WARNING: Autofocus aborted after 8 iterations and focus returned to starting value (-5.81) because too may iterations have been run. Check focus calibration and/or scope alignment if images and specimen look OK Dewars are NOT busy filling Refrigerant level in dewar 1 is 16.813 Refrigerant level in dewar 2 is 49.658 Opened new file X:\Luke_20191213\s1_3part.st STARTING TILT SERIES WITH AUTOMATIC TERMINATION AFTER ERRORS. Trimming A by 20 20, reference by 45 45 Need regular tracking - Trimming A by 50 20 70 20, reference by 20 20 20 20 Tracking image shifted -0.006, -0.005 um; position 0.160, 0.265 Drift reached 0.3 A/sec after 6.6 sec, going on 4 frames were saved to X:\Luke_20191213\tomo-g2_00046_-43.0.tif Frame alignment results: distance raw = 1.3 smoothed = 1.3 Residual mean = 0.02 max max = 0.03 Saved Z = 0 at 0.00 min Tilt = -42.98 Mean = 13 IA = 2.15um dose = 1.98 e/A2 X/Y/Z = 0.160 0.266 -0.807 Need regular tracking - 4 frames were saved to X:\Luke_20191213\tomo-g2_00047_-45.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.8 Residual mean = 0.04 max max = 0.07 Trimming A by 100 22 80 20, reference by 100 21 85 20 Record image shifted 0.002, -0.009 um Saved Z = 1 at 1.01 min Tilt = -44.99 Mean = 12 IA = 2.15um dose = 1.98 e/A2 X/Y/Z = -0.002 0.009 -0.807 Trimming A by 105 24 85 20, reference by 100 22 80 20 Need regular tracking - Tracking image shifted 0.000, -0.003 um; position -0.003, 0.012 Ending Navigator acquisition after current item 4 frames were saved to X:\Luke_20191213\tomo-g2_00048_-47.0.tif Frame alignment results: distance raw = 0.8 smoothed = 0.6 Residual mean = 0.05 max max = 0.09 2019/12/13 16:05 X:\Luke_20191213\s1_3part.st 0 2 11520 8184 377 -43.0 -45.0 2.00 REG 1.0 1x1 GIF-K3 33000 1 0.13 LD 20.0 0.0 Total dose in electrons/square Angstrom: Recording: 4.0 Tracking: 3.9 Focusing: 0.0 Tasks: 1.97 Previews: 0.2 Ending Navigator acquisition after current item 4 frames were saved to X:\Luke_20191213\tomo-g2_00049_-43.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.2 Residual mean = 0.09 max max = 0.14 4 frames were saved to X:\Luke_20191213\tomo-g2_00050_-49.0.tif Frame alignment results: distance raw = 0.7 smoothed = 0.6 Residual mean = 0.04 max max = 0.05