Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.41 0.76 (2nd move error 0.06 -0.25) Trimming A by 40 20 20 40, reference by 43 20 20 20 Disparity in stage position after aligning to map item itself: 0.17 0.92 (align shift 0.06 -0.03) Finished aligning to item in 2 rounds Dewars are NOT busy filling Refrigerant level in dewar 1 is 49.853 Refrigerant level in dewar 2 is 71.163 Opened new file X:\Luke_20200318\YGA_s1.mrc STARTING TILT SERIES WITH AUTOMATIC TERMINATION AFTER ERRORS. Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.000 um; position -0.094, 0.240 Drift reached 0.6 A/sec after 6.7 sec, going on Measured defocus = -5.40 microns changed by 0.40 to target drift = 0.04 nm/sec Measured defocus = -5.16 microns changed by 0.16 to target drift = 0.03 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00000_9.0.tif Frame alignment results: distance raw = 1.1 smoothed = 1.1 Residual mean = 0.03 max max = 0.05 Saved Z = 0 at 0.00 min Tilt = 8.99 Mean = 76 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.094 0.240 -1.337 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.030 um; position -0.098, 0.270 Drift reached 2.8 A/sec after 6.7 sec, going on Measured defocus = -5.08 microns changed by 0.08 to target drift = 0.11 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00001_7.0.tif Frame alignment results: distance raw = 1.2 smoothed = 0.5 Residual mean = 0.01 max max = 0.01 Trimming A by 25 30, reference by 25 30 Record image shifted -0.000, 0.004 um Tracking reference shifted by 0.2,-7.0 pixels, -0.000,0.004 um Saved Z = 1 at 0.47 min Tilt = 7.00 Mean = 77 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.098 0.269 -1.254 Predicted X = -0.102 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.298 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = -1.171 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.004, 0.000 um; position -0.099, 0.297 Drift reached 1.2 A/sec after 6.8 sec, going on Measured defocus = -4.96 microns changed by -0.04 to target drift = 0.06 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00002_5.0.tif Frame alignment results: distance raw = 0.7 smoothed = 0.5 Residual mean = 0.02 max max = 0.04 Trimming A by 25 25, reference by 25 30 Record image shifted -0.000, 0.009 um Tracking reference shifted by 0.2,-17.8 pixels, -0.000,0.009 um Saved Z = 2 at 0.94 min Tilt = 5.00 Mean = 79 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.098 0.289 -1.207 Errors in X/Y Prediction 0.004 -0.009 um, 0.4 0.8 % Error in Z Prediction -0.036 Need regular tracking - Need autofocus Tracking image shifted -0.003, -0.021 um; position -0.091, 0.261 Drift reached 2.4 A/sec after 6.8 sec, going on Measured defocus = -5.02 microns changed by 0.02 to target drift = 0.11 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00003_11.0.tif Frame alignment results: distance raw = 0.7 smoothed = 0.7 Residual mean = 0.02 max max = 0.04 Trimming A by 25 25, reference by 25 30 Record image shifted -0.000, -0.003 um Tracking reference shifted by 1.0,5.4 pixels, -0.000,-0.003 um Saved Z = 3 at 1.47 min Tilt = 11.00 Mean = 74 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.090 0.262 -1.312 Need regular tracking - Need autofocus Tracking image shifted 0.006, 0.045 um; position -0.096, 0.217 Drift reached 1.8 A/sec after 6.8 sec, going on Measured defocus = -5.04 microns changed by 0.04 to target drift = 0.09 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00004_13.0.tif Frame alignment results: distance raw = 1.3 smoothed = 0.8 Residual mean = 0.01 max max = 0.02 Trimming A by 20 20, reference by 25 25 Record image shifted -0.000, -0.015 um Tracking reference shifted by 1.6,29.2 pixels, -0.000,-0.015 um Saved Z = 4 at 1.98 min Tilt = 13.00 Mean = 73 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.095 0.234 -1.272 Predicted X = -0.101 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.315 StdErr = 0.006 nFit = 3 nDrop = 0 Predicted Z = -1.136 StdErr = 0.027 nFit = 3 nDrop = 0 Drift reached 0.6 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00005_3.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.4 Residual mean = 0.03 max max = 0.05 Trimming A by 30 35, reference by 25 25 Record image shifted -0.002, 0.085 um Saved Z = 5 at 2.33 min Tilt = 3.01 Mean = 77 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.099 0.229 -1.136 Errors in X/Y Prediction 0.002 -0.085 um, 0.2 8.1 % Predicted X = -0.099 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.223 StdErr = 0.059 nFit = 3 nDrop = 0 Predicted Z = -1.072 StdErr = 0.035 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, -0.046 um; position -0.099, 0.269 Drift reached 0.3 A/sec after 6.7 sec, going on Measured defocus = -4.86 microns changed by -0.14 to target drift = 0.03 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00006_1.0.tif Frame alignment results: distance raw = 0.8 smoothed = 0.7 Residual mean = 0.02 max max = 0.03 Trimming A by 20 25, reference by 30 35 Record image shifted -0.000, 0.016 um Tracking reference shifted by 0.0,-31.6 pixels, -0.000,0.016 um Saved Z = 6 at 2.81 min Tilt = 1.01 Mean = 82 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.099 0.252 -1.207 Errors in X/Y Prediction 0.000 0.029 um, 0.0 2.8 % Error in Z Prediction -0.135 Predicted X = -0.100 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.205 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = -1.232 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.015, -0.102 um; position -0.085, 0.308 Drift reached 0.6 A/sec after 6.7 sec, going on Measured defocus = -4.90 microns changed by -0.10 to target drift = 0.03 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00007_15.0.tif Frame alignment results: distance raw = 0.8 smoothed = 0.7 Residual mean = 0.03 max max = 0.05 Trimming A by 25 25, reference by 20 20 Record image shifted -0.001, -0.009 um Tracking reference shifted by 2.1,16.6 pixels, -0.001,-0.009 um Saved Z = 7 at 3.34 min Tilt = 15.00 Mean = 71 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.084 0.317 -1.328 Errors in X/Y Prediction 0.016 0.111 um, 1.6 10.6 % Error in Z Prediction -0.096 Predicted X = -0.084 StdErr = 0.012 nFit = 3 nDrop = 0 Predicted Y = 0.325 StdErr = 0.083 nFit = 3 nDrop = 0 Predicted Z = -1.320 StdErr = 0.072 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, 0.012 um; position -0.081, 0.314 Drift reached 0.3 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00008_17.