Talos Arctica 9950423 SerialEM Version 3.8.8 64-bit, built Apr 3 2021 03:39:50 Started 5/14/2021 10:07:47 Special option is set to disable automatic trimming of dark borders in Autoalign for some cases Read settings from: C:\ProgramData\SerialEM\SerialEMsettings.txt Read properties/calibrations from: C:\ProgramData\SerialEM\ Read scripts from C:\ProgramData\SerialEM\SerialEMsettings-scripts.txt Rough eucentricity: changing Z by -25.36 to -25.36, continuing... Rough eucentricity: changing Z by 7.32 to -18.04, finished. Updated state # 5 0.70 A, 50 um C2 with current imaging state Tilt-induced shift is 131.25, -314.02 unbinned pixels, unfiltered CCC is -0.0040 The average drift between shots was -0.4, -1.6 unbinned pixels, 0.06 nm/sec Measured defocus = 6.15 microns changed by -6.65 to target Tilt-induced shift is -16.49, 32.71 unbinned pixels, unfiltered CCC is 0.0309 The average drift between shots was -2.0, -2.7 unbinned pixels, 0.12 nm/sec Measured defocus = -0.66 microns changed by 0.16 to target Tilt-induced shift is -14.28, 25.37 unbinned pixels, unfiltered CCC is 0.0346 The average drift between shots was -1.1, -1.9 unbinned pixels, 0.08 nm/sec Measured defocus = -0.53 microns changed by 0.03 to target Refining eucentricity: Fit to 8 positions gives tilt axis Z displacement of 2.11 microns and lateral displacement of -2.55 microns Tilt-induced shift is -147.50, 339.33 unbinned pixels, unfiltered CCC is -0.0054 The average drift between shots was -3.6, 0.5 unbinned pixels, 0.13 nm/sec Measured defocus = -6.75 microns changed by 6.25 to target Tilt-induced shift is -28.57, 55.26 unbinned pixels, unfiltered CCC is 0.0407 The average drift between shots was -1.5, -0.5 unbinned pixels, 0.05 nm/sec Measured defocus = -1.13 microns changed by 0.63 to target Tilt-induced shift is -15.43, 26.54 unbinned pixels, unfiltered CCC is 0.0602 The average drift between shots was -2.5, -1.2 unbinned pixels, 0.10 nm/sec Measured defocus = -0.55 microns changed by 0.05 to target Tilt-induced shift is -14.08, 24.04 unbinned pixels, unfiltered CCC is 0.1026 The average drift between shots was -2.3, -0.8 unbinned pixels, 0.06 nm/sec Measured defocus = -0.50 microns Ctffind: defocus: -0.307, astig: 0.309 um, angle: -57.7, score 0.5332, fit to 4.4 A Objective stigmator was adjusted by -0.0033 -0.0088 Ctffind: defocus: -0.314, astig: 0.009 um, angle: -69.4, score 0.5116, fit to 4.4 A Objective stigmator was adjusted by 0.0000 -0.0003 Changing defocus to -1.00 to get an acceptable number of CTF rings Ctffind: defocus: -0.667, astig: 0.013 um, angle: 47.2, score 0.7599, fit to 3.8 A Ctffind: defocus: -0.727, astig: 0.175 um, angle: 74.7, score 0.7464, fit to 3.8 A Ctffind: defocus: -0.339, astig: 0.383 um, angle: 9.4, score 0.5768, fit to 4.4 A Ctffind: defocus: -0.375, astig: 0.337 um, angle: -48.3, score 0.5811, fit to 4.2 A Ctffind: defocus: -0.687, astig: 0.206 um, angle: 68.4, score 0.7464, fit to 3.9 A Beam tilt was adjusted by -3.62 2.94 milliradians Iterating because beam tilt is above threshold Ctffind: defocus: -0.778, astig: 0.144 um, angle: 71.7, score 0.7530, fit to 3.8 A Ctffind: defocus: -0.642, astig: 0.197 um, angle: 44.7, score 0.7338, fit to 3.8 A Ctffind: defocus: -0.649, astig: 0.162 um, angle: 48.4, score 0.7377, fit to 3.8 A Ctffind: defocus: -0.651, astig: 0.179 um, angle: -88.5, score 0.7317, fit to 3.8 A Ctffind: defocus: -0.638, astig: 0.212 um, angle: -85.6, score 0.7406, fit to 3.9 A Beam tilt was adjusted by 0.01 -0.08 milliradians Ctffind: defocus: -0.421, astig: 0.123 um, angle: 73.4, score 0.6690, fit to 4.2 A Objective stigmator was adjusted by 0.0037 -0.0008 Changing defocus to -1.00 to get an acceptable number of CTF rings Ctffind: defocus: -0.776, astig: 0.015 um, angle: 47.8, score 0.7517, fit to 3.8 A Ctffind: defocus: -0.642, astig: 0.149 um, angle: 23.7, score 0.7292, fit to 4.0 A Ctffind: defocus: -0.649, astig: 0.132 um, angle: 23.1, score 0.7343, fit to 4.0 A Ctffind: defocus: -0.647, astig: 0.111 um, angle: -67.7, score 0.7372, fit to 4.0 A Ctffind: defocus: -0.643, astig: 0.119 um, angle: -67.6, score 0.7429, fit to 4.0 A Beam tilt was adjusted by 0.05 -0.03 milliradians Changing defocus to -1.00 to get an acceptable number of CTF rings Ctffind: defocus: -0.777, astig: 0.017 um, angle: 38.9, score 0.7506, fit to 4.1 A Ctffind: defocus: -0.645, astig: 0.143 um, angle: 23.1, score 0.7322, fit to 4.0 A Ctffind: defocus: -0.646, astig: 0.139 um, angle: 22.5, score 0.7394, fit to 4.0 A Ctffind: defocus: -0.646, astig: 0.111 um, angle: -67.1, score 0.7355, fit to 4.0 A Ctffind: defocus: -0.646, astig: 0.107 um, angle: -67.7, score 0.7438, fit to 4.0 A Beam tilt was adjusted by 0.00 -0.01 milliradians Ctffind: defocus: -0.430, astig: 0.005 um, angle: 7.1, score 0.6648, fit to 4.2 A Objective stigmator was adjusted by -0.0001 0.0001 Frame alignment results: distance raw = 13.0 smoothed = 7.6 Weighted resid mean = 0.02 max max = 0.04 Mean unweighted max = 0.11 Frame alignment results: distance raw = 12.1 smoothed = 6.5 Weighted resid mean = 0.02 max max = 0.05 Mean unweighted max = 0.10 Frame alignment results: distance raw = 10.2 smoothed = 5.1 Weighted resid mean = 0.03 max max = 0.07 Mean unweighted max = 0.15 Measuring at image shift -3.0 0.0 um (-2.8 0.0 IS units) Tilt-induced shift is -4.17, 6.97 pixels, unfiltered CCC is 0.1711 The average drift between shots was -2.3, -1.3 pixels, 0.04 nm/sec Measured defocus = -0.15 microns Changing defocus to -1.00 to get an acceptable number of CTF rings Ctffind: defocus: -0.783, astig: 0.024 um, angle: -63.0, score 0.8877, fit to 3.2 A Ctffind: defocus: -0.614, astig: 0.124 um, angle: 17.7, score 0.8857, fit to 2.8 A Ctffind: defocus: -0.686, astig: 0.063 um, angle: 23.0, score 0.8491, fit to 3.5 A Ctffind: defocus: -0.660, astig: 0.151 um, angle: -71.5, score 0.8577, fit to 3.4 A Ctffind: defocus: -0.638, astig: 0.161 um, angle: -56.9, score 0.8947, fit to 3.0 A Beam tilt needs to be adjusted by 0.68 -0.22 milliradians Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -0.423, astig: 0.042 um, angle: -59.5, score 0.9097, fit to 3.0 A Objective stigmator needs to be adjusted by -0.0004 -0.0012 Measuring at image shift 3.0 0.0 um (2.8 0.0 IS units) Changing defocus to -1.00 to get an acceptable number of CTF rings Ctffind: defocus: -1.018, astig: 0.082 um, angle: 33.4, score 0.4130, fit to 4.8 A Ctffind: defocus: -0.913, astig: 0.178 um, angle: 26.7, score 0.7471, fit to 4.6 A Ctffind: defocus: -0.843, astig: 0.253 um, angle: 23.4, score 0.8416, fit to 4.1 A Ctffind: defocus: -0.867, astig: 0.049 um, angle: -53.1, score 0.8018, fit to 4.3 A Ctffind: defocus: -0.888, astig: 0.053 um, angle: 76.0, score 0.8337, fit to 4.3 A Beam tilt needs to be adjusted by -0.62 0.17 milliradians Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -0.650, astig: 0.083 um, angle: 26.8, score 0.8515, fit to 4.4 A Objective stigmator needs to be adjusted by 0.0004 0.0025 Measuring at image shift 0.0 -3.0 um (0.0 -2.8 IS units) Changing defocus to -1.00 to get an acceptable number of CTF rings Ctffind: defocus: -0.905, astig: 0.057 um, angle: 27.8, score 0.7278, fit to 3.8 A Ctffind: defocus: -0.787, astig: 0.165 um, angle: 16.9, score 0.8568, fit to 3.5 A Ctffind: defocus: -0.756, astig: 0.203 um, angle: 29.3, score 0.8914, fit to 3.5 A Ctffind: defocus: -0.809, astig: 0.031 um, angle: -61.6, score 0.8586, fit to 3.5 A Ctffind: defocus: -0.729, astig: 0.104 um, angle: -73.2, score 0.8771, fit to 3.7 A Beam tilt needs to be adjusted by -0.28 -0.73 milliradians Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -0.544, astig: 0.051 um, angle: 24.2, score 0.8936, fit to 3.6 A Objective stigmator needs to be adjusted by 0.0001 0.0015 Measuring at image shift 0.0 3.0 um (0.0 2.8 IS units) Changing defocus to -1.00 to get an acceptable number of CTF rings Ctffind: defocus: -0.820, astig: 0.005 um, angle: 74.2, score 0.7161, fit to 2.8 A Ctffind: defocus: -0.668, astig: 0.132 um, angle: 29.7, score 0.6583, fit to 5.1 A Ctffind: defocus: -0.703, astig: 0.109 um, angle: 12.8, score 0.7618, fit to 4.6 A Ctffind: defocus: -0.642, astig: 0.182 um, angle: -70.5, score 0.7194, fit to 4.2 A Ctffind: defocus: -0.721, astig: 0.088 um, angle: -65.2, score 0.7183, fit to 4.0 A Beam tilt needs to be adjusted by 0.35 0.69 milliradians Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -0.459, astig: 0.015 um, angle: -58.9, score 0.7136, fit to 5.4 A Objective stigmator needs to be adjusted by -0.0001 -0.0004 IS to beam tilt matrix: -0.232090 0.109807 0.068969 0.250665 IS to astigmatism matrix: 0.000142 -0.000045 0.000663 -0.000348 Rough eucentricity: changing Z by 95.38 to 95.41, continuing... Rough eucentricity: changing Z by 14.42 to 109.83, finished. Maximum piece coordinates are above 65535, too large to store in the image file header. Piece coordinates will be saved only in a Metadata Autodoc (.mdoc) file The overview binning needed to be increased to get memory for the overview image You will probably be able to see it at full resolution by making it a map and loading the map with conversion to bytes The overview binning needed to be increased to get memory for the overview image You will probably be able to see it at full resolution by making it a map and loading the map with conversion to bytes The original displacements in the overlap zones have mean 119.21 maximum 619.69 pixels. After shifting pieces into register, the displacements have mean 72.88 maximum 228.16 pixels. The original displacements in the overlap zones have mean 118.21 maximum 626.36 pixels. After shifting pieces into register, the displacements have mean 72.88 maximum 228.16 pixels. The original displacements in the overlap zones have mean 118.21 maximum 626.36 pixels. After shifting pieces into register, the displacements have mean 72.88 maximum 228.16 pixels. Rough eucentricity: changing Z by 129.38 to 129.41, continuing... Rough eucentricity: changing Z by 102.92 to 232.34, continuing... Rough eucentricity: changing Z by 21.16 to 253.50, continuing... Rough eucentricity: changing Z by -1.28 to 252.22, finished. The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0006.tif The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. Rough eucentricity: changing Z by -22.80 to 229.41, continuing... Rough eucentricity: changing Z by -1.52 to 227.89, finished. Setting to standard focus (-0.005959) since this is low mag (540x) Tilt-induced shift is 20.35, -48.42 unbinned pixels, unfiltered CCC is 0.0185 The average drift between shots was -18.4, 17.0 unbinned pixels, 0.70 nm/sec Measured defocus = 0.95 microns changed by -3.95 to target Tilt-induced shift is -66.25, 154.89 unbinned pixels, unfiltered CCC is 0.0171 The average drift between shots was -7.4, -4.7 unbinned pixels, 0.25 nm/sec Measured defocus = -3.06 microns changed by 0.06 to target 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0007.tif The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. Rough eucentricity: changing Z by 66.88 to 319.09, continuing... Rough eucentricity: changing Z by -108.44 to 210.66, finished. Opened new file X:\users\am2837\20210514\cgAM262_20210514\squares.mrc Map acquired at item # 7 with label 7 saved at Z = 0 Backlash adjustment to stage coordinates is -0.01, -0.04 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -15.72 to 236.49, finished. Map acquired at item # 8 with label 8 saved at Z = 1 Backlash adjustment to stage coordinates is -0.02, -0.10 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -12.04 to 240.17, finished. Map acquired at item # 9 with label 9 saved at Z = 2 Backlash adjustment to stage coordinates is -0.01, -0.10 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -9.75 to 242.46, finished. Map acquired at item # 10 with label 10 saved at Z = 3 Backlash adjustment to stage coordinates is -0.01, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -6.29 to 245.92, finished. Map acquired at item # 11 with label 11 saved at Z = 4 Backlash adjustment to stage coordinates is -0.02, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -2.87 to 249.34, finished. Map acquired at item # 12 with label 12 saved at Z = 5 Backlash adjustment to stage coordinates is -0.01, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -2.10 to 250.11, finished. Map acquired at item # 13 with label 13 saved at Z = 6 Backlash adjustment to stage coordinates is -0.01, -0.06 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -5.91 to 246.30, finished. Map acquired at item # 14 with label 14 saved at Z = 7 Backlash adjustment to stage coordinates is -0.02, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -13.71 to 238.50, finished. Map acquired at item # 15 with label 15 saved at Z = 8 Backlash adjustment to stage coordinates is -0.02, -0.06 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -75.51 to 176.71, continuing... Rough eucentricity: changing Z by 1.05 to 177.75, finished. Map acquired at item # 16 with label 16 saved at Z = 9 Backlash adjustment to stage coordinates is -0.01, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -82.87 to 169.34, continuing... Rough eucentricity: changing Z by 0.34 to 169.69, finished. Map acquired at item # 17 with label 17 saved at Z = 10 Backlash adjustment to stage coordinates is -0.00, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -50.35 to 201.86, continuing... Rough eucentricity: changing Z by -0.65 to 201.22, finished. Map acquired at item # 18 with label 18 saved at Z = 11 Backlash adjustment to stage coordinates is -0.03, -0.09 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -40.91 to 211.30, finished. Map acquired at item # 19 with label 19 saved at Z = 12 Backlash adjustment to stage coordinates is -0.02, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -114.54 to 137.68, continuing... Rough eucentricity: changing Z by 0.95 to 138.62, finished. Map acquired at item # 20 with label 20 saved at Z = 13 Backlash adjustment to stage coordinates is -0.02, -0.06 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -96.10 to 156.11, continuing... Rough eucentricity: changing Z by -27.77 to 128.34, continuing... Rough eucentricity: changing Z by -4.77 to 123.57, finished. Map acquired at item # 21 with label 21 saved at Z = 14 Backlash adjustment to stage coordinates is -0.02, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -92.00 to 160.22, continuing... Rough eucentricity: changing Z by -2.60 to 157.62, finished. Map acquired at item # 22 with label 22 saved at Z = 15 Backlash adjustment to stage coordinates is -0.02, -0.09 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -57.17 to 195.04, continuing... Rough eucentricity: changing Z by -0.32 to 194.72, finished. Map acquired at item # 23 with label 23 saved at Z = 16 Backlash adjustment to stage coordinates is -0.02, -0.09 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -30.20 to 222.01, continuing... Rough eucentricity: changing Z by -1.33 to 220.69, finished. Map acquired at item # 24 with label 24 saved at Z = 17 Backlash adjustment to stage coordinates is -0.03, -0.09 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -11.51 to 240.70, finished. Map acquired at item # 25 with label 25 saved at Z = 18 Backlash adjustment to stage coordinates is -0.03, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -12.31 to 239.90, finished. Map acquired at item # 26 with label 26 saved at Z = 19 Backlash adjustment to stage coordinates is -0.04, -0.10 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -1.79 to 250.42, finished. Map acquired at item # 27 with label 27 saved at Z = 20 Backlash adjustment to stage coordinates is -0.03, -0.06 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -18.61 to 233.60, continuing... Rough eucentricity: changing Z by -1.86 to 231.74, finished. Map acquired at item # 28 with label 28 saved at Z = 21 Backlash adjustment to stage coordinates is -0.02, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -21.49 to 230.72, continuing... Rough eucentricity: changing Z by -1.61 to 229.11, finished. Map acquired at item # 29 with label 29 saved at Z = 22 Backlash adjustment to stage coordinates is -0.01, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -15.93 to 236.28, continuing... Rough eucentricity: changing Z by -1.64 to 234.64, finished. Map acquired at item # 30 with label 30 saved at Z = 23 Backlash adjustment to stage coordinates is -0.04, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -46.83 to 205.37, continuing... Rough eucentricity: changing Z by -1.65 to 203.72, finished. Map acquired at item # 31 with label 31 saved at Z = 24 Backlash adjustment to stage coordinates is -0.02, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -84.56 to 167.65, continuing... Rough eucentricity: changing Z by 2.44 to 170.09, finished. Map acquired at item # 32 with label 32 saved at Z = 25 Backlash adjustment to stage coordinates is -0.03, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -76.65 to 175.56, continuing... Rough eucentricity: changing Z by 0.93 to 176.48, finished. Map acquired at item # 33 with label 33 saved at Z = 26 Backlash adjustment to stage coordinates is -0.03, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -118.70 to 133.51, finished. Map acquired at item # 34 with label 34 saved at Z = 27 Backlash adjustment to stage coordinates is -0.06, -0.09 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by 0.53 to 252.74, finished. Map acquired at item # 35 with label 35 saved at Z = 28 Backlash adjustment to stage coordinates is -0.03, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -3.04 to 249.17, finished. Map acquired at item # 36 with label 36 saved at Z = 29 Backlash adjustment to stage coordinates is -0.03, -0.09 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by 2.36 to 254.57, finished. Map acquired at item # 37 with label 37 saved at Z = 30 Backlash adjustment to stage coordinates is -0.04, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -11.02 to 241.19, finished. Map acquired at item # 38 with label 38 saved at Z = 31 Backlash adjustment to stage coordinates is -0.02, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -7.41 to 244.80, finished. Map acquired at item # 39 with label 39 saved at Z = 32 Backlash adjustment to stage coordinates is -0.03, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -19.52 to 232.69, continuing... Rough eucentricity: changing Z by -1.27 to 231.42, finished. Map acquired at item # 40 with label 40 saved at Z = 33 Backlash adjustment to stage coordinates is -0.03, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -18.77 to 233.44, continuing... Rough eucentricity: changing Z by -1.87 to 231.57, finished. Map acquired at item # 41 with label 41 saved at Z = 34 Backlash adjustment to stage coordinates is -0.02, -0.08 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -18.86 to 233.36, continuing... Rough eucentricity: changing Z by -1.69 to 231.66, finished. Map acquired at item # 42 with label 42 saved at Z = 35 Backlash adjustment to stage coordinates is -0.02, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -20.30 to 231.91, continuing... Rough eucentricity: changing Z by -1.78 to 230.13, finished. Map acquired at item # 43 with label 43 saved at Z = 36 Backlash adjustment to stage coordinates is -0.02, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -96.33 to 155.88, continuing... Rough eucentricity: changing Z by -39.50 to 116.38, continuing... Rough eucentricity: changing Z by 4.35 to 120.73, finished. Map acquired at item # 44 with label 44 saved at Z = 37 Backlash adjustment to stage coordinates is -0.03, -0.07 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -92.95 to 159.27, continuing... Rough eucentricity: changing Z by -1.40 to 157.86, finished. Map acquired at item # 45 with label 45 saved at Z = 38 Backlash adjustment to stage coordinates is -0.03, -0.09 The real map is now in Buffer C - be careful to mark points only on that! Rough eucentricity: changing Z by -58.16 to 194.05, continuing... Rough eucentricity: changing Z by -1.65 to 192.40, finished. Map acquired at item # 46 with label 46 saved at Z = 39 Backlash adjustment to stage coordinates is -0.03, -0.09 The real map is now in Buffer C - be careful to mark points only on that! The original displacements in the overlap zones have mean 85.72 maximum 85.72 pixels. After shifting pieces into register, the displacements have mean 0.00 maximum 0.00 pixels. The original displacements in the overlap zones have mean 85.72 maximum 85.72 pixels. After shifting pieces into register, the displacements have mean 0.00 maximum 0.00 pixels. Full View shift offset is set to -0.013, 0.074 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Low Dose Record parameters will be restored when you finish defining the pattern Low Dose Record parameters were restored to original values Low Dose Record parameters will be restored when you finish defining the pattern Low Dose Record parameters were restored to original values Low Dose Record parameters will be restored when you finish defining the pattern Low Dose Record parameters were restored to original values Low Dose Record parameters will be restored when you finish defining the pattern Low Dose Record parameters were restored to original values Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0008.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0009.tif Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0010.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0011.tif Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0012.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0013.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0014.tif Tilt-induced shift is -1172.34, -1934.48 unbinned pixels, unfiltered CCC is -0.0017 The average drift between shots was -6.8, -0.6 unbinned pixels, 0.19 nm/sec Measured defocus = 22.28 microns changed by -16.00 Using peak at -206.2,24.1, rejecting 1.46 times higher peak at 0.0,-0.2 Tilt-induced shift is -206.05, -24.30 unbinned pixels, unfiltered CCC is 0.0075 The average drift between shots was -206.3, -23.9 unbinned pixels, 5.61 nm/sec Measured defocus = -1.08 microns changed by -1.92 to target Tilt-induced shift is 137.24, -220.66 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was 416.9, 221.1 unbinned pixels, 13.15 nm/sec Measured defocus = 4.65 microns WARNING: Autofocus aborted after 3 iterations and focus returned to starting value (-3.12) because of inconsistent behavior. Check focus calibration and/or scope alignment if images and specimen look OK Tilt-induced shift is 0.02, 0.00 unbinned pixels, unfiltered CCC is 0.1197 The average drift between shots was 0.1, -0.1 unbinned pixels, 0.00 nm/sec Measured defocus = 0.00 microns changed by -3.00 to target Tilt-induced shift is -0.02, 0.12 unbinned pixels, unfiltered CCC is 0.1185 The average drift between shots was 0.1, 0.1 unbinned pixels, 0.00 nm/sec Measured defocus = -0.00 microns WARNING: Autofocus aborted after 2 iterations and focus returned to starting value (-3.12) because of inconsistent behavior. Check focus calibration and/or scope alignment if images and specimen look OK Tilt-induced shift is -0.01, -0.00 pixels, unfiltered CCC is 0.2376 The average drift between shots was 0.0, -0.0 pixels, 0.00 nm/sec Measured defocus = 0.00 microns Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -1.077, astig: 0.361 um, angle: 0.0, score 0.0460, fit to 7.7 A Tilt-induced shift is -0.00, -0.00 pixels, unfiltered CCC is 0.0237 The average drift between shots was 0.0, -0.0 pixels, 0.00 nm/sec Measured defocus = 0.00 microns Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -1.055, astig: 0.201 um, angle: 0.7, score 0.0624, fit to 4.4 A Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -0.999, astig: 0.012 um, angle: 12.2, score 0.0346, fit to 3.8 A Tilt-induced shift is 0.00, 0.00 pixels, unfiltered CCC is 0.2257 The average drift between shots was 0.0, -0.0 pixels, 0.00 nm/sec Measured defocus = 0.00 microns Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -0.740, astig: 0.278 um, angle: -0.4, score 0.0388, fit to 4.8 A 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0016.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0017.tif Tilt-induced shift is -0.00, -0.00 pixels, unfiltered CCC is 0.0683 The average drift between shots was -0.0, 0.0 pixels, 0.00 nm/sec Measured defocus = 0.00 microns Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -0.846, astig: 0.292 um, angle: 3.2, score 0.0481, fit to 324.4 A Tilt-induced shift is 0.00, 0.00 pixels, unfiltered CCC is 0.0492 The average drift between shots was -0.0, -0.0 pixels, 0.00 nm/sec Measured defocus = 0.00 microns Changing defocus to -0.50 to get an acceptable number of CTF rings Ctffind: defocus: -1.006, astig: 0.025 um, angle: 49.5, score 0.0502, fit to 324.4 A Tilt-induced shift is -1176.05, -1924.81 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -3.7, -1.0 unbinned pixels, 0.09 nm/sec Measured defocus = 22.10 microns changed by -16.00 Tilt-induced shift is -0.04, 0.07 unbinned pixels, unfiltered CCC is 0.0184 The average drift between shots was -0.0, -0.1 unbinned pixels, 0.00 nm/sec Measured defocus = -0.00 microns changed by -3.00 to target Using peak at -11.9,-36.0, rejecting 1.62 times higher peak at 0.1,0.1 Tilt-induced shift is 11.94, -35.95 unbinned pixels, unfiltered CCC is 0.0066 The average drift between shots was -11.9, 36.0 unbinned pixels, 0.86 nm/sec Measured defocus = 0.69 microns WARNING: Autofocus aborted after 3 iterations and focus returned to starting value (-3.12) because of inconsistent behavior. Check focus calibration and/or scope alignment if images and specimen look OK EDIT MODE REMINDER: Left-click to select Ctrl-Left-Click to add or remove from selected points Left-double-click to delete point if already selected Shift-Left-double-click to remove one position from multiple Record array Middle-click to add a point Right-click to move current point Backspace to delete current point Tilt-induced shift is -84.15, 197.99 unbinned pixels, unfiltered CCC is 0.0046 The average drift between shots was -5.9, 1.5 unbinned pixels, 0.14 nm/sec Measured defocus = -3.91 microns changed by 0.91 to target Tilt-induced shift is -67.41, 157.45 unbinned pixels, unfiltered CCC is 0.0031 The average drift between shots was -3.0, 0.8 unbinned pixels, 0.07 nm/sec Measured defocus = -3.11 microns changed by 0.11 to target Tilt-induced shift is -65.46, 152.73 unbinned pixels, unfiltered CCC is 0.0029 The average drift between shots was -2.8, 1.6 unbinned pixels, 0.07 nm/sec Measured defocus = -3.02 microns changed by 0.02 to target Low Dose Record parameters will be restored when you finish defining the pattern Low Dose Record parameters were restored to original values Low Dose Record parameters will be restored when you finish defining the pattern Low Dose Record parameters were restored to original values Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0018.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0019.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0020.tif Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0021.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0022.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0023.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0024.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0025.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0026.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0027.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0028.tif 50 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0029.tif Full View shift offset is set to -0.354, 0.159 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to -0.221, 0.376 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to -0.538, -0.095 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Min = 0.0, max = 1379.0, mean = 474.95, SD = 121.82 Pixel size = 0.253 nm 13.945 electrons (484.62 counts) per unbinned pixel per second Min = 162.0, max = 3164.0, mean = 1584.26, SD = 254.13 Pixel size = 5.91 nm 11.993 electrons (489.22 counts) per unbinned pixel per second Full View shift offset is set to 0.136, 0.265 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to -0.190, 0.006 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to 0.006, 0.181 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to -0.132, 0.095 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to -0.069, 0.144 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again The original displacements in the overlap zones have mean 105.78 maximum 603.72 pixels. After shifting pieces into register, the displacements have mean 15.62 maximum 60.20 pixels. 28 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0030.tif Min = 0.0, max = 12860.0, mean = 919.79, SD = 457.78 Pixel size = 0.127 nm 22.411 electrons (609.14 counts) per unbinned pixel per second 28 frames were saved to Z:\users\KelloggLab\20210513_Cas12K_screen_load2\Cas12k_grid8_0031.tif Running script 3 at item 109-1 (-228.68, -385.74) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 30.37, -71.29 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was -2.9, 8.8 unbinned pixels, 0.26 nm/sec Measured defocus = 1.41 microns changed by -2.41 to target Tilt-induced shift is -18.09, 42.96 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -4.0, 7.7 unbinned pixels, 0.24 nm/sec Measured defocus = -0.85 microns changed by -0.15 to target Tilt-induced shift is -20.84, 49.44 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -1.7, 7.2 unbinned pixels, 0.21 nm/sec Measured defocus = -0.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0001_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0002_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0003_X-1Y+0-0.tif 13:53:37 Refine ZLP: additional offset = 1.4 eV; total offset = 1.4 eV Beam was shifted 0.019 microns 204.547 Running script 3 at item 109-2 (-223.99, -385.36) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 173.647769 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 31.47, -73.65 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -8.6, 4.4 unbinned pixels, 0.27 nm/sec Measured defocus = 1.45 microns changed by -2.65 to target Tilt-induced shift is -22.01, 51.25 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -4.7, 1.9 unbinned pixels, 0.14 nm/sec Measured defocus = -1.01 microns changed by -0.19 to target Tilt-induced shift is -25.06, 60.09 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -3.3, 0.9 unbinned pixels, 0.09 nm/sec Measured defocus = -1.18 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0004_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0005_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0006_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0007_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0008_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0009_X+0Y+1-0.tif 226 Running script 3 at item 109-5 (-210.05, -382.00) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 5.91, -9.91 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -13.5, -1.6 unbinned pixels, 0.37 nm/sec Measured defocus = 0.21 microns changed by -1.61 to target Tilt-induced shift is -27.60, 66.23 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -8.2, 0.2 unbinned pixels, 0.22 nm/sec Measured defocus = -1.30 microns changed by -0.10 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0010_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0011_X+0Y-1-0.tif WARNING: Column valves closed or beam off while acquiring image. Min = 149.0, max = 3280.0, mean = 1588.54, SD = 242.90 Pixel size = 5.91 nm 12.733 electrons (490.54 counts) per unbinned pixel per second Min = 56.0, max = 3151.0, mean = 1581.07, SD = 240.21 Pixel size = 5.91 nm 12.729 electrons (488.23 counts) per unbinned pixel per second Min = 0.0, max = 3379.0, mean = 1573.51, SD = 240.18 Pixel size = 5.91 nm 12.735 electrons (485.90 counts) per unbinned pixel per second Min = 90.0, max = 3566.0, mean = 1581.19, SD = 239.88 Pixel size = 5.91 nm 12.725 electrons (488.27 counts) per unbinned pixel per second Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 56.841533 nm) Running script 3 at item 109-5 (-210.05, -382.00) Dewars are NOT busy filling Column/gun valve state is 1 10.7 -26.2 unbinned pixels; 2.5 56.8 nm along two shift axes; -50.1 -26.8 nm on specimen axes MoveStage 0.025 0.025 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 184.365944 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 0.22, 0.23 unbinned pixels, unfiltered CCC is 0.0051 The average drift between shots was -1.9, 2.5 unbinned pixels, 0.08 nm/sec Measured defocus = -0.00 microns changed by -1.60 to target Tilt-induced shift is -33.34, 78.01 unbinned pixels, unfiltered CCC is 0.0025 The average drift between shots was -2.1, 1.3 unbinned pixels, 0.07 nm/sec Measured defocus = -1.54 microns changed by -0.06 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0012_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0013_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0014_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0015_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0016_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0017_X+0Y+1-0.tif Dewars are NOT busy filling Column/gun valve state is 0 Running script 3 at item 109-5 (-210.05, -382.00) Dewars are NOT busy filling Column/gun valve state is 0 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Group acquire status 1, group ID 866677963, # in group 31, 29 set to acquire ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -1.29, 0.13 unbinned pixels, unfiltered CCC is 0.0473 The average drift between shots was -4.8, -0.8 unbinned pixels, 0.13 nm/sec Measured defocus = -0.01 microns changed by -1.79 to target Tilt-induced shift is -37.29, 86.44 unbinned pixels, unfiltered CCC is 0.0404 The average drift between shots was -5.0, -1.9 unbinned pixels, 0.15 nm/sec Measured defocus = -1.71 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0019_X-1Y-1-0.tif Running script 3 at item 109-5 (-210.05, -382.00) Dewars are NOT busy filling Column/gun valve state is 0 -23.5 -20.5 unbinned pixels; 3.8 1.1 nm along two shift axes; -2.7 2.9 nm on specimen axes MoveStage 0.025 -0.025 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 1.66, -2.95 unbinned pixels, unfiltered CCC is 0.0037 The average drift between shots was -3.3, -0.9 unbinned pixels, 0.10 nm/sec Measured defocus = 0.06 microns changed by -2.06 to target Tilt-induced shift is -41.21, 94.95 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -1.8, -1.6 unbinned pixels, 0.07 nm/sec Measured defocus = -1.88 microns changed by -0.12 to target Tilt-induced shift is -42.24, 99.76 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -1.6, -0.5 unbinned pixels, 0.05 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0021_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0022_X+0Y-1-0.tif WARNING: Column valves closed or beam off while acquiring image. Full View shift offset is set to 0.020, 0.082 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Running script 3 at item 109-5 (-210.61, -383.07) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 484.828978 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 1.76, -4.59 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was -1.8, 4.5 unbinned pixels, 0.13 nm/sec Measured defocus = 0.09 microns changed by -2.29 to target Tilt-induced shift is -43.78, 102.82 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -2.7, 2.4 unbinned pixels, 0.10 nm/sec Measured defocus = -2.03 microns changed by -0.17 to target Tilt-induced shift is -46.34, 110.12 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -2.8, 2.0 unbinned pixels, 0.09 nm/sec Measured defocus = -2.17 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0024_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0025_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0026_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0027_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0028_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0029_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0030_X+1Y+1-0.tif Beam was shifted 0.023 microns 271.125 Running script 3 at item 109-6 (-217.65, -383.57) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 449.266335 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 13.15, -32.71 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -6.9, 0.1 unbinned pixels, 0.18 nm/sec Measured defocus = 0.64 microns changed by -1.64 to target Tilt-induced shift is -20.50, 44.70 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -4.9, -1.0 unbinned pixels, 0.13 nm/sec Measured defocus = -0.89 microns changed by -0.11 to target Tilt-induced shift is -19.80, 49.22 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -4.8, -1.0 unbinned pixels, 0.13 nm/sec Measured defocus = -0.96 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0031_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0032_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0033_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0034_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0035_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0036_X+0Y+1-0.tif 206.563 Running script 3 at item 109-14 (-231.85, -382.30) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 404.793749 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 18.69, -43.30 unbinned pixels, unfiltered CCC is 0.3964 The average drift between shots was -4.2, -2.3 unbinned pixels, 0.12 nm/sec Measured defocus = 0.86 microns changed by -2.06 to target Tilt-induced shift is -21.10, 49.12 unbinned pixels, unfiltered CCC is 0.3687 The average drift between shots was -3.6, 0.2 unbinned pixels, 0.10 nm/sec Measured defocus = -0.97 microns changed by -0.23 to target Tilt-induced shift is -25.58, 59.55 unbinned pixels, unfiltered CCC is 0.3581 The average drift between shots was -2.6, 0.1 unbinned pixels, 0.07 nm/sec Measured defocus = -1.18 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0037_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0038_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0039_X+1Y-1-0.tif Running script 3 at item 114-1 (-515.10, 124.36) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 471.459193 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 139.79, -327.84 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -2.7, 4.0 unbinned pixels, 0.13 nm/sec Measured defocus = 6.44 microns changed by -7.84 to target Tilt-induced shift is -20.08, 46.13 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -1.7, 2.8 unbinned pixels, 0.09 nm/sec Measured defocus = -0.91 microns changed by -0.49 to target Tilt-induced shift is -30.62, 69.63 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -1.3, 3.0 unbinned pixels, 0.09 nm/sec Measured defocus = -1.38 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0040_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0041_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0042_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0043_X-1Y+1-0.tif Beam was shifted 0.035 microns 202.859 Running script 3 at item 114-2 (-508.49, 124.90) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 449.152175 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 82.13, -185.28 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -10.4, -1.9 unbinned pixels, 0.28 nm/sec Measured defocus = 3.66 microns changed by -5.26 to target Tilt-induced shift is -26.03, 57.93 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -3.3, 2.6 unbinned pixels, 0.12 nm/sec Measured defocus = -1.15 microns changed by -0.45 to target Tilt-induced shift is -34.63, 78.11 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -5.3, 1.8 unbinned pixels, 0.15 nm/sec Measured defocus = -1.55 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0044_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0045_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0046_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0047_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0048_X+1Y+1-0.tif 199.016 Running script 3 at item 114-4 (-501.65, 125.45) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 425.220944 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 46.24, -109.75 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -7.1, 3.5 unbinned pixels, 0.22 nm/sec Measured defocus = 2.16 microns changed by -3.96 to target Tilt-induced shift is -32.83, 77.17 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -5.1, 2.9 unbinned pixels, 0.16 nm/sec Measured defocus = -1.52 microns changed by -0.28 to target Tilt-induced shift is -38.57, 88.66 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -3.8, 3.2 unbinned pixels, 0.13 nm/sec Measured defocus = -1.76 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0049_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0050_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0051_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0052_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0053_X+1Y+1-0.tif 198.625 Running script 3 at item 114-15 (-495.11, 128.32) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 292.773525 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -13.09, 33.87 unbinned pixels, unfiltered CCC is 0.0055 The average drift between shots was -11.0, 2.0 unbinned pixels, 0.30 nm/sec Measured defocus = -0.66 microns changed by -1.34 to target Tilt-induced shift is -40.26, 95.20 unbinned pixels, unfiltered CCC is 0.0036 The average drift between shots was -6.6, 3.2 unbinned pixels, 0.19 nm/sec Measured defocus = -1.88 microns changed by -0.12 to target Tilt-induced shift is -43.12, 99.68 unbinned pixels, unfiltered CCC is 0.0042 The average drift between shots was -4.8, 2.8 unbinned pixels, 0.15 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0054_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0055_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0056_X+0Y+1-0.tif 145.391 Running script 3 at item 114-18 (-488.76, 133.53) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 453.109175 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -56.04, 130.95 unbinned pixels, unfiltered CCC is 0.0483 The average drift between shots was -8.4, 4.2 unbinned pixels, 0.25 nm/sec Measured defocus = -2.59 microns changed by 0.39 to target No holes were found! WARNING: Column valves closed or beam off while acquiring image. Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 189.791908 nm) Running script 3 at item 117-1 (-76.86, -325.90) Dewars are NOT busy filling Column/gun valve state is 1 93.3 -14.3 unbinned pixels; 166.2 91.6 nm along two shift axes; -9.3 -188.7 nm on specimen axes MoveStage 0.025 0.025 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 114.048399 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 14.58, -33.97 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -4.7, 6.5 unbinned pixels, 0.22 nm/sec Measured defocus = 0.67 microns changed by -1.67 to target Tilt-induced shift is -19.91, 46.64 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -2.0, 5.3 unbinned pixels, 0.15 nm/sec Measured defocus = -0.92 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0057_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0058_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0059_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0060_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0061_X+0Y+1-0.tif 15:10:55 Refine ZLP: additional offset = 0.8 eV; total offset = 2.1 eV Beam was shifted 0.021 microns 238.031 Running script 3 at item 117-3 (-69.75, -325.41) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 273.044326 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 1.51, -6.32 unbinned pixels, unfiltered CCC is 0.0033 The average drift between shots was -9.4, 0.4 unbinned pixels, 0.26 nm/sec Measured defocus = 0.12 microns changed by -1.32 to target Tilt-induced shift is -23.80, 55.85 unbinned pixels, unfiltered CCC is 0.0025 The average drift between shots was -6.1, -1.4 unbinned pixels, 0.18 nm/sec Measured defocus = -1.10 microns changed by -0.10 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0062_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0063_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0064_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0065_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0066_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0067_X+0Y+1-0.tif 217.297 Running script 3 at item 117-6 (-62.73, -324.86) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 429.979644 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 2.91, -3.49 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -9.2, -1.4 unbinned pixels, 0.25 nm/sec Measured defocus = 0.08 microns changed by -1.48 to target Tilt-induced shift is -28.50, 65.94 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -4.9, 0.4 unbinned pixels, 0.14 nm/sec Measured defocus = -1.30 microns changed by -0.10 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0068_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0069_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0070_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0071_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0072_X+0Y+1-0.tif 196.781 Running script 3 at item 117-8 (-58.17, -324.60) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 266.116115 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 0.95, -0.57 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was -8.0, 2.1 unbinned pixels, 0.22 nm/sec Measured defocus = 0.02 microns changed by -1.62 to target Tilt-induced shift is -33.62, 76.35 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -6.1, 0.8 unbinned pixels, 0.16 nm/sec Measured defocus = -1.51 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 2 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0073_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0074_X+0Y+1-0.tif 109 Running script 3 at item 117-19 (-81.77, -321.65) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 380.636345 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 0.65, -2.76 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -8.0, 1.6 unbinned pixels, 0.23 nm/sec Measured defocus = 0.05 microns changed by -1.85 to target Tilt-induced shift is -35.72, 83.64 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -4.7, 2.3 unbinned pixels, 0.15 nm/sec Measured defocus = -1.65 microns changed by -0.15 to target Tilt-induced shift is -38.06, 89.97 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -3.7, 2.1 unbinned pixels, 0.12 nm/sec Measured defocus = -1.77 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0075_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0076_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0077_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0078_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0079_X-1Y+0-0.tif 186.109 Running script 3 at item 117-20 (-77.12, -321.27) Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 263.301189 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 6.14, -14.14 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -11.5, -0.1 unbinned pixels, 0.31 nm/sec Measured defocus = 0.28 microns changed by -2.28 to target Tilt-induced shift is -39.95, 92.46 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -6.5, 0.2 unbinned pixels, 0.18 nm/sec Measured defocus = -1.83 microns changed by -0.17 to target Tilt-induced shift is -42.79, 101.07 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -5.2, 0.1 unbinned pixels, 0.14 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0080_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0081_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0082_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0083_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0084_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0085_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0086_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0087_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0088_X+1Y+1-0.tif 298.5 Running script 3 at item 117-23 (-70.04, -320.72) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 380.948903 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -5.82, 23.30 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -12.9, -1.4 unbinned pixels, 0.36 nm/sec Measured defocus = -0.43 microns changed by -1.77 to target Tilt-induced shift is -45.91, 106.97 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -5.7, 0.3 unbinned pixels, 0.16 nm/sec Measured defocus = -2.11 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0089_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0090_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0091_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0092_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0093_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0094_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0095_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0096_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0097_X+1Y+1-0.tif 302.375 Running script 3 at item 117-26 (-62.97, -320.20) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -9.88, 23.38 unbinned pixels, unfiltered CCC is 0.0110 The average drift between shots was -11.4, -0.9 unbinned pixels, 0.31 nm/sec Measured defocus = -0.46 microns changed by -0.54 to target Tilt-induced shift is -20.39, 46.49 unbinned pixels, unfiltered CCC is 0.0090 The average drift between shots was -6.2, 0.4 unbinned pixels, 0.17 nm/sec Measured defocus = -0.92 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0098_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0099_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0100_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0101_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0102_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0103_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0104_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0105_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0106_X+1Y+1-0.tif 306.156 Running script 3 at item 117-29 (-55.90, -319.70) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 374.127138 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -6.09, 12.57 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -8.2, 2.4 unbinned pixels, 0.23 nm/sec Measured defocus = -0.25 microns changed by -0.95 to target Tilt-induced shift is -23.41, 55.88 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -5.0, 0.9 unbinned pixels, 0.14 nm/sec Measured defocus = -1.10 microns changed by -0.10 to target Tilt-induced shift is -25.56, 61.23 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -2.9, 0.9 unbinned pixels, 0.09 nm/sec Measured defocus = -1.20 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0107_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0108_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0109_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0110_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0111_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0112_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0113_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0114_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0115_X+1Y+1-0.tif 314.125 Running script 3 at item 117-32 (-49.03, -316.81) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 258.810693 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -16.27, 38.33 unbinned pixels, unfiltered CCC is 0.0137 The average drift between shots was -7.8, 3.7 unbinned pixels, 0.23 nm/sec Measured defocus = -0.76 microns changed by -0.64 to target Tilt-induced shift is -29.63, 69.48 unbinned pixels, unfiltered CCC is 0.0098 The average drift between shots was -5.7, 2.0 unbinned pixels, 0.17 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0116_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0117_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0118_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0119_X+0Y+1-0.tif 166.391 Running script 3 at item 117-64 (-51.61, -312.31) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 427.984045 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -16.98, 44.04 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -7.4, -0.2 unbinned pixels, 0.20 nm/sec Measured defocus = -0.86 microns changed by -0.74 to target Tilt-induced shift is -33.67, 77.71 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -6.4, 1.4 unbinned pixels, 0.18 nm/sec Measured defocus = -1.54 microns changed by -0.06 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0120_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0121_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0122_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0123_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0124_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0125_X+1Y+1-0.tif 208.109 Running script 3 at item 117-66 (-56.34, -312.64) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 358.975778 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -19.17, 39.23 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -7.1, 4.6 unbinned pixels, 0.24 nm/sec Measured defocus = -0.79 microns changed by -1.01 to target Tilt-induced shift is -39.55, 86.90 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -6.0, 1.1 unbinned pixels, 0.17 nm/sec Measured defocus = -1.73 microns changed by -0.07 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0126_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0127_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0128_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0129_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0130_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0131_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0132_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0133_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0134_X+1Y+1-0.tif 305.39 Running script 3 at item 117-69 (-63.40, -313.16) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 329.773703 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -7.85, 20.37 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -7.4, 2.7 unbinned pixels, 0.22 nm/sec Measured defocus = -0.40 microns changed by -1.60 to target Tilt-induced shift is -40.50, 94.91 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -5.8, 2.0 unbinned pixels, 0.17 nm/sec Measured defocus = -1.87 microns changed by -0.13 to target Tilt-induced shift is -44.05, 101.35 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -3.5, 1.1 unbinned pixels, 0.10 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0135_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0136_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0137_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0138_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0139_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0140_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0141_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0142_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0143_X+1Y+1-0.tif 313.828 Running script 3 at item 117-72 (-70.47, -313.68) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 430.789978 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -13.84, 32.25 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -9.5, -0.8 unbinned pixels, 0.26 nm/sec Measured defocus = -0.64 microns changed by -1.56 to target Tilt-induced shift is -44.82, 105.43 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -5.8, 1.4 unbinned pixels, 0.16 nm/sec Measured defocus = -2.08 microns changed by -0.12 to target Tilt-induced shift is -47.83, 110.65 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.9, 1.9 unbinned pixels, 0.12 nm/sec Measured defocus = -2.19 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0144_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0145_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0146_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0147_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0148_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0149_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0150_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0151_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0152_X+1Y+1-0.tif 16:14:48 Refine ZLP: additional offset = 0.4 eV; total offset = 2.5 eV 325.985 Running script 3 at item 117-75 (-77.56, -314.22) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 446.099264 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -9.37, 22.54 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -4.4, -0.0 unbinned pixels, 0.12 nm/sec Measured defocus = -0.44 microns changed by -0.56 to target Tilt-induced shift is -21.19, 46.90 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -5.9, 0.3 unbinned pixels, 0.16 nm/sec Measured defocus = -0.93 microns changed by -0.07 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0153_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0154_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0155_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0156_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0157_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0158_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0159_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0160_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0161_X+1Y+1-0.tif 281.422 Running script 3 at item 117-78 (-82.32, -314.61) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 371.766105 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -0.92, -0.38 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -7.7, 2.5 unbinned pixels, 0.22 nm/sec Measured defocus = -0.00 microns changed by -1.20 to target Tilt-induced shift is -23.08, 57.07 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -4.7, 2.2 unbinned pixels, 0.14 nm/sec Measured defocus = -1.12 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0162_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0163_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0164_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0165_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0166_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0167_X-1Y+0-0.tif 180.157 Running script 3 at item 117-112 (-87.49, -307.94) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -12.24, 32.56 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -10.9, 4.6 unbinned pixels, 0.32 nm/sec Measured defocus = -0.63 microns changed by -0.77 to target Tilt-induced shift is -28.57, 70.22 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -5.6, 2.3 unbinned pixels, 0.16 nm/sec Measured defocus = -1.38 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0168_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0169_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0170_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0171_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0172_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0173_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0174_X+1Y+1-0.tif 245.656 Running script 3 at item 117-114 (-80.37, -307.37) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 406.665443 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -11.96, 26.60 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -7.6, 1.3 unbinned pixels, 0.20 nm/sec Measured defocus = -0.53 microns changed by -1.07 to target Tilt-induced shift is -32.64, 77.39 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -3.1, -0.6 unbinned pixels, 0.08 nm/sec Measured defocus = -1.52 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0175_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0176_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0177_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0178_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0179_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0180_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0181_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0182_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0183_X+1Y+1-0.tif 284.453 Running script 3 at item 117-117 (-73.25, -306.81) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 495.970758 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -13.46, 36.56 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -7.8, 1.7 unbinned pixels, 0.22 nm/sec Measured defocus = -0.71 microns changed by -1.09 to target Tilt-induced shift is -37.54, 86.89 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -6.2, -0.4 unbinned pixels, 0.17 nm/sec Measured defocus = -1.72 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0184_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0185_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0186_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0187_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0188_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0189_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0190_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0191_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0192_X+1Y+1-0.tif 301.25 Running script 3 at item 117-120 (-66.19, -306.30) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 418.208442 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -28.51, 62.97 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -8.1, 0.4 unbinned pixels, 0.22 nm/sec Measured defocus = -1.25 microns changed by -0.75 to target Tilt-induced shift is -43.49, 99.17 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.7, 1.3 unbinned pixels, 0.15 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0193_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0194_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0195_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0196_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0197_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0198_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0199_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0200_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0201_X+1Y+1-0.tif 300.547 Running script 3 at item 117-123 (-59.13, -305.78) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 413.82605 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -17.75, 43.79 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -8.9, 3.2 unbinned pixels, 0.26 nm/sec Measured defocus = -0.86 microns changed by -1.34 to target Tilt-induced shift is -44.77, 104.78 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.0, 2.4 unbinned pixels, 0.15 nm/sec Measured defocus = -2.07 microns changed by -0.13 to target Tilt-induced shift is -48.35, 110.69 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -3.7, 1.6 unbinned pixels, 0.11 nm/sec Measured defocus = -2.19 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0202_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0203_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0204_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0205_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0206_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0207_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0208_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0209_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0210_X+1Y+1-0.tif 315.781 Running script 3 at item 117-126 (-52.12, -305.31) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 401.300867 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -23.90, 57.95 unbinned pixels, unfiltered CCC is 0.0041 The average drift between shots was -9.8, 2.3 unbinned pixels, 0.28 nm/sec Measured defocus = -1.14 microns changed by 0.14 to target Tilt-induced shift is -21.46, 51.99 unbinned pixels, unfiltered CCC is 0.0047 The average drift between shots was -4.2, 1.8 unbinned pixels, 0.12 nm/sec Measured defocus = -1.02 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0211_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0212_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0213_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0214_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0215_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0216_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0217_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0218_X+0Y+1-0.tif 261.062 Running script 3 at item 117-163 (-54.93, -298.38) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 233.092291 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -34.30, 86.65 unbinned pixels, unfiltered CCC is 0.0032 The average drift between shots was -7.1, -1.5 unbinned pixels, 0.20 nm/sec Measured defocus = -1.69 microns changed by 0.49 to target Tilt-induced shift is -26.06, 61.78 unbinned pixels, unfiltered CCC is 0.0043 The average drift between shots was -4.8, 1.5 unbinned pixels, 0.14 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0219_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0220_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0221_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0222_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0223_X+0Y+1-0.tif 184.734 Running script 3 at item 117-165 (-59.56, -298.73) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 291.885506 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -37.45, 80.94 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -6.6, 4.4 unbinned pixels, 0.21 nm/sec Measured defocus = -1.62 microns changed by 0.22 to target Tilt-induced shift is -30.64, 72.53 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.3, 2.3 unbinned pixels, 0.15 nm/sec Measured defocus = -1.43 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0224_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0225_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0226_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0227_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0228_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0229_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0230_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0231_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0232_X+1Y+1-0.tif 305.625 Running script 3 at item 117-168 (-66.61, -299.26) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 443.462491 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -24.97, 59.84 unbinned pixels, unfiltered CCC is 0.0049 The average drift between shots was -7.7, 2.4 unbinned pixels, 0.22 nm/sec Measured defocus = -1.18 microns changed by -0.42 to target Tilt-induced shift is -34.20, 78.71 unbinned pixels, unfiltered CCC is 0.0037 The average drift between shots was -4.7, 1.8 unbinned pixels, 0.14 nm/sec Measured defocus = -1.56 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0233_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0234_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0235_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0236_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0237_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0238_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0239_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0240_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0241_X+1Y+1-0.tif 306.25 Running script 3 at item 117-171 (-73.69, -299.77) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 393.875371 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -30.14, 69.59 unbinned pixels, unfiltered CCC is 0.0077 The average drift between shots was -8.3, 1.8 unbinned pixels, 0.24 nm/sec Measured defocus = -1.38 microns changed by -0.42 to target Tilt-induced shift is -39.12, 91.07 unbinned pixels, unfiltered CCC is 0.0070 The average drift between shots was -4.5, 0.4 unbinned pixels, 0.12 nm/sec Measured defocus = -1.80 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0242_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0243_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0244_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0245_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0246_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0247_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0248_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0249_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0250_X+1Y+1-0.tif 292.515 Running script 3 at item 117-174 (-80.79, -300.32) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 287.363477 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -28.77, 66.71 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -7.3, 0.9 unbinned pixels, 0.19 nm/sec Measured defocus = -1.32 microns changed by -0.68 to target Tilt-induced shift is -43.05, 97.93 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.4, 1.6 unbinned pixels, 0.15 nm/sec Measured defocus = -1.94 microns changed by -0.06 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0251_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0252_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0253_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0254_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0255_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0256_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0257_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0258_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0259_X+1Y+1-0.tif 17:15:14 Refine ZLP: additional offset = 0.9 eV; total offset = 3.4 eV 309.265 Running script 3 at item 117-177 (-87.93, -300.95) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 368.615378 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -26.14, 55.58 unbinned pixels, unfiltered CCC is 0.0059 The average drift between shots was -8.7, 3.5 unbinned pixels, 0.26 nm/sec Measured defocus = -1.11 microns changed by -1.09 to target Tilt-induced shift is -45.83, 106.53 unbinned pixels, unfiltered CCC is 0.0047 The average drift between shots was -6.3, 2.0 unbinned pixels, 0.18 nm/sec Measured defocus = -2.11 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0260_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0261_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0262_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0263_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0264_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0265_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0266_X+1Y+1-0.tif 235.781 Running script 3 at item 117-211 (-78.71, -295.42) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 241.847868 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -30.66, 70.63 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -7.7, 1.1 unbinned pixels, 0.21 nm/sec Measured defocus = -1.40 microns changed by 0.40 to target Tilt-induced shift is -24.61, 53.47 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -5.9, 0.4 unbinned pixels, 0.16 nm/sec Measured defocus = -1.07 microns changed by 0.07 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0267_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0268_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0269_X+0Y+1-0.tif 137.829 Running script 3 at item 117-214 (-71.62, -294.89) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 256.905385 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -30.80, 73.94 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -11.0, -0.2 unbinned pixels, 0.30 nm/sec Measured defocus = -1.45 microns changed by 0.25 to target Tilt-induced shift is -26.28, 61.06 unbinned pixels, unfiltered CCC is 0.0030 The average drift between shots was -5.1, 0.7 unbinned pixels, 0.14 nm/sec Measured defocus = -1.21 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0270_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0271_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0272_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0273_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0274_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0275_X+1Y+1-0.tif 209.891 Running script 3 at item 117-217 (-64.69, -292.03) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 265.892032 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -40.00, 87.44 unbinned pixels, unfiltered CCC is 0.0068 The average drift between shots was -8.3, 3.2 unbinned pixels, 0.24 nm/sec Measured defocus = -1.75 microns changed by 0.35 to target Tilt-induced shift is -30.77, 73.45 unbinned pixels, unfiltered CCC is 0.0075 The average drift between shots was -5.5, 1.5 unbinned pixels, 0.16 nm/sec Measured defocus = -1.45 microns changed by 0.05 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0276_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0277_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0278_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0279_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0280_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0281_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0282_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0283_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0284_X+1Y+1-0.tif 284.625 Running script 3 at item 117-220 (-57.62, -291.50) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 323.251759 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -47.94, 110.50 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -8.0, 0.3 unbinned pixels, 0.21 nm/sec Measured defocus = -2.19 microns changed by 0.59 to target Tilt-induced shift is -34.70, 83.78 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -2.9, 1.6 unbinned pixels, 0.09 nm/sec Measured defocus = -1.65 microns changed by 0.05 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0285_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0286_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0287_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0288_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0289_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0290_X-1Y+1-0.tif 211.781 Running script 3 at item 119-1 (-312.04, -417.13) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 14.31, -32.28 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -1.7, 8.7 unbinned pixels, 0.24 nm/sec Measured defocus = 0.64 microns changed by -2.44 to target Tilt-induced shift is -35.81, 86.17 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -2.1, 6.3 unbinned pixels, 0.18 nm/sec Measured defocus = -1.69 microns changed by -0.11 to target Tilt-induced shift is -39.30, 91.42 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -1.9, 4.7 unbinned pixels, 0.14 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0291_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0292_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0293_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0294_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0295_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0296_X+1Y+1-0.tif Beam was shifted 0.041 microns 261.875 Running script 3 at item 119-2 (-305.10, -416.62) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -1.05, 9.67 unbinned pixels, unfiltered CCC is 0.0248 The average drift between shots was -8.6, 2.3 unbinned pixels, 0.24 nm/sec Measured defocus = -0.17 microns changed by -1.83 to target Tilt-induced shift is -40.83, 93.86 unbinned pixels, unfiltered CCC is 0.0136 The average drift between shots was -4.5, 3.9 unbinned pixels, 0.16 nm/sec Measured defocus = -1.86 microns changed by -0.14 to target Tilt-induced shift is -42.99, 99.21 unbinned pixels, unfiltered CCC is 0.0130 The average drift between shots was -3.3, 3.9 unbinned pixels, 0.14 nm/sec Measured defocus = -1.96 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0297_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0298_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0299_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0300_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0301_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0302_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0303_X+1Y+1-0.tif 243.11 Running script 3 at item 119-14 (-298.21, -413.83) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 490.584578 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -40.94, 97.02 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -7.3, 0.5 unbinned pixels, 0.20 nm/sec Measured defocus = -1.91 microns changed by -0.29 to target Tilt-induced shift is -46.04, 108.35 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.4, 2.9 unbinned pixels, 0.16 nm/sec Measured defocus = -2.14 microns changed by -0.06 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0304_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0305_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0306_X+0Y+1-0.tif 138.406 Running script 3 at item 119-17 (-289.17, -408.52) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 247.877149 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -60.72, 149.25 unbinned pixels, unfiltered CCC is 0.0048 The average drift between shots was -8.9, -0.9 unbinned pixels, 0.24 nm/sec Measured defocus = -2.93 microns changed by 1.93 to target Tilt-induced shift is -24.85, 61.75 unbinned pixels, unfiltered CCC is 0.0095 The average drift between shots was -4.9, 3.6 unbinned pixels, 0.17 nm/sec Measured defocus = -1.21 microns changed by 0.21 to target Tilt-induced shift is -22.37, 50.62 unbinned pixels, unfiltered CCC is 0.0101 The average drift between shots was -4.9, 2.8 unbinned pixels, 0.16 nm/sec Measured defocus = -1.00 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0307_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0308_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0309_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0310_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0311_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0312_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0313_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0314_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0315_X+1Y+1-0.tif 298.281 Running script 3 at item 119-20 (-296.16, -408.98) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 403.168174 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -43.25, 99.60 unbinned pixels, unfiltered CCC is 0.0030 The average drift between shots was -7.3, 1.4 unbinned pixels, 0.20 nm/sec Measured defocus = -1.97 microns changed by 0.77 to target Tilt-induced shift is -26.59, 62.17 unbinned pixels, unfiltered CCC is 0.0031 The average drift between shots was -6.2, 0.2 unbinned pixels, 0.17 nm/sec Measured defocus = -1.23 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0316_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0317_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0318_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0319_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0320_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0321_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0322_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0323_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0324_X+1Y+1-0.tif 294.594 Running script 3 at item 119-23 (-303.12, -409.46) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 217.167266 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -13.43, 31.68 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -8.8, 7.4 unbinned pixels, 0.31 nm/sec Measured defocus = -0.62 microns changed by -0.78 to target Tilt-induced shift is -30.69, 68.92 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -4.9, 2.9 unbinned pixels, 0.15 