Read settings from: C:\Program Files\SerialEM\SerialEMsettings.txt Read properties/calibrations from: C:\ProgramData\SerialEM\ SerialEM Version 3.8.0 beta 64-bit, built Jun 20 2019 12:47:27 Started 8/6/2019 10:38:58 Last known C2 aperture size was 50 microns. 7 intensity calibrations have been set to work with that size. Use Calibration - Set Aperture Size if the size is now different or you change the aperture. Read settings from: C:\Program Files\SerialEM\SerialEMsettings20190731..txt Valves are now OPEN The original displacements in the overlap zones have mean 29.46 maximum 57.52 pixels. After shifting pieces into register, the displacements have mean 7.95 maximum 7.95 pixels. Running script 6 at item 7 (202.85, -540.03) Rough eucentricity: changing Z by -44.16 to 55.84, continuing... Rough eucentricity: changing Z by -1.44 to 54.40, finished. Z = 0: The original displacements in the overlap zones have mean 29.21 maximum 43.67 pixels. After shifting pieces into register, the displacements have mean 0.06 maximum 0.06 pixels. Full View shift offset is set to 0.106, 0.209 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to 0.089, -0.405 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to 0.089, -0.022 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to 0.082, -0.218 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Full View shift offset is set to 0.086, -0.161 image shift units Taking a new View image - if feature is not centered, shift to center it and press "Set" again Running script 1 at item 9 (200.58, -539.11) Remaining time to fill dewars is 21120 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Disparity in stage position after aligning to center of map frame: 0.09 0.28 Disparity in stage position after aligning to target position: 0.18 0.50 (2nd move error 0.08 0.20) Finished aligning to item in 2 rounds 4.4 -2.1 unbinned pixels; 13.6 12.7 nm along two shift axes; 7.3 17.6 nm on specimen axes Total_Shift = 9.810676 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 100.67, -65.69 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -4.0, 10.5 unbinned pixels, 0.20 nm/sec Measured defocus = -3.72 microns changed by 0.82 to target 32 frames are being saved to X:\DoseFractions\20190806\slot9_0001.tif Item 9: Stage: 200.58 -539.11 54 Label: 9 9 Tick time from program start = 5229.809 Running script 1 at item 10 (214.57, -547.03) Remaining time to fill dewars is 21000 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: -0.03 0.06 Trimming A by 20 20 45 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.06 0.42 (2nd move error 0.09 0.35) Finished aligning to item in 2 rounds Trimming A by 22 25 40 20, reference by 20 20 45 20 3.8 -3.2 unbinned pixels; 16.8 8.9 nm along two shift axes; 11.7 15.5 nm on specimen axes Total_Shift = 9.976927 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 66.37, -41.17 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -6.4, 13.1 unbinned pixels, 0.26 nm/sec Measured defocus = -2.41 microns changed by -0.59 to target 32 frames are being saved to X:\DoseFractions\20190806\slot9_0002.tif Item 10: Stage: 214.57 -547.03 54 Label: 10 10 Tick time from program start = 5301.912 Valves are now OPEN The original displacements in the overlap zones have mean 17.49 maximum 22.46 pixels. After shifting pieces into register, the displacements have mean 2.26 maximum 2.26 pixels. Running script 6 at item 6 (-351.70, -716.95) Rough eucentricity: changing Z by -95.53 to 4.47, continuing... Rough eucentricity: changing Z by -16.59 to -12.12, finished. Z = 0: The original displacements in the overlap zones have mean 32.44 maximum 47.05 pixels. After shifting pieces into register, the displacements have mean 0.64 maximum 0.64 pixels. Running script 1 at item 8 (-344.21, -711.83) Remaining time to fill dewars is 19560 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.06 -0.11 Disparity in stage position after aligning to target position: 0.07 0.08 (2nd move error -0.01 0.18) Finished aligning to item in 2 rounds 7.8 -2.3 unbinned pixels; 18.5 24.6 nm along two shift axes; 7.1 30.8 nm on specimen axes Total_Shift = 16.207331 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 109.50, -69.92 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -5.8, 6.7 unbinned pixels, 0.16 nm/sec Measured defocus = -4.02 microns changed by 1.22 to target 32 frames are being saved to X:\DoseFractions\20190806\slot10_0001.tif Item 8: Stage: -344.21 -711.83 -12 Label: 8 8 Tick time from program start = 6475.149 Running script 1 at item 9 (-348.59, -721.18) Remaining time to fill dewars is 19500 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.10 -0.08 Disparity in stage position after aligning to target position: -0.07 -0.31 (2nd move error -0.18 -0.25) Finished aligning to item in 2 rounds -21.8 35.1 unbinned pixels; 157.5 25.7 nm along two shift axes; -131.9 -92.3 nm on specimen axes Total_Shift = 82.64694 -1.3 22.1 unbinned pixels; 82.8 28.4 nm along two shift axes; -85.5 -9.8 nm on specimen axes Total_Shift = 44.190047 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 73.71, -48.20 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was 2.3, 4.6 unbinned pixels, 0.09 nm/sec Measured defocus = -2.72 microns changed by -0.18 to target 32 frames are being saved to X:\DoseFractions\20190806\slot10_0002.tif Item 9: Stage: -348.59 -721.18 -12 Label: 9 9 Tick time from program start = 6554.928 Running script 1 at item 10 (-345.91, -713.79) Remaining time to fill dewars is 19440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.14 -0.13 (2nd move error 0.06 -0.04) Finished aligning to item in 1 rounds -5.5 -10.2 unbinned pixels; 30.2 35.2 nm along two shift axes; 40.8 -19.4 nm on specimen axes Total_Shift = 23.175181 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 74.69, -49.27 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was 3.0, 9.4 unbinned pixels, 0.17 nm/sec Measured defocus = -2.76 microns changed by -0.24 to target 32 frames are being saved to X:\DoseFractions\20190806\slot10_0003.tif Item 10: Stage: -345.91 -713.79 -12 Label: 10 10 Tick time from program start = 6619.060 Running script 1 at item 11 (-358.43, -713.57) Remaining time to fill dewars is 19320 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.16 0.07 Aligning to target with image shift equivalent to stage shift: 0.11 -0.09 Finished aligning to item in 1 rounds -9.3 -3.4 unbinned pixels; 0.4 38.7 nm along two shift axes; 15.3 -35.6 nm on specimen axes Total_Shift = 19.870594 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 87.64, -60.58 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was 3.8, 15.2 unbinned pixels, 0.28 nm/sec Measured defocus = -3.29 microns changed by 0.49 to target 32 frames are being saved to X:\DoseFractions\20190806\slot10_0004.tif Item 11: Stage: -358.43 -713.57 -12 Label: 11 11 Tick time from program start = 6681.023 Valves are now OPEN The original displacements in the overlap zones have mean 27.57 maximum 42.17 pixels. After shifting pieces into register, the displacements have mean 2.78 maximum 2.78 pixels. Running script 6 at item 6 (-564.35, 822.39) Rough eucentricity: changing Z by -51.83 to 48.18, continuing... Rough eucentricity: changing Z by -1.49 to 46.68, finished. Z = 0: The original displacements in the overlap zones have mean 13.82 maximum 22.95 pixels. After shifting pieces into register, the displacements have mean 4.95 maximum 4.95 pixels. Running script 1 at item 8 (-569.30, 826.14) Remaining time to fill dewars is 18000 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.20 0.09 Disparity in stage position after aligning to target position: 0.25 0.04 (2nd move error 0.04 -0.07) Finished aligning to item in 2 rounds -7.8 -6.7 unbinned pixels; 14.3 38.3 nm along two shift axes; 27.6 -29.1 nm on specimen axes Total_Shift = 20.587776 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 62.73, -41.51 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was 3.9, 9.9 unbinned pixels, 0.19 nm/sec Measured defocus = -2.32 microns changed by -0.48 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0001.tif Item 8: Stage: -569.30 826.14 47 Label: 8 8 Tick time from program start = 7825.072 Running script 1 at item 9 (-572.76, 821.68) Remaining time to fill dewars is 17820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.34 0.05 (2nd move error 0.13 -0.05) Finished aligning to item in 1 rounds -6.3 -1.6 unbinned pixels; 2.2 25.1 nm along two shift axes; 7.7 -24.1 nm on specimen axes Total_Shift = 12.983199 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 96.69, -65.77 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was 4.8, 14.6 unbinned pixels, 0.28 nm/sec Measured defocus = -3.62 microns changed by 0.72 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0002.tif Item 9: Stage: -572.76 821.68 47 Label: 9 9 Tick time from program start = 7886.568 Running script 1 at item 10 (-572.62, 815.81) Remaining time to fill dewars is 17820 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.22 0.01 Disparity in stage position after aligning to target position: 0.18 -0.01 (2nd move error -0.05 -0.04) Finished aligning to item in 2 rounds -8.9 14.6 unbinned pixels; 65.3 9.9 nm along two shift axes; -55.0 -37.6 nm on specimen axes Total_Shift = 34.189968 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 59.89, -40.21 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -1.7, 6.1 unbinned pixels, 0.11 nm/sec Measured defocus = -2.23 microns changed by -0.77 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0003.tif Item 10: Stage: -572.62 815.81 47 Label: 10 10 Tick time from program start = 7959.748 Running script 1 at item 11 (-567.21, 809.85) Remaining time to fill dewars is 17640 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.35 0.01 (2nd move error 0.12 -0.01) Finished aligning to item in 1 rounds -2.5 -0.2 unbinned pixels; 2.6 9.2 nm along two shift axes; 1.2 -9.7 nm on specimen axes Total_Shift = 5.000852 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 95.68, -63.30 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was 3.1, 10.7 unbinned pixels, 0.20 nm/sec Measured defocus = -3.55 microns changed by 0.75 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0004.tif Item 11: Stage: -567.21 809.85 47 Label: 11 11 Tick time from program start = 8023.974 Running script 6 at item 13 (-457.22, 575.93) Rough eucentricity: changing Z by 7.63 to 54.31, finished. Z = 1: The original displacements in the overlap zones have mean 13.70 maximum 17.68 pixels. After shifting pieces into register, the displacements have mean 0.25 maximum 0.25 pixels. Running script 1 at item 15 (-448.90, 586.12) Remaining time to fill dewars is 17280 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: -0.00 -0.09 Disparity in stage position after aligning to target position: 0.04 -0.02 (2nd move error 0.03 0.05) Finished aligning to item in 2 rounds 0.0 -1.5 unbinned pixels; 5.7 2.3 nm along two shift axes; 6.0 0.4 nm on specimen axes Total_Shift = 3.090241 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 41.11, -26.95 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -3.2, 10.3 unbinned pixels, 0.19 nm/sec Measured defocus = -1.52 microns changed by -1.38 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0005.tif Item 15: Stage: -448.90 586.12 54 Label: 15 15 Tick time from program start = 8341.311 Running script 1 at item 16 (-447.88, 583.75) Remaining time to fill dewars is 17280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.07 -0.01 (2nd move error 0.06 0.06) Finished aligning to item in 1 rounds 0.1 -1.7 unbinned pixels; 6.3 2.3 nm along two shift axes; 6.5 0.6 nm on specimen axes Total_Shift = 3.368135 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 79.90, -51.11 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was 0.0, 13.4 unbinned pixels, 0.24 nm/sec Measured defocus = -2.93 microns changed by -0.07 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0006.tif Item 16: Stage: -447.88 583.75 54 Label: 16 16 Tick time from program start = 8408.579 Running script 1 at item 17 (-454.49, 586.34) Remaining time to fill dewars is 17160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.00 -0.12 (2nd move error -0.00 -0.05) Finished aligning to item in 1 rounds -7.1 2.0 unbinned pixels; 16.6 22.4 nm along two shift axes; -6.4 -27.9 nm on specimen axes Total_Shift = 14.681557 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 35.35, -22.68 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was 1.2, 4.6 unbinned pixels, 0.08 nm/sec Measured defocus = -1.30 microns changed by -1.50 to target Tilt-induced shift is 76.48, -47.90 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was 1.9, 1.4 unbinned pixels, 0.04 nm/sec Measured defocus = -2.79 microns changed by -0.01 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0007.tif Item 17: Stage: -454.49 586.34 54 Label: 17 17 Tick time from program start = 8483.178 Running script 1 at item 18 (-460.03, 586.28) Remaining time to fill dewars is 17100 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.05 0.05 Disparity in stage position after aligning to target position: 0.04 0.13 (2nd move error -0.02 0.07) Finished aligning to item in 2 rounds 2.9 4.0 unbinned pixels; 10.9 16.5 nm along two shift axes; -16.2 10.4 nm on specimen axes Total_Shift = 9.877633 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 66.24, -44.95 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -4.0, 8.8 unbinned pixels, 0.17 nm/sec Measured defocus = -2.47 microns changed by -0.43 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0008.tif Item 18: Stage: -460.03 586.28 54 Label: 18 18 Tick time from program start = 8555.048 The original displacements in the overlap zones have mean 18.89 maximum 24.51 pixels. After shifting pieces into register, the displacements have mean 2.80 maximum 2.80 pixels. Running script 6 at item 8 (-165.68, -237.99) Rough eucentricity: changing Z by -51.62 to 48.38, continuing... Rough eucentricity: changing Z by -2.09 to 46.29, finished. Z = 0: The original displacements in the overlap zones have mean 24.08 maximum 35.11 pixels. After shifting pieces into register, the displacements have mean 0.35 maximum 0.35 pixels. Running script 1 at item 10 (-157.07, -228.96) Remaining time to fill dewars is 15480 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: -0.03 -0.07 Disparity in stage position after aligning to target position: 0.01 0.07 (2nd move error -0.16 0.14) Finished aligning to item in 2 rounds 8.3 31.1 unbinned pixels; 103.5 76.6 nm along two shift axes; -122.7 25.6 nm on specimen axes Total_Shift = 64.360772 0.5 24.6 unbinned pixels; 89.7 38.9 nm along two shift axes; -95.8 -3.1 nm on specimen axes Total_Shift = 49.184926 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 103.99, -68.34 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -2.3, 5.2 unbinned pixels, 0.10 nm/sec Measured defocus = -3.85 microns changed by 0.85 to target 32 frames are being saved to X:\DoseFractions\20190806\slot12_0001.tif Item 10: Stage: -157.07 -228.96 46 Label: 10 10 Tick time from program start = 9957.902 Running script 1 at item 11 (-160.28, -230.47) Remaining time to fill dewars is 15360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.02 -0.07 (2nd move error -0.00 0.01) Finished aligning to item in 1 rounds 2.7 1.4 unbinned pixels; 1.5 11.8 nm along two shift axes; -5.9 10.3 nm on specimen axes Total_Shift = 6.08651 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 61.11, -40.11 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -4.6, 6.2 unbinned pixels, 0.14 nm/sec Measured defocus = -2.26 microns changed by -0.54 to target 32 frames are being saved to X:\DoseFractions\20190806\slot12_0002.tif Item 11: Stage: -160.28 -230.47 46 Label: 11 11 Tick time from program start = 10023.735 Running script 1 at item 12 (-161.06, -232.90) Remaining time to fill dewars is 15300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.01 -0.09 (2nd move error 0.04 -0.03) Finished aligning to item in 1 rounds -5.4 -2.9 unbinned pixels; 3.6 23.9 nm along two shift axes; 12.4 -20.5 nm on specimen axes Total_Shift = 12.323413 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 95.27, -62.55 unbinned pixels, unfiltered CCC is 0.0025 The average drift between shots was 6.0, 11.7 unbinned pixels, 0.24 nm/sec Measured defocus = -3.53 microns changed by 0.63 to target 32 frames are being saved to X:\DoseFractions\20190806\slot12_0003.tif Item 12: Stage: -161.06 -232.90 46 Label: 12 12 Tick time from program start = 10089.521 Running script 1 at item 13 (-174.17, -247.47) Remaining time to fill dewars is 15240 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.10 0.05 Disparity in stage position after aligning to target position: 0.03 -0.08 (2nd move error -0.07 -0.14) Finished aligning to item in 2 rounds -18.6 15.4 unbinned pixels; 80.9 43.8 nm along two shift axes; -56.0 -75.6 nm on specimen axes Total_Shift = 48.305701 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 58.77, -37.82 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -1.6, 5.4 unbinned pixels, 0.10 nm/sec Measured defocus = -2.16 microns changed by -0.84 to target 32 frames are being saved to X:\DoseFractions\20190806\slot12_0004.tif Item 13: Stage: -174.17 -247.47 46 Label: 13 13 Tick time from program start = 10162.841 The original displacements in the overlap zones have mean 24.74 maximum 31.96 pixels. After shifting pieces into register, the displacements have mean 2.09 maximum 2.09 pixels. Running script 6 at item 6 (236.35, 780.43) Trimming A by 25 40, reference by 25 50 Trimming A by 20 25, reference by 25 50 Trimming A by 20 20, reference by 25 50 Trimming A by 20 20, reference by 25 50 Rough eucentricity: changing Z by -6.81 to 93.20, finished. Z = 0: The original displacements in the overlap zones have mean 23.13 maximum 41.23 pixels. After shifting pieces into register, the displacements have mean 6.89 maximum 6.89 pixels. Running script 1 at item 8 (245.61, 785.33) Remaining time to fill dewars is 13860 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 191 21 20 235 Trimming A by 20 20 20 20, reference by 25 20 20 30 Disparity in stage position after aligning to center of map frame: -0.03 -0.05 Disparity in stage position after aligning to target position: 0.13 -0.03 (2nd move error 0.16 0.01) Finished aligning to item in 2 rounds 2.0 -6.9 unbinned pixels; 28.1 3.4 nm along two shift axes; 26.6 9.1 nm on specimen axes Total_Shift = 14.417206 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Rough eucentricity: changing Z by -34.41 to 58.79, continuing... Rough eucentricity: changing Z by -1.95 to 56.84, finished. Changing Z value of 4 items (# 7 - 10) to 56.84 Running script 1 at item 8 (245.61, 785.33) Remaining time to fill dewars is 13680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.06 0.30 (2nd move error 0.08 0.34) Finished aligning to item in 1 rounds 10.5 -6.1 unbinned pixels; 36.0 28.7 nm along two shift axes; 21.3 42.2 nm on specimen axes Total_Shift = 24.269962 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 74.74, -48.38 unbinned pixels, unfiltered CCC is 0.0271 The average drift between shots was -3.9, 11.0 unbinned pixels, 0.21 nm/sec Measured defocus = -2.75 microns changed by -0.05 to target Ending Navigator acquisition after current item 32 frames are being saved to X:\DoseFractions\20190806\slot6_0001.tif Item 8: Stage: 245.61 785.33 57 Label: 8 8 Tick time from program start = 11449.022 Running script 1 at item 9 (244.97, 777.66) Remaining time to fill dewars is 13560 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 191 21 20 235 Trimming A by 20 20 20 20, reference by 25 20 20 30 Disparity in stage position after aligning to center of map frame: 0.03 0.22 Disparity in stage position after aligning to target position: 0.01 0.26 (2nd move error -0.03 0.03) Finished aligning to item in 2 rounds 0.9 6.0 unbinned pixels; 20.8 12.1 nm along two shift axes; -23.4 2.1 nm on specimen axes Total_Shift = 12.083675 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 62.27, -43.01 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -2.2, 6.9 unbinned pixels, 0.13 nm/sec Measured defocus = -2.34 microns changed by -0.56 to target 32 frames are being saved to X:\DoseFractions\20190806\slot6_0002.tif Item 9: Stage: 244.97 777.66 57 Label: 9 9 Tick time from program start = 11637.191 Running script 1 at item 10 (232.75, 781.78) Remaining time to fill dewars is 13440 Dewars are NOT busy filling Trimming A by 200 23 20 230, reference by 191 21 20 235 Trimming A by 200 23 20 230, reference by 20 20 20 20 Trimming A by 200 23 20 230, reference by 25 20 20 30 Trimming A by 200 23 20 230, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: 0.03 0.33 Trimming A by 77 23 20 120, reference by 191 21 20 235 Disparity in stage position after aligning to target position: 0.20 0.32 (2nd move error 0.16 -0.03) Finished aligning to item in 2 rounds Trimming A by 77 22 20 115, reference by 77 23 20 120 -3.2 -3.0 unbinned pixels; 6.9 16.0 nm along two shift axes; 12.4 -11.8 nm on specimen axes Total_Shift = 8.770101 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 92.40, -63.21 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was 2.7, 16.4 unbinned pixels, 0.30 nm/sec Measured defocus = -3.46 microns changed by 0.46 to target 32 frames are being saved to X:\DoseFractions\20190806\slot6_0003.tif Item 10: Stage: 232.75 781.78 57 Label: 10 10 Tick time from program start = 11713.444 Running script 1 at item 11 (231.77, 771.48) Remaining time to fill dewars is 13320 Dewars are NOT busy filling Trimming A by 25 21 20 35, reference by 191 21 20 235 Trimming A by 25 21 20 35, reference by 20 20 20 20 Trimming A by 25 21 20 35, reference by 25 20 20 30 Trimming A by 25 21 20 35, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: 0.11 0.31 Trimming A by 20 20 20 20, reference by 25 20 20 30 Disparity in stage position after aligning to target position: 0.12 0.32 (2nd move error 0.01 -0.00) Finished aligning to item in 2 rounds 0.5 0.5 unbinned pixels; 1.0 2.5 nm along two shift axes; -1.9 1.8 nm on specimen axes Total_Shift = 1.351586 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 48.90, -30.74 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -3.5, 8.7 unbinned pixels, 0.17 nm/sec Measured defocus = -1.79 microns changed by -1.01 to target 32 frames are being saved to X:\DoseFractions\20190806\slot6_0004.tif Item 11: Stage: 231.77 771.48 57 Label: 11 11 Tick time from program start = 11786.219 Valves are now OPEN WARNING: The image shifts for the selected magnification have not been calibrated in the last day. You may want to do this calibration (see Calibration menu) if image shift calibration is not very stable on your scope The original displacements in the overlap zones have mean 14.75 maximum 19.66 pixels. After shifting pieces into register, the displacements have mean 0.88 maximum 0.88 pixels. THIS IS GREAT! The original displacements in the overlap zones have mean 19.45 maximum 21.64 pixels. After shifting pieces into register, the displacements have mean 0.84 maximum 0.84 pixels. Running script 6 at item 7 (-101.97, 377.01) Rough eucentricity: changing Z by -61.12 to 38.88, continuing... Rough eucentricity: changing Z by -1.85 to 37.04, finished. Z = 0: The original displacements in the overlap zones have mean 18.28 maximum 23.16 pixels. After shifting pieces into register, the displacements have mean 0.09 maximum 0.09 pixels. Running script 1 at item 10 (-98.66, 383.97) Remaining time to fill dewars is 11940 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 20 20 20 20, reference by 21 31 65 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.02 Disparity in stage position after aligning to target position: -0.01 -0.10 (2nd move error -0.05 -0.09) Finished aligning to item in 2 rounds -11.3 9.5 unbinned pixels; 49.7 26.6 nm along two shift axes; -34.5 -46.1 nm on specimen axes Total_Shift = 29.562972 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 107.69, -69.71 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was 3.6, 5.8 unbinned pixels, 0.12 nm/sec Measured defocus = -3.97 microns changed by 1.07 to target 32 frames are being saved to X:\DoseFractions\20190806\slot8_0001.tif Item 9: Stage: -98.66 383.97 37 Label: 10 9 Tick time from program start = 13025.413 Running script 1 at item 11 (-99.62, 380.44) Remaining time to fill dewars is 11820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.08 -0.14 (2nd move error 0.04 -0.13) Finished aligning to item in 1 rounds -17.6 -0.6 unbinned pixels; 20.6 64.2 nm along two shift axes; 6.2 -68.2 nm on specimen axes Total_Shift = 35.175819 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 72.45, -45.48 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was 2.5, 9.1 unbinned pixels, 0.17 nm/sec Measured defocus = -2.64 microns changed by -0.36 to target 32 frames are being saved to X:\DoseFractions\20190806\slot8_0002.tif Item 10: Stage: -99.62 380.44 37 Label: 11 10 Tick time from program start = 13091.136 Running script 1 at item 12 (-104.07, 377.60) Remaining time to fill dewars is 11700 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 21 31 65 20 Disparity in stage position after aligning to center of map frame: 0.09 0.07 Disparity in stage position after aligning to target position: 0.34 0.20 (2nd move error 0.24 0.11) Finished aligning to item in 2 rounds 3.4 -1.6 unbinned pixels; 10.3 9.7 nm along two shift axes; 5.6 13.4 nm on specimen axes Total_Shift = 7.446793 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 98.44, -63.53 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.2, 10.7 unbinned pixels, 0.20 nm/sec Measured defocus = -3.63 microns changed by 0.83 to target 32 frames are being saved to X:\DoseFractions\20190806\slot8_0003.tif Item 11: Stage: -104.07 377.60 37 Label: 12 11 Tick time from program start = 13167.405 Running script 1 at item 13 (-106.87, 372.98) Remaining time to fill dewars is 11640 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 21 31 65 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.03 Disparity in stage position after aligning to target position: 0.11 0.01 (2nd move error -0.03 0.02) Finished aligning to item in 2 rounds 1.6 8.0 unbinned pixels; 27.1 17.9 nm along two shift axes; -31.3 4.7 nm on specimen axes Total_Shift = 16.235994 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 60.14, -36.06 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -7.7, 3.9 unbinned pixels, 0.15 nm/sec Measured defocus = -2.17 microns changed by -0.73 to target 32 frames are being saved to X:\DoseFractions\20190806\slot8_0004.tif Item 12: Stage: -106.87 372.98 37 Label: 13 12 Tick time from program start = 13240.366 The original displacements in the overlap zones have mean 18.56 maximum 73.45 pixels. After shifting pieces into register, the displacements have mean 11.44 maximum 50.92 pixels. Running script 6 at item 21 (-788.90, 623.62) Rough eucentricity: changing Z by 21.72 to 71.71, continuing... Rough eucentricity: changing Z by -1.56 to 70.15, finished. Z = 0: The original displacements in the overlap zones have mean 12.60 maximum 19.81 pixels. After shifting pieces into register, the displacements have mean 0.98 maximum 3.59 pixels. Running script 6 at item 28 (-201.42, 500.98) Rough eucentricity: changing Z by 31.59 to 81.61, continuing... Rough eucentricity: changing Z by -1.33 to 80.28, finished. Z = 1: The original displacements in the overlap zones have mean 14.15 maximum 19.15 pixels. After shifting pieces into register, the displacements have mean 0.69 maximum 1.83 pixels. Running script 6 at item 30 (-277.40, 407.68) Rough eucentricity: changing Z by 29.54 to 79.55, continuing... Rough eucentricity: changing Z by -1.70 to 77.85, finished. Z = 2: The original displacements in the overlap zones have mean 15.70 maximum 29.30 pixels. After shifting pieces into register, the displacements have mean 0.71 maximum 2.59 pixels. Running script 6 at item 31 (-268.14, 322.36) Rough eucentricity: changing Z by -12.10 to 37.92, finished. Z = 3: The original displacements in the overlap zones have mean 27.01 maximum 34.82 pixels. After shifting pieces into register, the displacements have mean 0.44 maximum 1.22 pixels. Running script 6 at item 33 (265.57, -233.79) Rough eucentricity: changing Z by 25.00 to 75.00, continuing... Rough eucentricity: changing Z by -0.94 to 74.06, finished. Z = 4: The original displacements in the overlap zones have mean 21.83 maximum 41.14 pixels. After shifting pieces into register, the displacements have mean 0.85 maximum 3.96 pixels. Running script 1 at item 44 (-774.58, 644.64) Remaining time to fill dewars is 7260 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.08 -0.02 Disparity in stage position after aligning to target position: 0.11 -0.09 (2nd move error 0.02 -0.09) Finished aligning to item in 2 rounds -14.1 0.3 unbinned pixels; 19.4 50.3 nm along two shift axes; 1.9 -54.9 nm on specimen axes Total_Shift = 28.18338 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 100.66, -66.96 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was 1.2, 12.9 unbinned pixels, 0.23 nm/sec Measured defocus = -3.74 microns changed by 0.74 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0011.tif Item 41: Stage: -774.58 644.64 70 Label: 44 41 Tick time from program start = 17648.159 Running script 1 at item 45 (-775.88, 641.31) Remaining time to fill dewars is 7260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.11 -0.11 (2nd move error 0.02 -0.11) Finished aligning to item in 1 rounds -13.2 -1.3 unbinned pixels; 12.4 49.5 nm along two shift axes; 7.9 -51.1 nm on specimen axes Total_Shift = 26.540248 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 64.21, -40.36 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was 0.9, 10.8 unbinned pixels, 0.18 nm/sec Measured defocus = -2.34 microns changed by -0.46 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0012.tif Item 42: Stage: -775.88 641.31 70 Label: 45 42 Tick time from program start = 17715.411 Running script 1 at item 46 (-774.71, 638.99) Remaining time to fill dewars is 7140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.26 -0.12 (2nd move error 0.16 -0.12) Finished aligning to item in 1 rounds -12.1 -2.8 unbinned pixels; 5.6 47.9 nm along two shift axes; 13.4 -46.7 nm on specimen axes Total_Shift = 24.916747 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 101.00, -67.76 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was 3.8, 14.0 unbinned pixels, 0.25 nm/sec Measured defocus = -3.76 microns changed by 0.86 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0013.tif Item 43: Stage: -774.71 638.99 70 Label: 46 43 Tick time from program start = 17782.569 Running script 1 at item 47 (-777.21, 643.35) Remaining time to fill dewars is 7020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.20 -0.13 (2nd move error 0.11 -0.13) Finished aligning to item in 1 rounds -14.7 -1.4 unbinned pixels; 14.1 55.0 nm along two shift axes; 8.5 -56.9 nm on specimen axes Total_Shift = 29.532975 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 66.31, -44.84 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was 1.4, 8.6 unbinned pixels, 0.15 nm/sec Measured defocus = -2.47 microns changed by -0.53 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0014.tif Item 44: Stage: -777.21 643.35 70 Label: 47 44 Tick time from program start = 17891.801 Running script 1 at item 48 (-183.27, 518.49) Remaining time to fill dewars is 6780 Dewars are NOT busy filling Trimming A by 115 21 70 20, reference by 80 20 20 45 Trimming A by 115 21 70 20, reference by 105 22 70 20 Trimming A by 115 21 70 20, reference by 20 20 20 20 Trimming A by 115 21 70 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.28 Trimming A by 20 20 20 20, reference by 105 22 70 20 Disparity in stage position after aligning to target position: 0.16 -0.28 (2nd move error 0.10 -0.01) Finished aligning to item in 2 rounds -2.9 -3.2 unbinned pixels; 7.9 15.1 nm along two shift axes; 13.0 -10.4 nm on specimen axes Total_Shift = 8.53989 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 92.39, -64.14 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was 12.0, 10.2 unbinned pixels, 0.27 nm/sec Measured defocus = -3.48 microns changed by 0.68 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0015.tif Item 45: Stage: -183.27 518.49 80 Label: 48 45 Tick time from program start = 18098.393 Running script 1 at item 49 (-188.89, 501.15) Remaining time to fill dewars is 6720 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 75 55 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.17 Disparity in stage position after aligning to target position: 0.10 -0.03 (2nd move error 0.07 0.13) Finished aligning to item in 2 rounds 2.5 1.3 unbinned pixels; 1.4 11.1 nm along two shift axes; -5.5 9.7 nm on specimen axes Total_Shift = 5.70099 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 52.33, -34.07 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was 0.4, 9.0 unbinned pixels, 0.15 nm/sec Measured defocus = -1.93 microns changed by -0.87 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0016.tif Item 46: Stage: -188.89 501.15 80 Label: 49 46 Tick time from program start = 18173.882 Running script 1 at item 50 (-177.83, 506.69) Remaining time to fill dewars is 6600 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 80 20 20 45 Trimming A by 20 20 20 20, reference by 105 22 70 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.23 Trimming A by 20 20 70 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.16 -0.10 (2nd move error 0.10 0.11) Finished aligning to item in 2 rounds Trimming A by 20 20 70 20, reference by 20 20 70 20 1.9 -0.2 unbinned pixels; 3.0 6.6 nm along two shift axes; 0.2 7.4 nm on specimen axes Total_Shift = 3.822689 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 92.54, -61.06 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -1.4, 13.5 unbinned pixels, 0.23 nm/sec Measured defocus = -3.43 microns changed by 0.53 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0017.tif Item 47: Stage: -177.83 506.69 80 Label: 50 47 Tick time from program start = 18251.883 Running script 1 at item 51 (-266.48, 425.90) Remaining time to fill dewars is 6480 Dewars are NOT busy filling Trimming A by 70 20 20 20, reference by 90 20 20 45 Trimming A by 70 20 20 20, reference by 60 20 20 20 Trimming A by 70 20 20 20, reference by 100 22 70 20 Trimming A by 70 20 20 20, reference by 20 20 20 20 Trimming A by 70 20 20 20, reference by 20 20 20 20 Trimming A by 70 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.15 Trimming A by 20 20 20 20, reference by 60 20 20 20 Disparity in stage position after aligning to target position: 0.23 0.00 (2nd move error 0.12 0.14) Finished aligning to item in 2 rounds 1.4 -1.3 unbinned pixels; 6.5 3.0 nm along two shift axes; 4.7 5.6 nm on specimen axes Total_Shift = 3.73847 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 64.99, -44.20 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -6.4, 21.4 unbinned pixels, 0.38 nm/sec Measured defocus = -2.43 microns changed by -0.57 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0018.tif Item 48: Stage: -266.48 425.90 78 Label: 51 48 Tick time from program start = 18388.415 Running script 1 at item 52 (-265.34, 423.75) Remaining time to fill dewars is 6420 Dewars are NOT busy filling Trimming A by 105 22 70 20, reference by 60 20 20 20 Trimming A by 105 22 70 20, reference by 100 22 70 20 Trimming A by 105 22 70 20, reference by 20 20 20 20 Trimming A by 105 22 70 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.21 Trimming A by 20 20 20 20, reference by 100 22 70 20 Disparity in stage position after aligning to target position: 0.12 -0.38 (2nd move error 0.02 -0.19) Finished aligning to item in 2 rounds -26.1 -1.1 unbinned pixels; 30.2 95.6 nm along two shift axes; 9.7 -101.4 nm on specimen axes Total_Shift = 52.292225 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 94.53, -65.50 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was 8.3, 15.9 unbinned pixels, 0.30 nm/sec Measured defocus = -3.55 microns changed by 0.75 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0019.tif Item 49: Stage: -265.34 423.75 78 Label: 52 49 Tick time from program start = 18463.311 Running script 1 at item 53 (-259.72, 340.98) Remaining time to fill dewars is 6300 Dewars are NOT busy filling Trimming A by 40 20 20 20, reference by 60 20 20 20 Trimming A by 40 20 20 20, reference by 30 20 20 20 Trimming A by 40 20 20 20, reference by 112 23 140 20 Trimming A by 40 20 20 20, reference by 20 20 20 20 Trimming A by 40 20 20 20, reference by 20 20 20 20 Trimming A by 40 20 20 20, reference by 20 20 25 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.22 Trimming A by 20 20 20 20, reference by 30 20 20 20 Disparity in stage position after aligning to target position: 0.14 -0.06 (2nd move error 0.08 0.15) Finished aligning to item in 2 rounds 2.2 3.2 unbinned pixels; 9.0 12.9 nm along two shift axes; -13.1 8.0 nm on specimen axes Total_Shift = 7.860846 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Rough eucentricity: changing Z by 41.38 to 79.31, continuing... Rough eucentricity: changing Z by -2.39 to 76.92, finished. Changing Z value of 2 items (# 49 - 50) to 76.92 Running script 1 at item 53 (-259.72, 340.98) Remaining time to fill dewars is 6120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 30 20 20 20 Disparity in stage position after aligning to target position: 0.30 -0.41 (2nd move error 0.25 -0.20) Finished aligning to item in 1 rounds -19.3 4.4 unbinned pixels; 41.4 62.9 nm along two shift axes; -13.1 -76.1 nm on specimen axes Total_Shift = 39.631372 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 77.98, -49.93 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was 6.8, 10.7 unbinned pixels, 0.21 nm/sec Measured defocus = -2.86 microns changed by -0.14 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0020.tif Item 50: Stage: -259.72 340.98 77 Label: 53 50 Tick time from program start = 18749.214 Running script 1 at item 54 (-258.81, 338.57) Remaining time to fill dewars is 6000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 30 20 20 20 Disparity in stage position after aligning to target position: 0.35 -0.39 (2nd move error 0.30 -0.19) Finished aligning to item in 1 rounds -18.0 -3.4 unbinned pixels; 11.1 69.9 nm along two shift axes; 17.0 -69.3 nm on specimen axes Total_Shift = 36.629107 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 71.51, -46.15 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was 8.2, 12.7 unbinned pixels, 0.26 nm/sec Measured defocus = -2.63 microns changed by -0.17 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0021.tif Item 51: Stage: -258.81 338.57 77 Label: 54 51 Tick time from program start = 18816.965 Running script 1 at item 55 (277.80, -217.06) Remaining time to fill dewars is 5940 Dewars are NOT busy filling Trimming A by 219 21 180 20, reference by 140 20 20 40 Trimming A by 219 21 180 20, reference by 220 22 170 20 Trimming A by 219 21 180 20, reference by 20 20 20 20 Trimming A by 219 21 180 20, reference by 20 20 55 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.19 Trimming A by 40 20 25 20, reference by 220 22 170 20 Disparity in stage position after aligning to target position: 0.16 -0.41 (2nd move error 0.04 -0.25) Finished aligning to item in 2 rounds Trimming A by 40 20 20 20, reference by 40 20 25 20 -28.9 -2.0 unbinned pixels; 30.4 107.2 nm along two shift axes; 13.9 -112.2 nm on specimen axes Total_Shift = 58.016963 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 84.13, -54.08 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was 3.6, 15.8 unbinned pixels, 0.28 nm/sec Measured defocus = -3.09 microns changed by 0.19 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0022.tif Item 52: Stage: 277.80 -217.06 74 Label: 55 52 Tick time from program start = 18935.698 Running script 1 at item 56 (279.10, -219.45) Remaining time to fill dewars is 5820 Dewars are NOT busy filling Trimming A by 20 20 55 20, reference by 140 20 20 40 Trimming A by 20 20 55 20, reference by 220 22 170 20 Trimming A by 20 20 55 20, reference by 20 20 20 20 Trimming A by 20 20 55 20, reference by 20 20 55 20 Trimming A by 20 20 55 20, reference by 20 20 20 20 Trimming A by 20 20 55 20, reference by 21 35 75 20 Disparity in stage position after aligning to center of map frame: 0.10 -0.15 Trimming A by 20 20 20 20, reference by 20 20 55 20 Disparity in stage position after aligning to target position: -0.05 -0.38 (2nd move error -0.15 -0.24) Finished aligning to item in 2 rounds -25.9 36.2 unbinned pixels; 166.8 38.8 nm along two shift axes; -135.2 -108.4 nm on specimen axes Total_Shift = 88.95411 -3.3 22.9 unbinned pixels; 88.4 22.6 nm along two shift axes; -88.3 -17.6 nm on specimen axes Total_Shift = 46.204055 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 82.24, -52.35 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -0.5, 5.3 unbinned pixels, 0.09 nm/sec Measured defocus = -3.01 microns changed by 0.01 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0023.tif Item 53: Stage: 279.10 -219.45 74 Label: 56 53 Tick time from program start = 19018.051 Running script 6 at item 26 (-473.69, 821.59) Rough eucentricity: changing Z by 26.80 to 76.82, continuing... Rough eucentricity: changing Z by -1.32 to 75.49, finished. Z = 5: The original displacements in the overlap zones have mean 10.86 maximum 19.04 pixels. After shifting pieces into register, the displacements have mean 0.50 maximum 1.15 pixels. Running script 1 at item 58 (-451.45, 848.15) Remaining time to fill dewars is 5400 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.01 -0.16 Trimming A by 152 22 185 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.03 -0.05 (2nd move error 0.01 0.09) Finished aligning to item in 2 rounds Trimming A by 160 22 180 20, reference by 152 22 185 20 3.3 0.2 unbinned pixels; 3.6 12.0 nm along two shift axes; -1.4 12.7 nm on specimen axes Total_Shift = 6.549537 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 49.37, -33.09 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -2.9, 9.2 unbinned pixels, 0.16 nm/sec Measured defocus = -1.84 microns changed by -0.96 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0024.tif Item 55: Stage: -451.45 848.15 75 Label: 58 55 Tick time from program start = 19415.619 Running script 1 at item 59 (-449.64, 802.50) Remaining time to fill dewars is 5280 Dewars are NOT busy filling Trimming A by 21 35 30 20, reference by 20 20 20 20 Trimming A by 21 35 30 20, reference by 20 20 20 20 Trimming A by 21 35 30 20, reference by 20 20 20 20 Trimming A by 21 35 30 20, reference by 21 35 35 20 Disparity in stage position after aligning to center of map frame: 0.07 -0.14 Trimming A by 24 90 100 20, reference by 21 35 35 20 Disparity in stage position after aligning to target position: 0.13 -0.03 (2nd move error 0.05 0.09) Finished aligning to item in 2 rounds Trimming A by 24 67 120 20, reference by 24 90 100 20 0.2 -0.6 unbinned pixels; 2.4 0.1 nm along two shift axes; 2.2 1.0 nm on specimen axes Total_Shift = 1.244235 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 93.36, -62.90 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -0.6, 15.2 unbinned pixels, 0.26 nm/sec Measured defocus = -3.48 microns changed by 0.58 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0025.tif Item 56: Stage: -449.64 802.50 75 Label: 59 56 Tick time from program start = 19492.824 Running script 6 at item 60 (38.92, 601.18) Rough eucentricity: changing Z by 34.70 to 84.72, continuing... Rough eucentricity: changing Z by -1.80 to 82.92, finished. Z = 0: The original displacements in the overlap zones have mean 12.25 maximum 18.12 pixels. After shifting pieces into register, the displacements have mean 0.55 maximum 1.17 pixels. Running script 6 at item 61 (-289.36, 578.46) Rough eucentricity: changing Z by 29.92 to 79.94, continuing... Rough eucentricity: changing Z by -1.28 to 78.66, finished. Z = 1: The original displacements in the overlap zones have mean 12.93 maximum 19.30 pixels. After shifting pieces into register, the displacements have mean 4.30 maximum 10.84 pixels. Running script 6 at item 62 (-293.61, 663.94) Rough eucentricity: changing Z by 29.66 to 79.67, continuing... Rough eucentricity: changing Z by -1.28 to 78.39, finished. Z = 2: The original displacements in the overlap zones have mean 13.62 maximum 25.27 pixels. After shifting pieces into register, the displacements have mean 0.79 maximum 6.07 pixels. Running script 6 at item 63 (-376.73, 655.40) Rough eucentricity: changing Z by 28.27 to 78.29, continuing... Rough eucentricity: changing Z by -1.29 to 77.00, finished. Z = 3: The original displacements in the overlap zones have mean 13.46 maximum 31.09 pixels. After shifting pieces into register, the displacements have mean 3.56 maximum 23.75 pixels. Running script 6 at item 64 (-211.06, 670.50) Trimming A by 45 50, reference by 20 20 Trimming A by 20 70, reference by 20 20 Rough eucentricity: changing Z by 31.28 to 81.30, continuing... Trimming A by 21 20 20 75, reference by 22 20 60 75 Trimming A by 22 75, reference by 21 80 Rough eucentricity: changing Z by -1.34 to 79.96, finished. Z = 4: The original displacements in the overlap zones have mean 12.11 maximum 19.66 pixels. After shifting pieces into register, the displacements have mean 0.47 maximum 1.41 pixels. Running script 1 at item 438 (47.58, 606.66) Remaining time to fill dewars is 3300 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.04 -0.16 Disparity in stage position after aligning to target position: 0.10 -0.10 (2nd move error 0.13 0.04) Finished aligning to item in 2 rounds -0.3 -3.1 unbinned pixels; 10.8 5.9 nm along two shift axes; 12.0 -0.7 nm on specimen axes Total_Shift = 6.165665 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 67.57, -44.61 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -1.1, 12.4 unbinned pixels, 0.22 nm/sec Measured defocus = -2.50 microns changed by -0.50 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0026.tif Item 440: Stage: 47.58 606.66 83 Label: 438 440 Tick time from program start = 21427.065 Running script 1 at item 439 (48.60, 604.37) Remaining time to fill dewars is 3180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.10 -0.11 (2nd move error 0.14 0.03) Finished aligning to item in 1 rounds -0.4 0.9 unbinned pixels; 4.0 0.2 nm along two shift axes; -3.5 -1.9 nm on specimen axes Total_Shift = 2.063016 Target defocus is: -3.00 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 99.39, -64.95 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -0.9, 11.0 unbinned pixels, 0.19 nm/sec Measured defocus = -3.67 microns changed by 0.87 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0027.tif Item 441: Stage: 48.60 604.37 83 Label: 439 441 Tick time from program start = 21496.875 Running script 1 at item 440 (56.45, 588.09) Remaining time to fill dewars is 3180 Dewars are NOT busy filling Trimming A by 20 220 115 20, reference by 20 20 20 20 Trimming A by 20 220 115 20, reference by 20 20 20 20 Trimming A by 20 220 115 20, reference by 20 158 20 20 Trimming A by 20 220 115 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.01 -0.09 Trimming A by 20 45 40 20, reference by 21 225 115 20 Disparity in stage position after aligning to target position: -0.13 -0.20 (2nd move error -0.12 -0.13) Finished aligning to item in 2 rounds Trimming A by 21 45 40 20, reference by 20 45 40 20 -15.7 22.7 unbinned pixels; 103.9 22.3 nm along two shift axes; -85.0 -65.8 nm on specimen axes Total_Shift = 55.174822 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.80 um Tilt-induced shift is 58.26, -34.98 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -4.0, 5.4 unbinned pixels, 0.12 nm/sec Measured defocus = -2.10 microns changed by -0.70 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0028.tif Item 442: Stage: 56.45 588.09 83 Label: 440 442 Tick time from program start = 21574.049 Running script 1 at item 441 (-273.39, 588.34) Remaining time to fill dewars is 2940 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 20 Trimming A by 20 20 20 20, reference by 70 20 55 20 Disparity in stage position after aligning to center of map frame: 0.01 -0.10 Disparity in stage position after aligning to target position: -0.06 -0.16 (2nd move error -0.07 -0.08) Finished aligning to item in 2 rounds -9.5 13.7 unbinned pixels; 62.8 13.8 nm along two shift axes; -51.2 -40.0 nm on specimen axes Total_Shift = 33.369032 Target defocus is: -2.80 um 0.00 degrees Target defocus is: -2.90 um Tilt-induced shift is 91.13, -59.09 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was 2.2, 7.4 unbinned pixels, 0.13 nm/sec Measured defocus = -3.36 microns changed by 0.46 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0029.tif Item 443: Stage: -273.39 588.34 79 Label: 441 443 Tick time from program start = 21722.874 Running script 1 at item 442 (-295.89, 559.81) Remaining time to fill dewars is 2940 Dewars are NOT busy filling Trimming A by 20 35 20 20, reference by 20 20 20 85 Trimming A by 20 35 20 20, reference by 20 20 20 20 Trimming A by 20 35 20 20, reference by 20 20 20 20 Trimming A by 20 35 20 20, reference by 22 115 20 160 Trimming A by 20 35 20 20, reference by 20 35 20 20 Trimming A by 20 35 20 20, reference by 20 55 20 40 Disparity in stage position after aligning to center of map frame: -0.05 -0.09 Trimming A by 20 20 20 20, reference by 20 35 20 20 Disparity in stage position after aligning to target position: -0.08 -0.15 (2nd move error -0.06 -0.06) Finished aligning to item in 2 rounds -7.2 10.5 unbinned pixels; 48.2 10.1 nm along two shift axes; -39.5 -30.3 nm on specimen axes Total_Shift = 25.536433 Target defocus is: -2.90 um 0.00 degrees Target defocus is: -3.00 um Tilt-induced shift is 41.85, -26.24 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -2.1, 1.9 unbinned pixels, 0.05 nm/sec Measured defocus = -1.53 microns changed by -1.47 to target 32 frames are being saved to X:\DoseFractions\20190806\slot11_0030.tif Item 444: Stage: -295.89 559.81 79 Label: 442 444 Tick time from program start = 21800.578 32 frames were saved to X:\DoseFractions\20190806\slot11_0031.tif 32 frames were saved to X:\DoseFractions\20190806\slot11_0032.tif 40 frames were saved to X:\DoseFractions\20190806\slot11_0033.tif Rough eucentricity: changing Z by -9.04 to 69.64, finished. Tilt-induced shift is 5.31, -2.51 unbinned pixels, unfiltered CCC is 0.0195 The average drift between shots was -1.1, 1.2 unbinned pixels, 0.02 nm/sec Measured defocus = -0.18 microns Changing defocus to -0.90 to get an acceptable number of CTF rings Ctffind: defocus: -0.897, astig: 0.059 um, angle: -7.0, score 0.8008, fit to 3.2 A Objective stigmator was adjusted by 0.0012 -0.0009 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.434, astig: 0.011 um, angle: -34.1, score 0.7872, fit to 3.1 A Ctffind: defocus: -1.100, astig: 0.343 um, angle: 57.8, score 0.7917, fit to 3.2 A Ctffind: defocus: -1.115, astig: 0.336 um, angle: -75.5, score 0.7938, fit to 3.4 A Ctffind: defocus: -1.110, astig: 0.355 um, angle: -29.1, score 0.7858, fit to 3.2 A Ctffind: defocus: -1.092, astig: 0.366 um, angle: 15.2, score 0.7947, fit to 3.2 A Ctffind: defocus: -1.082, astig: 0.369 um, angle: 59.8, score 0.7981, fit to 3.2 A Ctffind: defocus: -1.085, astig: 0.378 um, angle: -75.8, score 0.7989, fit to 3.1 A Ctffind: defocus: -1.092, astig: 0.376 um, angle: -32.2, score 0.7972, fit to 3.2 A Ctffind: defocus: -1.101, astig: 0.361 um, angle: 12.4, score 0.7912, fit to 3.2 A Ctffind: defocus: -1.464, astig: 0.012 um, angle: -35.3, score 0.7908, fit to 3.2 A Beam tilt was adjusted by -0.12 -0.09 milliradians Tilt-induced shift is 17.90, -10.58 unbinned pixels, unfiltered CCC is 0.0110 The average drift between shots was -1.7, -3.3 unbinned pixels, 0.04 nm/sec Measured defocus = -0.64 microns Changing defocus to -0.90 to get an acceptable number of CTF rings Ctffind: defocus: -0.988, astig: 0.012 um, angle: 0.6, score 0.8044, fit to 3.2 A Objective stigmator was adjusted by 0.0002 -0.0003 Measuring at image shift -0.4 0.0 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.477, astig: 0.022 um, angle: -65.9, score 0.7807, fit to 3.4 A Ctffind: defocus: -1.123, astig: 0.370 um, angle: 64.3, score 0.7873, fit to 3.2 A Ctffind: defocus: -1.118, astig: 0.361 um, angle: 56.1, score 0.7926, fit to 3.2 A Ctffind: defocus: -1.082, astig: 0.402 um, angle: -31.3, score 0.7889, fit to 3.2 A Ctffind: defocus: -1.172, astig: 0.318 um, angle: -33.2, score 0.7917, fit to 3.3 A Beam tilt needs to be adjusted by -0.04 0.52 milliradians Measuring at image shift 0.4 0.0 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.528, astig: 0.019 um, angle: -1.3, score 0.7802, fit to 3.2 A Ctffind: defocus: -1.184, astig: 0.349 um, angle: 60.8, score 0.7846, fit to 3.1 A Ctffind: defocus: -1.153, astig: 0.376 um, angle: 54.4, score 0.7941, fit to 3.3 A Ctffind: defocus: -1.141, astig: 0.393 um, angle: -27.9, score 0.7935, fit to 3.1 A Ctffind: defocus: -1.209, astig: 0.329 um, angle: -31.1, score 0.7924, fit to 3.2 A Beam tilt needs to be adjusted by -0.18 0.39 milliradians Measuring at image shift 0.0 -0.4 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.511, astig: 0.005 um, angle: -6.6, score 0.7828, fit to 3.5 A Ctffind: defocus: -1.152, astig: 0.367 um, angle: 61.6, score 0.7915, fit to 3.2 A Ctffind: defocus: -1.146, astig: 0.364 um, angle: 55.5, score 0.7937, fit to 3.2 A Ctffind: defocus: -1.120, astig: 0.388 um, angle: -29.8, score 0.7931, fit to 3.2 A Ctffind: defocus: -1.186, astig: 0.327 um, angle: -31.1, score 0.7933, fit to 3.1 A Beam tilt needs to be adjusted by -0.04 0.37 milliradians Measuring at image shift 0.0 0.4 Changing defocus to -1.40 to get an acceptable number of CTF rings Ctffind: defocus: -1.514, astig: 0.012 um, angle: -48.1, score 0.7812, fit to 3.3 A Ctffind: defocus: -1.169, astig: 0.350 um, angle: 63.6, score 0.7978, fit to 3.3 A Ctffind: defocus: -1.135, astig: 0.370 um, angle: 55.4, score 0.7909, fit to 3.2 A Ctffind: defocus: -1.111, astig: 0.405 um, angle: -29.4, score 0.7900, fit to 3.2 A Ctffind: defocus: -1.202, astig: 0.324 um, angle: -33.1, score 0.7936, fit to 3.2 A Beam tilt needs to be adjusted by -0.20 0.51 milliradians IS to beam tilt matrix: -0.176619 -0.197919 -0.168793 0.179054 Running script 17 at item 613 (-308.62, 656.72) Remaining time to fill dewars is 31140 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 21 55 20 60 Disparity in stage position after aligning to center of map frame: -0.17 -0.20 Disparity in stage position after aligning to target position: -0.09 -0.11 (2nd move error 0.07 0.07) Finished aligning to item in 2 rounds -1.3 -0.7 unbinned pixels; 1.1 5.8 nm along two shift axes; 3.2 -4.9 nm on specimen axes Total_Shift = 2.981269 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 41.11, -27.07 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -1.8, 8.4 unbinned pixels, 0.15 nm/sec Measured defocus = -1.52 microns changed by 0.22 to target 32 frames were saved to X:\DoseFractions\20190806\slot11_0034.tif 32 frames were saved to X:\DoseFractions\20190806\slot11_0035.tif 32 frames were saved to X:\DoseFractions\20190806\slot11_0036.tif Item 615: Stage: -308.62 656.72 78 Label: 613 615 Tick time from program start = 25504.650 Running script 17 at item 65-1 (-767.92, 636.47) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.09 -0.22 Disparity in stage position after aligning to target position: 0.02 -0.17 (2nd move error -0.07 0.02) Finished aligning to item in 2 rounds -0.5 14.2 unbinned pixels; 52.9 19.6 nm along two shift axes; -55.2 -5.0 nm on specimen axes Total_Shift = 28.452314 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 18.17, -8.28 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -16.5, 11.3 unbinned pixels, 0.35 nm/sec Measured defocus = -0.61 microns changed by -0.79 to target 34 frames were saved to U:\krios\20190806\slot11_0011.tif 34 frames were saved to U:\krios\20190806\slot11_0012.tif 34 frames were saved to U:\krios\20190806\slot11_0013.tif Item 67: Stage: -767.92 636.47 70 Label: 65-1 67 Tick time from program start = 25792.066 Running script 17 at item 66-1 (-766.85, 634.12) Remaining time to fill dewars is 30840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 35 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.21 -0.19 (2nd move error 0.11 0.01) Finished aligning to item in 1 rounds Trimming A by 20 20 40 20, reference by 20 20 35 20 1.7 -0.6 unbinned pixels; 4.4 5.4 nm along two shift axes; 1.9 6.9 nm on specimen axes Total_Shift = 3.670989 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 19.96, -10.72 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -9.0, 8.8 unbinned pixels, 0.22 nm/sec Measured defocus = -0.70 microns changed by -0.80 to target 34 frames were saved to U:\krios\20190806\slot11_0014.tif 34 frames were saved to U:\krios\20190806\slot11_0015.tif Ending Navigator acquisition after current item 34 frames were saved to U:\krios\20190806\slot11_0016.tif Item 68: Stage: -766.85 634.12 70 Label: 66-1 68 Tick time from program start = 25910.643 Running script 17 at item 67-1 (-765.78, 631.79) Remaining time to fill dewars is 30660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 65 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.23 -0.16 (2nd move error 0.14 0.03) Finished aligning to item in 1 rounds Trimming A by 20 20 70 20, reference by 20 20 65 20 1.2 3.2 unbinned pixels; 10.4 9.1 nm along two shift axes; -12.8 3.8 nm on specimen axes Total_Shift = 6.878989 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 15.44, -7.88 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -8.9, 5.8 unbinned pixels, 0.19 nm/sec Measured defocus = -0.53 microns changed by -1.07 to target 34 frames are being saved to U:\krios\20190806\slot11_0017.tif 34 frames are being saved to U:\krios\20190806\slot11_0018.tif 34 frames are being saved to U:\krios\20190806\slot11_0019.tif Item 69: Stage: -765.78 631.79 70 Label: 67-1 69 Tick time from program start = 26305.060 Running script 17 at item 68-1 (-764.78, 629.49) Remaining time to fill dewars is 30540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 90 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.27 -0.19 (2nd move error 0.18 0.01) Finished aligning to item in 1 rounds Trimming A by 20 20 95 20, reference by 20 20 90 20 1.6 -7.1 unbinned pixels; 28.3 4.8 nm along two shift axes; 27.3 7.9 nm on specimen axes Total_Shift = 14.592896 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 18.54, -10.44 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -6.4, 11.0 unbinned pixels, 0.23 nm/sec Measured defocus = -0.66 microns changed by -1.04 to target 34 frames are being saved to U:\krios\20190806\slot11_0020.tif 34 frames are being saved to U:\krios\20190806\slot11_0021.tif 34 frames are being saved to U:\krios\20190806\slot11_0022.tif Item 70: Stage: -764.78 629.49 70 Label: 68-1 70 Tick time from program start = 26416.195 Running script 17 at item 69-1 (-764.60, 623.64) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.23 -0.22 Trimming A by 20 20 85 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.21 -0.09 (2nd move error -0.03 0.12) Finished aligning to item in 2 rounds Trimming A by 20 20 85 20, reference by 20 20 85 20 3.2 7.7 unbinned pixels; 24.2 23.1 nm along two shift axes; -30.6 10.8 nm on specimen axes Total_Shift = 16.680737 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 25.44, -12.60 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -8.0, 5.5 unbinned pixels, 0.17 nm/sec Measured defocus = -0.87 microns changed by -0.93 to target 34 frames are being saved to U:\krios\20190806\slot11_0023.tif 34 frames are being saved to U:\krios\20190806\slot11_0024.tif 34 frames are being saved to U:\krios\20190806\slot11_0025.tif Item 71: Stage: -764.60 623.64 70 Label: 69-1 71 Tick time from program start = 26534.772 Running script 17 at item 70 (-766.97, 628.40) Remaining time to fill dewars is 30300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.38 -0.22 (2nd move error 0.29 -0.02) Finished aligning to item in 1 rounds Trimming A by 20 20 25 20, reference by 20 20 20 20 -1.3 -1.8 unbinned pixels; 4.9 7.5 nm along two shift axes; 7.3 -4.8 nm on specimen axes Total_Shift = 4.479252 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 22.29, -13.12 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -0.6, 8.0 unbinned pixels, 0.14 nm/sec Measured defocus = -0.80 microns changed by -1.10 to target 34 frames are being saved to U:\krios\20190806\slot11_0026.tif Ending Navigator acquisition after current item 34 frames are being saved to U:\krios\20190806\slot11_0027.tif 34 frames are being saved to U:\krios\20190806\slot11_0028.tif Item 72: Stage: -766.97 628.40 70 Label: 70 72 Tick time from program start = 26645.704 IlluminatedArea 0.008797 IlluminatedArea 0.008799 Running script 17 at item 71 (-768.03, 630.71) Remaining time to fill dewars is 30240 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.23 -0.26 Disparity in stage position after aligning to target position: 0.28 -0.20 (2nd move error 0.04 0.04) Finished aligning to item in 2 rounds -0.1 -0.4 unbinned pixels; 1.4 0.8 nm along two shift axes; 1.5 -0.1 nm on specimen axes Total_Shift = 0.794244 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 27.13, -15.84 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -8.0, 9.0 unbinned pixels, 0.21 nm/sec Measured defocus = -0.97 microns changed by -1.03 to target 34 frames are being saved to U:\krios\20190806\slot11_0029.tif 34 frames are being saved to U:\krios\20190806\slot11_0030.tif 34 frames are being saved to U:\krios\20190806\slot11_0031.tif Item 73: Stage: -768.03 630.71 70 Label: 71 73 Tick time from program start = 26862.483 Running script 17 at item 72 (-769.07, 633.04) Remaining time to fill dewars is 30120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.27 -0.23 (2nd move error 0.04 0.01) Finished aligning to item in 1 rounds 1.6 -1.6 unbinned pixels; 8.0 3.3 nm along two shift axes; 5.9 6.5 nm on specimen axes Total_Shift = 4.500656 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 36.86, -21.63 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -9.0, 11.9 unbinned pixels, 0.26 nm/sec Measured defocus = -1.32 microns changed by -0.78 to target 34 frames are being saved to U:\krios\20190806\slot11_0032.tif 34 frames are being saved to U:\krios\20190806\slot11_0033.tif 34 frames are being saved to U:\krios\20190806\slot11_0034.tif Item 74: Stage: -769.07 633.04 70 Label: 72 74 Tick time from program start = 26976.395 Running script 17 at item 73 (-770.15, 635.38) Remaining time to fill dewars is 30000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.29 -0.26 (2nd move error 0.05 -0.01) Finished aligning to item in 1 rounds -2.9 -0.8 unbinned pixels; 0.9 11.7 nm along two shift axes; 3.7 -11.2 nm on specimen axes Total_Shift = 6.033779 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 42.85, -26.11 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -4.5, 8.4 unbinned pixels, 0.17 nm/sec Measured defocus = -1.55 microns changed by -0.65 to target 34 frames are being saved to U:\krios\20190806\slot11_0035.tif 34 frames are being saved to U:\krios\20190806\slot11_0036.tif 34 frames are being saved to U:\krios\20190806\slot11_0037.tif Item 75: Stage: -770.15 635.38 70 Label: 73 75 Tick time from program start = 27087.857 Running script 17 at item 74 (-771.21, 637.72) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.20 -0.29 (2nd move error -0.04 -0.04) Finished aligning to item in 1 rounds -6.0 8.4 unbinned pixels; 38.6 8.9 nm along two shift axes; -31.3 -25.0 nm on specimen axes Total_Shift = 20.567581 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 46.87, -29.55 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -3.7, 4.2 unbinned pixels, 0.10 nm/sec Measured defocus = -1.71 microns changed by -0.59 to target 34 frames are being saved to U:\krios\20190806\slot11_0038.tif 34 frames are being saved to U:\krios\20190806\slot11_0039.tif 34 frames are being saved to U:\krios\20190806\slot11_0040.tif Item 76: Stage: -771.21 637.72 70 Label: 74 76 Tick time from program start = 27199.273 Running script 17 at item 75 (-772.52, 640.00) Remaining time to fill dewars is 29820 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.34 -0.20 Disparity in stage position after aligning to target position: 0.39 -0.29 (2nd move error 0.04 -0.11) Finished aligning to item in 2 rounds -13.8 -3.3 unbinned pixels; 5.8 54.6 nm along two shift axes; 15.8 -52.9 nm on specimen axes Total_Shift = 28.349604 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 51.40, -31.93 unbinned pixels, unfiltered CCC is -0.0014 The average drift between shots was 2.5, 10.0 unbinned pixels, 0.18 nm/sec Measured defocus = -1.87 microns changed by -0.53 to target 34 frames are being saved to U:\krios\20190806\slot11_0041.tif 34 frames are being saved to U:\krios\20190806\slot11_0042.tif 34 frames are being saved to U:\krios\20190806\slot11_0043.tif Item 77: Stage: -772.52 640.00 70 Label: 75 77 Tick time from program start = 27325.447 Running script 17 at item 76 (-776.78, 649.39) Remaining time to fill dewars is 29820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 45 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.17 -0.26 (2nd move error -0.18 -0.08) Finished aligning to item in 1 rounds Trimming A by 45 20 20 20, reference by 45 20 20 20 -10.1 33.3 unbinned pixels; 135.5 13.8 nm along two shift axes; -127.3 -46.2 nm on specimen axes Total_Shift = 69.530419 Trimming A by 45 20 20 20, reference by 45 20 20 20 -5.9 22.4 unbinned pixels; 89.9 12.4 nm along two shift axes; -85.7 -27.7 nm on specimen axes Total_Shift = 46.240781 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 44.99, -26.86 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -2.6, 0.5 unbinned pixels, 0.05 nm/sec Measured defocus = -1.62 microns changed by -0.88 to target 34 frames are being saved to U:\krios\20190806\slot11_0044.tif 34 frames are being saved to U:\krios\20190806\slot11_0045.tif 34 frames are being saved to U:\krios\20190806\slot11_0046.tif Item 78: Stage: -776.78 649.39 70 Label: 76 78 Tick time from program start = 27442.916 Running script 17 at item 77 (-779.12, 648.25) Remaining time to fill dewars is 29580 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.13 -0.32 Disparity in stage position after aligning to target position: 0.31 -0.22 (2nd move error 0.18 0.07) Finished aligning to item in 2 rounds -0.6 2.8 unbinned pixels; 11.1 1.9 nm along two shift axes; -10.8 -3.1 nm on specimen axes Total_Shift = 5.750272 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 50.08, -31.62 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -0.4, 2.6 unbinned pixels, 0.05 nm/sec Measured defocus = -1.83 microns changed by 0.53 to target 34 frames are being saved to U:\krios\20190806\slot11_0047.tif 34 frames are being saved to U:\krios\20190806\slot11_0048.tif 34 frames are being saved to U:\krios\20190806\slot11_0049.tif Item 79: Stage: -779.12 648.25 70 Label: 77 79 Tick time from program start = 27562.116 Running script 17 at item 78 (-773.51, 636.69) Remaining time to fill dewars is 29520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.10 -0.29 (2nd move error -0.13 -0.05) Finished aligning to item in 1 rounds -6.4 24.5 unbinned pixels; 98.4 13.9 nm along two shift axes; -94.0 -30.0 nm on specimen axes Total_Shift = 50.636158 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 18.42, -11.12 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -7.3, 4.7 unbinned pixels, 0.16 nm/sec Measured defocus = -0.67 microns changed by -0.63 to target 34 frames are being saved to U:\krios\20190806\slot11_0050.tif 34 frames are being saved to U:\krios\20190806\slot11_0051.tif 34 frames are being saved to U:\krios\20190806\slot11_0052.tif Item 80: Stage: -773.51 636.69 70 Label: 78 80 Tick time from program start = 27673.875 Running script 17 at item 79 (-772.43, 634.29) Remaining time to fill dewars is 29520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.18 -0.32 (2nd move error -0.05 -0.07) Finished aligning to item in 1 rounds -9.2 12.0 unbinned pixels; 56.0 15.2 nm along two shift axes; -44.6 -38.4 nm on specimen axes Total_Shift = 30.20395 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 18.45, -11.97 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -7.6, 3.8 unbinned pixels, 0.15 nm/sec Measured defocus = -0.68 microns changed by -0.72 to target 34 frames are being saved to U:\krios\20190806\slot11_0053.tif 34 frames are being saved to U:\krios\20190806\slot11_0054.tif 34 frames are being saved to U:\krios\20190806\slot11_0055.tif Item 81: Stage: -772.43 634.29 70 Label: 79 81 Tick time from program start = 27784.823 Running script 17 at item 80 (-771.35, 631.92) Remaining time to fill dewars is 29400 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.23 -0.28 Aligning to target with image shift equivalent to stage shift: -0.61 -0.06 Finished aligning to item in 1 rounds -21.3 119.0 unbinned pixels; 465.4 102.5 nm along two shift axes; -458.5 -107.9 nm on specimen axes Total_Shift = 241.813902 -17.2 64.0 unbinned pixels; 258.0 34.3 nm along two shift axes; -245.5 -80.6 nm on specimen axes Total_Shift = 132.653647 Total_shift never < 120: Skiping ................................................ Running script 17 at item 81 (-770.24, 629.61) Remaining time to fill dewars is 29280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.34 -0.31 (2nd move error 0.10 -0.05) Finished aligning to item in 1 rounds -5.9 -1.0 unbinned pixels; 4.2 22.7 nm along two shift axes; 5.0 -22.7 nm on specimen axes Total_Shift = 11.946978 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 15.42, -9.58 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -4.8, 9.1 unbinned pixels, 0.18 nm/sec Measured defocus = -0.56 microns changed by -0.94 to target 34 frames are being saved to U:\krios\20190806\slot11_0056.tif 34 frames are being saved to U:\krios\20190806\slot11_0057.tif 34 frames are being saved to U:\krios\20190806\slot11_0058.tif Item 83: Stage: -770.24 629.61 70 Label: 81 83 Tick time from program start = 27926.207 Running script 17 at item 82 (-769.20, 627.30) Remaining time to fill dewars is 29280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.31 -0.27 (2nd move error 0.07 -0.02) Finished aligning to item in 1 rounds -2.8 0.2 unbinned pixels; 4.5 9.7 nm along two shift axes; -0.3 -10.9 nm on specimen axes Total_Shift = 5.584892 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 13.68, -9.44 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -3.4, 8.5 unbinned pixels, 0.16 nm/sec Measured defocus = -0.51 microns changed by -1.09 to target 34 frames are being saved to U:\krios\20190806\slot11_0059.tif 34 frames are being saved to U:\krios\20190806\slot11_0060.tif 34 frames are being saved to U:\krios\20190806\slot11_0061.tif Item 84: Stage: -769.20 627.30 70 Label: 82 84 Tick time from program start = 28037.046 Running script 17 at item 83 (-767.94, 624.91) Remaining time to fill dewars is 29160 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.29 -0.27 Disparity in stage position after aligning to target position: 0.14 -0.21 (2nd move error -0.16 0.04) Finished aligning to item in 2 rounds 0.3 31.3 unbinned pixels; 114.8 48.2 nm along two shift axes; -121.9 -5.4 nm on specimen axes Total_Shift = 62.657468 -2.8 20.5 unbinned pixels; 78.9 20.9 nm along two shift axes; -79.1 -15.0 nm on specimen axes Total_Shift = 41.320557 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 19.27, -10.89 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -3.2, 2.6 unbinned pixels, 0.07 nm/sec Measured defocus = -0.68 microns changed by -1.02 to target 34 frames are being saved to U:\krios\20190806\slot11_0062.tif 34 frames are being saved to U:\krios\20190806\slot11_0063.tif 34 frames are being saved to U:\krios\20190806\slot11_0064.tif Item 85: Stage: -767.94 624.91 70 Label: 83 85 Tick time from program start = 28159.818 Running script 17 at item 84 (-766.85, 622.56) Remaining time to fill dewars is 29100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.26 -0.18 (2nd move error -0.03 0.07) Finished aligning to item in 1 rounds 0.9 6.5 unbinned pixels; 22.7 13.1 nm along two shift axes; -25.4 2.2 nm on specimen axes Total_Shift = 13.105301 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 23.19, -12.11 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -9.2, 3.9 unbinned pixels, 0.18 nm/sec Measured defocus = -0.81 microns changed by -0.99 to target 34 frames are being saved to U:\krios\20190806\slot11_0065.tif 34 frames are being saved to U:\krios\20190806\slot11_0066.tif 34 frames are being saved to U:\krios\20190806\slot11_0067.tif Item 86: Stage: -766.85 622.56 70 Label: 84 86 Tick time from program start = 28270.938 Running script 17 at item 85 (-765.80, 620.23) Remaining time to fill dewars is 28980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 40 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.23 -0.16 (2nd move error -0.06 0.09) Finished aligning to item in 1 rounds Trimming A by 20 20 40 20, reference by 20 20 40 20 -1.4 13.6 unbinned pixels; 51.8 15.3 nm along two shift axes; -52.5 -8.4 nm on specimen axes Total_Shift = 27.303719 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 23.56, -12.14 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -6.4, 1.8 unbinned pixels, 0.12 nm/sec Measured defocus = -0.82 microns changed by -1.08 to target 34 frames are being saved to U:\krios\20190806\slot11_0068.tif 34 frames are being saved to U:\krios\20190806\slot11_0069.tif 34 frames are being saved to U:\krios\20190806\slot11_0070.tif Item 87: Stage: -765.80 620.23 70 Label: 85 87 Tick time from program start = 28382.120 Running script 17 at item 86 (-764.74, 617.92) Remaining time to fill dewars is 28860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 70 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.32 -0.14 (2nd move error 0.03 0.11) Finished aligning to item in 1 rounds Trimming A by 20 20 70 20, reference by 20 20 70 20 -0.3 -1.3 unbinned pixels; 4.4 3.1 nm along two shift axes; 5.1 -1.0 nm on specimen axes Total_Shift = 2.673901 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 28.70, -18.82 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -1.3, 8.8 unbinned pixels, 0.16 nm/sec Measured defocus = -1.06 microns changed by -0.94 to target 34 frames are being saved to U:\krios\20190806\slot11_0071.tif 34 frames are being saved to U:\krios\20190806\slot11_0072.tif 34 frames are being saved to U:\krios\20190806\slot11_0073.tif Item 88: Stage: -764.74 617.92 70 Label: 86 88 Tick time from program start = 28495.782 Running script 17 at item 87 (-765.95, 614.56) Remaining time to fill dewars is 28860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 30 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.36 -0.22 (2nd move error 0.07 0.03) Finished aligning to item in 1 rounds Trimming A by 20 25 30 20, reference by 20 20 30 20 1.3 1.6 unbinned pixels; 4.1 7.0 nm along two shift axes; -6.3 4.7 nm on specimen axes Total_Shift = 4.043091 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 36.16, -22.41 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -6.3, 7.5 unbinned pixels, 0.17 nm/sec Measured defocus = -1.32 microns changed by -0.78 to target 34 frames are being saved to U:\krios\20190806\slot11_0074.tif 34 frames are being saved to U:\krios\20190806\slot11_0075.tif 34 frames are being saved to U:\krios\20190806\slot11_0076.tif Item 89: Stage: -765.95 614.56 70 Label: 87 89 Tick time from program start = 28610.100 Running script 17 at item 88 (-766.97, 616.84) Remaining time to fill dewars is 28800 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.39 -0.22 (2nd move error 0.10 0.03) Finished aligning to item in 1 rounds 0.2 -0.8 unbinned pixels; 3.0 0.6 nm along two shift axes; 2.9 0.7 nm on specimen axes Total_Shift = 1.550089 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 38.97, -22.69 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -2.5, 7.6 unbinned pixels, 0.14 nm/sec Measured defocus = -1.39 microns changed by -0.81 to target 34 frames are being saved to U:\krios\20190806\slot11_0077.tif 34 frames are being saved to U:\krios\20190806\slot11_0078.tif 34 frames are being saved to U:\krios\20190806\slot11_0079.tif Item 90: Stage: -766.97 616.84 70 Label: 88 90 Tick time from program start = 28740.813 Running script 17 at item 89 (-768.03, 619.15) Remaining time to fill dewars is 28680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.26 -0.19 (2nd move error -0.03 0.06) Finished aligning to item in 1 rounds -6.1 7.6 unbinned pixels; 35.8 10.5 nm along two shift axes; -28.1 -25.3 nm on specimen axes Total_Shift = 19.418318 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 36.64, -22.17 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was 1.5, 1.4 unbinned pixels, 0.04 nm/sec Measured defocus = -1.32 microns changed by -0.98 to target 34 frames are being saved to U:\krios\20190806\slot11_0080.tif 34 frames are being saved to U:\krios\20190806\slot11_0081.tif 34 frames are being saved to U:\krios\20190806\slot11_0082.tif Item 91: Stage: -768.03 619.15 70 Label: 89 91 Tick time from program start = 28855.692 Running script 17 at item 90 (-769.12, 621.52) Remaining time to fill dewars is 28560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.22 -0.22 (2nd move error -0.07 0.03) Finished aligning to item in 1 rounds -0.2 11.9 unbinned pixels; 44.0 17.1 nm along two shift axes; -46.2 -3.4 nm on specimen axes Total_Shift = 23.801294 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 71.61, -45.98 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -5.3, 5.5 unbinned pixels, 0.13 nm/sec Measured defocus = -2.63 microns changed by 0.23 to target 34 frames are being saved to U:\krios\20190806\slot11_0083.tif 34 frames are being saved to U:\krios\20190806\slot11_0084.tif 34 frames are being saved to U:\krios\20190806\slot11_0085.tif Item 92: Stage: -769.12 621.52 70 Label: 90 92 Tick time from program start = 28966.936 Running script 17 at item 91 (-770.20, 623.86) Remaining time to fill dewars is 28380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.26 -0.21 (2nd move error -0.03 0.04) Finished aligning to item in 1 rounds -6.6 13.5 unbinned pixels; 58.3 3.3 nm along two shift axes; -51.2 -28.4 nm on specimen axes Total_Shift = 30.057706 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 39.13, -26.08 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -0.1, 2.7 unbinned pixels, 0.05 nm/sec Measured defocus = -1.45 microns changed by -1.05 to target 34 frames are being saved to U:\krios\20190806\slot11_0086.tif 34 frames are being saved to U:\krios\20190806\slot11_0087.tif 34 frames are being saved to U:\krios\20190806\slot11_0088.tif Item 93: Stage: -770.20 623.86 70 Label: 91 93 Tick time from program start = 29078.867 Running script 17 at item 92 (-771.46, 626.23) Remaining time to fill dewars is 28380 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.29 -0.30 Disparity in stage position after aligning to target position: 0.38 -0.32 (2nd move error 0.09 -0.04) Finished aligning to item in 2 rounds -3.6 0.2 unbinned pixels; 5.4 12.8 nm along two shift axes; 0.1 -14.2 nm on specimen axes Total_Shift = 7.28855 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 40.27, -26.63 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was 0.1, 6.8 unbinned pixels, 0.12 nm/sec Measured defocus = -1.49 microns changed by 0.19 to target 34 frames are being saved to U:\krios\20190806\slot11_0089.tif 34 frames are being saved to U:\krios\20190806\slot11_0090.tif 34 frames are being saved to U:\krios\20190806\slot11_0091.tif Item 94: Stage: -771.46 626.23 70 Label: 92 94 Tick time from program start = 29196.336 Running script 17 at item 93 (-772.52, 628.54) Remaining time to fill dewars is 28260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.29 -0.32 (2nd move error 0.00 -0.04) Finished aligning to item in 1 rounds -6.5 0.4 unbinned pixels; 9.9 22.7 nm along two shift axes; -0.1 -25.3 nm on specimen axes Total_Shift = 12.985462 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 10.18, -6.52 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -2.4, 4.7 unbinned pixels, 0.09 nm/sec Measured defocus = -0.37 microns changed by -1.03 to target 34 frames are being saved to U:\krios\20190806\slot11_0092.tif 34 frames are being saved to U:\krios\20190806\slot11_0093.tif 34 frames are being saved to U:\krios\20190806\slot11_0094.tif Item 95: Stage: -772.52 628.54 70 Label: 93 95 Tick time from program start = 29307.502 Running script 17 at item 94 (-773.62, 630.89) Remaining time to fill dewars is 28140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.33 (2nd move error -0.06 -0.05) Finished aligning to item in 1 rounds -6.3 12.7 unbinned pixels; 54.9 3.8 nm along two shift axes; -48.0 -27.3 nm on specimen axes Total_Shift = 28.352178 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 15.80, -6.26 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -5.0, 1.0 unbinned pixels, 0.09 nm/sec Measured defocus = -0.52 microns changed by -0.98 to target 34 frames are being saved to U:\krios\20190806\slot11_0095.tif 34 frames are being saved to U:\krios\20190806\slot11_0096.tif 34 frames are being saved to U:\krios\20190806\slot11_0097.tif Item 96: Stage: -773.62 630.89 70 Label: 94 96 Tick time from program start = 29418.778 Running script 17 at item 95 (-774.68, 633.26) Remaining time to fill dewars is 28020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.27 -0.36 (2nd move error -0.03 -0.08) Finished aligning to item in 1 rounds -9.0 7.5 unbinned pixels; 39.2 21.3 nm along two shift axes; -27.1 -36.7 nm on specimen axes Total_Shift = 23.43829 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 21.24, -14.49 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -6.1, 4.3 unbinned pixels, 0.13 nm/sec Measured defocus = -0.79 microns changed by -0.81 to target 34 frames are being saved to U:\krios\20190806\slot11_0098.tif 34 frames are being saved to U:\krios\20190806\slot11_0099.tif 34 frames are being saved to U:\krios\20190806\slot11_0100.tif Item 97: Stage: -774.68 633.26 70 Label: 95 97 Tick time from program start = 29530.396 Running script 17 at item 96 (-775.77, 635.61) Remaining time to fill dewars is 28020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.16 -0.38 (2nd move error -0.14 -0.10) Finished aligning to item in 1 rounds -13.6 26.0 unbinned pixels; 113.3 9.8 nm along two shift axes; -98.2 -58.3 nm on specimen axes Total_Shift = 58.632882 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 28.17, -16.48 unbinned pixels, unfiltered CCC is -0.0013 The average drift between shots was -6.9, 2.9 unbinned pixels, 0.13 nm/sec Measured defocus = -1.01 microns changed by -0.69 to target 34 frames are being saved to U:\krios\20190806\slot11_0101.tif 34 frames are being saved to U:\krios\20190806\slot11_0102.tif 34 frames are being saved to U:\krios\20190806\slot11_0103.tif Item 98: Stage: -775.77 635.61 70 Label: 96 98 Tick time from program start = 29642.358 Running script 17 at item 97 (-776.75, 637.97) Remaining time to fill dewars is 27840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.14 -0.40 (2nd move error -0.16 -0.12) Finished aligning to item in 1 rounds -16.6 29.7 unbinned pixels; 130.7 14.9 nm along two shift axes; -111.9 -70.6 nm on specimen axes Total_Shift = 67.94372 -8.7 19.8 unbinned pixels; 84.1 1.5 nm along two shift axes; -75.2 -38.0 nm on specimen axes Total_Shift = 43.244603 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 32.49, -19.63 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -4.2, 4.7 unbinned pixels, 0.11 nm/sec Measured defocus = -1.17 microns changed by -0.63 to target 34 frames are being saved to U:\krios\20190806\slot11_0104.tif 34 frames are being saved to U:\krios\20190806\slot11_0105.tif 34 frames are being saved to U:\krios\20190806\slot11_0106.tif Item 99: Stage: -776.75 637.97 70 Label: 97 99 Tick time from program start = 29759.889 Running script 17 at item 98 (-778.14, 640.25) Remaining time to fill dewars is 27720 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.10 -0.35 Disparity in stage position after aligning to target position: 0.43 -0.30 (2nd move error 0.32 0.03) Finished aligning to item in 2 rounds 2.1 -2.5 unbinned pixels; 12.1 3.9 nm along two shift axes; 9.4 8.8 nm on specimen axes Total_Shift = 6.639124 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 37.04, -23.48 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -5.8, 11.7 unbinned pixels, 0.23 nm/sec Measured defocus = -1.36 microns changed by -0.54 to target 34 frames are being saved to U:\krios\20190806\slot11_0107.tif 34 frames are being saved to U:\krios\20190806\slot11_0108.tif 34 frames are being saved to U:\krios\20190806\slot11_0109.tif Item 100: Stage: -778.14 640.25 70 Label: 98 100 Tick time from program start = 29880.790 Running script 17 at item 99 (-780.26, 644.84) Remaining time to fill dewars is 27720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.36 -0.30 (2nd move error 0.26 0.03) Finished aligning to item in 1 rounds 1.6 -4.2 unbinned pixels; 17.7 0.5 nm along two shift axes; 16.1 7.3 nm on specimen axes Total_Shift = 9.087495 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 38.99, -24.01 unbinned pixels, unfiltered CCC is -0.0013 The average drift between shots was -6.6, 10.2 unbinned pixels, 0.22 nm/sec Measured defocus = -1.42 microns changed by -0.58 to target 34 frames are being saved to U:\krios\20190806\slot11_0110.tif 34 frames are being saved to U:\krios\20190806\slot11_0111.tif 34 frames are being saved to U:\krios\20190806\slot11_0112.tif Item 101: Stage: -780.26 644.84 70 Label: 99 101 Tick time from program start = 29992.456 Running script 17 at item 100 (-781.31, 647.18) Remaining time to fill dewars is 27540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.25 -0.33 (2nd move error 0.15 -0.00) Finished aligning to item in 1 rounds -0.9 1.7 unbinned pixels; 7.6 0.7 nm along two shift axes; -6.6 -4.0 nm on specimen axes Total_Shift = 3.953242 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 39.21, -25.57 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -1.8, 8.8 unbinned pixels, 0.16 nm/sec Measured defocus = -1.45 microns changed by -0.65 to target 34 frames are being saved to U:\krios\20190806\slot11_0113.tif 34 frames are being saved to U:\krios\20190806\slot11_0114.tif 34 frames are being saved to U:\krios\20190806\slot11_0115.tif Item 102: Stage: -781.31 647.18 70 Label: 100 102 Tick time from program start = 30112.686 Running script 17 at item 101 (-782.38, 649.58) Remaining time to fill dewars is 27420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 65 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.29 -0.33 (2nd move error 0.19 0.01) Finished aligning to item in 1 rounds Trimming A by 65 20 20 20, reference by 65 20 20 20 1.3 -4.8 unbinned pixels; 19.4 2.6 nm along two shift axes; 18.4 6.0 nm on specimen axes Total_Shift = 9.962557 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 35.73, -21.88 unbinned pixels, unfiltered CCC is 0.0071 The average drift between shots was -0.3, 11.7 unbinned pixels, 0.21 nm/sec Measured defocus = -1.30 microns changed by -0.90 to target 34 frames are being saved to U:\krios\20190806\slot11_0116.tif 34 frames are being saved to U:\krios\20190806\slot11_0117.tif 34 frames are being saved to U:\krios\20190806\slot11_0118.tif Item 103: Stage: -782.38 649.58 70 Label: 101 103 Tick time from program start = 30227.814 Running script 17 at item 102 (-784.61, 648.48) Remaining time to fill dewars is 27300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.17 -0.36 (2nd move error 0.06 -0.03) Finished aligning to item in 1 rounds Trimming A by 35 20 20 20, reference by 35 20 20 20 -3.8 0.8 unbinned pixels; 7.7 12.5 nm along two shift axes; -2.2 -14.9 nm on specimen axes Total_Shift = 7.715002 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 39.17, -27.38 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was 3.3, 9.2 unbinned pixels, 0.17 nm/sec Measured defocus = -1.47 microns changed by -0.83 to target 34 frames are being saved to U:\krios\20190806\slot11_0119.tif 34 frames are being saved to U:\krios\20190806\slot11_0120.tif 34 frames are being saved to U:\krios\20190806\slot11_0121.tif Item 104: Stage: -784.61 648.48 70 Label: 102 104 Tick time from program start = 30339.433 Running script 17 at item 103 (-783.62, 646.11) Remaining time to fill dewars is 27300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.25 -0.35 (2nd move error 0.14 -0.01) Finished aligning to item in 1 rounds -2.0 -6.2 unbinned pixels; 20.0 16.6 nm along two shift axes; 24.4 -6.6 nm on specimen axes Total_Shift = 13.002521 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 47.11, -31.10 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was 1.4, 12.6 unbinned pixels, 0.22 nm/sec Measured defocus = -1.74 microns changed by -0.66 to target 34 frames are being saved to U:\krios\20190806\slot11_0122.tif 34 frames are being saved to U:\krios\20190806\slot11_0123.tif 34 frames are being saved to U:\krios\20190806\slot11_0124.tif Item 105: Stage: -783.62 646.11 70 Label: 103 105 Tick time from program start = 30450.755 Running script 17 at item 104 (-781.47, 641.47) Remaining time to fill dewars is 27240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.39 -0.35 (2nd move error 0.28 -0.02) Finished aligning to item in 1 rounds 0.2 8.1 unbinned pixels; 29.6 12.8 nm along two shift axes; -31.5 -1.0 nm on specimen axes Total_Shift = 16.201903 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 55.08, -32.93 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -3.7, 5.0 unbinned pixels, 0.11 nm/sec Measured defocus = -1.98 microns changed by -0.52 to target 34 frames are being saved to U:\krios\20190806\slot11_0125.tif 34 frames are being saved to U:\krios\20190806\slot11_0126.tif 34 frames are being saved to U:\krios\20190806\slot11_0127.tif Item 106: Stage: -781.47 641.47 70 Label: 104 106 Tick time from program start = 30562.561 Running script 17 at item 105 (-780.48, 639.14) Remaining time to fill dewars is 27120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.46 -0.37 (2nd move error 0.35 -0.04) Finished aligning to item in 1 rounds -1.2 -5.7 unbinned pixels; 19.4 12.8 nm along two shift axes; 22.4 -3.4 nm on specimen axes Total_Shift = 11.633323 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 18.89, -9.95 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -2.6, 10.3 unbinned pixels, 0.19 nm/sec Measured defocus = -0.66 microns changed by -0.64 to target 34 frames are being saved to U:\krios\20190806\slot11_0128.tif 34 frames are being saved to U:\krios\20190806\slot11_0129.tif 34 frames are being saved to U:\krios\20190806\slot11_0130.tif Item 107: Stage: -780.48 639.14 70 Label: 105 107 Tick time from program start = 30673.821 Running script 17 at item 106 (-779.15, 636.64) Remaining time to fill dewars is 27000 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.29 -0.29 Disparity in stage position after aligning to target position: 0.30 -0.13 (2nd move error 0.00 0.14) Finished aligning to item in 2 rounds 1.0 0.8 unbinned pixels; 1.5 4.9 nm along two shift axes; -3.2 3.8 nm on specimen axes Total_Shift = 2.570703 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 16.91, -9.23 unbinned pixels, unfiltered CCC is -0.0010 The average drift between shots was -9.4, 6.0 unbinned pixels, 0.19 nm/sec Measured defocus = -0.59 microns changed by -0.71 to target 34 frames are being saved to U:\krios\20190806\slot11_0131.tif 34 frames are being saved to U:\krios\20190806\slot11_0132.tif 34 frames are being saved to U:\krios\20190806\slot11_0133.tif Item 108: Stage: -779.15 636.64 70 Label: 106 108 Tick time from program start = 30791.914 Running script 17 at item 107 (-778.10, 634.30) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.33 -0.16 (2nd move error 0.03 0.11) Finished aligning to item in 1 rounds -0.5 -1.2 unbinned pixels; 3.8 3.7 nm along two shift axes; 4.9 -1.7 nm on specimen axes Total_Shift = 2.648133 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 20.03, -12.19 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -9.8, 9.7 unbinned pixels, 0.25 nm/sec Measured defocus = -0.72 microns changed by -0.68 to target 34 frames are being saved to U:\krios\20190806\slot11_0134.tif 34 frames are being saved to U:\krios\20190806\slot11_0135.tif 34 frames are being saved to U:\krios\20190806\slot11_0136.tif Item 109: Stage: -778.10 634.30 70 Label: 107 109 Tick time from program start = 30902.675 Running script 17 at item 108 (-777.09, 631.98) Remaining time to fill dewars is 26820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.37 -0.13 (2nd move error 0.07 0.15) Finished aligning to item in 1 rounds 1.3 1.8 unbinned pixels; 4.7 7.4 nm along two shift axes; -7.1 4.8 nm on specimen axes Total_Shift = 4.402946 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 19.53, -12.41 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -8.9, 8.8 unbinned pixels, 0.22 nm/sec Measured defocus = -0.72 microns changed by -0.78 to target 34 frames are being saved to U:\krios\20190806\slot11_0137.tif 34 frames are being saved to U:\krios\20190806\slot11_0138.tif 34 frames are being saved to U:\krios\20190806\slot11_0139.tif Item 110: Stage: -777.09 631.98 70 Label: 108 110 Tick time from program start = 31013.170 Running script 17 at item 109 (-775.85, 629.85) Remaining time to fill dewars is 26700 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.16 -0.23 Disparity in stage position after aligning to target position: 0.16 -0.37 (2nd move error -0.00 -0.16) Finished aligning to item in 2 rounds -21.6 3.6 unbinned pixels; 41.4 72.4 nm along two shift axes; -9.4 -84.8 nm on specimen axes Total_Shift = 43.791862 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 19.66, -12.16 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -1.4, 4.3 unbinned pixels, 0.08 nm/sec Measured defocus = -0.71 microns changed by -0.89 to target 34 frames are being saved to U:\krios\20190806\slot11_0140.tif 34 frames are being saved to U:\krios\20190806\slot11_0141.tif 34 frames are being saved to U:\krios\20190806\slot11_0142.tif Item 111: Stage: -775.85 629.85 70 Label: 109 111 Tick time from program start = 31132.215 Running script 17 at item 110 (-774.75, 627.54) Remaining time to fill dewars is 26580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.29 -0.35 (2nd move error 0.13 -0.14) Finished aligning to item in 1 rounds -16.8 -4.0 unbinned pixels; 7.2 66.7 nm along two shift axes; 19.2 -64.6 nm on specimen axes Total_Shift = 34.612303 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 21.78, -12.95 unbinned pixels, unfiltered CCC is -0.0013 The average drift between shots was -0.9, 8.7 unbinned pixels, 0.15 nm/sec Measured defocus = -0.78 microns changed by -0.92 to target 34 frames are being saved to U:\krios\20190806\slot11_0143.tif 34 frames are being saved to U:\krios\20190806\slot11_0144.tif 34 frames are being saved to U:\krios\20190806\slot11_0145.tif Item 112: Stage: -774.75 627.54 70 Label: 110 112 Tick time from program start = 31244.052 Running script 17 at item 111 (-773.69, 625.25) Remaining time to fill dewars is 26520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.29 -0.36 (2nd move error 0.13 -0.15) Finished aligning to item in 1 rounds -15.9 1.8 unbinned pixels; 27.5 54.5 nm along two shift axes; -3.8 -62.3 nm on specimen axes Total_Shift = 32.030437 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 24.43, -15.71 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -0.9, 7.5 unbinned pixels, 0.14 nm/sec Measured defocus = -0.90 microns changed by -0.90 to target 34 frames are being saved to U:\krios\20190806\slot11_0146.tif 34 frames are being saved to U:\krios\20190806\slot11_0147.tif 34 frames are being saved to U:\krios\20190806\slot11_0148.tif Item 113: Stage: -773.69 625.25 70 Label: 111 113 Tick time from program start = 31355.358 Running script 17 at item 112 (-772.43, 622.84) Remaining time to fill dewars is 26400 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.20 -0.22 Disparity in stage position after aligning to target position: 0.11 -0.25 (2nd move error -0.10 -0.05) Finished aligning to item in 2 rounds -7.7 19.8 unbinned pixels; 82.8 1.9 nm along two shift axes; -75.3 -34.3 nm on specimen axes Total_Shift = 42.46073 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 25.77, -16.19 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -3.5, 1.7 unbinned pixels, 0.07 nm/sec Measured defocus = -0.94 microns changed by -0.96 to target 34 frames are being saved to U:\krios\20190806\slot11_0149.tif 34 frames are being saved to U:\krios\20190806\slot11_0150.tif 34 frames are being saved to U:\krios\20190806\slot11_0151.tif Item 114: Stage: -772.43 622.84 70 Label: 112 114 Tick time from program start = 31478.053 Running script 17 at item 113 (-771.35, 620.45) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.28 (2nd move error -0.02 -0.08) Finished aligning to item in 1 rounds -13.4 11.7 unbinned pixels; 60.6 30.7 nm along two shift axes; -42.8 -54.7 nm on specimen axes Total_Shift = 35.633998 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 30.19, -18.42 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was 0.3, 1.3 unbinned pixels, 0.02 nm/sec Measured defocus = -1.09 microns changed by -0.91 to target 34 frames are being saved to U:\krios\20190806\slot11_0152.tif 34 frames are being saved to U:\krios\20190806\slot11_0153.tif 34 frames are being saved to U:\krios\20190806\slot11_0154.tif Item 115: Stage: -771.35 620.45 70 Label: 113 115 Tick time from program start = 31589.126 Running script 17 at item 114 (-770.27, 618.11) Remaining time to fill dewars is 26160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.29 -0.23 (2nd move error 0.08 -0.04) Finished aligning to item in 1 rounds -3.8 -1.9 unbinned pixels; 1.9 16.5 nm along two shift axes; 8.1 -14.4 nm on specimen axes Total_Shift = 8.485282 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 33.32, -21.91 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 1.2, 8.9 unbinned pixels, 0.16 nm/sec Measured defocus = -1.23 microns changed by -0.87 to target 34 frames are being saved to U:\krios\20190806\slot11_0155.tif 34 frames are being saved to U:\krios\20190806\slot11_0156.tif 34 frames are being saved to U:\krios\20190806\slot11_0157.tif Item 116: Stage: -770.27 618.11 70 Label: 114 116 Tick time from program start = 31707.999 Running script 17 at item 115 (-769.23, 615.77) Remaining time to fill dewars is 26100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.27 -0.22 (2nd move error 0.06 -0.02) Finished aligning to item in 1 rounds -3.6 0.8 unbinned pixels; 7.5 11.6 nm along two shift axes; -2.3 -14.0 nm on specimen axes Total_Shift = 7.286282 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 40.30, -25.93 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -0.2, 9.7 unbinned pixels, 0.17 nm/sec Measured defocus = -1.48 microns changed by -0.72 to target 34 frames are being saved to U:\krios\20190806\slot11_0158.tif 34 frames are being saved to U:\krios\20190806\slot11_0159.tif 34 frames are being saved to U:\krios\20190806\slot11_0160.tif Item 117: Stage: -769.23 615.77 70 Label: 115 117 Tick time from program start = 31824.329 Running script 17 at item 116 (-768.15, 613.48) Remaining time to fill dewars is 25980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.28 -0.23 (2nd move error 0.07 -0.04) Finished aligning to item in 1 rounds -3.9 -0.1 unbinned pixels; 4.7 14.3 nm along two shift axes; 1.3 -15.2 nm on specimen axes Total_Shift = 7.845172 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 42.19, -26.02 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -5.7, 9.1 unbinned pixels, 0.19 nm/sec Measured defocus = -1.53 microns changed by -0.77 to target 34 frames are being saved to U:\krios\20190806\slot11_0161.tif 34 frames are being saved to U:\krios\20190806\slot11_0162.tif 34 frames are being saved to U:\krios\20190806\slot11_0163.tif Item 118: Stage: -768.15 613.48 70 Label: 116 118 Tick time from program start = 31935.713 Running script 17 at item 117 (-766.94, 611.18) Remaining time to fill dewars is 25980 Dewars are NOT busy filling Trimming A by 20 100 50 20, reference by 20 20 20 20 Trimming A by 20 100 50 20, reference by 20 20 20 20 Trimming A by 20 100 50 20, reference by 20 60 20 20 Trimming A by 20 100 50 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.26 -0.28 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to target position: 0.22 -0.19 (2nd move error -0.05 0.07) Finished aligning to item in 2 rounds 1.0 10.2 unbinned pixels; 36.3 18.9 nm along two shift axes; -40.0 1.7 nm on specimen axes Total_Shift = 20.529475 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 41.64, -25.17 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -13.0, 6.1 unbinned pixels, 0.25 nm/sec Measured defocus = -1.50 microns changed by -0.90 to target 34 frames are being saved to U:\krios\20190806\slot11_0164.tif 34 frames are being saved to U:\krios\20190806\slot11_0165.tif 34 frames are being saved to U:\krios\20190806\slot11_0166.tif Item 119: Stage: -766.94 611.18 70 Label: 117 119 Tick time from program start = 32053.416 Running script 17 at item 118 (-769.19, 610.08) Remaining time to fill dewars is 25740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to target position: 0.20 -0.26 (2nd move error -0.06 -0.00) Finished aligning to item in 1 rounds -1.5 11.1 unbinned pixels; 42.7 11.3 nm along two shift axes; -42.9 -8.1 nm on specimen axes Total_Shift = 22.392226 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 76.99, -48.86 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -4.2, 3.6 unbinned pixels, 0.10 nm/sec Measured defocus = -2.82 microns changed by 0.32 to target 34 frames are being saved to U:\krios\20190806\slot11_0167.tif 34 frames are being saved to U:\krios\20190806\slot11_0168.tif 34 frames are being saved to U:\krios\20190806\slot11_0169.tif Item 120: Stage: -769.19 610.08 70 Label: 118 120 Tick time from program start = 32170.963 Running script 17 at item 119 (-770.39, 612.46) Remaining time to fill dewars is 25680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.40 -0.30 (2nd move error 0.19 -0.10) Finished aligning to item in 1 rounds -11.7 -5.3 unbinned pixels; 4.2 50.0 nm along two shift axes; 23.0 -44.3 nm on specimen axes Total_Shift = 25.650375 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 14.75, -6.15 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -4.8, 7.2 unbinned pixels, 0.15 nm/sec Measured defocus = -0.49 microns changed by -0.81 to target 34 frames are being saved to U:\krios\20190806\slot11_0170.tif 34 frames are being saved to U:\krios\20190806\slot11_0171.tif 34 frames are being saved to U:\krios\20190806\slot11_0172.tif Item 121: Stage: -770.39 612.46 70 Label: 119 121 Tick time from program start = 32287.293 Running script 17 at item 120 (-771.47, 614.69) Remaining time to fill dewars is 25560 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.28 -0.25 Disparity in stage position after aligning to target position: 0.30 -0.28 (2nd move error 0.02 -0.06) Finished aligning to item in 2 rounds -7.0 -2.2 unbinned pixels; 1.1 28.6 nm along two shift axes; 10.0 -26.9 nm on specimen axes Total_Shift = 14.725393 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 19.23, -11.32 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -1.8, 4.4 unbinned pixels, 0.09 nm/sec Measured defocus = -0.69 microns changed by -0.71 to target 34 frames are being saved to U:\krios\20190806\slot11_0173.tif 34 frames are being saved to U:\krios\20190806\slot11_0174.tif 34 frames are being saved to U:\krios\20190806\slot11_0175.tif Item 122: Stage: -771.47 614.69 70 Label: 120 122 Tick time from program start = 32404.808 Running script 17 at item 121 (-772.55, 617.04) Remaining time to fill dewars is 25440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.29 (2nd move error -0.05 -0.06) Finished aligning to item in 1 rounds -16.8 28.4 unbinned pixels; 126.5 17.7 nm along two shift axes; -107.0 -71.4 nm on specimen axes Total_Shift = 66.056712 -8.8 20.0 unbinned pixels; 84.9 1.4 nm along two shift axes; -75.9 -38.3 nm on specimen axes Total_Shift = 43.647782 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 22.16, -13.43 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -1.6, 1.2 unbinned pixels, 0.04 nm/sec Measured defocus = -0.80 microns changed by -0.70 to target 34 frames are being saved to U:\krios\20190806\slot11_0176.tif 34 frames are being saved to U:\krios\20190806\slot11_0177.tif 34 frames are being saved to U:\krios\20190806\slot11_0178.tif Item 123: Stage: -772.55 617.04 70 Label: 121 123 Tick time from program start = 32525.116 Running script 17 at item 122 (-773.60, 619.38) Remaining time to fill dewars is 25380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.29 (2nd move error -0.10 -0.06) Finished aligning to item in 1 rounds -7.6 18.2 unbinned pixels; 76.9 0.1 nm along two shift axes; -69.2 -33.6 nm on specimen axes Total_Shift = 39.495255 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 21.44, -12.54 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -1.1, -0.4 unbinned pixels, 0.02 nm/sec Measured defocus = -0.77 microns changed by -0.83 to target 34 frames are being saved to U:\krios\20190806\slot11_0179.tif 34 frames are being saved to U:\krios\20190806\slot11_0180.tif 34 frames are being saved to U:\krios\20190806\slot11_0181.tif Item 124: Stage: -773.60 619.38 70 Label: 122 124 Tick time from program start = 32636.439 Running script 17 at item 123 (-774.71, 621.73) Remaining time to fill dewars is 25320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.22 -0.29 (2nd move error -0.07 -0.07) Finished aligning to item in 1 rounds -9.8 13.7 unbinned pixels; 63.2 14.5 nm along two shift axes; -51.3 -40.9 nm on specimen axes Total_Shift = 33.678022 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 23.46, -12.13 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -1.1, -0.2 unbinned pixels, 0.02 nm/sec Measured defocus = -0.81 microns changed by -0.89 to target 34 frames are being saved to U:\krios\20190806\slot11_0182.tif 34 frames are being saved to U:\krios\20190806\slot11_0183.tif 34 frames are being saved to U:\krios\20190806\slot11_0184.tif Item 125: Stage: -774.71 621.73 70 Label: 123 125 Tick time from program start = 32748.354 Running script 17 at item 124 (-775.77, 624.10) Remaining time to fill dewars is 25200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.10 -0.31 (2nd move error -0.18 -0.08) Finished aligning to item in 1 rounds -10.4 33.8 unbinned pixels; 138.0 13.4 nm along two shift axes; -129.4 -47.7 nm on specimen axes Total_Shift = 70.809247 -7.4 22.1 unbinned pixels; 90.8 6.6 nm along two shift axes; -84.3 -33.4 nm on specimen axes Total_Shift = 46.542994 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 27.26, -16.01 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -0.8, -0.2 unbinned pixels, 0.02 nm/sec Measured defocus = -0.98 microns changed by -0.82 to target 34 frames are being saved to U:\krios\20190806\slot11_0185.tif 34 frames are being saved to U:\krios\20190806\slot11_0186.tif 34 frames are being saved to U:\krios\20190806\slot11_0187.tif Item 126: Stage: -775.77 624.10 70 Label: 124 126 Tick time from program start = 32867.632 Running script 17 at item 125 (-777.15, 626.34) Remaining time to fill dewars is 25080 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.17 -0.29 Disparity in stage position after aligning to target position: 0.44 -0.15 (2nd move error 0.26 0.12) Finished aligning to item in 2 rounds 1.7 5.5 unbinned pixels; 18.0 14.6 nm along two shift axes; -21.8 5.6 nm on specimen axes Total_Shift = 11.571192 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 29.45, -17.44 unbinned pixels, unfiltered CCC is -0.0009 The average drift between shots was -5.8, 4.5 unbinned pixels, 0.13 nm/sec Measured defocus = -1.06 microns changed by -0.84 to target 34 frames are being saved to U:\krios\20190806\slot11_0188.tif 34 frames are being saved to U:\krios\20190806\slot11_0189.tif 34 frames are being saved to U:\krios\20190806\slot11_0190.tif Item 127: Stage: -777.15 626.34 70 Label: 125 127 Tick time from program start = 32988.923 Running script 17 at item 126 (-778.19, 628.58) Remaining time to fill dewars is 25020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.40 -0.21 (2nd move error 0.22 0.06) Finished aligning to item in 1 rounds 0.1 -2.7 unbinned pixels; 9.9 3.8 nm along two shift axes; 10.4 0.8 nm on specimen axes Total_Shift = 5.357264 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 33.18, -19.73 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -6.4, 6.4 unbinned pixels, 0.16 nm/sec Measured defocus = -1.19 microns changed by -0.81 to target 34 frames are being saved to U:\krios\20190806\slot11_0191.tif 34 frames are being saved to U:\krios\20190806\slot11_0192.tif 34 frames are being saved to U:\krios\20190806\slot11_0193.tif Item 128: Stage: -778.19 628.58 70 Label: 126 128 Tick time from program start = 33100.479 Running script 17 at item 127 (-779.23, 630.85) Remaining time to fill dewars is 24900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.39 -0.21 (2nd move error 0.21 0.06) Finished aligning to item in 1 rounds 0.9 0.3 unbinned pixels; 0.1 3.7 nm along two shift axes; -1.3 3.5 nm on specimen axes Total_Shift = 1.904926 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 37.55, -22.55 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -6.7, 6.3 unbinned pixels, 0.16 nm/sec Measured defocus = -1.35 microns changed by -0.75 to target 34 frames are being saved to U:\krios\20190806\slot11_0194.tif 34 frames are being saved to U:\krios\20190806\slot11_0195.tif 34 frames are being saved to U:\krios\20190806\slot11_0196.tif Item 129: Stage: -779.23 630.85 70 Label: 127 129 Tick time from program start = 33211.677 Running script 17 at item 128 (-780.27, 633.21) Remaining time to fill dewars is 24780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.35 -0.23 (2nd move error 0.17 0.04) Finished aligning to item in 1 rounds -1.9 -3.5 unbinned pixels; 10.5 12.3 nm along two shift axes; 14.1 -6.8 nm on specimen axes Total_Shift = 8.050583 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 41.91, -25.22 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -7.7, 7.3 unbinned pixels, 0.19 nm/sec Measured defocus = -1.51 microns changed by -0.69 to target 34 frames are being saved to U:\krios\20190806\slot11_0197.tif 34 frames are being saved to U:\krios\20190806\slot11_0198.tif 34 frames are being saved to U:\krios\20190806\slot11_0199.tif Item 130: Stage: -780.27 633.21 70 Label: 128 130 Tick time from program start = 33322.905 Running script 17 at item 129 (-781.35, 635.57) Remaining time to fill dewars is 24720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.24 (2nd move error 0.06 0.02) Finished aligning to item in 1 rounds 0.5 -0.6 unbinned pixels; 2.8 1.0 nm along two shift axes; 2.2 2.1 nm on specimen axes Total_Shift = 1.56493 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 45.41, -28.86 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -7.5, 7.0 unbinned pixels, 0.18 nm/sec Measured defocus = -1.66 microns changed by -0.64 to target 34 frames are being saved to U:\krios\20190806\slot11_0200.tif 34 frames are being saved to U:\krios\20190806\slot11_0201.tif 34 frames are being saved to U:\krios\20190806\slot11_0202.tif Item 131: Stage: -781.35 635.57 70 Label: 129 131 Tick time from program start = 33434.087 Running script 17 at item 130 (-782.65, 638.09) Remaining time to fill dewars is 24600 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.15 -0.39 Disparity in stage position after aligning to target position: 0.43 -0.44 (2nd move error 0.28 -0.07) Finished aligning to item in 2 rounds -6.3 -6.2 unbinned pixels; 14.5 32.2 nm along two shift axes; 25.5 -23.4 nm on specimen axes Total_Shift = 17.745037 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 48.53, -31.58 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -0.9, 9.8 unbinned pixels, 0.17 nm/sec Measured defocus = -1.79 microns changed by -0.61 to target 34 frames are being saved to U:\krios\20190806\slot11_0203.tif 34 frames are being saved to U:\krios\20190806\slot11_0204.tif 34 frames are being saved to U:\krios\20190806\slot11_0205.tif Item 132: Stage: -782.65 638.09 70 Label: 130 132 Tick time from program start = 33555.051 Running script 17 at item 131 (-783.71, 640.35) Remaining time to fill dewars is 24480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.31 -0.43 (2nd move error 0.16 -0.06) Finished aligning to item in 1 rounds -8.0 -6.7 unbinned pixels; 14.2 39.0 nm along two shift axes; 27.8 -29.8 nm on specimen axes Total_Shift = 20.938416 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 53.16, -34.73 unbinned pixels, unfiltered CCC is 0.7315 The average drift between shots was 0.0, 12.7 unbinned pixels, 0.23 nm/sec Measured defocus = -1.96 microns changed by -0.54 to target 34 frames are being saved to U:\krios\20190806\slot11_0206.tif 34 frames are being saved to U:\krios\20190806\slot11_0207.tif 34 frames are being saved to U:\krios\20190806\slot11_0208.tif Item 133: Stage: -783.71 640.35 70 Label: 131 133 Tick time from program start = 33671.131 Running script 17 at item 132 (-785.81, 645.02) Remaining time to fill dewars is 24360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.30 -0.39 (2nd move error 0.14 -0.02) Finished aligning to item in 1 rounds -2.7 -3.0 unbinned pixels; 7.6 14.2 nm along two shift axes; 12.3 -9.8 nm on specimen axes Total_Shift = 8.074176 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 16.42, -10.35 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -1.2, 8.2 unbinned pixels, 0.15 nm/sec Measured defocus = -0.60 microns changed by -0.70 to target 34 frames are being saved to U:\krios\20190806\slot11_0209.tif 34 frames are being saved to U:\krios\20190806\slot11_0210.tif 34 frames are being saved to U:\krios\20190806\slot11_0211.tif Item 134: Stage: -785.81 645.02 70 Label: 132 134 Tick time from program start = 33782.952 Running script 17 at item 133 (-786.85, 647.38) Remaining time to fill dewars is 24300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.12 -0.42 (2nd move error -0.04 -0.05) Finished aligning to item in 1 rounds -9.8 9.3 unbinned pixels; 47.0 21.3 nm along two shift axes; -34.1 -40.1 nm on specimen axes Total_Shift = 27.043559 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 14.43, -8.55 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -2.4, 2.5 unbinned pixels, 0.06 nm/sec Measured defocus = -0.52 microns changed by -0.78 to target 34 frames are being saved to U:\krios\20190806\slot11_0212.tif 34 frames are being saved to U:\krios\20190806\slot11_0213.tif 34 frames are being saved to U:\krios\20190806\slot11_0214.tif Item 135: Stage: -786.85 647.38 70 Label: 133 135 Tick time from program start = 33894.150 Running script 17 at item 134 (-790.19, 648.64) Remaining time to fill dewars is 24180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 55 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.10 -0.45 (2nd move error -0.06 -0.08) Finished aligning to item in 1 rounds Trimming A by 55 20 20 20, reference by 55 20 20 20 -15.6 20.4 unbinned pixels; 95.4 25.5 nm along two shift axes; -76.1 -65.0 nm on specimen axes Total_Shift = 51.378736 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 8.61, -7.94 unbinned pixels, unfiltered CCC is 0.0065 The average drift between shots was -3.6, 3.5 unbinned pixels, 0.09 nm/sec Measured defocus = -0.36 microns changed by -1.04 to target 34 frames are being saved to U:\krios\20190806\slot11_0215.tif 34 frames are being saved to U:\krios\20190806\slot11_0216.tif 34 frames are being saved to U:\krios\20190806\slot11_0217.tif Item 136: Stage: -790.19 648.64 70 Label: 134 136 Tick time from program start = 34006.751 Running script 17 at item 135 (-789.18, 646.32) Remaining time to fill dewars is 24060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.22 -0.45 (2nd move error 0.07 -0.08) Finished aligning to item in 1 rounds -8.2 -2.3 unbinned pixels; 2.1 32.9 nm along two shift axes; 10.8 -31.3 nm on specimen axes Total_Shift = 17.004535 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 19.29, -13.63 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -0.6, 11.4 unbinned pixels, 0.20 nm/sec Measured defocus = -0.73 microns changed by -0.77 to target 34 frames are being saved to U:\krios\20190806\slot11_0218.tif 34 frames are being saved to U:\krios\20190806\slot11_0219.tif 34 frames are being saved to U:\krios\20190806\slot11_0220.tif Item 137: Stage: -789.18 646.32 70 Label: 135 137 Tick time from program start = 34118.074 Running script 17 at item 136 (-788.08, 643.92) Remaining time to fill dewars is 23940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.29 -0.41 (2nd move error 0.14 -0.04) Finished aligning to item in 1 rounds -3.3 5.2 unbinned pixels; 23.3 4.0 nm along two shift axes; -19.4 -13.9 nm on specimen axes Total_Shift = 12.255766 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 22.50, -16.10 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -1.0, 8.0 unbinned pixels, 0.14 nm/sec Measured defocus = -0.85 microns changed by -0.75 to target 34 frames are being saved to U:\krios\20190806\slot11_0221.tif 34 frames are being saved to U:\krios\20190806\slot11_0222.tif 34 frames are being saved to U:\krios\20190806\slot11_0223.tif Item 138: Stage: -788.08 643.92 70 Label: 136 138 Tick time from program start = 34231.861 Running script 17 at item 137 (-787.03, 641.66) Remaining time to fill dewars is 23880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.22 -0.41 (2nd move error 0.07 -0.04) Finished aligning to item in 1 rounds -2.8 -0.9 unbinned pixels; 0.2 11.6 nm along two shift axes; 4.3 -10.8 nm on specimen axes Total_Shift = 5.938095 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 30.71, -20.87 unbinned pixels, unfiltered CCC is -0.0014 The average drift between shots was -1.7, 8.2 unbinned pixels, 0.15 nm/sec Measured defocus = -1.15 microns changed by -0.55 to target 34 frames are being saved to U:\krios\20190806\slot11_0224.tif 34 frames are being saved to U:\krios\20190806\slot11_0225.tif 34 frames are being saved to U:\krios\20190806\slot11_0226.tif Item 139: Stage: -787.03 641.66 70 Label: 137 139 Tick time from program start = 34343.230 Running script 17 at item 138 (-785.95, 639.36) Remaining time to fill dewars is 23760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.42 -0.42 (2nd move error 0.27 -0.07) Finished aligning to item in 1 rounds -5.3 -4.4 unbinned pixels; 9.1 25.7 nm along two shift axes; 18.1 -19.7 nm on specimen axes Total_Shift = 13.738936 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 36.75, -24.64 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -0.5, 12.2 unbinned pixels, 0.21 nm/sec Measured defocus = -1.37 microns changed by -0.43 to target 34 frames are being saved to U:\krios\20190806\slot11_0227.tif 34 frames are being saved to U:\krios\20190806\slot11_0228.tif 34 frames are being saved to U:\krios\20190806\slot11_0229.tif Item 140: Stage: -785.95 639.36 70 Label: 138 140 Tick time from program start = 34454.537 Running script 17 at item 139 (-784.68, 636.84) Remaining time to fill dewars is 23640 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.26 -0.25 Disparity in stage position after aligning to target position: 0.12 -0.25 (2nd move error -0.14 -0.02) Finished aligning to item in 2 rounds -4.3 25.9 unbinned pixels; 101.0 23.4 nm along two shift axes; -100.0 -22.4 nm on specimen axes Total_Shift = 52.583589 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 38.38, -22.10 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -4.0, 0.5 unbinned pixels, 0.07 nm/sec Measured defocus = -1.37 microns changed by -0.53 to target 34 frames are being saved to U:\krios\20190806\slot11_0230.tif 34 frames are being saved to U:\krios\20190806\slot11_0231.tif 34 frames are being saved to U:\krios\20190806\slot11_0232.tif Item 141: Stage: -784.68 636.84 70 Label: 139 141 Tick time from program start = 34578.947 Running script 17 at item 140 (-783.64, 634.51) Remaining time to fill dewars is 23460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.22 -0.25 (2nd move error -0.05 -0.03) Finished aligning to item in 1 rounds -4.2 11.7 unbinned pixels; 48.6 2.5 nm along two shift axes; -44.8 -18.8 nm on specimen axes Total_Shift = 24.936125 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 41.44, -23.37 unbinned pixels, unfiltered CCC is -0.0011 The average drift between shots was -3.8, 1.0 unbinned pixels, 0.07 nm/sec Measured defocus = -1.47 microns changed by -0.53 to target 34 frames are being saved to U:\krios\20190806\slot11_0233.tif 34 frames are being saved to U:\krios\20190806\slot11_0234.tif 34 frames are being saved to U:\krios\20190806\slot11_0235.tif Item 142: Stage: -783.64 634.51 70 Label: 140 142 Tick time from program start = 34689.989 Running script 17 at item 141 (-782.54, 632.15) Remaining time to fill dewars is 23340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.32 -0.22 (2nd move error 0.06 0.01) Finished aligning to item in 1 rounds 1.4 -1.7 unbinned pixels; 8.2 2.6 nm along two shift axes; 6.3 6.0 nm on specimen axes Total_Shift = 4.474487 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 42.70, -25.12 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -5.3, 5.6 unbinned pixels, 0.14 nm/sec Measured defocus = -1.53 microns changed by -0.57 to target 34 frames are being saved to U:\krios\20190806\slot11_0236.tif 34 frames are being saved to U:\krios\20190806\slot11_0237.tif 34 frames are being saved to U:\krios\20190806\slot11_0238.tif Item 143: Stage: -782.54 632.15 70 Label: 141 143 Tick time from program start = 34804.026 Running script 17 at item 142 (-781.47, 629.76) Remaining time to fill dewars is 23220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.29 -0.22 (2nd move error 0.03 0.00) Finished aligning to item in 1 rounds -0.0 -1.4 unbinned pixels; 5.1 2.2 nm along two shift axes; 5.4 0.1 nm on specimen axes Total_Shift = 2.78676 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 41.73, -26.63 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -3.8, 7.3 unbinned pixels, 0.15 nm/sec Measured defocus = -1.53 microns changed by -0.67 to target 34 frames are being saved to U:\krios\20190806\slot11_0239.tif 34 frames are being saved to U:\krios\20190806\slot11_0240.tif 34 frames are being saved to U:\krios\20190806\slot11_0241.tif Item 144: Stage: -781.47 629.76 70 Label: 142 144 Tick time from program start = 34921.775 Running script 17 at item 143 (-780.40, 627.51) Remaining time to fill dewars is 23160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.40 -0.18 (2nd move error 0.14 0.04) Finished aligning to item in 1 rounds 0.9 -0.4 unbinned pixels; 2.6 2.8 nm along two shift axes; 1.2 3.7 nm on specimen axes Total_Shift = 1.995138 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 45.52, -28.05 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -4.4, 6.3 unbinned pixels, 0.14 nm/sec Measured defocus = -1.65 microns changed by -0.65 to target 34 frames are being saved to U:\krios\20190806\slot11_0242.tif 34 frames are being saved to U:\krios\20190806\slot11_0243.tif 34 frames are being saved to U:\krios\20190806\slot11_0244.tif Item 145: Stage: -780.40 627.51 70 Label: 143 145 Tick time from program start = 35032.926 Running script 17 at item 144 (-779.36, 625.24) Remaining time to fill dewars is 23040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.44 -0.21 (2nd move error 0.17 0.02) Finished aligning to item in 1 rounds 2.9 1.1 unbinned pixels; 0.4 12.1 nm along two shift axes; -5.0 11.0 nm on specimen axes Total_Shift = 6.178867 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 48.61, -28.84 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -3.2, 7.6 unbinned pixels, 0.15 nm/sec Measured defocus = -1.75 microns changed by -0.65 to target 34 frames are being saved to U:\krios\20190806\slot11_0245.tif 34 frames are being saved to U:\krios\20190806\slot11_0246.tif 34 frames are being saved to U:\krios\20190806\slot11_0247.tif Item 146: Stage: -779.36 625.24 70 Label: 144 146 Tick time from program start = 35144.186 Running script 17 at item 145 (-777.92, 622.85) Remaining time to fill dewars is 22980 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.19 -0.30 Disparity in stage position after aligning to target position: 0.12 -0.19 (2nd move error -0.08 0.09) Finished aligning to item in 2 rounds -6.8 21.5 unbinned pixels; 87.8 8.0 nm along two shift axes; -82.2 -30.9 nm on specimen axes Total_Shift = 45.050739 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 49.47, -30.26 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -2.0, 0.4 unbinned pixels, 0.04 nm/sec Measured defocus = -1.79 microns changed by -0.71 to target 34 frames are being saved to U:\krios\20190806\slot11_0248.tif 34 frames are being saved to U:\krios\20190806\slot11_0249.tif 34 frames are being saved to U:\krios\20190806\slot11_0250.tif Item 147: Stage: -777.92 622.85 70 Label: 145 147 Tick time from program start = 35263.199 Running script 17 at item 146 (-776.88, 620.57) Remaining time to fill dewars is 22860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.22 (2nd move error -0.04 0.06) Finished aligning to item in 1 rounds -0.6 9.1 unbinned pixels; 34.4 11.7 nm along two shift axes; -35.5 -4.2 nm on specimen axes Total_Shift = 18.331687 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 20.16, -11.39 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -9.4, 0.9 unbinned pixels, 0.17 nm/sec Measured defocus = -0.72 microns changed by -0.58 to target 34 frames are being saved to U:\krios\20190806\slot11_0251.tif 34 frames are being saved to U:\krios\20190806\slot11_0252.tif 34 frames are being saved to U:\krios\20190806\slot11_0253.tif Item 148: Stage: -776.88 620.57 70 Label: 146 148 Tick time from program start = 35374.241 Running script 17 at item 147 (-775.85, 618.33) Remaining time to fill dewars is 22800 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.22 Disparity in stage position after aligning to target position: 0.07 -0.33 (2nd move error -0.05 -0.13) Finished aligning to item in 2 rounds -16.8 10.9 unbinned pixels; 61.9 44.0 nm along two shift axes; -38.8 -67.6 nm on specimen axes Total_Shift = 39.99433 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 22.39, -11.72 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -4.2, 1.4 unbinned pixels, 0.08 nm/sec Measured defocus = -0.78 microns changed by -0.62 to target 34 frames are being saved to U:\krios\20190806\slot11_0254.tif 34 frames are being saved to U:\krios\20190806\slot11_0255.tif 34 frames are being saved to U:\krios\20190806\slot11_0256.tif Item 149: Stage: -775.85 618.33 70 Label: 147 149 Tick time from program start = 35495.063 Running script 17 at item 148 (-774.75, 616.02) Remaining time to fill dewars is 22680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.33 (2nd move error 0.07 -0.12) Finished aligning to item in 1 rounds -12.9 -2.0 unbinned pixels; 9.6 49.5 nm along two shift axes; 10.4 -49.8 nm on specimen axes Total_Shift = 26.127633 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 23.88, -13.57 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -2.5, 6.8 unbinned pixels, 0.13 nm/sec Measured defocus = -0.85 microns changed by -0.65 to target 34 frames are being saved to U:\krios\20190806\slot11_0257.tif 34 frames are being saved to U:\krios\20190806\slot11_0258.tif 34 frames are being saved to U:\krios\20190806\slot11_0259.tif Item 150: Stage: -774.75 616.02 70 Label: 148 150 Tick time from program start = 35606.448 Running script 17 at item 149 (-773.67, 613.67) Remaining time to fill dewars is 22560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.29 (2nd move error 0.07 -0.09) Finished aligning to item in 1 rounds -9.0 -0.8 unbinned pixels; 8.7 33.8 nm along two shift axes; 5.2 -34.9 nm on specimen axes Total_Shift = 18.135918 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 26.22, -13.52 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -4.9, 7.9 unbinned pixels, 0.17 nm/sec Measured defocus = -0.91 microns changed by -0.69 to target 34 frames are being saved to U:\krios\20190806\slot11_0260.tif 34 frames are being saved to U:\krios\20190806\slot11_0261.tif 34 frames are being saved to U:\krios\20190806\slot11_0262.tif Item 151: Stage: -773.67 613.67 70 Label: 149 151 Tick time from program start = 35717.786 Running script 17 at item 150 (-772.59, 611.42) Remaining time to fill dewars is 22500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.29 (2nd move error 0.11 -0.09) Finished aligning to item in 1 rounds -9.3 -3.9 unbinned pixels; 2.0 39.4 nm along two shift axes; 17.0 -35.5 nm on specimen axes Total_Shift = 20.191498 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 24.97, -13.23 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -4.7, 10.5 unbinned pixels, 0.21 nm/sec Measured defocus = -0.87 microns changed by -0.83 to target 34 frames are being saved to U:\krios\20190806\slot11_0263.tif 34 frames are being saved to U:\krios\20190806\slot11_0264.tif 34 frames are being saved to U:\krios\20190806\slot11_0265.tif Item 152: Stage: -772.59 611.42 70 Label: 150 152 Tick time from program start = 35829.202 Running script 17 at item 151 (-771.43, 609.02) Remaining time to fill dewars is 22380 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 20 100 60 20, reference by 20 20 20 20 Trimming A by 20 100 60 20, reference by 20 20 20 20 Trimming A by 20 100 60 20, reference by 20 60 20 20 Trimming A by 20 100 60 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.23 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to target position: 0.13 -0.28 (2nd move error -0.02 -0.07) Finished aligning to item in 2 rounds -13.0 18.8 unbinned pixels; 86.1 18.5 nm along two shift axes; -70.4 -54.5 nm on specimen axes Total_Shift = 45.72266 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 23.86, -12.65 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -8.3, 1.8 unbinned pixels, 0.15 nm/sec Measured defocus = -0.83 microns changed by -0.97 to target 34 frames are being saved to