REL 2020-04-03 2020-06-08 2020-10-05 0000-0003-3834-5808 Daniel L. Hurdiss Cryo-Electron Microscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University Padualaan 8 Utrecht Utrecht Netherlands 3584 CH 0000-0002-2207-9472 Raoul J. de Groot Department of Infectious Diseases and Immunology, Virology Division Yalelaan 1 Utrecht Utrecht Netherlands 3584 CL Hurdiss DL Drulyte I 5.0 10.6019/EMPIAR-10390 EMDB EMD-10676 Hurdiss DL Drulyte I Lang Y Shamorkina TM Pronker MF van Kuppeveld FJM Snijder J de Groot RJ Nature communications Nat Commun United States 1 11 2020 English 10.1038/s41467-020-18440-6 32938911

Unaligned gain-normalised movies in standard mrc format. Movies comprise 27 fractions collected over 5.6 seconds with a cumulative exposure of 40 electrons per square angstrom. Data was collected on Krios G4 TEM fitted with a Falcon 4 camera using fringe-free illumination and aberration-free image shifts. The HKU1 HE ectodomain was expressed as an Fc fusion protein in HEK293T cells and purified by protein A affinity chromatography, followed by on-the-bead thrombin cleavage to remove the Fc domain, and size exclusion chromatography

Hurdiss DL Drulyte I Lang Y Shamorkina TM Pronker MF van Kuppeveld FJ Snijder J de Groot RJ bioRxiv 2020 10.1101/2020.03.25.998963 Unaligned multi-frame gain-normalised movies of HCoV-HKU1 HE /data micrographs - multiframe MRC MRC 6029 27 SIGNED 16 BIT INTEGER 4096 0.805 4096 0.805