Krios1 Quantum-K2 Cs 2.7 300kV EFTEM nanpprobe x130k spot 8 beam size 620nm c2=50 objective_aperture=100 GIF-K2 counting 12s exposure 40 frames total dose at the sample 42.70e/A2 1 pixel = 1.04A dose rate ~3 e/pix/s defocus range -1.2 to -2.5