RAW MICROGRAPHS AND REFINEMENT STAR FILE FOR ACA2 BOUND TO RNA (EMD-43762; PDB 8W35)
Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM

Voltage		300 keV
Camera		Gatan K3, super-resolution mode with 2-fold binning by EPU
Energy filter	GIF, 20eV slit width
Magnification	165000
Pixel size	0.531 A/pix
Dose		72.2 e/A2
Exposure rate	15.3 e/pix/s
Exposure time	1.33 s
Fractions	40

Total mics	11232


GainReference.mrc was binned using 'rebin by two' in Digital Micrograph. It needs to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can probably be used as is for cryoSPARC.

particles.star contains metadata for the particle selected for the final reconstruction.