REL 2020-02-25 2021-01-22 2022-02-08 0000-0003-3550-6274 Mikhail Kudryashev Max-Planck-Institut für Biophysik Max von Laue Straße 3 Frankfurt am Main Germany 60438 0000-0003-3550-6274 Mikhail Kudryashev Max-Planck-Institut für Biophysik Max von Laue Straße 3 Frankfurt am Main Germany 60438 Zhang Y Dijkman PM Zou R Zandl-Lang M Sanchez RM Eckhardt-Strelau L Köfeler H Vogel H Yuan S Kudryashev M 15.6 10.6019/EMPIAR-10381 EMDB EMD-10692 EMD-10691 EMD-10693 Zhang Y Dijkman PM Zou R Zandl-Lang M Sanchez RM Eckhardt-Strelau L Köfeler H Vogel H Yuan S Kudryashev M Nature communications Nat Commun 1 12 2021 English 10.1038/s41467-021-21016-7 33594077

Two raw datasets in the presence and absence of serotonin for the serotonin-bound open-state form and two apo-state forms. The micrographs are original raw data without any alignment applied, 30 electron total exposure over 69 raw movie frames, at pixel size 0.832 Å. Two datasets- Dataset1 for serotonin-bound open state form; Dataset2 for apo-state forms.

Lipid-stabilised 5-HT3A serotonin receptor in asymmetric open state /data/micrographs-Dataset1-serotonin-bound micrographs - multiframe MRC MRC 2261 69 1 69 SIGNED 16 BIT INTEGER 4096 0.832 4096 0.832

The particles are the refined particles in the final asymmetric open map. Name called - 251k_particles.mrcs 251k_particles.star They are generated using relion_stack_create, taking the run_data.star as input. The particle stack mrcs file is in a single stack containing all refined 251k particles for the asymmetric open state map. The micrographs are raw data, without any realignment, of 69 raw movie frame (accumulating 30 electron per squared Angstrom using Falcon III camera in a ~32 second exposure.)

data/micrographs-Dataset1-serotonin-bound/FoilHole_19230112_Data_19227416_19227418_20190719_135918_Fractions.mrc data/particle_datasets/251k_particles.star data/particle_datasets Lipid-stabilised 5-HT3A serotonin receptor in apo state forms (symmetric C5 apo and asymmetric C1 apo) /data/micrographs-Dataset2-apo micrographs - multiframe MRC MRC 5053 69 1 69 SIGNED 16 BIT INTEGER 4096 0.832 4096 0.832

The particles are the refined particles in the final asymmetric apo state map and symmetric apo state map. Names called - (1) 152k_particles.mrcs and 152_particles.star for asymmetric apo (C1) state map; (2) 238k_particles.mrcs and 238_particles.star for symmetric apo (C5) state map. They are generated using relion_stack_create, taking the run_data.star for each job respectively as input. The two particle stack mrcs files are in a single stack containing all refined particles for the asymmetric apo state map and symmetric apo state map, accordingly. The micrographs are raw data, without any realignment, of 69 raw movie frame (accumulating 30 electron per squared Angstrom using Falcon III camera in a ~32 second exposure.)

data/micrographs-Dataset2-apo/FoilHole_14205953_Data_14206374_14206376_20190914_111708_Fractions.mrc data/particle_datasets/238k_particles.star data/particle_datasets