#Cryo-electron tomography data acquired on S. Cerevisiae (BY4741) cryo-FIB lamellae.

Deposition of 14 tomograms (4 for control cells in glucose rich media, and 10 for the acute glucose starvation condition) used to determine polysome assembly states.

Naming for tomograms of control cells: TS_006, TS_007, TS_008, TS_017 
Naming for tomograms of cells under glucose starvation: TS_001-4, TS_007, TS_008, TS_010, TS_014, TS_017, TS_018

Raw, unaligned multi-frame micrographs for each tilt of the acquired tilt-series are located in frames folders (.tif file format). Corresponding count reference file (.dm4) is provided in the frames folder of the control. Frames were combined into tilt-series stacks in Warp (.st files in metadata folders). 
Tomograms (4x binned, 13.7 A voxel size) were reconstructed using AreTomo (with metadata files mdocs_ori in frames folders, .tif files in frames folder). Subomogram reconstruction was performed in Warp utilizing modified acquisition metadata files (.mdocs modified to exclude bad tilts in metadata/mdocs_modified folders) and alignment files (.mdoc and .xf files in metadata folders).

Cleaned particle lists and 3D annotation volumes for ribosomes localised in 4x binned reconstructed tomograms (13.7 A voxel size) are provided in the labels folders.