Movies (Raw_movies/*/*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) in each folder during processing.
Note that movies in the Movie2 folder of the original deposition had been corrected by three gain references,
so we divided them into three folders.

Original size:   5,077,103,148 kilobytes
Compressed size:   758,400,164 kilobytes