U:\krios\20190806\slot11_0266.tif 34 frames are being saved to U:\krios\20190806\slot11_0267.tif 34 frames are being saved to U:\krios\20190806\slot11_0268.tif Item 153: Stage: -771.43 609.02 70 Label: 151 153 Tick time from program start = 35965.469 Running script 17 at item 152 (-772.59, 605.55) Remaining time to fill dewars is 22140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 45 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to target position: 0.17 -0.33 (2nd move error 0.02 -0.12) Finished aligning to item in 1 rounds Trimming A by 20 45 20 20, reference by 20 45 20 20 -16.0 -1.5 unbinned pixels; 15.6 59.9 nm along two shift axes; 9.1 -62.1 nm on specimen axes Total_Shift = 32.203071 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 22.92, -9.33 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -6.3, 2.8 unbinned pixels, 0.12 nm/sec Measured defocus = -0.76 microns changed by -1.14 to target 34 frames are being saved to U:\krios\20190806\slot11_0269.tif 34 frames are being saved to U:\krios\20190806\slot11_0270.tif 34 frames are being saved to U:\krios\20190806\slot11_0271.tif Item 154: Stage: -772.59 605.55 70 Label: 152 154 Tick time from program start = 36077.103 Running script 17 at item 153 (-773.71, 607.92) Remaining time to fill dewars is 22080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to target position: 0.17 -0.36 (2nd move error 0.01 -0.15) Finished aligning to item in 1 rounds -19.6 -0.5 unbinned pixels; 23.9 71.2 nm along two shift axes; 5.9 -76.1 nm on specimen axes Total_Shift = 39.208811 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 30.62, -16.76 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -2.8, 4.9 unbinned pixels, 0.10 nm/sec Measured defocus = -1.08 microns changed by -0.92 to target 34 frames are being saved to U:\krios\20190806\slot11_0272.tif 34 frames are being saved to U:\krios\20190806\slot11_0273.tif 34 frames are being saved to U:\krios\20190806\slot11_0274.tif Item 155: Stage: -773.71 607.92 70 Label: 153 155 Tick time from program start = 36188.706 Running script 17 at item 154 (-774.79, 610.30) Remaining time to fill dewars is 21960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to target position: 0.16 -0.32 (2nd move error 0.01 -0.11) Finished aligning to item in 1 rounds -14.9 -0.6 unbinned pixels; 17.1 54.4 nm along two shift axes; 5.6 -57.7 nm on specimen axes Total_Shift = 29.747245 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 35.90, -21.51 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -2.6, 6.1 unbinned pixels, 0.12 nm/sec Measured defocus = -1.29 microns changed by -0.81 to target 34 frames are being saved to U:\krios\20190806\slot11_0275.tif 34 frames are being saved to U:\krios\20190806\slot11_0276.tif 34 frames are being saved to U:\krios\20190806\slot11_0277.tif Item 156: Stage: -774.79 610.30 70 Label: 154 156 Tick time from program start = 36300.481 Running script 17 at item 155 (-775.91, 612.55) Remaining time to fill dewars is 21840 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.31 Disparity in stage position after aligning to target position: 0.26 -0.18 (2nd move error 0.17 0.11) Finished aligning to item in 2 rounds 1.7 3.2 unbinned pixels; 9.5 10.7 nm along two shift axes; -12.7 5.8 nm on specimen axes Total_Shift = 7.143038 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 40.54, -26.02 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -9.0, 10.2 unbinned pixels, 0.24 nm/sec Measured defocus = -1.49 microns changed by -0.71 to target 34 frames are being saved to U:\krios\20190806\slot11_0278.tif 34 frames are being saved to U:\krios\20190806\slot11_0279.tif 34 frames are being saved to U:\krios\20190806\slot11_0280.tif Item 157: Stage: -775.91 612.55 70 Label: 155 157 Tick time from program start = 36422.411 Running script 17 at item 156 (-777.04, 614.79) Remaining time to fill dewars is 21720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.27 -0.21 (2nd move error 0.17 0.08) Finished aligning to item in 1 rounds 1.2 1.2 unbinned pixels; 2.9 6.3 nm along two shift axes; -5.0 4.6 nm on specimen axes Total_Shift = 3.491259 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 54.86, -30.52 unbinned pixels, unfiltered CCC is -0.0009 The average drift between shots was -20.0, 8.1 unbinned pixels, 0.38 nm/sec Measured defocus = -1.94 microns changed by -0.36 to target 34 frames are being saved to U:\krios\20190806\slot11_0281.tif 34 frames are being saved to U:\krios\20190806\slot11_0282.tif 34 frames are being saved to U:\krios\20190806\slot11_0283.tif Item 158: Stage: -777.04 614.79 70 Label: 156 158 Tick time from program start = 36540.161 Running script 17 at item 157 (-778.09, 617.13) Remaining time to fill dewars is 21660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.25 (2nd move error 0.14 0.04) Finished aligning to item in 1 rounds -1.2 -2.8 unbinned pixels; 8.9 8.5 nm along two shift axes; 11.3 -4.0 nm on specimen axes Total_Shift = 6.148159 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 51.96, -31.33 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -8.0, 9.4 unbinned pixels, 0.22 nm/sec Measured defocus = -1.88 microns changed by -0.52 to target 34 frames are being saved to U:\krios\20190806\slot11_0284.tif 34 frames are being saved to U:\krios\20190806\slot11_0285.tif 34 frames are being saved to U:\krios\20190806\slot11_0286.tif Item 159: Stage: -778.09 617.13 70 Label: 157 159 Tick time from program start = 36652.045 Running script 17 at item 158 (-780.23, 621.77) Remaining time to fill dewars is 21540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.16 -0.28 (2nd move error 0.07 0.01) Finished aligning to item in 1 rounds 1.4 -4.9 unbinned pixels; 19.7 2.2 nm along two shift axes; 18.6 6.6 nm on specimen axes Total_Shift = 10.121136 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 54.76, -35.32 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.2, 9.1 unbinned pixels, 0.18 nm/sec Measured defocus = -2.01 microns changed by -0.49 to target 34 frames are being saved to U:\krios\20190806\slot11_0287.tif 34 frames are being saved to U:\krios\20190806\slot11_0288.tif 34 frames are being saved to U:\krios\20190806\slot11_0289.tif Item 160: Stage: -780.23 621.77 70 Label: 158 160 Tick time from program start = 36763.695 Running script 17 at item 159 (-781.26, 624.14) Remaining time to fill dewars is 21420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.02 -0.27 (2nd move error -0.07 0.02) Finished aligning to item in 1 rounds 1.6 12.7 unbinned pixels; 44.6 24.7 nm along two shift axes; -49.6 3.4 nm on specimen axes Total_Shift = 25.542223 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 20.99, -10.00 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -7.7, 0.4 unbinned pixels, 0.14 nm/sec Measured defocus = -0.71 microns changed by -0.59 to target 34 frames are being saved to U:\krios\20190806\slot11_0290.tif 34 frames are being saved to U:\krios\20190806\slot11_0291.tif 34 frames are being saved to U:\krios\20190806\slot11_0292.tif Item 161: Stage: -781.26 624.14 70 Label: 159 161 Tick time from program start = 36875.641 Running script 17 at item 160 (-782.67, 626.42) Remaining time to fill dewars is 21240 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.25 -0.30 Disparity in stage position after aligning to target position: 0.39 -0.26 (2nd move error 0.13 0.02) Finished aligning to item in 2 rounds 2.4 -3.1 unbinned pixels; 14.4 3.9 nm along two shift axes; 11.4 9.9 nm on specimen axes Total_Shift = 7.763975 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 19.39, -8.59 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -6.2, 5.4 unbinned pixels, 0.14 nm/sec Measured defocus = -0.65 microns changed by -0.65 to target 34 frames are being saved to U:\krios\20190806\slot11_0293.tif 34 frames are being saved to U:\krios\20190806\slot11_0294.tif 34 frames are being saved to U:\krios\20190806\slot11_0295.tif Item 162: Stage: -782.67 626.42 70 Label: 160 162 Tick time from program start = 36997.447 Running script 17 at item 161 (-783.73, 628.72) Remaining time to fill dewars is 21180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.27 -0.27 (2nd move error 0.02 0.01) Finished aligning to item in 1 rounds 1.5 -2.6 unbinned pixels; 11.6 1.3 nm along two shift axes; 9.9 6.2 nm on specimen axes Total_Shift = 6.006597 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 23.22, -12.65 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -6.8, 4.8 unbinned pixels, 0.15 nm/sec Measured defocus = -0.82 microns changed by -0.58 to target 34 frames are being saved to U:\krios\20190806\slot11_0296.tif 34 frames are being saved to U:\krios\20190806\slot11_0297.tif 34 frames are being saved to U:\krios\20190806\slot11_0298.tif Item 163: Stage: -783.73 628.72 70 Label: 161 163 Tick time from program start = 37108.816 Running script 17 at item 162 (-784.80, 631.04) Remaining time to fill dewars is 21060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.18 -0.28 (2nd move error -0.07 -0.00) Finished aligning to item in 1 rounds -1.4 13.0 unbinned pixels; 49.8 14.6 nm along two shift axes; -50.5 -8.2 nm on specimen axes Total_Shift = 26.247024 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 26.74, -15.26 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -5.5, 1.1 unbinned pixels, 0.10 nm/sec Measured defocus = -0.95 microns changed by -0.55 to target 34 frames are being saved to U:\krios\20190806\slot11_0299.tif 34 frames are being saved to U:\krios\20190806\slot11_0300.tif 34 frames are being saved to U:\krios\20190806\slot11_0301.tif Item 164: Stage: -784.80 631.04 70 Label: 162 164 Tick time from program start = 37221.932 Running script 17 at item 163 (-785.83, 633.43) Remaining time to fill dewars is 20880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.28 -0.32 (2nd move error 0.02 -0.04) Finished aligning to item in 1 rounds -4.9 -2.3 unbinned pixels; 2.1 21.2 nm along two shift axes; 10.0 -18.7 nm on specimen axes Total_Shift = 10.875496 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 29.85, -19.43 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -2.0, 6.8 unbinned pixels, 0.13 nm/sec Measured defocus = -1.10 microns changed by -0.50 to target 34 frames are being saved to U:\krios\20190806\slot11_0302.tif 34 frames are being saved to U:\krios\20190806\slot11_0303.tif 34 frames are being saved to U:\krios\20190806\slot11_0304.tif Item 165: Stage: -785.83 633.43 70 Label: 163 165 Tick time from program start = 37333.410 Running script 17 at item 164 (-786.98, 635.79) Remaining time to fill dewars is 20700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.09 -0.32 (2nd move error -0.18 -0.04) Finished aligning to item in 1 rounds -6.7 33.3 unbinned pixels; 131.3 26.1 nm along two shift axes; -128.3 -33.0 nm on specimen axes Total_Shift = 67.992467 -4.2 23.8 unbinned pixels; 92.9 20.8 nm along two shift axes; -91.6 -21.2 nm on specimen axes Total_Shift = 48.274892 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 33.73, -20.55 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -3.4, 4.0 unbinned pixels, 0.09 nm/sec Measured defocus = -1.22 microns changed by -0.48 to target 34 frames are being saved to U:\krios\20190806\slot11_0305.tif 34 frames are being saved to U:\krios\20190806\slot11_0306.tif 34 frames are being saved to U:\krios\20190806\slot11_0307.tif Item 166: Stage: -786.98 635.79 70 Label: 164 166 Tick time from program start = 37451.051 Running script 17 at item 165 (-788.14, 638.30) Remaining time to fill dewars is 20580 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.12 -0.32 Disparity in stage position after aligning to target position: 0.31 -0.52 (2nd move error 0.19 -0.22) Finished aligning to item in 2 rounds -25.4 2.1 unbinned pixels; 40.8 88.4 nm along two shift axes; -2.7 -99.3 nm on specimen axes Total_Shift = 51.00741 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 32.29, -21.00 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was 3.1, 9.2 unbinned pixels, 0.17 nm/sec Measured defocus = -1.19 microns changed by -0.61 to target 34 frames are being saved to U:\krios\20190806\slot11_0308.tif 34 frames are being saved to U:\krios\20190806\slot11_0309.tif 34 frames are being saved to U:\krios\20190806\slot11_0310.tif Item 167: Stage: -788.14 638.30 70 Label: 165 167 Tick time from program start = 37570.157 Running script 17 at item 166 (-789.18, 640.59) Remaining time to fill dewars is 20520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.24 -0.47 (2nd move error 0.12 -0.17) Finished aligning to item in 1 rounds -21.4 -3.9 unbinned pixels; 13.7 83.0 nm along two shift axes; 19.6 -82.5 nm on specimen axes Total_Shift = 43.548356 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 31.46, -22.07 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was 2.4, 13.4 unbinned pixels, 0.24 nm/sec Measured defocus = -1.19 microns changed by -0.71 to target 34 frames are being saved to U:\krios\20190806\slot11_0311.tif 34 frames are being saved to U:\krios\20190806\slot11_0312.tif 34 frames are being saved to U:\krios\20190806\slot11_0313.tif Item 168: Stage: -789.18 640.59 70 Label: 166 168 Tick time from program start = 37681.807 Running script 17 at item 167 (-790.36, 642.70) Remaining time to fill dewars is 20340 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.04 -0.36 Disparity in stage position after aligning to target position: 0.22 -0.24 (2nd move error 0.18 0.10) Finished aligning to item in 2 rounds -0.3 -6.3 unbinned pixels; 22.9 10.5 nm along two shift axes; 24.7 0.3 nm on specimen axes Total_Shift = 12.678709 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 29.27, -23.42 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -3.2, 17.7 unbinned pixels, 0.32 nm/sec Measured defocus = -1.15 microns changed by -0.85 to target 34 frames are being saved to U:\krios\20190806\slot11_0314.tif 34 frames are being saved to U:\krios\20190806\slot11_0315.tif 34 frames are being saved to U:\krios\20190806\slot11_0316.tif Item 169: Stage: -790.36 642.70 70 Label: 167 169 Tick time from program start = 37803.161 Running script 17 at item 168 (-791.41, 645.07) Remaining time to fill dewars is 20220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.28 -0.27 (2nd move error 0.24 0.06) Finished aligning to item in 1 rounds -2.0 5.0 unbinned pixels; 20.8 0.4 nm along two shift axes; -18.9 -8.7 nm on specimen axes Total_Shift = 10.669859 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 29.06, -21.12 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -5.2, 12.4 unbinned pixels, 0.24 nm/sec Measured defocus = -1.11 microns changed by -0.99 to target 34 frames are being saved to U:\krios\20190806\slot11_0317.tif 34 frames are being saved to U:\krios\20190806\slot11_0318.tif 34 frames are being saved to U:\krios\20190806\slot11_0319.tif Item 170: Stage: -791.41 645.07 70 Label: 168 170 Tick time from program start = 37916.402 Running script 17 at item 169 (-792.46, 647.38) Remaining time to fill dewars is 20100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.31 (2nd move error 0.18 0.02) Finished aligning to item in 1 rounds -0.4 -5.5 unbinned pixels; 19.9 9.7 nm along two shift axes; 21.6 -0.3 nm on specimen axes Total_Shift = 11.105518 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 28.64, -20.56 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -3.6, 15.3 unbinned pixels, 0.28 nm/sec Measured defocus = -1.09 microns changed by -1.11 to target 34 frames are being saved to U:\krios\20190806\slot11_0320.tif 34 frames are being saved to U:\krios\20190806\slot11_0321.tif 34 frames are being saved to U:\krios\20190806\slot11_0322.tif Item 171: Stage: -792.46 647.38 70 Label: 169 171 Tick time from program start = 38028.504 Running script 17 at item 170 (-793.50, 649.74) Remaining time to fill dewars is 19980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 100 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.13 -0.30 (2nd move error 0.08 0.04) Finished aligning to item in 1 rounds Trimming A by 100 20 20 20, reference by 100 20 20 20 -0.1 -1.9 unbinned pixels; 6.9 3.3 nm along two shift axes; 7.5 0.0 nm on specimen axes Total_Shift = 3.855081 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 62.32, -43.53 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -2.4, 15.2 unbinned pixels, 0.27 nm/sec Measured defocus = -2.35 microns changed by 0.05 to target 34 frames are being saved to U:\krios\20190806\slot11_0323.tif 34 frames are being saved to U:\krios\20190806\slot11_0324.tif 34 frames are being saved to U:\krios\20190806\slot11_0325.tif Item 172: Stage: -793.50 649.74 70 Label: 170 172 Tick time from program start = 38140.481 Running script 17 at item 171 (-795.78, 648.67) Remaining time to fill dewars is 19860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.13 -0.34 (2nd move error 0.07 -0.01) Finished aligning to item in 1 rounds Trimming A by 70 20 20 20, reference by 70 20 20 20 -2.5 -1.0 unbinned pixels; 0.3 10.4 nm along two shift axes; 4.3 -9.5 nm on specimen axes Total_Shift = 5.335705 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 27.80, -16.67 unbinned pixels, unfiltered CCC is 0.0068 The average drift between shots was -4.6, 9.3 unbinned pixels, 0.18 nm/sec Measured defocus = -1.00 microns changed by -1.40 to target 34 frames are being saved to U:\krios\20190806\slot11_0326.tif 34 frames are being saved to U:\krios\20190806\slot11_0327.tif 34 frames are being saved to U:\krios\20190806\slot11_0328.tif Item 173: Stage: -795.78 648.67 70 Label: 171 173 Tick time from program start = 38255.517 Running script 17 at item 172 (-794.70, 646.27) Remaining time to fill dewars is 19620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.26 -0.34 (2nd move error 0.21 -0.01) Finished aligning to item in 1 rounds -0.9 0.9 unbinned pixels; 4.6 1.9 nm along two shift axes; -3.4 -3.8 nm on specimen axes Total_Shift = 2.606416 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 68.26, -44.12 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -1.3, 11.3 unbinned pixels, 0.20 nm/sec Measured defocus = -2.51 microns changed by 0.01 to target 34 frames are being saved to U:\krios\20190806\slot11_0329.tif 34 frames are being saved to U:\krios\20190806\slot11_0330.tif 34 frames are being saved to U:\krios\20190806\slot11_0331.tif Item 174: Stage: -794.70 646.27 70 Label: 172 174 Tick time from program start = 38366.948 Running script 17 at item 173 (-793.62, 643.92) Remaining time to fill dewars is 19500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.25 -0.30 (2nd move error 0.19 0.04) Finished aligning to item in 1 rounds -0.4 -1.0 unbinned pixels; 3.2 2.9 nm along two shift axes; 4.0 -1.3 nm on specimen axes Total_Shift = 2.163515 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 3.31, -1.13 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -9.1, 8.3 unbinned pixels, 0.22 nm/sec Measured defocus = -0.11 microns changed by -1.19 to target 34 frames are being saved to U:\krios\20190806\slot11_0332.tif 34 frames are being saved to U:\krios\20190806\slot11_0333.tif 34 frames are being saved to U:\krios\20190806\slot11_0334.tif Item 175: Stage: -793.62 643.92 70 Label: 173 175 Tick time from program start = 38478.068 Running script 17 at item 174 (-792.62, 641.60) Remaining time to fill dewars is 19380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.32 -0.27 (2nd move error 0.27 0.07) Finished aligning to item in 1 rounds -0.5 -5.5 unbinned pixels; 19.6 10.1 nm along two shift axes; 21.5 -0.8 nm on specimen axes Total_Shift = 11.068925 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 7.38, -6.26 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -4.1, 12.9 unbinned pixels, 0.24 nm/sec Measured defocus = -0.30 microns changed by -1.10 to target 34 frames are being saved to U:\krios\20190806\slot11_0335.tif 34 frames are being saved to U:\krios\20190806\slot11_0336.tif 34 frames are being saved to U:\krios\20190806\slot11_0337.tif Item 176: Stage: -792.62 641.60 70 Label: 174 176 Tick time from program start = 38593.196 Running script 17 at item 175 (-789.20, 634.71) Remaining time to fill dewars is 19320 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.16 -0.22 Disparity in stage position after aligning to target position: 0.08 -0.33 (2nd move error -0.09 -0.12) Finished aligning to item in 2 rounds -16.1 18.4 unbinned pixels; 88.7 30.3 nm along two shift axes; -68.2 -66.5 nm on specimen axes Total_Shift = 48.897916 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 25.63, -17.40 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -3.8, 3.8 unbinned pixels, 0.10 nm/sec Measured defocus = -0.96 microns changed by -0.54 to target 34 frames are being saved to U:\krios\20190806\slot11_0338.tif 34 frames are being saved to U:\krios\20190806\slot11_0339.tif 34 frames are being saved to U:\krios\20190806\slot11_0340.tif Item 177: Stage: -789.20 634.71 70 Label: 175 177 Tick time from program start = 38714.175 Running script 17 at item 176 (-788.13, 632.36) Remaining time to fill dewars is 19080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.20 -0.31 (2nd move error 0.03 -0.12) Finished aligning to item in 1 rounds -15.0 -1.8 unbinned pixels; 12.9 56.6 nm along two shift axes; 10.1 -57.8 nm on specimen axes Total_Shift = 30.143839 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 31.32, -19.46 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -0.9, 8.1 unbinned pixels, 0.15 nm/sec Measured defocus = -1.14 microns changed by -0.46 to target 34 frames are being saved to U:\krios\20190806\slot11_0341.tif 34 frames are being saved to U:\krios\20190806\slot11_0342.tif 34 frames are being saved to U:\krios\20190806\slot11_0343.tif Item 178: Stage: -788.13 632.36 70 Label: 176 178 Tick time from program start = 38825.341 Running script 17 at item 177 (-786.99, 630.00) Remaining time to fill dewars is 19020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.13 -0.30 (2nd move error -0.05 -0.10) Finished aligning to item in 1 rounds -12.8 9.4 unbinned pixels; 51.3 31.9 nm along two shift axes; -33.9 -51.8 nm on specimen axes Total_Shift = 31.768208 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 35.91, -21.44 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -4.6, 3.9 unbinned pixels, 0.11 nm/sec Measured defocus = -1.29 microns changed by -0.41 to target 34 frames are being saved to U:\krios\20190806\slot11_0344.tif 34 frames are being saved to U:\krios\20190806\slot11_0345.tif 34 frames are being saved to U:\krios\20190806\slot11_0346.tif Item 179: Stage: -786.99 630.00 70 Label: 177 179 Tick time from program start = 38937.288 Running script 17 at item 178 (-785.92, 627.68) Remaining time to fill dewars is 18900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.33 -0.26 (2nd move error 0.16 -0.06) Finished aligning to item in 1 rounds -6.9 -2.7 unbinned pixels; 1.0 28.8 nm along two shift axes; 12.0 -26.1 nm on specimen axes Total_Shift = 14.749793 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 35.83, -23.29 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -1.1, 8.9 unbinned pixels, 0.16 nm/sec Measured defocus = -1.32 microns changed by -0.48 to target 34 frames are being saved to U:\krios\20190806\slot11_0347.tif 34 frames are being saved to U:\krios\20190806\slot11_0348.tif 34 frames are being saved to U:\krios\20190806\slot11_0349.tif Item 180: Stage: -785.92 627.68 70 Label: 178 180 Tick time from program start = 39048.470 Running script 17 at item 179 (-784.85, 625.40) Remaining time to fill dewars is 18780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.26 -0.28 (2nd move error 0.09 -0.08) Finished aligning to item in 1 rounds -9.3 6.1 unbinned pixels; 34.4 24.3 nm along two shift axes; -21.6 -37.4 nm on specimen axes Total_Shift = 22.17586 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 41.53, -24.84 unbinned pixels, unfiltered CCC is -0.0008 The average drift between shots was -2.4, 6.2 unbinned pixels, 0.12 nm/sec Measured defocus = -1.50 microns changed by -0.40 to target 34 frames are being saved to U:\krios\20190806\slot11_0350.tif 34 frames are being saved to U:\krios\20190806\slot11_0351.tif 34 frames are being saved to U:\krios\20190806\slot11_0352.tif Item 181: Stage: -784.85 625.40 70 Label: 179 181 Tick time from program start = 39159.964 Running script 17 at item 180 (-782.44, 620.72) Remaining time to fill dewars is 18600 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.14 -0.29 Disparity in stage position after aligning to target position: 0.09 -0.31 (2nd move error -0.05 -0.05) Finished aligning to item in 2 rounds -5.6 11.3 unbinned pixels; 48.9 2.9 nm along two shift axes; -42.9 -24.0 nm on specimen axes Total_Shift = 25.214998 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 43.29, -24.76 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -3.7, 3.7 unbinned pixels, 0.09 nm/sec Measured defocus = -1.54 microns changed by -0.46 to target 34 frames are being saved to U:\krios\20190806\slot11_0353.tif 34 frames are being saved to U:\krios\20190806\slot11_0354.tif 34 frames are being saved to U:\krios\20190806\slot11_0355.tif Item 182: Stage: -782.44 620.72 70 Label: 180 182 Tick time from program start = 39280.568 Running script 17 at item 181 (-781.39, 618.33) Remaining time to fill dewars is 18480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.05 -0.28 (2nd move error -0.08 -0.01) Finished aligning to item in 1 rounds -3.6 15.4 unbinned pixels; 61.3 10.2 nm along two shift axes; -59.1 -17.3 nm on specimen axes Total_Shift = 31.630098 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 45.16, -26.50 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -7.4, 4.8 unbinned pixels, 0.16 nm/sec Measured defocus = -1.62 microns changed by -0.48 to target 34 frames are being saved to U:\krios\20190806\slot11_0356.tif 34 frames are being saved to U:\krios\20190806\slot11_0357.tif 34 frames are being saved to U:\krios\20190806\slot11_0358.tif Item 183: Stage: -781.39 618.33 70 Label: 181 183 Tick time from program start = 39392.031 Running script 17 at item 182 (-780.33, 616.05) Remaining time to fill dewars is 18420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.28 (2nd move error 0.04 -0.01) Finished aligning to item in 1 rounds -2.9 -2.4 unbinned pixels; 5.0 13.9 nm along two shift axes; 9.9 -10.7 nm on specimen axes Total_Shift = 7.455852 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 45.39, -27.96 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -3.3, 11.3 unbinned pixels, 0.21 nm/sec Measured defocus = -1.65 microns changed by -0.55 to target 34 frames are being saved to U:\krios\20190806\slot11_0359.tif 34 frames are being saved to U:\krios\20190806\slot11_0360.tif 34 frames are being saved to U:\krios\20190806\slot11_0361.tif Item 184: Stage: -780.33 616.05 70 Label: 182 184 Tick time from program start = 39503.478 Running script 17 at item 183 (-779.24, 613.71) Remaining time to fill dewars is 18300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.23 (2nd move error 0.09 0.04) Finished aligning to item in 1 rounds -0.1 0.1 unbinned pixels; 0.4 0.4 nm along two shift axes; -0.3 -0.6 nm on specimen axes Total_Shift = 0.312514 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 43.42, -27.01 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was -8.2, 9.7 unbinned pixels, 0.22 nm/sec Measured defocus = -1.58 microns changed by -0.72 to target 34 frames are being saved to U:\krios\20190806\slot11_0362.tif 34 frames are being saved to U:\krios\20190806\slot11_0363.tif 34 frames are being saved to U:\krios\20190806\slot11_0364.tif Item 185: Stage: -779.24 613.71 70 Label: 183 185 Tick time from program start = 39614.909 Running script 17 at item 184 (-778.07, 611.38) Remaining time to fill dewars is 18120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.24 -0.22 (2nd move error 0.10 0.04) Finished aligning to item in 1 rounds -0.4 -0.2 unbinned pixels; 0.2 1.7 nm along two shift axes; 0.8 -1.5 nm on specimen axes Total_Shift = 0.861827 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 45.74, -27.42 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -9.7, 11.3 unbinned pixels, 0.27 nm/sec Measured defocus = -1.65 microns changed by -0.75 to target 34 frames are being saved to U:\krios\20190806\slot11_0365.tif 34 frames are being saved to U:\krios\20190806\slot11_0366.tif 34 frames are being saved to U:\krios\20190806\slot11_0367.tif Item 186: Stage: -778.07 611.38 70 Label: 184 186 Tick time from program start = 39726.731 Running script 17 at item 185 (-776.94, 609.00) Remaining time to fill dewars is 18000 Dewars are NOT busy filling Trimming A by 20 55 20 20, reference by 20 20 20 20 Trimming A by 20 55 20 20, reference by 20 20 20 20 Trimming A by 20 55 20 20, reference by 20 20 20 20 Trimming A by 20 55 20 20, reference by 20 50 20 20 Trimming A by 20 55 20 20, reference by 20 60 20 20 Trimming A by 20 55 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.08 -0.31 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.12 -0.18 (2nd move error 0.03 0.10) Finished aligning to item in 2 rounds -0.4 1.0 unbinned pixels; 4.1 0.0 nm along two shift axes; -3.7 -1.8 nm on specimen axes Total_Shift = 2.118662 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 43.61, -27.28 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -9.7, 12.9 unbinned pixels, 0.29 nm/sec Measured defocus = -1.59 microns changed by -0.91 to target 34 frames are being saved to U:\krios\20190806\slot11_0368.tif 34 frames are being saved to U:\krios\20190806\slot11_0369.tif 34 frames are being saved to U:\krios\20190806\slot11_0370.tif Item 187: Stage: -776.94 609.00 70 Label: 185 187 Tick time from program start = 39844.886 Running script 17 at item 186 (-775.91, 606.69) Remaining time to fill dewars is 17940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.14 -0.18 (2nd move error 0.06 0.11) Finished aligning to item in 1 rounds 1.0 0.9 unbinned pixels; 1.8 5.0 nm along two shift axes; -3.6 3.9 nm on specimen axes Total_Shift = 2.705687 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 43.14, -27.50 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -8.2, 11.4 unbinned pixels, 0.25 nm/sec Measured defocus = -1.58 microns changed by 0.28 to target 34 frames are being saved to U:\krios\20190806\slot11_0371.tif 34 frames are being saved to U:\krios\20190806\slot11_0372.tif 34 frames are being saved to U:\krios\20190806\slot11_0373.tif Item 188: Stage: -775.91 606.69 70 Label: 186 188 Tick time from program start = 39956.302 Running script 17 at item 187 (-774.81, 604.48) Remaining time to fill dewars is 17700 Dewars are NOT busy filling Trimming A by 20 100 65 20, reference by 20 20 20 20 Trimming A by 20 100 65 20, reference by 20 20 20 20 Trimming A by 20 100 65 20, reference by 20 60 20 20 Trimming A by 20 100 65 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.23 Trimming A by 20 65 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to target position: 0.09 -0.35 (2nd move error 0.02 -0.14) Finished aligning to item in 2 rounds Trimming A by 20 70 20 20, reference by 20 65 20 20 -17.9 -1.6 unbinned pixels; 17.4 66.8 nm along two shift axes; 10.1 -69.2 nm on specimen axes Total_Shift = 35.878842 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 2.28, 1.20 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.1, 5.4 unbinned pixels, 0.13 nm/sec Measured defocus = -0.04 microns changed by -1.26 to target 34 frames are being saved to U:\krios\20190806\slot11_0374.tif 34 frames are being saved to U:\krios\20190806\slot11_0375.tif 34 frames are being saved to U:\krios\20190806\slot11_0376.tif Item 189: Stage: -774.81 604.48 70 Label: 187 189 Tick time from program start = 40080.323 Running script 17 at item 188 (-777.06, 603.28) Remaining time to fill dewars is 17580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 94 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.20 -0.24 (2nd move error 0.11 0.04) Finished aligning to item in 1 rounds Trimming A by 20 90 50 20, reference by 20 94 20 20 -0.3 1.7 unbinned pixels; 6.5 1.4 nm along two shift axes; -6.4 -1.6 nm on specimen axes Total_Shift = 3.375424 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -193.74, -123.19 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was 185.8, 128.3 unbinned pixels, 4.00 nm/sec Measured defocus = 3.19 microns changed by -4.59 to target Tilt-induced shift is 127.09, -79.16 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -0.3, 3.1 unbinned pixels, 0.05 nm/sec Measured defocus = -4.64 microns changed by 3.24 to target Tilt-induced shift is 81.21, -50.67 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -0.3, 2.5 unbinned pixels, 0.04 nm/sec Measured defocus = -2.96 microns changed by 1.56 to target Tilt-induced shift is 42.25, -26.68 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -0.0, 1.5 unbinned pixels, 0.03 nm/sec Measured defocus = -1.54 microns changed by 0.14 to target 34 frames are being saved to U:\krios\20190806\slot11_0377.tif 34 frames are being saved to U:\krios\20190806\slot11_0378.tif 34 frames are being saved to U:\krios\20190806\slot11_0379.tif Item 190: Stage: -777.06 603.28 70 Label: 188 190 Tick time from program start = 40220.178 Running script 17 at item 189 (-778.13, 605.60) Remaining time to fill dewars is 17340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.17 -0.24 (2nd move error 0.08 0.04) Finished aligning to item in 1 rounds -0.3 -1.3 unbinned pixels; 4.6 3.0 nm along two shift axes; 5.3 -0.8 nm on specimen axes Total_Shift = 2.749909 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 9.29, -5.41 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -10.7, 8.3 unbinned pixels, 0.24 nm/sec Measured defocus = -0.33 microns changed by -1.17 to target 34 frames are being saved to U:\krios\20190806\slot11_0380.tif 34 frames are being saved to U:\krios\20190806\slot11_0381.tif 34 frames are being saved to U:\krios\20190806\slot11_0382.tif Item 191: Stage: -778.13 605.60 70 Label: 189 191 Tick time from program start = 40334.963 Running script 17 at item 190 (-779.18, 607.97) Remaining time to fill dewars is 17280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.17 -0.27 (2nd move error 0.09 0.01) Finished aligning to item in 1 rounds 1.8 -1.3 unbinned pixels; 7.1 4.3 nm along two shift axes; 4.8 7.1 nm on specimen axes Total_Shift = 4.39813 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 14.08, -9.24 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -10.3, 10.3 unbinned pixels, 0.26 nm/sec Measured defocus = -0.52 microns changed by -1.08 to target 34 frames are being saved to U:\krios\20190806\slot11_0383.tif 34 frames are being saved to U:\krios\20190806\slot11_0384.tif 34 frames are being saved to U:\krios\20190806\slot11_0385.tif Item 192: Stage: -779.18 607.97 70 Label: 190 192 Tick time from program start = 40446.722 Running script 17 at item 191 (-780.24, 610.29) Remaining time to fill dewars is 17160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.14 -0.26 (2nd move error 0.05 0.02) Finished aligning to item in 1 rounds 0.5 -3.3 unbinned pixels; 13.0 3.1 nm along two shift axes; 12.9 2.8 nm on specimen axes Total_Shift = 6.768821 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 20.63, -13.89 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -11.3, 12.0 unbinned pixels, 0.29 nm/sec Measured defocus = -0.77 microns changed by -0.93 to target 34 frames are being saved to U:\krios\20190806\slot11_0386.tif 34 frames are being saved to U:\krios\20190806\slot11_0387.tif 34 frames are being saved to U:\krios\20190806\slot11_0388.tif Item 193: Stage: -780.24 610.29 70 Label: 191 193 Tick time from program start = 40558.341 Running script 17 at item 192 (-781.52, 612.68) Remaining time to fill dewars is 17040 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.31 Disparity in stage position after aligning to target position: 0.19 -0.29 (2nd move error 0.03 -0.01) Finished aligning to item in 2 rounds -2.8 -0.9 unbinned pixels; 0.4 11.6 nm along two shift axes; 4.1 -10.9 nm on specimen axes Total_Shift = 5.962608 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 24.40, -14.59 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -6.1, 10.2 unbinned pixels, 0.21 nm/sec Measured defocus = -0.88 microns changed by -0.92 to target 34 frames are being saved to U:\krios\20190806\slot11_0389.tif 34 frames are being saved to U:\krios\20190806\slot11_0390.tif 34 frames are being saved to U:\krios\20190806\slot11_0391.tif Item 194: Stage: -781.52 612.68 70 Label: 192 194 Tick time from program start = 40679.304 Running script 17 at item 193 (-782.58, 614.99) Remaining time to fill dewars is 16920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.21 -0.30 (2nd move error 0.06 -0.02) Finished aligning to item in 1 rounds -3.2 -4.0 unbinned pixels; 10.7 17.5 nm along two shift axes; 16.3 -11.5 nm on specimen axes Total_Shift = 10.252442 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 31.00, -19.51 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -5.7, 10.7 unbinned pixels, 0.21 nm/sec Measured defocus = -1.13 microns changed by -0.77 to target 34 frames are being saved to U:\krios\20190806\slot11_0392.tif 34 frames are being saved to U:\krios\20190806\slot11_0393.tif 34 frames are being saved to U:\krios\20190806\slot11_0394.tif Item 195: Stage: -782.58 614.99 70 Label: 193 195 Tick time from program start = 40791.734 Running script 17 at item 194 (-783.61, 617.31) Remaining time to fill dewars is 16800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.10 -0.32 (2nd move error -0.06 -0.03) Finished aligning to item in 1 rounds -4.4 13.3 unbinned pixels; 54.8 4.3 nm along two shift axes; -51.0 -19.9 nm on specimen axes Total_Shift = 28.09191 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 38.52, -24.07 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -7.6, 4.4 unbinned pixels, 0.16 nm/sec Measured defocus = -1.40 microns changed by -0.60 to target 34 frames are being saved to U:\krios\20190806\slot11_0395.tif 34 frames are being saved to U:\krios\20190806\slot11_0396.tif 34 frames are being saved to U:\krios\20190806\slot11_0397.tif Item 196: Stage: -783.61 617.31 70 Label: 194 196 Tick time from program start = 40903.337 Running script 17 at item 195 (-784.65, 619.65) Remaining time to fill dewars is 16620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.02 -0.32 (2nd move error -0.13 -0.02) Finished aligning to item in 1 rounds -4.0 23.1 unbinned pixels; 90.0 20.5 nm along two shift axes; -88.9 -20.3 nm on specimen axes Total_Shift = 46.834198 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 42.02, -26.81 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -4.0, 4.1 unbinned pixels, 0.10 nm/sec Measured defocus = -1.54 microns changed by -0.56 to target 34 frames are being saved to U:\krios\20190806\slot11_0398.tif 34 frames are being saved to U:\krios\20190806\slot11_0399.tif 34 frames are being saved to U:\krios\20190806\slot11_0400.tif Item 197: Stage: -784.65 619.65 70 Label: 195 197 Tick time from program start = 41015.393 Running script 17 at item 196 (-785.78, 622.02) Remaining time to fill dewars is 16500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.11 -0.34 (2nd move error -0.03 -0.05) Finished aligning to item in 1 rounds -7.0 8.0 unbinned pixels; 38.4 13.1 nm along two shift axes; -29.5 -28.8 nm on specimen axes Total_Shift = 21.165335 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 48.18, -30.76 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.1, 4.6 unbinned pixels, 0.11 nm/sec Measured defocus = -1.77 microns changed by -0.43 to target 34 frames are being saved to U:\krios\20190806\slot11_0401.tif 34 frames are being saved to U:\krios\20190806\slot11_0402.tif 34 frames are being saved to U:\krios\20190806\slot11_0403.tif Item 198: Stage: -785.78 622.02 70 Label: 196 198 Tick time from program start = 41126.902 Running script 17 at item 197 (-787.12, 624.41) Remaining time to fill dewars is 16320 Dewars are NOT busy filling Disparity in stage position after aligning to center of map frame: 0.21 -0.24 Disparity in stage position after aligning to target position: 0.18 -0.33 (2nd move error -0.04 -0.12) Finished aligning to item in 2 rounds -15.0 8.6 unbinned pixels; 51.3 41.1 nm along two shift axes; -30.3 -60.2 nm on specimen axes Total_Shift = 34.603243 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 52.20, -30.56 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -3.9, 3.9 unbinned pixels, 0.10 nm/sec Measured defocus = -1.87 microns changed by -0.43 to target 34 frames are being saved to U:\krios\20190806\slot11_0404.tif 34 frames are being saved to U:\krios\20190806\slot11_0405.tif 34 frames are being saved to U:\krios\20190806\slot11_0406.tif Item 199: Stage: -787.12 624.41 70 Label: 197 199 Tick time from program start = 41245.525 Running script 17 at item 198 (-788.17, 626.69) Remaining time to fill dewars is 16260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.28 -0.34 (2nd move error 0.06 -0.11) Finished aligning to item in 1 rounds -12.7 0.9 unbinned pixels; 20.0 44.5 nm along two shift axes; -0.9 -49.7 nm on specimen axes Total_Shift = 25.538928 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 53.10, -34.50 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was 0.6, 8.7 unbinned pixels, 0.15 nm/sec Measured defocus = -1.96 microns changed by -0.44 to target 34 frames are being saved to U:\krios\20190806\slot11_0407.tif 34 frames are being saved to U:\krios\20190806\slot11_0408.tif 34 frames are being saved to U:\krios\20190806\slot11_0409.tif Item 200: Stage: -788.17 626.69 70 Label: 198 200 Tick time from program start = 41357.753 Running script 17 at item 199 (-789.34, 628.92) Remaining time to fill dewars is 16140 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.04 -0.34 Disparity in stage position after aligning to target position: 0.25 -0.22 (2nd move error 0.21 0.09) Finished aligning to item in 2 rounds -2.7 -2.4 unbinned pixels; 5.4 13.5 nm along two shift axes; 10.1 -10.1 nm on specimen axes Total_Shift = 7.324431 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 55.40, -36.87 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -3.5, 12.5 unbinned pixels, 0.23 nm/sec Measured defocus = -2.06 microns changed by -0.44 to target 34 frames are being saved to U:\krios\20190806\slot11_0410.tif 34 frames are being saved to U:\krios\20190806\slot11_0411.tif 34 frames are being saved to U:\krios\20190806\slot11_0412.tif Item 201: Stage: -789.34 628.92 70 Label: 199 201 Tick time from program start = 41478.887 Running script 17 at item 200 (-790.34, 631.23) Remaining time to fill dewars is 16020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.16 -0.27 (2nd move error 0.13 0.04) Finished aligning to item in 1 rounds -1.2 -7.0 unbinned pixels; 24.1 14.8 nm along two shift axes; 27.4 -3.2 nm on specimen axes Total_Shift = 14.150628 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 19.24, -14.00 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -4.9, 9.4 unbinned pixels, 0.19 nm/sec Measured defocus = -0.73 microns changed by -0.57 to target 34 frames are being saved to U:\krios\20190806\slot11_0413.tif 34 frames are being saved to U:\krios\20190806\slot11_0414.tif 34 frames are being saved to U:\krios\20190806\slot11_0415.tif Item 202: Stage: -790.34 631.23 70 Label: 200 202 Tick time from program start = 41591.083 Running script 17 at item 201 (-791.42, 633.55) Remaining time to fill dewars is 15900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.21 -0.32 (2nd move error 0.17 -0.00) Finished aligning to item in 1 rounds -0.1 3.0 unbinned pixels; 11.3 4.1 nm along two shift axes; -11.8 -1.1 nm on specimen axes Total_Shift = 6.061534 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 19.66, -14.73 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -3.4, 9.2 unbinned pixels, 0.17 nm/sec Measured defocus = -0.76 microns changed by -0.64 to target 34 frames are being saved to U:\krios\20190806\slot11_0416.tif 34 frames are being saved to U:\krios\20190806\slot11_0417.tif 34 frames are being saved to U:\krios\20190806\slot11_0418.tif Item 203: Stage: -791.42 633.55 70 Label: 201 203 Tick time from program start = 41703.108 Running script 17 at item 202 (-792.45, 635.89) Remaining time to fill dewars is 15660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.30 (2nd move error 0.11 0.02) Finished aligning to item in 1 rounds 1.9 -4.4 unbinned pixels; 18.7 0.3 nm along two shift axes; 16.7 8.4 nm on specimen axes Total_Shift = 9.597176 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 17.44, -15.46 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -1.9, 13.1 unbinned pixels, 0.24 nm/sec Measured defocus = -0.71 microns changed by -0.79 to target 34 frames are being saved to U:\krios\20190806\slot11_0419.tif 34 frames are being saved to U:\krios\20190806\slot11_0420.tif 34 frames are being saved to U:\krios\20190806\slot11_0421.tif Item 204: Stage: -792.45 635.89 70 Label: 202 204 Tick time from program start = 41814.882 Running script 17 at item 203 (-793.80, 638.30) Remaining time to fill dewars is 15600 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.05 -0.36 Disparity in stage position after aligning to target position: 0.29 -0.31 (2nd move error 0.23 0.02) Finished aligning to item in 2 rounds 1.6 1.7 unbinned pixels; 4.0 8.2 nm along two shift axes; -6.8 5.8 nm on specimen axes Total_Shift = 4.595888 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 13.31, -9.82 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -6.5, 11.1 unbinned pixels, 0.23 nm/sec Measured defocus = -0.51 microns changed by -1.09 to target 34 frames are being saved to U:\krios\20190806\slot11_0422.tif 34 frames are being saved to U:\krios\20190806\slot11_0423.tif 34 frames are being saved to U:\krios\20190806\slot11_0424.tif Item 205: Stage: -793.80 638.30 70 Label: 203 205 Tick time from program start = 41936.048 Running script 17 at item 204 (-794.77, 640.58) Remaining time to fill dewars is 15420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.27 -0.33 (2nd move error 0.21 0.01) Finished aligning to item in 1 rounds 1.7 -0.3 unbinned pixels; 3.4 5.7 nm along two shift axes; 0.9 6.7 nm on specimen axes Total_Shift = 3.480328 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 12.34, -6.71 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -8.0, 7.6 unbinned pixels, 0.20 nm/sec Measured defocus = -0.43 microns changed by -1.27 to target 34 frames are being saved to U:\krios\20190806\slot11_0425.tif 34 frames are being saved to U:\krios\20190806\slot11_0426.tif 34 frames are being saved to U:\krios\20190806\slot11_0427.tif Item 206: Stage: -794.77 640.58 70 Label: 204 206 Tick time from program start = 42048.010 Running script 17 at item 205 (-795.80, 642.88) Remaining time to fill dewars is 15300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.21 -0.35 (2nd move error 0.15 -0.01) Finished aligning to item in 1 rounds -2.3 -2.3 unbinned pixels; 5.4 11.5 nm along two shift axes; 9.3 -8.3 nm on specimen axes Total_Shift = 6.408541 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 12.53, -8.02 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -3.2, 7.4 unbinned pixels, 0.14 nm/sec Measured defocus = -0.46 microns changed by -1.34 to target 34 frames are being saved to U:\krios\20190806\slot11_0428.tif 34 frames are being saved to U:\krios\20190806\slot11_0429.tif 34 frames are being saved to U:\krios\20190806\slot11_0430.tif Item 207: Stage: -795.80 642.88 70 Label: 205 207 Tick time from program start = 42163.451 Running script 17 at item 206 (-796.91, 645.25) Remaining time to fill dewars is 15120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.35 (2nd move error 0.08 -0.01) Finished aligning to item in 1 rounds -2.3 -0.9 unbinned pixels; 0.1 9.8 nm along two shift axes; 3.9 -9.0 nm on specimen axes Total_Shift = 5.004154 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 16.39, -9.83 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.0, 7.3 unbinned pixels, 0.16 nm/sec Measured defocus = -0.59 microns changed by -1.31 to target 34 frames are being saved to U:\krios\20190806\slot11_0431.tif 34 frames are being saved to U:\krios\20190806\slot11_0432.tif 34 frames are being saved to U:\krios\20190806\slot11_0433.tif Item 208: Stage: -796.91 645.25 70 Label: 206 208 Tick time from program start = 42274.430 Running script 17 at item 207 (-797.98, 647.63) Remaining time to fill dewars is 15060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 45 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.10 -0.37 (2nd move error 0.05 -0.03) Finished aligning to item in 1 rounds Trimming A by 45 20 20 20, reference by 45 20 20 20 -5.4 -3.3 unbinned pixels; 5.3 24.4 nm along two shift axes; 14.1 -20.2 nm on specimen axes Total_Shift = 12.660619 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 17.84, -11.35 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -2.4, 6.8 unbinned pixels, 0.13 nm/sec Measured defocus = -0.65 microns changed by -1.35 to target 34 frames are being saved to U:\krios\20190806\slot11_0434.tif 34 frames are being saved to U:\krios\20190806\slot11_0435.tif 34 frames are being saved to U:\krios\20190806\slot11_0436.tif Item 209: Stage: -797.98 647.63 70 Label: 207 209 Tick time from program start = 42389.808 Running script 17 at item 208 (-801.33, 648.90) Remaining time to fill dewars is 14880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 100 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.00 -0.40 (2nd move error -0.06 -0.06) Finished aligning to item in 1 rounds Trimming A by 99 20 20 20, reference by 100 20 20 20 -8.2 11.2 unbinned pixels; 51.7 12.6 nm along two shift axes; -41.8 -34.1 nm on specimen axes Total_Shift = 27.678901 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 55.03, -35.76 unbinned pixels, unfiltered CCC is -0.0008 The average drift between shots was -2.6, 3.8 unbinned pixels, 0.08 nm/sec Measured defocus = -2.03 microns changed by -0.07 to target 34 frames are being saved to U:\krios\20190806\slot11_0437.tif 34 frames are being saved to U:\krios\20190806\slot11_0438.tif 34 frames are being saved to U:\krios\20190806\slot11_0439.tif Item 210: Stage: -801.33 648.90 70 Label: 208 210 Tick time from program start = 42501.879 Running script 17 at item 209 (-800.23, 646.53) Remaining time to fill dewars is 14820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.14 -0.41 (2nd move error 0.08 -0.07) Finished aligning to item in 1 rounds -9.4 -4.9 unbinned pixels; 5.8 41.4 nm along two shift axes; 21.1 -35.7 nm on specimen axes Total_Shift = 21.28315 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 28.11, -15.55 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -1.5, 7.5 unbinned pixels, 0.14 nm/sec Measured defocus = -0.99 microns changed by -1.21 to target 34 frames are being saved to U:\krios\20190806\slot11_0440.tif 34 frames are being saved to U:\krios\20190806\slot11_0441.tif 34 frames are being saved to U:\krios\20190806\slot11_0442.tif Item 211: Stage: -800.23 646.53 70 Label: 209 211 Tick time from program start = 42613.639 Running script 17 at item 210 (-799.15, 644.15) Remaining time to fill dewars is 14700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.22 -0.37 (2nd move error 0.16 -0.03) Finished aligning to item in 1 rounds -1.9 -0.0 unbinned pixels; 2.4 7.0 nm along two shift axes; 0.5 -7.5 nm on specimen axes Total_Shift = 3.85622 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 63.90, -43.19 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was 1.0, 9.3 unbinned pixels, 0.17 nm/sec Measured defocus = -2.38 microns changed by 0.08 to target 34 frames are being saved to U:\krios\20190806\slot11_0443.tif 34 frames are being saved to U:\krios\20190806\slot11_0444.tif 34 frames are being saved to U:\krios\20190806\slot11_0445.tif Item 212: Stage: -799.15 644.15 70 Label: 210 212 Tick time from program start = 42724.898 Running script 17 at item 211 (-798.09, 641.78) Remaining time to fill dewars is 14520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.21 -0.35 (2nd move error 0.15 -0.01) Finished aligning to item in 1 rounds -1.1 0.0 unbinned pixels; 1.6 3.9 nm along two shift axes; 0.1 -4.2 nm on specimen axes Total_Shift = 2.175354 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 32.48, -19.08 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -2.8, 7.4 unbinned pixels, 0.14 nm/sec Measured defocus = -1.16 microns changed by -1.24 to target 34 frames are being saved to U:\krios\20190806\slot11_0446.tif 34 frames are being saved to U:\krios\20190806\slot11_0447.tif 34 frames are being saved to U:\krios\20190806\slot11_0448.tif Item 213: Stage: -798.09 641.78 70 Label: 211 213 Tick time from program start = 42835.800 Running script 17 at item 212 (-797.01, 639.53) Remaining time to fill dewars is 14400 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.16 -0.31 Disparity in stage position after aligning to target position: 0.27 -0.35 (2nd move error 0.10 -0.06) Finished aligning to item in 2 rounds -5.3 2.2 unbinned pixels; 14.9 16.0 nm along two shift axes; -7.3 -21.2 nm on specimen axes Total_Shift = 11.513354 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 67.77, -43.56 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was 0.3, 6.7 unbinned pixels, 0.12 nm/sec Measured defocus = -2.49 microns changed by -0.01 to target 34 frames are being saved to U:\krios\20190806\slot11_0449.tif 34 frames are being saved to U:\krios\20190806\slot11_0450.tif 34 frames are being saved to U:\krios\20190806\slot11_0451.tif Item 214: Stage: -797.01 639.53 70 Label: 212 214 Tick time from program start = 42953.846 Running script 17 at item 213 (-795.75, 637.17) Remaining time to fill dewars is 14220 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.21 -0.33 Disparity in stage position after aligning to target position: 0.12 -0.28 (2nd move error -0.09 0.03) Finished aligning to item in 2 rounds -0.7 18.1 unbinned pixels; 67.6 24.6 nm along two shift axes; -70.3 -6.7 nm on specimen axes Total_Shift = 36.269962 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Using peak at -5.2,-2.0, rejecting 1.17 times higher peak at -0.1,0.2 Tilt-induced shift is 1.90, -1.33 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -8.5, 2.6 unbinned pixels, 0.16 nm/sec Measured defocus = -0.07 microns changed by -1.23 to target 34 frames are being saved to U:\krios\20190806\slot11_0452.tif 34 frames are being saved to U:\krios\20190806\slot11_0453.tif 34 frames are being saved to U:\krios\20190806\slot11_0454.tif Item 215: Stage: -795.75 637.17 70 Label: 213 215 Tick time from program start = 43072.219 Running script 17 at item 214 (-794.70, 634.80) Remaining time to fill dewars is 14160 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.29 (2nd move error -0.06 0.02) Finished aligning to item in 1 rounds 1.4 12.3 unbinned pixels; 43.4 23.5 nm along two shift axes; -48.1 2.8 nm on specimen axes Total_Shift = 24.746567 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 7.74, -6.81 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -4.4, 7.5 unbinned pixels, 0.16 nm/sec Measured defocus = -0.31 microns changed by -0.99 to target 34 frames are being saved to U:\krios\20190806\slot11_0455.tif 34 frames are being saved to U:\krios\20190806\slot11_0456.tif 34 frames are being saved to U:\krios\20190806\slot11_0457.tif Item 216: Stage: -794.70 634.80 70 Label: 214 216 Tick time from program start = 43200.858 Running script 17 at item 215 (-793.63, 632.45) Remaining time to fill dewars is 14040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.26 (2nd move error -0.06 0.05) Finished aligning to item in 1 rounds -2.7 15.1 unbinned pixels; 59.1 12.9 nm along two shift axes; -58.1 -13.8 nm on specimen axes Total_Shift = 30.672827 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 15.86, -12.59 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -1.4, 6.1 unbinned pixels, 0.11 nm/sec Measured defocus = -0.62 microns changed by -0.78 to target 34 frames are being saved to U:\krios\20190806\slot11_0458.tif 34 frames are being saved to U:\krios\20190806\slot11_0459.tif 34 frames are being saved to U:\krios\20190806\slot11_0460.tif Item 217: Stage: -793.63 632.45 70 Label: 215 217 Tick time from program start = 43312.897 Running script 17 at item 216 (-792.57, 630.12) Remaining time to fill dewars is 13920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.27 -0.27 (2nd move error 0.06 0.04) Finished aligning to item in 1 rounds -0.5 -0.2 unbinned pixels; 0.2 2.1 nm along two shift axes; 0.9 -1.8 nm on specimen axes Total_Shift = 1.063746 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 23.59, -15.80 unbinned pixels, unfiltered CCC is -0.0008 The average drift between shots was -5.2, 9.5 unbinned pixels, 0.19 nm/sec Measured defocus = -0.88 microns changed by -0.62 to target 34 frames are being saved to U:\krios\20190806\slot11_0461.tif 34 frames are being saved to U:\krios\20190806\slot11_0462.tif 34 frames are being saved to U:\krios\20190806\slot11_0463.tif Item 218: Stage: -792.57 630.12 70 Label: 216 218 Tick time from program start = 43424.189 Running script 17 at item 217 (-791.57, 627.82) Remaining time to fill dewars is 13680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.31 -0.23 (2nd move error 0.09 0.08) Finished aligning to item in 1 rounds 0.0 2.4 unbinned pixels; 8.8 3.8 nm along two shift axes; -9.4 -0.3 nm on specimen axes Total_Shift = 4.828179 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 31.43, -17.57 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -7.1, 9.0 unbinned pixels, 0.20 nm/sec Measured defocus = -1.11 microns changed by -0.49 to target 34 frames are being saved to U:\krios\20190806\slot11_0464.tif 34 frames are being saved to U:\krios\20190806\slot11_0465.tif 34 frames are being saved to U:\krios\20190806\slot11_0466.tif Item 219: Stage: -791.57 627.82 70 Label: 217 219 Tick time from program start = 43535.714 Running script 17 at item 218 (-790.51, 625.53) Remaining time to fill dewars is 13560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.24 -0.24 (2nd move error 0.02 0.07) Finished aligning to item in 1 rounds 0.2 -1.8 unbinned pixels; 6.9 2.2 nm along two shift axes; 7.1 1.0 nm on specimen axes Total_Shift = 3.65858 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 32.82, -21.94 unbinned pixels, unfiltered CCC is -0.0011 The average drift between shots was -8.3, 10.0 unbinned pixels, 0.23 nm/sec Measured defocus = -1.22 microns changed by -0.48 to target 34 frames are being saved to U:\krios\20190806\slot11_0467.tif 34 frames are being saved to U:\krios\20190806\slot11_0468.tif 34 frames are being saved to U:\krios\20190806\slot11_0469.tif Item 220: Stage: -790.51 625.53 70 Label: 218 220 Tick time from program start = 43650.172 Running script 17 at item 219 (-789.26, 623.24) Remaining time to fill dewars is 13440 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: 0.11 -0.35 Disparity in stage position after aligning to target position: 0.11 -0.17 (2nd move error -0.01 0.16) Finished aligning to item in 2 rounds 3.6 3.4 unbinned pixels; 7.7 18.1 nm along two shift axes; -13.9 13.3 nm on specimen axes Total_Shift = 9.874775 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 36.94, -22.21 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -10.8, 6.0 unbinned pixels, 0.22 nm/sec Measured defocus = -1.33 microns changed by -0.47 to target 34 frames are being saved to U:\krios\20190806\slot11_0470.tif 34 frames are being saved to U:\krios\20190806\slot11_0471.tif 34 frames are being saved to U:\krios\20190806\slot11_0472.tif Item 221: Stage: -789.26 623.24 70 Label: 219 221 Tick time from program start = 43769.575 Running script 17 at item 220 (-788.02, 620.93) Remaining time to fill dewars is 13320 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.23 Disparity in stage position after aligning to target position: -0.02 -0.36 (2nd move error -0.08 -0.15) Finished aligning to item in 2 rounds -20.8 15.3 unbinned pixels; 83.4 51.9 nm along two shift axes; -55.2 -84.2 nm on specimen axes Total_Shift = 51.668705 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 36.30, -23.71 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -4.2, 3.3 unbinned pixels, 0.10 nm/sec Measured defocus = -1.34 microns changed by -0.56 to target 34 frames are being saved to U:\krios\20190806\slot11_0473.tif 34 frames are being saved to U:\krios\20190806\slot11_0474.tif 34 frames are being saved to U:\krios\20190806\slot11_0475.tif Item 222: Stage: -788.02 620.93 70 Label: 220 222 Tick time from program start = 43890.866 Running script 17 at item 221 (-786.95, 618.58) Remaining time to fill dewars is 13200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.05 -0.35 (2nd move error -0.11 -0.14) Finished aligning to item in 1 rounds -17.9 23.0 unbinned pixels; 108.0 29.8 nm along two shift axes; -85.8 -74.5 nm on specimen axes Total_Shift = 58.317324 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 40.89, -21.46 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -4.6, 3.1 unbinned pixels, 0.10 nm/sec Measured defocus = -1.42 microns changed by -0.58 to target 34 frames are being saved to U:\krios\20190806\slot11_0476.tif 34 frames are being saved to U:\krios\20190806\slot11_0477.tif 34 frames are being saved to U:\krios\20190806\slot11_0478.tif Item 223: Stage: -786.95 618.58 70 Label: 221 223 Tick time from program start = 44002.718 Running script 17 at item 222 (-785.84, 616.24) Remaining time to fill dewars is 13140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.36 (2nd move error 0.09 -0.15) Finished aligning to item in 1 rounds -16.4 -3.1 unbinned pixels; 9.9 63.7 nm along two shift axes; 15.6 -63.1 nm on specimen axes Total_Shift = 33.365176 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 38.57, -24.34 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -4.2, 9.1 unbinned pixels, 0.18 nm/sec Measured defocus = -1.41 microns changed by -0.69 to target 34 frames are being saved to U:\krios\20190806\slot11_0479.tif 34 frames are being saved to U:\krios\20190806\slot11_0480.tif 34 frames are being saved to U:\krios\20190806\slot11_0481.tif Item 224: Stage: -785.84 616.24 70 Label: 222 224 Tick time from program start = 44114.243 Running script 17 at item 223 (-784.74, 613.89) Remaining time to fill dewars is 12960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.09 -0.31 (2nd move error 0.03 -0.09) Finished aligning to item in 1 rounds -10.1 1.2 unbinned pixels; 17.6 34.5 nm along two shift axes; -2.6 -39.5 nm on specimen axes Total_Shift = 20.313595 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 36.29, -23.04 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.9, 7.4 unbinned pixels, 0.15 nm/sec Measured defocus = -1.33 microns changed by -0.87 to target 34 frames are being saved to U:\krios\20190806\slot11_0482.tif 34 frames are being saved to U:\krios\20190806\slot11_0483.tif 34 frames are being saved to U:\krios\20190806\slot11_0484.tif Item 225: Stage: -784.74 613.89 70 Label: 223 225 Tick time from program start = 44225.597 Running script 17 at item 224 (-783.71, 611.67) Remaining time to fill dewars is 12780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.08 -0.31 (2nd move error 0.02 -0.09) Finished aligning to item in 1 rounds -9.5 -1.8 unbinned pixels; 5.7 37.0 nm along two shift axes; 9.1 -36.6 nm on specimen axes Total_Shift = 19.374368 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 37.02, -23.13 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -2.4, 7.8 unbinned pixels, 0.14 nm/sec Measured defocus = -1.35 microns changed by -0.95 to target 34 frames are being saved to U:\krios\20190806\slot11_0485.tif 34 frames are being saved to U:\krios\20190806\slot11_0486.tif 34 frames are being saved to U:\krios\20190806\slot11_0487.tif Item 226: Stage: -783.71 611.67 70 Label: 224 226 Tick time from program start = 44336.935 Running script 17 at item 225 (-782.49, 609.30) Remaining time to fill dewars is 12660 Dewars are NOT busy filling Trimming A by 20 60 20 20, reference by 20 20 20 20 Trimming A by 20 60 20 20, reference by 20 20 20 20 Trimming A by 20 60 20 20, reference by 20 20 20 20 Trimming A by 20 60 20 20, reference by 20 50 20 20 Trimming A by 20 60 20 20, reference by 20 60 20 20 Trimming A by 20 60 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.06 -0.26 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.07 -0.27 (2nd move error 0.01 -0.03) Finished aligning to item in 2 rounds -4.5 -0.8 unbinned pixels; 2.9 17.6 nm along two shift axes; 4.2 -17.5 nm on specimen axes Total_Shift = 9.228115 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 72.58, -45.19 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -2.3, 7.9 unbinned pixels, 0.15 nm/sec Measured defocus = -2.64 microns changed by 0.24 to target 34 frames are being saved to U:\krios\20190806\slot11_0488.tif 34 frames are being saved to U:\krios\20190806\slot11_0489.tif 34 frames are being saved to U:\krios\20190806\slot11_0490.tif Item 227: Stage: -782.49 609.30 70 Label: 225 227 Tick time from program start = 44457.539 Running script 17 at item 226 (-781.45, 606.87) Remaining time to fill dewars is 12600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.07 -0.26 (2nd move error 0.00 -0.02) Finished