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.7 Residual mean = 0.03 max max = 0.05 Trimming A by 20 20, reference by 25 25 Record image shifted 0.001, -0.006 um Tracking reference shifted by -2.0,12.3 pixels, 0.001,-0.006 um Saved Z = 8 at 3.74 min Tilt = 17.00 Mean = 69 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.082 0.320 -1.320 Errors in X/Y Prediction 0.002 -0.005 um, 0.2 0.5 % Predicted X = -0.099 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.220 StdErr = 0.061 nFit = 3 nDrop = 0 Predicted Z = -1.187 StdErr = 0.042 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, 0.014 um; position -0.095, 0.206 Drift reached 2.6 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00009_-1.0.tif Frame alignment results: distance raw = 0.7 smoothed = 0.1 Residual mean = 0.03 max max = 0.04 Trimming A by 20 20 20 20, reference by 25 20 20 20 Record image shifted 0.000, 0.003 um Tracking reference shifted by -0.5,-6.1 pixels, 0.000,0.003 um Saved Z = 9 at 4.15 min Tilt = -0.99 Mean = 85 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.095 0.205 -1.187 Errors in X/Y Prediction 0.004 -0.015 um, 0.4 1.4 % Predicted X = -0.094 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.205 StdErr = 0.052 nFit = 3 nDrop = 0 Predicted Z = -1.173 StdErr = 0.051 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, 0.002 um; position -0.096, 0.203 Drift reached 0.3 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00010_-3.0.tif Frame alignment results: distance raw = 0.7 smoothed = 0.4 Residual mean = 0.03 max max = 0.04 Record image shifted 0.000, 0.010 um Tracking reference shifted by -0.8,-19.2 pixels, 0.000,0.010 um Saved Z = 10 at 4.52 min Tilt = -2.98 Mean = 86 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.096 0.193 -1.173 Errors in X/Y Prediction -0.002 -0.012 um, 0.2 1.1 % Predicted X = -0.074 StdErr = 0.007 nFit = 3 nDrop = 0 Predicted Y = 0.376 StdErr = 0.059 nFit = 3 nDrop = 0 Predicted Z = -1.328 StdErr = 0.095 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.008, 0.036 um; position -0.082, 0.341 Drift reached 2.6 A/sec after 12.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00011_19.0.tif Frame alignment results: distance raw = 1.1 smoothed = 1.0 Residual mean = 0.04 max max = 0.05 Record image shifted 0.001, 0.009 um Tracking reference shifted by -2.0,-17.7 pixels, 0.001,0.009 um Saved Z = 11 at 5.06 min Tilt = 19.00 Mean = 67 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.082 0.337 -1.328 Errors in X/Y Prediction -0.008 -0.039 um, 0.8 3.8 % Predicted X = -0.081 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.345 StdErr = 0.010 nFit = 3 nDrop = 0 Predicted Z = -1.336 StdErr = 0.120 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.000 um; position -0.081, 0.344 Drift reached 0.7 A/sec after 6.8 sec, going on Measured defocus = -4.88 microns changed by -0.12 to target drift = 0.01 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00012_21.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.9 Residual mean = 0.03 max max = 0.05 Record image shifted 0.000, 0.019 um Tracking reference shifted by -1.3,-37.1 pixels, 0.000,0.019 um Saved Z = 12 at 5.57 min Tilt = 21.00 Mean = 65 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.081 0.325 -1.456 Errors in X/Y Prediction -0.000 -0.020 um, 0.0 1.9 % Error in Z Prediction -0.120 Predicted X = -0.096 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = 0.137 StdErr = 0.070 nFit = 4 nDrop = 0 Predicted Z = -1.159 StdErr = 0.061 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.003, 0.008 um; position -0.093, 0.130 Drift reached 2.6 A/sec after 6.9 sec, going on Measured defocus = -4.79 microns changed by -0.21 to target drift = 0.13 nm/sec Measured defocus = -4.94 microns changed by -0.06 to target drift = 0.11 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00013_-5.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.4 Residual mean = 0.02 max max = 0.04 Record image shifted 0.000, 0.001 um Tracking reference shifted by -0.8,-2.4 pixels, 0.000,0.001 um Saved Z = 13 at 6.19 min Tilt = -4.99 Mean = 88 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.094 0.130 -1.427 Errors in X/Y Prediction 0.003 -0.007 um, 0.3 0.7 % Error in Z Prediction -0.268 Predicted X = -0.093 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = 0.057 StdErr = 0.040 nFit = 5 nDrop = 0 Predicted Z = -1.450 StdErr = 0.111 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.003, -0.072 um; position -0.097, 0.129 Drift reached 0.5 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00014_-7.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.6 Residual mean = 0.03 max max = 0.05 Record image shifted -0.001, 0.012 um Tracking reference shifted by 1.2,-23.1 pixels, -0.001,0.012 um Saved Z = 14 at 6.56 min Tilt = -6.99 Mean = 88 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.096 0.117 -1.450 Errors in X/Y Prediction -0.003 0.060 um, 0.3 5.8 % Predicted X = -0.081 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.332 StdErr = 0.022 nFit = 3 nDrop = 0 Predicted Z = -1.503 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, -0.076 um; position -0.080, 0.408 Drift reached 2.6 A/sec after 18.9 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00015_23.0.tif Frame alignment results: distance raw = 1.1 smoothed = 0.6 Residual mean = 0.04 max max = 0.06 Record image shifted -0.001, 0.005 um Tracking reference shifted by 2.5,-10.7 pixels, -0.001,0.005 um Saved Z = 15 at 7.21 min Tilt = 23.00 Mean = 64 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.077 0.417 -1.503 Errors in X/Y Prediction 0.003 0.084 um, 0.3 8.0 % Predicted X = -0.076 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.513 StdErr = 0.088 nFit = 4 nDrop = 0 Predicted Z = -1.548 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.116 um; position -0.074, 0.397 Drift reached 0.3 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00016_25.