 105 Angle = -4.00, shift = -5.2, -18.0 pixels, -0.040, -0.016 um, IS = -0.019, -0.016 Trimming A by 27 105, reference by 26 100 Angle = -11.00, shift = 10.9, -5.7 pixels, -0.016, 0.023 um, IS = -0.003, -0.039 Trimming A by 46 105, reference by 32 105 Angle = -18.00, shift = 32.0, 11.5 pixels, 0.018, 0.076 um, IS = -0.021, -0.115 Trimming A by 65 100, reference by 51 105 Angle = -24.00, shift = 46.0, 11.9 pixels, 0.015, 0.107 um, IS = -0.036, -0.222 General solution delZ -0.18, delY 1.21, yZero -0.01 solution with assumed YZero -0.02: delZ -0.18, delY 1.07 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of -0.18 microns and lateral displacement of -1.21 microns Trimming A by 21 24 20 115, reference by 21 20 35 110 Disparity in stage position after aligning to center of map frame: 0.12 -1.51 Trimming A by 21 20 65 115, reference by 21 20 35 110 Disparity in stage position after aligning to target position: 0.54 -1.95 (2nd move error 0.04 0.10) Disparity in stage position after aligning to map item itself: 0.15 -1.43 (align shift -0.03 -0.03) Finished aligning to item in 3 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -546.03 -259.02 -216.21 0.4 1.7 unbinned pixels; 0.5 0.3 nm along two shift axes; -0.6 0.1 nm on specimen axes Rate = 3.08433 A/sec Drift is low enough after shot 1 Measured defocus = 1.42 microns changed by -4.92 to target drift = 0.10 nm/sec Measured defocus = -3.39 microns changed by -0.11 to target drift = 0.08 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.06 nm/sec Defocus = -1.152 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.47.48_batch_tomo_13_25_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 64.4 In SetExposureForMean 120, exposure time changed to 2.2800 Image shift 0.038 -0.056 IS units 187.8 -49.8 unbinned pixels; need stage 0.011 0.069 if reset Tilting to -8 Correcting tilt backlash 9.4 0.3 unbinned pixels; 1.0 3.2 nm along two shift axes; -0.4 3.3 nm on specimen axes Rate = 16.51702 A/sec 7.0 2.3 unbinned pixels; 0.0 2.6 nm along two shift axes; -1.0 2.4 nm on specimen axes Rate = 13.05887 A/sec Drift is low enough after shot 2 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.11 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -1.234 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.48.53_batch_tomo_13_25_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 122.0 In SetExposureForMean 120, change by a factor of 0.9837 would require too small a change in exposure time Image shift -0.015 -0.052 IS units 127.1 89.6 unbinned pixels; need stage -0.035 0.042 if reset Tilting to -12 -12.00 degrees -8.3 7.3 unbinned pixels; 3.4 1.9 nm along two shift axes; -2.3 -3.1 nm on specimen axes Rate = 19.35393 A/sec -2.7 2.5 unbinned pixels; 1.1 0.6 nm along two shift axes; -0.8 -1.0 nm on specimen axes Rate = 6.40814 A/sec Drift is low enough after shot 2 Measured defocus = -3.63 microns changed by 0.13 to target drift = 0.09 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.07 nm/sec Defocus = -1.019 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.49.58_batch_tomo_13_25_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 122.4 In SetExposureForMean 120, change by a factor of 0.9801 would require too small a change in exposure time Image shift -0.081 0.067 IS units -260.5 156.6 unbinned pixels; need stage -0.046 -0.099 if reset Tilting to -14 4.1 0.7 unbinned pixels; 0.2 1.5 nm along two shift axes; -0.4 1.4 nm on specimen axes Rate = 7.38409 A/sec Drift is low enough after shot 1 Measured defocus = -3.61 microns changed by 0.11 to target drift = 0.07 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -0.899 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.50.57_batch_tomo_13_25_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 122.1 In SetExposureForMean 120, change by a factor of 0.9829 would require too small a change in exposure time Image shift -0.146 0.184 IS units -643.1 221.2 unbinned pixels; need stage -0.056 -0.242 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees 8.9 0.5 unbinned pixels; 0.9 3.0 nm along two shift axes; -0.5 3.1 nm on specimen axes Rate = 15.73512 A/sec 5.5 2.0 unbinned pixels; 0.0 2.0 nm along two shift axes; -0.9 1.9 nm on specimen axes Rate = 10.24921 A/sec Drift is low enough after shot 2 Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.07 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.266 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.52.04_batch_tomo_13_25_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 122.1 In SetExposureForMean 120, change by a factor of 0.9828 would require too small a change in exposure time Image shift -0.048 -0.034 IS units 43.3 163.7 unbinned pixels; need stage -0.058 0.010 if reset Tilting to -4 Correcting tilt backlash -6.4 -3.7 unbinned pixels; 0.5 2.6 nm along two shift axes; 1.5 -2.1 nm on specimen axes Rate = 13.03593 A/sec Drift is low enough after shot 1 Measured defocus = -3.45 microns changed by -0.05 to target drift = 0.08 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.06 nm/sec Defocus = -1.323 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.53.05_batch_tomo_13_25_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 121.5 In SetExposureForMean 120, change by a factor of 0.9877 would require too small a change in exposure time Image shift -0.024 -0.087 IS units 211.9 147.1 unbinned pixels; need stage -0.057 0.070 if reset Tilting to -16 -16.00 degrees 5.8 -7.4 unbinned pixels; 3.1 1.0 nm along two shift axes; 2.4 2.3 nm on specimen axes Rate = 16.52579 A/sec 4.6 1.5 unbinned pixels; 0.0 1.7 nm along two shift axes; -0.7 1.6 nm on specimen axes Rate = 8.52796 A/sec Drift is low enough after shot 2 Measured defocus = -3.64 microns changed by 0.14 to target drift = 0.07 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.08 nm/sec Defocus = -0.779 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.54.11_batch_tomo_13_25_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 121.7 In SetExposureForMean 120, change by a factor of 0.9861 would require too small a change in exposure time Image shift -0.101 0.042 IS units -212.2 235.2 unbinned pixels; need stage -0.074 -0.086 if reset Tilting to -18 -3.6 0.1 unbinned pixels; 0.5 1.2 nm along two shift axes; 0.1 -1.3 nm on specimen axes Rate = 6.28602 A/sec Drift is low enough after shot 1 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.03 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.705 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.55.14_batch_tomo_13_25_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 121.3 In SetExposureForMean 120, change by a factor of 0.9898 would require too small a change in exposure time Image shift -0.112 0.065 IS units -285.7 242.1 unbinned pixels; need stage -0.075 -0.114 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees Trimming A by 130 130, reference by 20 20 Trimming A by 155 155, reference by 20 20 10.7 0.2 unbinned pixels; 1.2 3.6 nm along two shift axes; -0.4 3.7 nm on specimen axes Rate = 18.75849 A/sec Trimming A by 155 155, reference by 20 20 7.0 3.2 unbinned pixels; 0.2 2.7 nm along two shift axes; -1.3 2.4 nm on specimen axes Rate = 13.59144 A/sec Drift is low enough after shot 2 Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.10 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.04 nm/sec Defocus = -1.310 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.56.24_batch_tomo_13_25_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 120.9 In SetExposureForMean 120, change by a factor of 0.9923 would require too small a change in exposure time Image shift -0.056 -0.051 IS units 84.0 201.5 unbinned pixels; need stage -0.072 0.023 if reset Tilting to 0 Correcting tilt backlash Trimming A by 155 155, reference by 145 145 Trimming A by 140 140, reference by 145 145 -6.7 -2.9 unbinned pixels; 0.2 2.6 nm along two shift axes; 1.2 -2.3 nm on specimen axes Rate = 12.78951 A/sec Drift is low enough after shot 1 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.12 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.11 nm/sec Defocus = -1.391 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.57.25_batch_tomo_13_25_009_0.0.tif Saved Z = 9, 0.00 degrees Mean of buffer A = 119.7 In SetExposureForMean 120, change by a factor of 1.0021 would require too small a change in exposure time Image shift -0.032 -0.096 IS units 229.2 178.2 unbinned pixels; need stage -0.068 0.075 if reset Tilting to -20 -20.00 degrees 7.7 31.3 unbinned pixels; 9.5 6.3 nm along two shift axes; -11.2 1.7 nm on specimen axes Rate = 57.05494 A/sec 0.1 9.2 unbinned pixels; 3.0 1.1 nm along two shift axes; -3.2 -0.2 nm on specimen axes Rate = 16.25874 A/sec 0.7 2.1 unbinned pixels; 0.6 0.5 nm along two shift axes; -0.7 0.2 nm on specimen axes Rate = 3.81798 A/sec Drift is low enough after shot 3 Measured defocus = -3.57 microns changed by 0.07 to target drift = 0.02 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.01 nm/sec Defocus = -0.614 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.58.39_batch_tomo_13_25_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 120.4 In SetExposureForMean 120, change by a factor of 0.9968 would require too small a change in exposure time Image shift -0.107 0.032 IS units -192.8 259.8 unbinned pixels; need stage -0.083 -0.082 if reset Tilting to -22 7.2 -1.6 unbinned pixels; 1.4 2.2 nm along two shift axes; 0.3 2.6 nm on specimen axes Rate = 12.89039 A/sec Drift is low enough after shot 1 Measured defocus = -3.55 microns changed by 0.05 to target drift = 0.16 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.10 nm/sec Defocus = -0.548 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_06.59.37_batch_tomo_13_25_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 119.4 In SetExposureForMean 120, change by a factor of 1.0058 would require too small a change in exposure time Image shift -0.095 0.012 IS units -126.6 248.1 unbinned pixels; need stage -0.081 -0.057 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees Trimming A by 110 110, reference by 135 135 Trimming A by 135 135, reference by 135 135 1.9 1.7 unbinned pixels; 0.4 0.8 nm along two shift axes; -0.7 0.6 nm on specimen axes Rate = 4.53006 A/sec Drift is low enough after shot 1 Measured defocus = -3.43 microns changed by -0.07 to target drift = 0.05 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.455 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.00.41_batch_tomo_13_25_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 118.7 In SetExposureForMean 120, change by a factor of 1.0112 would require too small a change in exposure time Image shift -0.071 -0.059 IS units 89.3 248.8 unbinned pixels; need stage -0.088 0.024 if reset Tilting to 4 Correcting tilt backlash Trimming A by 130 130, reference by 125 125 Trimming A by 125 125, reference by 125 125 1.2 0.8 unbinned pixels; 0.1 0.5 nm along two shift axes; -0.3 0.4 nm on specimen axes Rate = 2.47906 A/sec Drift is low enough after shot 1 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.02 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -1.501 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.01.42_batch_tomo_13_25_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 117.4 In SetExposureForMean 120, change by a factor of 1.0223 would require too small a change in exposure time Image shift -0.052 -0.091 IS units 195.1 229.0 unbinned pixels; need stage -0.085 0.062 if reset Tilting to -24 -24.00 degrees -1.9 -5.8 unbinned pixels; 1.7 1.3 nm along two shift axes; 2.1 -0.5 nm on specimen axes Rate = 10.7949 A/sec Drift is low enough after shot 1 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.03 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.483 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.02.44_batch_tomo_13_25_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 118.3 In SetExposureForMean 120, change by a factor of 1.0152 would require too small a change in exposure time Image shift -0.078 -0.061 IS units 87.7 270.0 unbinned pixels; need stage -0.096 0.024 if reset Tilting to -26 6.5 -10.8 unbinned pixels; 4.4 0.8 nm along two shift axes; 3.6 2.6 nm on specimen axes Rate = 22.17166 A/sec 6.8 3.3 unbinned pixels; 0.3 2.6 nm along two shift axes; -1.4 2.3 nm on specimen axes Rate = 13.3059 A/sec Drift is low enough after shot 2 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.12 