Dataset acquisition settings

Calibrated pixel size: 0.729 A
Magnification: 165 000x
Exposure per tilt: 3.5 e/A2
Exposure time: 0.5 s
Camera frames per tilt: 153
Total exposure dose: 115.5 e/A2
Tilt range: +/- 48 deg with 3 deg increment
Scheme: Hagen (dose-symmetric)
Defocus range: -1 to -3 micron
Spot size: 3

Folder structure
frames/ 	- raw camera frames in the EER format
mdoc/ 		- acquisition metafiles, can be used to recreate tilt series from individual images

Instructions: 
Use relion_convert_to_tiff for rendering the EER movies to tiff frames. 
For Warp/M processing we suggest: --eer_grouping 30 --eer_upsampling 2, resulting in 8K frames. 

Gain reference can be converted via selecting the --gain option while executing the relion_convert_to_tiff command. 

Mrc files for the full tilt series can be e.g. exported using Warp, or summed from aligned frames using alignframes function of IMOD, or other implementation of motion correction. 


Relevant information in detail:
https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html
http://www.warpem.com/warp/?page_id=378
https://bio3d.colorado.edu/imod/doc/man/alignframes.html