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.7 Residual mean = 0.03 max max = 0.06 Record image shifted 0.002, 0.003 um Tracking reference shifted by -4.5,-6.0 pixels, 0.002,0.003 um Saved Z = 16 at 7.62 min Tilt = 25.00 Mean = 61 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.077 0.393 -1.548 Errors in X/Y Prediction -0.001 -0.119 um, 0.1 11.4 % Predicted X = -0.096 StdErr = 0.006 nFit = 4 nDrop = 0 Predicted Y = 0.054 StdErr = 0.041 nFit = 6 nDrop = 0 Predicted Z = -1.497 StdErr = 0.125 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.005, -0.020 um; position -0.092, 0.074 Drift reached 2.8 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00017_-9.0.tif Frame alignment results: distance raw = 1.1 smoothed = 0.5 Residual mean = 0.04 max max = 0.06 Record image shifted 0.001, 0.004 um Tracking reference shifted by -2.6,-8.1 pixels, 0.001,0.004 um Saved Z = 17 at 8.05 min Tilt = -8.99 Mean = 90 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.093 0.072 -1.497 Errors in X/Y Prediction 0.003 0.018 um, 0.3 1.7 % Predicted X = -0.094 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.040 StdErr = 0.028 nFit = 6 nDrop = 0 Predicted Z = -1.543 StdErr = 0.141 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.006, -0.072 um; position -0.100, 0.112 Drift reached 2.0 A/sec after 6.9 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00018_-11.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.0 Residual mean = 0.03 max max = 0.05 Record image shifted -0.001, 0.008 um Tracking reference shifted by 1.6,-15.2 pixels, -0.001,0.008 um Saved Z = 18 at 8.42 min Tilt = -10.99 Mean = 91 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.099 0.105 -1.543 Errors in X/Y Prediction -0.005 0.066 um, 0.5 6.3 % Predicted X = -0.075 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = 0.438 StdErr = 0.084 nFit = 5 nDrop = 0 Predicted Z = -1.593 StdErr = 0.017 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.008, 0.018 um; position -0.083, 0.420 Stopping the wait for drift: drift is still 3.3 A/sec after 19 sec; going on * * * * * * * * * * * * * * * * * * TILT SERIES TERMINATING WITH THIS MESSAGE: Waiting for drift has failed * * * * * * * * * * * * * * * * * * 2020/03/18 15:51 X:\Luke_20200318\YGA_s1.mrc 0 19 5760 4092 895 9.0 -11.0 2.00 REG 8.4 1x1 GIF-K3 33000 2 0.26 LD-DS 20.0 0.0 Total dose in electrons/square Angstrom: Recording: 38.6 Tracking: 27.4 Focusing: 37.0 Tasks: 0.00 Previews: 0.0 Measured defocus = -5.07 microns changed by 0.07 to target drift = 0.18 nm/sec Need regular tracking - Need autofocus Measured defocus = -5.31 microns changed by 0.31 to target drift = 0.09 nm/sec Measured defocus = -5.11 microns changed by 0.11 to target drift = 0.06 nm/sec Need regular tracking - Need autofocus Tracking image shifted 0.000, 0.003 um; position -0.007, -0.208 Drift reached 0.3 A/sec after 6.7 sec, going on Measured defocus = -5.02 microns changed by 0.02 to target drift = 0.04 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00019_27.0.tif Frame alignment results: distance raw = 2.0 smoothed = 1.9 Residual mean = 0.05 max max = 0.09 Saved Z = 0 at -0.00 min Tilt = 26.98 Mean = 57 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.007 -0.209 -1.080 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.091 um; position -0.007, -0.118 Drift reached 0.8 A/sec after 7.0 sec, going on Measured defocus = -4.97 microns changed by -0.03 to target drift = 0.07 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00020_29.0.tif Frame alignment results: distance raw = 1.4 smoothed = 0.9 Residual mean = 0.06 max max = 0.09 Record image shifted -0.000, 0.006 um Tracking reference shifted by 0.4,-11.8 pixels, -0.000,0.006 um Saved Z = 1 at 0.47 min Tilt = 28.98 Mean = 55 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.007 -0.125 -1.114 Predicted X = -0.006 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = -0.041 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = -1.149 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.004, 0.146 um; position -0.002, -0.186 Drift reached 1.1 A/sec after 6.8 sec, going on Measured defocus = -5.04 microns changed by 0.04 to target drift = 0.07 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00021_31.0.tif Frame alignment results: distance raw = 1.5 smoothed = 1.4 Residual mean = 0.04 max max = 0.06 Record image shifted 0.002, 0.007 um Tracking reference shifted by -3.9,-13.8 pixels, 0.002,0.007 um Saved Z = 2 at 0.94 min Tilt = 30.99 Mean = 52 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.004 -0.194 -1.110 Errors in X/Y Prediction 0.002 -0.154 um, 0.1 14.6 % Error in Z Prediction 0.039 Predicted X = -0.003 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.162 StdErr = 0.114 nFit = 3 nDrop = 0 Predicted Z = -1.132 StdErr = 0.029 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, -0.023 um; position -0.005, -0.139 Drift reached 0.9 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00022_33.0.tif Frame alignment results: distance raw = 1.3 smoothed = 1.1 Residual mean = 0.06 max max = 0.08 Record image shifted -0.001, 0.001 um Tracking reference shifted by 2.4,-2.5 pixels, -0.001,0.001 um Saved Z = 3 at 1.31 min Tilt = 32.98 Mean = 50 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.003 -0.141 -1.132 Errors in X/Y Prediction -0.000 0.021 um, 0.0 2.0 % Predicted X = -0.002 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.170 StdErr = 0.092 nFit = 3 nDrop = 0 Predicted Z = -1.147 StdErr = 0.038 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, -0.098 um; position -0.004, -0.071 Drift reached 0.8 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00023_35.0.tif Frame alignment results: distance raw = 1.7 smoothed = 1.6 Residual mean = 0.04 max max = 0.06 Record image shifted -0.000, 0.004 um Tracking