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.08 nm/sec Defocus = -0.412 um Dewars are NOT busy filling 19 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.03.48_batch_tomo_13_25_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 116.6 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift -0.057 -0.095 IS units 200.6 246.1 unbinned pixels; need stage -0.091 0.070 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees Trimming A by 100 100, reference by 115 120 Trimming A by 110 115, reference by 115 120 -5.5 3.3 unbinned pixels; 1.7 1.4 nm along two shift axes; -1.0 -2.0 nm on specimen axes Rate = 11.28352 A/sec Drift is low enough after shot 1 Measured defocus = -3.41 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.07 nm/sec Defocus = -1.596 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.04.52_batch_tomo_13_25_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 121.8 In SetExposureForMean 120, change by a factor of 0.9850 would require too small a change in exposure time Image shift -0.075 -0.071 IS units 117.4 272.7 unbinned pixels; need stage -0.097 0.033 if reset Tilting to 8 Correcting tilt backlash Trimming A by 115 115, reference by 110 110 Trimming A by 105 105, reference by 110 110 3.6 -0.8 unbinned pixels; 0.7 1.1 nm along two shift axes; 0.2 1.3 nm on specimen axes Rate = 6.48344 A/sec Drift is low enough after shot 1 Measured defocus = -3.43 microns changed by -0.07 to target drift = 0.07 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -1.659 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.05.53_batch_tomo_13_25_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 120.3 In SetExposureForMean 120, change by a factor of 0.9978 would require too small a change in exposure time Image shift -0.055 -0.107 IS units 234.3 251.4 unbinned pixels; need stage -0.094 0.075 if reset Tilting to -28 -28.00 degrees -1.3 -3.2 unbinned pixels; 0.9 0.8 nm along two shift axes; 1.2 -0.4 nm on specimen axes Rate = 6.16827 A/sec Drift is low enough after shot 1 Measured defocus = -3.59 microns changed by 0.09 to target drift = 0.02 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.02 nm/sec Defocus = -0.310 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.06.56_batch_tomo_13_25_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 121.3 In SetExposureForMean 120, change by a factor of 0.9903 would require too small a change in exposure time Image shift -0.048 -0.144 IS units 343.5 267.9 unbinned pixels; need stage -0.103 0.128 if reset Tilting to -30 Trimming A by 75 75, reference by 20 20 2.3 -14.3 unbinned pixels; 5.0 1.0 nm along two shift axes; 4.9 1.2 nm on specimen axes Rate = 25.52996 A/sec 8.5 1.3 unbinned pixels; 0.6 3.0 nm along two shift axes; -0.7 3.0 nm on specimen axes Rate = 15.15693 A/sec 1.3 -0.7 unbinned pixels; 0.4 0.3 nm along two shift axes; 0.2 0.5 nm on specimen axes Rate = 2.57364 A/sec Drift is low enough after shot 3 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.07 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -0.243 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.08.07_batch_tomo_13_25_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 119.4 In SetExposureForMean 120, change by a factor of 1.0060 would require too small a change in exposure time Image shift -0.025 -0.181 IS units 465.9 240.3 unbinned pixels; need stage -0.097 0.181 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees Trimming A by 90 90, reference by 100 100 Trimming A by 95 95, reference by 100 100 -23.3 7.5 unbinned pixels; 5.2 6.8 nm along two shift axes; -1.9 -8.4 nm on specimen axes Rate = 42.86773 A/sec Trimming A by 95 95, reference by 100 100 -19.7 6.6 unbinned pixels; 4.5 5.7 nm along two shift axes; -1.7 -7.1 nm on specimen axes Rate = 36.46275 A/sec Trimming A by 95 100, reference by 100 100 -6.5 0.9 unbinned pixels; 1.0 2.1 nm along two shift axes; -0.1 -2.3 nm on specimen axes Rate = 11.51058 A/sec Drift is low enough after shot 3 Measured defocus = -3.45 microns changed by -0.05 to target drift = 0.09 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.09 nm/sec Defocus = -1.726 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.09.28_batch_tomo_13_25_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 118.3 In SetExposureForMean 120, change by a factor of 1.0143 would require too small a change in exposure time Image shift -0.080 -0.091 IS units 166.9 305.1 unbinned pixels; need stage -0.110 0.050 if reset Tilting to 12 Correcting tilt backlash Trimming A by 115 115, reference by 90 90 Trimming A by 90 90, reference by 90 90 15.0 -3.1 unbinned pixels; 2.8 4.6 nm along two shift axes; 0.6 5.3 nm on specimen axes Rate = 26.77566 A/sec Trimming A by 90 90, reference by 90 90 15.1 4.5 unbinned pixels; 0.3 5.5 nm along two shift axes; -2.0 5.2 nm on specimen axes Rate = 27.70574 A/sec Trimming A by 90 90, reference by 90 90 3.9 1.6 unbinned pixels; 0.1 1.5 nm along two shift axes; -0.7 1.3 nm on specimen axes Rate = 7.37313 A/sec Drift is low enough after shot 3 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.09 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.06 nm/sec Defocus = -1.788 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.10.42_batch_tomo_13_25_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 116.1 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift -0.036 -0.187 IS units 470.8 274.8 unbinned pixels; need stage -0.109 0.161 if reset Tilting to -32 -32.00 degrees 3.0 0.9 unbinned pixels; 0.1 1.1 nm along two shift axes; -0.4 1.0 nm on specimen axes Rate = 5.56195 A/sec Drift is low enough after shot 1 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.05 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -0.191 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.11.45_batch_tomo_13_25_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 123.3 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift -0.010 -0.241 IS units 642.2 257.5 unbinned pixels; need stage -0.108 0.258 if reset Tilting to -34 9.5 -2.7 unbinned pixels; 2.0 2.8 nm along two shift axes; 0.7 3.4 nm on specimen axes Rate = 17.24686 A/sec 2.8 -5.8 unbinned pixels; 2.3 0.2 nm along two shift axes; 2.0 1.2 nm on specimen axes Rate = 11.34822 A/sec Drift is low enough after shot 2 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.07 nm/sec Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.07 nm/sec Defocus = -0.113 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.12.50_batch_tomo_13_25_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 115.4 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift 0.028 -0.318 IS units 889.3 227.4 unbinned pixels; need stage -0.106 0.370 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees Trimming A by 75 85, reference by 85 85 Trimming A by 70 90, reference by 85 85 -32.6 8.6 unbinned pixels; 6.6 9.8 nm along two shift axes; -2.0 -11.7 nm on specimen axes Rate = 59.02471 A/sec Trimming A by 75 90, reference by 85 85 -15.1 5.9 unbinned pixels; 3.7 4.3 nm along two shift axes; -1.6 -5.5 nm on specimen axes Rate = 28.42154 A/sec Trimming A by 70 95, reference by 85 85 -12.2 1.2 unbinned pixels; 1.8 3.9 nm along two shift axes; -0.1 -4.3 nm on specimen axes Rate = 21.51402 A/sec Trimming A by 70 95, reference by 85 85 -5.8 2.8 unbinned pixels; 1.6 1.6 nm along two shift axes; -0.8 -2.1 nm on specimen axes Rate = 11.31963 A/sec Drift is low enough after shot 4 Measured defocus = -3.42 microns changed by -0.08 to target drift = 0.09 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.05 nm/sec Defocus = -1.901 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.14.13_batch_tomo_13_25_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 119.5 In SetExposureForMean 120, change by a factor of 1.0042 would require too small a change in exposure time Image shift -0.059 -0.167 IS units 393.1 320.1 unbinned pixels; need stage -0.122 0.133 if reset Tilting to 16 Correcting tilt backlash Trimming A by 120 120, reference by 80 80 Trimming A by 80 80, reference by 80 80 25.1 -1.8 unbinned pixels; 3.5 8.1 nm along two shift axes; -0.2 8.9 nm on specimen axes Rate = 44.15466 A/sec Trimming A by 80 80, reference by 80 80 12.6 4.9 unbinned pixels; 0.2 4.7 nm along two shift axes; -2.1 4.3 nm on specimen axes Rate = 23.74396 A/sec Trimming A by 75 80, reference by 80 80 4.4 2.9 unbinned pixels; 0.5 1.8 nm along two shift axes; -1.1 1.4 nm on specimen axes Rate = 9.22669 A/sec Drift is low enough after shot 3 Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.06 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.05 nm/sec Defocus = -1.915 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.15.32_batch_tomo_13_25_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 116.8 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.006 -0.312 IS units 850.1 279.7 unbinned pixels; need stage -0.122 0.303 if reset Tilting to -36 -36.00 degrees -0.3 2.5 unbinned pixels; 0.9 0.2 nm along two shift axes; -0.9 -0.2 nm on specimen axes Rate = 4.44194 A/sec Drift is low enough after shot 1 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -0.066 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.16.35_batch_tomo_13_25_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 123.8 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift 0.059 -0.407 IS units 1160.8 227.7 unbinned pixels; need stage -0.114 0.498 if reset Tilting to -38 0.9 13.2 unbinned pixels; 4.3 1.9 nm along two shift axes; -4.7 -0.1 nm on specimen axes Rate = 23.40374 A/sec 6.7 -2.7 unbinned pixels; 1.7 1.9 nm along two shift axes; 0.7 2.4 nm on specimen axes Rate = 12.66191 A/sec Drift is low enough after shot 2 Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.07 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.050 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.17.39_batch_tomo_13_25_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 115.5 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.080 -0.445 IS units 1284.1 206.2 unbinned pixels; need stage -0.110 0.568 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees Trimming A by 60 60, reference by 75 75 Trimming A by 70 70, reference by 75 75 -31.3 8.7 unbinned pixels; 6.5 9.3 nm along two shift axes; -2.1 -11.2 nm on specimen axes Rate = 56.91414 A/sec Trimming A by 70 75, reference by 75 75 -11.4 3.1 unbinned pixels; 2.4 3.4 nm along two shift axes; -0.7 -4.1 nm on specimen axes Rate = 20.74702 A/sec Trimming A by 70 70, reference by 75 75 -7.1 4.6 unbinned pixels; 2.4 1.8 nm along two shift axes; -1.4 -2.6 nm on specimen axes Rate = 14.79173 A/sec Trimming A by 70 75, reference by 75 75 -3.8 1.2 unbinned pixels; 0.8 1.1 nm along two shift axes; -0.3 -1.4 nm on specimen axes Rate = 6.91041 A/sec Drift is low enough after shot 4 Measured defocus = -3.38 microns changed by -0.12 to target drift = 0.09 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.06 nm/sec Defocus = -2.057 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.19.03_batch_tomo_13_25_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 119.1 In SetExposureForMean 120, change by a factor of 1.0080 would require too small a change in exposure time Image shift -0.026 -0.253 IS units 660.5 312.1 unbinned pixels; need stage -0.128 0.235 if reset Tilting to 20 Correcting tilt backlash Trimming A by 105 105, reference by 70 70 Trimming A by 70 75, reference by 70 70 34.8 0.9 unbinned pixels; 3.8 11.6 nm along two shift axes; -1.4 12.2 nm on specimen axes Rate = 61.08871 A/sec Trimming A by 65 70, reference by 70 70 4.9 5.0 unbinned pixels; 1.1 2.2 nm along two shift axes; -1.9 1.6 nm on specimen axes Rate = 12.38311 A/sec Drift is low enough after shot 2 Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.06 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.03 nm/sec Defocus = -2.060 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.20.16_batch_tomo_13_25_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 115.5 In SetExposureForMean 120, exposure time changed to 2.7600 Image shift 0.030 -0.386 IS units 1074.3 285.5 unbinned pixels; need stage -0.131 0.394 if reset Tilting to -40 -40.00 degrees 3.2 10.5 unbinned pixels; 3.1 2.3 nm along two shift axes; -3.8 0.8 nm on specimen axes Rate = 19.34961 A/sec 3.9 -0.7 unbinned pixels; 0.7 1.2 nm along two shift axes; 0.1 1.4 nm on specimen axes Rate = 7.01233 A/sec Drift is low enough