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0325_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0326_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0327_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0328_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0329_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0330_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0331_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0332_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0333_X+1Y+1-0.tif 303.812 Running script 3 at item 119-26 (-310.05, -409.97) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -14.47, 33.85 unbinned pixels, unfiltered CCC is 0.0039 The average drift between shots was -13.2, 2.9 unbinned pixels, 0.37 nm/sec Measured defocus = -0.67 microns changed by -0.93 to target Tilt-induced shift is -32.74, 76.34 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -6.1, 2.4 unbinned pixels, 0.18 nm/sec Measured defocus = -1.51 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0334_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0335_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0336_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0337_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0338_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0339_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0340_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0341_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0342_X+1Y+1-0.tif 305.219 Running script 3 at item 119-29 (-314.68, -410.34) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -0.94, 5.78 unbinned pixels, unfiltered CCC is 0.0055 The average drift between shots was -17.0, 3.8 unbinned pixels, 0.47 nm/sec Measured defocus = -0.10 microns changed by -1.70 to target Tilt-induced shift is -34.85, 84.33 unbinned pixels, unfiltered CCC is 0.0039 The average drift between shots was -9.2, 2.2 unbinned pixels, 0.25 nm/sec Measured defocus = -1.66 microns changed by -0.14 to target Tilt-induced shift is -38.82, 90.16 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -5.1, 1.7 unbinned pixels, 0.14 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0343_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0344_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0345_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0346_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0347_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0348_X-1Y+0-0.tif 217.516 Running script 3 at item 119-45 (-284.64, -405.88) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 224.625714 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -72.68, 168.20 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -10.7, 7.6 unbinned pixels, 0.35 nm/sec Measured defocus = -3.33 microns changed by 1.33 to target Tilt-induced shift is -48.15, 111.14 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -5.9, 2.6 unbinned pixels, 0.17 nm/sec Measured defocus = -2.20 microns changed by 0.20 to target Tilt-induced shift is -44.84, 103.17 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -3.6, 2.4 unbinned pixels, 0.12 nm/sec Measured defocus = -2.04 microns changed by 0.04 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0349_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0350_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0351_X+0Y+1-0.tif 133.813 Running script 3 at item 119-62 (-317.45, -403.49) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 341.643605 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 15.77, -30.65 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -9.6, -5.5 unbinned pixels, 0.30 nm/sec Measured defocus = 0.62 microns changed by -2.82 to target Tilt-induced shift is -45.88, 105.11 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -6.1, 1.0 unbinned pixels, 0.16 nm/sec Measured defocus = -2.08 microns changed by -0.12 to target Tilt-induced shift is -48.58, 110.15 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -4.1, 0.9 unbinned pixels, 0.11 nm/sec Measured defocus = -2.19 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0352_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0353_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0354_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0355_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0356_X-1Y+0-0.tif 172.531 Running script 3 at item 119-63 (-312.76, -403.11) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 320.744562 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -1.16, 3.36 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -7.0, 1.0 unbinned pixels, 0.19 nm/sec Measured defocus = -0.06 microns changed by -0.94 to target Tilt-induced shift is -19.74, 46.72 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -5.2, 2.6 unbinned pixels, 0.16 nm/sec Measured defocus = -0.92 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0357_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0358_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0359_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0360_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0361_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0362_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0363_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0364_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0365_X+1Y+1-0.tif 18:19:24 Refine ZLP: additional offset = 0.4 eV; total offset = 3.7 eV 318.797 Running script 3 at item 119-66 (-305.76, -402.58) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 489.24896 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -25.66, 49.46 unbinned pixels, unfiltered CCC is 0.0608 The average drift between shots was -9.1, 4.9 unbinned pixels, 0.28 nm/sec Measured defocus = -1.01 microns changed by -0.19 to target Tilt-induced shift is -26.55, 59.75 unbinned pixels, unfiltered CCC is 0.0547 The average drift between shots was -5.2, 0.3 unbinned pixels, 0.14 nm/sec Measured defocus = -1.19 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0366_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0367_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0368_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0369_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0370_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0371_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0372_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0373_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0374_X+1Y+1-0.tif 305.547 Running script 3 at item 119-69 (-298.90, -402.10) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 200.221014 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -28.09, 63.28 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -8.6, 4.8 unbinned pixels, 0.27 nm/sec Measured defocus = -1.26 microns changed by -0.14 to target Tilt-induced shift is -29.75, 70.68 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -5.3, 4.1 unbinned pixels, 0.18 nm/sec Measured defocus = -1.39 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0375_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0376_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0377_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0378_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0379_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0380_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0381_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0382_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0383_X+1Y+1-0.tif 294.453 Running script 3 at item 119-72 (-291.92, -401.62) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 397.03853 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -58.97, 134.61 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -7.0, 1.6 unbinned pixels, 0.19 nm/sec Measured defocus = -2.67 microns changed by 1.07 to target Tilt-induced shift is -38.74, 87.27 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -4.7, 2.0 unbinned pixels, 0.14 nm/sec Measured defocus = -1.73 microns changed by 0.13 to target Tilt-induced shift is -35.23, 80.96 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -3.5, 1.3 unbinned pixels, 0.11 nm/sec Measured defocus = -1.60 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0384_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0385_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0386_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0387_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0388_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0389_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0390_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0391_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0392_X+1Y+1-0.tif 292.188 Running script 3 at item 119-75 (-284.91, -401.16) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 198.416276 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -76.13, 179.79 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -7.1, 6.6 unbinned pixels, 0.27 nm/sec Measured defocus = -3.55 microns changed by 1.75 to target Tilt-induced shift is -42.68, 98.13 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -5.3, 0.9 unbinned pixels, 0.14 nm/sec Measured defocus = -1.94 microns changed by 0.14 to target Tilt-induced shift is -40.65, 92.29 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -3.1, 0.6 unbinned pixels, 0.09 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0393_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0394_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0395_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0396_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0397_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0398_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0399_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0400_X+0Y+1-0.tif 274.063 Running script 3 at item 119-111 (-287.66, -394.26) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 221.313431 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -75.93, 181.97 unbinned pixels, unfiltered CCC is 0.0067 The average drift between shots was -10.3, -4.1 unbinned pixels, 0.30 nm/sec Measured defocus = -3.58 microns changed by 1.58 to target Tilt-induced shift is -46.61, 107.81 unbinned pixels, unfiltered CCC is 0.0159 The average drift between shots was -5.1, 1.6 unbinned pixels, 0.14 nm/sec Measured defocus = -2.13 microns changed by 0.13 to target Tilt-induced shift is -43.98, 101.23 unbinned pixels, unfiltered CCC is 0.0187 The average drift between shots was -4.3, 0.7 unbinned pixels, 0.12 nm/sec Measured defocus = -2.00 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0401_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0402_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0403_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0404_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0405_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0406_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0407_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0408_X+0Y+1-0.tif 275.062 Running script 3 at item 119-114 (-294.65, -394.73) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 279.917732 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -63.08, 148.36 unbinned pixels, unfiltered CCC is 0.0040 The average drift between shots was -9.8, -6.3 unbinned pixels, 0.31 nm/sec Measured defocus = -2.93 microns changed by 0.73 to target Tilt-induced shift is -49.12, 112.49 unbinned pixels, unfiltered CCC is 0.0035 The average drift between shots was -5.0, 0.4 unbinned pixels, 0.14 nm/sec Measured defocus = -2.23 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0409_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0410_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0411_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0412_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0413_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0414_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0415_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0416_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0417_X+1Y+1-0.tif 288.922 Running script 3 at item 119-117 (-301.50, -395.20) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 290.081022 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -37.93, 85.37 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -7.1, 3.5 unbinned pixels, 0.21 nm/sec Measured defocus = -1.70 microns changed by 0.70 to target Tilt-induced shift is -24.16, 58.33 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -6.0, 0.9 unbinned pixels, 0.17 nm/sec Measured defocus = -1.15 microns changed by 0.15 to target Tilt-induced shift is -21.55, 49.26 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -3.9, 1.3 unbinned pixels, 0.11 nm/sec Measured defocus = -0.98 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0418_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0419_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0420_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0421_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0422_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0423_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0424_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0425_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0426_X+1Y+1-0.tif 318.094 Running script 3 at item 119-120 (-308.51, -395.73) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 390.724711 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -9.27, 20.60 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -9.3, 6.9 unbinned pixels, 0.32 nm/sec Measured defocus = -0.41 microns changed by -0.79 to target Tilt-induced shift is -25.84, 59.30 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -4.3, 1.8 unbinned pixels, 0.13 nm/sec Measured defocus = -1.17 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0427_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0428_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0429_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0430_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0431_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0432_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0433_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0434_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0435_X+1Y+1-0.tif 304.14 Running script 3 at item 119-123 (-315.50, -396.24) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 331.948293 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -8.87, 21.06 unbinned pixels, unfiltered CCC is 0.0096 The average drift between shots was -9.2, 0.8 unbinned pixels, 0.26 nm/sec Measured defocus = -0.41 microns changed by -0.99 to target Tilt-induced shift is -28.36, 65.34 unbinned pixels, unfiltered CCC is 0.0079 The average drift between shots was -5.9, 2.9 unbinned pixels, 0.18 nm/sec Measured defocus = -1.29 microns changed by -0.11 to target Tilt-induced shift is -29.82, 71.59 unbinned pixels, unfiltered CCC is 0.0072 The average drift between shots was -5.8, 1.8 unbinned pixels, 0.17 nm/sec Measured defocus = -1.41 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0436_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0437_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0438_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0439_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0440_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0441_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0442_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0443_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0444_X+1Y+1-0.tif 306.094 Running script 3 at item 119-126 (-320.20, -396.62) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 308.381122 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 11.83, -25.32 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -10.2, 1.9 unbinned pixels, 0.28 nm/sec Measured defocus = 0.51 microns changed by -2.11 to target Tilt-induced shift is -32.34, 73.70 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.9, 3.6 unbinned pixels, 0.17 nm/sec Measured defocus = -1.46 microns changed by -0.14 to target Tilt-induced shift is -34.45, 80.03 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -3.8, 2.0 unbinned pixels, 0.12 nm/sec Measured defocus = -1.58 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0445_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0446_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0447_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0448_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0449_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0450_X-1Y+0-0.tif 195.094 Running script 3 at item 119-161 (-320.64, -389.56) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 314.79337 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 0.73, -2.30 unbinned pixels, unfiltered CCC is 0.0131 The average drift between shots was -10.7, 0.4 unbinned pixels, 0.29 nm/sec Measured defocus = 0.04 microns changed by -1.84 to target Tilt-induced shift is -35.76, 84.05 unbinned pixels, unfiltered CCC is 0.0070 The average drift between shots was -5.4, 2.1 unbinned pixels, 0.16 nm/sec Measured defocus = -1.66 microns changed by -0.14 to target Tilt-induced shift is -38.85, 90.54 unbinned pixels, unfiltered CCC is 0.0069 The average drift between shots was -3.2, 1.8 unbinned pixels, 0.10 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0451_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0452_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0453_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0454_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0455_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0456_X-1Y+0-0.tif 200.532 Running script 3 at item 119-163 (-315.92, -389.19) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 462.848658 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -12.57, 36.21 unbinned pixels, unfiltered CCC is 0.0152 The average drift between shots was -9.4, 0.7 unbinned pixels, 0.26 nm/sec Measured defocus = -0.69 microns changed by -1.31 to target Tilt-induced shift is -40.15, 93.06 unbinned pixels, unfiltered CCC is 0.0114 The average drift between shots was -5.6, 10.7 unbinned pixels, 0.34 nm/sec Measured defocus = -1.84 microns changed by -0.16 to target Tilt-induced shift is -43.15, 99.26 unbinned pixels, unfiltered CCC is 0.0108 The average drift between shots was -3.8, 4.4 unbinned pixels, 0.17 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0457_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0458_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0459_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0460_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0461_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0462_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0463_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0464_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0465_X+1Y+1-0.tif 303.594 Running script 3 at item 119-166 (-308.91, -388.68) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 324.892382 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -17.31, 42.91 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -9.3, 1.4 unbinned pixels, 0.27 nm/sec Measured defocus = -0.84 microns changed by -1.36 to target Tilt-induced shift is -46.86, 108.43 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -6.3, 1.4 unbinned pixels, 0.18 nm/sec Measured defocus = -2.14 microns changed by -0.06 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0466_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0467_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0468_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0469_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0470_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0471_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0472_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0473_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0474_X+1Y+1-0.tif 19:21:42 Refine ZLP: additional offset = 0.2 eV; total offset = 3.9 eV 322.625 Running script 3 at item 119-169 (-301.90, -388.17) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 205.963302 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -54.80, 124.97 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -7.5, 3.3 unbinned pixels, 0.23 nm/sec Measured defocus = -2.48 microns changed by 1.48 to target Tilt-induced shift is -26.41, 59.63 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -5.3, 1.4 unbinned pixels, 0.15 nm/sec Measured defocus = -1.18 microns changed by 0.18 to target Tilt-induced shift is -22.16, 50.93 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -3.9, 1.3 unbinned pixels, 0.11 nm/sec Measured defocus = -1.01 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0475_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0476_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0477_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0478_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0479_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0480_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0481_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0482_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0483_X+1Y+1-0.tif 311.656 Running script 3 at item 119-172 (-295.03, -387.68) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 363.393288 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -50.12, 114.66 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -8.4, 5.2 unbinned pixels, 0.27 nm/sec Measured defocus = -2.27 microns changed by 1.07 to target Tilt-induced shift is -30.22, 68.25 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -5.0, 2.1 unbinned pixels, 0.15 nm/sec Measured defocus = -1.36 microns changed by 0.16 to target Tilt-induced shift is -26.52, 61.34 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -4.2, 0.4 unbinned pixels, 0.12 nm/sec Measured defocus = -1.21 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0484_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0485_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0486_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0487_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0488_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0489_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0490_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0491_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0492_X+1Y+1-0.tif 302.032 Running script 3 at item 119-175 (-290.40, -387.36) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 361.178567 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -88.63, 204.69 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -8.4, 0.9 unbinned pixels, 0.23 nm/sec Measured defocus = -4.05 microns changed by 2.65 to target Tilt-induced shift is -37.44, 85.74 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.2, 1.4 unbinned pixels, 0.14 nm/sec Measured defocus = -1.70 microns changed by 0.30 to target Tilt-induced shift is -30.08, 73.08 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -4.5, 0.5 unbinned pixels, 0.13 nm/sec Measured defocus = -1.43 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0493_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0494_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0495_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0496_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0497_X+0Y+1-0.tif 191.781 Running script 3 at item 119-208 (-293.10, -380.48) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 340.072223 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -67.81, 154.41 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -8.0, 4.9 unbinned pixels, 0.26 nm/sec Measured defocus = -3.06 microns changed by 1.46 to target Tilt-induced shift is -39.12, 91.57 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -5.8, 0.6 unbinned pixels, 0.16 nm/sec Measured defocus = -1.81 microns changed by 0.21 to target Tilt-induced shift is -35.51, 82.28 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -4.5, 0.8 unbinned pixels, 0.13 nm/sec Measured defocus = -1.63 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0498_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0499_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0500_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0501_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0502_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0503_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0504_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0505_X+0Y+1-0.tif 275.813 Running script 3 at item 119-211 (-299.99, -380.98) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 223.09441 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -53.14, 123.38 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -10.7, 2.0 unbinned pixels, 0.30 nm/sec Measured defocus = -2.44 microns changed by 0.64 to target Tilt-induced shift is -40.55, 94.85 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -6.2, 0.9 unbinned pixels, 0.17 nm/sec Measured defocus = -1.87 microns changed by 0.07 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0506_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0507_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0508_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0509_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0510_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0511_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0512_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0513_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0514_X+1Y+1-0.tif 298.922 Running script 3 at item 119-214 (-306.97, -381.51) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 180.761589 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -33.70, 79.40 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -8.5, -0.5 unbinned pixels, 0.23 nm/sec Measured defocus = -1.57 microns changed by -0.43 to target Tilt-induced shift is -41.92, 98.65 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -6.3, 1.5 unbinned pixels, 0.17 nm/sec Measured defocus = -1.95 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0515_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0516_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0517_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0518_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0519_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0520_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0521_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0522_X+1Y+1-0.tif 269.188 Running script 3 at item 119-217 (-313.88, -384.31) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 390.750493 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -22.98, 54.02 unbinned pixels, unfiltered CCC is 0.0036 The average drift between shots was -10.3, 0.9 unbinned pixels, 0.28 nm/sec Measured defocus = -1.07 microns changed by -1.13 to target Tilt-induced shift is -46.38, 106.12 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -5.0, 3.2 unbinned pixels, 0.16 nm/sec Measured defocus = -2.10 microns changed by -0.10 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0523_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0524_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0525_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0526_X+1Y+1-0.tif 164.078 Running script 3 at item 120-1 (-489.19, -396.86) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 426.593286 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 129.88, -302.05 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -3.2, 0.8 unbinned pixels, 0.09 nm/sec Measured defocus = 5.94 microns changed by -6.94 to target Tilt-induced shift is -14.09, 32.08 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -1.7, 1.8 unbinned pixels, 0.07 nm/sec Measured defocus = -0.64 microns changed by -0.36 to target Tilt-induced shift is -20.79, 49.30 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -1.4, 1.8 unbinned pixels, 0.06 nm/sec Measured defocus = -0.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0527_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0528_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0529_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0530_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0531_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0532_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0533_X+1Y+1-0.tif Beam was shifted 0.023 microns 282.015 Running script 3 at item 120-3 (-482.78, -396.52) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 240.574571 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 43.49, -92.36 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -8.6, -1.4 unbinned pixels, 0.24 nm/sec Measured defocus = 1.85 microns changed by -3.05 to target Tilt-induced shift is -18.02, 45.65 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -2.6, 4.6 unbinned pixels, 0.14 nm/sec Measured defocus = -0.89 microns changed by -0.31 to target Tilt-induced shift is -26.13, 58.71 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -2.6, 2.8 unbinned pixels, 0.11 nm/sec Measured defocus = -1.17 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0534_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0535_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0536_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0537_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0538_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0539_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0540_X+1Y+1-0.tif 251.453 Running script 3 at item 120-4 (-476.38, -393.80) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 477.635874 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 7.78, -16.46 unbinned pixels, unfiltered CCC is 0.0271 The average drift between shots was -10.4, -5.3 unbinned pixels, 0.32 nm/sec Measured defocus = 0.33 microns changed by -1.73 to target Tilt-induced shift is -26.51, 63.19 unbinned pixels, unfiltered CCC is 0.0166 The average drift between shots was -7.5, 2.2 unbinned pixels, 0.22 nm/sec Measured defocus = -1.24 microns changed by -0.16 to target Tilt-induced shift is -30.38, 70.68 unbinned pixels, unfiltered CCC is 0.0164 The average drift between shots was -4.8, 2.2 unbinned pixels, 0.15 nm/sec Measured defocus = -1.40 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0541_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0542_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0543_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0544_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0545_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0546_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0547_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0548_X+1Y+1-0.tif 276.875 Running script 3 at item 120-11 (-493.80, -392.49) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 105.02, -243.73 unbinned pixels, unfiltered CCC is 0.0210 The average drift between shots was -13.1, 6.0 unbinned pixels, 0.39 nm/sec Measured defocus = 4.80 microns changed by -6.40 to target Tilt-induced shift is -27.42, 63.31 unbinned pixels, unfiltered CCC is 0.0271 The average drift between shots was -7.2, 3.9 unbinned pixels, 0.23 nm/sec Measured defocus = -1.25 microns changed by -0.35 to target Tilt-induced shift is -34.77, 80.36 unbinned pixels, unfiltered CCC is 0.0265 The average drift between shots was -5.1, 3.8 unbinned pixels, 0.18 nm/sec Measured defocus = -1.59 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0549_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0550_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0551_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0552_X-1Y+0-0.tif 174.547 Running script 3 at item 120-21 (-469.99, -391.12) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 442.569154 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -69.90, 161.64 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -10.1, 3.5 unbinned pixels, 0.30 nm/sec Measured defocus = -3.20 microns changed by 1.40 to target Tilt-induced shift is -42.45, 97.74 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -6.5, 1.5 unbinned pixels, 0.19 nm/sec Measured defocus = -1.93 microns changed by 0.13 to target Tilt-induced shift is -39.96, 92.00 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -4.5, 1.7 unbinned pixels, 0.13 nm/sec Measured defocus = -1.82 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0553_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0554_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0555_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0556_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0557_X+0Y+1-0.tif 191.563 Running script 3 at item 120-23 (-463.50, -388.45) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 356.824679 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -104.33, 241.30 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -11.1, 5.3 unbinned pixels, 0.33 nm/sec Measured defocus = -4.78 microns changed by 2.78 to target Tilt-induced shift is -51.25, 117.61 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -6.7, 4.1 unbinned pixels, 0.21 nm/sec Measured defocus = -2.33 microns changed by 0.33 to target Tilt-induced shift is -44.33, 102.69 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -4.9, 2.3 unbinned pixels, 0.15 nm/sec Measured defocus = -2.03 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0558_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0559_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0560_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0561_X+0Y+1-0.tif 166.453 Running script 3 at item 120-30 (-483.30, -389.52) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 237.912856 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 51.71, -113.15 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -8.6, -1.3 unbinned pixels, 0.24 nm/sec Measured defocus = 2.25 microns changed by -4.45 to target Tilt-induced shift is -41.18, 95.84 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -7.2, 12.3 unbinned pixels, 0.39 nm/sec Measured defocus = -1.89 microns changed by -0.31 to target Tilt-induced shift is -46.00, 105.64 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -6.0, 11.1 unbinned pixels, 0.35 nm/sec Measured defocus = -2.09 microns changed by -0.11 to target Tilt-induced shift is -47.95, 111.08 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -3.9, 7.7 unbinned pixels, 0.24 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0562_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0563_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0564_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0565_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0566_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0567_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0568_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0569_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0570_X+1Y+1-0.tif 319.765 Running script 3 at item 120-33 (-489.85, -389.90) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 244.962414 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 93.74, -216.99 unbinned pixels, unfiltered CCC is 0.0053 The average drift between shots was -8.7, -1.8 unbinned pixels, 0.24 nm/sec Measured defocus = 4.27 microns changed by -5.27 to target Tilt-induced shift is -11.18, 25.20 unbinned pixels, unfiltered CCC is 0.0181 The average drift between shots was -6.8, 4.9 unbinned pixels, 0.22 nm/sec Measured defocus = -0.50 microns changed by -0.50 to target Tilt-induced shift is -21.78, 49.08 unbinned pixels, unfiltered CCC is 0.0144 The average drift between shots was -4.0, 3.2 unbinned pixels, 0.14 nm/sec Measured defocus = -0.