reference shifted by 0.4,-7.8 pixels, -0.000,0.004 um Saved Z = 4 at 1.68 min Tilt = 34.98 Mean = 47 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.004 -0.076 -1.147 Errors in X/Y Prediction -0.002 0.093 um, 0.2 8.9 % Predicted X = -0.003 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.019 StdErr = 0.009 nFit = 3 nDrop = 0 Predicted Z = -1.163 StdErr = 0.049 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.093 um; position -0.003, -0.112 Drift reached 1.0 A/sec after 6.8 sec, going on Measured defocus = -5.10 microns changed by 0.10 to target drift = 0.06 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00024_37.0.tif Frame alignment results: distance raw = 1.9 smoothed = 1.7 Residual mean = 0.05 max max = 0.08 Record image shifted 0.002, 0.006 um Tracking reference shifted by -4.1,-11.8 pixels, 0.002,0.006 um Saved Z = 5 at 2.15 min Tilt = 36.98 Mean = 44 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.005 -0.119 -1.061 Errors in X/Y Prediction -0.002 -0.100 um, 0.2 9.5 % Error in Z Prediction 0.101 Predicted X = -0.006 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.089 StdErr = 0.080 nFit = 3 nDrop = 0 Predicted Z = -1.049 StdErr = 0.012 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, 0.035 um; position -0.008, -0.125 Drift reached 0.9 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00025_39.0.tif Frame alignment results: distance raw = 1.6 smoothed = 1.5 Residual mean = 0.05 max max = 0.07 Record image shifted -0.000, 0.002 um Tracking reference shifted by 0.1,-3.9 pixels, -0.000,0.002 um Saved Z = 6 at 2.51 min Tilt = 38.98 Mean = 41 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.008 -0.128 -1.049 Errors in X/Y Prediction -0.002 -0.038 um, 0.2 3.6 % Predicted X = -0.011 StdErr = 0.000 nFit = 4 nDrop = 0 Predicted Y = -0.209 StdErr = 0.093 nFit = 4 nDrop = 0 Predicted Z = -1.035 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.122 um; position -0.013, -0.087 Drift reached 1.2 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00026_41.0.tif Frame alignment results: distance raw = 2.0 smoothed = 1.8 Residual mean = 0.03 max max = 0.05 Record image shifted -0.001, 0.001 um Tracking reference shifted by 0.9,-2.5 pixels, -0.001,0.001 um Saved Z = 7 at 2.87 min Tilt = 40.98 Mean = 39 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.012 -0.089 -1.035 Errors in X/Y Prediction -0.001 0.120 um, 0.1 11.4 % Predicted X = -0.015 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.105 StdErr = 0.086 nFit = 5 nDrop = 0 Predicted Z = -1.021 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, 0.006 um; position -0.011, -0.110 Drift reached 0.9 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00027_43.0.tif Frame alignment results: distance raw = 1.8 smoothed = 1.5 Residual mean = 0.09 max max = 0.17 Record image shifted 0.001, 0.004 um Tracking reference shifted by -1.5,-7.4 pixels, 0.001,0.004 um Saved Z = 8 at 3.24 min Tilt = 42.98 Mean = 35 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.012 -0.115 -1.021 Errors in X/Y Prediction 0.003 -0.010 um, 0.3 1.0 % Predicted X = -0.012 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = -0.124 StdErr = 0.061 nFit = 6 nDrop = 0 Predicted Z = -1.007 StdErr = 0.018 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.017 um; position -0.016, -0.107 Drift reached 0.8 A/sec after 6.8 sec, going on Measured defocus = -5.05 microns changed by 0.05 to target drift = 0.05 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00028_45.0.tif Frame alignment results: distance raw = 2.4 smoothed = 2.1 Residual mean = 0.08 max max = 0.12 Record image shifted 0.000, 0.003 um Tracking reference shifted by -0.6,-5.1 pixels, 0.000,0.003 um Saved Z = 9 at 3.72 min Tilt = 44.98 Mean = 32 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.017 -0.110 -0.962 Errors in X/Y Prediction -0.005 0.014 um, 0.4 1.4 % Error in Z Prediction 0.045 Predicted X = -0.018 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = -0.121 StdErr = 0.042 nFit = 5 nDrop = 0 Predicted Z = -0.944 StdErr = 0.018 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.013 um; position -0.020, -0.108 Drift reached 0.6 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00029_47.0.tif Frame alignment results: distance raw = 2.1 smoothed = 2.0 Residual mean = 0.08 max max = 0.12 Record image shifted -0.000, 0.002 um Tracking reference shifted by 0.3,-3.5 pixels, -0.000,0.002 um Saved Z = 10 at 4.09 min Tilt = 46.98 Mean = 30 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.020 -0.110 -0.944 Errors in X/Y Prediction -0.001 0.011 um, 0.1 1.0 % Predicted X = -0.024 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = -0.120 StdErr = 0.030 nFit = 6 nDrop = 0 Predicted Z = -0.923 StdErr = 0.019 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, -0.045 um; position -0.022, -0.075 Drift reached 0.4 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00030_49.0.tif Frame alignment results: distance raw = 1.6 smoothed = 1.5 Residual mean = 0.07 max max = 0.09 Record image shifted -0.000, 0.002 um Tracking reference shifted by 0.8,-3.6 pixels, -0.000,0.002 um Saved Z = 11 at 4.45 min Tilt = 48.98 Mean = 27 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.022 -0.077 -0.923 Errors in X/Y Prediction 0.002 0.043 um, 0.1 4.1 % Predicted X = -0.025 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = -0.036 StdErr = 0.015 nFit = 5 nDrop = 0 Predicted Z = -0.901 StdErr = 0.021 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.003, 0.007 um; position -0.022, -0.043 Drift reached 0.6 A/sec after 6.8 sec, going on Measured defocus = -4.93 microns changed by -0.07 to target drift = 0.05 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00031_51.0.tif Frame alignment results: distance raw = 2.1 smoothed = 2.0 Residual mean = 0.06 max max = 0.09 Record image shifted 0.000, 0.004 um Tracking reference shifted by -0.8,-6.9 pixels, 0.000,0.004 um Saved Z = 12 at 4.93 min Tilt = 50.98 Mean = 24 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.022 -0.047 -0.969 Errors in X/Y Prediction 