after shot 2 Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.05 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.02 nm/sec Defocus = -0.006 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.21.26_batch_tomo_13_25_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 123.1 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift 0.104 -0.506 IS units 1472.9 197.8 unbinned pixels; need stage -0.113 0.671 if reset Tilting to -42 5.3 14.1 unbinned pixels; 4.1 3.4 nm along two shift axes; -5.1 1.4 nm on specimen axes Rate = 26.67063 A/sec 7.0 -0.2 unbinned pixels; 0.9 2.3 nm along two shift axes; -0.2 2.5 nm on specimen axes Rate = 12.33466 A/sec Drift is low enough after shot 2 Measured defocus = -3.55 microns changed by 0.05 to target drift = 0.09 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.06 nm/sec Defocus = 0.012 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.22.31_batch_tomo_13_25_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 114.2 In SetExposureForMean 120, exposure time changed to 2.7600 Image shift 0.124 -0.537 IS units 1576.8 174.3 unbinned pixels; need stage -0.109 0.743 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees Trimming A by 35 35, reference by 65 65 Trimming A by 60 65, reference by 65 65 -12.7 3.5 unbinned pixels; 2.6 3.8 nm along two shift axes; -0.8 -4.5 nm on specimen axes Rate = 22.99107 A/sec Trimming A by 60 65, reference by 65 65 -6.1 4.4 unbinned pixels; 2.2 1.5 nm along two shift axes; -1.4 -2.3 nm on specimen axes Rate = 13.17132 A/sec Drift is low enough after shot 2 Measured defocus = -3.37 microns changed by -0.13 to target drift = 0.16 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.12 nm/sec Defocus = -2.180 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.23.43_batch_tomo_13_25_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 117.1 In SetExposureForMean 120, exposure time changed to 2.8800 Image shift -0.001 -0.313 IS units 846.8 301.3 unbinned pixels; need stage -0.130 0.312 if reset Tilting to 24 Correcting tilt backlash Trimming A by 90 95, reference by 60 60 Trimming A by 60 60, reference by 60 60 -0.8 -1.1 unbinned pixels; 0.3 0.4 nm along two shift axes; 0.4 -0.2 nm on specimen axes Rate = 2.37467 A/sec Drift is low enough after shot 1 Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.08 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.07 nm/sec Defocus = -2.172 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.24.45_batch_tomo_13_25_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 118.0 In SetExposureForMean 120, change by a factor of 1.0183 would require too small a change in exposure time Image shift 0.041 -0.426 IS units 1195.0 294.3 unbinned pixels; need stage -0.138 0.452 if reset Tilting to -44 -44.00 degrees 34.9 5.6 unbinned pixels; 2.2 12.2 nm along two shift axes; -3.0 12.1 nm on specimen axes Rate = 62.10166 A/sec 4.6 3.6 unbinned pixels; 0.6 2.0 nm along two shift axes; -1.4 1.5 nm on specimen axes Rate = 10.29522 A/sec Drift is low enough after shot 2 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.04 nm/sec Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.06 nm/sec Defocus = 0.111 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.25.55_batch_tomo_13_25_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 120.6 In SetExposureForMean 120, change by a factor of 0.9963 would require too small a change in exposure time Image shift 0.167 -0.630 IS units 1872.7 145.4 unbinned pixels; need stage -0.108 0.914 if reset Tilting to -46 7.4 -1.2 unbinned pixels; 1.3 2.3 nm along two shift axes; 0.2 2.6 nm on specimen axes Rate = 13.22417 A/sec Drift is low enough after shot 1 Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.05 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.03 nm/sec Defocus = 0.101 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.26.55_batch_tomo_13_25_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 116.0 In SetExposureForMean 120, exposure time changed to 3.0000 Image shift 0.187 -0.667 IS units 1990.2 125.1 unbinned pixels; need stage -0.104 1.007 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees Trimming A by 40 40, reference by 55 60 Trimming A by 50 55, reference by 55 60 25.1 -70.1 unbinned pixels; 26.2 0.1 nm along two shift axes; 23.8 10.9 nm on specimen axes Rate = 130.9702 A/sec Trimming A by 45 50, reference by 55 60 -5.1 6.3 unbinned pixels; 2.7 0.9 nm along two shift axes; -2.0 -2.0 nm on specimen axes Rate = 14.2532 A/sec Trimming A by 45 50, reference by 55 60 -0.9 1.6 unbinned pixels; 0.7 0.1 nm along two shift axes; -0.5 -0.4 nm on specimen axes Rate = 3.30977 A/sec Drift is low enough after shot 3 Measured defocus = -3.41 microns changed by -0.09 to target drift = 0.09 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.09 nm/sec Defocus = -2.291 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.28.14_batch_tomo_13_25_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 117.7 In SetExposureForMean 120, exposure time changed to 3.1200 Image shift 0.024 -0.382 IS units 1058.4 297.8 unbinned pixels; need stage -0.135 0.406 if reset Tilting to 28 Correcting tilt backlash Trimming A by 90 90, reference by 50 50 Trimming A by 40 40, reference by 50 50 4.7 -2.9 unbinned pixels; 1.5 1.2 nm along two shift axes; 0.9 1.7 nm on specimen axes Rate = 9.65086 A/sec Drift is low enough after shot 1 Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.14 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.07 nm/sec Defocus = -2.270 um Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.29.15_batch_tomo_13_25_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 117.7 In SetExposureForMean 120, exposure time changed to 3.2400 Image shift 0.094 -0.504 IS units 1457.9 222.4 unbinned pixels; need stage -0.121 0.576 if reset Tilting to -48 -48.00 degrees 15.1 -20.3 unbinned pixels; 8.5 2.6 nm along two shift axes; 6.7 5.9 nm on specimen axes Rate = 44.47254 A/sec 3.9 -2.3 unbinned pixels; 1.2 1.0 nm along two shift axes; 0.7 1.4 nm on specimen axes Rate = 7.89349 A/sec Drift is low enough after shot 2 Measured defocus = -3.59 microns changed by 0.09 to target drift = 0.12 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.07 nm/sec Defocus = 0.194 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.30.26_batch_tomo_13_25_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 125.2 In SetExposureForMean 120, exposure time changed to 3.1200 Image shift 0.210 -0.698 IS units 2096.7 93.4 unbinned pixels; need stage -0.097 1.103 if reset Tilting to -50 16.3 -7.7 unbinned pixels; 4.5 4.5 nm along two shift axes; 2.2 6.0 nm on specimen axes Rate = 31.61019 A/sec 5.7 3.1 unbinned pixels; 0.4 2.2 nm along two shift axes; -1.3 1.9 nm on specimen axes Rate = 11.36934 A/sec Drift is low enough after shot 2 Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.06 nm/sec Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.05 nm/sec Defocus = 0.177 um Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.31.31_batch_tomo_13_25_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 115.2 In SetExposureForMean 120, exposure time changed to 3.2400 Image shift 0.228 -0.744 IS units 2239.1 88.0 unbinned pixels; need stage -0.099 1.227 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees Trimming A by 40 45, reference by 40 45 Trimming A by 40 40, reference by 40 45 -8.4 5.1 unbinned pixels; 2.7 2.2 nm along two shift axes; -1.5 -3.1 nm on specimen axes Rate = 17.17836 A/sec Trimming A by 40 40, reference by 40 45 -6.6 1.6 unbinned pixels; 1.3 2.0 nm along two shift axes; -0.3 -2.4 nm on specimen axes Rate = 11.97582 A/sec Drift is low enough after shot 2 Measured defocus = -3.43 microns changed by -0.07 to target drift = 0.14 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.15 nm/sec Defocus = -2.340 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.32.45_batch_tomo_13_25_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 116.6 In SetExposureForMean 120, exposure time changed to 3.3600 Image shift 0.074 -0.507 IS units 1447.7 280.3 unbinned pixels; need stage -0.141 0.581 if reset Tilting to 32 Correcting tilt backlash Trimming A by 80 85, reference by 40 40 Trimming A by 40 40, reference by 40 40 -10.7 -4.2 unbinned pixels; 0.1 4.0 nm along two shift axes; 1.8 -3.6 nm on specimen axes Rate = 20.17004 A/sec Trimming A by 40 40, reference by 40 40 -7.2 4.0 unbinned pixels; 2.2 1.9 nm along two shift axes; -1.2 -2.7 nm on specimen axes Rate = 14.51021 A/sec Trimming A by 40 40, reference by 40 40 -0.5 1.7 unbinned pixels; 0.6 0.0 nm along two shift axes; -0.6 -0.2 nm on specimen axes Rate = 3.05215 A/sec Drift is low enough after shot 3 Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.06 nm/sec Measured defocus = -3.46 microns changed by -0.04 to target drift = 0.07 nm/sec Defocus = -2.333 um Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.33.59_batch_tomo_13_25_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 115.1 In SetExposureForMean 120, exposure time changed to 3.4800 Image shift 0.130 -0.620 IS units 1808.3 237.0 unbinned pixels; need stage -0.137 0.745 if reset Tilting to -52 -52.00 degrees 21.6 -7.5 unbinned pixels; 5.0 6.2 nm along two shift axes; 2.0 7.8 nm on specimen axes Rate = 40.01341 A/sec 83.4 -52.6 unbinned pixels; 27.2 21.3 nm along two shift axes; 15.9 30.9 nm on specimen axes Rate = 172.79253 A/sec 5.3 -2.2 unbinned pixels; 1.4 1.5 nm along two shift axes; 0.6 1.9 nm on specimen axes Rate = 9.99504 A/sec Drift is low enough after shot 3 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.09 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = 0.245 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.35.22_batch_tomo_13_25_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 122.3 In SetExposureForMean 120, exposure time changed to 3.3600 Image shift 0.247 -0.764 IS units 2314.1 55.5 unbinned pixels; need stage -0.090 1.326 if reset Tilting to -54 9.3 -5.3 unbinned pixels; 2.8 2.4 nm along two shift axes; 1.6 3.4 nm on specimen axes Rate = 18.71543 A/sec 9.8 3.1 unbinned pixels; 0.1 3.6 nm along two shift axes; -1.4 3.4 nm on specimen axes Rate = 18.14077 A/sec 1.2 1.0 unbinned pixels; 0.2 0.5 nm along two shift axes; -0.4 0.4 nm on specimen axes Rate = 2.78344 A/sec Drift is low enough after shot 3 Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.08 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.10 nm/sec Defocus = 0.191 um Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.36.34_batch_tomo_13_25_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 112.5 In SetExposureForMean 120, exposure time changed to 3.6000 Image shift 0.264 -0.822 IS units 2486.1 63.9 unbinned pixels; need stage -0.098 1.492 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees Trimming A by 35 35, reference by 40 40 Trimming A by 35 35, reference by 40 40 -13.8 3.6 unbinned pixels; 2.8 4.1 nm along two shift axes; -0.8 -4.9 nm on specimen axes Rate = 24.99593 A/sec Trimming A by 35 35, reference by 40 40 -10.2 2.4 unbinned pixels; 2.0 3.1 nm along two shift axes; -0.5 -3.6 nm on specimen axes Rate = 18.28556 A/sec Trimming A by 35 35, reference by 40 40 -9.3 2.1 unbinned pixels; 1.8 2.8 nm along two shift axes; -0.5 -3.3 nm on specimen axes Rate = 16.74852 A/sec Trimming A by 35 35, reference by 40 40 -6.6 2.0 unbinned pixels; 1.4 2.0 nm along two shift axes; -0.5 -2.4 nm on specimen axes Rate = 12.14591 A/sec Drift is low enough after shot 4 Measured defocus = -3.44 microns changed by -0.06 to target drift = 0.14 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.15 nm/sec Defocus = -2.392 um Dewars are NOT busy filling 30 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.38.01_batch_tomo_13_25_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 116.2 In SetExposureForMean 120, exposure time changed to 3.7200 Image shift 0.118 -0.608 IS units 1763.9 257.5 unbinned pixels; need stage -0.143 0.742 if reset Tilting to 36 Correcting tilt backlash Trimming A by 85 85, reference by 35 35 Trimming A by 35 35, reference by 35 35 -12.6 -7.1 unbinned pixels; 0.9 5.0 nm along two shift axes; 2.9 -4.2 nm on specimen axes Rate = 25.45939 A/sec Trimming A by 35 35, reference by 35 35 -6.5 4.4 unbinned pixels; 2.2 1.6 nm along two shift axes; -1.3 -2.4 nm on specimen axes Rate = 13.72508 A/sec Drift is low enough after shot 2 Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.09 