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0571_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0572_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0573_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0574_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0575_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0576_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0577_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0578_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0579_X+1Y+1-0.tif 20:26:19 Refine ZLP: additional offset = -0.5 eV; total offset = 3.4 eV 326.485 Running script 3 at item 120-45 (-476.84, -386.76) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 413.544371 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -11.14, 36.68 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -7.0, -20.5 unbinned pixels, 0.60 nm/sec Measured defocus = -0.69 microns changed by -0.51 to target Tilt-induced shift is -18.12, 41.30 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -5.9, 3.8 unbinned pixels, 0.19 nm/sec Measured defocus = -0.82 microns changed by -0.38 to target Tilt-induced shift is -25.04, 57.03 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -5.0, 3.1 unbinned pixels, 0.16 nm/sec Measured defocus = -1.13 microns changed by -0.07 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0580_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0581_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0582_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0583_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0584_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0585_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0586_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0587_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0588_X+1Y+1-0.tif 304.5 Running script 3 at item 120-48 (-470.11, -386.43) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 210.946428 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -54.38, 134.16 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -13.9, -6.7 unbinned pixels, 0.42 nm/sec Measured defocus = -2.63 microns changed by 1.23 to target Tilt-induced shift is -33.12, 75.21 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -4.9, 2.7 unbinned pixels, 0.15 nm/sec Measured defocus = -1.49 microns changed by 0.09 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0589_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0590_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0591_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0592_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0593_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0594_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0595_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0596_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0597_X+1Y+1-0.tif 299.625 Running script 3 at item 120-54 (-463.82, -383.78) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 228.651396 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -122.79, 291.49 unbinned pixels, unfiltered CCC is 0.0032 The average drift between shots was -7.3, -14.4 unbinned pixels, 0.44 nm/sec Measured defocus = -5.76 microns changed by 4.16 to target Tilt-induced shift is -41.15, 93.48 unbinned pixels, unfiltered CCC is 0.0034 The average drift between shots was -5.1, 3.5 unbinned pixels, 0.17 nm/sec Measured defocus = -1.85 microns changed by 0.25 to target Tilt-induced shift is -35.93, 81.06 unbinned pixels, unfiltered CCC is 0.0030 The average drift between shots was -4.1, 2.6 unbinned pixels, 0.13 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0598_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0599_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0600_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0601_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0602_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0603_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0604_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0605_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0606_X+1Y+1-0.tif 313.266 Running script 3 at item 120-69 (-496.43, -385.58) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 118.15, -258.81 unbinned pixels, unfiltered CCC is 0.0357 The average drift between shots was -16.4, -6.6 unbinned pixels, 0.47 nm/sec Measured defocus = 5.14 microns changed by -6.94 to target Tilt-induced shift is -30.04, 71.15 unbinned pixels, unfiltered CCC is 0.0531 The average drift between shots was -8.2, 4.4 unbinned pixels, 0.26 nm/sec Measured defocus = -1.40 microns changed by -0.40 to target Tilt-induced shift is -38.84, 91.07 unbinned pixels, unfiltered CCC is 0.0517 The average drift between shots was -5.1, 2.9 unbinned pixels, 0.17 nm/sec Measured defocus = -1.80 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0607_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0608_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0609_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0610_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0611_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0612_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0613_X+1Y+1-0.tif 248.25 Running script 3 at item 120-73 (-490.49, -382.93) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 111.46, -255.50 unbinned pixels, unfiltered CCC is 0.0221 The average drift between shots was -12.1, 5.2 unbinned pixels, 0.37 nm/sec Measured defocus = 5.04 microns changed by -7.04 to target Tilt-induced shift is -28.01, 66.24 unbinned pixels, unfiltered CCC is 0.0977 The average drift between shots was -7.8, 4.4 unbinned pixels, 0.25 nm/sec Measured defocus = -1.31 microns changed by -0.69 to target Tilt-induced shift is -42.23, 97.94 unbinned pixels, unfiltered CCC is 0.0817 The average drift between shots was -4.2, -0.1 unbinned pixels, 0.11 nm/sec Measured defocus = -1.94 microns changed by -0.06 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0614_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0615_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0616_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0617_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0618_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0619_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0620_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0621_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0622_X+1Y+1-0.tif 310.828 Running script 3 at item 120-76 (-483.81, -382.50) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 23.53, -51.57 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -8.7, -2.6 unbinned pixels, 0.25 nm/sec Measured defocus = 1.03 microns changed by -3.23 to target Tilt-induced shift is -40.65, 95.41 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -5.0, 1.4 unbinned pixels, 0.14 nm/sec Measured defocus = -1.88 microns changed by -0.32 to target Tilt-induced shift is -47.06, 109.37 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -3.1, 1.3 unbinned pixels, 0.10 nm/sec Measured defocus = -2.16 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0623_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0624_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0625_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0626_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0627_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0628_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0629_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0630_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0631_X+1Y+1-0.tif 309.578 Running script 3 at item 120-91 (-470.45, -379.42) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 192.816119 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -44.71, 102.68 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -6.6, 2.0 unbinned pixels, 0.19 nm/sec Measured defocus = -2.03 microns changed by 1.03 to target Tilt-induced shift is -26.71, 59.11 unbinned pixels, unfiltered CCC is 0.0025 The average drift between shots was -5.0, 0.6 unbinned pixels, 0.14 nm/sec Measured defocus = -1.18 microns changed by 0.18 to target Tilt-induced shift is -22.64, 51.53 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -2.9, 0.7 unbinned pixels, 0.08 nm/sec Measured defocus = -1.02 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0632_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0633_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0634_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0635_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0636_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0637_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0638_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0639_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0640_X+1Y+1-0.tif 301.953 Running script 3 at item 120-94 (-477.06, -379.75) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 384.198874 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 15.58, -36.60 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -9.9, 4.0 unbinned pixels, 0.30 nm/sec Measured defocus = 0.72 microns changed by -1.92 to target Tilt-induced shift is -23.55, 55.78 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -6.9, 4.9 unbinned pixels, 0.23 nm/sec Measured defocus = -1.10 microns changed by -0.10 to target Tilt-induced shift is -27.08, 62.03 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -4.3, 3.6 unbinned pixels, 0.16 nm/sec Measured defocus = -1.23 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0641_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0642_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0643_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0644_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0645_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0646_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0647_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0648_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0649_X+1Y+1-0.tif 316.89 Running script 3 at item 120-106 (-497.08, -378.64) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 139.61, -309.98 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -13.2, -5.5 unbinned pixels, 0.40 nm/sec Measured defocus = 6.14 microns changed by -7.54 to target Tilt-induced shift is -18.07, 43.75 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -8.0, 3.9 unbinned pixels, 0.25 nm/sec Measured defocus = -0.86 microns changed by -0.54 to target Tilt-induced shift is -29.90, 68.96 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -4.3, 2.2 unbinned pixels, 0.14 nm/sec Measured defocus = -1.36 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0650_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0651_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0652_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0653_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0654_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0655_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0656_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0657_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0658_X+1Y+1-0.tif 306.828 Running script 3 at item 120-121 (-466.49, -376.90) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 175.16198 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -126.75, 296.39 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -7.1, -4.6 unbinned pixels, 0.23 nm/sec Measured defocus = -5.87 microns changed by 4.27 to target Tilt-induced shift is -42.74, 95.37 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -4.9, 2.8 unbinned pixels, 0.15 nm/sec Measured defocus = -1.90 microns changed by 0.30 to target Tilt-induced shift is -35.04, 81.68 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -2.7, 3.2 unbinned pixels, 0.12 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0659_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0660_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0661_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0662_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0663_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0664_X+1Y+1-0.tif 227.875 Running script 3 at item 120-131 (-484.24, -375.52) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 286.524646 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 20.92, -42.96 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -11.9, -1.4 unbinned pixels, 0.32 nm/sec Measured defocus = 0.87 microns changed by -2.67 to target Tilt-induced shift is -36.34, 84.32 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -5.5, 4.5 unbinned pixels, 0.19 nm/sec Measured defocus = -1.67 microns changed by -0.13 to target Tilt-induced shift is -39.35, 91.43 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -3.8, 3.0 unbinned pixels, 0.13 nm/sec Measured defocus = -1.81 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0665_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0666_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0667_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0668_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0669_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0670_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0671_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0672_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0673_X+1Y+1-0.tif 313.516 Running script 3 at item 120-134 (-490.95, -375.93) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 110.82, -238.90 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -14.5, -9.7 unbinned pixels, 0.48 nm/sec Measured defocus = 4.76 microns changed by -6.76 to target Tilt-induced shift is -29.79, 70.88 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -5.7, 7.8 unbinned pixels, 0.27 nm/sec Measured defocus = -1.40 microns changed by -0.60 to target Tilt-induced shift is -41.66, 97.51 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -4.2, 4.6 unbinned pixels, 0.18 nm/sec Measured defocus = -1.93 microns changed by -0.07 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0674_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0675_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0676_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0677_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0678_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0679_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0680_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0681_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0682_X+1Y+1-0.tif 21:27:10 Refine ZLP: additional offset = 0.8 eV; total offset = 4.2 eV 336.281 Running script 3 at item 120-149 (-477.39, -372.72) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 410.148886 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -28.47, 62.49 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -6.6, -0.0 unbinned pixels, 0.19 nm/sec Measured defocus = -1.25 microns changed by -0.95 to target Tilt-induced shift is -45.08, 105.33 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.9, 1.9 unbinned pixels, 0.19 nm/sec Measured defocus = -2.08 microns changed by -0.12 to target Tilt-induced shift is -48.33, 110.88 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -3.9, 0.8 unbinned pixels, 0.12 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0683_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0684_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0685_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0686_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0687_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0688_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0689_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0690_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0691_X+1Y+1-0.tif 305.968 Running script 3 at item 120-152 (-470.99, -372.42) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 461.95612 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -50.98, 119.97 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -8.4, 2.7 unbinned pixels, 0.25 nm/sec Measured defocus = -2.37 microns changed by 1.37 to target Tilt-induced shift is -23.80, 56.27 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -6.0, 4.1 unbinned pixels, 0.20 nm/sec Measured defocus = -1.11 microns changed by 0.11 to target Tilt-induced shift is -22.89, 51.00 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -3.5, 2.0 unbinned pixels, 0.11 nm/sec Measured defocus = -1.01 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0692_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0693_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0694_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0695_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0696_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0697_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0698_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0699_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0700_X+1Y+1-0.tif 308.641 Running script 3 at item 120-157 (-466.98, -369.87) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 426.780679 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -119.04, 291.73 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was -10.4, -19.1 unbinned pixels, 0.58 nm/sec Measured defocus = -5.74 microns changed by 4.54 to target Tilt-induced shift is -31.92, 73.05 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -6.5, 2.2 unbinned pixels, 0.19 nm/sec Measured defocus = -1.45 microns changed by 0.25 to target Tilt-induced shift is -26.01, 61.75 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -4.1, 3.0 unbinned pixels, 0.14 nm/sec Measured defocus = -1.22 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0701_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0702_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0703_X+1Y+0-0.tif 146.5 Running script 3 at item 120-170 (-497.46, -371.62) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 489.715752 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 175.54, -407.55 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -8.0, 3.2 unbinned pixels, 0.25 nm/sec Measured defocus = 8.01 microns changed by -9.41 to target Tilt-induced shift is -24.24, 54.36 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -6.0, 1.2 unbinned pixels, 0.17 nm/sec Measured defocus = -1.08 microns changed by -0.32 to target Tilt-induced shift is -30.03, 69.96 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -3.8, 2.3 unbinned pixels, 0.13 nm/sec Measured defocus = -1.38 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0704_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0705_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0706_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0707_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0708_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0709_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0710_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0711_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0712_X+1Y+1-0.tif 300.719 Running script 3 at item 120-176 (-491.16, -368.89) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 423.133379 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 79.05, -164.30 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -18.3, -10.0 unbinned pixels, 0.57 nm/sec Measured defocus = 3.30 microns changed by -4.90 to target Tilt-induced shift is -24.37, 56.94 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -5.9, 2.8 unbinned pixels, 0.18 nm/sec Measured defocus = -1.12 microns changed by -0.48 to target Tilt-induced shift is -34.26, 78.68 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -3.4, 3.0 unbinned pixels, 0.13 nm/sec Measured defocus = -1.56 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0713_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0714_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0715_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0716_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0717_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0718_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0719_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0720_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0721_X+1Y+1-0.tif 312.672 Running script 3 at item 120-179 (-484.51, -368.50) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 291.742384 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 39.23, -95.22 unbinned pixels, unfiltered CCC is 0.0025 The average drift between shots was -8.9, 0.9 unbinned pixels, 0.25 nm/sec Measured defocus = 1.87 microns changed by -3.67 to target Tilt-induced shift is -32.18, 73.71 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -5.7, 2.6 unbinned pixels, 0.18 nm/sec Measured defocus = -1.46 microns changed by -0.34 to target Tilt-induced shift is -38.89, 90.03 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -3.1, 0.2 unbinned pixels, 0.08 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0722_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0723_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0724_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0725_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0726_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0727_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0728_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0729_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0730_X+1Y+1-0.tif 316.516 Running script 3 at item 120-190 (-471.46, -365.39) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 209.281137 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -74.84, 177.47 unbinned pixels, unfiltered CCC is 0.0039 The average drift between shots was -10.5, -7.1 unbinned pixels, 0.35 nm/sec Measured defocus = -3.50 microns changed by 1.50 to target Tilt-induced shift is -45.37, 101.32 unbinned pixels, unfiltered CCC is 0.0067 The average drift between shots was -6.9, 5.7 unbinned pixels, 0.25 nm/sec Measured defocus = -2.02 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0731_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0732_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0733_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0734_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0735_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0736_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0737_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0738_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0739_X+1Y+1-0.tif 300.984 Running script 3 at item 120-193 (-477.80, -365.71) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 427.280962 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -2.92, 11.64 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -11.1, -0.7 unbinned pixels, 0.31 nm/sec Measured defocus = -0.21 microns changed by -1.99 to target Tilt-induced shift is -47.00, 106.78 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -5.0, 1.6 unbinned pixels, 0.15 nm/sec Measured defocus = -2.12 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0740_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0741_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0742_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0743_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0744_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0745_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0746_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0747_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0748_X+1Y+1-0.tif 297.515 Running script 3 at item 120-205 (-497.78, -366.97) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 242.005912 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 155.65, -355.33 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -7.7, -8.8 unbinned pixels, 0.32 nm/sec Measured defocus = 7.00 microns changed by -8.00 to target Tilt-induced shift is -11.24, 26.95 unbinned pixels, unfiltered CCC is 0.0034 The average drift between shots was -5.3, 2.3 unbinned pixels, 0.16 nm/sec Measured defocus = -0.53 microns changed by -0.47 to target Tilt-induced shift is -20.98, 48.11 unbinned pixels, unfiltered CCC is 0.0034 The average drift between shots was -3.4, 1.3 unbinned pixels, 0.10 nm/sec Measured defocus = -0.95 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0749_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0750_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0751_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0752_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0753_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0754_X+1Y+1-0.tif 224.203 Running script 3 at item 120-226 (-484.91, -361.48) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 224.293339 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 24.92, -51.36 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -9.0, 6.3 unbinned pixels, 0.30 nm/sec Measured defocus = 1.04 microns changed by -2.24 to target Tilt-induced shift is -21.22, 47.01 unbinned pixels, unfiltered CCC is 0.0031 The average drift between shots was -5.0, 10.9 unbinned pixels, 0.31 nm/sec Measured defocus = -0.94 microns changed by -0.26 to target Tilt-induced shift is -24.18, 57.09 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -4.0, 6.9 unbinned pixels, 0.22 nm/sec Measured defocus = -1.13 microns changed by -0.07 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0755_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0756_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0757_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0758_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0759_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0760_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0761_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0762_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0763_X+1Y+1-0.tif 308.188 Running script 3 at item 120-229 (-491.27, -364.20) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 92.59, -218.05 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -17.0, 7.6 unbinned pixels, 0.51 nm/sec Measured defocus = 4.28 microns changed by -5.68 to target Tilt-induced shift is -23.91, 56.47 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -8.4, 1.9 unbinned pixels, 0.24 nm/sec Measured defocus = -1.11 microns changed by -0.29 to target Tilt-induced shift is -30.12, 69.21 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.9, 0.4 unbinned pixels, 0.16 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0764_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0765_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0766_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0767_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0768_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0769_X+1Y+1-0.tif 231.063 Running script 3 at item 120-241 (-478.40, -358.75) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 327.090316 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -32.08, 79.45 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -7.9, -5.4 unbinned pixels, 0.26 nm/sec Measured defocus = -1.56 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0770_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0771_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0772_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0773_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0774_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0775_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0776_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0777_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0778_X+1Y+1-0.tif 296.265 Running script 3 at item 120-244 (-471.94, -358.36) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 311.587207 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -75.29, 175.29 unbinned pixels, unfiltered CCC is 0.0153 The average drift between shots was -9.0, -1.5 unbinned pixels, 0.26 nm/sec Measured defocus = -3.46 microns changed by 1.66 to target Tilt-induced shift is -43.80, 102.61 unbinned pixels, unfiltered CCC is 0.0178 The average drift between shots was -5.8, -0.0 unbinned pixels, 0.16 nm/sec Measured defocus = -2.03 microns changed by 0.23 to target Tilt-induced shift is -40.98, 93.88 unbinned pixels, unfiltered CCC is 0.0167 The average drift between shots was -3.2, -0.4 unbinned pixels, 0.09 nm/sec Measured defocus = -1.86 microns changed by 0.06 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0779_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0780_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0781_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0782_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0783_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0784_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0785_X-1Y+1-0.tif 22:28:43 Refine ZLP: additional offset = 0.9 eV; total offset = 5.1 eV 278.797 Running script 3 at item 121-1 (-160.14, -535.70) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 264.063473 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -21.09, 44.27 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -0.1, 7.9 unbinned pixels, 0.22 nm/sec Measured defocus = -0.89 microns changed by -1.11 to target Tilt-induced shift is -43.28, 100.38 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -2.9, 5.7 unbinned pixels, 0.18 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0786_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0787_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0788_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0789_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0790_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0791_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0792_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0793_X+1Y+1-0.tif Beam was shifted 0.021 microns 315.922 Running script 3 at item 121-3 (-153.08, -535.20) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 287.072168 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -14.28, 32.59 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -8.0, -0.6 unbinned pixels, 0.21 nm/sec Measured defocus = -0.65 microns changed by -1.55 to target Tilt-induced shift is -45.93, 106.55 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -5.3, -0.8 unbinned pixels, 0.15 nm/sec Measured defocus = -2.11 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0794_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0795_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0796_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0797_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0798_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0799_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0800_X+1Y+1-0.tif 248.219 Running script 3 at item 121-4 (-148.49, -532.54) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 243.171848 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -20.91, 42.66 unbinned pixels, unfiltered CCC is 0.0069 The average drift between shots was -6.5, 4.4 unbinned pixels, 0.22 nm/sec Measured defocus = -0.86 microns changed by -0.14 to target Tilt-induced shift is -21.97, 51.29 unbinned pixels, unfiltered CCC is 0.0060 The average drift between shots was -5.9, 0.9 unbinned pixels, 0.16 nm/sec Measured defocus = -1.01 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0801_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0802_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0803_X+0Y+1-0.tif 136.078 Running script 3 at item 121-11 (-167.36, -533.88) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 322.463957 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -9.24, 21.67 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -9.3, 2.3 unbinned pixels, 0.26 nm/sec Measured defocus = -0.43 microns changed by -0.77 to target Tilt-induced shift is -25.55, 58.87 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -5.0, 1.1 unbinned pixels, 0.14 nm/sec Measured defocus = -1.17 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0804_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0805_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0806_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0807_X+0Y+1-0.tif 171.719 Running script 3 at item 121-23 (-146.39, -527.68) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 169.584028 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -28.73, 65.87 unbinned pixels, unfiltered CCC is 0.0035 The average drift between shots was -10.1, 1.8 unbinned pixels, 0.29 nm/sec Measured defocus = -1.30 microns changed by -0.10 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0808_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0809_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0810_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0811_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0812_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0813_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0814_X-1Y+1-0.tif 245.203 Running script 3 at item 121-26 (-153.47, -528.19) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 284.988683 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -25.43, 57.43 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -7.4, 3.0 unbinned pixels, 0.22 nm/sec Measured defocus = -1.14 microns changed by -0.46 to target Tilt-induced shift is -35.14, 80.42 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -5.8, 0.7 unbinned pixels, 0.15 nm/sec Measured defocus = -1.59 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0815_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0816_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0817_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0818_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0819_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0820_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0821_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0822_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0823_X+1Y+1-0.tif 311.594 Running script 3 at item 121-29 (-160.54, -528.71) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 216.062211 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -35.88, 84.93 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -8.9, 0.9 unbinned pixels, 0.24 nm/sec Measured defocus = -1.67 microns changed by -0.13 to target Tilt-induced shift is -39.52, 90.54 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -5.7, 0.7 unbinned pixels, 0.15 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0824_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0825_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0826_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0827_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0828_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0829_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0830_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0831_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0832_X+1Y+1-0.tif 315.438 Running script 3 at item 121-32 (-167.63, -529.22) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 483.009596 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -29.57, 63.24 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -7.5, 4.9 unbinned pixels, 0.24 nm/sec Measured defocus = -1.27 microns changed by -0.73 to target Tilt-induced shift is -42.97, 99.98 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -7.0, 0.4 unbinned pixels, 0.19 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0833_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0834_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0835_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0836_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0837_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0838_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0839_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0840_X+1Y+1-0.tif 277.156 Running script 3 at item 121-35 (-174.85, -527.46) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 272.380026 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -26.59, 62.74 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -9.2, 3.4 unbinned pixels, 0.26 nm/sec Measured defocus = -1.24 microns changed by -0.96 to target Tilt-induced shift is -46.02, 107.91 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -6.4, 2.3 unbinned pixels, 0.18 nm/sec Measured defocus = -2.13 microns changed by -0.07 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0841_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0842_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0843_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0844_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0845_X+0Y+1-0.tif 192.734 Running script 3 at item 121-61 (-177.48, -522.99) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 201.114166 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -49.14, 112.86 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -3.6, -1.0 unbinned pixels, 0.10 nm/sec Measured defocus = -2.24 microns changed by 1.24 to target Tilt-induced shift is -23.44, 55.87 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -4.4, 1.0 unbinned pixels, 0.12 nm/sec Measured defocus = -1.10 microns changed by 0.10 to target Tilt-induced shift is -22.35, 50.57 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -4.1, 0.2 unbinned pixels, 0.11 nm/sec Measured defocus = -1.00 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0846_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0847_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0848_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0849_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0850_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0851_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0852_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0853_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0854_X+1Y+1-0.tif 300.64 Running script 3 at item 121-64 (-170.36, -522.42) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 353.306946 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -42.31, 105.08 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -10.9, -2.7 unbinned pixels, 0.31 nm/sec Measured defocus = -2.06 microns changed by 0.86 to target Tilt-induced shift is -28.29, 62.62 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.9, 2.2 unbinned pixels, 0.16 nm/sec Measured defocus = -1.25 microns changed by 0.05 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0855_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0856_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0857_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0858_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0859_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0860_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0861_X+0Y+1-0.tif 249.062 Running script 3 at item 121-66 (-163.29, -521.87) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 270.72124 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -37.04, 88.11 unbinned pixels, unfiltered CCC is 0.0106 The average drift between shots was -9.6, 1.3 unbinned pixels, 0.26 nm/sec Measured defocus = -1.74 microns changed by 0.34 to target Tilt-induced shift is -31.18, 75.45 unbinned pixels, unfiltered CCC is 0.0100 The average drift between shots was -7.5, -0.2 unbinned pixels, 0.20 nm/sec Measured defocus = -1.48 microns changed by 0.08 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0862_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0863_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0864_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0865_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0866_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0867_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0868_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0869_X+1Y+1-0.tif 282.079 Running script 3 at item 121-68 (-156.23, -521.36) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 188.583767 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -52.96, 121.62 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -7.0, -1.5 unbinned pixels, 0.19 nm/sec Measured defocus = -2.41 microns changed by 0.81 to target Tilt-induced shift is -36.95, 83.79 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -3.8, 1.0 unbinned pixels, 0.11 nm/sec Measured defocus = -1.66 microns changed by 0.06 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0870_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0871_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0872_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0873_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0874_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0875_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0876_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0877_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0878_X+1Y+1-0.tif 311.703 Running script 3 at item 121-71 (-149.17, -520.84) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 410.059717 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -40.80, 97.60 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -9.8, 4.3 unbinned pixels, 0.30 nm/sec Measured defocus = -1.92 microns changed by 0.12 to target Tilt-induced shift is -40.23, 92.13 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -6.2, 2.3 unbinned pixels, 0.17 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0879_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0880_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0881_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0882_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0883_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0884_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0885_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0886_X+0Y+1-0.tif 23:30:57 Refine ZLP: additional offset = -0.3 eV; total offset = 4.8 eV 295.282 Running script 3 at item 121-102 (-151.92, -514.01) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 371.52913 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -74.00, 168.64 unbinned pixels, unfiltered CCC is 0.0114 The average drift between shots was -6.5, 2.8 unbinned pixels, 0.20 nm/sec Measured defocus = -3.34 microns changed by 1.34 to target Tilt-induced shift is -46.62, 109.37 unbinned pixels, unfiltered CCC is 0.0146 The average drift between shots was -5.5, 1.0 unbinned pixels, 0.15 nm/sec Measured defocus = -2.16 microns changed by 0.16 to target Tilt-induced shift is -43.71, 101.51 unbinned pixels, unfiltered CCC is 0.0159 The average drift between shots was -3.0, 1.4 unbinned pixels, 0.09 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0887_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0888_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0889_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0890_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0891_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0892_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0893_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0894_X+0Y+1-0.tif 272.719 Running script 3 at item 121-105 (-158.97, -514.53) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 274.926609 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -56.01, 129.85 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -8.0, 3.5 unbinned pixels, 0.24 nm/sec Measured defocus = -2.57 microns changed by 0.37 to target Tilt-induced shift is -49.23, 113.15 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -4.8, 2.3 unbinned pixels, 0.15 nm/sec Measured defocus = -2.24 microns changed by 0.04 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0895_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0896_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0897_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0898_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0899_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0900_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0901_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0902_X+1Y+1-0.tif 286.328 Running script 3 at item 121-107 (-166.03, -515.07) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 291.937538 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -74.79, 168.45 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -5.0, 1.1 unbinned pixels, 0.14 nm/sec Measured defocus = -3.35 microns changed by 2.35 to target Tilt-induced shift is -27.11, 63.19 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -3.2, 1.5 unbinned pixels, 0.10 nm/sec Measured defocus = -1.25 microns changed by 0.25 to target Tilt-induced shift is -22.43, 52.08 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -3.1, 0.6 unbinned pixels, 0.09 nm/sec Measured defocus = -1.03 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0903_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0904_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0905_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0906_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0907_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0908_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0909_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0910_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0911_X+1Y+1-0.tif 317.375 Running script 3 at item 121-110 (-173.10, -515.60) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 210.695566 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -60.35, 139.54 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -7.6, 1.9 unbinned pixels, 0.21 nm/sec Measured defocus = -2.76 microns changed by 1.56 to target Tilt-induced shift is -30.13, 69.40 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -6.2, 0.9 unbinned pixels, 0.17 nm/sec Measured defocus = -1.37 microns changed by 0.17 to target Tilt-induced shift is -26.69, 61.28 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -3.7, 1.3 unbinned pixels, 0.11 nm/sec Measured defocus = -1.21 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0912_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0913_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0914_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0915_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0916_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0917_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0918_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0919_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0920_X+1Y+1-0.tif 308.984 Running script 3 at item 121-113 (-180.27, -516.16) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 449.904096 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -58.92, 139.53 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -8.2, 3.3 unbinned pixels, 0.24 nm/sec Measured defocus = -2.75 microns changed by 1.35 to target Tilt-induced shift is -33.02, 76.09 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -5.1, 0.6 unbinned pixels, 0.14 nm/sec Measured defocus = -1.51 microns changed by 0.11 to target Tilt-induced shift is -31.40, 71.35 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -3.8, 0.4 unbinned pixels, 0.10 nm/sec Measured defocus = -1.42 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0921_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0922_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0923_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0924_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0925_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0926_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0927_X+1Y+1-0.tif 245.672 Running script 3 at item 121-143 (-180.65, -509.15) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 385.657326 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -72.78, 167.81 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -9.1, 2.8 unbinned pixels, 0.26 nm/sec Measured defocus = -3.32 microns changed by 1.72 to target Tilt-induced shift is -39.59, 90.57 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.9, 1.5 unbinned pixels, 0.14 nm/sec Measured defocus = -1.79 microns changed by 0.19 to target Tilt-induced shift is -34.19, 82.03 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -1.6, 2.1 unbinned pixels, 0.07 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0928_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0929_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0930_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0931_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0932_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0933_X-1Y+0-0.tif 220.265 Running script 3 at item 121-145 (-175.88, -508.77) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 422.398068 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -78.27, 179.97 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -7.4, 1.7 unbinned pixels, 0.20 nm/sec Measured defocus = -3.56 microns changed by 1.76 to target Tilt-induced shift is -44.74, 101.42 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -4.9, 0.8 unbinned pixels, 0.13 nm/sec Measured defocus = -2.01 microns changed by 0.21 to target Tilt-induced shift is -40.59, 92.34 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -2.8, 0.9 unbinned pixels, 0.08 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0934_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0935_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0936_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0937_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0938_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0939_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0940_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0941_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0942_X+1Y+1-0.tif 353.969 Running script 3 at item 121-148 (-168.76, -508.22) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 293.014373 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -74.48, 167.97 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -7.4, 1.9 unbinned pixels, 0.21 nm/sec Measured defocus = -3.34 microns changed by 1.34 to target Tilt-induced shift is -46.76, 109.67 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was -5.0, 0.6 unbinned pixels, 0.14 nm/sec Measured defocus = -2.16 microns changed by 0.16 to target Tilt-induced shift is -44.29, 102.32 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -4.1, 0.9 unbinned pixels, 0.11 nm/sec Measured defocus = -2.02 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0943_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0944_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0945_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0946_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0947_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0948_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0949_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0950_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0951_X+1Y+1-0.tif 563.313 Running script 3 at item 121-151 (-161.71, -507.71) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 357.370503 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -85.15, 202.66 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -9.3, 1.0 unbinned pixels, 0.25 nm/sec Measured defocus = -3.99 microns changed by 1.79 to target Tilt-induced shift is -52.12, 121.89 unbinned pixels, unfiltered CCC is 0.0096 The average drift between shots was -4.6, 2.9 unbinned pixels, 0.15 nm/sec Measured defocus = -2.41 microns changed by 0.21 to target Tilt-induced shift is -48.32, 111.25 unbinned pixels, unfiltered CCC is 0.0102 The average drift between shots was -3.1, 1.0 unbinned pixels, 0.09 nm/sec Measured defocus = -2.20 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0952_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0953_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0954_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0955_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0956_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0957_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0958_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0959_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0960_X+1Y+1-0.tif 724.219 Running script 3 at item 121-154 (-154.67, -507.17) Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 453.80524 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -75.31, 170.99 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -8.0, 5.7 unbinned pixels, 0.27 nm/sec Measured defocus = -3.39 microns changed by 2.39 to target Tilt-induced shift is -27.72, 64.74 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -4.5, 2.9 unbinned pixels, 0.15 nm/sec Measured defocus = -1.28 microns changed by 0.28 to target Tilt-induced shift is -22.50, 52.35 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -2.8, 2.2 unbinned pixels, 0.10 nm/sec Measured defocus = -1.03 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0961_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0962_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0963_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0964_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0965_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0966_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0967_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0968_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0969_X+1Y+1-0.tif 00:35:42 Refine ZLP: additional offset = 0.7 eV; total offset = 5.5 eV 328.016 Running script 3 at item 121-185 (-157.40, -500.32) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 351.521839 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -99.09, 226.60 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -4.7, 6.0 unbinned pixels, 0.21 nm/sec Measured defocus = -4.49 microns changed by 3.29 to target Tilt-induced shift is -34.83, 79.09 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -4.6, 1.7 unbinned pixels, 0.14 nm/sec Measured defocus = -1.57 microns changed by 0.37 to target Tilt-induced shift is -27.47, 61.85 unbinned pixels, unfiltered CCC is 0.0029 The average drift between shots was -3.1, 2.5 unbinned pixels, 0.11 nm/sec Measured defocus = -1.23 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0970_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0971_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0972_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0973_X-1Y+1-0.tif 174.172 Running script 3 at item 121-187 (-162.09, -500.67) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 153.689466 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -90.63, 212.09 unbinned pixels, unfiltered CCC is 0.0741 The average drift between shots was -4.8, 0.2 unbinned pixels, 0.13 nm/sec Measured defocus = -4.19 microns changed by 2.79 to target Tilt-induced shift is -39.86, 88.23 unbinned pixels, unfiltered CCC is 0.0835 The average drift between shots was -2.1, 1.7 unbinned pixels, 0.07 nm/sec Measured defocus = -1.76 microns changed by 0.36 to target Tilt-induced shift is -30.47, 71.68 unbinned pixels, unfiltered CCC is 0.0829 The average drift between shots was -2.9, 0.7 unbinned pixels, 0.08 nm/sec Measured defocus = -1.41 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0974_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0975_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0976_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0977_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0978_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0979_X+1Y+1-0.tif 219.172 Running script 3 at item 121-189 (-169.02, -503.53) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 252.608287 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -89.67, 210.43 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -8.0, 2.5 unbinned pixels, 0.23 nm/sec Measured defocus = -4.15 microns changed by 2.55 to target Tilt-induced shift is -41.25, 94.08 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.9, 1.8 unbinned pixels, 0.17 nm/sec Measured defocus = -1.87 microns changed by 0.27 to target Tilt-induced shift is -35.60, 82.57 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -3.5, 1.0 unbinned pixels, 0.10 nm/sec Measured defocus = -1.63 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0980_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0981_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0982_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0983_X+1Y+1-0.tif 171.468 Running script 3 at item 121-192 (-173.76, -503.89) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 274.195142 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -89.65, 200.95 unbinned pixels, unfiltered CCC is 0.2324 The average drift between shots was -6.9, 3.2 unbinned pixels, 0.21 nm/sec Measured defocus = -4.00 microns changed by 2.20 to target Tilt-induced shift is -44.17, 100.67 unbinned pixels, unfiltered CCC is 0.2371 The average drift between shots was -9.1, -1.2 unbinned pixels, 0.25 nm/sec Measured defocus = -2.00 microns changed by 0.20 to target Tilt-induced shift is -38.97, 91.23 unbinned pixels, unfiltered CCC is 0.2429 The average drift between shots was -6.0, -1.2 unbinned pixels, 0.17 nm/sec Measured defocus = -1.80 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 2 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0984_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0985_X+0Y+0-0.tif 111.328 Running script 3 at item 122-1 (-489.58, 490.90) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 18.86, -45.39 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -1.2, 4.1 unbinned pixels, 0.12 nm/sec Measured defocus = 0.89 microns changed by -2.89 to target Tilt-induced shift is -42.39, 96.78 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -0.8, 3.4 unbinned pixels, 0.10 nm/sec Measured defocus = -1.92 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0986_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0987_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0988_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0989_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0990_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0991_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0992_X+1Y+1-0.tif Beam was shifted 0.009 microns 284.859 Running script 3 at item 122-3 (-482.59, 491.54) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 420.09972 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -0.17, 1.34 unbinned pixels, unfiltered CCC is 0.0864 The average drift between shots was -10.0, 9.1 unbinned pixels, 0.37 nm/sec Measured defocus = -0.02 microns changed by -2.18 to target Tilt-induced shift is -45.48, 105.01 unbinned pixels, unfiltered CCC is 0.0633 The average drift between shots was -5.3, 3.5 unbinned pixels, 0.17 nm/sec Measured defocus = -2.08 microns changed by -0.12 to target Tilt-induced shift is -48.34, 110.85 unbinned pixels, unfiltered CCC is 0.0630 The average drift between shots was -4.4, 3.5 unbinned pixels, 0.16 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0993_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0994_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0995_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0996_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0997_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0998_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_0999_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1000_X+1Y+1-0.tif 289.594 Running script 3 at item 122-6 (-475.84, 494.53) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -37.43, 89.69 unbinned pixels, unfiltered CCC is 0.1274 The average drift between shots was -12.6, 2.5 unbinned pixels, 0.36 nm/sec Measured defocus = -1.76 microns changed by 0.76 to target Tilt-induced shift is -22.15, 52.42 unbinned pixels, unfiltered CCC is 0.1305 The average drift between shots was -7.6, 4.4 unbinned pixels, 0.24 nm/sec Measured defocus = -1.03 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1001_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1002_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1003_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1004_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1005_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1006_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1007_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1008_X+1Y+1-0.tif 287.203 Running script 3 at item 122-22 (-469.06, 497.50) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 393.002885 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -23.34, 56.57 unbinned pixels, unfiltered CCC is 0.1442 The average drift between shots was -9.6, 4.6 unbinned pixels, 0.29 nm/sec Measured defocus = -1.11 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1009_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1010_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1011_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1012_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1013_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1014_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1015_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1016_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1017_X+1Y+1-0.tif 307.953 Running script 3 at item 122-25 (-462.29, 500.45) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 199.166595 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -58.04, 138.09 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -10.0, 1.8 unbinned pixels, 0.27 nm/sec Measured defocus = -2.72 microns changed by 1.32 to target Tilt-induced shift is -33.88, 77.17 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -6.0, 3.0 unbinned pixels, 0.19 nm/sec Measured defocus = -1.53 microns changed by 0.13 to target Tilt-induced shift is -31.12, 71.63 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -3.6, 2.8 unbinned pixels, 0.13 nm/sec Measured defocus = -1.42 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1018_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1019_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1020_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1021_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1022_X+0Y+1-0.tif 205.5 Running script 3 at item 122-32 (-483.24, 498.54) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 254.835587 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 8.19, -17.94 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -12.3, 2.2 unbinned pixels, 0.33 nm/sec Measured defocus = 0.36 microns changed by -1.96 to target Tilt-induced shift is -33.46, 77.66 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -5.0, 3.3 unbinned pixels, 0.16 nm/sec Measured defocus = -1.54 microns changed by -0.06 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1023_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1024_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1025_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1026_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1027_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1028_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1029_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1030_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1031_X+1Y+1-0.tif 314.109 Running script 3 at item 122-35 (-490.25, 497.91) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 191.418644 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 4.85, -9.56 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -6.9, -3.3 unbinned pixels, 0.21 nm/sec Measured defocus = 0.19 microns changed by -1.99 to target Tilt-induced shift is -34.17, 78.70 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.8, 2.2 unbinned pixels, 0.17 nm/sec Measured defocus = -1.56 microns changed by -0.24 to target Tilt-induced shift is -39.07, 90.65 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -4.5, 2.8 unbinned pixels, 0.15 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1032_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1033_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1034_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1035_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1036_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1037_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1038_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1039_X+1Y+1-0.tif 279.922 Running script 3 at item 122-43 (-476.51, 501.53) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 329.947127 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -30.48, 71.92 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -8.2, -3.0 unbinned pixels, 0.24 nm/sec Measured defocus = -1.42 microns changed by -0.58 to target Tilt-induced shift is -43.04, 98.07 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -4.9, 3.0 unbinned pixels, 0.16 nm/sec Measured defocus = -1.94 microns changed by -0.06 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1040_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1041_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1042_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1043_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1044_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1045_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1046_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1047_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1048_X+1Y+1-0.tif 311.922 Running script 3 at item 122-52 (-458.09, 505.51) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 274.44353 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -52.04, 121.88 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -9.6, 3.5 unbinned pixels, 0.28 nm/sec Measured defocus = -2.41 microns changed by 0.21 to target Tilt-induced shift is -47.60, 112.52 unbinned pixels, unfiltered CCC is -0.0015 The average drift between shots was -5.7, 3.1 unbinned pixels, 0.18 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1049_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1050_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1051_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1052_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1053_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1054_X+1Y+1-0.tif 227.531 Running script 3 at item 122-54 (-462.74, 505.11) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 421.633873 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -49.46, 119.62 unbinned pixels, unfiltered CCC is 0.0204 The average drift between shots was -11.9, 3.9 unbinned pixels, 0.35 nm/sec Measured defocus = -2.35 microns changed by 1.35 to target Tilt-induced shift is -27.42, 60.61 unbinned pixels, unfiltered CCC is 0.0217 The average drift between shots was -4.9, 1.6 unbinned pixels, 0.13 nm/sec Measured defocus = -1.21 microns changed by 0.21 to target Tilt-induced shift is -22.98, 50.50 unbinned pixels, unfiltered CCC is 0.0199 The average drift between shots was -3.4, 0.5 unbinned pixels, 0.09 nm/sec Measured defocus = -1.01 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1055_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1056_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1057_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1058_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1059_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1060_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1061_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1062_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1063_X+1Y+1-0.tif 316.297 Running script 3 at item 122-57 (-469.73, 504.50) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 377.663149 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -24.54, 63.61 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -10.1, -1.7 unbinned pixels, 0.27 nm/sec Measured defocus = -1.24 microns changed by 0.04 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1064_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1065_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1066_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1067_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1068_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1069_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1070_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1071_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1072_X+1Y+1-0.tif 01:40:45 Refine ZLP: additional offset = 0.5 eV; total offset = 6.0 eV 318.547 Running script 3 at item 122-69 (-495.38, 502.14) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 14.97, -32.55 unbinned pixels, unfiltered CCC is 0.2629 The average drift between shots was -11.0, -1.8 unbinned pixels, 0.30 nm/sec Measured defocus = 0.65 microns changed by -2.05 to target Tilt-induced shift is -28.53, 63.54 unbinned pixels, unfiltered CCC is 0.2481 The average drift between shots was -6.8, 2.1 unbinned pixels, 0.20 nm/sec Measured defocus = -1.26 microns changed by -0.14 to target Tilt-induced shift is -30.66, 69.23 unbinned pixels, unfiltered CCC is 0.2532 The average drift between shots was -4.7, 2.7 unbinned pixels, 0.15 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1073_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1074_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1075_X+1Y+0-0.tif 151.171 Running script 3 at item 122-71 (-490.95, 504.90) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 243.732446 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 15.11, -32.19 unbinned pixels, unfiltered CCC is 0.1406 The average drift between shots was -10.7, 3.4 unbinned pixels, 0.32 nm/sec Measured defocus = 0.65 microns changed by -2.25 to target Tilt-induced shift is -32.45, 74.55 unbinned pixels, unfiltered CCC is 0.1204 The average drift between shots was -5.4, 0.5 unbinned pixels, 0.14 nm/sec Measured defocus = -1.48 microns changed by -0.12 to target Tilt-induced shift is -34.85, 79.86 unbinned pixels, unfiltered CCC is 0.1186 The average drift between shots was -3.1, 0.6 unbinned pixels, 0.09 nm/sec Measured defocus = -1.58 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1076_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1077_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1078_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1079_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1080_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1081_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1082_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1083_X+1Y+1-0.tif 288.359 Running script 3 at item 122-73 (-483.96, 505.55) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 447.997004 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -15.34, 38.75 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -7.5, -3.4 unbinned pixels, 0.22 nm/sec Measured defocus = -0.76 microns changed by -1.04 to target Tilt-induced shift is -36.28, 83.28 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.6, 1.7 unbinned pixels, 0.16 nm/sec Measured defocus = -1.65 microns changed by -0.15 to target Tilt-induced shift is -38.82, 90.44 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.0, 1.0 unbinned pixels, 0.09 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1084_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1085_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1086_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1087_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1088_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1089_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1090_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1091_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1092_X+1Y+1-0.tif 318.86 Running script 3 at item 122-95 (-477.17, 508.52) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 498.621991 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -11.14, 33.04 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -9.4, 4.3 unbinned pixels, 0.29 nm/sec Measured defocus = -0.63 microns changed by -1.37 to target Tilt-induced shift is -42.16, 96.84 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was -4.4, 0.5 unbinned pixels, 0.12 nm/sec Measured defocus = -1.92 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1093_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1094_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1095_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1096_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1097_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1098_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1099_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1100_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1101_X+1Y+1-0.tif 301.859 Running script 3 at item 122-105 (-496.09, 509.13) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 142.098177 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 16.49, -38.85 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -8.6, 0.0 unbinned pixels, 0.23 nm/sec Measured defocus = 0.77 microns changed by -2.97 to target Tilt-induced shift is -42.81, 99.52 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -5.6, 2.2 unbinned pixels, 0.17 nm/sec Measured defocus = -1.97 microns changed by -0.23 to target Tilt-induced shift is -47.59, 110.32 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -4.0, 1.8 unbinned pixels, 0.12 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1102_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1103_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1104_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1105_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1106_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1107_X-1Y+0-0.tif 208.125 Running script 3 at item 122-116 (-470.43, 511.49) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 475.560219 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -48.59, 114.82 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -6.4, -0.1 unbinned pixels, 0.17 nm/sec Measured defocus = -2.26 microns changed by 1.26 to target Tilt-induced shift is -23.99, 55.94 unbinned pixels, unfiltered CCC is 0.0025 The average drift between shots was -4.7, 1.3 unbinned pixels, 0.14 nm/sec Measured defocus = -1.11 microns changed by 0.11 to target Tilt-induced shift is -22.70, 51.22 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -2.3, 1.6 unbinned pixels, 0.08 nm/sec Measured defocus = -1.02 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1108_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1109_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1110_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1111_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1112_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1113_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1114_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1115_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1116_X+1Y+1-0.tif 300.984 Running script 3 at item 122-119 (-463.43, 512.11) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 365.575402 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -37.20, 86.07 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -9.1, 2.8 unbinned pixels, 0.26 nm/sec Measured defocus = -1.70 microns changed by 0.50 to target Tilt-induced shift is -26.92, 62.34 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -5.6, 3.5 unbinned pixels, 0.18 nm/sec Measured defocus = -1.23 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1117_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1118_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1119_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1120_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1121_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1122_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1123_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1124_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1125_X+1Y+1-0.tif 306.563 Running script 3 at item 122-122 (-458.76, 512.52) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 453.248983 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -62.49, 149.46 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -11.0, 2.8 unbinned pixels, 0.31 nm/sec Measured defocus = -2.94 microns changed by 1.54 to target Tilt-induced shift is -36.80, 80.01 unbinned pixels, unfiltered CCC is 0.0030 The average drift between shots was -4.6, 3.4 unbinned pixels, 0.15 nm/sec Measured defocus = -1.60 microns changed by 0.20 to target Tilt-induced shift is -31.39, 71.83 unbinned pixels, unfiltered CCC is 0.0030 The average drift between shots was -3.1, 1.8 unbinned pixels, 0.10 nm/sec Measured defocus = -1.42 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1126_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1127_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1128_X+1Y+0-0.tif 142.547 Running script 3 at item 122-133 (-484.66, 512.55) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 391.664222 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -6.93, 11.43 unbinned pixels, unfiltered CCC is 0.0342 The average drift between shots was -9.4, -2.0 unbinned pixels, 0.27 nm/sec Measured defocus = -0.24 microns changed by -1.36 to target Tilt-induced shift is -32.20, 75.77 unbinned pixels, unfiltered CCC is 0.0258 The average drift between shots was -5.4, 2.9 unbinned pixels, 0.16 nm/sec Measured defocus = -1.49 microns changed by -0.11 to target Tilt-induced shift is -35.49, 81.10 unbinned pixels, unfiltered CCC is 0.0266 The average drift between shots was -4.4, 2.6 unbinned pixels, 0.14 nm/sec Measured defocus = -1.61 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1129_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1130_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1131_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1132_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1133_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1134_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1135_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1136_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1137_X+1Y+1-0.tif 320.797 Running script 3 at item 122-136 (-491.62, 511.89) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 191.160238 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 19.23, -47.59 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -9.3, 2.7 unbinned pixels, 0.26 nm/sec Measured defocus = 0.93 microns changed by -2.73 to target Tilt-induced shift is -35.82, 81.47 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -5.6, 1.5 unbinned pixels, 0.16 nm/sec Measured defocus = -1.62 microns changed by -0.18 to target Tilt-induced shift is -38.24, 89.99 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.0, 0.9 unbinned pixels, 0.12 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1138_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1139_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1140_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1141_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1142_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1143_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1144_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1145_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1146_X+1Y+1-0.tif 317.687 Running script 3 at item 122-149 (-477.88, 515.53) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 332.015347 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -21.02, 47.21 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -6.7, -2.0 unbinned pixels, 0.19 nm/sec Measured defocus = -0.94 microns changed by -1.06 to target Tilt-induced shift is -40.41, 93.90 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -7.0, 3.9 unbinned pixels, 0.21 nm/sec Measured defocus = -1.86 microns changed by -0.14 to target Tilt-induced shift is -43.07, 100.10 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -2.8, 2.3 unbinned pixels, 0.10 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1147_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1148_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1149_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1150_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1151_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1152_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1153_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1154_X+1Y+1-0.tif 280.344 Running script 3 at item 122-159 (-464.10, 519.12) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 322.191287 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -54.46, 126.12 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -7.6, 2.4 unbinned pixels, 0.22 nm/sec Measured defocus = -2.49 microns changed by 0.29 to target Tilt-induced shift is -48.15, 112.35 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -3.9, 3.0 unbinned pixels, 0.13 nm/sec Measured defocus = -2.22 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1155_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1156_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1157_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1158_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1159_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1160_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1161_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1162_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1163_X+1Y+1-0.tif 304.797 Running script 3 at item 122-162 (-471.09, 518.50) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 218.651574 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -22.33, 54.32 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -8.9, 3.7 unbinned pixels, 0.27 nm/sec Measured defocus = -1.07 microns changed by 0.07 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1164_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1165_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1166_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1167_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1168_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1169_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1170_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1171_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1172_X+1Y+1-0.tif 02:41:07 Refine ZLP: additional offset = -0.3 eV; total offset = 5.7 eV 311.532 Running script 3 at item 122-174 (-499.12, 515.91) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 150.025784 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 28.08, -63.84 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -10.1, -0.0 unbinned pixels, 0.28 nm/sec Measured defocus = 1.27 microns changed by -2.47 to target Tilt-induced shift is -22.09, 52.15 unbinned pixels, unfiltered CCC is -0.0010 The average drift between shots was -5.2, 2.0 unbinned pixels, 0.15 nm/sec Measured defocus = -1.03 microns changed by -0.17 to target Tilt-induced shift is -27.08, 59.44 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -3.3, 3.1 unbinned pixels, 0.12 nm/sec Measured defocus = -1.19 