0.003 -0.011 um, 0.2 1.0 % Error in Z Prediction -0.067 Predicted X = -0.024 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.001 StdErr = 0.015 nFit = 5 nDrop = 0 Predicted Z = -0.937 StdErr = 0.055 nFit = 3 nDrop = 0 Drift reached 0.3 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00032_53.0.tif Frame alignment results: distance raw = 2.0 smoothed = 1.9 Residual mean = 0.09 max max = 0.15 Record image shifted -0.002, 0.034 um Saved Z = 13 at 5.26 min Tilt = 52.98 Mean = 21 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.022 -0.033 -0.937 Errors in X/Y Prediction 0.002 -0.034 um, 0.2 3.3 % Predicted X = -0.023 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.002 StdErr = 0.027 nFit = 5 nDrop = 0 Predicted Z = -0.923 StdErr = 0.060 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.003, 0.012 um; position -0.020, -0.009 Drift reached 0.4 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00033_55.0.tif Frame alignment results: distance raw = 1.3 smoothed = 1.2 Residual mean = 0.03 max max = 0.05 Record image shifted -0.001, 0.003 um Tracking reference shifted by 1.2,-6.5 pixels, -0.001,0.003 um Saved Z = 14 at 5.63 min Tilt = 54.98 Mean = 19 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.019 -0.013 -0.923 Errors in X/Y Prediction 0.003 -0.015 um, 0.3 1.5 % Predicted X = -0.015 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = -0.006 StdErr = 0.009 nFit = 5 nDrop = 1 Predicted Z = -0.909 StdErr = 0.066 nFit = 3 nDrop = 0 Drift reached 0.1 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00034_57.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.8 Residual mean = 0.09 max max = 0.15 Record image shifted 0.003, -0.044 um Saved Z = 15 at 5.95 min Tilt = 56.97 Mean = 17 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.018 0.038 -0.909 Errors in X/Y Prediction -0.003 0.044 um, 0.3 4.2 % Predicted X = -0.015 StdErr = 0.004 nFit = 4 nDrop = 0 Predicted Y = 0.063 StdErr = 0.023 nFit = 6 nDrop = 0 Predicted Z = -0.896 StdErr = 0.071 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.002, -0.017 um; position -0.017, 0.080 Drift reached 0.2 A/sec after 6.7 sec, going on Measured defocus = -0.02 microns changed by -4.98 to target drift = 0.00 nm/sec Measured defocus = -0.01 microns changed by -4.99 to target drift = 0.00 nm/sec Measured defocus = -0.00 microns changed by -5.00 to target drift = 0.00 nm/sec Measured defocus = -0.02 microns changed by -4.98 to target drift = 0.00 nm/sec Measured defocus = -0.01 microns changed by -4.99 to target drift = 0.00 nm/sec Measured defocus = 0.08 microns changed by -5.08 to target drift = 0.01 nm/sec Measured defocus = 0.00 microns changed by -5.00 to target drift = 0.00 nm/sec Measured defocus = -0.07 microns drift = 0.00 nm/sec WARNING: Autofocus aborted after 8 iterations and focus returned to starting value (-5.90) because too may iterations have been run. Check focus calibration and/or scope alignment if images and specimen look OK Measured defocus = 0.00 microns changed by -5.00 to target drift = 0.00 nm/sec Measured defocus = -0.01 microns changed by -4.99 to target drift = 0.01 nm/sec Measured defocus = 0.01 microns changed by -5.01 to target drift = 0.00 nm/sec Measured defocus = 0.08 microns changed by -5.08 to target drift = 0.00 nm/sec Measured defocus = 0.04 microns changed by -5.04 to target drift = 0.01 nm/sec Measured defocus = 0.01 microns changed by -5.01 to target drift = 0.00 nm/sec Measured defocus = -0.05 microns changed by -4.95 to target drift = 0.01 nm/sec Measured defocus = -0.05 microns changed by -4.95 to target drift = 0.01 nm/sec Measured defocus = -0.02 microns changed by -4.98 to target drift = 0.00 nm/sec Measured defocus = 0.03 microns changed by -5.03 to target drift = 0.01 nm/sec Measured defocus = 0.02 microns changed by -5.02 to target drift = 0.00 nm/sec Measured defocus = 0.03 microns changed by -5.03 to target drift = 0.00 nm/sec Measured defocus = -0.01 microns changed by -4.99 to target drift = 0.00 nm/sec Measured defocus = -0.02 microns changed by -4.98 to target drift = 0.00 nm/sec 2020/03/18 16:12 X:\Luke_20200318\YGA_s1_part2.mrc 0 16 5760 4092 754 27.0 57.0 2.00 REG 6.0 1x1 GIF-K3 33000 2 0.26 LD 20.0 0.0 Total dose in electrons/square Angstrom: Recording: 32.5 Tracking: 20.6 Focusing: 32.9 Tasks: 2.74 Previews: 0.0 Measured defocus = -60.18 microns changed by 16.00 drift = 0.12 nm/sec Measured defocus = -49.23 microns changed by 16.00 drift = 0.26 nm/sec Measured defocus = -37.28 microns changed by 16.00 drift = 0.17 nm/sec Measured defocus = -25.17 microns changed by 16.00 drift = 0.15 nm/sec Measured defocus = -12.83 microns changed by 7.83 to target drift = 0.15 nm/sec Measured defocus = -6.97 microns changed by 1.97 to target drift = 0.11 nm/sec Measured defocus = -5.49 microns changed by 0.49 to target drift = 0.09 nm/sec Measured defocus = -5.15 microns changed by 0.15 to target drift = 0.07 nm/sec Need regular tracking - Need autofocus Tracking image shifted -0.000, -0.000 um; position 0.004, 0.004 Drift reached 0.4 A/sec after 6.7 sec, going on Measured defocus = -5.02 microns changed by 0.02 to target drift = 0.02 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00035_-13.0.tif Frame alignment results: distance raw = 0.8 smoothed = 0.7 Residual mean = 0.07 max max = 0.10 Record image shifted -0.001, -0.002 um Tracking reference shifted by 1.6,4.0 pixels, -0.001,-0.002 um Saved Z = 0 at -0.00 min Tilt = -12.98 Mean = 101 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = 0.005 0.006 3.496 Need regular tracking - Need autofocus Tracking image shifted -0.024, -0.223 um; position 0.030, 0.229 Drift reached 1.6 A/sec after 6.7 sec, going on Measured defocus = -5.06 microns changed by 0.06 to target drift = 0.07 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00036_-15.