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.11 nm/sec Defocus = -2.346 um Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars ARE busy filling Dewars are filling Dewars are NOT busy filling 31 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.48.14_batch_tomo_13_25_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 112.3 In SetExposureForMean 120, exposure time changed to 3.9600 Image shift 0.165 -0.741 IS units 2170.6 255.9 unbinned pixels; need stage -0.155 0.938 if reset Tilting to -56 -56.00 degrees 8.5 -1.4 unbinned pixels; 1.4 2.6 nm along two shift axes; 0.2 3.0 nm on specimen axes Rate = 15.01679 A/sec 8.9 0.7 unbinned pixels; 0.8 3.0 nm along two shift axes; -0.5 3.1 nm on specimen axes Rate = 15.62429 A/sec 5.3 -0.0 unbinned pixels; 0.6 1.7 nm along two shift axes; -0.2 1.9 nm on specimen axes Rate = 9.27905 A/sec Drift is low enough after shot 3 Measured defocus = -3.61 microns changed by 0.11 to target drift = 0.13 nm/sec Measured defocus = -3.59 microns changed by 0.09 to target drift = 0.12 nm/sec Defocus = 0.386 um Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.49.32_batch_tomo_13_25_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 125.0 In SetExposureForMean 120, exposure time changed to 3.8400 Image shift 0.252 -0.853 IS units 2560.3 126.5 unbinned pixels; need stage -0.122 1.612 if reset Tilting to -58 10.9 -7.9 unbinned pixels; 3.9 2.7 nm along two shift axes; 2.4 4.1 nm on specimen axes Rate = 23.63103 A/sec 3.5 1.3 unbinned pixels; 0.0 1.3 nm along two shift axes; -0.6 1.2 nm on specimen axes Rate = 6.66862 A/sec Drift is low enough after shot 2 Measured defocus = -3.38 microns changed by -0.12 to target drift = 0.08 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.08 nm/sec Defocus = 0.249 um Dewars are NOT busy filling 32 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.50.39_batch_tomo_13_25_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 113.2 In SetExposureForMean 120, exposure time changed to 4.0800 Image shift 0.302 -0.959 IS units 2895.2 91.3 unbinned pixels; need stage -0.121 1.926 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees Trimming A by 40 40, reference by 30 35 Trimming A by 30 30, reference by 30 35 -17.6 1.1 unbinned pixels; 2.4 5.7 nm along two shift axes; 0.1 -6.2 nm on specimen axes Rate = 31.00325 A/sec Trimming A by 30 30, reference by 30 35 -8.8 2.1 unbinned pixels; 1.7 2.7 nm along two shift axes; -0.5 -3.2 nm on specimen axes Rate = 15.87936 A/sec Trimming A by 30 30, reference by 30 35 -8.8 2.2 unbinned pixels; 1.8 2.6 nm along two shift axes; -0.5 -3.1 nm on specimen axes Rate = 15.83494 A/sec Trimming A by 30 30, reference by 30 35 -5.1 1.4 unbinned pixels; 1.1 1.5 nm along two shift axes; -0.3 -1.8 nm on specimen axes Rate = 9.32117 A/sec Drift is low enough after shot 4 Measured defocus = -3.38 microns changed by -0.12 to target drift = 0.16 nm/sec Measured defocus = -3.50 microns changed by -0.00 to target drift = 0.15 nm/sec Defocus = -2.470 um Dewars are NOT busy filling 34 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.52.06_batch_tomo_13_25_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 115.1 In SetExposureForMean 120, exposure time changed to 4.3200 Image shift 0.150 -0.761 IS units 2210.8 316.1 unbinned pixels; need stage -0.177 0.979 if reset Tilting to 40 Correcting tilt backlash Trimming A by 70 70, reference by 30 30 Trimming A by 25 30, reference by 30 30 -12.5 -6.8 unbinned pixels; 0.8 5.0 nm along two shift axes; 2.8 -4.2 nm on specimen axes Rate = 25.16678 A/sec Trimming A by 25 30, reference by 30 30 -2.7 3.5 unbinned pixels; 1.5 0.5 nm along two shift axes; -1.2 -1.0 nm on specimen axes Rate = 7.77886 A/sec Drift is low enough after shot 2 Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.11 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.06 nm/sec Defocus = -2.496 um Dewars are NOT busy filling 36 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.53.16_batch_tomo_13_25_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 112.7 In SetExposureForMean 120, exposure time changed to 4.6800 Image shift 0.226 -0.873 IS units 2586.0 216.4 unbinned pixels; need stage -0.154 1.184 if reset Tilting to -60 -60.00 degrees Trimming A by 20 20, reference by 180 180 Trimming A by 20 20, reference by 180 180 99.7 -998.1 unbinned pixels; 343.1 86.5 nm along two shift axes; 346.9 65.6 nm on specimen axes Rate = 1769.34328 A/sec Trimming A by 20 20, reference by 180 180 -108.0 1104.4 unbinned pixels; 379.4 96.5 nm along two shift axes; -384.0 -71.8 nm on specimen axes Rate = 1957.44869 A/sec Trimming A by 20 20, reference by 180 180 7.5 31.0 unbinned pixels; 9.4 6.2 nm along two shift axes; -11.1 1.7 nm on specimen axes Rate = 56.34795 A/sec Trimming A by 20 20, reference by 180 180 0.8 -1.8 unbinned pixels; 0.7 0.0 nm along two shift axes; 0.6 0.3 nm on specimen axes Rate = 3.35164 A/sec Drift is low enough after shot 4 Measured defocus = -3.81 microns changed by 0.31 to target drift = 0.13 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = 0.570 um Dewars are NOT busy filling 39 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.54.41_batch_tomo_13_25_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 143.2 In SetExposureForMean 120, exposure time changed to 3.9600 Image shift 0.280 -0.949 IS units 2848.3 141.0 unbinned pixels; need stage -0.136 2.005 if reset Tilting to -62 Trimming A by 20 20, reference by 185 185 Trimming A by 20 20, reference by 185 185 12.4 22.5 unbinned pixels; 6.0 6.8 nm along two shift axes; -8.3 3.7 nm on specimen axes Rate = 45.42967 A/sec Trimming A by 20 20, reference by 185 185 6.5 2.1 unbinned pixels; 0.0 2.4 nm along two shift axes; -1.0 2.2 nm on specimen axes Rate = 12.01689 A/sec Drift is low enough after shot 2 Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.09 nm/sec Measured defocus = -3.50 microns changed by 0.00 to target drift = 0.09 nm/sec Defocus = 0.583 um Dewars are NOT busy filling 33 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.55.49_batch_tomo_13_25_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 111.8 In SetExposureForMean 120, exposure time changed to 4.2000 Image shift 0.294 -0.992 IS units 2976.3 142.5 unbinned pixels; need stage -0.141 2.232 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees Trimming A by 25 25, reference by 25 30 Trimming A by 25 25, reference by 25 30 -15.6 2.6 unbinned pixels; 2.7 4.9 nm along two shift axes; -0.4 -5.6 nm on specimen axes Rate = 27.79689 A/sec Trimming A by 25 25, reference by 25 30 -13.0 3.2 unbinned pixels; 2.6 3.9 nm along two shift axes; -0.7 -4.7 nm on specimen axes Rate = 23.48628 A/sec Trimming A by 25 25, reference by 25 30 -8.1 2.2 unbinned pixels; 1.7 2.4 nm along two shift axes; -0.5 -2.9 nm on specimen axes Rate = 14.67006 A/sec Trimming A by 25 25, reference by 25 30 -7.5 2.0 unbinned pixels; 1.5 2.2 nm along two shift axes; -0.5 -2.7 nm on specimen axes Rate = 13.64775 A/sec Drift is low enough after shot 4 Measured defocus = -3.38 microns changed by -0.12 to target drift = 0.19 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.16 nm/sec Defocus = -2.604 um Dewars are NOT busy filling 35 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.57.21_batch_tomo_13_25_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 101.1 In SetExposureForMean 120, exposure time changed to 5.0400 Image shift 0.215 -0.878 IS units 2591.5 250.0 unbinned pixels; need stage -0.166 1.222 if reset Tilting to 44 Correcting tilt backlash Trimming A by 60 65, reference by 20 20 -10.4 -6.3 unbinned pixels; 0.9 4.2 nm along two shift axes; 2.5 -3.5 nm on specimen axes Rate = 21.47278 A/sec -7.1 4.2 unbinned pixels; 2.2 1.9 nm along two shift axes; -1.2 -2.6 nm on specimen axes Rate = 14.46946 A/sec -3.0 1.1 unbinned pixels; 0.7 0.9 nm along two shift axes; -0.3 -1.1 nm on specimen axes Rate = 5.52544 A/sec Drift is low enough after shot 3 Measured defocus = -3.52 microns changed by 0.02 to target drift = 0.11 nm/sec Measured defocus = -3.51 microns changed by 0.01 to target drift = 0.14 nm/sec Defocus = -2.571 um Dewars are NOT busy filling 42 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.58.36_batch_tomo_13_25_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 109.5 In SetExposureForMean 120, exposure time changed to 5.6400 Image shift 0.271 -0.987 IS units 2941.8 202.2 unbinned pixels; need stage -0.160 1.437 if reset Tilting to -64 -64.00 degrees 5.1 -6.8 unbinned pixels; 2.8 0.9 nm along two shift axes; 2.2 2.0 nm on specimen axes Rate = 14.87909 A/sec 5.6 2.3 unbinned pixels; 0.1 2.1 nm along two shift axes; -1.0 1.9 nm on specimen axes Rate = 10.62734 A/sec Drift is low enough after shot 2 Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.15 nm/sec Measured defocus = -3.49 microns changed by -0.01 to target drift = 0.04 nm/sec Defocus = 0.549 um Dewars are NOT busy filling 47 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_07.59.50_batch_tomo_13_25_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 129.4 In SetExposureForMean 120, exposure time changed to 5.1600 Image shift 0.330 -1.049 IS units 3167.8 101.3 unbinned pixels; need stage -0.132 2.548 if reset Tilting to -66 5.7 -7.6 unbinned pixels; 3.2 1.0 nm along two shift axes; 2.5 2.2 nm on specimen axes Rate = 16.68093 A/sec 2.7 2.1 unbinned pixels; 0.4 1.2 nm along two shift axes; -0.8 0.9 nm on specimen axes Rate = 6.09401 A/sec Drift is low enough after shot 2 Measured defocus = -3.34 microns changed by -0.16 to target drift = 0.09 nm/sec Measured defocus = -3.54 microns changed by 0.04 to target drift = 0.07 nm/sec Defocus = 0.436 um Dewars are NOT busy filling 43 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.00.59_batch_tomo_13_25_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 106.2 In SetExposureForMean 120, exposure time changed to 5.7600 Image shift 0.381 -1.125 IS units 3423.1 34.4 unbinned pixels; need stage -0.117 2.973 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees Trimming A by 35 35, reference by 20 20 -22.3 0.9 unbinned pixels; 2.9 7.3 nm along two shift axes; 0.4 -7.8 nm on specimen axes Rate = 39.10302 A/sec -8.7 2.2 unbinned pixels; 1.7 2.6 nm along two shift axes; -0.5 -3.1 nm on specimen axes Rate = 15.7058 A/sec -7.4 1.4 unbinned pixels; 1.4 2.3 nm along two shift axes; -0.3 -2.7 nm on specimen axes Rate = 13.29442 A/sec Drift is low enough after shot 3 Measured defocus = -3.38 microns changed by -0.12 to target drift = 0.18 nm/sec Measured defocus = -3.48 microns changed by -0.02 to target drift = 0.08 nm/sec Defocus = -2.715 um Dewars are NOT busy filling 48 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.02.21_batch_tomo_13_25_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 112.2 In SetExposureForMean 120, exposure time changed to 6.2400 Image shift 0.272 -1.028 IS units 3053.0 237.4 unbinned pixels; need stage -0.176 1.543 if reset Tilting to 48 Correcting tilt backlash Trimming A by 65 70, reference by 20 20 Trimming A by 20 25, reference by 20 20 -10.6 -8.3 unbinned pixels; 1.5 4.5 nm along two shift axes; 3.2 -3.4 nm on specimen axes Rate = 23.71282 A/sec Trimming A by 20 25, reference by 20 20 -6.4 4.5 unbinned pixels; 2.2 1.6 nm along two shift axes; -1.4 -2.4 nm on specimen axes Rate = 13.62367 A/sec Drift is low enough after shot 2 Measured defocus = -3.56 microns changed by 0.06 to target drift = 0.06 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.05 nm/sec Defocus = -2.680 um Dewars are NOT busy filling 52 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.03.32_batch_tomo_13_25_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 106.8 In SetExposureForMean 120, exposure time changed to 7.2000 Image shift 0.347 -1.148 IS units 3451.7 147.5 unbinned pixels; need stage -0.157 1.817 if reset Tilting to -68 -68.00 degrees 1.1 -1.6 unbinned pixels; 0.7 0.2 nm along two shift axes; 0.5 0.5 nm on specimen axes Rate = 3.46902 A/sec Drift is low enough after shot 1 Measured defocus = -3.67 microns changed by 0.17 to target drift = 0.15 nm/sec Measured defocus = -3.47 microns changed by -0.03 to target drift = 0.16 nm/sec Defocus = 0.576 um Dewars are NOT busy filling 60 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.04.47_batch_tomo_13_25_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 132.0 In SetExposureForMean 120, exposure time changed