EMBL Heidelberg Krios1 Quantum-K2 Cs 2.7 300kV EFTEM nanpprobe x130k spot 7 beam size 650nm c2=50 objective_aperture=100 GIF-K2 counting 8s exposure 40 frames total dose at the sample 49.36e/A2 1 pixel = 1.04A dose rate ~5 e/pix/s defocus range -1.0 to -2.0