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.3 Residual mean = 0.05 max max = 0.08 Record image shifted -0.001, 0.002 um Tracking reference shifted by 2.6,-3.1 pixels, -0.001,0.002 um Saved Z = 1 at 0.46 min Tilt = -14.99 Mean = 101 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = 0.031 0.229 3.561 Predicted X = 0.056 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Y = 0.451 StdErr = 0.000 nFit = 2 nDrop = 0 Predicted Z = 3.625 StdErr = 0.000 nFit = 2 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.025, 0.221 um; position 0.031, 0.230 Drift reached 1.4 A/sec after 6.8 sec, going on Measured defocus = -4.95 microns changed by -0.05 to target drift = 0.06 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00037_-17.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.4 Residual mean = 0.04 max max = 0.07 Record image shifted 0.005, 0.014 um Tracking reference shifted by -10.7,-27.1 pixels, 0.005,0.014 um Saved Z = 2 at 0.94 min Tilt = -16.99 Mean = 101 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = 0.026 0.217 3.577 Errors in X/Y Prediction -0.030 -0.234 um, 2.9 22.3 % Error in Z Prediction -0.048 Predicted X = 0.041 StdErr = 0.022 nFit = 3 nDrop = 0 Predicted Y = 0.361 StdErr = 0.175 nFit = 3 nDrop = 0 Predicted Z = 3.625 StdErr = 0.036 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.021, 0.132 um; position 0.021, 0.229 Drift reached 1.3 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00038_-19.0.tif Frame alignment results: distance raw = 0.8 smoothed = 0.2 Residual mean = 0.04 max max = 0.06 Record image shifted -0.001, 0.007 um Tracking reference shifted by 1.8,-14.5 pixels, -0.001,0.007 um Saved Z = 3 at 1.30 min Tilt = -18.98 Mean = 102 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = 0.021 0.223 3.625 Errors in X/Y Prediction -0.020 -0.138 um, 1.9 13.2 % Predicted X = 0.017 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.216 StdErr = 0.014 nFit = 3 nDrop = 0 Predicted Z = 3.665 StdErr = 0.047 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.040 um; position 0.016, 0.257 Drift reached 1.7 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00039_-21.0.tif Frame alignment results: distance raw = 0.7 smoothed = 0.1 Residual mean = 0.06 max max = 0.08 Record image shifted -0.002, 0.006 um Tracking reference shifted by 2.9,-11.9 pixels, -0.002,0.006 um Saved Z = 4 at 1.66 min Tilt = -20.98 Mean = 102 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = 0.017 0.252 3.665 Errors in X/Y Prediction 0.000 0.035 um, 0.0 3.4 % Predicted X = 0.013 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.266 StdErr = 0.017 nFit = 3 nDrop = 0 Predicted Z = 3.705 StdErr = 0.060 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.000, 0.023 um; position 0.013, 0.243 Drift reached 1.1 A/sec after 6.7 sec, going on Measured defocus = -4.97 microns changed by -0.03 to target drift = 0.07 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00040_-23.0.tif Frame alignment results: distance raw = 1.1 smoothed = 0.3 Residual mean = 0.04 max max = 0.07 Record image shifted 0.001, 0.008 um Tracking reference shifted by -2.4,-15.2 pixels, 0.001,0.008 um Saved Z = 5 at 2.13 min Tilt = -22.98 Mean = 101 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = 0.012 0.236 3.673 Errors in X/Y Prediction -0.001 -0.030 um, 0.1 2.9 % Error in Z Prediction -0.033 Predicted X = 0.008 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.250 StdErr = 0.034 nFit = 3 nDrop = 0 Predicted Z = 3.700 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, 0.032 um; position 0.008, 0.218 Drift reached 1.3 A/sec after 6.9 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00041_-25.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.1 Residual mean = 0.06 max max = 0.09 Record image shifted -0.001, 0.009 um Tracking reference shifted by 0.7,-17.0 pixels, -0.001,0.009 um Saved Z = 6 at 2.49 min Tilt = -24.98 Mean = 101 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = 0.008 0.210 3.700 Errors in X/Y Prediction 0.001 -0.039 um, 0.1 3.8 % Predicted X = 0.005 StdErr = 0.002 nFit = 4 nDrop = 0 Predicted Y = 0.149 StdErr = 0.024 nFit = 4 nDrop = 0 Predicted Z = 3.729 StdErr = 0.017 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.051 um; position 0.003, 0.199 Drift reached 1.6 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00042_-27.0.tif Frame alignment results: distance raw = 0.7 smoothed = 0.1 Residual mean = 0.04 max max = 0.06 Record image shifted -0.001, 0.007 um Tracking reference shifted by 1.0,-13.2 pixels, -0.001,0.007 um Saved Z = 7 at 2.86 min Tilt = -26.98 Mean = 101 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = 0.004 0.194 3.729 Errors in X/Y Prediction -0.001 0.045 um, 0.1 4.3 % Predicted X = -0.001 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.142 StdErr = 0.029 nFit = 5 nDrop = 0 Predicted Z = 3.758 StdErr = 0.020 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.061 um; position 0.000, 0.203 Drift reached 1.7 A/sec after 6.7 sec, going on Measured defocus = -5.03 microns changed by 0.03 to target drift = 0.07 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00043_-29.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.4 Residual mean = 0.03 max max = 0.05 Record image shifted 0.000, 0.007 um Tracking reference shifted by -0.9,-13.3 pixels, 0.000,0.007 um Saved Z = 8 at 3.33 min Tilt = -28.98 Mean = 100 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.000 0.197 3.791 Errors in X/Y Prediction 0.000 0.055 um, 0.0 5.2 % Error in Z Prediction 0.034 Predicted X = -0.005 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.197 StdErr = 0.016 nFit = 5 nDrop = 1 Predicted Z = 3.823 StdErr = 0.014 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, 0.034 um; position -0.005, 0.163 Drift reached 1.6 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00044_-31.0.tif Frame alignment results: distance raw = 1.0 smoothed = 0.4 Residual mean = 0.04 max max = 0.06 Record image shifted 0.000, 0.010 um Tracking reference shifted by -1.4,-18.7 pixels, 0.000,0.010 um Saved Z = 9 at 3.69 min Tilt = -30.98 Mean = 99 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.005 0.154 3.823 Errors in X/Y Prediction -0.000 -0.042 um, 0.0 4.0 % Predicted X = -0.010 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.138 