to 6.4800 Image shift 0.388 -1.148 IS units 3491.3 35.8 unbinned pixels; need stage -0.120 3.292 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 0.89 microns changed by -4.39 to target drift = 0.49 nm/sec Focus area changed from stored state, axis position -2.50 angle 0, subarea offset 0 0 Opened new file D:\Users\Ben\210112_Titan2_Jans_cells\SEM\batch_tomo_14.st Running script 1 at item 27 (-540.49, -257.10) 08:05:50 Refine ZLP: additional offset = -0.1 eV; total offset = -15.0 eV Item 27: Stage: -540.49 -257.10 -223 Label: 27 Note: Sec 26 - anchor_maps.map Target defocus is: -3.50 um Target defocus is: -3.75 um Target defocus = -3.75 Trimming A by 20 65, reference by 20 60 Angle = -4.40, increment = 0.60, shift = -27.8 -7.7 pixels, -0.065 um Y Trimming A by 20 65, reference by 20 60 Angle = -3.20, increment = 1.80, shift = -104.5 -22.6 pixels, -0.243 um Y Trimming A by 40 40, reference by 20 60 Angle = 0.40, increment = 5.41, shift = -344.3 -57.7 pixels, -0.796 um Y Trimming A by 20 30, reference by 20 60 Angle = 3.00, increment = 8.00, shift = -500.0 -84.4 pixels, -1.156 um Y Rough eucentricity: changing Z by 8.29 to -215.09, finished. Angle = 24.00, shift = 0.0, 0.0 pixels, 0.000, 0.000 um, IS = 0.000, 0.000 Trimming A by 20 65, reference by 20 65 Angle = 18.00, shift = -73.3, 1.3 pixels, 0.022, -0.166 um, IS = -0.022, 0.166 Trimming A by 22 65, reference by 20 65 Angle = 11.00, shift = -74.2, -6.0 pixels, 0.005, -0.170 um, IS = -0.027, 0.336 Trimming A by 22 65, reference by 22 65 Angle = 4.00, shift = -60.4, -4.4 pixels, 0.006, -0.138 um, IS = -0.032, 0.474 Trimming A by 23 55 40 20, reference by 23 55 40 20 Angle = -4.00, shift = -33.2, -21.1 pixels, -0.039, -0.081 um, IS = 0.007, 0.555 Trimming A by 22 65, reference by 22 70 Angle = -11.00, shift = -39.5, -16.4 pixels, -0.027, -0.094 um, IS = 0.034, 0.649 Trimming A by 22 65, reference by 21 65 Angle = -18.00, shift = -67.6, -2.9 pixels, 0.011, -0.154 um, IS = 0.024, 0.803 Trimming A by 20 65, reference by 20 65 Angle = -24.00, shift = -3.3, 3.7 pixels, 0.009, -0.007 um, IS = 0.014, 0.809 General solution delZ 0.98, delY 0.70, yZero 0.52 solution with assumed YZero 0.51: delZ 0.98, delY 0.60 Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 0.98 microns and lateral displacement of -0.70 microns Trimming A by 24 45 30 20, reference by 21 20 25 20 Disparity in stage position after aligning to center of map frame: 0.07 -1.42 Trimming A by 21 20 25 20, reference by 21 20 25 20 Disparity in stage position after aligning to target position: 0.48 -1.89 (2nd move error 0.04 0.06) Disparity in stage position after aligning to map item itself: 0.09 -1.35 (align shift -0.02 -0.02) Finished aligning to item in 3 rounds Setting Eucentric Focus! Tilting to -10 -10.00 degrees Stage -540.41 -258.48 -216.06 -0.1 -1.6 unbinned pixels; 0.5 0.2 nm along two shift axes; 0.6 0.0 nm on specimen axes Rate = 2.78021 A/sec Drift is low enough after shot 1 Measured defocus = 1.88 microns changed by -5.63 to target drift = 0.03 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.04 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.06 nm/sec Defocus = -1.769 um Dewars are NOT busy filling 10 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.10.58_batch_tomo_14_27_000_-10.0.tif Saved Z = 0, -10.00 degrees Mean of buffer A = 58.9 In SetExposureForMean 120, exposure time changed to 2.4000 Image shift 0.031 -0.028 IS units 105.9 -57.4 unbinned pixels; need stage 0.017 0.040 if reset Tilting to -8 Correcting tilt backlash 8.4 3.6 unbinned pixels; 0.2 3.2 nm along two shift axes; -1.5 2.8 nm on specimen axes Rate = 16.09513 A/sec 3.8 0.3 unbinned pixels; 0.4 1.3 nm along two shift axes; -0.2 1.3 nm on specimen axes Rate = 6.72392 A/sec Drift is low enough after shot 2 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.06 nm/sec Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -1.798 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.12.03_batch_tomo_14_27_001_-8.0.tif Saved Z = 1, -8.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, change by a factor of 1.0213 would require too small a change in exposure time Image shift -0.068 0.090 IS units -311.0 100.9 unbinned pixels; need stage -0.025 -0.114 if reset Tilting to -12 -12.00 degrees -5.8 9.3 unbinned pixels; 3.8 0.8 nm along two shift axes; -3.1 -2.3 nm on specimen axes Rate = 19.23597 A/sec -3.3 2.3 unbinned pixels; 1.1 0.8 nm along two shift axes; -0.7 -1.2 nm on specimen axes Rate = 7.06563 A/sec Drift is low enough after shot 2 Measured defocus = -3.85 microns changed by 0.10 to target drift = 0.09 nm/sec Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.07 nm/sec Defocus = -1.641 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.13.08_batch_tomo_14_27_002_-12.0.tif Saved Z = 2, -12.00 degrees Mean of buffer A = 118.4 In SetExposureForMean 120, change by a factor of 1.0133 would require too small a change in exposure time Image shift -0.109 0.161 IS units -543.7 143.2 unbinned pixels; need stage -0.033 -0.201 if reset Tilting to -14 7.8 -0.7 unbinned pixels; 1.2 2.5 nm along two shift axes; 0.0 2.8 nm on specimen axes Rate = 13.75892 A/sec Drift is low enough after shot 1 Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.16 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.11 nm/sec Defocus = -1.585 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.14.06_batch_tomo_14_27_003_-14.0.tif Saved Z = 3, -14.00 degrees Mean of buffer A = 117.9 In SetExposureForMean 120, change by a factor of 1.0175 would require too small a change in exposure time Image shift -0.119 0.184 IS units -617.3 149.5 unbinned pixels; need stage -0.032 -0.230 if reset Tilting to -6 Correcting tilt backlash -6.00 degrees -1.6 1.3 unbinned pixels; 0.6 0.4 nm along two shift axes; -0.4 -0.6 nm on specimen axes Rate = 3.65334 A/sec Drift is low enough after shot 1 Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.02 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.00 nm/sec Defocus = -1.861 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.15.07_batch_tomo_14_27_004_-6.0.tif Saved Z = 4, -6.00 degrees Mean of buffer A = 117.8 In SetExposureForMean 120, change by a factor of 1.0189 would require too small a change in exposure time Image shift -0.096 0.130 IS units -446.8 136.7 unbinned pixels; need stage -0.033 -0.163 if reset Tilting to -4 Correcting tilt backlash 4.2 -1.3 unbinned pixels; 0.9 1.2 nm along two shift axes; 0.3 1.5 nm on specimen axes Rate = 7.68949 A/sec Drift is low enough after shot 1 Measured defocus = -3.67 microns changed by -0.08 to target drift = 0.07 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.03 nm/sec Defocus = -1.926 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.16.08_batch_tomo_14_27_005_-4.0.tif Saved Z = 5, -4.00 degrees Mean of buffer A = 117.6 In SetExposureForMean 120, change by a factor of 1.0207 would require too small a change in exposure time Image shift -0.093 0.128 IS units -437.5 130.6 unbinned pixels; need stage -0.031 -0.159 if reset Tilting to -16 -16.00 degrees -1.8 0.3 unbinned pixels; 0.3 0.6 nm along two shift axes; -0.0 -0.6 nm on specimen axes Rate = 3.24429 A/sec Drift is low enough after shot 1 Measured defocus = -3.81 microns changed by 0.06 to target drift = 0.05 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -1.518 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.17.08_batch_tomo_14_27_006_-16.0.tif Saved Z = 6, -16.00 degrees Mean of buffer A = 117.8 In SetExposureForMean 120, change by a factor of 1.0184 would require too small a change in exposure time Image shift -0.120 0.158 IS units -547.2 174.9 unbinned pixels; need stage -0.043 -0.207 if reset Tilting to -18 9.0 0.1 unbinned pixels; 1.0 3.0 nm along two shift axes; -0.3 3.2 nm on specimen axes Rate = 15.8414 A/sec 6.7 0.0 unbinned pixels; 0.8 2.2 nm along two shift axes; -0.2 2.4 nm on specimen axes Rate = 11.83887 A/sec Drift is low enough after shot 2 Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.10 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.10 nm/sec Defocus = -1.460 um Dewars are NOT busy filling 20 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.18.11_batch_tomo_14_27_007_-18.0.tif Saved Z = 7, -18.00 degrees Mean of buffer A = 116.4 In SetExposureForMean 120, exposure time changed to 2.5200 Image shift -0.129 0.183 IS units -624.1 177.6 unbinned pixels; need stage -0.042 -0.238 if reset Tilting to -2 Correcting tilt backlash -2.00 degrees 0.1 1.9 unbinned pixels; 0.6 0.3 nm along two shift axes; -0.7 -0.0 nm on specimen axes Rate = 3.40838 A/sec Drift is low enough after shot 1 Measured defocus = -3.62 microns changed by -0.13 to target drift = 0.03 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -2.045 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.19.13_batch_tomo_14_27_008_-2.0.tif Saved Z = 8, -2.00 degrees Mean of buffer A = 122.7 In SetExposureForMean 120, change by a factor of 0.9777 would require too small a change in exposure time Image shift -0.121 0.165 IS units -566.2 171.5 unbinned pixels; need stage -0.042 -0.206 if reset Tilting to 0 Correcting tilt backlash 6.9 -1.0 unbinned pixels; 1.1 2.2 nm along two shift axes; 0.1 2.5 nm on specimen axes Rate = 12.25668 A/sec Drift is low enough after shot 1 Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.08 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.05 nm/sec Defocus = -2.115 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.20.14_batch_tomo_14_27_009_-0.0.tif Saved Z = 9, -0.00 degrees Mean of buffer A = 122.0 In SetExposureForMean 120, change by a factor of 0.9840 would require too small a change in exposure time Image shift -0.116 0.156 IS units -537.6 167.0 unbinned pixels; need stage -0.041 -0.195 if reset Tilting to -20 -20.00 degrees -3.5 -0.1 unbinned pixels; 0.4 1.2 nm along two shift axes; 0.1 -1.2 nm on specimen axes Rate = 6.0659 A/sec Drift is low enough after shot 1 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.01 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.03 nm/sec Defocus = -1.384 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.21.19_batch_tomo_14_27_010_-20.0.tif Saved Z = 10, -20.00 degrees Mean of buffer A = 121.9 In SetExposureForMean 120, change by a factor of 0.9837 would require too small a change in exposure time Image shift -0.123 0.148 IS units -522.4 193.8 unbinned pixels; need stage -0.051 -0.203 if reset Tilting to -22 7.5 -0.8 unbinned pixels; 1.1 2.4 nm along two shift axes; 0.0 2.7 nm on specimen axes Rate = 13.24971 A/sec Drift is low enough after shot 1 Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.16 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.13 nm/sec Defocus = -1.332 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.22.17_batch_tomo_14_27_011_-22.0.tif Saved Z = 11, -22.00 degrees Mean of buffer A = 120.8 In SetExposureForMean 120, change by a factor of 0.9927 would require too small a change in exposure time Image shift -0.124 0.156 IS units -545.1 190.5 unbinned pixels; need stage -0.049 -0.215 if reset Tilting to 2 Correcting tilt backlash 2.00 degrees -8.9 3.1 unbinned pixels; 2.1 2.6 nm along two shift axes; -0.8 -3.2 nm on specimen axes Rate = 16.51515 A/sec -8.6 2.3 unbinned pixels; 1.8 2.6 nm along two shift axes; -0.5 -3.1 nm on specimen axes Rate = 15.63694 A/sec -6.3 1.9 unbinned pixels; 1.4 1.8 nm along two shift axes; -0.5 -2.3 nm on specimen axes Rate = 11.4771 A/sec Drift is low enough after shot 3 Measured defocus = -3.64 microns changed by -0.11 to target drift = 0.14 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -2.220 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.23.33_batch_tomo_14_27_012_2.0.tif Saved Z = 12, 2.00 degrees Mean of buffer A = 120.7 In SetExposureForMean 120, change by a factor of 0.9944 would require too small a change in exposure time Image shift -0.145 0.186 IS units -646.1 216.5 unbinned pixels; need stage -0.055 -0.236 if reset Tilting to 4 Correcting tilt backlash 8.5 -4.6 unbinned pixels; 2.5 2.3 nm along two shift axes; 1.4 3.1 nm on specimen axes Rate = 16.94386 A/sec 7.4 1.9 unbinned pixels; 0.2 2.7 nm along two shift axes; -0.9 2.5 nm on specimen axes Rate = 13.4071 A/sec Drift is low enough after shot 2 Measured defocus = -3.67 microns changed by -0.08 to target drift = 0.08 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.05 nm/sec Defocus = -2.322 