aligning to item in 1 rounds -3.7 -0.5 unbinned pixels; 2.9 14.0 nm along two shift axes; 2.7 -14.2 nm on specimen axes Total_Shift = 7.417502 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 34.71, -21.85 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.0, 7.0 unbinned pixels, 0.15 nm/sec Measured defocus = -1.27 microns changed by -1.23 to target 34 frames are being saved to U:\krios\20190806\slot11_0491.tif 34 frames are being saved to U:\krios\20190806\slot11_0492.tif 34 frames are being saved to U:\krios\20190806\slot11_0493.tif Item 228: Stage: -781.45 606.87 70 Label: 226 228 Tick time from program start = 44568.986 Running script 17 at item 227 (-780.33, 604.55) Remaining time to fill dewars is 12420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 50 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.15 -0.25 (2nd move error 0.08 -0.01) Finished aligning to item in 1 rounds Trimming A by 20 50 20 20, reference by 20 50 20 20 -2.5 -4.0 unbinned pixels; 11.3 15.1 nm along two shift axes; 16.0 -9.0 nm on specimen axes Total_Shift = 9.409315 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 32.97, -20.37 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -3.7, 11.6 unbinned pixels, 0.22 nm/sec Measured defocus = -1.20 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_0494.tif 34 frames are being saved to U:\krios\20190806\slot11_0495.tif 34 frames are being saved to U:\krios\20190806\slot11_0496.tif Item 229: Stage: -780.33 604.55 70 Label: 227 229 Tick time from program start = 44680.371 Running script 17 at item 228 (-782.61, 603.51) Remaining time to fill dewars is 12360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 70 35 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.19 -0.33 (2nd move error 0.12 -0.09) Finished aligning to item in 1 rounds Trimming A by 20 74 20 20, reference by 20 70 35 20 -10.0 -3.7 unbinned pixels; 0.5 41.6 nm along two shift axes; 16.4 -38.2 nm on specimen axes Total_Shift = 21.340687 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -0.03, 3.72 unbinned pixels, unfiltered CCC is 0.0041 The average drift between shots was -6.7, 4.7 unbinned pixels, 0.15 nm/sec Measured defocus = 0.06 microns changed by -1.46 to target 34 frames are being saved to U:\krios\20190806\slot11_0497.tif 34 frames are being saved to U:\krios\20190806\slot11_0498.tif 34 frames are being saved to U:\krios\20190806\slot11_0499.tif Item 230: Stage: -782.61 603.51 70 Label: 228 230 Tick time from program start = 44792.848 Running script 17 at item 229 (-783.69, 605.83) Remaining time to fill dewars is 12180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.04 -0.30 (2nd move error -0.03 -0.07) Finished aligning to item in 1 rounds -8.6 7.2 unbinned pixels; 37.8 20.2 nm along two shift axes; -26.3 -35.1 nm on specimen axes Total_Shift = 22.499198 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 4.90, -1.96 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -7.4, 0.6 unbinned pixels, 0.13 nm/sec Measured defocus = -0.16 microns changed by -1.34 to target 34 frames are being saved to U:\krios\20190806\slot11_0500.tif 34 frames are being saved to U:\krios\20190806\slot11_0501.tif 34 frames are being saved to U:\krios\20190806\slot11_0502.tif Item 231: Stage: -783.69 605.83 70 Label: 229 231 Tick time from program start = 44904.763 Running script 17 at item 230 (-784.75, 608.23) Remaining time to fill dewars is 12060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.05 -0.33 (2nd move error -0.02 -0.09) Finished aligning to item in 1 rounds -12.5 4.5 unbinned pixels; 32.9 38.3 nm along two shift axes; -14.9 -49.7 nm on specimen axes Total_Shift = 26.621633 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 15.24, -5.96 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -7.8, 0.5 unbinned pixels, 0.14 nm/sec Measured defocus = -0.50 microns changed by -1.10 to target 34 frames are being saved to U:\krios\20190806\slot11_0503.tif 34 frames are being saved to U:\krios\20190806\slot11_0504.tif 34 frames are being saved to U:\krios\20190806\slot11_0505.tif Item 232: Stage: -784.75 608.23 70 Label: 230 232 Tick time from program start = 45016.662 Running script 17 at item 231 (-785.88, 610.66) Remaining time to fill dewars is 11940 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Trimming A by 20 20 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.25 Disparity in stage position after aligning to target position: 0.23 -0.36 (2nd move error 0.10 -0.13) Finished aligning to item in 2 rounds -15.1 -5.6 unbinned pixels; 0.9 62.7 nm along two shift axes; 24.9 -57.5 nm on specimen axes Total_Shift = 32.162912 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 19.66, -9.96 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -4.5, 7.2 unbinned pixels, 0.15 nm/sec Measured defocus = -0.68 microns changed by -1.02 to target 34 frames are being saved to U:\krios\20190806\slot11_0506.tif 34 frames are being saved to U:\krios\20190806\slot11_0507.tif 34 frames are being saved to U:\krios\20190806\slot11_0508.tif Item 233: Stage: -785.88 610.66 70 Label: 231 233 Tick time from program start = 45136.175 Running script 17 at item 232 (-786.99, 612.91) Remaining time to fill dewars is 11760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.06 -0.37 (2nd move error -0.07 -0.14) Finished aligning to item in 1 rounds -21.7 19.3 unbinned pixels; 99.2 49.0 nm along two shift axes; -70.5 -88.4 nm on specimen axes Total_Shift = 58.033795 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 26.07, -14.50 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -4.1, 3.4 unbinned pixels, 0.09 nm/sec Measured defocus = -0.92 microns changed by -0.88 to target 34 frames are being saved to U:\krios\20190806\slot11_0509.tif 34 frames are being saved to U:\krios\20190806\slot11_0510.tif 34 frames are being saved to U:\krios\20190806\slot11_0511.tif Item 234: Stage: -786.99 612.91 70 Label: 232 234 Tick time from program start = 45248.355 Running script 17 at item 233 (-788.11, 615.21) Remaining time to fill dewars is 11700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.38 (2nd move error 0.02 -0.15) Finished aligning to item in 1 rounds -19.2 -2.1 unbinned pixels; 17.3 72.4 nm along two shift axes; 12.3 -74.4 nm on specimen axes Total_Shift = 38.708962 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 31.08, -19.68 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -1.7, 8.9 unbinned pixels, 0.16 nm/sec Measured defocus = -1.14 microns changed by -0.76 to target 34 frames are being saved to U:\krios\20190806\slot11_0512.tif 34 frames are being saved to U:\krios\20190806\slot11_0513.tif 34 frames are being saved to U:\krios\20190806\slot11_0514.tif Item 235: Stage: -788.11 615.21 70 Label: 233 235 Tick time from program start = 45360.192 Running script 17 at item 234 (-789.32, 617.44) Remaining time to fill dewars is 11580 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.35 Disparity in stage position after aligning to target position: 0.21 -0.20 (2nd move error 0.17 0.13) Finished aligning to item in 2 rounds 0.8 -1.8 unbinned pixels; 7.6 0.0 nm along two shift axes; 6.9 3.3 nm on specimen axes Total_Shift = 3.91636 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 37.12, -23.31 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -7.7, 8.6 unbinned pixels, 0.21 nm/sec Measured defocus = -1.35 microns changed by -0.65 to target 34 frames are being saved to U:\krios\20190806\slot11_0515.tif 34 frames are being saved to U:\krios\20190806\slot11_0516.tif 34 frames are being saved to U:\krios\20190806\slot11_0517.tif Item 236: Stage: -789.32 617.44 70 Label: 234 236 Tick time from program start = 45482.793 Running script 17 at item 235 (-790.33, 619.76) Remaining time to fill dewars is 11460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.11 -0.25 (2nd move error 0.07 0.08) Finished aligning to item in 1 rounds 0.1 -3.3 unbinned pixels; 12.2 4.5 nm along two shift axes; 12.7 1.1 nm on specimen axes Total_Shift = 6.551988 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 42.21, -24.67 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -7.1, 9.1 unbinned pixels, 0.21 nm/sec Measured defocus = -1.51 microns changed by -0.59 to target 34 frames are being saved to U:\krios\20190806\slot11_0518.tif 34 frames are being saved to U:\krios\20190806\slot11_0519.tif 34 frames are being saved to U:\krios\20190806\slot11_0520.tif Item 237: Stage: -790.33 619.76 70 Label: 235 237 Tick time from program start = 45597.860 Running script 17 at item 236 (-791.38, 622.09) Remaining time to fill dewars is 11340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.17 -0.28 (2nd move error 0.13 0.05) Finished aligning to item in 1 rounds 0.6 2.9 unbinned pixels; 9.8 6.4 nm along two shift axes; -11.3 1.6 nm on specimen axes Total_Shift = 5.854546 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 46.95, -29.35 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -8.9, 6.5 unbinned pixels, 0.20 nm/sec Measured defocus = -1.71 microns changed by -0.49 to target 34 frames are being saved to U:\krios\20190806\slot11_0521.tif 34 frames are being saved to U:\krios\20190806\slot11_0522.tif 34 frames are being saved to U:\krios\20190806\slot11_0523.tif Item 238: Stage: -791.38 622.09 70 Label: 236 238 Tick time from program start = 45710.024 Running script 17 at item 237 (-792.68, 624.45) Remaining time to fill dewars is 11100 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.10 -0.32 Disparity in stage position after aligning to target position: 0.32 -0.26 (2nd move error 0.21 0.04) Finished aligning to item in 2 rounds -1.4 -5.9 unbinned pixels; 19.8 13.8 nm along two shift axes; 23.1 -4.1 nm on specimen axes Total_Shift = 12.064236 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 46.96, -30.68 unbinned pixels, unfiltered CCC is -0.0008 The average drift between shots was -6.7, 12.1 unbinned pixels, 0.25 nm/sec Measured defocus = -1.73 microns changed by -0.57 to target 34 frames are being saved to U:\krios\20190806\slot11_0524.tif 34 frames are being saved to U:\krios\20190806\slot11_0525.tif 34 frames are being saved to U:\krios\20190806\slot11_0526.tif Item 239: Stage: -792.68 624.45 70 Label: 237 239 Tick time from program start = 45831.346 Running script 17 at item 238 (-793.78, 626.76) Remaining time to fill dewars is 10980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.31 (2nd move error 0.13 -0.01) Finished aligning to item in 1 rounds -1.5 0.9 unbinned pixels; 5.3 4.2 nm along two shift axes; -3.2 -6.1 nm on specimen axes Total_Shift = 3.553614 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 48.84, -32.67 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -3.9, 8.9 unbinned pixels, 0.17 nm/sec Measured defocus = -1.81 microns changed by -0.59 to target 34 frames are being saved to U:\krios\20190806\slot11_0527.tif 34 frames are being saved to U:\krios\20190806\slot11_0528.tif 34 frames are being saved to U:\krios\20190806\slot11_0529.tif Item 240: Stage: -793.78 626.76 70 Label: 238 240 Tick time from program start = 45942.996 Running script 17 at item 239 (-794.81, 629.10) Remaining time to fill dewars is 10860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.35 (2nd move error 0.12 -0.05) Finished aligning to item in 1 rounds -5.6 -1.2 unbinned pixels; 2.8 22.1 nm along two shift axes; 6.0 -21.6 nm on specimen axes Total_Shift = 11.496651 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 48.63, -34.35 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was 0.6, 12.4 unbinned pixels, 0.22 nm/sec Measured defocus = -1.84 microns changed by -0.66 to target 34 frames are being saved to U:\krios\20190806\slot11_0530.tif 34 frames are being saved to U:\krios\20190806\slot11_0531.tif 34 frames are being saved to U:\krios\20190806\slot11_0532.tif Item 241: Stage: -794.81 629.10 70 Label: 239 241 Tick time from program start = 46059.888 Running script 17 at item 240 (-795.89, 631.42) Remaining time to fill dewars is 10800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.18 -0.35 (2nd move error 0.08 -0.05) Finished aligning to item in 1 rounds -4.9 -3.6 unbinned pixels; 7.0 23.0 nm along two shift axes; 15.2 -18.2 nm on specimen axes Total_Shift = 12.167599 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 7.47, -10.61 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was 2.2, 12.5 unbinned pixels, 0.23 nm/sec Measured defocus = -0.37 microns changed by -0.93 to target 34 frames are being saved to U:\krios\20190806\slot11_0533.tif 34 frames are being saved to U:\krios\20190806\slot11_0534.tif 34 frames are being saved to U:\krios\20190806\slot11_0535.tif Item 242: Stage: -795.89 631.42 70 Label: 240 242 Tick time from program start = 46174.673 Running script 17 at item 241 (-796.95, 633.73) Remaining time to fill dewars is 10680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.09 -0.37 (2nd move error -0.01 -0.07) Finished aligning to item in 1 rounds -10.6 4.1 unbinned pixels; 28.9 32.2 nm along two shift axes; -13.7 -42.3 nm on specimen axes Total_Shift = 22.796034 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 5.95, -3.93 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -2.0, 5.5 unbinned pixels, 0.10 nm/sec Measured defocus = -0.22 microns changed by -1.08 to target 34 frames are being saved to U:\krios\20190806\slot11_0536.tif 34 frames are being saved to U:\krios\20190806\slot11_0537.tif 34 frames are being saved to U:\krios\20190806\slot11_0538.tif Item 243: Stage: -796.95 633.73 70 Label: 241 243 Tick time from program start = 46287.025 Running script 17 at item 242 (-797.99, 636.15) Remaining time to fill dewars is 10500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.04 -0.35 (2nd move error -0.07 -0.05) Finished aligning to item in 1 rounds -6.7 13.6 unbinned pixels; 58.6 3.7 nm along two shift axes; -51.3 -28.9 nm on specimen axes Total_Shift = 30.238878 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -0.31, -2.46 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -3.9, 3.1 unbinned pixels, 0.09 nm/sec Measured defocus = -0.03 microns changed by -1.37 to target 34 frames are being saved to U:\krios\20190806\slot11_0539.tif 34 frames are being saved to U:\krios\20190806\slot11_0540.tif 34 frames are being saved to U:\krios\20190806\slot11_0541.tif Item 244: Stage: -797.99 636.15 70 Label: 242 244 Tick time from program start = 46399.128 Running script 17 at item 243 (-799.26, 638.49) Remaining time to fill dewars is 10440 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.14 -0.31 Disparity in stage position after aligning to target position: 0.25 -0.41 (2nd move error 0.10 -0.12) Finished aligning to item in 2 rounds -14.1 -2.3 unbinned pixels; 10.0 54.2 nm along two shift axes; 11.8 -54.4 nm on specimen axes Total_Shift = 28.55709 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is -211.87, 35.72 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was 213.9, -30.0 unbinned pixels, 3.85 nm/sec Measured defocus = 6.17 microns changed by -7.67 to target Tilt-induced shift is 214.27, -133.73 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was 2.5, 4.8 unbinned pixels, 0.10 nm/sec Measured defocus = -7.83 microns changed by 6.33 to target Tilt-induced shift is 129.69, -80.79 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was 0.6, 3.2 unbinned pixels, 0.06 nm/sec Measured defocus = -4.73 microns changed by 3.23 to target Tilt-induced shift is 48.99, -31.00 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was 0.6, 2.4 unbinned pixels, 0.04 nm/sec Measured defocus = -1.79 microns changed by 0.29 to target 34 frames are being saved to U:\krios\20190806\slot11_0542.tif 34 frames are being saved to U:\krios\20190806\slot11_0543.tif 34 frames are being saved to U:\krios\20190806\slot11_0544.tif Item 245: Stage: -799.26 638.49 70 Label: 243 245 Tick time from program start = 46546.346 Running script 17 at item 244 (-800.35, 640.75) Remaining time to fill dewars is 10260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.40 (2nd move error 0.05 -0.10) Finished aligning to item in 1 rounds -13.2 -5.3 unbinned pixels; 2.4 55.5 nm along two shift axes; 23.6 -50.1 nm on specimen axes Total_Shift = 28.444769 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 5.27, -3.24 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -4.4, 9.1 unbinned pixels, 0.18 nm/sec Measured defocus = -0.19 microns changed by -1.41 to target 34 frames are being saved to U:\krios\20190806\slot11_0545.tif 34 frames are being saved to U:\krios\20190806\slot11_0546.tif 34 frames are being saved to U:\krios\20190806\slot11_0547.tif Item 246: Stage: -800.35 640.75 70 Label: 244 246 Tick time from program start = 46658.183 Running script 17 at item 245 (-801.36, 643.10) Remaining time to fill dewars is 10140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.10 -0.42 (2nd move error -0.05 -0.13) Finished aligning to item in 1 rounds -16.7 9.2 unbinned pixels; 55.6 46.1 nm along two shift axes; -32.3 -66.7 nm on specimen axes Total_Shift = 38.058868 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 12.55, -6.26 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -6.6, 3.3 unbinned pixels, 0.13 nm/sec Measured defocus = -0.43 microns changed by -1.27 to target 34 frames are being saved to U:\krios\20190806\slot11_0548.tif 34 frames are being saved to U:\krios\20190806\slot11_0549.tif 34 frames are being saved to U:\krios\20190806\slot11_0550.tif Item 247: Stage: -801.36 643.10 70 Label: 245 247 Tick time from program start = 46770.114 Running script 17 at item 246 (-802.49, 645.47) Remaining time to fill dewars is 9960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.16 -0.41 (2nd move error 0.01 -0.12) Finished aligning to item in 1 rounds -15.7 -0.2 unbinned pixels; 19.6 56.8 nm along two shift axes; 4.2 -60.9 nm on specimen axes Total_Shift = 31.361436 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 16.43, -9.72 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -3.7, 5.1 unbinned pixels, 0.11 nm/sec Measured defocus = -0.59 microns changed by -1.21 to target 34 frames are being saved to U:\krios\20190806\slot11_0551.tif 34 frames are being saved to U:\krios\20190806\slot11_0552.tif 34 frames are being saved to U:\krios\20190806\slot11_0553.tif Item 248: Stage: -802.49 645.47 70 Label: 246 248 Tick time from program start = 46882.310 Running script 17 at item 247 (-803.67, 647.60) Remaining time to fill dewars is 9840 Dewars are NOT busy filling Trimming A by 45 21 20 50, reference by 30 20 20 40 Trimming A by 45 21 20 50, reference by 20 20 20 20 Trimming A by 45 21 20 50, reference by 20 20 20 20 Trimming A by 45 21 20 50, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.31 Trimming A by 70 20 20 35, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.17 -0.16 (2nd move error 0.07 0.13) Finished aligning to item in 2 rounds Trimming A by 70 20 20 50, reference by 70 20 20 35 0.6 -0.9 unbinned pixels; 3.9 0.8 nm along two shift axes; 3.3 2.4 nm on specimen axes Total_Shift = 2.079599 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 17.84, -8.86 unbinned pixels, unfiltered CCC is 0.0049 The average drift between shots was -10.3, 7.8 unbinned pixels, 0.23 nm/sec Measured defocus = -0.61 microns changed by -1.29 to target 34 frames are being saved to U:\krios\20190806\slot11_0554.tif 34 frames are being saved to U:\krios\20190806\slot11_0555.tif 34 frames are being saved to U:\krios\20190806\slot11_0556.tif Item 249: Stage: -803.67 647.60 70 Label: 247 249 Tick time from program start = 47001.635 Running script 17 at item 248 (-806.96, 648.88) Remaining time to fill dewars is 9720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 20 20 90, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.06 -0.26 (2nd move error -0.04 0.03) Finished aligning to item in 1 rounds Trimming A by 135 21 70 80, reference by 130 20 20 90 0.6 8.4 unbinned pixels; 30.1 15.0 nm along two shift axes; -32.9 0.7 nm on specimen axes Total_Shift = 16.872962 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 16.82, -8.99 unbinned pixels, unfiltered CCC is 0.0045 The average drift between shots was -6.5, 3.1 unbinned pixels, 0.13 nm/sec Measured defocus = -0.59 microns changed by -1.41 to target 34 frames are being saved to U:\krios\20190806\slot11_0557.tif 34 frames are being saved to U:\krios\20190806\slot11_0558.tif 34 frames are being saved to U:\krios\20190806\slot11_0559.tif Item 250: Stage: -806.96 648.88 70 Label: 248 250 Tick time from program start = 47114.299 Running script 17 at item 249 (-805.91, 646.53) Remaining time to fill dewars is 9600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 60 20 20 50, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.28 -0.29 (2nd move error 0.18 0.00) Finished aligning to item in 1 rounds Trimming A by 60 21 35 45, reference by 60 20 20 50 0.6 -1.2 unbinned pixels; 5.2 0.5 nm along two shift axes; 4.5 2.7 nm on specimen axes Total_Shift = 2.68075 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 58.03, -38.32 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -3.4, 9.7 unbinned pixels, 0.18 nm/sec Measured defocus = -2.15 microns changed by 0.05 to target 34 frames are being saved to U:\krios\20190806\slot11_0560.tif 34 frames are being saved to U:\krios\20190806\slot11_0561.tif 34 frames are being saved to U:\krios\20190806\slot11_0562.tif Item 251: Stage: -805.91 646.53 70 Label: 249 251 Tick time from program start = 47229.100 Running script 17 at item 250 (-804.86, 644.17) Remaining time to fill dewars is 9480 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.34 -0.26 (2nd move error 0.24 0.03) Finished aligning to item in 1 rounds 2.6 7.7 unbinned pixels; 24.9 21.0 nm along two shift axes; -30.5 8.6 nm on specimen axes Total_Shift = 16.257055 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 27.75, -16.64 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -11.6, 6.9 unbinned pixels, 0.24 nm/sec Measured defocus = -1.00 microns changed by -1.20 to target 34 frames are being saved to U:\krios\20190806\slot11_0563.tif 34 frames are being saved to U:\krios\20190806\slot11_0564.tif 34 frames are being saved to U:\krios\20190806\slot11_0565.tif Item 252: Stage: -804.86 644.17 70 Label: 250 252 Tick time from program start = 47341.717 Running script 17 at item 251 (-803.80, 641.84) Remaining time to fill dewars is 9360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.30 -0.23 (2nd move error 0.21 0.06) Finished aligning to item in 1 rounds -0.9 0.5 unbinned pixels; 3.1 2.3 nm along two shift axes; -1.9 -3.5 nm on specimen axes Total_Shift = 2.019161 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 63.13, -40.29 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -6.7, 11.8 unbinned pixels, 0.24 nm/sec Measured defocus = -2.31 microns changed by 0.01 to target 34 frames are being saved to U:\krios\20190806\slot11_0566.tif 34 frames are being saved to U:\krios\20190806\slot11_0567.tif 34 frames are being saved to U:\krios\20190806\slot11_0568.tif Item 253: Stage: -803.80 641.84 70 Label: 251 253 Tick time from program start = 47454.022 Running script 17 at item 252 (-802.81, 639.54) Remaining time to fill dewars is 9180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.42 -0.19 (2nd move error 0.32 0.10) Finished aligning to item in 1 rounds 0.7 -7.7 unbinned pixels; 29.4 9.1 nm along two shift axes; 30.0 4.4 nm on specimen axes Total_Shift = 15.551925 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 26.67, -16.64 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -8.7, 11.7 unbinned pixels, 0.26 nm/sec Measured defocus = -0.97 microns changed by -1.43 to target 34 frames are being saved to U:\krios\20190806\slot11_0569.tif 34 frames are being saved to U:\krios\20190806\slot11_0570.tif 34 frames are being saved to U:\krios\20190806\slot11_0571.tif Item 254: Stage: -802.81 639.54 70 Label: 252 254 Tick time from program start = 47566.202 Running script 17 at item 253 (-801.44, 637.50) Remaining time to fill dewars is 9120 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.09 -0.26 Disparity in stage position after aligning to target position: 0.19 -0.42 (2nd move error 0.10 -0.18) Finished aligning to item in 2 rounds -17.6 1.8 unbinned pixels; 29.4 60.6 nm along two shift axes; -3.1 -68.7 nm on specimen axes Total_Shift = 35.306064 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 63.24, -41.85 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was 3.7, 11.0 unbinned pixels, 0.20 nm/sec Measured defocus = -2.34 microns changed by -0.16 to target 34 frames are being saved to U:\krios\20190806\slot11_0572.tif 34 frames are being saved to U:\krios\20190806\slot11_0573.tif 34 frames are being saved to U:\krios\20190806\slot11_0574.tif Item 255: Stage: -801.44 637.50 70 Label: 253 255 Tick time from program start = 47685.683 Running script 17 at item 254 (-800.24, 635.02) Remaining time to fill dewars is 9120 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.13 -0.27 Disparity in stage position after aligning to target position: 0.05 -0.36 (2nd move error -0.09 -0.12) Finished aligning to item in 2 rounds -17.9 20.4 unbinned pixels; 98.5 33.7 nm along two shift axes; -75.7 -73.9 nm on specimen axes Total_Shift = 54.321728 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -0.93, 3.37 unbinned pixels, unfiltered CCC is -0.0111 The average drift between shots was -3.3, 1.3 unbinned pixels, 0.06 nm/sec Measured defocus = 0.08 microns changed by -1.38 to target 34 frames are being saved to U:\krios\20190806\slot11_0575.tif 34 frames are being saved to U:\krios\20190806\slot11_0576.tif 34 frames are being saved to U:\krios\20190806\slot11_0577.tif Item 256: Stage: -800.24 635.02 70 Label: 254 256 Tick time from program start = 47807.068 Running script 17 at item 255 (-799.22, 632.65) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.36 (2nd move error 0.01 -0.12) Finished aligning to item in 1 rounds -15.6 -0.1 unbinned pixels; 19.9 56.2 nm along two shift axes; 3.7 -60.5 nm on specimen axes Total_Shift = 31.136999 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 5.79, -2.79 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -1.6, 6.6 unbinned pixels, 0.12 nm/sec Measured defocus = -0.20 microns changed by -1.20 to target 34 frames are being saved to U:\krios\20190806\slot11_0578.tif 34 frames are being saved to U:\krios\20190806\slot11_0579.tif 34 frames are being saved to U:\krios\20190806\slot11_0580.tif Item 257: Stage: -799.22 632.65 70 Label: 255 257 Tick time from program start = 47918.546 Running script 17 at item 256 (-798.07, 630.33) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.14 -0.36 (2nd move error 0.01 -0.11) Finished aligning to item in 1 rounds -14.7 0.4 unbinned pixels; 20.6 52.4 nm along two shift axes; 1.6 -57.4 nm on specimen axes Total_Shift = 29.459082 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 11.58, -8.36 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 0.6, 7.7 unbinned pixels, 0.14 nm/sec Measured defocus = -0.44 microns changed by -1.06 to target 34 frames are being saved to U:\krios\20190806\slot11_0581.tif 34 frames are being saved to U:\krios\20190806\slot11_0582.tif 34 frames are being saved to U:\krios\20190806\slot11_0583.tif Item 258: Stage: -798.07 630.33 70 Label: 256 258 Tick time from program start = 48030.196 Running script 17 at item 257 (-797.02, 628.05) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.16 -0.33 (2nd move error 0.03 -0.09) Finished aligning to item in 1 rounds -11.4 -0.7 unbinned pixels; 12.5 42.0 nm along two shift axes; 4.9 -44.2 nm on specimen axes Total_Shift = 22.820588 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 21.28, -16.06 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was 2.2, 13.3 unbinned pixels, 0.24 nm/sec Measured defocus = -0.82 microns changed by -0.78 to target 34 frames are being saved to U:\krios\20190806\slot11_0584.tif 34 frames are being saved to U:\krios\20190806\slot11_0585.tif 34 frames are being saved to U:\krios\20190806\slot11_0586.tif Item 259: Stage: -797.02 628.05 70 Label: 257 259 Tick time from program start = 48148.180 Running script 17 at item 258 (-795.97, 625.73) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.27 -0.33 (2nd move error 0.13 -0.09) Finished aligning to item in 1 rounds -8.3 -1.5 unbinned pixels; 5.5 32.0 nm along two shift axes; 7.4 -32.0 nm on specimen axes Total_Shift = 16.835394 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 28.49, -19.15 unbinned pixels, unfiltered CCC is 0.0108 The average drift between shots was -0.6, 9.3 unbinned pixels, 0.17 nm/sec Measured defocus = -1.06 microns changed by -0.64 to target 34 frames are being saved to U:\krios\20190806\slot11_0587.tif 34 frames are being saved to U:\krios\20190806\slot11_0588.tif 34 frames are being saved to U:\krios\20190806\slot11_0589.tif Item 260: Stage: -795.97 625.73 70 Label: 258 260 Tick time from program start = 48263.761 Running script 17 at item 259 (-794.70, 623.37) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.34 Disparity in stage position after aligning to target position: 0.08 -0.26 (2nd move error -0.10 0.06) Finished aligning to item in 2 rounds -6.0 30.1 unbinned pixels; 118.3 23.7 nm along two shift axes; -115.7 -29.6 nm on specimen axes Total_Shift = 61.296327 -3.3 20.5 unbinned pixels; 79.7 18.9 nm along two shift axes; -79.1 -17.2 nm on specimen axes Total_Shift = 41.537722 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 31.90, -18.16 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -6.5, 5.1 unbinned pixels, 0.14 nm/sec Measured defocus = -1.13 microns changed by -0.67 to target 34 frames are being saved to U:\krios\20190806\slot11_0590.tif 34 frames are being saved to U:\krios\20190806\slot11_0591.tif 34 frames are being saved to U:\krios\20190806\slot11_0592.tif Item 261: Stage: -794.70 623.37 70 Label: 259 261 Tick time from program start = 48387.938 Running script 17 at item 260 (-793.60, 621.03) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.04 -0.30 (2nd move error -0.13 0.02) Finished aligning to item in 1 rounds -2.7 29.9 unbinned pixels; 113.3 35.2 nm along two shift axes; -115.6 -16.8 nm on specimen axes Total_Shift = 59.978251 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 35.91, -21.40 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -5.1, 3.1 unbinned pixels, 0.10 nm/sec Measured defocus = -1.29 microns changed by -0.61 to target 34 frames are being saved to U:\krios\20190806\slot11_0593.tif 34 frames are being saved to U:\krios\20190806\slot11_0594.tif 34 frames are being saved to U:\krios\20190806\slot11_0595.tif Item 262: Stage: -793.60 621.03 70 Label: 260 262 Tick time from program start = 48499.790 Running script 17 at item 261 (-792.57, 618.69) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.27 (2nd move error 0.01 0.04) Finished aligning to item in 1 rounds -1.2 -2.0 unbinned pixels; 5.7 7.1 nm along two shift axes; 7.9 -4.1 nm on specimen axes Total_Shift = 4.569521 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 36.47, -23.44 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -10.9, 8.9 unbinned pixels, 0.25 nm/sec Measured defocus = -1.34 microns changed by -0.66 to target 34 frames are being saved to U:\krios\20190806\slot11_0596.tif 34 frames are being saved to U:\krios\20190806\slot11_0597.tif 34 frames are being saved to U:\krios\20190806\slot11_0598.tif Item 263: Stage: -792.57 618.69 70 Label: 261 263 Tick time from program start = 48614.248 Running script 17 at item 262 (-791.46, 616.34) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.28 (2nd move error 0.05 0.04) Finished aligning to item in 1 rounds 0.3 1.8 unbinned pixels; 6.2 3.9 nm along two shift axes; -7.0 0.9 nm on specimen axes Total_Shift = 3.649623 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 38.44, -26.43 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -10.6, 12.3 unbinned pixels, 0.29 nm/sec Measured defocus = -1.44 microns changed by -0.66 to target 34 frames are being saved to U:\krios\20190806\slot11_0599.tif 34 frames are being saved to U:\krios\20190806\slot11_0600.tif 34 frames are being saved to U:\krios\20190806\slot11_0601.tif Item 264: Stage: -791.46 616.34 70 Label: 262 264 Tick time from program start = 48725.711 Running script 17 at item 263 (-790.46, 614.04) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.26 (2nd move error 0.01 0.06) Finished aligning to item in 1 rounds -1.3 -2.0 unbinned pixels; 5.6 7.6 nm along two shift axes; 7.9 -4.6 nm on specimen axes Total_Shift = 4.699313 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 39.16, -26.15 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -8.6, 11.2 unbinned pixels, 0.25 nm/sec Measured defocus = -1.45 microns changed by -0.75 to target 34 frames are being saved to U:\krios\20190806\slot11_0602.tif 34 frames are being saved to U:\krios\20190806\slot11_0603.tif 34 frames are being saved to U:\krios\20190806\slot11_0604.tif Item 265: Stage: -790.46 614.04 70 Label: 263 265 Tick time from program start = 48838.546 Running script 17 at item 264 (-789.43, 611.77) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.31 -0.26 (2nd move error 0.13 0.06) Finished aligning to item in 1 rounds -2.1 -1.1 unbinned pixels; 1.3 9.4 nm along two shift axes; 4.8 -8.1 nm on specimen axes Total_Shift = 4.835618 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 40.80, -24.41 unbinned pixels, unfiltered CCC is -0.0008 The average drift between shots was -12.0, 11.1 unbinned pixels, 0.29 nm/sec Measured defocus = -1.47 microns changed by -0.83 to target 34 frames are being saved to U:\krios\20190806\slot11_0605.tif 34 frames are being saved to U:\krios\20190806\slot11_0606.tif 34 frames are being saved to U:\krios\20190806\slot11_0607.tif Item 266: Stage: -789.43 611.77 70 Label: 264 266 Tick time from program start = 48951.959 Running script 17 at item 265 (-788.11, 609.37) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 20 45 20 20, reference by 20 20 20 20 Trimming A by 20 45 20 20, reference by 20 20 20 20 Trimming A by 20 45 20 20, reference by 20 20 20 20 Trimming A by 20 45 20 20, reference by 24 100 20 90 Trimming A by 20 45 20 20, reference by 20 50 20 20 Trimming A by 20 45 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: 0.05 -0.32 Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.14 -0.13 (2nd move error 0.08 0.17) Finished aligning to item in 2 rounds 0.3 4.7 unbinned pixels; 16.9 8.1 nm along two shift axes; -18.3 0.1 nm on specimen axes Total_Shift = 9.417211 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 39.58, -23.67 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -10.8, 7.1 unbinned pixels, 0.23 nm/sec Measured defocus = -1.43 microns changed by -0.97 to target 34 frames are being saved to U:\krios\20190806\slot11_0608.tif 34 frames are being saved to U:\krios\20190806\slot11_0609.tif 34 frames are being saved to U:\krios\20190806\slot11_0610.tif Item 267: Stage: -788.11 609.37 70 Label: 265 267 Tick time from program start = 49072.064 Running script 17 at item 266 (-786.97, 607.13) Remaining time to fill dewars is 8880 Dewars are NOT busy filling Trimming A by 20 60 20 20, reference by 20 20 20 20 Trimming A by 20 60 20 20, reference by 20 20 20 20 Trimming A by 20 60 20 20, reference by 20 20 20 20 Trimming A by 20 60 20 20, reference by 20 50 20 20 Trimming A by 20 60 20 20, reference by 20 60 20 20 Trimming A by 20 60 20 20, reference by 20 100 65 20 Disparity in stage position after aligning to center of map frame: -0.02 -0.23 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: -0.12 -0.31 (2nd move error -0.11 -0.10) Finished aligning to item in 2 rounds -13.2 21.1 unbinned pixels; 94.9 15.5 nm along two shift axes; -79.5 -55.6 nm on specimen axes Total_Shift = 49.810706 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 73.50, -47.60 unbinned pixels, unfiltered CCC is -0.0008 The average drift between shots was -4.1, 3.8 unbinned pixels, 0.10 nm/sec Measured defocus = -2.71 microns changed by 0.21 to target 34 frames are being saved to U:\krios\20190806\slot11_0611.tif 34 frames are being saved to U:\krios\20190806\slot11_0612.tif 34 frames are being saved to U:\krios\20190806\slot11_0613.tif Item 268: Stage: -786.97 607.13 70 Label: 266 268 Tick time from program start = 49196.428 Running script 17 at item 267 (-785.91, 604.71) Remaining time to fill dewars is 7800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 30 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: 0.10 -0.31 (2nd move error 0.11 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 30 20 20, reference by 20 30 20 20 -12.7 -4.9 unbinned pixels; 1.3 53.0 nm along two shift axes; 21.6 -48.3 nm on specimen axes Total_Shift = 27.162208 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 4.48, 0.86 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -7.5, 7.8 unbinned pixels, 0.19 nm/sec Measured defocus = -0.10 microns changed by -1.20 to target 34 frames are being saved to U:\krios\20190806\slot11_0614.tif 34 frames are being saved to U:\krios\20190806\slot11_0615.tif 34 frames are being saved to U:\krios\20190806\slot11_0616.tif Item 269: Stage: -785.91 604.71 70 Label: 267 269 Tick time from program start = 49309.014 Running script 17 at item 268 (-788.17, 603.57) Remaining time to fill dewars is 7680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 60 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.23 -0.25 (2nd move error 0.19 0.05) Finished aligning to item in 1 rounds Trimming A by 20 60 20 20, reference by 20 60 20 20 0.7 2.5 unbinned pixels; 8.5 6.3 nm along two shift axes; -10.0 2.1 nm on specimen axes Total_Shift = 5.259933 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -1.26, 1.55 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -9.2, 3.8 unbinned pixels, 0.18 nm/sec Measured defocus = 0.06 microns changed by -1.36 to target 34 frames are being saved to U:\krios\20190806\slot11_0617.tif 34 frames are being saved to U:\krios\20190806\slot11_0618.tif 34 frames are being saved to U:\krios\20190806\slot11_0619.tif Item 270: Stage: -788.17 603.57 70 Label: 268 270 Tick time from program start = 49421.897 Running script 17 at item 269 (-789.27, 605.94) Remaining time to fill dewars is 7560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.17 -0.22 (2nd move error 0.11 0.08) Finished aligning to item in 1 rounds 1.1 -2.9 unbinned pixels; 12.3 0.5 nm along two shift axes; 11.2 4.9 nm on specimen axes Total_Shift = 6.283738 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 2.81, -1.24 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -9.4, 6.7 unbinned pixels, 0.21 nm/sec Measured defocus = -0.09 microns changed by -1.31 to target 34 frames are being saved to U:\krios\20190806\slot11_0620.tif 34 frames are being saved to U:\krios\20190806\slot11_0621.tif 34 frames are being saved to U:\krios\20190806\slot11_0622.tif Item 271: Stage: -789.27 605.94 70 Label: 269 271 Tick time from program start = 49534.420 Running script 17 at item 270 (-790.35, 608.23) Remaining time to fill dewars is 7380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.08 -0.23 (2nd move error 0.02 0.07) Finished aligning to item in 1 rounds 0.5 -2.8 unbinned pixels; 11.0 2.3 nm along two shift axes; 10.8 2.7 nm on specimen axes Total_Shift = 5.723561 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 9.72, -4.94 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -9.2, 8.5 unbinned pixels, 0.22 nm/sec Measured defocus = -0.34 microns changed by -1.16 to target 34 frames are being saved to U:\krios\20190806\slot11_0623.tif 34 frames are being saved to U:\krios\20190806\slot11_0624.tif 34 frames are being saved to U:\krios\20190806\slot11_0625.tif Item 272: Stage: -790.35 608.23 70 Label: 270 272 Tick time from program start = 49645.914 Running script 17 at item 271 (-791.58, 610.65) Remaining time to fill dewars is 7320 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: 0.07 -0.33 Disparity in stage position after aligning to target position: 0.34 -0.25 (2nd move error 0.27 0.06) Finished aligning to item in 2 rounds -0.4 6.1 unbinned pixels; 23.0 7.7 nm along two shift axes; -23.7 -2.9 nm on specimen axes Total_Shift = 12.245672 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 15.66, -9.00 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -13.9, 9.3 unbinned pixels, 0.30 nm/sec Measured defocus = -0.56 microns changed by -1.04 to target 34 frames are being saved to U:\krios\20190806\slot11_0626.tif 34 frames are being saved to U:\krios\20190806\slot11_0627.tif 34 frames are being saved to U:\krios\20190806\slot11_0628.tif Item 273: Stage: -791.58 610.65 70 Label: 271 273 Tick time from program start = 49767.501 Running script 17 at item 272 (-792.71, 612.99) Remaining time to fill dewars is 7140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.26 -0.31 (2nd move error 0.19 -0.00) Finished aligning to item in 1 rounds -0.9 -6.3 unbinned pixels; 22.0 12.5 nm along two shift axes; 24.6 -2.0 nm on specimen axes Total_Shift = 12.670426 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 16.92, -13.23 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -7.3, 14.2 unbinned pixels, 0.28 nm/sec Measured defocus = -0.66 microns changed by -1.04 to target 34 frames are being saved to U:\krios\20190806\slot11_0629.tif 34 frames are being saved to U:\krios\20190806\slot11_0630.tif 34 frames are being saved to U:\krios\20190806\slot11_0631.tif Item 274: Stage: -792.71 612.99 70 Label: 272 274 Tick time from program start = 49883.831 Running script 17 at item 273 (-793.77, 615.31) Remaining time to fill dewars is 7020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.31 (2nd move error 0.08 -0.00) Finished aligning to item in 1 rounds -1.8 -0.2 unbinned pixels; 1.4 6.8 nm along two shift axes; 1.3 -6.9 nm on specimen axes Total_Shift = 3.590558 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 21.66, -15.37 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -9.6, 11.4 unbinned pixels, 0.27 nm/sec Measured defocus = -0.82 microns changed by -0.98 to target 34 frames are being saved to U:\krios\20190806\slot11_0632.tif 34 frames are being saved to U:\krios\20190806\slot11_0633.tif 34 frames are being saved to U:\krios\20190806\slot11_0634.tif Item 275: Stage: -793.77 615.31 70 Label: 273 275 Tick time from program start = 49995.949 Running script 17 at item 274 (-794.82, 617.59) Remaining time to fill dewars is 6900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.17 -0.32 (2nd move error 0.10 -0.01) Finished aligning to item in 1 rounds -2.8 -1.3 unbinned pixels; 1.1 12.1 nm along two shift axes; 5.7 -10.7 nm on specimen axes Total_Shift = 6.230741 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 30.78, -18.76 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -8.9, 15.3 unbinned pixels, 0.32 nm/sec Measured defocus = -1.11 microns changed by -0.79 to target 34 frames are being saved to U:\krios\20190806\slot11_0635.tif 34 frames are being saved to U:\krios\20190806\slot11_0636.tif 34 frames are being saved to U:\krios\20190806\slot11_0637.tif Item 276: Stage: -794.82 617.59 70 Label: 274 276 Tick time from program start = 50108.239 Running script 17 at item 275 (-795.92, 619.98) Remaining time to fill dewars is 6780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.12 -0.32 (2nd move error 0.05 -0.01) Finished aligning to item in 1 rounds -4.1 -3.6 unbinned pixels; 8.1 20.1 nm along two shift axes; 15.0 -15.1 nm on specimen axes Total_Shift = 10.915857 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 31.03, -21.04 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -5.3, 11.1 unbinned pixels, 0.22 nm/sec Measured defocus = -1.16 microns changed by -0.84 to target 34 frames are being saved to U:\krios\20190806\slot11_0638.tif 34 frames are being saved to U:\krios\20190806\slot11_0639.tif 34 frames are being saved to U:\krios\20190806\slot11_0640.tif Item 277: Stage: -795.92 619.98 70 Label: 275 277 Tick time from program start = 50222.915 Running script 17 at item 276 (-796.98, 622.35) Remaining time to fill dewars is 6660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.02 -0.33 (2nd move error -0.06 -0.03) Finished aligning to item in 1 rounds -4.4 11.6 unbinned pixels; 48.5 1.7 nm along two shift axes; -44.3 -19.5 nm on specimen axes Total_Shift = 24.860858 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 36.14, -23.06 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -5.2, 4.9 unbinned pixels, 0.13 nm/sec Measured defocus = -1.33 microns changed by -0.77 to target 34 frames are being saved to U:\krios\20190806\slot11_0641.tif 34 frames are being saved to U:\krios\20190806\slot11_0642.tif 34 frames are being saved to U:\krios\20190806\slot11_0643.tif Item 278: Stage: -796.98 622.35 70 Label: 276 278 Tick time from program start = 50336.499 Running script 17 at item 277 (-798.24, 624.73) Remaining time to fill dewars is 6600 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.17 -0.28 Disparity in stage position after aligning to target position: 0.19 -0.41 (2nd move error 0.01 -0.15) Finished aligning to item in 2 rounds -16.5 0.1 unbinned pixels; 22.1 59.2 nm along two shift axes; 2.9 -64.2 nm on specimen axes Total_Shift = 33.016151 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 38.14, -27.13 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -0.4, 5.8 unbinned pixels, 0.10 nm/sec Measured defocus = -1.44 microns changed by -0.76 to target 34 frames are being saved to U:\krios\20190806\slot11_0644.tif 34 frames are being saved to U:\krios\20190806\slot11_0645.tif 34 frames are being saved to U:\krios\20190806\slot11_0646.tif Item 279: Stage: -798.24 624.73 70 Label: 277 279 Tick time from program start = 50471.924 Running script 17 at item 278 (-799.24, 626.94) Remaining time to fill dewars is 6360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.21 -0.40 (2nd move error 0.03 -0.14) Finished aligning to item in 1 rounds -16.8 -2.6 unbinned pixels; 12.3 64.2 nm along two shift axes; 13.7 -64.6 nm on specimen axes Total_Shift = 33.910127 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 38.79, -27.50 unbinned pixels, unfiltered CCC is -0.0010 The average drift between shots was 5.2, 12.1 unbinned pixels, 0.23 nm/sec Measured defocus = -1.47 microns changed by -0.83 to target 34 frames are being saved to U:\krios\20190806\slot11_0647.tif 34 frames are being saved to U:\krios\20190806\slot11_0648.tif 34 frames are being saved to U:\krios\20190806\slot11_0649.tif Item 280: Stage: -799.24 626.94 70 Label: 278 280 Tick time from program start = 50584.042 Running script 17 at item 279 (-800.35, 629.31) Remaining time to fill dewars is 6240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.14 -0.42 (2nd move error -0.03 -0.16) Finished aligning to item in 1 rounds -25.1 25.2 unbinned pixels; 125.5 52.4 nm along two shift axes; -92.8 -103.0 nm on specimen axes Total_Shift = 71.170757 -10.6 18.3 unbinned pixels; 81.0 10.6 nm along two shift axes; -68.9 -45.0 nm on specimen axes Total_Shift = 42.248153 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 36.28, -23.36 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was 3.6, 4.7 unbinned pixels, 0.11 nm/sec Measured defocus = -1.33 microns changed by -1.07 to target 34 frames are being saved to U:\krios\20190806\slot11_0650.tif 34 frames are being saved to U:\krios\20190806\slot11_0651.tif 34 frames are being saved to U:\krios\20190806\slot11_0652.tif Item 281: Stage: -800.35 629.31 70 Label: 279 281 Tick time from program start = 50702.228 Running script 17 at item 280 (-801.43, 631.62) Remaining time to fill dewars is 6120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.04 -0.40 (2nd move error -0.13 -0.14) Finished aligning to item in 1 rounds -18.8 24.1 unbinned pixels; 113.1 31.3 nm along two shift axes; -89.8 -78.1 nm on specimen axes Total_Shift = 61.111819 -10.6 15.0 unbinned pixels; 68.9 15.8 nm along two shift axes; -55.9 -44.6 nm on specimen axes Total_Shift = 36.715175 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 68.47, -46.84 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 4.0, 5.5 unbinned pixels, 0.12 nm/sec Measured defocus = -2.56 microns changed by 0.06 to target 34 frames are being saved to U:\krios\20190806\slot11_0653.tif 34 frames are being saved to U:\krios\20190806\slot11_0654.tif 34 frames are being saved to U:\krios\20190806\slot11_0655.tif Item 282: Stage: -801.43 631.62 70 Label: 280 282 Tick time from program start = 50819.915 Running script 17 at item 281 (-802.55, 633.70) Remaining time to fill dewars is 6060 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.05 -0.27 Disparity in stage position after aligning to target position: 0.19 -0.13 (2nd move error 0.14 0.12) Finished aligning to item in 2 rounds 1.4 -4.2 unbinned pixels; 17.2 1.2 nm along two shift axes; 15.9 6.4 nm on specimen axes Total_Shift = 8.811269 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -3.22, 1.92 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -5.5, 8.5 unbinned pixels, 0.18 nm/sec Measured defocus = 0.12 microns changed by -1.42 to target 34 frames are being saved to U:\krios\20190806\slot11_0656.tif 34 frames are being saved to U:\krios\20190806\slot11_0657.tif 34 frames are being saved to U:\krios\20190806\slot11_0658.tif Item 283: Stage: -802.55 633.70 70 Label: 281 283 Tick time from program start = 50939.599 Running script 17 at item 282 (-803.62, 635.99) Remaining time to fill dewars is 5880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.08 -0.18 (2nd move error 0.03 0.07) Finished aligning to item in 1 rounds 0.8 -2.2 unbinned pixels; 9.1 0.5 nm along two shift axes; 8.4 3.6 nm on specimen axes Total_Shift = 4.681173 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -3.18, 3.08 unbinned pixels, unfiltered CCC is 0.0069 The average drift between shots was -6.4, 7.1 unbinned pixels, 0.17 nm/sec Measured defocus = 0.13 microns changed by -1.53 to target Tilt-induced shift is 37.30, -23.90 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was -1.4, 4.1 unbinned pixels, 0.08 nm/sec Measured defocus = -1.37 microns changed by -0.03 to target 34 frames are being saved to U:\krios\20190806\slot11_0659.tif 34 frames are being saved to U:\krios\20190806\slot11_0660.tif 34 frames are being saved to U:\krios\20190806\slot11_0661.tif Item 284: Stage: -803.62 635.99 70 Label: 282 284 Tick time from program start = 51060.718 Running script 17 at item 283 (-805.05, 638.48) Remaining time to fill dewars is 5820 Dewars are NOT busy filling Trimming A by 45 21 20 50, reference by 30 20 20 40 Trimming A by 45 21 20 50, reference by 20 20 20 20 Trimming A by 45 21 20 50, reference by 20 20 20 20 Trimming A by 45 21 20 50, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.31 Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.39 -0.20 (2nd move error 0.29 0.09) Finished aligning to item in 2 rounds 0.6 2.0 unbinned pixels; 6.7 5.2 nm along two shift axes; -8.1 1.9 nm on specimen axes Total_Shift = 4.256812 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is -2.91, -0.46 unbinned pixels, unfiltered CCC is 0.0147 The average drift between shots was -10.3, 7.2 unbinned pixels, 0.22 nm/sec Measured defocus = 0.07 microns changed by -1.57 to target Tilt-induced shift is 37.18, -24.33 unbinned pixels, unfiltered CCC is 0.0130 The average drift between shots was -2.2, 4.6 unbinned pixels, 0.09 nm/sec Measured defocus = -1.37 microns changed by -0.13 to target 34 frames are being saved to U:\krios\20190806\slot11_0662.tif 34 frames are being saved to U:\krios\20190806\slot11_0663.tif 34 frames are being saved to U:\krios\20190806\slot11_0664.tif Item 285: Stage: -805.05 638.48 70 Label: 283 285 Tick time from program start = 51192.227 Running script 17 at item 284 (-806.01, 640.74) Remaining time to fill dewars is 5580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.35 -0.24 (2nd move error 0.25 0.05) Finished aligning to item in 1 rounds -0.4 -4.0 unbinned pixels; 14.3 7.5 nm along two shift axes; 15.7 -0.7 nm on specimen axes Total_Shift = 8.074393 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is -1.69, 4.27 unbinned pixels, unfiltered CCC is 0.0056 The average drift between shots was -2.7, 4.5 unbinned pixels, 0.09 nm/sec Measured defocus = 0.11 microns changed by -1.71 to target Tilt-induced shift is 47.15, -29.54 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -0.9, 5.8 unbinned pixels, 0.10 nm/sec Measured defocus = -1.72 microns changed by 0.12 to target 34 frames are being saved to U:\krios\20190806\slot11_0665.tif 34 frames are being saved to U:\krios\20190806\slot11_0666.tif 34 frames are being saved to U:\krios\20190806\slot11_0667.tif Item 286: Stage: -806.01 640.74 70 Label: 284 286 Tick time from program start = 51314.158 Running script 17 at item 285 (-807.07, 643.08) Remaining time to fill dewars is 5460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.23 -0.28 (2nd move error 0.12 0.01) Finished aligning to item in 1 rounds 2.3 -1.7 unbinned pixels; 9.3 5.6 nm along two shift axes; 6.2 9.2 nm on specimen axes Total_Shift = 5.699552 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 9.64, -5.84 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -9.6, 8.5 unbinned pixels, 0.23 nm/sec Measured defocus = -0.35 microns changed by -1.35 to target 34 frames are being saved to U:\krios\20190806\slot11_0668.tif 34 frames are being saved to U:\krios\20190806\slot11_0669.tif 34 frames are being saved to U:\krios\20190806\slot11_0670.tif Item 287: Stage: -807.07 643.08 70 Label: 285 287 Tick time from program start = 51426.977 Running script 17 at item 286 (-808.17, 645.42) Remaining time to fill dewars is 5340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 60 20 20 60, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.27 -0.29 (2nd move error 0.18 0.01) Finished aligning to item in 1 rounds Trimming A by 55 21 20 60, reference by 60 20 20 60 1.0 -6.0 unbinned pixels; 23.1 5.5 nm along two shift axes; 22.9 5.0 nm on specimen axes Total_Shift = 12.060089 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 13.06, -7.08 unbinned pixels, unfiltered CCC is 0.0055 The average drift between shots was -5.9, 9.8 unbinned pixels, 0.20 nm/sec Measured defocus = -0.46 microns changed by -1.34 to target 34 frames are being saved to U:\krios\20190806\slot11_0671.tif 34 frames are being saved to U:\krios\20190806\slot11_0672.tif 34 frames are being saved to U:\krios\20190806\slot11_0673.tif Item 288: Stage: -808.17 645.42 70 Label: 286 288 Tick time from program start = 51539.423 Running script 17 at item 287 (-809.22, 647.82) Remaining time to fill dewars is 5220 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 23 20 100, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.17 -0.30 (2nd move error 0.07 -0.01) Finished aligning to item in 1 rounds Trimming A by 130 21 60 95, reference by 130 23 20 100 -3.2 -2.9 unbinned pixels; 6.3 15.7 nm along two shift axes; 11.8 -11.8 nm on specimen axes Total_Shift = 8.548302 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 16.52, -11.24 unbinned pixels, unfiltered CCC is 0.0107 The average drift between shots was -3.1, 8.9 unbinned pixels, 0.16 nm/sec Measured defocus = -0.62 microns changed by -1.28 to target 34 frames are being saved to U:\krios\20190806\slot11_0674.tif 34 frames are being saved to U:\krios\20190806\slot11_0675.tif 34 frames are being saved to U:\krios\20190806\slot11_0676.tif Item 289: Stage: -809.22 647.82 70 Label: 287 289 Tick time from program start = 51651.712 Running script 17 at item 288 (-811.45, 646.72) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 130 24 20 130, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.12 -0.35 (2nd move error 0.02 -0.06) Finished aligning to item in 1 rounds Trimming A by 130 24 20 130, reference by 130 24 20 130 -8.8 -2.9 unbinned pixels; 0.9 35.8 nm along two shift axes; 13.0 -33.5 nm on specimen axes Total_Shift = 18.431394 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 16.48, -11.82 unbinned pixels, unfiltered CCC is 0.0033 The average drift between shots was -3.4, 9.5 unbinned pixels, 0.18 nm/sec Measured defocus = -0.63 microns changed by -1.37 to target 34 frames are being saved to U:\krios\20190806\slot11_0677.tif 34 frames are being saved to U:\krios\20190806\slot11_0678.tif 34 frames are being saved to U:\krios\20190806\slot11_0679.tif Item 290: Stage: -811.45 646.72 70 Label: 288 290 Tick time from program start = 51763.924 Running script 17 at item 289 (-810.40, 644.34) Remaining time to fill dewars is 4980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 70 20 20 85, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.22 -0.34 (2nd move error 0.12 -0.05) Finished aligning to item in 1 rounds Trimming A by 65 21 20 85, reference by 70 20 20 85 -4.8 -0.5 unbinned pixels; 4.3 17.9 nm along two shift axes; 3.1 -18.4 nm on specimen axes Total_Shift = 9.568369 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 56.04, -35.67 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -3.0, 11.3 unbinned pixels, 0.21 nm/sec Measured defocus = -2.05 microns changed by -0.05 to target 34 frames are being saved to U:\krios\20190806\slot11_0680.tif 34 frames are being saved to U:\krios\20190806\slot11_0681.tif 34 frames are being saved to U:\krios\20190806\slot11_0682.tif Item 291: Stage: -810.40 644.34 70 Label: 289 291 Tick time from program start = 51876.604 Running script 17 at item 290 (-809.28, 641.99) Remaining time to fill dewars is 4860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 25 20 20 30, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.30 -0.34 (2nd move error 0.19 -0.05) Finished aligning to item in 1 rounds Trimming A by 20 20 20 30, reference by 25 20 20 30 -4.9 -1.3 unbinned pixels; 1.5 19.6 nm along two shift axes; 6.2 -18.7 nm on specimen axes Total_Shift = 10.116599 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 19.98, -12.04 unbinned pixels, unfiltered CCC is 0.0035 The average drift between shots was -6.1, 9.5 unbinned pixels, 0.20 nm/sec Measured defocus = -0.72 microns changed by -1.48 to target 34 frames are being saved to U:\krios\20190806\slot11_0683.tif 34 frames are being saved to U:\krios\20190806\slot11_0684.tif 34 frames are being saved to U:\krios\20190806\slot11_0685.tif Item 292: Stage: -809.28 641.99 70 Label: 290 292 Tick time from program start = 51989.018 Running script 17 at item 291 (-808.24, 639.68) Remaining time to fill dewars is 4740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.43 -0.30 (2nd move error 0.33 -0.00) Finished aligning to item in 1 rounds 2.6 -1.1 unbinned pixels; 7.6 7.8 nm along two shift axes; 3.8 10.5 nm on specimen axes Total_Shift = 5.720634 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 53.48, -34.99 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -4.8, 9.1 unbinned pixels, 0.18 nm/sec Measured defocus = -1.97 microns changed by -0.33 to target 34 frames are being saved to U:\krios\20190806\slot11_0686.tif 34 frames are being saved to U:\krios\20190806\slot11_0687.tif 34 frames are being saved to U:\krios\20190806\slot11_0688.tif Item 293: Stage: -808.24 639.68 70 Label: 291 293 Tick time from program start = 52101.120 Running script 17 at item 292 (-807.26, 637.42) Remaining time to fill dewars is 4620 Dewars are NOT busy filling Trimming A by 45 21 20 55, reference by 30 20 20 40 Trimming A by 45 21 20 55, reference by 20 20 20 20 Trimming A by 45 21 20 55, reference by 20 20 20 20 Trimming A by 45 21 20 55, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: 0.25 -0.33 Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.34 -0.25 (2nd move error 0.07 0.07) Finished aligning to item in 2 rounds -1.5 0.9 unbinned pixels; 5.2 4.0 nm along two shift axes; -3.2 -6.0 nm on specimen axes Total_Shift = 3.464368 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 22.26, -12.70 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was -4.5, 6.9 unbinned pixels, 0.15 nm/sec Measured defocus = -0.79 microns changed by -1.61 to target Tilt-induced shift is 63.11, -39.72 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -1.2, 3.9 unbinned pixels, 0.07 nm/sec Measured defocus = -2.30 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_0689.tif 34 frames are being saved to U:\krios\20190806\slot11_0690.tif 34 frames are being saved to U:\krios\20190806\slot11_0691.tif Item 294: Stage: -807.26 637.42 70 Label: 292 294 Tick time from program start = 52235.390 Running script 17 at item 293 (-805.85, 634.97) Remaining time to fill dewars is 4440 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.24 -0.31 Disparity in stage position after aligning to target position: 0.20 -0.16 (2nd move error -0.05 0.13) Finished aligning to item in 2 rounds 1.8 9.6 unbinned pixels; 32.9 20.9 nm along two shift axes; -37.7 4.9 nm on specimen axes Total_Shift = 19.505187 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 62.16, -42.76 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -2.2, 6.4 unbinned pixels, 0.12 nm/sec Measured defocus = -2.33 microns changed by -0.17 to target 34 frames are being saved to U:\krios\20190806\slot11_0692.tif 34 frames are being saved to U:\krios\20190806\slot11_0693.tif 34 frames are being saved to U:\krios\20190806\slot11_0694.tif Item 295: Stage: -805.85 634.97 70 Label: 293 295 Tick time from program start = 52354.091 Running script 17 at item 294 (-804.80, 632.63) Remaining time to fill dewars is 4380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.22 -0.17 (2nd move error -0.03 0.12) Finished aligning to item in 1 rounds 1.5 5.9 unbinned pixels; 19.9 14.3 nm along two shift axes; -23.4 4.6 nm on specimen axes Total_Shift = 12.237724 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -1.65, 0.61 unbinned pixels, unfiltered CCC is -0.0013 The average drift between shots was -6.7, 1.5 unbinned pixels, 0.12 nm/sec Measured defocus = 0.05 microns changed by -1.35 to target 34 frames are being saved to U:\krios\20190806\slot11_0695.tif 34 frames are being saved to U:\krios\20190806\slot11_0696.tif 34 frames are being saved to U:\krios\20190806\slot11_0697.tif Item 296: Stage: -804.80 632.63 70 Label: 294 296 Tick time from program start = 52465.788 Running script 17 at item 295 (-803.78, 630.31) Remaining time to fill dewars is 4200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.16 (2nd move error -0.05 0.13) Finished aligning to item in 1 rounds 3.0 10.9 unbinned pixels; 36.1 27.1 nm along two shift axes; -42.9 9.4 nm on specimen axes Total_Shift = 22.551951 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 7.03, -3.00 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -9.2, 3.0 unbinned pixels, 0.17 nm/sec Measured defocus = -0.23 microns changed by -1.07 to target 34 frames are being saved to U:\krios\20190806\slot11_0698.tif 34 frames are being saved to U:\krios\20190806\slot11_0699.tif 34 frames are being saved to U:\krios\20190806\slot11_0700.tif Item 297: Stage: -803.78 630.31 70 Label: 295 297 Tick time from program start = 52577.766 Running script 17 at item 296 (-802.73, 628.01) Remaining time to fill dewars is 4200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.32 -0.15 (2nd move error 0.08 0.14) Finished aligning to item in 1 rounds 1.3 -1.3 unbinned pixels; 6.3 2.9 nm along two shift axes; 4.6 5.4 nm on specimen axes Total_Shift = 3.649627 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 9.70, -5.62 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -7.8, 8.2 unbinned pixels, 0.20 nm/sec Measured defocus = -0.35 microns changed by -1.05 to target 34 frames are being saved to U:\krios\20190806\slot11_0701.tif 34 frames are being saved to U:\krios\20190806\slot11_0702.tif 34 frames are being saved to U:\krios\20190806\slot11_0703.tif Item 298: Stage: -802.73 628.01 70 Label: 296 298 Tick time from program start = 52693.004 Running script 17 at item 297 (-801.74, 625.65) Remaining time to fill dewars is 4080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.33 -0.13 (2nd move error 0.09 0.15) Finished aligning to item in 1 rounds 0.7 6.1 unbinned pixels; 21.4 11.7 nm along two shift axes; -23.8 1.5 nm on specimen axes Total_Shift = 12.233752 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 17.15, -9.21 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -10.8, 5.1 unbinned pixels, 0.21 nm/sec Measured defocus = -0.60 microns changed by -0.90 to target 34 frames are being saved to U:\krios\20190806\slot11_0704.tif 34 frames are being saved to U:\krios\20190806\slot11_0705.tif 34 frames are being saved to U:\krios\20190806\slot11_0706.tif Item 299: Stage: -801.74 625.65 70 Label: 297 299 Tick time from program start = 52808.398 Running script 17 at item 298 (-800.52, 623.64) Remaining time to fill dewars is 3900 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.09 -0.23 Disparity in stage position after aligning to target position: 0.13 -0.39 (2nd move error 0.04 -0.19) Finished aligning to item in 2 rounds -22.5 -4.1 unbinned pixels; 14.4 87.0 nm along two shift axes; 20.5 -86.6 nm on specimen axes Total_Shift = 45.667593 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 20.82, -12.54 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -2.0, 8.6 unbinned pixels, 0.16 nm/sec Measured defocus = -0.75 microns changed by -0.85 to target 34 frames are being saved to