REL 2019-02-11 2019-03-25 2019-04-12 0000-0001-6653-6682 Mark Anthony Herzik Jr. The Scripps Research Institute 10550 N. Torrey Pines Rd La Jolla CA United States 92037 0000-0003-4921-1135 Gabriel C Lander The Scripps Research Institute 10550 N. Torrey Pines Rd La Jolla CA United States 92037 Herzik Jr MA Wu M Lander GC 5.5 10.6019/EMPIAR-10252 EMDB EMD-0409 Herzik Jr MA Wu M Lander GC Lander GC Wu M Herzik Jr MA bioRxiv 2018 English 10.1101/489898

Raw, unaligned frame stacks (44 frames) of the catalytic subunit of protein kinase A bound to ATP, manganese, and protein kinase inhibitor (IP20) in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector operating in counting mode - stage position used for exposure navigation. Movies were acquired in counting mode over 11 s with an exposure rate of 1.95 electrons per pixel per second, yielding a total exposure of ~69 electrons per squared Å (1.57 electrons per squared Å per frame), and a nominal defocus range of -0.6 μm to -1.8 μm. Data were collected over three separate sessions – two sessions without tilting the specimen (18feb27c and 18mar03b) and one session while tilting the specimen 30˚ (18aug21a). Images used for normalizations are also included (require flip in Y axis).

Raw, unaligned movie stacks of the catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 acquired on a Talos Arctica using a K2 direct electron detector /data micrographs - multiframe MRC MRC 4812 44 0 43 UNSIGNED 16 BIT INTEGER 3710 0.5585 3838 0.5585

Raw, unaligned counting mode frame stacks (44 frames) of the catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector. Three separate data collections – 18feb27c (collected without tilt), 18mar03b (collected without tilt), and 18aug21a (collected at a 30˚ tilt) – that were combined are provided. Images used for corrections are also provided (require flip in Y).