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.24.39_batch_tomo_14_27_013_4.0.tif Saved Z = 13, 4.00 degrees Mean of buffer A = 119.1 In SetExposureForMean 120, change by a factor of 1.0073 would require too small a change in exposure time Image shift -0.126 0.160 IS units -558.2 190.6 unbinned pixels; need stage -0.048 -0.204 if reset Tilting to -24 -24.00 degrees -1.0 -0.9 unbinned pixels; 0.2 0.5 nm along two shift axes; 0.4 -0.3 nm on specimen axes Rate = 2.4845 A/sec Drift is low enough after shot 1 Measured defocus = -3.84 microns changed by 0.09 to target drift = 0.03 nm/sec Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.01 nm/sec Defocus = -1.209 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.25.41_batch_tomo_14_27_014_-24.0.tif Saved Z = 14, -24.00 degrees Mean of buffer A = 119.4 In SetExposureForMean 120, change by a factor of 1.0042 would require too small a change in exposure time Image shift -0.113 0.109 IS units -406.8 204.6 unbinned pixels; need stage -0.058 -0.165 if reset Tilting to -26 8.6 -1.1 unbinned pixels; 1.4 2.7 nm along two shift axes; 0.1 3.0 nm on specimen axes Rate = 15.16212 A/sec 10.3 -0.2 unbinned pixels; 1.3 3.4 nm along two shift axes; -0.2 3.6 nm on specimen axes Rate = 18.02431 A/sec 2.8 -0.4 unbinned pixels; 0.5 0.9 nm along two shift axes; 0.1 1.0 nm on specimen axes Rate = 4.96446 A/sec Drift is low enough after shot 3 Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.08 nm/sec Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.05 nm/sec Defocus = -1.201 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.26.55_batch_tomo_14_27_015_-26.0.tif Saved Z = 15, -26.00 degrees Mean of buffer A = 117.7 In SetExposureForMean 120, change by a factor of 1.0190 would require too small a change in exposure time Image shift -0.095 0.077 IS units -302.7 186.2 unbinned pixels; need stage -0.055 -0.126 if reset Tilting to 6 Correcting tilt backlash 6.00 degrees -31.2 7.5 unbinned pixels; 6.1 9.4 nm along two shift axes; -1.7 -11.2 nm on specimen axes Rate = 56.19736 A/sec -23.5 7.9 unbinned pixels; 5.4 6.8 nm along two shift axes; -2.1 -8.5 nm on specimen axes Rate = 43.4622 A/sec -13.4 3.9 unbinned pixels; 2.8 4.0 nm along two shift axes; -0.9 -4.8 nm on specimen axes Rate = 24.45329 A/sec -7.1 2.8 unbinned pixels; 1.8 2.0 nm along two shift axes; -0.8 -2.6 nm on specimen axes Rate = 13.31698 A/sec Drift is low enough after shot 4 Measured defocus = -3.66 microns changed by -0.09 to target drift = 0.16 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.15 nm/sec Defocus = -2.412 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.28.16_batch_tomo_14_27_016_6.0.tif Saved Z = 16, 6.00 degrees Mean of buffer A = 117.3 In SetExposureForMean 120, change by a factor of 1.0231 would require too small a change in exposure time Image shift -0.178 0.209 IS units -742.1 285.6 unbinned pixels; need stage -0.075 -0.273 if reset Tilting to 8 Correcting tilt backlash 11.1 -6.4 unbinned pixels; 3.4 2.9 nm along two shift axes; 1.9 4.1 nm on specimen axes Rate = 22.47221 A/sec 12.1 2.8 unbinned pixels; 0.5 4.3 nm along two shift axes; -1.4 4.2 nm on specimen axes Rate = 21.82373 A/sec 9.0 1.5 unbinned pixels; 0.6 3.2 nm along two shift axes; -0.8 3.1 nm on specimen axes Rate = 16.05997 A/sec 6.2 0.9 unbinned pixels; 0.4 2.1 nm along two shift axes; -0.5 2.1 nm on specimen axes Rate = 10.91973 A/sec Drift is low enough after shot 4 Measured defocus = -3.66 microns changed by -0.09 to target drift = 0.11 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -2.512 um Dewars are NOT busy filling 21 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.29.34_batch_tomo_14_27_017_8.0.tif Saved Z = 17, 8.00 degrees Mean of buffer A = 115.9 In SetExposureForMean 120, exposure time changed to 2.6400 Image shift -0.137 0.128 IS units -480.9 250.1 unbinned pixels; need stage -0.071 -0.180 if reset Tilting to -28 -28.00 degrees -0.4 -0.7 unbinned pixels; 0.2 0.2 nm along two shift axes; 0.3 -0.1 nm on specimen axes Rate = 1.45823 A/sec Drift is low enough after shot 1 Measured defocus = -3.86 microns changed by 0.11 to target drift = 0.03 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.03 nm/sec Defocus = -1.089 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.30.36_batch_tomo_14_27_018_-28.0.tif Saved Z = 18, -28.00 degrees Mean of buffer A = 121.6 In SetExposureForMean 120, change by a factor of 0.9866 would require too small a change in exposure time Image shift -0.096 0.036 IS units -193.0 227.1 unbinned pixels; need stage -0.072 -0.086 if reset Tilting to -30 6.4 -2.7 unbinned pixels; 1.6 1.8 nm along two shift axes; 0.8 2.3 nm on specimen axes Rate = 12.13322 A/sec Drift is low enough after shot 1 Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.14 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.08 nm/sec Defocus = -1.044 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.31.35_batch_tomo_14_27_019_-30.0.tif Saved Z = 19, -30.00 degrees Mean of buffer A = 118.7 In SetExposureForMean 120, change by a factor of 1.0107 would require too small a change in exposure time Image shift -0.073 -0.006 IS units -54.4 204.8 unbinned pixels; need stage -0.069 -0.030 if reset Tilting to 10 Correcting tilt backlash 10.00 degrees -44.0 9.0 unbinned pixels; 8.1 13.5 nm along two shift axes; -1.8 -15.7 nm on specimen axes Rate = 78.71604 A/sec -23.3 8.4 unbinned pixels; 5.5 6.7 nm along two shift axes; -2.2 -8.4 nm on specimen axes Rate = 43.44014 A/sec -12.9 3.4 unbinned pixels; 2.6 3.8 nm along two shift axes; -0.8 -4.6 nm on specimen axes Rate = 23.31889 A/sec -10.1 1.3 unbinned pixels; 1.6 3.2 nm along two shift axes; -0.2 -3.6 nm on specimen axes Rate = 17.88452 A/sec -5.9 2.6 unbinned pixels; 1.5 1.7 nm along two shift axes; -0.7 -2.2 nm on specimen axes Rate = 11.32351 A/sec Drift is low enough after shot 5 Measured defocus = -3.67 microns changed by -0.08 to target drift = 0.11 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.10 nm/sec Defocus = -2.589 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.33.02_batch_tomo_14_27_020_10.0.tif Saved Z = 20, 10.00 degrees Mean of buffer A = 119.2 In SetExposureForMean 120, change by a factor of 1.0065 would require too small a change in exposure time Image shift -0.192 0.213 IS units -767.3 320.3 unbinned pixels; need stage -0.087 -0.286 if reset Tilting to 12 Correcting tilt backlash 21.9 -5.6 unbinned pixels; 4.4 6.6 nm along two shift axes; 1.3 7.9 nm on specimen axes Rate = 39.68135 A/sec 17.0 4.0 unbinned pixels; 0.7 6.1 nm along two shift axes; -1.9 5.8 nm on specimen axes Rate = 30.6613 A/sec 8.5 1.9 unbinned pixels; 0.4 3.0 nm along two shift axes; -0.9 2.9 nm on specimen axes Rate = 15.34142 A/sec 5.7 1.5 unbinned pixels; 0.2 2.1 nm along two shift axes; -0.7 2.0 nm on specimen axes Rate = 10.41622 A/sec Drift is low enough after shot 4 Measured defocus = -3.68 microns changed by -0.07 to target drift = 0.07 nm/sec Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.04 nm/sec Defocus = -2.662 um Dewars are NOT busy filling 22 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.34.19_batch_tomo_14_27_021_12.0.tif Saved Z = 21, 12.00 degrees Mean of buffer A = 116.7 In SetExposureForMean 120, exposure time changed to 2.7600 Image shift -0.139 0.101 IS units -409.5 283.0 unbinned pixels; need stage -0.085 -0.157 if reset Tilting to -32 -32.00 degrees 0.3 -0.8 unbinned pixels; 0.3 0.0 nm along two shift axes; 0.3 0.1 nm on specimen axes Rate = 1.40855 A/sec Drift is low enough after shot 1 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.06 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.04 nm/sec Defocus = -0.963 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.35.22_batch_tomo_14_27_022_-32.0.tif Saved Z = 22, -32.00 degrees Mean of buffer A = 121.9 In SetExposureForMean 120, change by a factor of 0.9838 would require too small a change in exposure time Image shift -0.057 -0.070 IS units 133.2 220.9 unbinned pixels; need stage -0.080 0.047 if reset Tilting to -34 5.6 -2.5 unbinned pixels; 1.5 1.5 nm along two shift axes; 0.7 2.0 nm on specimen axes Rate = 10.68947 A/sec Drift is low enough after shot 1 Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.10 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.14 nm/sec Defocus = -0.927 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.36.21_batch_tomo_14_27_023_-34.0.tif Saved Z = 23, -34.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0045 would require too small a change in exposure time Image shift -0.029 -0.122 IS units 302.1 194.7 unbinned pixels; need stage -0.076 0.121 if reset Tilting to 14 Correcting tilt backlash 14.00 degrees -39.3 8.9 unbinned pixels; 7.6 11.9 nm along two shift axes; -1.9 -14.1 nm on specimen axes Rate = 70.66776 A/sec -12.6 3.5 unbinned pixels; 2.7 3.8 nm along two shift axes; -0.9 -4.5 nm on specimen axes Rate = 22.99923 A/sec -10.8 4.5 unbinned pixels; 2.7 3.0 nm along two shift axes; -1.2 -3.9 nm on specimen axes Rate = 20.41713 A/sec -4.6 2.6 unbinned pixels; 1.4 1.2 nm along two shift axes; -0.8 -1.7 nm on specimen axes Rate = 9.2266 A/sec Drift is low enough after shot 4 Measured defocus = -3.65 microns changed by -0.10 to target drift = 0.11 nm/sec Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.07 nm/sec Defocus = -2.797 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.37.43_batch_tomo_14_27_024_14.0.tif Saved Z = 24, 14.00 degrees Mean of buffer A = 119.6 In SetExposureForMean 120, change by a factor of 1.0035 would require too small a change in exposure time Image shift -0.181 0.174 IS units -650.8 326.2 unbinned pixels; need stage -0.092 -0.248 if reset Tilting to 16 Correcting tilt backlash 24.2 -1.8 unbinned pixels; 3.4 7.8 nm along two shift axes; -0.1 8.5 nm on specimen axes Rate = 42.48683 A/sec 9.2 4.7 unbinned pixels; 0.5 3.6 nm along two shift axes; -1.9 3.1 nm on specimen axes Rate = 18.17579 A/sec 4.8 1.6 unbinned pixels; 0.0 1.8 nm along two shift axes; -0.7 1.6 nm on specimen axes Rate = 8.85147 A/sec Drift is low enough after shot 3 Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.08 nm/sec Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.06 nm/sec Defocus = -2.824 um Dewars are NOT busy filling 23 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.38.55_batch_tomo_14_27_025_16.0.tif Saved Z = 25, 16.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, exposure time changed to 2.8800 Image shift -0.126 0.047 IS units -251.6 297.1 unbinned pixels; need stage -0.095 -0.103 if reset Tilting to -36 -36.00 degrees 2.8 -2.7 unbinned pixels; 1.2 0.6 nm along two shift axes; 0.9 1.1 nm on specimen axes Rate = 6.8443 A/sec Drift is low enough after shot 1 Measured defocus = -3.85 microns changed by 0.10 to target drift = 0.08 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.05 nm/sec Defocus = -0.809 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.40.03_batch_tomo_14_27_026_-36.0.tif Saved Z = 26, -36.00 degrees Mean of buffer A = 122.2 In SetExposureForMean 120, change by a factor of 0.9796 would require too small a change in exposure time Image shift 0.001 -0.219 IS units 593.6 205.4 unbinned pixels; need stage -0.089 0.251 if reset Tilting to -38 7.2 -5.8 unbinned pixels; 2.8 1.7 nm along two shift axes; 1.8 2.7 nm on specimen axes Rate = 16.19291 A/sec 5.9 3.3 unbinned pixels; 0.4 2.3 nm along two shift axes; -1.3 2.0 nm on specimen axes Rate = 11.88042 A/sec Drift is low enough after shot 2 Measured defocus = -3.80 microns changed by 0.05 to target drift = 0.10 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.758 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.41.08_batch_tomo_14_27_027_-38.0.tif Saved Z = 27, -38.00 degrees Mean of buffer A = 119.0 In SetExposureForMean 120, change by a factor of 1.0051 would require too small a change in exposure time Image shift 0.034 -0.281 IS units 794.9 173.9 unbinned pixels; need stage -0.084 0.350 if reset Tilting to 18 Correcting tilt backlash 18.00 degrees -42.0 10.3 unbinned pixels; 8.3 12.7 nm along two shift axes; -2.3 -15.0 nm on specimen axes Rate = 75.73638 A/sec -17.8 7.0 unbinned pixels; 4.4 5.0 nm along two shift axes; -1.9 -6.5 nm on specimen axes Rate = 33.50897 A/sec -5.5 3.2 unbinned pixels; 1.7 1.4 nm along two shift axes; -1.0 -2.0 nm on specimen axes Rate = 11.1128 A/sec Drift is low enough after shot 3 Measured defocus = -3.60 microns changed by -0.15 