REL
2020-03-26
2020-06-30
2020-06-30
Structural basis for strand-transfer inhibitor binding to HIV intasomes
0000-0003-1333-9102
Dario
Oliveira Passos
Salk Institute
10010 N Torrey Pines
San Diego
California
United States
92037
0000-0002-8124-7472
Dmitry
Lyumkis
Salk Institute
10010 N Torrey Pines
San Diego
California
United States
92037
Passos DO
Li M
Jóźwik IK
Zhao XZ
Santos-Martins D
Yang R
Smith SJ
Jeon Y
Forli S
Hughes SH
Burke TR
Craigie R
Lyumkis D
4.4
10.6019/EMPIAR-10387
EMDB
EMD-20484
Passos DO
Li M
Jóźwik IK
Zhao XZ
Santos-Martins D
Yang R
Smith SJ
Jeon Y
Forli S
Hughes SH
Burke TR
Craigie R
Lyumkis D
Structural basis for strand-transfer inhibitor binding to HIV intasomes
Science (New York, N.Y.)
Science
United States
6479
367
810
814
2020
English
10.1126/science.aay8015
32001521
Compressed movies of HIV intasomes bound to the compound 4d. Movies contain 80 raw frames each, accumulating 45 electron per square Angstrom in an 8 second exposure. Pixel size is 0.79.
Aligned, summed and exposure weighted micrographs of HIV-1 intasome bound to compound 4d.
/data/Sums
micrographs - single frame
MRC
MRC
2022
77
32 BIT FLOAT
3710
0.79
3838
0.79
Aligned, summed and exposure weighted micrographs of HIV-1 intasome bound to compound 4d. Micrographs were aligned and summed using MotionCor2, with 5 by 5 patch and exposure weighting. The first 3 frames, which had the most beam-induced movement, were discarded.
Regarding particle stack of HIV-1 intasome bound to compound 4d. Particles were extracted from aligned, exposured weighted and summed micrographs. This stack contains 138,770 particles (384 x 384 pixels) and was processed in cisTEM. Both *mrc and *par files are included.
data/Sums/*DW.mrc
data/Particle-Stack/18jul30a_4d_F3-77_vol180-1_2-88A.mrc
data/Particle-Stack
Unaligned raw movies of HIV-1 intasome bound to compound 4d
/data/Rawdata
micrographs - multiframe
MRC
MRC
2024
80
UNSIGNED 16 BIT INTEGER
3710
0.79
3838
0.79
Unaligned raw multi-frame movies of HIV-1 intasome bound to compound 4d. Movies were collected with a stage tilt angle set to 20, 30, or 40 degrees. The gain normalization reference image (norm) is included for each of the 3 sessions.