U:\krios\20190806\slot11_0707.tif 34 frames are being saved to U:\krios\20190806\slot11_0708.tif 34 frames are being saved to U:\krios\20190806\slot11_0709.tif Item 300: Stage: -800.52 623.64 70 Label: 298 300 Tick time from program start = 52927.894 Running script 17 at item 299 (-799.21, 621.26) Remaining time to fill dewars is 3780 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: 0.09 -0.29 Disparity in stage position after aligning to target position: 0.03 -0.35 (2nd move error -0.06 -0.09) Finished aligning to item in 2 rounds -14.5 24.8 unbinned pixels; 110.2 14.9 nm along two shift axes; -93.5 -61.7 nm on specimen axes Total_Shift = 57.523465 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 21.27, -12.85 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -5.2, 4.4 unbinned pixels, 0.12 nm/sec Measured defocus = -0.77 microns changed by -0.93 to target 34 frames are being saved to U:\krios\20190806\slot11_0710.tif 34 frames are being saved to U:\krios\20190806\slot11_0711.tif 34 frames are being saved to U:\krios\20190806\slot11_0712.tif Item 301: Stage: -799.21 621.26 70 Label: 299 301 Tick time from program start = 53049.903 Running script 17 at item 300 (-798.13, 618.86) Remaining time to fill dewars is 3660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.06 -0.35 (2nd move error -0.03 -0.08) Finished aligning to item in 1 rounds -10.3 6.3 unbinned pixels; 36.7 27.6 nm along two shift axes; -22.4 -41.4 nm on specimen axes Total_Shift = 24.17343 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 22.29, -13.26 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -10.7, 5.0 unbinned pixels, 0.21 nm/sec Measured defocus = -0.80 microns changed by -1.00 to target 34 frames are being saved to U:\krios\20190806\slot11_0713.tif 34 frames are being saved to U:\krios\20190806\slot11_0714.tif 34 frames are being saved to U:\krios\20190806\slot11_0715.tif Item 302: Stage: -798.13 618.86 70 Label: 300 302 Tick time from program start = 53161.709 Running script 17 at item 301 (-797.05, 616.53) Remaining time to fill dewars is 3540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.10 -0.36 (2nd move error 0.01 -0.09) Finished aligning to item in 1 rounds -12.9 -0.1 unbinned pixels; 16.3 46.8 nm along two shift axes; 3.3 -50.3 nm on specimen axes Total_Shift = 25.872701 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 20.43, -10.13 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.0, 5.2 unbinned pixels, 0.14 nm/sec Measured defocus = -0.70 microns changed by -1.20 to target 34 frames are being saved to U:\krios\20190806\slot11_0716.tif 34 frames are being saved to U:\krios\20190806\slot11_0717.tif 34 frames are being saved to U:\krios\20190806\slot11_0718.tif Item 303: Stage: -797.05 616.53 70 Label: 301 303 Tick time from program start = 53273.436 Running script 17 at item 302 (-795.94, 614.20) Remaining time to fill dewars is 3420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.20 -0.32 (2nd move error 0.11 -0.06) Finished aligning to item in 1 rounds -6.6 -2.6 unbinned pixels; 0.8 27.6 nm along two shift axes; 11.3 -25.1 nm on specimen axes Total_Shift = 14.137696 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 21.12, -11.32 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -4.2, 6.3 unbinned pixels, 0.14 nm/sec Measured defocus = -0.74 microns changed by -1.26 to target 34 frames are being saved to U:\krios\20190806\slot11_0719.tif 34 frames are being saved to U:\krios\20190806\slot11_0720.tif 34 frames are being saved to U:\krios\20190806\slot11_0721.tif Item 304: Stage: -795.94 614.20 70 Label: 302 304 Tick time from program start = 53385.398 Running script 17 at item 303 (-794.93, 611.93) Remaining time to fill dewars is 3300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.24 -0.32 (2nd move error 0.15 -0.06) Finished aligning to item in 1 rounds -5.5 0.3 unbinned pixels; 8.4 19.1 nm along two shift axes; -0.2 -21.3 nm on specimen axes Total_Shift = 10.933373 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 56.45, -36.36 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -2.0, 10.3 unbinned pixels, 0.19 nm/sec Measured defocus = -2.07 microns changed by -0.03 to target 34 frames are being saved to U:\krios\20190806\slot11_0722.tif 34 frames are being saved to U:\krios\20190806\slot11_0723.tif 34 frames are being saved to U:\krios\20190806\slot11_0724.tif Item 305: Stage: -794.93 611.93 70 Label: 303 305 Tick time from program start = 53497.672 Running script 17 at item 304 (-793.60, 609.56) Remaining time to fill dewars is 3180 Dewars are NOT busy filling Trimming A by 20 45 20 20, reference by 20 20 20 20 Trimming A by 20 45 20 20, reference by 20 20 20 20 Trimming A by 20 45 20 20, reference by 20 20 20 20 Trimming A by 20 45 20 20, reference by 24 100 20 90 Trimming A by 20 45 20 20, reference by 20 50 20 20 Trimming A by 20 45 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: 0.11 -0.31 Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: -0.00 -0.20 (2nd move error -0.12 0.10) Finished aligning to item in 2 rounds -5.6 28.0 unbinned pixels; 110.4 22.0 nm along two shift axes; -107.9 -27.8 nm on specimen axes Total_Shift = 57.17074 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 23.14, -13.73 unbinned pixels, unfiltered CCC is -0.0008 The average drift between shots was -8.0, 4.0 unbinned pixels, 0.16 nm/sec Measured defocus = -0.83 microns changed by -1.37 to target 34 frames are being saved to U:\krios\20190806\slot11_0725.tif 34 frames are being saved to U:\krios\20190806\slot11_0726.tif 34 frames are being saved to U:\krios\20190806\slot11_0727.tif Item 306: Stage: -793.60 609.56 70 Label: 304 306 Tick time from program start = 53617.606 Running script 17 at item 305 (-792.58, 607.19) Remaining time to fill dewars is 3060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.13 -0.23 (2nd move error 0.03 0.07) Finished aligning to item in 1 rounds -0.1 -2.7 unbinned pixels; 9.7 4.6 nm along two shift axes; 10.5 -0.0 nm on specimen axes Total_Shift = 5.380776 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 61.47, -40.30 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -9.1, 15.4 unbinned pixels, 0.32 nm/sec Measured defocus = -2.27 microns changed by -0.03 to target 34 frames are being saved to U:\krios\20190806\slot11_0728.tif 34 frames are being saved to U:\krios\20190806\slot11_0729.tif 34 frames are being saved to U:\krios\20190806\slot11_0730.tif Item 307: Stage: -792.58 607.19 70 Label: 305 307 Tick time from program start = 53729.895 Running script 17 at item 306 (-791.49, 604.82) Remaining time to fill dewars is 2940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.19 -0.22 (2nd move error 0.07 0.07) Finished aligning to item in 1 rounds 1.3 1.8 unbinned pixels; 5.1 7.4 nm along two shift axes; -7.4 4.6 nm on specimen axes Total_Shift = 4.504376 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 29.60, -19.41 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -12.2, 15.0 unbinned pixels, 0.34 nm/sec Measured defocus = -1.09 microns changed by -1.31 to target 34 frames are being saved to U:\krios\20190806\slot11_0731.tif 34 frames are being saved to U:\krios\20190806\slot11_0732.tif 34 frames are being saved to U:\krios\20190806\slot11_0733.tif Item 308: Stage: -791.49 604.82 70 Label: 306 308 Tick time from program start = 53841.358 Running script 17 at item 307 (-790.41, 602.47) Remaining time to fill dewars is 2760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 90 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.13 -0.19 (2nd move error 0.01 0.10) Finished aligning to item in 1 rounds Trimming A by 20 90 20 20, reference by 20 90 20 20 1.3 -0.7 unbinned pixels; 4.1 3.8 nm along two shift axes; 2.3 5.3 nm on specimen axes Total_Shift = 2.949061 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 65.14, -42.58 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -9.5, 11.0 unbinned pixels, 0.26 nm/sec Measured defocus = -2.40 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_0734.tif 34 frames are being saved to U:\krios\20190806\slot11_0735.tif 34 frames are being saved to U:\krios\20190806\slot11_0736.tif Item 309: Stage: -790.41 602.47 70 Label: 307 309 Tick time from program start = 53956.393 Running script 17 at item 308 (-792.64, 601.39) Remaining time to fill dewars is 2760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 118 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.18 -0.27 (2nd move error 0.07 0.03) Finished aligning to item in 1 rounds Trimming A by 20 118 20 20, reference by 20 118 20 20 0.9 -2.9 unbinned pixels; 12.0 1.0 nm along two shift axes; 11.2 4.2 nm on specimen axes Total_Shift = 6.134471 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -11.02, 8.62 unbinned pixels, unfiltered CCC is -0.0048 The average drift between shots was -7.6, 3.8 unbinned pixels, 0.15 nm/sec Measured defocus = 0.43 microns changed by -1.73 to target Tilt-induced shift is 33.60, -21.65 unbinned pixels, unfiltered CCC is -0.0011 The average drift between shots was -1.7, 3.5 unbinned pixels, 0.07 nm/sec Measured defocus = -1.24 microns changed by -0.06 to target 34 frames are being saved to U:\krios\20190806\slot11_0737.tif 34 frames are being saved to U:\krios\20190806\slot11_0738.tif 34 frames are being saved to U:\krios\20190806\slot11_0739.tif Item 310: Stage: -792.64 601.39 70 Label: 308 310 Tick time from program start = 54078.760 Running script 17 at item 309 (-793.75, 603.76) Remaining time to fill dewars is 2580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 40 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.10 -0.29 (2nd move error -0.01 -0.01) Finished aligning to item in 1 rounds Trimming A by 20 40 20 20, reference by 20 40 20 20 -2.1 3.0 unbinned pixels; 13.6 3.1 nm along two shift axes; -11.0 -8.8 nm on specimen axes Total_Shift = 7.234548 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -6.20, 5.42 unbinned pixels, unfiltered CCC is -0.0074 The average drift between shots was -7.9, 2.1 unbinned pixels, 0.15 nm/sec Measured defocus = 0.25 microns changed by -1.65 to target Tilt-induced shift is 35.40, -21.63 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -1.1, 2.1 unbinned pixels, 0.04 nm/sec Measured defocus = -1.28 microns changed by -0.12 to target 34 frames are being saved to U:\krios\20190806\slot11_0740.tif 34 frames are being saved to U:\krios\20190806\slot11_0741.tif 34 frames are being saved to U:\krios\20190806\slot11_0742.tif Item 311: Stage: -793.75 603.76 70 Label: 309 311 Tick time from program start = 54200.660 Running script 17 at item 310 (-794.80, 606.13) Remaining time to fill dewars is 2460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.13 -0.27 (2nd move error 0.01 0.02) Finished aligning to item in 1 rounds 1.2 -1.9 unbinned pixels; 8.7 1.5 nm along two shift axes; 7.3 5.2 nm on specimen axes Total_Shift = 4.598917 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is -2.13, 3.77 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -9.8, 4.2 unbinned pixels, 0.19 nm/sec Measured defocus = 0.12 microns changed by -1.62 to target Tilt-induced shift is 38.79, -25.34 unbinned pixels, unfiltered CCC is -0.0009 The average drift between shots was -1.5, 3.8 unbinned pixels, 0.07 nm/sec Measured defocus = -1.43 microns changed by -0.07 to target 34 frames are being saved to U:\krios\20190806\slot11_0743.tif 34 frames are being saved to U:\krios\20190806\slot11_0744.tif 34 frames are being saved to U:\krios\20190806\slot11_0745.tif Item 312: Stage: -794.80 606.13 70 Label: 310 312 Tick time from program start = 54322.060 Running script 17 at item 311 (-795.90, 608.49) Remaining time to fill dewars is 2280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.03 -0.26 (2nd move error -0.08 0.03) Finished aligning to item in 1 rounds -5.3 26.5 unbinned pixels; 104.4 21.0 nm along two shift axes; -102.1 -26.0 nm on specimen axes Total_Shift = 54.106839 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 2.87, 0.77 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -7.9, 0.9 unbinned pixels, 0.14 nm/sec Measured defocus = -0.06 microns changed by -1.54 to target Tilt-induced shift is 40.74, -25.94 unbinned pixels, unfiltered CCC is -0.0010 The average drift between shots was 0.4, 0.2 unbinned pixels, 0.01 nm/sec Measured defocus = -1.49 microns changed by -0.11 to target 34 frames are being saved to U:\krios\20190806\slot11_0746.tif 34 frames are being saved to U:\krios\20190806\slot11_0747.tif 34 frames are being saved to U:\krios\20190806\slot11_0748.tif Item 313: Stage: -795.90 608.49 70 Label: 311 313 Tick time from program start = 54447.422 Running script 17 at item 312 (-797.10, 610.90) Remaining time to fill dewars is 2160 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: 0.16 -0.29 Disparity in stage position after aligning to target position: 0.17 -0.36 (2nd move error 0.01 -0.09) Finished aligning to item in 2 rounds -11.3 -0.6 unbinned pixels; 12.6 41.6 nm along two shift axes; 4.6 -43.9 nm on specimen axes Total_Shift = 22.67505 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 45.25, -28.07 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.3, 5.9 unbinned pixels, 0.14 nm/sec Measured defocus = -1.65 microns changed by -0.05 to target 34 frames are being saved to U:\krios\20190806\slot11_0749.tif 34 frames are being saved to U:\krios\20190806\slot11_0750.tif 34 frames are being saved to U:\krios\20190806\slot11_0751.tif Item 314: Stage: -797.10 610.90 70 Label: 312 314 Tick time from program start = 54566.513 Running script 17 at item 313 (-798.19, 613.18) Remaining time to fill dewars is 2040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.15 -0.37 (2nd move error -0.01 -0.09) Finished aligning to item in 1 rounds -12.3 4.1 unbinned pixels; 31.2 38.1 nm along two shift axes; -13.5 -48.7 nm on specimen axes Total_Shift = 25.943547 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 10.83, -5.77 unbinned pixels, unfiltered CCC is -0.0010 The average drift between shots was -4.0, 3.9 unbinned pixels, 0.10 nm/sec Measured defocus = -0.38 microns changed by -1.42 to target 34 frames are being saved to U:\krios\20190806\slot11_0752.tif 34 frames are being saved to U:\krios\20190806\slot11_0753.tif 34 frames are being saved to U:\krios\20190806\slot11_0754.tif Item 315: Stage: -798.19 613.18 70 Label: 313 315 Tick time from program start = 54679.068 Running script 17 at item 314 (-799.28, 615.49) Remaining time to fill dewars is 1980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.16 -0.37 (2nd move error -0.00 -0.10) Finished aligning to item in 1 rounds -12.9 2.5 unbinned pixels; 25.9 42.6 nm along two shift axes; -7.0 -50.6 nm on specimen axes Total_Shift = 26.229149 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 14.29, -9.39 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -2.9, 4.2 unbinned pixels, 0.09 nm/sec Measured defocus = -0.53 microns changed by -1.37 to target 34 frames are being saved to U:\krios\20190806\slot11_0755.tif 34 frames are being saved to U:\krios\20190806\slot11_0756.tif 34 frames are being saved to U:\krios\20190806\slot11_0757.tif Item 316: Stage: -799.28 615.49 70 Label: 314 316 Tick time from program start = 54791.279 Running script 17 at item 315 (-800.37, 617.84) Remaining time to fill dewars is 1920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.09 -0.37 (2nd move error -0.07 -0.10) Finished aligning to item in 1 rounds -13.5 14.4 unbinned pixels; 70.6 26.8 nm along two shift axes; -53.2 -55.5 nm on specimen axes Total_Shift = 39.473384 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 54.80, -34.71 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -4.8, 5.4 unbinned pixels, 0.13 nm/sec Measured defocus = -2.01 microns changed by 0.01 to target 34 frames are being saved to U:\krios\20190806\slot11_0758.tif 34 frames are being saved to U:\krios\20190806\slot11_0759.tif 34 frames are being saved to U:\krios\20190806\slot11_0760.tif Item 317: Stage: -800.37 617.84 70 Label: 315 317 Tick time from program start = 54904.021 Running script 17 at item 316 (-801.61, 619.90) Remaining time to fill dewars is 1680 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to center of map frame: 0.03 -0.27 Disparity in stage position after aligning to target position: 0.27 -0.07 (2nd move error 0.23 0.18) Finished aligning to item in 2 rounds 0.8 13.9 unbinned pixels; 50.2 23.7 nm along two shift axes; -54.3 0.0 nm on specimen axes Total_Shift = 27.868752 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 28.66, -16.30 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -19.1, 7.9 unbinned pixels, 0.37 nm/sec Measured defocus = -1.02 microns changed by -1.08 to target 34 frames are being saved to U:\krios\20190806\slot11_0761.tif 34 frames are being saved to U:\krios\20190806\slot11_0762.tif 34 frames are being saved to U:\krios\20190806\slot11_0763.tif Item 318: Stage: -801.61 619.90 70 Label: 316 318 Tick time from program start = 55024.127 Running script 17 at item 317 (-802.63, 622.21) Remaining time to fill dewars is 1620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.23 -0.15 (2nd move error 0.19 0.10) Finished aligning to item in 1 rounds -0.0 -7.5 unbinned pixels; 27.6 11.3 nm along two shift axes; 29.2 1.6 nm on specimen axes Total_Shift = 15.02588 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 33.74, -21.44 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -9.8, 11.2 unbinned pixels, 0.27 nm/sec Measured defocus = -1.24 microns changed by -0.96 to target 34 frames are being saved to U:\krios\20190806\slot11_0764.tif 34 frames are being saved to U:\krios\20190806\slot11_0765.tif 34 frames are being saved to U:\krios\20190806\slot11_0766.tif Item 319: Stage: -802.63 622.21 70 Label: 317 319 Tick time from program start = 55136.510 Running script 17 at item 318 (-803.91, 624.64) Remaining time to fill dewars is 1500 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.08 -0.28 Disparity in stage position after aligning to target position: 0.28 -0.17 (2nd move error 0.19 0.08) Finished aligning to item in 2 rounds -3.3 0.5 unbinned pixels; 6.1 11.0 nm along two shift axes; -1.3 -12.8 nm on specimen axes Total_Shift = 6.591395 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 33.22, -22.37 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -5.1, 10.2 unbinned pixels, 0.20 nm/sec Measured defocus = -1.24 microns changed by -1.06 to target 34 frames are being saved to U:\krios\20190806\slot11_0767.tif 34 frames are being saved to U:\krios\20190806\slot11_0768.tif 34 frames are being saved to U:\krios\20190806\slot11_0769.tif Item 320: Stage: -803.91 624.64 70 Label: 318 320 Tick time from program start = 55258.362 Running script 17 at item 319 (-804.92, 626.91) Remaining time to fill dewars is 1260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.31 -0.24 (2nd move error 0.22 0.01) Finished aligning to item in 1 rounds 3.3 0.8 unbinned pixels; 1.4 13.0 nm along two shift axes; -3.7 12.6 nm on specimen axes Total_Shift = 6.767756 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 67.22, -48.61 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -4.0, 14.8 unbinned pixels, 0.27 nm/sec Measured defocus = -2.56 microns changed by 0.16 to target 34 frames are being saved to U:\krios\20190806\slot11_0770.tif 34 frames are being saved to U:\krios\20190806\slot11_0771.tif 34 frames are being saved to U:\krios\20190806\slot11_0772.tif Item 321: Stage: -804.92 626.91 70 Label: 319 321 Tick time from program start = 55370.730 Running script 17 at item 320 (-805.93, 629.24) Remaining time to fill dewars is 1260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.27 -0.26 (2nd move error 0.18 -0.00) Finished aligning to item in 1 rounds -0.5 -3.3 unbinned pixels; 11.6 6.7 nm along two shift axes; 13.0 -1.1 nm on specimen axes Total_Shift = 6.699158 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 35.47, -22.44 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -5.2, 15.0 unbinned pixels, 0.28 nm/sec Measured defocus = -1.30 microns changed by -1.20 to target 34 frames are being saved to U:\krios\20190806\slot11_0773.tif 34 frames are being saved to U:\krios\20190806\slot11_0774.tif 34 frames are being saved to U:\krios\20190806\slot11_0775.tif Item 322: Stage: -805.93 629.24 70 Label: 320 322 Tick time from program start = 55483.222 Running script 17 at item 321 (-807.01, 631.57) Remaining time to fill dewars is 1140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.11 -0.23 (2nd move error 0.02 0.02) Finished aligning to item in 1 rounds 0.0 -2.3 unbinned pixels; 8.4 3.3 nm along two shift axes; 8.8 0.6 nm on specimen axes Total_Shift = 4.516972 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 36.53, -21.29 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -7.5, 10.2 unbinned pixels, 0.22 nm/sec Measured defocus = -1.31 microns changed by 0.01 to target 34 frames are being saved to U:\krios\20190806\slot11_0776.tif 34 frames are being saved to U:\krios\20190806\slot11_0777.tif 34 frames are being saved to U:\krios\20190806\slot11_0778.tif Item 323: Stage: -807.01 631.57 70 Label: 321 323 Tick time from program start = 55595.231 Running script 17 at item 322 (-808.07, 633.93) Remaining time to fill dewars is 1020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.30 (2nd move error 0.10 -0.05) Finished aligning to item in 1 rounds -5.6 -2.9 unbinned pixels; 3.5 24.6 nm along two shift axes; 12.6 -21.2 nm on specimen axes Total_Shift = 12.647728 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -3.73, 2.55 unbinned pixels, unfiltered CCC is 0.0061 The average drift between shots was -3.7, 7.7 unbinned pixels, 0.15 nm/sec Measured defocus = 0.14 microns changed by -1.44 to target 34 frames are being saved to U:\krios\20190806\slot11_0779.tif 34 frames are being saved to U:\krios\20190806\slot11_0780.tif 34 frames are being saved to U:\krios\20190806\slot11_0781.tif Item 324: Stage: -808.07 633.93 70 Label: 322 324 Tick time from program start = 55707.770 Running script 17 at item 323 (-809.13, 636.27) Remaining time to fill dewars is 900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.09 -0.29 (2nd move error -0.00 -0.04) Finished aligning to item in 1 rounds -5.4 1.9 unbinned pixels; 14.0 16.7 nm along two shift axes; -6.2 -21.5 nm on specimen axes Total_Shift = 11.478867 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -7.30, 3.30 unbinned pixels, unfiltered CCC is 0.0041 The average drift between shots was 1.3, 5.2 unbinned pixels, 0.09 nm/sec Measured defocus = 0.25 microns changed by -1.65 to target Tilt-induced shift is 36.00, -23.22 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was 2.0, 3.4 unbinned pixels, 0.07 nm/sec Measured defocus = -1.32 microns changed by -0.08 to target 34 frames are being saved to U:\krios\20190806\slot11_0782.tif 34 frames are being saved to U:\krios\20190806\slot11_0783.tif 34 frames are being saved to U:\krios\20190806\slot11_0784.tif Item 325: Stage: -809.13 636.27 70 Label: 323 325 Tick time from program start = 55829.825 Running script 17 at item 324 (-810.48, 638.61) Remaining time to fill dewars is 720 Dewars are NOT busy filling Trimming A by 45 21 20 55, reference by 30 20 20 40 Trimming A by 45 21 20 55, reference by 20 20 20 20 Trimming A by 45 21 20 55, reference by 20 20 20 20 Trimming A by 45 21 20 55, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: 0.25 -0.30 Trimming A by 20 20 20 40, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.32 -0.34 (2nd move error 0.07 -0.06) Finished aligning to item in 2 rounds Trimming A by 20 20 20 35, reference by 20 20 20 40 -8.1 -2.8 unbinned pixels; 0.4 33.5 nm along two shift axes; 12.5 -31.0 nm on specimen axes Total_Shift = 17.18687 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is -5.23, 5.43 unbinned pixels, unfiltered CCC is 0.0136 The average drift between shots was -4.2, 6.6 unbinned pixels, 0.14 nm/sec Measured defocus = 0.23 microns changed by -1.73 to target Tilt-induced shift is 36.88, -24.16 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was 1.6, 4.2 unbinned pixels, 0.08 nm/sec Measured defocus = -1.36 microns changed by -0.14 to target 34 frames are being saved to U:\krios\20190806\slot11_0785.tif 34 frames are being saved to U:\krios\20190806\slot11_0786.tif 34 frames are being saved to U:\krios\20190806\slot11_0787.tif Item 326: Stage: -810.48 638.61 70 Label: 324 326 Tick time from program start = 55958.697 Running script 17 at item 325 (-811.53, 640.97) Remaining time to fill dewars is 600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 21 20 90, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.24 -0.35 (2nd move error -0.01 -0.07) Finished aligning to item in 1 rounds Trimming A by 21 21 20 90, reference by 21 21 20 90 -8.7 3.0 unbinned pixels; 22.6 26.9 nm along two shift axes; -9.9 -34.7 nm on specimen axes Total_Shift = 18.511632 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 34.82, -20.12 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -5.5, 4.8 unbinned pixels, 0.13 nm/sec Measured defocus = -1.24 microns changed by -0.36 to target 34 frames are being saved to U:\krios\20190806\slot11_0788.tif 34 frames are being saved to U:\krios\20190806\slot11_0789.tif 34 frames are being saved to U:\krios\20190806\slot11_0790.tif Item 327: Stage: -811.53 640.97 70 Label: 325 327 Tick time from program start = 56071.018 Running script 17 at item 326 (-812.65, 643.32) Remaining time to fill dewars is 540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 52 22 20 145, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.18 -0.36 (2nd move error -0.07 -0.08) Finished aligning to item in 1 rounds Trimming A by 52 22 20 145, reference by 52 22 20 145 -10.3 13.2 unbinned pixels; 62.1 17.1 nm along two shift axes; -49.3 -42.8 nm on specimen axes Total_Shift = 33.510832 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 8.82, -5.73 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -11.3, 6.3 unbinned pixels, 0.23 nm/sec Measured defocus = -0.33 microns changed by -1.37 to target 34 frames are being saved to U:\krios\20190806\slot11_0791.tif 34 frames are being saved to U:\krios\20190806\slot11_0792.tif 34 frames are being saved to U:\krios\20190806\slot11_0793.tif Item 328: Stage: -812.65 643.32 70 Label: 326 328 Tick time from program start = 56183.354 Running script 17 at item 327 (-813.80, 639.94) Remaining time to fill dewars is 360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 21 20 150, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.23 -0.39 (2nd move error -0.03 -0.11) Finished aligning to item in 1 rounds Trimming A by 21 21 20 150, reference by 21 21 20 150 -14.3 7.5 unbinned pixels; 46.0 40.1 nm along two shift axes; -26.0 -57.1 nm on specimen axes Total_Shift = 32.189935 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 1.83, 2.28 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -6.3, 0.9 unbinned pixels, 0.11 nm/sec Measured defocus = -0.01 microns changed by -1.79 to target Tilt-induced shift is 47.33, -29.98 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 0.9, 1.3 unbinned pixels, 0.03 nm/sec Measured defocus = -1.73 microns changed by -0.07 to target 34 frames are being saved to U:\krios\20190806\slot11_0794.tif 34 frames are being saved to U:\krios\20190806\slot11_0795.tif 34 frames are being saved to U:\krios\20190806\slot11_0796.tif Item 329: Stage: -813.80 639.94 70 Label: 327 329 Tick time from program start = 56305.956 Running script 17 at item 328 (-812.71, 637.65) Remaining time to fill dewars is 300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 110, reference by 30 20 20 40 Disparity in stage position after aligning to target position: 0.31 -0.40 (2nd move error 0.06 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 20 20 110, reference by 20 20 20 110 -12.3 -1.4 unbinned pixels; 10.8 46.2 nm along two shift axes; 8.1 -47.4 nm on specimen axes Total_Shift = 24.664586 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 38.38, -23.61 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -0.2, 6.6 unbinned pixels, 0.12 nm/sec Measured defocus = -1.39 microns changed by -0.51 to target 34 frames are being saved to U:\krios\20190806\slot11_0797.tif 34 frames are being saved to U:\krios\20190806\slot11_0798.tif 34 frames are being saved to U:\krios\20190806\slot11_0799.tif Item 330: Stage: -812.71 637.65 70 Label: 328 330 Tick time from program start = 56418.510 Running script 17 at item 329 (-811.40, 635.22) Remaining time to fill dewars is 240 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.20 -0.20 Trimming A by 20 20 20 70, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.07 -0.36 (2nd move error -0.14 -0.18) Finished aligning to item in 2 rounds Trimming A by 20 20 20 70, reference by 20 20 20 70 -21.9 27.4 unbinned pixels; 129.2 37.6 nm along two shift axes; -101.9 -90.9 nm on specimen axes Total_Shift = 70.080535 Trimming A by 20 20 20 70, reference by 20 20 20 70 -8.2 18.4 unbinned pixels; 78.4 1.6 nm along two shift axes; -69.9 -35.6 nm on specimen axes Total_Shift = 40.278996 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 7.21, -7.02 unbinned pixels, unfiltered CCC is 0.0032 The average drift between shots was 3.4, 4.6 unbinned pixels, 0.10 nm/sec Measured defocus = -0.30 microns changed by -1.70 to target Tilt-induced shift is 52.96, -34.30 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was 3.9, 0.9 unbinned pixels, 0.07 nm/sec Measured defocus = -1.95 microns changed by -0.05 to target 34 frames are being saved to U:\krios\20190806\slot11_0800.tif 34 frames are being saved to U:\krios\20190806\slot11_0801.tif 34 frames are being saved to U:\krios\20190806\slot11_0802.tif Item 331: Stage: -811.40 635.22 70 Label: 329 331 Tick time from program start = 56555.323 Running script 17 at item 330 (-810.32, 632.85) Remaining time to fill dewars is 120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 40, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.14 -0.32 (2nd move error -0.06 -0.14) Finished aligning to item in 1 rounds Trimming A by 20 20 20 40, reference by 20 20 20 40 -19.7 15.2 unbinned pixels; 81.8 48.1 nm along two shift axes; -55.1 -80.0 nm on specimen axes Total_Shift = 49.86508 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 56.88, -34.80 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was 5.9, 5.2 unbinned pixels, 0.14 nm/sec Measured defocus = -2.06 microns changed by -0.04 to target 34 frames are being saved to U:\krios\20190806\slot11_0803.tif 34 frames are being saved to U:\krios\20190806\slot11_0804.tif 34 frames are being saved to U:\krios\20190806\slot11_0805.tif Item 332: Stage: -810.32 632.85 70 Label: 330 332 Tick time from program start = 56671.669 Running script 17 at item 331 (-809.26, 630.53) Remaining time to fill dewars is -600 Dewars ARE busy filling Dewars ARE busy filling Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.21 -0.26 (2nd move error 0.01 -0.08) Finished aligning to item in 1 rounds -10.1 -0.1 unbinned pixels; 12.7 36.4 nm along two shift axes; 2.6 -39.1 nm on specimen axes Total_Shift = 20.127883 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 18.63, -12.88 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -4.8, 9.8 unbinned pixels, 0.20 nm/sec Measured defocus = -0.70 microns changed by -1.50 to target Tilt-induced shift is 58.21, -36.89 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was 1.3, 4.3 unbinned pixels, 0.08 nm/sec Measured defocus = -2.13 microns changed by -0.07 to target 34 frames are being saved to U:\krios\20190806\slot11_0806.tif 34 frames are being saved to U:\krios\20190806\slot11_0807.tif 34 frames are being saved to U:\krios\20190806\slot11_0808.tif Item 333: Stage: -809.26 630.53 70 Label: 331 333 Tick time from program start = 57153.369 Running script 17 at item 332 (-808.26, 628.19) Remaining time to fill dewars is 31860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.30 -0.23 (2nd move error 0.09 -0.06) Finished aligning to item in 1 rounds -6.8 -2.2 unbinned pixels; 0.8 27.9 nm along two shift axes; 10.0 -26.1 nm on specimen axes Total_Shift = 14.346051 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 57.44, -37.42 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -0.5, 11.9 unbinned pixels, 0.21 nm/sec Measured defocus = -2.12 microns changed by -0.18 to target 34 frames are being saved to U:\krios\20190806\slot11_0809.tif 34 frames are being saved to U:\krios\20190806\slot11_0810.tif 34 frames are being saved to U:\krios\20190806\slot11_0811.tif Item 334: Stage: -808.26 628.19 70 Label: 332 334 Tick time from program start = 57265.892 Running script 17 at item 333 (-807.15, 625.87) Remaining time to fill dewars is 31860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.21 -0.23 (2nd move error 0.01 -0.05) Finished aligning to item in 1 rounds -6.8 -1.0 unbinned pixels; 5.4 26.0 nm along two shift axes; 5.1 -26.3 nm on specimen axes Total_Shift = 13.752707 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 27.17, -19.32 unbinned pixels, unfiltered CCC is 0.0035 The average drift between shots was -0.0, 12.9 unbinned pixels, 0.23 nm/sec Measured defocus = -1.03 microns changed by -1.37 to target 34 frames are being saved to U:\krios\20190806\slot11_0812.tif 34 frames are being saved to U:\krios\20190806\slot11_0813.tif 34 frames are being saved to U:\krios\20190806\slot11_0814.tif Item 335: Stage: -807.15 625.87 70 Label: 333 335 Tick time from program start = 57378.104 Running script 17 at item 334 (-806.09, 623.63) Remaining time to fill dewars is 31680 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.24 -0.28 Disparity in stage position after aligning to target position: 0.36 -0.17 (2nd move error 0.12 0.09) Finished aligning to item in 2 rounds -2.3 -1.4 unbinned pixels; 2.2 10.4 nm along two shift axes; 6.0 -8.6 nm on specimen axes Total_Shift = 5.390242 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 70.56, -47.99 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -3.2, 14.3 unbinned pixels, 0.26 nm/sec Measured defocus = -2.63 microns changed by 0.13 to target 34 frames are being saved to U:\krios\20190806\slot11_0815.tif 34 frames are being saved to U:\krios\20190806\slot11_0816.tif 34 frames are being saved to U:\krios\20190806\slot11_0817.tif Item 336: Stage: -806.09 623.63 70 Label: 334 336 Tick time from program start = 57499.613 Running script 17 at item 335 (-804.91, 621.20) Remaining time to fill dewars is 31560 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to center of map frame: 0.20 -0.30 Disparity in stage position after aligning to target position: 0.25 -0.14 (2nd move error 0.04 0.13) Finished aligning to item in 2 rounds 1.1 1.7 unbinned pixels; 4.9 6.4 nm along two shift axes; -6.8 3.7 nm on specimen axes Total_Shift = 3.999384 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 2.48, 0.85 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -11.3, 8.4 unbinned pixels, 0.25 nm/sec Measured defocus = -0.05 microns changed by -1.25 to target 34 frames are being saved to U:\krios\20190806\slot11_0818.tif 34 frames are being saved to U:\krios\20190806\slot11_0819.tif 34 frames are being saved to U:\krios\20190806\slot11_0820.tif Item 337: Stage: -804.91 621.20 70 Label: 335 337 Tick time from program start = 57621.309 Running script 17 at item 336 (-803.85, 618.84) Remaining time to fill dewars is 31560 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.15 (2nd move error -0.02 0.12) Finished aligning to item in 1 rounds -3.0 12.8 unbinned pixels; 50.9 8.6 nm along two shift axes; -49.1 -14.2 nm on specimen axes Total_Shift = 26.249809 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -0.77, 1.54 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -10.7, 5.0 unbinned pixels, 0.21 nm/sec Measured defocus = 0.04 microns changed by -1.44 to target 34 frames are being saved to U:\krios\20190806\slot11_0821.tif 34 frames are being saved to U:\krios\20190806\slot11_0822.tif 34 frames are being saved to U:\krios\20190806\slot11_0823.tif Item 338: Stage: -803.85 618.84 70 Label: 336 338 Tick time from program start = 57753.926 Running script 17 at item 337 (-802.77, 616.54) Remaining time to fill dewars is 31440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.32 -0.16 (2nd move error 0.11 0.12) Finished aligning to item in 1 rounds 2.0 -2.8 unbinned pixels; 12.8 3.0 nm along two shift axes; 10.3 8.3 nm on specimen axes Total_Shift = 6.820449 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is -0.20, 1.52 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -8.4, 8.2 unbinned pixels, 0.21 nm/sec Measured defocus = 0.03 microns changed by -1.53 to target Tilt-induced shift is 37.40, -24.54 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -2.2, 6.2 unbinned pixels, 0.12 nm/sec Measured defocus = -1.38 microns changed by -0.12 to target 34 frames are being saved to U:\krios\20190806\slot11_0824.tif 34 frames are being saved to U:\krios\20190806\slot11_0825.tif 34 frames are being saved to U:\krios\20190806\slot11_0826.tif Item 339: Stage: -802.77 616.54 70 Label: 337 339 Tick time from program start = 57876.090 Running script 17 at item 338 (-801.71, 614.21) Remaining time to fill dewars is 31380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.34 -0.15 (2nd move error 0.13 0.12) Finished aligning to item in 1 rounds 2.0 8.9 unbinned pixels; 30.2 20.6 nm along two shift axes; -35.1 5.9 nm on specimen axes Total_Shift = 18.260533 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is -1.15, 2.22 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -1.1, 1.0 unbinned pixels, 0.03 nm/sec Measured defocus = 0.07 microns changed by -1.67 to target Tilt-induced shift is 46.62, -30.33 unbinned pixels, unfiltered CCC is -0.0009 The average drift between shots was -3.2, 3.3 unbinned pixels, 0.08 nm/sec Measured defocus = -1.72 microns changed by 0.12 to target 34 frames are being saved to U:\krios\20190806\slot11_0827.tif 34 frames are being saved to U:\krios\20190806\slot11_0828.tif 34 frames are being saved to U:\krios\20190806\slot11_0829.tif Item 340: Stage: -801.71 614.21 70 Label: 338 340 Tick time from program start = 57997.942 Running script 17 at item 339 (-800.54, 612.15) Remaining time to fill dewars is 31260 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.25 Disparity in stage position after aligning to target position: 0.04 -0.42 (2nd move error -0.00 -0.19) Finished aligning to item in 2 rounds -23.8 3.1 unbinned pixels; 42.5 81.1 nm along two shift axes; -7.1 -93.4 nm on specimen axes Total_Shift = 48.076791 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 7.01, -6.10 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -2.6, 4.2 unbinned pixels, 0.09 nm/sec Measured defocus = -0.28 microns changed by -1.42 to target 34 frames are being saved to U:\krios\20190806\slot11_0830.tif 34 frames are being saved to U:\krios\20190806\slot11_0831.tif 34 frames are being saved to U:\krios\20190806\slot11_0832.tif Item 341: Stage: -800.54 612.15 70 Label: 339 341 Tick time from program start = 58117.985 Running script 17 at item 340 (-799.24, 609.81) Remaining time to fill dewars is 31140 Dewars are NOT busy filling Trimming A by 20 45 20 20, reference by 20 20 20 20 Trimming A by 20 45 20 20, reference by 20 20 20 20 Trimming A by 20 45 20 20, reference by 20 20 20 20 Trimming A by 20 45 20 20, reference by 24 100 20 90 Trimming A by 20 45 20 20, reference by 20 50 20 20 Trimming A by 20 45 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to center of map frame: -0.00 -0.28 Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: -0.12 -0.27 (2nd move error -0.12 -0.02) Finished aligning to item in 2 rounds -2.3 23.8 unbinned pixels; 90.7 27.6 nm along two shift axes; -92.3 -14.0 nm on specimen axes Total_Shift = 47.920975 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 10.24, -5.78 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -5.1, 1.3 unbinned pixels, 0.09 nm/sec Measured defocus = -0.36 microns changed by -1.44 to target 34 frames are being saved to U:\krios\20190806\slot11_0833.tif 34 frames are being saved to U:\krios\20190806\slot11_0834.tif 34 frames are being saved to U:\krios\20190806\slot11_0835.tif Item 342: Stage: -799.24 609.81 70 Label: 340 342 Tick time from program start = 58240.836 Running script 17 at item 341 (-798.09, 607.43) Remaining time to fill dewars is 31020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: -0.02 -0.32 (2nd move error -0.02 -0.07) Finished aligning to item in 1 rounds -8.9 5.3 unbinned pixels; 31.0 23.9 nm along two shift axes; -18.7 -35.6 nm on specimen axes Total_Shift = 20.633895 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 45.61, -29.12 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -5.2, 3.0 unbinned pixels, 0.10 nm/sec Measured defocus = -1.67 microns changed by -0.23 to target 34 frames are being saved to U:\krios\20190806\slot11_0836.tif 34 frames are being saved to U:\krios\20190806\slot11_0837.tif 34 frames are being saved to U:\krios\20190806\slot11_0838.tif Item 343: Stage: -798.09 607.43 70 Label: 341 343 Tick time from program start = 58352.876 Running script 17 at item 342 (-797.04, 605.08) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.04 -0.31 (2nd move error 0.04 -0.05) Finished aligning to item in 1 rounds -5.8 -2.2 unbinned pixels; 0.5 24.2 nm along two shift axes; 9.8 -22.1 nm on specimen axes Total_Shift = 12.426472 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 10.55, -5.43 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -6.1, 5.1 unbinned pixels, 0.14 nm/sec Measured defocus = -0.37 microns changed by -1.63 to target Tilt-induced shift is 51.61, -33.14 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 1.2, 4.6 unbinned pixels, 0.09 nm/sec Measured defocus = -1.90 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_0839.tif 34 frames are being saved to U:\krios\20190806\slot11_0840.tif 34 frames are being saved to U:\krios\20190806\slot11_0841.tif Item 344: Stage: -797.04 605.08 70 Label: 342 344 Tick time from program start = 58474.510 Running script 17 at item 343 (-795.97, 602.68) Remaining time to fill dewars is 30960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 70 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.09 -0.29 (2nd move error 0.09 -0.04) Finished aligning to item in 1 rounds Trimming A by 20 70 20 20, reference by 20 70 20 20 -4.2 -2.2 unbinned pixels; 2.7 18.6 nm along two shift axes; 9.6 -16.0 nm on specimen axes Total_Shift = 9.582981 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 44.62, -27.52 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -2.7, 7.8 unbinned pixels, 0.15 nm/sec Measured defocus = -1.62 microns changed by -0.48 to target 34 frames are being saved to U:\krios\20190806\slot11_0842.tif 34 frames are being saved to U:\krios\20190806\slot11_0843.tif 34 frames are being saved to U:\krios\20190806\slot11_0844.tif Item 345: Stage: -795.97 602.68 70 Label: 343 345 Tick time from program start = 58586.862 Running script 17 at item 344 (-798.21, 601.62) Remaining time to fill dewars is 30840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 100 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.10 -0.35 (2nd move error 0.09 -0.09) Finished aligning to item in 1 rounds Trimming A by 20 100 20 35, reference by 20 100 20 20 -10.9 -1.1 unbinned pixels; 10.2 40.7 nm along two shift axes; 6.5 -42.0 nm on specimen axes Total_Shift = 21.822059 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 14.21, -8.84 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -4.8, 6.4 unbinned pixels, 0.14 nm/sec Measured defocus = -0.52 microns changed by -1.68 to target Tilt-induced shift is 57.22, -36.51 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was 1.8, 5.0 unbinned pixels, 0.09 nm/sec Measured defocus = -2.10 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_0845.tif 34 frames are being saved to U:\krios\20190806\slot11_0846.tif 34 frames are being saved to U:\krios\20190806\slot11_0847.tif Item 346: Stage: -798.21 601.62 70 Label: 344 346 Tick time from program start = 58709.525 Running script 17 at item 345 (-799.30, 604.01) Remaining time to fill dewars is 30720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 50 20 45, reference by 20 50 20 20 Disparity in stage position after aligning to target position: 0.10 -0.37 (2nd move error 0.09 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 27 20 50, reference by 20 50 20 45 -13.8 -0.7 unbinned pixels; 15.4 50.6 nm along two shift axes; 5.6 -53.4 nm on specimen axes Total_Shift = 27.575015 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 56.69, -36.42 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was -3.2, 8.6 unbinned pixels, 0.16 nm/sec Measured defocus = -2.08 microns changed by -0.22 to target 34 frames are being saved to U:\krios\20190806\slot11_0848.tif 34 frames are being saved to U:\krios\20190806\slot11_0849.tif 34 frames are being saved to U:\krios\20190806\slot11_0850.tif Item 347: Stage: -799.30 604.01 70 Label: 345 347 Tick time from program start = 58822.470 Running script 17 at item 346 (-800.50, 606.12) Remaining time to fill dewars is 30660 Dewars are NOT busy filling Trimming A by 24 100 20 90, reference by 20 20 20 20 Trimming A by 24 100 20 90, reference by 20 20 20 20 Trimming A by 24 100 20 90, reference by 24 100 20 90 Trimming A by 24 100 20 90, reference by 20 50 20 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.26 Trimming A by 20 20 20 20, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.18 -0.10 (2nd move error 0.14 0.14) Finished aligning to item in 2 rounds 0.5 -6.8 unbinned pixels; 25.8 8.3 nm along two shift axes; 26.4 3.5 nm on specimen axes Total_Shift = 13.692799 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 23.80, -13.94 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -9.3, 10.6 unbinned pixels, 0.25 nm/sec Measured defocus = -0.85 microns changed by -1.55 to target Tilt-induced shift is 62.06, -39.11 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -1.3, 4.6 unbinned pixels, 0.09 nm/sec Measured defocus = -2.27 microns changed by -0.13 to target 34 frames are being saved to U:\krios\20190806\slot11_0851.tif 34 frames are being saved to U:\krios\20190806\slot11_0852.tif 34 frames are being saved to U:\krios\20190806\slot11_0853.tif Item 348: Stage: -800.50 606.12 70 Label: 346 348 Tick time from program start = 58952.544 Running script 17 at item 347 (-801.54, 608.47) Remaining time to fill dewars is 30540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.20 -0.18 (2nd move error 0.15 0.06) Finished aligning to item in 1 rounds -0.7 6.4 unbinned pixels; 24.5 7.3 nm along two shift axes; -24.8 -3.9 nm on specimen axes Total_Shift = 12.90535 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 64.96, -42.18 unbinned pixels, unfiltered CCC is -0.0010 The average drift between shots was -5.6, 6.9 unbinned pixels, 0.16 nm/sec Measured defocus = -2.39 microns changed by -0.11 to target 34 frames are being saved to U:\krios\20190806\slot11_0854.tif 34 frames are being saved to U:\krios\20190806\slot11_0855.tif 34 frames are being saved to U:\krios\20190806\slot11_0856.tif Item 349: Stage: -801.54 608.47 70 Label: 347 349 Tick time from program start = 59065.847 Running script 17 at item 348 (-802.85, 610.84) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to center of map frame: 0.04 -0.27 Disparity in stage position after aligning to target position: 0.37 -0.14 (2nd move error 0.32 0.10) Finished aligning to item in 2 rounds -0.5 -6.9 unbinned pixels; 24.6 12.2 nm along two shift axes; 26.8 -0.5 nm on specimen axes Total_Shift = 13.783137 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -7.31, 3.98 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -8.8, 9.6 unbinned pixels, 0.23 nm/sec Measured defocus = 0.26 microns changed by -1.56 to target Tilt-induced shift is 31.94, -20.78 unbinned pixels, unfiltered CCC is -0.0018 The average drift between shots was -1.8, 5.3 unbinned pixels, 0.10 nm/sec Measured defocus = -1.18 microns changed by -0.12 to target 34 frames are being saved to U:\krios\20190806\slot11_0857.tif 34 frames are being saved to U:\krios\20190806\slot11_0858.tif 34 frames are being saved to U:\krios\20190806\slot11_0859.tif Item 350: Stage: -802.85 610.84 70 Label: 348 350 Tick time from program start = 59197.964 Running script 17 at item 349 (-803.90, 613.15) Remaining time to fill dewars is 30420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.28 -0.20 (2nd move error 0.23 0.04) Finished aligning to item in 1 rounds 1.2 1.2 unbinned pixels; 2.8 6.0 nm along two shift axes; -4.9 4.3 nm on specimen axes Total_Shift = 3.337566 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -8.39, 5.27 unbinned pixels, unfiltered CCC is -0.0038 The average drift between shots was -6.6, 8.2 unbinned pixels, 0.19 nm/sec Measured defocus = 0.31 microns changed by -1.61 to target Tilt-induced shift is 33.02, -21.36 unbinned pixels, unfiltered CCC is -0.0009 The average drift between shots was -1.9, 4.7 unbinned pixels, 0.09 nm/sec Measured defocus = -1.22 microns changed by -0.08 to target 34 frames are being saved to U:\krios\20190806\slot11_0860.tif 34 frames are being saved to U:\krios\20190806\slot11_0861.tif 34 frames are being saved to U:\krios\20190806\slot11_0862.tif Item 351: Stage: -803.90 613.15 70 Label: 349 351 Tick time from program start = 59319.801 Running script 17 at item 350 (-804.97, 615.41) Remaining time to fill dewars is 30300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.31 -0.22 (2nd move error 0.27 0.02) Finished aligning to item in 1 rounds 2.4 -1.6 unbinned pixels; 9.0 6.2 nm along two shift axes; 5.8 9.6 nm on specimen axes Total_Shift = 5.757749 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -7.20, 6.73 unbinned pixels, unfiltered CCC is -0.0047 The average drift between shots was -9.5, 5.7 unbinned pixels, 0.19 nm/sec Measured defocus = 0.30 microns changed by -1.70 to target Tilt-induced shift is 35.95, -23.04 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -2.2, 4.2 unbinned pixels, 0.08 nm/sec Measured defocus = -1.32 microns changed by -0.08 to target 34 frames are being saved to U:\krios\20190806\slot11_0863.tif 34 frames are being saved to U:\krios\20190806\slot11_0864.tif 34 frames are being saved to U:\krios\20190806\slot11_0865.tif Item 352: Stage: -804.97 615.41 70 Label: 350 352 Tick time from program start = 59442.356 Running script 17 at item 351 (-806.01, 617.76) Remaining time to fill dewars is 30240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.24 -0.22 (2nd move error 0.19 0.03) Finished aligning to item in 1 rounds -0.8 -5.8 unbinned pixels; 20.1 11.7 nm along two shift axes; 22.6 -2.0 nm on specimen axes Total_Shift = 11.668543 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 0.43, 0.51 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -12.6, 8.9 unbinned pixels, 0.28 nm/sec Measured defocus = -0.00 microns changed by -1.50 to target 34 frames are being saved to U:\krios\20190806\slot11_0866.tif 34 frames are being saved to U:\krios\20190806\slot11_0867.tif 34 frames are being saved to U:\krios\20190806\slot11_0868.tif Item 353: Stage: -806.01 617.76 70 Label: 351 353 Tick time from program start = 59555.300 Running script 17 at item 352 (-807.11, 620.16) Remaining time to fill dewars is 30120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.10 -0.24 (2nd move error 0.05 0.01) Finished aligning to item in 1 rounds 0.4 -1.9 unbinned pixels; 7.4 1.3 nm along two shift axes; 7.2 2.0 nm on specimen axes Total_Shift = 3.840151 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 0.40, 2.77 unbinned pixels, unfiltered CCC is 0.0070 The average drift between shots was -0.4, 3.1 unbinned pixels, 0.06 nm/sec Measured defocus = 0.03 microns changed by -1.63 to target Tilt-induced shift is 46.53, -28.57 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -0.3, 4.1 unbinned pixels, 0.07 nm/sec Measured defocus = -1.69 microns changed by 0.09 to target 34 frames are being saved to U:\krios\20190806\slot11_0869.tif 34 frames are being saved to U:\krios\20190806\slot11_0870.tif 34 frames are being saved to U:\krios\20190806\slot11_0871.tif Item 354: Stage: -807.11 620.16 70 Label: 352 354 Tick time from program start = 59676.794 Running script 17 at item 353 (-808.19, 622.50) Remaining time to fill dewars is 30000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.16 -0.26 (2nd move error 0.11 -0.01) Finished aligning to item in 1 rounds -2.4 -7.2 unbinned pixels; 23.3 19.6 nm along two shift axes; 28.6 -8.0 nm on specimen axes Total_Shift = 15.217571 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 6.93, -7.60 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was -2.8, 10.7 unbinned pixels, 0.20 nm/sec Measured defocus = -0.31 microns changed by -1.39 to target 34 frames are being saved to U:\krios\20190806\slot11_0872.tif 34 frames are being saved to U:\krios\20190806\slot11_0873.tif 34 frames are being saved to U:\krios\20190806\slot11_0874.tif Item 355: Stage: -808.19 622.50 70 Label: 353 355 Tick time from program start = 59789.239 Running script 17 at item 354 (-809.39, 624.85) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 20 40 Disparity in stage position after aligning to center of map frame: 0.20 -0.27 Trimming A by 20 20 20 35, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.17 -0.34 (2nd move error -0.02 -0.11) Finished aligning to item in 2 rounds Trimming A by 20 20 20 35, reference by 20 20 20 35 -12.4 6.5 unbinned pixels; 40.1 34.8 nm along two shift axes; -22.7 -49.6 nm on specimen axes Total_Shift = 27.977269 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 8.48, -5.91 unbinned pixels, unfiltered CCC is 0.0046 The average drift between shots was -2.6, 6.9 unbinned pixels, 0.13 nm/sec Measured defocus = -0.32 microns changed by -1.48 to target 34 frames are being saved to U:\krios\20190806\slot11_0875.tif 34 frames are being saved to U:\krios\20190806\slot11_0876.tif 34 frames are being saved to U:\krios\20190806\slot11_0877.tif Item 356: Stage: -809.39 624.85 70 Label: 354 356 Tick time from program start = 59908.471 Running script 17 at item 355 (-810.40, 627.11) Remaining time to fill dewars is 29880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 60, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.14 -0.34 (2nd move error -0.06 -0.09) Finished aligning to item in 1 rounds Trimming A by 20 20 20 60, reference by 20 20 20 60 -11.2 12.0 unbinned pixels; 58.9 22.1 nm along two shift axes; -44.5 -46.0 nm on specimen axes Total_Shift = 32.862353 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 40.18, -26.24 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -2.1, 5.0 unbinned pixels, 0.10 nm/sec Measured defocus = -1.48 microns changed by -0.42 to target 34 frames are being saved to U:\krios\20190806\slot11_0878.tif 34 frames are being saved to U:\krios\20190806\slot11_0879.tif 34 frames are being saved to U:\krios\20190806\slot11_0880.tif Item 357: Stage: -810.40 627.11 70 Label: 355 357 Tick time from program start = 60020.823 Running script 17 at item 356 (-811.51, 629.41) Remaining time to fill dewars is 29700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 90, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.10 -0.35 (2nd move error -0.11 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 20 20 90, reference by 20 20 20 90 -14.6 22.0 unbinned pixels; 99.8 19.3 nm along two shift axes; -82.4 -61.2 nm on specimen axes Total_Shift = 52.699426 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 10.78, -5.55 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -7.3, 4.3 unbinned pixels, 0.15 nm/sec Measured defocus = -0.37 microns changed by -1.63 to target Tilt-induced shift is 51.29, -32.29 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -0.1, 1.0 unbinned pixels, 0.02 nm/sec Measured defocus = -1.87 microns changed by -0.13 to target 34 frames are being saved to U:\krios\20190806\slot11_0881.tif 34 frames are being saved to U:\krios\20190806\slot11_0882.tif 34 frames are being saved to U:\krios\20190806\slot11_0883.tif Item 358: Stage: -811.51 629.41 70 Label: 356 358 Tick time from program start = 60143.440 Running script 17 at item 357 (-812.57, 631.82) Remaining time to fill dewars is 29700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 115, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.06 -0.35 (2nd move error -0.14 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 20 20 115, reference by 20 20 20 115 -14.7 27.8 unbinned pixels; 121.5 10.9 nm along two shift axes; -105.2 -62.9 nm on specimen axes Total_Shift = 62.92919 Trimming A by 20 20 20 120, reference by 20 20 20 115 -8.3 18.5 unbinned pixels; 78.9 2.1 nm along two shift axes; -70.3 -36.3 nm on specimen axes Total_Shift = 40.598901 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 46.91, -31.35 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -3.7, 7.8 unbinned pixels, 0.15 nm/sec Measured defocus = -1.74 microns changed by -0.36 to target 34 frames are being saved to U:\krios\20190806\slot11_0884.tif 34 frames are being saved to U:\krios\20190806\slot11_0885.tif 34 frames are being saved to U:\krios\20190806\slot11_0886.tif Item 359: Stage: -812.57 631.82 70 Label: 357 359 Tick time from program start = 60261.844 Running script 17 at item 358 (-812.64, 626.08) Remaining time to fill dewars is 29580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 135, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.15 -0.34 (2nd move error -0.05 -0.10) Finished aligning to item in 1 rounds Trimming A by 20 20 20 135, reference by 20 20 20 135 -13.6 11.3 unbinned pixels; 59.1 32.0 nm along two shift axes; -40.9 -55.3 nm on specimen axes Total_Shift = 35.292498 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 10.74, -7.08 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -5.2, 2.1 unbinned pixels, 0.10 nm/sec Measured defocus = -0.40 microns changed by -1.80 to target Tilt-induced shift is 57.11, -35.95 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 0.1, 0.2 unbinned pixels, 0.00 nm/sec Measured defocus = -2.09 microns changed by -0.11 to target 34 frames are being saved to U:\krios\20190806\slot11_0887.tif 34 frames are being saved to U:\krios\20190806\slot11_0888.tif 34 frames are being saved to U:\krios\20190806\slot11_0889.tif Item 360: Stage: -812.64 626.08 70 Label: 358 360 Tick time from program start = 60383.978 Running script 17 at item 359 (-811.60, 623.80) Remaining time to fill dewars is 29520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 110, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.22 -0.33 (2nd move error 0.01 -0.09) Finished aligning to item in 1 rounds Trimming A by 20 20 20 110, reference by 20 20 20 110 -11.4 -1.2 unbinned pixels; 10.5 42.9 nm along two shift axes; 7.1 -44.2 nm on specimen axes Total_Shift = 22.957558 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 52.65, -35.58 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -0.2, 11.0 unbinned pixels, 0.20 nm/sec Measured defocus = -1.96 microns changed by -0.34 to target 34 frames are being saved to U:\krios\20190806\slot11_0890.tif 34 frames are being saved to U:\krios\20190806\slot11_0891.tif 34 frames are being saved to U:\krios\20190806\slot11_0892.tif Item 361: Stage: -811.60 623.80 70 Label: 359 361 Tick time from program start = 60496.392 Running script 17 at item 360 (-810.43, 621.43) Remaining time to fill dewars is 29400 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.20 Trimming A by 20 20 20 80, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.07 -0.30 (2nd move error -0.06 -0.11) Finished aligning to item in 2 rounds Trimming A by 20 20 20 80, reference by 20 20 20 80 -19.1 18.9 unbinned pixels; 94.3 40.2 nm along two shift axes; -69.4 -78.2 nm on specimen axes Total_Shift = 53.682918 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 23.24, -17.78 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -4.3, 8.0 unbinned pixels, 0.16 nm/sec Measured defocus = -0.90 microns changed by -1.50 to target Tilt-induced shift is 62.59, -38.67 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 2.0, 0.8 unbinned pixels, 0.04 nm/sec Measured defocus = -2.27 microns changed by -0.13 to target 34 frames are being saved to U:\krios\20190806\slot11_0893.tif 34 frames are being saved to U:\krios\20190806\slot11_0894.tif 34 frames are being saved to U:\krios\20190806\slot11_0895.tif Item 362: Stage: -810.43 621.43 70 Label: 360 362 Tick time from program start = 60628.353 Running script 17 at item 361 (-809.32, 619.02) Remaining time to fill dewars is 29280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 50, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.13 -0.26 (2nd move error 0.00 -0.08) Finished aligning to item in 1 rounds Trimming A by 20 20 20 50, reference by 20 20 20 50 -10.6 0.9 unbinned pixels; 17.0 36.9 nm along two shift axes; -1.1 -41.5 nm on specimen axes Total_Shift = 21.307032 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 59.82, -39.51 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -4.3, 10.8 unbinned pixels, 0.21 nm/sec Measured defocus = -2.21 microns changed by -0.29 to target 34 frames are being saved to U:\krios\20190806\slot11_0896.tif 34 frames are being saved to U:\krios\20190806\slot11_0897.tif 34 frames are being saved to U:\krios\20190806\slot11_0898.tif Item 363: Stage: -809.32 619.02 70 Label: 361 363 Tick time from program start = 60740.908 Running script 17 at item 362 (-808.27, 616.69) Remaining time to fill dewars is 29280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 30, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.22 -0.27 (2nd move error 0.10 -0.07) Finished aligning to item in 1 rounds Trimming A by 20 20 20 25, reference by 20 20 20 30 -9.0 -5.6 unbinned pixels; 8.8 41.0 nm along two shift axes; 23.7 -34.0 nm on specimen axes Total_Shift = 21.279343 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -11.49, 7.78 unbinned pixels, unfiltered CCC is 0.0075 The average drift between shots was -7.2, 8.4 unbinned pixels, 0.20 nm/sec Measured defocus = 0.43 microns changed by -1.73 to target Tilt-induced shift is 30.21, -20.43 unbinned pixels, unfiltered CCC is 0.0031 The average drift between shots was 2.0, 5.6 unbinned pixels, 0.10 nm/sec Measured defocus = -1.13 microns changed by -0.17 to target 34 frames are being saved to U:\krios\20190806\slot11_0899.tif 34 frames are being saved to U:\krios\20190806\slot11_0900.tif 34 frames are being saved to U:\krios\20190806\slot11_0901.tif Item 364: Stage: -808.27 616.69 70 Label: 362 364 Tick time from program start = 60862.776 Running script 17 at item 363 (-807.17, 614.35) Remaining time to fill dewars is 29160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.19 -0.22 (2nd move error 0.07 -0.04) Finished aligning to item in 1 rounds -3.6 -0.5 unbinned pixels; 2.9 13.8 nm along two shift axes; 2.7 -14.0 nm on specimen axes Total_Shift = 7.323832 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -10.76, 8.97 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was -4.3, 4.7 unbinned pixels, 0.11 nm/sec Measured defocus = 0.43 microns changed by -1.83 to target Tilt-induced shift is 36.65, -18.03 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -0.1, -2.6 unbinned pixels, 0.05 nm/sec Measured defocus = -1.26 microns changed by -0.14 to target 34 frames are being saved to U:\krios\20190806\slot11_0902.tif 34 frames are being saved to U:\krios\20190806\slot11_0903.tif 34 frames are being saved to U:\krios\20190806\slot11_0904.tif Item 365: Stage: -807.17 614.35 70 Label: 363 365 Tick time from program start = 60984.675 Running script 17 at item 364 (-806.14, 612.08) Remaining time to fill dewars is 29100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.31 -0.23 (2nd move error 0.18 -0.04) Finished aligning to item in 1 rounds -3.7 -3.1 unbinned pixels; 6.7 18.0 nm along two shift axes; 13.0 -13.7 nm on specimen axes Total_Shift = 9.68159 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 30.50, -21.44 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -0.0, 11.9 unbinned pixels, 0.21 nm/sec Measured defocus = -1.15 microns changed by -0.35 to target 34 frames are being saved to U:\krios\20190806\slot11_0905.tif 34 frames are being saved to U:\krios\20190806\slot11_0906.tif 34 frames are being saved to U:\krios\20190806\slot11_0907.tif Item 366: Stage: -806.14 612.08 70 Label: 364 366 Tick time from program start = 61097.043 Running script 17 at item 365 (-805.05, 609.79) Remaining time to fill dewars is 28860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: 0.40 -0.19 (2nd move error 0.27 -0.01) Finished aligning to item in 1 rounds 0.8 3.2 unbinned pixels; 10.5 7.8 nm along two shift axes; -12.5 2.6 nm on specimen axes Total_Shift = 6.556258 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is -1.65, 0.57 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -6.8, 7.1 unbinned pixels, 0.17 nm/sec Measured defocus = 0.05 microns changed by -1.65 to target Tilt-induced shift is 41.92, -26.81 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -0.2, 3.9 unbinned pixels, 0.07 nm/sec Measured defocus = -1.54 microns changed by -0.06 to target 34 frames are being saved to U:\krios\20190806\slot11_0908.tif 34 frames are being saved to