to target drift = 0.13 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.11 nm/sec Defocus = -2.995 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.42.24_batch_tomo_14_27_028_18.0.tif Saved Z = 28, 18.00 degrees Mean of buffer A = 118.6 In SetExposureForMean 120, change by a factor of 1.0119 would require too small a change in exposure time Image shift -0.146 0.079 IS units -356.5 321.9 unbinned pixels; need stage -0.100 -0.143 if reset Tilting to 20 Correcting tilt backlash 17.5 -1.4 unbinned pixels; 2.5 5.6 nm along two shift axes; -0.0 6.2 nm on specimen axes Rate = 30.86367 A/sec 16.6 5.3 unbinned pixels; 0.2 6.1 nm along two shift axes; -2.4 5.6 nm on specimen axes Rate = 30.57294 A/sec 11.0 2.3 unbinned pixels; 0.5 3.9 nm along two shift axes; -1.1 3.8 nm on specimen axes Rate = 19.79579 A/sec 6.1 1.4 unbinned pixels; 0.3 2.2 nm along two shift axes; -0.7 2.1 nm on specimen axes Rate = 10.93296 A/sec Drift is low enough after shot 4 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.07 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.05 nm/sec Defocus = -3.038 um Dewars are NOT busy filling 24 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.43.42_batch_tomo_14_27_029_20.0.tif Saved Z = 29, 20.00 degrees Mean of buffer A = 115.2 In SetExposureForMean 120, exposure time changed to 3.0000 Image shift -0.086 -0.068 IS units 97.9 299.5 unbinned pixels; need stage -0.106 0.026 if reset Tilting to -40 -40.00 degrees 2.5 -1.6 unbinned pixels; 0.8 0.7 nm along two shift axes; 0.5 0.9 nm on specimen axes Rate = 5.21582 A/sec Drift is low enough after shot 1 Measured defocus = -3.86 microns changed by 0.11 to target drift = 0.09 nm/sec Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.08 nm/sec Defocus = -0.678 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.44.46_batch_tomo_14_27_030_-40.0.tif Saved Z = 30, -40.00 degrees Mean of buffer A = 120.4 In SetExposureForMean 120, change by a factor of 0.9938 would require too small a change in exposure time Image shift 0.060 -0.368 IS units 1056.7 186.4 unbinned pixels; need stage -0.097 0.480 if reset Tilting to -42 4.3 0.0 unbinned pixels; 0.5 1.4 nm along two shift axes; -0.1 1.5 nm on specimen axes Rate = 7.55697 A/sec Drift is low enough after shot 1 Measured defocus = -3.81 microns changed by 0.06 to target drift = 0.08 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.610 um Dewars are NOT busy filling 25 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.45.45_batch_tomo_14_27_031_-42.0.tif Saved Z = 31, -42.00 degrees Mean of buffer A = 116.3 In SetExposureForMean 120, exposure time changed to 3.1200 Image shift 0.078 -0.399 IS units 1156.5 168.1 unbinned pixels; need stage -0.093 0.543 if reset Tilting to 22 Correcting tilt backlash 22.00 degrees -11.4 5.2 unbinned pixels; 3.1 3.2 nm along two shift axes; -1.5 -4.2 nm on specimen axes Rate = 22.00831 A/sec -2.9 1.4 unbinned pixels; 0.8 0.8 nm along two shift axes; -0.4 -1.1 nm on specimen axes Rate = 5.67872 A/sec Drift is low enough after shot 2 Measured defocus = -3.61 microns changed by -0.14 to target drift = 0.16 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.13 nm/sec Defocus = -3.167 um Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.46.56_batch_tomo_14_27_032_22.0.tif Saved Z = 32, 22.00 degrees Mean of buffer A = 120.8 In SetExposureForMean 120, change by a factor of 0.9935 would require too small a change in exposure time Image shift -0.102 -0.041 IS units 11.1 316.9 unbinned pixels; need stage -0.109 -0.007 if reset Tilting to 24 Correcting tilt backlash -2.2 -4.9 unbinned pixels; 1.4 1.3 nm along two shift axes; 1.8 -0.6 nm on specimen axes Rate = 9.45928 A/sec Drift is low enough after shot 1 Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.16 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.08 nm/sec Defocus = -3.160 um Dewars are NOT busy filling 26 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.47.58_batch_tomo_14_27_033_24.0.tif Saved Z = 33, 24.00 degrees Mean of buffer A = 116.9 In SetExposureForMean 120, exposure time changed to 3.2400 Image shift -0.050 -0.183 IS units 447.1 309.7 unbinned pixels; need stage -0.120 0.162 if reset Tilting to -44 -44.00 degrees 4.5 -7.0 unbinned pixels; 2.8 0.7 nm along two shift axes; 2.3 1.8 nm on specimen axes Rate = 14.63225 A/sec 3.9 3.4 unbinned pixels; 0.7 1.7 nm along two shift axes; -1.3 1.3 nm on specimen axes Rate = 9.15718 A/sec Drift is low enough after shot 2 Measured defocus = -3.83 microns changed by 0.08 to target drift = 0.10 nm/sec Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.07 nm/sec Defocus = -0.555 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.49.08_batch_tomo_14_27_034_-44.0.tif Saved Z = 34, -44.00 degrees Mean of buffer A = 120.9 In SetExposureForMean 120, change by a factor of 0.9886 would require too small a change in exposure time Image shift 0.114 -0.497 IS units 1458.6 161.1 unbinned pixels; need stage -0.100 0.710 if reset Tilting to -46 5.4 -6.7 unbinned pixels; 2.8 1.0 nm along two shift axes; 2.2 2.1 nm on specimen axes Rate = 15.07287 A/sec 4.3 2.7 unbinned pixels; 0.4 1.8 nm along two shift axes; -1.1 1.4 nm on specimen axes Rate = 8.97918 A/sec Drift is low enough after shot 2 Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.06 nm/sec Measured defocus = -3.81 microns changed by 0.06 to target drift = 0.06 nm/sec Defocus = -0.521 um Dewars are NOT busy filling 27 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.50.13_batch_tomo_14_27_035_-46.0.tif Saved Z = 35, -46.00 degrees Mean of buffer A = 116.2 In SetExposureForMean 120, exposure time changed to 3.3600 Image shift 0.153 -0.571 IS units 1698.0 126.6 unbinned pixels; need stage -0.096 0.858 if reset Tilting to 26 Correcting tilt backlash 26.00 degrees -11.0 1.8 unbinned pixels; 1.9 3.4 nm along two shift axes; -0.3 -3.9 nm on specimen axes Rate = 19.59172 A/sec -7.9 2.6 unbinned pixels; 1.8 2.3 nm along two shift axes; -0.7 -2.9 nm on specimen axes Rate = 14.5666 A/sec -8.6 1.0 unbinned pixels; 1.3 2.7 nm along two shift axes; -0.1 -3.1 nm on specimen axes Rate = 15.25776 A/sec -5.1 1.6 unbinned pixels; 1.1 1.5 nm along two shift axes; -0.4 -1.8 nm on specimen axes Rate = 9.27208 A/sec Drift is low enough after shot 4 Measured defocus = -3.64 microns changed by -0.11 to target drift = 0.06 nm/sec Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.14 nm/sec Defocus = -3.266 um Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.51.37_batch_tomo_14_27_036_26.0.tif Saved Z = 36, 26.00 degrees Mean of buffer A = 120.7 In SetExposureForMean 120, change by a factor of 0.9949 would require too small a change in exposure time Image shift -0.066 -0.140 IS units 314.9 312.4 unbinned pixels; need stage -0.117 0.113 if reset Tilting to 28 Correcting tilt backlash -10.4 -7.0 unbinned pixels; 1.1 4.3 nm along two shift axes; 2.8 -3.4 nm on specimen axes Rate = 22.12583 A/sec -5.5 7.0 unbinned pixels; 3.0 1.0 nm along two shift axes; -2.3 -2.1 nm on specimen axes Rate = 15.54065 A/sec -2.0 1.4 unbinned pixels; 0.7 0.5 nm along two shift axes; -0.4 -0.7 nm on specimen axes Rate = 4.27118 A/sec Drift is low enough after shot 3 Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.15 nm/sec Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.12 nm/sec Defocus = -3.279 um Dewars are NOT busy filling 28 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.52.51_batch_tomo_14_27_037_28.0.tif Saved Z = 37, 28.00 degrees Mean of buffer A = 115.4 In SetExposureForMean 120, exposure time changed to 3.4800 Image shift 0.004 -0.307 IS units 836.7 280.9 unbinned pixels; need stage -0.122 0.325 if reset Tilting to -48 -48.00 degrees 6.3 -6.4 unbinned pixels; 2.9 1.3 nm along two shift axes; 2.1 2.4 nm on specimen axes Rate = 15.78883 A/sec 5.8 2.6 unbinned pixels; 0.2 2.2 nm along two shift axes; -1.1 2.0 nm on specimen axes Rate = 11.20083 A/sec Drift is low enough after shot 2 Measured defocus = -3.82 microns changed by 0.07 to target drift = 0.10 nm/sec Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.09 nm/sec Defocus = -0.486 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.54.03_batch_tomo_14_27_038_-48.0.tif Saved Z = 38, -48.00 degrees Mean of buffer A = 120.1 In SetExposureForMean 120, change by a factor of 0.9944 would require too small a change in exposure time Image shift 0.191 -0.652 IS units 1955.4 102.0 unbinned pixels; need stage -0.095 1.028 if reset Tilting to -50 4.9 -2.4 unbinned pixels; 1.4 1.3 nm along two shift axes; 0.7 1.8 nm on specimen axes Rate = 9.60153 A/sec Drift is low enough after shot 1 Measured defocus = -3.70 microns changed by -0.05 to target drift = 0.12 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.05 nm/sec Defocus = -0.526 um Dewars are NOT busy filling 29 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.55.04_batch_tomo_14_27_039_-50.0.tif Saved Z = 39, -50.00 degrees Mean of buffer A = 113.9 In SetExposureForMean 120, exposure time changed to 3.6000 Image shift 0.218 -0.708 IS units 2131.1 81.2 unbinned pixels; need stage -0.094 1.168 if reset Tilting to 30 Correcting tilt backlash 30.00 degrees -10.1 1.6 unbinned pixels; 1.7 3.1 nm along two shift axes; -0.3 -3.6 nm on specimen axes Rate = 17.91641 A/sec -9.1 2.6 unbinned pixels; 1.9 2.7 nm along two shift axes; -0.6 -3.3 nm on specimen axes Rate = 16.63013 A/sec -5.4 1.4 unbinned pixels; 1.1 1.6 nm along two shift axes; -0.3 -2.0 nm on specimen axes Rate = 9.85618 A/sec Drift is low enough after shot 3 Measured defocus = -3.66 microns changed by -0.09 to target drift = 0.21 nm/sec Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.12 nm/sec Defocus = -3.388 um Dewars are NOT busy filling 30 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.56.25_batch_tomo_14_27_040_30.0.tif Saved Z = 40, 30.00 degrees Mean of buffer A = 117.8 In SetExposureForMean 120, exposure time changed to 3.7200 Image shift -0.012 -0.267 IS units 712.2 287.8 unbinned pixels; need stage -0.121 0.280 if reset Tilting to 32 Correcting tilt backlash -11.2 -4.9 unbinned pixels; 0.3 4.3 nm along two shift axes; 2.1 -3.8 nm on specimen axes Rate = 21.58116 A/sec -8.0 3.3 unbinned pixels; 2.0 2.2 nm along two shift axes; -0.9 -2.9 nm on specimen axes Rate = 15.17738 A/sec -1.6 1.9 unbinned pixels; 0.8 0.3 nm along two shift axes; -0.6 -0.6 nm on specimen axes Rate = 4.29982 A/sec Drift is low enough after shot 3 Measured defocus = -3.73 microns changed by -0.02 to target drift = 0.09 nm/sec Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.08 nm/sec Defocus = -3.420 um Dewars are NOT busy filling 31 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.57.39_batch_tomo_14_27_041_32.0.tif Saved Z = 41, 32.00 degrees Mean of buffer A = 115.5 In SetExposureForMean 120, exposure time changed to 3.8400 Image shift 0.036 -0.370 IS units 1038.4 254.1 unbinned pixels; need stage -0.119 0.423 if reset Tilting to -52 -52.00 degrees 8.1 -6.2 unbinned pixels; 3.0 1.9 nm along two shift axes; 1.9 3.0 nm on specimen axes Rate = 17.90321 A/sec 6.9 1.9 unbinned pixels; 0.2 2.5 nm along two shift axes; -0.9 2.4 nm on specimen axes Rate = 12.54399 A/sec Drift is low enough after shot 2 Measured defocus = -3.89 microns changed by 0.14 to target drift = 0.20 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.08 nm/sec Defocus = -0.374 um Dewars are NOT busy filling 32 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.58.52_batch_tomo_14_27_042_-52.0.tif Saved Z = 42, -52.00 degrees Mean of buffer A = 119.0 In SetExposureForMean 120, change by a factor of 1.0005 would require too small a change in exposure time Image shift 0.255 -0.773 IS units 2346.6 42.1 unbinned pixels; need stage -0.087 1.345 if reset Tilting to -54 3.5 -0.7 unbinned pixels; 0.7 1.1 nm along two shift axes; 0.1 1.3 nm on specimen axes Rate = 6.33729 A/sec Drift is low enough after shot 1 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.08 nm/sec Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.08 nm/sec Defocus = -0.456 um Dewars are NOT busy filling 32 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_08.59.53_batch_tomo_14_27_043_-54.0.tif Saved Z = 43, -54.00 degrees Mean of buffer A = 112.2 In SetExposureForMean 120, exposure time changed to 4.0800 Image shift 0.274 -0.828 IS units 2512.7 41.7 unbinned pixels; need stage -0.092 1.509 if reset Tilting to 34 Correcting tilt backlash 34.00 degrees -11.0 