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1173_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1174_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1175_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1176_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1177_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1178_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1179_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1180_X+1Y+1-0.tif 252.578 Running script 3 at item 122-180 (-492.34, 518.92) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 405.121777 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 0.54, -1.50 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -8.8, -0.8 unbinned pixels, 0.24 nm/sec Measured defocus = 0.03 microns changed by -1.43 to target Tilt-induced shift is -27.92, 65.15 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -5.9, 3.1 unbinned pixels, 0.18 nm/sec Measured defocus = -1.29 microns changed by -0.11 to target Tilt-induced shift is -30.04, 69.55 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -4.1, 2.7 unbinned pixels, 0.14 nm/sec Measured defocus = -1.38 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1181_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1182_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1183_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1184_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1185_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1186_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1187_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1188_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1189_X+1Y+1-0.tif 318.437 Running script 3 at item 122-183 (-485.32, 519.57) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 182.040587 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 7.31, -10.60 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -10.7, 2.7 unbinned pixels, 0.29 nm/sec Measured defocus = 0.23 microns changed by -1.83 to target Tilt-induced shift is -32.60, 75.30 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -4.1, 1.5 unbinned pixels, 0.12 nm/sec Measured defocus = -1.49 microns changed by -0.11 to target Tilt-induced shift is -34.72, 80.36 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -1.9, 0.4 unbinned pixels, 0.05 nm/sec Measured defocus = -1.59 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1190_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1191_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1192_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1193_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1194_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1195_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1196_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1197_X+1Y+1-0.tif 296.359 Running script 3 at item 122-199 (-478.59, 522.56) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 252.338349 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -27.14, 62.59 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -10.4, 4.1 unbinned pixels, 0.31 nm/sec Measured defocus = -1.24 microns changed by -0.56 to target Tilt-induced shift is -38.93, 89.94 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -5.4, 1.7 unbinned pixels, 0.15 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1198_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1199_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1200_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1201_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1202_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1203_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1204_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1205_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1206_X+1Y+1-0.tif 315.14 Running script 3 at item 122-210 (-499.82, 522.94) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 327.805641 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 34.51, -79.68 unbinned pixels, unfiltered CCC is 0.0176 The average drift between shots was -8.9, 0.9 unbinned pixels, 0.24 nm/sec Measured defocus = 1.58 microns changed by -3.58 to target Tilt-induced shift is -38.16, 91.62 unbinned pixels, unfiltered CCC is 0.0177 The average drift between shots was -6.6, 1.2 unbinned pixels, 0.18 nm/sec Measured defocus = -1.80 microns changed by -0.20 to target Tilt-induced shift is -43.74, 101.25 unbinned pixels, unfiltered CCC is 0.0175 The average drift between shots was -3.5, -0.0 unbinned pixels, 0.09 nm/sec Measured defocus = -2.00 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1207_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1208_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1209_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1210_X+0Y+1-0.tif 158.421 Running script 3 at item 122-221 (-471.75, 525.55) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 379.339148 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -32.71, 78.12 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -7.2, -1.5 unbinned pixels, 0.21 nm/sec Measured defocus = -1.54 microns changed by -0.66 to target Tilt-induced shift is -45.28, 107.03 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -4.9, 4.5 unbinned pixels, 0.18 nm/sec Measured defocus = -2.11 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1211_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1212_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1213_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1214_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1215_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1216_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1217_X+1Y+1-0.tif 246.204 Running script 3 at item 122-222 (-467.07, 525.96) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 433.019522 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -38.89, 91.85 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -9.6, 3.4 unbinned pixels, 0.27 nm/sec Measured defocus = -1.81 microns changed by 0.81 to target Tilt-induced shift is -24.81, 55.98 unbinned pixels, unfiltered CCC is -0.0011 The average drift between shots was -4.7, 1.4 unbinned pixels, 0.13 nm/sec Measured defocus = -1.11 microns changed by 0.11 to target Tilt-induced shift is -22.58, 51.42 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -2.9, 2.7 unbinned pixels, 0.11 nm/sec Measured defocus = -1.02 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1218_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1219_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1220_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1221_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1222_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1223_X+1Y+1-0.tif 205.922 Running script 3 at item 122-232 (-486.02, 526.60) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 171.413096 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 2.92, -11.53 unbinned pixels, unfiltered CCC is 0.0044 The average drift between shots was -8.7, -0.8 unbinned pixels, 0.24 nm/sec Measured defocus = 0.21 microns changed by -1.41 to target Tilt-induced shift is -24.87, 56.14 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -5.5, 3.6 unbinned pixels, 0.18 nm/sec Measured defocus = -1.11 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1224_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1225_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1226_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1227_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1228_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1229_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1230_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1231_X+1Y+1-0.tif 283.734 Running script 3 at item 122-235 (-492.82, 523.60) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 352.536551 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 4.11, -8.62 unbinned pixels, unfiltered CCC is 0.0034 The average drift between shots was -9.6, 1.1 unbinned pixels, 0.26 nm/sec Measured defocus = 0.17 microns changed by -1.57 to target Tilt-induced shift is -28.02, 66.13 unbinned pixels, unfiltered CCC is 0.0030 The average drift between shots was -6.0, 2.1 unbinned pixels, 0.17 nm/sec Measured defocus = -1.30 microns changed by -0.10 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1232_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1233_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1234_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1235_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1236_X+0Y+1-0.tif 200.375 Running script 3 at item 122-245 (-479.21, 529.59) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 226.371304 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -14.81, 37.48 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -9.2, 2.0 unbinned pixels, 0.25 nm/sec Measured defocus = -0.73 microns changed by -0.87 to target Tilt-induced shift is -33.89, 78.07 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -4.3, 1.1 unbinned pixels, 0.13 nm/sec Measured defocus = -1.55 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1237_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1238_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1239_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1240_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1241_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1242_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1243_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1244_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1245_X+1Y+1-0.tif 300.859 Running script 3 at item 122-253 (-467.49, 530.67) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 497.173227 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -30.57, 69.23 unbinned pixels, unfiltered CCC is 0.0036 The average drift between shots was -7.9, 2.7 unbinned pixels, 0.23 nm/sec Measured defocus = -1.37 microns changed by -0.43 to target Tilt-induced shift is -38.97, 90.88 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was -4.7, 2.3 unbinned pixels, 0.14 nm/sec Measured defocus = -1.80 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1246_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1247_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1248_X+0Y+1-0.tif 132.765 Running script 3 at item 122-255 (-472.41, 532.60) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 215.903248 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -26.22, 61.87 unbinned pixels, unfiltered CCC is 0.0040 The average drift between shots was -11.9, 4.6 unbinned pixels, 0.35 nm/sec Measured defocus = -1.22 microns changed by -0.78 to target Tilt-induced shift is -42.83, 97.81 unbinned pixels, unfiltered CCC is 0.0037 The average drift between shots was -7.1, 4.4 unbinned pixels, 0.23 nm/sec Measured defocus = -1.94 microns changed by -0.06 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1249_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1250_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1251_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1252_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1253_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1254_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1255_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1256_X+1Y+1-0.tif 278.297 Running script 3 at item 123-1 (-584.88, 539.54) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 166.352844 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 30.73, -69.94 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -3.1, 3.3 unbinned pixels, 0.13 nm/sec Measured defocus = 1.39 microns changed by -3.59 to target Tilt-induced shift is -43.73, 100.93 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -1.1, 6.2 unbinned pixels, 0.17 nm/sec Measured defocus = -2.00 microns changed by -0.20 to target Tilt-induced shift is -47.64, 110.54 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -0.7, 5.4 unbinned pixels, 0.15 nm/sec Measured defocus = -2.19 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1257_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1258_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1259_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1260_X-1Y+0-0.tif Beam was shifted 0.015 microns 213.547 Running script 3 at item 123-3 (-578.30, 542.52) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 227.478979 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -0.09, -0.15 unbinned pixels, unfiltered CCC is 0.0162 The average drift between shots was -8.5, 0.5 unbinned pixels, 0.23 nm/sec Measured defocus = 0.00 microns changed by -1.00 to target Tilt-induced shift is -21.81, 48.54 unbinned pixels, unfiltered CCC is 0.0128 The average drift between shots was -5.4, 6.4 unbinned pixels, 0.22 nm/sec Measured defocus = -0.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1261_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1262_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1263_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1264_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1265_X+0Y+1-0.tif 196.922 Running script 3 at item 123-7 (-591.88, 541.31) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 273.11204 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 84.54, -196.91 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -13.1, 5.9 unbinned pixels, 0.39 nm/sec Measured defocus = 3.87 microns changed by -5.07 to target Tilt-induced shift is -20.48, 46.59 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -6.9, 5.7 unbinned pixels, 0.24 nm/sec Measured defocus = -0.92 microns changed by -0.28 to target Tilt-induced shift is -26.07, 58.99 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -4.3, 4.5 unbinned pixels, 0.18 nm/sec Measured defocus = -1.17 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1266_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1267_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1268_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1269_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1270_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1271_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1272_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1273_X+1Y+1-0.tif 03:44:11 Refine ZLP: additional offset = -1.0 eV; total offset = 4.7 eV 304.422 Running script 3 at item 123-12 (-585.31, 544.24) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 233.403928 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 31.00, -69.29 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -9.6, 3.9 unbinned pixels, 0.29 nm/sec Measured defocus = 1.38 microns changed by -2.78 to target Tilt-induced shift is -26.69, 61.19 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -5.1, 4.4 unbinned pixels, 0.19 nm/sec Measured defocus = -1.21 microns changed by -0.19 to target Tilt-induced shift is -30.52, 68.73 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -2.9, 3.6 unbinned pixels, 0.13 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1274_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1275_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1276_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1277_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1278_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1279_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1280_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1281_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1282_X+1Y+1-0.tif 318.594 Running script 3 at item 123-18 (-571.82, 545.45) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 247.044826 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -46.18, 107.57 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -10.4, 3.2 unbinned pixels, 0.29 nm/sec Measured defocus = -2.13 microns changed by 0.53 to target Tilt-induced shift is -37.79, 84.95 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -5.3, 3.7 unbinned pixels, 0.17 nm/sec Measured defocus = -1.69 microns changed by 0.09 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1283_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1284_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1285_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1286_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1287_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1288_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1289_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1290_X+1Y+1-0.tif 287.735 Running script 3 at item 123-20 (-565.23, 548.40) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 194.927355 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -84.02, 201.93 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -14.0, 1.3 unbinned pixels, 0.37 nm/sec Measured defocus = -3.97 microns changed by 2.17 to target Tilt-induced shift is -45.48, 102.93 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.1, 1.1 unbinned pixels, 0.09 nm/sec Measured defocus = -2.04 microns changed by 0.24 to target Tilt-induced shift is -41.42, 92.20 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -2.9, 1.1 unbinned pixels, 0.09 nm/sec Measured defocus = -1.84 microns changed by 0.04 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1291_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1292_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1293_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1294_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1295_X+1Y+1-0.tif 207.437 Running script 3 at item 123-24 (-578.84, 547.18) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 423.723613 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -1.85, 4.15 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -5.8, -5.8 unbinned pixels, 0.22 nm/sec Measured defocus = -0.08 microns changed by -1.92 to target Tilt-induced shift is -42.09, 94.82 unbinned pixels, unfiltered CCC is -0.0008 The average drift between shots was -7.8, 5.0 unbinned pixels, 0.26 nm/sec Measured defocus = -1.88 microns changed by -0.12 to target Tilt-induced shift is -43.88, 100.94 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -5.4, 3.7 unbinned pixels, 0.18 nm/sec Measured defocus = -2.00 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1296_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1297_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1298_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1299_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1300_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1301_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1302_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1303_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1304_X+1Y+1-0.tif 316.688 Running script 3 at item 123-33 (-596.95, 545.48) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 496.655407 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 113.74, -263.29 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -11.8, 5.3 unbinned pixels, 0.35 nm/sec Measured defocus = 5.18 microns changed by -7.38 to target Tilt-induced shift is -39.49, 91.83 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.6, 3.7 unbinned pixels, 0.21 nm/sec Measured defocus = -1.82 microns changed by -0.38 to target Tilt-induced shift is -46.98, 110.67 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -4.2, 4.1 unbinned pixels, 0.16 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1305_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1306_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1307_X-1Y+0-0.tif 138.547 Running script 3 at item 123-36 (-592.67, 548.26) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 54.51, -120.52 unbinned pixels, unfiltered CCC is 0.0047 The average drift between shots was -14.7, 4.1 unbinned pixels, 0.43 nm/sec Measured defocus = 2.40 microns changed by -3.40 to target Tilt-induced shift is -16.04, 36.11 unbinned pixels, unfiltered CCC is 0.0066 The average drift between shots was -6.0, 4.8 unbinned pixels, 0.21 nm/sec Measured defocus = -0.72 microns changed by -0.28 to target Tilt-induced shift is -21.34, 50.28 unbinned pixels, unfiltered CCC is 0.0064 The average drift between shots was -4.7, 3.8 unbinned pixels, 0.16 nm/sec Measured defocus = -0.99 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1308_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1309_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1310_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1311_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1312_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1313_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1314_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1315_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1316_X+1Y+1-0.tif 322.093 Running script 3 at item 123-52 (-572.62, 552.44) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 354.088914 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -71.39, 165.57 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.6, -4.6 unbinned pixels, 0.20 nm/sec Measured defocus = -3.27 microns changed by 2.07 to target Tilt-induced shift is -29.59, 67.13 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -5.8, 4.9 unbinned pixels, 0.21 nm/sec Measured defocus = -1.33 microns changed by 0.13 to target Tilt-induced shift is -27.18, 60.20 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -2.8, 3.9 unbinned pixels, 0.14 nm/sec Measured defocus = -1.20 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1317_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1318_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1319_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1320_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1321_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1322_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1323_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1324_X+1Y+1-0.tif 287.157 Running script 3 at item 123-57 (-586.08, 551.23) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 20.87, -47.92 unbinned pixels, unfiltered CCC is 0.0691 The average drift between shots was -9.0, -4.1 unbinned pixels, 0.27 nm/sec Measured defocus = 0.95 microns changed by -2.35 to target Tilt-induced shift is -28.30, 64.54 unbinned pixels, unfiltered CCC is 0.0578 The average drift between shots was -8.2, 2.5 unbinned pixels, 0.23 nm/sec Measured defocus = -1.28 microns changed by -0.12 to target Tilt-induced shift is -29.93, 68.77 unbinned pixels, unfiltered CCC is 0.0546 The average drift between shots was -4.0, 2.0 unbinned pixels, 0.13 nm/sec Measured defocus = -1.36 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1325_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1326_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1327_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1328_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1329_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1330_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1331_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1332_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1333_X+1Y+1-0.tif 320.359 Running script 3 at item 123-63 (-599.68, 549.96) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 179.608692 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 125.85, -291.79 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -11.9, 7.5 unbinned pixels, 0.39 nm/sec Measured defocus = 5.74 microns changed by -7.34 to target Tilt-induced shift is -27.22, 63.05 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -7.9, 3.1 unbinned pixels, 0.22 nm/sec Measured defocus = -1.25 microns changed by -0.35 to target Tilt-induced shift is -33.84, 78.37 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -3.9, 3.8 unbinned pixels, 0.15 nm/sec Measured defocus = -1.55 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1334_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1335_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1336_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1337_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1338_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1339_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1340_X+1Y+1-0.tif 248.937 Running script 3 at item 123-74 (-579.60, 554.17) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 446.567473 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -16.42, 42.87 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -11.5, 4.9 unbinned pixels, 0.35 nm/sec Measured defocus = -0.83 microns changed by -0.97 to target Tilt-induced shift is -39.48, 86.91 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -6.0, 5.0 unbinned pixels, 0.21 nm/sec Measured defocus = -1.73 microns changed by -0.07 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1341_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1342_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1343_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1344_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1345_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1346_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1347_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1348_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1349_X+1Y+1-0.tif 303.312 Running script 3 at item 123-80 (-566.07, 555.32) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 172.728289 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -89.82, 209.96 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -7.7, 1.0 unbinned pixels, 0.21 nm/sec Measured defocus = -4.15 microns changed by 2.15 to target Tilt-induced shift is -48.64, 112.13 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -5.2, 3.6 unbinned pixels, 0.18 nm/sec Measured defocus = -2.22 microns changed by 0.22 to target Tilt-induced shift is -43.74, 103.34 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -2.4, 2.6 unbinned pixels, 0.10 nm/sec Measured defocus = -2.04 microns changed by 0.04 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1350_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1351_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1352_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1353_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1354_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1355_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1356_X+1Y+1-0.tif 265.047 Running script 3 at item 123-83 (-561.35, 553.37) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 244.177787 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -130.59, 309.93 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -9.8, -2.0 unbinned pixels, 0.27 nm/sec Measured defocus = -6.13 microns changed by 3.93 to target Tilt-induced shift is -58.11, 129.95 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -5.3, 1.3 unbinned pixels, 0.14 nm/sec Measured defocus = -2.58 microns changed by 0.38 to target Tilt-induced shift is -49.63, 114.04 unbinned pixels, unfiltered CCC is 0.0059 The average drift between shots was -4.3, 0.1 unbinned pixels, 0.12 nm/sec Measured defocus = -2.26 microns changed by 0.06 to target Drift is low enough Starting multiple Records (1 position in 1 hole) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1357_X+0Y+0-0.tif 86.922 Running script 3 at item 123-95 (-593.34, 555.26) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 273.731169 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 87.83, -203.13 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -9.5, 2.8 unbinned pixels, 0.28 nm/sec Measured defocus = 4.00 microns changed by -5.00 to target Tilt-induced shift is -18.46, 41.85 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -6.0, 2.1 unbinned pixels, 0.17 nm/sec Measured defocus = -0.83 microns changed by -0.17 to target Tilt-induced shift is -21.72, 50.10 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -3.9, 3.8 unbinned pixels, 0.15 nm/sec Measured defocus = -0.99 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1358_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1359_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1360_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1361_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1362_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1363_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1364_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1365_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1366_X+1Y+1-0.tif 318.812 Running script 3 at item 123-100 (-600.48, 556.93) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 477.325965 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 99.23, -227.11 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -11.3, 4.2 unbinned pixels, 0.33 nm/sec Measured defocus = 4.48 microns changed by -5.68 to target Tilt-induced shift is -15.89, 38.10 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -4.3, 4.2 unbinned pixels, 0.16 nm/sec Measured defocus = -0.75 microns changed by -0.45 to target Tilt-induced shift is -26.19, 59.59 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -2.6, 4.1 unbinned pixels, 0.13 nm/sec Measured defocus = -1.18 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1367_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1368_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1369_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1370_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1371_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1372_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1373_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1374_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1375_X+1Y+1-0.tif 04:48:02 Refine ZLP: additional offset = -0.4 eV; total offset = 4.3 eV 335.188 Running script 3 at item 123-106 (-586.90, 558.20) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 469.69434 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 8.08, -12.33 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -13.7, 4.6 unbinned pixels, 0.39 nm/sec Measured defocus = 0.26 microns changed by -1.66 to target Tilt-induced shift is -27.67, 63.78 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -4.3, 5.3 unbinned pixels, 0.19 nm/sec Measured defocus = -1.26 microns changed by -0.14 to target Tilt-induced shift is -30.56, 70.80 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -2.8, 5.9 unbinned pixels, 0.18 nm/sec Measured defocus = -1.40 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1376_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1377_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1378_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1379_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1380_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1381_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1382_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1383_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1384_X+1Y+1-0.tif 320.015 Running script 3 at item 123-112 (-573.28, 559.42) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 432.746784 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -53.63, 133.65 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -11.8, -0.8 unbinned pixels, 0.32 nm/sec Measured defocus = -2.61 microns changed by 1.01 to target Tilt-induced shift is -37.17, 85.30 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -2.4, 1.0 unbinned pixels, 0.07 nm/sec Measured defocus = -1.69 microns changed by 0.09 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1385_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1386_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1387_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1388_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1389_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1390_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1391_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1392_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1393_X+1Y+1-0.tif 296.25 Running script 3 at item 123-117 (-566.91, 562.29) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -118.98, 279.76 unbinned pixels, unfiltered CCC is 0.0035 The average drift between shots was -10.4, -1.1 unbinned pixels, 0.28 nm/sec Measured defocus = -5.53 microns changed by 3.73 to target Tilt-induced shift is -47.57, 108.77 unbinned pixels, unfiltered CCC is 0.0038 The average drift between shots was -6.2, 2.1 unbinned pixels, 0.18 nm/sec Measured defocus = -2.16 microns changed by 0.36 to target Tilt-induced shift is -40.10, 92.34 unbinned pixels, unfiltered CCC is 0.0034 The average drift between shots was -4.1, 2.3 unbinned pixels, 0.13 nm/sec Measured defocus = -1.83 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1394_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1395_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1396_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1397_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1398_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1399_X-1Y+1-0.tif 237.921 Running script 3 at item 123-122 (-580.40, 561.14) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 486.545203 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -21.22, 47.53 unbinned pixels, unfiltered CCC is 0.0640 The average drift between shots was -4.9, -8.4 unbinned pixels, 0.26 nm/sec Measured defocus = -0.95 microns changed by -1.05 to target Tilt-induced shift is -41.68, 94.48 unbinned pixels, unfiltered CCC is 0.0457 The average drift between shots was -6.0, -0.0 unbinned pixels, 0.16 nm/sec Measured defocus = -1.88 microns changed by -0.12 to target Tilt-induced shift is -43.66, 100.44 unbinned pixels, unfiltered CCC is 0.0423 The average drift between shots was -4.2, -0.1 unbinned pixels, 0.12 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1400_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1401_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1402_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1403_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1404_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1405_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1406_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1407_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1408_X+1Y+1-0.tif 304.969 Running script 3 at item 123-137 (-594.20, 562.20) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 387.349066 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 70.94, -161.86 unbinned pixels, unfiltered CCC is 0.0076 The average drift between shots was -5.0, -6.0 unbinned pixels, 0.21 nm/sec Measured defocus = 3.20 microns changed by -5.40 to target Tilt-induced shift is -38.93, 89.40 unbinned pixels, unfiltered CCC is 0.0127 The average drift between shots was -6.7, 5.1 unbinned pixels, 0.22 nm/sec Measured defocus = -1.77 microns changed by -0.43 to target Tilt-induced shift is -48.67, 110.84 unbinned pixels, unfiltered CCC is 0.0093 The average drift between shots was -2.8, 5.6 unbinned pixels, 0.17 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1409_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1410_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1411_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1412_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1413_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1414_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1415_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1416_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1417_X+1Y+1-0.tif 323.469 Running script 3 at item 123-153 (-574.10, 566.39) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 489.809004 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -70.88, 168.17 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -3.4, -6.3 unbinned pixels, 0.19 nm/sec Measured defocus = -3.31 microns changed by 2.31 to target Tilt-induced shift is -24.41, 53.28 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -4.5, 7.9 unbinned pixels, 0.23 nm/sec Measured defocus = -1.06 microns changed by 0.06 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1418_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1419_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1420_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1421_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1422_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1423_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1424_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1425_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1426_X+1Y+1-0.tif 296.36 Running script 3 at item 123-159 (-587.64, 565.18) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 235.365427 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 38.95, -88.58 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -8.3, 1.9 unbinned pixels, 0.24 nm/sec Measured defocus = 1.76 microns changed by -2.96 to target Tilt-induced shift is -24.18, 55.16 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -5.9, 1.4 unbinned pixels, 0.17 nm/sec Measured defocus = -1.09 microns changed by -0.11 to target Tilt-induced shift is -27.24, 59.85 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -3.7, -0.3 unbinned pixels, 0.11 nm/sec Measured defocus = -1.19 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1427_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1428_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1429_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1430_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1431_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1432_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1433_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1434_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1435_X+1Y+1-0.tif 326.281 Running script 3 at item 123-165 (-601.17, 563.88) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 218.94945 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 117.92, -276.18 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -8.1, 5.0 unbinned pixels, 0.26 nm/sec Measured defocus = 5.42 microns changed by -6.82 to target Tilt-induced shift is -22.45, 51.85 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -5.1, 3.6 unbinned pixels, 0.17 nm/sec Measured defocus = -1.03 microns changed by -0.37 to target Tilt-induced shift is -29.58, 68.41 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.7, 3.6 unbinned pixels, 0.14 nm/sec Measured defocus = -1.35 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1436_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1437_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1438_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1439_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1440_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1441_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1442_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1443_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1444_X+1Y+1-0.tif 323.922 Running script 3 at item 123-177 (-581.07, 568.13) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 492.924486 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -38.82, 95.34 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -9.3, 3.0 unbinned pixels, 0.26 nm/sec Measured defocus = -1.87 microns changed by 0.27 to target Tilt-induced shift is -34.95, 82.12 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.5, 5.7 unbinned pixels, 0.18 