Movies (FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth. The compression is lossless in that all pixel intensities remain exactly the same after decompression. However, some metadata in the MRC header were lost. The original files before compression are available from EMPIAR EBI. Compression was carried out by separating (“un-normalizing”) the gain. The details of the method are described in https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies. Please apply the gain reference (gain-reference.mrc) during processing. The original deposition contained a file ".FoilHole_2991125_Data_2991864_2991865_20181103_1811-111065.mrc.o7i2AF", which is a temporary file generated by rsync. This file was removed. Original size: 2,603,065,148 kilobytes Compressed size: 291,978,494 kilobytes