3.3 unbinned pixels; 2.4 3.3 nm along two shift axes; -0.8 -4.0 nm on specimen axes Rate = 20.15487 A/sec -11.8 3.7 unbinned pixels; 2.6 3.5 nm along two shift axes; -0.9 -4.3 nm on specimen axes Rate = 21.70714 A/sec -7.5 2.1 unbinned pixels; 1.6 2.2 nm along two shift axes; -0.5 -2.7 nm on specimen axes Rate = 13.59905 A/sec Drift is low enough after shot 3 Measured defocus = -3.61 microns changed by -0.14 to target drift = 0.19 nm/sec Measured defocus = -3.75 microns changed by 0.00 to target drift = 0.16 nm/sec Defocus = -3.559 um Dewars are NOT busy filling 34 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.01.14_batch_tomo_14_27_044_34.0.tif Saved Z = 44, 34.00 degrees Mean of buffer A = 119.5 In SetExposureForMean 120, change by a factor of 1.0062 would require too small a change in exposure time Image shift 0.019 -0.338 IS units 933.5 269.5 unbinned pixels; need stage -0.121 0.388 if reset Tilting to 36 Correcting tilt backlash -12.1 -7.4 unbinned pixels; 1.0 4.9 nm along two shift axes; 3.0 -4.0 nm on specimen axes Rate = 25.03848 A/sec -6.1 5.5 unbinned pixels; 2.5 1.4 nm along two shift axes; -1.7 -2.3 nm on specimen axes Rate = 14.40039 A/sec -2.4 1.1 unbinned pixels; 0.7 0.7 nm along two shift axes; -0.3 -0.9 nm on specimen axes Rate = 4.66099 A/sec Drift is low enough after shot 3 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.06 nm/sec Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.11 nm/sec Defocus = -3.585 um Dewars are NOT busy filling 34 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.02.30_batch_tomo_14_27_045_36.0.tif Saved Z = 45, 36.00 degrees Mean of buffer A = 111.6 In SetExposureForMean 120, exposure time changed to 4.4400 Image shift 0.080 -0.468 IS units 1347.5 226.5 unbinned pixels; need stage -0.119 0.580 if reset Tilting to -56 -56.00 degrees 9.7 -5.3 unbinned pixels; 2.9 2.6 nm along two shift axes; 1.6 3.6 nm on specimen axes Rate = 19.32165 A/sec 5.8 1.9 unbinned pixels; 0.0 2.1 nm along two shift axes; -0.8 2.0 nm on specimen axes Rate = 10.69655 A/sec Drift is low enough after shot 2 Measured defocus = -3.83 microns changed by 0.08 to target drift = 0.11 nm/sec Measured defocus = -3.70 microns changed by -0.05 to target drift = 0.12 nm/sec Defocus = -0.430 um Dewars are NOT busy filling 37 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.03.43_batch_tomo_14_27_046_-56.0.tif Saved Z = 46, -56.00 degrees Mean of buffer A = 120.5 In SetExposureForMean 120, change by a factor of 0.9934 would require too small a change in exposure time Image shift 0.308 -0.899 IS units 2739.0 17.8 unbinned pixels; need stage -0.090 1.731 if reset Tilting to -58 5.0 0.8 unbinned pixels; 0.3 1.7 nm along two shift axes; -0.4 1.7 nm on specimen axes Rate = 8.83517 A/sec Drift is low enough after shot 1 Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.05 nm/sec Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.07 nm/sec Defocus = -0.458 um Dewars are NOT busy filling 37 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.04.43_batch_tomo_14_27_047_-58.0.tif Saved Z = 47, -58.00 degrees Mean of buffer A = 112.6 In SetExposureForMean 120, exposure time changed to 4.6800 Image shift 0.313 -0.922 IS units 2806.7 25.1 unbinned pixels; need stage -0.095 1.871 if reset Tilting to 38 Correcting tilt backlash 38.00 degrees -17.8 -1.4 unbinned pixels; 1.6 6.1 nm along two shift axes; 1.0 -6.2 nm on specimen axes Rate = 31.36122 A/sec -10.9 6.0 unbinned pixels; 3.3 2.9 nm along two shift axes; -1.8 -4.0 nm on specimen axes Rate = 21.80524 A/sec -7.4 2.7 unbinned pixels; 1.7 2.1 nm along two shift axes; -0.7 -2.7 nm on specimen axes Rate = 13.78302 A/sec Drift is low enough after shot 3 Measured defocus = -3.62 microns changed by -0.13 to target drift = 0.18 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.17 nm/sec Defocus = -3.705 um Dewars are NOT busy filling 39 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.06.04_batch_tomo_14_27_048_38.0.tif Saved Z = 48, 38.00 degrees Mean of buffer A = 119.3 In SetExposureForMean 120, change by a factor of 1.0094 would require too small a change in exposure time Image shift 0.082 -0.475 IS units 1368.0 229.0 unbinned pixels; need stage -0.121 0.604 if reset Tilting to 40 Correcting tilt backlash -11.0 -8.2 unbinned pixels; 1.5 4.6 nm along two shift axes; 3.2 -3.6 nm on specimen axes Rate = 24.27176 A/sec -5.6 4.8 unbinned pixels; 2.3 1.3 nm along two shift axes; -1.5 -2.1 nm on specimen axes Rate = 12.99181 A/sec Drift is low enough after shot 2 Measured defocus = -3.74 microns changed by -0.01 to target drift = 0.13 nm/sec Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.07 nm/sec Defocus = -3.757 um Dewars are NOT busy filling 39 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.07.12_batch_tomo_14_27_049_40.0.tif Saved Z = 49, 40.00 degrees Mean of buffer A = 109.8 In SetExposureForMean 120, exposure time changed to 5.1600 Image shift 0.134 -0.586 IS units 1719.0 191.8 unbinned pixels; need stage -0.119 0.785 if reset Tilting to -60 -60.00 degrees 7.5 -2.5 unbinned pixels; 1.7 2.2 nm along two shift axes; 0.7 2.7 nm on specimen axes Rate = 13.90315 A/sec Drift is low enough after shot 1 Measured defocus = -3.85 microns changed by 0.10 to target drift = 0.11 nm/sec Measured defocus = -3.69 microns changed by -0.06 to target drift = 0.10 nm/sec Defocus = -0.421 um Dewars are NOT busy filling 43 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.08.19_batch_tomo_14_27_050_-60.0.tif Saved Z = 50, -60.00 degrees Mean of buffer A = 121.1 In SetExposureForMean 120, exposure time changed to 5.0400 Image shift 0.334 -0.969 IS units 2955.1 12.8 unbinned pixels; need stage -0.095 2.089 if reset Tilting to -62 2.1 0.6 unbinned pixels; 0.0 0.8 nm along two shift axes; -0.3 0.7 nm on specimen axes Rate = 3.76043 A/sec Drift is low enough after shot 1 Measured defocus = -3.77 microns changed by 0.02 to target drift = 0.04 nm/sec Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.04 nm/sec Defocus = -0.445 um Dewars are NOT busy filling 42 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.09.21_batch_tomo_14_27_051_-62.0.tif Saved Z = 51, -62.00 degrees Mean of buffer A = 107.7 In SetExposureForMean 120, exposure time changed to 5.5200 Image shift 0.346 -0.996 IS units 3040.4 5.0 unbinned pixels; need stage -0.095 2.290 if reset Tilting to 42 Correcting tilt backlash 42.00 degrees -23.8 -2.9 unbinned pixels; 1.8 8.2 nm along two shift axes; 1.7 -8.3 nm on specimen axes Rate = 42.14362 A/sec -12.5 4.9 unbinned pixels; 3.1 3.5 nm along two shift axes; -1.3 -4.5 nm on specimen axes Rate = 23.51479 A/sec -7.8 2.5 unbinned pixels; 1.7 2.3 nm along two shift axes; -0.6 -2.8 nm on specimen axes Rate = 14.29836 A/sec -7.9 1.7 unbinned pixels; 1.5 2.4 nm along two shift axes; -0.4 -2.8 nm on specimen axes Rate = 14.15861 A/sec -7.0 2.0 unbinned pixels; 1.5 2.1 nm along two shift axes; -0.5 -2.5 nm on specimen axes Rate = 12.8245 A/sec Drift is low enough after shot 5 Measured defocus = -3.60 microns changed by -0.15 to target drift = 0.15 nm/sec Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.19 nm/sec Defocus = -3.865 um Dewars are NOT busy filling 46 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.10.53_batch_tomo_14_27_052_42.0.tif Saved Z = 52, 42.00 degrees Mean of buffer A = 118.8 In SetExposureForMean 120, exposure time changed to 5.6400 Image shift 0.143 -0.600 IS units 1766.2 181.0 unbinned pixels; need stage -0.117 0.832 if reset Tilting to 44 Correcting tilt backlash -10.4 -8.8 unbinned pixels; 1.7 4.5 nm along two shift axes; 3.4 -3.4 nm on specimen axes Rate = 24.01469 A/sec -6.6 4.3 unbinned pixels; 2.2 1.7 nm along two shift axes; -1.3 -2.4 nm on specimen axes Rate = 13.7829 A/sec Drift is low enough after shot 2 Measured defocus = -3.75 microns changed by -0.00 to target drift = 0.14 nm/sec Measured defocus = -3.76 microns changed by 0.01 to target drift = 0.15 nm/sec Defocus = -3.861 um Dewars are NOT busy filling 47 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.12.03_batch_tomo_14_27_053_44.0.tif Saved Z = 53, 44.00 degrees Mean of buffer A = 108.8 In SetExposureForMean 120, exposure time changed to 6.2400 Image shift 0.196 -0.716 IS units 2132.1 148.8 unbinned pixels; need stage -0.117 1.041 if reset Tilting to -64 -64.00 degrees 4.4 -2.7 unbinned pixels; 1.4 1.1 nm along two shift axes; 0.8 1.6 nm on specimen axes Rate = 8.94521 A/sec Drift is low enough after shot 1 Measured defocus = -3.71 microns changed by -0.04 to target drift = 0.06 nm/sec Measured defocus = -3.83 microns changed by 0.08 to target drift = 0.26 nm/sec Defocus = -0.406 um Dewars are NOT busy filling 52 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.13.12_batch_tomo_14_27_054_-64.0.tif Saved Z = 54, -64.00 degrees Mean of buffer A = 120.4 In SetExposureForMean 120, exposure time changed to 6.1200 Image shift 0.373 -1.058 IS units 3235.1 -8.3 unbinned pixels; need stage -0.097 2.610 if reset Tilting to -66 -0.2 0.8 unbinned pixels; 0.3 0.0 nm along two shift axes; -0.3 -0.1 nm on specimen axes Rate = 1.48605 A/sec Drift is low enough after shot 1 Measured defocus = -3.66 microns changed by -0.09 to target drift = 0.14 nm/sec Measured defocus = -3.72 microns changed by -0.03 to target drift = 0.04 nm/sec Defocus = -0.523 um Dewars are NOT busy filling 51 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.14.15_batch_tomo_14_27_055_-66.0.tif Saved Z = 55, -66.00 degrees Mean of buffer A = 104.9 In SetExposureForMean 120, exposure time changed to 6.8400 Image shift 0.390 -1.094 IS units 3347.7 -21.2 unbinned pixels; need stage -0.096 2.912 if reset Tilting to 46 Correcting tilt backlash 46.00 degrees -22.8 -1.9 unbinned pixels; 2.0 7.8 nm along two shift axes; 1.4 -7.9 nm on specimen axes Rate = 40.14245 A/sec -9.1 5.5 unbinned pixels; 2.9 2.4 nm along two shift axes; -1.6 -3.4 nm on specimen axes Rate = 18.66327 A/sec -8.3 2.3 unbinned pixels; 1.7 2.5 nm along two shift axes; -0.5 -3.0 nm on specimen axes Rate = 15.07435 A/sec -6.8 1.7 unbinned pixels; 1.4 2.1 nm along two shift axes; -0.4 -2.4 nm on specimen axes Rate = 12.32449 A/sec Drift is low enough after shot 4 Measured defocus = -3.61 microns changed by -0.14 to target drift = 0.15 nm/sec Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.15 nm/sec Defocus = -3.968 um Dewars are NOT busy filling 57 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.15.43_batch_tomo_14_27_056_46.0.tif Saved Z = 56, 46.00 degrees Mean of buffer A = 117.5 In SetExposureForMean 120, exposure time changed to 7.0800 Image shift 0.210 -0.734 IS units 2195.3 127.8 unbinned pixels; need stage -0.112 1.111 if reset Tilting to 48 Correcting tilt backlash -9.6 -8.2 unbinned pixels; 1.6 4.2 nm along two shift axes; 3.2 -3.1 nm on specimen axes Rate = 22.35235 A/sec -2.8 2.9 unbinned pixels; 1.3 0.6 nm along two shift axes; -0.9 -1.1 nm on specimen axes Rate = 7.05183 A/sec Drift is low enough after shot 2 Measured defocus = -3.78 microns changed by 0.03 to target drift = 0.07 nm/sec Measured defocus = -3.79 microns changed by 0.04 to target drift = 0.08 nm/sec Defocus = -3.894 um Dewars are NOT busy filling 59 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.16.54_batch_tomo_14_27_057_48.0.tif Saved Z = 57, 48.00 degrees Mean of buffer A = 106.1 In SetExposureForMean 120, exposure time changed to 8.1600 Image shift 0.269 -0.821 IS units 2488.5 49.0 unbinned pixels; need stage -0.093 1.313 if reset Tilting to -68 -68.00 degrees 6.6 -6.5 unbinned pixels; 2.9 1.4 nm along two shift axes; 2.1 2.5 nm on specimen axes Rate = 16.27728 A/sec 3.1 2.8 unbinned pixels; 0.6 1.3 nm along two shift axes; -1.1 1.0 nm on specimen axes Rate = 7.33474 A/sec Drift is low enough after shot 2 Measured defocus = -3.87 microns changed by 0.12 to target drift = 0.05 nm/sec Measured defocus = -3.59 microns changed by -0.16 to target drift = 0.03 nm/sec Defocus = -0.560 um Dewars are NOT busy filling 68 frames were saved to D:\Users\Ben\210112_Titan2_Jans_cells\frames\2021-01-13_09.18.10_batch_tomo_14_27_058_-68.0.tif Saved Z = 58, -68.00 degrees Mean of buffer A = 122.3 In SetExposureForMean 120, exposure time changed to 7.8000 Image shift 0.432 -1.143 IS units 3521.6 -87.4 unbinned pixels; need stage -0.078 3.332 if reset Omitting tilt at -70 Omitting tilt at 50 Setting Eucentric Focus! Measured defocus = 1.35 microns changed by -5.10 to target drift = 0.43 nm/sec