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1445_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1446_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1447_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1448_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1449_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1450_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1451_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1452_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1453_X+1Y+1-0.tif 302.031 Running script 3 at item 123-183 (-569.89, 569.06) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -113.37, 265.67 unbinned pixels, unfiltered CCC is 0.0088 The average drift between shots was -12.1, 0.8 unbinned pixels, 0.33 nm/sec Measured defocus = -5.25 microns changed by 3.45 to target Tilt-induced shift is -47.63, 110.03 unbinned pixels, unfiltered CCC is 0.0138 The average drift between shots was -5.6, 3.2 unbinned pixels, 0.17 nm/sec Measured defocus = -2.18 microns changed by 0.38 to target Tilt-induced shift is -40.70, 91.69 unbinned pixels, unfiltered CCC is 0.0152 The average drift between shots was -3.7, 1.7 unbinned pixels, 0.11 nm/sec Measured defocus = -1.82 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1454_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1455_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1456_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1457_X-1Y+1-0.tif 178.14 Running script 3 at item 123-195 (-594.92, 569.17) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 459.79068 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 65.35, -150.06 unbinned pixels, unfiltered CCC is 0.0047 The average drift between shots was -9.4, 0.9 unbinned pixels, 0.25 nm/sec Measured defocus = 2.96 microns changed by -4.96 to target Tilt-induced shift is -41.25, 95.44 unbinned pixels, unfiltered CCC is 0.0045 The average drift between shots was -6.1, 2.9 unbinned pixels, 0.18 nm/sec Measured defocus = -1.89 microns changed by -0.11 to target Tilt-induced shift is -43.00, 100.85 unbinned pixels, unfiltered CCC is 0.0047 The average drift between shots was -3.2, 3.0 unbinned pixels, 0.12 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1458_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1459_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1460_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1461_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1462_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1463_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1464_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1465_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1466_X+1Y+1-0.tif 318.156 Running script 3 at item 123-201 (-602.05, 570.81) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 243.811502 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 82.90, -193.70 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -6.5, -1.4 unbinned pixels, 0.19 nm/sec Measured defocus = 3.81 microns changed by -6.01 to target Tilt-induced shift is -36.79, 86.10 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -4.9, 2.2 unbinned pixels, 0.14 nm/sec Measured defocus = -1.70 microns changed by -0.50 to target Tilt-induced shift is -45.92, 107.41 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.6, 2.7 unbinned pixels, 0.13 nm/sec Measured defocus = -2.12 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1467_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1468_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1469_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1470_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1471_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1472_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1473_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1474_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1475_X+1Y+1-0.tif 05:48:31 Refine ZLP: additional offset = -0.5 eV; total offset = 3.8 eV 340.328 Running script 3 at item 123-207 (-588.45, 572.13) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 203.439238 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 2.14, -4.46 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -2.1, -10.6 unbinned pixels, 0.30 nm/sec Measured defocus = 0.09 microns changed by -1.09 to target Tilt-induced shift is -17.35, 38.41 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.4, 6.9 unbinned pixels, 0.25 nm/sec Measured defocus = -0.77 microns changed by -0.23 to target Tilt-induced shift is -21.53, 50.20 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -3.6, 6.7 unbinned pixels, 0.22 nm/sec Measured defocus = -0.99 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1476_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1477_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1478_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1479_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1480_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1481_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1482_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1483_X+1Y+1-0.tif 290.235 Running script 3 at item 123-212 (-574.90, 573.34) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 421.999728 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -90.58, 216.72 unbinned pixels, unfiltered CCC is 0.7190 The average drift between shots was -6.8, -8.5 unbinned pixels, 0.30 nm/sec Measured defocus = -4.26 microns changed by 3.06 to target Tilt-induced shift is -30.49, 68.04 unbinned pixels, unfiltered CCC is 0.8372 The average drift between shots was -7.2, 1.6 unbinned pixels, 0.20 nm/sec Measured defocus = -1.35 microns changed by 0.15 to target Tilt-induced shift is -27.02, 61.94 unbinned pixels, unfiltered CCC is 0.8400 The average drift between shots was -3.5, 1.2 unbinned pixels, 0.11 nm/sec Measured defocus = -1.23 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1484_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1485_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1486_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1487_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1488_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1489_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1490_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1491_X+1Y+1-0.tif 288.281 Running script 3 at item 123-216 (-570.31, 573.72) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 220.751684 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -97.61, 228.91 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -10.8, 1.6 unbinned pixels, 0.29 nm/sec Measured defocus = -4.52 microns changed by 3.12 to target Tilt-induced shift is -38.25, 89.04 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -4.1, 0.9 unbinned pixels, 0.12 nm/sec Measured defocus = -1.76 microns changed by 0.36 to target Tilt-induced shift is -31.38, 73.06 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -2.8, 0.8 unbinned pixels, 0.08 nm/sec Measured defocus = -1.44 microns changed by 0.04 to target Drift is low enough Starting multiple Records (1 position in 1 hole) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1492_X+0Y+0-0.tif 81.266 Running script 3 at item 123-220 (-581.79, 575.11) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 148.636058 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -16.87, 35.63 unbinned pixels, unfiltered CCC is 0.0025 The average drift between shots was -9.0, 1.5 unbinned pixels, 0.25 nm/sec Measured defocus = -0.72 microns changed by -0.88 to target Tilt-induced shift is -34.22, 79.26 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -5.5, 0.8 unbinned pixels, 0.16 nm/sec Measured defocus = -1.57 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1493_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1494_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1495_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1496_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1497_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1498_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1499_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1500_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1501_X+1Y+1-0.tif 318.203 Running script 3 at item 123-235 (-595.44, 573.82) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 416.095864 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 55.93, -127.05 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -6.5, -1.0 unbinned pixels, 0.18 nm/sec Measured defocus = 2.51 microns changed by -4.31 to target Tilt-induced shift is -34.33, 77.93 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -4.9, 3.7 unbinned pixels, 0.16 nm/sec Measured defocus = -1.55 microns changed by -0.25 to target Tilt-induced shift is -38.94, 90.47 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -3.5, 2.0 unbinned pixels, 0.11 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1502_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1503_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1504_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1505_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1506_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1507_X+1Y+1-0.tif 237.234 Running script 3 at item 123-246 (-575.57, 580.33) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 316.332184 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -92.40, 212.63 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -10.4, 3.7 unbinned pixels, 0.29 nm/sec Measured defocus = -4.21 microns changed by 2.21 to target Tilt-induced shift is -47.72, 111.48 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -6.2, 4.9 unbinned pixels, 0.22 nm/sec Measured defocus = -2.20 microns changed by 0.20 to target Tilt-induced shift is -45.13, 102.83 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -3.7, 2.9 unbinned pixels, 0.13 nm/sec Measured defocus = -2.04 microns changed by 0.04 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1508_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1509_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1510_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1511_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1512_X+0Y+1-0.tif 204.766 Running script 3 at item 123-251 (-588.89, 576.78) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 161.123414 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 11.35, -21.08 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -9.1, -4.9 unbinned pixels, 0.27 nm/sec Measured defocus = 0.43 microns changed by -2.63 to target Tilt-induced shift is -44.88, 102.42 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -5.4, 1.9 unbinned pixels, 0.15 nm/sec Measured defocus = -2.03 microns changed by -0.17 to target Tilt-induced shift is -47.84, 109.74 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.4, 1.2 unbinned pixels, 0.10 nm/sec Measured defocus = -2.17 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1513_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1514_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1515_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1516_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1517_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1518_X+1Y+1-0.tif 229.547 Running script 3 at item 123-260 (-582.36, 579.74) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 416.381058 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -36.21, 85.85 unbinned pixels, unfiltered CCC is 0.0773 The average drift between shots was -4.2, -9.6 unbinned pixels, 0.28 nm/sec Measured defocus = -1.69 microns changed by 0.69 to target Tilt-induced shift is -21.01, 48.79 unbinned pixels, unfiltered CCC is 0.0780 The average drift between shots was -5.3, 3.9 unbinned pixels, 0.18 nm/sec Measured defocus = -0.96 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1519_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1520_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1521_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1522_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1523_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1524_X+1Y+1-0.tif 222.219 Running script 3 at item 124-1 (-407.67, 343.23) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 416.823044 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -2.61, 9.67 unbinned pixels, unfiltered CCC is 0.0233 The average drift between shots was -3.0, -0.1 unbinned pixels, 0.08 nm/sec Measured defocus = -0.18 microns changed by -1.02 to target Tilt-induced shift is -22.94, 52.80 unbinned pixels, unfiltered CCC is 0.0178 The average drift between shots was -3.3, 3.7 unbinned pixels, 0.14 nm/sec Measured defocus = -1.05 microns changed by -0.15 to target Tilt-induced shift is -25.82, 59.25 unbinned pixels, unfiltered CCC is 0.0183 The average drift between shots was -2.9, 3.1 unbinned pixels, 0.11 nm/sec Measured defocus = -1.17 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1525_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1526_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1527_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1528_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1529_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1530_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1531_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1532_X+1Y+1-0.tif Beam was shifted 0.002 microns 312.906 Running script 3 at item 124-3 (-400.66, 343.85) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 203.231414 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -8.07, 22.21 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -7.6, -9.4 unbinned pixels, 0.34 nm/sec Measured defocus = -0.43 microns changed by -0.97 to target Tilt-induced shift is -28.07, 66.37 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -4.2, -0.8 unbinned pixels, 0.12 nm/sec Measured defocus = -1.31 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1533_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1534_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1535_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1536_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1537_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1538_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1539_X+1Y+1-0.tif 241 Running script 3 at item 124-4 (-393.88, 346.80) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 431.576444 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -22.89, 55.00 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -8.7, -4.9 unbinned pixels, 0.28 nm/sec Measured defocus = -1.08 microns changed by -0.52 to target Tilt-induced shift is -34.04, 79.73 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -4.8, -0.7 unbinned pixels, 0.14 nm/sec Measured defocus = -1.57 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1540_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1541_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1542_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1543_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1544_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1545_X+1Y+1-0.tif 222.766 Running script 3 at item 124-11 (-414.88, 344.94) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 173.786205 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 13.24, -27.86 unbinned pixels, unfiltered CCC is 0.0036 The average drift between shots was -8.1, -2.4 unbinned pixels, 0.23 nm/sec Measured defocus = 0.56 microns changed by -2.36 to target Tilt-induced shift is -35.10, 82.64 unbinned pixels, unfiltered CCC is 0.0035 The average drift between shots was -5.7, -0.4 unbinned pixels, 0.15 nm/sec Measured defocus = -1.63 microns changed by -0.17 to target Tilt-induced shift is -39.91, 90.06 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -3.6, 0.6 unbinned pixels, 0.10 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1546_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1547_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1548_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1549_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1550_X+0Y+1-0.tif 202.297 Running script 3 at item 124-13 (-421.99, 346.70) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 328.7534 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 36.06, -86.92 unbinned pixels, unfiltered CCC is 0.0032 The average drift between shots was -5.5, -6.5 unbinned pixels, 0.23 nm/sec Measured defocus = 1.71 microns changed by -3.71 to target Tilt-induced shift is -35.75, 83.56 unbinned pixels, unfiltered CCC is 0.0039 The average drift between shots was -7.4, 4.8 unbinned pixels, 0.24 nm/sec Measured defocus = -1.65 microns changed by -0.35 to target Tilt-induced shift is -42.96, 98.19 unbinned pixels, unfiltered CCC is 0.0030 The average drift between shots was -5.0, 2.3 unbinned pixels, 0.15 nm/sec Measured defocus = -1.95 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1551_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1552_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1553_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1554_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1555_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1556_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1557_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1558_X+1Y+1-0.tif 280.187 Running script 3 at item 124-29 (-401.26, 350.91) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 372.599624 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -3.45, 11.08 unbinned pixels, unfiltered CCC is 0.2110 The average drift between shots was -8.3, 3.1 unbinned pixels, 0.24 nm/sec Measured defocus = -0.21 microns changed by -1.99 to target Tilt-induced shift is -44.73, 104.08 unbinned pixels, unfiltered CCC is 0.1773 The average drift between shots was -5.1, 2.4 unbinned pixels, 0.16 nm/sec Measured defocus = -2.06 microns changed by -0.14 to target Tilt-induced shift is -47.58, 110.00 unbinned pixels, unfiltered CCC is 0.1764 The average drift between shots was -3.5, 2.1 unbinned pixels, 0.11 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1559_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1560_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1561_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1562_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1563_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1564_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1565_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1566_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1567_X+1Y+1-0.tif 304.562 Running script 3 at item 124-32 (-408.30, 350.28) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 275.870632 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -15.79, 38.56 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -8.8, 0.6 unbinned pixels, 0.24 nm/sec Measured defocus = -0.76 microns changed by -0.24 to target Tilt-induced shift is -22.29, 49.24 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.0, 1.0 unbinned pixels, 0.14 nm/sec Measured defocus = -0.98 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1568_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1569_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1570_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1571_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1572_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1573_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1574_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1575_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1576_X+1Y+1-0.tif 06:52:32 Refine ZLP: additional offset = 0.8 eV; total offset = 4.7 eV 323.859 Running script 3 at item 124-35 (-415.32, 349.65) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 358.676662 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 5.72, -11.57 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -7.5, -1.3 unbinned pixels, 0.21 nm/sec Measured defocus = 0.23 microns changed by -1.43 to target Tilt-induced shift is -23.27, 53.02 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -4.9, 2.0 unbinned pixels, 0.15 nm/sec Measured defocus = -1.05 microns changed by -0.15 to target Tilt-induced shift is -26.92, 60.37 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -3.9, 1.0 unbinned pixels, 0.11 nm/sec Measured defocus = -1.20 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1577_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1578_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1579_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1580_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1581_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1582_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1583_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1584_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1585_X+1Y+1-0.tif 307.734 Running script 3 at item 124-41 (-429.41, 350.77) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 287.478411 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 60.10, -141.91 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -9.9, 4.2 unbinned pixels, 0.30 nm/sec Measured defocus = 2.79 microns changed by -4.19 to target Tilt-induced shift is -26.59, 61.17 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -5.8, 1.3 unbinned pixels, 0.16 nm/sec Measured defocus = -1.21 microns changed by -0.19 to target Tilt-induced shift is -29.67, 69.67 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -3.7, 1.5 unbinned pixels, 0.11 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1586_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1587_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1588_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1589_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1590_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1591_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1592_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1593_X+1Y+1-0.tif 277.421 Running script 3 at item 124-55 (-394.55, 353.85) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 295.133203 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -43.53, 103.49 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -9.8, 0.5 unbinned pixels, 0.26 nm/sec Measured defocus = -2.04 microns changed by 0.44 to target Tilt-induced shift is -36.13, 83.03 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -6.0, 4.2 unbinned pixels, 0.20 nm/sec Measured defocus = -1.64 microns changed by 0.04 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1594_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1595_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1596_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1597_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1598_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1599_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1600_X-1Y+1-0.tif 252.11 Running script 3 at item 124-69 (-422.70, 353.73) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 307.869532 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 27.39, -66.17 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -8.0, -3.4 unbinned pixels, 0.24 nm/sec Measured defocus = 1.30 microns changed by -3.10 to target Tilt-induced shift is -34.63, 80.75 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -5.1, 1.2 unbinned pixels, 0.14 nm/sec Measured defocus = -1.60 microns changed by -0.20 to target Tilt-induced shift is -38.75, 89.47 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -2.9, 1.2 unbinned pixels, 0.09 nm/sec Measured defocus = -1.77 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1601_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1602_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1603_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1604_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1605_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1606_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1607_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1608_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1609_X+1Y+1-0.tif 309.469 Running script 3 at item 124-75 (-434.56, 354.96) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 58.39, -133.90 unbinned pixels, unfiltered CCC is 0.1613 The average drift between shots was -13.7, 3.6 unbinned pixels, 0.39 nm/sec Measured defocus = 2.64 microns changed by -4.64 to target Tilt-induced shift is -36.80, 86.01 unbinned pixels, unfiltered CCC is 0.1875 The average drift between shots was -6.9, 1.9 unbinned pixels, 0.20 nm/sec Measured defocus = -1.70 microns changed by -0.30 to target Tilt-induced shift is -42.77, 99.36 unbinned pixels, unfiltered CCC is 0.1762 The average drift between shots was -4.8, 3.2 unbinned pixels, 0.17 nm/sec Measured defocus = -1.96 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1610_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1611_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1612_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1613_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1614_X-1Y+0-0.tif 200.938 Running script 3 at item 124-82 (-415.94, 356.71) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 278.21973 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 3.45, -1.98 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -8.2, 3.6 unbinned pixels, 0.25 nm/sec Measured defocus = 0.06 microns changed by -2.26 to target Tilt-induced shift is -44.79, 102.77 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -5.4, 2.3 unbinned pixels, 0.16 nm/sec Measured defocus = -2.04 microns changed by -0.16 to target Tilt-induced shift is -46.93, 109.91 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -3.1, 2.2 unbinned pixels, 0.11 nm/sec Measured defocus = -2.17 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1615_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1616_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1617_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1618_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1619_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1620_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1621_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1622_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1623_X+1Y+1-0.tif 307.203 Running script 3 at item 124-85 (-408.90, 357.35) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 134.884181 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -14.23, 33.67 unbinned pixels, unfiltered CCC is 0.0217 The average drift between shots was -9.0, 2.7 unbinned pixels, 0.26 nm/sec Measured defocus = -0.66 microns changed by -0.34 to target Tilt-induced shift is -22.29, 50.18 unbinned pixels, unfiltered CCC is 0.0193 The average drift between shots was -4.3, 1.4 unbinned pixels, 0.12 nm/sec Measured defocus = -1.00 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1624_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1625_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1626_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1627_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1628_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1629_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1630_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1631_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1632_X+1Y+1-0.tif 303.14 Running script 3 at item 124-88 (-401.90, 357.96) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 199.983766 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 4.35, -7.97 unbinned pixels, unfiltered CCC is 0.0695 The average drift between shots was -8.2, 3.4 unbinned pixels, 0.24 nm/sec Measured defocus = 0.16 microns changed by -1.36 to target Tilt-induced shift is -23.60, 54.75 unbinned pixels, unfiltered CCC is 0.0564 The average drift between shots was -4.1, 0.9 unbinned pixels, 0.12 nm/sec Measured defocus = -1.08 microns changed by -0.12 to target Tilt-induced shift is -26.13, 60.67 unbinned pixels, unfiltered CCC is 0.0564 The average drift between shots was -3.5, 0.8 unbinned pixels, 0.10 nm/sec Measured defocus = -1.20 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1633_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1634_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1635_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1636_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1637_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1638_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1639_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1640_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1641_X+1Y+1-0.tif 317.328 Running script 3 at item 124-93 (-397.51, 360.70) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 249.864002 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -32.88, 80.97 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -10.5, -1.1 unbinned pixels, 0.29 nm/sec Measured defocus = -1.59 microns changed by 0.19 to target Tilt-induced shift is -29.98, 69.37 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -5.9, 1.7 unbinned pixels, 0.17 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1642_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1643_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1644_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1645_X-1Y+1-0.tif 168.937 Running script 3 at item 124-107 (-430.06, 357.80) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 340.610704 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 74.51, -169.78 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -8.1, -0.3 unbinned pixels, 0.23 nm/sec Measured defocus = 3.35 microns changed by -4.95 to target Tilt-induced shift is -31.08, 73.20 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -5.5, 1.5 unbinned pixels, 0.16 nm/sec Measured defocus = -1.44 microns changed by -0.16 to target Tilt-induced shift is -34.87, 80.25 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -3.2, 1.0 unbinned pixels, 0.09 nm/sec Measured defocus = -1.59 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1646_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1647_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1648_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1649_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1650_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1651_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1652_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1653_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1654_X+1Y+1-0.tif 302.875 Running script 3 at item 124-117 (-423.37, 360.77) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 20.58, -48.82 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -6.9, 0.8 unbinned pixels, 0.19 nm/sec Measured defocus = 0.96 microns changed by -2.76 to target Tilt-induced shift is -33.32, 79.16 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.2, 2.7 unbinned pixels, 0.19 nm/sec Measured defocus = -1.56 microns changed by -0.24 to target Tilt-induced shift is -37.91, 89.77 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.7, 2.6 unbinned pixels, 0.13 nm/sec Measured defocus = -1.77 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1655_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1656_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1657_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1658_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1659_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1660_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1661_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1662_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1663_X+1Y+1-0.tif 312.265 Running script 3 at item 124-131 (-402.57, 365.01) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 268.926686 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -7.24, 18.61 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -9.6, 0.4 unbinned pixels, 0.26 nm/sec Measured defocus = -0.36 microns changed by -1.64 to target Tilt-induced shift is -40.71, 92.12 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -5.5, 2.3 unbinned pixels, 0.16 nm/sec Measured defocus = -1.83 microns changed by -0.17 to target Tilt-induced shift is -43.36, 100.78 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -4.2, 2.7 unbinned pixels, 0.14 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1664_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1665_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1666_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1667_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1668_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1669_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1670_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1671_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1672_X+1Y+1-0.tif 309.937 Running script 3 at item 124-134 (-409.51, 364.41) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 222.836188 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -4.12, 8.33 unbinned pixels, unfiltered CCC is 0.0037 The average drift between shots was -12.1, 2.4 unbinned pixels, 0.34 nm/sec Measured defocus = -0.17 microns changed by -2.03 to target Tilt-induced shift is -45.14, 105.10 unbinned pixels, unfiltered CCC is 0.0031 The average drift between shots was -5.3, 0.3 unbinned pixels, 0.15 nm/sec Measured defocus = -2.08 microns changed by -0.12 to target Tilt-induced shift is -47.63, 111.61 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -4.1, -0.5 unbinned pixels, 0.12 nm/sec Measured defocus = -2.20 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1673_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1674_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1675_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1676_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1677_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1678_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1679_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1680_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1681_X+1Y+1-0.tif 07:55:11 Refine ZLP: additional offset = -0.9 eV; total offset = 3.7 eV 321.375 Running script 3 at item 124-137 (-416.56, 363.78) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 297.507603 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 6.81, -13.68 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -10.4, -3.5 unbinned pixels, 0.30 nm/sec Measured defocus = 0.28 microns changed by -1.28 to target Tilt-induced shift is -18.56, 42.83 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -7.1, 2.9 unbinned pixels, 0.22 nm/sec Measured defocus = -0.85 microns changed by -0.15 to target Tilt-induced shift is -22.05, 49.23 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.6, 2.6 unbinned pixels, 0.15 nm/sec Measured defocus = -0.98 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1682_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1683_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1684_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1685_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1686_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1687_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1688_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1689_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1690_X+1Y+1-0.tif 305.938 Running script 3 at item 124-146 (-435.24, 362.01) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 311.416287 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 67.25, -154.82 unbinned pixels, unfiltered CCC is 0.3125 The average drift between shots was -10.5, 5.0 unbinned pixels, 0.32 nm/sec Measured defocus = 3.05 microns changed by -4.25 to target Tilt-induced shift is -21.56, 49.43 unbinned pixels, unfiltered CCC is 0.4048 The average drift between shots was -5.3, 1.8 unbinned pixels, 0.15 nm/sec Measured defocus = -0.98 microns changed by -0.22 to target Tilt-induced shift is -26.94, 60.54 unbinned pixels, unfiltered CCC is 0.3910 The average drift between shots was -3.1, 1.3 unbinned pixels, 0.09 nm/sec Measured defocus = -1.20 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1691_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1692_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1693_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1694_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1695_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1696_X-1Y+0-0.tif 213.5 Running script 3 at item 124-150 (-430.74, 364.83) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 297.736947 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 48.93, -111.80 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -6.5, -1.8 unbinned pixels, 0.18 nm/sec Measured defocus = 2.21 microns changed by -3.61 to target Tilt-induced shift is -25.33, 58.26 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -5.4, -0.2 unbinned pixels, 0.15 nm/sec Measured defocus = -1.15 microns changed by -0.25 to target Tilt-induced shift is -31.24, 68.81 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -2.6, 2.1 unbinned pixels, 0.09 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1697_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1698_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1699_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1700_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1701_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1702_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1703_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1704_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1705_X+1Y+1-0.tif 313.547 Running script 3 at item 124-165 (-397.91, 365.40) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 427.502219 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -38.09, 91.13 unbinned pixels, unfiltered CCC is 0.0676 The average drift between shots was -9.6, 1.6 unbinned pixels, 0.26 nm/sec Measured defocus = -1.79 microns changed by 0.19 to target Tilt-induced shift is -34.72, 81.18 unbinned pixels, unfiltered CCC is 0.0758 The average drift between shots was -5.8, 3.0 unbinned pixels, 0.17 nm/sec Measured defocus = -1.60 microns changed by 0.00 to target Drift is low enough Starting multiple Records (1 position in 1 hole) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1706_X+0Y+0-0.tif 73.781 Running script 3 at item 124-175 (-423.97, 367.81) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 211.87954 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 26.34, -59.99 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -9.0, -0.5 unbinned pixels, 0.24 nm/sec Measured defocus = 1.19 microns changed by -2.99 to target Tilt-induced shift is -35.79, 84.26 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -4.3, 2.2 unbinned pixels, 0.14 nm/sec Measured defocus = -1.66 microns changed by -0.14 to target Tilt-induced shift is -38.45, 89.49 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -3.8, 1.8 unbinned pixels, 0.12 nm/sec Measured defocus = -1.77 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1707_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1708_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1709_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1710_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1711_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1712_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1713_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1714_X+1Y+1-0.tif 287.125 Running script 3 at item 124-183 (-435.67, 366.67) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 209.856801 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 56.75, -134.36 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -5.1, -6.2 unbinned pixels, 0.22 nm/sec