index	label	resource	url	accession	description
1	cryo-electron microscopy	ero	http://purl.obolibrary.org/obo/ERO_0001773	ERO_0001773	A form of transmission electron microscopy where the sample is studied at cryogenic temperatures (generally liquid nitrogen temperatures).
2	cryo-electron microscopy	fix	http://purl.obolibrary.org/obo/FIX_0000052	FIX_0000052	-
3	Cryo-Electron Microscopy	ncit	http://purl.obolibrary.org/obo/NCIT_C18113	NCIT_C18113	Electron microscopy involving an aqueous suspension of a sample which is applied in an extremely thin film to a grid, frozen in liquid nitrogen and maintained in this state by means of a special mount. The imaging of frozen- hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.
4	electron tomography	ero	http://purl.obolibrary.org/obo/ERO_0001786	ERO_0001786	An electron microscopy method for three- dimensional (3D) reconstruction of single, transparent objects from a series of projection images (i.e. from a tilt series) recorded with a transmission electron microscope. It offers the opportunity to obtain 3D information on structural cellular arrangements with a high resolution.
5	electron tomography	mi	http://purl.obolibrary.org/obo/MI_2338	MI_2338	Three-dimensional (3D) reconstruction of single, transparent objects from a series of projection images from a tilt series recorded with a transmission electron microscope.
6	Tomography	edam	http://edamontology.org/topic_3452	topic_3452	Imaging in sections (sectioning), through the use of a wave-generating device (tomograph) that generates an image (a tomogram).
7	cryogenic electron microscopy	chmo	http://purl.obolibrary.org/obo/CHMO_0002413	CHMO_0002413	Microscopy where the specimen, which is cooled in liquid ethane to 180 °C, is bombarded with a finely focused (<10 nm diameter) electron beam with an acceleration voltage 50–150 kV under vacuum and the interaction of the electrons with the specimen produces transmitted, secondary, backscattered and diffracted electrons and characteristic X-rays which are detected.
8	cryogenic scanning electron microscopy	chmo	http://purl.obolibrary.org/obo/CHMO_0002589	CHMO_0002589	Microscopy where a finely focused (<10 nm diameter) electron beam with an acceleration voltage 50–150 kV is scanned across an electron transparent specimen under vacuum and the intensities of the transmitted electrons are measured. Microscopy where a finely focused (<10 nm diameter) electron beam with an acceleration voltage 50–150 kV is scanned across the specimen, which is cooled in liquid ethane to 180 °C, under vacuum and the interaction of the electrons with the specimen is determined.
9	Electron Microscope Tomography	omit	http://purl.obolibrary.org/obo/OMIT_0026117	OMIT_0026117	-
10	cryogenic transmission electron microscopy	chmo	http://purl.obolibrary.org/obo/CHMO_0000081	CHMO_0000081	Transmission electron microscopy where the specimen is cooled in liquid ethane to 180 °C.