StdErr = 0.033 nFit = 5 nDrop = 0 Predicted Z = 3.859 StdErr = 0.015 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.023 um; position -0.009, 0.114 Drift reached 1.2 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00045_-33.0.tif Frame alignment results: distance raw = 1.1 smoothed = 0.3 Residual mean = 0.05 max max = 0.09 Record image shifted 0.000, 0.008 um Tracking reference shifted by -1.3,-15.6 pixels, 0.000,0.008 um Saved Z = 10 at 4.05 min Tilt = -32.99 Mean = 98 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.010 0.107 3.859 Errors in X/Y Prediction -0.000 -0.031 um, 0.0 2.9 % Predicted X = -0.015 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.045 StdErr = 0.026 nFit = 5 nDrop = 0 Predicted Z = 3.895 StdErr = 0.017 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted -0.001, -0.086 um; position -0.014, 0.131 Drift reached 1.7 A/sec after 6.8 sec, going on Measured defocus = -4.97 microns changed by -0.03 to target drift = 0.09 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00046_-35.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.4 Residual mean = 0.03 max max = 0.05 Record image shifted -0.001, 0.006 um Tracking reference shifted by 1.5,-11.9 pixels, -0.001,0.006 um Saved Z = 11 at 4.53 min Tilt = -34.99 Mean = 97 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.013 0.126 3.860 Errors in X/Y Prediction 0.001 0.081 um, 0.1 7.7 % Error in Z Prediction -0.035 Predicted X = -0.017 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.102 StdErr = 0.060 nFit = 5 nDrop = 0 Predicted Z = 3.900 StdErr = 0.030 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.002, 0.004 um; position -0.019, 0.098 Drift reached 1.3 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00047_-37.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.4 Residual mean = 0.09 max max = 0.14 Record image shifted 0.000, 0.009 um Tracking reference shifted by -1.2,-17.8 pixels, 0.000,0.009 um Saved Z = 12 at 4.89 min Tilt = -36.99 Mean = 95 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.020 0.089 3.900 Errors in X/Y Prediction -0.002 -0.013 um, 0.2 1.2 % Predicted X = -0.024 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.101 StdErr = 0.047 nFit = 5 nDrop = 0 Predicted Z = 3.931 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.012 um; position -0.023, 0.089 Drift reached 1.1 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00048_-39.0.tif Frame alignment results: distance raw = 0.7 smoothed = 0.5 Residual mean = 0.04 max max = 0.05 Record image shifted 0.000, 0.008 um Tracking reference shifted by -1.0,-16.1 pixels, 0.000,0.008 um Saved Z = 13 at 5.25 min Tilt = -38.99 Mean = 94 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.024 0.082 3.931 Errors in X/Y Prediction 0.001 -0.019 um, 0.0 1.8 % Predicted X = -0.029 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.074 StdErr = 0.046 nFit = 5 nDrop = 0 Predicted Z = 3.962 StdErr = 0.037 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, -0.025 um; position -0.027, 0.100 Drift reached 1.3 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00049_-41.0.tif Frame alignment results: distance raw = 1.0 smoothed = 0.6 Residual mean = 0.06 max max = 0.12 Record image shifted 0.000, 0.008 um Tracking reference shifted by -0.5,-15.4 pixels, 0.000,0.008 um Saved Z = 14 at 5.61 min Tilt = -40.99 Mean = 91 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.027 0.093 3.962 Errors in X/Y Prediction 0.002 0.018 um, 0.2 1.8 % Predicted X = -0.029 StdErr = 0.001 nFit = 4 nDrop = 0 Predicted Y = 0.083 StdErr = 0.044 nFit = 5 nDrop = 0 Predicted Z = 3.993 StdErr = 0.041 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, -0.029 um; position -0.031, 0.113 Drift reached 1.2 A/sec after 6.8 sec, going on Measured defocus = -5.02 microns changed by 0.02 to target drift = 0.07 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00050_-43.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.6 Residual mean = 0.10 max max = 0.16 Record image shifted 0.000, 0.008 um Tracking reference shifted by -1.1,-15.5 pixels, 0.000,0.008 um Saved Z = 15 at 6.08 min Tilt = -42.99 Mean = 89 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.031 0.105 4.013 Errors in X/Y Prediction -0.002 0.022 um, 0.2 2.1 % Error in Z Prediction 0.020 Predicted X = -0.035 StdErr = 0.000 nFit = 3 nDrop = 0 Predicted Y = 0.147 StdErr = 0.014 nFit = 5 nDrop = 0 Predicted Z = 4.037 StdErr = 0.032 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.004, 0.035 um; position -0.031, 0.111 Drift reached 1.0 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00051_-45.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.4 Residual mean = 0.07 max max = 0.13 Record image shifted -0.000, 0.010 um Tracking reference shifted by -0.4,-18.8 pixels, -0.000,0.010 um Saved Z = 16 at 6.45 min Tilt = -44.99 Mean = 87 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.031 0.102 4.037 Errors in X/Y Prediction 0.004 -0.044 um, 0.4 4.2 % Predicted X = -0.030 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Y = 0.115 StdErr = 0.018 nFit = 5 nDrop = 1 Predicted Z = 4.069 StdErr = 0.035 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.001, 0.007 um; position -0.029, 0.107 Drift reached 1.0 A/sec after 6.9 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00052_-47.0.tif Frame alignment results: distance raw = 0.8 smoothed = 0.4 Residual mean = 0.07 max max = 0.10 Record image shifted -0.000, 0.008 um Tracking reference shifted by -0.0,-15.1 pixels, -0.000,0.008 um Saved Z = 17 at 6.81 min Tilt = -46.99 Mean = 84 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.029 0.100 4.069 Errors in X/Y Prediction 0.001 -0.014 um, 0.1 1.4 % Predicted X = -0.028 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = 0.090 StdErr = 0.007 nFit = 5 nDrop = 0 Predicted Z = 4.101 StdErr = 0.038 nFit = 3 nDrop = 0 Need autofocus Drift reached 0.5 A/sec after 6.8 sec, going on Measured defocus = -5.03 microns changed by 0.03 to target drift = 0.09 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00053_-49.0.tif Frame alignment results: distance raw = 0.8 smoothed = 0.5 Residual mean = 0.09 max max = 0.17 Record image shifted 0.000, -0.031 um Saved Z = 18 at 7.23 min Tilt = -48.98 Mean = 81 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.028 0.121 4.133 Errors in X/Y Prediction -0.000 0.031 um, 0.0 2.9 % Error in Z Prediction 0.031 Predicted X = -0.026 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Y = 0.120 StdErr = 0.016 nFit = 6 nDrop = 0 Predicted Z = 4.170 StdErr = 0.003 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.002, -0.021 um; position -0.024, 0.141 Drift reached 1.1 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00054_-51.