Measured defocus = 2.64 microns changed by -4.64 to target Tilt-induced shift is -35.52, 83.00 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -5.1, 1.5 unbinned pixels, 0.14 nm/sec Measured defocus = -1.64 microns changed by -0.36 to target Tilt-induced shift is -42.84, 100.22 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -3.2, 0.8 unbinned pixels, 0.09 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1715_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1716_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1717_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1718_X+0Y+0-0.tif 161.453 Running script 3 at item 124-190 (-417.19, 370.80) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 150.400796 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -6.12, 11.11 unbinned pixels, unfiltered CCC is 0.0052 The average drift between shots was -7.3, 2.7 unbinned pixels, 0.21 nm/sec Measured defocus = -0.23 microns changed by -1.97 to target Tilt-induced shift is -44.43, 104.32 unbinned pixels, unfiltered CCC is 0.0033 The average drift between shots was -5.2, 1.4 unbinned pixels, 0.15 nm/sec Measured defocus = -2.06 microns changed by -0.14 to target Tilt-induced shift is -47.67, 110.17 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -3.8, 1.6 unbinned pixels, 0.11 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1719_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1720_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1721_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1722_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1723_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1724_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1725_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1726_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1727_X+1Y+1-0.tif 307.532 Running script 3 at item 124-193 (-410.14, 371.43) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 325.224763 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -0.88, 2.30 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -4.5, -2.4 unbinned pixels, 0.14 nm/sec Measured defocus = -0.04 microns changed by -0.96 to target Tilt-induced shift is -21.04, 47.93 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.0, 2.2 unbinned pixels, 0.12 nm/sec Measured defocus = -0.95 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1728_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1729_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1730_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1731_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1732_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1733_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1734_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1735_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1736_X+1Y+1-0.tif 296.032 Running script 3 at item 124-196 (-403.21, 372.06) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 293.64938 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -15.52, 44.92 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -9.0, -7.3 unbinned pixels, 0.30 nm/sec Measured defocus = -0.86 microns changed by -0.34 to target Tilt-induced shift is -24.59, 56.14 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -5.0, 2.5 unbinned pixels, 0.15 nm/sec Measured defocus = -1.11 microns changed by -0.09 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1737_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1738_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1739_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1740_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1741_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1742_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1743_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1744_X+0Y+1-0.tif 274.781 Running script 3 at item 124-211 (-431.20, 369.48) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 185.528915 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 42.37, -94.74 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -9.1, -1.5 unbinned pixels, 0.25 nm/sec Measured defocus = 1.88 microns changed by -3.28 to target Tilt-induced shift is -27.05, 62.27 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -5.5, 0.1 unbinned pixels, 0.14 nm/sec Measured defocus = -1.23 microns changed by -0.17 to target Tilt-induced shift is -30.02, 70.13 unbinned pixels, unfiltered CCC is 0.0031 The average drift between shots was -2.6, 2.0 unbinned pixels, 0.09 nm/sec Measured defocus = -1.39 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1745_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1746_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1747_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1748_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1749_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1750_X+1Y+1-0.tif 221.015 Running script 3 at item 124-219 (-424.39, 372.48) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 243.045632 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 26.76, -56.16 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -12.4, 2.6 unbinned pixels, 0.34 nm/sec Measured defocus = 1.13 microns changed by -2.73 to target Tilt-induced shift is -30.27, 70.58 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -5.7, 2.1 unbinned pixels, 0.17 nm/sec Measured defocus = -1.39 microns changed by -0.21 to target Tilt-induced shift is -34.44, 79.75 unbinned pixels, unfiltered CCC is 0.0025 The average drift between shots was -3.7, 2.6 unbinned pixels, 0.13 nm/sec Measured defocus = -1.58 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1751_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1752_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1753_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1754_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1755_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1756_X+1Y+1-0.tif 215.265 Running script 3 at item 124-230 (-406.23, 378.86) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 392.91236 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -12.60, 29.07 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -7.9, 5.3 unbinned pixels, 0.25 nm/sec Measured defocus = -0.58 microns changed by -1.22 to target Tilt-induced shift is -37.08, 87.46 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -3.8, 0.7 unbinned pixels, 0.11 nm/sec Measured defocus = -1.72 microns changed by -0.08 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1757_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1758_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1759_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1760_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1761_X+0Y+1-0.tif 185.906 Running script 3 at item 124-232 (-410.79, 378.45) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 278.042347 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is -4.59, 7.64 unbinned pixels, unfiltered CCC is 0.0058 The average drift between shots was -7.8, 4.1 unbinned pixels, 0.23 nm/sec Measured defocus = -0.16 microns changed by -1.84 to target Tilt-induced shift is -40.48, 95.58 unbinned pixels, unfiltered CCC is 0.0033 The average drift between shots was -5.6, 1.5 unbinned pixels, 0.16 nm/sec Measured defocus = -1.88 microns changed by -0.12 to target Tilt-induced shift is -44.20, 101.78 unbinned pixels, unfiltered CCC is 0.0029 The average drift between shots was -2.8, -0.3 unbinned pixels, 0.08 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1762_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1763_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1764_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1765_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1766_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1767_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1768_X+1Y+1-0.tif 242.469 Running script 3 at item 124-234 (-417.55, 375.41) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 200.996324 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 11.99, -26.34 unbinned pixels, unfiltered CCC is 0.0031 The average drift between shots was -9.7, 2.9 unbinned pixels, 0.27 nm/sec Measured defocus = 0.53 microns changed by -2.73 to target Tilt-induced shift is -43.68, 102.22 unbinned pixels, unfiltered CCC is 0.0029 The average drift between shots was -6.2, 2.0 unbinned pixels, 0.17 nm/sec Measured defocus = -2.02 microns changed by -0.18 to target Tilt-induced shift is -47.33, 111.48 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -4.2, 0.2 unbinned pixels, 0.11 nm/sec Measured defocus = -2.20 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1769_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1770_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1771_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1772_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1773_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1774_X+1Y+1-0.tif 205.719 Running script 3 at item 125-1 (-412.49, 74.28) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 77.01, -181.01 unbinned pixels, unfiltered CCC is 0.0034 The average drift between shots was -3.3, 1.4 unbinned pixels, 0.10 nm/sec Measured defocus = 3.56 microns changed by -4.56 to target Tilt-induced shift is -15.00, 34.44 unbinned pixels, unfiltered CCC is 0.0088 The average drift between shots was -2.8, 2.3 unbinned pixels, 0.10 nm/sec Measured defocus = -0.68 microns changed by -0.32 to target Tilt-induced shift is -20.95, 47.73 unbinned pixels, unfiltered CCC is 0.0082 The average drift between shots was -2.1, 2.7 unbinned pixels, 0.10 nm/sec Measured defocus = -0.95 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1775_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1776_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1777_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1778_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1779_X+0Y+1-0.tif 08:55:41 Refine ZLP: additional offset = -0.5 eV; total offset = 3.2 eV Beam was shifted 0.022 microns 251.172 Running script 3 at item 125-3 (-405.62, 74.87) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 296.837231 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 75.49, -173.02 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -10.6, 4.9 unbinned pixels, 0.31 nm/sec Measured defocus = 3.41 microns changed by -4.61 to target Tilt-induced shift is -18.93, 44.23 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -5.2, 2.1 unbinned pixels, 0.16 nm/sec Measured defocus = -0.87 microns changed by -0.33 to target Tilt-induced shift is -25.55, 59.55 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -4.5, 2.0 unbinned pixels, 0.13 nm/sec Measured defocus = -1.18 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1780_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1781_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1782_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1783_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1784_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1785_X+0Y+1-0.tif 230.234 Running script 3 at item 125-6 (-398.70, 75.44) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 320.878482 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 23.59, -54.19 unbinned pixels, unfiltered CCC is 0.0972 The average drift between shots was -6.5, -2.2 unbinned pixels, 0.18 nm/sec Measured defocus = 1.07 microns changed by -2.47 to target Tilt-induced shift is -25.81, 58.59 unbinned pixels, unfiltered CCC is 0.0901 The average drift between shots was -6.1, 1.7 unbinned pixels, 0.17 nm/sec Measured defocus = -1.16 microns changed by -0.24 to target Tilt-induced shift is -29.27, 69.16 unbinned pixels, unfiltered CCC is 0.0884 The average drift between shots was -4.6, 1.5 unbinned pixels, 0.14 nm/sec Measured defocus = -1.36 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1786_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1787_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1788_X+0Y+1-0.tif 148.422 Running script 3 at item 125-15 (-415.21, 78.75) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 74.22, -171.01 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -10.7, 0.2 unbinned pixels, 0.30 nm/sec Measured defocus = 3.37 microns changed by -4.97 to target Tilt-induced shift is -27.48, 63.62 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -5.6, 1.0 unbinned pixels, 0.15 nm/sec Measured defocus = -1.26 microns changed by -0.34 to target Tilt-induced shift is -34.53, 78.98 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -3.7, 1.5 unbinned pixels, 0.11 nm/sec Measured defocus = -1.57 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1789_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1790_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1791_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1792_X-1Y+0-0.tif 180.641 Running script 3 at item 125-16 (-410.52, 79.16) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 145.892103 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 56.32, -140.04 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -5.9, 3.8 unbinned pixels, 0.19 nm/sec Measured defocus = 2.73 microns changed by -4.53 to target Tilt-induced shift is -31.12, 72.34 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.4, 1.9 unbinned pixels, 0.18 nm/sec Measured defocus = -1.43 microns changed by -0.37 to target Tilt-induced shift is -38.40, 89.64 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -4.0, 1.1 unbinned pixels, 0.11 nm/sec Measured defocus = -1.77 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1793_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1794_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1795_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1796_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1797_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1798_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1799_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1800_X+0Y+1-0.tif 298.109 Running script 3 at item 125-19 (-403.67, 79.75) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 286.286984 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 56.27, -135.24 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.9, 5.1 unbinned pixels, 0.23 nm/sec Measured defocus = 2.65 microns changed by -4.65 to target Tilt-induced shift is -37.20, 85.25 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -5.0, 1.0 unbinned pixels, 0.13 nm/sec Measured defocus = -1.69 microns changed by -0.31 to target Tilt-induced shift is -42.68, 99.86 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -3.4, 1.7 unbinned pixels, 0.11 nm/sec Measured defocus = -1.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1801_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1802_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1803_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1804_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1805_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1806_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1807_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1808_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1809_X+1Y+1-0.tif 321.438 Running script 3 at item 125-22 (-396.80, 80.29) Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars ARE busy filling LN2 is Refilling Started a long running operation: updating hardware dark reference Call for updating hardware dark reference finished successfully Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 318.462857 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -4.28, 8.49 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -11.3, -2.6 unbinned pixels, 0.33 nm/sec Measured defocus = -0.17 microns changed by -2.03 to target Tilt-induced shift is -44.38, 102.94 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -7.1, 0.7 unbinned pixels, 0.20 nm/sec Measured defocus = -2.04 microns changed by -0.16 to target Tilt-induced shift is -46.67, 108.78 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -6.4, 1.9 unbinned pixels, 0.19 nm/sec Measured defocus = -2.15 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1810_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1811_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1812_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1813_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1814_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1815_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1816_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1817_X+1Y+1-0.tif 279.532 Running script 3 at item 125-25 (-389.86, 80.82) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 479.898442 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -34.63, 80.81 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -8.2, -3.7 unbinned pixels, 0.25 nm/sec Measured defocus = -1.60 microns changed by 0.60 to target Tilt-induced shift is -22.52, 51.52 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.8, 2.2 unbinned pixels, 0.20 nm/sec Measured defocus = -1.02 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1818_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1819_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1820_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1821_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1822_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1823_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1824_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1825_X+1Y+1-0.tif 277.36 Running script 3 at item 125-27 (-385.36, 83.55) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 148.716572 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -58.93, 132.51 unbinned pixels, unfiltered CCC is 0.0709 The average drift between shots was -4.9, -1.4 unbinned pixels, 0.14 nm/sec Measured defocus = -2.63 microns changed by 1.43 to target Tilt-induced shift is -28.51, 66.23 unbinned pixels, unfiltered CCC is 0.1031 The average drift between shots was -5.2, 0.5 unbinned pixels, 0.14 nm/sec Measured defocus = -1.31 microns changed by 0.11 to target Tilt-induced shift is -26.84, 61.14 unbinned pixels, unfiltered CCC is 0.1079 The average drift between shots was -3.8, 0.3 unbinned pixels, 0.11 nm/sec Measured defocus = -1.21 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1826_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1827_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1828_X-1Y+1-0.tif 146.469 Running script 3 at item 125-55 (-378.77, 88.83) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 256.640744 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -93.35, 196.99 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was 3.0, 3.1 unbinned pixels, 0.12 nm/sec Measured defocus = -3.96 microns changed by 2.56 to target Tilt-induced shift is -38.07, 84.06 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -8.1, -1.0 unbinned pixels, 0.22 nm/sec Measured defocus = -1.68 microns changed by 0.28 to target Tilt-induced shift is -33.60, 73.56 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -4.1, -1.1 unbinned pixels, 0.12 nm/sec Measured defocus = -1.47 microns changed by 0.07 to target Drift is low enough Starting multiple Records (1 position in 3 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1829_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1830_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1831_X+1Y+0-0.tif 145.281 Running script 3 at item 125-56 (-383.52, 88.36) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 460.099817 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -79.89, 186.44 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -7.9, -2.2 unbinned pixels, 0.23 nm/sec Measured defocus = -3.68 microns changed by 2.08 to target Tilt-induced shift is -40.17, 92.69 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -5.2, 1.5 unbinned pixels, 0.15 nm/sec Measured defocus = -1.83 microns changed by 0.23 to target Tilt-induced shift is -35.90, 81.90 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -2.7, 0.2 unbinned pixels, 0.08 nm/sec Measured defocus = -1.62 microns changed by 0.02 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1832_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1833_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1834_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1835_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1836_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1837_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1838_X+1Y+1-0.tif 255.922 Running script 3 at item 125-57 (-390.47, 87.80) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 182.90687 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is -42.18, 99.81 unbinned pixels, unfiltered CCC is 0.2794 The average drift between shots was -7.9, -2.9 unbinned pixels, 0.24 nm/sec Measured defocus = -1.97 microns changed by 0.17 to target Tilt-induced shift is -38.92, 89.74 unbinned pixels, unfiltered CCC is 0.2708 The average drift between shots was -4.5, 1.5 unbinned pixels, 0.13 nm/sec Measured defocus = -1.78 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1839_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1840_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1841_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1842_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1843_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1844_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1845_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1846_X+0Y+1-0.tif 269.016 Running script 3 at item 125-60 (-397.35, 87.32) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 211.715006 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 6.94, -9.28 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -13.7, -8.4 unbinned pixels, 0.43 nm/sec Measured defocus = 0.20 microns changed by -2.20 to target Tilt-induced shift is -39.28, 91.34 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -7.0, 2.5 unbinned pixels, 0.20 nm/sec Measured defocus = -1.81 microns changed by -0.19 to target Tilt-induced shift is -44.19, 100.41 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was -4.3, 1.3 unbinned pixels, 0.12 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1847_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1848_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1849_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1850_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1851_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1852_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1853_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1854_X+1Y+1-0.tif 284.016 Running script 3 at item 125-63 (-404.09, 86.81) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 199.705751 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is 43.07, -98.90 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -7.7, 3.4 unbinned pixels, 0.23 nm/sec Measured defocus = 1.96 microns changed by -4.16 to target Tilt-induced shift is -41.79, 97.65 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -5.1, 2.5 unbinned pixels, 0.16 nm/sec Measured defocus = -1.93 microns changed by -0.27 to target Tilt-induced shift is -47.41, 110.51 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -3.2, 1.6 unbinned pixels, 0.10 nm/sec Measured defocus = -2.18 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1855_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1856_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1857_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1858_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1859_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1860_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1861_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1862_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1863_X+1Y+1-0.tif 324.157 Running script 3 at item 125-66 (-411.08, 86.19) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 153.162134 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is 47.02, -106.02 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -7.3, -2.3 unbinned pixels, 0.20 nm/sec Measured defocus = 2.10 microns changed by -3.10 to target Tilt-induced shift is -15.95, 36.71 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.1, 0.9 unbinned pixels, 0.14 nm/sec Measured defocus = -0.73 microns changed by -0.27 to target Tilt-induced shift is -19.69, 49.70 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -5.4, 0.2 unbinned pixels, 0.15 nm/sec Measured defocus = -0.97 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1864_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1865_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1866_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1867_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1868_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1869_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1870_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1871_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1872_X+1Y+1-0.tif 10:01:27 Refine ZLP: additional offset = -0.9 eV; total offset = 2.3 eV 346.703 Running script 3 at item 125-69 (-418.08, 85.57) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is 74.99, -172.22 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -12.0, 2.7 unbinned pixels, 0.33 nm/sec Measured defocus = 3.40 microns changed by -4.60 to target Tilt-induced shift is -18.13, 45.09 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -7.5, 2.5 unbinned pixels, 0.22 nm/sec Measured defocus = -0.88 microns changed by -0.32 to target Tilt-induced shift is -25.29, 58.54 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -4.1, 1.9 unbinned pixels, 0.13 nm/sec Measured defocus = -1.16 microns changed by -0.04 to target Drift is low enough Starting multiple Records (1 position in 7 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1873_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1874_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1875_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1876_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1877_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1878_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1879_X+1Y+1-0.tif 265.328 Running script 3 at item 125-106 (-420.92, 92.39) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is 89.91, -199.65 unbinned pixels, unfiltered CCC is 0.1252 The average drift between shots was -11.3, -4.3 unbinned pixels, 0.33 nm/sec Measured defocus = 3.96 microns changed by -5.36 to target Tilt-induced shift is -19.06, 45.76 unbinned pixels, unfiltered CCC is 0.1317 The average drift between shots was -6.6, 4.1 unbinned pixels, 0.21 nm/sec Measured defocus = -0.90 microns changed by -0.50 to target Tilt-induced shift is -30.15, 69.08 unbinned pixels, unfiltered CCC is 0.1271 The average drift between shots was -3.9, 3.2 unbinned pixels, 0.14 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 4 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1880_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1881_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1882_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1883_X-1Y+0-0.tif 175.844 Running script 3 at item 125-107 (-416.25, 92.82) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 376.999816 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is 73.99, -167.51 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -10.9, 3.6 unbinned pixels, 0.32 nm/sec Measured defocus = 3.31 microns changed by -4.91 to target Tilt-induced shift is -26.72, 61.76 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.4, 2.7 unbinned pixels, 0.19 nm/sec Measured defocus = -1.22 microns changed by -0.38 to target Tilt-induced shift is -35.26, 80.59 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -4.2, 2.1 unbinned pixels, 0.12 nm/sec Measured defocus = -1.60 microns changed by -0.00 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1884_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1885_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1886_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1887_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1888_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1889_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1890_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1891_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1892_X+1Y+1-0.tif 320.75 Running script 3 at item 125-110 (-409.31, 93.44) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 356.665734 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 37.99, -82.90 unbinned pixels, unfiltered CCC is 0.3938 The average drift between shots was -8.9, -3.6 unbinned pixels, 0.25 nm/sec Measured defocus = 1.65 microns changed by -3.45 to target Tilt-induced shift is -32.05, 75.21 unbinned pixels, unfiltered CCC is 0.3740 The average drift between shots was -6.1, 1.0 unbinned pixels, 0.17 nm/sec Measured defocus = -1.48 microns changed by -0.32 to target Tilt-induced shift is -39.85, 90.31 unbinned pixels, unfiltered CCC is 0.3560 The average drift between shots was -2.1, 2.1 unbinned pixels, 0.08 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1893_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1894_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1895_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1896_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1897_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1898_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1899_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1900_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1901_X+1Y+1-0.tif 324.703 Running script 3 at item 125-113 (-402.28, 94.04) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 302.010042 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 21.50, -58.36 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -1.7, -2.0 unbinned pixels, 0.07 nm/sec Measured defocus = 1.13 microns changed by -3.13 to target Tilt-induced shift is -37.60, 88.48 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -6.4, 1.6 unbinned pixels, 0.18 nm/sec Measured defocus = -1.75 microns changed by -0.25 to target Tilt-induced shift is -42.20, 98.72 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -5.2, 2.5 unbinned pixels, 0.16 nm/sec Measured defocus = -1.95 microns changed by -0.05 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1902_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1903_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1904_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1905_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1906_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1907_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1908_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1909_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1910_X+1Y+1-0.tif 326.016 Running script 3 at item 125-116 (-395.49, 94.54) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 254.15267 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2.2 um Tilt-induced shift is -11.34, 33.67 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -9.3, -9.1 unbinned pixels, 0.35 nm/sec Measured defocus = -0.64 microns changed by -1.56 to target Tilt-induced shift is -42.86, 102.14 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -5.2, 0.5 unbinned pixels, 0.14 nm/sec Measured defocus = -2.01 microns changed by -0.19 to target Tilt-induced shift is -48.47, 109.26 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -2.9, 1.6 unbinned pixels, 0.09 nm/sec Measured defocus = -2.17 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1911_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1912_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1913_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1914_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1915_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1916_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1917_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1918_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1919_X+1Y+1-0.tif 306.672 Running script 3 at item 125-119 (-388.64, 95.02) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 267.793011 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1 um Tilt-induced shift is -55.38, 138.36 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -9.2, -13.6 unbinned pixels, 0.45 nm/sec Measured defocus = -2.71 microns changed by 1.71 to target Tilt-induced shift is -21.55, 51.57 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -3.3, 2.9 unbinned pixels, 0.12 nm/sec Measured defocus = -1.01 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 5 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1920_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1921_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1922_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1923_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1924_X+1Y+1-0.tif 198.188 Running script 3 at item 125-121 (-384.01, 95.49) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 179.721928 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.2 um Tilt-induced shift is -89.09, 208.31 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -8.5, -2.1 unbinned pixels, 0.24 nm/sec Measured defocus = -4.11 microns changed by 2.91 to target Tilt-induced shift is -31.29, 71.91 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -5.2, 3.8 unbinned pixels, 0.18 nm/sec Measured defocus = -1.42 microns changed by 0.22 to target Tilt-induced shift is -27.23, 61.06 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -3.6, 2.3 unbinned pixels, 0.12 nm/sec Measured defocus = -1.21 microns changed by 0.01 to target Drift is low enough Starting multiple Records (1 position in 6 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1925_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1926_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1927_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1928_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1929_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1930_X+1Y+1-0.tif 225.593 Running script 3 at item 125-157 (-386.87, 102.36) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.4 um Tilt-induced shift is -74.88, 177.55 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -12.6, 1.4 unbinned pixels, 0.34 nm/sec Measured defocus = -3.50 microns changed by 2.10 to target Tilt-induced shift is -35.16, 82.23 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -7.0, 1.1 unbinned pixels, 0.19 nm/sec Measured defocus = -1.62 microns changed by 0.22 to target Tilt-induced shift is -31.42, 72.30 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -3.8, 1.7 unbinned pixels, 0.12 nm/sec Measured defocus = -1.43 microns changed by 0.03 to target Drift is low enough Starting multiple Records (1 position in 8 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1931_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1932_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1933_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1934_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1935_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1936_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1937_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1938_X+1Y+1-0.tif 293.625 Running script 3 at item 125-159 (-393.68, 101.73) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 198.343446 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.6 um Tilt-induced shift is -24.20, 60.58 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -6.5, -10.2 unbinned pixels, 0.32 nm/sec Measured defocus = -1.18 microns changed by -0.42 to target Tilt-induced shift is -32.26, 73.86 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -5.9, 4.9 unbinned pixels, 0.21 nm/sec Measured defocus = -1.46 microns changed by -0.14 to target Tilt-induced shift is -34.42, 79.36 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.2, 4.4 unbinned pixels, 0.17 nm/sec Measured defocus = -1.57 microns changed by -0.03 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1939_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1940_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1941_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1942_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1943_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1944_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1945_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1946_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1947_X+1Y+1-0.tif 315.781 Running script 3 at item 125-162 (-400.50, 101.26) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 288.743364 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -1.8 um Tilt-induced shift is 2.91, -10.46 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -4.1, 0.1 unbinned pixels, 0.11 nm/sec Measured defocus = 0.20 microns changed by -2.00 to target Tilt-induced shift is -35.58, 82.80 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -5.2, 1.7 unbinned pixels, 0.15 nm/sec Measured defocus = -1.64 microns changed by -0.16 to target Tilt-induced shift is -40.55, 89.79 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -4.7, 0.3 unbinned pixels, 0.13 nm/sec Measured defocus = -1.79 microns changed by -0.01 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1948_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1949_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1950_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1951_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1952_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1953_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1954_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1955_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1956_X+1Y+1-0.tif 315.86 Running script 3 at item 125-165 (-407.53, 100.67) Dewars are NOT busy filling Column/gun valve state is 1 0.0 -0.0 unbinned pixels; 0.0 0.0 nm along two shift axes; -0.0 0.0 nm on specimen axes MoveStage -0 -0 ... Centering detail by moving the stage so that maximum required image shift is 500 nm Detail centered (image shift 212.443524 nm) ===> Running CycleTargetDefocus ... >>>> defined Range and Step (um) => [ -1, -2 ], [ 0.2 ]. TargetDefocus = -2 um Tilt-induced shift is 24.63, -54.49 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -9.0, -2.8 unbinned pixels, 0.25 nm/sec Measured defocus = 1.09 microns changed by -3.09 to target Tilt-induced shift is -37.83, 87.68 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.9, 2.2 unbinned pixels, 0.15 nm/sec Measured defocus = -1.73 microns changed by -0.27 to target Tilt-induced shift is -42.57, 100.19 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -3.2, 1.3 unbinned pixels, 0.10 nm/sec Measured defocus = -1.98 microns changed by -0.02 to target Drift is low enough Starting multiple Records (1 position in 9 holes) WITH beam tilt/astigmatism compensation 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1957_X-1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1958_X+0Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1959_X+1Y-1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1960_X+1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1961_X+0Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1962_X-1Y+0-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1963_X-1Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1964_X+0Y+1-0.tif 100 frames were saved to Z:\users\am2837\20210514\cgAM262_20210514\movies\cgAM262_1965_X+1Y+1-0.tif