<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10213" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2018-04-05</depositionDate>
            <releaseDate>2019-04-05</releaseDate>
            <updateDate>2022-04-21</updateDate>
        </keyDates>
        <title>mouse MDA5-dsRNA Filaments in presence of 1mM AMPPNP</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-6084-0429</authorORCID>
            <firstName>Yorgo</firstName>
            <lastName>Modis</lastName>
            <organization type="academic">Department of Medicine, MRC-LMB, University of Cambridge</organization>
            <street>Francis Crick Avenue, Cambridge Biomedical Campus</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 0QH</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-6084-0429</authorORCID>
            <firstName>Yorgo</firstName>
            <lastName>Modis</lastName>
            <organization type="academic">Department of Medicine, MRC-LMB, University of Cambridge</organization>
            <street>Francis Crick Avenue, Cambridge Biomedical Campus</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB20QH</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-9265-632X">Yu Q</author>
            <author authorORCID="0000-0002-3152-2237">Qu K</author>
            <author authorORCID="0000-0002-6084-0429">Modis YE</author>
        </authorsList>
        <datasetSize units="TB">3.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-10213</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-4338</emdbEntry>
            <emdbEntry>EMD-4340</emdbEntry>
            <emdbEntry>EMD-4341</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-9265-632X" order="1">Yu Q</author>
                    <author authorORCID="0000-0002-3152-2237" order="2">Qu K</author>
                    <author authorORCID="0000-0002-6084-0429" order="3">Modis Y.E</author>
                    <title>Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis</title>
                    <journal>Molecular cell</journal>
                    <journalAbbreviation>Mol. Cell</journalAbbreviation>
                    <country>United States</country>
                    <issue>6</issue>
                    <volume>72</volume>
                    <firstPage>999</firstPage>
                    <lastPage>1012.e6</lastPage>
                    <year>2018</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1016/j.molcel.2018.10.012</externalReferences>
                    <externalReferences type="pubmed">30449722</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>mouse MDA5 and bacteriophage dsRNA filaments in complex with 1mM AMPPNP_data1</name>
        <directory>/data/micrographs</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>891</numImagesOrTiltSeries>
        <framesPerImage>75</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>75</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.07</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.07</pixelHeight>
        </dimensions>
        <details>Unaligned raw movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP
Total dose was 29.85 electrons Å -2 over 60 seconds. Calibrated pixel size is 1.07 Å/pixel.
Picked particles file are raw particles from RELION Autopick. Not final particles for reconstruction.</details>
        <segmentationList/>
        <micrographsFilePattern>data/micrographs/*_Fractions.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/micrographs_autopick/*autopick.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/micrographs_autopick</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP_data2</name>
        <directory>/data/micrographs2</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>421</numImagesOrTiltSeries>
        <framesPerImage>75</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>75</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.07</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.07</pixelHeight>
        </dimensions>
        <details>Unaligned raw movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP.
Total dose was 29.85 electrons Å -2 over 60 seconds. Calibrated pixel size is 1.07 Å/pixel.
Picked particles file are raw particles from RELION Autopick. Not final particles for reconstruction.</details>
        <segmentationList/>
        <micrographsFilePattern>data/micrographs2/*_Fractions.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/micrographs2_Autopick/*auto*.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/micrographs2_Autopick</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP_data3</name>
        <directory>/data/micrographs3_1mMAMPPNP</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>210</numImagesOrTiltSeries>
        <framesPerImage>75</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>75</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.07</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.07</pixelHeight>
        </dimensions>
        <details>Unaligned raw movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP
Total dose was 29.85 electrons Å -2 over 60 seconds. Calibrated pixel size is 1.07 Å/pixel.
Picked particles file are raw particles from RELION Autopick. Not final particles for reconstruction.</details>
        <segmentationList/>
        <micrographsFilePattern>data/micrographs3_1mMAMPPNP/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>*/micrographs3_Autopick/*autopick.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/micrographs3_Autopick</pickedParticlesDirectory>
    </imageSet>
</entry>