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.3 Residual mean = 0.06 max max = 0.08 Record image shifted 0.000, 0.017 um Tracking reference shifted by -1.1,-33.6 pixels, 0.000,0.017 um Saved Z = 19 at 7.59 min Tilt = -50.98 Mean = 78 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.025 0.124 4.170 Errors in X/Y Prediction 0.001 0.004 um, 0.1 0.4 % Predicted X = -0.022 StdErr = 0.003 nFit = 4 nDrop = 0 Predicted Y = 0.145 StdErr = 0.015 nFit = 5 nDrop = 0 Predicted Z = 4.209 StdErr = 0.004 nFit = 3 nDrop = 0 Drift reached 0.5 A/sec after 6.9 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00055_-53.0.tif Frame alignment results: distance raw = 0.7 smoothed = 0.3 Residual mean = 0.05 max max = 0.09 Record image shifted 0.005, 0.076 um Saved Z = 20 at 7.91 min Tilt = -52.98 Mean = 74 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.027 0.070 4.209 Errors in X/Y Prediction -0.005 -0.076 um, 0.5 7.2 % Predicted X = -0.026 StdErr = 0.004 nFit = 3 nDrop = 0 Predicted Y = 0.030 StdErr = 0.043 nFit = 5 nDrop = 0 Predicted Z = 4.248 StdErr = 0.004 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.004, -0.051 um; position -0.030, 0.081 Drift reached 1.4 A/sec after 6.8 sec, going on Measured defocus = -5.06 microns changed by 0.06 to target drift = 0.08 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00056_-55.0.tif Frame alignment results: distance raw = 0.8 smoothed = 0.6 Residual mean = 0.11 max max = 0.15 Record image shifted 0.001, 0.015 um Tracking reference shifted by -2.3,-30.0 pixels, 0.001,0.015 um Saved Z = 21 at 8.38 min Tilt = -54.99 Mean = 71 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.031 0.067 4.309 Errors in X/Y Prediction -0.005 0.037 um, 0.5 3.5 % Error in Z Prediction 0.060 Predicted X = -0.034 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.009 StdErr = 0.047 nFit = 5 nDrop = 0 Predicted Z = 4.349 StdErr = 0.034 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.004, -0.039 um; position -0.039, 0.048 Drift reached 1.2 A/sec after 6.7 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00057_-57.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.4 Residual mean = 0.10 max max = 0.15 Record image shifted 0.000, 0.011 um Tracking reference shifted by -0.6,-20.6 pixels, 0.000,0.011 um Saved Z = 22 at 8.76 min Tilt = -56.99 Mean = 67 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.039 0.038 4.349 Errors in X/Y Prediction -0.005 0.030 um, 0.5 2.8 % Predicted X = -0.044 StdErr = 0.003 nFit = 3 nDrop = 0 Predicted Y = 0.011 StdErr = 0.041 nFit = 5 nDrop = 0 Predicted Z = 4.398 StdErr = 0.038 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted 0.001, -0.010 um; position -0.045, 0.021 Drift reached 1.0 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00058_-59.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.2 Residual mean = 0.06 max max = 0.09 Record image shifted 0.000, 0.010 um Tracking reference shifted by -1.2,-18.9 pixels, 0.000,0.010 um Saved Z = 23 at 9.12 min Tilt = -58.99 Mean = 63 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.046 0.012 4.398 Errors in X/Y Prediction -0.001 0.001 um, 0.1 0.1 % Predicted X = -0.053 StdErr = 0.001 nFit = 3 nDrop = 0 Predicted Y = 0.001 StdErr = 0.034 nFit = 5 nDrop = 0 Predicted Z = 4.447 StdErr = 0.042 nFit = 3 nDrop = 0 Need regular tracking - Need autofocus Tracking image shifted 0.001, 0.030 um; position -0.054, -0.029 Drift reached 1.0 A/sec after 6.7 sec, going on Measured defocus = -5.02 microns changed by 0.02 to target drift = 0.06 nm/sec 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00059_-61.0.tif Frame alignment results: distance raw = 0.6 smoothed = 0.3 Residual mean = 0.11 max max = 0.17 Record image shifted 0.001, 0.010 um Tracking reference shifted by -2.2,-19.8 pixels, 0.001,0.010 um Saved Z = 24 at 9.59 min Tilt = -60.99 Mean = 60 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.055 -0.039 4.463 Errors in X/Y Prediction -0.002 -0.040 um, 0.2 3.8 % Error in Z Prediction 0.016 Predicted X = -0.063 StdErr = 0.002 nFit = 3 nDrop = 0 Predicted Y = -0.099 StdErr = 0.011 nFit = 5 nDrop = 0 Predicted Z = 4.522 StdErr = 0.013 nFit = 3 nDrop = 0 Need regular tracking - Tracking image shifted -0.000, -0.054 um; position -0.063, -0.045 Drift reached 1.2 A/sec after 6.8 sec, going on 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00060_-63.0.tif Frame alignment results: distance raw = 0.5 smoothed = 0.2 Residual mean = 0.08 max max = 0.12 Record image shifted -0.001, 0.007 um Tracking reference shifted by 2.5,-14.1 pixels, -0.001,0.007 um Saved Z = 25 at 9.96 min Tilt = -62.99 Mean = 56 IA = 2.15um dose = 2.03 e/A2 X/Y/Z = -0.062 -0.052 4.522 2020/03/18 16:27 X:\Luke_20200318\YGA_s1_part3.mrc 0 26 5760 4092 1225 -13.0 -63.0 2.00 REG 10.0 1x1 GIF-K3 33000 2 0.26 LD 20.0 0.0 Total dose in electrons/square Angstrom: Recording: 52.8 Tracking: 34.3 Focusing: 60.7 Tasks: 0.00 Previews: 0.2 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00061_-65.0.tif Frame alignment results: distance raw = 0.9 smoothed = 0.7 Residual mean = 0.11 max max = 0.17 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00062_-67.0.tif Frame alignment results: distance raw = 0.3 smoothed = 0.1 Residual mean = 0.14 max max = 0.22 4 frames were saved to X:\Luke_20200318\YGA-g1_s1_00063_-68.9.tif Frame alignment results: distance raw = 0.6 smoothed = 0.4 Residual mean = 0.10 max max = 0.13