U:\krios\20190806\slot11_0909.tif 34 frames are being saved to U:\krios\20190806\slot11_0910.tif Item 367: Stage: -805.05 609.79 70 Label: 365 367 Tick time from program start = 61218.989 Running script 17 at item 366 (-803.77, 607.42) Remaining time to fill dewars is 28860 Dewars are NOT busy filling Trimming A by 20 100 20 95, reference by 20 20 20 20 Trimming A by 20 100 20 95, reference by 20 20 20 20 Trimming A by 20 100 20 95, reference by 24 100 20 90 Trimming A by 20 100 20 95, reference by 20 50 20 20 Disparity in stage position after aligning to center of map frame: 0.15 -0.28 Trimming A by 20 20 20 20, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.11 -0.15 (2nd move error -0.04 0.12) Finished aligning to item in 2 rounds -4.0 9.3 unbinned pixels; 39.6 0.2 nm along two shift axes; -35.5 -17.4 nm on specimen axes Total_Shift = 20.312705 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 37.69, -22.61 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -5.9, 4.0 unbinned pixels, 0.13 nm/sec Measured defocus = -1.36 microns changed by -0.34 to target 34 frames are being saved to U:\krios\20190806\slot11_0911.tif 34 frames are being saved to U:\krios\20190806\slot11_0912.tif 34 frames are being saved to U:\krios\20190806\slot11_0913.tif Item 368: Stage: -803.77 607.42 70 Label: 366 368 Tick time from program start = 61338.875 Running script 17 at item 367 (-802.68, 605.05) Remaining time to fill dewars is 28800 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.18 -0.20 (2nd move error 0.03 0.08) Finished aligning to item in 1 rounds 1.7 -4.4 unbinned pixels; 18.3 0.5 nm along two shift axes; 16.7 7.5 nm on specimen axes Total_Shift = 9.403232 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 4.74, -1.60 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -9.8, 3.8 unbinned pixels, 0.19 nm/sec Measured defocus = -0.15 microns changed by -1.65 to target Tilt-induced shift is 47.38, -30.07 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -1.2, 2.9 unbinned pixels, 0.06 nm/sec Measured defocus = -1.73 microns changed by -0.07 to target 34 frames are being saved to U:\krios\20190806\slot11_0914.tif 34 frames are being saved to U:\krios\20190806\slot11_0915.tif 34 frames are being saved to U:\krios\20190806\slot11_0916.tif Item 369: Stage: -802.68 605.05 70 Label: 367 369 Tick time from program start = 61461.055 Running script 17 at item 368 (-801.69, 602.66) Remaining time to fill dewars is 28680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 80 20 30, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.25 -0.14 (2nd move error 0.10 0.13) Finished aligning to item in 1 rounds Trimming A by 20 70 20 50, reference by 20 80 20 30 2.1 5.7 unbinned pixels; 18.2 16.1 nm along two shift axes; -22.6 7.0 nm on specimen axes Total_Shift = 12.149126 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 44.70, -24.88 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -11.6, 7.5 unbinned pixels, 0.25 nm/sec Measured defocus = -1.58 microns changed by -0.32 to target 34 frames are being saved to U:\krios\20190806\slot11_0917.tif 34 frames are being saved to U:\krios\20190806\slot11_0918.tif 34 frames are being saved to U:\krios\20190806\slot11_0919.tif Item 370: Stage: -801.69 602.66 70 Label: 368 370 Tick time from program start = 61573.828 Running script 17 at item 369 (-803.90, 601.59) Remaining time to fill dewars is 28440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 115 20 80, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.19 -0.21 (2nd move error 0.04 0.06) Finished aligning to item in 1 rounds Trimming A by 21 115 60 75, reference by 23 115 20 80 -0.0 -3.2 unbinned pixels; 11.8 4.9 nm along two shift axes; 12.6 0.6 nm on specimen axes Total_Shift = 6.454347 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 7.47, -4.40 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -9.1, 7.6 unbinned pixels, 0.21 nm/sec Measured defocus = -0.27 microns changed by -1.73 to target Tilt-induced shift is 51.60, -33.06 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -0.8, 5.0 unbinned pixels, 0.09 nm/sec Measured defocus = -1.89 microns changed by -0.11 to target 34 frames are being saved to U:\krios\20190806\slot11_0920.tif 34 frames are being saved to U:\krios\20190806\slot11_0921.tif 34 frames are being saved to U:\krios\20190806\slot11_0922.tif Item 371: Stage: -803.90 601.59 70 Label: 369 371 Tick time from program start = 61695.852 Running script 17 at item 370 (-804.98, 604.01) Remaining time to fill dewars is 28380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 65 20 60, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.23 -0.23 (2nd move error 0.08 0.03) Finished aligning to item in 1 rounds Trimming A by 20 60 20 65, reference by 20 65 20 60 -1.2 -2.2 unbinned pixels; 6.4 7.5 nm along two shift axes; 8.7 -4.2 nm on specimen axes Total_Shift = 4.935416 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 49.07, -29.63 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -6.9, 8.5 unbinned pixels, 0.19 nm/sec Measured defocus = -1.77 microns changed by -0.33 to target 34 frames are being saved to U:\krios\20190806\slot11_0923.tif 34 frames are being saved to U:\krios\20190806\slot11_0924.tif 34 frames are being saved to U:\krios\20190806\slot11_0925.tif Item 372: Stage: -804.98 604.01 70 Label: 370 372 Tick time from program start = 61808.516 Running script 17 at item 371 (-806.05, 606.33) Remaining time to fill dewars is 28380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 25 20 50, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.13 -0.26 (2nd move error 0.00 0.01) Finished aligning to item in 1 rounds Trimming A by 21 25 20 50, reference by 21 25 20 50 -0.9 -0.1 unbinned pixels; 0.9 3.5 nm along two shift axes; 0.6 -3.6 nm on specimen axes Total_Shift = 1.851791 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 16.04, -10.14 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -7.4, 5.8 unbinned pixels, 0.17 nm/sec Measured defocus = -0.59 microns changed by -1.61 to target Tilt-induced shift is 57.19, -35.91 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -1.1, 1.7 unbinned pixels, 0.04 nm/sec Measured defocus = -2.09 microns changed by -0.11 to target 34 frames are being saved to U:\krios\20190806\slot11_0926.tif 34 frames are being saved to U:\krios\20190806\slot11_0927.tif 34 frames are being saved to U:\krios\20190806\slot11_0928.tif Item 373: Stage: -806.05 606.33 70 Label: 371 373 Tick time from program start = 61928.466 Running script 17 at item 372 (-807.13, 608.70) Remaining time to fill dewars is 28260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 40, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.02 -0.25 (2nd move error -0.13 0.01) Finished aligning to item in 1 rounds Trimming A by 20 20 20 40, reference by 20 20 20 40 0.7 25.7 unbinned pixels; 93.4 41.2 nm along two shift axes; -100.0 -2.6 nm on specimen axes Total_Shift = 51.330021 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 52.51, -36.36 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.1, 6.2 unbinned pixels, 0.15 nm/sec Measured defocus = -1.97 microns changed by -0.33 to target 34 frames are being saved to U:\krios\20190806\slot11_0929.tif 34 frames are being saved to U:\krios\20190806\slot11_0930.tif 34 frames are being saved to U:\krios\20190806\slot11_0931.tif Item 374: Stage: -807.13 608.70 70 Label: 372 374 Tick time from program start = 62044.951 Running script 17 at item 373 (-808.36, 611.05) Remaining time to fill dewars is 28140 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 100 20 90 Trimming A by 20 20 20 20, reference by 20 50 20 20 Disparity in stage position after aligning to center of map frame: 0.17 -0.26 Trimming A by 20 20 20 55, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.28 -0.28 (2nd move error 0.11 -0.04) Finished aligning to item in 2 rounds Trimming A by 20 20 20 55, reference by 20 20 20 55 -4.8 -6.3 unbinned pixels; 17.1 26.9 nm along two shift axes; 25.7 -17.4 nm on specimen axes Total_Shift = 15.923794 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 17.48, -14.71 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was 0.1, 13.8 unbinned pixels, 0.24 nm/sec Measured defocus = -0.70 microns changed by -1.70 to target Tilt-induced shift is 62.93, -39.43 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was 2.4, 5.1 unbinned pixels, 0.10 nm/sec Measured defocus = -2.30 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_0932.tif 34 frames are being saved to U:\krios\20190806\slot11_0933.tif 34 frames are being saved to U:\krios\20190806\slot11_0934.tif Item 375: Stage: -808.36 611.05 70 Label: 373 375 Tick time from program start = 62173.949 Running script 17 at item 374 (-809.40, 613.31) Remaining time to fill dewars is 28020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 75, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.19 -0.28 (2nd move error 0.02 -0.04) Finished aligning to item in 1 rounds Trimming A by 20 20 20 75, reference by 20 20 20 75 -6.3 -2.5 unbinned pixels; 1.1 26.5 nm along two shift axes; 11.2 -24.0 nm on specimen axes Total_Shift = 13.582836 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 51.85, -34.30 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was 0.2, 9.6 unbinned pixels, 0.17 nm/sec Measured defocus = -1.92 microns changed by -0.58 to target 34 frames are being saved to U:\krios\20190806\slot11_0935.tif 34 frames are being saved to U:\krios\20190806\slot11_0936.tif 34 frames are being saved to U:\krios\20190806\slot11_0937.tif Item 376: Stage: -809.40 613.31 70 Label: 374 376 Tick time from program start = 62286.379 Running script 17 at item 375 (-810.53, 615.62) Remaining time to fill dewars is 27960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 100, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.15 -0.29 (2nd move error -0.03 -0.05) Finished aligning to item in 1 rounds Trimming A by 20 20 20 100, reference by 20 20 20 100 -6.6 6.3 unbinned pixels; 31.7 14.2 nm along two shift axes; -23.1 -26.9 nm on specimen axes Total_Shift = 18.202918 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -17.99, 14.44 unbinned pixels, unfiltered CCC is 0.0044 The average drift between shots was -5.8, -0.7 unbinned pixels, 0.10 nm/sec Measured defocus = 0.71 microns changed by -2.01 to target Tilt-induced shift is 34.45, -21.29 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was 1.1, 1.6 unbinned pixels, 0.03 nm/sec Measured defocus = -1.25 microns changed by -0.05 to target 34 frames are being saved to U:\krios\20190806\slot11_0938.tif 34 frames are being saved to U:\krios\20190806\slot11_0939.tif 34 frames are being saved to U:\krios\20190806\slot11_0940.tif Item 377: Stage: -810.53 615.62 70 Label: 375 377 Tick time from program start = 62409.089 Running script 17 at item 376 (-811.60, 618.02) Remaining time to fill dewars is 27840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 125, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.09 -0.29 (2nd move error -0.08 -0.05) Finished aligning to item in 1 rounds Trimming A by 20 20 20 125, reference by 20 20 20 125 -5.9 15.5 unbinned pixels; 64.5 2.0 nm along two shift axes; -58.9 -26.2 nm on specimen axes Total_Shift = 33.08763 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 21.58, -14.43 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -8.2, 8.0 unbinned pixels, 0.20 nm/sec Measured defocus = -0.80 microns changed by -0.50 to target 34 frames are being saved to U:\krios\20190806\slot11_0941.tif 34 frames are being saved to U:\krios\20190806\slot11_0942.tif 34 frames are being saved to U:\krios\20190806\slot11_0943.tif Item 378: Stage: -811.60 618.02 70 Label: 376 378 Tick time from program start = 62522.049 Running script 17 at item 377 (-811.62, 612.24) Remaining time to fill dewars is 27720 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 150, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.21 -0.33 (2nd move error 0.04 -0.10) Finished aligning to item in 1 rounds Trimming A by 20 20 20 150, reference by 20 20 20 150 -12.7 -2.9 unbinned pixels; 6.1 50.2 nm along two shift axes; 13.9 -48.9 nm on specimen axes Total_Shift = 26.1097 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -17.79, 10.55 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -2.2, 8.4 unbinned pixels, 0.15 nm/sec Measured defocus = 0.64 microns changed by -2.04 to target Tilt-induced shift is 36.19, -23.56 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was 1.9, 4.4 unbinned pixels, 0.08 nm/sec Measured defocus = -1.33 microns changed by -0.07 to target 34 frames are being saved to U:\krios\20190806\slot11_0944.tif 34 frames are being saved to U:\krios\20190806\slot11_0945.tif 34 frames are being saved to U:\krios\20190806\slot11_0946.tif Item 379: Stage: -811.62 612.24 70 Label: 377 379 Tick time from program start = 62644.354 Running script 17 at item 378 (-810.60, 610.02) Remaining time to fill dewars is 27660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 21 21 20 135, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.27 -0.29 (2nd move error 0.10 -0.05) Finished aligning to item in 1 rounds Trimming A by 20 20 20 130, reference by 21 21 20 135 -5.6 -1.3 unbinned pixels; 2.4 22.0 nm along two shift axes; 6.4 -21.4 nm on specimen axes Total_Shift = 11.443993 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 24.74, -17.07 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was 0.2, 11.5 unbinned pixels, 0.21 nm/sec Measured defocus = -0.93 microns changed by -0.57 to target 34 frames are being saved to U:\krios\20190806\slot11_0947.tif 34 frames are being saved to U:\krios\20190806\slot11_0948.tif 34 frames are being saved to U:\krios\20190806\slot11_0949.tif Item 380: Stage: -810.60 610.02 70 Label: 378 380 Tick time from program start = 62757.112 Running script 17 at item 379 (-809.39, 607.61) Remaining time to fill dewars is 27540 Dewars are NOT busy filling Trimming A by 20 100 20 95, reference by 20 20 20 20 Trimming A by 20 100 20 95, reference by 20 20 20 20 Trimming A by 20 100 20 95, reference by 24 100 20 90 Trimming A by 20 100 20 95, reference by 20 50 20 20 Disparity in stage position after aligning to center of map frame: 0.12 -0.20 Trimming A by 22 22 20 125, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.06 -0.31 (2nd move error -0.07 -0.13) Finished aligning to item in 2 rounds Trimming A by 22 22 20 125, reference by 22 22 20 125 -16.2 13.4 unbinned pixels; 70.2 38.2 nm along two shift axes; -48.5 -65.8 nm on specimen axes Total_Shift = 41.97941 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is -5.91, 1.41 unbinned pixels, unfiltered CCC is -0.0012 The average drift between shots was -5.9, 7.8 unbinned pixels, 0.17 nm/sec Measured defocus = 0.18 microns changed by -1.78 to target Tilt-induced shift is 40.21, -25.63 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was 1.3, 0.6 unbinned pixels, 0.03 nm/sec Measured defocus = -1.47 microns changed by -0.13 to target 34 frames are being saved to U:\krios\20190806\slot11_0950.tif 34 frames are being saved to U:\krios\20190806\slot11_0951.tif 34 frames are being saved to U:\krios\20190806\slot11_0952.tif Item 381: Stage: -809.39 607.61 70 Label: 379 381 Tick time from program start = 62889.011 Running script 17 at item 380 (-808.30, 605.23) Remaining time to fill dewars is 27540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 47 20 130, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.20 -0.27 (2nd move error 0.08 -0.09) Finished aligning to item in 1 rounds Trimming A by 22 51 20 125, reference by 20 47 20 130 -8.8 -2.9 unbinned pixels; 0.8 35.9 nm along two shift axes; 13.1 -33.5 nm on specimen axes Total_Shift = 18.481337 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 32.43, -22.18 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -1.7, 10.7 unbinned pixels, 0.19 nm/sec Measured defocus = -1.21 microns changed by -0.49 to target 34 frames are being saved to U:\krios\20190806\slot11_0953.tif 34 frames are being saved to U:\krios\20190806\slot11_0954.tif 34 frames are being saved to U:\krios\20190806\slot11_0955.tif Item 382: Stage: -808.30 605.23 70 Label: 380 382 Tick time from program start = 63001.674 Running script 17 at item 381 (-807.22, 602.85) Remaining time to fill dewars is 27300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 110 20 110, reference by 24 100 20 90 Disparity in stage position after aligning to target position: 0.16 -0.24 (2nd move error 0.04 -0.06) Finished aligning to item in 1 rounds Trimming A by 20 110 20 110, reference by 20 110 20 110 -7.7 -1.9 unbinned pixels; 3.0 30.4 nm along two shift axes; 9.0 -29.4 nm on specimen axes Total_Shift = 15.778851 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is -0.51, -0.05 unbinned pixels, unfiltered CCC is 0.0041 The average drift between shots was -4.5, 8.1 unbinned pixels, 0.17 nm/sec Measured defocus = 0.01 microns changed by -1.81 to target Tilt-induced shift is 46.63, -29.89 unbinned pixels, unfiltered CCC is 0.0040 The average drift between shots was 1.7, 4.8 unbinned pixels, 0.09 nm/sec Measured defocus = -1.71 microns changed by -0.09 to target 34 frames are being saved to U:\krios\20190806\slot11_0956.tif 34 frames are being saved to U:\krios\20190806\slot11_0957.tif 34 frames are being saved to U:\krios\20190806\slot11_0958.tif Item 383: Stage: -807.22 602.85 70 Label: 381 383 Tick time from program start = 63123.979 Running script 17 at item 382 (-191.30, 494.23) Remaining time to fill dewars is 27240 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 75 55 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.25 Disparity in stage position after aligning to target position: -0.09 -0.16 (2nd move error 0.10 0.07) Finished aligning to item in 2 rounds 0.4 3.7 unbinned pixels; 13.2 6.9 nm along two shift axes; -14.5 0.6 nm on specimen axes Total_Shift = 7.445978 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 67.61, -43.21 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -0.4, 9.0 unbinned pixels, 0.16 nm/sec Measured defocus = -2.48 microns changed by 0.58 to target 34 frames are being saved to U:\krios\20190806\slot11_0959.tif 34 frames are being saved to U:\krios\20190806\slot11_0960.tif 34 frames are being saved to U:\krios\20190806\slot11_0961.tif Item 384: Stage: -191.30 494.23 80 Label: 382 384 Tick time from program start = 63256.455 Running script 17 at item 383 (-190.27, 491.90) Remaining time to fill dewars is 27120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.10 -0.19 (2nd move error 0.09 0.04) Finished aligning to item in 1 rounds -0.3 -5.0 unbinned pixels; 18.1 8.7 nm along two shift axes; 19.6 -0.1 nm on specimen axes Total_Shift = 10.057793 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 34.57, -22.29 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -5.1, 9.6 unbinned pixels, 0.19 nm/sec Measured defocus = -1.27 microns changed by -0.73 to target 34 frames are being saved to U:\krios\20190806\slot11_0962.tif 34 frames are being saved to U:\krios\20190806\slot11_0963.tif 34 frames are being saved to U:\krios\20190806\slot11_0964.tif Item 385: Stage: -190.27 491.90 80 Label: 383 385 Tick time from program start = 63369.338 Running script 17 at item 384 (-185.83, 482.74) Remaining time to fill dewars is 27000 Dewars are NOT busy filling Trimming A by 24 80 60 20, reference by 20 20 20 20 Trimming A by 24 80 60 20, reference by 20 20 20 20 Trimming A by 24 80 60 20, reference by 20 20 20 20 Trimming A by 24 80 60 20, reference by 24 75 55 20 Disparity in stage position after aligning to center of map frame: -0.13 -0.23 Trimming A by 20 20 20 20, reference by 24 75 55 20 Disparity in stage position after aligning to target position: -0.14 -0.33 (2nd move error -0.01 -0.12) Finished aligning to item in 2 rounds -15.1 4.1 unbinned pixels; 34.8 48.3 nm along two shift axes; -12.7 -59.7 nm on specimen axes Total_Shift = 31.342427 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 31.33, -19.00 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -2.6, 5.7 unbinned pixels, 0.11 nm/sec Measured defocus = -1.13 microns changed by -0.97 to target 34 frames are being saved to U:\krios\20190806\slot11_0965.tif 34 frames are being saved to U:\krios\20190806\slot11_0966.tif 34 frames are being saved to U:\krios\20190806\slot11_0967.tif Item 386: Stage: -185.83 482.74 80 Label: 384 386 Tick time from program start = 63491.564 Running script 17 at item 385 (-184.76, 480.39) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 90 45 20, reference by 24 75 55 20 Disparity in stage position after aligning to target position: -0.12 -0.32 (2nd move error 0.01 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 90 50 20, reference by 20 90 45 20 -14.8 -0.1 unbinned pixels; 18.9 53.5 nm along two shift axes; 3.6 -57.6 nm on specimen axes Total_Shift = 29.643798 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 32.70, -19.60 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -4.0, 7.6 unbinned pixels, 0.15 nm/sec Measured defocus = -1.18 microns changed by -1.02 to target 34 frames are being saved to U:\krios\20190806\slot11_0968.tif 34 frames are being saved to U:\krios\20190806\slot11_0969.tif 34 frames are being saved to U:\krios\20190806\slot11_0970.tif Item 387: Stage: -184.76 480.39 80 Label: 385 387 Tick time from program start = 63604.181 Running script 17 at item 386 (-187.01, 479.34) Remaining time to fill dewars is 26880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 110 20 20, reference by 24 75 55 20 Disparity in stage position after aligning to target position: -0.10 -0.38 (2nd move error 0.04 -0.16) Finished aligning to item in 1 rounds Trimming A by 20 110 20 20, reference by 20 110 20 20 -22.6 -3.1 unbinned pixels; 18.0 86.0 nm along two shift axes; 16.9 -87.2 nm on specimen axes Total_Shift = 45.607487 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 28.94, -18.69 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -1.0, 11.4 unbinned pixels, 0.20 nm/sec Measured defocus = -1.06 microns changed by -1.24 to target 34 frames are being saved to U:\krios\20190806\slot11_0971.tif 34 frames are being saved to U:\krios\20190806\slot11_0972.tif 34 frames are being saved to U:\krios\20190806\slot11_0973.tif Item 388: Stage: -187.01 479.34 80 Label: 386 388 Tick time from program start = 63717.438 Running script 17 at item 387 (-188.11, 481.46) Remaining time to fill dewars is 26700 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 75 55 20 Disparity in stage position after aligning to center of map frame: -0.22 -0.25 Trimming A by 20 40 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.13 -0.12 (2nd move error 0.09 0.11) Finished aligning to item in 2 rounds Trimming A by 20 40 20 20, reference by 20 40 20 20 0.9 0.3 unbinned pixels; 0.1 3.8 nm along two shift axes; -1.3 3.6 nm on specimen axes Total_Shift = 1.962695 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 71.26, -46.27 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -5.1, 14.4 unbinned pixels, 0.27 nm/sec Measured defocus = -2.63 microns changed by 0.23 to target 34 frames are being saved to U:\krios\20190806\slot11_0974.tif 34 frames are being saved to U:\krios\20190806\slot11_0975.tif 34 frames are being saved to U:\krios\20190806\slot11_0976.tif Item 389: Stage: -188.11 481.46 80 Label: 387 389 Tick time from program start = 63839.337 Running script 17 at item 388 (-189.15, 483.77) Remaining time to fill dewars is 26580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.05 -0.18 (2nd move error 0.17 0.05) Finished aligning to item in 1 rounds 1.0 -0.4 unbinned pixels; 2.6 3.0 nm along two shift axes; 1.2 3.9 nm on specimen axes Total_Shift = 2.082793 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 42.56, -24.83 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -7.8, 8.4 unbinned pixels, 0.21 nm/sec Measured defocus = -1.52 microns changed by -0.98 to target 34 frames are being saved to U:\krios\20190806\slot11_0977.tif 34 frames are being saved to U:\krios\20190806\slot11_0978.tif 34 frames are being saved to U:\krios\20190806\slot11_0979.tif Item 390: Stage: -189.15 483.77 80 Label: 388 390 Tick time from program start = 63955.090 Running script 17 at item 389 (-195.66, 497.79) Remaining time to fill dewars is 26520 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.25 -0.22 (2nd move error -0.06 0.01) Finished aligning to item in 1 rounds -4.6 14.5 unbinned pixels; 59.3 5.2 nm along two shift axes; -55.4 -21.0 nm on specimen axes Total_Shift = 30.411266 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 49.22, -31.37 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -2.8, 2.3 unbinned pixels, 0.06 nm/sec Measured defocus = -1.80 microns changed by 0.50 to target 34 frames are being saved to U:\krios\20190806\slot11_0980.tif 34 frames are being saved to U:\krios\20190806\slot11_0981.tif 34 frames are being saved to U:\krios\20190806\slot11_0982.tif Item 391: Stage: -195.66 497.79 80 Label: 389 391 Tick time from program start = 64071.763 Running script 17 at item 390 (-197.96, 502.60) Remaining time to fill dewars is 26520 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 100 20 20 45 Trimming A by 20 20 20 20, reference by 80 20 20 45 Trimming A by 20 20 20 20, reference by 105 22 70 20 Disparity in stage position after aligning to center of map frame: -0.17 -0.23 Disparity in stage position after aligning to target position: -0.09 -0.28 (2nd move error 0.08 -0.06) Finished aligning to item in 2 rounds -8.2 -0.0 unbinned pixels; 10.7 29.6 nm along two shift axes; 1.8 -32.0 nm on specimen axes Total_Shift = 16.448941 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 10.57, -4.61 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was 1.8, 5.2 unbinned pixels, 0.10 nm/sec Measured defocus = -0.35 microns changed by -1.05 to target 34 frames are being saved to U:\krios\20190806\slot11_0983.tif 34 frames are being saved to U:\krios\20190806\slot11_0984.tif 34 frames are being saved to U:\krios\20190806\slot11_0985.tif Item 392: Stage: -197.96 502.60 80 Label: 390 392 Tick time from program start = 64191.120 Running script 17 at item 391 (-208.72, 520.00) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Trimming A by 120 20 20 20, reference by 190 24 20 190 Trimming A by 120 20 20 20, reference by 100 20 20 45 Trimming A by 120 20 20 20, reference by 80 20 20 45 Trimming A by 120 20 20 20, reference by 20 20 20 100 Trimming A by 120 20 20 20, reference by 20 20 20 20 Trimming A by 120 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.26 Trimming A by 55 20 20 20, reference by 100 20 20 45 Disparity in stage position after aligning to target position: -0.22 -0.22 (2nd move error 0.05 0.01) Finished aligning to item in 2 rounds Trimming A by 55 20 20 20, reference by 55 20 20 20 1.8 -2.7 unbinned pixels; 12.3 2.5 nm along two shift axes; 10.1 7.6 nm on specimen axes Total_Shift = 6.520235 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 4.04, -1.09 unbinned pixels, unfiltered CCC is 0.0034 The average drift between shots was -5.5, 7.7 unbinned pixels, 0.17 nm/sec Measured defocus = -0.12 microns changed by -1.38 to target 34 frames are being saved to U:\krios\20190806\slot11_0986.tif 34 frames are being saved to U:\krios\20190806\slot11_0987.tif 34 frames are being saved to U:\krios\20190806\slot11_0988.tif Item 393: Stage: -208.72 520.00 80 Label: 391 393 Tick time from program start = 64314.220 Running script 17 at item 392 (-201.19, 503.83) Remaining time to fill dewars is 26280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.00 -0.30 (2nd move error 0.17 -0.09) Finished aligning to item in 1 rounds -9.4 1.4 unbinned pixels; 17.7 31.8 nm along two shift axes; -3.7 -37.0 nm on specimen axes Total_Shift = 19.099503 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 17.57, -11.87 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was 2.0, 5.8 unbinned pixels, 0.11 nm/sec Measured defocus = -0.65 microns changed by -0.95 to target 34 frames are being saved to U:\krios\20190806\slot11_0989.tif 34 frames are being saved to U:\krios\20190806\slot11_0990.tif 34 frames are being saved to U:\krios\20190806\slot11_0991.tif Item 394: Stage: -201.19 503.83 80 Label: 392 394 Tick time from program start = 64427.929 Running script 17 at item 393 (-198.95, 499.13) Remaining time to fill dewars is 26100 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 75 55 20 Disparity in stage position after aligning to center of map frame: -0.19 -0.19 Disparity in stage position after aligning to target position: -0.28 -0.28 (2nd move error -0.10 -0.11) Finished aligning to item in 2 rounds -14.0 19.5 unbinned pixels; 90.1 20.8 nm along two shift axes; -73.1 -58.4 nm on specimen axes Total_Shift = 48.027011 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 27.13, -15.18 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -2.6, 1.8 unbinned pixels, 0.06 nm/sec Measured defocus = -0.96 microns changed by -0.74 to target 34 frames are being saved to U:\krios\20190806\slot11_0992.tif 34 frames are being saved to U:\krios\20190806\slot11_0993.tif 34 frames are being saved to U:\krios\20190806\slot11_0994.tif Item 395: Stage: -198.95 499.13 80 Label: 393 395 Tick time from program start = 64549.844 Running script 17 at item 394 (-190.31, 480.40) Remaining time to fill dewars is 25980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 60 30 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.15 -0.20 (2nd move error 0.07 0.04) Finished aligning to item in 1 rounds Trimming A by 20 60 20 20, reference by 20 60 30 20 -0.8 -3.8 unbinned pixels; 13.0 8.6 nm along two shift axes; 15.0 -2.3 nm on specimen axes Total_Shift = 7.776322 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 14.90, -6.99 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -4.9, 7.1 unbinned pixels, 0.15 nm/sec Measured defocus = -0.51 microns changed by -1.29 to target 34 frames are being saved to U:\krios\20190806\slot11_0995.tif 34 frames are being saved to U:\krios\20190806\slot11_0996.tif 34 frames are being saved to U:\krios\20190806\slot11_0997.tif Item 396: Stage: -190.31 480.40 80 Label: 394 396 Tick time from program start = 64663.194 Running script 17 at item 395 (-192.54, 479.31) Remaining time to fill dewars is 25860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 89 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.09 -0.27 (2nd move error 0.12 -0.04) Finished aligning to item in 1 rounds Trimming A by 20 90 20 20, reference by 20 89 20 20 -4.0 -2.4 unbinned pixels; 3.8 18.0 nm along two shift axes; 10.4 -15.0 nm on specimen axes Total_Shift = 9.350147 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 18.69, -12.40 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -2.3, 9.7 unbinned pixels, 0.18 nm/sec Measured defocus = -0.69 microns changed by -1.21 to target 34 frames are being saved to U:\krios\20190806\slot11_0998.tif 34 frames are being saved to U:\krios\20190806\slot11_0999.tif 34 frames are being saved to U:\krios\20190806\slot11_1000.tif Item 397: Stage: -192.54 479.31 80 Label: 395 397 Tick time from program start = 64776.482 Running script 17 at item 396 (-193.60, 481.65) Remaining time to fill dewars is 25860 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 75 55 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.28 Aligning to target with image shift equivalent to stage shift: 0.09 -0.09 Finished aligning to item in 1 rounds -10.6 -4.4 unbinned pixels; 2.2 44.8 nm along two shift axes; 19.2 -40.4 nm on specimen axes Total_Shift = 22.978867 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 26.07, -16.44 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -0.8, 7.8 unbinned pixels, 0.14 nm/sec Measured defocus = -0.95 microns changed by -1.05 to target 34 frames are being saved to U:\krios\20190806\slot11_1001.tif 34 frames are being saved to U:\krios\20190806\slot11_1002.tif 34 frames are being saved to U:\krios\20190806\slot11_1003.tif Item 398: Stage: -193.60 481.65 80 Label: 396 398 Tick time from program start = 64889.146 Running script 17 at item 397 (-200.19, 495.67) Remaining time to fill dewars is 25680 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.24 -0.33 (2nd move error -0.06 -0.15) Finished aligning to item in 1 rounds -21.2 13.6 unbinned pixels; 77.9 55.9 nm along two shift axes; -48.6 -85.5 nm on specimen axes Total_Shift = 50.504443 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 37.60, -22.54 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -2.7, 3.1 unbinned pixels, 0.07 nm/sec Measured defocus = -1.36 microns changed by -0.74 to target 34 frames are being saved to U:\krios\20190806\slot11_1004.tif 34 frames are being saved to U:\krios\20190806\slot11_1005.tif 34 frames are being saved to U:\krios\20190806\slot11_1006.tif Item 399: Stage: -200.19 495.67 80 Label: 397 399 Tick time from program start = 65018.284 Running script 17 at item 398 (-201.21, 498.09) Remaining time to fill dewars is 25560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.15 -0.34 (2nd move error 0.04 -0.16) Finished aligning to item in 1 rounds -18.8 -1.4 unbinned pixels; 19.4 69.6 nm along two shift axes; 9.3 -72.7 nm on specimen axes Total_Shift = 37.608526 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 37.77, -25.75 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was 2.6, 8.6 unbinned pixels, 0.16 nm/sec Measured defocus = -1.41 microns changed by -0.79 to target 34 frames are being saved to U:\krios\20190806\slot11_1007.tif 34 frames are being saved to U:\krios\20190806\slot11_1008.tif 34 frames are being saved to U:\krios\20190806\slot11_1009.tif Item 400: Stage: -201.21 498.09 80 Label: 398 400 Tick time from program start = 65131.150 Running script 17 at item 399 (-206.70, 503.87) Remaining time to fill dewars is 25440 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 190 24 20 190 Trimming A by 20 20 20 20, reference by 100 20 20 45 Trimming A by 20 20 20 20, reference by 80 20 20 45 Trimming A by 20 20 20 20, reference by 20 20 20 100 Trimming A by 20 20 20 20, reference by 20 20 20 105 Disparity in stage position after aligning to center of map frame: -0.19 -0.26 Disparity in stage position after aligning to target position: -0.24 -0.23 (2nd move error -0.05 0.01) Finished aligning to item in 2 rounds 1.2 10.4 unbinned pixels; 36.8 20.0 nm along two shift axes; -40.8 2.5 nm on specimen axes Total_Shift = 20.988542 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 37.60, -24.76 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -7.5, 4.6 unbinned pixels, 0.16 nm/sec Measured defocus = -1.39 microns changed by -0.91 to target 34 frames are being saved to U:\krios\20190806\slot11_1010.tif 34 frames are being saved to U:\krios\20190806\slot11_1011.tif 34 frames are being saved to U:\krios\20190806\slot11_1012.tif Item 401: Stage: -206.70 503.87 80 Label: 399 401 Tick time from program start = 65253.845 Running script 17 at item 400 (-201.24, 492.10) Remaining time to fill dewars is 25260 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 20 20 100 Trimming A by 20 20 20 20, reference by 20 20 20 105 Trimming A by 20 20 20 20, reference by 23 105 20 185 Disparity in stage position after aligning to center of map frame: -0.27 -0.26 Disparity in stage position after aligning to target position: -0.01 -0.14 (2nd move error 0.26 0.09) Finished aligning to item in 2 rounds 1.4 4.8 unbinned pixels; 15.7 12.3 nm along two shift axes; -18.9 4.6 nm on specimen axes Total_Shift = 9.987615 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 40.80, -26.21 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -6.9, 7.3 unbinned pixels, 0.18 nm/sec Measured defocus = -1.50 microns changed by -0.90 to target 34 frames are being saved to U:\krios\20190806\slot11_1013.tif 34 frames are being saved to U:\krios\20190806\slot11_1014.tif 34 frames are being saved to U:\krios\20190806\slot11_1015.tif Item 402: Stage: -201.24 492.10 80 Label: 400 402 Tick time from program start = 65376.961 Running script 17 at item 401 (-196.94, 482.93) Remaining time to fill dewars is 25260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.17 -0.32 (2nd move error -0.04 -0.05) Finished aligning to item in 1 rounds -6.2 8.2 unbinned pixels; 38.5 10.1 nm along two shift axes; -30.8 -26.0 nm on specimen axes Total_Shift = 20.68156 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 73.12, -49.17 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was -2.9, 9.1 unbinned pixels, 0.17 nm/sec Measured defocus = -2.72 microns changed by 0.22 to target 34 frames are being saved to U:\krios\20190806\slot11_1016.tif 34 frames are being saved to U:\krios\20190806\slot11_1017.tif 34 frames are being saved to U:\krios\20190806\slot11_1018.tif Item 403: Stage: -196.94 482.93 80 Label: 401 403 Tick time from program start = 65489.641 Running script 17 at item 402 (-195.82, 480.61) Remaining time to fill dewars is 25080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 40 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.13 -0.29 (2nd move error 0.00 -0.03) Finished aligning to item in 1 rounds Trimming A by 20 40 20 20, reference by 20 40 20 20 -5.1 -0.1 unbinned pixels; 6.1 18.6 nm along two shift axes; 1.7 -19.8 nm on specimen axes Total_Shift = 10.200995 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 6.60, -0.39 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -7.8, 7.5 unbinned pixels, 0.19 nm/sec Measured defocus = -0.18 microns changed by -1.12 to target 34 frames are being saved to U:\krios\20190806\slot11_1019.tif 34 frames are being saved to U:\krios\20190806\slot11_1020.tif 34 frames are being saved to U:\krios\20190806\slot11_1021.tif Item 404: Stage: -195.82 480.61 80 Label: 402 404 Tick time from program start = 65601.665 Running script 17 at item 403 (-198.10, 479.53) Remaining time to fill dewars is 24960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 65 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.06 -0.36 (2nd move error 0.07 -0.11) Finished aligning to item in 1 rounds Trimming A by 20 70 20 20, reference by 20 65 20 20 -14.1 -1.4 unbinned pixels; 13.4 52.7 nm along two shift axes; 8.3 -54.5 nm on specimen axes Total_Shift = 28.282085 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -2.06, 1.53 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -3.0, 8.8 unbinned pixels, 0.17 nm/sec Measured defocus = 0.08 microns changed by -1.38 to target 34 frames are being saved to U:\krios\20190806\slot11_1022.tif 34 frames are being saved to U:\krios\20190806\slot11_1023.tif 34 frames are being saved to U:\krios\20190806\slot11_1024.tif Item 405: Stage: -198.10 479.53 80 Label: 403 405 Tick time from program start = 65714.532 Running script 17 at item 404 (-199.17, 481.87) Remaining time to fill dewars is 24780 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 24 75 55 20 Disparity in stage position after aligning to center of map frame: -0.12 -0.21 Disparity in stage position after aligning to target position: -0.17 -0.37 (2nd move error -0.06 -0.18) Finished aligning to item in 2 rounds -22.9 12.8 unbinned pixels; 76.9 63.2 nm along two shift axes; -44.9 -91.8 nm on specimen axes Total_Shift = 52.473679 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 0.81, -0.44 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -4.7, 5.6 unbinned pixels, 0.13 nm/sec Measured defocus = -0.03 microns changed by -1.37 to target 34 frames are being saved to U:\krios\20190806\slot11_1025.tif 34 frames are being saved to U:\krios\20190806\slot11_1026.tif 34 frames are being saved to U:\krios\20190806\slot11_1027.tif Item 406: Stage: -199.17 481.87 80 Label: 404 406 Tick time from program start = 65836.790 Running script 17 at item 405 (-200.25, 484.25) Remaining time to fill dewars is 24660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.20 -0.39 (2nd move error -0.08 -0.20) Finished aligning to item in 1 rounds -25.9 16.3 unbinned pixels; 93.6 68.6 nm along two shift axes; -57.9 -104.1 nm on specimen axes Total_Shift = 61.124159 -4.2 10.3 unbinned pixels; 43.2 0.3 nm along two shift axes; -39.0 -18.5 nm on specimen axes Total_Shift = 22.16164 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 4.10, -2.91 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -2.5, 4.2 unbinned pixels, 0.09 nm/sec Measured defocus = -0.15 microns changed by -1.35 to target 34 frames are being saved to U:\krios\20190806\slot11_1028.tif 34 frames are being saved to U:\krios\20190806\slot11_1029.tif 34 frames are being saved to U:\krios\20190806\slot11_1030.tif Item 407: Stage: -200.25 484.25 80 Label: 405 407 Tick time from program start = 65954.914 Running script 17 at item 406 (-205.59, 495.71) Remaining time to fill dewars is 24540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.30 -0.25 (2nd move error -0.04 -0.01) Finished aligning to item in 1 rounds -1.2 7.9 unbinned pixels; 30.6 7.5 nm along two shift axes; -30.5 -6.4 nm on specimen axes Total_Shift = 15.975669 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 15.66, -10.75 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -6.2, 4.5 unbinned pixels, 0.14 nm/sec Measured defocus = -0.59 microns changed by -1.01 to target 34 frames are being saved to U:\krios\20190806\slot11_1031.tif 34 frames are being saved to U:\krios\20190806\slot11_1032.tif 34 frames are being saved to U:\krios\20190806\slot11_1033.tif Item 408: Stage: -205.59 495.71 80 Label: 406 408 Tick time from program start = 66068.389 Running script 17 at item 407 (-217.81, 515.55) Remaining time to fill dewars is 24360 Dewars are NOT busy filling Trimming A by 200 23 20 190, reference by 190 24 20 190 Trimming A by 200 23 20 190, reference by 100 20 20 45 Trimming A by 200 23 20 190, reference by 20 20 20 100 Trimming A by 200 23 20 190, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.13 Trimming A by 35 23 20 70, reference by 190 24 20 190 Disparity in stage position after aligning to target position: -0.09 -0.03 (2nd move error 0.17 0.09) Finished aligning to item in 2 rounds Trimming A by 31 21 20 70, reference by 35 23 20 70 0.4 1.5 unbinned pixels; 4.9 3.6 nm along two shift axes; -5.8 1.2 nm on specimen axes Total_Shift = 3.034875 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 3.04, 1.04 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -10.8, 9.1 unbinned pixels, 0.25 nm/sec Measured defocus = -0.06 microns changed by -1.64 to target Tilt-induced shift is 41.28, -26.25 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -2.3, 6.3 unbinned pixels, 0.12 nm/sec Measured defocus = -1.51 microns changed by -0.19 to target 34 frames are being saved to U:\krios\20190806\slot11_1034.tif 34 frames are being saved to U:\krios\20190806\slot11_1035.tif 34 frames are being saved to U:\krios\20190806\slot11_1036.tif Item 409: Stage: -217.81 515.55 80 Label: 407 409 Tick time from program start = 66201.115 Running script 17 at item 408 (-215.46, 510.80) Remaining time to fill dewars is 24240 Dewars are NOT busy filling Trimming A by 21 21 20 105, reference by 190 24 20 190 Trimming A by 21 21 20 105, reference by 100 20 20 45 Trimming A by 21 21 20 105, reference by 20 20 20 100 Trimming A by 21 21 20 105, reference by 20 20 20 20 Trimming A by 21 21 20 105, reference by 20 20 20 105 Trimming A by 21 21 20 105, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.17 Trimming A by 20 20 20 20, reference by 20 20 20 100 Disparity in stage position after aligning to target position: -0.12 -0.04 (2nd move error 0.07 0.12) Finished aligning to item in 2 rounds -1.7 2.4 unbinned pixels; 11.0 2.6 nm along two shift axes; -8.9 -7.1 nm on specimen axes Total_Shift = 5.870534 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 38.32, -27.33 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -0.5, 9.9 unbinned pixels, 0.18 nm/sec Measured defocus = -1.45 microns changed by -0.35 to target 34 frames are being saved to U:\krios\20190806\slot11_1037.tif 34 frames are being saved to U:\krios\20190806\slot11_1038.tif 34 frames are being saved to U:\krios\20190806\slot11_1039.tif Item 410: Stage: -215.46 510.80 80 Label: 408 410 Tick time from program start = 66321.735 Running script 17 at item 409 (-212.22, 504.05) Remaining time to fill dewars is 24060 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 190 24 20 190 Trimming A by 20 20 20 20, reference by 100 20 20 45 Trimming A by 20 20 20 20, reference by 80 20 20 45 Trimming A by 20 20 20 20, reference by 20 20 20 100 Trimming A by 20 20 20 20, reference by 20 20 20 105 Disparity in stage position after aligning to center of map frame: -0.13 -0.21 Disparity in stage position after aligning to target position: -0.19 -0.35 (2nd move error -0.06 -0.16) Finished aligning to item in 2 rounds -23.0 18.7 unbinned pixels; 98.8 54.6 nm along two shift axes; -67.9 -93.3 nm on specimen axes Total_Shift = 59.25557 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 18.00, -11.66 unbinned pixels, unfiltered CCC is 0.0002 The average drift between shots was -3.7, 3.2 unbinned pixels, 0.09 nm/sec Measured defocus = -0.66 microns changed by -1.24 to target 34 frames are being saved to U:\krios\20190806\slot11_1040.tif 34 frames are being saved to U:\krios\20190806\slot11_1041.tif 34 frames are being saved to U:\krios\20190806\slot11_1042.tif Item 411: Stage: -212.22 504.05 80 Label: 409 411 Tick time from program start = 66442.214 Running script 17 at item 410 (-201.34, 480.58) Remaining time to fill dewars is 23940 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 20 20 105 Trimming A by 20 20 20 20, reference by 23 105 20 185 Disparity in stage position after aligning to center of map frame: -0.20 -0.29 Trimming A by 20 30 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: 0.02 -0.12 (2nd move error 0.21 0.16) Finished aligning to item in 2 rounds Trimming A by 20 26 20 20, reference by 20 30 20 20 -0.6 1.3 unbinned pixels; 5.4 0.1 nm along two shift axes; -4.8 -2.4 nm on specimen axes Total_Shift = 2.759508 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 11.37, -5.62 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -6.7, 7.7 unbinned pixels, 0.18 nm/sec Measured defocus = -0.39 microns changed by -1.61 to target Tilt-induced shift is 51.84, -32.90 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -0.3, 4.3 unbinned pixels, 0.08 nm/sec Measured defocus = -1.90 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_1043.tif 34 frames are being saved to U:\krios\20190806\slot11_1044.tif 34 frames are being saved to U:\krios\20190806\slot11_1045.tif Item 412: Stage: -201.34 480.58 80 Label: 410 412 Tick time from program start = 66574.253 Running script 17 at item 411 (-203.60, 479.50) Remaining time to fill dewars is 23760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 60 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.06 -0.22 (2nd move error 0.14 0.04) Finished aligning to item in 1 rounds Trimming A by 20 55 20 20, reference by 20 60 20 20 0.4 0.4 unbinned pixels; 0.8 2.1 nm along two shift axes; -1.5 1.6 nm on specimen axes Total_Shift = 1.115182 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 51.25, -32.73 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -4.6, 8.8 unbinned pixels, 0.18 nm/sec Measured defocus = -1.88 microns changed by -0.22 to target 34 frames are being saved to U:\krios\20190806\slot11_1046.tif 34 frames are being saved to U:\krios\20190806\slot11_1047.tif 34 frames are being saved to U:\krios\20190806\slot11_1048.tif Item 413: Stage: -203.60 479.50 80 Label: 411 413 Tick time from program start = 66686.870 Running script 17 at item 412 (-204.65, 481.84) Remaining time to fill dewars is 23700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.02 -0.21 (2nd move error 0.19 0.07) Finished aligning to item in 1 rounds -1.3 -3.8 unbinned pixels; 12.5 10.3 nm along two shift axes; 15.2 -4.1 nm on specimen axes Total_Shift = 8.086535 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 17.15, -8.52 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -5.0, 6.7 unbinned pixels, 0.15 nm/sec Measured defocus = -0.59 microns changed by -1.61 to target Tilt-induced shift is 59.27, -37.50 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -0.5, 4.5 unbinned pixels, 0.08 nm/sec Measured defocus = -2.17 microns changed by -0.03 to target 34 frames are being saved to U:\krios\20190806\slot11_1049.tif 34 frames are being saved to U:\krios\20190806\slot11_1050.tif 34 frames are being saved to U:\krios\20190806\slot11_1051.tif Item 414: Stage: -204.65 481.84 80 Label: 412 414 Tick time from program start = 66810.080 Running script 17 at item 413 (-210.08, 493.58) Remaining time to fill dewars is 23460 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 20 20 100 Trimming A by 20 20 20 20, reference by 20 20 20 105 Trimming A by 20 20 20 20, reference by 23 105 20 185 Disparity in stage position after aligning to center of map frame: -0.19 -0.21 Disparity in stage position after aligning to target position: -0.25 -0.32 (2nd move error -0.07 -0.12) Finished aligning to item in 2 rounds -14.2 14.3 unbinned pixels; 71.2 29.5 nm along two shift axes; -52.8 -58.2 nm on specimen axes Total_Shift = 40.335145 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 61.47, -39.93 unbinned pixels, unfiltered CCC is 0.0016 The average drift between shots was -3.4, 7.4 unbinned pixels, 0.15 nm/sec Measured defocus = -2.26 microns changed by -0.04 to target 34 frames are being saved to U:\krios\20190806\slot11_1052.tif 34 frames are being saved to U:\krios\20190806\slot11_1053.tif 34 frames are being saved to U:\krios\20190806\slot11_1054.tif Item 415: Stage: -210.08 493.58 80 Label: 413 415 Tick time from program start = 66930.388 Running script 17 at item 414 (-217.69, 509.72) Remaining time to fill dewars is 23400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 20 20 100 Disparity in stage position after aligning to target position: -0.17 -0.12 (2nd move error 0.03 0.03) Finished aligning to item in 1 rounds -2.4 -1.6 unbinned pixels; 2.8 11.3 nm along two shift axes; 6.9 -9.2 nm on specimen axes Total_Shift = 5.877389 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 16.91, -14.72 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -1.6, 12.3 unbinned pixels, 0.22 nm/sec Measured defocus = -0.68 microns changed by -1.72 to target Tilt-induced shift is 64.82, -40.43 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -0.7, 4.7 unbinned pixels, 0.08 nm/sec Measured defocus = -2.36 microns changed by -0.04 to target 34 frames are being saved to U:\krios\20190806\slot11_1055.tif 34 frames are being saved to U:\krios\20190806\slot11_1056.tif 34 frames are being saved to U:\krios\20190806\slot11_1057.tif Item 416: Stage: -217.69 509.72 80 Label: 414 416 Tick time from program start = 67053.348 Running script 17 at item 415 (-218.78, 512.07) Remaining time to fill dewars is 23280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 25 20 20 60, reference by 20 20 20 100 Disparity in stage position after aligning to target position: -0.13 -0.13 (2nd move error 0.07 0.02) Finished aligning to item in 1 rounds Trimming A by 20 20 20 60, reference by 25 20 20 60 0.3 -0.9 unbinned pixels; 3.9 0.3 nm along two shift axes; 3.6 1.4 nm on specimen axes Total_Shift = 1.992223 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 54.90, -34.60 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -3.2, 7.6 unbinned pixels, 0.15 nm/sec Measured defocus = -2.01 microns changed by -0.49 to target 34 frames are being saved to U:\krios\20190806\slot11_1058.tif 34 frames are being saved to U:\krios\20190806\slot11_1059.tif 34 frames are being saved to U:\krios\20190806\slot11_1060.tif Item 417: Stage: -218.78 512.07 80 Label: 415 417 Tick time from program start = 67165.965 Running script 17 at item 416 (-220.02, 514.50) Remaining time to fill dewars is 23160 Dewars are NOT busy filling Trimming A by 200 24 20 190, reference by 190 24 20 190 Trimming A by 200 24 20 190, reference by 100 20 20 45 Trimming A by 200 24 20 190, reference by 20 20 20 100 Trimming A by 200 24 20 190, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.15 -0.13 Trimming A by 36 24 20 120, reference by 190 24 20 190 Disparity in stage position after aligning to target position: -0.01 -0.10 (2nd move error 0.14 0.01) Finished aligning to item in 2 rounds Trimming A by 32 22 20 120, reference by 36 24 20 120 1.3 -5.0 unbinned pixels; 20.0 2.9 nm along two shift axes; 19.1 6.0 nm on specimen axes Total_Shift = 10.274514 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -14.62, 8.57 unbinned pixels, unfiltered CCC is 0.0082 The average drift between shots was -5.6, 11.2 unbinned pixels, 0.22 nm/sec Measured defocus = 0.52 microns changed by -1.82 to target Tilt-induced shift is 32.62, -21.54 unbinned pixels, unfiltered CCC is 0.0050 The average drift between shots was 0.4, 5.1 unbinned pixels, 0.09 nm/sec Measured defocus = -1.21 microns changed by -0.09 to target 34 frames are being saved to U:\krios\20190806\slot11_1061.tif 34 frames are being saved to U:\krios\20190806\slot11_1062.tif 34 frames are being saved to U:\krios\20190806\slot11_1063.tif Item 418: Stage: -220.02 514.50 80 Label: 416 418 Tick time from program start = 67297.817 Running script 17 at item 417 (-219.95, 508.61) Remaining time to fill dewars is 22980 Dewars are NOT busy filling Trimming A by 20 20 20 105, reference by 190 24 20 190 Trimming A by 20 20 20 105, reference by 100 20 20 45 Trimming A by 20 20 20 105, reference by 20 20 20 100 Trimming A by 20 20 20 105, reference by 20 20 20 20 Trimming A by 20 20 20 105, reference by 20 20 20 105 Trimming A by 20 20 20 105, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.11 -0.18 Trimming A by 20 20 20 85, reference by 20 20 20 100 Disparity in stage position after aligning to target position: -0.08 -0.13 (2nd move error 0.02 0.03) Finished aligning to item in 2 rounds Trimming A by 20 20 20 85, reference by 20 20 20 85 -3.2 -1.3 unbinned pixels; 0.7 13.5 nm along two shift axes; 5.8 -12.2 nm on specimen axes Total_Shift = 6.937191 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -10.98, 6.73 unbinned pixels, unfiltered CCC is 0.0031 The average drift between shots was -0.3, 9.0 unbinned pixels, 0.16 nm/sec Measured defocus = 0.40 microns changed by -1.80 to target Tilt-induced shift is 35.08, -22.24 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was 2.2, 4.3 unbinned pixels, 0.09 nm/sec Measured defocus = -1.28 microns changed by -0.12 to target 34 frames are being saved to U:\krios\20190806\slot11_1064.tif 34 frames are being saved to U:\krios\20190806\slot11_1065.tif 34 frames are being saved to U:\krios\20190806\slot11_1066.tif Item 419: Stage: -219.95 508.61 80 Label: 417 419 Tick time from program start = 67425.863 Running script 17 at item 418 (-218.91, 506.31) Remaining time to fill dewars is 22860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 55, reference by 20 20 20 100 Disparity in stage position after aligning to target position: 0.07 -0.12 (2nd move error 0.17 0.04) Finished aligning to item in 1 rounds Trimming A by 20 20 20 50, reference by 20 20 20 55 -0.7 2.4 unbinned pixels; 9.6 1.1 nm along two shift axes; -9.1 -3.1 nm on specimen axes Total_Shift = 4.916716 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 32.32, -20.18 unbinned pixels, unfiltered CCC is 0.0028 The average drift between shots was -5.6, 11.1 unbinned pixels, 0.22 nm/sec Measured defocus = -1.18 microns changed by -0.32 to target 34 frames are being saved to U:\krios\20190806\slot11_1067.tif 34 frames are being saved to U:\krios\20190806\slot11_1068.tif 34 frames are being saved to U:\krios\20190806\slot11_1069.tif Item 420: Stage: -218.91 506.31 80 Label: 418 420 Tick time from program start = 67538.121 Running script 17 at item 419 (-217.90, 503.99) Remaining time to fill dewars is 22740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 25, reference by 20 20 20 100 Disparity in stage position after aligning to target position: 0.03 -0.11 (2nd move error 0.13 0.04) Finished aligning to item in 1 rounds Trimming A by 20 20 20 20, reference by 20 20 20 25 0.1 1.1 unbinned pixels; 4.0 1.8 nm along two shift axes; -4.3 -0.0 nm on specimen axes Total_Shift = 2.199064 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is -0.14, 0.28 unbinned pixels, unfiltered CCC is 0.0518 The average drift between shots was -7.1, 7.7 unbinned pixels, 0.19 nm/sec Measured defocus = 0.01 microns changed by -1.61 to target Tilt-induced shift is 40.27, -26.01 unbinned pixels, unfiltered CCC is 0.0339 The average drift between shots was -0.1, 5.2 unbinned pixels, 0.09 nm/sec Measured defocus = -1.48 microns changed by -0.12 to target 34 frames are being saved to U:\krios\20190806\slot11_1070.tif 34 frames are being saved to U:\krios\20190806\slot11_1071.tif 34 frames are being saved to U:\krios\20190806\slot11_1072.tif Item 421: Stage: -217.90 503.99 80 Label: 419 421 Tick time from program start = 67660.114 Running script 17 at item 420 (-214.50, 496.92) Remaining time to fill dewars is 22560 Dewars are NOT busy filling Trimming A by 20 20 20 110, reference by 20 20 20 100 Trimming A by 20 20 20 110, reference by 20 20 20 20 Trimming A by 20 20 20 110, reference by 20 20 20 105 Trimming A by 20 20 20 110, reference by 20 20 20 20 Trimming A by 20 20 20 110, reference by 23 105 20 185 Trimming A by 20 20 20 110, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.14 -0.19 Trimming A by 20 20 20 20, reference by 20 20 20 105 Disparity in stage position after aligning to target position: -0.17 -0.05 (2nd move error -0.04 0.13) Finished aligning to item in 2 rounds 0.0 31.9 unbinned pixels; 117.2 48.2 nm along two shift axes; -124.1 -6.5 nm on specimen axes Total_Shift = 63.786209 -1.0 23.5 unbinned pixels; 87.6 31.9 nm along two shift axes; -91.1 -8.7 nm on specimen axes Total_Shift = 46.997121 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 37.80, -26.12 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -5.7, 3.5 unbinned pixels, 0.12 nm/sec Measured defocus = -1.42 microns changed by -0.28 to target 34 frames are being saved to U:\krios\20190806\slot11_1073.tif 34 frames are being saved to U:\krios\20190806\slot11_1074.tif 34 frames are being saved to U:\krios\20190806\slot11_1075.tif Item 422: Stage: -214.50 496.92 80 Label: 420 422 Tick time from program start = 67790.172 Running script 17 at item 421 (-206.88, 480.77) Remaining time to fill dewars is 22380 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 20 20 105 Trimming A by 20 20 20 20, reference by 23 105 20 185 Disparity in stage position after aligning to center of map frame: -0.15 -0.18 Aligning to target with image shift equivalent to stage shift: -0.67 -0.30 Finished aligning to item in 1 rounds -20.6 127.2 unbinned pixels; 494.7 117.2 nm along two shift axes; -490.5 -107.1 nm on specimen axes Total_Shift = 257.735901 Trimming A by 20 21 20 20, reference by 20 20 20 20 10.5 66.3 unbinned pixels; 230.1 137.8 nm along two shift axes; -260.2 27.0 nm on specimen axes Total_Shift = 134.314447 Total_shift never < 120: Skiping ................................................ Running script 17 at item 422 (-209.13, 479.71) Remaining time to fill dewars is 22320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 60 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.12 -0.30 (2nd move error 0.03 -0.14) Finished aligning to item in 1 rounds Trimming A by 20 60 20 25, reference by 20 60 20 20 -18.5 -3.1 unbinned pixels; 12.8 71.2 nm along two shift axes; 15.9 -71.3 nm on specimen axes Total_Shift = 37.488042 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 4.34, -2.49 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was 2.3, 8.0 unbinned pixels, 0.15 nm/sec Measured defocus = -0.15 microns changed by -1.65 to target Tilt-induced shift is 47.59, -29.94 unbinned pixels, unfiltered CCC is -0.0021 The average drift between shots was 3.5, 4.6 unbinned pixels, 0.10 nm/sec Measured defocus = -1.74 microns changed by -0.06 to target 34 frames are being saved to U:\krios\20190806\slot11_1076.tif 34 frames are being saved to U:\krios\20190806\slot11_1077.tif 34 frames are being saved to U:\krios\20190806\slot11_1078.tif Item 424: Stage: -209.13 479.71 80 Label: 422 424 Tick time from program start = 67937.905 Running script 17 at item 423 (-210.21, 482.04) Remaining time to fill dewars is 22260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.16 -0.31 (2nd move error -0.01 -0.15) Finished aligning to item in 1 rounds -18.4 3.8 unbinned pixels; 38.0 60.4 nm along two shift axes; -11.0 -72.3 nm on specimen axes Total_Shift = 37.538005 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 42.97, -28.72 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 1.5, 5.4 unbinned pixels, 0.10 nm/sec Measured defocus = -1.60 microns changed by -0.30 to target 34 frames are being saved to U:\krios\20190806\slot11_1079.tif 34 frames are being saved to U:\krios\20190806\slot11_1080.tif 34 frames are being saved to U:\krios\20190806\slot11_1081.tif Item 425: Stage: -210.21 482.04 80 Label: 423 425 Tick time from program start = 68050.506 Running script 17 at item 424 (-211.30, 484.42) Remaining time to fill dewars is 22140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.08 -0.32 (2nd move error 0.07 -0.16) Finished aligning to item in 1 rounds -20.3 -2.0 unbinned pixels; 19.0 76.0 nm along two shift axes; 12.2 -78.4 nm on specimen axes Total_Shift = 40.708486 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 8.27, -6.32 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was 3.5, 7.1 unbinned pixels, 0.14 nm/sec Measured defocus = -0.32 microns changed by -1.68 to target Tilt-induced shift is 52.70, -33.87 unbinned pixels, unfiltered CCC is -0.0012 The average drift between shots was 3.8, 4.0 unbinned pixels, 0.10 nm/sec Measured defocus = -1.94 microns changed by -0.06 to target 34 frames are being saved to U:\krios\20190806\slot11_1082.tif 34 frames are being saved to U:\krios\20190806\slot11_1083.tif 34 frames are being saved to U:\krios\20190806\slot11_1084.tif Item 426: Stage: -211.30 484.42 80 Label: 424 426 Tick time from program start = 68173.170 Running script 17 at item 425 (-214.64, 491.16) Remaining time to fill dewars is 21960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 20 20 105 Disparity in stage position after aligning to target position: -0.05 -0.05 (2nd move error 0.09 0.11) Finished aligning to item in 1 rounds -1.1 -2.1 unbinned pixels; 6.1 7.2 nm along two shift axes; 8.3 -4.0 nm on specimen axes Total_Shift = 4.717391 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 44.95, -30.08 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was -6.3, 9.5 unbinned pixels, 0.20 nm/sec Measured defocus = -1.67 microns changed by -0.43 to target 34 frames are being saved to U:\krios\20190806\slot11_1085.tif 34 frames are being saved to U:\krios\20190806\slot11_1086.tif 34 frames are being saved to U:\krios\20190806\slot11_1087.tif Item 427: Stage: -214.64 491.16 80 Label: 425 427 Tick time from program start = 68285.865 Running script 17 at item 426 (-215.68, 493.48) Remaining time to fill dewars is 21900 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 20 20 105 Disparity in stage position after aligning to target position: -0.02 -0.06 (2nd move error 0.11 0.10) Finished aligning to item in 1 rounds -2.3 -0.3 unbinned pixels; 1.9 8.6 nm along two shift axes; 1.6 -8.8 nm on specimen axes Total_Shift = 4.567046 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 19.00, -10.91 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -11.0, 9.0 unbinned pixels, 0.25 nm/sec Measured defocus = -0.68 microns changed by -1.52 to target Tilt-induced shift is 55.37, -34.56 unbinned pixels, unfiltered CCC is -0.0011 The average drift between shots was -0.6, 4.8 unbinned pixels, 0.08 nm/sec Measured defocus = -2.02 microns changed by -0.18 to target 34 frames are being saved to U:\krios\20190806\slot11_1088.tif 34 frames are being saved to U:\krios\20190806\slot11_1089.tif 34 frames are being saved to U:\krios\20190806\slot11_1090.tif Item 428: Stage: -215.68 493.48 80 Label: 426 428 Tick time from program start = 68408.045 Running script 17 at item 427 (-216.69, 495.84) Remaining time to fill dewars is 21660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 20 20 105 Disparity in stage position after aligning to target position: -0.10 -0.13 (2nd move error 0.03 0.04) Finished aligning to item in 1 rounds -3.2 -3.3 unbinned pixels; 7.9 16.5 nm along two shift axes; 13.4 -11.8 nm on specimen axes Total_Shift = 9.182872 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 54.17, -37.15 unbinned pixels, unfiltered CCC is -0.0010 The average drift between shots was -1.2, 11.3 unbinned pixels, 0.20 nm/sec Measured defocus = -2.03 microns changed by -0.27 to target 34 frames are being saved to U:\krios\20190806\slot11_1091.tif 34 frames are being saved to U:\krios\20190806\slot11_1092.tif 34 frames are being saved to U:\krios\20190806\slot11_1093.tif Item 429: Stage: -216.69 495.84 80 Label: 427 429 Tick time from program start = 68524.157 Running script 17 at item 428 (-217.89, 492.39) Remaining time to fill dewars is 21600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 65, reference by 20 20 20 105 Disparity in stage position after aligning to target position: -0.05 -0.10 (2nd move error 0.08 0.06) Finished aligning to item in 1 rounds Trimming A by 20 20 20 60, reference by 20 20 20 65 -1.9 0.4 unbinned pixels; 4.1 6.3 nm along two shift axes; -1.3 -7.6 nm on specimen axes Total_Shift = 3.941175 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 19.40, -10.94 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -11.6, 11.8 unbinned pixels, 0.29 nm/sec Measured defocus = -0.69 microns changed by -1.71 to target Tilt-induced shift is 61.28, -39.27 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -1.2, 4.9 unbinned pixels, 0.09 nm/sec Measured defocus = -2.25 microns changed by -0.15 to target 34 frames are being saved to U:\krios\20190806\slot11_1094.tif 34 frames are being saved to U:\krios\20190806\slot11_1095.tif 34 frames are being saved to U:\krios\20190806\slot11_1096.tif Item 430: Stage: -217.89 492.39 80 Label: 428 430 Tick time from program start = 68649.675 Running script 17 at item 429 (-216.86, 490.05) Remaining time to fill dewars is 21420 Dewars are NOT busy filling Trimming A by 20 25 20 110, reference by 20 20 20 100 Trimming A by 20 25 20 110, reference by 20 20 20 20 Trimming A by 20 25 20 110, reference by 20 20 20 105 Trimming A by 20 25 20 110, reference by 20 20 20 20 Trimming A by 20 25 20 110, reference by 23 105 20 185 Trimming A by 20 25 20 110, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.09 -0.14 Trimming A by 20 20 20 40, reference by 20 20 20 105 Disparity in stage position after aligning to target position: 0.02 -0.04 (2nd move error 0.10 0.08) Finished aligning to item in 2 rounds Trimming A by 20 20 20 35, reference by 20 20 20 40 -0.0 -2.4 unbinned pixels; 8.6 3.7 nm along two shift axes; 9.2 0.3 nm on specimen axes Total_Shift = 4.7324 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 51.80, -31.93 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -4.5, 7.6 unbinned pixels, 0.16 nm/sec Measured defocus = -1.88 microns changed by -0.62 to target 34 frames are being saved to U:\krios\20190806\slot11_1097.tif 34 frames are being saved to U:\krios\20190806\slot11_1098.tif 34 frames are being saved to U:\krios\20190806\slot11_1099.tif Item 431: Stage: -216.86 490.05 80 Label: 429 431 Tick time from program start = 68771.855 Running script 17 at item 430 (-215.81, 487.75) Remaining time to fill dewars is 21360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 25, reference by 20 20 20 105 Disparity in stage position after aligning to target position: 0.12 -0.09 (2nd move error 0.20 0.03) Finished aligning to item in 1 rounds Trimming A by 20 20 20 20, reference by 20 20 20 25 1.9 1.0 unbinned pixels; 1.3 8.4 nm along two shift axes; -4.4 7.2 nm on specimen axes Total_Shift = 4.344072 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -15.88, 10.90 unbinned pixels, unfiltered CCC is -0.0019 The average drift between shots was -4.5, 5.7 unbinned pixels, 0.13 nm/sec Measured defocus = 0.59 microns changed by -1.89 to target Tilt-induced shift is 33.45, -22.05 unbinned pixels, unfiltered CCC is -0.0013 The average drift between shots was 0.5, 4.1 unbinned pixels, 0.07 nm/sec Measured defocus = -1.24 microns changed by -0.06 to target 34 frames are being saved to U:\krios\20190806\slot11_1100.tif 34 frames are being saved to U:\krios\20190806\slot11_1101.tif 34 frames are being saved to U:\krios\20190806\slot11_1102.tif Item 432: Stage: -215.81 487.75 80 Label: 430 432 Tick time from program start = 68897.545 Running script 17 at item 431 (-214.51, 485.41) Remaining time to fill dewars is 21120 Dewars are NOT busy filling Trimming A by 23 99 20 195, reference by 20 20 20 105 Trimming A by 23 99 20 195, reference by 20 20 20 20 Trimming A by 23 99 20 195, reference by 23 105 20 185 Trimming A by 23 99 20 195, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.08 -0.16 Trimming A by 20 20 20 20, reference by 23 105 20 185 Disparity in stage position after aligning to target position: -0.16 -0.02 (2nd move error -0.09 0.12) Finished aligning to item in 2 rounds 1.6 18.5 unbinned pixels; 66.0 33.6 nm along two shift axes; -72.4 2.3 nm on specimen axes Total_Shift = 37.162475 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 22.11, -12.79 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was -8.2, 2.9 unbinned pixels, 0.15 nm/sec Measured defocus = -0.79 microns changed by -0.51 to target 34 frames are being saved to U:\krios\20190806\slot11_1103.tif 34 frames are being saved to U:\krios\20190806\slot11_1104.tif 34 frames are being saved to U:\krios\20190806\slot11_1105.tif Item 433: Stage: -214.51 485.41 80 Label: 431 433 Tick time from program start = 69018.040 Running script 17 at item 432 (-213.48, 483.09) Remaining time to fill dewars is 21000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 30, reference by 23 105 20 185 Disparity in stage position after aligning to target position: -0.06 -0.07 (2nd move error 0.01 0.07) Finished aligning to item in 1 rounds Trimming A by 20 20 20 25, reference by 20 20 20 30 -0.5 -0.9 unbinned pixels; 2.6 3.2 nm along two shift axes; 3.5 -1.8 nm on specimen axes Total_Shift = 2.037709 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -7.88, 5.87 unbinned pixels, unfiltered CCC is 0.0163 The average drift between shots was -10.0, 6.2 unbinned pixels, 0.21 nm/sec Measured defocus = 0.30 microns changed by -1.70 to target Tilt-induced shift is 35.27, -22.91 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -1.3, 3.8 unbinned pixels, 0.07 nm/sec Measured defocus = -1.30 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_1106.tif 34 frames are being saved to U:\krios\20190806\slot11_1107.tif 34 frames are being saved to U:\krios\20190806\slot11_1108.tif Item 434: Stage: -213.48 483.09 80 Label: 432 434 Tick time from program start = 69139.955 Running script 17 at item 433 (-212.43, 480.98) Remaining time to fill dewars is 20880 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 20 20 105 Trimming A by 20 20 20 20, reference by 23 105 20 185 Disparity in stage position after aligning to center of map frame: -0.17 -0.16 Trimming A by 21 45 20 50, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.11 -0.35 (2nd move error 0.05 -0.21) Finished aligning to item in 2 rounds Trimming A by 21 50 30 45, reference by 21 45 20 50 -24.6 -3.3 unbinned pixels; 19.9 93.5 nm along two shift axes; 18.0 -94.9 nm on specimen axes Total_Shift = 49.598348 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is -3.87, 2.46 unbinned pixels, unfiltered CCC is -0.0031 The average drift between shots was 1.2, 8.1 unbinned pixels, 0.14 nm/sec Measured defocus = 0.14 microns changed by -1.64 to target Tilt-induced shift is 37.29, -24.51 unbinned pixels, unfiltered CCC is -0.0019 The average drift between shots was 4.3, 5.3 unbinned pixels, 0.12 nm/sec Measured defocus = -1.38 microns changed by -0.12 to target 34 frames are being saved to U:\krios\20190806\slot11_1109.tif 34 frames are being saved to U:\krios\20190806\slot11_1110.tif 34 frames are being saved to U:\krios\20190806\slot11_1111.tif Item 435: Stage: -212.43 480.98 80 Label: 433 435 Tick time from program start = 69271.620 Running script 17 at item 434 (-214.65, 479.66) Remaining time to fill dewars is 20700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 100 20 95, reference by 23 105 20 185 Disparity in stage position after aligning to target position: -0.09 -0.08 (2nd move error -0.02 0.06) Finished aligning to item in 1 rounds Trimming A by 24 100 20 95, reference by 24 100 20 95 -0.4 4.3 unbinned pixels; 16.4 5.0 nm along two shift axes; -16.7 -2.5 nm on specimen axes Total_Shift = 8.674276 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 31.77, -19.52 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -7.5, 5.1 unbinned pixels, 0.16 nm/sec Measured defocus = -1.15 microns changed by -0.45 to target 34 frames are being saved to U:\krios\20190806\slot11_1112.tif 34 frames are being saved to U:\krios\20190806\slot11_1113.tif 34 frames are being saved to U:\krios\20190806\slot11_1114.tif Item 436: Stage: -214.65 479.66 80 Label: 434 436 Tick time from program start = 69385.111 Running script 17 at item 435 (-215.68, 482.00) Remaining time to fill dewars is 20580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 60 20 90, reference by 23 105 20 185 Disparity in stage position after aligning to target position: -0.05 -0.09 (2nd move error 0.03 0.05) Finished aligning to item in 1 rounds Trimming A by 20 51 20 95, reference by 22 60 20 90 -1.0 -3.1 unbinned pixels; 10.1 8.1 nm along two shift axes; 12.2 -3.1 nm on specimen axes Total_Shift = 6.485076 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is -1.24, 2.65 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -7.3, 9.1 unbinned pixels, 0.21 nm/sec Measured defocus = 0.08 microns changed by -1.78 to target Tilt-induced shift is 43.96, -27.25 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -0.4, 3.9 unbinned pixels, 0.07 nm/sec Measured defocus = -1.60 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_1115.tif 34 frames are being saved to U:\krios\20190806\slot11_1116.tif 34 frames are being saved to U:\krios\20190806\slot11_1117.tif Item 437: Stage: -215.68 482.00 80 Label: 435 437 Tick time from program start = 69507.353 Running script 17 at item 436 (-216.69, 484.36) Remaining time to fill dewars is 20400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 30 20 90, reference by 23 105 20 185 Disparity in stage position after aligning to target position: -0.15 -0.10 (2nd move error -0.08 0.04) Finished aligning to item in 1 rounds Trimming A by 20 28 20 90, reference by 20 30 20 90 1.2 16.5 unbinned pixels; 59.2 29.2 nm along two shift axes; -64.6 1.1 nm on specimen axes Total_Shift = 33.150135 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 35.37, -22.09 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -7.1, 5.5 unbinned pixels, 0.16 nm/sec Measured defocus = -1.29 microns changed by -0.51 to target 34 frames are being saved to U:\krios\20190806\slot11_1118.tif 34 frames are being saved to U:\krios\20190806\slot11_1119.tif 34 frames are being saved to U:\krios\20190806\slot11_1120.tif Item 438: Stage: -216.69 484.36 80 Label: 436 438 Tick time from program start = 69620.906 Running script 17 at item 437 (-217.79, 486.66) Remaining time to fill dewars is 20340 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 95, reference by 23 105 20 185 Disparity in stage position after aligning to target position: -0.20 -0.11 (2nd move error -0.13 0.03) Finished aligning to item in 1 rounds Trimming A by 20 20 20 95, reference by 20 20 20 95 1.7 25.6 unbinned pixels; 91.9 44.8 nm along two shift axes; -100.0 1.3 nm on specimen axes Total_Shift = 51.344678 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 3.91, -0.80 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -8.3, 3.3 unbinned pixels, 0.16 nm/sec Measured defocus = -0.12 microns changed by -1.78 to target Tilt-induced shift is 48.16, -30.44 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -1.2, 0.7 unbinned pixels, 0.02 nm/sec Measured defocus = -1.76 microns changed by -0.14 to target 34 frames are being saved to U:\krios\20190806\slot11_1121.tif 34 frames are being saved to U:\krios\20190806\slot11_1122.tif 34 frames are being saved to U:\krios\20190806\slot11_1123.tif Item 439: Stage: -217.79 486.66 80 Label: 437 439 Tick time from program start = 69743.897 Running script 17 at item 443 (55.44, 625.05) Remaining time to fill dewars is 20100 Dewars are NOT busy filling Trimming A by 90 24 90 20, reference by 20 20 20 20 Trimming A by 90 24 90 20, reference by 75 24 70 20 Trimming A by 90 24 90 20, reference by 20 20 20 20 Trimming A by 90 24 90 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.45 -0.23 Trimming A by 115 22 85 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.41 -0.29 (2nd move error 0.03 -0.08) Finished aligning to item in 2 rounds Trimming A by 115 22 85 20, reference by 115 22 85 20 -12.2 -0.1 unbinned pixels; 15.4 44.0 nm along two shift axes; 3.1 -47.3 nm on specimen axes Total_Shift = 24.342373 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 51.61, -34.34 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -2.0, 11.0 unbinned pixels, 0.20 nm/sec Measured defocus = -1.91 microns changed by -0.09 to target 34 frames are being saved to U:\krios\20190806\slot11_1124.tif 34 frames are being saved to U:\krios\20190806\slot11_1125.tif 34 frames are being saved to U:\krios\20190806\slot11_1126.tif Item 445: Stage: 55.44 625.05 83 Label: 443 445 Tick time from program start = 69869.603 Running script 17 at item 444 (56.47, 622.66) Remaining time to fill dewars is 20040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 57 22 85 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.37 -0.28 (2nd move error 0.08 -0.07) Finished aligning to item in 1 rounds Trimming A by 75 24 70 20, reference by 57 22 85 20 -8.6 -1.1 unbinned pixels; 7.3 32.7 nm along two shift axes; 6.1 -33.3 nm on specimen axes Total_Shift = 17.383756 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 22.38, -12.78 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -0.6, 6.2 unbinned pixels, 0.11 nm/sec Measured defocus = -0.80 microns changed by -1.30 to target 34 frames are being saved to U:\krios\20190806\slot11_1127.tif 34 frames are being saved to U:\krios\20190806\slot11_1128.tif 34 frames are being saved to U:\krios\20190806\slot11_1129.tif Item 446: Stage: 56.47 622.66 83 Label: 444 446 Tick time from program start = 69982.594 Running script 17 at item 445 (57.55, 620.34) Remaining time to fill dewars is 19920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 40 20 75 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.39 -0.27 (2nd move error 0.05 -0.06) Finished aligning to item in 1 rounds Trimming A by 40 20 75 20, reference by 40 20 75 20 -7.1 -2.6 unbinned pixels; 0.2 29.3 nm along two shift axes; 11.5 -27.0 nm on specimen axes Total_Shift = 15.052308 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 59.99, -35.77 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was 1.0, 6.4 unbinned pixels, 0.12 nm/sec Measured defocus = -2.16 microns changed by -0.04 to target 34 frames are being saved to U:\krios\20190806\slot11_1130.tif 34 frames are being saved to U:\krios\20190806\slot11_1131.tif 34 frames are being saved to U:\krios\20190806\slot11_1132.tif Item 447: Stage: 57.55 620.34 83 Label: 445 447 Tick time from program start = 70095.414 Running script 17 at item 446 (58.59, 618.06) Remaining time to fill dewars is 19740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 30 20 75 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.29 -0.23 (2nd move error 0.15 -0.02) Finished aligning to item in 1 rounds Trimming A by 30 20 80 20, reference by 30 20 75 20 -1.6 -3.2 unbinned pixels; 9.7 10.7 nm along two shift axes; 12.9 -5.7 nm on specimen axes Total_Shift = 7.216279 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 27.65, -16.54 unbinned pixels, unfiltered CCC is 0.0030 The average drift between shots was -3.1, 6.1 unbinned pixels, 0.12 nm/sec Measured defocus = -1.00 microns changed by -1.30 to target 34 frames are being saved to U:\krios\20190806\slot11_1133.tif 34 frames are being saved to U:\krios\20190806\slot11_1134.tif 34 frames are being saved to U:\krios\20190806\slot11_1135.tif Item 448: Stage: 58.59 618.06 83 Label: 446 448 Tick time from program start = 70208.359 Running script 17 at item 447 (59.82, 615.58) Remaining time to fill dewars is 19560 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.34 -0.19 Trimming A by 27 20 95 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.33 -0.08 (2nd move error 0.01 0.09) Finished aligning to item in 2 rounds Trimming A by 21 20 100 20, reference by 27 20 95 20 -0.4 -0.5 unbinned pixels; 1.3 2.0 nm along two shift axes; 1.9 -1.3 nm on specimen axes Total_Shift = 1.199962 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 63.13, -40.53 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -7.7, 6.5 unbinned pixels, 0.18 nm/sec Measured defocus = -2.32 microns changed by -0.08 to target 34 frames are being saved to U:\krios\20190806\slot11_1136.tif 34 frames are being saved to U:\krios\20190806\slot11_1137.tif 34 frames are being saved to U:\krios\20190806\slot11_1138.tif Item 449: Stage: 59.82 615.58 83 Label: 447 449 Tick time from program start = 70327.591 Running script 17 at item 448 (60.76, 607.48) Remaining time to fill dewars is 19440 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 95 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.32 -0.11 (2nd move error 0.02 0.07) Finished aligning to item in 1 rounds Trimming A by 20 20 95 20, reference by 20 20 95 20 -0.8 -0.4 unbinned pixels; 0.3 3.3 nm along two shift axes; 1.6 -2.9 nm on specimen axes Total_Shift = 1.714696 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 36.47, -21.73 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -4.5, 3.5 unbinned pixels, 0.10 nm/sec Measured defocus = -1.31 microns changed by -1.19 to target 34 frames are being saved to U:\krios\20190806\slot11_1139.tif 34 frames are being saved to U:\krios\20190806\slot11_1140.tif 34 frames are being saved to U:\krios\20190806\slot11_1141.tif Item 450: Stage: 60.76 607.48 83 Label: 448 450 Tick time from program start = 70443.733 Running script 17 at item 449 (59.72, 609.79) Remaining time to fill dewars is 19380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 65 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.22 -0.14 (2nd move error 0.11 0.03) Finished aligning to item in 1 rounds Trimming A by 20 20 70 20, reference by 20 20 65 20 -0.4 1.0 unbinned pixels; 4.1 0.2 nm along two shift axes; -3.8 -1.6 nm on specimen axes Total_Shift = 2.124939 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 31.91, -21.20 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was 0.4, 4.5 unbinned pixels, 0.08 nm/sec Measured defocus = -1.18 microns changed by -0.12 to target 34 frames are being saved to U:\krios\20190806\slot11_1142.tif 34 frames are being saved to U:\krios\20190806\slot11_1143.tif 34 frames are being saved to U:\krios\20190806\slot11_1144.tif Item 451: Stage: 59.72 609.79 83 Label: 449 451 Tick time from program start = 70556.616 Running script 17 at item 450 (58.65, 612.16) Remaining time to fill dewars is 19260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 40 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.25 -0.16 (2nd move error 0.08 0.01) Finished aligning to item in 1 rounds Trimming A by 20 20 40 20, reference by 20 20 40 20 2.0 -1.6 unbinned pixels; 8.5 4.6 nm along two shift axes; 5.8 7.9 nm on specimen axes Total_Shift = 5.054629 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -0.19, 1.01 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -5.6, 4.8 unbinned pixels, 0.13 nm/sec Measured defocus = 0.02 microns changed by -1.42 to target 34 frames are being saved to U:\krios\20190806\slot11_1145.tif 34 frames are being saved to U:\krios\20190806\slot11_1146.tif 34 frames are being saved to U:\krios\20190806\slot11_1147.tif Item 452: Stage: 58.65 612.16 83 Label: 450 452 Tick time from program start = 70669.420 Running script 17 at item 451 (57.58, 614.52) Remaining time to fill dewars is 19080 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.37 -0.13 (2nd move error -0.03 0.04) Finished aligning to item in 1 rounds -1.0 32.2 unbinned pixels; 119.8 45.1 nm along two shift axes; -125.2 -10.5 nm on specimen axes Total_Shift = 64.495802 -2.5 23.9 unbinned pixels; 91.3 27.0 nm along two shift axes; -92.6 -14.8 nm on specimen axes Total_Shift = 48.129443 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 3.64, 1.21 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -6.7, -2.8 unbinned pixels, 0.13 nm/sec Measured defocus = -0.08 microns changed by -1.42 to target 34 frames are being saved to U:\krios\20190806\slot11_1148.tif 34 frames are being saved to U:\krios\20190806\slot11_1149.tif 34 frames are being saved to U:\krios\20190806\slot11_1150.tif Item 453: Stage: 57.58 614.52 83 Label: 451 453 Tick time from program start = 70788.636 Running script 17 at item 452 (56.37, 616.98) Remaining time to fill dewars is 18900 Dewars are NOT busy filling Trimming A by 90 24 90 20, reference by 20 20 20 20 Trimming A by 90 24 90 20, reference by 75 24 70 20 Trimming A by 90 24 90 20, reference by 20 20 20 20 Trimming A by 90 24 90 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.35 -0.25 Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.19 -0.26 (2nd move error 0.15 -0.03) Finished aligning to item in 2 rounds -3.0 3.0 unbinned pixels; 15.0 6.3 nm along two shift axes; -11.1 -12.4 nm on specimen axes Total_Shift = 8.530251 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 7.12, -5.49 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -3.9, 2.3 unbinned pixels, 0.08 nm/sec Measured defocus = -0.28 microns changed by -1.32 to target 34 frames are being saved to U:\krios\20190806\slot11_1151.tif 34 frames are being saved to U:\krios\20190806\slot11_1152.tif 34 frames are being saved to U:\krios\20190806\slot11_1153.tif Item 454: Stage: 56.37 616.98 83 Label: 452 454 Tick time from program start = 70908.304 Running script 17 at item 453 (55.33, 619.27) Remaining time to fill dewars is 18780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.24 -0.26 (2nd move error 0.10 -0.03) Finished aligning to item in 1 rounds -3.2 -2.5 unbinned pixels; 5.2 15.2 nm along two shift axes; 10.6 -11.7 nm on specimen axes Total_Shift = 8.108662 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 11.45, -6.39 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -1.0, 6.3 unbinned pixels, 0.11 nm/sec Measured defocus = -0.41 microns changed by -1.29 to target 34 frames are being saved to U:\krios\20190806\slot11_1154.tif 34 frames are being saved to U:\krios\20190806\slot11_1155.tif 34 frames are being saved to U:\krios\20190806\slot11_1156.tif Item 455: Stage: 55.33 619.27 83 Label: 453 455 Tick time from program start = 71021.608 Running script 17 at item 454 (54.27, 621.58) Remaining time to fill dewars is 18660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.31 -0.29 (2nd move error 0.03 -0.06) Finished aligning to item in 1 rounds -8.5 -1.8 unbinned pixels; 4.5 33.1 nm along two shift axes; 8.7 -32.5 nm on specimen axes Total_Shift = 17.275951 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 13.39, -8.36 unbinned pixels, unfiltered CCC is 0.0014 The average drift between shots was 0.0, 6.9 unbinned pixels, 0.12 nm/sec Measured defocus = -0.49 microns changed by -1.31 to target 34 frames are being saved to U:\krios\20190806\slot11_1157.tif 34 frames are being saved to U:\krios\20190806\slot11_1158.tif 34 frames are being saved to U:\krios\20190806\slot11_1159.tif Item 456: Stage: 54.27 621.58 83 Label: 454 456 Tick time from program start = 71134.553 Running script 17 at item 455 (53.19, 623.97) Remaining time to fill dewars is 18540 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 60 20 40 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.35 -0.29 (2nd move error -0.01 -0.06) Finished aligning to item in 1 rounds Trimming A by 60 20 40 20, reference by 60 20 40 20 -7.6 3.0 unbinned pixels; 20.9 23.1 nm along two shift axes; -9.9 -30.4 nm on specimen axes Total_Shift = 16.401523 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 14.20, -8.68 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -1.1, 1.8 unbinned pixels, 0.04 nm/sec Measured defocus = -0.51 microns changed by -1.39 to target 34 frames are being saved to U:\krios\20190806\slot11_1160.tif 34 frames are being saved to U:\krios\20190806\slot11_1161.tif 34 frames are being saved to U:\krios\20190806\slot11_1162.tif Item 457: Stage: 53.19 623.97 83 Label: 455 457 Tick time from program start = 71247.794 Running script 17 at item 456 (49.89, 624.99) Remaining time to fill dewars is 18480 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.43 -0.15 Trimming A by 70 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.36 -0.05 (2nd move error 0.06 0.08) Finished aligning to item in 2 rounds Trimming A by 70 20 30 20, reference by 70 20 20 20 0.7 -0.5 unbinned pixels; 2.8 1.8 nm along two shift axes; 1.9 2.9 nm on specimen axes Total_Shift = 1.769759 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 48.17, -31.45 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -3.4, 10.3 unbinned pixels, 0.19 nm/sec Measured defocus = -1.78 microns changed by -0.22 to target 34 frames are being saved to U:\krios\20190806\slot11_1163.tif 34 frames are being saved to U:\krios\20190806\slot11_1164.tif 34 frames are being saved to U:\krios\20190806\slot11_1165.tif Item 458: Stage: 49.89 624.99 83 Label: 456 458 Tick time from program start = 71371.424 Running script 17 at item 457 (50.95, 622.83) Remaining time to fill dewars is 18180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.29 -0.34 (2nd move error 0.05 -0.11) Finished aligning to item in 1 rounds -13.5 -2.4 unbinned pixels; 8.8 52.1 nm along two shift axes; 12.1 -51.9 nm on specimen axes Total_Shift = 27.346017 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 17.76, -11.63 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was 2.8, 9.6 unbinned pixels, 0.18 nm/sec Measured defocus = -0.66 microns changed by -1.44 to target 34 frames are being saved to U:\krios\20190806\slot11_1166.tif 34 frames are being saved to U:\krios\20190806\slot11_1167.tif 34 frames are being saved to U:\krios\20190806\slot11_1168.tif Item 459: Stage: 50.95 622.83 83 Label: 457 459 Tick time from program start = 71487.520 Running script 17 at item 458 (52.01, 620.55) Remaining time to fill dewars is 18120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.28 -0.35 (2nd move error 0.06 -0.12) Finished aligning to item in 1 rounds -14.0 -0.3 unbinned pixels; 17.3 51.0 nm along two shift axes; 4.1 -54.6 nm on specimen axes Total_Shift = 28.08865 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 55.64, -39.05 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was 3.7, 10.9 unbinned pixels, 0.21 nm/sec Measured defocus = -2.10 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_1169.tif 34 frames are being saved to U:\krios\20190806\slot11_1170.tif 34 frames are being saved to U:\krios\20190806\slot11_1171.tif Item 460: Stage: 52.01 620.55 83 Label: 458 460 Tick time from program start = 71600.075 Running script 17 at item 459 (54.29, 615.81) Remaining time to fill dewars is 18000 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.14 Disparity in stage position after aligning to target position: -0.40 -0.18 (2nd move error -0.11 -0.06) Finished aligning to item in 2 rounds -7.8 22.8 unbinned pixels; 94.1 6.4 nm along two shift axes; -87.1 -35.0 nm on specimen axes Total_Shift = 48.215792 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 29.66, -17.87 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -4.4, 1.9 unbinned pixels, 0.08 nm/sec Measured defocus = -1.07 microns changed by -1.23 to target 34 frames are being saved to U:\krios\20190806\slot11_1172.tif 34 frames are being saved to U:\krios\20190806\slot11_1173.tif 34 frames are being saved to U:\krios\20190806\slot11_1174.tif Item 461: Stage: 54.29 615.81 83 Label: 459 461 Tick time from program start = 71722.770 Running script 17 at item 460 (55.31, 613.43) Remaining time to fill dewars is 17820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.29 -0.18 (2nd move error -0.00 -0.06) Finished aligning to item in 1 rounds -8.6 1.3 unbinned pixels; 15.9 29.2 nm along two shift axes; -3.1 -33.9 nm on specimen axes Total_Shift = 17.453577 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 64.86, -39.98 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -1.4, 5.9 unbinned pixels, 0.11 nm/sec Measured defocus = -2.36 microns changed by -0.04 to target 34 frames are being saved to U:\krios\20190806\slot11_1175.tif 34 frames are being saved to U:\krios\20190806\slot11_1176.tif 34 frames are being saved to U:\krios\20190806\slot11_1177.tif Item 462: Stage: 55.31 613.43 83 Label: 460 462 Tick time from program start = 71835.153 Running script 17 at item 461 (56.37, 611.07) Remaining time to fill dewars is 17700 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.23 -0.16 (2nd move error 0.05 -0.04) Finished aligning to item in 1 rounds -5.4 0.2 unbinned pixels; 7.6 19.1 nm along two shift axes; 0.5 -20.9 nm on specimen axes Total_Shift = 10.718581 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 33.49, -20.65 unbinned pixels, unfiltered CCC is 0.0013 The average drift between shots was -1.5, 5.2 unbinned pixels, 0.10 nm/sec Measured defocus = -1.22 microns changed by -1.28 to target 34 frames are being saved to U:\krios\20190806\slot11_1178.tif 34 frames are being saved to U:\krios\20190806\slot11_1179.tif 34 frames are being saved to U:\krios\20190806\slot11_1180.tif Item 463: Stage: 56.37 611.07 83 Label: 461 463 Tick time from program start = 71947.521 Running script 17 at item 462 (58.55, 606.41) Remaining time to fill dewars is 17580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.18 -0.12 (2nd move error 0.10 -0.00) Finished aligning to item in 1 rounds -1.2 -1.8 unbinned pixels; 4.8 7.1 nm along two shift axes; 7.1 -4.5 nm on specimen axes Total_Shift = 4.301422 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 40.08, -25.22 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -3.1, 6.5 unbinned pixels, 0.13 nm/sec Measured defocus = -1.46 microns changed by 0.16 to target 34 frames are being saved to U:\krios\20190806\slot11_1181.tif 34 frames are being saved to U:\krios\20190806\slot11_1182.tif 34 frames are being saved to U:\krios\20190806\slot11_1183.tif Item 464: Stage: 58.55 606.41 83 Label: 462 464 Tick time from program start = 72063.461 Running script 17 at item 463 (59.58, 604.12) Remaining time to fill dewars is 17460 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Skipping first round alignment to center of map frame Trimming A by 20 20 45 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.12 -0.09 (2nd move error 0.17 0.03) Finished aligning to item in 1 rounds Trimming A by 20 20 50 20, reference by 20 20 45 20 -0.1 1.8 unbinned pixels; 6.7 2.2 nm along two shift axes; -6.9 -0.8 nm on specimen axes Total_Shift = 3.55999 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 5.70, -0.74 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -6.9, 3.6 unbinned pixels, 0.14 nm/sec Measured defocus = -0.16 microns changed by -1.14 to target 34 frames are being saved to U:\krios\20190806\slot11_1184.tif 34 frames are being saved to U:\krios\20190806\slot11_1185.tif 34 frames are being saved to U:\krios\20190806\slot11_1186.tif Item 465: Stage: 59.58 604.12 83 Label: 463 465 Tick time from program start = 72193.534 Running script 17 at item 464 (60.91, 601.73) Remaining time to fill dewars is 17280 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 158 20 20 Trimming A by 20 20 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.19 Trimming A by 20 20 85 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.41 -0.06 (2nd move error -0.13 0.10) Finished aligning to item in 2 rounds Trimming A by 20 20 85 20, reference by 20 20 85 20 2.3 26.2 unbinned pixels; 93.4 47.6 nm along two shift axes; -102.5 3.3 nm on specimen axes Total_Shift = 52.633338 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -285.01, -4.27 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was 286.4, 6.0 unbinned pixels, 5.06 nm/sec Measured defocus = 7.51 microns changed by -8.91 to target Tilt-induced shift is 250.15, -154.44 unbinned pixels, unfiltered CCC is 0.0008 The average drift between shots was -4.0, 0.7 unbinned pixels, 0.07 nm/sec Measured defocus = -9.12 microns changed by 7.72 to target Tilt-induced shift is 147.80, -91.75 unbinned pixels, unfiltered CCC is -0.0010 The average drift between shots was -2.3, 0.6 unbinned pixels, 0.04 nm/sec Measured defocus = -5.39 microns changed by 3.99 to target Tilt-induced shift is 47.40, -29.73 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was -1.9, 0.3 unbinned pixels, 0.03 nm/sec Measured defocus = -1.73 microns changed by 0.33 to target 34 frames are being saved to U:\krios\20190806\slot11_1187.tif 34 frames are being saved to U:\krios\20190806\slot11_1188.tif 34 frames are being saved to U:\krios\20190806\slot11_1189.tif Item 466: Stage: 60.91 601.73 83 Label: 464 466 Tick time from program start = 72342.624 Running script 17 at item 465 (61.97, 599.40) Remaining time to fill dewars is 17100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 115 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.32 -0.10 (2nd move error -0.03 0.06) Finished aligning to item in 1 rounds Trimming A by 20 20 120 20, reference by 20 20 115 20 -0.3 38.8 unbinned pixels; 142.9 57.4 nm along two shift axes; -150.8 -9.2 nm on specimen axes Total_Shift = 77.55883 Trimming A by 20 20 120 20, reference by 20 20 115 20 -1.9 29.1 unbinned pixels; 109.6 37.0 nm along two shift axes; -112.9 -13.5 nm on specimen axes Total_Shift = 58.362428 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 30.65, -47.18 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -30.9, 46.9 unbinned pixels, 1.00 nm/sec Measured defocus = -1.57 microns changed by 0.07 to target 34 frames are being saved to U:\krios\20190806\slot11_1190.tif 34 frames are being saved to U:\krios\20190806\slot11_1191.tif 34 frames are being saved to U:\krios\20190806\slot11_1192.tif Item 467: Stage: 61.97 599.40 83 Label: 465 467 Tick time from program start = 72462.012 Running script 17 at item 466 (61.79, 593.62) Remaining time to fill dewars is 16980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 23 23 130 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.23 -0.09 (2nd move error 0.05 0.07) Finished aligning to item in 1 rounds Trimming A by 22 22 130 20, reference by 23 23 130 20 -0.6 -1.0 unbinned pixels; 2.8 3.8 nm along two shift axes; 4.0 -2.2 nm on specimen axes Total_Shift = 2.345526 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 13.38, -5.72 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -7.3, 4.5 unbinned pixels, 0.15 nm/sec Measured defocus = -0.45 microns changed by -1.15 to target 34 frames are being saved to U:\krios\20190806\slot11_1193.tif 34 frames are being saved to U:\krios\20190806\slot11_1194.tif 34 frames are being saved to U:\krios\20190806\slot11_1195.tif Item 468: Stage: 61.79 593.62 83 Label: 466 468 Tick time from program start = 72574.457 Running script 17 at item 467 (60.78, 595.96) Remaining time to fill dewars is 16920 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 85 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.30 -0.11 (2nd move error -0.02 0.05) Finished aligning to item in 1 rounds Trimming A by 20 20 85 20, reference by 20 20 85 20 -0.2 4.0 unbinned pixels; 15.0 5.4 nm along two shift axes; -15.6 -1.6 nm on specimen axes Total_Shift = 8.025828 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 14.39, -8.12 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -7.4, -0.9 unbinned pixels, 0.13 nm/sec Measured defocus = -0.51 microns changed by -1.19 to target 34 frames are being saved to U:\krios\20190806\slot11_1196.tif 34 frames are being saved to U:\krios\20190806\slot11_1197.tif 34 frames are being saved to U:\krios\20190806\slot11_1198.tif Item 469: Stage: 60.78 595.96 83 Label: 467 469 Tick time from program start = 72687.761 Running script 17 at item 468 (59.74, 598.27) Remaining time to fill dewars is 16680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 45 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.24 -0.13 (2nd move error 0.05 0.04) Finished aligning to item in 1 rounds Trimming A by 20 20 45 20, reference by 20 20 45 20 -0.4 -0.4 unbinned pixels; 1.1 2.1 nm along two shift axes; 1.8 -1.4 nm on specimen axes Total_Shift = 1.180299 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 16.27, -8.34 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was -6.5, 3.9 unbinned pixels, 0.13 nm/sec Measured defocus = -0.56 microns changed by -1.24 to target 34 frames are being saved to U:\krios\20190806\slot11_1199.tif 34 frames are being saved to U:\krios\20190806\slot11_1200.tif 34 frames are being saved to U:\krios\20190806\slot11_1201.tif Item 470: Stage: 59.74 598.27 83 Label: 468 470 Tick time from program start = 72800.518 Running script 17 at item 469 (58.65, 600.66) Remaining time to fill dewars is 16620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.30 -0.13 (2nd move error -0.01 0.03) Finished aligning to item in 1 rounds 0.7 2.6 unbinned pixels; 8.7 6.6 nm along two shift axes; -10.4 2.3 nm on specimen axes Total_Shift = 5.456785 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 21.85, -12.88 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -8.1, 0.9 unbinned pixels, 0.14 nm/sec Measured defocus = -0.78 microns changed by -1.12 to target 34 frames are being saved to U:\krios\20190806\slot11_1202.tif 34 frames are being saved to U:\krios\20190806\slot11_1203.tif 34 frames are being saved to U:\krios\20190806\slot11_1204.tif Item 471: Stage: 58.65 600.66 83 Label: 469 471 Tick time from program start = 72913.198 Running script 17 at item 470 (57.34, 603.06) Remaining time to fill dewars is 16500 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.23 -0.19 Disparity in stage position after aligning to target position: -0.13 -0.16 (2nd move error 0.09 0.01) Finished aligning to item in 2 rounds 1.2 -4.4 unbinned pixels; 17.6 2.3 nm along two shift axes; 16.7 5.5 nm on specimen axes Total_Shift = 9.038045 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 28.94, -15.47 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -6.3, 6.1 unbinned pixels, 0.16 nm/sec Measured defocus = -1.01 microns changed by -0.99 to target 34 frames are being saved to U:\krios\20190806\slot11_1205.tif 34 frames are being saved to U:\krios\20190806\slot11_1206.tif 34 frames are being saved to U:\krios\20190806\slot11_1207.tif Item 472: Stage: 57.34 603.06 83 Label: 470 472 Tick time from program start = 73035.128 Running script 17 at item 471 (56.31, 605.33) Remaining time to fill dewars is 16320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.10 -0.18 (2nd move error 0.12 -0.01) Finished aligning to item in 1 rounds -2.1 -0.2 unbinned pixels; 2.1 7.7 nm along two shift axes; 1.0 -8.0 nm on specimen axes Total_Shift = 4.15299 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 32.72, -18.47 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -2.3, 4.6 unbinned pixels, 0.09 nm/sec Measured defocus = -1.16 microns changed by -0.94 to target 34 frames are being saved to U:\krios\20190806\slot11_1208.tif 34 frames are being saved to U:\krios\20190806\slot11_1209.tif 34 frames are being saved to U:\krios\20190806\slot11_1210.tif Item 473: Stage: 56.31 605.33 83 Label: 471 473 Tick time from program start = 73147.839 Running script 17 at item 472 (55.20, 607.66) Remaining time to fill dewars is 16140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.14 -0.21 (2nd move error 0.08 -0.04) Finished aligning to item in 1 rounds -5.3 -2.0 unbinned pixels; 0.5 22.0 nm along two shift axes; 8.9 -20.1 nm on specimen axes Total_Shift = 11.299315 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 31.09, -17.60 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was 1.3, 4.8 unbinned pixels, 0.09 nm/sec Measured defocus = -1.10 microns changed by -1.10 to target 34 frames are being saved to U:\krios\20190806\slot11_1211.tif 34 frames are being saved to U:\krios\20190806\slot11_1212.tif 34 frames are being saved to U:\krios\20190806\slot11_1213.tif Item 474: Stage: 55.20 607.66 83 Label: 472 474 Tick time from program start = 73264.403 Running script 17 at item 473 (43.15, 605.32) Remaining time to fill dewars is 16020 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.34 -0.14 Disparity in stage position after aligning to target position: -0.32 -0.21 (2nd move error 0.02 -0.10) Finished aligning to item in 2 rounds -12.4 -1.8 unbinned pixels; 9.7 47.5 nm along two shift axes; 9.6 -48.0 nm on specimen axes Total_Shift = 25.140832 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 79.48, -51.42 unbinned pixels, unfiltered CCC is 0.0071 The average drift between shots was 3.5, 7.4 unbinned pixels, 0.15 nm/sec Measured defocus = -2.93 microns changed by 0.63 to target 34 frames are being saved to U:\krios\20190806\slot11_1214.tif 34 frames are being saved to U:\krios\20190806\slot11_1215.tif 34 frames are being saved to U:\krios\20190806\slot11_1216.tif Item 475: Stage: 43.15 605.32 83 Label: 473 475 Tick time from program start = 73384.961 Running script 17 at item 474 (53.07, 612.35) Remaining time to fill dewars is 15960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.30 -0.22 (2nd move error -0.07 -0.05) Finished aligning to item in 1 rounds -7.4 15.1 unbinned pixels; 65.4 3.9 nm along two shift axes; -57.3 -32.0 nm on specimen axes Total_Shift = 33.718571 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 30.48, -17.47 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -4.7, 2.0 unbinned pixels, 0.09 nm/sec Measured defocus = -1.09 microns changed by -1.31 to target 34 frames are being saved to U:\krios\20190806\slot11_1217.tif 34 frames are being saved to U:\krios\20190806\slot11_1218.tif 34 frames are being saved to U:\krios\20190806\slot11_1219.tif Item 476: Stage: 53.07 612.35 83 Label: 474 476 Tick time from program start = 73496.579 Running script 17 at item 475 (51.99, 614.72) Remaining time to fill dewars is 15780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.31 -0.21 (2nd move error -0.08 -0.04) Finished aligning to item in 1 rounds -5.2 16.5 unbinned pixels; 67.3 6.1 nm along two shift axes; -62.9 -23.6 nm on specimen axes Total_Shift = 34.513673 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 70.59, -44.74 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -0.9, 4.0 unbinned pixels, 0.07 nm/sec Measured defocus = -2.58 microns changed by 0.08 to target 34 frames are being saved to U:\krios\20190806\slot11_1220.tif 34 frames are being saved to U:\krios\20190806\slot11_1221.tif 34 frames are being saved to U:\krios\20190806\slot11_1222.tif Item 477: Stage: 51.99 614.72 83 Label: 475 477 Tick time from program start = 73609.727 Running script 17 at item 476 (50.90, 617.22) Remaining time to fill dewars is 15600 Dewars are NOT busy filling Trimming A by 85 20 90 20, reference by 20 20 20 20 Trimming A by 85 20 90 20, reference by 75 24 70 20 Trimming A by 85 20 90 20, reference by 20 20 20 20 Trimming A by 85 20 90 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.20 Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to target position: -0.20 -0.35 (2nd move error 0.11 -0.17) Finished aligning to item in 2 rounds -21.0 -3.8 unbinned pixels; 13.3 81.2 nm along two shift axes; 19.3 -80.8 nm on specimen axes Total_Shift = 42.628314 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -1.06, 0.43 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was 2.7, 6.7 unbinned pixels, 0.13 nm/sec Measured defocus = 0.04 microns changed by -1.34 to target 34 frames are being saved to U:\krios\20190806\slot11_1223.tif 34 frames are being saved to U:\krios\20190806\slot11_1224.tif 34 frames are being saved to U:\krios\20190806\slot11_1225.tif Item 478: Stage: 50.90 617.22 83 Label: 476 478 Tick time from program start = 73731.033 Running script 17 at item 477 (49.79, 619.22) Remaining time to fill dewars is 15480 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.41 -0.17 Disparity in stage position after aligning to target position: -0.20 -0.09 (2nd move error 0.20 0.06) Finished aligning to item in 2 rounds -1.4 -2.2 unbinned pixels; 6.3 8.5 nm along two shift axes; 8.9 -5.2 nm on specimen axes Total_Shift = 5.291748 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is -0.25, 0.42 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -4.3, 8.1 unbinned pixels, 0.16 nm/sec Measured defocus = 0.01 microns changed by -1.41 to target 34 frames are being saved to U:\krios\20190806\slot11_1226.tif 34 frames are being saved to U:\krios\20190806\slot11_1227.tif 34 frames are being saved to U:\krios\20190806\slot11_1228.tif Item 479: Stage: 49.79 619.22 83 Label: 477 479 Tick time from program start = 73854.040 Running script 17 at item 478 (48.73, 621.52) Remaining time to fill dewars is 15360 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.23 -0.11 (2nd move error 0.17 0.03) Finished aligning to item in 1 rounds -0.1 5.2 unbinned pixels; 19.2 7.5 nm along two shift axes; -20.1 -1.4 nm on specimen axes Total_Shift = 10.36655 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 2.38, 1.62 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was -6.4, -0.9 unbinned pixels, 0.11 nm/sec Measured defocus = -0.04 microns changed by -1.46 to target 34 frames are being saved to U:\krios\20190806\slot11_1229.tif 34 frames are being saved to U:\krios\20190806\slot11_1230.tif 34 frames are being saved to U:\krios\20190806\slot11_1231.tif Item 480: Stage: 48.73 621.52 83 Label: 478 480 Tick time from program start = 73967.188 Running script 17 at item 479 (47.71, 623.87) Remaining time to fill dewars is 15240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 35 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.27 -0.10 (2nd move error 0.13 0.05) Finished aligning to item in 1 rounds Trimming A by 35 20 20 20, reference by 35 20 20 20 -0.8 -2.9 unbinned pixels; 9.7 7.4 nm along two shift axes; 11.6 -2.6 nm on specimen axes Total_Shift = 6.08597 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 4.10, 0.30 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was -8.5, 5.5 unbinned pixels, 0.18 nm/sec Measured defocus = -0.10 microns changed by -1.50 to target 34 frames are being saved to U:\krios\20190806\slot11_1232.tif 34 frames are being saved to U:\krios\20190806\slot11_1233.tif 34 frames are being saved to U:\krios\20190806\slot11_1234.tif Item 481: Stage: 47.71 623.87 83 Label: 479 481 Tick time from program start = 74080.600 Running script 17 at item 480 (44.37, 625.11) Remaining time to fill dewars is 15120 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 80 20 20 25, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.37 -0.15 (2nd move error 0.03 -0.01) Finished aligning to item in 1 rounds Trimming A by 80 20 20 25, reference by 80 20 20 25 -2.6 -2.3 unbinned pixels; 4.9 12.9 nm along two shift axes; 9.4 -9.8 nm on specimen axes Total_Shift = 6.953155 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 6.24, -1.10 unbinned pixels, unfiltered CCC is 0.0027 The average drift between shots was -0.8, 0.7 unbinned pixels, 0.02 nm/sec Measured defocus = -0.18 microns changed by -1.52 to target Tilt-induced shift is 43.89, -27.74 unbinned pixels, unfiltered CCC is 0.0026 The average drift between shots was 1.1, 2.9 unbinned pixels, 0.06 nm/sec Measured defocus = -1.60 microns changed by -0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_1235.tif 34 frames are being saved to U:\krios\20190806\slot11_1236.tif 34 frames are being saved to U:\krios\20190806\slot11_1237.tif Item 482: Stage: 44.37 625.11 83 Label: 480 482 Tick time from program start = 74203.576 Running script 17 at item 481 (45.46, 622.78) Remaining time to fill dewars is 14940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.25 -0.13 (2nd move error 0.15 0.01) Finished aligning to item in 1 rounds 1.4 -3.0 unbinned pixels; 12.7 0.4 nm along two shift axes; 11.3 5.9 nm on specimen axes Total_Shift = 6.531664 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 43.67, -27.44 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -3.3, 6.4 unbinned pixels, 0.13 nm/sec Measured defocus = -1.59 microns changed by -0.21 to target 34 frames are being saved to U:\krios\20190806\slot11_1238.tif 34 frames are being saved to U:\krios\20190806\slot11_1239.tif 34 frames are being saved to U:\krios\20190806\slot11_1240.tif Item 483: Stage: 45.46 622.78 83 Label: 481 483 Tick time from program start = 74316.068 Running script 17 at item 482 (46.48, 620.43) Remaining time to fill dewars is 14820 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.22 -0.17 (2nd move error 0.18 -0.02) Finished aligning to item in 1 rounds -2.1 -2.9 unbinned pixels; 8.1 11.8 nm along two shift axes; 11.8 -7.4 nm on specimen axes Total_Shift = 7.170289 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 11.43, -5.49 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -1.1, 6.2 unbinned pixels, 0.11 nm/sec Measured defocus = -0.39 microns changed by -1.51 to target Tilt-induced shift is 51.44, -32.29 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was 1.9, 4.0 unbinned pixels, 0.08 nm/sec Measured defocus = -1.88 microns changed by -0.02 to target 34 frames are being saved to U:\krios\20190806\slot11_1241.tif 34 frames are being saved to U:\krios\20190806\slot11_1242.tif 34 frames are being saved to U:\krios\20190806\slot11_1243.tif Item 484: Stage: 46.48 620.43 83 Label: 482 484 Tick time from program start = 74438.186 Running script 17 at item 483 (47.53, 618.15) Remaining time to fill dewars is 14760 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.14 -0.17 (2nd move error 0.26 -0.02) Finished aligning to item in 1 rounds -2.1 -7.3 unbinned pixels; 24.2 18.7 nm along two shift axes; 29.0 -6.8 nm on specimen axes Total_Shift = 15.301013 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 53.49, -35.95 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was 0.4, 10.6 unbinned pixels, 0.19 nm/sec Measured defocus = -1.99 microns changed by -0.01 to target 34 frames are being saved to U:\krios\20190806\slot11_1244.tif 34 frames are being saved to U:\krios\20190806\slot11_1245.tif 34 frames are being saved to U:\krios\20190806\slot11_1246.tif Item 485: Stage: 47.53 618.15 83 Label: 483 485 Tick time from program start = 74551.583 Running script 17 at item 484 (49.84, 613.45) Remaining time to fill dewars is 14580 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.26 -0.18 Disparity in stage position after aligning to target position: -0.28 -0.06 (2nd move error -0.03 0.10) Finished aligning to item in 2 rounds 0.7 7.3 unbinned pixels; 26.0 13.6 nm along two shift axes; -28.6 1.3 nm on specimen axes Total_Shift = 14.698933 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 22.62, -12.98 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -10.4, 3.3 unbinned pixels, 0.19 nm/sec Measured defocus = -0.81 microns changed by -1.29 to target 34 frames are being saved to U:\krios\20190806\slot11_1247.tif 34 frames are being saved to U:\krios\20190806\slot11_1248.tif 34 frames are being saved to U:\krios\20190806\slot11_1249.tif Item 486: Stage: 49.84 613.45 83 Label: 484 486 Tick time from program start = 74671.548 Running script 17 at item 485 (50.82, 611.15) Remaining time to fill dewars is 14400 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.18 -0.06 (2nd move error 0.07 0.11) Finished aligning to item in 1 rounds 0.3 -1.8 unbinned pixels; 7.1 1.8 nm along two shift axes; 7.0 1.4 nm on specimen axes Total_Shift = 3.683252 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 63.16, -39.54 unbinned pixels, unfiltered CCC is -0.0006 The average drift between shots was -6.0, 7.6 unbinned pixels, 0.17 nm/sec Measured defocus = -2.30 microns changed by 0.10 to target 34 frames are being saved to U:\krios\20190806\slot11_1250.tif 34 frames are being saved to U:\krios\20190806\slot11_1251.tif 34 frames are being saved to U:\krios\20190806\slot11_1252.tif Item 487: Stage: 50.82 611.15 83 Label: 485 487 Tick time from program start = 74783.978 Running script 17 at item 486 (51.90, 608.92) Remaining time to fill dewars is 14280 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.10 Disparity in stage position after aligning to target position: -0.23 -0.22 (2nd move error 0.02 -0.15) Finished aligning to item in 2 rounds -21.5 -1.3 unbinned pixels; 23.1 79.3 nm along two shift axes; 9.8 -83.2 nm on specimen axes Total_Shift = 43.0068 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 31.88, -20.57 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was 5.9, 4.0 unbinned pixels, 0.13 nm/sec Measured defocus = -1.17 microns changed by -1.13 to target 34 frames are being saved to U:\krios\20190806\slot11_1253.tif 34 frames are being saved to U:\krios\20190806\slot11_1254.tif 34 frames are being saved to U:\krios\20190806\slot11_1255.tif Item 488: Stage: 51.90 608.92 83 Label: 486 488 Tick time from program start = 74904.457 Running script 17 at item 487 (52.99, 606.58) Remaining time to fill dewars is 14160 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.17 -0.21 (2nd move error 0.08 -0.13) Finished aligning to item in 1 rounds -16.1 -2.3 unbinned pixels; 12.8 61.4 nm along two shift axes; 12.2 -62.2 nm on specimen axes Total_Shift = 32.555703 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 76.76, -50.62 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was 5.3, 7.5 unbinned pixels, 0.16 nm/sec Measured defocus = -2.84 microns changed by 0.44 to target 34 frames are being saved to U:\krios\20190806\slot11_1256.tif 34 frames are being saved to U:\krios\20190806\slot11_1257.tif 34 frames are being saved to U:\krios\20190806\slot11_1258.tif Item 489: Stage: 52.99 606.58 83 Label: 487 489 Tick time from program start = 75017.417 Running script 17 at item 488 (54.06, 604.31) Remaining time to fill dewars is 14040 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.12 -0.20 (2nd move error 0.14 -0.12) Finished aligning to item in 1 rounds -14.6 -2.4 unbinned pixels; 10.0 56.2 nm along two shift axes; 12.6 -56.2 nm on specimen axes Total_Shift = 29.56228 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 44.92, -28.72 unbinned pixels, unfiltered CCC is -0.0002 The average drift between shots was 1.0, 7.1 unbinned pixels, 0.13 nm/sec Measured defocus = -1.65 microns changed by -0.85 to target 34 frames are being saved to U:\krios\20190806\slot11_1259.tif 34 frames are being saved to U:\krios\20190806\slot11_1260.tif 34 frames are being saved to U:\krios\20190806\slot11_1261.tif Item 490: Stage: 54.06 604.31 83 Label: 488 490 Tick time from program start = 75130.893 Running script 17 at item 489 (55.38, 601.98) Remaining time to fill dewars is 13920 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 158 20 20 Trimming A by 20 20 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.13 Disparity in stage position after aligning to target position: -0.33 -0.16 (2nd move error -0.09 -0.05) Finished aligning to item in 2 rounds -11.0 23.3 unbinned pixels; 100.1 4.4 nm along two shift axes; -88.5 -47.6 nm on specimen axes Total_Shift = 51.568774 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 46.96, -30.26 unbinned pixels, unfiltered CCC is 0.0025 The average drift between shots was -2.4, 1.2 unbinned pixels, 0.05 nm/sec Measured defocus = -1.73 microns changed by 0.43 to target 34 frames are being saved to U:\krios\20190806\slot11_1262.tif 34 frames are being saved to U:\krios\20190806\slot11_1263.tif 34 frames are being saved to U:\krios\20190806\slot11_1264.tif Item 491: Stage: 55.38 601.98 83 Label: 489 491 Tick time from program start = 75254.040 Running script 17 at item 490 (56.43, 599.58) Remaining time to fill dewars is 13740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.29 -0.19 (2nd move error -0.05 -0.08) Finished aligning to item in 1 rounds -10.8 9.6 unbinned pixels; 49.6 24.5 nm along two shift axes; -35.2 -44.2 nm on specimen axes Total_Shift = 29.037167 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 6.40, -1.16 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -4.9, -1.2 unbinned pixels, 0.09 nm/sec Measured defocus = -0.19 microns changed by -1.11 to target 34 frames are being saved to U:\krios\20190806\slot11_1265.tif 34 frames are being saved to U:\krios\20190806\slot11_1266.tif 34 frames are being saved to U:\krios\20190806\slot11_1267.tif Item 492: Stage: 56.43 599.58 83 Label: 490 492 Tick time from program start = 75367.078 Running script 17 at item 491 (57.48, 597.19) Remaining time to fill dewars is 13680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.27 -0.16 (2nd move error -0.03 -0.05) Finished aligning to item in 1 rounds -10.0 7.9 unbinned pixels; 42.2 24.1 nm along two shift axes; -28.7 -40.6 nm on specimen axes Total_Shift = 25.534901 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 7.81, -2.05 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -4.6, 0.4 unbinned pixels, 0.08 nm/sec Measured defocus = -0.24 microns changed by -1.16 to target 34 frames are being saved to U:\krios\20190806\slot11_1268.tif 34 frames are being saved to U:\krios\20190806\slot11_1269.tif 34 frames are being saved to U:\krios\20190806\slot11_1270.tif Item 493: Stage: 57.48 597.19 83 Label: 491 493 Tick time from program start = 75479.617 Running script 17 at item 492 (58.53, 594.87) Remaining time to fill dewars is 13500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.19 -0.17 (2nd move error 0.06 -0.06) Finished aligning to item in 1 rounds -8.7 -2.2 unbinned pixels; 3.1 34.7 nm along two shift axes; 10.5 -33.4 nm on specimen axes Total_Shift = 17.979685 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 11.52, -5.24 unbinned pixels, unfiltered CCC is -0.0007 The average drift between shots was -0.6, 4.9 unbinned pixels, 0.09 nm/sec Measured defocus = -0.39 microns changed by -1.11 to target 34 frames are being saved to U:\krios\20190806\slot11_1271.tif 34 frames are being saved to U:\krios\20190806\slot11_1272.tif 34 frames are being saved to U:\krios\20190806\slot11_1273.tif Item 494: Stage: 58.53 594.87 83 Label: 492 494 Tick time from program start = 75592.874 Running script 17 at item 493 (59.60, 592.53) Remaining time to fill dewars is 13380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 30 60 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.13 -0.13 (2nd move error 0.12 -0.01) Finished aligning to item in 1 rounds Trimming A by 20 20 70 20, reference by 22 30 60 20 -2.7 0.9 unbinned pixels; 6.7 8.3 nm along two shift axes; -2.8 -10.6 nm on specimen axes Total_Shift = 5.63913 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 14.94, -8.34 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -1.6, 6.3 unbinned pixels, 0.12 nm/sec Measured defocus = -0.53 microns changed by -1.07 to target 34 frames are being saved to U:\krios\20190806\slot11_1274.tif 34 frames are being saved to U:\krios\20190806\slot11_1275.tif 34 frames are being saved to U:\krios\20190806\slot11_1276.tif Item 495: Stage: 59.60 592.53 83 Label: 493 495 Tick time from program start = 75706.021 Running script 17 at item 494 (60.63, 590.23) Remaining time to fill dewars is 13320 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 24 65 110 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.19 -0.11 (2nd move error 0.06 0.01) Finished aligning to item in 1 rounds Trimming A by 23 65 110 20, reference by 24 65 110 20 0.6 -0.5 unbinned pixels; 2.4 1.3 nm along two shift axes; 1.7 2.3 nm on specimen axes Total_Shift = 1.444598 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 18.66, -10.45 unbinned pixels, unfiltered CCC is 0.0009 The average drift between shots was -5.9, 5.4 unbinned pixels, 0.14 nm/sec Measured defocus = -0.66 microns changed by -1.04 to target 34 frames are being saved to U:\krios\20190806\slot11_1277.tif 34 frames are being saved to U:\krios\20190806\slot11_1278.tif 34 frames are being saved to U:\krios\20190806\slot11_1279.tif Item 496: Stage: 60.63 590.23 83 Label: 494 496 Tick time from program start = 75818.561 Running script 17 at item 495 (58.39, 589.20) Remaining time to fill dewars is 13140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 60 60 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.13 -0.14 (2nd move error 0.12 -0.03) Finished aligning to item in 1 rounds Trimming A by 24 65 60 20, reference by 20 60 60 20 -4.5 -3.2 unbinned pixels; 5.8 20.9 nm along two shift axes; 13.3 -16.8 nm on specimen axes Total_Shift = 10.996486 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 23.70, -13.90 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -0.9, 4.3 unbinned pixels, 0.08 nm/sec Measured defocus = -0.85 microns changed by -0.95 to target 34 frames are being saved to U:\krios\20190806\slot11_1280.tif 34 frames are being saved to U:\krios\20190806\slot11_1281.tif 34 frames are being saved to U:\krios\20190806\slot11_1282.tif Item 497: Stage: 58.39 589.20 83 Label: 495 497 Tick time from program start = 75935.156 Running script 17 at item 496 (57.34, 591.50) Remaining time to fill dewars is 12960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.18 -0.17 (2nd move error 0.07 -0.06) Finished aligning to item in 1 rounds -7.9 -3.2 unbinned pixels; 1.4 33.4 nm along two shift axes; 14.2 -30.2 nm on specimen axes Total_Shift = 17.129579 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 26.30, -13.38 unbinned pixels, unfiltered CCC is 0.0022 The average drift between shots was -1.3, 5.1 unbinned pixels, 0.09 nm/sec Measured defocus = -0.91 microns changed by -0.99 to target 34 frames are being saved to U:\krios\20190806\slot11_1283.tif 34 frames are being saved to U:\krios\20190806\slot11_1284.tif 34 frames are being saved to U:\krios\20190806\slot11_1285.tif Item 498: Stage: 57.34 591.50 83 Label: 496 498 Tick time from program start = 76048.568 Running script 17 at item 497 (56.28, 593.82) Remaining time to fill dewars is 12960 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.17 -0.18 (2nd move error 0.08 -0.07) Finished aligning to item in 1 rounds -9.5 -0.0 unbinned pixels; 12.2 34.3 nm along two shift axes; 2.2 -37.0 nm on specimen axes Total_Shift = 19.034819 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 28.85, -15.90 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -0.6, 3.2 unbinned pixels, 0.06 nm/sec Measured defocus = -1.02 microns changed by -0.98 to target 34 frames are being saved to U:\krios\20190806\slot11_1286.tif 34 frames are being saved to U:\krios\20190806\slot11_1287.tif 34 frames are being saved to U:\krios\20190806\slot11_1288.tif Item 499: Stage: 56.28 593.82 83 Label: 497 499 Tick time from program start = 76161.919 Running script 17 at item 498 (54.13, 598.47) Remaining time to fill dewars is 12720 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 158 20 20 Trimming A by 20 20 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.19 -0.13 Disparity in stage position after aligning to target position: -0.29 -0.20 (2nd move error -0.10 -0.10) Finished aligning to item in 2 rounds -13.1 20.0 unbinned pixels; 90.8 17.1 nm along two shift axes; -75.2 -55.3 nm on specimen axes Total_Shift = 47.916554 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 33.05, -18.06 unbinned pixels, unfiltered CCC is 0.0031 The average drift between shots was -3.5, -0.5 unbinned pixels, 0.06 nm/sec Measured defocus = -1.16 microns changed by -0.94 to target 34 frames are being saved to U:\krios\20190806\slot11_1289.tif 34 frames are being saved to U:\krios\20190806\slot11_1290.tif 34 frames are being saved to U:\krios\20190806\slot11_1291.tif Item 500: Stage: 54.13 598.47 83 Label: 498 500 Tick time from program start = 76285.019 Running script 17 at item 499 (51.86, 603.29) Remaining time to fill dewars is 12600 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.19 -0.14 Disparity in stage position after aligning to target position: -0.13 -0.26 (2nd move error 0.06 -0.15) Finished aligning to item in 2 rounds -18.8 -2.7 unbinned pixels; 14.7 71.8 nm along two shift axes; 14.4 -72.7 nm on specimen axes Total_Shift = 38.044079 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 41.33, -25.26 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was 1.1, 5.6 unbinned pixels, 0.10 nm/sec Measured defocus = -1.50 microns changed by -0.70 to target 34 frames are being saved to U:\krios\20190806\slot11_1292.tif 34 frames are being saved to U:\krios\20190806\slot11_1293.tif 34 frames are being saved to U:\krios\20190806\slot11_1294.tif Item 501: Stage: 51.86 603.29 83 Label: 499 501 Tick time from program start = 76406.216 Running script 17 at item 500 (50.76, 605.45) Remaining time to fill dewars is 12420 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.32 -0.17 Disparity in stage position after aligning to target position: -0.09 -0.08 (2nd move error 0.23 0.07) Finished aligning to item in 2 rounds -1.1 -4.7 unbinned pixels; 16.0 11.3 nm along two shift axes; 18.7 -3.4 nm on specimen axes Total_Shift = 9.761687 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 41.42, -26.11 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -4.1, 8.2 unbinned pixels, 0.16 nm/sec Measured defocus = -1.51 microns changed by -0.79 to target 34 frames are being saved to U:\krios\20190806\slot11_1295.tif 34 frames are being saved to U:\krios\20190806\slot11_1296.tif 34 frames are being saved to U:\krios\20190806\slot11_1297.tif Item 502: Stage: 50.76 605.45 83 Label: 500 502 Tick time from program start = 76528.911 Running script 17 at item 501 (49.67, 607.72) Remaining time to fill dewars is 12240 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.19 -0.14 (2nd move error 0.14 0.00) Finished aligning to item in 1 rounds 0.5 -3.5 unbinned pixels; 13.5 3.6 nm along two shift axes; 13.6 2.6 nm on specimen axes Total_Shift = 7.087843 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 