<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13728" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-02-19</depositionDate>
            <releaseDate>2026-10-02</releaseDate>
            <updateDate>2026-10-02</updateDate>
        </keyDates>
        <title>JMV449-NTSR1-Gi in 8 seconds following the GTP addition</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-3366-3586</authorORCID>
            <firstName>Kazuhiro</firstName>
            <lastName>Kobayashi</lastName>
            <organization type="academic">Research Center for Advanced Science and Technology, The University of Tokyo</organization>
            <street>4-6-1 Komaba</street>
            <townOrCity>Meguro</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>153-8904</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-3366-3586</authorORCID>
            <firstName>Kazuhiro</firstName>
            <lastName>Kobayashi</lastName>
            <organization type="academic">Research Center for Advanced Science and Technology, The University of Tokyo</organization>
            <street>4-6-1 Komaba</street>
            <townOrCity>Meguro</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>153-8904</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <firstName>Kouki</firstName>
            <lastName>Kawakami</lastName>
            <organization type="academic">Research Center for Advanced Science and Technology, The University of Tokyo</organization>
            <street>4-6-1 Komaba</street>
            <townOrCity>Meguro</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>153-8904</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <firstName>Masahiro</firstName>
            <lastName>Fukuda</lastName>
            <organization type="academic">Research Center for Advanced Science and Technology, The University of Tokyo</organization>
            <street>4-6-1 Komaba</street>
            <townOrCity>Meguro</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>153-8904</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <firstName>Keitaro</firstName>
            <lastName>Yamashita</lastName>
            <organization type="academic">Research Center for Advanced Science and Technology, The University of Tokyo</organization>
            <street>4-6-1 Komaba</street>
            <townOrCity>Meguro</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>153-8904</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <firstName>Hideaki</firstName>
            <lastName>Kato</lastName>
            <organization type="academic">Research Center for Advanced Science and Technology, The University of Tokyo</organization>
            <street>4-6-1 Komaba</street>
            <townOrCity>Meguro</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>153-8904</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-3366-3586">Kobayashi K</author>
            <author authorORCID="0000-0002-2507-8619">Kawakami K</author>
            <author>Matsui TE</author>
            <author authorORCID="0000-0002-8824-846X">Fukuda M</author>
            <author authorORCID="0000-0002-5442-7582">Yamashita K</author>
            <author authorORCID="0000-0001-5958-3990">Kobilka BK</author>
            <author authorORCID="0000-0002-1941-5535">Kato HE</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">2.7</datasetSize>
        <entryDOI>10.6019/EMPIAR-13728</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-64917</emdbEntry>
            <emdbEntry>EMD-64918</emdbEntry>
            <emdbEntry>EMD-64919</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-3366-3586" order="1">Kobayashi K</author>
                    <author authorORCID="0000-0002-2507-8619" order="2">Kawakami K</author>
                    <author order="3">Matsui TE</author>
                    <author order="4">Yokoi S</author>
                    <author authorORCID="0000-0002-8824-846X" order="5">Fukuda M</author>
                    <author order="6">Narita TJ</author>
                    <author order="7">Arai H</author>
                    <author order="8">Tambo M</author>
                    <author authorORCID="0000-0003-3696-5720" order="9">Sumikama T</author>
                    <author order="10">Tatsumi M</author>
                    <author authorORCID="0000-0002-5442-7582" order="11">Yamashita K</author>
                    <author order="12">Koyanagi J</author>
                    <author order="13">Kugawa M</author>
                    <author order="14">Ikeda H</author>
                    <author order="15">Sumino A</author>
                    <author authorORCID="0000-0002-4194-7255" order="16">Mitsutake A</author>
                    <author authorORCID="0000-0001-5958-3990" order="17">Kobilka BK</author>
                    <author authorORCID="0000-0003-0805-4049" order="18">Inoue A</author>
                    <author authorORCID="0000-0002-1941-5535" order="19">Kato HE</author>
                    <title>The dynamic basis of G-protein recognition and activation by a GPCR</title>
                    <journal>Nature</journal>
                    <journalAbbreviation>Nature</journalAbbreviation>
                    <country></country>
                    <issue>8110</issue>
                    <volume>652</volume>
                    <firstPage>812</firstPage>
                    <lastPage>821</lastPage>
                    <year>2026</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41586-026-10228-w</externalReferences>
                    <externalReferences type="pubmed">41813902</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>imageset_1</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>12138</numImagesOrTiltSeries>
        <framesPerImage>48</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>48</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>- Please ignore the pixel size in the header (1.00 Å/px); use 0.83 Å/px.
- Cs: 2.7mm
- HT: 300 kV
- Dose per frame: 1 electron/A^2/frame
- Please use "movie_gain.mrc" as a gain reference with flipping along Y for CryoSPARC</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