44.13, -27.03 unbinned pixels, unfiltered CCC is -0.0009 The average drift between shots was -1.9, 6.6 unbinned pixels, 0.12 nm/sec Measured defocus = -1.60 microns changed by -0.80 to target 34 frames are being saved to U:\krios\20190806\slot11_1298.tif 34 frames are being saved to U:\krios\20190806\slot11_1299.tif 34 frames are being saved to U:\krios\20190806\slot11_1300.tif Item 503: Stage: 49.67 607.72 83 Label: 501 503 Tick time from program start = 76642.152 Running script 17 at item 502 (48.62, 610.05) Remaining time to fill dewars is 12180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.16 -0.10 (2nd move error 0.16 0.04) Finished aligning to item in 1 rounds 0.3 3.3 unbinned pixels; 11.7 5.8 nm along two shift axes; -12.8 0.3 nm on specimen axes Total_Shift = 6.554856 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 42.03, -24.60 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -2.6, 2.4 unbinned pixels, 0.06 nm/sec Measured defocus = -1.51 microns changed by -0.99 to target 34 frames are being saved to U:\krios\20190806\slot11_1301.tif 34 frames are being saved to U:\krios\20190806\slot11_1302.tif 34 frames are being saved to U:\krios\20190806\slot11_1303.tif Item 504: Stage: 48.62 610.05 83 Label: 502 504 Tick time from program start = 76755.721 Running script 17 at item 503 (47.61, 612.39) Remaining time to fill dewars is 12000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.21 -0.15 (2nd move error 0.12 -0.00) Finished aligning to item in 1 rounds -1.4 -5.4 unbinned pixels; 18.2 13.4 nm along two shift axes; 21.5 -4.4 nm on specimen axes Total_Shift = 11.265679 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 42.27, -24.55 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 0.0, 4.8 unbinned pixels, 0.08 nm/sec Measured defocus = -1.51 microns changed by 0.21 to target 34 frames are being saved to U:\krios\20190806\slot11_1304.tif 34 frames are being saved to U:\krios\20190806\slot11_1305.tif 34 frames are being saved to U:\krios\20190806\slot11_1306.tif Item 505: Stage: 47.61 612.39 83 Label: 503 505 Tick time from program start = 76869.352 Running script 17 at item 504 (46.55, 614.74) Remaining time to fill dewars is 11880 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.28 -0.12 (2nd move error 0.04 0.02) Finished aligning to item in 1 rounds -0.8 -3.1 unbinned pixels; 10.2 7.3 nm along two shift axes; 12.0 -2.3 nm on specimen axes Total_Shift = 6.290355 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 7.69, -0.82 unbinned pixels, unfiltered CCC is 0.0011 The average drift between shots was -6.5, 2.7 unbinned pixels, 0.13 nm/sec Measured defocus = -0.22 microns changed by -1.18 to target 34 frames are being saved to U:\krios\20190806\slot11_1307.tif 34 frames are being saved to U:\krios\20190806\slot11_1308.tif 34 frames are being saved to U:\krios\20190806\slot11_1309.tif Item 506: Stage: 46.55 614.74 83 Label: 504 506 Tick time from program start = 76982.359 Running script 17 at item 505 (45.30, 617.07) Remaining time to fill dewars is 11760 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.16 Disparity in stage position after aligning to target position: -0.14 -0.19 (2nd move error 0.15 -0.05) Finished aligning to item in 2 rounds -6.2 -3.6 unbinned pixels; 5.1 27.5 nm along two shift axes; 15.2 -23.2 nm on specimen axes Total_Shift = 14.239536 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 6.29, -0.93 unbinned pixels, unfiltered CCC is 0.0037 The average drift between shots was 0.1, 2.7 unbinned pixels, 0.05 nm/sec Measured defocus = -0.18 microns changed by -1.32 to target 34 frames are being saved to U:\krios\20190806\slot11_1310.tif 34 frames are being saved to U:\krios\20190806\slot11_1311.tif 34 frames are being saved to U:\krios\20190806\slot11_1312.tif Item 507: Stage: 45.30 617.07 83 Label: 505 507 Tick time from program start = 77104.820 Running script 17 at item 506 (44.26, 619.37) Remaining time to fill dewars is 11640 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.20 -0.17 (2nd move error 0.09 -0.03) Finished aligning to item in 1 rounds -3.2 0.8 unbinned pixels; 7.0 10.5 nm along two shift axes; -2.3 -12.8 nm on specimen axes Total_Shift = 6.665444 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 8.53, -1.91 unbinned pixels, unfiltered CCC is 0.0209 The average drift between shots was -0.9, 2.3 unbinned pixels, 0.04 nm/sec Measured defocus = -0.26 microns changed by -1.34 to target 34 frames are being saved to U:\krios\20190806\slot11_1313.tif 34 frames are being saved to U:\krios\20190806\slot11_1314.tif 34 frames are being saved to U:\krios\20190806\slot11_1315.tif Item 508: Stage: 44.26 619.37 83 Label: 506 508 Tick time from program start = 77218.233 Running script 17 at item 507 (43.22, 621.66) Remaining time to fill dewars is 11580 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.24 -0.16 (2nd move error 0.05 -0.02) Finished aligning to item in 1 rounds -4.2 -3.6 unbinned pixels; 7.7 20.6 nm along two shift axes; 14.9 -15.7 nm on specimen axes Total_Shift = 11.109218 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 9.69, -5.29 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was 1.7, 2.8 unbinned pixels, 0.06 nm/sec Measured defocus = -0.34 microns changed by -1.36 to target 34 frames are being saved to U:\krios\20190806\slot11_1316.tif 34 frames are being saved to U:\krios\20190806\slot11_1317.tif 34 frames are being saved to U:\krios\20190806\slot11_1318.tif Item 509: Stage: 43.22 621.66 83 Label: 507 509 Tick time from program start = 77331.224 Running script 17 at item 508 (42.13, 624.02) Remaining time to fill dewars is 11460 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 50 20 20 25, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.39 -0.14 (2nd move error -0.10 -0.00) Finished aligning to item in 1 rounds Trimming A by 50 20 20 30, reference by 50 20 20 25 -0.8 19.2 unbinned pixels; 71.8 25.9 nm along two shift axes; -74.6 -7.4 nm on specimen axes Total_Shift = 38.504568 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 12.08, -6.01 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -5.9, -2.1 unbinned pixels, 0.11 nm/sec Measured defocus = -0.42 microns changed by -1.38 to target 34 frames are being saved to U:\krios\20190806\slot11_1319.tif 34 frames are being saved to U:\krios\20190806\slot11_1320.tif 34 frames are being saved to U:\krios\20190806\slot11_1321.tif Item 510: Stage: 42.13 624.02 83 Label: 508 510 Tick time from program start = 77444.341 Running script 17 at item 509 (39.88, 622.99) Remaining time to fill dewars is 11280 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 26 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.42 -0.18 (2nd move error -0.13 -0.05) Finished aligning to item in 1 rounds Trimming A by 26 20 20 20, reference by 26 20 20 20 -9.2 40.2 unbinned pixels; 159.9 27.5 nm along two shift axes; -154.6 -44.2 nm on specimen axes Total_Shift = 82.526923 Trimming A by 26 20 20 20, reference by 26 20 20 20 -4.1 30.0 unbinned pixels; 115.5 30.5 nm along two shift axes; -115.7 -22.1 nm on specimen axes Total_Shift = 60.489667 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 17.92, -10.15 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -2.9, 0.5 unbinned pixels, 0.05 nm/sec Measured defocus = -0.64 microns changed by -1.26 to target 34 frames are being saved to U:\krios\20190806\slot11_1322.tif 34 frames are being saved to U:\krios\20190806\slot11_1323.tif 34 frames are being saved to U:\krios\20190806\slot11_1324.tif Item 511: Stage: 39.88 622.99 83 Label: 509 511 Tick time from program start = 77563.978 Running script 17 at item 510 (40.97, 620.63) Remaining time to fill dewars is 11100 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.23 -0.21 (2nd move error 0.06 -0.07) Finished aligning to item in 1 rounds -9.4 -2.5 unbinned pixels; 3.1 37.6 nm along two shift axes; 11.7 -36.0 nm on specimen axes Total_Shift = 19.4453 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 25.57, -16.39 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -0.1, 6.4 unbinned pixels, 0.11 nm/sec Measured defocus = -0.94 microns changed by -1.06 to target 34 frames are being saved to U:\krios\20190806\slot11_1325.tif 34 frames are being saved to U:\krios\20190806\slot11_1326.tif 34 frames are being saved to U:\krios\20190806\slot11_1327.tif Item 512: Stage: 40.97 620.63 83 Label: 510 512 Tick time from program start = 77677.032 Running script 17 at item 511 (42.03, 618.33) Remaining time to fill dewars is 10980 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.27 -0.19 (2nd move error 0.02 -0.05) Finished aligning to item in 1 rounds -7.5 -2.1 unbinned pixels; 2.0 30.2 nm along two shift axes; 9.8 -28.8 nm on specimen axes Total_Shift = 15.609485 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 26.75, -15.46 unbinned pixels, unfiltered CCC is 0.0015 The average drift between shots was 0.6, 4.5 unbinned pixels, 0.08 nm/sec Measured defocus = -0.95 microns changed by -1.15 to target 34 frames are being saved to U:\krios\20190806\slot11_1328.tif 34 frames are being saved to U:\krios\20190806\slot11_1329.tif 34 frames are being saved to U:\krios\20190806\slot11_1330.tif Item 513: Stage: 42.03 618.33 83 Label: 511 513 Tick time from program start = 77790.039 Running script 17 at item 512 (44.32, 613.69) Remaining time to fill dewars is 10860 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.20 -0.15 Disparity in stage position after aligning to target position: -0.30 -0.16 (2nd move error -0.09 -0.03) Finished aligning to item in 2 rounds -4.2 17.8 unbinned pixels; 71.1 11.7 nm along two shift axes; -68.5 -20.1 nm on specimen axes Total_Shift = 36.641396 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 69.18, -44.74 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -0.1, 3.3 unbinned pixels, 0.06 nm/sec Measured defocus = -2.55 microns changed by 0.35 to target 34 frames are being saved to U:\krios\20190806\slot11_1331.tif 34 frames are being saved to U:\krios\20190806\slot11_1332.tif 34 frames are being saved to U:\krios\20190806\slot11_1333.tif Item 514: Stage: 44.32 613.69 83 Label: 512 514 Tick time from program start = 77912.156 Running script 17 at item 513 (45.39, 611.32) Remaining time to fill dewars is 10740 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.20 -0.13 (2nd move error -0.00 0.00) Finished aligning to item in 1 rounds -1.0 0.4 unbinned pixels; 2.8 3.0 nm along two shift axes; -1.4 -4.0 nm on specimen axes Total_Shift = 2.172381 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 40.68, -26.69 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -4.6, 4.9 unbinned pixels, 0.12 nm/sec Measured defocus = -1.50 microns changed by -0.80 to target 34 frames are being saved to U:\krios\20190806\slot11_1334.tif 34 frames are being saved to U:\krios\20190806\slot11_1335.tif 34 frames are being saved to U:\krios\20190806\slot11_1336.tif Item 515: Stage: 45.39 611.32 83 Label: 513 515 Tick time from program start = 78022.480 Running script 17 at item 514 (46.41, 608.96) Remaining time to fill dewars is 10680 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.19 -0.12 (2nd move error 0.01 0.01) Finished aligning to item in 1 rounds 1.9 -1.3 unbinned pixels; 7.2 4.8 nm along two shift axes; 4.7 7.6 nm on specimen axes Total_Shift = 4.565035 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 46.20, -28.67 unbinned pixels, unfiltered CCC is 0.0000 The average drift between shots was -2.9, 4.3 unbinned pixels, 0.09 nm/sec Measured defocus = -1.68 microns changed by -0.72 to target 34 frames are being saved to U:\krios\20190806\slot11_1337.tif 34 frames are being saved to U:\krios\20190806\slot11_1338.tif 34 frames are being saved to U:\krios\20190806\slot11_1339.tif Item 516: Stage: 46.41 608.96 83 Label: 514 516 Tick time from program start = 78138.030 Running script 17 at item 515 (50.87, 599.60) Remaining time to fill dewars is 10560 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 150 20 65 Trimming A by 20 20 20 20, reference by 20 158 20 20 Trimming A by 20 20 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.19 Disparity in stage position after aligning to target position: -0.21 -0.03 (2nd move error 0.03 0.13) Finished aligning to item in 2 rounds 1.5 -0.8 unbinned pixels; 4.9 4.2 nm along two shift axes; 2.8 6.0 nm on specimen axes Total_Shift = 3.408051 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 49.12, -29.25 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -7.5, 9.0 unbinned pixels, 0.21 nm/sec Measured defocus = -1.77 microns changed by -0.73 to target 34 frames are being saved to U:\krios\20190806\slot11_1340.tif 34 frames are being saved to U:\krios\20190806\slot11_1341.tif 34 frames are being saved to U:\krios\20190806\slot11_1342.tif Item 517: Stage: 50.87 599.60 83 Label: 515 517 Tick time from program start = 78258.759 Running script 17 at item 516 (53.02, 595.08) Remaining time to fill dewars is 10380 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 158 20 20 Trimming A by 20 20 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.26 -0.09 Disparity in stage position after aligning to target position: -0.29 -0.25 (2nd move error -0.03 -0.18) Finished aligning to item in 2 rounds -26.5 21.3 unbinned pixels; 113.0 63.2 nm along two shift axes; -77.4 -107.5 nm on specimen axes Total_Shift = 67.995574 -10.8 15.8 unbinned pixels; 72.3 15.1 nm along two shift axes; -59.3 -45.4 nm on specimen axes Total_Shift = 38.330516 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 45.17, -28.06 unbinned pixels, unfiltered CCC is 0.0010 The average drift between shots was -0.7, 1.6 unbinned pixels, 0.03 nm/sec Measured defocus = -1.64 microns changed by 0.34 to target 34 frames are being saved to U:\krios\20190806\slot11_1343.tif 34 frames are being saved to U:\krios\20190806\slot11_1344.tif 34 frames are being saved to U:\krios\20190806\slot11_1345.tif Item 518: Stage: 53.02 595.08 83 Label: 516 518 Tick time from program start = 78385.292 Running script 17 at item 517 (54.05, 592.75) Remaining time to fill dewars is 10260 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.20 -0.23 (2nd move error 0.06 -0.16) Finished aligning to item in 1 rounds -21.6 -2.5 unbinned pixels; 19.1 81.6 nm along two shift axes; 14.2 -83.6 nm on specimen axes Total_Shift = 43.542966 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 9.72, -4.77 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was 1.2, 5.1 unbinned pixels, 0.09 nm/sec Measured defocus = -0.33 microns changed by -0.97 to target 34 frames are being saved to U:\krios\20190806\slot11_1346.tif 34 frames are being saved to U:\krios\20190806\slot11_1347.tif 34 frames are being saved to U:\krios\20190806\slot11_1348.tif Item 519: Stage: 54.05 592.75 83 Label: 517 519 Tick time from program start = 78498.096 Running script 17 at item 518 (55.15, 590.45) Remaining time to fill dewars is 10020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.12 -0.23 (2nd move error 0.14 -0.16) Finished aligning to item in 1 rounds -17.2 1.2 unbinned pixels; 27.0 60.1 nm along two shift axes; -1.2 -67.3 nm on specimen axes Total_Shift = 34.529914 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 13.31, -7.44 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was 0.8, 4.9 unbinned pixels, 0.09 nm/sec Measured defocus = -0.47 microns changed by -0.93 to target 34 frames are being saved to U:\krios\20190806\slot11_1349.tif 34 frames are being saved to U:\krios\20190806\slot11_1350.tif 34 frames are being saved to U:\krios\20190806\slot11_1351.tif Item 520: Stage: 55.15 590.45 83 Label: 518 520 Tick time from program start = 78611.228 Running script 17 at item 519 (54.08, 587.00) Remaining time to fill dewars is 9960 Dewars are NOT busy filling Trimming A by 20 230 125 20, reference by 20 20 20 20 Trimming A by 20 230 125 20, reference by 20 20 20 20 Trimming A by 20 230 125 20, reference by 20 158 20 20 Trimming A by 20 230 125 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.21 -0.11 Trimming A by 20 50 35 20, reference by 21 225 115 20 Disparity in stage position after aligning to target position: -0.29 -0.25 (2nd move error -0.08 -0.17) Finished aligning to item in 2 rounds Trimming A by 20 50 40 20, reference by 20 50 35 20 -23.0 17.3 unbinned pixels; 93.6 56.9 nm along two shift axes; -62.4 -93.3 nm on specimen axes Total_Shift = 57.599215 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 17.14, -7.65 unbinned pixels, unfiltered CCC is 0.0023 The average drift between shots was -5.4, 1.0 unbinned pixels, 0.10 nm/sec Measured defocus = -0.58 microns changed by -0.92 to target 34 frames are being saved to U:\krios\20190806\slot11_1352.tif 34 frames are being saved to U:\krios\20190806\slot11_1353.tif 34 frames are being saved to U:\krios\20190806\slot11_1354.tif Item 521: Stage: 54.08 587.00 83 Label: 519 521 Tick time from program start = 78734.734 Running script 17 at item 520 (52.83, 589.40) Remaining time to fill dewars is 9840 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.16 -0.24 (2nd move error 0.10 -0.17) Finished aligning to item in 1 rounds -20.2 -4.4 unbinned pixels; 10.3 79.3 nm along two shift axes; 21.3 -77.6 nm on specimen axes Total_Shift = 41.320284 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 21.40, -12.43 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was 1.4, 7.3 unbinned pixels, 0.13 nm/sec Measured defocus = -0.76 microns changed by -0.84 to target 34 frames are being saved to U:\krios\20190806\slot11_1355.tif 34 frames are being saved to U:\krios\20190806\slot11_1356.tif 34 frames are being saved to U:\krios\20190806\slot11_1357.tif Item 522: Stage: 52.83 589.40 83 Label: 520 522 Tick time from program start = 78848.381 Running script 17 at item 521 (51.75, 591.56) Remaining time to fill dewars is 9660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.10 -0.08 (2nd move error 0.15 0.09) Finished aligning to item in 1 rounds 1.8 4.1 unbinned pixels; 12.5 12.7 nm along two shift axes; -16.2 6.3 nm on specimen axes Total_Shift = 8.892911 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 25.30, -14.35 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -7.5, 2.0 unbinned pixels, 0.14 nm/sec Measured defocus = -0.90 microns changed by -0.80 to target 34 frames are being saved to U:\krios\20190806\slot11_1358.tif 34 frames are being saved to U:\krios\20190806\slot11_1359.tif 34 frames are being saved to U:\krios\20190806\slot11_1360.tif Item 523: Stage: 51.75 591.56 83 Label: 521 523 Tick time from program start = 78961.887 Running script 17 at item 522 (41.02, 614.99) Remaining time to fill dewars is 9600 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.14 Disparity in stage position after aligning to target position: -0.39 -0.22 (2nd move error -0.14 -0.10) Finished aligning to item in 2 rounds -14.2 27.9 unbinned pixels; 121.0 9.0 nm along two shift axes; -105.5 -61.0 nm on specimen axes Total_Shift = 62.539389 -6.6 18.9 unbinned pixels; 78.1 4.5 nm along two shift axes; -72.1 -29.8 nm on specimen axes Total_Shift = 40.052376 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 25.90, -13.40 unbinned pixels, unfiltered CCC is -0.0004 The average drift between shots was -3.3, 1.1 unbinned pixels, 0.06 nm/sec Measured defocus = -0.90 microns changed by -0.90 to target 34 frames are being saved to U:\krios\20190806\slot11_1361.tif 34 frames are being saved to U:\krios\20190806\slot11_1362.tif 34 frames are being saved to U:\krios\20190806\slot11_1363.tif Item 524: Stage: 41.02 614.99 83 Label: 522 524 Tick time from program start = 79091.118 Running script 17 at item 523 (39.82, 617.31) Remaining time to fill dewars is 9420 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.13 Disparity in stage position after aligning to target position: -0.37 -0.25 (2nd move error -0.10 -0.14) Finished aligning to item in 2 rounds -19.1 21.3 unbinned pixels; 103.2 36.8 nm along two shift axes; -78.8 -78.8 nm on specimen axes Total_Shift = 57.211185 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 24.24, -14.75 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -2.4, 2.7 unbinned pixels, 0.06 nm/sec Measured defocus = -0.88 microns changed by -1.02 to target 34 frames are being saved to U:\krios\20190806\slot11_1364.tif 34 frames are being saved to U:\krios\20190806\slot11_1365.tif 34 frames are being saved to U:\krios\20190806\slot11_1366.tif Item 525: Stage: 39.82 617.31 83 Label: 523 525 Tick time from program start = 79212.144 Running script 17 at item 524 (38.72, 619.61) Remaining time to fill dewars is 9300 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.38 -0.22 (2nd move error -0.10 -0.11) Finished aligning to item in 1 rounds -14.6 19.1 unbinned pixels; 89.4 23.8 nm along two shift axes; -71.3 -60.8 nm on specimen axes Total_Shift = 48.132879 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 21.82, -16.04 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -0.4, 2.5 unbinned pixels, 0.05 nm/sec Measured defocus = -0.83 microns changed by -1.17 to target 34 frames are being saved to U:\krios\20190806\slot11_1367.tif 34 frames are being saved to U:\krios\20190806\slot11_1368.tif 34 frames are being saved to U:\krios\20190806\slot11_1369.tif Item 526: Stage: 38.72 619.61 83 Label: 524 526 Tick time from program start = 79325.697 Running script 17 at item 525 (37.61, 621.80) Remaining time to fill dewars is 9180 Dewars are NOT busy filling Started a long running operation: running buffer cycle Call for running buffer cycle finished successfully Trimming A by 26 20 20 20, reference by 120 23 20 170 Trimming A by 26 20 20 20, reference by 20 20 20 20 Trimming A by 26 20 20 20, reference by 20 20 20 20 Trimming A by 26 20 20 20, reference by 20 20 20 90 Trimming A by 26 20 20 20, reference by 20 20 20 20 Trimming A by 26 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.14 Disparity in stage position after aligning to target position: -0.22 -0.04 (2nd move error 0.16 0.08) Finished aligning to item in 2 rounds 0.5 -9.2 unbinned pixels; 34.5 12.1 nm along two shift axes; 35.7 3.8 nm on specimen axes Total_Shift = 18.439227 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 19.03, -13.89 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -3.7, 11.5 unbinned pixels, 0.22 nm/sec Measured defocus = -0.73 microns changed by -1.37 to target 34 frames are being saved to U:\krios\20190806\slot11_1370.tif 34 frames are being saved to U:\krios\20190806\slot11_1371.tif 34 frames are being saved to U:\krios\20190806\slot11_1372.tif Item 527: Stage: 37.61 621.80 83 Label: 525 527 Tick time from program start = 79466.582 Running script 17 at item 526 (34.32, 623.04) Remaining time to fill dewars is 9180 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 45 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.29 -0.11 (2nd move error 0.09 0.01) Finished aligning to item in 1 rounds Trimming A by 45 20 20 20, reference by 45 20 20 20 0.5 -3.0 unbinned pixels; 11.6 2.6 nm along two shift axes; 11.5 2.6 nm on specimen axes Total_Shift = 6.053784 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 53.24, -36.38 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -1.3, 9.7 unbinned pixels, 0.17 nm/sec Measured defocus = -1.99 microns changed by -0.21 to target 34 frames are being saved to U:\krios\20190806\slot11_1373.tif 34 frames are being saved to U:\krios\20190806\slot11_1374.tif 34 frames are being saved to U:\krios\20190806\slot11_1375.tif Item 528: Stage: 34.32 623.04 83 Label: 526 528 Tick time from program start = 79580.493 Running script 17 at item 527 (35.40, 620.69) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.21 -0.15 (2nd move error 0.17 -0.02) Finished aligning to item in 1 rounds -1.5 4.2 unbinned pixels; 17.3 0.9 nm along two shift axes; -15.9 -6.7 nm on specimen axes Total_Shift = 8.85926 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 24.86, -16.45 unbinned pixels, unfiltered CCC is 0.0019 The average drift between shots was 0.5, 6.5 unbinned pixels, 0.12 nm/sec Measured defocus = -0.92 microns changed by -1.38 to target 34 frames are being saved to U:\krios\20190806\slot11_1376.tif 34 frames are being saved to U:\krios\20190806\slot11_1377.tif 34 frames are being saved to U:\krios\20190806\slot11_1378.tif Item 529: Stage: 35.40 620.69 83 Label: 527 529 Tick time from program start = 79693.563 Running script 17 at item 528 (36.43, 618.40) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.32 -0.13 (2nd move error 0.07 -0.01) Finished aligning to item in 1 rounds -2.3 -2.7 unbinned pixels; 6.8 12.5 nm along two shift axes; 10.9 -8.5 nm on specimen axes Total_Shift = 7.11875 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 64.25, -43.00 unbinned pixels, unfiltered CCC is -0.0013 The average drift between shots was 2.0, 8.7 unbinned pixels, 0.16 nm/sec Measured defocus = -2.39 microns changed by -0.01 to target 34 frames are being saved to U:\krios\20190806\slot11_1379.tif 34 frames are being saved to U:\krios\20190806\slot11_1380.tif 34 frames are being saved to U:\krios\20190806\slot11_1381.tif Item 530: Stage: 36.43 618.40 83 Label: 528 530 Tick time from program start = 79806.804 Running script 17 at item 529 (37.59, 616.17) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.10 -0.11 (2nd move error 0.27 0.01) Finished aligning to item in 1 rounds 3.0 -15.2 unbinned pixels; 59.8 11.9 nm along two shift axes; 58.4 15.0 nm on specimen axes Total_Shift = 30.945814 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 38.55, -25.41 unbinned pixels, unfiltered CCC is -0.0011 The average drift between shots was -3.2, 12.0 unbinned pixels, 0.22 nm/sec Measured defocus = -1.43 microns changed by -1.07 to target 34 frames are being saved to U:\krios\20190806\slot11_1382.tif 34 frames are being saved to U:\krios\20190806\slot11_1383.tif 34 frames are being saved to U:\krios\20190806\slot11_1384.tif Item 531: Stage: 37.59 616.17 83 Label: 529 531 Tick time from program start = 79920.357 Running script 17 at item 530 (38.71, 613.93) Remaining time to fill dewars is 9060 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 75 24 70 20 Disparity in stage position after aligning to center of map frame: -0.22 -0.12 Disparity in stage position after aligning to target position: -0.45 -0.27 (2nd move error -0.23 -0.17) Finished aligning to item in 2 rounds -19.5 40.6 unbinned pixels; 174.8 9.1 nm along two shift axes; -153.9 -84.4 nm on specimen axes Total_Shift = 90.115352 -2.2 25.1 unbinned pixels; 95.2 29.9 nm along two shift axes; -97.3 -13.9 nm on specimen axes Total_Shift = 50.43249 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 44.25, -28.29 unbinned pixels, unfiltered CCC is -0.0013 The average drift between shots was 2.7, 3.6 unbinned pixels, 0.08 nm/sec Measured defocus = -1.62 microns changed by 0.32 to target 34 frames are being saved to U:\krios\20190806\slot11_1385.tif 34 frames are being saved to U:\krios\20190806\slot11_1386.tif 34 frames are being saved to U:\krios\20190806\slot11_1387.tif Item 532: Stage: 38.71 613.93 83 Label: 530 532 Tick time from program start = 80048.886 Running script 17 at item 531 (49.51, 590.43) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 150 20 65 Trimming A by 20 20 20 20, reference by 20 158 20 20 Trimming A by 20 20 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.18 Disparity in stage position after aligning to target position: -0.16 -0.12 (2nd move error 0.11 0.04) Finished aligning to item in 2 rounds -1.3 -3.0 unbinned pixels; 9.2 9.2 nm along two shift axes; 11.9 -4.5 nm on specimen axes Total_Shift = 6.507727 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 12.15, -7.68 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -6.6, 8.8 unbinned pixels, 0.19 nm/sec Measured defocus = -0.44 microns changed by -0.96 to target 34 frames are being saved to U:\krios\20190806\slot11_1388.tif 34 frames are being saved to U:\krios\20190806\slot11_1389.tif 34 frames are being saved to U:\krios\20190806\slot11_1390.tif Item 533: Stage: 49.51 590.43 83 Label: 531 533 Tick time from program start = 80172.751 Running script 17 at item 532 (50.67, 588.01) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Ending Navigator acquisition after current item Trimming A by 20 160 20 20, reference by 20 20 20 20 Trimming A by 20 160 20 20, reference by 20 20 20 20 Trimming A by 20 160 20 20, reference by 20 20 20 20 Trimming A by 20 160 20 20, reference by 20 150 20 65 Trimming A by 20 160 20 20, reference by 20 158 20 20 Trimming A by 20 160 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.13 -0.16 Trimming A by 20 20 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.08 -0.01 (2nd move error 0.05 0.14) Finished aligning to item in 2 rounds -1.9 -0.5 unbinned pixels; 0.7 7.5 nm along two shift axes; 2.3 -7.2 nm on specimen axes Total_Shift = 3.883019 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 16.03, -8.05 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -8.9, 6.5 unbinned pixels, 0.20 nm/sec Measured defocus = -0.55 microns changed by -0.95 to target 34 frames are being saved to U:\krios\20190806\slot11_1391.tif 34 frames are being saved to U:\krios\20190806\slot11_1392.tif 34 frames are being saved to U:\krios\20190806\slot11_1393.tif Item 534: Stage: 50.67 588.01 83 Label: 532 534 Tick time from program start = 80294.728 Running script 17 at item 533 (51.71, 585.69) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 70 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.09 -0.02 (2nd move error 0.03 0.13) Finished aligning to item in 1 rounds Trimming A by 20 60 35 20, reference by 20 70 20 20 -2.5 -0.8 unbinned pixels; 0.2 10.1 nm along two shift axes; 3.7 -9.4 nm on specimen axes Total_Shift = 5.177617 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 17.73, -7.71 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -7.5, 6.1 unbinned pixels, 0.17 nm/sec Measured defocus = -0.59 microns changed by -1.01 to target 34 frames are being saved to U:\krios\20190806\slot11_1394.tif 34 frames are being saved to U:\krios\20190806\slot11_1395.tif 34 frames are being saved to U:\krios\20190806\slot11_1396.tif Item 535: Stage: 51.71 585.69 83 Label: 533 535 Tick time from program start = 80452.929 Running script 17 at item 534 (49.47, 584.60) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 90 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.08 -0.05 (2nd move error 0.05 0.09) Finished aligning to item in 1 rounds Trimming A by 20 89 20 20, reference by 20 90 20 20 -0.5 -3.0 unbinned pixels; 10.4 6.2 nm along two shift axes; 11.7 -1.2 nm on specimen axes Total_Shift = 6.041836 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 18.62, -10.67 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was -7.2, 6.1 unbinned pixels, 0.17 nm/sec Measured defocus = -0.66 microns changed by -1.04 to target 34 frames are being saved to U:\krios\20190806\slot11_1397.tif 34 frames are being saved to U:\krios\20190806\slot11_1398.tif 34 frames are being saved to U:\krios\20190806\slot11_1399.tif Item 536: Stage: 49.47 584.60 83 Label: 534 536 Tick time from program start = 80565.983 Running script 17 at item 535 (48.43, 586.95) Remaining time to fill dewars is 9000 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.05 -0.07 (2nd move error 0.07 0.08) Finished aligning to item in 1 rounds -1.0 0.3 unbinned pixels; 2.5 3.2 nm along two shift axes; -1.0 -4.0 nm on specimen axes Total_Shift = 2.131731 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 23.72, -12.75 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -9.0, 7.1 unbinned pixels, 0.20 nm/sec Measured defocus = -0.83 microns changed by -0.97 to target 34 frames are being saved to U:\krios\20190806\slot11_1400.tif 34 frames are being saved to U:\krios\20190806\slot11_1401.tif 34 frames are being saved to U:\krios\20190806\slot11_1402.tif Item 537: Stage: 48.43 586.95 83 Label: 535 537 Tick time from program start = 80678.850 Running script 17 at item 536 (47.32, 589.41) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.10 -0.11 (2nd move error 0.18 0.04) Finished aligning to item in 1 rounds -0.9 -6.8 unbinned pixels; 24.0 13.6 nm along two shift axes; 26.8 -2.2 nm on specimen axes Total_Shift = 13.821224 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 24.33, -15.65 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -4.9, 8.0 unbinned pixels, 0.17 nm/sec Measured defocus = -0.89 microns changed by -1.01 to target 34 frames are being saved to U:\krios\20190806\slot11_1403.tif 34 frames are being saved to U:\krios\20190806\slot11_1404.tif 34 frames are being saved to U:\krios\20190806\slot11_1405.tif Item 538: Stage: 47.32 589.41 83 Label: 536 538 Tick time from program start = 80792.075 Running script 17 at item 537 (36.54, 612.59) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 120 23 20 170 Trimming A by 20 20 20 20, reference by 20 20 20 90 Trimming A by 20 20 20 20, reference by 20 20 20 110 Disparity in stage position after aligning to center of map frame: -0.37 -0.16 Disparity in stage position after aligning to target position: -0.45 -0.04 (2nd move error -0.09 0.10) Finished aligning to item in 2 rounds 0.3 24.2 unbinned pixels; 88.6 37.5 nm along two shift axes; -94.2 -3.9 nm on specimen axes Total_Shift = 48.391074 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 27.90, -15.99 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was -8.0, 1.9 unbinned pixels, 0.15 nm/sec Measured defocus = -0.99 microns changed by -1.01 to target 34 frames are being saved to U:\krios\20190806\slot11_1406.tif 34 frames are being saved to U:\krios\20190806\slot11_1407.tif 34 frames are being saved to U:\krios\20190806\slot11_1408.tif Item 539: Stage: 36.54 612.59 83 Label: 537 539 Tick time from program start = 80913.538 Running script 17 at item 538 (35.41, 614.99) Remaining time to fill dewars is 8940 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.33 -0.09 (2nd move error 0.03 0.05) Finished aligning to item in 1 rounds -3.5 -1.9 unbinned pixels; 2.5 15.5 nm along two shift axes; 8.2 -13.3 nm on specimen axes Total_Shift = 8.014766 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 27.05, -16.65 unbinned pixels, unfiltered CCC is 0.0004 The average drift between shots was -1.8, 5.6 unbinned pixels, 0.11 nm/sec Measured defocus = -0.98 microns changed by -1.12 to target 34 frames are being saved to U:\krios\20190806\slot11_1409.tif 34 frames are being saved to U:\krios\20190806\slot11_1410.tif 34 frames are being saved to U:\krios\20190806\slot11_1411.tif Item 540: Stage: 35.41 614.99 83 Label: 538 540 Tick time from program start = 81026.498 Running script 17 at item 539 (34.20, 617.28) Remaining time to fill dewars is 7800 Dewars are NOT busy filling Trimming A by 26 20 20 20, reference by 120 23 20 170 Trimming A by 26 20 20 20, reference by 20 20 20 20 Trimming A by 26 20 20 20, reference by 20 20 20 20 Trimming A by 26 20 20 20, reference by 20 20 20 90 Trimming A by 26 20 20 20, reference by 20 20 20 20 Trimming A by 26 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.32 -0.16 Disparity in stage position after aligning to target position: -0.16 -0.13 (2nd move error 0.16 0.01) Finished aligning to item in 2 rounds 2.3 -4.0 unbinned pixels; 17.7 2.3 nm along two shift axes; 15.1 9.8 nm on specimen axes Total_Shift = 9.241681 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 26.12, -14.36 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -4.4, 7.7 unbinned pixels, 0.16 nm/sec Measured defocus = -0.92 microns changed by -1.28 to target 34 frames are being saved to U:\krios\20190806\slot11_1412.tif 34 frames are being saved to U:\krios\20190806\slot11_1413.tif 34 frames are being saved to U:\krios\20190806\slot11_1414.tif Item 541: Stage: 34.20 617.28 83 Label: 539 541 Tick time from program start = 81149.458 Running script 17 at item 540 (33.17, 619.60) Remaining time to fill dewars is 7620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.27 -0.15 (2nd move error 0.05 -0.01) Finished aligning to item in 1 rounds -3.7 -3.6 unbinned pixels; 8.5 18.9 nm along two shift axes; 14.9 -13.8 nm on specimen axes Total_Shift = 10.41242 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 60.69, -40.42 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was 3.2, 10.4 unbinned pixels, 0.19 nm/sec Measured defocus = -2.25 microns changed by -0.05 to target 34 frames are being saved to U:\krios\20190806\slot11_1415.tif 34 frames are being saved to U:\krios\20190806\slot11_1416.tif 34 frames are being saved to U:\krios\20190806\slot11_1417.tif Item 542: Stage: 33.17 619.60 83 Label: 540 542 Tick time from program start = 81262.465 Running script 17 at item 541 (32.14, 621.92) Remaining time to fill dewars is 7500 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.23 -0.14 (2nd move error 0.08 -0.01) Finished aligning to item in 1 rounds -2.1 -1.1 unbinned pixels; 1.3 9.4 nm along two shift axes; 4.8 -8.1 nm on specimen axes Total_Shift = 4.84611 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 25.99, -14.87 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -4.3, 6.6 unbinned pixels, 0.14 nm/sec Measured defocus = -0.93 microns changed by -1.47 to target 34 frames are being saved to U:\krios\20190806\slot11_1418.tif 34 frames are being saved to U:\krios\20190806\slot11_1419.tif 34 frames are being saved to U:\krios\20190806\slot11_1420.tif Item 543: Stage: 32.14 621.92 83 Label: 541 543 Tick time from program start = 81378.499 Running script 17 at item 542 (29.88, 620.86) Remaining time to fill dewars is 7380 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.31 -0.19 (2nd move error 0.01 -0.06) Finished aligning to item in 1 rounds -8.6 -0.4 unbinned pixels; 9.8 31.7 nm along two shift axes; 3.4 -33.5 nm on specimen axes Total_Shift = 17.306043 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 63.24, -42.63 unbinned pixels, unfiltered CCC is -0.0001 The average drift between shots was 2.4, 5.5 unbinned pixels, 0.11 nm/sec Measured defocus = -2.36 microns changed by -0.14 to target 34 frames are being saved to U:\krios\20190806\slot11_1421.tif 34 frames are being saved to U:\krios\20190806\slot11_1422.tif 34 frames are being saved to U:\krios\20190806\slot11_1423.tif Item 544: Stage: 29.88 620.86 83 Label: 542 544 Tick time from program start = 81491.459 Running script 17 at item 543 (30.95, 618.52) Remaining time to fill dewars is 7200 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.23 -0.17 (2nd move error 0.09 -0.04) Finished aligning to item in 1 rounds -5.0 -4.3 unbinned pixels; 9.3 24.5 nm along two shift axes; 17.8 -18.5 nm on specimen axes Total_Shift = 13.194842 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is -0.74, 2.11 unbinned pixels, unfiltered CCC is -0.0096 The average drift between shots was 0.8, 5.1 unbinned pixels, 0.09 nm/sec Measured defocus = 0.05 microns changed by -1.35 to target 34 frames are being saved to U:\krios\20190806\slot11_1424.tif 34 frames are being saved to U:\krios\20190806\slot11_1425.tif 34 frames are being saved to U:\krios\20190806\slot11_1426.tif Item 545: Stage: 30.95 618.52 83 Label: 543 545 Tick time from program start = 81604.606 Running script 17 at item 544 (31.98, 616.25) Remaining time to fill dewars is 7140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.11 -0.20 (2nd move error 0.21 -0.06) Finished aligning to item in 1 rounds -5.4 -0.6 unbinned pixels; 4.7 20.4 nm along two shift axes; 3.6 -20.9 nm on specimen axes Total_Shift = 10.869242 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 1.63, -0.16 unbinned pixels, unfiltered CCC is 0.0076 The average drift between shots was -0.1, 2.5 unbinned pixels, 0.04 nm/sec Measured defocus = -0.05 microns changed by -1.25 to target 34 frames are being saved to U:\krios\20190806\slot11_1427.tif 34 frames are being saved to U:\krios\20190806\slot11_1428.tif 34 frames are being saved to U:\krios\20190806\slot11_1429.tif Item 546: Stage: 31.98 616.25 83 Label: 544 546 Tick time from program start = 81718.082 Running script 17 at item 545 (33.23, 613.88) Remaining time to fill dewars is 6960 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 120 23 20 170 Trimming A by 20 20 20 20, reference by 20 20 20 90 Trimming A by 20 20 20 20, reference by 20 20 20 110 Disparity in stage position after aligning to center of map frame: -0.22 -0.18 Disparity in stage position after aligning to target position: -0.36 -0.12 (2nd move error -0.15 0.04) Finished aligning to item in 2 rounds Ending Navigator acquisition after current item -0.1 29.1 unbinned pixels; 107.3 43.3 nm along two shift axes; -113.3 -6.7 nm on specimen axes Total_Shift = 58.270584 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 9.15, -4.71 unbinned pixels, unfiltered CCC is 0.0012 The average drift between shots was -6.4, 1.6 unbinned pixels, 0.11 nm/sec Measured defocus = -0.32 microns changed by -1.08 to target 34 frames are being saved to U:\krios\20190806\slot11_1430.tif 34 frames are being saved to U:\krios\20190806\slot11_1431.tif 34 frames are being saved to U:\krios\20190806\slot11_1432.tif Item 547: Stage: 33.23 613.88 83 Label: 545 547 Tick time from program start = 81838.608 Running script 17 at item 546 (44.03, 590.61) Remaining time to fill dewars is 5100 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 150 20 65 Trimming A by 20 20 20 20, reference by 20 158 20 20 Trimming A by 20 20 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.25 -0.09 Disparity in stage position after aligning to target position: -0.18 -0.20 (2nd move error 0.07 -0.13) Finished aligning to item in 2 rounds -17.0 -0.6 unbinned pixels; 20.0 62.1 nm along two shift axes; 5.9 -66.1 nm on specimen axes Total_Shift = 34.050893 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 13.15, -6.94 unbinned pixels, unfiltered CCC is 0.0021 The average drift between shots was -0.8, 5.6 unbinned pixels, 0.10 nm/sec Measured defocus = -0.46 microns changed by -1.04 to target 34 frames are being saved to U:\krios\20190806\slot11_1433.tif 34 frames are being saved to U:\krios\20190806\slot11_1434.tif 34 frames are being saved to U:\krios\20190806\slot11_1435.tif Item 548: Stage: 44.03 590.61 83 Label: 546 Note: s- 548 Tick time from program start = 83648.032 Running script 17 at item 547 (45.11, 588.27) Remaining time to fill dewars is 4860 Dewars are NOT busy filling Trimming A by 20 160 20 20, reference by 20 20 20 20 Trimming A by 20 160 20 20, reference by 20 20 20 20 Trimming A by 20 160 20 20, reference by 20 20 20 20 Trimming A by 20 160 20 20, reference by 20 150 20 65 Trimming A by 20 160 20 20, reference by 20 158 20 20 Trimming A by 20 160 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.10 -0.10 Trimming A by 20 20 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.17 -0.13 (2nd move error -0.06 -0.05) Finished aligning to item in 2 rounds -7.4 14.0 unbinned pixels; 61.0 5.6 nm along two shift axes; -52.8 -31.7 nm on specimen axes Total_Shift = 31.599725 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 14.48, -7.05 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -5.0, 0.2 unbinned pixels, 0.09 nm/sec Measured defocus = -0.50 microns changed by -1.10 to target 34 frames are being saved to U:\krios\20190806\slot11_1436.tif 34 frames are being saved to U:\krios\20190806\slot11_1437.tif 34 frames are being saved to U:\krios\20190806\slot11_1438.tif Item 549: Stage: 45.11 588.27 83 Label: 547 549 Tick time from program start = 83771.055 Running script 17 at item 548 (46.15, 585.91) Remaining time to fill dewars is 4860 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 30 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.14 -0.10 (2nd move error -0.04 -0.02) Finished aligning to item in 1 rounds Trimming A by 20 30 20 20, reference by 20 30 20 20 -4.1 9.3 unbinned pixels; 39.4 0.9 nm along two shift axes; -35.1 -18.0 nm on specimen axes Total_Shift = 20.277078 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 13.91, -7.71 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -4.4, 0.4 unbinned pixels, 0.08 nm/sec Measured defocus = -0.49 microns changed by -1.21 to target 34 frames are being saved to U:\krios\20190806\slot11_1439.tif 34 frames are being saved to U:\krios\20190806\slot11_1440.tif 34 frames are being saved to U:\krios\20190806\slot11_1441.tif Item 550: Stage: 46.15 585.91 83 Label: 548 550 Tick time from program start = 83884.873 Running script 17 at item 549 (43.86, 584.79) Remaining time to fill dewars is 4620 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 60 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.09 -0.12 (2nd move error 0.01 -0.04) Finished aligning to item in 1 rounds Trimming A by 20 60 20 20, reference by 20 60 20 20 -5.9 -1.0 unbinned pixels; 4.0 22.7 nm along two shift axes; 5.1 -22.7 nm on specimen axes Total_Shift = 11.930992 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 14.50, -9.14 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -1.4, 2.7 unbinned pixels, 0.05 nm/sec Measured defocus = -0.53 microns changed by -1.27 to target 34 frames are being saved to U:\krios\20190806\slot11_1442.tif 34 frames are being saved to U:\krios\20190806\slot11_1443.tif 34 frames are being saved to U:\krios\20190806\slot11_1444.tif Item 551: Stage: 43.86 584.79 83 Label: 549 551 Tick time from program start = 83998.598 Running script 17 at item 550 (42.83, 587.19) Remaining time to fill dewars is 4560 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 20 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.16 -0.14 (2nd move error -0.06 -0.06) Finished aligning to item in 1 rounds -9.1 12.9 unbinned pixels; 59.2 13.4 nm along two shift axes; -48.1 -38.1 nm on specimen axes Total_Shift = 31.513113 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 24.61, -14.04 unbinned pixels, unfiltered CCC is 0.0003 The average drift between shots was -6.3, -0.2 unbinned pixels, 0.11 nm/sec Measured defocus = -0.88 microns changed by -1.02 to target 34 frames are being saved to U:\krios\20190806\slot11_1445.tif 34 frames are being saved to U:\krios\20190806\slot11_1446.tif 34 frames are being saved to U:\krios\20190806\slot11_1447.tif Item 552: Stage: 42.83 587.19 83 Label: 550 552 Tick time from program start = 84112.323 Running script 17 at item 551 (30.99, 612.80) Remaining time to fill dewars is 4440 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 120 23 20 170 Trimming A by 20 20 20 20, reference by 20 20 20 90 Trimming A by 20 20 20 20, reference by 20 20 20 110 Disparity in stage position after aligning to center of map frame: -0.31 -0.15 Disparity in stage position after aligning to target position: -0.41 -0.15 (2nd move error -0.10 -0.02) Finished aligning to item in 2 rounds -4.3 21.1 unbinned pixels; 83.3 16.2 nm along two shift axes; -81.2 -21.3 nm on specimen axes Total_Shift = 43.106939 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 27.62, -14.74 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -3.2, 0.1 unbinned pixels, 0.06 nm/sec Measured defocus = -0.97 microns changed by -1.03 to target 34 frames are being saved to U:\krios\20190806\slot11_1448.tif 34 frames are being saved to U:\krios\20190806\slot11_1449.tif 34 frames are being saved to U:\krios\20190806\slot11_1450.tif Item 553: Stage: 30.99 612.80 83 Label: 551 553 Tick time from program start = 84236.078 Running script 17 at item 552 (29.79, 615.24) Remaining time to fill dewars is 4200 Dewars are NOT busy filling Trimming A by 26 20 20 20, reference by 120 23 20 170 Trimming A by 26 20 20 20, reference by 20 20 20 20 Trimming A by 26 20 20 20, reference by 20 20 20 20 Trimming A by 26 20 20 20, reference by 20 20 20 90 Trimming A by 26 20 20 20, reference by 20 20 20 20 Trimming A by 26 20 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.26 -0.15 Disparity in stage position after aligning to target position: -0.23 -0.19 (2nd move error 0.02 -0.06) Finished aligning to item in 2 rounds -8.4 -0.6 unbinned pixels; 8.9 31.1 nm along two shift axes; 4.0 -32.6 nm on specimen axes Total_Shift = 16.846922 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 25.70, -15.51 unbinned pixels, unfiltered CCC is -0.0024 The average drift between shots was 1.2, 6.4 unbinned pixels, 0.11 nm/sec Measured defocus = -0.93 microns changed by -1.17 to target 34 frames are being saved to U:\krios\20190806\slot11_1451.tif 34 frames are being saved to U:\krios\20190806\slot11_1452.tif 34 frames are being saved to U:\krios\20190806\slot11_1453.tif Item 554: Stage: 29.79 615.24 83 Label: 552 554 Tick time from program start = 84356.948 Running script 17 at item 553 (27.61, 619.83) Remaining time to fill dewars is 4140 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.36 -0.19 (2nd move error -0.10 -0.06) Finished aligning to item in 1 rounds -7.3 20.9 unbinned pixels; 86.3 5.1 nm along two shift axes; -79.7 -32.8 nm on specimen axes Total_Shift = 44.228357 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 58.47, -37.96 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -0.9, 2.4 unbinned pixels, 0.05 nm/sec Measured defocus = -2.15 microns changed by -0.05 to target 34 frames are being saved to U:\krios\20190806\slot11_1454.tif 34 frames are being saved to U:\krios\20190806\slot11_1455.tif 34 frames are being saved to U:\krios\20190806\slot11_1456.tif Item 555: Stage: 27.61 619.83 83 Label: 553 555 Tick time from program start = 84470.454 Running script 17 at item 554 (26.53, 622.13) Remaining time to fill dewars is 4020 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 60 20 20 40, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.26 -0.19 (2nd move error -0.00 -0.07) Finished aligning to item in 1 rounds Trimming A by 60 20 20 40, reference by 60 20 20 40 -9.7 1.7 unbinned pixels; 18.9 32.4 nm along two shift axes; -4.6 -38.2 nm on specimen axes Total_Shift = 19.725741 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 23.61, -15.21 unbinned pixels, unfiltered CCC is 0.0017 The average drift between shots was -0.1, 2.8 unbinned pixels, 0.05 nm/sec Measured defocus = -0.87 microns changed by -1.43 to target 34 frames are being saved to U:\krios\20190806\slot11_1457.tif 34 frames are being saved to U:\krios\20190806\slot11_1458.tif 34 frames are being saved to U:\krios\20190806\slot11_1459.tif Item 556: Stage: 26.53 622.13 83 Label: 554 556 Tick time from program start = 84584.070 Running script 17 at item 555 (24.21, 620.83) Remaining time to fill dewars is 4020 Dewars are NOT busy filling Trimming A by 116 21 20 175, reference by 120 23 20 170 Trimming A by 116 21 20 175, reference by 20 20 20 20 Trimming A by 116 21 20 175, reference by 20 20 20 90 Trimming A by 116 21 20 175, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.28 -0.06 Trimming A by 55 21 20 55, reference by 120 23 20 170 Disparity in stage position after aligning to target position: -0.21 0.02 (2nd move error 0.06 0.07) Finished aligning to item in 2 rounds Trimming A by 60 21 35 50, reference by 55 21 20 55 0.4 -0.2 unbinned pixels; 1.1 1.2 nm along two shift axes; 0.6 1.6 nm on specimen axes Total_Shift = 0.865454 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 63.43, -40.50 unbinned pixels, unfiltered CCC is 0.0006 The average drift between shots was -4.3, 8.9 unbinned pixels, 0.17 nm/sec Measured defocus = -2.33 microns changed by -0.07 to target 34 frames are being saved to U:\krios\20190806\slot11_1460.tif 34 frames are being saved to U:\krios\20190806\slot11_1461.tif 34 frames are being saved to U:\krios\20190806\slot11_1462.tif Item 557: Stage: 24.21 620.83 83 Label: 555 557 Tick time from program start = 84706.827 Running script 17 at item 556 (25.32, 618.78) Remaining time to fill dewars is 3780 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.27 -0.22 (2nd move error -0.02 -0.09) Finished aligning to item in 1 rounds -13.8 5.5 unbinned pixels; 38.3 41.2 nm along two shift axes; -18.6 -54.7 nm on specimen axes Total_Shift = 29.664755 Target defocus is: -2.40 um 0.00 degrees Target defocus is: -2.50 um Tilt-induced shift is 35.54, -20.45 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -2.2, 2.9 unbinned pixels, 0.06 nm/sec Measured defocus = -1.27 microns changed by -1.23 to target 34 frames are being saved to U:\krios\20190806\slot11_1463.tif 34 frames are being saved to U:\krios\20190806\slot11_1464.tif 34 frames are being saved to U:\krios\20190806\slot11_1465.tif Item 558: Stage: 25.32 618.78 83 Label: 556 558 Tick time from program start = 84819.896 Running script 17 at item 557 (26.50, 616.52) Remaining time to fill dewars is 3660 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 22 20 20 45, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.13 -0.24 (2nd move error 0.13 -0.11) Finished aligning to item in 1 rounds Trimming A by 35 21 20 40, reference by 22 20 20 45 -13.8 0.8 unbinned pixels; 21.1 48.3 nm along two shift axes; -0.4 -53.8 nm on specimen axes Total_Shift = 27.612526 Target defocus is: -2.50 um 0.00 degrees Target defocus is: -1.30 um Tilt-induced shift is 38.64, -24.09 unbinned pixels, unfiltered CCC is -0.0003 The average drift between shots was -0.0, 8.0 unbinned pixels, 0.14 nm/sec Measured defocus = -1.41 microns changed by 0.11 to target 34 frames are being saved to U:\krios\20190806\slot11_1466.tif 34 frames are being saved to U:\krios\20190806\slot11_1467.tif 34 frames are being saved to U:\krios\20190806\slot11_1468.tif Item 559: Stage: 26.50 616.52 83 Label: 557 559 Tick time from program start = 84933.106 Running script 17 at item 558 (27.69, 614.08) Remaining time to fill dewars is 3600 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.38 -0.21 (2nd move error -0.08 -0.08) Finished aligning to item in 1 rounds -10.3 15.9 unbinned pixels; 71.7 13.1 nm along two shift axes; -59.6 -43.3 nm on specimen axes Total_Shift = 37.797999 Target defocus is: -1.30 um 0.00 degrees Target defocus is: -1.40 um Tilt-induced shift is 7.51, -2.74 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -5.5, -0.1 unbinned pixels, 0.10 nm/sec Measured defocus = -0.24 microns changed by -1.16 to target 34 frames are being saved to U:\krios\20190806\slot11_1469.tif 34 frames are being saved to U:\krios\20190806\slot11_1470.tif 34 frames are being saved to U:\krios\20190806\slot11_1471.tif Item 560: Stage: 27.69 614.08 83 Label: 558 560 Tick time from program start = 85046.535 Running script 17 at item 559 (28.72, 611.75) Remaining time to fill dewars is 3420 Dewars are NOT busy filling Skipping first round alignment to center of map frame Disparity in stage position after aligning to target position: -0.31 -0.20 (2nd move error -0.01 -0.07) Finished aligning to item in 1 rounds -8.7 2.8 unbinned pixels; 21.6 27.2 nm along two shift axes; -9.0 -34.5 nm on specimen axes Total_Shift = 18.310256 Target defocus is: -1.40 um 0.00 degrees Target defocus is: -1.50 um Tilt-induced shift is 13.41, -7.22 unbinned pixels, unfiltered CCC is 0.0018 The average drift between shots was -2.6, 3.1 unbinned pixels, 0.07 nm/sec Measured defocus = -0.47 microns changed by -1.03 to target 34 frames are being saved to U:\krios\20190806\slot11_1472.tif 34 frames are being saved to U:\krios\20190806\slot11_1473.tif 34 frames are being saved to U:\krios\20190806\slot11_1474.tif Item 561: Stage: 28.72 611.75 83 Label: 559 561 Tick time from program start = 85159.308 Running script 17 at item 560 (39.44, 588.35) Remaining time to fill dewars is 3300 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 20 20 20 90 Trimming A by 20 20 20 20, reference by 20 20 20 110 Trimming A by 20 20 20 20, reference by 22 225 20 210 Trimming A by 20 20 20 20, reference by 20 150 20 65 Trimming A by 20 20 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to center of map frame: -0.31 -0.15 Disparity in stage position after aligning to target position: -0.20 0.01 (2nd move error 0.10 0.15) Finished aligning to item in 2 rounds 2.0 -2.0 unbinned pixels; 9.8 4.3 nm along two shift axes; 7.2 8.2 nm on specimen axes Total_Shift = 5.597337 Target defocus is: -1.50 um 0.00 degrees Target defocus is: -1.60 um Tilt-induced shift is 18.86, -9.98 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -7.4, 7.9 unbinned pixels, 0.19 nm/sec Measured defocus = -0.66 microns changed by -0.94 to target 34 frames are being saved to U:\krios\20190806\slot11_1475.tif 34 frames are being saved to U:\krios\20190806\slot11_1476.tif 34 frames are being saved to U:\krios\20190806\slot11_1477.tif Item 562: Stage: 39.44 588.35 83 Label: 560 562 Tick time from program start = 85283.874 Running script 17 at item 561 (40.58, 586.08) Remaining time to fill dewars is 3180 Dewars are NOT busy filling Trimming A by 20 160 20 20, reference by 20 20 20 20 Trimming A by 20 160 20 20, reference by 20 20 20 20 Trimming A by 20 160 20 20, reference by 20 20 20 20 Trimming A by 20 160 20 20, reference by 20 150 20 65 Trimming A by 20 160 20 20, reference by 20 158 20 20 Trimming A by 20 160 20 20, reference by 21 225 115 20 Disparity in stage position after aligning to center of map frame: -0.13 -0.07 Trimming A by 20 20 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.18 -0.17 (2nd move error -0.05 -0.12) Finished aligning to item in 2 rounds -21.6 15.8 unbinned pixels; 86.2 54.0 nm along two shift axes; -56.8 -87.3 nm on specimen axes Total_Shift = 53.482507 Target defocus is: -1.60 um 0.00 degrees Target defocus is: -1.70 um Tilt-induced shift is 19.69, -10.60 unbinned pixels, unfiltered CCC is 0.0005 The average drift between shots was -3.8, 1.4 unbinned pixels, 0.07 nm/sec Measured defocus = -0.69 microns changed by -1.01 to target 34 frames are being saved to U:\krios\20190806\slot11_1478.tif 34 frames are being saved to U:\krios\20190806\slot11_1479.tif 34 frames are being saved to U:\krios\20190806\slot11_1480.tif Item 563: Stage: 40.58 586.08 83 Label: 561 563 Tick time from program start = 85407.661 Running script 17 at item 562 (41.66, 583.70) Remaining time to fill dewars is 3060 Dewars are NOT busy filling Skipping first round alignment to center of map frame Trimming A by 20 89 20 20, reference by 20 158 20 20 Disparity in stage position after aligning to target position: -0.18 -0.18 (2nd move error -0.05 -0.13) Finished aligning to item in 1 rounds Ending Navigator acquisition after current item Trimming A by 20 90 20 20, reference by 20 89 20 20 -16.3 10.6 unbinned pixels; 60.4 42.7 nm along two shift axes; -37.9 -65.6 nm on specimen axes Total_Shift = 38.921828 Target defocus is: -1.70 um 0.00 degrees Target defocus is: -1.80 um Tilt-induced shift is 19.71, -10.59 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -5.1, 0.1 unbinned pixels, 0.09 nm/sec Measured defocus = -0.69 microns changed by -1.11 to target 34 frames are being saved to U:\krios\20190806\slot11_1481.tif 34 frames are being saved to U:\krios\20190806\slot11_1482.tif 34 frames are being saved to U:\krios\20190806\slot11_1483.tif Item 564: Stage: 41.66 583.70 83 Label: 562 564 Tick time from program start = 85524.163 Running script 1 at item 986 (-361.84, 676.68) Remaining time to fill dewars is 2640 Dewars are NOT busy filling Trimming A by 40 21 40 20, reference by 20 20 20 20 Trimming A by 40 21 40 20, reference by 30 20 25 20 Trimming A by 40 21 40 20, reference by 20 20 20 20 Trimming A by 40 21 40 20, reference by 20 20 20 20 Disparity in stage position after aligning to center of map frame: -0.44 -0.19 Trimming A by 20 20 20 20, reference by 30 20 25 20 Disparity in stage position after aligning to target position: -0.46 -0.27 (2nd move error -0.02 -0.10) Finished aligning to item in 2 rounds -14.8 7.1 unbinned pixels; 45.5 42.4 nm along two shift axes; -24.6 -59.0 nm on specimen axes Total_Shift = 32.812292 Target defocus is: -1.80 um 0.00 degrees Target defocus is: -1.90 um Tilt-induced shift is 11.15, -5.68 unbinned pixels, unfiltered CCC is 0.0024 The average drift between shots was -6.8, 11.2 unbinned pixels, 0.23 nm/sec Measured defocus = -0.39 microns changed by -1.51 to target Tilt-induced shift is 50.88, -32.37 unbinned pixels, unfiltered CCC is 0.0001 The average drift between shots was -2.9, 8.9 unbinned pixels, 0.17 nm/sec Measured defocus = -1.86 microns changed by -0.04 to target 34 frames are being saved to U:\krios\20190806\slot11_1484.tif Item 988: Stage: -361.84 676.68 77 Label: 986 988 Tick time from program start = 85900.593 Running script 1 at item 987 (-375.21, 658.55) Remaining time to fill dewars is 2580 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 30 20 25 20 Disparity in stage position after aligning to center of map frame: -0.52 -0.21 Disparity in stage position after aligning to target position: -0.42 -0.24 (2nd move error 0.09 -0.05) Finished aligning to item in 2 rounds -5.7 0.6 unbinned pixels; 9.5 19.5 nm along two shift axes; -1.0 -22.1 nm on specimen axes Total_Shift = 11.368812 Target defocus is: -1.90 um 0.00 degrees Target defocus is: -2.00 um Tilt-induced shift is 68.92, -43.38 unbinned pixels, unfiltered CCC is 0.0020 The average drift between shots was -3.6, 11.0 unbinned pixels, 0.21 nm/sec Measured defocus = -2.52 microns changed by 0.52 to target 34 frames are being saved to U:\krios\20190806\slot11_1485.tif Item 989: Stage: -375.21 658.55 77 Label: 987 989 Tick time from program start = 85978.812 Running script 1 at item 988 (-382.02, 637.70) Remaining time to fill dewars is 2400 Dewars are NOT busy filling Trimming A by 20 60 20 20, reference by 20 20 20 20 Trimming A by 20 60 20 20, reference by 20 20 20 20 Trimming A by 20 60 20 20, reference by 20 20 20 20 Trimming A by 20 60 20 20, reference by 23 110 20 95 Trimming A by 20 60 20 20, reference by 20 60 20 20 Trimming A by 20 60 20 20, reference by 20 70 20 20 Disparity in stage position after aligning to center of map frame: -0.38 -0.17 Trimming A by 20 20 20 20, reference by 20 60 20 20 Disparity in stage position after aligning to target position: -0.36 -0.17 (2nd move error 0.02 -0.03) Finished aligning to item in 2 rounds -6.1 -1.9 unbinned pixels; 1.0 24.7 nm along two shift axes; 8.6 -23.3 nm on specimen axes Total_Shift = 12.726035 Target defocus is: -2.00 um 0.00 degrees Target defocus is: -2.10 um Tilt-induced shift is 19.20, -10.09 unbinned pixels, unfiltered CCC is -0.0005 The average drift between shots was -3.4, 6.0 unbinned pixels, 0.12 nm/sec Measured defocus = -0.67 microns changed by -1.43 to target 34 frames are being saved to U:\krios\20190806\slot11_1486.tif Item 990: Stage: -382.02 637.70 77 Label: 988 990 Tick time from program start = 86058.731 Running script 1 at item 989 (-206.30, 690.60) Remaining time to fill dewars is 2220 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 85 21 100 20 Disparity in stage position after aligning to center of map frame: -0.36 -0.23 Disparity in stage position after aligning to target position: -0.36 -0.26 (2nd move error -0.01 -0.04) Finished aligning to item in 2 rounds -5.8 3.3 unbinned pixels; 19.7 16.1 nm along two shift axes; -11.5 -23.4 nm on specimen axes Total_Shift = 13.394408 Target defocus is: -2.10 um 0.00 degrees Target defocus is: -2.20 um Tilt-induced shift is 61.71, -39.87 unbinned pixels, unfiltered CCC is -0.0000 The average drift between shots was 5.1, 1.5 unbinned pixels, 0.09 nm/sec Measured defocus = -2.27 microns changed by 0.07 to target 34 frames are being saved to U:\krios\20190806\slot11_1487.tif Item 991: Stage: -206.30 690.60 80 Label: 989 991 Tick time from program start = 86291.547 Running script 1 at item 990 (-208.67, 672.23) Remaining time to fill dewars is 2160 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 85 21 100 20 Disparity in stage position after aligning to center of map frame: -0.29 -0.17 Disparity in stage position after aligning to target position: -0.28 -0.27 (2nd move error 0.01 -0.12) Finished aligning to item in 2 rounds -15.9 -0.5 unbinned pixels; 18.9 57.8 nm along two shift axes; 5.2 -61.6 nm on specimen axes Total_Shift = 31.745765 Target defocus is: -2.20 um 0.00 degrees Target defocus is: -2.30 um Tilt-induced shift is 43.55, -24.33 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -0.0, 4.4 unbinned pixels, 0.08 nm/sec Measured defocus = -1.54 microns changed by -0.76 to target 34 frames are being saved to U:\krios\20190806\slot11_1488.tif Item 992: Stage: -208.67 672.23 80 Label: 990 992 Tick time from program start = 86368.893 Running script 1 at item 991 (-203.12, 648.93) Remaining time to fill dewars is 1980 Dewars are NOT busy filling Trimming A by 20 20 20 20, reference by 23 100 95 20 Disparity in stage position after aligning to center of map frame: -0.36 -0.19 Trimming A by 20 70 20 20, reference by 20 20 20 20 Disparity in stage position after aligning to target position: -0.31 -0.18 (2nd move error 0.05 -0.01) Finished aligning to item in 2 rounds Trimming A by 20 70 20 20, reference by 20 70 20 20 -3.0 -0.5 unbinned pixels; 2.0 11.4 nm along two shift axes; 2.6 -11.4 nm on specimen axes Total_Shift = 6.01392 Target defocus is: -2.30 um 0.00 degrees Target defocus is: -2.40 um Tilt-induced shift is 34.22, -21.25 unbinned pixels, unfiltered CCC is 0.0007 The average drift between shots was -1.4, 7.1 unbinned pixels, 0.13 nm/sec Measured defocus = -1.25 microns changed by -1.15 to target 34 frames are being saved to U:\krios\20190806\slot11_1489.tif Item 993: Stage: -203.12 648.93 80 Label: 991 993 Tick time from program start = 86448.828